| Entries |
| Document | Title | Date |
|---|
| 20080199854 | Methods and compositions for identifying RNA-binding proteins - The present invention includes compositions, methods and kits for the identification of a polypeptide that binds to a predetermined RNA sequence. The invention comprises, in part, a photoreactive moiety to aid in identification of such a polypeptide. | 08-21-2008 |
| 20080199855 | Identification And Characterization Of A Subset of Glioblastomas Sensitive To Treatment With Imatinib - The present invention relates to methods for in vitro diagnosing a cell proliferative disease in a mammal, for predicting the behaviour of a mammal having a cell proliferative disease in response to a medical treatment using at least one PDGF receptor antagonist, and for selecting a mammal having a cell proliferative disease and predicted to be responsive to a medical treatment using at least one PDGF receptor antagonist, by using given genetic markers. | 08-21-2008 |
| 20080199856 | Probe for Diagnosis of Marfan Syndrome and a Method for Screening Using the Probe - The purpose of this invention is to provide a probe for diagnosis of Marfan syndrome, which enables early diagnosis of Marfan syndrome, and to provide a method for screening using said probe. The invention is a probe for a Marfan Syndrome characterized by using a nucleic acid comprising following (a) or (b);
| 08-21-2008 |
| 20080199857 | METHOD OF INCREASING SPECIFICITY OF NUCLEIC ACID HYBRIDIZATION USING ZWITTERIONIC COMPOUND - A method of increasing the specificity of nucleic acid hybridization, comprising hybridizing nucleic acid in a solution containing a zwitterionic compound selected from the group consisting of 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS), 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (CHAPS), 3-(cyclohexylamino)-2-hydroxy-1-propane sulfonate (CAPSO), and 2-(cyclohexylamino)ethane sulfonate (CHES) is provided. The method allows a reduction in the yield of non-specific amplification products in multiplex PCRs while maintaining the yield of target amplification products. | 08-21-2008 |
| 20080199858 | Method for Detecting Estrogen-Like Chemicals by Plant | 08-21-2008 |
| 20080199859 | Identification Marker Responsive to Interferon Therapy for Renal Cell Cancer - The present invention provides an identification marker responsive to IFN therapy for renal cell cancer and a means of detecting the same. Namely, a method which comprises preparing a genomic DNA of a human gene or a complementary strand thereof from a specimen of a patient with renal cell cancer, analyzing the DNA sequence of the genomic DNA or the complementary strand thereof to determine the gene polymorphism of the human gene, and evaluating the tumor-suppression effect of IFN therapy on renal cell cancer by using the polymorphism as an indicator. | 08-21-2008 |
| 20080199860 | VARIANTS OF THE ALPHA 1 SUBUNIT OF HUMAN AMPK - Variants of the α subunit of AMPK, nucleic acids encoding such variants, and methods for their use are provided. | 08-21-2008 |
| 20080199861 | Real-time microarray apparatus and methods related thereto - Embodiments of the invention relate to a real-time microarray apparatus comprising an upper substrate, a lower substrate, a buffer positioned between the upper and lower substrate, a microarray positioned on either the upper substrate or the lower substrate, a heater positioned near the microarray, a pump positioned near the buffer and microarray and an imaging sensor positioned near the microarray. | 08-21-2008 |
| 20080199862 | Active biochip for nucleic acid analysis - Embodiments of the invention relate to an active biochip for nucleic acid analysis. The biochip comprises an inlet for introducing a nucleic acid sample, fluid channels, valves in contact with the fluid channels and pumps in contact with the fluid channels and adapted to generate a carrier gas or move a buffer through a portion of the fluid channels. The biochip also includes one or more hydroxyapatite columns for separating a portion of the nucleic acid sample, buffer reservoirs in contact with the fluid channels and positioned near the pumps, air exits, a waste reservoir and a nucleic acid analysis region. | 08-21-2008 |
| 20080199863 | PROBES AND METHODS FOR DETECTION OF PATHOGENS AND ANTIBIOTIC RESISTANCE - Described are probes and methods for detecting pathogens and antibiotic resistance of a specimen. The method comprises contacting the specimen with a growth medium; and lysing the specimen to release nucleic acid molecules from the specimen. The lysate of the specimen is contacted with a capture probe immobilized on a substrate, wherein the capture probe comprises an oligonucleotide that specifically hybridizes with a first target nucleic acid sequence region of ribosomal RNA. The lysate is in contact with a detector probe that comprises a detectably labeled oligonucleotide that specifically hybridizes with a second target nucleic acid sequence region of ribosomal RNA. The presence or absence of labeled oligonucleotide complexed with the substrate is determined. Detection of labeled oligonucleotide complexed with the substrate is indicative of the presence of pathogen. Performing the method in the presence and absence of an antibiotic permits detection of antibiotic resistance. | 08-21-2008 |
| 20080199864 | METHODS FOR CELL SCREENING OF COMPOUNDS CAPABLE OF MODULATING THE ACTIVITY OF UBIQUITIN-LIGASE SCF COMPLEXES AND THEIR USES - The invention concerns methods for cell screening of agents capable of modulating the activity of SCF.sup.Met30 complexes comprising the following steps: (i) contacting the product to be tested with a modified yeast strain, including (a) a hybrid sequence comprising a sequence coding for a Met4 protein, in its wild or mutated form, fused in phase with at least a sequence coding for an appropriate marker, said hybrid sequence being expressed under the control of a promoter, active in the yeast and optionally (b) a reporter transcriptional system, consisting of a reporter gene placed under the control of an appropriate operating sequence or an appropriate yeast promoter, (ii) adding methionine and (iii) determining the level of expression and stability of the expressed protein from the hybrid sequence, and their uses. The invention also concerns plasmids and yeast strains capable of being used in said methods. | 08-21-2008 |
| 20080199865 | Methods for Detecting and Treating the Early Onset of Aging-Related Conditions - Certain aspects of the invention relate to methods for determining a subject's susceptibility to the early onset or progression of aging-related conditions. In certain aspects the invention relates to accessing the genotype of a subject with respect to an allele of IL-1 pattern 1, pattern 2 and/or pattern 3. In other aspects, the invention relates to methods for selecting a therapeutic regimen, identifying age-related biomarkers, monitoring the progress of age-related conditions and identifying therapeutics for delaying or diminishing the onset of aging-related conditions. | 08-21-2008 |
| 20080199866 | SNP DETECTION AND OTHER METHODS FOR CHARACTERIZING AND TREATING BIPOLAR DISORDER AND OTHER AILMENTS - The present application relates to the use of SNPs and differential exon expression to characterize, diagnose or treat bipolar disorder and other mental illnesses, such as major depressive disorder and schizophrenia. | 08-21-2008 |
| 20080199867 | MULTIPLE MODE MULTIPLEX REACTION QUENCHING METHOD - Methods for balancing multiplexed PCR reactions are provided which exploit differences in primer and amplicon Tms. The methods may be controlled by a computer process. Also provided are articles of manufacture useful in such methods and compositions containing primers and probes useful in such methods. | 08-21-2008 |
| 20080199868 | Massive parallel method for decoding DNA and RNA - This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose. | 08-21-2008 |
| 20080199869 | Novel mycobacterium tuberculosis protein composition - proteins and protein compositions that are components of a desaturase complex are provided. The | 08-21-2008 |
| 20080199870 | COMPOSITIONS AND METHODS FOR THE IDENTIFICATION OF INHIBITORS OF PROTEIN SYNTHESIS - Compositions and methods for identifying inhibitors of RNA-target molecule interactions are provided as well as identifying inhibitors that block the role of tRNA in protein synthesis. The methods involve forming a mixture comprising a tRNA fragment molecule containing a modified nucleotide, a target molecule capable of binding to the tRNA fragment, and a test compound. The mixture is incubated under conditions that allow binding of the tRNA and the target molecule in the absence of the test compound. Assays can then be performed that detect whether or not the test compound inhibits the binding of the tRNA molecule and the target molecule. High throughput assays are also provided. | 08-21-2008 |
| 20080199871 | Systems and methods of analyzing nucleic acid polymers and related components - Systems and methods of identifying, sequencing and/or detecting nucleic acid polymers, as well as related components (e.g., substrates, software and the like) are disclosed. | 08-21-2008 |
| 20080199872 | Method and Kit for Analyzing a Target Nucleic Acid Sequence - This invention discloses methods for determining the presence of a target nucleic acid sequence using oligonucleotides that cooperate in a nucleic acid processing reaction to produce a detectable signal. In some embodiments, the methods also facilitate quantification of a target nucleic acid sequence. The present invention further discloses kits that can be used to conduct the methods of the present invention. | 08-21-2008 |
| 20080199873 | Companion diagnostic assays for cancer therapy - Methods for identifying cancer patients eligible to receive Bcl-2 family inhibitor therapy and for monitoring patient response to Bcl-2 family inhibitor therapy comprise assessment of the expression levels of the biomarker combinations set out in TABLES 1, 2, 3, 4, 5 or 6 in a patient tissue sample. The methods of the invention allow more effective identification of patients to receive Bcl-2 family inhibitor therapy and of determination of patient response to the therapy. | 08-21-2008 |
| 20080199874 | CONTROLLABLE STRAND SCISSION OF MINI CIRCLE DNA - The invention provides methods and compositions for the controlled termination of polymerase mediated primer extension reactions. The methods and compositions of the invention are broadly useful, and in a preferred aspect can be used in identifying sequence elements of template nucleic acids. Control of termination not only provides temporal control over termination, but, when used in conjunction with optically confined reaction regions, also spatially controls such termination. | 08-21-2008 |
| 20080199875 | UNSYMMETRICAL CYANINE DIMER COMPOUNDS AND THEIR APPLICATION - Embodiments of the present invention provide methods and nucleic acid reporter molecules for the detection of nucleic acid in a sample. The nucleic acid reporter molecule comprises two unsymmetrical cyanine monomer moieties, which may be the same or different, that are covalently attached by a linker comprising at least one aromatic, heteroaromatic, cyclic or heterocyclic moiety comprising 3-20 non-hydrogen atoms selected from the group consisting of O, N, S, P and C. The linker may be rigid, relatively flexible or some degree thereof. The unsymmetrical cyanine monomer moieties comprise a substituted or unsubstituted benzazolium moiety and a substituted or unsubstituted pyridinium or quinolinium moiety that is connected by a methine bridge that is monomethine, trimethine or pentamethine. The linkers form the cyanine dimer compounds by attaching to the pyridinium or quinolinium moiety of the monomer moieties. The present nucleic acid reporter molecules find utility in forming a nucleic acid-reporter molecule complex and detecting the nucleic acid. In particular, present nucleic acid reporter molecules with a rigid linker and monomer moieties with a monomethine bridge find utility in detecting RNA in the presence of DNA. | 08-21-2008 |
| 20080199876 | Method of Detection of Alterations in MSH5 - We have now discovered that mammals have a DNA gene analogous to that existing in bacteria. MSH5 defects or alterations in this mismatch repair pathway in a mammal, such as a human can be diagnostic of a predisposition to cancer, and prognostic for a particular cancer. We have discovered and sequenced MSH5 in this in a number of mammals, including humans. This gene can be used in assays, to express gene product, for drug screens, and therapeutically. | 08-21-2008 |
| 20080199877 | OLIGONUCLEOTIDE PROBES FOR DETECTING ENTEROBACTERIACEAE AND QUINOLONE-RESISTANT ENTEROBACTERIACEAE - Oligonucleotide probes for detecting Enterobacteriaceae species. Unique gyrA coding regions permit the development of probes specific for eight different species: | 08-21-2008 |
| 20080199878 | DETECTION, IDENTIFICATION AND DIFFERENTIATION OF EUBACTERIAL TAXA USING A HYBRIDIZATION ASSAY - The present invention relates to a method for the specific detection and/or identification of | 08-21-2008 |
| 20080206743 | Rapid and Specific Detection of Enterobacter Sakazakii - The present invention provides a method for specifically detecting pathogenic | 08-28-2008 |
| 20080206744 | Functional Genomics and Gene Trapping in Haploid or Hypodiploid Cells - The present invention provides methods and compositions for performing functional genomics and gene trapping using haploid cells, including haploid or hypodiploid vertebrate cells. The present invention further provides methods for identifying genes involved in cellular signaling pathways. | 08-28-2008 |
| 20080206745 | Nucleic acid extraction solution and use thereof - Disclosed are methods and compositions for extracting nucleic acids from a biological sample. In particular, disclosed is a nucleic acid extraction solution together with methods using such a solution for extracting nucleic acid sequences from biological samples containing cells, cellular debris or both. The nucleic acid extraction solution contains a molecule having the formula R | 08-28-2008 |
| 20080206746 | Plasmid DNA isolation - Apparatus, reagents, and methods for isolating plasmid DNA from bacteria by alkaline lysis using a solid or immobilized P | 08-28-2008 |
| 20080206747 | Methods, Kits and Compositions Pertaining to Combination Oligomers and Libraries for Their Preparation - This invention pertains to the field of combination oligomers, including the block synthesis of combination oligomers in the absence of a template, as well as related methods, kits, libraries and other compositions. | 08-28-2008 |
| 20080206748 | Methods for genetic analysis of DNA to detect sequence variances - Methods for determining genotypes and haplotypes of genes are described. Also described are single nucleotide polymorphisms and haplotypes in the ApoE gene and methods of using that information. | 08-28-2008 |
| 20080206749 | Methods and kits for diagnosis, prognosis or monitoring of Epstein-Barr virus (EBV)-associated cancer - Disclosed is a non-invasive method for diagnosis, prognosis or monitoring of Epstein-Barr virus (EBV)-associated cancer by detecting and/or quantifying EBV associated nucleic acid fragments in a urine sample from an individual. Kits for diagnosis, prognosis or monitoring of cancer are also disclosed. | 08-28-2008 |
| 20080206750 | Novel fetal genes - Novel fetal genes (fls353 and fls485) have been successfully isolated from human fetal liver-derived cDNAs. These genes were specifically expressed in tissues including fetal tissues which are thought to contain a large number of undifferentiated cells and actively differentiating/proliferating cells. High levels of expression of these genes were observed also in a variety of cancer cells. The proteins and genes encoding the proteins can be used as the tool for developing drugs for the treatment of tumors. | 08-28-2008 |
| 20080206751 | Method For Carrying Out A Multi-Step Reaction, Breakable Container For Storing Reagents And Method For Transferring Solid Reagent Using An Electrostatically Charged Wand - The application relates to a method of performing a multi-step reaction vessel ( | 08-28-2008 |
| 20080206752 | Method For the Photochemical Attachment of Biomolecules to a Substrate - Methods and devices for attaching biomolecules to a solid substrate surface for example to the inner surface of a capillary. In particular, the invention relates to compounds and methods for creating patterned arrays of biomolecules inside fused silica capillaries so that a plurality of bioassays can be conducted simultaneously. | 08-28-2008 |
| 20080206753 | Methods for Cancer Prognosis - A method for assessing prognosis in a subject having a breast tumor comprises determining the level of expression of at least one Notch receptor gene, Notch ligand gene or Notch signaling target gene. A method of treating a subject suffering from a breast tumor associated with increased Notch signaling comprises administering to the subject an effective amount of an inhibitor of Notch signaling. | 08-28-2008 |
| 20080206754 | Method for producing monoclonal antibodies - An improved method for the production of monoclonal antibodies is disclosed. | 08-28-2008 |
| 20080206755 | Method for genetic detection using interspersed genetic elements - The way to design a “filled” site (which contains an interspersed element) primer set to target a particular locus is to design one of the two primers such that it encompasses that unique information (e.g., interspersed element+flanking genomic sequence+direct repeat). The way to design an “empty” site primer is to design one of the two primers such that the entire direct repeat sequence in addition to flanking genomic sequence is included on both sides. To improve efficiency, the “empty” site primer designed around the direct repeat should not be too long. This primer design of the present invention allows for the ability to test any type of interspersed genetic element containing characteristic direct repeat sequences (direct repeats). This gives the option of many new polymorphic marker sites because Alu elements are not the only interspersed genetic elements having direct repeats flanking their core sequence. | 08-28-2008 |
| 20080206756 | Biomarker panel for colorectal cancer - A panel of biomarkers has been identified for analysis of colorectal cancer. The panel, originally identified using a mouse colon cancer model, has been used to assess changes in human tissue from surgical and biopsy samples against a normal human control panel of biomarkers. The panel may be used for providing a cost effective, rapid, noninvasive procedure for risk assessment, early diagnosis, establishing prognosis, monitoring patient treatment, detecting relapse, and for the discovery of therapeutic intervention of colorectal cancer. | 08-28-2008 |
| 20080206757 | METHODS AND COMPOSITIONS FOR DETECTING RARE CELLS FROM A BIOLOGICAL SAMPLE - The present invention provides methods and compositions for isolating and detecting rare cells from a biological sample containing other types of cells. In particular, the present invention includes a debulking step that uses a microfabricated filters for filtering fluid samples and the enriched rare cells can be used in a downstream process such as identifies, characterizes or even grown in culture or used in other ways. The invention also include a method of determining the aggressiveness of the tumor or of the number or proportion of cancer cells in the enriched sample by detecting the presence or amount of telomerase activity or telomerase nucleic acid or telomerase expression after enrichment of rare cells. This invention further provides an efficient and rapid method to specifically remove red blood cells as well as white blood cells from a biological sample containing at least one of each of red blood cells and white blood cells, resulting in the enrichment of rare target cells including circulating tumor cells (CTC), stromal cells, mesenchymal cells, endothelial cells, fetal cells, stem cells, non-hematopoietic cells etc from a blood sample. The method is based upon combination of immuno-microparticles (antibody coated microparticles) and density-based separation. The final enriched target cells can be subjected to a variety of analysis and manipulations, such as flowcytometry, PCR, immunofluorescence, immunocytochemistry, image analysis, enzymatic assays, gene expression profiling analysis, efficacy tests of therapeutics, culturing of enriched rare cells, and therapeutic use of enriched rare cells. In addition, depleted plasma protein and white blood cells can be optionally recovered, and subjected to other analysis such as inflammation studies, gene expression profiling, etc. | 08-28-2008 |
| 20080206758 | POLYNUCLEIC ACID-ATTACHED PARTICLES AND THEIR USE IN GENOMIC ANALYSIS - Disclosed are methods for preparing particle-linked polynucleotides, and using the particle linked polynucleotides in genomic analysis. The particles as disclosed are characterized as having a size variance of less than 2%. | 08-28-2008 |
| 20080206759 | Gene regulatory networks and methods of interdiction for controlling the differentiation state of a cell - The invention provides a method of modulating a regulatory state of a cell. The method consists of: (a) identifying a point of interdiction within a cis regulatory network specifying a genetic regulatory architecture of a cell, and (b) introducing into a progenitor cell two or more network elements within said network to induce a predetermined series of cis regulatory network interactions resulting in a specified regulatory state of said progenitor cell. Also provided is a method of modulating a regulatory state of a cell. The method consists of: (a) identifying a point of interdiction within a cis regulatory network specifying a genetic regulatory architecture of a cellular state, and (b) introducing into a progenitor cell two or more network elements within said network to induce a predetermined series of cis regulatory network interactions resulting in a specified regulatory state of said progenitor cell. A cell having a specified regulatory state consisting of a modified genetic regulatory architecture is further provided. Methods of diagnosing and methods of treating an individual suffering from a cellular defect also are provided. The invention additionally provides a method of identifying a compound having differentiation or cell fate inducing activity. | 08-28-2008 |
| 20080206760 | Method for detecting DNA methylation using labelled S-adenosylmethionine analogs - The invention relates to a method for detecting the methylation status in DNA samples. According to the invention, a DNA methyl transferase and a labeled S-adenosylmethionine derivative allow a detectable label to be covalently bonded to the DNA, in accordance with the respective methylation status of the DNA sample. | 08-28-2008 |
| 20080206761 | Ex Vivo Gene Expression in Whole Blood as a Model of Assessment of Individual Variation to Dietary Supplements - A method is disclosed for individually tailoring the administration of dietary components such as supplements. In the method, whole blood of a mammal is exposed to a dietary component. The level of a marker mRNA linked to a disease state is measured in leukocytes after exposure to the dietary component, and in some cases after further stimulation of the exposed blood cells. By comparing the mRNA level after exposure with the value found in unexposed blood cells, it is possible to determine what the effect of the dietary component will be in the mammal. By screening blood of the mammal against a number of possible dietary components, it is possible to develop an optimized set of dietary components tailored to the specific mammal to treat or prevent a disease state. | 08-28-2008 |
| 20080206762 | Method for the Diagnosis of Alzeimer's Disease - The invention relates to a method for the diagnosis and/or prognosis of Alzheimer's disease, consisting in determining the expression level of a gene encoding a lysosomal marker. | 08-28-2008 |
| 20080206763 | METHODS FOR ISOLATING AND CHARACTERIZING ENDOGENOUS mRNA-PROTEIN (mRNP) COMPLEXES - Cellular mRNA-protein (mRNP) complexes are partitioned in vivo by contacting a biological sample with at least one ligand that specifically binds at least one component of a mRNP complex. Suitable biological samples comprise at least one mRNA-protein (mRNP) complex and include cell cultures, cell extracts, and whole tissue, including tumor tissue. Ligands include antibodies that specifically bind RNA-binding or RNA-associated proteins present in the mRNP complex. The mRNP complex is separated by binding the ligand with a binding molecule specific for the ligand, where the binding molecule is attached to a solid support. The mRNP complex is collected by removing the mRNP complex from the solid support. After collecting the mRNP complex, the mRNA bound within the complex may be characterized and identified. Subsets of the total mRNA population of a cell may accordingly be characterized, and a gene expression profile of the cell obtained. | 08-28-2008 |
| 20080206764 | Flowcell system for single molecule detection - The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex. | 08-28-2008 |
| 20080206765 | MN Gene and Protein - Identified herein is the location of the MN protein binding site, and MN proteins/polypeptides that compete for attachment to vertebrate cells with immobilized MN protein. Such MN proteins/polypeptides prevent cell-cell adhesion and the formation of intercellular contacts. The MN protein binding site is a therapeutic target that can be blocked by organic or inorganic molecules, preferably organic molecules, more preferably proteins/polypeptides that specifically bind to that site. Therapeutic methods for inhibiting the growth of preneoplastic/neoplastic vertebrate cells that abnormally express MN protein are disclosed. Vectors are provided that encode the variable domains of MN-specific antibodies and a flexible linker polypeptide separating those domains. Further vectors are disclosed that encode a cytotoxic protein/polypeptide operatively linked to the MN gene promoter or a MN gene promoter fragment comprising the HIF-1 consensus binding sequence, and which vectors preferably further encode a cytokine. The MN gene promoter is characterized, and the binding site for a repressor of MN transcription is disclosed. Further, the hypoxia inducibility of the MN gene and the uses of such inducibility are disclosed. | 08-28-2008 |
| 20080206766 | Compositions and Methods for Detecting And Treating Prostate Disorders - The present invention relates to compositions and methods for the detecting, treating, and empirically investigating cellular proliferation disorders and cellular motility disorders. In particular, the present invention provides compositions and methods for using CXCL chemokines (e.g., CXCL1, CXCL5, CXCL6, CXCL12), CXCL receptors (e.g., CXCR1, CXCR2, CXCR4, CXCR7), and/or pathway related compounds (e.g., NF-kappaB, ERK ½, ELK-1) in the diagnosis, treatment, and empirical investigation of prostate disorders (e.g., prostate cancer, benign prostatic hypertrophy, prostatitis). | 08-28-2008 |
| 20080206767 | System and method for tracking and controlling infections - The present invention is a system and method for performing real-time infection control over a computer network. The method comprises obtaining a sample of a microorganism at a health care facility, sequencing a first region of a nucleic acid from the microorganism sample, comparing the first sequenced region with historical sequence data stored in a database, determining a measure of phylogenetic relatedness between the microorganism sample and historical samples stored in the database, and providing infection control information based on the phylogenetic relatedness determination to the health care facility, thereby allowing the health care facility to use the infection control information to control or prevent the spread of an infection. | 08-28-2008 |
| 20080206768 | Predicting a response to olanzapine - The invention relates generally to the relative effect of specific genetic polymorphisms in predicting the clinical outcome of olanzapine therapy in patients suffering from a psychiatric disease such as schizophrenia. | 08-28-2008 |
| 20080206769 | Molecular prognostic signature for predicting breast cancer distant metastasis, and uses thereof - The present invention is based on the discovery of a unique 14-gene molecular prognostic signature that is useful for predicting breast cancer metastasis. In particular, the present invention relates to methods and reagents for detecting and profiling the expression levels of these genes, and methods of using the expression level information in predicting risk of breast cancer metastasis. | 08-28-2008 |
| 20080206770 | SCREENING METHODS USED TO IDENTIFY COMPOUNDS THAT MODULATE SKIN STROMAL CELLS (FIBROBLASTS) ABILITY TO MODIFY FUNCTION OF EXTRACELLULAR MATRIX - The cellular response to cosmetic products has been characterized on the molecular level through the use of gene and protein expression technologies. Nucleic acid and protein molecules, the expression of which are induced or repressed in response to exposure to cosmetics, are identified according to a temporal pattern of altered expression post exposure. Methods are disclosed that utilized these cosmetics-regulated molecules as markers for effectiveness of cosmetics. Other screening methods of the invention are designed for the identification of compounds that modulate the response of a cell to exposure to cosmetics. The invention also provides compositions useful for drug screening or pharmaceutical purposes. | 08-28-2008 |
| 20080206771 | Method For Recovering Nucleic Acid From A Mixed Cell Suspension, Without Centrifugation - A method for selectively recovering nucleic acid from a first cell type in a sample containing cells of at least a first cell type and a second cell type, and a cell suspension medium comprising extracellular impurities, is provided. The method entails combining the sample with particles responsive to a magnetic field in a vessel, the magnetic particles having the ability to sequester the cells from the cell suspension medium upon application of a magnetic field; exposing the vessel to a magnetic field for a time sufficient to cause sequestration of the cells by the particles; removing the impurities-containing cell suspension medium from the vessel while retaining the cells; lysing selectively cells of the first cell type; and isolating the nucleic acid from the lysed cells. Methods for recovering nucleic acid from the second cell type are also provided. | 08-28-2008 |
| 20080206772 | SAMPLE TREATMENT SOLUTION AND REAGENT KIT FOR PREPARING SAMPLE FOR DETECTING DNA METHYLATION - The present invention provides a sample treatment solution for preparing a sample for DNA methylation which can achieve stable detection results in detection of DNA methylation and be easily pretreated, comprising an aqueous solution containing a protease. | 08-28-2008 |
| 20080206773 | DNA Fingerprinting Using Allelic Specific Oligonucleotide Reversed DOT BLOT (ASO-RDB) Flow Through Hybridization Process and Device - The present invention disclosed the use of single nucleotide polymorphism (SNP) as the detection assay for human identification. Using the reversed dot-blot format and the flow through hybridization process, the process can be more efficient, less expensive and with similar or better power of exclusion in definitive identification. The present method can be applied to any other organisms. | 08-28-2008 |
| 20080206774 | AUTOMATED CANCER DIAGNOSTIC METHODS USING FISH - In various embodiments methods for automated screening for gene amplification in biological tissue samples using an automated fluorescence microscope to analyze fluorescence in situ hybridized samples are provided. Various additional embodiments provide methods of high throughput screening for gene amplification. | 08-28-2008 |
| 20080206775 | Method of Amplifying Nucleic Acids, Reagent Kit for Amplifying Nucleic Acids, Method of Detecting Single Nucleotide Polymorphism, and Reagent Kit for Detecting Single Nucleotide Polymorphism - An object of the present invention is to provide a nucleic acid amplification method for amplifying a desired nucleic acid while suppressing amplification of byproducts in a PCR reaction, a reagent kit used for nucleic acid amplification, a method of detecting single nucleotide polymorphism to detect single nucleotide polymorphism by utilizing that amplification of byproducts is suppressed in a PCR reaction, and a reagent kit used for detecting single nucleotide polymorphism. The method of amplifying nucleic acids by PCR is characterized by admixing in a reaction solution, a homologous recombinant protein which contains at least one of a RecA protein derived from | 08-28-2008 |
| 20080206776 | Use of Both Rd9 and Is6110 as Nucleic Acid Targets for the Diagnosis of Tuberculosis, and Provision of Multiplex-Compliant Is6110 and Rd9 Targets - The present invention relates to the use of both RD9 and IS6110 as nucleic acid targets, for the specific and sensitive detection of a mycobacterium of the | 08-28-2008 |
| 20080206777 | Gene and protein expression profiles associated with the therapeutic efficacy of EGFR-TK inhibitors - The present invention provides protein and gene expression profiles indicative of whether a patient afflicted with non-small cell lung cancer is likely to be responsive to treatment with a therapeutic compound that is a EGFR-TK inhibitor. By identifying such responsiveness, a treatment provider may determine in advance those patients who would benefit from such treatment, as well as identify alternative therapies for non-responders. The present invention further provide methods of using the gene and protein expression profiles, and assays for identifying the presence of a gene or protein expression profile in a patient sample. | 08-28-2008 |
| 20080206778 | Feline Hemoplasma Isolate - A newly identified hemoplasma agent, Candidatus | 08-28-2008 |
| 20080206779 | Methods and Kits for Multiplex Hybridization Assays - The invention provides a method for genotyping interfering polymorphic loci in a target polynucleotide, such as a strand of genomic DNA, in a multiplex hybridization-based assay. The invention also provides nucleic acid standards for validating the performance of such hybridization-based assays. In one aspect, the method of the invention is carried out by providing for each interfering polymorphic locus one or more probes so that at least one probe is capable of forming a perfectly match duplex at the locus regardless of the characteristic sequence of an adjacent polymorphism. | 08-28-2008 |
| 20080213754 | Method of Amplifying Atp and Use Thereof - The ATP amplification method of the present invention is a method for amplifying and detecting a very trace amount of exogenous ATP by allowing a fusion protein (PPK-ADK) of a polyphosphate kinase and an adenylate kinase, the fusion protein not containing ADP, to act on a mixture of ATP, AMP, and a polyphosphate compound. The present invention also provides an ultrasensitive ATP amplification method by which ATP at a single cell level can be amplified and detected, and an ultrasensitive microbial assay based on this ATP amplification method. | 09-04-2008 |
| 20080213755 | Method and Device for Detection of Nucleic Acid Sequences | 09-04-2008 |
| 20080213756 | Real-Time Pcr Point Mutation Assays For Detecting Hiv - 1 Resistance to Antiviral Drugs - Disclosed are compositions including primers and probes, which are capable of interacting with the disclosed nucleic acids, such as the nucleic acids encoding the reverse transcriptase or protease of HIV as disclosed herein. Thus, provided is an oligonucleotide comprising any one of the nucleotide sequences set for in SEQ ID NOS:1-89, and 96-104. Also provided are the oligonucleotides consisting of the nucleotides as set forth in SEQ ID NOS: 1-89, and 96-104. Each of the disclosed oligonucleotides is a probe or a primer. Also provided are mixtures of primers and probes and for use in RT-PCR and primary PCR reactions disclosed herein. Provided are methods for the specific detection of several mutations in HIV. Mutations in both the reverse transcriptase and the protease of HIV can be detected using the methods described herein. | 09-04-2008 |
| 20080213757 | Methods of Distinguishing Types of Spinal Neurons Using Corl1 Gene as an Indicator - As a result of screening for genes that are selectively expressed in fetal mouse brain region by subtraction method, the present inventors obtained a cDNA fragment encoding Corl1. The expression of Corl1 was examined by RT-PCR, in situ hybridization, and immunostaining using polyclonal antibodies. The results demonstrated that Corl1 was especially expressed at a high level of selectively in the central nervous system during embryonic stages. The expression patterns of Corl1 determined using various markers in embryonic spinal cord were compared to identify types of neurons expressing Corl1. The results revealed that Corl1 was specifically expressed in spinal cord interneurons dI4, dI5, dILA, and dILB. Accordingly, the present invention provides for discrimination between dI4 and dI6, neurons which previously could only be discriminated based on developmental location, using the expression of Corl1 as an indicator. | 09-04-2008 |
| 20080213758 | Method For Estimating End Use Qualities of Wheat at Growth Stage - The invention provides a means for estimating the end use qualities of wheat flour that will be obtained in the future from the harvested wheat at an early stage before maturation of the wheat seeds. The invention relates to a method for estimating the end use qualities of a matured wheat seed, comprising measuring the expression level of at least 1 gene selected from genes, each of which is defined by any one of the nucleotide sequences of SEQ ID NOS: 1 to 121 in immature wheat. | 09-04-2008 |
| 20080213759 | Novel Angiogenesis Inhibitor - Because AK022567 has angiogenesis inhibitory activity, it is useful as an angiogenesis inhibitor. Furthermore, 4 splicing variants obtained from the same gene are also useful as an angiogenesis inhibitor. These 5 polypeptides, polynucleotides encoding the polypeptides and antibodies against the polypeptides are useful for screening of a candidate compound as an angiogenesis inhibitor or promoter. A compound obtained from the screening is useful as a medicine and can be used for a preventive or therapeutic agent for an angiogenesis related disease. | 09-04-2008 |
| 20080213760 | COMPOSITIONS AND METHODS FOR PROTEIN ISOLATION - The invention provides for polynucleotides and vectors comprising at least two tag sequences. The invention also provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for polynucleotides and vectors wherein a gene of interest is fused in frame to at least two tag sequences, for example, a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for the chimeric proteins encoded by these polynucleotides. The invention also provides for methods of using the polynucleotides of the invention for detecting and/isolating protein complexes or identifying a binding partner for a protein of interest. | 09-04-2008 |
| 20080213761 | Agonists of Bitter Taste Receptors and Uses Thereof - The present invention relates to agonists of the hTAS2R1, hTAS2R3, hTAS2R7 and hTAS2R40 bitter taste receptors, respectively, and their role in bitter taste transduction. The invention also relates to assays for screening molecules that modulate, e.g. suppress or block hTAS2R1, hTAS2R3, hTAS2R7 or hTAS2R40 bitter taste transduction or bitter taste response. | 09-04-2008 |
| 20080213762 | Method of Gene Sequence Examination - The present invention provides a method for detecting quantitatively a specific nucleic acid sequence and for detecting gene polymorphism or mutation by homogenous system simply, promptly, accurately, and inexpensively, and an oligonucleotide probe used therefor. The nucleic acid having a specific sequence is quantitatively detected and gene polymorphism or mutation is detected in such a way that a probe DNA containing one or two labeling materials is hybridized to the target nucleic acid, and decomposed from the 5′- or the 3′-terminal by exonuclease action to release one of the labeling materials, the signal emitted by which is then detected. | 09-04-2008 |
| 20080213763 | Human Protooncogene and Protein Encoded by Same, and Expression Vector Containing Same - Disclosed are a novel protooncogene and a protein encoded by same. The protooncogene of the present invention is a novel gene, and may be effectively used for diagnosing the cancers, including leukemia, uterine cancer, lymphoma, colon cancer, lung cancer, skin cancer, etc., as well as producing transformed animals, etc. | 09-04-2008 |
| 20080213764 | Human Protooncogene and Protein Encoded Therein - Disclosed are a novel protooncogene and a protein encoded therein. The protooncogene of the present invention, which is a novel gene that takes part in human carcinogenesis and simultaneously has an ability to induce cancer metastasis, may be effectively used for diagnosing the cancers, including lung cancer, leukemia, uterine cancer, lymphoma, colon cancer, skin cancer, etc., as well as producing transformed animals, etc. | 09-04-2008 |
| 20080213765 | Human Autism Susceptibility Genes Encoding a Neurotransmitter Transporter and Uses Thereof - The present invention discloses the identification of a human autism susceptibility gene, which can be used for the diagnosis, prevention and treatment of autism and related disorders, as well as for the screening of therapeutically active drugs. The invention more specifically discloses that the SLC6A1 or SLC6A11 gene on chromosome 3 and certain alleles thereof are related to susceptibility to autism and represent novel targets for therapeutic intervention. The present invention relates to particular mutations in the SLC6A1 or SLC6A11 gene and expression products, as well as to diagnostic tools and kits based on these mutations. The invention can be used in the diagnosis of predisposition to, detection, prevention and/or treatment of Asperger syndrome, pervasive developmental disorder, childhood disintegrative disorder, mental retardation, anxiety, depression, attention deficit hyperactivity disorders, speech delay, epilepsy, metabolic disorder, immune disorder, bipolar disease and other psychiatric and neurological diseases including schizophrenia. | 09-04-2008 |
| 20080213766 | METHOD AND DEVICE FOR DETECTING THE PRESENCE OF A SINGLE TARGET NUCLEIC ACID IN SAMPLES - A method comprising for each individual sample of a plurality of samples, loading at least one sample portion of the individual sample into at least one respective sample chamber of a plurality of sample chambers, subjecting the sample portions to at least a first amplification step; and then determining whether sample portions contain at least one molecule of the target nucleic acid. For each sample portion, if the sample portion contains at least a single molecule of the target nucleic acid, the sample portion would attain a detectable concentration of the target nucleic acid after a single round of amplification. | 09-04-2008 |
| 20080213767 | Detection Of Nucleic Acids By Target-Catalyzed Product Formation - A method is disclosed for modifying an oligonucleotide, which method has application to the detection of a polynucleotide analyte. An oligonucleotide is reversibly hybridized with a polynucleotide, for example, a polynucleotide analyte, in the presence of a 5′-nuclease under isothermal conditions. The polynucleotide analyte serves as a recognition element to enable a 5′-nuclease to cleave the oligonucleotide to provide (i) a first fragment that is substantially non-hybridizable to the polynucleotide analyte and (ii) a second fragment that lies 3′ of the first fragment (in the intact oligonucleotide) and is substantially hybridizable to the polynucleotide analyte. At least a 100-fold molar excess of the first fragment and/or the second fragment are obtained relative to the molar amount of the polynucleotide analyte. The presence of the first fragment and/or the second fragment is detected, the presence thereof indicating the presence of the polynucleotide analyte. The method has particular application to the detection of a polynucleotide analyte such as DNA. Kits for conducting methods in accordance with the present invention are also disclosed. | 09-04-2008 |
| 20080213768 | Identification and use of biomarkers for non-invasive and early detection of liver injury - The present invention provides methods for identifying and evaluating suites of biochemical and/or gene entities useful as biomarkers for early prediction of disease and/or toxicity, disease staging, target identification/validation, and monitoring of drug efficacy/toxicity. The present invention further provides suites of small molecule entities as biomarkers for non-invasive and early prediction of hepatic injury. | 09-04-2008 |
| 20080213769 | Automated Method for Detecting Cancers and High Grade Hyperplasias - Automated methods for detecting cancer and related hyperplasias in biological samples. | 09-04-2008 |
| 20080213770 | Method of Determining The Nucleotide Sequence of Oligonucleotides and DNA Molecules - The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions. | 09-04-2008 |
| 20080213771 | Methods and Compositions for Large-Scale Analysis of Nucleic Acids Using DNA Deletions - The present invention is related generally to analysis of polynucleotides, particularly polynucleotides derived from genomic DNA. The invention provides methods, compositions and systems for such analysis. Encompassed by the invention are constructs that include pairs of target sequences which are separated by a known distance in the polynucleotide from which they are derived. | 09-04-2008 |
| 20080213772 | USE OF MICROPHTHALMIA FOR DIAGNOSIS, PROGNOSIS AND/OR TREATMENT OF MELANOMA - Microphthalmia (Mi) while present in melanocytes, a cells and osteoclast, is not normally present in other cells. We have found that Mi is present in the nucleus of melanoma cells. Melanoma can be diagnosed by contacting a malignant cell with a probe for Mi. If the probe identities Mi in the nucleus of the cell, the cell is a melanoma. | 09-04-2008 |
| 20080213773 | Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing - A method for identifying an RNA form of a bacteria, comprising reverse transcribing RNA material; conducting PCR using primers for a first highly conserved genetic sequence generic of the bacteria; conducting nested PCR using primers for a second highly conserved genetic sequence within the first genetic sequence of the bacteria; and identifying the bacteria based on unconserved amplified sequences linked to the conserved sequences. | 09-04-2008 |
| 20080213774 | Mutations in Kit Confer Imatinib Resistance in Gastrointestinal Stromal Tumors - The present invention relates to methods and compositions concerning resistance to a drug for cancer comprising aberrant KIT signal, such as aberrant KIT sequence or expression. In a specific embodiment, the cancer is also initially responsive to imatinib therapy, such as in gastrointestinal stromal tumors (GISTs). In particular embodiments, a mutation in a KIT polynucleotide confers resistance to imatinib treatment, and in specific embodiments the exemplary mutation is at 1982T→C. Thus, the invention provides a means to adjust for or circumvent the resistance to imatinib drug treatment. | 09-04-2008 |
| 20080213775 | METHODS AND MATERIALS FOR IDENTIFYING POLYMORPHIC VARIANTS, DIAGNOSING SUSCEPTIBILITIES, AND TREATING DISEASE - The invention is directed to materials and methods associated with polymorphic variants in two enzymes involved in folate-dependent and one-carbon metabolic pathways: MTHFD1 (5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase) and methylenetetrahydrofolate dehydrogenase (NADP+dependent) 1-like (MTHFD1L). Diagnostic and therapeutic methods are provided involving the correlation of polymorphic variants in MTBFD1, MTHFD1, and other genes with relative susceptibility for various pregnancy-related and other complications. | 09-04-2008 |
| 20080213776 | METHOD FOR TREATING AUTOIMMUNE DISEASES AND SCREENING METHOD FOR PREVENTIVE OR THERAPEUTIC AGENT FOR THE SAME - The present invention is directed to a method for treating autoimmune diseases comprising administering RBAp48 production suppressor or inhibitor; a screening method for a preventive or therapeutic agent for autoimmune diseases comprising determining RBAp48 production suppressing effect or inhibitory effect of a sample; a diagnosis agent or a diagnosing kit for autoimmune diseases containing a reagent for measuring RBAp48 level in gland tissue; and a diagnosis method for autoimmune diseases comprising measuring RBAp48 level in gland tissue. | 09-04-2008 |
| 20080213777 | Tumor suppressor pathway in C. elegans - The invention provides novel lin-8, lin-56, and lin-61 genes and polypeptides involved in cell fate determination and in cell proliferation. In addition, the invention includes mutants of these three genes, as well as methods for utilizing these genes, and their encoded polypeptides, in diagnosing and treating abnormal cell proliferation. | 09-04-2008 |
| 20080213778 | Novel genes encoding proteins having prognostic, diagnostic, preventive, therapeutic, and other uses - The invention provides isolated TANGO 239, TANGO 219, TANGO 232, TANGO 281, A236 (INTERCEPT 236), TANGO 300, TANGO 353, TANGO 393, TANGO 402, TANGO 351 and TANGO 509 nucleic acid molecules and polypeptide molecules. The invention also provides antisense nucleic acid molecules, expression vectors containing the nucleic acid molecules of the invention, host cells into which the expression vectors have been introduced, and non-human transgenic animals in which a nucleic acid molecule of the invention has been introduced or disrupted. The invention still further provides isolated polypeptides, fusion polypeptides, antigenic peptides and antibodies. Diagnostic, screening and therapeutic methods utilizing compositions of the invention are also provided. | 09-04-2008 |
| 20080213779 | Nalp7-Based Diagnosis of Female Reproductive Conditions - Methods, reagents and kits are described for the diagnosis of a female reproductive condition, based on the detection of an alteration in a NALP7-encoding nucleic acid or a NALP7 polypeptide, relative to a corresponding wild-type NALP7-encoding nucleic acid or NALP7 polypeptide. | 09-04-2008 |
| 20080213780 | MULTI-COLOR TIME RESOLVED FLUOROPHORES BASED ON MACROCYCLIC LANTHANIDE COMPLEXES - The present invention provides a novel class of macrocyclic compounds as well as complexes formed between a metal (e.g., lanthanide) ion and the compounds of the invention. Preferred complexes exhibit high stability as well as high quantum yields of lanthanide ion luminescence in aqueous media without the need for secondary activating agents. Preferred compounds incorporate hydroxy-isophthalamide moieties within their macrocyclic structure and are characterized by surprisingly low, non-specific binding to a variety of polypeptides such as antibodies and proteins as well as high kinetic stability. These characteristics distinguish them from known, open-structured ligands. | 09-04-2008 |
| 20080213781 | Methods of detecting methylation patterns within a CpG island - A method of increasing sensitivity of a DNA methylation assay by determining complementation within a CpG island of the methylated DNA. | 09-04-2008 |
| 20080213782 | SOCS-1 Gene Methylation in Cancer - Methods are provided for identifying a cell exhibiting unregulated growth associated with methylation-silenced transcription of a suppressor of cytokine signaling (SOCS)/cytokine-inducible SH2 protein (CIS) family member (SOCS/CIS) gene such as the SOCS-1 gene. In addition, methods of treating a cancer patient, wherein cancer cells in the patient exhibit methylation-silenced transcription of SOCS/CIS gene such as a SOCS-1 gene, are provided, as are reagents for practicing such methods. | 09-04-2008 |
| 20080213783 | SITE-SPECIFIC ENZYMATIC DEPOSITION OF METAL IN SITU - The present invention provides compositions, kits, assembles of articles and methodology for carrying out processes that permit biological enzymes to act directly on metals and metal particles. In particular, the invention relates to use of enzymes to selectively deposit metal to the vicinity of a target molecule. The invention also relates to linking of metals to enzyme substrates, control of enzymatic metal deposition and applications of enzymatic metal deposition to sensitively and selectively detect target molecules such as biomarkers in various biological samples, such as chromogenic immunohistochemical (IHC) detection in situ by using bright field light microscope. | 09-04-2008 |
| 20080213784 | SCREENING METHODS AND SEQUENCES RELATING THERETO - A screening method for identifying an individual having a pre-disposition towards having a cancer is disclosed, which screening method comprises the steps of:
| 09-04-2008 |
| 20080213785 | Method of predicting a benefit of antioxidant therapy for prevention or treatment of vasclar disease in hyperglycemic individuals - This invention relates to methods and compositions of determining the benefit of therapy using antioxidant for the treatment of cardiovascular events in individuals with diabetes mellitus based on their haptoglobin phenotype and the treatment of the cardiovascular events using antioxidants based on the haptoglobin phenotype. | 09-04-2008 |
| 20080213786 | Treatment of rheumatoid arthritis with galectin-3 antagonists - The invention encompasses a novel method of treating an inflammatory disease, such as rheumatoid arthritis, and novel methods of identifying and screening for drugs useful in the treatment of inflammatory diseases, such as rheumatoid arthritis, and their clinical symptoms. The inventors have made the discovery that the activity of galectin-3, a β-galactoside-binding lectin known to have an effect on some cancers, has a significant impact on the pathophysiology of rheumatoid arthritis. The symptoms of an inflammatory disease, such as rheumatoid arthritis, may be alleviated by administering a compound that inhibits the activity of galectin-3. | 09-04-2008 |
| 20080213787 | Methods and Compositions for Kir Genotyping - The present invention provides methods for single nucleotide polymorphism (SNP)-based killer cell immunoglobulin-like receptor (KIR) gene cluster genotyping using the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer. In general, the methods involve amplifying a plurality of target sequences of a plurality of KIR genes, and detecting the presence or absence of a plurality of single SNPs of the plurality of KIR genes by MALDI-TOF mass spectrometry. The invention also features compositions, including arrays of capture primers and optionally extension primers on a substrate surface, and kits, for use in the methods of the invention. | 09-04-2008 |
| 20080213788 | GENE CODING FOR SCYTALONE DEHYDRATASE EXHIBITING RESISTANCE TO AGRICULTURAL FUNGICIDAL AGENT - The present invention provides a gene that can be used extensively in studies relating to resistant rice blast fungi. | 09-04-2008 |
| 20080213789 | Assay for detecting methylation status by methylation specific primer extension (MSPE) - The present invention relates to detecting the relative methylation levels at one or more CpG sites on a nucleic acid molecule, by using the methylation specific primer extension reaction (MSPE). MSPE uses an agent to modify unmethylated cytosine at a CpG site to uracil and subsequently amplify the chemically treated nucleic acids. The MSPE primers distinguishing between unmethylated and methylated CpG sites are provided to conduct MSPE. Relative methylation levels at one or more CpG sites are performed by detecting the signal intensity of labels incorporated into the MSPE reaction products. | 09-04-2008 |
| 20080213790 | METHOD FOR SYNTHESIZING POLYNUCLEOTIDES - The present invention realized isothermal and rapid polynucleotide synthesis by using as templates polynucleotides having a structure capable of forming loops, and combining a plurality of primers capable of providing a starting point for complementary strand synthesis to such loops. If the LAMP method is applied, all reactions can be carried out isothermally and rapidly since the template polynucleotides themselves can also be synthesized by an isothermal reaction. | 09-04-2008 |
| 20080213791 | Materials and methods for assaying for methylation of CpG islands associated with genes in the evaluation of cancer - Provided are methods, reagents, and kits for evaluating cancer, such as prostate cancer, in a subject. Disclosed methods of evaluating cancer include methods of diagnosing cancer, methods of prognosticating cancer and methods of assessing the efficacy of cancer treatment. The methods include assaying a biological sample for methylation of a CpG island associated with specified genes. Provided reagents and kits include primers suitable for amplifying at least a portion of a target CpG islands associated with specified genes. | 09-04-2008 |
| 20080213792 | Homogeneous Multiplex Screening Kits - Kits for highly multiplexed homogeneous in vitro screening assays for numerous possible nucleic acid targets, any of which might be present in a sample, that utilize fluorescent hybridization probes that are combinatorially coded from a panel of fluorophores by subdividing each probe into portions and differently labeling each portion such that, when portions are combined, each probe has a unique code. The kits may include reagents and primers for target amplification and real-time detection. | 09-04-2008 |
| 20080220413 | Clean-Up Beads - The present invention provides a material for separating an analyte from an undesired constituent, which material comprises a solid phase and a coating, wherein the solid phase is capable of binding the undesired constituent, and wherein the coating covers the exposed surface of the solid phase to an extent that any binding of the solid phase to the analyte is impeded. A method for preparing the material, and uses of the material for separating an analyte from an undesired constituent are also provided. | 09-11-2008 |
| 20080220414 | Method, Chip, Device and Integrated System for Detection Biological Particles - The present invention relates to a method, a chip, a device, and a system for detection of biological particles. The method of the invention typically comprises collecting the biological particles from a gaseous sample, contacting the biological particles with a first liquid reagent, extracting biological material from the collected biological particles, and analysing the biological material for the presence of a target nucleic acid sequence. | 09-11-2008 |
| 20080220415 | Detection Method of Dna Amplification Using Probe Labeled With Intercalating Dye - The present invention relates to a detection method of nucleic acid amplification using probe labeled with intercalating dye. More particularly, the present invention is directed to a real-time detection method of nucleic acid amplification, comprising the steps of i) producing an aqueous buffer which contains a nucleic acid, a pair of primers for amplification of said nucleic acid, a fluorescent probe wherein a fluorescent dye of which intensity of fluorescence is varied when the dye is intercalated into a double-stranded nucleic acid, is connected with an oligonucleotide of which base sequence is complementary with at least a part of said nucleic acid, four (4) kinds of nucleotides and DNA polymerase; ii) denaturing said doublestranded nucleic acid into single strands by heating the aqueous buffer prepared in step i) up to 931 C to 96 C; iii) annealing said pair of primers with said single strand by cooling the solution obtained in step ii) up to 50 C. to 571 C; iv) replicating said single-stranded nucleic acid by heating the solution obtained from step iii) up to 701 C to 74° C.; v) denaturing said replicated nucleic add into single strands by heating the solution obtained in step iv) up to 931 C to 961 C; vi) annealing said fluorescent probe with said single-stranded nucleic acid by cooling the solution obtained in step v up to 501 C to 57 C; vii) measuring an intensity of the fluorescence emitted from the solution obtained in step vi); and viii) repeating more than one steps iv) through vii). | 09-11-2008 |
| 20080220417 | Novel Alanine Transaminase Enzymes and Methods of Use - Novel alanine transaminase (ALT) polypeptides and the use thereof as a diagnostic marker to predict and monitor tissue damage and/or tissue malfunction. The ALT polypeptides are murine and/or | 09-11-2008 |
| 20080220419 | Method of Isolating P450 Gene - The present invention provides a method for preparing a hybrid gene. The method includes a step of amplifying a P450 gene fragment contained in a sample using primers designed on the basis of regions of a plurality of P450 in which amino acid sequences are highly conserved and a step of preparing the hybrid gene using the amplified fragments and a known P450 gene. The method includes no culturing step or a step of normalizing extracted DNAs and is useful in isolating a P450 gene from various microbial resources. | 09-11-2008 |
| 20080220420 | Method of Detecting Gene Polymorphism, Method of Diagnosing, Apparatus Therefor, and Test Reagent Kit - The object of the invention is to carry out typing for multiple SNP sites automatically from the stage of sample preparation. A mixture of sample ( | 09-11-2008 |
| 20080220421 | ISOTHERMAL SCREENING FOR NUCLEIC ACIDS ASSOCIATED WITH DISEASES AND CONDITIONS OF THE GI TRACT - The presently described technology relates generally to the art of molecular diagnostics and more particularly to point-of-care diagnostic methods and materials. The diagnostic methods and materials of the presently described technology are suitable for a variety of uses including but not limited to the bedside or field diagnosis of infectious or noninfectious diseases. | 09-11-2008 |
| 20080220422 | RARE CELL ANALYSIS USING SAMPLE SPLITTING AND DNA TAGS - The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample. | 09-11-2008 |
| 20080220423 | Oligonucleotide probes useful for detection and analysis of microRNA precursors - The invention relates to ribonucleic acids and oligonucleotide probes useful for detection and analysis of microRNA precursors and their targets. The invention furthermore relates to oligonucleotide probes for detection and analysis of other non-coding RNAs, mRNAs, mRNA splice variants, allelic variants of single transcripts, mutations, deletions, or duplications of particular exons in transcripts, e.g., alterations associated with human disease, such as cancer. | 09-11-2008 |
| 20080220424 | Method for Predicting Responsiveness to Drugs - The present invention provides a novel method to determine the likelihood of effectiveness of a treatment in an individual affected with or at risk for developing cancer. The method involves detecting the presence or absence of Met amplification in an individual. The presence of Met amplification indicates that a Met targeting treatment is likely to be effective. Preferably, the Met targeting treatment is PHA-665752 or PF-02341066. In addition, the present methods allow for the detection of cancer in an individual, wherein the presence of Met amplification indicates that cancer is present and further that it will be treatable, namely with a Met targeting treatment. | 09-11-2008 |
| 20080220425 | Methods and Compositions for Detecting Target Sequences - The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for amplifying a synthetic DNA from a target nucleic acid, forming a nucleic acid cleavage structure on the synthetic DNA, and detecting cleavage of the nucleic acid cleavage structure as an indicator of the preset of the target nucleic acid. | 09-11-2008 |
| 20080220426 | MN Gene and Protein - Identified herein is the location of the MN protein binding site, and MN proteins/polypeptides that compete for attachment to vertebrate cells with immobilized MN protein. Such MN proteins/polypeptides prevent cell-cell adhesion and the formation of intercellular contacts. The MN protein binding site is a therapeutic target that can be blocked by organic or inorganic molecules, preferably organic molecules, more preferably proteins/polypeptides that specifically bind to that site. Therapeutic methods for inhibiting the growth of preneoplastic/neoplastic vertebrate cells that abnormally express MN protein are disclosed. Vectors are provided that encode the variable domains of MN-specific antibodies and a flexible linker polypeptide separating those domains. Further vectors are disclosed that encode a cytotoxic protein/polypeptide operatively linked to the MN gene promoter or a MN gene promoter fragment comprising the HIF-1 consensus binding sequence, and which vectors preferably further encode a cytokine. The MN gene promoter is characterized, and the binding site for a repressor of MN transcription is disclosed. Further, the hypoxia inducibility of the MN gene and the uses of such inducibility are disclosed. | 09-11-2008 |
| 20080220427 | TEST FOR DETERMINING BLOOD TYPE IN THE CAT - The present invention provides compositions and methods for detecting mutations associated with blood type determination in the cat. | 09-11-2008 |
| 20080220428 | COMPOSITIONS AND METHODS FOR DETECTING METHICILLIN-RESISTANT S. AUREUS - The present invention relates to methods of detecting the presence or absence of methicillin-resistant | 09-11-2008 |
| 20080220429 | Single nucleotide polymorphisms and the identification of lactose intolerance - The present invention relates generally to methods, kits, genotyping and/or nucleic acid molecules associated with the identification of a predisposition for lactase persistence, lactase non-persistence, lactose tolerance and/or lactose intolerance. The methods of the present invention comprise in general determining the presence or absence of at least one variant allele having one or more single nucleotide polymorphisms within a gene associated with the expression of lactase-phlorizin hydrolase. The single nucleotide polymorphism is selected from the group consisting essentially of C-14010, G-13915 and G-13907, as measured from the start of the LCT gene. | 09-11-2008 |
| 20080220430 | Methods for assessing risk for cardiac dysrythmia in a human subject - The present invention relates to methods for assessing the risk of a patient for developing a potentially fatal cardiac dysrhythmia and for diagnosing Andersen's Syndrome. A tissue sample from a patient is obtained and the DNA or proteins of the sample isolated. From the DNA and protein isolates the sequence of the KCNJ2 gene or the Kir2.1 polypeptide can be obtained. The KCNJ2 gene or the Kir2.1 can be screened for alteration as compared to the wile-type sequence. An alteration in a copy of the KCNJ2 gene or a Kir2.1 polypeptide indicates that the patient has a high risk for developing a cardiac dysrhythmia and can be diagnosed with Andersen's Syndrome. The invention also related to isolated nucleic acid molecules with one or more alterations as compared to the wild-type sequence. | 09-11-2008 |
| 20080220431 | Cell fusion method - The invention provides methods for fusing a first cell with a second cell to form a hybrid cell. The methods involve incubating a first parental cell producing a first partner of a fusogenic binding partner pair on its surface with a second parental cell producing a second partner of the fusogenic binding partner pair on its surface. In certain embodiments, the parental cells are incubated with a known fusogen such as polyethylene glycol and the fusogenic binding partner pair increases the rate of cell fusion. In many embodiments, the first cell is an antibody producing cell, the second cell is an immortal cell, and the hybrid cell is a hybridoma cell that produces a monoclonal antibody. Also provided by the invention are methods for producing hybridoma cells, and methods for screening those cells for production of a monoclonal antibody of interest. The invention further provides systems and kits for carrying out the subject methods. The subject methods, systems, and kits find use in a variety of different industrial, medical and research applications. | 09-11-2008 |
| 20080220432 | Optimized host cells for protein production - The present invention relates to methods for isolating cells that express increased levels of an RNA or protein of interest, wherein the cells exhibit altered growth profiles, such as cells with increased or decreased rates of proliferation, increased or decreased rates of apoptosis, or cells with a biphasic growth profile. | 09-11-2008 |
| 20080220433 | DETECTING METHYLATED MAMMALIAN NUCLEIC ACID IN STOOL - This document includes methods and materials for enriching and detecting cancer markers. For example, this document includes methods and materials for enriching methylated mammalian nucleic acid from stool samples. | 09-11-2008 |
| 20080220434 | Detection Of Molecule Proximity - The present invention provides methods, compositions and kits for identifying molecules such as proteins or nucleic acids that are found in proximity to each other in vitro or in vivo. For example, the present invention provides for the modification of one or more molecules that are complexed with, or in proximity to, a target biomolecule, wherein the modification of the one or more complexed or proximal molecules is detected. | 09-11-2008 |
| 20080220435 | METHOD FOR DETECTING TARGET SUBSTANCE UTILIZING PROBE DESORPTION - It is an object of the present invention to provide a method for detecting a target substance in a specimen with the use of fine particles, whereby the target substance can be readily detected with the exclusive use of a single type of probe and the detection limit is improved. The present invention provides a method for detecting a target substance in a specimen which comprises the steps of: allowing a complex of a fine particle and a probe to come into contact with a specimen; and detecting changes in physical properties of the fine particle that are caused by desorption of the probe from the fine particle due to interaction between the target substance in the specimen and the probe. | 09-11-2008 |
| 20080220436 | METHODS AND COMPOSITIONS FOR RAPID LIGHT-ACTIVATED ISOLATION AND DETECTION OF ANALYTES - The present invention relates to novel methods for isolating a target molecule from a sample suspected of containing the target molecule. The methods of the present invention utilize solid substrates as a means for capturing, separating, and releasing target molecules, such as chemical or biological compounds. The present invention is specifically directed to novel approaches for capturing, separating and releasing such target molecules. | 09-11-2008 |
| 20080220437 | Substrate Material for Handling and Analysing Samples - The invention relates to a substrate material for analyzing one or more fluid samples for the presence, amount or identity of one or more analytes in the samples, whereby the substrate material is adapted in that way that a flow of the sample or parts thereof in and/or with the substrate material is influenced and/or caused by phase transitions, preferably temperature-inducible phase transitions, in selected areas of the substrate material. | 09-11-2008 |
| 20080220438 | Telomerase Promoter Sequences for Screening Telomerase Modulators - Telomerase reverse transcriptase is part of the telomerase complex responsible for maintaining telomere length and increasing the replicative capacity of progenitor cells. Telomerase activity is turned off in mature differentiated cells, but is turned back on again in hyperplastic diseases, including many cancers. This disclosure provides regulatory elements that promote transcription in cells that express telomerase reverse transcriptase (TERT). The disclosure also provides systems using TERT promoter sequences for identifying compounds that can be used to modulate telomerase expression | 09-11-2008 |
| 20080220439 | Microorganism detecting kit, microorganism counting apparatus, and microorganism counting process - A microorganism detecting kit according to the present invention includes a self-adhesive layer | 09-11-2008 |
| 20080227085 | Methods and Systems for the Identification of Rna Regulatory Sequences and Compounds that Modulate their Function - The present invention is directed to a non-cell based method for identifying RNA regulatory sequences involved in translational control. The method includes: combining a translational extract; an RNA test sequence; and a reporter mRNA under suitable conditions for translation of the reporter mRNA. The method also includes measuring the effect of the test sequence on the translation of the reporter mRNA, wherein a test sequence that modifies the translation of the reporter mRNA includes an RNA regulatory element. The invention also provides methods and systems for identifying test compounds that modulate the regulatory activity of an RNA regulatory sequence. In the method, an RNA regulatory sequence, which regulates translation of a reporter mRNA, is combined with a translation extract; the reporter mRNA; and at least one test compound. The method further includes measuring the effect of the test compound on the ability of the RNA regulatory sequence to regulate the translation of the reporter mRNA. | 09-18-2008 |
| 20080227086 | Test System and Method for the Detection of Analytes - The invention relates to analytical test systems and analytical methods, in which molecular switches are used for the qualitative and quantitative determination of analytes in a sample, which have a broad application by means of a selection of the molecular switch suitable for the analyte. The molecular switch comprises a probe, preferably a nucleic acid or a nucleic acid derivative, coupled to a catalytic component, preferably an enzyme. The analyte induces a conformation change in the probe, which alters the accessibility for a substrate in the probe to catalytic components and the change in substrate turnover, corresponding to the change in catalytic activity, is measured. | 09-18-2008 |
| 20080227087 | Sequences for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) of MREJ types xi to xx - Described herein are novel SCCmec right extremity junction (MREJ) sequences for the detection and/or identification of methicillin-resistant | 09-18-2008 |
| 20080227088 | Approaches to Identify Less Harmful Tobacco and Tobacco Products - Aspects of the invention concern methods for detecting, identifying and evaluating tobacco and tobacco products to determine the potential that these compositions have to contribute to a tobacco-related disease. It is based, at least in part; on the discovery that exposure of pulmonary cells to smoke or smoke condensate obtained from tobacco or tobacco products induces double stranded breaks in cellular DNA, which were efficiently detected using assays that measure the presence, absence, or amount of phosphorylation of the histone, H2AX. | 09-18-2008 |
| 20080227089 | Method of Diagnosing and/or Predicting the Development of an Allergic Disorder - The present invention relates to methods for diagnosing an allergic disorder, predicting the development of an allergic disorder in an animal, monitoring the progress of therapy targeted at an allergic disorder, classification of the allergic disorder into one or more clinical/immunological phenotypes, and/or determining the potentional responsiveness of individual animals suffering from or at risk of an allergic disorder to particular forms of therapy. In particular, the present invention relates to a method of diagnosing and/or predicting the development of an allergic disorder in an animal, comprising the step of analysing a biological sample from the animal to determine the level of activation of one or more allergy-associated genes, in which the level of activation is diagnostic of the allergic disorder or predicative of the relative risk for the development of an allergic disorder in the animal. | 09-18-2008 |
| 20080227090 | METHODS, APPARATUS, AND COMPUTER PROGRAMS FOR VERIFYING THE INTEGRITY OF A PROBE - The present invention provides methods, apparatuses and computer programs for verifying the integrity of a probe by comparing the fluorescence value of a probe to a threshold value. The invention also provides for methods, apparatuses and computer programs for normalizing the fluorescence value of a probe and detecting a target nucleic acid in a sample. | 09-18-2008 |
| 20080227091 | QTL controlling Sclerotinia stem ROT resistance in soybean - Markers associated with | 09-18-2008 |
| 20080227092 | Solid-Phase Oligosaccharide Tagging: a Technique for Manipulation of Immobilized Carbohydrates - The invention relates to methods of manipulating immobilised carbohydrates by derivatisation. Depending on the nature of the derivatisation, the carbohydrate may thereby be more easily detected and/or identified or handled. In particular, the invention relates to methods of preparing a reactive sugar comprising the steps of: i) providing a sample comprising a reducing sugar; ii) providing a solid support covalently attached to a linker comprising a capture group comprising an —NH2 group, wherein said linker optionally is attached to said solid support via a spacer; iii) reacting said reducing sugar with said —NH2 group, thereby obtaining an immobilised sugar; iv) reacting free —NH2 groups with a capping agent, wherein the capping agent comprises a reactive group capable of reacting with an —NH2 group; and v) reducing C═N bonds with a reducing agent, thereby obtaining an reactive sugar of the structure SugarCH | 09-18-2008 |
| 20080227093 | PHENYLTHIOCARBAMIDE (PTC) TASTE RECEPTOR - The invention provides isolated nucleic and amino acid sequences of a taste cell receptor that serves as a sensor for the bitter taste of phenylthiocarbamide (PTC), antibodies to such PTC taste receptor, methods of detecting such nucleic and amino acid sequences, and methods of screening for modulators of such PTC taste receptor. | 09-18-2008 |
| 20080227094 | METHOD OF JUDGING LYMPH NODE METASTASIS OF STOMACH CANCER - Methods of judging the lymph node metastasis of stomach cancer, apparatuses for judging the lymph node metastasis of stomach cancer and kits used therefor are disclosed. | 09-18-2008 |
| 20080227095 | Systems and methods of analyzing nucleic acid polymers and related components - Systems and methods of identifying, sequencing and/or detecting nucleic acid polymers, as well as related components (e.g., substrates, software and the like) are disclosed. | 09-18-2008 |
| 20080227096 | Assay for response to proteasome inhibitors - The invention relates to a method for predicting a response to a proteasome inhibitor in the prophylaxis or treatment of a cancer in an individual. The method comprises providing a sample of cancer cells of the cancer from the individual, and evaluating the level of at least one molecule in the cancer cells associated with the unfolded protein response of the cancer cells, to provide test data indicative of the level of activity of the unfolded protein response. The test data is used to predict the response of the cancer cells to the proteasome inhibitor. The evaluation of the level of the molecule can be utilized for determination of treatment for the cancer. | 09-18-2008 |
| 20080227097 | FLUORESCENT PROTEIN AND CHROMOPROTEIN - It is an object of the present invention to provide a novel chromoprotein and a novel fluorescent protein. The present invention provides chromoproteins derived from | 09-18-2008 |
| 20080227098 | METHOD FOR DIAGNOSING CANCER OF THE PROSTATE - Methods for diagnosis and prognosis of prostate cancer are provided. The methods involve the detection of the level of expression of Claudin-1 in tissue or cell samples. Claudin-1 is underexpressed or non-expressed in the majority of prostate cancers. | 09-18-2008 |
| 20080227099 | COMPOSITIONS AND METHODS FOR INCREASING CHOLESTEROL EFFLUX AND RAISING HDL USING ATP BINDING CASSETTE TRANSPORTER PROTEIN ABC1 - The present invention relates to novel ABC1 polypeptides and nucleic acid molecules encoding the same. The invention also relates to recombinant vectors, host cells, and compositions comprising ABC1 polynucleotides, as well as to methods for producing ABC1 polypeptides. The invention also relates to antibodies that bind specifically to ABC1 polypeptides. In addition, the invention relates to methods for increasing cholesterol efflux as well as to methods for increasing ABC1 expression and activity. The present invention further relates to methods for identifying compounds that modulate the expression of ABC1 and methods for detecting the comparative level of ABC1 polypeptides and polynucleotides in a mammalian subject. The present invention also provides kits and compositions suitable for screening compounds to determine the ABC1 expression modulating activity of the compound, as well as kits and compositions suitable to determine whether a compound modulates ABC1-dependent cholesterol efflux. | 09-18-2008 |
| 20080227100 | METHOD FOR ESTIMATING hERG INHIBITION OF DRUG CANDIDATES USING MULTIVARIATE PROPERTY AND PHARMACOPHORE SAR - The present invention provides a computational model and methods of use thereof for predicting whether a compound is likely to inhibit K | 09-18-2008 |
| 20080227101 | METHODS FOR TESTING MILK - The disclosure is related generally to methods for testing mammary fluid (including milk) to establish or confirm the identity of the donor of the mammary fluid. Such methods are useful in the milk-bank business to improve safety. | 09-18-2008 |
| 20080227102 | Methods for Identification of Merle Gene - Animals with mutations in SILV present with the merle coat color pattern phenotype. Methods for the identification of animals that harbor a mutation in the SILV gene are described. Mutations in the SILV gene can be identified from any biological sample such as a cell or tissue that contains genomic DNA. A microsatellite marker identified using linkage disequilibrium mapping that segregates with merle is also described. | 09-18-2008 |
| 20080227103 | Method for identification of polynucleotides capable of cleaving target mRNA sequences - Provided are methods for identifying a polynucleotide that can reduce the level of a target mRNA. The method can be performed by providing cells that express an RNA polynucleotide that contains a target mRNA sequence, an internal ribosome entry sequence (IRES) and a sequence encoding a secreted reporter protein; introducing to the cells a test polynucleotide, and measuring activity of the secreted reporter protein. A reduction in secreted reporter protein activity relative to a control cell into which the test polynucleotide has not been introduced is indicative that the test polynucleotide is capable of reducing the level of the target mRNA in the cells. The method is adaptable for high throughput screening methods and is suited for identifying polynucleotides that can catalyze cleavage of target mRNA and/or act on target mRNA through and antisense or RNAi mechanism. | 09-18-2008 |
| 20080227104 | Primer, primer set, and nucleic acid amplification method and mutation detection method using the same - The present invention provides a primer that effectively can detect, for example, the double helix structure of a nucleic acid. The primer is a labeled nucleic acid containing at least one structure represented by the following formula (16), | 09-18-2008 |
| 20080227105 | Method for diagnosing diseases - An improved method for diagnosing autoimmune or genetic diseases is provided in this disclosure. In particular, a method to diagnose diseases affecting the tissues of organs selected from the group of spleen, brain, heart, kidney, thyroid, eye, skin, intestine, liver, pancreas, adrenal gland, prostate and lungs or from the tissues selected from the group of muscles and bones or other tissues. More specifically, the present invention provides an improved method for diagnosing diseases affecting neuromuscular junctions. | 09-18-2008 |
| 20080227106 | Compositions and methods for the diagnosis and treatment of tumor - The present invention is directed to compositions of matter useful for the diagnosis and treatment of tumor in mammals and to methods of using those compositions of matter for the same. | 09-18-2008 |
| 20080227107 | COMPOSITIONS AND METHODS FOR LABELING OF NUCLEIC ACID MOLECULES - The present invention is generally related to compositions, kits and methods for labeling nucleic acid molecules using reverse transcriptases, preferably multi-subunit reverse transcriptases such as ASLV reverse transcriptases. Specifically, the invention relates to methods, kits and compositions for fluorescently labeling nucleic acid molecules during nucleic acid synthesis. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection and diagnostics. | 09-18-2008 |
| 20080227108 | MOLECULAR IDENTIFICATION OF ASPERGILLUS SPECIES - Novel techniques for the detection of | 09-18-2008 |
| 20080227109 | Risk assessment for adverse drug reactions - The present invention provides a method of predicting the risk of a patient for developing adverse drug reactions, particularly SJS or TEN. It was discovered that an HLA-B allele, HLA-B* 1502, is associated with SJS/TEN that is induced by a variety of drugs. The correlation with HLA-B* 1502 is most significant for carbamazepine-induced SJS/TEN, wherein all the patients tested have the HLA-B* 1502 allele. In addition, another HLA-B allele, HLA-B*5801, is particularly associated with SJS/TEN induced by allopurinol. Milder cutaneous reactions, such as maculopapular rash, erythema multiforme (EM), urticaria, and fixed drug eruption, are particularly associated with a third allele, HLA-B *4601. For any of the alleles, genetic markers (e.g., HLA markers, microsatellite, or single nucleotide polymorphism markers) located between DRB 1 and HLA-A region of the specific HLA-B haplotype can also be used for the test. | 09-18-2008 |
| 20080233563 | Enhanced Detection of Rna Using a Panel of Truncated Gene-Specific Primers for Reverse Transcription - The present invention provides truncated gene-specific primers in panels that can be used during the reverse transcription step of RT-PCR to increase signal detection of cancer gene markers in a tissue sample. Also provided are forward and reverse primers for RT-PCR. Methods of using the primers are also provided. | 09-25-2008 |
| 20080233565 | PKHDL1, a homolog of the autosomal recessive kidney disease gene - Nucleic acids encoding fibrocystin-L polypeptides and fibrocystin-L polypeptides are provided. Antibodies against the polypeptides, vectors and host cells containing the nucleic acids, methods for using the nucleic acids and polypeptides, and compositions and articles of manufacture also are provided. | 09-25-2008 |
| 20080233566 | Methods and materials for detecting mutations in quasispecies having length polymorphisms - The present invention is directed to a method for detecting the presence or absence of a mutation of interest in the nucleic acid of a pathogen, wherein the mutation of interest is located adjacent to a length polymorphism defining multiple quasispecies of the pathogen. | 09-25-2008 |
| 20080233567 | Companion diagnostic assays for cancer therapy - A method for classifying cancer patients as eligible to receive cancer therapy with a small molecule inhibitor of Bcl-2 comprising determination of the presence or absence in a patient tissue sample of chromosomal copy number status at the chromosomal locus 13q14 comprising the microRNA's miR15 | 09-25-2008 |
| 20080233568 | Detection of Extracellular Tumor-Associated Nucleic Acid in Blood Plasma or Serum Using Nucleic Acid Amplification Assays - This invention relates to detection of specific extracellular nucleic acid in plasma or serum fractions of human or animal blood associated with neoplastic or proliferative disease. Specifically, the invention relates to detection of nucleic acid derived from mutant oncogenes or other tumor-associated DNA, and to those methods of detecting and monitoring extracellular mutant oncogenes or tumor-associated DNA found in the plasma or serum fraction of blood by using rapid DNA extraction followed by nucleic acid amplification with or without enrichment for mutant DNA. In particular, the invention relates to the detection, identification, or monitoring of the existence, progression or clinical status of benign, premalignant, or malignant neoplasms in humans or other animals that contain a mutation that is associated with the neoplasm through detection of the mutated nucleic acid of the neoplasm in plasma or serum fractions. The invention permits the detection of extracellular, tumor-associated nucleic acid in the serum or plasma of humans or other animals recognized as having a neoplastic or proliferative disease or in individuals without any prior history or diagnosis of neoplastic or proliferative disease. The invention provides the ability to detect extracellular nucleic acid derived from genetic sequences known to be associated with neoplasia, such as oncogenes, as well as genetic sequences previously unrecognized as being associated with neoplastic or proliferative disease. The invention thereby provides methods for early identification of colorectal, pancreatic, lung, breast, bladder, ovarian, lymphoma and all other malignancies carrying tumor-related mutations of DNA and methods for monitoring cancer and other neoplastic disorders in humans and other animals. | 09-25-2008 |
| 20080233569 | Detection of Extracellular Tumor-Associated Nucleic Acid in Blood Plasma or Serum Using Nucleic Acid Amplification Assays - This invention relates to detection of specific extracellular nucleic acid in plasma or serum fractions of human or animal blood associated with neoplastic or proliferative disease. Specifically, the invention relates to detection of nucleic acid derived from mutant oncogenes or other tumor-associated DNA, and to those methods of detecting and monitoring extracellular mutant oncogenes or tumor-associated DNA found in the plasma or serum fraction of blood by using rapid DNA extraction followed by nucleic acid amplification with or without enrichment for mutant DNA. In particular, the invention relates to the detection, identification, or monitoring of the existence, progression or clinical status of benign, premalignant, or malignant neoplasms in humans or other animals that contain a mutation that is associated with the neoplasm through detection of the mutated nucleic acid of the neoplasm in plasma or serum fractions. The invention permits the detection of extracellular, tumor-associated nucleic acid in the serum or plasma of humans or other animals recognized as having a neoplastic or proliferative disease or in individuals without any prior history or diagnosis of neoplastic or proliferative disease. The invention provides the ability to detect extracellular nucleic acid derived from genetic sequences known to be associated with neoplasia, such as oncogenes, as well as genetic sequences previously unrecognized as being associated with neoplastic or proliferative disease. The invention thereby provides methods for early identification of colorectal, pancreatic, lung, breast, bladder, ovarian, lymphoma and all other malignancies carrying tumor-related mutations of DNA and methods for monitoring cancer and other neoplastic disorders in humans and other animals. | 09-25-2008 |
| 20080233570 | METHODS FOR IDENTIFICATION OF SEPSIS-CAUSING BACTERIA - The present invention provides compositions, kits and methods for rapid identification and quantification of sepsis-causing bacteria by molecular mass and base composition analysis. | 09-25-2008 |
| 20080233571 | Method for identifying compounds which affect synaptogenesis - A method is provided for identifying a compound which affects the formation of AMPA receptors into aggregates. A method is also provided for identifying a compound which affects the formation of synaptic connections. A method is provided for identifying a compound that modulates immediate early gene expression. A method is further provided for increasing the number of excretory synapses of a neuron, including introducing into the neuron a polynucleotide sequence encoding a Narp operatively linked to a promoter, or a Narp polypeptide, thereby increasing the number of excretory synapses of the neuron. A method is provided for treating a subject with a disorder associated with a decrease in a function or expression of Narp, including administering to the subject a therapeutically effective amount of a compound that augments Narp function or expression. A method is provided for treating a subject with a disorder associated with an increase in a function or expression of Narp, including administering to the subject a therapeutically effective of a compound that inhibits Narp function or expression. A method is provided for treating a patient having or at risk of having a disorder associated with decreased Narp expression. The method includes introducing into a cell of a patient having a disorder associated with decreased Narp expression or function a polynucleotide sequence encoding a Narp polypeptide operatively linked to a promoter. A method is provided for treating a subject having a deficiency in a neuron's immediate early gene responsiveness to a stimulus. The method includes administering a nucleic acid encoding a Narp polypeptide to said subject, wherein the administration results in amelioration of the deficiency. | 09-25-2008 |
| 20080233572 | METHODS AND COMPOSITIONS FOR THE DETECTION AND QUANTIFICATION OF E.COLI AND ENTEROCOCCUS - The present invention is drawn to methods and compositions for the rapid assessment of fecal indicator bacteria in a sample. Provided herein are novel primer and probe compositions for use in detecting the presence of these organisms in a sample, particularly using quantitative PCR methods. Provided herein are novel oligonucleotide primers and probes, including the primers set forth in SEQ ID NO:1-4, the novel oligonucleotide probe sequences set forth in SEQ ID NO:5-8, and methods for using these primers and probes for the detection and/or quantification of fecal indicator bacteria, particularly | 09-25-2008 |
| 20080233573 | Gene expression profiling for identification, monitoring and treatment of transplant rejection - The present invention provides methods of characterizing organ transplant rejection or inflammatory conditions associated with organ transplant rejection using gene expression profiling. | 09-25-2008 |
| 20080233574 | Map-Based Genome Mining Method for Identifying Regulatory Loci Controlling the Level of Gene Transcripts and Products - The invention pertains to a method for identifying one or more regions within a genome of an organism of interest that mediate the expression of one or more genes of interest. The method comprises identifying a first and a second organism of interest, the first organism of interest is characterized by exhibiting a measurable response to an environmental stimulus, or otherwise, exhibiting a phenotype associated with differential gene expression associated with a process of interest. The second organism of interest is characterized by lacking or not exhibit as strong a response to the stimulus as that observed within the first organism of interest, or it exhibits a different phenotype compared with that of the first organism of interest, wherein the different phenotype is associated with the process of interest, or it exhibits a phenotype of interest that segregates when compared with the first organism of interest, or a combination thereof. The first and second organisms of interest are crossed to produce a population of segregated progeny and RNA is extracted from each of the segregated progeny. The level of gene expression for one or more genes of interest that are associated with the response to an environmental stimulus, or process of interest is quantified. A linkage map of the segregated progeny using one or more markers is prepared, and the relationship between said one or more markers on the linkage map and the gene expression of the one or more genes of interest is determined and one or more quantitative trait loci (QTL) identified. This method also pertains to identifying one or more QTLs associated with one or more genes of interest in segregated progeny that are subjected a desired environmental stimulus. Furthermore, this method may be used for the identification of one or more QTL corresponding to a transcription factor or any factor controlling the expression of one or more genes of interest, and the one or more genes located at the one or more QTL may be isolated and characterized. | 09-25-2008 |
| 20080233575 | Methods for increasing accuracy of nucleic scid sequencing - The invention provides methods for improving the fidelity of a sequencing-by-synthesis reaction by resequencing at least a portion of a nucleic acid template. | 09-25-2008 |
| 20080233576 | METHOD FOR FEATURE SELECTION IN A SUPPORT VECTOR MACHINE USING FEATURE RANKING - In a pre-processing step prior to training a learning machine, pre-processing includes reducing the quantity of features to be processed using feature selection methods selected from the group consisting of recursive feature elimination (RFE), minimizing the number of non-zero parameters of the system (l | 09-25-2008 |
| 20080233577 | METHOD FOR PRODUCING LIGANDS, LIGANDS AND TEST KIT - The invention relates to a method for producing ligands, in particular aptamers. With this method, a target substance is offered to a set of candidate ligands, and the unbonded ligands are separated out by a cross-flow filtration process. The retentate, which contains ligand-target substance complexes, then undergoes further continuous cross-flow filtration while chemical and/or physical parameters are being varied. After each variation of a parameter, those candidate ligands whose bond with the target substance was dissolved by the parameter variation are collected in separate fractions and separately multiplied. | 09-25-2008 |
| 20080233578 | METHOD FOR DETECTING MUTATION OF NUCLEIC ACID USING SINGLE-STRANDED DNA-BINDING PROTEIN - A method for judging the presence or absence of a mutation in a nucleic acid sequence, the method includes utilizing a single-stranded DNA-binding protein; the aforementioned method for judging the presence or absence of a mutation in a nucleic acid sequence, wherein the aforementioned presence or absence of a mutation in a nucleic acid sequence is judged by a product formed by a nucleic acid amplification reaction utilizing the single-stranded DNA-binding protein; and a kit for judging the presence or absence of the aforementioned mutation in a nucleic acid sequence. | 09-25-2008 |
| 20080233579 | PRIMER SET FOR DETECTING OVEREXPRESSION OF KATP CHANNEL AND KIT COMPRISING SAID PRIMER SET - The present invention relates to a primer set for confirming an increase of mRNA in an ATP-sensitive potassium channel (K | 09-25-2008 |
| 20080233580 | Transgenic animal model - The present invention is related to a transgenic, non-human animal, particularly a transgenic rodent, but especially a transgenic mouse model which allows for the simultaneous, tissue-specific and temporally-controlled regulation of transgene expression and can be used as a tool to investigate the consecutive steps involved in initiation and progression of certain diseases such as cancer, but particularly lung cancer. | 09-25-2008 |
| 20080233581 | HISTONE DEACETYLASE AND METHODS OF USE THEREOF - The present invention provides nucleic acid molecules that encode histone deacetylase, as well as recombinant vectors and host cells that include the subject nucleic acid molecules. Also provided are histone deacetylase polypeptide compositions. The histone deacteylase nucleic acid molecules are useful in a variety of diagnostic and therapeutic applications, which are also provided. | 09-25-2008 |
| 20080233582 | SINGLE NUCLEOTIDE POLYMORPHISMS ASSOCIATED WITH SUSCEPTIBILITY TO CARDIOVASCULAR DISEASE - The present invention provides SNPs, polymorphic variants, and haplotypes associated with cardiovascular disease. The invention also provides methods for detecting the SNPs, polymorphic variants, and haplotypes. The invention also provides methods for determining an individual's genotype with respect to one or more polymorphisms and/or haplotypes associated with cardiovascular disease. The invention further provides methods of determining whether an individual has or is susceptible to development or occurrence of a cardiovascular disease or event. The methods are useful for providing diagnostic and/or prognostic information, selecting therapeutic regimens, etc. The invention further provides reagents and kits for practicing the methods. | 09-25-2008 |
| 20080233583 | BIOMARKERS FOR PREECLAMPSIA - The present invention provides methods for predicting the development of and diagnosing preeclampsia, providing a prognosis, and predicting recurrence of the disease using molecular markers that are overexpressed or underexpressed in preeclampia. Also provided are methods to identify compounds that are useful for the treatment or prevention of preeclampsia. | 09-25-2008 |
| 20080233584 | RNAi MODULATION OF MLL-AF4 AND USES THEREOF - The invention relates to compositions and methods for modulating the expression of the MLL-AF4 fusion gene, and more particularly to the downregulation of MLL-AF4 by chemically modified oligonucleotides. | 09-25-2008 |
| 20080233585 | USE OF DIFFERENTIALLY EXPRESSED NUCLEIC ACID SEQUENCES AS BIOMARKERS FOR CANCER - The present invention relates to novel marker sequences that are differentially expressed in cancer cells or tissue of a subject with cancerous conditions. The present invention also relates to assays for diagnosis, prognosis, staging, monitoring, therapeutic treatment, and marker sequence related agents including probes, primers, antibodies, and therapeutic compositions. | 09-25-2008 |
| 20080233586 | METHODS FOR PREPARING AND PREFORMING ANALYSIS - The invention relates to methods for preparing and performing quantitative PCR analyses, a new sealing device and a new use. According to the invention, a sample vessel containing the samples to be analyzed is sealed by placing a planar sealing device on the vessel to cover the samples and applying pressure on the sealing device in order to deform the sealing device so as to form a light-refracting geometry individually for the samples to be analyzed. The invention offers a convenient way of sealing the vessel and forming analysis-improving optical lenses over the samples simultaneously. | 09-25-2008 |
| 20080233587 | METHOD FOR DIRECT AMPLIFICATION FROM CRUDE NUCLEIC ACID SAMPLES - The present teachings relate to improved methods, kits, and reaction mixtures for amplifying nucleic acids. In some embodiments a novel direct buffer formulation is provided which allows for the direct amplification of the nucleic acids in a crude sample with minimal sample purification. | 09-25-2008 |
| 20080233588 | Analytical Method and Kit - Analytical methods using RNA-containing probes for the detection or analysis of nucleic acid sequences is described. These probes are contacted with a sample suspected of containing the nucleic acid sequence and if they form duplexes, they are hydrolysed. This may be done, for example during an amplification reaction. AMP generated as a result of the hydrolysis is converted to ATP. The ATP may then be detected using bioluminescent reagents. Inclusion of modified adenosine in at least one probe means that the signal arising from one probe will give rise to a different and distinguishable bioluminescent signal thus enabling the use of for example an internal control in bioluminescently-reported nucleic acid tests. | 09-25-2008 |
| 20080233589 | Complementation Systems Utilizing Complexes of Heteroproteins - The present invention provides heterologous complementation systems and methods of using the systems to detect molecular interactions. In particular, the heterologous complementation systems comprise polypeptide fragments derived from heterologous polypeptides. If a molecular interaction occurs, then the heterologous polypeptide fragments are able to associate and produce a detectable signal. | 09-25-2008 |
| 20080233590 | Device For the Analysis of Liquid Samples - The present invention relates to devices for the analysis of liquid samples, comprising a rotational-symmetric rotor ( | 09-25-2008 |
| 20080233591 | METHOD AND TEST KIT FOR QUANTITATIVE DETERMINATION OF POLYNUCLEOTIDES IN A MIXTURE - The invention relates to a method and test kit for quantitative determination of the amounts or relative proportions of polynucleotides in a mixture. The invention enables assessment of dynamic variations in a mixed population of organisms using affinity aided solution hybridization. The test kit comprises organized pools of polynucleotide probes having approximately the same number of nucleotides, which are distinguishable using resolution enabling tags providing the probes with different sizes. The resolution enabling tags may simultaneously act as tracer, affinity or primer tags. The probes are allowed to hybridize with affinity tagged analyte polynucleotides. The result is hybrids, recoverable on separation aiding tools provided with counterparts of the affinity tag. After the quantitative release of the probes, the individual probes can be amplified and recorded. The method and test kit are useful for determining hygienic and epidemiologic situations and evaluating the effect of antibiotic treatment and sanitary measures. | 09-25-2008 |
| 20080233592 | Assay Method for Group Transfer Reactions - The present invention relates to methods for detecting, quantifying and high throughput screening of donor-products and the catalytic activities generating the donor-products in group-transfer reactions catalyzed by adenosine triphosphatase (ATPase) or guanine triphosphatase (GTPase). The invention further provides immunoassays, antibodies and kits that may be used to practice the methods of the invention. | 09-25-2008 |
| 20080233593 | Pseudo-Tissue for Quality Control and Quality Control Method Using Same - A pseudo-tissue for quality control is described, which includes a nucleic acid component selected from the group consisting of a nucleic acid and a cell including a nucleic acid, and a gel for holding nucleic acid component. | 09-25-2008 |
| 20080241822 | GENOME-WIDE LOCATION AND FUNCTION OF DNA BINDING PROTEINS - The present invention relates to a method of identifying a region (one or more) of a genome of a cell to which a protein of interest binds. In the methods described herein, DNA binding protein of a cell is linked (e.g., covalently crosslinked) to genomic DNA of a cell. The genomic DNA to which the DNA binding protein is linked is removed and combined or contacted with DNA comprising a sequence complementary to genomic DNA of the cell under conditions in which hybridization between the identified genomic DNA and the sequence complementary to genomic DNA occurs. Region(s) of hybridization are region(s) of the genome of the cell to which the protein of binds. A method of identifying a set of genes where cell cycle regulator binding correlates with gene expression and of identifying genomic targets of cell cycle transcription activators in living cells is also encompassed. | 10-02-2008 |
| 20080241823 | Method for Hla Typing - A method for the identification of DNA sequence elements in complex and highly variable sequences is described. The method consists of identifying a short sequence element of several DNA bases (2-6 bases) at a given position in the genome simultaneously on all parental alleles. The method allows differentiating mini-haplotypes on different alleles in one analysis. The method consists of carrying out an enzymatic primer extension reaction with a combination of extension primers (pool of primers) and analysing the products by mass spectrometry. The pool of primers is assembled in such a way that the primer extension product allows unambiguous identification of both the primer of the pool that was extended and the base that was added. The method is of great utility for DNA sequences harbouring many SNPs close to each other with many possible haplotypes. Such sequences are known in the Major Histocompatibility Complex (MHC). This method is particularly well suited for DNA-based HLA typing and in combination with a suitable selection of sites tested, it is superior in ease of operation to conventional HLA typing methods. We have identified sets of these assays for HLA-A, HLA-B, and HLA-DRB 1 that allow unambiguous four-digit HLA of each of these genes with between 11 and 28 queried markers. | 10-02-2008 |
| 20080241824 | Mutation Associated With Lacunar Strokes - The present invention relates to a method of identifying a subject predisposed to lacunar stroke. The method includes the step of identifying in the subject the presence of a thymine to cytosine mutation at position -107 in both alleles of the paraoxonase 1 locus. | 10-02-2008 |
| 20080241825 | Materials and Methods for Treatment of Cancer - Glypican 5 is shown for the first time to have a role in proliferation of cancer cells, including tumours which do not show chromosomal amplification at 13q31. The use of glypican 5 (GPC5) antagonists and binding agents for the treatment of cancer, particularly rhabdomyosarcoma and breast cancer, is disclosed. | 10-02-2008 |
| 20080241826 | Probe And Primer For Tubercle Bacillus Detection, And Method Of Detecting Human Tubercle Bacillus Therewith - An object of the present invention is to provide a novel primer and prove for detecting tubercle | 10-02-2008 |
| 20080241827 | Methods For Detecting A Mutant Nucleic Acid - The invention relates to methods for detection of genomic variation. The invention may be used to analyze nucleic acid sequences to detect low frequency mutations in a sample and/or screen for the presence of a disease. | 10-02-2008 |
| 20080241828 | DETECTION OF DNA METHYLATION USING RAMAN SPECTROSCOPY - Epigenetic events such as DNA methylation play important roles in the regulation of gene expression. DNA methylation patterns have been found to differ between healthy and diseased tissue, such as healthy and cancerous tissue, thereby allowing DNA methylation to serve as a biomarker for disease states. Embodiments of the invention provide methods for detecting methylation patterns in DNA polymers. Methylation patterns are detected, in part, through the use of surface enhanced Raman spectroscopy (SERS). SERS provides a sensitive structure-based technique for chemical analysis. | 10-02-2008 |
| 20080241829 | Methods And Kits For Producing Labeled Target Nucleic Acid For Use In Array Based Hybridization Applications - Methods for producing labeled probe nucleic acids from genomic nucleic acid template are provided. In some embodiments of the subject methods, a plurality of sequence-specific primers are employed to enzymatically generate a set of labeled target nucleic acids corresponding to coding regions of genes from a genomic template via a primer extension protocol. The subject methods find use in a variety of different applications, and can be used, for example, in the preparation of labeled probe nucleic acids for use in array based comparative genomic hybridization applications. Also provided are kits for use in practicing the subject methods. | 10-02-2008 |
| 20080241830 | Digital amplification - The identification of pre-defined mutations expected to be present in a minor fraction of a cell population is important for a variety of basic research and clinical applications. The exponential, analog nature of the polymerase chain reaction is transformed into a linear, digital signal suitable for this purpose. Single molecules can be isolated by dilution and individually amplified; each product is then separately analyzed for the presence of pre-defined mutations. The process provides a reliable and quantitative measure of the proportion of variant sequences within a DNA sample. | 10-02-2008 |
| 20080241831 | Methods for detecting small RNA species - The invention provides a method of detecting small target nucleotide sequences, in particular, small RNA species that are present in a sample. The method generally comprises a poly-A polymerization step or a ligation step to add a universal sequence to the 3′-end of all RNA molecules, followed by a universal primer-mediated cDNA synthesis, solid-phase selection, assay oligo annealing, extension and PCR amplification/labeling. The method of the invention can be practiced to amplify and label a small amount of miRNA or other ncRNA. The resulting amplification product can be read out on a universal array or an array with miRNA-specific or ncRNA-specific probes. The invention has multiple embodiments, including methods, compositions, and kits. In general, the nucleic acids, compositions, and kits comprise materials that are useful in carrying out the methods of the invention or are produced by the methods, and that can be used to detect small target nucleic acid sequences present in samples, in particular, small RNA species. | 10-02-2008 |
| 20080241832 | METHOD OF DETECTING AND QUANTIFYING HEPATITIS C VIRUS - Methods, reagents, and kits for detecting hepatitis C virus (HCV) in biological samples. | 10-02-2008 |
| 20080241833 | SYSTEM AND METHOD FOR NUCLEIC ACID SEQUENCING BY POLYMERASE SYNTHESIS - This invention relates to improved methods for sequencing and genotyping nucleic acid in a single molecule configuration. The method involves single molecule detection of fluorescent labeled PPi moieties released from NTPs as a polymerase extension product is created. | 10-02-2008 |
| 20080241834 | Method for improving neoadjuvant chemotherapy - Disclosed is a method and composition for optimizing the efficiency of breast cancer neoadjuvant chemotherapy, depending on the particular constitutional genotype characteristics of the gene BRCA1 in each patient. Generally, the invention concerns a new method to improve neoadjuvant therapy depending on a particular constitutional genotype. Subject of invention allow to synthesize DNA and identification of germline BRCA1 genetic abnormalities which are correlated with a significantly decreased clinical response to neoadjuvant chemotherapy based on taxane-derived cytostatics in breast cancer patients. | 10-02-2008 |
| 20080241835 | DIFFERENTIALLY EXPRESSED GENES INVOLVED IN ANGIOGENESIS, THE POLYPEPTIDES ENCODED THEREBY, AND METHODS OF USING THE SAME - The present invention is directed to nucleic acid sequences and the polypeptides encoded thereby that are differentially expressed in angiogenesis. Also provided are methods for stimulating or inhibiting angiogenesis in mammals, including humans. Pharmaceutical compositions based on polypeptides, agonists, or antagonists thereto are also provided. Additionally, the invention also provides methods for diagnosing and treating angiogenic disorders including, but not limited to, wound healing and cancer. | 10-02-2008 |
| 20080241836 | PROCESS FOR SELF-ASSEMBLY OF STRUCTURES IN A LIQUID - A process and apparatus for self-assembling a number of elements and determining their sequence is provided. In the field of DNA analysis, an iterative process is disclosed wherein an apparatus with a set of reaction chambers in which a species of recognition element nucleotides are differentially added and subjected to a polymerization reaction allows recognition of which species is next in sequence on a template strand by the effect that synthesis has on a detecting template as measured by a detector in a detection area. Stepwise addition of the identified species then determines if an element repeat exists. The process is repeated until the entire structure is complete and the sequence identified. | 10-02-2008 |
| 20080241837 | Automated Method for Determining the Presence of a Target Nucleic Acid in a Sample - An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations, or modules, in which discrete aspects of the assay are performed on fluid samples contained in reaction receptacles. The analyzer includes stations for automatically preparing a specimen sample, incubating the sample at prescribed temperatures for prescribed periods, performing an analyte isolation procedure, and ascertaining the presence of a target analyte. An automated receptacle transporting system moves the reaction receptacles from one station to the next. The analyzer further includes devices for carrying a plurality of specimen tubes and disposable pipette tips in a machine-accessible manner, a device for agitating containers of target capture reagents comprising suspensions of solid support material and for presenting the containers for machine access thereto, and a device for holding containers of reagents in a temperature controlled environment and presenting the containers for machine access thereto. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte. The process is performed by automatically moving each of a plurality of reaction receptacles containing a solid support material and a fluid sample between stations for incubating the contents of the reaction receptacle and for separating the target analyte bound to the solid support from the fluid sample. An amplification reagent is added to the separated analyte after the analyte separation step and before a final incubation step. | 10-02-2008 |
| 20080241838 | METHODS AND SYSTEMS FOR DETECTING NUCLEIC ACIDS - Methods and kits for detecting a target nucleic acid in a sample are described. In some embodiments, the sample to be analyzed includes a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising nuclease activity that can cleave the hybridized hybridization probe to thereby release a labeled probe fragment. In some embodiments, the sample can then be contacted with a solid support comprising surface bound capture probes which can hybridize to the labeled probe fragment(s). These capture probes more readily bind to the probe fragment(s) than to the intact hybridization probe. The label can then be detected on the support surface. In this manner, improved discrimination between the probe fragments and the intact hybridization probes can be achieved. | 10-02-2008 |
| 20080241839 | Method for correlating differential brain images and genotypes; genes that correlate with differential brain images - Methods of assigning quantitative phenotype measurement summary statistics to differential brain image information associated with neuropsychiatric disorders are provided. Summary statistics are correlated to genotype information to identify loci that correlate with differential brain image phenotypes. Methods of identifying modulators of genes at the loci are provided, as well as modulators identified by the methods. Systems for correlating polymorphisms and differential brain image phenotypes, for identifying modulators and for making correlations between differential brain activation phenotypes and genotypes are also provided | 10-02-2008 |
| 20080241840 | Methods of detection using immuno-Q-Amp technology - The present invention describes, in certain embodiments, a composition for detecting a tau protein comprising a modified detector molecule having two ends, a first end capable of binding the tau protein and a second end comprising a single-stranded DNA template, wherein the template is capable of being replicated by an RNA polymerase. | 10-02-2008 |
| 20080241841 | Method and apparatus for sample preparation - A method of the present invention comprises fractionating a sample solution containing analyte DNA molecules into small droplets, wherein the number M of the droplets is greater than the total number N of the DNA molecules, subjecting an emulsion containing the droplets to, for example, PCR amplification, and detecting the presence or absence (amount) of an amplicon obtained in each droplet by fluorescent detection using an intercalator or the like. | 10-02-2008 |
| 20080241842 | Gene Methylation as a Biomarker in Sputum - The present invention provides for a method to monitor the health of a subject. The method includes obtaining a test sample from the patient. A first probe specific for a CpG promoter region of a biomarker selected from p16, MGMT, PAX-α, PAX5-β, RASSF1A, HLHP, GATA4, GATA5, SFRP1, LAMC2, IGFBP3, H-cadherin, BETA 3, HLHP, and DAPK is provided to the sample. The probe contacts the test sample. The DNA of interest from the test sample is isolated. A second stage probe specific for a second CpG promoter region of a biomarker selected from p16, MGMT, PAX-α, PAX5-β, RASSF1A, HLHP, GATA4, GATA5, SFRP1, LAMC2, IGFBP3, H-cadherin, BETA 3, HLHP, and DAPK is provided to the sample to form a second stage PCR product. The DNA is analyzed for hypermethylation of the promoter region for at least one of p16, MGMT, PAX-α, PAX5-β, RASSF1A, HLHP, GATA4, GATA5, SFRP1, LAMC2, IGFBP3, H-cadherin, BETA 3, HLHP, and DAPK. Hypermethylation of the promoter region of at least one of p16, MGMT, PAX-α, PAX5-β, RASSF1A, HLHP, GATA4, GATA5, SFRP1, LAMC2, IGFBP3, H-cadherin, BETA 3, HLHP, and DAPK is an indication that the subject is at increased risk of developing cancer for example, non small cell lung cancer. | 10-02-2008 |
| 20080241843 | Single-cell analysis systems, methods of counting molecules in a single-cell, cylindrical fluorescence detection systems - Embodiments of the present disclosure provide for single-cell analysis systems, methods of detecting target components in a single cell, cylindrical fluorescence detection systems, and the like. | 10-02-2008 |
| 20080241844 | Devices and Methods for the Performance of Miniaturized In Vitro Assays - This invention relates to methods and apparatus for performing microanalytic and microsynthetic analyses and procedures. The invention specifically provides devices and methods for performing miniaturized in vitro assays on biological samples, such as the polymerase chain reaction and Sanger sequencing reactions. Methods specific for the apparatus of the invention for performing PCR are provided. | 10-02-2008 |
| 20080241845 | Method of Extracting Chromatin Fractions From Intact Cells - Methods are provided for isolation of chromatin fractions of nucleoproteins containing histone H1, H2A, H2B, H3 and H4 proteins and/or histone H1, H2A, H2B, H3 and/or H4 proteins, from intact cells. The methods preserve original patterns of covalent modifications of the histone proteins. | 10-02-2008 |
| 20080241846 | Genetic polymorphisms associated with coronary events and drung response, methods of detection and uses thereof - The present invention provides compositions and methods based on genetic polymorphisms that are associated with coronary heart disease (particularly myocardial infarction), aneurysm/dissection, and/or response to drug treatment, particularly statin treatment. For example, the present invention relates to nucleic acid molecules containing the polymorphisms, variant proteins encoded by these nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and variant proteins, and methods of using the nucleic acid molecules and proteins as well as methods of using reagents for their detection. | 10-02-2008 |
| 20080241847 | METHOD AND APPARATUS FOR IN VIVO SURVEILLANCE OF CIRCULATING BIOLOGICAL COMPONENTS - The invention relates generally to in vivo collection of circulating molecules, tumor cells and other biological markers using a collecting probe. The probe is configured for placement within a living organism for an extended period of time to provide sufficient yield of biological marker for analysis. | 10-02-2008 |
| 20080241848 | METHODS FOR PRENATAL DIAGNOSIS OF ANEUPLOIDY - Methods are disclosed for the automated prenatal genetic diagnosis of aneuploidy using an automated fluorescence microscope, conducted on samples of maternal blood that have been hybridized with FISH probes. | 10-02-2008 |
| 20080241849 | Methods and compositions for diagnosing epithelial cell cancer - Provided is a method for detecting metastases of epithelial cancers, comprising detecting in non-primary tissue overexpression of a nucleic acid of KS1/4, or detecting in non-primary tissue overexpression of a combination of nucleic acids of KS1/4 and PIP, of nucleic acids of KS1/4 and mam, of nucleic acids of PIP and mam, of nucleic acids of KS1/4, PIP and mam, or of nucleic acids of KS1/4 and lunx, the overexpression of a nucleic acid of KS1/4, or the overexpression of a combination of nucleic acids of KS1/4 and PIP, of nucleic acids of KS1/4 and mam, of nucleic acids of PIP and mam, of nucleic acids of KS1/4, PIP and mam, or of nucleic acids of KS1/4 and lunx in non-primary tissue being correlated with metastases of epithelial cancers. | 10-02-2008 |
| 20080248462 | Diagnostics and Therapeutics for Diseases Associated with Arginyl Aminopeptidase (Aminopeptidase B)-Like 1 (Rnpepl1) - The invention provides a human RNPEPL1 which is associated with the cardiovascular diseases, endocrinological diseases, metabolic diseases, cancer, inflammation, gastroenterological diseases, hematological diseases, respiratory diseases, neurological diseases, urological diseases and reproduction disorders. The invention also provides assays for the identification of compounds useful in the treatment or prevention of cardiovascular diseases, endocrinological diseases, metabolic diseases, cancer, inflammation, gastroenterological diseases, hematological diseases, respiratory diseases, neurological diseases, urological diseases and reproduction disorders. The invention also features compounds which bind to and/or activate or inhibit the activity of RNPEPL1 as well as pharmaceutical compositions comprising such compounds. | 10-09-2008 |
| 20080248463 | Split Enzyme Linked Immunosorbent and Nucleic Acid Assays - A method for detecting the presence of an analyte in a solution. The analyte includes at least two mutually exclusive recognition sites that are capable of binding to corresponding recognition molecules. Biosensors are provided that include the recognition molecules, which are attached to the inactive portions of a split enzyme. The recognition sites are located such that the inactive enzyme portions combine to form a detectable biologically active enzyme when the recognition molecules bind to recognition sites. | 10-09-2008 |
| 20080248464 | Method of DNA Shuffling with Polynucleotides Produced by Blocking or Interrupting a Synthesis or Amplification Process - Disclosed is a process of performing “sexual” PCR which includes generating random polynucleotides by interrupting or blocking a synthesis or amplification process to show or halt synthesis or amplification of at least one polynucleotide, optionally amplifying the polynucleotides, and reannealing the polynucleotides to produce random mutant polynucleotides. Also provided are vector and expression vehicles including such mutant polynucleotides, polypeptides expressed by the mutant polynucleotides and a method for producing random mutant polypeptides. | 10-09-2008 |
| 20080248465 | Polymorphisms in the Fcgr2b Promoter and Uses Thereof - The invention relates to the FCGR2B gene and its promoter. In particular, the invention relates to FCGR2B promoters with specific nucleotides at polymorphic sites. Characterization of the nucleotides at polymorphic sites is useful for characterizing the gene and the protein and is useful for determining predisposition or susceptibility to certain diseases and infections in a subject or a population of subjects. Such characterization of the gene or protein is also useful for determining immunoresponsiveness or responsiveness to therapeutic agents in a subject or population of subjects. Thus, disclosed herein are a variety of related nucleic acids, methods and tools. | 10-09-2008 |
| 20080248466 | Method Of Detecting Mutations In The Gene Encoding Cytochrome P450-2C19 - The present invention describes a method for the simultaneous identification of two or more mutations located in the gene encoding Cytochrome P450-2C19. Multiplex detection is accomplished using multiplexed tagged allele specific primer extension (ASPE) and hybridization of such extended primers to a probe, preferably an addressable anti-tagged support. | 10-09-2008 |
| 20080248467 | System for pulling out regulatory elements using yeast - Disclosed are methods for identifying molecular interactions between DNA sequences and proteins in vivo. The methods of the invention employ known or suspected DNA-binding proteins and genomic DNA in a plasmid library. Interacting molecules direct the expression of a reporter gene, the expression of which is then assayed. Also disclosed are genetic constructs useful in practicing the methods of the invention. | 10-09-2008 |
| 20080248468 | Method for Improved Selection of Rnai Transfectants - The present invention is directed to a method for inactivation of expression of a gene in a eucaryotic cell comprising (i) transfection of a eucaryotic cell with DNA comprising an expression cassette for expression of a cell surface protein and an expression cassette for expression of a RNAi compound, said compound being capable of inactivating expression of said gene, wherein said expression cassette for expression of a cell surface protein and said expression cassette for expression of a RNAi compound are located on the same vector DNA, and (ii) enrichment and/or selection of cells which express said cell surface protein. | 10-09-2008 |
| 20080248469 | Methods for Identifying Nucleotides of Interest in Target Polynucleotides - In some embodiments, the present teachings provide a method of identifying a nucleotide of interest in a target polynucleotide. In some embodiments, the method can comprise forming an amplification strand, wherein the amplification strand comprises a hairpinning end region; hybridizing the hairpinning end region of the amplification strand with a hairpinning region of a target polynucleotide, to form a self-complementary amplification product. After performing an extension reaction, wherein the hairpinning end region of the amplification strand is extended, an extended reaction product is formed. Detection of the extended reaction product can result in the identification of a nucleotide of interest in the target polynucleotide. Additional methods, as well as kits, are also provided. | 10-09-2008 |
| 20080248470 | GENETIC SCREENING FOR PREDICTING ANTIDEPRESSANT DRUG RESPONSE BASED ON THE MONOAMINE TRANSPORTER GENE POLYMORPHISM COMBINATION - The present invention discloses is a method of selecting a drug based on personal genetic information when prescribing an antidepressant for a depressed patient. According to the invention, either a noradrenaline reuptake inhibitor (NRI) antidepressant or a selective serotonin reuptake inhibitor (SSRI) antidepressant can be selected based on the combination of monoamine transporter gene polymorphisms, that is, NET G1287A polymorphism in a norepinephrine transporter (NET) gene, serotonin transporter gene (5-HTT) promoter polymorphism and 5-HTT gene intron 2 polymorphism. Also, based on the norepinephrine transporter gene NET G1287A polymorphism alone, the noradrenaline reuptake inhibitor (NRI) antidepressant can be selected. | 10-09-2008 |
| 20080248471 | Methods for disease detection - The present invention provides methods for detecting disease by analysis of a patient sample to determine the integrity of nucleic acids in the sample. | 10-09-2008 |
| 20080248472 | Method and Kit for Determination of Thymidine Kinase Activity and Use Thereof - A method and assay kit for determination of thymidine kinase (TK) activity in a biological sample, such as blood, serum, plasma, Cerebral Spinal Fluid (CSF), pleural fluid, ascites, tissues, cells and extracts thereof, is described. The method comprises contacting, in a buffer, a Basic Reaction Mixture comprising: solid surface-attached primer and/or template, a modified deoxy nucleoside, such as BromodeoxyUridine, IododeoxyUridine, Fluorodeoxy-Uridine or VinyldexoyThymidine as a kinase enzyme substrate, a phosphate donor, a nucleotide polymerizing enzyme, and a kinase enzyme source devoid of TK activity, such as a yeast extract, with the biological sample. After incubation the amount of modified deoxy nucleoside that has been incorporated into the solid surface-attached primer and/or template, is determined and the TK activity present in the biological sample is directly proportional to the amount of incorporated modified deoxy nucleoside. The method and assay kit are useful in the diagnosing, prognosis monitoring of disease progression and treatment effects of cell-proliferation disorders or diseases, such as cancer, and in the screening of compounds, e.g. new drug candidates, affecting enzymatic pathways, which may obstruct the formation of thymidine phosphates or interfere with nucleic acid synthesis. | 10-09-2008 |
| 20080248473 | Method and Kit for the Detection of Bacterial Species by Means of Dna Analysis - The present invention relates to the detection and identification of different bacterial species, all of which cause zoonosis, based on DNA analysis. More specifically, the invention provides the primers, probes, genes and genic regions required to apply a method for the simultaneous detection of bacteria and bacterial groups belonging to the genera | 10-09-2008 |
| 20080248474 | AMPLIFICATION METHODS - Methods are provided for amplification and monitoring of oligonucleotide amplification in which a primer has an overlap with one or more bases of a detection probe. | 10-09-2008 |
| 20080248475 | Methods and Compositions for Modulating Gluconeogenesis Using PGC-1 - The invention provides novel methods and compositions for modulating gluconeogenesis through modulation of PGC-1 activity or expression. Also provided are methods for identifying compounds that modulate gluconeogenesis through modulation of PGC-1 activity or expression, as well as methods for identifying compounds that modulate the interaction of PGC-1 with PGC-1 target molecules. Further provided are methods for treating disorders characterized by aberrant gluconeogenesis. | 10-09-2008 |
| 20080248476 | Genetic polymorphisms associated with myocardial infarction, methods of detection and uses thereof - The present invention is based on the discovery of genetic polymorphisms that are associated with myocardial infarction. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, variant proteins encoded by such nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and proteins, and methods of using the nucleic acid and proteins as well as methods of using reagents for their detection. | 10-09-2008 |
| 20080248477 | Novel ITALY, LOR-2, STRIFE, TRASH, BDSF, LRSG, and STMST protein and nucleic acid molecules and uses therefor - Novel ITALY, LOR-2, STRIFE, TRASH, BDSF, LRSG, and STMST polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length ITALY, LOR-2, STRIFE, TRASH, BDSF, LRSG, and STMST proteins, the invention further provides isolated ITALY, LOR-2, STRIFE, TRASH, BDSF, LRSG, and STMST fusion proteins, antigenic peptides and anti-ITALY, LOR-2, STRIFE, TRASH, BDSF, LRSG, and STMST antibodies. The invention also provides ITALY, LOR-2, STRIFE, TRASH, BDSF, LRSG, and STMST nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals in which an ITALY, LOR-2, STRIFE, TRASH, BDSF, LRSG, and STMST gene has been introduced or disrupted. Diagnostic, screening and therapeutic methods utilizing compositions of the invention are also provided. | 10-09-2008 |
| 20080248478 | MOLECULAR HISTOLOGICAL ANALYSIS OF MULTICELLULAR SAMPLES - The present invention provides modified digital images of a multicellular sample using molecular profiles of cells from said multicellular sample for a molecular histological analysis. | 10-09-2008 |
| 20080248479 | Methods for Isolating and Characterizing Endogenous mRNA-Protein (mRNP) Complexes - Cellular mRNA-protein (mRNP) complexes are partitioned in vivo by contacting a biological sample with at least one ligand that specifically binds at least one component of a mRNP complex. Suitable biological samples comprise at least one mRNA-protein (mRNP) complex and include cell cultures, cell extracts, and whole tissue, including tumor tissue. Ligands include antibodies that specifically bind RNA-binding or RNA-associated proteins present in the mRNP complex. The mRNP complex is separated by binding the ligand with a binding molecule specific for the ligand, where the binding molecule is attached to a solid support. The mRNP complex is collected by removing the mRNP complex from the solid support. After collecting the mRNP complex, the mRNA bound within the complex may be characterized and identified. Subsets of the total mRNA population of a cell may accordingly be characterized, and a gene expression profile of the cell obtained. | 10-09-2008 |
| 20080248480 | Novel nucleotide mixture for improved nucleic acid amplification performance - The present invention relates to modification of amplification buffer used in amplification reactions. The modifications result in a significant improvement in results of amplification. In particular, the present invention provides methods and buffers for performing an amplification reaction utilizing a buffer comprising nucleotide triphosphates comprising treating the buffer to substitute a portion of the nucleotide triphosphates with nucleotide diphosphates. | 10-09-2008 |
| 20080248481 | METHODS OF EVALUATING CELLS AND CELL CULTURES - Methods of evaluating the composition of a cell culture (e.g., to distinguish chondrocytes from fibroblasts) and methods for evaluating the phenotype of an individual cell (e.g., as a chondrocyte) are disclosed. The methods may be used, for example, for assessing chondrocyte cultures used for treatment of cartilage defects. In some embodiments, the invention involves identifying cell culture composition or the identity of a cell based on expression level of a fibroblast marker. In other embodiments, the invention involves comparing expression levels of at least one chondrocyte marker and at least one fibroblast marker in a cell culture sample or in an individual cell. In illustrative embodiments, the chondrocyte marker is hyaluronan and proteoglycan link protein 1 (HAPLN1), and the fibroblast marker is microfibrillar associated protein 5 (MFAP5). | 10-09-2008 |
| 20080248482 | BIOMARKERS FOR TOXIC ALGAE - The present invention is directed toward biomarkers that identify characteristics of algae. The invention is further directed toward biomarkers that serve to identify algae species and strains of algae species as well as detect the presence of algal toxins. Additional embodiments feature methods utilizing algal biomarkers and polypeptides that can serve as biomarkers. | 10-09-2008 |
| 20080248483 | METHODS OF IDENTIFYING THERAPEUTIC COMPOUNDS IN A GENETICALLY DEFINED SETTING - The invention provides a method of identifying therapeutic compounds in a genetically defined setting. The method consists of contacting a cell indicative of a pathological condition from a diseased individual and a cell from a genetically related normal individual with a plurality of candidate therapeutic compounds under suitable assay conditions, and identifying a compound that preferentially alters a predetermined property of the cell from the diseased individual. | 10-09-2008 |
| 20080248484 | METHOD FOR THE EARLY DETECTION OF PANCREATIC CANCER AND OTHER GASTROINTESTINAL DISEASE CONDITIONS - The present invention uses peripheral blood monocyte-lymphocyte for the early diagnosis of pancreatic cancer, as well as other conditions of the pancreas and other organs. The peripheral blood lymphocytes recognize the new neoplasm in the pancreas, as well as disease processes in other organ systems. The evaluation of this specific recognition of the disease process by the peripheral blood monocyte-lymphocyte through gene microarray expression patterns constitute a successful method for the early detection of pancreatic cancer and other organ disease processes. This document describes the process used in this method of early diagnosis. | 10-09-2008 |
| 20080254445 | Prognosis in Cancer Patients Vaccinated with a Cancer Antigen Peptide-Associated Agent - The correlation of clinical benefits and immune responses to peptides in cancer patients who were vaccinated with CTL-directed peptides is shown. The invention relates to a process for determining prognosis in a cancer patient vaccinated with a cancer antigen peptide-associated agent, which comprises measuring a level of an antibody specific to the cancer antigen peptide, and assessing whether the level is significantly increased as compared to the level at pre-vaccination. | 10-16-2008 |
| 20080254446 | Method of Detecting Target Molecule by Using Aptamer - An aptamer-probe complex for detecting the presence of a target molecule is disclosed. The complex of the present invention contains an aptamer moiety which is able to bind to an indicator protein and change the properties of the indicator protein, and a probe moiety which is able to bind to a target molecule, wherein the aptamer moiety and the probe moiety are combined in such a manner that the binding mode between the aptamer moiety and the indicator protein changes when the probe moiety binds to the target molecule. A target molecule can be detected with combination of an aptamer which binds to a certain protein, and a probe which binds to the target molecule, utilizing the properties of that protein as an indicator. | 10-16-2008 |
| 20080254447 | Method and Nucleic Acids for the Improved Treatment of Breast Cell Proliferative Disorders - The present invention relates to modified and genomic sequences, to oligonucleotides and/or PNA-oligomers for detecting the cytosine methylation state of genomic DNA, as well as to a method for predicting the disease free survival and/or response of a subject with a cell proliferative disorder of the breast tissues, to endocrine treatment. | 10-16-2008 |
| 20080254448 | Analysis Chip With Reference Range, Kits and Methods of Analysis - Analysis chip of at least one analyte, said chip comprising at least one analysis spot for recognition and immobilization specific to the analyte; and a reference range (G) comprising several reference spots each arranged on said chip in a defined manner and independently of one another, each reference spot of this range comprising, immobilized on its surface and in a defined proportion P that is different and known for each spot relative to the other reference spots of said range: a probe reference molecule (PRM) permitting recognition and hybridization specifically to a defined target reference molecule (TRM), and/or an inert oligonucleotide molecule (IM) incapable of recognition and hybridization with said PRM, these molecules both being unable to immobilize said analyte or analytes. The method of the invention comprises the use of said chip with its reference range for analysis of said, at least one, analyte. | 10-16-2008 |
| 20080254449 | Polynucleotides For the Detection of Escherichia Coli 0157 - Polynucleotide primers and probes for the amplification and detection of | 10-16-2008 |
| 20080254450 | Human Obesity Susceptibility Genes Encoding Peptide Hormones and Uses Thereof - The present invention discloses the identification of human obesity susceptibility genes, which can be used for the diagnosis and prevention of obesity and related disorders. The invention more specifically discloses that the PPY and PYY gene on chromosome 17 and certain alleles thereof are related to susceptibility to obesity. The present invention relates to particular mutations in the PPY and PYY gene and expression products, as well as to diagnostic tools and kits based on these mutations. The invention can be used in the diagnosis of predisposition to or protection from, detection, prevention and/or treatment of coronary heart disease and metabolic disorders, including hypoalphalipoproteinemia, familial combined hyperlipidemia, insulin resistant syndrome X or multiple metabolic disorder, coronary artery disease, diabetes and dyslipidemia. | 10-16-2008 |
| 20080254451 | Compositions and Methods for Treating Schizophrenia and Related Disorders - The present invention relates, generally, to methods and compositions for detecting or treating mental disorders, such as schizophrenia. The present invention more particularly discloses the identification of human genes which can be used for the diagnosis, prevention and treatment of schizophrenia and related disorders, as well as for the screening of therapeutically active drugs. The invention further discloses specific polymorphisms or alleles of the CNTFR gene that are related to schizophrenia, as well as diagnostic tools and kits based on these markers. The invention can be used in the diagnosis of or predisposition to, detection, prevention and/or treatment of schizophrenia and related disorders. | 10-16-2008 |
| 20080254452 | Pkp3 Oncogene as a Prognostic Indicator for Lung Cancer - The present invention provides the method of predicting an non-small cell lung cancer (NSCLC) prognosis. | 10-16-2008 |
| 20080254453 | Analysis of methylation using selective adaptor ligation - Methods of analyzing DNA to identify regions of the genome that are methylated in a genomic sample are disclosed. In one aspect genomic DNA is fragmented using a restriction enzyme with a degenerate recognition site, methylated restriction fragments are separated from unmethylated fragments by affinity purification. The complexity of the methylated fragments is reduced by amplification of a subset of the fragments using adaptors that ligate to a subset of the fragments. The amplified product is fragmented, labeled and hybridized to an array of probes. The hybridization pattern is analyzed to determine methylation status of cytosines. | 10-16-2008 |
| 20080254454 | GENETIC MARKERS FOR ASSESSING RISK OF PREMATURE BIRTH RESULTING FROM PRETERM PREMATURE RUPTURE OF MEMBRANES - A method to identify women who are at risk for preterm delivery due to premature rupture of membranes (PPROM) is provided. The method entails detecting the presence of SERPINH1 gene variants that express low levels of the gene product, heat shock protein Hsp47. The occurrence of a T (rather than C) at a single nucleotide polymorphism (SNP) site at position −656 of the SERPINH1 gene promoter, together with the absence of a 12 base pair deletion at positions −694 to −683 of the promoter, result in an increased risk of PPROM. The method enables medical professionals to identify those at risk, and to provide suitable therapeutic intervention. | 10-16-2008 |
| 20080254455 | DETECTING PROSTATE CANCER - Methods and kits for detecting prostate cancer in urine samples include detecting the methylation status of various genes. | 10-16-2008 |
| 20080254456 | GENE MARKER FOR HUMAN HEPATOCELLULAR CARCINOMA DIAGNOSIS - The present invention relates to a gene marker for diagnosis of human hepatocellular carcinoma (HCC), which is selected from the group consisting of IGF2, VEGF, MET, SUMO2, CDK4, MMP9, PLK1, AFP, Rb1, CYP3A4, LAP3, RIZ, DLC1, ITIH1, FetB, ALDOB, SULT2A1, ASS, HP, SERIND1, EI24, HGD, RODH, F2, SORD, and KNG. The HCC is diagnosed effectively and efficiently based on detecting the expression levels of the present gene marker from the liver tissue sample of an individual to be diagnosed. | 10-16-2008 |
| 20080254457 | Methods for Evaluating Drug-Resistance Gene Expression in the Cancer Patient - The methods of the invention detect in a qualitative or quantitative fashion drug-resistance RNA and DNA in blood plasma, serum, and other bodily fluids. The methods of the invention thereby enable the assessment of drug resistance in a neoplasm without the requirement of a tissue biopsy. The inventive methods are useful for the evaluation, monitoring, and selecting of drug treatment regimens, and for determining a predisposition for or prognosis of chemoresistant neoplastic disease. | 10-16-2008 |
| 20080254458 | Method of pre-treating in pleural effusion for detection of Mycobacterium tuberculosis - The present invention provides a method for preparing nucleic acid from a sample for detecting | 10-16-2008 |
| 20080254459 | Methods and Biomarkers for Detecting Nanoparticle Exposure - Methods for gene expression profiling for exposure to nanoscale particulates or nanomaterials is provided together with identified biomarkers for nanomaterial exposure. A toxicogenomic exposure profile for nanomaterial contact is provided in accordance with a systems biology approach by iteratively sampling a test system several times after contact with nanomaterials of various chemical types. | 10-16-2008 |
| 20080254460 | Method of Fetal Cell Enrichment - The present invention provides a method of enriching fetal cells in a maternal blood sample wherein cells which are CD34+ and capable of adhering to a solid support are selected, an analysable sample of fetal cells obtainable by the methods of the invention and a kit for use in the methods of the invention. There is also provided a method of fetal gender determination and a method of diagnosing a fetal genetic abnormality. | 10-16-2008 |
| 20080254461 | METHODS FOR ISOLATING AND CHARACTERIZING ENDOGENOUS mRNA-PROTEIN (mRNP) COMPLEXES - Cellular mRNA-protein (mRNP) complexes are partitioned in vivo by contacting a biological sample with at least one ligand that specifically binds at least one component of a mRNP complex. Suitable biological samples comprise at least one mRNA-protein (mRNP) complex and include cell cultures, cell extracts, and whole tissue, including tumor tissue. Ligands include antibodies that specifically bind RNA-binding or RNA-associated proteins present in the mRNP complex. The mRNP complex is separated by binding the ligand with a binding molecule specific for the ligand, where the binding molecule is attached to a solid support. The mRNP complex is collected by removing the mRNP complex from the solid support. After collecting the mRNP complex, the mRNA bound within the complex may be characterized and identified. Subsets of the total mRNA population of a cell may accordingly be characterized, and a gene expression profile of the cell obtained. | 10-16-2008 |
| 20080254462 | ALTERED ZDHHC8 EXPRESSION AS A MARKER OF INCREASED RISK OF SCHIZOPHRENIA - The present invention relates to the role of mutations that perturb expression of ZDHHC8 in increased susceptibility to schizophrenia. It is based, at least in part, on the discovery of a mechanism by which a high risk allele disrupts expression of functional ZDHHC 8, and on the discovery that two proteins involved in synaptic pathways associated with schizophrenia interact with ZDHHC8, at least one of which is a substrate for that enzyme. | 10-16-2008 |
| 20080254463 | DETECTION METHOD FOR DIFFERENTIATING BETWEEN CHICKEN-DERIVED INGREDIENTS AND EGG-DERIVED INGREDIENTS IN PRODUCTS - The present invention relates to a detection method to differentiate between egg-derived ingredients and chicken-derived ingredients (chicken parts/tissues, excluding eggs) in foods or other products and primer pairs and probes used for specifically detecting chicken in foods or products. | 10-16-2008 |
| 20080254464 | Identification of genetic markers of biological age and metabolism - A method of measuring the biological age of a multicellular organism is disclosed. In one embodiment this method comprises the steps of obtaining a sample of nucleic acid isolated from the organism's organ, tissue or cell and determining the expression pattern of a panel of sequences within the nucleic acid that have been predetermined by either increase or decrease in response to biological aging of the organ, tissue or cell. A method of obtaining biomarkers of aging is also disclosed. This method comprises the step of comparing a gene expression profile of a young multicellular organism subject's organ, tissue or cells; a gene expression profile from a chronologically aged subject's organ, tissue or cell; and a gene expression profile from a chronologically aged but biologically younger subject's organ, tissue or cell and identifying gene expression alterations that are observed when comparing the young subjects and the chronologically aged subjects and are not observed or reduced in magnitude when comparing the young subjects and the chronologically aged but biologically younger subjects. | 10-16-2008 |
| 20080254465 | Assays for measuring nucleic acid binding proteins and enzyme activities - Processes for measuring DNA or RNA binding proteins, specific nucleic acids, as well as enzyme activities using labeled nucleic acids of labeled protein/peptide molecules are provided. | 10-16-2008 |
| 20080254466 | Heregulin-like Factor - The present invention relates to a novel HLF protein which is a member of the heregulin family. In particular, isolated nucleic acid molecules are provided encoding the human HLF protein. HLF polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of HLF activity. Also provided are diagnostic methods for detecting disorders of the regulation of cell growth and therapeutic methods for treating disorders of the regulation of cell growth. | 10-16-2008 |
| 20080254467 | Automated High-Throughput Flow-Through Real-Time Diagnostic System - An automated real-time flow-through system capable of processing multiple samples in an asynchronous, simultaneous, and parallel fashion for nucleic acid extraction and purification, followed by assay assembly, genetic amplification, multiplex detection, analysis, and decontamination. The system is able to hold and access an unlimited number of fluorescent reagents that may be used to screen samples for the presence of specific sequences. The apparatus works by associating extracted and purified sample with a series of reagent plugs that have been formed in a flow channel and delivered to a flow-through real-time amplification detector that has a multiplicity of optical windows, to which the sample-reagent plugs are placed in an operative position. The diagnostic apparatus includes sample multi-position valves, a master sample multi-position valve, a master reagent multi-position valve, reagent multi-position valves, and an optical amplification/detection system. | 10-16-2008 |
| 20080254468 | Micro-Fluidic Temperature Driven Valve - Subject of the present invention is a micro-fluidic device for the use in an apparatus for analyzing a liquid sample by nucleic acid amplification, an apparatus for analyzing a liquid sample by nucleic acid amplification, a method for analyzing a liquid sample and a method for amplifying nucleic acids in a liquid. | 10-16-2008 |
| 20080254469 | Method for Regenerating Hydrophilic and Osteophilic Surface of an Implant - Described herein are methods for testing an aged surface on an implant, methods for regenerating a hydrophilic and osteophilic surface on the implant and kits therefor. | 10-16-2008 |
| 20080254470 | Methods and Nucleic Acids For the Analysis of Gene Expression Associated With the Prognosis of Cell Proliferative Disorders - The present application provides methods and nucleic acids for providing a prognosis of cell proliferative disorders, most preferably cancer but not breast cancer. | 10-16-2008 |
| 20080254471 | Apparatus and Method for Microbial and Forensic Sampling and Manipulation - The present invention provides a sterile collection package and methods for sample procurement and manipulation including microbial and forensic testing. The collection package comprises a tissue or sample collection container with a sealable opening, at least one drain container, and at least one tubing set that connects the tissue or sample collection container and the drain container, the tubing set further optionally comprising a sampling hub for withdrawing sample fluid for microbial and forensic testing. Samples, such as donated tissues, diseased tissues and article and tissues for forensic analysis are mixed with a sampling solution in the collection container and aliquots of the sampling solution are withdrawn through the sampling hub for microbial and/or forensic testing. | 10-16-2008 |
| 20080254472 | METHOD FOR DETECTING NUCLEIC ACID IN SAMPLE, METHOD FOR DESIGNING PROBES, SYSTEM FOR DESIGNING PROBES THEREFOR - Accurate detection of a target microorganism from a sample, which may contain a plurality of microorganisms, is intended. This can be realized by eliminating a possibility of cross-hybridization of a microorganism with other probes, which can accelerate the designing speed, detecting microorganisms one-by-one in the designed order, and absorbing all the existing microorganisms. | 10-16-2008 |
| 20080254473 | PREDICTING POST-TREATMENT SURVIVAL IN CANCER PATIENTS WITH MICRORNAS - This invention provides a method for predicting the post-treatment survival prospect of a cancer patient based on the expression level(s) of microRNAs hsa-miR137, hsa-miR372, hsa-miR182*, hsa-miR221, and hsa-let-7a in that cancer patient. | 10-16-2008 |
| 20080254474 | DNA METHYLATION ANALYSIS BY DIGITAL BISULFITE GENOMIC SEQUENCING AND DIGITAL METHYLIGHT - Provided are novel sensitive methylation assays referred to herein as Digital MethyLight, comprising stochastically distributing and compartmentalizing bisulfite-treated genomic DNA over multiple PCR reaction wells for detection of individually methylated DNA molecules in a large background of unmethylated DNA. Digital Bisulfite Genomic DNA Sequencing methods are also provided for high-resolution DNA methylation information without subcloning. Background signal and PCR contaminants are diluted, while the ratio of primer to methylated template DNA is kept high. Preferably, biological fluid (e.g., urine, blood-based (e.g., plasma and/or serum)) samples are analyzed for cancer diagnosis, prognosis and surveillance. Multiplexed PCR formats may be implemented to enhance when using small DNA amounts. Compositions and methods for diagnosis and/or prognosis of breast cancer, comprising the use of FOXE1, CLDN5 and/or RUNX3 gene markers are also provided (SEQ ID NOS: 17, 16 and 18, respectively for respective CpG island sequences), and in preferred embodiments plasma or serum samples are used. | 10-16-2008 |
| 20080254475 | NEMATODES AS MODEL ORGANISMS FOR THE INVESTIGATION OF NEURODEGENERATIVE DISEASES, IN PARTICULAR PARKINSONS DISEASE, USES AND METHODS FOR THE DISCOVERY OF SUBSTANCES AND GENES WHICH CAN USED IN THE TREATMENT OF THE ABOVE DISEASE STATES AND IDENTIFICATION OF A NEMATODE GENE - The invention relates to nematodes as model organisms for the investigation of neurodegenerative diseases, in particular, Parkinsons disease, uses and methods for the discovery of substances and genes which can be used in the treatment of the above disease states and identification of a nematode gene, From | 10-16-2008 |
| 20080254476 | IL-1 GENE CLUSTER AND ASSOCIATED INFLAMMATORY POLYMORPHISMS AND HAPLOTYPES - The invention provides methods and compositions relating to identification and use of genetic information from the IL-1 gene cluster—including the structure and organization of novel IL-1-like genes found within the IL-1 locus as well as polymorphisms and associated haplotypes within these genes. The invention thereby expands the repertoire of useful genetic information available from the IL-1 locus—which contains the previously-identified IL-1α, IL-1β and IL-1RN genes, for predicting IL-1 associated phenotypes (e.g. increased or decreased risks of inflammatory disease) and for treating IL-1 haplotype associated inflammatory phenotypes. | 10-16-2008 |
| 20080254477 | IL-1 GENE CLUSTER AND ASSOCIATED INFLAMMATORY POLYMORPHISMS AND HAPLOTYPES - The invention provides methods and compositions relating to identification and use of genetic information from the IL-1 gene cluster—including the structure and organization of novel IL-1-like genes found within the IL-1 locus as well as polymorphisms and associated haplotypes within these genes. The invention thereby expands the repertoire of useful genetic information available from the IL-1 locus—which contains the previously-identified IL-1α, IL-1β and IL-1RN genes, for predicting IL-1 associated phenotypes (e.g. increased or decreased risks of inflammatory disease) and for treating IL-1 haplotype associated inflammatory phenotypes. | 10-16-2008 |
| 20080254478 | IL-GENE CLUSTER AND ASSOCIATED INFLAMMATORY POLYMORPHISMS AND HAPLOTYPES - The invention provides methods and compositions relating to identification and use of genetic information from the IL-1 gene cluster—including the structure and organization of novel IL-1-like genes found within the IL-1 locus as well as polymorphisms and associated haplotypes within these genes. The invention thereby expands the repertoire of useful genetic information available from the IL-1 locus—which contains the previously-identified IL-1α, IL-1β and IL-1RN genes, for predicting IL-1 associated phenotypes (e.g. increased or decreased risks of inflammatory disease) and for treating IL-1 haplotype associated inflammatory phenotypes. | 10-16-2008 |
| 20080261204 | Polynucleotide Ligation Reactions - The method of the invention is useful in quantifying the absolute or relative number of unique molecules present in a sample after carrying out an analysis procedure on the sample, and comprises the steps of: (i) attaching a unique molecular tag to substantially all of the molecules in the sample; (ii) carrying out the analysis procedure using the molecules of the sample; and (iii) on the basis of the molecular tags determining the absolute or relative number of unique molecules present in the original sample which underwent the analysis procedure. | 10-23-2008 |
| 20080261205 | Method for the Simultaneous Determination of Blood Group and Platelet Antigen Genotypes - RBC and platelet (Plt) alloimmunization requires antigen-matched blood to avoid adverse transfusion reactions. Some blood collection facilities use unregulated Abs to reduce the cost of mass screening, and later confirm the phenotype with government approved reagents. Alternatively, RBC and Plt antigens can be screened by virtue of their associated single nucleotide polymorphisms (SNPs). We developed a multiplex PCR-oligonucleotide extension assay using the GenomeLab SNPStream platform to genotype blood for a plurality of blood group antigen-associated SNPs, including but not limited to: RhD (2), RhC/c, RhE/e, S/s, K/k, Kp | 10-23-2008 |
| 20080261206 | Oligonucleotide for Detection of a Microorganism, Diagnostic Kits and Methods for Detection of Microorganisms Using the Oligonucleotide - The present invention relates to a method so called Bacterial Digitalcode System (BaDis) that identifies microorganism by using bacterial-specific, genus-specific and species-specific oligonucleotides from a variety of samples or specimens for detection and differential diagnosis of microorganism. Particularly, the present invention relates to bacterial-specific, genus-specific and species-specific oligonucleotides designed by the target nucleotide sequences of 23S rDNA or ITS gene, polymerase chain reaction (hereinafter, referred to as “PCR”) kits using the oligonucleotides as a primer, the microarray containing the oligonucleotides as a probe, and methods for detecting microorganism by using the oligonucleotides. Therefore, the present invention can be applied to detect the presence of microorganism and diagnose differentially all microorganism such as pathogenic bacteria of infectious diseases, bacteria inducing food poisoning, bacteria contaminating biomedical products and environmental pollutants. | 10-23-2008 |
| 20080261207 | Method of Measuring Cancer Susceptibility - An individual's susceptibility to cancer is assessed based on the individual's cellular response to mutagenic agents such as radiation. The level of a growth-suppressing marker is measured before and after the individual's cells are exposed to the mutagenic agent. The individual's susceptibility to cancer as a result of the mutagenic agent is correlated with the degree to which the growth-suppressing marker is induced by exposure to the agent. A method is also disclosed for assessing cancer prophylaxis effects of compounds, such as vitamins or food extracts, in individuals. Cells from an individual are incubated with at least one compound in vitro, or the compound is directly administered to the individual, after which some of the incubated cells, as well as non-incubated cells, are exposed to a mutagenic agent such as ionizing radiation. The level of the growth-suppressing marker in the cells incubated with the compound and exposed to the mutagenic agent is then compared with the level in the non-incubated cells exposed to the agent. The cancer prophylaxis effects of the compound are correlated with a higher level of the marker in the incubated cells. | 10-23-2008 |
| 20080261208 | 2'-Nitrobenzyl-Modified Ribonucleotides - This disclosure provides novel reversibly terminated ribonucleotides which can be used as a reagent for DNA sequencing reactions. Methods of sequencing nucleic acids using the disclosed nucleotides are also provided. | 10-23-2008 |
| 20080261209 | Electrophoretic Separation Method for Analyzing Gene Expression - The following invention relates to an improved method of quantitative or qualitative analysis of gene expression of a biological material. | 10-23-2008 |
| 20080261210 | Assay Method - The invention provides a cassette-based automated assay for homocysteine. | 10-23-2008 |
| 20080261211 | Variants in complement regulatory genes predict age-related macular degeneration - Methods for identifying a subject at risk for developing AMD are disclosed, as are kits which can be used to practice the methods. The methods include identifying specific protective or risk polymorphisms or genotypes from the subject's genetic material, including polymorphisms in the BF, C2 and/or CFH genes. Microarrays and kits for use in these methods are also provided. | 10-23-2008 |
| 20080261212 | Method of Diagnosis/Prognosis of Human Chronic Lymphocytic Leukemia Comprising the Profiling of Lpl/Adam Genes - The present invention provides methods of diagnosis and prognosis of human chronic lymphocytic leukemia (CLL) in a patient in need thereof and methods to determine IgVH mutational status. The methods of the present invention involve measuring the expression profile of two known genes: LPL and ADAM29; and comparing the ratio of their expression to diagnose the presence of CLL or to prognose the likelihood of developing CLL or the symptoms consistent with CLL. | 10-23-2008 |
| 20080261213 | Assay - The invention relates to a method for screening for pre-eclampsia in a mammal, such as a human, by determining the amount and quality of foetal 2,3 bisphosphoglycerate mutase (2,3 BPGM) present. Reagents and kits for carrying out the method are also provided. | 10-23-2008 |
| 20080261214 | DNA polymorphisms in sterol-regulator-element binding proteins - The invention relates to DNA polymorphisms in sterol regulator element binding proteins (SREBP) that are characteristic of a higher risk of genetic diseases in humans such as hyperchlolesterolemia. The corresponding polymorphisms, especially the polymorphisms on SREBP-1 and SREBP-2 are frequently observed in Alzheimer patients (SREBP-2). They are also characterized by a specific behavior in the therapy of HIV patients with proteas inhibitors and appear to have an influence on the mortality. | 10-23-2008 |
| 20080261215 | Method for Evaluating the Allergen Sensitivity of an Individual - The present invention discloses a method for evaluating the allergen sensitivity of an individual and/or the clinical efficacy of an allergen immunotherapy comprising the steps: providing at least two samples selected from the group consisting of blood or fractions thereof, connective tissue, nasal, bronchial, skin or gut biopsy material from an individual subjected or intended to be subjected to an immunotherapy with at least one pure allergen or derivative thereof, wherein the samples contain cells capable of releasing mediators in response to said allergen, contacting said sample with said allergen or derivative thereof, and determining the amounts of mediators released from said sample and evaluating the allergen sensitivity of the individual prior to therapy and/or the clinical efficacy of the immunotherapy by comparing said amounts. | 10-23-2008 |
| 20080261216 | HERV Group II Viruses In Lymphoma And Cancer - The present invention relates to compositions and methods for cancer diagnosis and therapy, including but not limited to, cancer markers. In particular, the present invention relates to HERV-K(HML-2) target titers as diagnostic markers, and HERV-K(HML-2) therapeutic targets for HIV-related cancers, and other cancers. | 10-23-2008 |
| 20080261217 | Methylation Profile of Cancer - The present invention relates to compositions and methods for cancer diagnostics, including but not limited to, cancer markers. In particular, the present invention provides methods of identifying methylation patterns in genes associated with specific cancers. | 10-23-2008 |
| 20080261218 | Detection of Biomarkers for Neuropsychiatric Disorders - Systems and methods provide a comprehensive high-throughput approach toward the sequential identification, prioritization, verification, and validation of etiologic factors in neuropsychiatric disorders, some of which can also be utilized as biomarkers for these illnesses. The systems and methods determine patterns of gene expression in various tissues from various samples under various experimental and non-experimental conditions, and uses the differences and similarities between the gene expression profiles observed under these conditions to delineate distinct gene expression profiles of risk and treatment of neuropsychiatric disorders. | 10-23-2008 |
| 20080261219 | Polynucleotide Primers - A polynucleotide primer comprising at least the final six nucleotides of one of the following primer sequences, or a sequence complementary thereto: SEQ. ID NOS. 1 to 18, 21 to 45 or 74 to 77. | 10-23-2008 |
| 20080261220 | Nucleic Acid Detection Assays - The present invention relates to novel methods of producing oligonucleotides. In particular, the present invention provides an efficient, safe, and automated process for the production of large quantities of oligonucleotides. | 10-23-2008 |
| 20080261221 | AUTOMATED VNTR GENOTYPING METHOD - The invention provides an automated VNTR genotyping method using multiple variable-number tandem repeats (VNTRs) loci based on mycobacterial interspersed repetitive units (MIRU) undergoing multiplex PGR and high throughput MEGABACE® capillary electrophoresis system. The method uses fluorescent dyes of 6-carboxytetramethylrhodamine(TAMRA), 6-carboxy fluorescein (FAM) and 6-carboxy-2′, 4, 4′, 5′, 7, 7′-hexachlorofluorescein (HEX) labeling PGR primers. The method results in an efficient VNTR genotyping with low cost, less labor-requirement and less reaction time. The method is applicable in organism analyses by VNTR genotyping, such as microorganisms, parasites, animals or plants. | 10-23-2008 |
| 20080261222 | Rapid and comprehensive identification of prokaryotic organisms - An improved method for rapid identification of microorganisms is disclosed, along with sequences of PCR primers optimized for this purpose. The primers are designed based on information analysis of sequences from a large number of organism to amplify certain segments of genomic DNA whose sequences are unique among different organisms. The PCR products are compared with a DNA sequence database to obtain the identity of the microorganisms. This approach provides an accurate and fast identification and taxonomic assignment of microbial species. | 10-23-2008 |
| 20080261223 | ARL-1 Specific Antibodies - This invention provides antibodies immunologically specific for human ARL-1 (also referred to AKR1B10), a species of the aldo-keto reductase superfamily of proteins. The invention also provides methods of making and methods of using said antibodies. | 10-23-2008 |
| 20080261224 | PGC-1Beta, A Novel PGC-1 Homologue and Uses Therefor - The invention provides isolated nucleic acid molecules, designated PGC-1β nucleic acid molecules, which encode novel PGC-1 related coactivator molecules. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing PGC-1β nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a PGC-1β gene has been introduced or disrupted. The invention still further provides isolated PGC-1β proteins, fusion proteins, antigenic peptides and anti-PGC-1β antibodies. Diagnostic and therapeutic methods utilizing compositions of the invention are also provided. | 10-23-2008 |
| 20080261225 | METHOD AND KIT FOR DETECTING A TARGET PROTEIN USING A DNA APTAMER - A method and a kit for detecting a target protein in a sample with a signal amplification strategy are provided. The signal amplification strategy is established for the aptamer-based molecular recognition of a target protein with concomitant release of single-stranded DNA (G-DNA), which binds complementarily to a single-stranded RNA comprising a fluorophore and a quencher (“F-RNA-Q”). The fluorescence-quenched RNA is then degraded by RNase H to result in a fluorescence signal, and the undamaged G-DNA is recycled to yield fluorescence amplification. | 10-23-2008 |
| 20080261226 | Biomarkers of neurodegenerative disease - The present invention provides biomarkers and diagnostic methods employing such biomarkers based on the discovery of genes that have a two-fold or greater difference in gene expression in the spinal cord of a pre-symptomatic mouse model of amyotrophic lateral sclerosis. Such biomarkers and diagnostic methods are useful for early detection of neural cell injury and death in acute and degenerative disease. | 10-23-2008 |
| 20080261227 | Kinetic Pcr Assay for Quantification of Gene Amplification on Chromosome 17 - Provided is a kinetic PCR (“kPCR”) assay for determining gene copy number of a target gene located on chromosome 17. The kPCR assay uses the MMP-28 gene located at the 17q11.2-17q12 loci as a control and thus, is capable of detecting gene copy number of any gene on chromosome 17 in both singleplex and multiplex format without the need for a standard curve. The kPCR assay is useful for determining the gene copy number of the HER2/neu gene located at loci 17q12-17q21.32, which is a requirement for determining if a breast cancer patient is a candidate for anti-HER2/neu gene therapy. | 10-23-2008 |
| 20080261228 | PCA3 Messenger RNA Species in Benign and Malignant Prostate Tissues - This invention concerns the discovery of two distinct PCA3 mRNA sequences. One of these sequences corresponds to a short PCA3 mRNA molecule whereas the other PCA3 RNA molecule is longer as it comprises an additional sequence between exon 3 and exon 4a. The short RNA is associated with prostate cancer whereas the long RNA sequence is associated with a non-malignant state of the prostate. Based on the differential expression levels of these two PCA3 RNA sequences, protocols for the diagnosis of prostate disease are provided. The invention also relates to therapeutic approaches to prostate cancer. | 10-23-2008 |
| 20080261229 | SIMULTANEOUS RAPID DETECTION OF MICROBES - The invention includes a method of simultaneously detecting the presence or absence of more than one microbe class (e.g., bacteria, yeast, and mold) in a sample. The method can include the step of applying a sample suspected of containing more than one microbe class to a growth medium, optionally fragmenting the sample, associating the sample with a labeling agent, and simultaneously detecting the presence or absence of each microbe class by detection of the labeling agent. | 10-23-2008 |
| 20080261230 | FERMENTIVE PRODUCTION OF ISOBUTANOL USING HIGHLY ACTIVE KETOL-ACID REDUCTOISOMERASE ENZYMES - Methods for the fermentative production of isobutanol is provided by the fermentative growth of a recombinant microorganism expressing a highly active ketol-acid reductoisomerase enzyme in addition to other enzymes required for conversion of glucose to isobutanol. | 10-23-2008 |
| 20080261231 | DIABETES GENE - The present invention relates generally to the field of human genetics. Specifically, the present invention relates to methods and materials used to isolate and detect human diabetes mellitus predisposing gene, specifically the angiotensinogen (AGT) gene, some mutant alleles of which cause susceptibility to insulin-dependent diabetes mellitus (IDDM). More specifically, the invention relates to germline mutations in the AGT gene and their use in the diagnosis of predisposition to diabetes. The invention also relates to the prophylaxis and/or therapy of diabetes associated with a mutation in the AGT gene. The invention further relates to the screening of drugs for diabetes therapy. Finally, the invention relates to the screening of the AGT gene for mutations, which are useful for diagnosing the predisposition to diabetes. | 10-23-2008 |
| 20080268428 | Chromosome 5 Genetic Variants Related to Dyslexia - An isolated polynucleotide or genetic material from human Chromosome 5 that indicates the presence of dyslexia or a predisposition to develop dyslexia in the individual from whom which the sample was obtained. A method of diagnosing dyslexia or a predisposition to develop dyslexia. | 10-30-2008 |
| 20080268429 | Rna - Containing Microvesicles and Methods Therefor - Contemplated compositions and methods are directed to the use of microvesicles from an optionally recombinant donor cell to impart a desirable effect to a recipient cell. In certain preferred aspects, RNA of the microvesicles is employed to achieve the desirable effect. For example, microvesicles are used in vitro to increase the number of passages of a cell growing in a medium, reduce serum and/or growth factor requirements of a cell growing in a medium, and/or delay differentiation of a cell growing in a medium. Further preferred aspects include use of the microvesicles as therapeutic agents in which RNA, a membrane protein, and/or a cytosolic protein encapsulated in or coupled to the microvesicle provide a therapeutic effect. Additionally, diagnostic methods are contemplated in which RNA of a microvesicle isolated from a mammal is associated with a condition of the mammal. | 10-30-2008 |
| 20080268430 | Methods and Kits Pertaining to the Detection, Identification and Quantification of Bacteria and Yeast in Wine, Beer and Juices - The invention concerns new analytical methods for detecting and identifying qualitatively and quantitatively germ contaminations, e.g. bacterial and yeast contaminations usings specific DNA sequences, indicating living and dead germs within 12 hours time or less. The DNA is amplified and as quantified. The invention also embraces kits. | 10-30-2008 |
| 20080268431 | Information Code System Using Dna Sequences - The present invention provides a molecular level of DNA information code which uses a base pair sequence as an information code unit. Also, the present invention provides a molecular code system which includes designing and coding DNA which is an information code unit; stabilizing the DNA information code by encapsulating it with an inorganic capsule and coating the DNA-inorganic capsule to a medium; taking and extracting the coated DNA information code which is present in a trace amount, collecting the DNA information code using a polypyrrole-maghemite nanohybrid; and amplifying the collected DNA information code using a polymerase chain reaction and reading the amplified DNA information code. According to the present invention, the DNA information code having high security is prepared by assigning a security unit to a DNA which has an excellent accumulating capacity, and then the DNA information code is stabilized so as to be coated to a medium. Only the DNA information code may be extracted, collected, and read, if necessary. Thus, a unified molecular code system can be established. | 10-30-2008 |
| 20080268432 | Method for detection of staphylococcus epidermidis - A method for detecting the bacteria | 10-30-2008 |
| 20080268433 | Use of Mitochondrial Point Mutations as Sensitive Clonal Markers - The present invention relates to a method of detecting an aberrant population of cells in a subject and, more particularly, to a method of qualitatively and/or quantitatively detecting a clonal population of aberrant cells in a subject by ‘screening for mitochondrial DNA mutations. The method of the present invention is useful in a range of applications including, but not limited to, diagnosing a condition characterised by the presence of a clonal population of aberrant cells (such as a neoplastic condition), monitoring the progression of such a condition, predicting the likelihood of a subject's relapse from a remissive state to a disease state or for assessing the effectiveness of existing therapeutic drugs and/or new therapeutic agents. In a related aspect, the present invention also provides a method of characterising clonal populations of aberrant cells by determining the nature and range of mitochondrial DNA mutations expressed by a specific population of aberrant cells. | 10-30-2008 |
| 20080268434 | Temperature Control of Reaction Vessel, System with Reaction Vessel, Software Product for System and Use of System - The invention relates to a method for rapid thermal control of a reaction volume ( | 10-30-2008 |
| 20080268435 | Brca1 Markers - A method of predicting the presence of a non-functional BRCA1 gene in a biological sample, comprises the step of assaying the sample for expression of at least one specific member of the S100 family of genes. The invention also describes a kit for predicting the presence of non-functional BRCA1, comprising means for assaying a sample for expression of at least one specific member of the S100 gene family. A method of detecting a genetic predisposition to cancer is also described, comprising the step of assaying a biological sample for expression of a specific member of the S100 family of genes. Also described is a method of determining a suitable chemotherapeutic agent for an individual. | 10-30-2008 |
| 20080268436 | Schizophrenia, Schizoaffective Disorder and Bipolar Disorder Susceptibility Gene Mutation and Applications to Their Diagnosis and Treatment - The present invention provides the identification of a number of SNPs that are associated schizophrenia, schizoaffective disorder, bipolar disorder and related mental disorders which were found to be strongly linked to individuals with the disease. The invention provides SNP locations on human chromosome 6, as well as methods of making PCR primers and assays for detecting the SNPs in tested individuals. | 10-30-2008 |
| 20080268437 | Method of Targeted and Comprehensive Sequencing Using High-Density Oligonucleotide Array - A method for targeted and comprehensive sequencing using high-density oligonucleotide array, comprising the steps of hybridizing nucleic acid from an investigative species with high-density oligonucleotide arrays of a related species, identifying the oligonucleotide probes that generate high hybridization signals, using the probes sequences to make PCR primers, amplifying heterologous genes by PCR with the gene specific PCR primers and an anchoring primer, and sequencing the PCR products. | 10-30-2008 |
| 20080268438 | MODEL SYSTEM FOR IDENTIFYING ANTI-CANCER AGENTS - A model system for screening and identification of compounds that interfere with Gli2 dependent tumorigenesis and provide potential use as anticancer agents is provided. In particular, the invention includes a Gli2 protein having an S662A point mutation that interferes with binding by the ubiquitin-ligase β-TrCP. The mutation inhibits Gli2 degradation by the ubiquitin pathway. Gli2 stability and half-life are increased in the host cell resulting in an increase in Gli2-dependent transcription and concomitant neoplasia and tumorigenesis. Expression of the Gli2 mutant allows for the high throughput screening of compounds that interfere with the tumorigenesis thereby identifying anticancer agents. | 10-30-2008 |
| 20080268439 | ASSAY SYSTEMS AND METHODS FOR DETECTING MOLECULES THAT INTERACT WITH SK2 CHANNELS - The invention provides methods including steps of: providing cells capable of expressing SK2; contacting the cells with a test molecule; obtaining information indicative of cellular SK2 expression to obtain an SK2 Expression Value; comparing the SK2 Expression Value with a control SK2 Expression Value; and identifying a test molecule that causes the cells to display an SK2 Expression Value that is different from the control SK2 Expression Value. Also provided are methods including steps of: providing a sample comprising an SK2 channel; contacting the sample with a test molecule; obtaining information indicative of SK2 channel activity in the sample to obtain an SK2 Channel Activity Value; comparing the SK2 Channel Activity Value with a control Channel Activity Value; and identifying a test molecule that causes the SK2 Channel Activity Value to be different from the control Channel Activity Value. Methods of identifying a molecule useful for treating neuropathic pain are also described. | 10-30-2008 |
| 20080268440 | BIOMOLECULE IMMOBILIZATION ON SURFACE VIA HYDROPHOBIC INTERACTIONS - A method, apparatus, or system for generating a pattern of polynucleotides on a substrate. The method includes providing a substrate having a hydrophobic surface. The method further includes conjugating a polystyrene moiety to a polynucleotide and applying a polystyrene-polynucleotide conjugate to create a plurality of reaction spots on the hydrophobic surface of the substrate. An apparatus includes a substrate with at least one polystyrene-polynucleotide conjugate on a surface of the substrate. A system can analyze a polystyrene-polynucleotide conjugate and the system may perform PCR. | 10-30-2008 |
| 20080268441 | DETECTABLE LABELED NUCLEOSIDE ANALOGS AND METHODS OF USE THEREOF - The invention relates to detectable labels useful for detection of nucleotide sequences. Specifically, the invention relates to labeled-imidazole-PEG compounds, such as nucleosides, nucleotides, and nucleic acids incorporating such compounds, and methods utilizing such compounds. The invention further relates to kits comprising labeled imidazole-PEG compounds. | 10-30-2008 |
| 20080268442 | METHOD AND SYSTEM FOR PREPARING A BLOOD SAMPLE FOR A DISEASE ASSOCIATION GENE TRANSCRIPT TEST - System and method for preparing a blood sample for a disease association gene transcript test. Disease considerations for this unique test include a custom set of genetic sequences associated in peer-reviewed literature with various known diseases such as Addison's disease, anemia, asthma, atherosclerosis, autism, breast cancer, estrogen metabolism, Grave's disease, hormone replacement therapy, major histocompatibility complex (MHC) genes, longevity, lupus, multiple sclerosis, obesity, osteoarthritis, prostate cancer, and type 2 diabetes. The base dataset may be developed through clinical samples obtained by third-parties. Online access of real-time phenotype/genotype associative testing for physicians and patients may be promoted through a testing service. | 10-30-2008 |
| 20080268443 | BROAD-BASED DISEASE ASSOCIATION FROM A GENE TRANSCRIPT TEST - Broad-based disease association gene transcript test and data structure. Disease considerations for this unique test include a custom set of genetic sequences associated in peer-reviewed literature with various known diseases such as Addison's disease, anemia, asthma, atherosclerosis, autism, breast cancer, estrogen metabolism, Grave's disease, hormone replacement therapy, major histocompatibility complex (MHC) genes, longevity, lupus, multiple sclerosis, obesity, osteoarthritis, prostate cancer, and type 2 diabetes. The base dataset may be developed through clinical samples obtained by third-parties. Online access of real-time phenotype/genotype associative testing for physicians and patients may be promoted through an analysis of a customized microarray testing service. | 10-30-2008 |
| 20080268444 | Detection method of SNPs - A method for detecting a mismatch between a target nucleic acid as a measuring object and a control nucleic acid, the method comprising: (a) effecting formation of a double-stranded nucleic acid through hybridization of the control nucleic acid and the target nucleic acid; (b) allowing a mismatch binding protein to contact with the double-stranded nucleic acid and thereby to bind to a mismatched site; (c) allowing an intercalating agent which specifically recognizes the double-stranded nucleic acid and is intercalated therein, to contact with the double-stranded nucleic acid; (d) detecting the intercalating agent intercalated into the double-stranded nucleic acid; and (e) judging the presence or absence of a mismatch between the control nucleic acid and the target nucleic acid, by comparing amounts of the intercalating agent intercalated into the double-stranded nucleic acid in the absence and presence of the mismatch binding protein. | 10-30-2008 |
| 20080268445 | Ant2 Conditional Knockout Mouse and Methods - Described are methods for inactivating adenine nucleotide transporter proteins in specific tissues of a transgenic nonhuman animal using a conditional knockin/knockout technology such as the Cre-LoxP, Flip-FLP recombinase, or Tet-on/off technologies. Specifically, the Ant2 gene is functionally inactivated in a mouse in liver, with or without the concurrent inactivation of the Ant1 gene. The result is an animal in which the Ant2 gene and accompanying ANT 2 protein is absent in one or more tissues, either in the presence or absence of the Ant1 gene and accompanying protein. The resulting animals, cells, mitochondria, and subcelluar fractions such as the mitochondrial permeability transition pore can then be used to identify agents that affect animal and/or subcellular function via a direct or indirect interaction with the ANT2 protein and/or its Ant2 gene. | 10-30-2008 |
| 20080268446 | Flexible Culture Medium Bag Containing Nutrient Concentrate - Disclosed is a culture medium container such as a bag comprising a main compartment and a locus of containment that contains a nutrient concentrate until it is released at the time of use. The locus of containment may comprise separated compartments defined by frangible seals or seals comprising a water-reactive material. Sachets comprising frangible seals or a water-reactive material are also suitable for the locus of containment. Matrices and coatings comprising water-reactive material are also suitable. Capsules that can be pulverized and/or dissolved may also be used. | 10-30-2008 |
| 20080268447 | Sequential Cloning System - This invention discloses a cloning system and more particularly a system for sequentially cloning a plurality of heterologous nucleic acid sequences to assemble a chimeric construct of interest. The cloning system employs a marker sequence, which confers an identifiable characteristic on host cells in which it is contained, to chaperone individual insert nucleic acid sequences into recipient constructs that do not comprise the marker sequence but comprise other nucleic acid sequences for inclusion in the chimeric construct. Recombinant constructs into which one or more insert nucleic acid sequences have been introduced with the chaperone marker sequence are isolated by introducing recombinant constructs into host cells and identifying hosts cells with the identifiable characteristic. | 10-30-2008 |
| 20080268448 | Multiplex compositions and methods for quantification of human nuclear DNA and human male DNA and detection of PCR inhibitors - The invention relates to a method for simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction. The multiplex quantification method also provides a ratio of human nuclear and male DNA present in a biological sample. Such sample characterization is useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping. | 10-30-2008 |
| 20080268449 | UTILITY OF B-RAF DNA MUTATION IN DIAGNOSIS AND TREATMENT OF CANCER - The present invention discloses a method of detecting a wild-type or mutant B-RAF gene in a body fluid sample from a subject. Also disclosed are methods of using B-RAF as a biomarker for detecting cancer, predicting the outcome of cancer, and monitoring the treatment of cancer or the status of cancer. Furthermore, the invention discloses methods and compositions for detecting a mutant gene with a peptide nucleic acid clamp capable of hybridizing to a wild-type gene and a locked nucleic acid probe capable of hybridizing to a mutant of the gene. | 10-30-2008 |
| 20080268450 | AMPLIFICATION ASSAY FOR ANALYTE DETECTION - The present invention provides a method for detecting an analyte of interest via a bio-barcode assay. The present invention provides a calorimetric bio-barcode method that is capable of detecting minute concentrations of an analyte by relying on porous particles, which enable loading of a large number of barcode DNA per particle, and a metal particle-based colorimetric barcode detection method. | 10-30-2008 |
| 20080268451 | MEASUREMENT OF AN INSOLUBLE ANALYTE IN A SAMPLE - The present invention relates to compositions, apparatus and methods useful for concurrently performing singular, multiple, high throughput, biological or chemical assays, using nuclease protection molecules which specifically bind to a target of interest. The nuclease protection molecules are capable of detecting targets in complex biological samples, including, preserved, fixed, dried, and/or cross-linked specimen. The reagents and methods of the instant invention provide an effective means for analyzing a target of interest from a complex biological sample without solubilizing or disrupting the sample. Utilization of such methods in clinical and/or diagnostic applications is also described. | 10-30-2008 |
| 20080268452 | COMPOSITIONS, KITS AND RELATED METHODS FOR THE DETECTION AND/OR MONITORING OF PSEUDOMONAS AERUGINOSA - The present invention provides compositions, methods and kits for the species-specific detection of | 10-30-2008 |
| 20080268453 | Types of lymphoma and method for prognosis thereof - A method for determining the prognosis of a CD5+DLBCL patient and a CD5-DLBCL patient is provided. It is determined that, in the chromosomal DNA from a patient with lymphoma, (1) the prognosis of the CD5+DLBCL patient with amplification of 13 | 10-30-2008 |
| 20080268454 | Compositions, methods and systems for inferring bovine breed or trait - Methods, compositions, and systems are provided for managing bovine subjects in order to maximize their individual potential performance and edible meat value, and to maximize profits obtained in marketing the bovine subjects. The methods and systems draw an inference of a trait of a bovine subject by determining the nucleotide occurrence of at least one bovine SNP that is identified herein as being associated with the trait. The inference is used in methods of the present invention to establish the economic value of a bovine subject, to improve profits related to selling beef from a bovine subject; to manage bovine subjects, to sort bovine subjects; to improve the genetics of a bovine population by selecting and breeding of bovine subjects, to clone a bovine subject with a specific trait, to track meat or another commercial product of a bovine subject; and to diagnose a health condition of a bovine subject. Methods are also disclosed for identifying additional SNPs associated with a trait, by using the associated SNPs identified herein. | 10-30-2008 |
| 20080268455 | DETECTION OF NUCEIC ACIDS BY MULTIPLE SEQUENTIAL INVASIVE CLEAVAGES - The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample. | 10-30-2008 |
| 20080268456 | METHOD FOR DETECTING AND QUANTITATING MULTIPLE-SUBCELLULAR COMPONENTS - A method for detecting and quantitating multiple and unique fluorescent signals from a cell sample is provided. The method combines immunohistochemistry and a fluorescent-labeled in situ hybridization techniques. The method is useful for identifying specific subcellular components of cells such as chromosomes and proteins. | 10-30-2008 |
| 20080268457 | siRNA targeting forkhead box P3 (FOXP3) - Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed including those directed to nucleotide sequences for FOXP3. | 10-30-2008 |
| 20080268458 | Method of Preparing Nucleic Acids for Detection - A method is provided for preparing a test sample for detecting a predetermined target nucleic acid. The method includes the steps of providing a test probe comprising an oligonucleotide attached to a nanoparticle and providing a hybridization unit containing the test sample and the test probe, wherein said hybridization unit further includes a target sample substrate and a distribution manifold coupled to a first side of the substrate. The method further includes the steps of clamping a processing fluids manifold to the distribution manifold of the hybridization unit, denaturing the test sample and preparing the test sample for detecting the predetermined target nucleic acid by pumping a plurality of processing fluids between the processing fluids source manifold and distribution manifold to hybridize the test probe and predetermined target nucleic acid to the target sample substrate, to wash the hybridized sample and to amplify a detectable parameter of the hybridized sample. | 10-30-2008 |
| 20080274452 | Process For Large Scale Production Of Plasmid Dna By E.Coli Fermentation - The present invention relates generally to a method for increasing the yield of plasmid DNA production. The method includes the steps of selecting a highly productive clonal subtype of a strain of | 11-06-2008 |
| 20080274453 | Diagnostics and therapeutics for macular degeneration - The invention relates to diagnostics and therapeutics and animal models for macular degeneration, specifically as they relate to the association described herein between macular degeneration and arterial wall disruptive disorders. In one embodiment, the invention provides kits and methods for diagnosing macular degeneration comprising identifying a marker for an arterial wall disruptive disorder, including an aneurysm. In one embodiment, the invention provides therapeutics for treating macular degeneration comprising delivering to a subject an agent useful for treating an arterial wall disruptive disorder, including an aneurysm. | 11-06-2008 |
| 20080274454 | Reversible and Chemically Programmable Micelle Assembly With Dna Block-Copolymer Amphiphiles - The present invention is directed to amphiphilic block copolymers. More particularly the present invention is directed to amphiphilic block copolymers comprising a polynucleotide block and a hydrophobic polymer block, to micelles formed from the block copolymers, and to methods of using the micelles. | 11-06-2008 |
| 20080274455 | Use Of Genes As Molecular Markers In Diagnosis Of Schizophrenia And Diagnostic Kit For The Same - Drug-naive and drug-free schizophrenic PBL were screened to identify additional markers that are differentially expressed compared to healthy individuals using microarray and quantitative real-time PCR (QRT-PCR) techniques. Genes for dopamine D | 11-06-2008 |
| 20080274456 | Methods and Compositions for Modifying Gene Regulation and Dna Damage in Ageing - The invention relates to gene regulation in ageing, and age-related cognitive decline. The invention, in particular relates to methods for screening a subject for a propensity to develop diseases associated with oxidative stress, and for age-related conditions, by examining the up-regulation and/or down-regulation of at least one gene associated within the central nervous system. | 11-06-2008 |
| 20080274457 | METHODS FOR DIFFERENTIATING MALIGNANT FROM BENIGN THYROID TISSUE - Methods of identifying malignant thyroid tissue comprising testing a thyroid tissue sample for the expression of at least two genes chosen from CCND2, PCSK2, and PLAB. Kits for use in the disclosed methods are also provided. | 11-06-2008 |
| 20080274458 | NUCLEIC ACID QUANTITATION METHODS - The invention relates to a method of determining the amount of a target nucleic acid sequence in a sample, the method comprising: obtaining multiple distinguishable amplicons of the target nucleic acid sequence, each comprising a distinguishing tag and a target portion; amplifying the amplicons in a single reaction volume; and detecting nucleic acids amplified from the amplicons. Detection of the distinguishable amplicons can be varied in each of the steps of the method, which expands the dynamic range of the nucleic acid quantification methods and improves the reliability and accuracy of the methods. | 11-06-2008 |
| 20080274459 | METHOD FOR COLLECTING AND STORING BIOLOGICAL MATERIALS - A method for collecting and storing biological material includes the steps of obtaining a patient identity of a patient and a biological material from a patient. A DNA identity analysis is performed to establish a DNA identity for the patient. The DNA identity is associated with the patient identity and the biological material. The biological material is stored in a preserving environment, and the patient identity and the DNA identity are stored in a database. A request for the biological material is received from a requestor purporting to have the patient identity. A DNA sample from said requestor is obtained. A DNA identity analysis is performed on the DNA sample from the requestor to obtain a DNA identity of the requestor. The DNA identity of the requestor is then compared to the stored DNA identity of the patient, and if the DNA identities match, the biological material is provided to the requestor. | 11-06-2008 |
| 20080274460 | Common allele on chromosome 9 associated with coronary heart disease - Disclosed are methods and compositions for determining whether a person carries an allele associated with increased risk for coronary atherosclerosis by determining whether the person has had RA-CHR9 allel, such as by determining whether the person has an RA-CHR9 allele-associated single nucleotide polymorphism (SNP). | 11-06-2008 |
| 20080274461 | Perinucleolar Compartment Markers for Cancer - The present invention relates to compositions and methods for cancer diagnostics, prognostics and predictions, including but not limited to, cancer markers. In particular, the present invention provides perinucleolar compartments and their resident molecules as cancer markers. | 11-06-2008 |
| 20080274462 | Universal bases for nucleic acid analyses, methods for using universal bases, and kits comprising universal bases - Compounds, methods and kits for making and analyzing primer extension products incorporating one or more universal bases are described, including methods and kits for nucleic acid sequencing and microsatellite analysis. | 11-06-2008 |
| 20080274463 | Method for quantifying biomolecules conjugated to a nanoparticle - Disclosed embodiments concern quantifying a biomolecule conjugated to a nanoparticle. Quantifying typically comprises determining the number of biomolecules per nanoparticle. Any suitable biomolecule can be used, including but not limited to, amino acids, peptides, proteins, haptens, nucleic acids, oligonucleotides, DNA, RNA, and combinations thereof. A single type of biomolecule may be conjugated to the nanoparticle, more than one biomolecule of a particular class may be conjugated to the nanoparticle, or two or more classes of biomolecules may be conjugated to the nanoparticle. Certain disclosed embodiments comprise enzymatically or chemically digesting a biomolecule conjugated to the nanoparticle, or displacing a biomolecule using ligand-exchange chemistry. Where biomolecule concentrations are determined, any technique suitable for determining biomolecule concentration can be used, such as spectrophotometric techniques, including measuring tryptophan fluorescence and using a standard fluorescence intensity versus biomolecule concentration curve. | 11-06-2008 |
| 20080274464 | Gene Detecting Method - Disclosed is a gene detecting method for determining mutation of a specific base or presence/absence of a specific base in a target gene. There are provided a the target gene sample and a control gene sample having a base sequence which is wild-type or standard-type with respect to the target gene. The method comprises steps of (i) independently subjecting the target gene sample and the control gene sample to a PCR reaction for amplification, using primers having an RNA polymerase promoter sequence at the 5′-end thereof, (ii) independently subjecting the double-stranded DNAs produced by said PCR reaction from the target gene sample and from the control gene sample, to an in vitro transcription reaction to form a single-stranded RNA, (iii) independently hybridizing the single-stranded RNAs with a fluorescence-labeled probe composed of a single-stranded DNA having a base sequence complementary to at least part of the base sequence of the control gene and being combined with a fluorescent dye, to form an RNA/DNA hybrid, and then (iv) comparing the fluorescence intensity of the RNA/DNA hybrid derived from the target gene sample with that of the RNA/DNA hybrid derived from the control gene sample. | 11-06-2008 |
| 20080274465 | Method for Breast Cancer Diagnosis/Prognosis - The present invention relates to a method for breast cancer diagnosis/prognosis comprising the following steps:
| 11-06-2008 |
| 20080274466 | Enrichment Through Heteroduplexed Molecules - The present invention relates to the enrichment of specific target sequences Enrichment can be achieved through the formation of a heteroduplex that includes the specific target sequence and then the specific cleavage of the heteroduplex. A binding moiety is then added to the cleaved heteroduplex, allowing for the subsequent manipulation of the specific target sequence in the heteroduplex. | 11-06-2008 |
| 20080274467 | Novel Therapeutic Targets in Cancer - The present invention relates to novel sequences for use in detection, diagnosis and treatment of cancers, especially lymphomas. The invention provides cancer-associated (CA) polynucleotide sequences whose expression is associated with cancer. The present invention provides CA polypeptides associated with cancer that are present on the cell surface and present novel therapeutic targets against cancer. The present invention further provides diagnostic compositions and methods for the detection of cancer. The present invention provides monoclonal and polyclonal antibodies specific for the CA polypeptides. The present invention also provides diagnostic tools and therapeutic compositions and methods for screening, prevention and treatment of cancer. | 11-06-2008 |
| 20080274468 | NOVEL MEANS FOR THE DIAGNOSIS AND THERAPY OF CTCL - The invention relates to a novel molecule, termed SC5 by the inventors, to a novel allelic form of p140, and to the biological applications of SC5 and p140 molecules, notably in the diagnosis and therapy of CTCL. | 11-06-2008 |
| 20080274469 | C-Kit Oncogene Mutations in Melanoma - The present invention provides methods of detecting c-KIT-dependent-melanoma for diagnostic and prognostic purposes. The invention further provides methods of treating such melanoma by inhibiting c-KIT. | 11-06-2008 |
| 20080274470 | NON-INVASIVE DETECTION OF ENDOMETRIAL CANCER - The present invention provides a non-invasive method of obtaining a sample of endometrial cells for use in the diagnosis of endometrial cancer, as well as methods and kits for diagnosing, determining the prognosis of, and monitoring endometrial cancer. | 11-06-2008 |
| 20080274471 | Methods for detecting an increased risk for coronary heart disease - The invention relates generally to an allele on human chromosome 9 associated with increased risk for coronary heart disease and the use or detection of such an allele in determining whether a human has an increased risk for coronary heart disease. In one aspect, the invention relates to methods for detecting a predisposition or propensity or susceptibility for coronary heart disease in a human, comprising detecting the presence of an allele on human chromosome 9 that is associated with an increased risk for coronary heart disease in a human. Disclosed are methods and compositions for determining whether a person carries an allele associated with increased risk for coronary atherosclerosis by determining whether the person has an RA-CHR9 allele, such as by determining whether the person has an RA-CHR9 allele-associated single nucleotide polymorphism (SNP). The invention also relates to kits for detecting the presence of an allele on chromosome 9 associated with an increased risk for coronary heart disease. | 11-06-2008 |
| 20080274472 | Novel kinases and uses thereof - Novel kinase polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length kinase proteins, the invention further provides isolated kinase fusion proteins, antigenic peptides, and anti-kinase antibodies. The invention also provides kinase nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a kinase gene has been introduced or disrupted. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided. | 11-06-2008 |
| 20080280286 | Compositions and Methods for Gene Expression - The invention provides nucleotide sequences that mediate one or more functions of IKKα, kits and methods for using these sequences to identify therapeutic compounds that alter IKKα related pathology. | 11-13-2008 |
| 20080280287 | Oligonucleotides For the Detection of Hepatitis B Virus - The invention relates to specific oligonucleotide primers and probes for rapid and sensitive detection of hepatitis B virus by amplification of the HBV nucleic acid, and to methods using the same. It is also concerned with kits useful to perform these detection tests. | 11-13-2008 |
| 20080280288 | Assay Method to Predict Sensitivity of Cancer Cell to Anticancer Drug - If a cancer cell sampled from a cancer patient by biopsy or the like can be examined with respect to its expressed molecules to evaluate sensitivity of the cancer cell to the present compound, the present compound can be selectively administered only to the cancer patient who is expected to benefit from antitumor activity of the present compound, thereby to enhance the therapeutic effect and reduce unnecessary adverse effects. Specifically, the characteristics of a cancer cell with respect to “low level expression of pRB”, “positive expression of p16” and “high level expression of cyclin E” are demonstrated to be useful as markers for sensitivity of the cancer cell to the present compound. Therefore, these characteristics of a cancer cell can be examined to evaluate previously sensitivity of the cancer cell to the present compound. | 11-13-2008 |
| 20080280289 | Primers, Methods and Kits for Detecting Killer-Cell Immunoglobulin-Like Receptor Alleles - Embodiments of the present invention describe primer pairs, methods and kits for identifying and/or detecting killer-cell immunoglobulin-like receptor (KIR) alleles. The present primer sets include one or more primer pairs that can produce amplicons specific for an individual KIR allele and that are less than 1000 bp in size. Additionally, the primer sets can target intra-exon and/or extracellular domains of KIR alleles for amplification. | 11-13-2008 |
| 20080280290 | Method and Device for the Collection and Isolation of Nucleic Acid - A device for collecting and preserving nucleic acids in a sample, the device comprising: a) a support; b) one or more than one sample zone in the support for loading the sample onto the device; and c) a composition comprising i) one or more than one absorbent, and ii) one or more than one stabilizer; where the one or more than one sample zone on the support comprises a recess or space within the support extending from the top surface toward, but not through, the bottom surface, or comprises a space within the support and the composition is retained within the sample zone. A method for collecting and preserving nucleic acids in a sample, the method comprising a) providing a device for collecting and preserving nucleic acids in a sample according to the present invention; b) providing a sample potentially comprising one or more than one nucleic acid; and c) applying part or all of the sample to one or more than one of the sample zones on the device. A method of detecting and quantifying nucleic acids in a sample, the method comprising a) collecting and preserving nucleic acids in the sample according to a method of the present invention; b) removing the absorbent with sample from the sample zones of the device; and c) detecting, or detecting and quantifying the nucleic acids. | 11-13-2008 |
| 20080280291 | Compositions and methods utilizing DNA polymerases - The invention features a novel isolated Family B DNA polymerase, a | 11-13-2008 |
| 20080280292 | Late-PCR - A non-symmetric polymerise chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays. | 11-13-2008 |
| 20080280293 | Method of Examining Inflammatory Disease and Method of Screening Remedy for Imflammatory Disease - A gene polymorphism on a Toll-like receptor gene is analyzed and an inflammatory disease is examined based on the results of the analysis. A remedy for an inflammatory disease is screened by selecting a substance capable of altering the interaction between Toll-like receptor and galectin-2. | 11-13-2008 |
| 20080280294 | Inherited Mitochondrial Dna Mutations in Cancer - A method is provided for identifying a subject likely to have, or at risk of developing a disease condition correlated with increased reactive oxygen species (ROS), including cancer, by identifying in the subject a missense mutation in a nucleic acid of Complex III, IV and/or V of the OXPHOS system. This invention also provides a method of identifying a likelihood of having a heritable predisposition to cancer by detecting a homoplasmic missense mutation in non-tumor tissue of an OXPHOS system gene. This invention also provides a method for detecting likelihood of having cancer, predisposition to cancer, and likelihood of passing a predisposition to cancer to progeny involving identifying in non-tumor tissue of the subject a missense mutation in a complex III, IV and/or V gene of the mitochondrial OXPHOS system. The mutation may be a nuclear or mitochondrial mutation. The invention has been exemplified with respect to prostate cancer. When the mutation is homoplasmic in non-tumor tissue this is an indication it is an inherited and inheritable trait, and that the subject is likely to pass on the mutation to her progeny in the case of mutations in mitochondrial DNA or his or her progeny in the case of mutations in nuclear DNA. Both homoplasmic and heteroplasmic mutations in non-tumor tissue can indicate the presence of cancer. | 11-13-2008 |
| 20080280295 | Use of Panel of Pairs of Primers Complementary to Reporter Genes of Cell Differentiation - The present invention to a panel comprising at least two pairs of primers that are complementary to at least two different reporter genes, the expression of which are i) either up- or down-regulated upon cell differentiation, and ii) display a similar expression profile in at least two different cell lines of the same kind of cells. The cells may be blastocyst-derived stem (BS) cells or human blastocyst-derived stem (hBS) cells. Furthermore, the present invention relates to the use of a calculated expression index for quantifying and evaluating the expression of the reporter genes, which for example can be used for assessing the state of differentiation of a cell population, such as, e.g. a hBS cell population. | 11-13-2008 |
| 20080280296 | METHOD FOR DETECTION OF FOOT-AND-MOUTH DISEASE VIRUS WITH CHROMATOGRAPHIC STRIP TEST - The present invention discloses a method for detection of foot-and-mouth disease virus with chromatographic strip test. Firstly, the nucleic acid sequence of FMDV NSPs is set up, the nucleic acid sequence is amplified by the reverse transcriptase polymerase chain reaction (RT-PCR) method, the recombinant vector is constructed and performed through a prokaryotic system to transform and express the recombinant protein, and the purified recombinant protein is mass produced. Design principles of the method are based on immunoassay and chromatographic analysis. The advantages are easy and simple to handle, no need of elaborate equipment, only one drop of body fluid is required to quickly complete the qualitative test in 10-20 minutes, and operating with a portable POCT (Point of care testing) instrument to complete the quantitative detection within 40-50 minutes. | 11-13-2008 |
| 20080280297 | Compositions and Methods for Differential Diagnosis of Chronic Lymphocytic Leukemia - The invention provides compositions and methods for determining a prognosis of a B cell chronic lymphocytic leukemia (CLL) in a subject based on the level of expression of at least one marker gene. Marker genes provided by the invention are SEPTlO, KIAA0799, Hs.23133, and ADAM29. The marker genes can be used to differentially diagnose CLL in a subject based on relative gene expression levels in the subject compared to reference gene expression levels established from a clinically characterized population of patients. The invention also provides diagnostic reagents and compositions and kits based on the marker genes. | 11-13-2008 |
| 20080280298 | SATB1: A DETERMINANT OF MORPHOGENESIS AND TUMOR METASTASIS - It is proposed that cancer cells express SATB1, and that SATB1 acts as a determinant for the acquisition of metastatic activity by controlling expression of a specific set of genes that promote metastatic activity. In order for cancer cells to gain the ability to metastasize, SATB1 re-organizes or re-packages genomic sequences in a specific manner to allow a switch in the pattern of gene expression. SATB1 expression was found restricted mainly to aggressive cancer cells where it may regulate the genetic and epigenetic changes that program the steps involved in the metastatic process. The present invention describes reagents and tools to detect the SATB1 protein for use in diagnosis and prognosis of aggressive cancers and therapeutics to inhibit SATB1 protein to deplete its expression in metastatic and aggressive cancers. | 11-13-2008 |
| 20080280299 | Method for Specific Detection of Legionella Pneumophila - A method for specific detection of the presence of | 11-13-2008 |
| 20080280300 | Method - There is described a method for identifying a potential modulator of a cell signalling pathway, comprising the steps of: (a) providing a cell of a first cell type, wherein said first cell type may be differentiated to a second cell type via a progenitor cell by sequentially exposing said first cell type to two or more reaction conditions; (b) adding to or replacing at least one of said two or more reaction conditions to which the progenitor cell has been exposed with exposure to one or more different reaction conditions comprising said potential modulator; -and (c) monitoring the differentiation of the first cell type to determine formation of the second cell type. | 11-13-2008 |
| 20080280301 | Lrp4/Corin DOPAMINERGIC NEURON PROLIFERATIVE PROGENITOR CELL MARKERS - In neuron transplantation therapy, in terms of safety, it is preferable to use a cell population consisting only of a desired type of cells, and to use postmitotic neurons in consideration to avoid the risk of tumorigenesis. Moreover, greater therapeutic effects would be expected through the use of earlier progenitor cells in consideration of post-transplantation viability, proper network formation ability, and such. | 11-13-2008 |
| 20080280302 | MULTIGENE DIAGNOSTIC ASSAY FOR MALIGNANT THYROID NEOPLASM - The present invention provides methods for diagnosing, providing a prognosis, and staging thyroid cancer, using panels of molecular markers that are differentially expressed in thyroid cancer. Also provided are methods to identify compounds that are useful for the treatment or prevention of thyroid cancer. | 11-13-2008 |
| 20080280303 | G-PROTEIN COUPLED RECEPTORS HIGH-THROUGHPUT FUNCTIONAL ASSAY - Disclosed herein are methods for enabling or improving functional assays of G-protein coupled receptors through the use of co-expression of helper genes. In some cases, chimeras linking the regulatory domain of the rap1B protein to the effector region of the ras oncogene are used in conduction with existing functional assays for cellular proliferation. Furthermore, overexpression of other genes can further augment the enabling properties of ras/rap chimeras. | 11-13-2008 |
| 20080280304 | METHOD FOR DETECTING TARGET PLANT GENUS - A method for detecting species in a target plant genus comprises the steps of conducting PCR using at least one member selected from the group consisting of primers (A) and (B), which can hybridize under stringent conditions to a nucleic acid molecule having a common nucleotide sequence for all species in the target plant genus in 45S rRNA precursor gene sequence thereof, wherein 3′ end of primer (A) can complementarily bind to a base in ITS-1 sequence of the target plant genus when the primer hybridizes to the nucleic acid molecule while 3′ end of primer (B) can complementarily bind to a base in ITS-2 sequence of the target plant genus when the primer hybridizes to the nucleic acid molecule, and identifying the presence of the resulting amplification product from PCR containing at least a part of ITS-1 or ITS-2 sequence of the target plant genus | 11-13-2008 |
| 20080286760 | Means and Methods for the Determination of Camp In Vitro and In Vivo - The present invention relates to a chimeric peptide, comprising a cAMP binding moiety having only one cAMP binding site and at least two detectable labels, whereby the first of said two detectable labels is located at the carboxy terminus and the second of said two detectable labels is located at the amino terminus of said cAMP binding moiety. Said chimeric peptide of the invention is particularly useful in/for direct determination of cAMP concentrations) in vitro and/or in vivo. Furthermore, nucleic acid molecules encoding said chimeric proteins are described as well as vectors and host cells comprising the same. The present invention also provides methods for producing the chimeric protein of the invention and methods for identification and screening of molecules or compounds which are capable of modifying cAMP binding to the chimeric peptide of the invention or the biological and/or pharmacological function of adenylyl cyclases or phosphodiesterases. In addition, a method for cAMP determination in a sample and a method for the detection of cAMP in the living cell or tissue is described. Finally, a kit comprising the compounds of the present invention is disclosed. | 11-20-2008 |
| 20080286761 | Methods and Nucleic Acids for the Analysis of Gene Expression Associated with the Development of Prostate Cell Proliferative Disorders - The following application provides methods and nucleic acids for the detection of and/or differentiation between prostate cell proliferative disorders. This is achieved by the analysis of the expression status of a panel of genes, or subsets thereof. | 11-20-2008 |
| 20080286762 | Gene Detection Field-Effect Device And Method Of Analyzing Gene Polymorphism Therewith - A gene detection field-effect device provided with an insulation film ( | 11-20-2008 |
| 20080286763 | Method For The Identification Of Sepsis - The invention relates to a method for the in vitro discrimination between systemic inflammatory non-infectious conditions and systemic inflammatory infectious conditions. The method comprises the following steps: a) sample RNA is isolated from a biological sample; b) the sample RNA and/or at least one DNA which represents a gene activity that is specific for distinguishing between SIRS and sepsis and/or a specific gene or gene fragment, is marked with a detectable marker; c) the sample RNA is brought in contact with the DNA in hybridization conditions; d) control RNA is brought in contact with at least one DNA in hybridization conditions, the DNA representing a gene or gene fragment that is specific for distinguishing between SIRS and sepsis; e) the marking signals of the hybridized sample RNA and control RNA are quantitatively recorded; and f) the quantitative data of the marking signals is compared in order to make a statement as to whether genes or gene fragments that are specific for distinguishing between SIRS and sepsis are expressed more prominently or less prominently in the sample than in the control RNA. | 11-20-2008 |
| 20080286765 | Human Obesity Susceptibility Gene Encoding a Taste Receptor and Uses Thereof - The present invention discloses the identification of a human obesity susceptibility gene, which can be used for the diagnosis, prevention and treatment of obesity and related disorders, as well as for the screening of therapeutically active drugs. The invention more specifically discloses that the TAS1R1 gene on chromosome 1 and certain alleles thereof are related to susceptibility to obesity and represent novel targets for therapeutic intervention. The present invention relates to particular mutations in the TAS1R1 gene and expression products, as well as to diagnostic tools and kits based on these mutations. The invention can be used in the diagnosis of predisposition to, detection, prevention and/or treatment of coronary heart disease and metabolic disorders, including hypoalphalipoproteinemia, familial combined hyperlipidemia, insulin resistant syndrome X or multiple metabolic disorder, coronary artery disease, diabetes and dyslipidemia. | 11-20-2008 |
| 20080286766 | Comparing Method for Expression Amount of the Same Gene from Different Sources by Base Sequence Measurement - The present invention relates to an analysis method used to quantitatively compare the expression levels of the same gene from different sources. The method of the present invention can be used to quantitatively compare the gene expression level difference of the same gene of tissues or cells from different sources, making use of the quantitative characteristics of bioluminescent assay and the principle of adding different deoxyribonucleic acids (dNTP) one by one. The concrete steps are: reverse transcript the messenger ribonucleic acids (mRNA) from different sources into cDNA, and label a segment of source specific sequence in cDNA from each source; mix the labeled cDNA of different sources into one tube and use it as the substrate of polymerase chain reaction (PCR); PCR amplification is performed using the same common primer and a gene-specific primer; Detect the base sequence by bioluminescent assay, wherein the base type represents the different gene source, and the signal intensity of each base represents the gene expression level from each source. This method has a significant meaning for the screening of disease-related genes, clinical early diagnosis and the preparation of specific medicine for the treatment of disease. | 11-20-2008 |
| 20080286767 | Method of Analyzing Dna Sequence Using Field-Effect Device, and Base Sequence Analyzer - Since conventional DNA sequence analyzing technologies are based on the fundamental principle of fluorescent detection, expensive, complex optical systems and laser sources have been necessary. | 11-20-2008 |
| 20080286768 | Sequencing a Polymer Molecule - A method for sequencing a target polymer molecule comprises the steps of: (i) treating the target polymer with an agent that degrades sequentially at least one end of the target polymer; (ii) converting at least a portion of the degraded end of different degraded polymers into a readable signal sequence, and labeling each of said degraded polymers with a tag that represents the relative order of degradation; (iii) determining the sequence of the readable signal sequence; and (iv) determining the sequence of the target polymer using the sequence data obtained in step (iii) and the identification of each associated tag. | 11-20-2008 |
| 20080286769 | Method of Quantitative and/or Comparative Measurement of Mrna Expression Levels in Small Biological Samples - The present invention provides a method for quantitative and/or comparative assessment of the relative amounts of mRNA transcripts present in a cell or tissue sample. In the method reverse transcription of the mRNA contained in the sample is first carried out using sequence-modifying primers for one or several genes in the same reaction to obtain a pool of sequence-modified cDNA molecules. After completion of the reverse transcription redundant sequence-modifying primers are removed or inactivated. This step is followed by a step of co-amplifying the sequence-modified cDNA templates with a reference DNA template in individual gene-specific amplification reactions. By quantitatively measuring the amounts and determining the relative levels of the amplification products derived from sequence-modified cDNA and reference DNA templates, a gene-specific cDNA over DNA ratio is obtained in each of the individual amplification reactions. Finally, by combining the ratios obtained, a sample-specific profile can be generated, which reflects the relative amounts of mRNA transcripts originally present in the sample. | 11-20-2008 |
| 20080286770 | DNA Molecules Encoding L-Glutamate-Gated Chloride Channels From Rhipicephalus Sanguineus - The present invention relates in part to isolated nucleic acid molecules (polynucleotides) which encode | 11-20-2008 |
| 20080286771 | Method of Predicting the Responsiveness of a Tumour to Erbb Receptor Drugs - The invention relates to a method of selecting a mammal having or suspected of having a tumour for treatment with an erbB receptor drug which comprises testing a biological sample from the mammal for expression of anyone of the genes listed in Table 1 or 2 as defined herein whereby to predict an increased likelihood of response to the erbB receptor drug. Preferred genes include anyone of NES, GSPT2, ETR101, TAZ, CHST7, DNAJC3, NPAS2, PIN1, TCEA2, VAMP4, DAPK1, DAPK2, MLLT3, TNNC1, KIAA0931, ACOX2, EMP1, SLC20A1, SPRY2 or PGM1. | 11-20-2008 |
| 20080286772 | Method for Direct Amplification from Crude Nucleic Acid Samples - The present teachings relate to improved methods, kits, and reaction mixtures for amplifying nucleic acids. In some embodiments a novel direct buffer formulation is provided which allows for the direct amplification of the nucleic acids in a crude sample with minimal sample purification. | 11-20-2008 |
| 20080286773 | Method for Typing an Individual Using Short Tandem Repeat (Str) Loci of the Genomic Dna - The present invention relates to a novel STR typing strategy, which allows the simultaneous amplification and subsequent analysis of several (e.g. eleven) polymorphic systems with amplicon sizes of less than 270 bp. Thereby, after a PCR amplification the multiplex reaction is divided into two sets of STR multiplexes and analyzed separately. This multiplex system was particularly developed and tested for use in forensic investigations, where only limited amounts of DNA or only highly degraded DNA is available, for example, when the DNA is isolated from the roots of telogen hair. | 11-20-2008 |
| 20080286774 | Real-time individualized therapy evaluation - A method of individually optimizing drug therapy to a patient that includes the steps of administering a dose of a radiolabeled drug or fluorescent tags or drugs with inherent fluorescent properties to the patient in connection with chemotherapy, collecting a sample from the patient, analyzing the sample producing an analysis, and using the analysis for developing a model for the patient. | 11-20-2008 |
| 20080286775 | METHODS OF NONSPECIFIC TARGET CAPTURE OF NUCLEIC ACIDS - Methods for capturing a target nucleic acid from a sample by using a capture probe that binds nonspecifically to the target nucleic acid and binds specifically to an immobilized probe via a specific binding pair that has one member on the capture probe and one member on the immobilized probe are disclosed. Compositions that include a capture probe that binds nonspecifically to a target nucleic acid and specifically to an immobilized probe via binding of members of a specific binding pair in a solution phase of a reaction mixture are disclosed. | 11-20-2008 |
| 20080286776 | Methods and Compositions for Assessment of Pulmonary Function and Disorders - The present invention provides methods for the assessment of risk of developing lung cancer in smokers and non-smokers using analysis of genetic polymorphisms. The present invention also relates to the use of genetic polymorphisms in assessing a subject's risk of developing lung cancer, and the suitability of a subject for an intervention in respect of lung cancer. Nucleotide probes and primers, kits, and microarrays suitable for such assessment are also provided. | 11-20-2008 |
| 20080286777 | Method of Determining the Diversity of T Lymphocytes in a Biological Sample - The invention relates to the field comprising the diagnosis of possible immune system disorders and the analysis of immune responses. In particular, the invention relates to a method of determining the diversity of T lymphocytes in a biological sample, based on the molecular analysis of the structure of the junctions resulting from the recombination rearrangement V(D)J of element δRec-1 with an AJ gene. More specifically, the invention relates to a method of analyzing the combinatorial diversity and/or the junctional diversity of the excision circles (TREC) resulting from the rearrangement of δRec-1, in order to determine the heterogeneity of a given population of T lymphocytes. | 11-20-2008 |
| 20080286778 | Method for Investigating Cytosine Methylations in Dna - The invention relates to a method for sensitively and specifically detecting cytosine methylations. For this purpose, DNA is first analysed by reacting with the aid of a methylation specific restriction enzyme. In such a way, the background DNA is removed from a reaction preparation. At a next step, a specific conversion of a non-methylated cytosine is carried out, while a methylated cytosine remains unchanged. The converted DNA can be analysed according to different methods, in particular by means of real time PCR method. | 11-20-2008 |
| 20080286779 | Host Cells Containing Multiple Integrating Vectors - The present invention relates to the production of proteins in host cells, and more particularly to host cells containing multiple integrated copies of an integrating vector. Suitable integrating vectors for use in the present invention include retrovirus vectors, lentivirus vectors, transposon vectors, and adeno-associated virus vectors. Methods are provided in which the host cells are prepared by using the integrating vectors at a high multiplicity of infection. The host cells are useful for producing pharmaceutical proteins, variants of proteins for use in screening assays, and for direct use in high throughput screening. | 11-20-2008 |
| 20080286780 | Novel compositions and methods for the identification, assessment, prevention and therapy of human cancers - The present invention is directed to the identification of markers that can be used to determine whether tumors are sensitive or resistant to a therapeutic agent. The present invention is also directed to the identification of therapeutic targets. The invention features a number of “sensitivity markers.” These are markers that are expressed in most or all cell lines that are sensitive to treatment with an agent and which are not expressed (or are expressed at a rather low level) in cells that are resistant to treatment with that agent. The invention also features a number of “resistance markers.” These are markers that are expressed in most or all cell lines that are resistant to treatment with an agent and which are not expressed (or are expressed at a rather low level) in cells that are sensitive to treatment with that agent. The invention also features marker sets that can predict patients that are likely to respond or not to respond to an agent. | 11-20-2008 |
| 20080286781 | Compositions, kits, and methods for identification, assessment, prevention, and therapy of cervical cancer - The invention relates to nucleic acid molecules and proteins associated with cervical cancer including pre-malignant conditions such as dysplasia. Compositions, kits, and methods for detecting, characterizing, preventing, and treating human cervical cancers are also provided. | 11-20-2008 |
| 20080286782 | ANALYSIS OF DNA SAMPLES - The invention provides an improved method for obtaining information about DNA analysis of samples of uncertain origin by establishing the likelihood that they arose in certain manners compared with other possible manners. In this way all of the analysis information is taken into account and likelihood ratios are provided to express the results. The invention is particularly useful in analysing small DNA samples or DNA samples where the contribution from one or more sources is small. | 11-20-2008 |
| 20080286783 | NOVEL METHOD OF DETECTING GENETIC POLYMORPHISM - The present invention provides a novel polymorphism detecting method suitable for the detection and identification of copy number variation. | 11-20-2008 |
| 20080286784 | Method for Detection of DNA Methyltransferase RNA in Plasma and Serum - The methods of the invention detect in a qualitative or quantitative fashion DNA methyltransferase RNA in blood plasma, serum, and other bodily fluids. The inventive methods are useful for aiding detection, diagnosis, monitoring, treatment, or evaluation of neoplastic disease, and for identifying individuals who have a predisposition to disease or who might benefit from further evaluation, monitoring or therapy. | 11-20-2008 |
| 20080286785 | Method to predict or monitor the response of a patient to an erbb receptor drug - The invention provides a method of detecting ErbB receptor mutations comprising the steps of providing a bio-fluid sample from a patient; extracting DNA from said sample; and screening said DNA for the presence of one or more mutations that alter tyrosine kinase activity in the receptor. | 11-20-2008 |
| 20080286786 | Methods of preparing nucleic acid for detection - Methods of preparing nucleic acid from polysaccharide-containing samples for detection by providing one or more glycosidases to the sample to degrade polysaccharides are provided. The nucleic acids can further be extracted from the sample. The method is particularly useful for detecting nucleic acid in samples with high starch content. | 11-20-2008 |
| 20080286787 | High Throughput Method of DNA Methylation Haplotyping - Particular aspects provide novel, high-throughput methods to quantify DNA methylation (e.g., at a single-base resolution) in an allele-specific manner. The methods comprise use of an allele-specific sequence polymorphism (e.g., allele-specific single nucleotide polymorphism; SNP) in sufficient proximity to a CpG methylation site to provide for distinguishing the methylation levels between two alleles. In particular aspects, after bisulfite modification, the genomic DNA region is PCR-amplified, and the product subjected to allele-specific pyrosequencing, and the percentage of methylation determined based on the percentage of cytosine to thymidine conversion. In further embodiments, MethyLight™ is used after bisulfite treatment. The inventive methodology has, for example, substantial utility for affording quantitative analyses in the regulation of analyses of X-inactivation, the allele-specific expression of genes (e.g., in the immune system) and junk DNA, etc., and in classifying an individual as to whether they have loss of imprinting (LOI). | 11-20-2008 |
| 20080286788 | NUCLEIC ACID LIGANDS TO COMPLEX TARGETS AND USES THEREOF - The present invention relates to a method for isolating a pool of nucleic acid ligands capable of binding to one or more target molecules in a complex mixture. | 11-20-2008 |
| 20080286789 | GENOMIC POLYMORPHISM FOR PREDICTING THERAPEUTIC RESPONSE - The present invention relates to the use of genomic polymorphism to provide individualized therapeutic regimens to treat patients suffering from diseases such as cancer. The invention discloses methods for determining the efficacy or choice of chemotherapeutic drugs and regimens for use in treating a diseased patient by associating genomic polymorphism with the effectiveness of the drugs or regimens, or by associating genomic polymorphism with the intratumoral expression of a gene whereby the gene expression affects effectiveness of the drugs or regimens. In particular, the present invention provides novel methods for screening therapeutic regimens, which comprise determining a patient's genotype at a tandemly repeated 28 base pair region in the thymidilate synthase (TS) gene's 5′ untranslated region (UTR). Patients homozygous for a triple repeat will be least successfully treated with a thymidylate synthase directed drug, while those heterozygous for a triple and a double repeat will be more successfully treated, and those homozygous for a double repeat will be even more successfully treated. Those patients homozygous for the double repeat will likely suffer the least side effects from thymidylate synthase directed drugs such as 5-FU. | 11-20-2008 |
| 20080286790 | Method for Diagnosing Overactive Bladder - Techniques for diagnosing overactive bladder (OAB) in a patient are provided. For example, a technique for diagnosing overactive bladder in a patient includes the step of obtaining peripheral blood mononuclear cells (PBMC) from the patient to provide a reporter function in the patient. | 11-20-2008 |
| 20080286791 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 2 and mutated sequences thereof. | 11-20-2008 |
| 20080286792 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 3 and mutated sequences thereof. | 11-20-2008 |
| 20080286793 | METHOD OF ANALYSIS OF PRIMARY STRUCTURAL CHANGE OF NUCLEIC ACID - The object of the present invention is to offer a method for analyzing primary structure change of nucleic acid which has quantifiability, which has a high degree of sensitivity and reproducibility, and which can be conducted rapidly and at low cost. The method of analysis of primary structural change of nucleic acid of the present invention includes a process for obtaining a reference nucleic acid having a standard sequence and a target sequence, each sequence being bound by a first ligand that differs depending on type of the sequence to which it binds, and being bound by a second ligand; a process for specifically binding first ligands to receptors supported on a carrier, thereby immobilizing standard sequences and target sequences on the carrier; a process for specifically binding labeled receptors to the second ligands in said nucleic acids which have been immobilized; a process for detecting labels to detect standard sequences and target sequences; a process for obtaining the ratio of standard sequences in the reference nucleic acid and subject nucleic acid, calculating a coefficient which corrects detection values of target sequences, and conducting correction; and a process for confirming an increase/decrease in the quantity of target sequences in the subject nucleic acid relative to target sequences in the reference nucleic acid. | 11-20-2008 |
| 20080286794 | METHODS AND PRIMERS FOR DIAGNOSING IDIOPATHIC CONGENITAL CENTRAL HYPOVENTILATION SYNDROME - The present invention provides assays and kits for diagnosing idiopathic congenital central hypoventilation syndrome. The present assays and kits focus on the second polyalanine repeat of the PHOX2b gene or gene product, which is normally 20 residues in length. A polyalanine repeat 25 to 33 residues in length is strongly correlated with idiopathic congenital central hypoventilation syndrome. | 11-20-2008 |
| 20080286795 | Method of nucleic acid amplification - A nucleic acid molecule can be annealed to an appropriate immobilized primer. The primer can then be extended and the molecule and the primer can be separated from one another. The extended primer can then be annealed to another immobilized primer and the other primer can be extended. Both extended primers can then be separated from one another and can be used to provided further extended primers. The process can be repeated to provide amplified, immobilized nucleic acid molecules. These can be used for many different purposes, including sequencing, screening, diagnosis, in situ nucleic acid synthesis, monitoring gene expression, nucleic acid fingerprinting, etc. | 11-20-2008 |
| 20080286796 | Genetic polymorphisms associated with neurodegenerative diseases, methods of detection and uses thereof - The present invention is based on the discovery of genetic polymorphisms that are associated with neurodegenerative disease, particularly Alzheimer's disease and Parkinson's disease. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, variant proteins encoded by such nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and proteins, and methods of using the nucleic acid and proteins as well as methods of using reagents for their detection. | 11-20-2008 |
| 20080286797 | Accurate identification of organisms based on individual information content - An improved method for specific identification of any organisms by DNA hybridization or amplification is disclosed. Oligonucleotides are designed based on information analysis of sequences from a large number of related species. Oligonucleotide sequences that have the maximal specificity to certain nucleic acids from a particular species (or set of species) or type strain are selected for hybridization or amplification using DNA from the target organism. The presence or absence of a PCR or hybridization product may be used to identify the target organism. The resulting PCR products may also be compared with a DNA sequence database to obtain the identity of the organisms. The methods may prove useful in areas where rapid and accurate identification of an organism is desirable, such as in a hospital where identification of infectious agents may be critical, in the ethanol or beer industry where certain bacteria may be detrimental to the manufacturing process, or in the porcine industry where identification of different type strains of the porcine reproductive and respiratory syndrome virus (PRRV) is important for disease prevention. | 11-20-2008 |
| 20080286798 | CONTROLS FOR PRIMERS IN MULTIPLEX AMPLIFICATION REACTIONS - The present invention provides methods and compositions for confirming the integrity of primers and other components of amplification reactions, including multiplex amplification reactions. | 11-20-2008 |
| 20080286799 | METHODS OF AND KITS AND COMPOSITIONS FOR DIAGNOSING COLORECTAL TUMORS... - In vitro methods of determining whether or not an individual has metastasized colorectal cancer cells are disclosed. In vitro methods of determining whether or not tumor cells are colorectal in origin are disclosed. In vitro kits for practicing the methods of the invention and to reagents and compositions useful to practice the methods, for example as components in such in vitro kits of the invention are provided. Methods of and kits and compositions for analyzing tissue samples from the colon tissue to evaluate the extent of metastasis of colorectal tumor cells are disclosed. | 11-20-2008 |
| 20080286800 | Method and system for biopsy and analysis of a body tissue - Methods and systems for performing biopsies and diagnosing tumors and suspect masses within the body. A biopsy sample is frozen while still in the body of a patient, then removed from the patient, stored frozen, and subject to IHC, microarray or other analysis to determine the amount, type or presence of signaling substances within the tumor or suspect mass. | 11-20-2008 |
| 20080286801 | Method for the analysis of differential expression in colorectal cancer - A method for the analysis of differential expression in colorectal cancer based on the variation in the expression levels of genes encoding for proteins forming part of the condensin complex or associated proteins that occurs in patients with the disease and that can be used as markers for the diagnosis of the cancers, as well as for the prevention and treatment thereof. | 11-20-2008 |
| 20080293043 | Method of Detecting Metastisizing Cancer Cells Originating in Stomach Cancer - Metastatic cancer cells originating from gastric cancer are detected by a method comprising the step of collecting a biological sample from a subject, the step of detecting the presence of at least either aldehyde dehydrogenase or dopa decarboxylase in the biological sample of the subject, and the step of determining that the possibility of containing metastatic cancer cells originating from the gastric cancer in the sample is high when at least either aldehyde dehydrogenase or dopa decarboxylase is present. By the use of these as markers for metastatic cancer cells originating from gastric cancer, the presence or absence of peritoneal metastasis in a gastric cancer patient can be detected rapidly and reliably, and data important for deciding whether intraperitoneal cancer chemotherapy should be applied is provided. | 11-27-2008 |
| 20080293044 | Melks as Modifiers of the Rac Pathway and Methods of Use - Human MELK genes are identified as modulators of the RAC pathway, and thus are therapeutic targets for disorders associated with defective RAC function. Methods for identifying modulators of RAC, comprising screening for agents that modulate the activity of MELK are provided. | 11-27-2008 |
| 20080293045 | Recombinase Polymerase Amplification - This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further disclosed are conditions to enable real-time monitoring of RPA reactions, methods to regulate RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carryover contamination, and methods to employ sequence-specific third ‘specificity’ probes. Further described are novel properties and approaches for use of probes monitored by light in dynamic recombination environments. | 11-27-2008 |
| 20080293046 | RNA detection assays - The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. | 11-27-2008 |
| 20080293047 | Method for the Diagnosis of Aspirin Intolerance - The present invention relates to a method for the diagnosis of aspirin intolerance based on a biological sample from a patient, characterized in that it comprises the following steps:
| 11-27-2008 |
| 20080293048 | METHODS AND KITS BASED ON UGT1A7 PROMOTER POLYMORPHISM - The present invention relates to methods for predicting the efficacy, safety and toxicity of substances, e.g. of drugs and prodrugs. Furthermore, the present invention relates to a method for the stratification of mammalians for the treatment of a disease. Moreover, the present invention provides for kits and its use for determining the efficacy, safety and toxicity of substances, in particular of drugs and prodrugs. The present invention allows for the selection of therapeutic regimens utilizing host genetic information, including gene sequence variances. The methods for identification of the specific DNA sequence variations according to the present invention include both in vitro and in vivo approaches. | 11-27-2008 |
| 20080293049 | Methods, Kids and Polynucleotides for Simultaneously Diagnosing Viruses - The present invention provides a simultaneous method for diagnosing HBV, HCV and HIV of a suspected patient. The present invention further provides different primer sets and specific probes for HBV, HCV and HIV. The present invention also provides a kit for simultaneous diagnosing of HBV, HCV and HIV. | 11-27-2008 |
| 20080293050 | Gene analysis for determination of a treatment characteristic - An apparatus, device, methods, computer program product, and systems are described that determine at least one amino acid sequence alteration of an amino acid sequence of a disease associated polypeptide, relative to an amino acid sequence of a corresponding non-disease associated polypeptide, identify a sub-sequence in the amino acid sequence of the disease associated polypeptide in which the amino acid sequence alteration occurs, relate the sub-sequence in the amino acid sequence of the disease associated polypeptide to a corresponding sub-sequence of the amino acid sequence of the corresponding non-disease associated polypeptide, and determine a treatment characteristic, based on the relating. | 11-27-2008 |
| 20080293051 | PROXIMITY LIGATION ASSAY - The present invention relates to compositions and methods for sensitive, rapid and convenient assays to detect and/or quantify one or more target using ribonucleic acid as probes, wherein the method includes binding a first and a second ribonucleic acid probe, each of which binds specifically to the target, wherein the first and second probes each comprise a ribonucleic acid tail; ligating the first and second ribonucleic acids tails thereby producing a ligated ribonucleic acid template; and performing amplification of the ribonucleic acid template across the first and second ribonucleic acids. | 11-27-2008 |
| 20080293052 | SYSTEM AND METHOD FOR AUTHENTICATING SPORTS IDENTIFICATION GOODS - A method for authenticating and verifying garment to be genuine is described. The method for authenticating a garment comprises applying a particular nucleic acid material/marker associated with a particular sequence of nucleic acid bases to a dye or paint and applying the marker to the garment. The method also comprises collecting a sample from the garment and verifying the garment is genuine by detecting the particular nucleic acid material on or within the garment. | 11-27-2008 |
| 20080293053 | shRNA Materials and Methods of Using Same for Inhibition of DKK-1 - Methods and compositions for the treatment of soft tissue cancer are described. More specifically, the invention demonstrates that inhibiting or otherwise decreasing the activity of DKK-1 using shRNA or siRNA molecules will be effective at reducing the cancer phenotype of prostate cancer cells. | 11-27-2008 |
| 20080293054 | Hmgcr isoforms in Prediction of Efficacy and Identification of Cholesterol-Modulating Compounds - The present invention provides methods for assessing a subject's responsiveness to a HMGCR inhibitor therapy, and selection of a HMGCR inhibitor therapy based upon such methods. The invention further provides methods for identifying agents that modulate HMGCR activity, e.g., through modulating HMGCR mRNA splicing, while avoiding elevation of the statin-resistant isoform of HMGCR. | 11-27-2008 |
| 20080293055 | K-ras mutations and anti-EGFr antibody therapy - The present application relates to K-ras mutations, to polynucleotides encoding mutant K-ras polypeptides, and to methods of identifying K-ras mutations. The present application also relates to methods of diagnosing cancer; and methods and kits for predicting the usefulness of anti-EGFr specific binding agents in the treatment of tumors. | 11-27-2008 |
| 20080293056 | METHOD FOR PREPARING CANCER STEM CELLS - The present invention provides a method for preparing cancer stem cells including the step of subjecting normal cells to Ras activation and p53 deficiency; the cancer stem cells prepared by the preparation method; a method for screening a cancer stem cell-targeting substance and a method for screening an anti-cancer substance using the cancer stem cells; a method for treating a cancer comprising administering to a patient the substances obtainable by the screening methods; and a diagnostic method for cancers including the step of detecting proteins specifically expressed in the cancer stem cells or mRNAs of the protein. | 11-27-2008 |
| 20080293057 | DETECTION OF UNSPECIFIED GENETICALLY MODIFIED ORGANISM (GMO) ON MICRO-ARRAYS - The present invention is related to a method, kit and computer program for detecting the presence in a sample of an unspecified Genetically Modified Organism (GMO). | 11-27-2008 |
| 20080293058 | Association of GSTM1 with autism and assays and methods based thereon - The present invention provides novel markers and assays for autism based on the association of GSTM1 with autism. The invention relates to the use and application of as a susceptibility marker for autism. GSTM1 may be combined with other markers in methods and assays for diagnosis, prenatal diagnosis, and assessment of autism. The invention further relates to a likelihood ratio test. In addition, the present invention discloses a novel method for identifying individuals who are genetically susceptible to have offspring with autism wherein the genotype of GSTM1, alone or in combination with other genetic markers, is determined. | 11-27-2008 |
| 20080293059 | Method of Identifying Induced Variability in in Vitro Cultures - The subject of the present invention is a method of identifying variability induced in in vitro cultures. The subject of the present invention may be used in the production of genetically stable lines, eg. of doubled haploids (determination of their genetic-apigenetic variability level), as well as facilitating a broadly understood, selection process of plant material in in vitro culture, previously unused in practice, for cultivation purposes, as well as the possibility of designing molecular tool for the identification of epigenetic characteristics for culturing purposes. | 11-27-2008 |
| 20080293060 | Methods and Compositions for Identification of Hydrocarbon Response, Transport and Biosynthesis Genes - Disclosed is a method using an alkane response element (ARE) from, e.g., | 11-27-2008 |
| 20080293061 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 4 and mutated sequences thereof. | 11-27-2008 |
| 20080293062 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 4 and mutated sequences thereof. | 11-27-2008 |
| 20080293063 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 4 and mutated sequences thereof. | 11-27-2008 |
| 20080293064 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 2 and mutated sequences thereof. | 11-27-2008 |
| 20080293065 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 2 and mutated sequences thereof. | 11-27-2008 |
| 20080293066 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 3 and mutated sequences thereof. | 11-27-2008 |
| 20080293067 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 2 and mutated sequences thereof. | 11-27-2008 |
| 20080293068 | METHODS FOR ENGINEERING POLYPEPTIDE VARIANTS VIA SOMATIC HYPERMUTATION AND POLYPEPTIDE MADE THEREBY - Methods using somatic hypermutation (SHM) for producing polypeptide and nucleic acid variants, and nucleic acids encoding such polypeptide variants are disclosed. Such variants may have desired properties. Also disclosed are novel polypeptides, such as improved fluorescent proteins, produced by the novel methods, and nucleic acids, vectors, and host cells comprising such vectors. | 11-27-2008 |
| 20080293069 | RAPID PROGNOSTIC ASSAY FOR MALIGNANCIES TREATED WITH EPIDERMAL GROWTH FACTOR RECEPTOR - A molecular assay for determining the sensitivity or resistance of malignancies to chemotherapy prior to initiation of chemotherapy and which also allows for monitoring the therapeutic effects of the chemotherapy during treatment. The molecular assay measures tumor response to therapy with EGFR modulators and utilizes tumor mRNA as a starting material and a quantitative measurement of c-fos expression as an analytical endpoint. | 11-27-2008 |
| 20080293070 | Markers for Memory T Cells and Uses Thereof - Methods, uses, products and kits are described relating to monitoring, assessing and modulating immune function and more particularly memory T cell function. Methods of identifying agents for such modulation are also described, as well as uses of such agents for modulating immune function. | 11-27-2008 |
| 20080293071 | Sequencing and Genotyping Using Reversibly Terminating Nucleotides - The invention provides a method of determining the nucleotide sequence of a target nucleic acid using a reversibly terminating nucleotide that is modified at the 2′ position. | 11-27-2008 |
| 20080293072 | METHOD FOR ENUMERATION OF MAMMALIAN MICRONUCLEATED ERYTHROCYTE POPULATIONS, WHILE DISTINGUISHING PLATELETS AND/OR PLATELET-ASSOCIATED AGGREGATES - A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample. In particular, the use of the second antibody prevents interference by platelet-associated aggregates in the scoring procedures. | 11-27-2008 |
| 20080299546 | Canine Cd20 Gene - It is intended to clarify the CD20 amino acid sequence and its gene sequence which are essentially required in constructing an anti-CD20 antibody useful in treating animal malignant lymphoma. It is also intended to provide a method of diagnosing canine malignant lymphoma by using the gene sequence. Using monocytes in canine blood as a sample, mRNA is obtained and the full base sequence of canine CD20 gene (SEQ ID NO:2) is determined. Based on this sequence, its amino acid sequence (SEQ ID NO:1) is determined. Comparing the homologies with human and mouse CD20 genes and amino acid sequences, it is identified as canine CD20 gene. Moreover, a primer specific to the canine gene is constructed and the expression of the CD20 gene in a sample is examined, thereby giving a method of diagnosing canine B lymphocyte-origin malignant lymphoma. | 12-04-2008 |
| 20080299547 | NOVEL STRA6 POLYPEPTIDES - The present invention is directed to novel polypeptides having sequence similarity to Stra6, a murine retinoic acid responsive protein, and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. | 12-04-2008 |
| 20080299548 | Gene Expressed Specifically in Es Cells and utilization of the Same - The present invention relates to an ES cell detection marker containing a polynucleotide derived from any one of ECAT15-1 gene, ECAT15-2 gene, ECAT16 gene, Rnf17 gene and LOC380905(TDRD4) gene. | 12-04-2008 |
| 20080299549 | Methods and compositions for the detection of a nucleic acid by a cleavage reaction - The invention provides methods, compositions and kits for generating a signal indicative of the presence of a target nucleic acid sequence in a sample comprising forming a cleavage structure by incubating a sample comprising a target nucleic acid sequence with upstream and downstream oligonucleotides, and cleaving the cleavage structure with a nuclease to generate a signal. The presence of a detectable signal is indicative of the presence of a target nucleic acid sequence and a non-invasive cleavage structure. | 12-04-2008 |
| 20080299550 | Methods and Kits For the Prediction of Therapeutic Success and Recurrence Free Survival In Cancer Therapy - The invention provides novel compositions, methods and uses, for the prediction, diagnosis, prognosis, prevention and treatment of malignant neoplasia and breast cancer. The invention further relates to genes that are differentially expressed in breast tissue of breast cancer patients versus those of normal “healthy” tissue. Differentially expressed genes for the identification of patients which are likely to respond to chemotherapy are also provided. | 12-04-2008 |
| 20080299551 | METHYLATION OF ESTROGEN RECEPTOR ALPHA AND USES THEREOF - Methods for diagnosis, prognosis, and treatment of cancer based on the methylation status of the ER-α gene promoter are disclosed. Methylation of the ER-α gene promoter is indicative of cancer and unfavorable prognosis. The cancer can be treated with a demethylation agent. | 12-04-2008 |
| 20080299552 | Method For Determining The Function Of Nucleic Acid Sequences And Expression Products Coded Thereby - The invention relates to a method for the investigation and determination of the function of nucleic acid sequences and nucleic acid expression products by the introduction of RNA into host cells. | 12-04-2008 |
| 20080299553 | Oligonucleotide Probes for the Genomic Typifying of Erythrocyte Systems, Methods and Relative Diagnostic Kits - The invention relates to oligonucleotide probes for the genomic typifying of erythrocyte systems, relative methods and diagnostic kits. | 12-04-2008 |
| 20080299554 | Genetic polymorphisms associated with liver fibrosis, methods of detection and uses thereof - The present invention is based on the discovery of genetic polymorphisms that are associated with liver fibrosis and related pathologies. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, including groups of nucleic acid molecules that may be used as a signature marker set, variant proteins encoded by such nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and proteins, and methods of using the nucleic acid and proteins as well as methods of using reagents for their detection. | 12-04-2008 |
| 20080299555 | Multicolor chromogenic detection of biomarkers - The present invention provides compositions, kits, assembles of articles and methodology for detecting multiple target molecules in a sample, such as in a tissue sample. In particular, site-specific deposition of elemental metal is used in conjunction with other means of detection, such as other chromogenic, radioactive, chemiluminescent and fluorescent labeling, to simultaneously detect multiple targets, such a gene, a protein, and a chromosome, in a biological sample. More particularly the multiple targets may be labeled with the specifically deposited metal and other chromogenic labels to allow chromogenic immunohistochemical (IHC) detection in situ by using bright field light microscope. | 12-04-2008 |
| 20080299557 | Sample Lysis and Coating of Reaction Surface - The present invention provides copolymers that facilitate nucleic acid analysis, compositions that comprise such copolymers, and methods for making or using such copolymers. | 12-04-2008 |
| 20080299558 | METHOD FOR DETECTING NUCLEIC ACID - The present invention provides a method for detecting a nucleic acid by specific binding between ligand and receptor, in particular, a method of detecting a SNP by the specific binding between a ligand and receptor. The present invention also provides a method for detecting a nucleic acid that is simpler, requiring only a single measurement operation, and shorter in measurement period. The present invention also provides a highly sensitive and highly accurate method for detecting a nucleic acid by using a specific binding reaction between a ligand and receptor in a reaction at the interface of a liquid and solid. The present invention also detects a nucleic acid by using a coagulation reaction of dispersible particles. | 12-04-2008 |
| 20080299559 | METHODS FOR AUTHENTICATING ARTICLES WITH OPTICAL REPORTERS - This invention pertains to methods for authenticating an article comprising tagging the article with light emitting optical reporter particles, and more specifically tagging the articles with up-converting phosphor particles (UCP), linked to nucleic acids of detectable sequence. | 12-04-2008 |
| 20080299560 | Nucleophosmin protein (NPM) mutants, corresponding gene sequences and uses thereof - The invention relates to new nucleophosmin protein (NPM) mutants, corresponding gene sequences and relative uses thereof for diagnosis, monitoring of minimal residual disease; prognostic evaluation and therapy of the acute myeloid leukaemia (AML) | 12-04-2008 |
| 20080299561 | Novel therapeutic targets in cancer - The present invention relates to novel sequences for use in detection, diagnosis and treatment of cancers, especially lymphomas. The invention provides cancer-associated (CA) polynucleotide sequences whose expression is associated with cancer. The present invention provides CA polypeptides associated with cancer that are present on the cell surface and present novel therapeutic targets against cancer. The present invention further provides diagnostic compositions and methods for the detection of cancer. The present invention provides monoclonal and polyclonal antibodies specific for the CA polypeptides. The present invention also provides diagnostic tools and therapeutic compositions and methods for screening, prevention and treatment of cancer. | 12-04-2008 |
| 20080299562 | NUCLEIC ACID-BASED TESTS FOR RHD TYPING, GENDER DETERMINATION AND NUCLEIC ACID QUANTIFICATION - The invention in part provides nucleic acid-based assays, which are particularly useful for non-invasive prenatal testing. The invention in part provides compositions and methods for RhD typing, detecting the presence of fetal nucleic in a sample, determining the relative amount of fetal nucleic acid in a sample and determining the sex of a fetus, wherein each of the assays may be performed alone or in combination. | 12-04-2008 |
| 20080299563 | HUMAN MAP2 GENE PROMOTER - The human MAP2 gene promoter as well as various fragments thereof are disclosed. Nucleic acids and host cells that contain the promoter sequences are also disclosed. Further disclosed are various methods involving the use of these sequences. | 12-04-2008 |
| 20080299564 | Control of Preservation By Biomarkers - The invention lies in the field of microbiology, more particularly in the field of (food) preservation and testing of viability of microbiological spores. It is shown that measurement of the integrity of both rRNA and mRNA in spores is an accurate indicator of viability. Nucleotide assays then form a significant improvement over the state of the art assays for viability. | 12-04-2008 |
| 20080299565 | Probe for Nucleic Acid Sequencing and Methods of Use - A nanoprobe for sequencing of nucleic acid molecules is provided, as well as methods for using the nanoprobe. In particular examples, the probe includes a polymerizing agent and one or more molecular linkers that carry a chemical moiety capable of reversibly binding to the template strand of a nucleic acid molecule, without being detached from the linker, by specifically binding with a complementary nucleotide in the target nucleic acid molecule. The reversible binding of the chemical moiety on the linker with a complementary nucleotide in the target nucleic acid molecule is indicated by emission of a characteristic signal that indicates pairing of the chemical moiety on the linker with its complementary nucleotide. An example of such a chemical moiety is a nonhydrolyzable nucleotide analog. In particular examples, the polymerizing agent and the chemical moiety are associated with a tag, such as a donor fluorophore and acceptor fluorophore characteristic of the particular type of chemical moiety. | 12-04-2008 |
| 20080299566 | Methylation of Gene Promoters as a Predictor of Effectiveness of Therapy - The present invention provides methods for identifying, diagnosing, evaluating or monitoring a disease state in a subject comprising identifying the methylation status of a panel of genes in the subject. The present invention also relates to identifying, diagnosing, evaluating or monitoring the responsiveness of a subject to a therapeutic regimen, with the methods comprising determining the methylation status of a panel of genes in the subject. | 12-04-2008 |
| 20080299567 | PRIMER AND PROBE SEQUENCES FOR DETECTING CHLAMYDIA TRACHOMATIS - The present invention relates to primers and probes that can be used in various assays to detect a new strain of | 12-04-2008 |
| 20080299568 | MATERIALS AND METHODS FOR DETECTION OF HEPATITIS C VIRUS - Disclosed are methods and compositions for detecting hepatitis C virus (HCV) in a sample. Typically, the methods include amplifying nucleic acids and detecting signals, such as signals emitted from fluorophores. The signals may be correlated to the presence and/or quantity of the hepatitis C viral genome in the sample. In some embodiments, primers are provided that specifically hybridize to the 3′ non-coding (NC) region of the HCV genome. | 12-04-2008 |
| 20080299569 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 2 and mutated sequences thereof. | 12-04-2008 |
| 20080299570 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 3 and mutated sequences thereof. | 12-04-2008 |
| 20080299571 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 4 and mutated sequences thereof. | 12-04-2008 |
| 20080299572 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 3 and mutated sequences thereof. | 12-04-2008 |
| 20080299573 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 2 and mutated sequences thereof. | 12-04-2008 |
| 20080299574 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 4 and mutated sequences thereof. | 12-04-2008 |
| 20080299575 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 2 and mutated sequences thereof. | 12-04-2008 |
| 20080299576 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 2 and mutated sequences thereof. | 12-04-2008 |
| 20080299577 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 2 and mutated sequences thereof. | 12-04-2008 |
| 20080299578 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 3 and mutated sequences thereof. | 12-04-2008 |
| 20080299579 | NOVEL CANCER MARKER AND USE THEREOF - This invention provides a method for cancer diagnosis comprising measuring an endogenous antisense RNA whose relative expression to a sense RNA changes cancer-specifically in RNA-containing samples collected from a mammal. Also provided are endogenous antisense RNAs useful as cancer markers, and cancer diagnostic reagents containing the same. | 12-04-2008 |
| 20080299580 | Targeted integration into the PPP1R12C locus - Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into the human PPP1R12C locus, for example, for expression of a polypeptide of interest. | 12-04-2008 |
| 20080299581 | Screening for expressible transfectants in a eukaryotic system - The present invention relates to the field of molecular cloning and to the field of expression cloning in higher, eukaryotic cells. In particular, the present invention relates to a method for fast and reliable identification of vectors and vector DNA which will be capable of providing a desired expression product if a eukaryotic host cell is transfected with the vector DNA. | 12-04-2008 |
| 20080299582 | Culture System for Rapid Expansion of Human Embryonic Stem Cells - This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy. | 12-04-2008 |
| 20080299583 | METHOD AND SYSTEM FOR ANALYZING REACTIONS USING AN INFORMATION SYSTEM - A method and system for determining the quantity of an analyte initially present in a chemical and or biological reaction as well as a computer implemented method and system to automate portions of the analysis comprising mathematical or graphical analysis of an amplification reaction. | 12-04-2008 |
| 20080305470 | Nucleic Acid Sequencing - A method for determining a target nucleic acid sequence, wherein the target nucleic acid sequence is comprised in a preparation comprising a non-target nucleic acid sequence, the target nucleic acid sequence and the non-target nucleic acid sequence each having a first region of common sequence upstream of a first region of dissimilar sequence upstream of a second region of dissimilar sequence, the method comprising:
| 12-11-2008 |
| 20080305471 | Optoelectronic device and method of fabricating the same - A modified isolated polypeptide comprising an amino acid sequence encoding a photocatalytic unit of a photosynthetic organism being capable of covalent attachment to a solid surface and having a photocatalytic activity when attached thereto is disclosed. | 12-11-2008 |
| 20080305472 | Method of detecting influenza bacillus, primer set for detection of influenza bacillus and kit for detection of influenza bacillus - The present invention provides a method of detecting | 12-11-2008 |
| 20080305473 | Propagation of primary cells - The present invention provides a method of propagating cells of interest obtained from a biological specimen by enriching the cells under conditions that maintain sufficient cell viability; and propagating the cells under conditions effective to allow cell viability, proliferation and integrity. | 12-11-2008 |
| 20080305474 | Method and Apparatus for Using SLC2A10 Genetic Polymorphisms for Determining Peripheral Vascular Disease in Patients with Type-2 Diabetes - Recent data indicate that a loss-of-function mutation of the SLC2A10 gene causes arterial tortuosity syndrome (ATS) via upregulation of the TGF-β pathway in the arterial wall, a mechanism possibly causing vascular changes associated with diabetes. It is determined that SLC2A10 (Solute carrier family 2, facilitated glucose transporter, member 10) genetic polymorphism is associated with peripheral vascular disease (PVD) in patients with type 2 diabetes. | 12-11-2008 |
| 20080305475 | MICROBIAL COMMUNITY ANALYSIS - The present invention provides a method which can enhance the precision of identification of bacterial species using a T-RFLP method and achieve, by itself, the identification of bacteria constituting a bacterial flora and the tracing of distribution changes thereof. The present invention provides a method for analyzing a bacterial community including: amplifying DNAs extracted from a bacterial community by PCR using 16S rRNA genes as templates and fluorescently labeled primers; cleaving the amplification products with a restriction enzyme to thereby obtain sample PCR fragments; electrophoresing the sample PCR fragments together with size standard PCR fragments; and comparing the mobilities thereof to thereby determine the sizes of the sample PCR fragments, wherein PCR fragments amplified by using, as a template, a 16S rRNA gene derived from a bacterium contained in the bacterial community to be analyzed are used as the size standards. | 12-11-2008 |
| 20080305476 | Immunoassay Methods - The invention relates to a method of detecting a disease state or disease susceptibility in a mammalian subject which comprises detecting an antibody in a test sample comprising a bodily fluid from said mammalian subject wherein said antibody is a biological marker of a disease state or disease susceptibility, the method comprising: (a) contacting said test sample with a plurality of different amounts of an antigen specific for said antibody, (b) detecting the amount of specific binding between said antibody and said antigen, (c) plotting or calculating a curve of the amount of said specific binding versus the amount of antigen for each amount of antigen used in step (a) and (d) determining the presence or absence of said disease state or disease susceptibility based upon the amount of specific binding between said antibody and said antigen at each different antigen concentration used. | 12-11-2008 |
| 20080305477 | Novel Iminecalixarene Derivatives and Aminocalixarene Derivatives, Method of Preparation Thereof, and Self-Assembled Monolayer Prepared by the Method, Fixing Method of Oligo-Dna By Using the Self-Assembled Monolayer, and Oligo-Dna Chip Prepared By the Method - The present invention relates to novel iminecalixarene derivatives, method of preparation thereof, and self-assembled monolayer prepared by the method, fixing method of oligo-DNA by using the self-assembled monolayer, and oligo-DNA chip prepared by the method. Also, the present invention relates to novel aminocalixarene derivatives, method of preparation thereof, and self-assembled monolayer prepared by the method, fixing method of oligo-DNA wherein the oligo-DNA is voluntarily fixed by molecular recognition on said self-assembled monolayer in a liquid phase, and oligo-DNA chip prepared by the method. | 12-11-2008 |
| 20080305478 | Processes Using Dual Specificity Oligonucleotide and Dual Specificity Oligonucleotide - The present invention relates to various processes by a template-dependent extension reaction using a dual specificity oligonucleotide and a dual specificity oligonucleotide composed of three different Tm portions therefor. Demonstrated in the present invention are the features of the dual specificity oligonucleotide, which are high hybridization specificity and mismatch tolerance. | 12-11-2008 |
| 20080305479 | DETECTION AND QUANTIFICATION OF BIOMOLECULES USING MASS SPECTROMETRY - The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and release labels for detection by mass spectrometry. This process is easily incorporated into a PCR amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids. | 12-11-2008 |
| 20080305480 | NOVEL TUMOR MARKER - The present invention concerns a gene product largely homologous to the epithelial growth factor receptor (EGFR). It further refers to mRNA coding for such epithelial growth factor receptor. The present invention provides such an epithelial growth factor receptor which is characterized in that either exons 12 to 14 or exons 12 to 15 are deleted. These novel variants of the epithelial growth factor receptor can be used for a diagnosis, stratification, therapy guidance of a tumor or therapy guidance of tumor surgery. | 12-11-2008 |
| 20080305481 | SYSTEMS AND METHODS FOR MULTIPLEX ANALYSIS OF PCR IN REAL TIME - The present invention provides methods and systems for real-time measurements of PCR with multiplexing capability. Certain embodiments relate to methods and systems that use fluorescently encoded superparamagnetic microspheres for the immobilization of amplification products during the PCR process, and an imaging chamber of a measurement device that is also capable of controllable thermal cycling for assisting the PCR process. | 12-11-2008 |
| 20080305482 | METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION - Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample. | 12-11-2008 |
| 20080305483 | BIALLELIC MARKERS DERIVED FROM GENOMIC REGIONS CARRYING GENES INVOLVED IN ARACHIDONIC ACID METABOLISM - The invention provides polynucleotides including biallelic markers derived from genes involved in arachidonic acid metabolism and from genomic regions flanking those genes. Primers hybridizing to regions flanking these biallelic markers are also provided. This invention also provides polynucleotides and methods suitable for genotyping a nucleic acid containing sample for one or more biallelic markers of the invention. Further, the invention provides methods to detect a statistical correlation between a biallelic marker allele and a phenotype and/or between a biallelic marker haplotype and a phenotype. | 12-11-2008 |
| 20080305484 | Genetic methods for speciating Campylobacter - Species-specific identification of | 12-11-2008 |
| 20080305485 | GENETIC MARKER FOR INCREASED RISK FOR OBESITY-RELATED DISORDERS - The present invention relates to methods of determining an increased risk of a subject to acquire a trait of an obesity disorder or an obesity disorder, with the method comprising determining the genetic sequence of at least one taste receptor gene in the subject and reviewing the test genetic sequence(s) for the presence of at least one risk allele associated with at least one taste receptor. The presence of at least one difference in the test genetic sequence(s) and the presence of a risk allele associated with the taste receptor(s) may indicate an increased risk of the subject acquiring a trait of an obesity disorder or an obesity disorder. | 12-11-2008 |
| 20080305486 | SIGNAL AMPLIFICATION USING CIRCULAR HAIRPIN PROBES - The present invention provides methods for detecting a target nucleic acid using a circular dual-hairpin probe that is formed upon the presence of the target nucleic acid. The detection methods find use in detecting the presence of antibody-antigen complexes and for detecting the binding of a ligand to its binding partner. Kits and reaction mixtures for performing the present methods are also provided. | 12-11-2008 |
| 20080305487 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 3 and mutated sequences thereof. | 12-11-2008 |
| 20080305488 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 3 and mutated sequences thereof. | 12-11-2008 |
| 20080305489 | NEAR-INFRARED DYES AS SURFACE ENHANCED RAMAN SCATTERING REPORTERS - Nanoparticles comprising surface-enhanced Raman scattering (SERS) reporter molecules of the formula A-Y and methods of their use are disclosed, wherein A is selected from the group consisting of: | 12-11-2008 |
| 20080305490 | AMPLIFICATION OF HUMAN MDM2 GENE IN HUMAN TUMORS - A human gene has been discovered which is genetically altered in human tumor cells. The genetic alteration is gene amplification and leads to a corresponding increase in gene products. Detecting that the gene, designated hMDM2, has become amplified or detecting increased expression of gene products is diagnostic of tumorigenesis. Human MD2 protein binds to human p53 and allows the cell to escape from p53-regulated growth. | 12-11-2008 |
| 20080305491 | MCM6 AND MCM7 MONOCLONAL ANTIBODIES AND METHODS FOR THEIR USE IN THE DETECTION OF CERVICAL DISEASE - Compositions and methods for diagnosing high-grade cervical disease in a patient sample are provided. The compositions include novel monoclonal antibodies, and variants and fragments thereof, that specifically bind to MCM6 or MCM7. Monoclonal antibodies having the binding characteristics of an MCM6 or MCM7 antibody of the invention are further provided. Hybridoma cell lines that produce an MCM6 or MCM7 monoclonal antibody of the invention are also disclosed herein. The compositions find use in practicing methods for diagnosing high-grade cervical disease comprising detecting overexpression of MCM6, MCM7, or both MCM6 and MCM7 in a cervical sample from a patient. Kits for practicing the methods of the invention are further provided. Polypeptides comprising the amino acid sequence for an MCM6 or an MCM7 epitope and methods of using these polypeptides in the production of antibodies are also encompassed by the present invention. | 12-11-2008 |
| 20080305492 | DETECTION OF GLEEVEC RESISTANCE - The present invention relates to isolated polypeptides which comprise an amino acid sequence consisting of a mutated functional Abl kinase domain, said mutated functional kinase domain being resistant to inhibition of its tyrosine kinase activity by N-[4-methyl-3-(4-pyridin-3-yl-pyrimidin-2-ylamino)-phenyl]-4-(4-methyl-piperazin-1-ylmethyl)-benzamide or a salt thereof, to the use of such polypeptides to screen for compounds which inhibit the tyrosine kinase activity of such polypeptides, to nucleic acid molecules encoding such polypeptides, to recombinant vectors and host cells comprising such nucleic acid molecules and to the use of such nucleic acid molecules in the production of such polypeptides for use in screening for compounds which inhibit the tyrosine kinase activity of such polypeptides. | 12-11-2008 |
| 20080311562 | Nucleic Acid Sequencing - A method for determining a target nucleic acid sequence, wherein the target nucleic acid sequence is comprised in a preparation comprising a non-target nucleic acid sequence, the target nucleic acid sequence and the non-target nucleic acid sequence each having a first region of common sequence upstream of a first region of dissimilar sequence upstream of a second region of dissimilar sequence, the method comprising: | 12-18-2008 |
| 20080311563 | Methods for selecting medications - Methods for selecting a medication for a patient are described that include determining the patient's genotype for a panel of genes and selecting the medication based on the genotype. Articles of manufacture also are provided that include nucleic acid molecules for detecting alleles of genes encoding drug metabolizing enzymes and genes encoding products involved in neurotransmission. | 12-18-2008 |
| 20080311564 | Sequences and Methods for Detection of Cytomegalovirus - Amplification primers and methods for specific amplification and detection of a CMV target are disclosed. The primer-target binding sequences are useful for amplification and detection of the CMV target in a variety of amplification and detection reactions. | 12-18-2008 |
| 20080311565 | Methods and Kits for Detecting Germ Cell Genomic Instability - Disclosed are methods for detecting microsatellite instability in the germ line of males, methods of assessing risk for developing testicular cancer, methods of evaluating the microsatellite stability of putative cancer or precancerous cells or a tumor, methods for evaluating germ cells for exposure to mutagens, and kits for use in the methods of the invention. | 12-18-2008 |
| 20080311566 | Method for Quantification of Methylated Dna - The present invention relates to a method for the quantification of methylated cytosines in DNA. In the first step of the invention unmethylated cytosines in the DNA to be analysed are chemically converted into uracil while 5-methylcytosines remain unchanged. In a second step the converted DNA is amplified methylation specifically in a real time PCR using a methylation specific probe. Finally the amount of uniformly methylated DNA is calculated by combining criteria derived from the shape of the real time curve and from the signal intensity. The method is preferably used for diagnosis and/or prognosis of adverse events for individuals, for distinguishing cell types and tissues, or for investigating cell differentiation. | 12-18-2008 |
| 20080311567 | Tumor Markers for Use in the Diagnosis of Colorectal Carcinomas and/or Metastases Originating Therefrom - The invention relates to a method (i) for detecting a carcinoma, especially an adenocarcinoma, preferably a gastrointestinal carcinoma and more preferably a colorectal carcinoma, (ii) for predicting metastases, preferably liver metastases, depending on a primary colon carcinoma and/or (iii) for predicting the response of metastases to a 5-fluorouracil-containing chemotherapy. The inventive method comprises determining a gene expression profile of 120 marker genes or a selection thereof. | 12-18-2008 |
| 20080311568 | Compositions and Methods for Nucleic Acid Analysis of Sequences with Insertions or Deletions - Contemplated methods and kits include a plurality of single stranded oligonucleotides that comprise a discriminating sequence that is encompassed on one end by a label and on the other end by a unique tag sequence. In one preferred aspect, the discriminating sequence is an RNA repeat unit, forms a heteroduplex with a complementary DNA repeat unit of the target nucleic acid, and the discriminating agent is RNaseH. Using such systems, the number of repeat units can be simply identified by hydrolysis of the discriminating sequence where such sequence is adjacent to a predetermined number of DNA repeat units in the single stranded oligonucleotide. | 12-18-2008 |
| 20080311569 | METHOD FOR QUANTITATIVE MEASUREMENT OF GENE EXPRESSION FOR INDENTIFYING INDIVIDUALS AT RISK FOR BRONCHOGENIC CARCINOMA - A method measure expression of multiple target genes in a progenitor cell for bronchogenic carcinoma comprising the use of reverse transcription-polymerase chain reaction (RT-PCR) to allow simultaneous expression measurement of the multiple target genes is disclosed. | 12-18-2008 |
| 20080311570 | CANCER SCREENING METHOD - A method for screening cancer comprises the following steps: (1) providing a test specimen; (2) detecting the methylation state of the CpG sequence in at least one target gene within the genomic DNA of the test specimen, wherein the target genes is consisted of SOX1, PAX1, LMX1A, NKX6-1, WT1 and ONECUT1; and (3) determining whether there is cancer or cancerous pathological change in the specimen based on the presence or absence of the methylation state in the target gene; wherein method for detecting methylation state is methylation-specific PCR (MSP), quantitative methylation-specific PCR (QMSP), bisulfite sequencing (BS), microarrays, mass spectrometer, denaturing high-performance liquid chromatography (DHPLC), and pyrosequencing. | 12-18-2008 |
| 20080311571 | Nucleic Acid Fragments for Detecting Nucleic Acid and Method for Detecting Nucleic Acid - A nucleic acid fragment set according to the present invention comprises a plural number of nucleic acid fragments capable of individually hybridizing with a plural number of target sequences, in which each nucleic acid fragment has a region ligatable with each other and the affinity between such ligatable regions is adjusted to a higher level than the affinity between the target sequence and the nucleic acid fragment. The nucleic acid fragment according to the present invention is for use in a nucleic acid fragment kit. The nucleic acid fragment of the present invention can be used as a probe for detecting nucleic acid or a competitor for detecting nucleic acid. According to the present invention, human leukocyte antigen (HLA) genes, T-cell receptor genes, red blood cell group determining genes, and Rh antigen genes, and the like can be detected at a high accuracy level. | 12-18-2008 |
| 20080311572 | Methods and Compositions For Correlating Ccl3l1/Ccr5 Genotypes With Disorders - The present invention provides compositions and methods for identifying persons at an increased risk of infection by, transmission of, or accelerated progression of a disease caused by an HIV-1 virus. Diagnostic and therapeutic kits are also provided. | 12-18-2008 |
| 20080311573 | Compositions, kits, and methods for identification, assessment, prevention, and therapy of breast cancer - The invention relates to newly discovered nucleic acid molecules and proteins associated with breast cancer. Compositions, kits, and methods for detecting, characterizing, preventing, and treating human breast cancers are provided. | 12-18-2008 |
| 20080311574 | Novel Missense Mutations and Single Nucleotide Polymorphisms in the Rabphillin-3A-Like Gene and Uses Thereof - The invention relates to methods and compositions of matter for determining or predicting aggressiveness of a subject's tumor, for determining a subject's predisposition to cancer, for diagnosing cancer in a subject, and for selecting a therapy for a subject with cancer. Also provided are methods and compositions of matter for determining a Rabphillin-3A-Like gene genotype in a subject and for characterizing a Rabphillin-3A-Like gene in a subject. | 12-18-2008 |
| 20080311575 | Modulation of Muci-Dependent Anti-Estrogen Resistance - The present invention provides methods for identification and use of compounds that modulate the association of MUC1 with estrogen receptors and thereby antagonize MUC1-related resistance to anti-estrogen treatment. | 12-18-2008 |
| 20080311576 | Replication competent hepatitis C virus and methods of use - The present invention provides a replication competent hepatitis C virus that includes a heterologous polynucleotide. The invention also includes methods for modifying a hepatitis C virus polynucleotide, selecting a replication competent hepatitis C virus polynucleotide, detecting a replication competent hepatitis C virus polynucleotide, and identifying a compound that inhibits replication of a hepatitis C virus polynucleotide. | 12-18-2008 |
| 20080311577 | Method for the Identification of Sulfo-Oxidizing Bacteria and for the Monitoring of Elemental Sulfur in the Environment - A method is described for the identification of sulfooxidizing bacteria comprising the extraction of the DNA from environmental samples and the subsequent identification of at least one fragment of the Thio 16S gene or SoxB gene present in these bacteria. The method can be used for determining the level of elemental sulfur in samples of soil. | 12-18-2008 |
| 20080311578 | System for screening eukaryotic membrane proteins - The present invention provides methods and compositions for expressing eukaryotic membrane proteins. | 12-18-2008 |
| 20080311579 | Hybridisation beacon and method of rapid sequence detection and discrimination - A method for detecting specific DNA sequences and discriminating single nucleotide polymorphisms (SNPs) using fluorescently labelled oligonucleotide probes is disclosed. Oligonucleotide probes are labelled with reporter molecules preferentially attached to an internal nucleotide residue. The fluorescence emission of oligonucleotide probes varies significantly when in single-stranded and double-stranded states despite the absence of quencher moieties, allowing reliable detection of complementary DNA targets. The melting temperature of probe/target duplexes permits discrimination of targets that differ by as little as a single nucleotide residue, such that polymorphic targets may be discriminated by fluorescence quantitation and Tm. The hybridisation probes of this invention have been demonstrated to accurately identify homozygous and heterozygous samples using a single fluorescent oligonucleotide and direct investigation of saliva with hybridisation probes permits ultra-rapid genotypic analysis within 35-40 minutes. Target detection and SNP discrimination assays have been achieved in homogeneous, heterogeneous, ‘real-time’ and solid-phase formats. | 12-18-2008 |
| 20080311580 | Novel genes encoding proteins involved in proanthocyanidin synthesis - This inventions provides an isolated protein or polypeptide having activity in the synthesis of proanthocyanidin (PA) polymer from epicatechin or catechin in plants, and which is not naturally regulated by the TT2 or TT8 regulators, or a fragment comprising at least about 10 contiguous amino acids derived from said protein or polypeptide, particularly TDS1, TDS2, TDS3, TDS4, TDS5, or TDS6 protein, or fragment thereof. | 12-18-2008 |
| 20080311581 | FUNCTIONAL POLYMORPHISMS OF THE INTERLEUKIN-1 LOCUS AFFECTING TRANSCRIPTION AND SUSCEPTIBILITY TO INFLAMMATORY AND INFECTIOUS DISEASES - The invention provides methods and reagents for detecting a polymorphism associated with in an upstream region of the interleukin-1β (IL-B) gene (IL-1B (−3737)) that affects transcription of the gene and susceptibility to inflammatory and infectious diseases such as diffuse coronary artery disease. | 12-18-2008 |
| 20080311582 | Methods Using Pores - The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto. | 12-18-2008 |
| 20080311583 | CHROMOSOME MANIPULATION METHOD - A universal method for chromosome modification (deletion of a desired DNA region) which does not require any special enzymes or sequences is provided. Further, a method for determining whether the DNA region to be deleted contains a gene essential for growth of the cell under certain culture conditions is provided by utilizing the chromosome modification method. | 12-18-2008 |
| 20080311584 | Methods for Detection of Mutations in Myostatin Variants - Methods for detecting allelic variants of the myostatin (growth and differentiation factor-8) gene are provided. Specifically provided are methods of identifying subjects having or having a predisposition for increased muscle mass as compared to subjects having wild-type myostatin. Increased muscle mass is particularly desirable for identification of animals used to produce food products, including bovine, porcine, ovine, avian and piscine species. | 12-18-2008 |
| 20080311585 | System and method for multiplex liquid handling - The present invention generally relates to microfabricated devices for carrying out and controlling chemical reactions and analysis. In particular, the present invention provides systems, methods, devices and computer software products related to multiplex liquid handling systems utilizing lab cards related to biological assays. | 12-18-2008 |
| 20080311586 | METHOD FOR MEASURING IN VIVO HEMATOTOXICITY WITH AN EMPHASIS ON RADIATION EXPOSURE ASSESSMENT - The present invention relates a method for assessing in vivo hematotoxicity. The method utilizes differential staining of nucleated and non-nucleated blood cells, and also differential labeling of cells with functional versus dysfunctional mitochondrial membrane potential. Quantitative analyses can be conducted on stained whole blood specimens, and is based on blood cells' fluorescent emission and light scatter properties following exposure to an excitatory light source. The ratio of certain cell populations can be readily measured. Furthermore, it is also possible to express cell population values in terms of number per unit volume. This invention can be used to evaluate the hematotoxicity of drugs, chemicals, radiation, and other exogenous agents, or the effects that a suspected protective agent may have on induced hematotoxicity. Furthermore, the matrix of measurements provided by this invention is useful in estimating radiation dose, i.e., retrospectively. Kits for practicing the invention are also disclosed. | 12-18-2008 |
| 20080311587 | Use of Genetic Information to Detect a Predisposition for Bone Density Conditions - The invention relates to kits and methods for assessing susceptibility of a human to an undesirable bone density condition, such as osteopenia and osteoporosis, and advising appropriate interventions. The methods involve contemporaneously assessing occurrence in the human's genome of a plurality of polymorphisms (e.g., single nucleotide polymorphisms) that occur in one or more genes associated with bone density regulation and that are associated with a disorder in humans. Preferred assessment and scoring methods are disclosed, as are kits for performing the methods. | 12-18-2008 |
| 20080318209 | Cjd Prion Testing - The present invention provides an assay method for detecting infectious prion protein in a sample from a mammalian subject, said method comprising: obtaining a prion protein containing sample from said subject; contacting said sample with an agent which serves to digest non-infectious prion protein and to partially digest infected prion protein to yield a prion protein polypeptide residue; contacting the digested sample with an antibody capable of binding to a poly peptide having the amino acid sequence Vc (Gly-Gly-Gly-Trp)-Gly-Gln-Gly-Gly-R | 12-25-2008 |
| 20080318210 | BIOINFORMATICALLY DETECTABLE GROUP OF NOVEL REGULATORY VIRAL AND VIRAL ASSOCIATED OLIGONUCLEOTIDES AND USES THEREOF - The present invention relates to a first group of novel viral and human associated oligonucleotides, here identified as Genomic Address Messenger or GAM oligonucleotide, and a second group of novel operon-like viral and human polynucleotides, here identified as Genomic Record or GR polynucleotide. GAM oligonucleotides selectively inhibit translation of known ‘target’ genes, many of which are known to be involved in various viral diseases. Nucleic acid molecules are provided respectively encoding 15484 GAM precursors oligonucleotides, and 699 GR polynucleotides, as are vectors and probes both comprising the nucleic acid molecules, and methods and systems for detecting GAM oligonucleotides and GR polynucleotides and specific functions and utilities thereof, for detecting expression of GAM oligonucleotides and GR polynucleotides, and for selectively enhancing and selectively inhibiting translation of the respective target genes thereof. | 12-25-2008 |
| 20080318212 | Orphan Receptor Tyrosine Kinase as a Target in Breast Cancer - Methods and materials relating to the orphan receptor tyrosine kinase (ROR1) are described. ROR1 exhibits restricted tissue expression in normal adult tissue and is overexpressed in certain breast cancer subtypes. ROR1 provides a diagnostic and/or therapeutic target for breast cancers. | 12-25-2008 |
| 20080318213 | Mass Spectrometer - A mass spectrometer and a method of spectrometry are disclosed wherein liquid chromatography mass spectral data are probabilistically clustered on the basis of mass to charge ratio and retention time. | 12-25-2008 |
| 20080318214 | Genome Analysis Method - This invention makes it possible to perform analysis for estimating the characteristics of a population using sample data. By obtaining sample data, embedding genetic (statistical) knowledge in a first and second state variable that have duality, and having the first and second state variables converge to the original value, the characteristics of the population of the sample data are estimated, and the estimated results of the characteristics of the population are output. By doing so, it is possible to perform analysis for estimating characteristics of a population using sample data. | 12-25-2008 |
| 20080318215 | APPARATUS, METHODS AND PRODUCTS FOR DETECTING GENETIC MUTATION - Methods for detecting genetic mutation allowing detection of very low frequency mutation. Methods comprise treating RNA:DNA heteroduplexes of interest with ribonuclease treatment coupled with DNA polymerase treatment. RNA:DNA heteroduplexes of interest are preferentially targeted for digestion by ribonuclease and subsequent sequence extension by DNA polymerase. Methods may be carried out partially or entirely manually, automatically, and combinations thereof. Methods may be performed wholly or partially in solution, on solid phase media, in large scale, adapted for high throughput analysis, and any combinations thereof. Apparatus and products for detecting genetic mutation. | 12-25-2008 |
| 20080318216 | Methods For Determining Presence of Cancer by Analyzing the Expression of Cdk9 and/or Cyclin T1 in Lymphoid Tissue - A method for determining presence of lymphoma in a patient is disclosed. A sample of bone marrow, thymus, spleen, lymph nodes, lymph and/or lymphocytes taken from the patient t is assayed to determine expression of CDK9 and CYCLIN T1. The presence of CDK9 and/or CYCLIN T1 is indicative of a lymphoma other than a mantle cell lymphoma or marginal zone lymphoma in the patient. | 12-25-2008 |
| 20080318217 | Identification of Gene Associated with Reading Disability and Uses Therefor - The present invention relates to identification of a human gene, DCDC2 (MIM: 605755), associated with susceptibility for developing reading disability (RD), which is useful in identifying or aiding in identifying individuals at risk for developing RD, as well as for diagnosing or aiding in the diagnosis of RD. | 12-25-2008 |
| 20080318218 | Compositions and Methods for Inferring an Adverse Effect in Response to a Drug Treatment - Methods are provided for inferring whether a subject to be treated with a drug such as a statin or an ACE inhibitor is likely to suffer an adverse effect due to the treatment. Also provided are compositions for practicing the methods. | 12-25-2008 |
| 20080318219 | METHODS AND COMPOSITIONS FOR PREDICTING DRUG RESPONSES - The present invention relates to methods and compositions for predicting drug responses. In particular, the present invention provides methods and compositions for determining individualized Warfarin dosages based on genotype of DNA polymorphisms and haplotypes derived from them in the VKORC1 gene. | 12-25-2008 |
| 20080318220 | METHOD FOR THE DIAGNOSIS AND/OR PROGNOSIS OF ALZHEIMER'S DISEASE - The present invention relates to a method for the diagnosis and/or prognosis of Alzheimer's disease by means of determining the ZMIZ1 gene expression level in a biological sample and comparing said level with a reference value, in which the alteration of said level is indicative of Alzheimer's disease. | 12-25-2008 |
| 20080318221 | METHOD FOR THE DIAGNOSIS AND/OR PROGNOSIS OF ALZHEIMER'S DISEASE - The present invention relates to a method for the diagnosis and/or prognosis of Alzheimer's disease by means of determining the DARC gene expression level in a biological sample and comparing said level with a reference value, in which the disturbance of said level is indicative of Alzheimer's disease. | 12-25-2008 |
| 20080318222 | Cell Based Methods And Systems For The Identification Of Rna Regulatory Sequences And Compounds That Modulate Their Functions - The present invention is directed to an in vivo method for screening for and/or identifying RNA regulatory sequences involved in translational control. The method includes: culturing cells including: (i) a translational system; (ii) a reporter mRNA; and (iii) an RNA test sequence under suitable conditions for translation of the reporter mRNA, wherein the RNA test sequence is not attached to the reporter mRNA. The method also includes measuring the effect of the test sequence on the translation of the reporter mRNA, wherein a test sequence that modifies the translation of the reporter mRNA includes an RNA regulatory element. The invention also provides methods and systems for screening for and/or identifying test compounds that modulate the regulatory activity of an RNA regulatory sequence. This method includes culturing cells including (i) a translation system; (ii) a reporter mRNA; and (iii) an RNA regulatory sequence in the presence of at least one test compound, wherein the RNA regulatory sequence is not attached to the reporter mRNA. The ability of the test compound to affect the interaction between the RNA regulatory sequence and at least one component of the translation system is sought to be determined. The method further includes monitoring for the effect of the test compound on the this interaction, wherein a compound that modifies this interaction is a drug candidate. | 12-25-2008 |
| 20080318223 | LARGE DELETIONS IN HUMAN BRCA1 GENE AND USE THEREOF - Large deletions have been identified in the BRCA1 gene in patients. The large deletions predispose the patients to breast cancer and ovarian cancer. Thus, methods for detecting the genetic variants are provided which can be used in detecting a predisposition to cancer. | 12-25-2008 |
| 20080318224 | LARGE DELETIONS IN HUMAN BRCA1 GENE AND USE THEREOF - Large deletions have been identified in the BRCA1 gene in patients. The large deletions predispose the patients to breast cancer and ovarian cancer. Thus, methods for detecting the genetic variants are provided which can be used in detecting a predisposition to cancer. | 12-25-2008 |
| 20080318225 | COMPOSITIONS AND METHODS FOR DETECTING CANCER - The present invention provides methods and compositions involving detecting the presence of and/or assessing the risk of cancer in a subject. These methods include methods of detecting and diagnosing cancer in an individual; methods of identifying individuals at risk of developing a cancer; and methods of staging a cancer. The methods generally involve detecting a palladin gene nucleotide sequence alteration that has been found to be associated with cancer and/or detecting a level of a palladin mRNA and/or protein in a biological sample. The present invention further provides nucleic acid probes, nucleic acid primers, and antibodies, as well as kits comprising one or more of the same, for use in a subject method. | 12-25-2008 |
| 20080318226 | Signal Amplification Method - Provided are a signal amplification method of improving signal sensitivity, qualifying properties and handling property in detection of a target gene by using a PALSAR method, a method of detecting a target gene by using the method, and an oligonucleotide probe to be used in the method. A signal amplification method in detection of a target gene using a polymer formed by the use of a plurality of kinds of oligonucleotide probes having complementary base sequence regions capable of hybridizing with each other, including labeling at least one of the plurality of kinds of oligonucleotide probes with acridinium ester for detection. | 12-25-2008 |
| 20080318227 | Intermediates and enzymes of the non-mevalonate isoprenoid pathway - The invention provides a protein in a form that is functional for the enzymatic conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate to 1-hydroxy-2-methyl-2-butenyl 4-diphosphate notably in its (E)-form of the non-mevalonate biosynthetic pathway to isoprenoids. The invention also provides a protein in a form that is functional for the enzymatic conversion of 1-hydroxy-2-methyl-2-butenyl 4-diphosphate, notably in its (E)-form, to isopentenyl diphosphate and/or dimethylallyl diphosphate. Further, screening methods for inhibitors of these proteins are provided. Further, 1-hydroxy-2-methyl-2-butenyl 4-diphosphate is provided and chemical and enzymatic methods of its preparation. | 12-25-2008 |
| 20080318228 | Biomarkers and methods for determining sensitivity to microtubule-stabilizing agents - Biomarkers that are useful for identifying a mammal that will respond therapeutically or is responding therapeutically to a method of treating cancer that comprises administering a microtubule-stabilizing agent. In one aspect, the cancer is breast cancer, and the microtubule-stabilizing agent is an epothilone or analog or derivative thereof, or ixabepilone. | 12-25-2008 |
| 20080318229 | Method for Diagnosing Neuro-Degenerative Disease - A method for the diagnosis of Alzheimer's Disease (AD) or Parkinson's Disease (PD), comprises measuring the level of expression of one or more AD markers (Table 1) or PD markers (Table 2) in a sample of platelets isolated from a person suspected of having AD or PD, and determining whether the levels of expression are altered compared to a control. | 12-25-2008 |
| 20080318230 | GENE EXPRESSION MARKERS FOR RESPONSE TO EGFR INHIBITOR DRUGS - The present invention concerns prognostic markers associated with cancer. In particular, the invention concerns prognostic methods based on the molecular characterization of gene expression in paraffin-embedded, fixed samples of cancer tissue, which allow a physician to predict whether a patient is likely to respond well to treatment with an EGFR inhibitor. | 12-25-2008 |
| 20080318231 | 14-3-3 ZETA OVER-EXPRESSION AS A POOR PROGNOSIS FACTOR, AND A THERAPEUTIC TARGET IN MULTIPLE CANCER TYPE - Methods of determining prognosis in a subject with a hyperproliferative disease, including determining expression and/or function of 14-3-3 zeta in the subject, are disclosed. Also disclosed are methods of making a pharmaceutical agent that modulates apoptosis, including the steps of obtaining one or more candidate, testing the one or more candidate substances to determine their ability to modulate the expression and/or function of 14-3-3 zeta, selecting a candidate substance determined to modulate the expression and/or function of 14-3-3 zeta, and making a pharmaceutical composition that includes the selected candidate substance. In addition, methods of treating a subject with a hyperproliferative disease, including making a pharmaceutical agent by the methods set forth herein, and administering the pharmaceutical agent to a subject, are disclosed. The hyperproliferative disease can be cancer, such as breast cancer. | 12-25-2008 |
| 20080318232 | Nucleic Acid Biosensor with Photoelectrochemical Amplification - The present invention relates to electrode systems, methods, apparatus and chips for ultrasensitive detection and quantification of nucleic acids using photoelectrochemical amplification. Upon hybridization of a target nucleic acid to a nucleic acid capture probe, a photoreporter comprising a threading bis-intercalator selectively binds to double-stranded nucleic acid. The stability and reversibility of the photoreporter binding activity provides for ultrasensitive detection of nucleic acid hybridization events. | 12-25-2008 |
| 20080318233 | Source tagging and normalization of DNA for parallel DNA sequencing, and direct measurement of mutation rates using the same - This invention allows efficient tagging and normalization of DNA molecules prior to pooling and characterization using parallel DNA sequencing (e.g., commercially available 454 sequencers). The invention provides novel ways to process independent DNA samples (sources) so that similar numbers of molecules from each source are represented in the pool (i.e., the pool is normalized for representation among the sources). These approaches would save researchers time and energy relative to approaches currently available. In other embodiments, the invention provides novel ways to process large numbers of independently derived DNA samples that can be uniquely tagged in ways that allow the source of the DNA (e.g., an individual) to be tracked. The invention also provides novel ways for processing DNA to consistently obtain various portions of the genome to compare DNA sequences and directly measure mutations using state of the art massively parallel DNA sequencing (e.g., commercially available 454 sequencers and others. The invention is useful for the production of diagnostic assays for individuals prior to reproduction (e.g., cancer survivors who wish to procreate) and also for diagnostics of cancer (thus affecting the choice of treatment for cancer). | 12-25-2008 |
| 20080318234 | Compositions and methods for diagnosing and treating cancer - The present invention relates to compositions and methods for treating, characterizing, and diagnosing cancer. In particular, the present invention provides gene expression profiles associated with solid tumor stem cells, as well as novel stem cell cancer gene signatures useful for the diagnosis, characterization, prognosis and treatment of solid tumor stem cells. | 12-25-2008 |
| 20080318235 | Chromosomal Analysis By Molecular Karyotyping - The invention provides a method of karyotyping (for example for the detection of trisomy) a target cell to detect chromosomal imbalance therein, the method comprising: (a) interrogating closely adjacent biallelic SNPs across the chromosome of the target cell (b) comparing the result at (a) with the SNP haplotype of paternal and maternal chromosomes to assemble a notional haplotype of target cell chromosomes of paternal origin and of maternal origin (c) assessing the notional SNP haplotype of target cell chromosomes of paternal origin and of maternal origin to detect aneuploidy of the chromosome in the target cell. Also provided are related computer-implemented embodiments and systems. | 12-25-2008 |
| 20080318236 | Cell Free Screening Assay and Methods of Use - Methods are disclosed for identifying agents for the treatment of cancer and/or Fanconi anemia using | 12-25-2008 |
| 20080318237 | METHODS OF DIAGNOSING ENDOMETRIOSIS - The present invention provides biomarkers for the diagnosis and prognosis of endometriosis. Generally, the methods of this invention find use in diagnosing or for providing a prognosis for endometriosis by detecting the expression levels of biomarkers, which are differentially expressed (up- or down-regulated) in endometrial cells from a patient with endometriosis. Similarly, these markers can be used to diagnose reduced fertility in a patient with endometriosis or to provide a prognosis for a fertility trial in a patient suffering from endometriosis. The present invention also provides methods of identifying a compound for treating or preventing endometriosis. Finally, the present invention provides kits for the diagnosis or prognosis of endometriosis. | 12-25-2008 |
| 20080318238 | METHOD OF DETECTING GENE MUTATION - DNA amplification and hybridization are successively carried out in a reaction system containing primers for the DNA amplification and hybridization probes, followed by detecting the hybrid in the reaction solution by affinity chromatography, wherein at least one of the primers to be used in the DNA amplification is labeled with a first labeling agent so that the amplified DNA will be labeled with the first labeling agent, a hybridization probe is labeled with a second labeling agent and contained in a reaction solution for effecting the DNA amplification, the base sequence of the hybridization probe is designed not to inhibit the DNA amplification, and a hybrid is detected by affinity chromatography with the use of the first and second labeling agents. | 12-25-2008 |
| 20080318239 | COMPOSITIONS AND METHODS FOR THE DIAGNOSIS AND TREATMENT OF TUMOR - The present invention is directed to compositions of matter useful for the diagnosis and treatment of tumor in mammals and to methods of using those compositions of matter for the same. | 12-25-2008 |
| 20080318240 | METHODS FOR UTILIZING ESR COPY NUMBER CHANGES IN BREAST CANCER TREATMENTS AND PROGNOSES - The present invention relates to methods for estimation of efficacy of therapeutic treatment of cancer patients, in particular breast cancer patients. The estimation is based on determining of the status of aberration of the estrogen receptor alpha gene (ESR1) in situ, and, optionally, the status of aberration of a gene related to ESR1. In particular, the invention relates to determining the presence or absence and, if present, the type of aberration, e.g. amplification, duplication, polyploidization, deletion or translocation of the ESR1 gene in the tumor cells of the patient. The invention further relates to a kit-in-parts comprising probes for the determining the status of aberration of ESR1 and ESR1-related genes in situ. | 12-25-2008 |
| 20080318241 | Methods and Systems for Detecting Antiangiogenesis - The present invention provides methods and systems for the detection of tumor vessel response to antiangiogenic therapies. The present invention also provides compositions and methods for therapeutic and research applications. In particular, the present invention provides systems and methods that employ CD26, HIF-1, and HIF-1 pathway components as biomarkers for monitoring antiangiogenic therapies and as therapeutic targets. | 12-25-2008 |
| 20080318242 | METHODS AND DEVICES FOR EARLY DETECTION OF CANCER CELLS AND TYPES THROUGH MICROMECHANICAL INTERACTIONS - Methods and devices for detecting a cancer cell and cancer cell types in a sample of a subject are provided. | 12-25-2008 |
| 20080318243 | DNA measuring system and method - The present invention provides a DNA sequencer using a FET sensor, capable of long-base decoding. Target DNAs are immobilized on the surfaces of spherical fine particles, the fine particles are disposed in the vicinity of metal electrodes each of which is connected electrically to a corresponding one of conductive wirings of the FET sensor and partly has a spherical surface capable of contacting with the fine particles, and the FET sensor detects a change in interfacial potential incident to an extension reaction of DNA molecules containing a hybridization of the target DNA and probe DNA. | 12-25-2008 |
| 20080318244 | Large-scale parallel nucleic acid analysis method - It is intended to provide a technique for amplifying, individually and in parallel, nucleic acids contained in a mixture of plural kinds of nucleic acid samples. The present invention provides a nucleic acid analysis method comprising amplification means, whereby amplification reaction is performed in a reaction solution comprising a homogeneous solvent and comprising at least plural template nucleic acids and solid phase carriers comprising one or more kinds of amplification probes immobilized on the surface, to prevent amplified products attributed to two or more template nucleic acids from being replicated in one solid phase carrier. According to the present invention, plural kinds of analyte nucleic acid samples in a mixed state can be amplified individually and in parallel. This method achieves one solid phase carrier-one nucleic acid. Therefore, a higher density of solid phase carriers with obtained amplified products is easily achieved, leading to improved throughput of amplified product analysis. Reactions in all the amplification reaction steps are performed under homogeneous solvent conditions. Therefore, the method of the present invention is performed by convenient procedures and as such, is suitable to automation. | 12-25-2008 |
| 20090004645 | Methods for Identifying Genes that Mediate a Response of a Living Cell to an Agent - In one aspect, the present invention provides methods for determining whether a gene mediates the response of a living cell to an agent. In another aspect, the present invention provides methods to identify a mammalian subject responsive to a KSP inhibitor. | 01-01-2009 |
| 20090004646 | Method for Quantifying Methylated Dna - The inventive method makes it possible to quantify methylated DNA by combining the restricted digestion and real-time PCR. For this purpose, the inventive method consists in isolating the examined DNA from a biological sample, in reacting the isolated AND with a methylation specific restriction enzyme, in amplifying by means of a real-time PCR, wherein the amplified products are formed only when the DNA is precut-off and, afterwards, in calculated the methylated and unmethylated DNA proportion in the initial sample with the aid of a reference measurement. Said method is particularly suitable for diagnosis and prognosis cancer and other diseases associated with a methylation state modification and for predicting the drug effects. | 01-01-2009 |
| 20090004647 | Method of Judging Grade of Malignancy of Carcinoma Cell - Means for easily determining the grade of malignancy of cancer cells. The amount of ATBF1 in the whole cell structure of test cancer cells separated from a living organism is detected, and on the basis of detection results, the grade of malignancy of test cancer cells is judged. Alternatively, the amount of ATBF1 in the nuclei of test cancer cells separated from a living organism is detected, and on the basis of detection results, the grade of malignancy of test cancer cells is judged. Still alternatively, the amount of ATBF1 in the cytoplasms of test cancer cells separated from a living organism is detected, and on the basis of detection results, the grade of malignancy of test cancer cells is judged. In a preferred from, at least one of (1) the amount of intranuclear presence and/or intracytoplasmic presence a region corresponding to exon 10 of an ATBF1 gene, (2) the amount of intranuclear presence and/or intracytoplasmic presence a region corresponding to exon 11 of an ATBF1 gene, and (3) the amount of intranuclear presence and/or intracytoplasmic presence a region corresponding to exon 3 of an ATBF1 gene is detected as the amount of ATBF1. | 01-01-2009 |
| 20090004648 | Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis of ovarian cancer - Novel markers for ovarian cancer that are both sensitive and accurate. These markers are overexpressed and/or differentially expressed in ovarian cancer specifically, as opposed to normal ovarian tissue. The measurement of these markers, alone or in combination, in patient samples provides information that the diagnostician can correlate with a probable diagnosis, in ovarian cancer. The markers of the present invention, alone or in combination, show a high degree of differential detection between ovarian cancer and non-cancerous states. | 01-01-2009 |
| 20090004649 | EVERNINOMICIN BIOSYNTHETIC GENES - This invention is directed to nucleic acids which encode the proteins that direct the synthesis of the orthosomycin everninomicin and to use of the nucleic acids and proteins to produce compounds exhibiting antibiotic activity based on the everninomycin structure. The DNA sequence for the gene clusters responsible for encoding everninomicin biosynthetic genes, which provide the machinery for producing everninomicin, are provided. Thus, this invention provides the nucleic acid sequences needed to synthesize novel everninomicin-related compounds based on everninomicin, arising from modifications of the DNA sequence designed to change glycosyl and modified orsellinic acid groups contained in everninomicin. A | 01-01-2009 |
| 20090004650 | Methods for detecting a Cyclophilin B SNP associated with Herda - This invention provides compositions and methods for identification of carriers of Hereditary Equine Regional Dermal Asthenia (HERDA) in equine species. In particular, this invention identifies a single nucleotide polymorphorism (SNP) in cyclophlin B that can be used to identify carriers of HERDA and individuals affected by HERDA. | 01-01-2009 |
| 20090004651 | Screening methods for compounds that modulate the activity of G-protein coupled receptors - The present invention relates to a screening system for modulators of GPCRs. Further it relates to recombinant vector systems for the heterologous expression of heterodimeric g-protein coupled receptors (GPCRs) in eucaryotic host cells. Preferably the functional expression of engineered GPCRs for the perception of sweet and L-amino acid taste or more preferably the use of said receptors for the identification of functional ligands is also encompassed. | 01-01-2009 |
| 20090004652 | Methods for identifying and using SNP panels - Described are methods for identifying single nucleotide polymorphism (SNPs) that are useful for analyzing genetic samples, and for using said SNPs to determine genetic identity of samples. | 01-01-2009 |
| 20090004653 | Sulfonated [8,9]benzophenoxazine dyes and the use of their labelled conjugates - Fluorescent, sulfonated 3,7-diamino-[8,9]benzophenoxazine dyes are provided that are especially useful for labelling biopolymers and other substrates. The dye-labelled conjugates can be used in a variety of contexts, including cell surface assays employing intact, live cells and in nucleic acid detection methods. The new dyes are water soluble and can be conjugated to a variety of substrates, such as polynucleotides, nucleosides, nucleotides, peptides, proteins, antibodies, carbohydrates, ligands, particles and surfaces. | 01-01-2009 |
| 20090004654 | METHOD FOR THE DETECTION OF BACTERIAL SPECIES OF THE GENERA ANAPLASMA/EHRLICHIA AND BARTONELLA - The present invention relates to a method for the detection and identification of bacterial species belonging to the genera | 01-01-2009 |
| 20090004655 | RNA BIOASSAY - The present invention relates to methods for evaluating the cell damaging potential of an agent by determining the ability of the agent to increase messenger RNA release in cells. | 01-01-2009 |
| 20090004656 | THERMOSTABLE POLYMERASES HAVING ALTERED FIDELITY AND METHODS OF IDENTIFYING AND USING SAME - The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant. | 01-01-2009 |
| 20090004657 | 7B2 Knockout transgenic animals as models of endocrine disease - In general, the invention features methods and uses for transposon-mediated gene targeting which greatly enhance the insertion and detection of desired genes in genomic exons by homologous recombination. The invention also features transgenic non-human mammals, and eukaryotic cells, wherein a gene encoding 7B2 protein is modified, as well as nucleic acid vectors capable of undergoing homologous recombination with an endogenous 7B2 gene in a cell. The invention also features transgenic non-human mammals as models of endocrine disorders, as well as methods for diagnosing and treating patients with endocrine disorders. | 01-01-2009 |
| 20090004658 | INTEGRIN ALPHA 7 MUTATIONS IN PROSTATE CANCER, LIVER CANCER, GLIOBLASTOMA MULTIFORME, AND LEIOMYOSARCOMA - Methods are provided for determining the presence of a cancer in a biological sample, such as a tissue biopsy. The methods comprise determining if integrin alpha 7 expression is decreased in the biopsy which is indicative of the presence of a cancer or likelihood of relapse of a cancer. This can be accomplished by determining if levels of integrin alpha 7 mRNA or protein are decreased as compared to a control. This also can be accomplished by determining if a mutation in the integrin alpha 7 gene is present in the biopsy. | 01-01-2009 |
| 20090004659 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 4 and mutated sequences thereof. | 01-01-2009 |
| 20090004660 | METHOD FOR DETECTING NEUROBLASTOMA AND ITS MALIGNANCY AND METHOD FOR SUPPRESSING THE SAME - An object of the present invention is to provide a method for detecting cancer and a cell growth inhibitor through identification of genes exhibiting characteristic behavior in the cases of cancer such as neuroblastoma. The present invention provides a method for detecting cancer, which comprises detecting canceration including malignancy of a specimen through detection of inactivation of a gene in the q22 region of chromosome 14 in the specimen. | 01-01-2009 |
| 20090004661 | METHOD OF GROWING MESENCHYMAL STEM CELLS FROM BONE MARROW - The present invention provides a method for culturing mesenchymal stem cells using cord blood serum, for therapeutic purposes in regenerative medicine; and in particular the present invention provides for the use of these cells in the treatment of PD, and the present invention has provided proliferation and neuronal differentiation of the MSCs in a xenofree culture system for clinical applications in a simple two step protocol, and the in vivo functional efficacy was tested in Parkinson's animal model. | 01-01-2009 |
| 20090004662 | METHOD AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION - The present teachings provide methods, compositions, and kits for nucleic acid amplification. In some embodiments of the present teachings, amplification reactions are performed with at least one high stability primer. In some embodiments, the present teachings provide a method comprising a high stability primer for amplification of a nucleic acid sequence in a sample comprising a target nucleic acid sequence and a PCR inhibitor. | 01-01-2009 |
| 20090004663 | Real-Time Assays Of Neuro-Humoral Factors To Assess Cardiovascular Stress - The present invention relates to rapid assays for neuro-humoral factors modulated in response to cardiovascular stress and integration of data obtained from such assays to provide profiles of response to cardiovascular stress that can guide therapy. | 01-01-2009 |
| 20090004664 | HCV NS3 replicon shuttle vectors - The present invention provides for novel HCV NS3 replicon shuttle vectors useful for cloning in HCV polynucleotide sequences from samples of HCV-infected patients and testing the resulting replicons for drug susceptibility. | 01-01-2009 |
| 20090004665 | METHODS AND COMPOSITIONS FOR ISOLATING NUCLEIC ACID SEQUENCE VARIANTS - The invention is drawn to isolating sequence variants of a genetic locus of interest using a modified iterative primer extension method. The nucleic acids analyzed are generally single stranded and have a reference sequence which is used as a basis for performing iterative single nucleotide extension reactions from a hybridized polymerization primer. The iterative polymerization reactions are configured such that polymerization of the strand will continue if the sequence of the nucleic acid being analyzed matches the reference sequence, whereas polymerization will be terminated if the nucleic acid being analyzed does not match the reference sequence. Nucleic acid strands that have mutations can be isolated using a variety of methods and sequenced to determine the precise identity of the mutation/polymorphism. By performing the method on both strands of the nucleic acid being analyzed, virtually all possible mutations can be identified. | 01-01-2009 |
| 20090004666 | METHOD OF DETECTING NUCLEOTIDE SEQUENCE WITH AN INTRAMOLECULAR PROBE - A nucleotide sequence-detecting method, including preparing a first intramolecular detecting sequence having a sequence complementary to a first sequence located at a 3′-side of the detecting site contained in the nucleotide sample and a second intramolecular detecting sequence having a sequence complementary to a second sequence located at a 5′-side of the detecting site, preparing a detecting chain containing a sequence of the detecting chain by connecting the first intramolecular detecting sequence to the 3′ terminal of the nucleotide sample and the second intramolecular detecting sequence to the 5′ terminal, allowing intramolecular hybridization at two positions of the detecting chain, connecting the 3′ terminal of the first intramolecular detecting sequence to the 5′ terminal of the second intramolecular detecting sequence, obtaining a cyclic structure, detecting the desired sequence in the nucleotide sample from the cyclic structure. | 01-01-2009 |
| 20090004667 | METHOD FOR GENERATING APTAMERS WITH IMPROVED OFF-RATES - The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently crosslinking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element. | 01-01-2009 |
| 20090004668 | Pre-miRNA loop-modulated target regulation - By employing essential nucleotides from both the stem and loop of precursor-miRNA, greater specificity is achieved as to the mRNAs that are repressed. It is found that besides the seed sequence of the stem of the pre-miRNA, nucleotides in the loop affect the activity and specificity of the cursor- and the processing and binding to target mRNA. By using both sequences in the natural pre-miRNA or modified mimetics, one can screen for cellular miRNA expression, modulate cell properties with greater specificity and investigate cellular activity as to phenotype and response to external stimuli in the presence and absence of target protein expression. | 01-01-2009 |
| 20090004669 | Methods of diagnosing pre-eclampsia or eclampsia - Disclosed herein are methods for diagnosing pre-eclampsia and eclampsia. Also disclosed herein are methods for treating pre-eclampsia and eclampsia using compounds that increase VEGF or PlGF levels or compounds that decrease sFlt-1 levels. Compounds that inhibit the binding of VEGF or PlGF to sFlt1- are also disclosed herein for the treatment of pre-eclampsia or eclampsia. | 01-01-2009 |
| 20090011405 | Modulating Screening Thresholds for N-Hybrid Screening - The present invention provides improved N-hybrid assays. In particular, the present invention provides an improved reverse N-hybrid assay comprising modulating the amount of a substrate of a reporter gene and/or the amount of a reporter gene thereby enhancing cell death in the absence of a peptide inhibitor of a DNA-protein or protein-protein interaction and/or enhancing cell survival in the presence of a peptide inhibitor of a DNA-protein or protein-protein interaction. Furthermore, the present invention provides an improved forward N-hybrid assay comprising modulating the amount of a reporter gene thereby enhancing cell death in the absence of a heterologous peptide or protein capable of binding to the DNA or protein in a cell and enhancing cell survival in the presence of a heterologous peptide or protein capable of binding to the DNA or protein in a cell. | 01-08-2009 |
| 20090011406 | Method of Judging Fatigue - A method of judging fatigue, which comprises judging fatigue by using, as an index, a value obtained by quantitatively analyzing an adenine nucleotide in a sample to be measured. | 01-08-2009 |
| 20090011407 | Polymorphic Cd24 Genotypes that are Predictive of Multiple Sclerosis Risk and Progression - An image data correction apparatus has a motion information acquisition section, a correction section, and a composition section. The motion information acquisition section acquires motion information indicating spatial distribution of the magnitude of motion, in actual space, of a to-be-imaged portion of a subject. Based on the motion information, the correction section performs correction, which is different from correction in a second region, in a first region of image data collected by a scan by magnetic resonance imaging. The composition section composes individual image data of the first region and the second region that are corrected by the correction section. | 01-08-2009 |
| 20090011408 | Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase - The invention relates to and methods for generating a signal indicative of the presence of a target nucleic acid in a sample. The compositions and methods include a reverse transcriptase, a nuclease, an upstream primer and downstream probe. | 01-08-2009 |
| 20090011409 | Antibodies Against Cells of Fetal Origin - This invention relates to antibodies that specific bind to fetal CD36+ cells in preference to binding to maternal CD36+ cells and methods for using these antibodies to detect and separate fetal cells from adult biological fluids including maternal peripheral blood. | 01-08-2009 |
| 20090011410 | METHOD FOR TAILORING ADMINISTRATION OF DRUGS BY QUANTITATION OF MRNA - The present invention discloses a method for tailoring drug protocols to individual patients based on the levels of marker mRNA measured in leukocytes after stimulation of whole blood of the patient with candidate drugs. A method of measuring a patient's responsiveness to a drug is disclosed that includes exposing whole blood of the patient to the drug for 7 hours or less; after the exposure, measuring the amount of an mRNA associated with an effect of the drug in blood cells; and identifying responsiveness to the drug based on the results of the measurement, wherein a change in the amount of the mRNA indicates the patient's responsiveness to the drug. The amount of mRNA measured in the blood cells may be compared with the level of mRNA present in the cells before exposure or with the level of mRNA present in cells exposed for the same amount of time to a control vehicle. Marker mRNAs useful in the present invention include mRNAs encoding the gene product of the p21, BAX, PUMA, NOXA, and IL-2 genes. The method may be employed for patients with, among other conditions, cancer or diseases or conditions requiring immunosuppression. | 01-08-2009 |
| 20090011411 | Method for Detecting and Quantifying Endogenous Wheat Dna Sequence - It is an object of the present invention to provide partial sequences of endogenous wheat DNA (genome) which are single copies and which allow wheat to be specifically detected without cross-reacting with other plants in PCR, and to provide primers for amplifying these partial sequences, along with a good method for detecting and quantifying endogenous DNA using these primers. The present invention provides, in a method for detecting or quantifying an endogenous wheat DNA sequence in a test sample by means of a polymerase chain reaction, a method comprising a step of using a nucleic acid in the test sample or a nucleic acid extracted from the test sample as a template to amplify the nucleic acid of a region comprising at least 80% or more of a nucleotide sequence represented by one of SEQ ID NO:1 through SEQ ID NO:7 using a primer pair capable of amplifying that region, and a step of detecting or quantifying the amplified nucleic acid. | 01-08-2009 |
| 20090011412 | HIP1 cancer markers - The present invention relates to compositions and methods for cancer diagnostics, including but not limited to, HIP | 01-08-2009 |
| 20090011413 | Method for screening colon cancer cells and gene set used for examination of colon cancer - Colon cancer cells in a sample are screened by analyzing the amount of expression of at least 2 or more genes or products thereof selected from the group of genes listed in Tables 1 and 30. As compared to conventional method, patients having colon cancer can be detected with higher accuracy. Colon cancer cells in stool are also screened by analyzing expression of genes selected from the group of genes listed in Table 37. | 01-08-2009 |
| 20090011414 | HUMAN AUTISM SUSCEPTIBILITY GENE ENCODING A KINASE AND USES THEREOF - The present invention discloses the identification of a human autism susceptibility gene, which can be used for the diagnosis, prevention and treatment of autism and related disorders, as well as for the screening of therapeutically active drugs. The invention more specifically discloses that the MARK1 gene on chromosome 1 and certain alleles thereof are related to susceptibility to autism and represent novel targets for therapeutic intervention. The present invention relates to particular mutations in the MARKI gene and expression products, as well as to diagnostic tools and kits based on these mutations. The invention can be used in the diagnosis of predisposition to, detection, prevention and/or treatment of Asperger syndrome, pervasive developmental disorder, mental retardation, anxiety, depression, attention deficit hyperactivity disorders, speech delay, epilepsy, metabolic disorder, immune disorder, bipolar disease and other psychiatric and neurological diseases including schizophrenia. | 01-08-2009 |
| 20090011415 | REGULATION OF NEURONAL EXPRESSION OF APOLIPOPROTEIN E - The present invention provides nucleic acids comprising a nucleotide sequence encoding an apolipoprotein E (apoE) splice variant, e.g., an unprocessed apoE, that retains intron 3; and vectors and host cells comprising same. The present invention further provides screening methods to identify agents that inhibit cleavage of intron-3 from the apoE splice variant. The present invention further provides methods of treating apoE-related neurological disorders, involving administering an agent that inhibits removal of intron-3 from a precursor apoE mRNA. | 01-08-2009 |
| 20090011416 | Random array DNA analysis by hybridization - The invention relates to methods and devices for analyzing single molecules, i.e. nucleic acids. Such single molecules may be derived from natural samples, such as cells, tissues, soil, air and water without separating or enriching individual components. In certain aspects of the invention, the methods and devices are useful in performing nucleic acid sequence analysis by probe hybridization. | 01-08-2009 |
| 20090011417 | Testing Device - The invention provides, in different aspects, a system, sample preparation device, sample processing cartridge, kit, methods of use, business methods, and computer program product. | 01-08-2009 |
| 20090011418 | FUNCTIONAL TOLL-LIKE RECEPTORS (TLR) ON MELANOCYTES AND MELANOMA CELLS AND USES THEREOF - The invention relates to a method of detecting Toll-like receptor (TLR) gene expression or protein activity in a melanocyte or melanoma cell. Also disclosed are a method of modulating TLR gene expression or protein activity in a melanocyte or melanoma cell by contacting the cell with a TLR modulating agent and a method of inhibiting melanoma cell migration (e.g., spreading) by contacting the cell with a TLR inhibitor. | 01-08-2009 |
| 20090011419 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 2 and mutated sequences thereof. | 01-08-2009 |
| 20090011420 | SPARSELY CROSS-LINKED NANOGELS: A NOVEL POLYMER STRUCTURE FOR MICROCHANNEL DNA SEQUENCING - The present invention is generally directed to novel polymeric materials for use in the electrophoretic separation of nucleic acids. In particular, the novel polymer materials are sparsely crosslinked nanogels, dissolved in an aqueous buffer to form solutions with moderate to high viscosity. The present invention further provides methods for generating such novel polymers, and related methods of their use. | 01-08-2009 |
| 20090011421 | SUSCEPTIBILITY TO BONE DAMAGE - In one aspect, the present invention provides methods for determining susceptibility to bone damage in a subject. In some embodiments, the methods comprise screening for polymorphisms in the MTHFR and collagen Iα1 genes that are associated with susceptibility to bone damage. In some embodiments, the methods comprise screening for elevated levels of homocysteine in a subject, wherein elevated levels of homocysteine are associated with an increased risk of bone damage. The methods of the invention may be used in predicting the response of a patient to treatment. Also provided are methods for prevention or reducing the risk of bone damage in a subject. | 01-08-2009 |
| 20090011422 | METHODS FOR CLONING SMALL RNA SPECIES - This invention pertains to methods for cloning microRNA (miRNA) and other small ribonucleic acid (RNA) species from relevant cell sources. | 01-08-2009 |
| 20090011423 | GENES FOR PROGNOSIS OF CANCER - To provide a novel method for determining the risk of lymph node metastasis of breast cancer uses as an index the difference in the expression levels of marker genes between metastatic breast cancer tissue (or cell) and non-metastatic breast cancer tissue (or cell). The method involves ( | 01-08-2009 |
| 20090011424 | Cancer-suppressing agents - It is an object of the present invention to provide a cancer-suppressing agent comprising a novel cancer-suppressing gene based on the discovery of such cancer-suppressing gene. The present invention provides a cancer-suppressing agent which comprises NR1I2 gene or a homologous gene thereof; and a cancer-suppressing agent which comprises NR1I2 protein or a homologous protein thereof. | 01-08-2009 |
| 20090017450 | Primer for detection of human papillomavirus - Disclosed are primers specific to the genome of HPV genotypes 11, 16, 18 and 31. Also disclosed are a kit for detecting the HPV genome comprising the primers and a method of detecting the HPV genome using the primers. | 01-15-2009 |
| 20090017451 | Generation of Recombinant Genes in Saccharomyces Cerevisiae - The present invention relates to methods for generating and detecting recombinant DNA sequences in | 01-15-2009 |
| 20090017452 | METHODS AND COMPOSITIONS RELATING TO THE PHARMACOGENETICS OF DIFFERENT GENE VARIANTS - The present invention is directed to methods and compositions for determining the presence or absence of polymorphisms within an ABCC2, UGT1A1, and/or SLCO1B1 gene and correlating these polymorphisms with activity levels of their gene products and making evaluations regarding the effect on their substrates, particularly those substrates that are drugs. In addition, there are methods and compositions of evaluating the risk of an individual for developing toxicity or adverse event(s) to an ABCC2, UGT1A1, and/or SLCO1B1 substrate. In some embodiments, the invention concerns methods and compositions for determining the presence or absence of ABCC2 3972C>T variant and predicting or anticipating the level of activity of ABCC2 and determining dosages of an ABCC2 drug substrate, such as irinotecan, in a patient. Such methods and compositions can be used to evaluate whether irinotecan-based therapy, or therapy involving other ABCC2 substrates, may pose toxicity problems if given to a particular patient or predicting their efficacy. Alterations in suggested therapy may ensue based on genotyping results. | 01-15-2009 |
| 20090017453 | NICKING AND EXTENSION AMPLIFICATION REACTION FOR THE EXPONENTIAL AMPLIFICATION OF NUCLEIC ACIDS - The invention is in general directed to the rapid exponential amplification of short DNA or RNA sequences at a constant temperature. | 01-15-2009 |
| 20090017454 | BIOSENSORS, METHOD FOR OBTAINING THE SAME AND USES THEREOF - The invention relates to biosensors, methods for obtaining them and their use for detecting, assaying or locating, in direct immunofluorescence, a ligand such as an antigen or hapten, in a heterogeneous population. The biosensor includes (i) at least one fragment of a receptor which is protein in nature, capable of binding to a ligand via an active site, where at least one amino acid residues of the fragment located in the proximity of the active site is naturally present in the form of a cystein (Cys) residue, or is substituted with a Cys residue, and (ii) a fluorophore coupled to the Cys residue. | 01-15-2009 |
| 20090017455 | Methods and systems for detecting and/or sorting targets - Provided herein are methods and systems for detecting and/or sorting targets in a sample based on the combined use of polynucleotide-encoded-protein and substrate polynucleotides. The polynucleotide-encoded protein is comprised of a protein that specifically binds to a predetermined target and of an encoding polynucleotide that specifically binds to a substrate polynucleotide, wherein the substrate polynucleotide is attached to a substrate. | 01-15-2009 |
| 20090017456 | DETECTION, IDENTIFICATION AND DIFFERENTIATION OF EUBACTERIAL TAXA USING A HYBRIDIZATION ASSAY - The present invention relates to a method for the specific detection and/or identification of | 01-15-2009 |
| 20090017457 | IDENTIFICATION OF MODULATORS OF SERINE PROTEASE INHIBITOR KAZAL AND THEIR USE AS ANTI-CANCER AND ANTI-VIRAL AGENTS - This invention describes a relevant etiology of cancer and a novel anti-cancer therapeutic strategy, based on the discovery that a protein named serine protease inhibitor (SPIK/SPINK/PSTI) was up-regulated by hepatitis B and C virus infections consequently suppressing the cell apoptosis. Accordingly, this invention provides an inhibitor of SPIK and/or a technology of suppression of over-expression of SPIK in cells. The inhibitors include: 1) chemical compounds, which can inhibit SPIK transcripts, protein activity, and gene expression, 2) SPIK siRNA (RNAi gene silence or dsRNA of SPIK, 3) DNA anti-sense and anti-SPIK antibody. Further, this invention provides a method of using the inhibitor as an anti-cancer agent to re-instate cancer cell apoptosis (e.g., serine protease dependent cell apoptosis). | 01-15-2009 |
| 20090017458 | DISEASE DIAGNOSIS APPARATUS AND METHOD USING PHOTOCURRENT OF AMORPHOUS SILICON - Provided is a disease diagnosis apparatus and method for detecting a specific disease. The disease diagnosis chip, includes: a light sensing layer; a probe molecule fixed on the light sensing layer; and an electrode connected to the light sensing layer to detect an electric signal corresponding to photoelectric charges of the light sensing layer. | 01-15-2009 |
| 20090017459 | MEASURING ANALYTES USING COMPETITIVE INTERFERENCE AND RECUPERATION OF ENZYME ACTIVITY - The present teaching generally discloses a method of measuring the concentration of an analyte in a solution. The method includes selecting a binding compound; selecting an analog to an analyte and conjugating the analog to a polynucleotide substrate to construct a reagent for measuring the amount of the analyte in an assay solution using a desired polynucleotide reaction that comprises a polymerization reaction, a cleavage reaction, or a recombination reaction. The binding of the reagent to the binding compound in the assay solution competes with the binding of the analyte to the binding compound in the assay solution. The method also includes selecting a desired enzyme for catalyzing the desired polynucleotide reaction. Reagents and kits are also provided. | 01-15-2009 |
| 20090017460 | DIFFERENTIAL EXPRESSION PROFILING ANALYSIS OF CELL CULTURE PHENOTYPES AND USES THEREOF - The present invention provides, among other things, systems and methods for identifying genes and proteins that regulate and/or are indicative of cell phenotypes based on expression profiling analysis. The present invention further provides methods of manipulating identified genes and proteins to engineer improved cell lines. | 01-15-2009 |
| 20090017461 | Diagnosis of Gynecological Neoplasms by Detecting the Levels of Oviduct-Specific Glycoprotein - The present invention provides a method for detecting cancer in a patient. A sample from the patient is provided, and the level of oviduct-specific glycoprotein (OGP) in the sample is determined and compared to a control sample. Increased levels of OGP in the sample as compared to the control indicates that the patient has cancer. In one aspect, the cancer is a gynecological cancer, such as ovarian cancer. Kits for conducting the methods of the invention are also provided. | 01-15-2009 |
| 20090017462 | Recombinase polymerase amplification - This disclosure describe three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods may allow amplification of DNA up to hundreds of megabases in length. | 01-15-2009 |
| 20090017463 | METHODS FOR PREDICTING PROSTATE CANCER RECURRENCE - The present invention relates to the identification of four cytokine biomarkers in prostatic tissue that exhibit differential expression following prostatectomy that, in combination at least one other factor, are able to reliably predict the development of biochemical recurrence following surgery. This marker combination improves the risk stratification of patients after primary local treatment for localized prostate cancer. | 01-15-2009 |
| 20090017464 | METHODS FOR DRUG DISCOVERY, DISEASE TREATMENT, AND DIAGNOSIS USING METABOLOMICS - The small molecule profiles of cells are compared to identify small molecules which are modulated in altered states. Cellular small molecule libraries, methods of identifying tissue sources, methods for treating genetic and non-genetic diseases, and methods for predicting the efficacy of drugs are also discussed. | 01-15-2009 |
| 20090017465 | Compound Screening Using Cardiomyocytes - This invention provides populations human cells of the cardiomyocyte lineage. The cells are obtained by causing cultures of pluripotent stem cells to differentiate in vitro, and then harvesting cells with certain phenotypic features. Differentiated cells bear cell surface and morphologic markers characteristic of cardiomyocytes, and a proportion of them undergo spontaneous periodic contraction. Highly enriched populations of cardiomyocytes and their replicating precursors can be obtained, suitable for use in a variety of applications, such as drug screening and therapy for cardiac disease. | 01-15-2009 |
| 20090023134 | Neoplasia diagnostic compositions and methods of use - The invention generally features compositions and methods for the diagnosis and monitoring of a neoplasia (e.g., a prostatic neoplasia) in a subject, as well as methods of treatment selection. | 01-22-2009 |
| 20090023135 | DETECTION OF ENHANCED MULTIPLEX SIGNALS BY SURFACE ENHANCED RAMAN SPECTROSCOPY - Various methods of using Raman-active or SERS-active probe constructs to detect analytes in biological samples, such as the nucleic acid and/or protein-containing analytes in a body fluid are provided. | 01-22-2009 |
| 20090023136 | HSD3B1 sequence variants - Isolated HSD3B1 nucleic acid molecules that include a nucleotide sequence variant and nucleotides flanking the sequence variant are described, as are HSD3B1 allozymes. Methods for determining whether a subject contains an HSD3B1 sequence variant also are provided. | 01-22-2009 |
| 20090023137 | ESR1 and Cervical Cancer - The present invention provides methods and kits for detecting susceptibility to, or the incidence of, cervical cancer in a sample comprising determining the methylation status of the ESR1 gene, optionally as part of a panel of genes. Also provided are methods and kits which involve determining the expression levels of genes including the ESR1 gene in order to diagnose cervical cancer. | 01-22-2009 |
| 20090023138 | ORAL CANCER MARKERS AND THEIR DETECTION - Methods of detecting progression from precancer to cancer are provided utilizing toluidine blue staining as well as detecting allelic variation at microsatellite loci. An allelic variation in one or more locus is indicative of a progression from precancer to cancer. | 01-22-2009 |
| 20090023139 | Method For Site-Specifically Introducing Non-Natural Amino Acid Into Protien Using Mitochondrial Protein and Method For Effectively Preparing Trna - This invention is intended to provide a protein synthesis system used for producing a tryptophan analogue-containing non-natural-amino-acid-containing protein that satisfies the following conditions: (i) tRNA that transfers a non-natural amino acid is not recognized by an endogenous aminoacyl tRNA synthetase (aaRS); (ii) it is recognized selectively by aaRS exclusive for a non-natural amino acid; and (iii) endogenous tRNA is not recognized by aaRS exclusive for a non-natural amino acid. In the eukaryotic organism-derived cell-free protein synthesis system, a yeast mitochondrial tryptophanyl tRNA synthetase is used in combination with mitochondrial tRNA | 01-22-2009 |
| 20090023140 | CROSSLINKING AGENT, CROSSLINKING METHOD, METHOD OF CONTROLLING GENE EXPRESSION, AND METHOD OF EXAMINING GENE FUNCTION - The present invention provides a crosslinking agent which have photodegradable protective groups at two ends to crosslink double-stranded nucleic acid, a nucleic acid and a protein or a polypeptide, or proteins or polypeptides, in particular, double-stranded RNA; a method for crosslinking a double-stranded RNA or the like using the same; a method for regulating gene expression, which can control the expression of a target gene at an arbitrary timing and location; and a method for examining a gene function. | 01-22-2009 |
| 20090023141 | METHOD FOR DETECTING BACTERIA OF THE GENUS MYCOBACTERIUM (ACID-FAST BACTERIA) AND KIT FOR THE SAME - An object of the present invention is to provide an oligonucleotide for rapidly and conveniently detecting bacteria of the genus | 01-22-2009 |
| 20090023142 | Methods for Detecting Minimum Residual Disease - The present invention features methods and compositions for identifying markers of minimum residual disease (MRD), as well as markers of metastatic cells. The present invention further provides methods for detecting MRD and metastatic cell in a subject. | 01-22-2009 |
| 20090023143 | Predicting agent for metastasis - By disclosing part of metastasis mechanisms in cancer cells, the metastatic potential of cancer cells will be determinable. Anti-galectin-9 Ab against galectin-9 is contained as an effective component with or without others, thereby enabling the evaluation or detection of galectin-9 expression levels in cancer cells wherein the metastatic potential of the cancer cells can be assessed or detected. | 01-22-2009 |
| 20090023144 | METHOD AND ITS KIT FOR QUANTITATIVELY DETECTING SPECIFIC ANALYTE WITH SINGLE CAPTURING AGENT - The invention provides a method and its kit for quantitatively detecting a specific analyte with a single capturing agent. The quantitative detection of a specific analyte with a single capturing agent comprises: firstly combining the tested analyte with a solid phase capturing agent, then labeling analyte which has been trapped by the capturing agent with a report molecule; secondly eluting the labeled analyte from the complex, recombining the tested analyte with a new solid phase capturing agent, and ascertaining the content of analyte by detecting the report molecule's label signal. The kit of the invention comprises a capturing device, a detecting device, a report molecule for labeling and an analysis substance eluate. The advantages of the invention are the need of one single capturing agent, the capability of detecting for many analytes which can't be tested at present, wide application, high sensibility and low noise. The invention can be applied to diagnosis, medical expertise, new medicine development, application of protein micro array and chip, and fundamental research. | 01-22-2009 |
| 20090023145 | Methods of diagnosing or prognosing Alzheimer's disease - A method for diagnosing or prognosing Alzheimer's disease in a subject, or determining whether a subject is at increased risk of developing Alzheimer's disease, comprising:
| 01-22-2009 |
| 20090023146 | DEVICE FOR PROCESSING AN ANALYTE AND A METHOD OF PROCESSING AND/OR DETECTING AN ANALYTE USING SAID DEVICE - The present invention relates to a device for processing an analyte and to a method of processing and/or detecting an analyte using said device. | 01-22-2009 |
| 20090023147 | DIAGNOSTICS AND THERAPEUTICS FOR OSTEOPOROSIS - Diagnostics and therapeutics for osteoporosis, which are bases upon the identification of a subjects IL-1 haplotype and genotype pattern are described. | 01-22-2009 |
| 20090023148 | Sensors for biomolecular detection and cell classification - A sensor device is provided for detecting an analyte in a sample in which an analyte is bound to a detection reagent to form a bound complex. The device comprises (a) a sample ( | 01-22-2009 |
| 20090023149 | Methods, kits and devices for identifying biomarkers of treatment response and use thereof to predict treatment efficacy - The present invention features methods, kits, and devices for predicting the sensitivity of a patient to a compound or medical treatment. The invention also features methods for identifying gene biomarkers whose expression correlates to treatment sensitivity or resistance within a patient population or subpopulation. | 01-22-2009 |
| 20090023150 | DNA Diagnostics Based on Mass Spectrometry - Fast and highly accurate mass spectrometry-based processes for detecting a particular nucleic acid sequence in a biological sample are provided. Depending on the sequence to be detected, the processes can be used, for example, to diagnose a genetic disease or chromosomal abnormality; a predisposition to a disease or condition, infection by a pathogenic organism, or for determining identity or heredity. | 01-22-2009 |
| 20090023151 | Method For The Labeling And Detection Of Small Polynucleotides - Methods and kits for use in isolating, labeling or detecting a small polynucleotide of interest from a sample. The method entails hybridizing the small polynucleotide to a capture probe, lengthening the small polynucleotide by primer extension and/or ligation, and degrading the capture probe to provide a single stranded extension product. The kits include the capture probe and additional reagents for the extension, ligation and/or degradation reactions. | 01-22-2009 |
| 20090023152 | Transcription factor MHC class II genes, substances capable of inhibiting this transcription factor and medical uses of these substances - The present invention relates to a transcription factor of MHC class II genes and its derivatives, inhibitors down-regulating the expression of MHC class II molecules, process to identify these inhibitors and medical uses of these inhibitors. | 01-22-2009 |
| 20090023153 | Targeted modification of chromatin structure - Methods and compositions for targeted modification of chromatin structure, within a region of interest in cellular chromatin, are provided. Such methods and compositions are useful for facilitating processes such as, for example, transcription and recombination, that require access of exogenous molecules to chromosomal DNA sequences. | 01-22-2009 |
| 20090023154 | RELATING TO MARKING - The invention provides a marking system, markers and methods of use of such marking systems and markers which enable unique marking of articles and subsequent detection of that marking. In particular the invention provides a marking system, the marking system comprising a plurality of different DNA fragment types, each of the plurality of different DNA fragment types comprising a plurality of different length DNA fragments and a method in which a sample of the DNA fragment type is taken, amplified and analysed to determine the identity of the marker for an article. | 01-22-2009 |
| 20090029353 | MOLECULAR DETECTOR - Described herein are embodiments of a method and device comprising an electronic charge detector capable of detecting the presence of small quantities of electric charge generated through a universal signal generation process that is configured to detect any biological agents and biomolecular targets of interest. Electric charge results from signal molecules binding to affinity molecules that are attached to a detection surface. Electronic circuits are configured to detect the induced electric charges on the detection surface. Additional electronic devices then process useful quantitative data regarding specific biomolecular interaction events. | 01-29-2009 |
| 20090029354 | Method on clinical applications in head neck cancer by using DSG3 molecule for predicting malignant degree of cancer, serving as a molecular target and using RNA jamming sequence on inhibition-specific of DSG3 expression - The present invention provide a method for analyzing the DSG3 overexpression in tumor tissues with clinical features of cancer cells to validate that DSG3 overexpression is relates to size, depth and migration of tumor. Therefore, DSG3 overexpression is capable for using in clinical applications, determining malignant degree of tumor, serving as molecular target in Head Neck Cancer (HNC). Moreover, a jamming sequence, RNA, is designed to act on DSG3 mRNA and is effective inhibition-specific DSG3 expression, and then inhibits cell growth, invasion and migration in HNC. | 01-29-2009 |
| 20090029355 | Abnormalities of Phosphatase 2A (PP2A) for Diagnosis and Treatment of Alzheimer's Disease - This invention relates to methods of diagnosing Alzheimer's disease and methods of screening for compounds for the treatment or prevention of Alzheimer's disease. The methods are based upon newly discovered differences in protein phosphatase 2A (PP2A) function and related molecular events in Alzheimer's disease cells compared to control cells. In one embodiment, differences in basal PP2A gene expression in Alzheimer's cells are compared to controls. In another embodiment differences in PP2A protein and enzyme activity are compared in test and control cells. In another embodiment differences in response to substances that inhibit PP2A function are compared. Still another embodiment detects differences in the subcellular distribution of phosphorylated Erk1/2, a substrate of PP2A, in normal and Alzheimer's disease cells. The detection of Alzheimer's disease-specific differences in PP2A function and related events in peripheral tissues provides the basis for highly practical and efficient tests and diagnostic test kits for the early diagnosis of Alzheimer's disease, as well as providing a biochemical basis for identifying therapeutic targets for drug development. | 01-29-2009 |
| 20090029356 | Corynebacterium glutamicum genes encoding novel proteins - Isolated nucleic acid molecules, designated MCP nucleic acid molecules, which encode novel MCP proteins from | 01-29-2009 |
| 20090029357 | Method for diagnosing cancer using cancer-associated deletion gene marker - It is an object of the present invention to provide a novel method for diagnosing cancer by discovering a cancer-associated gene which could not be detected by a conventional technique, and detecting deletion, mutation, or an abnormal expression level of such cancer-associated gene. The present invention provides a method for diagnosing cancer which comprises a step of detecting deletion of the GMDS, ANKRD15, TEK, or EBI2 gene in a test sample by using DNA containing all or part of said gene. | 01-29-2009 |
| 20090029358 | Methods and Compositions for the Diagnosis and Treatment of Schizophrenia - Compositions and methods relating to the diagnosis and treatment of neuropsychiatric disorders, such as schizophrenia, schizoaffective disorders, and bipolar disorders are disclosed. Also provided are methods for screening therapeutic agents having efficacy for the treatment of such disorders. | 01-29-2009 |
| 20090029359 | DIAGNOSTIC METHODS FOR EARLY CANCER DETECTION - The present disclosure is directed to compositions and methods for detecting signs of telomere dysfunction as diagnostic indicators of metastatic disease. More particularly, diagnostic reagents and procedures are provided for analyzing samples to detect elevated expression of TRK2 protein or detect the presence of telomere fusions as an early diagnostic test for cancerous or pre-cancerous cells. In one embodiment the methods of the present disclosure are used to diagnose the existence of, or assess the risk of, breast cancer in an individual. | 01-29-2009 |
| 20090029360 | Nucleic Acid Amplification and Detection of Mycobacterium Species - Oligonucleotides used to prime in vitro nucleic acid amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species within the genus | 01-29-2009 |
| 20090029361 | GROWTH DIFFERENTIATION FACTOR-7 - The present invention provides methods of using polynucleotides that hybridize specifically with a polynucleotide encoding GDF-7 to identify GDF-7 in a sample. | 01-29-2009 |
| 20090029362 | GENE COPY NUMBER PROFILING - The invention relates to copy number profile analysis. Specifically, copy number profile analysis is used to determine whether two or more separate tumors in a single individual are derived from a common source. | 01-29-2009 |
| 20090029363 | METHOD OF SELECTION BY TWO-DIMENSIONAL SEPARATION, OF NUCLEIC ACIDS THAT BIND TO A TARGET WITH HIGH AFFINITY - The invention relates to a method of selection, by two-dimensional separation, of nucleic acids that bind to a target molecule with high affinity from a mixture of nucleic acids, including the following steps: a) subjecting the mixture of nucleic acids to a physico-chemical separation step, thereby obtaining a set of mixed fractions containing the nucleic acids, a run parameter window being associated with every mixed fraction containing the nucleic acids, b) contacting a mixed fraction containing the nucleic acids with the target molecule, thereby obtaining a binding mixture containing nucleic acid/target molecule complexes, c) subjecting the binding mixture from step b) to the same physico-chemical separation step as in step a), thereby selecting nucleic acid/target molecule complexes whose run parameters are outside of the run parameter window. | 01-29-2009 |
| 20090029364 | Specific multiplex analysis of nucleic acids - A method of detecting the presence or absence of variant nucleotides within target nucleic acid sequences, said method comprising the use of at least one pair of target-specific amplification primers capable of hybridizing to target nucleic acid sequences, and the use of at least one set of diagnostic primers, each set consisting of at least two types of diagnostic primers capable of hybridizing to corresponding target nucleic acid sequences 3′-relative to said amplification primers, such that each set of diagnostic primers is semi-nested relative to the corresponding pair of amplification primers. Additionally, the use of said method for genotyping, for diagnostic screening, for assaying the cytosine methylation status and for quantification of nucleic acids is disclosed. | 01-29-2009 |
| 20090029365 | NOVEL GENES, COMPOSITIONS, KITS, AND METHODS FOR IDENTIFICATION, ASSESSMENT, PREVENTION, AND THERAPY OF COLON CANCER - The invention relates to newly discovered nucleic acid molecules and proteins associated with colon cancer. Compositions, kits, and methods for detecting, characterizing, preventing, and treating human colon cancers are provided. | 01-29-2009 |
| 20090029366 | METHODS OF IDENTIFYING MODULATORS OF HYPERPOLARIZATION-ACTIVATED CYCLIC NUCLEOTIDE-GATED (HCN) CHANNELS - Methods, including high-throughput methods, for identifying modulators of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. | 01-29-2009 |
| 20090029367 | POLYMORPHISMS IN PON1 ARE ASSOCIATED WITH ELEVATED ALANINE AMINOTRANSFERASE LEVELS AFTER XIMELAGATRAN OR TACRINE ADMINISTRATION - This invention relates to a method for administering a pharmaceutically useful anticoagulant drug to certain suitable patients and a method for identifying those patients suitable for receiving the drug. In particular, the invention surrounds the identification of an association between certain SNPs in the PON1 gene and susceptibility to increased levels of alanine aminotransferase (ALAT) following ximelagatran or tacrine administration. Thus, this invention relates to methods for predicting susceptibility to elevated ALAT following ximelagatran or tacrine administration and to methods for administering a pharmaceutically useful anticoagulant drug to certain suitable patients. | 01-29-2009 |
| 20090029368 | Method for determining the cell culture history of a cell unit labelled with more than one type of tag - The present invention relates in one aspect to a method for determining the cell culture history of a cell unit labelled with more than one type of tag comprising the steps of: (a) measuring one or more parameters of each tag that is used to label the cell unit; (b) identifying each tag in the cell unit; and (c) correlating the identity of each tag to the identity of the cell unit and/or the specific cell culture conditions to which the cell unit has been exposed. | 01-29-2009 |
| 20090029369 | GENETIC SELECTION SYSTEM TO IDENTIFY PROTEASES, PROTEASE SUBSTRATES AND PROTEASE INHIBITORS - The present invention concerns a tester protein for identifying and/or monitoring protease activity in a cellular assay suitable for high throughput screenings by growth selection, wherein the tester polypeptide is a non-regulatory protein carrying a protease cleavage sequence. Upon co-expression of the protease recognizing said cleavage sequence the tester protein is inactivated, which influences the growth and/or survival of the host cells under the chosen conditions. However, in the presence of protease inhibitor the growth phenotype is reversed. The system can be used to identify proteases, protease inhibitors, and protease cleavage sites. | 01-29-2009 |
| 20090029370 | REAL TIME NUCLEIC ACID DETECTION IN VIVO USING PROTEIN COMPLEMENTATION - The present invention relates to a method to detect nucleic acid molecules, such as RNA molecules in vivo using real time protein complementation methods. The invention further relates to methods for detecting nucleic acids, for example RNA in real-time in living cells with a high sensitivity, using a novel split biomolecular conjugate of the invention. | 01-29-2009 |
| 20090029371 | METHOD FOR DETERMINING VASOREACTIVITY - The present invention is generally directed to methods and materials for determining the genotype of a patient to predict the patient's vasoreactivity. More particularly, the present invention is directed to a method of determining the vasoreactivity of a subject, comprising: obtaining from a subject a sample comprising a nucleic acid sequence of the BMPR2 gene or amino acid sequence of the BMPR2 gene; determining the presence or absence of a non-synonymous mutation in the BMPR2 nucleic acid or amino acid sequence, and correlating the presence of a non-synonymous mutation with non-vasoreactivity or the absence of a non-synonymous mutation with vasoreactivity. | 01-29-2009 |
| 20090029372 | ADAM12 AS A BIOMARKER FOR BLADDER CANCER - The present inventors have shown that the gene and protein expression profiles of ADAM8, ADAM10 and ADAM12 in different grades and stages of bladder cancer. | 01-29-2009 |
| 20090029373 | METHOD FOR THE QUANTITATIVE DETECTION OF NUCLEIC ACIDS - Provided herein is a method for the quantitative detection of HHV-6 subtypes A and/or B based on the use of a calibrator, suitable primers and probes, and a nucleic acid polymerase with 5′-3′ nuclease activity. | 01-29-2009 |
| 20090029374 | SYSTEM AND METHOD FOR IDENTIFYING NETWORKS OF TERNARY RELATIONSHIPS IN COMPLEX DATA SYSTEMS - A system and method for identifying high order associations between variables in complex systems that is particularly useful where there is no correlation or weak correlation between variables due to the influence of a third variable, a ternary relationship. The ternary relationship describes how the variation in the pattern of association between a pair of variables, including its sign and strength, is mediated by a third variable. In one embodiment applied to gene expression data, the activity of pairs of correlated genes due to the activity of one or more third genes is shown. | 01-29-2009 |
| 20090029375 | GENETIC MODELS FOR STRATIFICATION OF CANCER RISK - The present invention provides new methods for the assessment of cancer risk in the general population. These methods utilize particular alleles of in multiple selected genes to identify individuals with increased or decreased risk of breast cancer. In addition, personal history measures such as age and family history are used to further refine the analysis. Using such methods, it is possible to reallocate healthcare costs in cancer screening to patient subpopulations at increased cancer risk. It also permits identification of candidates for cancer prophylactic treatment. | 01-29-2009 |
| 20090029376 | High Fidelity Restriction Endonucleases - Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions include restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. | 01-29-2009 |
| 20090029377 | DIAGNOSING FETAL CHROMOSOMAL ANEUPLOIDY USING MASSIVELY PARALLEL GENOMIC SEQUENCING - Embodiments of this invention provide methods, systems, and apparatus for determining whether a fetal chromosomal aneuploidy exists from a biological sample obtained from a pregnant female. Nucleic acid molecules of the biological sample are sequenced, such that a fraction of the genome is sequenced. Respective amounts of a clinically-relevant chromosome and of background chromosomes are determined from results of the sequencing. A parameter derived from these amounts (e.g. a ratio) is compared to one or more cutoff values, thereby determining a classification of whether a fetal chromosomal aneuploidy exists. | 01-29-2009 |
| 20090029378 | High sensitivity multiparameter method for rare event analysis in a biological sample - A sample of blood containing CTCs, or other cells of interest, is stained with fluorescent markers for image analysis and scanned to identify the presence and location within the cartridge of target cells or subcellular elements. A sample containing desired target cells or subcellular elements is then further processed, in part by photobleaching the sample, so that those same targets may be re-analyzed with additional biomarkers conjugated to the same or different fluorochromes using the same imaging criteria that were used for the initial analysis. The present invention has applications with targets such as circulating epithelial, cells, circulating tumor cells, circulating endothelial cells, leukocytes, lymphocyte subsets, cells containing an organelle or receptor of interest, cellular debris, disrupted cells and their debris, or any other formed element that might be captured and imaged. The invention provides a means to further interrogate individual targets of interest, especially when coupled with genetic analysis such as FISH. | 01-29-2009 |
| 20090029379 | GENETIC MARKERS FOR PROGNOSIS OF ANTIFOLATE TREATMENT EFFICACY - Methods and kits for predicting the efficacy of antifolate (e.g., methotrexate) treatment of rheumatoid arthritis by detecting polymorphisms, particularly single nucleotide polymorphisms, in adenosine pathway genes. | 01-29-2009 |
| 20090029380 | HUMAN MutY - A human mutY polypeptide and DNA (RNA) encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptide for preventing and/or treating diseases associated with a mutation in this gene. Diagnostic assays for identifying mutations in nucleic acid sequence encoding a polypeptide of the present invention and for detecting altered levels of the polypeptide of the present invention for detecting diseases, for example, cancer, are also disclosed. | 01-29-2009 |
| 20090029381 | METHODS AND COMPOSITIONS FOR ANALYZING AHASL GENES - The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of | 01-29-2009 |
| 20090029382 | CHARACTERIZATION OF A MEMBRANE ESTROGEN RECEPTOR - The present invention discloses the identification of a novel membrane associated estrogen receptor, termed mER. The membrane associated receptor is involved in rapid signal transduction. Amino acid sequences, nucleic acid sequences, vectors, and host cells are also discussed. Additionally, methods of detecting agonists and antagonists for the receptor are disclosed herein. | 01-29-2009 |
| 20090029383 | Polynucleotide Sequencing Method - The subject invention pertains to a method for determining the sequence of a polynucleotide comprising the steps of (i) contacting a polynucleotide processive enzyme immobilised in a fixed position, with a target polynucleotide under conditions sufficient to induce enzyme activity; (ii) detecting an effect consequent on the interaction of the enzyme and polynucleotide, wherein the effect is detected by measurement of a non-linear optical signal or a linear signal coupled to a non-linear signal. | 01-29-2009 |
| 20090029384 | TECHNIQUES FOR RECORDING SIGNALS - The present invention provides techniques of recording signals. In one aspect of the invention, a method of recording a signal comprises the following steps. One or more errors are selectively introduced during synthesis of a polymer in response to the signal. The one or more occurrences of the one or more errors in the synthesized polymer are recorded. The synthesis of the polymer may comprise a polymer synthetase that can selectively introduce the one or more errors in response to the signal. A method for analyzing signals is also provided. | 01-29-2009 |
| 20090029385 | Molecular redundant sequencing - Methods, systems and compositions where a target nucleic acid includes a registration sequence disposed therein for identification of the number or relative position of determined sequence from the template sequence. Particularly preferred aspects include a registration sequence in a circular template nucleic acid sequence which is, in turn, used in sequence by incorporation processes that rely upon template dependent, polymerase mediated primer extension in the identification of the sequence of the template. | 01-29-2009 |
| 20090029386 | KIT FOR ENUMERATING MAMMALIAN CELL MICRONUCLEI WITH AN EMPHASIS ON DIFFERENTIALLY STAINING MICRONUCLEI AND THE CHROMATIN OF DEAD AND DYING CELLS - The present invention relates a method for the enumeration of mammalian cell micronuclei, while distinguishing micronuclei from the chromatin of dead and dying cells. The method utilizes differential staining of chromatin from dead and dying cells, to distinguish the chromatin from micronuclei and nuclei that can be detected based upon fluorescent emission and light scatter following exposure to an excitatory light source. Counting of micronuclei events relative to the number of nuclei can be used to assess the DNA-damaging potential of a chemical agent, the DNA-damaging potential of a physical agent, the effects of an agent which can modify endogenously-induced DNA damage, and the effects of an agent which can modify exogenously-induced DNA damage. Kits for practicing the invention are also disclosed. | 01-29-2009 |
| 20090035751 | Single Molecule Detection Using Molecular Motors - The present invention provides methods and compositions for highly sensitive nucleic acid detection, down to the single nucleic acid molecule level. In one aspect, the present invention provides methods for detecting a target nucleic acid comprising: (a) providing first and second target-specific nucleic acids, wherein the first and second target-specific nucleic acids each comprise sequences complementary to the target nucleic acid; wherein the first target specific nucleic acid is bound to a first affinity tag and the second target-specific nucleic acid is bound to a second affinity tag, wherein the first affinity tag is capable of binding to a molecular motor, and wherein the second affinity tag is capable of binding to a detection probe; (b) contacting the first and second target-specific nucleic acids to a sample under conditions whereby the first and second target-specific nucleic acids will hybridize to the target nucleic acid if the target nucleic acid is present in the sample, wherein upon hybridization to the target nucleic acid the first and second target-specific nucleic acids are directly adjacent to each other; (c) ligating the first and second target-specific nucleic acids together; (d) binding the molecular motor t˜ the first affinity tag and the detection probe to the second affinity tag; (e) inducing movement of the molecular motor; and (f) detecting movement of the molecular motor through the detection probe, wherein the movement of the molecular motor serves to detect the target nucleic acid in the sample. | 02-05-2009 |
| 20090035752 | Polynucleotide Participating In Rheumatoid Arthritis And Utilization Of The Same - Analysis is made on the DNA methylation of the region (including a promoter region) upstream from the translation initiation point of a rheumatoid arthritis-associated gene DR3 in human genome. As a result, it is found out that an allele-specific methylation occurs in a CpG sequence located about −380 to −180 bp upstream from translation initiation point (ATG) of the gene DR3. It is further found out that the CpG sequences downstream therefrom of the genes DR3 originating in healthy subjects are all in the unmethylated state, while methylated and unmethylated sequences are both observed in the genes DR3 originating in RA patients. | 02-05-2009 |
| 20090035753 | Novel druggable regions in the dengue virus envelope glycoprotein and methods of using the same - The present invention relates to novel druggable regions discovered in dengue virus envelope glycoprotein, or dengue virus E protein, which is a class II viral E protein. The present invention further relates to methods of using the druggable regions to screen potential candidate therapeutics for diseases caused by viruses having class II E proteins, e.g. viral fusion inhibitors. | 02-05-2009 |
| 20090035754 | Methods and compositions for detecting steroids - The present invention provides for methods and systems for detecting steroids. Examples of such steroids include estrogen, progesterone, androgen, testosterone, and derivatives and analogs thereof. Systems useful for carrying out the method include tripartite constructs including a DNA-binding domain, a ligand binding domain, and an activation domain. The present invention provides numerous improvements over previous diagnostic systems for detection of steroids, such advantages include that the method allows for detection of steroid analogs and derivatives, whose structures may not yet be known, the method is generally applicable to a wide variety of organisms, and numerous ligand binding domains may be used in conjunction with the present system. | 02-05-2009 |
| 20090035755 | Yeast Strain And Screening Method For Identifying Inhibitors Of The Expression Of The Hexose Transporter Genes By A Positive Phenotype - The present invention provides screening procedures for identifying inhibitors of components of regulatory networks by a positive phenotype and modified yeast cell lines suitable for said screening. The screening procedures are especially suited to screen for substances that re-sensitize resistant pathogenic microorganisms or tumor cells by suspending the expression of resistance-relevant genes. The invention further provides methods for constructing said cell lines and their use in screening systems. | 02-05-2009 |
| 20090035756 | Methods for arbitrary peptide synthesis - Methods, apparatus, systems, computer programs and computing devices related to biologically assembling and/or synthesizing peptides and/or proteins are disclosed. | 02-05-2009 |
| 20090035757 | SUBSTRACTIVE SINGLE LABEL COMPARATIVE HYBRIDIZATION - Provided are methods of determining differences between nucleic acids in a test sample and a reference sample. In certain embodiments the methods are used for detecting and mapping chromosomal or genetic abnormalities associated with various diseases or with predisposition to various diseases, or to detecting the phenomena of large scale copy number variants. In particular, provided are advanced methods of performing array-based comparative hybridization that allow reproducibility between samples and enhanced sensitivity by using the same detectable label for both test sample and reference sample nucleic acids. Invention methods are useful for the detection or diagnosis of particular disease conditions such as cancer, and detecting predisposition to cancer based on detection of chromosomal or genetic abnormalities and gene expression level. Invention methods are also useful for the detection or diagnosis of hereditary genetic disorders or predisposition thereto, especially in prenatal samples. Moreover, invention methods are also useful for the detection or diagnosis of de novo genetic aberrations associated with post-natal developmental abnormalities. | 02-05-2009 |
| 20090035758 | Method of Measurement of Micronucleated Erythrocyte Populations - A method of measuring micronucleated erythrocyte populations is disclosed. The method includes contacting a sample containing erythrocyte populations with a fluorescently labeled antibody specific to immature erythrocytes among erythrocyte populations; adding an aqueous permeation reagent to the sample and incubating the formed permeation sample mixture for an incubation time sufficient to allow the permeation reagent to render cellular membrane of the erythrocyte populations permeable to RNase and dye; adding a RNase reagent to degrade RNA and to inhibit further reaction of permeation reagent; adding a fluorescent nucleic acid dye reagent to stain DNA representing micronuclei in the erythrocyte populations; performing light scatter and fluorescence measurements of the final sample mixture on a flow cytometer; differentiating micronucleated erythrocyte populations from other cell types; and reporting the micronucleated erythrocyte populations of the sample. The method avoids ultracold fixation, centrifugation, and washing cells in sample preparation. | 02-05-2009 |
| 20090035759 | METHOD AND DEVICE FOR DETECTING THE PRESENCE OF A SINGLE TARGET NUCLEIC ACID IN A SAMPLE - A method comprises loading a sample portion into a sample chamber which comprises means for minimizing diffusion of the sample portion, subjecting the sample portion to an amplification step, and determining whether the sample portion contains at least one molecule of a target nucleic acid. If the sample portion contains a single molecule of the target nucleic acid, the sample portion would attain a detectable concentration of the target nucleic acid after a single round of amplification. Also, a microfluidic device comprising a sample portion and a sample chamber comprising means for minimizing diffusion of the sample portion. Also, a microfluidic device comprising a sample chamber and an amplification targeting reagent positioned in the first sample chamber. | 02-05-2009 |
| 20090035760 | CalDAG-GEF1 Gene Mutations Associated with Thrombopathy - The present invention relates to methods and compositions for detecting mutations associated with thrombopathy. In particular, the invention relates to methods and composition for detecting mutations in the CalDAG-GEF1 gene that are associated with thrombopathy. | 02-05-2009 |
| 20090035761 | Method for isolation of DNA, RNA and proteins from formalin-fixed paraffin-embedded tissue specimens - Methods are disclosed for rapid, reliable and simple isolation of RNA from formalin-fixed paraffin-embedded tissue samples. RNA purified in this manner can be used to monitor gene expression levels. The tissue sample can be a tumor or other pathological tissue. | 02-05-2009 |
| 20090035762 | Allele-specific copy number measurement using single nucleotide polymorphism and DNA arrays - Methods and systems for allelic detection and allele-specific copy number are provided herein. The described methods use identification of single nucleotide polymorphism using restriction enzymes and CGH analyses. Microarrays comprising probes designed by the described methods are provided. Also included are methods for identifying SNP sites and copy number in samples obtained from patient populations. | 02-05-2009 |
| 20090035763 | Methods for Detecting Progression of Low Grade Cervical Dysplasia and for Detecting Adenocarcinomas - The invention provides methods for identifying conditions of low grade cervical dysplasia and assessing the progressive potential of individual lesions to develop into high grade cervical dysplasia and cervical squamous cell cancer as well as cervical adenocarcinoma. | 02-05-2009 |
| 20090035764 | Methods and systems for evaluating CGH candidate probe nucleic acid sequences - Methods of evaluating candidate CGH probe nucleic acid sequences are provided. Aspects of the methods include providing a candidate CGH probe nucleic acid sequence for a target sequence of a copy number variation (CNV) of a genome. A proximity score is then determined for the candidate CGH probe nucleic acid sequence and employed to evaluate the sequence. Aspects of the invention further include computer programming and systems that include the same which are configured to evaluate candidate CGH probe nucleic acid sequences using a proximity score. | 02-05-2009 |
| 20090035765 | Polynucleotides and methods for making plants resistant to fungal pathogens - This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen | 02-05-2009 |
| 20090035766 | Methods for Analyzing High Dimension Data for Classifying, Diagnosing, Prognosticating, and/or Predicting Diseases and Other Biological States - A method of diagnosing, predicting, or prognosticating about a disease that includes obtaining experimental data, wherein the experimental data is high dimensional data, filtering the data, reducing the dimensionality of the data through use of one or more methods, training a supervised pattern recognition method, ranking individual data points from the data, wherein the ranking is dependent on the outcome of the supervised pattern recognition method, choosing multiple data points from the data, wherein the choice is based on the relative ranking of the individual data points, and using the multiple data points to determine if an unknown set of experimental data indicates a diseased condition, a predilection for a diseased condition, or a prognosis about a diseased condition. | 02-05-2009 |
| 20090035767 | PRIMER FOR BACTERIUM GENOME AMPLIFICATION REACTION - A primer or a primer set can efficiently amplify the gene sequence of 16S rRNA of a target bacterium out of selected sixty two bacterium species. At least one of the primers having a base sequence selected from sequences No. 1 through No. 6 is employed as bacterium genome amplification reaction primer: | 02-05-2009 |
| 20090035768 | Method of Determining Predisposition to Scoliosis and Uses Thereof - The present invention relates to novel genetic markers associated with scoliosis, risk of developing scoliosis and risk of scoliosis curve progression, and methods and materials for determining whether a human subject has scoliosis, is at risk of developing scoliosis or is at risk of scoliosis curve progression. | 02-05-2009 |
| 20090035769 | Genetic Markers Associated with Scoliosis and Uses Thereof - The present invention relates to novel genetic markers associated with scoliosis, risk of developing scoliosis and risk of scoliosis curve progression, and methods and materials for determining whether a human subject has scoliosis, is at risk of developing scoliosis or is at risk of scoliosis curve progression. | 02-05-2009 |
| 20090035770 | Inline-injection microdevice and microfabricated integrated DNA analysis system using same - Methods and microfluidic circuitry for inline injection of nucleic acids for capillary electrophoresis analysis are provided. According to various embodiments, microfabricated structures including affinity-based capture matrixes inline with separation channels are provided. The affinity-based capture matrixes provide inline sample plug formation and injection into a capillary electrophoresis channel. Also provided are methods and apparatuses for a microbead-based inline injection system for DNA sequencing. | 02-05-2009 |
| 20090035771 | Hepatocellular carcinoma-related genes and polypeptides, and method for detecting hepatocellular carcinomas - Genes up-regulated in hepatocellular carcinomas and polypeptides encoded by these genes are provided. Vectors, transformants and methods for producing the recombinant polypeptides are also provided. Probes and primers of these genes and antibodies against the polypeptides are also provided. The probes, primers and antibodies can be used as reagents for detecting hepatocellular carcinomas. Methods for detecting hepatocellular carcinomas using such detection reagents are further provided. Antisense nucleotide sequences of these genes are also provided and can be used to inhibit growth of hepatocellular carcinomas. | 02-05-2009 |
| 20090035772 | Genetic Markers Associated With Scoliosis And Uses Thereof - The present invention relates to novel genetic markers associated with scoliosis, risk of developing scoliosis and risk of scoliosis curve progression, and methods and materials for determining whether a human subject has scoliosis, is at risk of developing scoliosis or is at risk of scoliosis curve progression. | 02-05-2009 |
| 20090035773 | NUCLEIC ACID SEQUENCES FOR DETECTING GENETIC MARKERS FOR CANCER IN A BIOLOGICAL SAMPLE - Nucleic acid sequences for detecting the presence of nucleic acids, particularly mRNA, encoding human prostate-associated genetic markers encoding prostate-specific antigen (PSA), prostate specific membrane antigen (PSMA) or human kallikrein 2 (hK2) are disclosed. Preferred combinations of nucleic acid sequences amplifying and detecting the prostate-associated genetic markers RNA, used in methods that include amplification of the target sequences and detection of the amplified sequences are disclosed. Methods of detecting the presence of prostate-associated genetic marker nucleic acids, particularly mRNA, in a biological sample of non-prostate origin are disclosed. | 02-05-2009 |
| 20090035774 | METHOD FOR CHARACTERIZING PRIMARY TUMORS - The invention relates to a method for the detection and characterisation of primary tumours and separate areas of primary tumours, respectively. Clusters of tumour cells, extracted from sample material, are isolated and concentrated, followed by an analysis of genetic changes in these isolated cell clusters. | 02-05-2009 |
| 20090035775 | PCR-BASED DETECTION METHOD FOR CHLAMYDIA TREACHOMATIS - A process for designing of PCR-based detection method for | 02-05-2009 |
| 20090035776 | Method and Kit for Hla-B Genotyping Based on Real-Time Pcr - Method and kit based on real-time PCR with specific primers and probes that allow to achieve a high degree of subtyping of the complete HLA-B locus. The main advantages are the greater speed (65 minutes, including the interpretation); the ease of automation, since only eighteen tubes are necessary to obtain a good level of resolution (typing of 300 groups); reduction of the total cost per test thanks to the ease of automation and the simplicity; a surprisingly high degree of allele definition is achieved; and the risk of sample contamination is reduced because the amplified products always remain in the tubes and no post-PCR steps are necessary. | 02-05-2009 |
| 20090035777 | HIGH THROUGHPUT NUCLEIC ACID SEQUENCING BY EXPANSION - Nucleic acid sequencing methods and related products are disclosed. Methods for sequencing a target nucleic acid comprise providing a daughter strand produced by a template-directed synthesis, the daughter strand comprising a plurality of subunits coupled in a sequence corresponding to a contiguous nucleotide sequence of all or a portion of the target nucleic acid, wherein the individual subunits comprise a tether, at least one probe or nucleobase residue, and at least one selectively cleavable bond. The selectively cleavable bond(s) is/are cleaved to yield an Xpandomer of a length longer than the plurality of the subunits of the daughter strand, the Xpandomer comprising the tethers and reporter elements for parsing genetic information in a sequence corresponding to the contiguous nucleotide sequence of all or a portion of the target nucleic acid. Reporter elements of the Xpandomer are then detected. Corresponding products, including Xpandomers and oligomeric and monomeric substrate constructs are also disclosed. | 02-05-2009 |
| 20090035778 | METHODS AND COMPOSITIONS FOR CORRELATING GENETIC MARKERS WITH MULTIPLE SCLEROSIS - The present invention provides, in certain aspects, a method of identifying a subject as having an increased risk of developing multiple sclerosis, comprising detecting in the subject the presence of a nucleotide variant in the interleukin 7 receptor alpha chain gene, whereby the presence of said variant identifies the subject as having an increased risk of developing multiple sclerosis. | 02-05-2009 |
| 20090035779 | SYSTEMS AND METHODS FOR DETERMINING CROSS-TALK COEFFICIENTS IN PCR AND OTHER DATA SETS - Systems and methods for determining cross-talk coefficients in curves, such as sigmoid-type or growth curves, and PCR curves and nucleic acid melting curves in particular, as well as for applying the cross-talk coefficients to produce cross-talk corrected data sets using a linear subtractive model. Cross-talk signal coefficients are determined using cross-talk data acquired across the entire signal acquisition range. Analyzing across all of the signal curve data provides for a more robust cross-talk correction across the entire data acquisition range. A linear subtractive model is used to correct data sets having cross-talk components. | 02-05-2009 |
| 20090035780 | DETECTION OF METHICILLIN-RESISTANT AND METHICILLIN-SENSITIVE STAPHYLOCOCCUS AUREUS IN BIOLOGICAL SAMPLES - Disclosed are methods of identifying a methicillin-resistant | 02-05-2009 |
| 20090035781 | METHODS AND COMPOSITIONS FOR IDENTIFYING A SUBJECT WITH AN INCREASED RISK OF GRAM NEGATIVE BACTERIAL INFECTION - The present invention provides method of identifying a subject as having an increased risk of developing a Gram negative bacterial infection and/or as having an increased risk of mortality, comprising genotyping the subject for the presence of particular alleles of the lipopolysaccharide binding protein gene, wherein the presence of said allele(s) identifies the subject as having an increased risk of developing a Gram negative bacterial infection and/or of having an increased risk of mortality. | 02-05-2009 |
| 20090035782 | BREEDING PLANTS - A process for breeding plants which comprises growing plants of a species in an array of containers charged with growing medium of uniform characteristics in an environment of controlled climatic conditions with controlled supply of nutrients and feed water and changing the positions of the containers within the environment as required to ensure at least substantially uniform exposure of all plants in the containers to conditions in the environment. A process for the breeding of open pollinating plants in a greenhouse environment is also provided. A process for breeding plants which comprises identifying trait leads. | 02-05-2009 |
| 20090042183 | Use of pcr-based techniques to analyze compositions of botanicals - Methods for use in identifying the individual biological components present in a botanical mixture are provided. Using a combination of genomic-locus specific PCR, single strand conformation polymorphism, and sequence analysis, the biologic components of a botanical composition are identified without prior knowledge as to which components may be present. | 02-12-2009 |
| 20090042184 | Method Of Diagnosing Cancer And Reagents Therefor - The present invention provides methods for diagnosis and monitoring the efficacy of treatment of a cancer. More particularly, the methods of the invention comprise detecting an enhanced degree of chromatin modification within Chromosome 2 of the human genome from about map position 2q14.1 to about map position 2q14.3 in a sample derived from a subject. The methods include detecting an enhanced level of methylation, or detecting an enhanced level of modification of a histone positioned within the chromatin within the region of about 2q14.1 to 2q14.3 of Chromosome 2. The methods also include detecting a modulated level of expression of a gene within the region of about 2q14.1 to 2q14.3 of Chromosome 2. The gene may be selected from the group consisting of DEAD box polypeptide 18 (DDX18), translin (TSN), v-ral simian leukaemia viral oncogene homolog B (RALB), secretin recepto (SCTR), engrailed homolog 1 (EN1), macrophage receptor with collagenous structure (MARCO), protein tyrosine phosphatase non-receptor type 4 (PTPN4), insulin induced gene 2 (INSIG2), inhibin beta B (INHBB), GLI-Kruppel family member 2 (GLI2), FLJ10996, STEAP3, diazepam binding inhibitor (DBI), MGC10993, erythrocyte membrane protein band 4.1 like 5 (EPB41L5), FLJ14816, transcription factor CP2-like 1 (TFCP2L1). | 02-12-2009 |
| 20090042185 | Novel polymorphism in bovine prion protein gene sequence - A specific, non-synonymous SNP in the Prnp gene encoding the bovine prion protein affects the susceptibility of bovine animals to bovine spongiform encephalopathy (BSE). Depending on the number of octapeptide repeat units present in the Prnp gene, the position of the SNP is either nucleotide 631 of exon 3 (codon 211) when the Prnp gene comprises six octapeptide repeat region sequences, nucleotide 607 of exon 3 (codon 203) when the Prnp gene comprises five octapeptide repeat region sequences, or nucleotide 655 of exon 3 (codon 219) when the Prnp gene comprises seven octapeptide repeat region sequences. Alleles of the bovine Prnp wherein the SNP at these positions is lysine (K) at the corresponding amino acids (i.e., 211, 203 or 219) in the bovine prion protein are all indicative of increased susceptibility to BSE in comparison to alleles which encode glutamic acid (E) at the same position. This SNP may be used as a marker for selecting bovines susceptible to BSE for disposal and/or removal from breeding, the human food and animal feed supplies. | 02-12-2009 |
| 20090042186 | MAPPING NEW SITES FOR ANTIBIOTIC ACTION IN THE RIBOSOME - The present invention provides a method of mapping and identifying new sites for antibiotic action in the ribosome of a microorganism. The method comprises: (a) providing a random mutant library of the rRNA genes of the microorganism prepared by randomly mutating the rRNA genes of the microorganism; (b) enriching the library in clones with deleterious rRNA mutants by negative selection; (c) screening for clones with deleterious rRNA mutations; (d) mapping the deleterious rRNA mutations in the clones obtained from step (c) to identify sites in the rRNA which are important functional sites in the ribosome; and (e) selecting functional sites identified in the rRNA in step (d) which are not targeted by a known antibiotic as new sites for antibiotic action for the microorganism. The rRNA gene can be the 16S rRNA of the small subunit or the 23S rRNA or the 5S rRNA of the large subunit of the ribosome of the microorganism. | 02-12-2009 |
| 20090042187 | COMPOSITIONS AND METHODS FOR PREDICTING OUTCOME OF TREATMENT - This invention is directed to compositions and their uses for detection of markers. Such markers may be useful in the understanding of the underlying molecular event leading to a condition or a disease in a subject. These markers may also be useful for characterization of neoplastic cells and cancer cells and their response to certain therapeutical regimes. Therefore the invention as disclosed may contribute to the improvement of the stratification of patients for the best possible treatment. | 02-12-2009 |
| 20090042188 | Agent for Differentiating Hematopoietic Stem Cell Into Natural Killer Cell Comprising Vdup1 Protein or Gene Encoding the Same, and a Method of Differentiating Hematopoietic Stem Cell Into Natural Killer Cell Using Thereof - The present invention is related to an agent for differentiating hematopoietic stem cell into natural killer cell comprising VDUP1 protein or gene encoding the same, and a method of differentiating hematopoietic stem cell into natural killer cell using thereof. The present invention reveals for the first time that the VDUP1 gene is a critical factor for the regulation of differentiation of natural killer cell by generating a mouse deficient in VDUP1 gene, which confirms that VDUP1 gene is required for NK maturation. Thus, through the regulation of VDUP1 gene, the modulation of NK cells that have ability to kill cancer cells is possible and can be utilized for cell therapeutics. | 02-12-2009 |
| 20090042189 | Increased sensitivity of nucleic acid-based detection of organisms by fractionation of target genomes - A method of analyzing a sample for detection of presence or absence of an organism of interest in the sample comprising (a) obtaining a sample; (b) subjecting the sample to a disruption treatment sufficient to fractionate any nucleic acid sequences present in the sample; and (c) detecting for presence or absence of an organism of interest in the sample. The detecting step (c) may comprise performing primer-directed amplification (preferably polymerase chain reaction) on the sample to produce an amplification result and analyzing the amplification result for an amplification product, wherein presence or absence of the amplification product is indicative of the presence or absence of the organism of interest in the sample. | 02-12-2009 |
| 20090042190 | Method of detecting gene - A method of detecting a gene including immobilizing a primer for DNA elongation onto an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a phosphorylcholine group and a second unit having a carboxylic acid-derived group having an electron-attractive substituent bound to a carbonyl group; annealing the template DNA fragments or RNA fragments with the primer for DNA elongation, so as to elongate the DNA primer while incorporating therein an enzyme, thereby allowing coloration of a chromogenic reagent by its enzymatic action; and judging whether the DNA fragments or RNA fragments of the gene presents or not, based on the degree of coloration. | 02-12-2009 |
| 20090042191 | SYSTEM AND METHOD FOR SECURE DOCUMENT PRINTING AND DETECTION - A method for authenticating and verifying an item to be genuine is described. The method for authenticating the item comprises applying a particular nucleic acid material associated with a particular sequence of nucleic acid bases to ink within an ink cartridge or a toner compound within a toner housing. The method also comprises collecting a sample of either the ink or toner compound and verifying the ink or toner is genuine by detecting the particular nucleic acid material. | 02-12-2009 |
| 20090042192 | UNIVERSAL AMPLIFICATION OF FRAGMENTED RNA - The invention relates to methods of using fragmented RNA, such as RNA obtained from archived fixed paraffin-embedded tissue material (FPET RNA) or other clinically biopsied tissue specimens for universal gene expression profiling. | 02-12-2009 |
| 20090042193 | Assays for measuring nucleic acid binding proteins and enzyme activities - Processes for measuring DNA or RNA binding proteins, specific nucleic acids, as well as enzyme activities using labeled nucleic acids of labeled protein/peptide molecules are provided. | 02-12-2009 |
| 20090042194 | Predicting a response to risperidone - The invention relates generally to the relative effect of specific genetic polymorphisms in predicting the clinical outcome of risperidone therapy in patients suffering from a psychiatric disease such as schizophrenia. | 02-12-2009 |
| 20090042196 | METHODS FOR IDENTIFYING MULTIPLE DNA ALTERATION MARKERS IN A LARGE BACKGROUND OF WILD-TYPE DNA - Methods for simultaneously surveying the status of a large number of DNA mutation markers are described. In addition, methods for simultaneously determining the methylation status at multiple sites of a collection of genes, in a single assay, are described. | 02-12-2009 |
| 20090042197 | METHOD FOR DETECTING AND AMPLIFYING NUCLEIC ACID - Problem to be Solved There is provided a method for detecting and/or amplifying a nucleic acid contained in a biological sample such as blood or cells conveniently, rapidly, and effectively. | 02-12-2009 |
| 20090042198 | METHOD FOR MEASURING CONCENTRATION OF NUCLEIC ACIDS - A method for measuring a concentration of nucleic acids is described. The method includes providing bioprobe molecules that include single-stranded nucleic acids, wherein the bioprobe molecules are conjugated with magnetic beads in a solution. A sample of single-stranded target nucleic acids is added to solution, where the single-stranded target nucleic acids hybridize with the single-stranded nucleic acids of the bioprobe molecules. A reduction of the ac (alternating current) magnetic susceptibility of the solution prior and after the addition of the sample to the solution is determined. | 02-12-2009 |
| 20090042199 | Methods and Materials Relating to CD84-like Polypeptides and Polynucleotides - The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel human secreted CD84-like polypeptide. These polynucleotides comprise nucleic acid sequences isolated from cDNA library from human spleen (Hyseq clone identification numbers 2938352 (SEQ ID NO: 1)). Other aspects of the invention include vectors containing processes for producing novel human secreted CD84-like polypeptides, and antibodies specific for such polypeptides. | 02-12-2009 |
| 20090042200 | CELLOMICS SYSTEM - In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip. | 02-12-2009 |
| 20090042201 | Biomarkers for multiple sclerosis and methods of use thereof - Disclosed are biomarkers, the expression of which is differentially regulated in subjects with multiple sclerosis (MS) as compared to subjects that do not have MS. Also described are methods of identification of such biomarkers, and methods of using such biomarkers as targets for the development and identification of therapeutic compounds and strategies for the treatment of MS, as well as methods and kits for the diagnosis of MS. | 02-12-2009 |
| 20090042202 | PROSTATE-SPECIFIC POLYPEPTIDE PAMP AND ENCODING NUCLEIC ACID MOLECULES - The present invention relates to novel prostate specific nucleic acid molecules and polypeptides and related methods for diagnosing or predicting susceptibility to a prostate neoplastic condition. | 02-12-2009 |
| 20090042203 | Mass Spectrometric Methods for Detecting Mutations in a Target Nucleic Acid - Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and mutations in the molecules are provided. | 02-12-2009 |
| 20090042204 | ACNE LESION BIOMARKERS AND MODULATORS THEREOF - The present invention relates to acne lesions biomarkers/genes expression products pattern and particularly inflammatory acne lesions biomarkers and their uses, modulators thereof and the use of modulators for acne treatment or associated disorders. Invention also concerns in vitro diagnostic methods. | 02-12-2009 |
| 20090042205 | FLUORESCENCE DETECTION OF DNA BREAKS USING MOLECULAR OSCILLATORS - A method to detect DNA breaks includes providing a mixture of fluorescence energy transfer molecular oscillators and a DNA sample. The FET oscillator is a synthetic oligonucleotide that has a topoisomerase recognition sequence, a fluorescence donor and a fluorescence acceptor. The synthetic oligonucleotide is bound to a type I topoisomerase capable of binding to the topoisomerase recognition sequence. The mixture is irradiated at a wavelength of the fluorescence donor, and the emission is measured. Another variant of the disclosure is a probe for detecting DNA breaks utilizing a synthetic oligonucleotide comprising a topoisomerase recognition sequence, a fluorescence donor, and a nonradiative fluorescence quencher. Yet another variant of the disclosure is a probe for detecting DNA breaks utilizing a synthetic oligonucleotide comprising a topoisomerase recognition sequence, a fluorescence donor, and a fluorescence acceptor. The mixture is irradiated at a wavelength of the fluorescence donor; and the emission is measured. A method to detect DNA breaks may use these probes in a manner similar to that of the FET oscillator. The FET oscillators and probes are capable of being prepared in a kit formulation. | 02-12-2009 |
| 20090042206 | Multiplexed Analyses of Test Samples - The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer). The methods described create a nucleic acid surrogate for a non-nucleic acid target, thus allowing the wide variety of nucleic acid technologies, including amplification, to be applied to a broader range of desired targets, especially protein targets. The disclosure further describes aptamer constructs that facilitate the use of aptamers in a variety of analytical detection applications. | 02-12-2009 |
| 20090042207 | INTRACELLULAR EXPRESSION AND DELIVERY OF siRNAs IN MAMMALIAN CELLS - The present invention relates to compositions and methods for intracellular expression and delivery of siRNAs in mammalian cells. The siRNA is transcribed intracellularly as a double stranded RNA of about 18 to about 25 base pairs long from an expression cassette. Intracellular expression of siRNA is effective at reducing or eliminating expression of the targeted genes, and is applicable to reverse genetic analysis of genes and to genetic therapy, as for example to inhibiting expression of pathogenic genes and oncogenes. | 02-12-2009 |
| 20090047666 | Methods and nucleic acids for the analysis of colon proliferative disorders - The invention provides methods, nucleic acids and kits for detecting colon cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients. | 02-19-2009 |
| 20090047667 | Salivary transcriptome diagnostics - The present invention concerns probes and methods useful in diagnosing, identifying and monitoring the progression of disease states through measurements of gene products in saliva. | 02-19-2009 |
| 20090047668 | Novel method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same - A method for achieving site specific integration of a desired DNA at a target site in a mammalian cell via homologous recombination is described. This method provides for the reproducible selection of cell lines wherein a desired DNA is integrated at a predetermined transcriptionally active site previously marked with a marker plasmid. The method is particularly suitable for the production of mammalian cell lines which secrete mammalian proteins at high levels, in particular immunoglobulins. Novel vectors and vector combinations for use in the subject cloning method are also provided. | 02-19-2009 |
| 20090047669 | DNA recombination junction detection - The present invention provides methods, compositions and kits for detecting the presence or absence of an integrated insertion polynucleotide. | 02-19-2009 |
| 20090047670 | HYBRIDIZATION-BASED BIOSENSOR CONTAINING HAIRPIN PROBES AND USE THEREOF - A sensor chip that includes: a fluorescence quenching surface; a nucleic acid probe that contains first and second ends with the first end bound to the fluorescence quenching surface, and is characterized by being able to self-anneal into a hairpin conformation; and a first fluorophore bound to the second end of the first nucleic acid molecule. When the first nucleic acid molecule is in the hairpin conformation, the fluorescence quenching surface substantially quenches fluorescent emissions by the first fluorophore; and when the first nucleic acid molecule is in a non-hairpin conformation, fluorescent emissions by the fluorophore are substantially free of quenching by the fluorescence quenching surface. Various nucleic acid probes, methods of making the sensor chip, biological sensor devices that contain the sensor chip, and their methods of use are also disclosed. | 02-19-2009 |
| 20090047671 | SPECIFIC AND UNIVERSAL PROBES AND AMPLIFICATION PRIMERS TO RAPIDLY DETECT AND IDENTIFY COMMON BACTERIAL PATHOGENS AND ANTIBIOTIC RESISTANCE GENES FROM CLINICAL SPECIMENS FOR ROUTINE DIAGNOSIS IN MICROBIOLOGY LABORATORIES - The present invention relates to DNA-based methods for universal bacterial detection, for specific detection of the common bacterial pathogens | 02-19-2009 |
| 20090047672 | Telomerase RNA Subunit and Methods of Use Thereof - The present invention provides a novel telomere associated RNA (hTERC-2) that mediates the DNA repair function of telomerase. | 02-19-2009 |
| 20090047673 | Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids - The invention provides miniaturized lateral flow chromatographic and lateral flow chromatographic microarray devices (LFM). The miniaturization of lateral flow nucleic acid detection achieved by the present invention offers reduced reagent use, femtomole sensitivity, excellent linear dynamic range, and rapid detection. Moreover, the small feature sizes of capture oligonucleotides renders the potential information capacity of the platform comparable to more traditional spotted fluorescence microarrays as well as improving sensitivity. The LFM devices exemplified herein enable analytes to be detected within 10 seconds from the time of sample introduction to the LFM device. Sample volumes may be as low as about 10 microliters, significantly reducing assay costs and ameliorating reagent storage logistics. Additionally, the miniaturization of lateral flow opens the door to highly multiplexed assays, allowing many proteins or nucleic acids to be detected in a single assay. | 02-19-2009 |
| 20090047674 | METHOD FOR NUCLEIC ACID ISOLATION AND AMPLIFICATION - The present invention provides methods and compositions for sequence-specific isolation of polynucleotide molecules from nucleic acid populations and subsequent amplification of isolated polynucleotide molecules or fragments thereof. | 02-19-2009 |
| 20090047675 | Compositions and methods for indentifying transforming and tumor suppressor genes - Provided herein are nucleic acids, proteins, vectors, cells, kits, devices and methods useful for identifying regulatable proteins that are able to complement components of cellular signaling pathways. Also provided are compositions and methods using these complementing genes directly as markers for cancer diagnosis or prognosis and as targets for anti-neoplastic therapeutics. Further provided are methods for using changes caused by expression of the complementing genes to indirectly identify associated genes to be used as markers for cancer diagnosis or prognosis and as targets for anti-neoplastic therapeutics. | 02-19-2009 |
| 20090047676 | System and method for obtaining and maintaining high-resistance seals in patch clamp recordings - The invention provides a system, system components, and a method for rapidly obtaining and stably maintaining a cell in optimal contact with the cell-contacting surface of a sensor in a cell-based biosensor. In one aspect, the system maximizes the seal between a whole cell and the cell-contact surface of a patch clamp micropipette, maximizing the efficiency of a whole cell patch clamp recording. | 02-19-2009 |
| 20090047677 | METHODS FOR GENERATING A DISTRIBUTION OF OPTIMAL SOLUTIONS TO NONDETERMINISTIC POLYNOMIAL OPTIMIZATION PROBLEMS - The present invention overcomes problems in prior art DNA-based computing methods for solving non-deterministic polynomial optimization problems, by providing methods that derive the most probable answers in a statistically significant manner that makes the methods scalable with increases in the number of data inputs, and thus makes the methods practical. | 02-19-2009 |
| 20090047678 | ACCELERATED CASCADE AMPLIFICATION (ACA) OF NUCLEIC ACIDS COMPRISING STRAND AND SEQUENCE SPECIFIC DNA NICKING - Particular aspects provide nucleic acid amplification and detection methods comprising: providing a reaction mixture containing a target nucleic acid with an amplifiable target sequence, forward and reverse external nick-directing primers (ND-primers), at least one internal ND-primer, a strand-displacing DNA polymerase, a nick-directing endonuclease for strand-specific cleavage of ND-primer-extension products, and deoxynucleoside 5′-triphosphates; and incubating the reaction mixture with reagents, and under conditions suitable to provide for amplification of the amplifiable target sequence, wherein the amplification comprises primer extension, by least one internal ND-primer, of an external ND-primer extension product comprising the amplifiable target sequence or a portion thereof but lacking the respective external ND-primer sequence or a portion thereof. Preferably, amplification comprises using a plurality of internal ND-primers, extension of one internal ND-primer extension product by a different internal ND-primer, and amplification is isothermal and synergistic with respect to the number of primers employed. Amplification and detection kits are provided. | 02-19-2009 |
| 20090047679 | METHOD AND SYSTEM FOR ANALYZING REACTIONS USING AN INFORMATION SYSTEM - A method and system for determining the quantity of an analyte initially present in a chemical and or biological reaction as well as a computer implemented method and system to automate portions of the analysis comprising mathematical or graphical analysis of an amplification reaction. | 02-19-2009 |
| 20090047680 | METHODS AND COMPOSITIONS FOR HIGH-THROUGHPUT BISULPHITE DNA-SEQUENCING AND UTILITIES - The invention relates to novel methods and compositions to produce DNA templates suitable for chemical modifications and high-throughput DNA-sequencing. A method of the invention relates to a DNA adaptor design where constituent deoxycytosines are substituted with 5-methyl-deoxycytosines rendering the resulting adaptor resistant to bisulphite mediated deamination. When said adaptor is ligated onto double stranded DNA template, subsequent DNA denaturation and bisulphite treatment deaminates template DNA deoxycytosine differentially to deoxyuraeil whilst the 5-methyl-deoxycytosines of the ligated adaptor resist chemical conversion resulting in the adaptor sequence remaining unaltered. Both strands of bisulphite treated DNA can thus be amplified with a single primer set that hybridizes to the unaltered adaptor sequence. The invention also relates to methods to produce control template of a defined methylation composition to optimize conditions for the bisulphite reaction. In a preferred embodiment, the present invention can be used to produce templates suitable for genome-wide bisulphite-DNA sequencing using conventional, Solexa™, SOLiD™ or 454™-type DNA sequencing platforms to study DNA methylation. | 02-19-2009 |
| 20090047681 | ENTROPIC TRAPPING AND SIEVING OF MOLECULES - Nanofluidic entropic traps, comprising alternating thin and thick regions, sieve small molecules such as DNA or protein polymers and other molecules. The thick region is comparable or substantially larger than the molecule to be separated, while the thin region is substantially smaller than the size of the molecules to be separated. Due to the molecular size dependence of the entropic trapping effect, separation of molecules may be achieved. In addition, entropic traps are used to collect, trap and control many molecules in the nanofluidic channel. A fabrication method is disclosed to provide an efficient way to make nanofluidic constrictions in any fluidic devices. | 02-19-2009 |
| 20090047682 | Method of detecting and predicting bronchodilatory response to beta agonist - Present invention relates to a method for predicting an individual's bronchodilatory response to a β agonist. Present invention particularly relates to the detection of specific allelic variants of the β2AR gene and their use as pharmacogenetic markers towards response to β agonist. | 02-19-2009 |
| 20090047683 | CYANINE DYE COMPOUNDS - Cyanine dye compounds having a negatively charged substituent that are nucleic acid stains, particularly for fluorescent staining of DNA, including compounds having the formula | 02-19-2009 |
| 20090047684 | Gene and protein expression profiles associated with the therapeutic efficacy of irinotecan - The present invention includes gene and protein expression profiles indicative of whether a cancer patient is likely to respond to treatment with irinotecan. By identifying such responsiveness, a treatment provider may determine in advance those patients who would benefit from such treatment, as well as identify alternative therapies for non-responders. The present invention further provide methods of using the gene and/or protein expression profiles and assays for identifying the presence of a gene and/or protein expression profile in a patient sample. | 02-19-2009 |
| 20090053693 | Identification of polymorphisms in the epcr gene associated with thrombotic risk - The invention relates to an in vitro method for determining the risk of developing thrombosis in a subject, which method involves identifying a particular haplotype EPCR gene. | 02-26-2009 |
| 20090053694 | Photochemically Amplified Bioassay - In the present invention, a reagent capable of immunospecific reaction with the analyte of interest is conjugated to a photocatalytic microparticle. After the immunospecific binding has occurred, the assay amplification is performed by exposing photocatalytic particles to actinic UV light in presence of an oxidizable compound. Photocatalytic particles are catalyzing multiple occurrences of oxidation of oxidizable compound under UV light irradiation resulting in detectable changes such as color change. This provides for amplification of each single act of immunospecific binding and is followed by colormetric detection. Thus a high sensitivity quantitative or qualitative immunoassay can be realized. | 02-26-2009 |
| 20090053695 | Gene Marker and Use Thereof | 02-26-2009 |
| 20090053696 | Biomarker For Heart Failure - The present invention relates, in general, to heart failure, and, in particular, to a method of evaluating heart failure patients by monitoring β-adrenergic receptor kinase (βARK1) levels in lymphocytes from such patients. | 02-26-2009 |
| 20090053697 | Pharmacological Applications of Mitochondrial DNA Assays - The invention provides assays to determine the relative amount of mitochondrial DNA in a subject, such as a subject undergoing drug treatment. The subject may for example be a human patient undergoing treatment for an HIV infection with a nucleic acid precursor such as a nucleoside or nucleotide analogue. The assays of the invention may include PCR assays, such semi-quantitative or quantitative PCR involving the co-amplification of a mitochondrial sequence and a reference sequence, such as a genomic sequence. Information from such assays may be evaluated to provide a ratio of mithchondrial DNA to nuclear DNA in the cells of the subject. | 02-26-2009 |
| 20090053698 | METHOD FOR OBTAINING SUBTRACTION POLYNUCLEOTIDE - The present invention provides a method for obtaining or amplifying a polynucleotide (a tester-specific polynucleotide), in which an amount existing in a sample (tester) is larger than the amount existing in another sample (driver), easily and within a short time as well as with high efficiency, a polynucleotide obtained (amplified) by such method, a method for identifying gene mutation in the tester, and a kit to be used in such methods. | 02-26-2009 |
| 20090053699 | Method for Preparing Polynucleotides for Analysis - A method for analysing a target polynucleotide having distinct units of nucleic acid sequence comprising: (i) forming a first polynucleotide which is a concatemer having multiple repeating target polynucleotide sequences; (ii) forming on the first polynucleotide a second polynucleotide hybridised to a portion of one or more of the target polynucleotides, such that the portion hybridised, or the portion not hybridised, corresponds to a sequence unit on the target, and determining the sequence unit on the target. | 02-26-2009 |
| 20090053700 | Optical sorting method - The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; (b) expressing the genetic elements to produce their respective gene products within the microcapsules; (c) sorting the genetic elements which produce the gene product having the desired activity using a change in the optical properties of the genetic elements. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention. | 02-26-2009 |
| 20090053701 | Detectable nucleic acid tag - Provided herein are nucleic acid tags that are linked to, or capable of linking to, a protein of interest. In particular, the nucleic acid tags are oligonucleotides comprising a reporter function and a protein tagging function. Also provided herein, are nucleic acid tag compositions, kits and methods of use thereof. | 02-26-2009 |
| 20090053702 | SPECIFIC AND UNIVERSAL PROBES AND AMPLIFICATION PRIMERS TO RAPIDLY DETECT AND IDENTIFY COMMON BACTERIAL PATHOGENS AND ANTIBIOTIC RESISTANCE GENES FROM CLINICAL SPECIMENS FOR ROUTINE DIAGNOSIS IN MICROBIOLOGY LABORATORIES - The present invention relates to DNA-based methods for universal bacterial detection, for specific detection of the common bacterial pathogens | 02-26-2009 |
| 20090053703 | SPECIFIC AND UNIVERSAL PROBES AND AMPLIFICATION PRIMERS TO RAPIDLY DETECT AND IDENTIFY COMMON BACTERIAL PATHOGENS AND ANTIBIOTIC RESISTANCE GENES FROM CLINICAL SPECIMENS FOR ROUTINE DIAGNOSIS IN MICROBIOLOGY LABORATORIES - The present invention relates to DNA-based methods for universal bacterial detection, for specific detection of the common bacterial pathogens | 02-26-2009 |
| 20090053704 | STABILIZATION OF NUCLEIC ACIDS ON SOLID SUPPORTS - The present invention provides methods, compositions, and kits for the storage and stabilization of biological molecules. The methods comprise applying Tris(2-carboxyethyl)phosphine (TCEP) to at least one biological molecule bound to a solid substrate and storing in an organic solvent. Preferably, the biological molecules are nucleic acids. Compositions and kits for performing the process according to the invention are also provided. | 02-26-2009 |
| 20090053705 | METHODS FOR INCREASING ACCURACY OF NUCLEIC ACID SEQUENCING - The invention provides methods for improving the accuracy of a sequencing-by-synthesis reaction by sequencing at least a portion of a template and at least a portion of template complementary sequence. | 02-26-2009 |
| 20090053706 | DNA METHYLATION MARKERS ASSOCIATED WITH THE CPG ISLAND METHYLATOR PHENOTYPE (CIMP) IN HUMAN COLORECTAL CANCER - Particular aspects confirm the existence of a CpG island methylator phenotype (CIMP) in colorectal cancer, and provide novel validated DNA methylation markers associated with CIMP. Additional aspects provide novel methods and compositions for: determining CIMP status in colorectal cancers, determining the relationship between CIMP status and other molecular features of the cancers (e.g., BRAF mutation, KRAS mutation and MSI status); determining the relationship between CIMP status and other variables (e.g., age, sex, tumor location, family history, race, country of origin, tumor characteristics (including, tumor type, tumor grade, invasive margin characteristics, lymphocyte infiltration characteristics, direct spread, lymph node spread, venous spread and type of residual adjacent polyp, if present)); and determining, between subgroups defined by CIMP status and BRAF mutations, effects of selected risk factors (e.g., body mass index, smoking history, alcohol intake, dietary folate intake, folate metabolic enzyme polymorphisms and history of hormonal use). | 02-26-2009 |
| 20090053707 | Methods for lightening skin and hair - Methods are described wherein the skin of a vertebrate, or the skin or hair of a mammal can be lightened by administration of an agent, e.g., protein, peptide, active fusion protein, active fragment, or molecular mimic, that binds to BMP-4 transmembrane receptors on melanocytes and decreases the level of melanin synthesis. Also described are methods to identify molecules that mimic the function of BMP-4 in causing a decrease in melanin in melanocytes. | 02-26-2009 |
| 20090053708 | Method and/or Apparatus of Oligonucleotide Design and/or Nucleic Acid Detection - It is provided a method of designing at least one oligonucleotide for nucleic acid detection comprising the following steps in any order: (I) identifying and/or selecting region(s) of at least one target nucleic acid to be amplified, the region(s) having an efficiency of amplification (AE) higher than the average AE; and (II) designing at least one oligonucleotide capable of hybridizing to the selected region(s). It is also provided a method of detecting at least one target nucleic acid comprising the steps of: (i) providing at least one biological sample; (ii) amplifying nucleic acid(s) comprised in the biological sample; (iii) providing at least one oligonucleotide capable of hybridizing to at least one target nucleic acid, if present in the biological sample; and (iv) contacting the oligonucleotide(s) with the amplified nucleic acids and detecting the oligonucleotide(s) hybridized to the target nucleic acid(s). In particular, the method is for detecting the presence of at least one pathogen, for example a virus, in at least one human biological sample. The probes may be placed on a support, for example a microarray. | 02-26-2009 |
| 20090053709 | ENHANCED SENSITIVITY OF A CANTILEVER SENSOR VIA SPECIFIC BINDINGS - Detection of miniscule amounts of an analyte is accomplished via multiple bindings of specific materials on a sensor configured to sense mass. The sensor is prepared by immobilizing an antibody to a surface of the sensor, wherein the antibody is known to bind to the analyte. The prepared sensor is exposed to the analyte. The analyte binds to the antibody. The sensor then is exposed to additional antibody, which binds to the analyte. The sensor then can be sequentially exposed to additional antibodies that are known to bind to previously bound antibodies. Each additional binding further increases the effective mass of accumulated material on the sensor. The total effective mass is greater than the mass of the accumulated analyte, thus providing means for detecting extremely minute amounts of analyte. Applications include detection of pathogens and DNA. | 02-26-2009 |
| 20090053710 | FUNCTIONAL MOLECULE, FUNCTIONAL MOLECULE SYNTHESIZING AMIDITE AND TARGET SUBSTANCE ANALYSIS METHOD - To provide a functional molecule including a modified nucleotide unit having a substituent introduced to a base thereof, wherein the substituent is removably introduced to the base; a functional molecule synthesizing amidite that has a substituent removably introduced to its base and that is used for the manufacture of the functional molecule; and a target substance analysis method including: preparing a random pool of functional molecules using a functional molecule synthesizing amidite; screening a functional molecule having affinity for a target substance from the random pool; amplifying the functional molecules having affinity for the target substance, wherein the method further comprises, prior to the amplification step, removing a substituent from the functional molecule having affinity for the target substance. | 02-26-2009 |
| 20090053711 | PSEUDO-TISSUE FOR ACCURACY CONTROL, METHOD FOR CONTROLLING ACCURACY BY USING THE SAME, AND METHOD FOR MANUFACTURING THE SAME - The present invention provides a pseudo-tissue for accuracy control comprising a nucleic acid or cells, a holding body for holding the nucleic acid or cells, and a protecting body for covering at least a part of the surface of the holding body so as to protect the holding body. A method for controlling accuracy by using the pseudo-tissue, and a method for manufacturing the pseudo-tissue are also disclosed. | 02-26-2009 |
| 20090053712 | Methods of using LEDGF/p75 - The invention provides methods for diagnosing tumors in mammals using a reagent that bind to LEDGF/p75 or to a nucleic acid encoding LEDGF/p75. For example, the tumor may be located in the CNS, the prostate, the skin, the bone marrow, or the gut of the mammal. Also provided are methods for diagnosing brain tumors such as medulloblastomas, meningiomas, astrocytomas, glioblastomas multiforme, and ependymomas by examining LEDGF/p75 or a nucleic acid encoding LEDGF/p75 localization. The invention also involves methods for diagnosing cancers involving cancerous epithelial cells such as colon cancer. The instant invention also provides methods for isolating stem cells from a heterogeneous population of cells, as well as methods for identifying neuroepithelial stem cells, newly differentiated neurons, and astrocytes in a subject. Also provided are methods for inducing the differentiation of neuroepithelial stem cells into astrocytes or neurons and methods for screening candidate compounds that regulate the differentiation of neuroepithelial stem cells. | 02-26-2009 |
| 20090053713 | Isolated nucleic acids and polypeptides associated with glucose homeostasis disorders and method of detecting the same - An isolated and substantially pure nucleic acid sequence located between D20S119 and D20S178 on human chromosome 20q13, the nucleic acid sequence including: a nucleic acid sequence coding for a glucose transporting protein and having the sequence shown in SEQ ID NO: 1; or a nucleic acid sequence having at least 70% sequence identity with the nucleic acid sequence shown in SEQ ID NO: 1. The disclosed nucleic acid sequences map to a locus associated with human Type II diabetes mellitus and, therefore, therapeutic and diagnostic screening methods, which accommodate naturally and artificially occurring polymorphisms, are also disclosed. | 02-26-2009 |
| 20090053714 | Gins gene expression as marker for actively cycling cells and cell cycle phase - The invention relates to a method of detecting an actively cycling cell in a sample, said method comprising determining the state of GINS gene expression within said cell, wherein detection of GINS gene expression in said cell indicates that said cell is actively cycling. Furthermore, the invention relates to methods for detecting an actively cycling cell in a subject, said method comprising assaying a sample from said subject for evidence of GINS gene expression, in particular when the sample is a body fluid such as urine. Preferable the GINS gene is PSFI or SLD | 02-26-2009 |
| 20090053715 | Methods of screening nucleic acids for single nucleotide variations - Disclosed are methods and compositions for detecting variation in nucleic acids. The disclosed method compares the sequence of a nucleic acid of interest with the sequence of a reference nucleic acid to sensitively identify variations between the sequence of a nucleic acid of interest and the sequence of a reference nucleic acid. The disclosed method generally involves excision and replacement of selected nucleotides in nucleic acid strands hybridized to other strands. In the method, if the excised nucleotide was mismatched with the nucleotide in the other, hybridized strand, then the replacement nucleotide will not be mismatched. If the excised nucleotide was not mismatched with the nucleotide in the other, hybridized strand, then the excised nucleotide is not replaced. This difference allows detection of variation in the nucleic acid of interest. In some forms of the method, by replacing excised nucleotides with nuclease-resistant nucleotides, strands in which excised nucleotides are replaced will be resistant to nuclease digestion while strands in which excised nucleotides are not replaced will be sensitive to nuclease digestion. By exposing the hybridizing nucleic acids to nuclease following replacement of excised nucleotides, the strands in which excised nucleotides are not replaced can be destroyed by the nuclease while strands in which excised nucleotides are replaced can be preserved. The remaining strands can then be detected and whether the strand survived nuclease digestion can be noted. Strands that survive nuclease digestion are indicative of the presence of variation in the nucleic acid of interest. | 02-26-2009 |
| 20090053716 | METHOD OF DETECTING HUMAN CYTOCHROME P450 (CYP) 2D6 GENE MUTATION - A defect or multi-existence of a CYP2D6 gene is detected with a primer includes a complementary sequence to a sequence which is common between the CYP2D6 gene and a CYP2D8 gene but different from a CYP2D7 gene and which contains one or more of bases at the 86-, 90- and 93-positions in Exon | 02-26-2009 |
| 20090053718 | NOVEL OLIGONUCLEOTIDE COMPOSITIONS AND PROBE SEQUENCES USEFUL FOR DETECTION AND ANALYSIS OF microRNAs AND THEIR TARGET mRNAs - The invention relates to ribonucleic acids and oligonucleotide probes useful for detection and analysis of microRNAs and their target mRNAs, as well as small interfering RNAs (siRNAs). The invention furthermore relates to oligonucleotide probes for detection and analysis of other non-coding RNAs, mRNAs, mRNA splice variants, allelic variants of single transcripts, mutations, deletions, or duplications of particular exons in transcripts, e.g. alterations associated with human disease, such as cancer. | 02-26-2009 |
| 20090053719 | ANALYSIS OF NUCLEIC ACIDS BY DIGITAL PCR - The present invention provides a method for analyzing nucleic acids for their lengths and relative abundance in a sample, based on digital amplification of individual template molecules. This invention has many applications, including those in noninvasive prenatal diagnosis, transplantation monitoring, and the detection and monitoring of cancers and virus-associated diseases. | 02-26-2009 |
| 20090053720 | SUPPRESSION OF AMPLIFICATION USING AN OLIGONUCLEOTIDE AND A POLYMERASE SIGNIFICANTLY LACKING 5'-3' NUCLEASE ACTIVITY - Methods and compositions for amplification of a target sequence by suppressing amplification of related sequences are provided. | 02-26-2009 |
| 20090053721 | VOLTAGE-GATED ION CHANNEL MUTANTS FOR USE IN IDENTIFYING ION CHANNEL MODULATING COMPOUNDS - The present invention provides novel methods and compositions for identifying ion channel modulating compounds, including atrial-selective antiarrhythmic agents and ion channel modulating compounds that preferentially modulate K | 02-26-2009 |
| 20090053723 | Peripherin and Neurofilament Light Protein Splice Variants in Amyotrophic Lateral Sclerosis (ALS) - Nucleotide sequences encoding novel splice variants of peripherin and neurofilament light protein, proteins encoded by the novel splice variants and antibodies thereto are disclosed. In addition, methods are described for detecting ALS in a subject suspected of having ALS, comprising detecting the presence or absence of the novel splice variants or resulting proteins or a change in the amount of the novel splice variants or resulting proteins; wherein the presence or change in the amount of the nucleotide sequence is indicative of ALS. | 02-26-2009 |
| 20090053724 | System and method for adaptive reagent control in nucleic acid sequencing - An embodiment of a method for adaptive reagent control is described that comprising a) introducing a first concentration of an enzyme reagent into a reaction environment with a reaction substrate, where the enzyme reagent and reaction substrate are constituent parts of a sequencing process; b) measuring a level of activity of the first concentration of the enzyme reagent in the reaction environment, where the level of activity comprises a measurable product of a reaction between the enzyme reagent and the reaction substrate; c) identifying an optimal concentration using the measured level of activity of the first concentration; and d) performing the sequencing process in the reaction environment using the optimal concentration of the enzyme reagent, where the sequencing process comprises an iterative series of sequencing reactions. | 02-26-2009 |
| 20090053725 | Using DNA aptamers and quantum dots for the detection of proteins or other targets - The solutions provided here use DNA aptamers and quantum dots for the detection of bacteria, viruses, proteins or other targets. An example of a method described here comprises: providing a complex of DNA complementary strands, one strand being an aptamer, having one strand covalently linked to a quantum dot, and having the other strand linked to a quencher; and contacting said complex of DNA complementary strands with a microorganism or components thereof, under conditions that permit binding of said aptamer with said microorganism or components thereof. In some examples described here, the methods and systems are extremely simple to use and appear to have several advantages over the traditional ELISA. Since no blocking steps are required and the number of washing steps is reduced, the time required to conduct the test is greatly reduced. In some examples described here, a quantum dot aptamer complex comprises one strand of a duplex DNA molecule linked to the quantum dot by an amide bond. It does not matter if the aptamer or the complimentary strand is attached. However, the strand that is not attached contains a non-radiative quencher. Upon addition of the aptamers' target, the amount of light emitted by the quantum dots increases. In some examples described here, the methods and systems can also be used in reverse, with the aptamers' target immobilized on a microtiter plate. This permits an assay like a competitive immuno-assay. | 02-26-2009 |
| 20090053726 | SYSTEMS AND METHODS FOR REAL-TIME PCR - In one aspect, the present invention provides a systems and methods for the real-time amplification and analysis of a sample of DNA. | 02-26-2009 |
| 20090053727 | Risk Assessment For Adverse Drug Reactions - The present invention provides a method of predicting the risk of a patient for developing adverse drug reactions, particularly SJS or TEN. It was discovered that an HLA-B allele, HLA-B* 1502, is associated with SJS/TEN that is induced by a variety of drugs. The correlation with HLA-B* 1502 is most significant for carbamazepine-induced SJS/TEN, wherein all the patients tested have the HLA-B* 1502 allele. In addition, another HLA-B allele, HLA-B*5801, is particularly associated with SJS/TEN induced by allopurinol. Milder cutaneous reactions, such as maculopapular rash, erythema multiforme (EM), urticaria, and fixed drug eruption, are particularly associated with a third allele, HLA-B *4601. For any of the alleles, genetic markers (e.g., HLA markers, microsatellite, or single nucleotide polymorphism markers) located between DRB1 and HLA-A region of the specific HLA-B haplotype can also be used for the test. | 02-26-2009 |
| 20090053728 | Analytical Method and Kit - Analytical methods using RNA probes for the detection or analysis of nucleic acid sequences is described. These probes are contacted with a sample suspected of containing the nucleic acid sequence and if they form duplexes, they are hydrolysed. This may be done, for example during an amplification reaction. AMP generated as a result of the hydrolysis is converted to ATP. The ATP may then be detected using bioluminescent reagents. | 02-26-2009 |
| 20090053729 | METHOD FOR DETECTION OF MUTANT GENE - Disclosed is a method for detecting mutation(s) in nucleotide sequence, which comprises performing a nucleic acid amplification reaction by using an oligonucleotide or a salt thereof as a primer and a nucleic acid in a sample as a template and detecting a reaction product, wherein the oligonucleotide is so modified at the nucleotide at the second position from the 3′-terminus as to inhibit the nucleic acid synthesis. Also disclosed is a kit for the method. According to the present invention, since it is possible to completely eliminate any false positive result in the determination and correspond to various mutation patterns by a single run of PCR1 reaction, it becomes possible to design a drug-resistance determination system, which can detect possible plural genetic mutations by a single run of multiplex PCR. | 02-26-2009 |
| 20090061421 | CRYSTAL STRUCTURE OF CMY-10, A BETA-LACTAMASE CAUSING ANTIBIOTIC RESISTANCE WITH EXTENDED-SUBSTRATE SPECTRUM - The present invention related to a method for crystallizing a CMY-10 being a β-lactamase with extended-substrate spectrum, a crystal of CMY-10, and a crystal structure of CMY-10. With utilization of three-dimensional structure of CMY-10 protein provided by the present invention, it is possible to develop novel antibiotics or inhibitors that can prevent an emergence of resistance bacteria appeared by plasmidic class C β-lactamases having extended-substrate specificity. | 03-05-2009 |
| 20090061422 | Diagnostic markers of breast cancer treatment and progression and methods of use thereof - To maximize both the life expectancy and quality of life of patients with operable breast cancer, it is important to predict adjuvant treatment outcome and likelihood of progression before treatment. The instant invention details the usage of a machine-learning based method to develop a cross-validated model to predict the outcome of adjuvant treatment, particularly chemotherapy treatment outcome, and likelihood of progression before treatment. The model includes standard clinicopathological features, as well as molecular markers collected using standard immunohistochemistry and fluorescence in situ hybridization. The model significantly outperformed the St. Gallen Consensus guidelines and the Nottingham Prognostic Index and has the potential to provide a clinically useful and cost-effective prognostic for breast cancer patients. | 03-05-2009 |
| 20090061423 | PHARMACOGENOMIC MARKERS FOR PROGNOSIS OF SOLID TUMORS - The present invention provides methods, systems and equipment for prognosis or evaluation of treatment of solid tumors. Gene markers that are prognostic of solid tumors can be identified according to the present invention. Each gene marker has altered expression patterns in PBMCs of solid tumor patients following initiation of an anti-cancer treatment, and the magnitudes of these alterations are correlated with clinical outcomes of these patients. In one embodiment, a Cox proportional hazards model is used to determine the correlations between clinical outcomes of RCC patients and gene expression changes in PBMCs of these patients during the course of a CCI-779 treatment. Non-limiting examples of genes identified by the Cox model are depicted in Tables 4A3 4B, 5 A and 5B. These genes can be used as surrogate markers for prognosis of RCC. They can also be used as pharmacogenomic indicators for the efficacy of CCI-779 or other anti-cancer drugs. | 03-05-2009 |
| 20090061424 | UNIVERSAL LIGATION ARRAY FOR ANALYZING GENE EXPRESSION OR GENOMIC VARIATIONS - The present invention provides an array system comprising a plurality of immobilized oligonucleotides comprising artificial sequences and a plurality of complementary ligation templates, as well as methods and kits for using the array system to analyze populations of nucleic acids. In particular, target nucleic acids are ligated to the immobilized oligonucleotides on the array in the presence of the complementary ligation templates. | 03-05-2009 |
| 20090061425 | Methods and kits for selectively amplifying, detecting or quantifying target DNA with specific end sequences - Disclosed herein are methods and kits for selectively amplifying, detecting or quantifying a DNA fragment with a specific end sequence, especially generated following restriction enzyme digestion. This method can be used, for example, to detect a hypomethylated DNA fragment. This methods and kits are especially useful in detecting or quantifying a hypomethylated fetal DNA fragment in a maternal plasma sample containing a corresponding hypermethylated maternal DNA fragment. | 03-05-2009 |
| 20090061426 | Binary signaling assay using a split-polymerase - The present invention provides methods, kits and compositions for the detection of an analyte. In the methods of the invention, a complex is formed between two or more analyte specific probes (ASP) and an analyte. The analyte specific probes each have a portion of a polymerase which interact to form a functional polymerase complex upon binding of the ASP to the analyte. The functional polymerase complex then generates a detectable signal which is indicative of the presence and/or amount of the analyte in the sample. | 03-05-2009 |
| 20090061427 | Gene Encoding Protein Responsible for Flocculation Property of Yeast and Use Thereof - The present invention relates to a gene encoding a protein responsible for flocculation property of yeast and use thereof in particular, a brewery yeast with appropriate flocculation property for producing desired alcoholic beverages, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast to which suitable flocculation property for brewing desired alcoholic beverages was imparted by controlling expression level of HSP150 gene encoding a protein responsible for flocculation property of brewery yeast, especially non-ScHSP150 gene specific to a lager brewing yeast, to alcoholic beverages produced with said yeast and to a method for producing said beverages. | 03-05-2009 |
| 20090061428 | Thermal Reaction Device and Method for Using the Same - An M times.N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block. | 03-05-2009 |
| 20090061429 | Reactive surfaces, substrates and methods of producing and using same - Reactive surfaces, substrates and methods of producing and using such substrates and surfaces are provided. The substrates and surfaces provide low density reactive groups preferably on an otherwise non-reactive surface for use in different applications including single molecule analyses. | 03-05-2009 |
| 20090061430 | Reactive surfaces, substrates and methods of producing and using same - Reactive surfaces, substrates and methods of producing and using such substrates and surfaces are provided. The substrates and surfaces provide low density reactive groups preferably on an otherwise non-reactive surface for use in different applications including single molecule analyses. | 03-05-2009 |
| 20090061431 | Method of prognosing and diagnosing hereditary spastic paraplegia, mutant nucleic acid molecules and polypeptides - A method for diagnosing the presence of hereditary spastic paraplegia (HSP) or predicting the risk of developing HSP in a human subject, comprising detecting the presence or absence of a defect in a gene encoding a polypeptide comprising the sequence of FIG. | 03-05-2009 |
| 20090061432 | CSPCNA ISOFORM MODIFICATIONS AND USES THEREOF - Methods and compositions to detect the presence of csPCNA isoform by identifying one or more posttranslational modifications are disclosed. Methods to identify csPCNA isoform through posttranslational modifications including methylesterification levels are disclosed. | 03-05-2009 |
| 20090061433 | NUCLEOTIDE PRIMER SET AND NUCLEOTIDE PROBE FOR DETECTING GENOTYPE OF SERUM AMYLOID A1(SAA1) - Provided is a LAMP-amplification nucleotide primer set for detection of the genotype of single nucleotide polymorphisms C-13T, C2995T and T3010C of the SAA1 gene. Also provided is a nucleotide probe for detection of the amplification product amplified with the primer set according to the present invention. Further provided is a method of detecting the genotype of the single nucleotide polymorphisms C-13T, C2995T and T3010C of the SAA1 gene by using the primer set according to the present invention. | 03-05-2009 |
| 20090061434 | PRIMER COMPOSITION AND A KIT USEFUL FOR THE IDENTIFICATION OF PHYLLANTHUS - The present invention relates to an oligonucleotide primer set useful for the identification of | 03-05-2009 |
| 20090061435 | Methods and compositions for rapid amplification, capture and detection of nucleic acids and proteins - A method for detecting the presence of a nucleic acid template ( | 03-05-2009 |
| 20090061436 | PEPTIDE SEQUENCE THAT PROMOTES TUMOR INVASION - An isolated sequence SGSSEEKQNAVSSEET (OPNcPEP) SEQ ID NO: 8, and uses thereof. The peptide enhanced soft agar clone formation but did not support the growth of cells in plastic dishes, consistent with supporting anchorage-independence rather than growth. This sequence represented and is unique for a domain around the splice junction of OPN variant -c (OPN-c). OPN-c was expressed in a variety of tumor cell lines, but not in normal tissues (e.g., non-cancerous tissue) or in benign tumors. OPN-c antibody may be administered to a patient with a cancer associated with OPN-c expression to prevent the formation and growth of metastases. OPN-c may be used as a diagnostic to determine whether a patient has a malignant, rather than a benign, growth. OPN-c may be used to detect or identify agents that inhibit or mimic OPN-c expression or activity. | 03-05-2009 |
| 20090061437 | Nucleotide Analogs - The invention provides for nucleotide analogs and methods of using the same, e.g., for sequencing nucleic acids. | 03-05-2009 |
| 20090061438 | DETECTION OF MICROSATELLITE INSTABILITY AND ITS USE IN DIAGNOSIS OF TUMORS - Methods and kits are disclosed for use in the analysis of microsatellite instability in genomic DNA. Methods and kits are also disclosed which can be used to detect microsatellite instability DNA present in biological materials, such as tumors. The methods and kits of the present invention can be used to detect or diagnose diseases associated with microsatellite instability, such as certain types of cancer. | 03-05-2009 |
| 20090061439 | Methods and Compositions for Sequencing A Nucleic Acid - The invention provides nucleotide analogs and methods of using them in sequencing reactions. | 03-05-2009 |
| 20090061440 | METHOD FOR AMPLIFYING PLURAL NUCLEIC ACID SEQUENCES FOR DISCRIMINATION - The invention provides a method with fewer distribution of the amount of amplification among plural tags when the plural tags are used for multiplex detection using the tag. | 03-05-2009 |
| 20090061441 | EPIGENETIC SILENCING OF CYCLOOXYGENASE-2 AFFECTS CLINICAL OUTCOME IN GASTRIC CANCER - The present invention discloses methods of using the methylation status of the COX-2 gene promoter region as a biomarker for a gastric cancer patient to determine a prognosis and a treatment regimen, and to monitor the progress of a treatment regimen. | 03-05-2009 |
| 20090061442 | Screening assay to identify non-ATP-competitors targeting protein kinase A - Methods for screening compounds for their activity as a protein kinase A modulators are provided. The methods are based on fluorescence polarization of peptide probes to identify drug candidates that act by activating or inhibiting the catalytic function of PKA. In certain embodiments, the methods are adapted for high throughput screening. | 03-05-2009 |
| 20090061443 | Diagnostic and Therapeutic Targets for Leukemia - The present invention relates to methods of identifying candidate compounds for the treatment of leukemia and diagnostic methods based on histone methylation and HoxA5 promoter activity. | 03-05-2009 |
| 20090061444 | PHYTOCHROME-BASED FLUOROPHORES - Genetically-engineered fluorophore molecules with increased fluorescence are provided. These fluorophores are derived from the domains of phytochromes, and in particular bacterial phytochromes. Methods for generating these fluorophores and various applications of these fluorophores are also provided. | 03-05-2009 |
| 20090061445 | FLUX BALANCE ANALYSIS WITH MOLECULAR CROWDING - Methods are provided herein for: calculating cell growth rates in various environments and genetic backgrounds; calculating the order of substrate utilization from a defined growth medium; calculating metabolic flux reorganization in various environments and at various growth rates; and calculating the maximum metabolic rate and optimal metabolite concentrations and enzyme activities by applying a computational optimization method to a kinetic model of a metabolic pathway. The optimization methods use intracellular molecular crowding parameters and/or well as kinetic rates to assist in modeling metabolic activity. | 03-05-2009 |
| 20090061446 | METHOD FOR QUICKLY IDENTIFYING PATHOGENIC BACTERIA RESPONSIBLE FOR INFECTION - A system rapidly detects and identifies pathogenic bacteria responsible for infection (particularly septicemia), and selects an appropriate antimicrobial drug. A method according to the present invention for detecting and identifying pathogenic bacteria includes performing gene amplification such as real-time PCR, and analyzing the combination of the melting temperatures (Tm values) determined by gene amplification product melting curve analysis or the difference between the Tm values. Specifically, real-time PCR is performed using 4 to 16 primer sets including 1 to 7 primer sets for the 16S ribosomal RNA of bacteria, 1 to 6 primer sets for the 18S ribosomal RNA of fungi, and one primer set respectively for the spa gene and the mecA gene specific to MRSA, and the combination of the Tm values of the amplification product or the combination of the differences between the Tm values is compared with a database to identify pathogenic bacteria responsible for septicemia. Pathogenic bacteria responsible for infection (particularly septicemia) can be rapidly detected and identified using the method according to the invention so that a rapid septicemia diagnosis method and evidence-based medicine in septicemia treatment are implemented. | 03-05-2009 |
| 20090061447 | HIGH SPEED PARALLEL MOLECULAR NUCLEIC ACID SEQUENCING - A method and device is disclosed for high speed, automated sequencing of nucleic acid molecules. A nucleic acid molecule to be sequenced is exposed to a polymerase in the presence of nucleotides which are to be incorporated into a complementary nucleic acid strand. The polymerase carries a donor fluorophore, and each type of nucleotide (e.g. A, T/U, C and G) carries a distinguishable acceptor fluorophore characteristic of the particular type of nucleotide. As the polymerase incorporates individual nucleic acid molecules into a complementary strand, a laser continuously irradiates the donor fluorophore, at a wavelength that causes it to emit an emission signal (but the laser wavelength does not stimulate the acceptor fluorophore). In particular embodiments, no laser is needed if the donor fluorophore is a luminescent molecule or is stimulated by one. The emission signal from the polymerase is capable of stimulating any of the donor fluorophores (but not acceptor fluorophores), so that as a nucleotide is added by the polymerase, the acceptor fluorophore emits a signal associated with the type of nucleotide added to the complementary strand. The series of emission signals from the acceptor fluorophores is detected, and correlated with a sequence of nucleotides that correspond to the sequence of emission signals. | 03-05-2009 |
| 20090061448 | METHOD FOR IDENTIFYING OCULOSKELETAL DYSPLASIA IN DOGS - Provided are methods for identifying dogs as likely to be genetically normal, carriers of, or affected with Oculo-skeletal dysplasia (OSD) by determining the presence or absence of a drd2 COL9A2 mutation and/or a drd1 COL9A3 mutation. Also provided is a method for selective breeding of dogs and kits useful for carrying out the methods of the invention. | 03-05-2009 |
| 20090061449 | SYSTEMS AND METHODS FOR PROCESSING HYBRID SEED - The present disclosure provides for systems and method for producing hybrid seed. In various embodiments, the disclosure provides a system for the high-throughput, nondestructive sampling of seeds. In another embodiment, a high-throughput, nondestructive method for producing hybrid seeds comprises removing a sample from a plurality of seeds in the population while preserving the germination viability of the seed and analyzing the sample for the presence or absence of one or more genetic markers indicative of a male-sterile gene. | 03-05-2009 |
| 20090061450 | SYSTEM AND METHOD FOR DIAGNOSIS OF INFECTIOUS DISEASES - A biosafe apparatus is disclosed for assay and diagnosis of respiratory pathogens comprising a nasal sampling device, a single entry, disposable microfluidic cartridge for target nucleic acid amplification, and an instrument with on-board assay control platform and target detection means. | 03-05-2009 |
| 20090061451 | BIOSENSORS AND RELATED METHODS - Provided herein are biosensors that comprise a biological signal source linked to a substrate by a peptide nucleic acid spacer and methods of use of the biosensor. In one embodiment, the biosensor is used to detect prostate-specific antigen. | 03-05-2009 |
| 20090061452 | Polymorphisms in the human genes for OCT 1 and their use in diagnostic and therapeutic applications - The present invention relates to a polymorphic OCT1 polynucleotide. Moreover, the invention relates to genes or vectors comprising the polynucleotides of the invention and to a host cell genetically engineered with the polynucleotide or gene of the invention. Further, the invention relates to methods for producing molecular variant polypeptides or fragments thereof, methods for producing cells capable of expressing a molecular variant polypeptide and to a polypeptide or fragment thereof encoded by the polynucleotide or the gene of the invention or which is obtainable by the method or from the cells produced by the method of the invention. Furthermore, the invention relates to an antibody which binds specifically the polypeptide of the invention. Moreover, the invention relates to a transgenic non-human animal. The invention also relates to a solid support comprising one or a plurality of the above mentioned polynucleotides, genes, vectors, polypeptides, antibodies or host cells. Furthermore, methods of identifying a polymorphism, identifying and obtaining a pro-drug or drug or an inhibitor are also encompassed by the present invention. In addition, the invention relates to methods for producing of a pharmaceutical composition and to methods of diagnosing a disease. Further, the invention relates to a method of detection of the polynucleotide of the invention. Furthermore, comprised by the present invention are a diagnostic and a pharmaceutical composition. Even more, the invention relates to uses of the polynucleotides, genes, vectors, polypeptides or antibodies of the invention. Finally, the invention relates to a diagnostic kit. | 03-05-2009 |
| 20090061453 | Methods and compositions for detecting colon cancers - This application describes methods and compositions for detecting and treating HLTF-associated neoplasia. Differential methylation of the HLTF nucleotide sequences has been observed in HLTF-associated neoplasia such as colon neoplasia. | 03-05-2009 |
| 20090061454 | DIAGNOSTIC AND PROGNOSTIC METHODS FOR LUNG DISORDERS USING GENE EXPRESSION PROFILES FROM NOSE EPITHELIAL CELLS - The present invention provides methods for diagnosis and prognosis of lung cancer using expression analysis of one or more groups of genes, and a combination of expression analysis from a nasal epithelial cell sample. The methods of the invention provide far less invasive method with a superior detection accuracy for lung cancer when compared to any other currently available method for lung cancer diagnostic or prognosis. The invention also provides methods of diagnosis and prognosis of other lung diseases, such as lung cancer. | 03-05-2009 |
| 20090068641 | Highly conserved genes and their use to generate probes and primers for detection of microorganisms - Four highly conserved genes, encoding translation elongation factor Tu, translation elongation factor G, the catalytic subunit of proton-translocating ATPase and the RecA recombinase, are used to generate species-specific, genus-specific, family-specific, group-specific and universal nucleic acid probes and amplification primers to rapidly detect and identify algal, archaeal, bacterial, fungal and parasitical pathogens from clinical specimens for diagnosis. The detection of associated antimicrobial agents resistance and toxin genes are also under the scope of the present invention. | 03-12-2009 |
| 20090068642 | Composition and pharmacology of novel alpha6-containing nicotinic acetylcholine receptors - Nicotinic acetylcholine receptors (nAChRs) comprising the α6 receptor subunit; nucleic acids, including vectors, comprising subunit incoding sequences; cells expressing the nAChRs of the invention; and methods of screening compounds are provided. | 03-12-2009 |
| 20090068643 | Dual Function Primers for Amplifying DNA and Methods of Use - The present invention provides novel nucleotide compositions that enable the detection of DNA synthesis products and methods for use thereof. In one embodiment, the method can be used in PCR and allows the progress of the reaction to be monitored as it occurs. In one embodiment, the invention employs at least one fluorescence-quenched oligonucleotide that can prime DNA extension reactions. In a second embodiment, the invention employs at least one fluorescence-quenched oligonucleotide that can function as a template for DNA extension reactions. In both embodiments, the oligonucleotide also functions as a probe for detecting the progress of successive extension reaction cycles. Signal detection is dependent upon DNA synthesis and can occur with or without probe cleavage. | 03-12-2009 |
| 20090068644 | METHOD FOR PRODUCING HIGHLY SENSITIVE ENDONUCLEASES, NOVEL PREPARATIONS OF ENDONUCLEASES AND USES THEREOF - The present invention pertains to methods for producing recombinant endonucleases having a high sensitivity, as well as to endonucleases preparations obtained by said methods, and uses thereof, especially for the detection of mismatches. | 03-12-2009 |
| 20090068645 | Labeling and Sequencing of Nucleic Acids - The invention provides for methods of end labelling a ds DNA molecule with a bar-code as well as adaptor mediated methodology for creating single stranded overhanging ends, lengthening single stranded overhanging ends, controlled size reduction of labelled ds DNA molecules, and adaptor mediated sequencing. Also disclosed are methods for parallel sequencing of multiple ds DNA molecules in a mixture by visual means. | 03-12-2009 |
| 20090068646 | METHODS AND KITS FOR DETECTING MUTATIONS - Disclosed are methods and kits for detecting mutations in DNA by comparing the size of an amplified microsatellite locus to the expected size. The methods and kits may used in various applications, including monitoring exposure of a cell or organism to a mutagen, evaluating the mutagenicity of an agent, and evaluating a putative precancerous or cancerous cell or tumor cell for microsatellite instability. | 03-12-2009 |
| 20090068647 | Dna sequences for the detection of and differentiation amongst pathogenic e.coli - Oligonucleotide sequences and methods for specifically detecting and differentiating amongst pathogenic | 03-12-2009 |
| 20090068648 | Method and system for determining a quality metric for comparative genomic hybridization experimental results - Various embodiments of the present invention determine various quality metrics that reflect the quality of two or more identically-executed or similar array-based comparative-genomic-hybridization (“aCGH”) experiments. In certain embodiments of the present invention, a pairwise quality metric is generated for each possible pair of aCGH experimental results within a set of aCGH experimental results. The pairwise quality metrics may be summed and optionally normalized to produce an overall quality metric for the set of aCGH experimental results. Various pairwise quality metrics can be used in different embodiments of the present invention, including pairwise quality metrics based on measures of aberration overlap. | 03-12-2009 |
| 20090068649 | Methods of DNA Methylation Detection - The present invention provides for methods of DNA methylation detection. The present invention provides for methods of generating and detecting specific electronic signals that report the methylation status of targeted DNA molecules in biological samples. | 03-12-2009 |
| 20090068650 | Metabolic Primers for the Detection of (Per) Chlorate-Reducing Bacteria and Methods of Use Thereof - The present invention is directed to metabolic primers for the detection of (per)chlorate-reducing bacteria and methods and compositions for use of the same in environmental bioremediation. | 03-12-2009 |
| 20090068651 | Method for the detection of the antibiotic resistance spectrum of mycobacterium species - Method for the detection of the antibiotic resistance spectrum of | 03-12-2009 |
| 20090068652 | Method for identifying the sequence of one or more variant nucleotides in a nucleic acid molecule - The invention relates to methods for identifying the sequence of one or more variant nucleotides in a nucleic acid molecule. The method involves cleaving a double-stranded nucleic acid molecule containing a mismatch with a mismatch-specific endonuclease which cleaves on the 3′ side of the mismatch, and preserving the integrity of the variant nucleotide by ligating a double-stranded linker with a 3′-overhang to said variant nucleotide. Because the variant nucleotide is immediately adjacent to the linker, PCR and/or sequence-by-synthesis analysis can be readily carried out. | 03-12-2009 |
| 20090068653 | BIOCHEMICAL AND GENETIC ANALYSIS FOR PREDICTION OF BREAST CANCER RISK - The present invention provides new methods for the assessment of cancer risk in the general population. These methods utilize particular alleles of three selected genes, here associated with specific biochemical activities, to identify individuals with increased or decreased risk of breast cancer. Using such methods, it is possible to reallocate healthcare costs in cancer screening to patient subpopulations at increased cancer risk and to identify candidates for cancer prophylactic treatment. | 03-12-2009 |
| 20090068654 | ASSAY FOR IDENTIFYING COMPOUNDS WHICH AFFECT STABILITY OF MRNA - The present invention relates to an assay for the identification of biologically active compounds, in particular to a reporter gene assay for the identification of compounds, which have an effect on mRNA stability. More particularly, the present invention relates to a reporter gene expression system and cell lines comprising said expression system. The invention further relates to compounds which destabilise mRNA. | 03-12-2009 |
| 20090068655 | COMPOSITION AND METHOD FOR NUCLEIC ACID SEQUENCING - The present invention provides compositions and methods for detecting incorporation of a labeled nucleotide triphosphate onto the growing end of a primer nucleic acid molecule. The method is used, for example, to genotype and sequence a nucleic acid. In a preferred embodiment, the method described herein detects individual NTP molecules. | 03-12-2009 |
| 20090068656 | Methods of diagnosing osteoarthritis - A method of diagnosing osteoarthritis in a mammal is provided comprising the steps of obtaining a biological sample from the mammal; and quantifying in the sample the expression of at least one chondrocyte-specific gene or gene product, wherein a differential in expression of said gene or gene product in comparison with expression of said gene or gene product in a non-osteoarthritic mammal is indicative of osteoarthritis. | 03-12-2009 |
| 20090068657 | METHOD OF PREDICTING THE CLINICAL RESPONSE TO CISPLATIN OR CARBOPLATIN CHEMOTHERAPEUTIC TREATMENT - Method for predicting the survival of a patient suffering from NSCLC to a cisplatin or carboplatin based chemotherapy treatment which comprises the step of determining the methylation state of a nucleic acid encoding 14-3-3 sigma in a biological sample from the patient, wherein the presence of methylation is indicative of longer survival of said patient as a response to said chemotherapy treatment. The methylation status of the 14-3-3 sigma gene can be easily determined in a serum sample. | 03-12-2009 |
| 20090068658 | Soybean mutant strain and soybean oil therefrom - A soybean oil of a decreased content of polyunsaturated fatty acids is produced by hybridizing, selecting and raising a soybean mutant KK21 characterized by a gene having a sequence of base given by the sequence No. 1 of the sequence listing, a soybean mutant M23 characterized by deletion of a gene having a sequence of base given by the sequence No. 2 of the sequence listing, a soybean mutant M24 characterized by a gene having a sequence of base given by the sequence No. 3 of the sequence listing, a soybean mutant M5 characterized by a gene having a sequence of base given by the sequence No. 4 of the sequence listing, a soybean mutant J18 characterized by deletion of a gene having a sequence of base given by the sequence No. 5 of the sequence listing, and one or two kinds or more of these soybean mutants. | 03-12-2009 |
| 20090068659 | Method for identifying the sequence of one or more variant nucleotides in a nucleic acid molecule - The invention relates to methods for identifying the sequence of one or more variant nucleotides in nucleic acid molecules. The method involves cleaving a double-stranded nucleic acid molecule containing a mismatch with a mismatch-specific endonuclease which cleaves on the 3′ side of the mismatch, and preserving the integrity of the variant nucleotide by ligating a Double-Stranded Linker with a degenerate 3′-overhang to said variant nucleotide. Because the variant nucleotide is immediately adjacent to the linker, PCR and/or sequence-by-synthesis analysis can be readily carried out. | 03-12-2009 |
| 20090068660 | USE OF METHYLATED OR UNMETHYLATED LINE-1 DNA AS A CANCER MARKER - The invention relates to a method of detecting LINE-1 (long interspersed nucleotide elements-1) DNA either methylated or unmethylated at the promoter region in a tissue or body fluid sample from a subject. Also disclosed are methods of using LINE-1 DNA as a biomarker for diagnosing, predicting, and monitoring cancer progression and treatment. | 03-12-2009 |
| 20090068661 | PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD - A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 4 and mutated sequences thereof. | 03-12-2009 |
| 20090068662 | METHOD AND TEST KIT FOR THE SEPARATION, PURIFICATION AND RECYCLING OF LONG- AND SHORT-CHAIN NUCLEIC ACIDS - Long- and/or short-chain nucleic acids are separated, purified and recovered by binding the nucleic acid to a solid phase using a binding buffer, to obtain a bonded nucleic acid, and eluting of the bonded nucleic acid from the solid phase, wherein the binding buffer comprises at least one citric acid salt and at least one alcohol. | 03-12-2009 |
| 20090068663 | SCREENING METHODS AND SEQUENCES RELATING THERETO - A screening method for identifying an individual having a pre-disposition towards having a cancer is disclosed, which screening method comprises the steps of:
| 03-12-2009 |
| 20090068664 | AMPLIFICATION METHODS AND COMPOSITIONS - The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions. | 03-12-2009 |
| 20090068665 | METHODS AND KITS FOR IDENTIFYING TARGET NUCLEOTIDES IN MIXED POPULATIONS - Ligation-based methods and kits are disclosed for identifying at least two target nucleotides in a mixed population sample that is a sample that contains or potentially contains target nucleic acid sequences from more than one source. Typically, two ligation reaction compositions are formed ligation products generated, and the ligation products or their surrogates are analyzed to identify target nucleotides in the mixed population sample. In certain embodiments, the target nucleic acid sequences, the ligation products, or both are amplified. In certain embodiments, multiplex amplification and/or ligation reactions are performed. | 03-12-2009 |
| 20090068666 | Systems and Methods for Determining an Amount of Starting Reagent using the Polymerase Chain Reaction - Systems and methods for calculating an initial amount of target nucleic acid N | 03-12-2009 |
| 20090068667 | METHODS AND ASSAYS FOR SCREENING STEM CELLS - The present invention provides methods and assays for screening cells, such as stem cells, for chromosomal aberrations. In particular, the present invention provides a rapid, sensitive assay platform for detecting high and low levels of chromosomal aberrations present in a cell population. This includes, but is not limited to, detection of extra chromosomes (trisomies) as well as insertions of small segments that are undetectable using standard cytogenetic studies, wherein the abnormal cells comprise a low percentage of the total cell population. | 03-12-2009 |
| 20090068668 | Waveguide-Based Optical Scanning Systems - A scanning sensor system, methods of use and kits for detecting a biologically active analyte are provided. The scanning sensor system includes a light source, a detector, a substrate comprising a plurality of waveguides and a plurality of optical sensing sites in optical communication with one or more waveguide of the substrate, and at least one adapter configured to couple with the substrate and provide optical communication between the light source, the waveguides of the substrate, and the detector. | 03-12-2009 |
| 20090068669 | SLC9A3R1 DIRECTED DIAGNOSTICS FOR NEOPLASTIC DISEASE - Disclosed are methods for diagnosing cancer in a test cell sample or fluid sample by detecting an increase in the level of expression of SLC9A3R1 in the test cell sample or fluid sample as compared to the level of expression of SLC9A3R1 in a control cell sample or fluid sample isolated from a normal subject. | 03-12-2009 |
| 20090068670 | STRATIFIN DIRECTED DIAGNOSTICS FOR NEOPLASTIC DISEASE - Disclosed are methods for diagnosing cancer in a test cell sample or fluid sample by detecting an increase in the level of expression of stratifin in the test cell sample or fluid sample as compared to the level of expression of stratifin in a control cell sample or fluid sample isolated from a normal subject. | 03-12-2009 |
| 20090068671 | Binding method and apparatus for sorting objects - The present invention relates to a method and apparatus of sorting objects including, providing a sample having wanted objects and unwanted objects; coating a surface of a sample holder with an antibody; placing an eluted sample on the sample holder; binding an antigen in the wanted objects with the antibody on the surface of the sample holder to sort the objects into wanted objects and unwanted objects; separating the wanted objects; and performing PCR-based STR analysis on the wanted objects. In one embodiment, holographic optical trapping is used to further sort the wanted objects. In other embodiments, the wanted objects are sperm and the antibody is a human sperm specific antibody, and the PCR is single cell PCR-based STR analysis. In still other embodiments, the binding is direct or indirect, ligands are used to bind to object-specific organomolecules, and protein A or protein G are used to bind the antibody. | 03-12-2009 |
| 20090068672 | DETECTION SYSTEM - The use of a red nucleic acid stain, in particular red fluorescent SYTO® dye in various methods used for the detection or characterisation of nucleic acids is described. In particular, the red nucleic acid stains have been found to be particularly compatible with the polymerase chain reaction (PCR), and therefore form the basis of enhanced detection methods. | 03-12-2009 |
| 20090068673 | UTILIZATION OF COMPATIBLE SOLUTES TO IMPROVE THE PERFORMANCE OF THE TECHNIQUES USING IMMOBILIZED BIOLOGIC MATERIALS - This invention is based upon the application of compatible solutes to immobilized biological materials or to immobilize in order to improve the technical performance using the same, namely nucleic acid and proteins or biosensors micronets. The above mentioned compatible solutes are selected from a group that includes Mannosylglycerate (MG) and its 3 derivatives manno-sylglyceramide (MGA), -mannosylglycerol (MGOH), and mannosyl-lactate (MGLac), 1,1-di-glycerol phosphate (DGP), ectoin, hydroxyectoin, and or derivatives or combinations thereof. The improved performance of the above mentioned techniques might be due to: a better state of preservation of the material to be immobilized, increasing the life expectancy of the material, and/or decreasing the storage and/or transport requirements, a more efficient and uniform deposition of the material on the support, more efficiency and/or specificity of hybridation of the marked material, all this originating an increase of the signal-to-noise ratio. | 03-12-2009 |
| 20090068674 | Method and device for preparing a sample of biological origin in order to determine at least one constituent contained therein - The invention relates to a method of preparing an original sample ( | 03-12-2009 |
| 20090068675 | Methods for improved diagnosis of dysplasias - The present invention relates to a method for improved diagnosis of dysplasias based on simultaneous detection of INK4a gene products and at least one marker for cell proliferation. Particularly the present invention provides a method for discriminating dysplastic cells over-expressing INK4a gene products from cells over-expressing INK4a gene products without being dysplastic by detection of a marker suitable for characterising the proliferation properties of the respective cell. The characterisation of the proliferation properties may comprise the detection of a marker or a set of markers characteristic for active cell proliferation and/or a marker or a set of markers characteristic for retarded or ceased cell proliferation. The method presented herein thus enables for a specific diagnosis of dysplasias in histological and cytological specimens. | 03-12-2009 |
| 20090075251 | Method for analysis of cytosine methylation - A method for the analysis of cytosine methylations in DNA is described. Here, the DNA to be investigated is first chemically or enzymatically converted. Then a promoter is introduced into the DNA. After this, the DNA is converted to RNA. The methylation pattern of the DNA can be concluded in different ways by means of an analysis of the RNA. The RNA is preferably fragmented chemically or enzymatically prior to the analysis, whereby the fragmenting results depend on the methylation pattern of the DNA. The method according to the invention is particularly suitable for the diagnosis and prognosis of cancer disorders and other diseases associated with a modification of the methylation pattern. | 03-19-2009 |
| 20090075252 | Methods for increasing accuracy of nucleic acid sequencing - The invention provides methods for improving the fidelity of a sequencing-by-synthesis reaction by resequencing at least a portion of a nucleic acid template. | 03-19-2009 |
| 20090075253 | BIOREACTIVE AGENTS - This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. Useful compositions include biological agents such as nucleic acids, proteins, lipids and cytokines. Conjugates can also be used to monitor the pathway and half-life of pharmaceutical composition in vivo and for diagnostic, therapeutic and prophylactic purposes. The invention also relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and nearly any individual substance in a complex background of substances. | 03-19-2009 |
| 20090075254 | PHYSIOGENOMIC METHOD FOR PREDICTING DIABETES AND METABOLIC SYNDROMES INDUCED BY PSYCHOTROPIC DRUGS - The invention is generally directed to a physiogenomic method for predicting diabetes and metabolic syndromes induced by psychotropic drugs. In one embodiment, the invention relates to the use of genetic variants of marker genes to predict the likelihood that an individual will experience undesirable metabolic side effects as a result of the use of a drug including, but not limited to, psychotropic drugs. The invention also relates to methods predicting the likelihood of diabetes and metabolic syndromes induced by the use of drugs with undesirable metabolic side effects. | 03-19-2009 |
| 20090075255 | Androgen regulated nucleic acid molecules and encoded proteins - The present invention provides novel androgen regulated nucleic acid molecules. Related polypeptides and diagnostic methods also are provided. | 03-19-2009 |
| 20090075256 | Nucleic Acid Accessible Hybridization Site Identification Using Mass Spectrometry - The present invention relates to methods and compositions for analyzing nucleic acids, and in particular, methods and compositions for detection and characterization of nucleic acid sequences and sequence changes using mass spectrometry. The present invention also provides methods and compositions for identifying oligonucleotides with desired hybridization properties to nucleic acid targets containing secondary structure using mass spectrometry. | 03-19-2009 |
| 20090075257 | NOVEL NUCLEIC ACID SEQUENCES AND METHODS OF USE THEREOF FOR DIAGNOSIS - Novel splice variant nucleic acid and amino acid sequences are provided. The novel splice variants and their nucleic acid sequences may be used for diagnosis of variant-detectable diseases particularly cancerous diseases. | 03-19-2009 |
| 20090075258 | Methods of Normalization in microRNA Detection Assays - This application describes methods of quantifying a target miRNA in a biological sample by measuring the amounts of a target miRNA and at least one reference oncomir in a reaction volume, and normalizing the amount of target miRNA to the amount of one or more reference oncomirs. | 03-19-2009 |
| 20090075259 | Analyte detection via antibody-associated enzyme assay - The present invention provides methods, kits, and compositions for the detection of an analyte. The invention is particularly suited for the detection and quantification of an analyte in a sample. In the methods of the invention a complex is formed between an analyte specific binding agent and an analyte. The analyte specific agents are coupled to an enzyme possessing an activity that produces a PCR template indicative of the presence of the analyte. Amplification and detection of the PCR template yields a sensitive and quantitative measurement of analyte concentration. | 03-19-2009 |
| 20090075260 | Methods and Nucleic Acids For Analysis of Cellular Proliferative Disorders - Aspects of the invention provide methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. Particular aspects disclose and provide genomic sequences the methylation patterns of which have substantial utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients. | 03-19-2009 |
| 20090075261 | SYSTEM AND METHOD FOR AUTHENTICATING TABLETS - A method for authenticating and verifying a pharmaceutical item to be genuine is described. The method for authenticating a tablet comprises applying a particular nucleic acid material associated with a particular sequence of nucleic acid bases to a tablet or capsule. The method also comprises collecting a sample of the tablet and verifying the tablet is genuine by detecting the particular nucleic acid material. | 03-19-2009 |
| 20090075262 | Gene Methylation In Endometrial Cancer Diagnosis - The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with endometrial cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use the detection and diagnosis of endometrial cancer. | 03-19-2009 |
| 20090075263 | GENE METHYLATION IN OVARIAN CANCER DIAGNOSIS - The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with ovarian cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use the detection and diagnosis of ovarian cancer. | 03-19-2009 |
| 20090075264 | GENE METHYLATION IN LIVER CANCER DIAGNOSIS - The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with liver cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use the detection and diagnosis of liver cancer. | 03-19-2009 |
| 20090075265 | GENE METHYLATION IN THYROID CANCER DIAGNOSIS - The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with thyroid cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use the detection and diagnosis of thyroid cancer. | 03-19-2009 |
| 20090075266 | MULTIPLE ANALYTE DIAGNOSTIC READOUT - The invention provides methods for assessing clinical status through the creation of a diagnostic readout based upon the analysis of multiple biomarkers. According to the invention, an algorithm is provided that allows the use of multiple biomarker thresholds from a patient sample to be used in order to increase the sensitivity and specificity of a diagnostic procedure. | 03-19-2009 |
| 20090075267 | K-ras and B-raf mutations and anti-EGFr antibody therapy - The present application relates to K-ras mutations, to polynucleotides encoding mutant K-ras polypeptides, and to methods of identifying K-ras mutations. The present application also relates to B-raf mutations, to polynucleotides encoding mutant B-raf polypeptides, to vectors containing those polynucleotides, and to methods of identifying B-raf mutations. The present application also relates to methods of diagnosing cancer; and methods and kits for predicting the usefulness of anti-EGFr specific binding agents in the treatment of tumors. | 03-19-2009 |
| 20090075268 | Antigenic Targets Of Autoimmune Sensorineural Hearing Loss (AISNHL) And Development Of Tests For Diagnosis And Management Of AISNHL - This invention relates generally to antigens reactive with autoantibodies associated with autoimmune sensorineural hearing loss (AISNHL), and in particular relates to a novel antigen, Inner Ear Supporting Cell Antigen (IESCA) reactive with autoantibodies associated with autoimmune sensorineural hearing loss (AISNHL), and to methods for the detecting therapeutic outcomes in treatment of AISNHL patients, and to methods of detecting genetic lesions in genes encoding for such antigens. | 03-19-2009 |
| 20090075269 | SYSTEM AND METHOD FOR HIGH RESOLUTION ANALYSIS OF NUCLEIC ACIDS TO DETECT SEQUENCE VARIATIONS - Provided herein are methods for assaying a biological sample for microorganisms having drug resistant and/or drug sensitive phenotypes, wherein the methods are capable of detecting resistant and sensitive phenotypes associated with known and/or unknown mutations. In some aspects, methods are provided for detecting drug resistant | 03-19-2009 |
| 20090075270 | Products and Methods Relating to the Use of the Endoribonuclease Kid/PemK - The invention relates to a method for engineering a nucleic acid for expression in the presence of Kid/PemK endoribonuclease comprising (i) screening the nucleotide sequence of the nucleic acid for the sequence UUACU or TTACT (ii) mutating said sequence such that there are no longer any occurrences of UUACU or TTACT. The invention also relates to a method of making a ribonucleic acid resistant to Kid/PemK endoribonuclease, said method comprising (a) providing a nucleic acid; (b) screening the nucleic acid for the nucleotide sequence UUACU or TTACT; (c) mutating said sequence such that there are no longer any occurrences of UUACU or TTACT; wherein when the nucleic acid of (a) is a deoxyribonucleic acid, said method further comprises (d) transcribing said deoxyribonucleic acid to produce ribonucleic acid. The invention also relates to vectors and uses of purified or recombinant Kid/PemK endoribonucleases. | 03-19-2009 |
| 20090075271 | Methods of identifying individuals at risk of perioperative bleeding, renal dysfunction or stroke - The present invention relates, in general, to perioperative bleeding and, in particular, to methods of identifying individuals at risk of perioperative bleeding. | 03-19-2009 |
| 20090075272 | Method to Identify CD40-Sensitive Cells Using Gene Expression - Gene expression patterns were analyzed in CD40-sensitive and CD40-resistant diffuse large-cell B-lymphoma (DLCBL) cell lines to identify signaling pathways which are involved in CD40-mediated apoptosis. CD40-resistant lines expressed pre-B cell markers including RAG and VPREB, whereas CD40-sensitive cells resembled mature B-cells and expressed higher levels of transcripts encoding several members of the CD40 signaling pathway including LCK and VAV. In addition, CD40 sensitive DLCBL cell lines also displayed constitutive activation of ERK and failed to undergo apoptosis when ERK phosphorylation was inhibited. In contrast, CD40 resistant lines showed no constitutive activation of ERK and no increase in ERK activity in response to CD40 stimulation. The invention includes methods to differentiate between CD40-sensitive and CD-40 resistant cells based on these differences in gene expression. | 03-19-2009 |
| 20090075273 | Planar Waveguide Detection Chips and Chambers for Performing Multiple PCR Assays - Provided are planar waveguide (“PWG”) detection chips that are used to perform multiplex PCR and kinetic PCR assays with a single fluorescent dye. The PWG detection chips are housed in PWG detection chambers that house at least one PWG chip. The PWG detection chambers may be in a single chamber or a dual chamber configuration. Also provided are methods for analyzing amplification products using the PWG detection chambers of the present invention. | 03-19-2009 |
| 20090075274 | MULTIPLEXED QUANTITATIVE DETECTION OF PATHOGENS - The invention allows for the quantitative detection of a plurality of pathogens in a single sample. The method includes the amplification of a sample with a plurality of pathogen-specific primer pairs to generate amplicons of distinct sizes from each of the pathogen specific primer pairs. The method further includes the use of a plurality of competitor polynucleotide targets that correspond to each of the pathogen-specific primer pairs. The competitor polynucleotides are added to the reaction mixture at a known concentration to allow for the quantitation of the amount of pathogen in the sample. The method can be used for monitoring pathogen infection in an individual, preferably an immunocompromised individual. | 03-19-2009 |
| 20090075275 | NUCLEIC ACID PROBE-IMMOBILIZED SUBSTRATE AND METHOD OF DETECTING THE PRESENCE OF TARGET NUCLEIC ACID BY USING THE SAME - The invention provides a nucleic acid probe-immobilized substrate comprising a substrate and a nucleic acid probe containing a nucleotide sequence complementary to a target sequence and immobilized via a spacer onto the substrate, wherein upon hybridization, with the nucleic acid probe, of a target nucleic acid partially containing the target sequence, the spacer satisfies the relationship: | 03-19-2009 |
| 20090075276 | High throughput mutation screening methods and kits using a universalized approach - differential sequence fill-in (dsf)-enabled sequential adapter ligation and amplification - This disclosure teaches high throughput mutation screening methods allowing simultaneous analysis of multiple genetic regions of interest and sensitive detection of very low frequency mutation(s) by the use of a universalized approach. Methods comprise treating RNA:DNA heteroduplexes of interest with a ribonuclease, sequence extension by an RNA-primed DNA polymerase, ligation with a blocking adapter, and differential sequence fill-in followed by single-strand-specific nuclease digestion to permit full-length sequence extension and subsequent ligation with a tagged reporter adapter solely in mutants filled in with a complementary deoxyribonucleotide triphosphate. By forming tagged mutant-dual adapter hybrids or mutant-triple adapter hybrids, the detection and/or quantification of mutants may be directed to the commonly shared tag(s) or flanking adapter sequences for signal detection/enhancement or sequence amplification in all different mutants regardless of the source or the number of mutations involved, thereby avoiding the tremendous effort of multiple target-specific sequence amplifications. Methods may be performed wholly or partially in solution, on solid phase media, in large scale, adapted for automated or semi-automated analysis, and any combinations thereof. | 03-19-2009 |
| 20090075277 | Method for detecting ovarian cancer and method for suppressing the same - ABSTRACT An object of the present invention is to provide a method for detecting cancer through identification of genes exhibiting characteristic behavior in the cases of cancer such as ovarian cancer, and a cell growth inhibitor. The present invention provides a method for detecting cancer, which comprises detecting canceration including malignancy of a specimen through detection of at least one alteration of a gene existing in a chromosomal region 2q14. 2, 3p24. 1, 3q26. 2, 3q29, 4q34. 2, 6q23, 9p21. 3, 11q13. 3, 13q22.1, 13q33. 1, 13q33. 3, 15q12, 15q15. 1, 17p12, 17p13. 1, 17p13. 3, 18q21. 1, 18q21. 2, 18q21. 31, 18q21. 32, 18q21. 33, 18q23, 20q13. 13, 20q13. 2, 20q13. 31, 20q13. 33, Xp11. 23, Xp13.1, Xp13. 3, Xp26. 2, Xp26. 3, or Xq28 in the specimen. | 03-19-2009 |
| 20090075278 | Compositions and methods for the diagnosis and treatment of tumor - The present invention is directed to compositions of matter useful for the diagnosis and treatment of tumor in mammals and to methods of using those compositions of matter for the same. | 03-19-2009 |
| 20090075279 | Compositions and methods for the diagnosis and treatment of tumor - The present invention is directed to compositions of matter useful for the diagnosis and treatment of tumor in mammals and to methods of using those compositions of matter for the same. | 03-19-2009 |
| 20090075280 | EUBACTERIAL tmRNA SEQUENCES AND USES THEREOF - The present invention is directed to eubacterial tmDNA sequences and the corresponding tmRNA sequences. The present invention is further directed to alignments of eubacterial tmDNA sequences and the use of the sequences and sequence alignments for the development of antibacterial drugs. The present invention is also directed to the use of the sequences for the development of diagnostic assays. | 03-19-2009 |
| 20090075281 | MTBE GENES - The present invention provides methods and compositions for modulating MTBE degradation. The invention also provides methods for identifying compounds that modulate MTBE expression. | 03-19-2009 |
| 20090075282 | ARTIFICIAL TISSUE CONSTRUCTS COMPRISING ALVEOLAR CELLS AND METHODS FOR USING THE SAME - The present invention comprises artificial tissue constructs that serve as in vitro models of mammalian lung tissue. The artificial tissue constructs of the present invention comprise functionally equivalent in vitro tissue scaffolds that enable immunophysiological function of the lung. The constructs can serve as novel platforms for the study of lung diseases (e.g., interstitial lung diseases, fibrosis, influenza, RSV) as well as smoke- and smoking-related diseases. The artificial tissue constructs of the present invention comprise the two components of alveolar tissue, epithelial and endothelial cell layers. | 03-19-2009 |
| 20090075283 | Vectors and Methods for Cloning Gene Clusters or Portions Thereof - The present invention relates to a shuttle BAC vector for facilitating the cloning, transfer and heterologous expression of streptomycete secondary metabolite biosynthetic gene clusters. The invention also relates to a plasmid rescue method using this vector for enhancing the process of cloning biosynthetic gene clusters for secondary metabolites from streptomycetes without sophisticated generation and screening of cosmids or BAC libraries. The cloned DNA can then be used for sequencing or heterologous expression of putative secondary metabolic gene clusters. | 03-19-2009 |
| 20090075284 | METABOLOMIC PROFILING OF PROSTATE CANCER - The present invention relates to cancer markers. In particular, the present invention provides metabolites that are differentially present in prostate cancer. | 03-19-2009 |
| 20090075285 | PRIMER KIT - The aim of the present invention is to provide a primer kit capable of efficiently amplifying only a required target nucleotide sequence without shortage or excess in the case of amplifying a wide range of target nucleotide sequences. The present invention is directed to a primer kit having a plurality of types of primers for amplifying nucleic acids having a plurality of types of target nucleotide sequences, wherein all of the types of primers are primers capable of amplifying each nucleic acid having a target nucleotide sequence under the same reaction conditions, and a single type of primer or primers corresponding to a single target nucleotide sequence is/are housed in at least one container; and a primer kit having a plurality of types of primers for amplifying nucleic acids having a plurality of types of target nucleotide sequences, wherein all of the types of primers are primers capable of amplifying each nucleic acid having a target nucleotide sequence under the same reaction conditions, and primers corresponding to a single target nucleotide sequence are housed in the same container. | 03-19-2009 |
| 20090075286 | Detection of Polyketide Synthetase Gene Expression in Karenia Brevis - The present invention concerns an assay for the detection of polyketide synthetase (PKS) mRNA from the red tide dinoflagellate | 03-19-2009 |
| 20090075287 | METHODS FOR IDENTIFYING NOVEL PESTICIDAL GENE HOMOLOGUES - Methods and compositions for identifying novel pesticidal gene homologues are provided. Specifically, the methods of the invention comprise systematically designing oligonucleotide primers that are specific for a pesticidal gene of interest and performing successive rounds of PCR amplification of nucleic acid material from a microorganism, particularly a | 03-19-2009 |
| 20090075288 | METHOD FOR DE NOVO DETECTION OF SEQUENCES IN NUCLEIC ACIDS: TARGET SEQUENCING BY FRAGMENTATION - The present invention provides a method for determining nucleic acid sequences of a template nucleic acid that requires no prior knowledge of the nucleic acid sequence present in the template nucleic acid. The method is based on combining information about the mass of a fragment, the mass of any one nucleotide and the combinations thereof, and the sequence specificity of a nucleotide cutter, either enzymatic or chemical cutter, to determine a sequence of a nucleic acid fragment. This method allows for de novo detection of sequences in a target nucleic acid without requiring any prior sequence information. This method is called Partial Sequencing by Fragmentation (PSBF) and it works by fragmenting a target into oligo- or polynucleotides whose masses or lengths are uniquely associated with known sequences. The identities of these sequences are determined solely by the specific fragmentation method used, and are always independent of the target. PSBF can be implemented using electrophoresis, mass spectrometry or any other method that can be used to distinguish the size of the cut nucleic acid sequence fragments. | 03-19-2009 |
| 20090075289 | Collection Assembly for Obtaining Oral Samples From an Animal - The present invention provides a collection assembly for obtaining a sample from within the mouth of an animal. The collection assembly comprises a collection wand, having a scoop-shaped collecting end, and, optionally, an oral speculum. Also provided are methods of using the collection assembly to obtain an oral sample from the mouth of an animal without causing significant discomfort to the animal. | 03-19-2009 |
| 20090081642 | Novel apoptosis gene EI24, compositions, and methods of use - Disclosed is the isolation and characterization of EI24, a novel gene whose 2.4 kb mRNA is induced following etoposide treatment. Induction of EI24 mRNA by etoposide required expression of wild-type p53. Overexpression of functional p53 was sufficient to induce expression of the EI24 mRNA. The EI24 mRNA was also induced in a p53-dependent manner by ionizing irradiation of primary murine thymocytes. The invention is thus directed to an isolated EI24 protein, nucleotide sequences coding for and regulating expression of the protein, antibodies directed against the protein, and recombinant vectors and host cells containing the genetic sequences coding for and regulating the expression of the protein sequence. The invention is also directed to genomic DNA, cDNA, and RNA encoding the EI24 protein sequence and to corresponding antisense RNA sequences. Antibodies can be used to detect EI24 in biological specimens, including, for example, human tissue samples. The present invention is further directed to methods of treating degenerative disorders characterized in inappropriate cell proliferation or inappropriate cell death. The present invention is further directed to methods for diagnosing degenerative disorders characterized in inappropriate cell proliferation or inappropriate cell death, as well as methods for monitoring the progress of such degenerative disorders. | 03-26-2009 |
| 20090081643 | METHODS FOR EVALUATING RIBONUCLEOTIDE SEQUENCES - Methods for identifying ribonucleotide sequences, in vitro, using the ribosome-mediated translation, are provided. | 03-26-2009 |
| 20090081644 | LIGATION AMPLIFICATION - Provided herein are methods and agents for ligation-based exponential ribonucleic acid amplification followed by detection using a nucleic acid polymerization reaction employing terminal-phosphate-labeled nucleotides including three or more phosphates as substrates for nucleic acid polymerase. | 03-26-2009 |
| 20090081645 | METHOD OF ASSUMING DRUG SENSITIVITY TO CDK4 INHIBITOR - It is intended to provide a gene for assuming drug sensitivity, which is to be used for assuming the drug sensitivity to a CDK4 inhibitor based on the expression amount in a test tissue or a test cell, selected from the group consisting of p16 gene, p18 gene and p27 gene. It is also intended to provide a method of assuming the drug sensitivity to a CDK4 inhibitor by using the expression amount of the above gene in a test tissue or a test cell as an indication. | 03-26-2009 |
| 20090081646 | Universal primers and their use for detecting and identifying plant materials in complex mixtures - The invention relates to polynucleotides and primers flanking a variable region of the intron of the chloroplast gene trnL of plant materials for detecting and identifying plant species. The invention also relates to methods for detecting and identifying plant species in complex or degraded mixtures. | 03-26-2009 |
| 20090081647 | Detection of a genetic predisposition to cancers and non-cancerous pathologies in mammals - Methods are provided for the detection of susceptibility to cancer and non-cancerous pathologies in a mammal. Diagnostic kits are also provided. | 03-26-2009 |
| 20090081648 | Detection and analysis of influenza virus - An assay comprising more than one primer pair and more than one detection probe, a low copy number synthetic amplicon corresponding to each of the primer pairs. The assay can detect and distinguish between various sub-types and strains of an influenza virus using any suitable nucleic acid amplification technique. Related kits and methods are also described. | 03-26-2009 |
| 20090081649 | Diagnostic method for detecting cancer by measuring amount of cytokine like il -6 - The present invention relates to a diagnostic method for predicting the possible recurrence of tumours in cancer patients. The method comprises culturing blood cells from a patient suffering from cancer in the presence of a cytokine stimulating factor, where after the amount of induced cytokine thereby produced is determined giving an indication of the risk of recurrence of the cancer. | 03-26-2009 |
| 20090081650 | Method for Identifying Nucleotide Sequences, Use of the Method and Test Kit - A method is disclosed for identifying nucleotide sequences while using non-labeled free oligonucleotides, labeled free and hybridizable oligonucleotides and non-labeled and immobilized oligonucleotides. | 03-26-2009 |
| 20090081651 | METHODS FOR ISOLATING AND CHARACTERIZING ENDOGENOUS mRNA-PROTEIN (mRNP) COMPLEXES - Cellular mRNA-protein (mRNP) complexes are partitioned in vivo by contacting a biological sample with at least one ligand that specifically binds at least one component of a mRNP complex. Suitable biological samples comprise at least one mRNA-protein (mRNP) complex and include cell cultures, cell extracts, and whole tissue, including tumor tissue. Ligands include antibodies that specifically bind RNA-binding or RNA-associated proteins present in the mRNP complex. The mRNP complex is separated by binding the ligand with a binding molecule specific for the ligand, where the binding molecule is attached to a solid support. The mRNP complex is collected by removing the mRNP complex from the solid support. After collecting the mRNP complex, the mRNA bound within the complex may be characterized and identified. Subsets of the total mRNA population of a cell may accordingly be characterized, and a gene expression profile of the cell obtained. | 03-26-2009 |
| 20090081652 | Constitutively Translocating Cell Line - The present invention relates to agonist-independent methods of screening for compounds that alter GPCR desensitization. Included in the present invention are cell lines containing GRKs, in which GPCRs are desensitized in the absence of agonist; the GRKs may be modified. The present invention relates to methods to determine if a GPCR is expressed at the plasma membrane, and if the GPCR has an affinity for arrestin. Modified GPCRs which have increased arrestin affinity are included in the present invention. These modified GPCRs are useful in methods to screen for compounds that alter desensitization, including both the agonist-independent methods and agonist-dependent methods described herein. | 03-26-2009 |
| 20090081653 | Insulin-responsive DNA binding proteins-1 and methods to regulate insulin-responsive genes - The present invention relates to the novel protein Insulin-Responsive DNA Binding Protein-1 (IRDBP-1) and nucleotide sequences that encode it. IRDBP-1 binds to nucleic acid regions of genes that respond when cells are exposed to insulin. IRDBP-1 regulates genes important in mediating the insulin response in mammals and in regulating conditions such as diabetes, obesity, insulin-resistant syndrome and cell proliferative disorders. The present invention provides nucleic acids useful as probes for detecting nucleic acids encoding regions of the IRDBP-1 protein. Within the scope of the present invention are recombinant cells, tissues and animals containing non-naturally occurring recombinant nucleic acid molecules encoding IRDBP-1, including expression vectors, antibodies specific for IRDBP-1, assays for IRDBP-1 polypeptide, and methods relating to all of the foregoing, the development of therapeutic and diagnostic agents that mimic, facilitate or inhibit the action of IRDBP-1, and/or are based on relationships to the structure and action of IRDBP-1. | 03-26-2009 |
| 20090081654 | TRANSFECTION KINETICS AND STRUCTURAL PROMOTERS - The invention features methods of analyzing the kinetics properties of transfection reactions. Also featured are methods for creating structural promoters which are effectively unregulated by enhancers and repressors. The structural promoters are significantly more active than the native promoter sequences upon which they are based. | 03-26-2009 |
| 20090081655 | Multiplex Real-time Quantitative PCR - Disclosed are compositions and methods for analyzing multiple nucleic acids using PCR. | 03-26-2009 |
| 20090081656 | METHOD FOR DETECTING A BACTERIAL PATHOGEN - A method is provided for detecting a bacterial pathogen in a sample. One step of the method includes obtaining a sample and then subjecting the sample to nested PCR. The nested PCR is conducted in the presence of at least two outer oligonucleotide primers complementary to a target nucleotide sequence of the bacterial pathogen so that a first amplified product is produced. The target nucleotide sequence includes at least a portion of a 16S-23S ribosomal RNA sequence. The first amplified product is subjected to the nested PCR in the presence of at least two inner oligonucleotide primers complementary to the nucleotide sequence of the first amplified product so that a second amplified product is obtained. Detection of the second amplified product indicates the presence of the bacterial pathogen in the sample. | 03-26-2009 |
| 20090081657 | Genetic Identification And Validation Of Echinacea Species - A method for identification and validation of | 03-26-2009 |
| 20090081658 | GENEMAP OF THE HUMAN GENES ASSOCIATED WITH CROHN'S DISEASE - The present invention relates to the selection of a set of polymorphism markers for use in genome wide association studies based on linkage disequilibrium mapping. In particular, the invention relates to the fields of pharmacogenomics, diagnostics, patient therapy and the use of genetic haplotype information to predict an individual's susceptibility to Crohn's disease and/or their response to a particular drug or drugs. | 03-26-2009 |
| 20090081659 | Reagents for the detection of protein phosphorylation in carcinoma signaling pathways - The invention discloses 364 novel phosphorylation sites identified in carcinoma, peptides (including AQUA peptides) comprising a phosphorylation site of the invention, antibodies specifically bind to a novel phosphorylation site of the invention, and diagnostic and therapeutic uses of the above. | 03-26-2009 |
| 20090081660 | Compositions and methods for the diagnosis and treatment of tumor - The present invention is directed to compositions of matter useful for the diagnosis and treatment of tumor in mammals and to methods of using those compositions of matter for the same. | 03-26-2009 |
| 20090081661 | Alkyl amines improve detection of components of formaldehyde-fixed biological samples - Alkyl amines act to release formaldehyde cross-linking that occurs in biological samples. Thus, contacting alkyl amines to formaldehyde fixed samples is a useful way to render biological components of the samples, including nucleic acids or proteins, more accessible to detection and characterization. | 03-26-2009 |
| 20090081662 | Homogeneous Luminescence Bioassay - This invention relates to a homogenous bioassay for use in measurement of biological activity, its modulation or analyte concentration of a sample, said bioassay comprising a first group comprising an acceptor, which acceptor is a short lifetime fluorescent compound capable of energy transfer, and a second group comprising a quencher, which quencher is capable of energy transfer from an acceptor. The increase or decrease, respectively, of fluorescence of said acceptor due to the decrease or increase, respectively, of energy transfer from said acceptor to said quencher resulting from lengthening or shortening, respectively, of the distance between said acceptor and quencher is measured. Characteristic for the invention is that the bioassay comprises a further third group comprising a donor for energy transfer to said acceptor, which donor is an up-conversion fluorescent compound, a long-lifetime fluorescent compound or an electrogenerated luminescent compound; and said first group comprises a tag, said third group comprises a binder, and said binder has a high affinity for binding to said tag. The fluorescence of said acceptor is brought about by exciting the donor resulting in energy being transferred from the donor to the acceptor. | 03-26-2009 |
| 20090081663 | METHODS, COMPOSITIONS AND KITS FOR DETECTION AND ANALYSIS OF ANTIBIOTIC-RESISTANT BACTERIA - The present invention relates generally to detection of antibiotic-resistant bacteria in a sample. In particular, the invention provides methods, compositions and kits for detecting and analyzing methicillin-resistant | 03-26-2009 |
| 20090081664 | METHOD FOR DETECTING REACTION PRODUCT OF NUCLEIC ACID SYNTHESIS - A method for detecting the occurrence of nucleic acid syntheses using an enzyme through the use of a generated insoluble substance as an indicator. | 03-26-2009 |
| 20090081665 | METHOD OF DIAGNOSIS/PROGNOSIS OF HUMAN CHRONIC LYMPHOCYTIC LEUKEMIA COMPRISING THE PROFILING OF LPL/ADAM GENES - The present invention provides methods of diagnosis and prognosis of human chronic lymphocytic leukemia (CLL) in a subject a patient in need thereof. The methods of the present invention involve measuring the expression profile of two known genes: LPL and ADAM29; and comparing the ratio of their expression to diagnose the presence of CLL or to prognose the likelihood of developing CLL or the symptoms consistent with CLL. | 03-26-2009 |
| 20090081666 | PROBE SET, PROBE CARRIER, AND METHOD FOR DETERMINING AND IDENTIFYING FUNGUS - It is intended to provide a method for identifying a causative fungus of skin disease. The method includes: simultaneously performing amplification treatments under the same conditions using primers common to plural fungal species; then simultaneously performing hybridization procedures under the same conditions using probes respectively specific to fungi; and determining the presence or absence of each fungus from the hybridization intensity of each probe. | 03-26-2009 |
| 20090081667 | Viral Variants With Altered Susceptibility To Nucleoside Analogs And Uses Thereof - The present invention relates generally to viral variants exhibiting reduced sensitivity to particular agents and/or reduced interactivity with immunological reagents. More particularly, the present invention is directed to hepatitis B virus (HBV) variants exhibiting complete or partial resistance to nucleoside analogs and/or reduced interactivity with antibodies to viral surface components including reduced sensitivity to these antibodies. The present invention further contemplates assays for detecting such viral variants, which assays are useful in monitoring anti-viral therapeutic regimens and in developing new or modified vaccines directed against viral agents and in particular HBV variants. The present invention also contemplates the use of the viral variants to screen for agents capable of inhibiting infection, replication and/or release of the virus. | 03-26-2009 |
| 20090081668 | Glycosyltransferase, nucleic acid encoding the glycosyltransferase and method of testing canceration using the nucleic acid - A tumor marker nucleic acid of the present invention is concerned with a nucleic acid hybridizing under stringent conditions to a nucleotide sequence described in SEQ ID NO: 1 or a complementary nucleotide sequence thereof. A method of testing canceration of the present invention is a method comprising diagnosing a biological sample as being cancerous when the transcription level of the nucleic acid in the biological sample significantly exceeds that in a normal biological sample as a control. The present invention also relates to a β1,3-N-acetyl-D-glucosaminyltransferase protein having an activity of transferring N-acetyl-D-glucosamine from a donor substrate to an acceptor substrate through β1,3-linkage. | 03-26-2009 |
| 20090081669 | Fluorescent Assays Using Orthogonal tRNA - Aminoacyl Synthetase Pairs - An unnatural amino acid comprising a fluorescent moiety is site-specifically incorporated into a protein through the use of an O-tRNA/O-RS pair which is orthogonal to the tRNAs and aminoacyl synthetases of a translation system, thereby allowing the protein to be analyzed. | 03-26-2009 |
| 20090081670 | NICKING AND EXTENSION AMPLIFICATION REACTION FOR THE EXPONENTIAL AMPLIFICATION OF NUCLEIC ACIDS - The invention is in general directed to the rapid exponential amplification of short DNA or RNA sequences at a constant temperature. | 03-26-2009 |
| 20090081671 | METHOD AND APPARATUS FOR JUDGING THE PRESENCE OR ABSENCE OF CANCER CELL - The invention provides a method for judging whether cancer cells are present or not in a sample, comprising a obtaining step of obtaining values related to an expression level of a caner marker gene and a housekeeping gene; a first comparing step of comparing the value related to the expression level of the cancer marker gene with a first threshold value; a normalizing step of normalizing the value related to the expression level of the cancer marker gene based on the value related to the expression level of the housekeeping gene; a second comparing step of comparing the normalized value with a second threshold value; and a judging step of judging whether cancer cells are present or not in the sample based on comparison results obtained in the first and second comparing steps, as well as an apparatus for judging whether cancer cells are present or not. | 03-26-2009 |
| 20090081672 | GENES FROM THE 20Q13 AMPLICON AND THEIR USES - The present invention relates to cDNA sequences from a region of amplification on chromosome 20 associated with disease. The sequences can be used in hybridization methods for the identification of chromosomal abnormalities associated with various diseases. The sequences can also be used for treatment of diseases. | 03-26-2009 |
| 20090081673 | PLATENSIMYCIN BIOSYNTHETIC GENE CLUSTER OF STREPTOMYCES PLATENSIS - The present invention relates to the cloning and sequence of a biosynthetic gene cluster from | 03-26-2009 |
| 20090081674 | Methods for Analysis of PDEF and Survivin as Interconnected Cancer Biomarkers and Targets for Personalized Medicine - Provided are methods for determining whether an individual is a candidate to receive treatment with a DNA methylation inhibitor. The method can be performed a biological sample of cancerous tissue of the individual. Determining that PDEF expression is absent or low and survivin expression is present identifies the individual as a candidate to receive a treatment with a DNA methylation inhibitor. The method also includes communicating the result of identifying an individual as a candidate for receiving a DNA methylation inhibitor to a health care provider | 03-26-2009 |
| 20090081675 | METHODS, COMPOUNDS AND SYSTEMS FOR DETECTING A MICROORGANISM IN A SAMPLE - Methods to identify a set of probe polynucleotides suitable for detecting a set of targets and in particular methods for identification of primers suitable for detection of target microorganisms related polynucleotides, set of polynucleotides and compositions, and related methods and systems for detection and/or identification of microorganisms in a sample. | 03-26-2009 |
| 20090081676 | METHODS OF USING FET LABELED OLIGONUCLEOTIDES THAT INCLUDE A 3'-5' EXONUCLEASE RESISTANT QUENCHER DOMAIN AND COMPOSITIONS FOR PRACTICING THE SAME - Methods and compositions are provided for detecting a primer extension product in a reaction mixture. In the subject methods, a primer extension reaction is conducted in the presence of a polymerase having 3′→5′ exonuclease activity and at least one FET labeled oligonucleotide probe that includes a 3′→5′ exonuclease resistant quencher domain. Also provided are systems and kits for practicing the subject methods. The subject invention finds use in a variety of different applications, and are particularly suited for use in high fidelity PCR based reactions, including SNP detection applications, allelic variation detection applications, and the like. | 03-26-2009 |
| 20090081677 | METHOD FOR GENDER IDENTIFICATION OF EAGLES WITH PROBE-BASED REAL-TIME PCR AND THE SEQUENCES USED FOR GENDER IDENTIFICATION OF EAGLES - A method for gender identification of eagles includes: providing a DNA of an eagle; performing a probe-based real-time PCR using the DNA as a template, a universal primer pair P2/P8 as a primer pair and a first probe and a second probe as probes, wherein the 5′ ends of the first probe and the second probe are labeled with a first fluorescent dye and a second fluorescent dye, respectively, and the first probe is a sequence with about 15-38 nucleotides in length of SEQ ID No. 1 and the second probe is a sequence with about 15-44 nucleotides in length of SEQ ID No. 2; and analyzing a result of the PCR, wherein if the result is positive for both the first and the second fluorescent dye, the eagle is a female, and if the result is positive for only the first fluorescent dye, the eagle is a male. | 03-26-2009 |
| 20090081678 | NUCLEIC ACID ISOLATION IN PRESERVED WHOLE BLOOD - A method for isolating nucleic acids is disclosed, wherein a sample having nucleic acid containing starting material is fixed, lysed, and treated to remove unwanted contaminants. The initial fixing of the sample aids in maintaining the structure and integrity of the isolated DNA and reduces the incidence of end product contaminants and DNA shearing. | 03-26-2009 |
| 20090081679 | Compositions and methods for in vivo SELEX - The invention describes methods for the in vivo selection of oligonucleotides, preferably aptamers, that persist in biological compartments. In one embodiment, the biological compartment comprises at least one tissue which, in a preferred embodiment, is the blood within the circulatory system of a living mammal. The invention also contemplates oligonucleotides, preferably aptamers, selected through in vivo SELEX that may be linked to therapeutic or diagnostic compositions including other oligonucleotides. | 03-26-2009 |
| 20090081680 | Mutant firefly luciferase - This invention relates to: the development of a mutant firefly luciferase in order to use dATP as a DNA polymerase substrate upon pyrosequencing, such luciferase being subjected to substrate specificity modification in a manner such that the dATP-induced activity alone is decreased while the ATP-induced activity is maintained; and a mutant firefly luciferase for which the proportion of activity induced by dATP to activity induced by ATP (dATP/ATP) is lower than that for the wild-type firefly luciferase, in which an amino acid identified based on homology analysis as corresponding with the 421 | 03-26-2009 |
| 20090081681 | Method For Genetic Selection Of High-Plasmid Producing E. Coli Clones - The present invention relates to methods of selecting for highly productive clones of | 03-26-2009 |
| 20090081682 | HIGH-THROUGHPUT METHODS FOR IDENTIFYING GENE FUNCTION USING LENTIVIRAL VECTORS - The present invention relates to methods and compositions for the efficient identification of one or more functionalities of a product encoded by a nucleic acid sequence of interest. The methods utilize the abilities to over and/or under express the product in a cell, as well as the combination of these results, to permit the identification of at least one of the product's cellular or in vivo functionality. | 03-26-2009 |
| 20090081683 | Kits and Methods for Assessing the Coenzyme Q Reducing Status of a Patient, Including a Patient Ingesting a Statin - The disclosure relates to kits and methods for assessing whether an individual is likely to benefit from nutritional supplementation with coenzyme Q and, more particularly, a reduced form of coenzyme Q. The methods involve assessing occurrence of a polymorphism in the gene encoding NQO1 in the individual. Individuals homozygous for the polymorphism will receive optimal benefits from supplementation with the reduced form of coenzyme Q. The disclosure further relates to methods for predicting and assigning a coenzyme Q redox status phenotype based on assessment of an individual's genome for a polymorphism in the gene encoding NQO1. | 03-26-2009 |
| 20090081684 | DETECTION OF NEGATIVELY CHARGED POLYMERS USING WATER-SOLUBLE, CATIONIC, POLYTHIOPHENE DERIVATIVES - Novel methods allowing for the simple optical and electrochemical detection of double-stranded oligonucleotides are disclosed. The methods are rapid, selective and versatile. Advantageously, they do not require any chemical reaction on the probes or on the analytes since they are based on different electrostatic interactions between cationic poly(3-alkoxy-4-methylthiophene) derivatives and single-stranded or double-stranded (hybridized) oligonucleotides. | 03-26-2009 |
| 20090081685 | METHODS AND COMPOSITIONS FOR THE DETECTION OF OVARIAN DISEASE - Methods and compositions for identifying ovarian cancer in a patient sample are provided. The methods of the invention comprise detecting overexpression of at least one biomarker in a body sample, wherein the biomarker is selectively overexpressed in ovarian cancer. In preferred embodiments, the body sample is a serum sample. The biomarkers of the invention include any genes or proteins that are selectively overexpressed in ovarian cancer, including, for example, acute phase reactants, lipoproteins, proteins involved in the regulation of the complement system, regulators of apoptosis, proteins that bind hemoglobin, heme, or iron, cytostructural proteins, enzymes that detoxify metabolic byproducts, growth factors, and hormone transporters. In some aspects of the invention, overexpression of a biomarker of interest is detected at the protein level using biomarker-specific antibodies or at the nucleic acid level using nucleic acid hybridization techniques. Kits for practicing the methods of the invention are further provided. | 03-26-2009 |
| 20090081686 | PHOTOCLEAVABLE LABELED NUCLEOTIDES AND NUCLEOSIDES AND METHODS FOR THEIR USE IN DNA SEQUENCING - Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3′-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format. The design of such rapidly cleavable fluorescent groups on nucleotides and nucleosides can enhance the speed and accuracy of sequencing of large oligomers of DNA in parallel, to allow rapid whole genome sequencing, and the identification of polymorphisms and other valuable genetic information, as well as allowing further manipulation and analysis of nucleic acid molecules in their native state following cleavage of the fluorescent group. | 03-26-2009 |
| 20090081687 | Nucleic Acid Molecules and Other Molecules Associated with Plants - Expressed Sequence Tags (ESTs) isolated from soybean are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts. | 03-26-2009 |
| 20090081688 | Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations - Methods of detecting multiple nucleic acid targets in single cells through indirect capture of labels to the nucleic acids are provided. Methods of assaying the relative levels of nucleic acid targets through normalization to levels of reference nucleic acids are also provided. Methods of detecting individual cells, particularly rare cells from large heterogeneous cell populations, through detection of nucleic acids are described. Related compositions, systems, and kits are also provided. | 03-26-2009 |
| 20090081689 | Reagents and methods to enrich rare cells from body fluids - The present invention relates to compositions and methods for separating cells. The present invention utilizes a combination of techniques to deplete non-nucleated and nucleated cells in a biological sample. The use of this invention assists in reducing the complexity of a biological sample such as peripheral blood, and help in the diagnosis and prognosis of many conditions. The invention includes solutions and methods to selectively deplete red and white blood cells from a blood sample. The preferred sample is blood, effusion, or aspirate samples containing one or more cell types that can be enriched from such a sample. The present invention provides methods for identifying target cells, nucleic acids or chromosome quantification. | 03-26-2009 |
| 20090081690 | Site-specific labeling of proteins for NMR studies - Methods of producing and/or analyzing spectroscopically labeled proteins, e.g., proteins site-specifically labeled with NMR active isotopes, spin-labels, chelators for paramagnetic metals, and the like, are provided. The labeled proteins are produced in translation systems including orthogonal aminoacyl tRNA synthetase/tRNA pairs. Methods for assigning NMR resonances, e.g., methods using isotopically labeled proteins, are also provided. | 03-26-2009 |
| 20090087834 | Method for characterising polynucleotides - The method of the invention is used to identify specific characteristics of a target polynucleotide in a sample, and comprises the steps of: i) attaching to one end of each target polynucleotide in the sample a polynucleotide signal sequence that is specific for the characteristic under study; ii) contacting the target polynucleotides with a molecule that interacts with the target polynucleotide if the characteristic is present; iii) attaching a polynucleotide adapter sequence to those targets polynucleotides that interact with the molecule of step (ii), the adapter being attached at the end of the target polynucleotide opposite that at which the signal sequence is attached; iv) carrying out a polynucleotide amplification reaction on those target polynucleotides that comprise both the adapter and signal sequence, optionally repeating steps (i) to (iv) for other characteristics; and (v) identifying which signal sequences are present on the amplified products, and in which order, to thereby determine the characteristics of each target polynucleotide. | 04-02-2009 |
| 20090087835 | Method of Identifying Hairpin DNA Probes by Partial Fold Analysis - Method of identifying molecular beacons in which a secondary structure prediction algorithm is employed to identify oligonucleotide sequences within a target gene having the requisite hairpin structure. Isolated oligonucleotides, molecular beacons prepared from those oligonucleotides, and their use are also disclosed. | 04-02-2009 |
| 20090087836 | IL-B30 ANTIBODIES - Purified genes encoding cytokine from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this molecule are provided. Methods of using said reagents and diagnostic kits are also provided. | 04-02-2009 |
| 20090087837 | Method Of Detecting Streptococcus Pneumoniae, Primer Set For The Detection And Kit For The Detection - A method of detecting | 04-02-2009 |
| 20090087838 | ANALYTE DETECTION USING AUTOCATALYTIC CHAIN REACTIONS - Compositions and methods for detecting the presence of analytes employing autocatalytic chain reactions (ACR) having super linear kinetics for amplification of signal are disclosed. | 04-02-2009 |
| 20090087839 | LONG DISTANCE POLYMERASE CHAIN REACTION-BASED ASSAY FOR DETECTING CHROMOSOMAL REARRANGEMENTS - Methods are presented for determining the presence of an inversion in the factor VIII gene which cause hemophilia A. The methods encompass long distance, multiplex PCR (including overlapping PCR). The use of deaza-dGTP, high levels of DNA polymerases and high levels of DMSO aid in successfully performing the PCR. The use of a novel technique called subcycling PCR can also be applied as part of the methods. The technique allows for the determination of whether a person is homozygous or hemizygous for the inversion and has hemophilia A or whether a person is heterozygous for the inversion and is a carrier. The technique of long distance, multiplex PCR including use of deaza-dGTP, high levels of DNA polymerases and high levels of DMSO are applicable to the determination of the presence of other gross chromosomal aberrations such as deletions/inversions, translocations and inversions. The use of subcycling PCR can achieve efficient and more even amplification than normal two or three temperature PCR and is applicable to long distance, multiplex PCR. | 04-02-2009 |
| 20090087840 | Combined extension and ligation for nucleic acid assembly - Certain aspects of the present invention provide methods for assembling nucleic acid molecules. Some embodiments involve analyzing nucleic acid sequences and determining appropriate assembly strategies based on the presence or absence of sequence features that are known or predicted to interfere with extension-based and/or ligation-based assembly techniques. Aspects of the invention also provide kits, compositions, devices, and systems for assembling synthetic nucleic acids using polymerase-based techniques, ligase-based techniques, or combinations thereof. | 04-02-2009 |
| 20090087841 | METHODS AND COMPOSITIONS FOR DETERMINING RESISTANCE OF HIV-1 TO PROTEASE INHIBITORS - This invention relates to methods for determining resistance of HIV-I viruses to protease inhibitors (PIs) based on the viral genotypes. The methods generally comprise detecting, in a gene encoding protease of the HIV-I, the presence of a mutation in at least one of codon 22, 69, 74, or 83 alone or in combination with one or more mutations at certain other codons, or, in a gene encoding gag of the HIV-I, the present of a mutation in at least one of codon 418 or 482 alone or in combination with one or more mutations at certain other codons. Combinations of mutations associated with resistance to PIs are also disclosed. | 04-02-2009 |
| 20090087842 | Presynaptic protein cast - The present invention enabled the detection and quantification of CAST, which is localized to synapses and tightly bound to the cytomatrix, and of the mRNA encoding the CAST. Furthermore, it was revealed that CAST functions as a protein scaffold for localizing RIM1 to synapses, contributing as a molecular basis for active zone formation. | 04-02-2009 |
| 20090087843 | MOLECULAR MARKERS - The present invention relates to genetic sequences exhibiting differential expression patterns in cancer tissue relative to normal tissue. The identification of such sequences permits their use as molecular markers for cancer, as early indicators of cancer progression and/or as predictive markers for a propensity or likelihood of a cancer to develop. The present invention relates particularly to genetic sequences exhibiting expression patterns up-regulated in cervical cells or associated with pre-, early- or late-onset cervical cancer relative to normal cervical cells, such sequences serving as biomarkers. The biomarkers of the present invention provide targets for the development of therapeutic protocols for the treatment or prophylaxis of cervical or related cancer. Such therapeutic protocols are directed to inhibiting expression of the marker or inhibiting the expression product of the marker. The invention is further directed to a method for identifying molecular markers which are useful indicators of cervical cancer and/or its progression. | 04-02-2009 |
| 20090087844 | METHODS AND COMPOSITIONS FOR CORRELATING GENETIC MARKERS WITH CARDIOVASCULAR DISEASE - The present invention provides methods of identifying a subject having an increased or decreased risk of developing cardiovascular disease, comprising:
| 04-02-2009 |
| 20090087845 | Genetic Markers Of True Low Birth Weight - The present application provides for a method of diagnosing TLBW via measurement of expression of various TLBW-related genes. The present application also provides kits for the diagnosis of TLBW in a subject. The present application also provides a method for determining the basis for appropriate therapy for a subject suffering from TLBW. | 04-02-2009 |
| 20090087846 | Method for Detecting Large Mutations and Duplications Using Control Amplification Comparisons to Paralogous Genes - Methods for querying biological samples to detect genetic mutations, particularly insertions and deletions, by co-amplification of a gene of interest in conjunction with a paralogous gene. When the gene of interest and the corresponding paralogous gene are selected from the CYP450 family, the resulting ratios may predict how a particular patient metabolizes certain prescription drugs. | 04-02-2009 |
| 20090087847 | DETERMINING A NUCLEIC ACID SEQUENCE IMBALANCE - Methods, systems, and apparatus are provided for determining whether a nucleic acid sequence imbalance exists within a biological sample. One or more cutoff values for determining an imbalance of, for example, the ratio of the two sequences (or sets of sequences) are chosen. The cutoff value may be determined based at least in part on the percentage of fetal DNA in a sample, such as maternal plasma, containing a background of maternal nucleic acid sequences. The cutoff value may also be determined based on an average concentration of a sequence per reaction. In one aspect, the cutoff value is determined from a proportion of informative wells that are estimated to contain a particular nucleic acid sequence, where the proportion is determined based on the above-mentioned percentage and/or average concentration. The cutoff value may be determined using many different types of methods, such as sequential probability ratio testing (SPRT). | 04-02-2009 |
| 20090087848 | DETERMINING SEGMENTAL ANEUSOMY IN LARGE TARGET ARRAYS USING A COMPUTER SYSTEM - A method and/or system for making determinations regarding samples from biologic sources including statistical methods for making meaning grouping of observed data and/or for pre-selecting endpoints. | 04-02-2009 |
| 20090087849 | NUCLEIC ACID-BASED METHODS AND COMPOSITIONS FOR THE DETECTION OF OVARIAN CANCER - Methods and compositions for identifying ovarian cancer in a patient sample are provided. The methods of the invention comprise detecting overexpression or underexpression of at least one nucleic acid biomarker in a body sample, wherein the biomarker is selectively overexpressed or underexpressed in ovarian cancer. The body sample may be, for example, an ovarian tissue sample. The biomarkers of the invention include any nucleic acid molecule that is selectively overexpressed in ovarian cancer, including, for example, MMP-7, PAEP, CA125, HE4, PLAUR, MUC-1, SLPI, SSP1, MSLN, SPON1, interleukin-7, folate receptor 1, claudin 3, inhibin A, inhibin BB, inhibin BA, and PAI-1. Overexpression or underexpression of a biomarker of interest is detected at the nucleic acid level using such methods as real-time PCR and various nucleic acid hybridization techniques. Kits for practicing the methods of the invention are further provided. | 04-02-2009 |
| 20090087850 | NUCLEIC ACID SEQUENCING METHODS AND SYSTEMS - Sequencing methods that use an exonuclease that comprises template dependent nucleobase binding activity are provided. Related compositions and sequencing systems are also provided. | 04-02-2009 |
| 20090087851 | Lineage-Restricted Neuronal Precursors - A self-renewing restricted stem cell population has been identified in developing (embryonic day 13.5) spinal cords that can differentiate into multiple neuronal phenotypes, but cannot differentiate into glial phenotypes. This neuronal-restricted precursor (NRP) expresses highly polysialated or embryonic neural cell adhesion molecule (E-NCAM) and is morphologically distinct from neuroepithelial stem cells (NEP cells) and spinal glial progenitors derived from embryonic day 10.5 spinal cord. NRP cells self renew over multiple passages in the presence of fibroblast growth factor (FGF) and neurotrophin 3 (NT-3) and express a characteristic subset of neuronal epitopes. When cultured in the presence of RA and the absence of FGF, NRP cells differentiate into GABAergic, glutaminergic, and cholinergic immunoreactive neurons. NRP cells can also be generated from multipotent NEP cells cultured from embryonic day 10.5 neural tubes. Clonal analysis shows that E-NCAM immunoreactive NRP cells arise from an NEP progenitor cell that generates other restricted CNS precursors. The NEP-derived E-NCAM immunoreactive cells undergo self renewal in defined medium and differentiate into multiple neuronal phenotypes in mass and clonal culture. Thus, a direct lineal relationship exists between multipotential NEP cells and more restricted neuronal precursor cells present in vivo at embryonic day 13.5 in the spinal cord. Methods for treating neurological diseases are also disclosed. | 04-02-2009 |
| 20090087852 | MAMMALIAN SELENOPROTEIN DIFFERENTIALLY EXPRESSED IN TUMOR CELLS - A 15 kDa selenium-containing protein (“selenoprotein”) is disclosed. The protein is shown to be differentially expressed in cancer cells, such as prostate cancer cells. There is a correlation between the presence of a polymorphism at nucleotide positions 811 and 1125 of the 15 kDa selenoprotein gene, and the presence of cancer. This polymorphism is more prevalent in the African American population. The determination of an individual's genotype may be used as an indicator of the need for dietary selenium supplementation to inhibit tumor development. Compositions including the isolated protein, specific binding agents that recognize the protein, as well as underlying nucleic acid sequences are presented, as are methods of using such compositions. | 04-02-2009 |
| 20090087853 | HERBICIDE TOLERANT COTTON PLANTS AND METHODS FOR PRODUCING AND IDENTIFYING SAME - The invention pertains to transgenic cotton plants, plant material and seeds, characterized by harboring a specific transformation event, particularly by the presence of a gene encoding a protein that confers herbicide tolerance, at a specific location in the cotton genome. The cotton plants of the invention combine the herbicide tolerant phenotype with optimal agronomic performance. | 04-02-2009 |
| 20090087854 | METHODS FOR GENETIC ANALYSIS - Methods of treating an individual exhibiting a medical condition are disclosed. The methods involve determining a score of an individual based on the individual's genotypic information, comparing the score to at least one threshold value, wherein the result of the comparison is indicative of a beneficial response to a treatment, and providing a suitable treatment to the individual. | 04-02-2009 |
| 20090087855 | MARKERS OF ALTERATIONS IN THE Y CHROMOSOME AND USES THEREFOR - Novel sequence tagged sites (STSs), probes and primers useful, e.g., for detecting the presence or absence of an STS in a sample, and methods of using these STSs, probes and primers, e.g., in methods of detecting alterations in the Y chromosome are disclosed. These compositions are also useful in methods of diagnosing or aiding in the diagnosis and/or cause of reduced sperm count and in methods of predicting or aiding in the prediction of the likelihood of success of infertility treatments. | 04-02-2009 |
| 20090087856 | METHOD FOR ADMINISTERING ANTICOAGULATION THERAPY - The present invention provides a method for use in treating a patient with an anticoagulant to optimize drug therapy and/or to prevent an adverse drug response. More particularly, the present invention relates to a method and system for use in treating a patient with Coumadin® or a substance containing warfarin. Methods of the present invention utilize variables that include the patient's CYP4F2 genotype. | 04-02-2009 |
| 20090087857 | Molecular Beacons for DNA-Photography - The present invention refers to a detection method for analytes using the principle of black-and-white photography and to reagent kits for performing the method, furthermore applied this new technology to detect a biologically relevant sequence in the nanomolar range (femtomoles) in an application circumventing the necessity of a PCR. There are still numerous ways to optimize this methodology that is suitable for a large variety of applications in the genomic diagnostics and proteomics areas. | 04-02-2009 |
| 20090087858 | Two-color Real-time/End-point Quantitation of MicroRNAs (miRNAs) - The present invention is directed to methods, reagents, kits, and compositions for detecting target polynucleotide sequences, especially small target polynucleotides such as miRNAs, between two samples. A pair of linker probes can be employed in two different reactions to query a particular species of target polynucleotide. A pair of detector probes, a single forward primer specific for the target polynucleotide, and a reverse primer can be employed in an amplification reaction to query the difference in expression level of the target polynucleotide between the two samples. In some embodiments a plurality of small miRNAs are queried with a plurality of linker probes. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions. | 04-02-2009 |
| 20090092966 | COLLOIDAL METAL AGGREGATES AND METHODS OF USE - Metal colloidal aggregates substrates useful for metal enhanced fluorescence applications, are disclosed. Method of making and using these colloidal aggregates for enhancing the fluorescent signal in biological assays are also described. | 04-09-2009 |
| 20090092967 | METHOD FOR GENERATING TARGET NUCLEIC ACID SEQUENCES - The present invention provides methods of generating target nucleic acids for amplification using nicking enzymes and methods for amplifying the generated target nucleic acids. | 04-09-2009 |
| 20090092968 | SCREENING METHOD FOR MYCOBACTERIA IN METAL REMOVAL FLUID SYSTEMS - One embodiment of the invention includes a method for testing a fluid for contamination by a mycobacterial organism. A plurality of individual standardized samples may be acquired from a plurality of predetermined sampling points and pooled into a pooled sample. The pooled sample may be analyzed using a polymerase chain reaction technique and the results compared against a pool concern level which is calculated from an individual concern level that governs each individual standardized sample. | 04-09-2009 |
| 20090092969 | DETECTION OF ATYPICAL PNEUMONIA - Disclosed herein are methods and compositions for detecting one or more pathogens that cause atypical pneumonia. Detectable pathogens include | 04-09-2009 |
| 20090092970 | COMPOSITION AND METHOD FOR NUCLEIC ACID SEQUENCING - The present invention provides compositions and methods for detecting incorporation of a labeled nucleotide triphosphate onto the growing end of a primer nucleic acid molecule. The method is used, for example, to genotype and sequence a nucleic acid. In a preferred embodiment, the method described herein detects individual NTP molecules. | 04-09-2009 |
| 20090092971 | Method for Detecting p53 Dysfunction, Method for Molecular Diagnosis of Cancer and Method for Evaluating Compound Effective in Treating Cancer - The present invention relates to methods for detecting a dysfunctional cancer suppressing gene, particularly p53. In other aspects, the invention provides methods for diagnosing cell proliferative disorders such as cancer by measuring expression of a predetermined target gene. Methods of evaluating the efficacy of a test compound to treat a cell proliferative disorder are also included. Such methods generally rely on the expression levels of a predetermined or targeted gene. | 04-09-2009 |
| 20090092972 | Method of evaluating drug sensitivity by analyzing girk channel genes - A method of evaluating drug sensitivity or disease vulnerability, includes linking a gene polymorphism in a GIRK channel gene or a haplotype comprising the gene polymorphism to individual drug sensitivity or individual disease vulnerability. | 04-09-2009 |
| 20090092973 | Grading of Breast Cancer - Methods and compositions for the identification of breast cancer grade signatures are provided. The signature profiles are identified based upon multiple sampling of reference breast tissue samples from independent cases of breast cancer and provide a reliable set of molecular criteria for identification of cells as being in one or more particular stages and/or grades of breast cancer. | 04-09-2009 |
| 20090092974 | Micrornas differentially expressed in leukemia and uses thereof - The present invention concerns methods and compositions for identifying a miRNA profile for acute myeloid leukemia (AML), and using the profile in assessing the condition of a patient. | 04-09-2009 |
| 20090092975 | SELECTABLE MARKER - A nucleic acid sequence encoding a decarboxylation enzyme E.G. PAD1 is used as a selectable marker in a recombinant organism. A weak acid is used as the selecting agent. | 04-09-2009 |
| 20090092976 | Hypermethylation of GATA-4 and GATA-5 Transcription Factor Genes in Cancer - Methods are provided for identifying the presence of cancer cells in a sample by detecting hypermethylation of the promoter region of a GATA-4 transcription factor gene, a GATA-5 transcription factor gene, or both. Methods for ameliorating a cancer by effecting expression of a hypermethylation silenced GATA-4 and/or GATA-5 transcription also are provided. | 04-09-2009 |
| 20090092977 | MASS SPECTROMETRIC METHODS FOR DETECTING MUTATIONS IN A TARGET NUCLEIC ACID - Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and mutations in the molecules are provided. | 04-09-2009 |
| 20090092978 | ASSOCIATION OF UQCRC1SNPs WITH FAT DEPOSITION AND FATTY ACID COMPOSITION - The present invention relates to the identification of single nucleotide polymorphisms (SNPs) in a ubiquinol-cytochrome c reductase core protein I (UQCRC1) gene and its associations association with fat deposition and fatty acid composition. The invention further encompasses methods and systems, including network-based processes, to manage the SNP data, haplotype data and other data relating to specific animals and herds of animals, veterinarian care, diagnostic and quality control data and management of livestock which, based on genotyping, have predictable meat quality traits, husbandry conditions, animal welfare, food safety information, audit of existing processes and data from field locations. | 04-09-2009 |
| 20090092979 | METHODS FOR ISOLATING LONG FRAGMENT RNA FROM FIXED SAMPLES - The present invention relates to methods for the extraction of long fragment RNA from fixed tissue specimens. In particular, the present invention relates to methods for the extraction of RNA from formalin-fixed paraffin-embedded tissue specimens for use in biologic applications, including assays based on oligonucleotide hybridization. | 04-09-2009 |
| 20090092980 | miRNA PROCESSING INHIBITOR EFFICACY ASSAYS AND SUBSTANCES - The invention relates to assays for assessing miRNA maturation effector (preferably: inhibitor) efficacy, and to substances useful for influencing, particularly for inhibiting, maturation of miRNA. According to the invention there is provided assay of miRNA processing inhibitor efficacy, comprising the steps of: a) providing a target miRNA precursor, b) providing a potential inhibitor of one or more processing steps of the target miRNA precursor, c) bringing together of the target miRNA precursor and the potential inhibitor under miRNA maturation conditions, and d) determining inhibition efficiency. The assay of the present invention allows for a very fast and easy assessment of the efficacy of a potential inhibitor in inhibiting processing of a miRNA precursor into miRNA. | 04-09-2009 |
| 20090092981 | TETRAHYDROPYRAN NUCLEIC ACID ANALOGS - The present disclosure describes tetrahydropyran nucleoside analogs, oligomeric compounds prepared therefrom and methods of using the oligomeric compounds. More particularly, tetrahydropyran nucleoside analogs are provided, having one or more chiral substituents, that are useful for enhancing properties of oligomeric compounds including nuclease resistance and binding affinity. In some embodiments, the oligomeric compounds provided herein hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. | 04-09-2009 |
| 20090092982 | METHODS AND COMPOSITIONS FOR INCREASING BIOLOGICAL MOLECULE STABILITY - The present invention relates to methods and compositions for enhancing folding and stability of biological molecules. In particular, the present invention relates to methods and compositions for identifying biological molecules with enhanced stability. The present invention further relates to host cells that confer enhanced stability to biological molecules expressed therein. | 04-09-2009 |
| 20090092983 | IDENTIFICATION OF AN ERBB2 GENE EXPRESSION SIGNATURE IN BREAST CANCERS - A method for analyzing differential gene expression associated with breast tumor is based on the analysis of the over-expression or under-expression of polynucleotide sequences in a biological sample. The analysis includes the detection of the over-expression of at least one polynucleotide sequence(s), subsequence(s) or complement(s) thereof selected from predefined polynucleotide sequence sets. | 04-09-2009 |
| 20090092984 | Determining a predisposition to cancer - The present invention relates to methods and kits for determining a predisposition and surveillance protocols for developing cancer of various sites due to specific mutation in at least one allele of CHEK2 gene and/or at least one allele of NOD2 gene and/or at least one allele of CDKN2A gene. | 04-09-2009 |
| 20090092985 | Screening tools for discovery of novel anabolic agents - The disclosure provides nucleic acids including the polynucleotide sequence of the human MAFbx core promoter involved in muscle specific expression. Also provided are reporters operably linked to a polynucleotide sequence including MAFbx transcription regulatory sequences, and constructs including polynucleotides that encode reporters and other polynucleotide sequences operably linked to the MAFbx core transcription regulatory sequence. Systems for identifying agents that inhibit muscle loss and/or increase muscle mass or tone are also provided. | 04-09-2009 |
| 20090092986 | Fluorescent Nucleobase Conjugates Having Anionic Linkers - Provided are nucleotide-dye conjugates and related compounds in which a dye is linked to a nucleobase directly or indirectly by an anionic linker. The anionic character of the linker is provided by one or more anionic moieties which are present in the linker, such as phosphate, phosphonate, sulfonate, and carboxylate groups. When the dye is a provided as a donor/acceptor dye pair, the anionic linker can be located between the donor and the acceptor, or between the nucleobase and either the donor or acceptor, or both. In one embodiment, conjugates of the invention provide enhanced electrophoretic mobility characteristics to sequencing fragments, e.g., for dideoxy sequencing using labeled terminators. | 04-09-2009 |
| 20090092987 | Polymorphic Nucleic Acids Associated With Colorectal Cancer And Uses Thereof - The present invention provides compositions and methods for research, diagnostic, drug screening, and therapeutic applications related to colorectal cancer and related conditions. In particular, the present invention provides genetic variations in or associated with one or more of the STK38L, GPR45, TGFBRAP1, PADI3, IBRDC1, and/or GRK5 genes as being associated with such conditions. | 04-09-2009 |
| 20090092988 | Modulating Gene Expression with agRNA and Gapmers Targeting Antisense Transcripts - Gene expression is selectively modulated in the genome of a mammalian cell determined to be in need thereof by determining the presence of an encoded antisense transcript overlapping a promoter of the target gene; contacting the transcript with an agRNA or gapmer complementary to a portion of the transcript upstream relative to the transcription start site of the gene; and detecting a resultant modulation of expression of the target gene. | 04-09-2009 |
| 20090092989 | MICROFLUIDIC PLATFORMS FOR MULTI-TARGET DETECTION - Disclosed are example methods and devices for detecting one or more targets. An example method includes placing a sample including a first target with in a microfluidic device and hybridizing a plurality of copies of the first target with a plurality of nanostructures. The example method includes applying an electric current to the plurality of nanostructures and using an electric field created by the electric current to move the plurality of nanostructures. In addition, the plurality of nanostructures are sorted and evaluated to determine at least one of a presence, an absence, or a quantity of the first target. | 04-09-2009 |
| 20090092990 | PRIMERS AND METHODS FOR THE DETECTION AND DISCRIMINATION OF NUCLEIC ACIDS - The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR. | 04-09-2009 |
| 20090092991 | Assays, methods and systems for predicting follicular lymphoma outcome - Assays, kits, methods and systems for predicting outcome in patients with follicular lymphoma based upon measurement of one or more phenomenologically competitive or synergistic gene pairs or a set of classifier genes are provided. | 04-09-2009 |
| 20090092992 | Novel human dickkopf-related protein and nucleic molecules and uses therefor - Novel Dkk and Dkk-related polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length Dkk and Dkk-related proteins, the invention further provides isolated fusion proteins, antigenic peptides and antibodies. The invention also provides Dkk and Dkk-related nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals in which a Dkk and Dkk-related gene has been introduced or disrupted. Diagnostic, screening and therapeutic methods utilizing compositions of the invention are also provided. | 04-09-2009 |
| 20090092993 | Cellular arrays and methods of detecting and using genetic disorder markers - A method is disclosed for rapid molecular profiling of tissue or other cellular specimens by placing a donor specimen in an assigned location in a recipient array, providing copies of the array, and performing a different biological analysis of each copy. The results of the different biological analyses are compared to determine if there are correlations between the results of the different biological analyses at each assigned location. In some embodiments, the specimens may be tissue specimens from different tumors, which are subjected to multiple parallel molecular (including genetic and immunological) analyses. The results of the parallel analyses are then used to detect common molecular characteristics of the genetic disorder type, which can subsequently be used in the diagnosis or treatment of the disease. The biological characteristics of the tissue can be correlated with clinical or other information, to detect characteristics associated with the tissue, such as susceptibility or resistance to particular types of drug treatment. Other examples of suitable tissues which can be placed in the matrix include tissue from transgenic or model organisms, or cellular suspensions (such as cytological preparations or specimens of liquid malignancies or cell lines). | 04-09-2009 |
| 20090092994 | Reagents, methods and kits for classification of fungi and direction of anti-fungal therapy - Provided herein are methods, kits and compositions to classify fungi. Methods are provided for classification of fungi according to established phenotypes, for example, antimicrobial susceptibility profiles. More specifically, the invention provides methods for the use of PNA probes in diagnostic applications, which will aid in the direction of appropriate therapy against fungi. | 04-09-2009 |
| 20090092995 | Method for Identifying Within a Mammal a DNA encoding a Physiologically Active Polypeptide - The present inventors found that a physiological effect of a particular DNA can be detected independently within mice into which a pool of various DNAs in various quantities has been introduced. This finding suggests that it is possible to identify a DNA having a particular physiological effect by successively fractionating a pool of various DNAs in various quantities using the particular physiological effect seen within a mammal as an index. Such a method of screening will have the advantage of saving much time and effort as required in conventional screenings such as those utilizing transgenic and knockout mice. Furthermore, the method of screening has the additional advantage of enabling the identification of a DNA having a physiological activity, for example, even when the cells producing a physiologically active substance cannot be maintained in vitro or in immunodeficient animals, or when the cells change their characteristics during passage and stop producing the physiologically active substance. | 04-09-2009 |
| 20090098533 | Methods and kits for investigating cancer - The invention provides novel compositions, methods and uses, for the prediction, diagnosis, prognosis, prevention and treatment of malignant neoplasia and breast cancer. The invention further relates to genes that are differentially expressed in breast tissue of breast cancer patients versus those of normal “healthy” tissue. Differentially expressed genes for the identification of patients which are likely to respond to chemotherapy are also provided. | 04-16-2009 |
| 20090098534 | Full Karyotype Single Cell Chromosome Analysis - A full set of 24 chromosome-specific probes to analyze single cells or cell organelles to test for abnormalities is described. When used in an assay based on sequential hybridization, the full set is comprised of three subsets of chromosome-specific probes with each set comprised of 8 different probes. Also described are assays using a set of probes to analyze single cells and cellular organelles to accurately determine the number and type of targeted human chromosomes in various types of cells and cell organelles, such as tumor cells, interphase cells and first polar bodies biopsied from non-inseminated oocytes. Methods of selection or generation of suitable probes and hybridization protocols are described, as are preferred probes for frill set of 24 chromosome-specific probes to target all 24 human chromosomes are described in the Tables. | 04-16-2009 |
| 20090098535 | UPREGULATION OF RACK-1 IN MELANOMA AND ITS USE AS A MARKER - The present invention concerns a method for diagnosing a melanoma in a mammal comprising the detection of the overexpression of RACK-1 protein in a melanocytic cell of said mammal, and the deduction of the presence of a melanoma from the overexpression of RACK-1 protein. The invention is also directed to a method for determining the tumoral status of a melanocytic cell of a mammal, comprising the detection of overexpression of RACK-1 protein in the melanocytic cell, and the deduction of the tumoral state of said cell from the overexpression of RACK-1 protein. | 04-16-2009 |
| 20090098536 | Method for subgroup analysis in subjects having or being suspected of having inflammatory disease, use of anti-p38MAPK antibodies, kits and their use - The present invention relates to a method of determining whether a subject having or suspected of having inflammatory disease may potentially benefit from treatment with a p38 mitogen-activated protein kinase (p38MAPK) inhibitor, which method comprises
| 04-16-2009 |
| 20090098537 | Biomarker for Sensitivity to mTOR Inhibitor Therapy in Kidney Cancer - The invention disclosed herein provides methods for the examination and/or quantification of biochemical pathways that are disregukted in pathologies such as cancer and to reagents and kits adapted for performing such methods. For example a correlation between VHL loss and mTOR inhibitor sensitivity in human kidney cancer cells is disclosed, indicating that VHL loss confers autonomous and angiogenic competitive advantages to such cells. | 04-16-2009 |
| 20090098538 | Prognostic and diagnostic method for disease therapy - The present invention provides novel methods and kits for diagnosing the presence of cancer within a patient, and for determining whether a subject who has cancer is susceptible to different types of treatment regimens. The cancers to be tested include, but are not limited to, prostate, breast, lung, gastric, ovarian, bladder, lymphoma, mesothelioma, medullablastoma, glioma, and AML. Identification of therapy-resistant patients early in their treatment regimen can lead to a change in therapy in order to achieve a more successful outcome. One embodiment of the present invention is directed to a method for diagnosing cancer or predicting cancer-therapy outcome by detecting the expression levels of multiple markers in the same cell at the same time, and scoring their expression as being above a certain threshold, wherein the markers are from a particular pathway related to cancer, with the score being indicative or a cancer diagnosis or a prognosis for cancer-therapy failure. This method can be used to diagnose cancer or predict cancer-therapy outcomes for a variety of cancers. The markers can come from any pathway involved in the regulation of cancer, including specifically the PcG pathway and the “stemness” pathway. The markers can be mRNA, microRNA, DNA, or protein. | 04-16-2009 |
| 20090098539 | Methods and Kits for Detecting an Enzyme Capable of Modifying a Nucleic Acid - An improved method of detecting an enzyme in a sample, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule, thereby conferring the nucleic acid molecule with the ability to be extended to generate a novel detectable nucleic acid molecule, comprises the steps of allowing the sample to be tested for the presence of the enzyme to interact with the nucleic acid molecule; and testing for interaction of the enzyme with the nucleic acid molecule by detecting the novel nucleic acid molecule generated only in the presence of the enzyme. The preferred enzyme is a phosphatase. The methods have a number of applications, for example in enhancing the sensitivity of immunoassays, for detecting pathogen associated phosphatase, for diagnosing certain conditions and for detecting specific contaminants in a sample. | 04-16-2009 |
| 20090098540 | RECIRCULATING MICROFLUIDIC DEVICE AND METHODS OF USE - The present invention relates to a microfluidic test device for detecting or quantifying an analyte in a test sample. The device includes a non-absorbent substrate having at least one microchannel imbedded in the substrate, a non-specific capture device, and one or more stationary mixing structures extending into the at least one microchannel. The present invention also relates to relates to various methods of using the microfluidic test device to detect or quantify an analyte in a test sample. The present invention also relates to a microfluidic device that includes a non-absorbent substrate having at least one microchannel imbedded in the substrate and one or more stationary mixing structures extending into the at least one microchannel. | 04-16-2009 |
| 20090098541 | Devices and processes for analysing individual cells - A device for individually analysing cells of interest, comprising (a) a channel for receiving the contents of a cell of interest, wherein the channel has an input end and an output end, and (b) a cell trapping site in proximity to the input end of the channel, wherein (i) the input end of the channel is adapted such that an intact cell of interest cannot enter the channel; and (ii) the channel contains one or more analytical components for analysing the contents of the cell of interest. In use, a cell is applied to the device, where it is trapped by the cell trapping means. The cell cannot enter the channel intact, but its contents can be released in situ to enter the channel's input end. The contents can then move down the channel, towards the output end, and they encounter the immobilised reagents, thereby permitting analysis of the cell contents. | 04-16-2009 |
| 20090098542 | Gene Methylation in Colon Cancer Diagnosis - The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with colon cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use the detection and diagnosis of colon cancer. | 04-16-2009 |
| 20090098543 | GENE METHYLATION IN LUNG CANCER DIAGNOSIS - The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with lung cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use the detection and diagnosis of lung cancer. | 04-16-2009 |
| 20090098544 | MARKER MOLECULES ASSOCIATED WITH LUNG TUMORS - The present invention relates to nucleic acids and polypeptides associated with lung cancer. The invention is more specifically related to a nucleic acids and the polypeptides transcribed thereof, the expression of which is significantly altered in association with lung cancer. The invention relates to a series of differentially spliced transcripts of the gene disclosed herein, that are associated with tumors of the respiratory tract. Furthermore the present invention provides a method for early diagnosis, prognosis and monitoring of the disease course and for therapy and vaccination of cell proliferative disorders such as e.g. lung tumors. | 04-16-2009 |
| 20090098545 | IN VITRO ASSAY FOR IDENTIFICATION OF ALLERGENIC PROTEINS - The present invention relates to a process for in vitro evaluation, of a potentially allergenic or tissue irritating sub-stance whereby test cells are cultivated in the presence of the substance, and the presence of up regulated genes chosen from G1P2, OASL, IFIT1, TRIM22, IFI44L, MXI, RSAD2, IFIT3, IFITM1, IFIT2, SPR, GNB2, XK, IFITM3, C 33.28 HERV-H protein mRNA, IFITM3, XK, GPR15, MT1G, MT1B; MT1A, ADFP, JL8, MT1E, MT1F, MT1H, SLC30A1, SERPINB2, CD83, TncRNA or expression products from them are measured. The invention also regards use of the expression products from one or more of the genes for in vitro analysis of allergy or tissue irritation. It also relates to a probe comprising at least three nucleic acids, preferably 3-40, especially 5-15 chosen from RNA complementary to the RNA corresponding to any of the genes and the use thereof for in vitro analysis of allergy or tissue irritation. Further it regards a reagent kit comprising one or more probes that recognize products produced during the expression of any the genes. | 04-16-2009 |
| 20090098546 | Methods of Screening for LTRPC7 Modulators - The present invention relates to the identification and isolation of a novel family of ATP regulated calcium transmembrane channel polypeptides designated herein as “LTRPC7” (Long Transient Receptor Potential Channel). Channels comprising these polypeptides close in response to concentrations of cytoplasmic ATP in the millimolar range, are subject to inhibition by high intracellular levels of calcium and/or magnesium, and do not respond to depletion or reduction in intracellular calcium stores. The invention further relates to the methods of utilizing LTRPC7 for binding, and the methods for modulating LTRPC7 activity and for measuring LTRPC2 permeability. The invention further relates to the methods of modulating expression of LTRPC7. | 04-16-2009 |
| 20090098547 | Methods for Identifying DNA Copy Number Changes Using Hidden Markov Model Based Estimations - Methods for estimating genomic copy number and loss of heterozygosity using Hidden Markov Model based estimation are disclosed. | 04-16-2009 |
| 20090098548 | MODULATORS OF ENZYMATIC NUCLEIC ACID ELEMENTS MOBILIZATION - The present invention discloses a nucleic acid cleavage assay for members of the transposase/integrase superfamily. A method of using the assay to screen for modulators of the nucleic acid cleavage activity is also disclosed. The present invention further provides a method for screening for modulators of binding of a transposase/integrase to its corresponding recognition sequence. In addition, the present invention provides a method of identifying a modulator for a particular transposase/integrase such as HIV integrase based on modulators of other members of the transposase/integrase superfamily. Also disclosed are Tn5 transposase inhibitors and HIV integration inhibitors. | 04-16-2009 |
| 20090098549 | SELEX AND PHOTOSELEX - The present disclosure describes improved SELEX methods for generating nucleic acid ligands that are capable of binding to target molecules and improved photoSELEX methods for generating photoreactive nucleic acid ligands that are capable of both binding and covalently crosslinking to target molecules. The disclosure further describes nucleic acid libraries having expanded physical and chemical properties and their use in SELEX and photoSELEX; methods for increasing the crosslinking efficiencies of photoaptamers; methods for producing photoaptamers having selective modifications that enhance functionality and minimize non-specific photoreactions; and methods for generating truncated nucleic acid ligands from nucleic acid ligands of longer length. The disclosure further describes aptamers and photoaptamers obtained by using any of the foregoing. | 04-16-2009 |
| 20090098550 | MRI CONTRAST AGENTS AND HIGH-THROUGHPUT SCREENING BY MRI - The present invention provides an MRI contrast agent, comprising: MRI contrast agent particles, and oligonucleotides, attached to the particles. | 04-16-2009 |
| 20090098551 | METHODS AND PRODUCTS RELATED TO GENOTYPING AND DNA ANALYSIS - The invention encompasses methods and products related to genotyping. The method of genotyping of the invention is based on the use of single nucleotide polymorphisms (SNPs) to perform high throughput genome scans. The high throughput method can be performed by hybridizing SNP allele-specific oligonucleotides and a reduced complexity genome (RCG). The invention also relates to methods of preparing the SNP specific oligonucleotides and RCGs, methods of fingerprinting, determining allele frequency for a SNP, characterizing tumors, generating a genomic classification code for a genome, identifying previously unknown SNPs, and related compositions and kits. | 04-16-2009 |
| 20090098552 | METHODS AND COMPOSITIONS FOR ANALYZING AHASL GENES - The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of | 04-16-2009 |
| 20090098553 | Urine gene expression ratios for detection of cancer - This invention relates to methods for determining the presence of cancer in a subject based on the analysis of the expression levels of an under-expressed tumour marker (TM) and at least one other TM. Specifically, this invention relates to the determination of a cancer, particularly bladder cancer, by performing ratio, regression or classification analysis of the expression levels of at least one under-expressed TM, particularly an under-expressed bladder TM (BTM), and at least one over-expressed TM, particularly an over-expressed BTM. In various aspects, the invention telates to kits and devices for carrying out these methods. | 04-16-2009 |
| 20090098554 | Method and composition for cancer diagnosis and treatment - Methods and compositions for inhibiting the onset of cancer, and cancer diagnosis and treatment are provided. The treatment method comprises inhibiting the level or function of transcriptional positive factor 4 (PC4). Also provided are methods of screening for cancer inhibition agents based on inhibition of PC4 expression or function. | 04-16-2009 |
| 20090098555 | METHODS AND APPLICATIONS FOR STITCHED DNA BARCODES - Methods of identifying the genotypes of a plurality of single cells, wherein each cell includes a plurality of DNA barcodes, each associated with a genetic mutation or marker, are provided. In particular, methods including linking a plurality of DNA barcodes together to create a stitched barcode, amplifying the stitched barcode and sequencing the stitched barcode are provided. Also provided are methods of determining the presence of at least one genetic mutation in a population of cells. | 04-16-2009 |
| 20090098556 | Genetic Test For PSE-Susceptible Turkeys - This invention relates to methods and compounds for the improvement of turkey meat and turkey populations, but not limited to, a genetic screen to select for turkeys that produce a better quality of meat characterized by a higher postmortem pH and better water holding capacity. | 04-16-2009 |
| 20090098557 | IDENTIFICATION OF GENETIC MARKERS ASSOCIATED WITH PARKINSON DISEASE - The present invention provides methods and compositions for screening a subject for Parkinson disease, for increased risk of developing Parkinson disease and/or for an earlier or later age of developing Parkinson disease, comprising detecting the presence of a genetic marker associated with Parkinson disease. | 04-16-2009 |
| 20090098558 | DETECTION, IDENTIFICATION AND DIFFERENTIATION OF PROTEUS SPECIES USING THE SPACER REGION - The present invention relates to new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribionucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of | 04-16-2009 |
| 20090098559 | FORENSIC SWAB AND KIT - The present invention relates to a high sensitivity crime scene swab device for maximal recovery of trace forensic DNA evidence left at a crime scene for DNA PCR analysis. More particularly, DNA recovery is obtained from fingerprints lifted from surfaces at the crime scene. The invention also relates to a high sensitivity method for DNA analysis of trace DNA obtained by generating small tandem repeat (STR) profiles using a polymerase chain reaction protocol. | 04-16-2009 |
| 20090098560 | PURIFICATION METHOD AND KITS - A method for separating nucleic acid from a liquid sample, said method comprising the steps of causing a liquid sample containing or suspected of containing said nucleic acid to flow along a bibulous membrane, for example of a conventional lateral flow device, so that nucleic acid is distributed along the length of the membrane. The nucleic acid may be detected on the membrane. | 04-16-2009 |
| 20090098561 | Higher-order cellular information processing devices - The invention provides various signal processing devices for integrating two or more biological signals (e.g., the presence, absence or concentration of specific ligands, etc.) to generate a status output, or a response that modulates one or more biological activities based on the status of the biological signals. The various described signal processing/integration mechanisms may be combined with one another to provide the device with more flexibility in integrating high-order cellular information. The signal processing devices of the invention have many uses in various biological systems, including gene expression control or ligand-concentration sensing. | 04-16-2009 |
| 20090098562 | Methods for identifying stem cells based on nuclear morphotypes - Methods for identifying stem cells and other cells specific to embryogenesis and carcinogenesis, classifying tissue samples, diagnosing precancerous and cancerous or atherosclerotic lesions, testing the value of anticancer agents, discovering macromolecules specifically expressed in particular cell types, using stem cells in restorative tissue therapy as well as methods for preparing tissue samples so heteromorphic nuclear morphotypes remain intact are disclosed. | 04-16-2009 |
| 20090098563 | Diagnosis of (a risk of) disease and monitoring of therapy - Provided are methods for typing a sample of an individual suffering from, or at risk of suffering from, a disease and a method for monitoring treatment of an individual suffering from a disease comprising determining whether a sample from the individual comprises an expression product of AC133 in an amount that is indicative for the disease or for the treatment thereof. That amount may be quantified and compared with a reference value. In one aspect, the amount is compared with an amount of the expression product present in a sample that was obtained from the individual before treatment. Use of a nucleic acid molecule comprising at least part of a sequence of AC133, or an analogue thereof, for monitoring a treatment of an individual suffering from a disease is also provided, as well as a diagnostic kit comprising such nucleic acid molecule. | 04-16-2009 |
| 20090098564 | Biomarkers for diagnosing schizophrenia and bipolar disorder - The invention relates to the identification and selection of novel biomarkers and the identification and selection of novel biomarker combinations which are differentially expressed in blood and useful in diagnosing schizophrenia and/or bipolar disorder as well as monitoring therapeutic efficacy of treatment for schizophrenia or bipolar disorder. The measurement of expression levels of the products of the biomarkers and combinations of biomarkers of the invention can be used to diagnose schizophrenia and/or bipolar disorder. Measurement of the expression level of products of biomarkers of the invention using polynucleotides and proteins which specifically and/or selectively hybridize to the products of the biomarkers of the invention are also encompassed within the scope of the invention as are compositions and kits containing said polynucleotides and proteins. Further encompassed by the invention is the use of the polynucleotides and proteins to monitor the efficacy of therapeutic regimens. The invention also provides for the identification of methods of using the products of the biomarkers of the invention in the identification of novel therapeutic targets of schizophrenia and/or bipolar disorder and a method of screening the genes of said biomarkers for additional markers of disease. | 04-16-2009 |
| 20090098565 | MeCP2E1 gene - The invention is a novel MECP2E1 splice variant and its corresponding polypeptide. The invention also includes methods of using these nucleic acid sequences and proteins in medical diagnosis and treatment of neuropsychiatric disorders or development disorders. | 04-16-2009 |
| 20090098566 | METHOD OF SYNTHESIZING NUCLEIC ACID - The present invention relates to an oligonucleotide having a novel structure and a method of synthesizing nucleic acid by using the same as a primer. This oligonucleotide is provided at the 5′-side of the primer with a nucleotide sequence substantially the same as a region synthesized with this primer as the origin of synthesis. The present invention realizes synthesis of nucleic acid based on an isothermal reaction with a simple constitution of reagents. Further, the present invention provides a method of synthesizing highly specific nucleic acid on the basis of this method of synthesizing nucleic acid. | 04-16-2009 |
| 20090098567 | COLLECTION DEVICE - A cap which can form an essentially leak-proof seal with an open-ended vessel capable of receiving and holding fluid specimens or other materials for analysis. To minimize potentially contaminating contact between a fluid sample present in the vessel and humans or the environment, the present invention features a cap having a frangible seal which is penetrable by a plastic pipette tip or other fluid transfer device. The cap further includes a filter for limiting dissemination of an aerosol or bubbles once the frangible seal has been pierced. The filter is positioned between the frangible seal and a retaining structure. The retaining structure is positioned on the cap above the filter and may be used to contain the filter within the cap. The material of the retaining structure may be penetrable by a fluid transfer device. | 04-16-2009 |
| 20090104598 | Dopamine D2 receptor gene variants - The present invention provides a method of predicting antipsychotic response to drug therapy comprising testing a sample obtained from a subject for the presence of a polymorphism in the dopamine D2 receptor gene DRD2, wherein the presence of the rs1079598T allele, the rs1125394A allele or both is predictive of the subject being susceptible to drug therapy. | 04-23-2009 |
| 20090104599 | GENETIC VARIANT OF THE ANNEXIN A5 GENE - The present invention relates to a nucleic acid molecule comprising an annexin A5 (ANXA5) gene regulation element which comprises at least one point mutation, whereby said at least one point mutation (substitution) is selected from the group consisting of (i) a point mutation G to A at a position which corresponds to nucleotide 186 of SEQ ID NO: 2; (ii) a point mutation A to C at a position which corresponds to nucleotide 203 of SEQ ID NO: 2; (iii) a point mutation T to C at a position which corresponds to nucleotide 229 of SEQ ID NO: 2; and (iv) a point mutation G to A at a position which corresponds to nucleotide 276 of SEQ ID NO: 2. Furthermore, the present invention provides for a vector comprising the nucleic acid molecule the invention and a host transformed with the vector. The invention also relates to specific uses, in particular diagnostic uses of the nucleic acid molecules described herein. Moreover, the invention relates to a method for haplotyping an ANXA5 gene regulation element in an individual comprising the steps of: (a) isolating a nucleic acid from a sample that has been removed from the individual; (b) determining the presence of the nucleotides present at positions 186, 203, 229 and 276 of the individual's copy of the ANXA5 gene regulation element, wherein the position numbers are determined by comparison to SEQ ID NO: 2; (c) assigning the individuals a particular haplotype by comparison of the nucleotides present at said positions to the nucleotides recited in the haplotypes as defined herein. | 04-23-2009 |
| 20090104600 | Mutation within the connexin 26 gene responsible for prelingual non-syndromic deafness and method of detection - A purified polynucleotide having a chain of nucleotides corresponding to a mutated sequence, which in a wild form encodes a polypeptide implicated in hereditary sensory defect, wherein said mutated purified polynucleotide presents a mutation responsible for prelingual non-syndromic deafness selected from the group consisting of a specific deletion of at least one nucleotide. | 04-23-2009 |
| 20090104601 | GENETIC DIAGNOSIS USING MULTIPLE SEQUENCE VARIANT ANALYSIS - The present invention is in the field of nucleic acid-based genetic analysis. More particularly, it discloses novel insights into the overall structure of genetic variation in all living species. The structure can be revealed with the use of any data set of genetic variants from a particular locus. The invention is useful to define the subset of variations that are most suited as genetic markers to search for correlations with certain phenotypic traits. Additionally, the insights are useful for the development of algorithms and computer programs that convert genotype data into the constituent haplotypes that are laborious and costly to derive in an experimental way. The invention is useful in areas such as (i) genome-wide association studies, (ii) clinical in vitro diagnosis, (iii) plant and animal breeding, (iv) the identification of micro-organisms. | 04-23-2009 |
| 20090104602 | Diagnosis of Tuberculosis - The invention provides a method of diagnosing tuberculosis (TB) in a test subject, said method comprising: (i) providing expression data of two or more markers in a subject, wherein at least two of said markers are selected from transthyretin, neopterin, C-reactive protein (CRP), serum amyloid A (SAA), serum albumin, apoliopoprotein-A1 (Apo-A1), apolipoprotein-A2 (Apo-A2), hemoglobin beta, haptoglobin protein, DEP domain protein, leucine-rich alpha-2-glycoprotein (A2GL) and hypothetical protein DFKZp667I032; and (ii) comparing said expression data to expression data of said marker from a group of control subjects, wherein said control subjects comprise patients suffering from inflammatory conditions other than TB, thereby determining whether or not said test subject has TB. | 04-23-2009 |
| 20090104603 | Tissue Carbohydrate Compositions and Analysis Thereof - The present invention reveals novel methods for producing novel carbohydrate compositions, glycomes, from animal tissues. The tissue substrate materials can be total tissue samples and fractionated tissue parts, or artificial models of tissues such as cultivated cell lines. The invention is further directed to the compositions and compositions produced by the methods according to the invention. The invention further represent methods for analysis of the glycomes, especially mass spectrometric methods. | 04-23-2009 |
| 20090104605 | DIAGNOSIS OF SEPSIS - Methods and apparatus for predicting the development of sepsis in a subject at risk for developing sepsis are provided. Features in a biomarker profile of the subject are evaluated. The subject is likely to develop sepsis if these features satisfy a particular value set. Methods and apparatus for predicting the development of a stage of sepsis in a subject at risk for developing a stage of sepsis are provided. A plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to have the stage of sepsis if these feature values satisfy a particular value set. Methods and apparatus for diagnosing sepsis in a subject are provided. A plurality of features in a biomarker profile of the subject is evaluated. The subject is likely to develop sepsis when the plurality of features satisfies a particular value set. | 04-23-2009 |
| 20090104606 | LATE GESTATION LUNG GENES, FRAGMENTS AND USES THEREOF - The present invention provides a family of genes related to late gestation lung genes and fragments thereof. Embodiments of the present invention provide compositions and methods for the therapeutic treatment of disorders in the lung or other tissues. More particularly the invention provides methods for the treatment of abnormalities in alveolarization, and abnormalities in branching morphogenesis. In other embodiments of the invention the use of the LGL1 gene or related products or fragments thereof in research and diagnostics is provided. | 04-23-2009 |
| 20090104607 | EUBACTERIAL tmRNA SEQUENCES AND USES THEREOF - The present invention is directed to eubacterial tmDNA sequences and the corresponding tmRNA sequences. The present invention is further directed to alignments of eubacterial tmDNA sequences and the use of the sequences and sequence alignments for the development of antibacterial drugs. The present invention is also directed to the use of the sequences for the development of diagnostic assays. | 04-23-2009 |
| 20090104608 | EUBACTERIAL tmRNA SEQUENCES AND USES THEREOF - The present invention is directed to eubacterial tmDNA sequences and the corresponding tmRNA sequences. The present invention is further directed to alignments of eubacterial tmDNA sequences and the use of the sequences and sequence alignments for the development of antibacterial drugs. The present invention is also directed to the use of the sequences for the development of diagnostic assays. | 04-23-2009 |
| 20090104609 | METHOD FOR DISCRIMINATION OF METAPLASIAS FROM NEOPLASTIC OR PRENEOPLASTIC LESIONS - The present invention relates to a method for discrimination of p16 | 04-23-2009 |
| 20090104611 | METHODS OF WHOLE GENOME ANALYSIS - Methods are provided for ascertaining the sequence of a genomic DNA sample by nicking the DNA sample with a restriction nicking enzyme, followed by nick translation with labeled nucleotides, such that the labeled nucleotides can be quantified and compared to a known, reference genome. | 04-23-2009 |
| 20090104612 | DETECTION OF BLOOD GROUP GENES - Disclosed herein are nucleic acid molecules which permit the accurate and direct determination of blood groups based on the presence of certain genes. A method of determining blood groups is also provided. | 04-23-2009 |
| 20090104613 | METHODS AND COMPOSITIONS RELATING TO MULTIPLEXED GENOMIC GAIN AND LOSS ASSAYS - Compositions and methods are provided for detecting genomic DNA gain and loss. Embodiments of inventive assays include using a substrate-attached composite nucleic acid probe which specifically hybridizes to two or more genomic loci in a genomic region of a reference genome. The genomic region is characterized by a first terminus and a second terminus and has an intermediate region disposed between the first terminus and second terminus of at least 400 kilobases. The composite nucleic acid probe includes nucleic acid sequences which specifically hybridize to substantially an entire first genomic locus including the first terminus and to substantially an entire second genomic locus including the second terminus. Methods and compositions are provided which include assessment of two or more genomic DNA references. | 04-23-2009 |
| 20090104614 | QUANTITATIVE MOLECULAR PROBES - In accordance with this invention, a molecular probe for detection of a nucleic acid target containing a preselected target sequence is constructed and has at least two sources of a signal: a conventional reporter source and a reference source in a form of a luminescent material, e.g., a fluorophore, quantum dot, fluorescent nanoparticle, or other fluorescent reference dye/nanoparticle/microparticle conjugated to the molecular probe. | 04-23-2009 |
| 20090104615 | PHENOTYPE PREDICTION - Detection of epigenetic alteration, especially methylation, of a gene or a combination of genes, preferably in a perinatal tissue sample such as umbilical cord, for predicting diverse phenotypic characteristics such as propensity for obesity, altered body composition, impaired cognition, low bone mineral content, neuro-behavioural problems and altered cardiovascular structure and function occurring in an individual. | 04-23-2009 |
| 20090104616 | PRIMER SET FOR AMPLIFYING SULT1A1 GENE, REAGENT FOR AMPLIFYING SULT1A1 GENE CONTAINING THE SAME, AND THE USES THEREOF - A primer set for amplifying a region including sites to be detected of SULT1A1*2 and SULT1A1*3 in the SULT1A1 gene by a gene amplification method is provided, wherein the primer set can amplify the region specifically. A pair of primer set is used including a forward primer consisting of the base sequence of SEQ ID NO: 7 as well as a reverse primer consisting of the base sequence of SEQ ID NO: 18. The use of this primer set makes it possible to specifically and efficiently amplify, a region including both sites where two types of polymorphisms (SULT1A1*2 and SULT1A1*3) of the SULT1A1 gene are generated. | 04-23-2009 |
| 20090104617 | Diagnostic and Prognostic Tests - The invention provides methods for diagnosing biological states or conditions based on ratios of gene expression data from tissue samples, such as cancer tissue samples. The invention also provides sets of genes that are expressed differentially in malignant pleural mesothelioma. These sets of genes can be used to discriminate between normal and malignant tissues, and between classes of malignant tissues. Accordingly, diagnostic assays for classification of tumors, prediction of tumor outcome, selecting and monitoring treatment regimens and monitoring tumor progression/regression also are provided. | 04-23-2009 |
| 20090104618 | Psoriasin Expression By Breast Epithelial Cells - The invention features methods of diagnosing high grade ductal carcinoma in situ (DCIS) These methods involve measuring: (1) the level of HID-5 in a body fluid (e.g., blood or urine) of a subject suspected of having, or at risk of having, high grade DCIS; or (2) the level of HID-5 gene expression in breast tissue from a subject suspected of having, or at risk of having, high grade DCIS. The invention also embodies a method of inhibiting expression of HID-5 protein in DCIS cells and methods of treating a subject suspected of having, or at risk of having, high grade DCIS. | 04-23-2009 |
| 20090104619 | Human G-Protein Coupled Receptors - Two human G-Protein coupled receptor polypeptides and DNA (RNA) encoding each of such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptides for identifying antagonists and agonists to such polypeptides. Also disclosed are diagnostic methods for detecting a mutation in the nucleic acid sequence of each of the G-protein coupled receptors. | 04-23-2009 |
| 20090104620 | Simplified Method of Determining Predisposition to Scoliosis - The present invention relates to novel genetic markers associated with scoliosis, risk of developing scoliosis and risk of scoliosis curve progression, and simplified methods and materials for determining whether a human subject has scoliosis, is at risk of developing scoliosis or is at risk of scoliosis curve progression. | 04-23-2009 |
| 20090104621 | METHOD FOR DETECTING A TARGET NUCLEIC ACID SEQUENCE - A method of detecting a target nucleic acid sequence comprising providing a stem-and-loop structured nucleic acid for measurement wherein the nucleic acid comprises complementary sequence portions located at both terminals and a target sequence portion therebetween as well as a double-stranded portion formed by hybridization of the complementary sequence portions located at both terminals and a remaining looped single-stranded portion, providing a probe nucleic acid having a sequence complementary to the target sequence portion wherein one end of the probe nucleic acid being immobilized to a solid substrate surface, reacting the nucleic acid for measurement with the probe nucleic acid to specifically hybridize the target sequence portion of the nucleic acid for measurement to the probe nucleic acid, and detecting presence or absence of the nucleic acid for measurement hybridized to the probe nucleic acid. | 04-23-2009 |
| 20090111093 | Methods and compositions for pre-symptomatic or post-symptomatic diagnosis of alzheimer's disease and other neurodegenerative disorders - Methods, compositions and apparatus (e.g., test kits, test systems, reagents, related computer software, calculators, etc.) for pre-symptomatic or post-symptomatic diagnosis of Alzheimer's Disease or other disorders associated with the formation of β-amyloid deposits (e.g., plaques) and/or β-amyloid fibrils. Also, methods, compositions and apparatus assessing the efficacy of treatments for such disorders. Sample cells, tissue or body fluid are obtained from a human or animal subject and analyzed to determine whether or to what extent certain mitochondrial DNA control region (mtDNA CR). Significantly elevated numbers of these mtDNA CR mutations may indicate that the subject suffers from, or is at increased risk for development of, Alzheimer's Disease or other disorders associated with the formation of β-amyloid deposits (e.g., plaques) and/or β-amyloid fibrils. A significant decrease in the numbers of these mtDNA CR mutations during treatment for the disorder may indicate that the treatment is effective. | 04-30-2009 |
| 20090111094 | Methods for preparing hybrid substrates comprising DNA and antibodies and uses thereof - The invention provides substrates that have antibodies and aptamers bound thereto. The invention also provides methods of detecting target analytes in a sample comprising detecting binding of a target analyte to capture probes on a substrate, wherein some of the capture probes comprise antibodies and other capture probes comprise aptamers, and all of the capture probes are bound to the substrate. In addition, the invention provides substrates that have capture probes and capture oligonucleotides bound thereto, wherein the capture oligonucleotides can hybridize to DNA barcodes. The invention also provides methods of detecting target analytes in a sample comprising contacting the sample with a substrate that has capture probes and capture oligonucleotides bound thereto. | 04-30-2009 |
| 20090111095 | NOVEL DIAGNOSTIC KIT FOR MALIGNANT MELANOMA - The object of the present invention is to find out another tumor marker which is useful for early diagnosis of melanoma, and provide a diagnostic kit and diagnostic method for malignant melanoma using such marker. The present invention provides a diagnostic kit for malignant melanoma, which comprises an antibody against SPARC and an antibody against GPC3. | 04-30-2009 |
| 20090111096 | Method of exhaustive analysis of transcriptionally-active domain (non-methylated domain) on genome - The purpose of the present invention is to provide a method to simultaneously detect a number of the non-methylated regions in the genome of two or more types of cells, and to exhaustively compare and analyze them. | 04-30-2009 |
| 20090111097 | Comparative Analysis of Extracellular RNA Species - The invention provides methods for detecting tumor-associated RNA in plasma, serum, and other bodily fluids. In particular, the invention provides methods for detecting translocated gene RNA, including fusion gene RNA, in plasma or serum or other bodily fluids. | 04-30-2009 |
| 20090111098 | REPORTER GENE ASSAY - The invention provides a more highly accurate assay for measuring the transcriptional activity of a test substance. Disclosed is a reporter gene assay, comprising the steps of contacting a cell having a vector wherein a reporter gene containing a gene encoding an epitope tag is ligated downstream to a recognition sequence of a transcription factor and a nucleotide sequence necessary for transcriptional initiation, with a test substance and detection antibodies; detecting a phenomenon caused by the two kinds of detection antibodies coming close to each other; and correlating the detected phenomenon with the effect of the test substance on transcriptional regulatory mechanism. | 04-30-2009 |
| 20090111099 | Promoter Detection and Analysis - The present disclosure discloses an array-based method for promoter detection and analysis. Promoter sequence candidates are analyzed simultaneously in one reaction vial utilizing a vector comprising a TAG sequence wherein transcriptional products are tagged as they are synthesized, in such a way that one specific transcript is labeled with only one type of tag, and one tag labels only one type of transcript. The transcriptional output is analyzed on conventional arrays. | 04-30-2009 |
| 20090111100 | MINOR GROOVE BINDER - ENERGY TRANSFER OLIGONUCLEOTIDES AND METHODS FOR THEIR USE - The incorporation of a minor groove binder spaced close to one member of a matched FRET set in a minor groove binder-oligonucleotide conjugate significantly reduces background fluorescence of a FRET probe or pair of probes and, consequently, increases the S/B ratios. Fluorescent-labeled probes are useful in carrying out hybridization, multiplex nucleic acid detection, and other procedures. | 04-30-2009 |
| 20090111101 | Automated Cancer Diagnostic Methods Using FISH - In various embodiments methods for automated screening for gene amplification in biological tissue samples using an automated fluorescence microscope to analyze fluorescence in situ hybridized samples are provided. Various additional embodiments provide methods of high throughput screening for gene amplification. | 04-30-2009 |
| 20090111102 | METHODS FOR DETECTING INFLAMMATORY BOWEL DISEASE - The present invention provides for a method of detecting the presence of inflammatory bowel disease in gastrointestinal tissues or cells of a mammal by detecting increased expression of LY6 genes in the tissues or cells of the mammal relative to a control. | 04-30-2009 |
| 20090111103 | METHOD FOR MEASURING THE NUMBER OF ORAL LACTOBACILLUS, AND A PCR PRIMERS-PROBE SET USED FOR THE SAME - There is provided a real-time PCR assay capable of measuring the number of | 04-30-2009 |
| 20090111104 | Method for Monitoring Hydrolytic Activity - The present invention relates to methods of measuring the activity of a hydrolytic agent comprising contacting a biomolecule with a hydrolytic agent in the presence of a fluorescent dye under conditions that allow digestion of the biomolecule by the hydrolytic agent. The fluorescence of the dye is monitored over time and a change in fluorescence signifies digestion of the biomolecule by the hydrolytic agent. The biomolecule is preferably a protein, peptide or proteome but can also be a carbohydrate, oligonucleotide or lipid. Further methods relate to determining an end point for digestion of a biomolecule by a hydrolytic agent, and methods of monitoring digestion of a biomolecule by a hydrolytic agent. The monitoring can be performed on the reaction mixture in real time or via sampling. The invention also relates to kits for carrying out the method. | 04-30-2009 |
| 20090111105 | ANDROGEN-DEPENDENT 1-F-AROMATASE REPORTER GENE - The present invention relates to an androgen-dependent 1-f-aromatase reporter gene and a method for the production of the 1-f-aromatase-reporter gene and use thereof in a method for identifying ligands of the androgen receptor. | 04-30-2009 |
| 20090111106 | Vector System - The present invention provides a vector system comprising a mutated post-transcriptional regulatory element. In particular, the present invention relates to a mutated WPRE sequence that can efficiently express nucleotides of interest in a retroviral vector system. The present invention also relates to methods of delivering and expressing nucleotides of interest to a target cell. | 04-30-2009 |
| 20090111107 | TUMOR SUPPRESSOR GENE P33ING2 - The invention provides isolated nucleic acid and amino acid sequences of novel human tumor suppressors, antibodies to such tumor suppressors, methods of detecting such nucleic acids and proteins, methods of screening for modulators of tumor suppressors, and methods of diagnosing and treating tumors with such nucleic acids and proteins. | 04-30-2009 |
| 20090111108 | Method of Detecting Genetic Mutations - The present invention relates, in general, to drug resistance, and, in particular, to a method of detecting drug resistance populations, including minor drug resistance viral populations. | 04-30-2009 |
| 20090111109 | Genes relating to gastric cancer metastasis - The disclosure provides polynucleotides and polypeptides associated with gastric cancer cells, particularly those having a tendency to metastasize. Also provided are methods and kits for detecting, diagnosing, and/or monitoring metastatic gastric cancer cells. | 04-30-2009 |
| 20090111110 | Method of diagnosing adolescent idiopathic scoliosis and related syndromes causing spinal deformities and method for screening for a compound useful in the treatment of any of these diseases - A method for diagnosing an increased risk for a disease characterized by a dysfunctional melatonin-signaling pathway in an animal comprising detecting the presence or absence of at least one impairment in melatonin-signaling pathway in at least one of the animal's cells, wherein the presence of at least one impairment in melatonin-signaling pathway indicates that the animal possesses an increased risk of developing said disease. and a method of screening for a compound useful in the treatment of a disease characterized by a dysfunctional melatonin-signaling pathway, said method comprising the steps of contacting a candidate compound with at least one cell expressing at least one melatonin-signaling pathway impairment, wherein the candidate compound is selected if said melatonin-signaling pathway impairment is reduced in the presence of the candidate compound as compared to that in the absence thereof. | 04-30-2009 |
| 20090111111 | Detection of Group B Streptococcus - The invention provides methods to detect group B | 04-30-2009 |
| 20090111112 | Nucleic Acid Marker Ladder for Estimating Mass - The invention relates to a nucleic acid marker ladder which is a restriction endonuclease digest, wherein a nucleic acid restriction endonuclease digest is a collection of nucleic acid fragments resulting from complete digestion of one or more nucleic acids by one or more restriction endonucleases; the restriction endonuclease digest contains at least 3 fragments; and the size of the fragments in base pairs is a multiple of an integer, wherein the integer is 10 or more. | 04-30-2009 |
| 20090111113 | SOLID SUPPORT HAVING ELECTROSTATIC LAYER AND USE THEREOF - It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules. | 04-30-2009 |
| 20090111114 | RNA EXTRACTION METHOD, RNA EXTRACTION REAGENT, AND METHOD FOR ANALYZING BIOLOGICAL MATERIALS - A method to extract RNA with high purity from biological materials containing RNA in a safe, rapid, and simple procedure and a method to analyze it are provided. The procedure includes the steps of mixing a biological material containing RNA with a predetermined concentration of a chaotropic agent and a predetermined concentration of an organic solvent, allowing the mixed solution to contact a nucleic acid-binding solid phase, washing the nucleic-acid binding solid-phase to which RNA is bound, and eluting RNA from the nucleic-acid binding solid-phase having the bound RNA. Furthermore, the obtained RNA is analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) or the like. | 04-30-2009 |
| 20090111115 | SEQUENCE ANALYSIS USING DECORATED NUCLEIC ACIDS - The present invention provides a sequence interrogation chemistry that combines the accuracy and haplotype integrity of long-read sequencing with improved methods of preparing genomic nucleic acids and analyzing sequence information generated from those nucleic acids. The present invention encompasses compositions comprising decorated nucleic acids stretched on substrates. The present invention further encompasses methods of making stretched decorated nucleic acids and methods of using decorated nucleic acids to obtain sequence information. | 04-30-2009 |
| 20090111116 | Method for monitoring the efficacy of a mycobacterium avium subspecies paratuberculosis therapy - The present invention relates to | 04-30-2009 |
| 20090111117 | DEVICE AND METHOD FOR DETECTING COMPLEX FORMATION - The present invention provides devices and methods for measuring electrically detectable bulk properties of liquid samples. Representative electrically detectable bulk properties measurable by the devices and methods of the invention include resistivity (conductivity) and dielectric constant (permittivity). The electrically detectable bulk properties are determined by comparing the experimental electrical output of the devices with mathematically simulated models of the experimental devices. | 04-30-2009 |
| 20090111118 | METHOD FOR A RAPID ANTIBODY-BASED ANALYSIS OF PLATELET POPULATIONS - A method for identifying a platelet population, preferably a population of immature, reticulated platelets, in a biological sample involves incubating the biological sample for less than minutes with at least one labeled, ligand (e.g., monoclonal antibody) that binds to an epitope or antigen on platelets and with a nucleic acid dye. In one embodiment, the dye is Acridine Orange and the label on the ligand is PE-Cy7. The sample is then analyzed and one or more platelet populations is rapidly identified or quantified by passing the incubated sample through a sensing region of a flow cytometer. In one embodiment, this method occurs without a washing or physical cell separation step. The incubated sample is irradiated with a laser light source, and fluorescence of the labeled ligand and the nucleic acid dye are measured along with at least one additional parameter, e.g., light scatter, direct current, axial light loss, opacity, radio frequency, and fluorescence. These parameters are used to identify qualitatively or quantitatively the platelet populations in the sample. This rapid analytic method is particularly valuable in clinical situations where either low platelet counts or interfering conditions lead to inaccuracies of the platelet measurement. This method is suitable for performance in an automated hematology analyzer. | 04-30-2009 |
| 20090111119 | Rapid in vivo identification of biologically active nucleases - Disclosed herein are methods and compositions for rapidly identifying and ranking nucleases for specific cleavage of a target sequence. | 04-30-2009 |
| 20090111120 | METHYLATION OF GENES AS A PREDICTOR OF POLYP FORMATION AND RECURRENCE - The present invention provides methods for identifying or assessing probabilities for developing an abnormal condition in subject and for the recurrence of the abnormal condition in the subject after receiving treatment. The method comprises determining the methylation status of at least one gene in the subject and comparing this methylation status to normal methylation status. Differences between the methylation status of the one or more genes is indicative of the subject developing an abnormal condition or for the recurrence of the abnormal conditions after receiving treatment. | 04-30-2009 |
| 20090117538 | Methods for Obtaining Gene Tags - The present invention provides methods for providing as tags the nucleotide sequences at the 5′ end of mRNA. The method of the present invention comprises the step of synthesizing cDNA using, as a template, mRNA whose CAP structure is linked with a IIs linker having a type IIs endonuclease recognition sequence. Tags including the nucleotide sequence from the 5′ end of mRNA are generated by reacting the type IIs endonuclease to cDNA. Tags can be generated from all mRNA, independently of their nucleotide sequences. Methods for identifying transcriptional start sites and primers for full-length cDNA synthesis are provided based on the nucleotide sequence information of tags of the present invention. | 05-07-2009 |
| 20090117539 | DNA sequences for gene suppression - The present invention provides methods of improving DNA sequences for gene suppression mediated by double-stranded RNA, and constructs and transgenic organisms containing such improved DNA sequences. | 05-07-2009 |
| 20090117540 | Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase - The invention relates to compositions and methods for generating a signal indicative of the presence of a target nucleic acid in a sample, where the compositions and methods include an RNA polymerase, a FEN nuclease, and a probe. | 05-07-2009 |
| 20090117541 | METHODS OF DETECTING TPMT MUTATIONS - Methods and compositions are described for use in the rapid and simultaneous screening of one or more samples for one or more mutations in the TPMT gene. The methods and compositions of the present invention can be used to rapidly determine if a mutation of the TPMT gene is present in the genome of a subject. Identifying which mutations are present in an individual allows the clinician to design an appropriate therapy using drugs metabolized by TPMT for that individual. | 05-07-2009 |
| 20090117542 | UNIQUE SHORT TANDEM REPEATS AND METHODS OF THEIR USE - Methods for DNA fingerprinting identification of human DNA samples, comprising: a) exposing a DNA sample of an individual to at least one primer specific for a Y chromosome polymorphism at a predetermined loci, said loci being chosen from OSU9, OSU14, OSU22, OSU35, OSU51, OSU57, OSU67, OSU70, OSU73, OSU77, with the proviso that if OSU70 is selected then at least one other OSU locus is also selected; b) amplifying DNA of the DNA sample using the at least one primer specific for a Y chromosome polymorphism; and c) identifying the size of an amplified product. Primers for the methods are also provided. | 05-07-2009 |
| 20090117543 | METHODS AND COMPOSITIONS FOR INDUCING SIRTUINS - Provided herein are compositions comprising agents that increase the level of expression of a sirtuin gene. Also provided are methods for purifying and isolating a factor from serum that stimulates the expression of a sirtuin gene. Diagnostic methods for determining whether a subject is calorically restricted or resistant to stress are also provided. | 05-07-2009 |
| 20090117544 | METHOD OF DESIGNING PRIMERS FOR USE IN METHOD OF DETECTING TARGET NUCLEIC ACID AND ASSAY KIT - A method of designing primers for use in a method of detecting an amplification product by hybridizing it with a probe, the amplification product is amplified from a target nucleic acid with the primers, including placing F3, F2 and F1 regions in this order from a 5′ terminal side and Bc, B2 | 05-07-2009 |
| 20090117545 | Glycine riboswitches, methods for their use, and compositions for use with glycine riboswitches Cross-Reference to Related Applications - It has been discovered that certain natural mRNAs serve as metabolite-sensitive genetic switches wherein the RNA directly binds a small organic molecule. This binding process changes the conformation of the mRNA, which causes a change in gene expression by a variety of different mechanisms. Modified versions of these natural “riboswitches” (created by using various nucleic acid engineering strategies) can be employed as designer genetic switches that are controlled by specific effector compounds. Such effector compounds that activate a riboswitch are referred to herein as trigger molecules. The natural switches are targets for antibiotics and other small molecule therapies. In addition, the architecture of riboswitches allows actual pieces of the natural switches to be used to construct new non-immunogenic genetic control elements, for example the aptamer (molecular recognition) domain can be swapped with other non-natural aptamers (or otherwise modified) such that the new recognition domain causes genetic modulation with user-defined effector compounds. The changed switches become part of a therapy regimen-turning on, or off, or regulating protein synthesis. Newly constructed genetic regulation networks can be applied in such areas as living biosensors, metabolic engineering of organisms, and in advanced forms of gene therapy treatments. | 05-07-2009 |
| 20090117546 | Disease detection by digital protein truncation assays - Genetic diseases can be diagnosed by detection of mutations in causative genes. Protein truncation assays can be used to detect gene products of truncation-type mutations. However, the sensitivity of the assays is often insufficient to detect mutations present in a sample of DNA at a low frequency. Sensitivity can be increased by dividing samples so that the signal generated by a mutant allele comprises a larger fraction of the total alleles than prior to dividing. Thus a previously undetectable signal generated by the mutant allele can become detectable in the assay. Such increased sensitivity permits detection at early stages and in samples having high levels of other alleles. | 05-07-2009 |
| 20090117547 | NOVEL HAPLOTYPE OF HUMAN T2R RECEPTOR hT2R50 AND ITS USE IN ASSAYS FOR IDENTIFYING HUMAN BITTER TASTE MODULATORS - The present invention relates to the discovery of a novel haplotype of the human taste receptor hT2R50 in the T2R taste receptor family that responds to particular bitter ligands, i.e., 2-acetylpyrazine and ethylpyrazine. The present invention also relates to the use of this novel haplotype in assays for identifying ligands that modulate the activation of the hT2R50 taste receptor. These compounds potentially may be used as additives in foods, beverages and medicinals for modifying (blocking) hT2R50-associated bitter taste. | 05-07-2009 |
| 20090117548 | ANALYSIS OF HIV-1 CORECEPTOR USE IN THE CLINICAL CARE OF HIV-1 INFECTED PATIENTS - A change in viral tropism occurs in many HIV positive individuals over time and can be indicated by a change in coreceptor usage from CCR5 to CXCR4. The change in coreceptor usage to CXCR4 has been shown to correlate with increased disease progression. In patients undergoing HAART, the predominant populations of virus can be shifted back to CCR5-mediated entry after the CXCR4-specific strains have emerged. The present invention relates to a diagnostic method to monitor coreceptor use in the treatment of human immunodeficiency virus (HIV) infection. The present invention further relates to a diagnostic method applied to HIV-positive individuals undergoing HAART to monitor the suppression of CXCR4 specific strains. The diagnostic methods can be used to assist in selecting antiretroviral therapy and to improve predictions of disease prognosis over time. | 05-07-2009 |
| 20090117549 | Aptamer-based methods for identifying cellular biomarkers - In this invention, a biomarker discovery method has been developed using specific biotin-labeled oligonucleotide ligands and magnetic streptavidin beads. In one embodiment, the oligonucleotide ligands are firstly generated by whole-cell based SELEX technique. Such ligands can recognize target cells with high affinity and specificity and can distinguish cells that are closely related to target cells even in patient samples. The targets of these oligonucleotide ligands are significant biomarkers for certain cells. These important biomarkers can be captured by forming complexes with biotin-labeled oligonucleotide ligands and collecting the complexes using magnetic streptavidin beads, whereupon the captured biomarkers are analyzed to identify the biomarkers. Analysis of biomarkers include HPLC-Mass Spectroscopy analysis, polyacrylamide gel electrophoresis, flow cytometry, and the like. The identified biomarkers can be used for pathological diagnosis and therapeutic applications. Using the disclosed methods, highly specific biomarkers of any kinds of cells, in particular cancer cells, can easily be identified without prior knowledge of the existence of such biomarkers. | 05-07-2009 |
| 20090117550 | Assessing brain aneurysms - This document provides methods and materials related to assessing brain conditions within mammals. For example, methods and materials that can be used to determine whether or not a mammal (e.g., a human) with a brain aneurysm is likely to experience brain aneurysm rupture are provided. | 05-07-2009 |
| 20090117551 | METHOD FOR SIMULTANEOUS ANALYSIS OF MULTIPLE BIOLOGICAL REACTIONS OR CHANGES IN IN VIVO CONDITIONS - It is an object of the preset invention to provide an activity measurement molecule necessary for measuring biological reactions or changes in in vivo conditions, and a method for measuring activity using the above activity measurement molecule. It is intended to provide an activity measurement molecule used for simultaneously analyzing multiple biological reactions and/or changes in in vivo conditions, which is characterized in that one or more fluorescent molecule-labeled and/or -unlabeled biomolecules used as targets of the biological reactions or changes in in vivo conditions bind onto a quantum dot, and a method for simultaneously analyzing multiple biological reactions and/or changes in in vivo conditions using the above activity measurement molecule. | 05-07-2009 |
| 20090117552 | Method for Detection and Quantification of Target Nucleic Acids in a Sample - The present invention relates to methods for multiplex detection and quantification of target nucleic acid sequences in a sample comprising the steps of: (i) providing a solid support having immobilized thereon an array of detector oligonucleotides, wherein said array of detector oligonucleotides is designed by random selection of non-eukaryotic genomic sequences followed by random selection of oligonucleotide sequences and subsequent conversion of these oligonucleotide sequences such that these are composed of only three types of nucleotides; (ii) providing a sample having added thereto a fixed amount of control nucleic acid of known sequence; (iii) contacting said sample with at least two probes that hybridise to adjacent sites of a target sequence under conditions favouring hybridisation between the sample nucleic acids and the said at least two probes, wherein, a) a first probe is composed of a 5′ end sequence part for hybridisation to a PCR primer and a 3′ end sequence part for hybridisation to the target nucleic acid; and b) a second probe is composed of a 5′ end sequence part for hybridisation to the target nucleic acid, and a 3′ end sequence part for hybridisation with a PCR primer, and c) an intermediate sequence is present in between said 5′ and 3′ end sequence parts of said first or second probe; and d) said second probe is characterized by having 5′ phosphate group allowing ligation with a 3′ hydroxyl group at the said first probe forming a ligation-mediated probe; (iv) ligation of the said hybridised first and second probes to form ligation-mediated probes; (v) contacting a set of detectable labelled PCR primers with the ligation-mediated probes allowing amplification thereof; (vi) detection and quantification of sample nucleic acids via hybridisation of the said intermediate parts within the amplified ligation-mediated probes onto the array of detector oligonucleotides provided in The present invention also relates to the use of said methods as well as microarrays and kits for performing said methods. | 05-07-2009 |
| 20090117553 | NUCLEIC ACID MELTING ANALYSIS WITH SATURATION DYES - Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided. | 05-07-2009 |
| 20090117554 | POLYNUCLEOTIDE AND PROTEIN INVOLVED IN SYNAPTOGENESIS, VARIANTS THEREOF, AND THEIR THERAPEUTIC AND DIAGNOSTIC USES - The invention relates to a method and a kit for diagnosing autism linked to a mutation in a protein belonging to the family of human neuroligins. | 05-07-2009 |
| 20090117555 | Microfluidic Flow Lysometer Device, System and Method - The invention provides a device, system and method that enables a microfluidic flow lysometer cell analyzer. Using a population of suspended living cells. cell surface molecule detection reagents, and cell cytoplasm (or nuclear) molecule detection reagents, this microfluidic cell analyzer can rapidly analyze a population of cells by running them on a one-at-a-time basis through small capillary channels. The cell's morphology or surface markers are analyzed, then the cells are lysed, and the molecules present in the cell's cytoplasm or nuclear material are analyzed. Cell morphology is then analyzed as the cell surface molecules are correlated with the molecules present in the same cell's cytoplasm or nucleic acids, and this correlated cell population data is then presented to a user for interpretation. The invention also addresses issues such as device fouling, correction for experimental artifacts (incomplete cell lyis, variable cell debris volume effects), and inadequate data collection that hampered earlier efforts in this area. | 05-07-2009 |
| 20090117556 | Association of Single Nucleotide Polymorphisms in the CBFA2T1 and DECR1 Genes with Performance and Carcass Merit of Beef Cattle - Aspects of the present invention also provide novel compositions and methods based on novel CBFA2T1 and/or DECR1 single nucleotide polymorphisms selected from the group consisting of CBFA2T1 SNP1, CBFA2T1 SNP2, CBFA2T1 SNP3, CBFA2T1 SNP4, DECR1 SNP5, DECR1 SNP6, DECR1 SNP7, DECR1 SNP8, DECR1 SNP9, DECR1 SNP10, DECR1 SNP11, DECR1 SNP12 and DECR1 SNP13, which may provide novel markers for carcass quality, growth and/or feed efficiency. Additional aspects provide for novel methods which may comprise marker-assisted selection or marker-assisted management to improve carcass quality, growth and/or feed efficiency in cattle. | 05-07-2009 |
| 20090117557 | Rapid Detection of Microorganisms - Tools and methods for detecting the presence bacteria, yeast and mold in a sample obtained from a food sample are provided. The methods employ a polymerase chain reaction and primer and probe sets that are based on the 16S rRNA and squalene-hopene cyclase genes of | 05-07-2009 |
| 20090117558 | Method for improved specificity in probe based assays - Disclosed are compositions and methods for analyzing a target sequence in a sample. Generally, the method includes use of at least one pair of probes (Probe A and Probe B). In one embodiment, Probe A hybridizes to wanted and unwanted nucleic acid in the sample and bears a fluorophore and Probe B hybridizes to unwanted nucleic acid in the sample and bears a quencher. Fluorescence signal from Probe A hybridizing to unwanted nucleic acid is quenched by any relatively close hybridization of Probe B hereby increasing the specificity for the presence, amount or absence of the wanted target sequence. In preferred embodiments, the method is referred to as Fluorescence In-Situ Hybridization (FISH). The invention has many useful applications including rapidly detecting a microbial target sequence in a clinical sample. | 05-07-2009 |
| 20090117559 | Methods for Determining Probability of an Adverse or Favorable Reaction to a Niacin Receptor Agonist - The present invention relates generally to a GPR109A niacin receptor. The present invention relates more particularly to assessing a GPR109A polymorphism in an individual, wherein the GPR109A polymorphism is indicative of the subject's risk for an adverse reaction to the administration of a GPR109A receptor agonist, wherein the adverse reaction is associated with stimulation of MAP kinase activity by the GPR109A receptor agonist. More specifically, the present invention relates to assessing a GPR109A polymorphism in an individual and determining the level of risk for the subject for experiencing an adverse reaction, wherein the subject's GPR109A zygosity is predictive of the risk for a cutaneous flushing response that can be experienced following administration of a GPR109A receptor agonist. | 05-07-2009 |
| 20090117560 | Method of Forming Self Assembly Substance on Microsphere and Method of Detecting Target Analyte - It is intended to provide a method, in which the sensitivity in detecting a target analyte on a microsphere can be elevated and plural items can be detected at the same time. The presence of at least one target analyte in a sample is detected by bonding a microsphere having a fluorescent substance on the surface and the target analyte to a self assembly substance formed by using two oligonucleotide probes having complementary base sequence regions which are hybridizable with each other via the target analyte, thus forming a self assembly substance-bonded particle, and then analyzing the self assembly substance-bonded particle. | 05-07-2009 |
| 20090117561 | DIFFERENTIAL EXPRESSION GENE PROFILES AND APPLICATIONS IN MOLECULAR STAGING OF HUMAN GASTRIC CANCER - The invention provides methods for detecting differential gene expression in intestinal gastric tissue in a mammal by comparing the expression of specific genes in an intestinal gastric tissue suspected of being cancerous with that of the corresponding adjacent intestinal gastric tissue or a normal gastric mucosa tissue. The methods can be used in diagnosing or monitoring the progression of intestinal gastric cancer and determining the levels of differentiation of intestinal gastric cancer Systems and kits for methods of the invention are also provided. | 05-07-2009 |
| 20090117562 | Method and kit for diagnosing Autism using gene expression profiling - This invention relates to DNA microarray technology, and more specifically to methods and kits for identifying autism and autism spectrum disorders in humans. | 05-07-2009 |
| 20090117563 | Identification of TRPML3 (MCOLN3) as a Salty Taste Receptor and Use in Assays for Identifying Taste (Salty) Modulators and/or Therapeutics that Modulate Sodium Transport, Absorption or Excretion and/or Aldosterone and/or Vasopressin Production or Release - This invention relates to the elucidation that TRPML3 is involved in salty taste perception in primates including humans and likely other mammals (given the significance of sodium and other ions to physiological functions and conditions this phenotype is likely strongly conserved in different animals). The invention also relates to the discovery that the TRPML3 gene also modulates one or more of sodium metabolism, sodium excretion, blood pressure, fluid retention, cardiac function and urinary functions such as urine production and excretion. The invention also relates to transgenic animals that have been engineered to express or knock out TRPML3 expression and assays using TRPML3 expressing animals, cells and isolated ion channel polypeptides for identifying compounds that modulate TRPML3-associated functions including salty taste, sodium metabolism, sodium excretion, blood pressure, fluid retention, cardiac function and urinary functions such as urine production and excretion. | 05-07-2009 |
| 20090117564 | ESTROGEN RECEPTOR GENES AND UTILIZATION THEREOF - An estrogen receptor gene and utilization of the estrogen receptor gene. The estrogen receptor gene contains a nucleotide sequence coding for any of the following amino acid sequences: (a) the amino acid sequence of SEQ ID NO:1, (b) the amino acid sequence of SEQ ID NO:4, (c) the amino acid sequence of SEQ ID NO:23, (d) an amino acid sequence exhibiting 95% or more amino acid identity to the amino acid sequence of SEQ ID NO:1, (e) an amino acid sequence exhibiting 95% or more amino acid identity to the amino acid sequence of SEQ ID NO:4, and (f) an amino acid sequence exhibiting 85% or more amino acid identity to the amino acid sequence of SEQ ID NO:23. | 05-07-2009 |
| 20090117565 | Compositions and methods for diagnosis and treating mood disorders - The present invention provides methods for diagnosing mental disorders such as mood disorders, including bipolar disorder I and II and major depression; The invention also provides methods of identifying modulators of such mental disorders as well as methods of using these modulators to treat patients suffering from such mental disorders. | 05-07-2009 |
| 20090117566 | Compositions and methods for the diagnosis and treatment of tumor - The present invention is directed to compositions of matter useful for the diagnosis and treatment of tumor in mammals and to methods of using those compositions of matter for the same. | 05-07-2009 |
| 20090117567 | EUBACTERIAL tmRNA SEQUENCES AND USES THEREOF - The present invention is directed to eubacterial tmDNA sequences and the corresponding tmRNA sequences. The present invention is further directed to alignments of eubacterial tmDNA sequences and the use of the sequences and sequence alignments for the development of antibacterial drugs. The present invention is also directed to the use of the sequences for the development of diagnostic assays. | 05-07-2009 |
| 20090117568 | EUBACTERIAL tmRNA SEQUENCES AND USES THEREOF - The present invention is directed to eubacterial tmDNA sequences and the corresponding tmRNA sequences. The present invention is further directed to alignments of eubacterial tmDNA sequences and the use of the sequences and sequence alignments for the development of antibacterial drugs. The present invention is also directed to the use of the sequences for the development of diagnostic assays. | 05-07-2009 |
| 20090117569 | EUBACTERIAL tmRNA SEQUENCES AND USES THEREOF - The present invention is directed to eubacterial tmDNA sequences and the corresponding tmRNA sequences. The present invention is further directed to alignments of eubacterial tmDNA sequences and the use of the sequences and sequence alignments for the development of antibacterial drugs. The present invention is also directed to the use of the sequences for the development of diagnostic assays. | 05-07-2009 |
| 20090117570 | EUBACTERIAL tmRNA SEQUENCES AND USES THEREOF - The present invention is directed to eubacterial tmDNA sequences and the corresponding tmRNA sequences. The present invention is further directed to alignments of eubacterial tmDNA sequences and the use of the sequences and sequence alignments for the development of antibacterial drugs. The present invention is also directed to the use of the sequences for the development of diagnostic assays. | 05-07-2009 |
| 20090117571 | IMPEDANCE SPECTROSCOPY OF BIOMOLECULES USING FUNCTIONALIZED NANOPARTICLES - A biosensor system includes a functionalized interdigitated electrode, functionalized nanoparticles, a current/voltage signal generator, and a circuit analyzer. The interdigitated electrode can be functionalized by coating an exposed surface with first biomolecular probes. The nanoparticles are functionalized by coating an outer surface with second biomolecular probes. A signal generator provides a signal (e.g., an alternating current or voltage) having a selected range of frequencies. A circuit analyzer analyzes electrical parameters of the circuit as the signal is applied. Sensitivity is increased by the presence of functionalized nanoparticles in the system. An analytic method includes measuring changes in electrical parameters of the circuit over the range of frequencies. Using these measurements, the biosensor system can determine whether a target biomolecule is bound. The biosensor system can also identify a biomolecule by comparing the detected signal or “electro-fingerprint” with a reference set of signals over the frequency range. | 05-07-2009 |
| 20090117572 | Cell-Based Fluorescence Resonance Energy Transfer (FRET) Assays For Clostridial Toxins - The present invention provides a method of determining clostridial toxin activity by (a) contacting with a sample a cell containing a clostridial toxin substrate that includes a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence containing a cleavage site that intervenes between the donor fluorophore and the acceptor, where resonance energy transfer is exhibited between the donor fluorophore and the acceptor under the appropriate conditions; (b) exciting the donor fluorophore; and (c) determining resonance energy transfer of the contacted cell relative to a control cell, where a difference in resonance energy transfer of the contacted cell as compared to the control cell is indicative of clostridial toxin activity. | 05-07-2009 |
| 20090117573 | Locus specific amplification using array probes - Methods are provided for multiplexed amplification of selected targets and analysis of the amplified targets. In preferred aspects the amplification and analysis take place on the same solid support and preferably in a localized area such as a bead or a feature of an array. In preferred aspects the analysis is a determination of sequence at one or more locations in the amplified target. The methods may be used for genotyping, sequencing and analysis of copy number. | 05-07-2009 |
| 20090117574 | SELF-ACTUATING SIGNAL PRODUCING DETECTION DEVICES AND METHODS - An assay system is provided of great sensitivity and portability where the presence of a specific target in a sample, as well as its concentration (qualification and quantification) is detected by reason of a potential or voltage in a closed circuit, built up a redox reaction. The reaction is produced by binding a capture moiety to an enzymatic redox reaction partner, allowing the capture moiety to bind to any target in the sample, and washing any such bound target. The bound target, if not immobilized, may be immobilized through use of a second capture moiety. Substrate for the enzyme is then added. The action of the enzyme upon the substrate frees electrons, creating a potential across an anode and cathode which may be separated by a membrane. | 05-07-2009 |
| 20090117575 | Detection Probe Acting by Molecular Recognition - The present invention relates to a detection probe acting by molecular recognition of a target sequence, comprising successively in the 5′-3′ direction:
| 05-07-2009 |
| 20090117576 | METHODS FOR ANALYZING NUCLEIC ACID - The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification). | 05-07-2009 |
| 20090117577 | METHODS TO IDENTIFY COMPOUNDS USEFUL FOR TUMOR SENSITIZATION TO DNA DAMAGE - Cdc25A is herein identified as a substrate for β-TrCP1- or β-TrCP2-mediated ubiquitination and subsequent degradation via the ubiquitin-proteasome pathway. In particular, it has been found that interfering with β-TrCP expression or function, or increasing β-TrCP degradation, leads to accumulation of Cdc25A in a cell. Since degradation of Cdc25A is a key feature of the response to DNA damage, leading to a stall in the cell cycle during which the cell can repair the damage, Cdc25A accumulation can abolish this response, thereby sensitizing the cell to DNA damage. Described herein are assays for identifying β-TrCP inhibitors, and method of using such inhibitors for modulating Cdc25A degradation, sensitization of tumor cells, and as adjuvants in cancer therapy based on DNA damaging agents. | 05-07-2009 |
| 20090117578 | Method for identifying type I diabetes mellitus in humans - A method and system for classifying subject populations utilizing predictive and diagnostic biomarkers for type I diabetes mellitus. The method including determining the levels of a variety of markers within the serum or plasma of a target organism and correlating this level to general populations as a screen for predisposition or progressive monitoring of disease presence or predisposition. | 05-07-2009 |
| 20090117579 | Relating To The Handling Of DNA - A variety of methods are provided which use a silicon or silicon dioxide channel to extract DNA from a sample and then release it at a later point. The extraction channels are simple to manufacture and reliable in use. Prior art problems with entrainment of gas, liquid and solid material within channels are addressed. The techniques provide a convenient way of controlling the amount or concentration of DNA in the eluant. | 05-07-2009 |
| 20090123911 | ISOLATED STAPHYLOCOCCUS DNAX SUBUNIT AND USE THEREOF - The duplex DNA of chromosomes is replicated in a multicomponent process. A helicase unwinds the DNA, a replicase synthesizes new DNA, and primase repeatedly synthesizes new primed starts on the lagging strand. The present invention is directed to the genes from Gram positive bacterium encoding these proteins, and their characterization. | 05-14-2009 |
| 20090123912 | Methods for quantitating small RNA molecules - In one aspect, the present invention provides methods for amplifying a microRNA molecule to produce DNA molecules. The methods each include the steps of: (a) using primer extension to make a DNA molecule that is complementary to a target microRNA molecule; and (b) using a universal forward primer and a reverse primer to amplify the DNA molecule to produce amplified DNA molecules. In some embodiments of the method, at least one of the forward primer and the reverse primer comprise at least one locked nucleic acid molecule. | 05-14-2009 |
| 20090123913 | Detection of nucleic acid differences using endonuclease cleavage/ligase resealing reactions and capillary electrophoresis or microarrays - The present invention is directed to various methods for detecting DNA sequence differences, including single nucleotide mutations or polymorphisms, one or more nucleotide insertions, and one or more nucleotide deletions. Labeled heteroduplex PCR fragments containing base mismatches are prepared. Endonuclease cleaves the heteroduplex PCR fragments both at the position containing the variation (one or more mismatched bases) and, to a lesser extent, at non-variant (perfectly matched) positions. Ligation of the cleavage products with a DNA ligase corrects non-variant cleavages and thus substantially reduces background. This is then followed by a detection step in which the reaction products are detected, and the position of the sequence variations are determined. | 05-14-2009 |
| 20090123914 | Genomic Assay - A method of detecting a nucleic acid sequence in a genomic sample, includes: providing the genomic sample containing a target nucleic acid sequence of a duplex nucleic acid; providing, a probe containing a probe nucleic acid sequence; providing a hybridization mixture containing the genomic sample, the probe, a hybridization promoting agent and labels; incubating the hybridization mixture; irradiating the incubated mixture with radiation effective to stimulate at least some of the labels to emit energy; and detecting from a fluorescent signal whether the probe perfectly matches the target nucleic acid sequence, wherein the detecting is completed within sixty minutes of the hybridization mixture providing, and the method is conducted without denaturing and without PCR amplifying the duplex nucleic acid. A kit for practicing the method includes the probe, the hybridization promoting agent, and labels. | 05-14-2009 |
| 20090123915 | HIGH THROUGHPUT METHODS COMPRISING ANALYSIS OF REPETITIVE ELEMENT DNA METHYLATION - Preferred aspects provide novel high-throughput, sensitive methods (e.g., real-time PCR-based (MethyLight™) reactions) for detection and/or measurement of global genomic 5-methylcytosine content, based on measurement of DNA methylation of Alu, LINE-1 repetitive sequences, and the chromosome 1 centromeric satellite alpha and juxtacentromeric satellite 2 repeat sequences. Additional aspects provide sensitive methods for determining the amount of a DNA (e.g., in formalin-fixed, paraffin-embedded tissues). Combined (mean) use of Alu and Sat2 repeat methylation measurements provides for a surprisingly close correlation with global genomic 5-methylcytosine content measurements obtained by HPLC. Methylation of Alu repeats was determined to be closely associated with HPLC-based global methylation levels, as was methylation of satellite 2 and LINE-1 global genomic 5-methylcytosine content. The assays provide surrogate markers for global genomic 5-methylcytosine content analyses, and have substantial utility for high-throughput and population-based studies (e.g., genetic and dietary influences on global DNA methylation, folate deficiency, MTHFR gene polymorphisms, etc). | 05-14-2009 |
| 20090123916 | Nucleic Acid Fragments and Specific Detection Method By Molecular Identification of Different Bacteria Species of the Genus Acinetobacter - The present invention relates to complete rpoB genes, rpoB gene fragments SEQ. ID. no 9 to 32 and fragments of hypervariable sequences of said rpoB genes SEQ. ID. no 33 to 56 and 77 to 100, and to nucleic acid fragments of non-coding hypervariable sequences flanking the rpoB gene of sequences SEQ. ID. no 121 to 144 and 165 to 188, and also to oligonucleotides of species-specific sequences taken from said fragments of hypervariable sequences and to consensus oligonucleotides between the different species of | 05-14-2009 |
| 20090123917 | METHODS FOR QUANTIFYING MICRORNA PRECURSORS - Provided herein is a sensitive, high throughput, real-time PCR assay to monitor the expression of miRNA precursors. Gene specific primers and reverse transcriptase were used to convert the primary precursors and pre-miRNAs to cDNA. The expression of 23 miRNA precursors in six human cancer cell lines was assayed using the PCR assay. The miRNA precursors accumulated to different levels when compared to each other or when a single precursor is compared in the various cell lines. The precursor expression profile of three miRNAs determined by the PCR assay was identical to the mature miRNA expression profile determined by Northern blotting. We propose that the PCR assay may be scaled up to include all of the 150+ known human miRNA genes and can easily be adaptable to other organisms such as plants, | 05-14-2009 |
| 20090123918 | Human Protooncogene TRG and Protein Encoded Therein - Disclosed are a protooncogene and a protein encoded by the same. The protooncogene of the present invention, known to be involved in human carcinogenesis, may be effectively used for diagnosing various cancers including uterine cancer, leukemia, lymphoma, colon cancer, lung cancer, skin cancer, etc. | 05-14-2009 |
| 20090123919 | Diagnosing Pathological Conditions Using Interallelic Epigenetic Variations - The present invention is of interallelic epigenetic alterations in cells of cancer-stricken individuals which can be used for diagnosing cancer or cancer risk. In addition, the present invention is of interallelic epigenetic pattern alterations and/or interallelic replication pattern alterations in cells of a conceptus having imbalanced chromosome(s) or in maternal cells of the pregnant female carrying the conceptus, which can be used for prenatal diagnosis of chromosomal imbalances in the conceptus. Moreover, the present invention is of interallelic epigenetic pattern alterations and/or interallelic replication pattern alterations in cells of individuals having a chromosomal imbalance mosaicism which can be used for diagnosing chromosomal imbalance mosaicism, either prenatally or after birth. | 05-14-2009 |
| 20090123920 | JAK2 MUTATIONS - The invention disclosed herein is based on the identification of novel mutations in the JAK2 gene and JAK2 protein. The invention provides methods and compositions useful for diagnosing neoplastic diseases including, for example, myeloproliferative diseases. The invention also provides methods and compositions useful for determining a prognosis of an individual diagnosed as having a neoplastic disease. | 05-14-2009 |
| 20090123921 | IMMUNOGLOBULIN LIBRARIES - Methods and compositions for the screening and isolation of ligand-binding polypeptides, such as antibodies. In some aspects, methods of the invention enable the isolation of intact soluble antibodies comprising a constant domain. Screening methods that employ genetic packages such as bacteria and bacteriophages enable high through-put identification of ligand binding molecules. | 05-14-2009 |
| 20090123922 | Bead Bound Combinatorial Oligonucleoside Phosphorothioate And Phosphorodithioate Aptamer Libraries - The present invention includes composition and methods for making and using a combinatorial library having two or more beads, wherein attached to each bead is a unique nucleic acid aptamer that have disposed thereon a unique sequence. The library aptamers may be attached covalently to the one or more beads, which may be polystyrene beads. The aptamers may include phosphorothioate, phosphorodithioate and/or methylphosphonate linkages and may be single or double stranded DNA, RNA or even PNAs. | 05-14-2009 |
| 20090123923 | Method for obtaining information regarding quantity of DNA after non-methylated cytosine converting treatment in analysis of DNA methylation - The present invention provides an easy and accurate method for obtaining information regarding a quantity of DNA included in a sample used for analyzing a methylation of a target DNA, comprising steps of: treating a biological sample containing DNA including the target DNA with a non-methylated cytosine converting agent for converting non-methylated cytosine of the DNA into another base; and obtaining information regarding a quantity of DNA included in the sample treated in the treating step by using an oligonucleotide complementary to a cytosine-free region of the target DNA. | 05-14-2009 |
| 20090123924 | Method for Breast Cancer Diagnosis - The invention relates to a method for the in vitro diagnosis of breast cancer in a patient who may be suffering from a breast cancer, characterized in that it comprises the following steps:
| 05-14-2009 |
| 20090123925 | CANCER THERAPY PROGNOSIS AND TARGET - The present invention relates to a gene and/or protein expression based method of predicting response to platinum based chemotherapy for lung cancer patients, as well as a method of predicting prognosis of survival based on protein expression and type of lung cancer. The invention also provides novel targets for screening candidate anti-cancer agents. | 05-14-2009 |
| 20090123926 | Method for the diagnosis of genetic diseases by molecular combing and diagnostic kit - The invention concerns a method for detecting or locating one or several genes of one or several specific A DNA sequence or one or several molecules reacting with DNA on a B DNA characterised in that it consists in: (a) fixing and combing a certain amount of said B DNA on a combing surface; (b) reacting the product of the B combing with one or several probes, linked with the gene(s) or specific A DNA sequences, or with the molecules capable of reacting with DNA; (c) extracting information corresponding to at least one of the following categories: (1) the position of the probes. (2) the distance between the probes, (3) the size of the probes (the total sum of sizes for quantifying the number of hybridised probes) for determining therefrom the presence, the location and/or the amount of genes or specific A DNA sequences. This method can be used in particular for the diagnosis of genetic diseases. | 05-14-2009 |
| 20090123927 | ISOLATION OF PRECURSOR CELLS AND THEIR USE FOR TISSUE REPAIR - Cartilage-derived morphogenetic protein CDMP-1 or a transforming growth factor β having at least 80% homology with CDMP-1, or a factor co-expressed and/or co-detectable therewith, is used as a marker of skeletal precursor cells from any part of a mammalian body. | 05-14-2009 |
| 20090123928 | Genomic Landscapes of Human Breast and Colorectal Cancers - Human cancer is caused by the accumulation of mutations in oncogenes and tumor suppressor genes. To catalogue the genetic changes that occur during tumorigenesis, we isolated DNA from 11 breast and 11 colorectal tumors and determined the sequences of the genes in the Reference Sequence database in these samples. Based on analysis of exons representing 20,857 transcripts from 18,191 genes, we conclude that the genomic landscapes of breast and colorectal cancers are composed of a handful of commonly mutated gene “mountains” and a much larger number of gene “hills” that are mutated at low frequency. We describe statistical and bioinformatic tools that may help identify mutations with a role in tumorigenesis. These results have implications for understanding the nature and heterogeneity of human cancers and for using personal genomics for tumor diagnosis and therapy. | 05-14-2009 |
| 20090123929 | Methods and kits for diagnosing or monitoring autoimmune and chronic inflammatory diseases - The present invention relates to compositions and methods for diagnosing, monitoring and/or treating an autoimmune or chronic inflammatory disease. In particular, the present invention provides methods for diagnosing, monitoring and treating an autoimmune disease (e.g., rheumatoid arthritis) or chronic inflammatory disease (e.g., systemic lupus erythematosus) based on detecting or altering (e.g., altering expression or methylation status of) autoimmune or chronic inflammatory disease proteins (e.g., CD70 and CD40L). The present invention also provides kits for detecting methylation status of autoimmune or chronic inflammatory disease proteins (e.g., CD70 and CD40L) and for diagnosing, monitoring and/or treating autoimmune or chronic inflammatory diseases. | 05-14-2009 |
| 20090123930 | METHODS AND COMPOSITIONS FOR DIAGNOSTIC USE IN CANCER PATIENTS - Disclosed herein are methods and compositions useful for identifying therapies likely to confer optimal clinical benefit for patients with cancer. | 05-14-2009 |
| 20090123931 | IDENTIFICATION OF TISSUE FOR DEBRIDEMENT - Provided are methods of determining whether a cell in a tissue site is viable or nonviable. Also provided are methods of debriding tissue from a tissue site. Further provided are kits comprising a compound that distinguishes between viable and nonviable cells and instructions for using the compound on a tissue site. Additionally, the use of a compound that distinguishes between viable and nonviable cells is provided, where the use is to determine whether a cell in a tissue site is viable or nonviable. Also provided is a use of a compound that distinguishes between viable and nonviable cells, where the use is for the manufacture of the above-described kit. | 05-14-2009 |
| 20090123932 | QUANTITATIVE TEST TO DETECT DISEASE PROGRESSION MARKERS OF EPITHELIAL OVARIAN CANCER PATIENTS - The present invention concerns a method of prognosing the risk of early ovarian cancer relapse in a subject having ovarian cancer comprising: a) detecting the level of at least one marker selected from the group consisting of BTF4, GCS and HLA-DRbeta1; and b) comparing the level of the above at least one marker with that of a corresponding control sample, wherein the detection of a lower level of the at least one marker compared to that in the control sample is indicative that the subject is at risk of early cancer relapse. Also provided is a method of stratifying a subject suffering from ovarian cancer based on the expression levels of the disclosed markers and kits for practicing the methods of the present invention. | 05-14-2009 |
| 20090123933 | MICRORNA BIOMARKERS IN LUPUS - The present invention provides methods of screening a subject for systemic lupus erythematosus (SLE), comprising detecting an increase in an amount of one or more markers associated with SLE in a biological sample from the subject, wherein the one or more markers is selected from the group consisting of miR-16-1, miR-16-2, miR-223, let7a-1, let7a-2. let7a-3, let 7c, let7g, and any combination thereof, whereby detection of the increase in the amount of the one or more markers identifies the subject as having SLE. The invention further provides methods of screening a subject for SLE comprising detecting a decrease in miR-95 in a biological sample from the subject, whereby detection of the decrease in the amount of miR-95 identifies the subject as having SLE. | 05-14-2009 |
| 20090123934 | Inhibition of polo kinase by matrimony maintains G2 arrest in the meiotic cell cycle - Matrimony (Mtrm) acts as a negative regulator of Polo kinase (Polo) during the later stages of G2 arrest. Indeed, both the repression of Polo expression until stage 11 and the inactivation of newly synthesized Polo by Mtrm until stage 13 play critical roles in maintaining and properly terminating G2 arrest. Our data suggest a model in which the eventual activation of Cdc25 by an excess of Polo at stage 13 triggers NEB and entry into prometaphase. In view of the foregoing, methods for modulating oocyte maturation are provided. More particularly, methods are provided for in vitro maturation of an oocyte. Further provided are methods for identifying functional orthologs of a | 05-14-2009 |
| 20090123935 | Measurement of a population of nucleic acids, in particular by real time PCR - The invention relates to a method for measuring the amount of nucleic acids of a target sequence in a sample of interest. The method comprises several steps, and in particular subjecting the sample of interest to an amplification treatment, in the presence of at least one nucleic acids label specific for said target sequence; measuring a physical quantity representative of the evolution of the label; calculating a parameter F | 05-14-2009 |
| 20090130656 | NEISSERIA GONORRHOEAE DETECTION - A method for determining whether a human individual is or has been infected with | 05-21-2009 |
| 20090130657 | AMPLIFICATION BLOCKER COMPRISING INTERCALATING NUCLEIC ACIDS (INA) CONTAINING INTERCALATING PSEUDONUCLEOTIDES (IPN) - An amplification blocker comprising an intercalating nucleic acid (INA) containing two or more internal intercalating pseudonucleotides (IPNs) capable of blocking or reducing nucleic acid amplification. Use of the amplification blocker to block or reduce nucleic acid amplification. | 05-21-2009 |
| 20090130658 | Arrangement for integrated and automated dna or protein analysis in a single-use cartridge, method for producing such a cartridge and operating method for dna or protein analysis using such a cartridge - A cartridge (card) having a system of microchannels and/or microcavities is used for automated DNA or protein analysis. In at least one embodiment, the microchannels or microcavities include geometrical structures for receiving dry reagents. For the purpose of industrial production, the cartridge is produced from a flat card support, e.g., by injection moulding. The reagents are spotted into the open channels, dried and then the channels are sealed by way of a film. A finished cartridge can thus be provided with a test sample and the fully automated measuring sequence can be initiated by inserting said cartridge into a read-out device. | 05-21-2009 |
| 20090130659 | KITS AND METHODS FOR DETECTING METHYLATED DNA - The present invention relates to an in vitro method for detecting methylated DNA comprising (a) coating a container with a polypeptide capable of binding methylated DNA; (b) contacting said polypeptide with a sample comprising methylated and/or unmethylated DNA; and (c) detecting the binding of said polypeptide to methylated DNA. In a preferred embodiment, said method further comprises step (d) analyzing the detected methylated DNA by sequencing. Another aspect of the present invention is a kit for detecting methylated DNA according to the methods of the invention comprising (a) a polypeptide capable of binding methylated DNA; (b) a container which can be coated with said polypeptide; (c) means for coating said container; and (d) means for detecting methylated DNA. | 05-21-2009 |
| 20090130660 | Single Nucelotide Polymorphism (SNP) - Association of Type 2 diabetes with single nucleotide polymorphisms and haplotypes are disclosed. Also disclosed are diagnostic applications in identifying those who have Type 2 diabetes or are at risk of developing Type 2 diabetes, and discovery of therapeutic agents and methods of treatment. | 05-21-2009 |
| 20090130661 | Method for Detecting IL-16 Activity and Modulation of IL-16 Activity Based on Phosphorylated Stat-6 Proxy Levels - Methods for detecting IL-16 biological activity, detecting modulation of IL-16 biological activity, and diagnosing the presence of, or susceptibility to, an IL-16-related disorder in a subject involve measuring and comparing the levels of a phosphorylated STAT-6 proxy produced by eukaryotic cells expressing CD4 or CD9, peripheral blood mononuclear cells, HuT-78 cells, or THP-1 cells. | 05-21-2009 |
| 20090130662 | Method for Diagnosis of Prostate Cancer - The present invention provides a method for detecting and/or quantifying PCA-1 in a body fluid sample from a subject as a prostate cancer marker. The present invention also provides a method for detecting and/or quantifying an anti-PCA-1 autoantibody in a body fluid sample from a subject as a prostate cancer marker. The present invention allows diagnosis of prostate cancer to be performed more simply, more rapidly and at lower cost. | 05-21-2009 |
| 20090130663 | Hybridization Detection Method Using an Intercalator - To provide a novel and useful technique capable of achieving an improve S/N ratio in a hybridization detection technique using an intercalator. A method is provided wherein a hybridization is detected by measuring a fluorescence intensity from an intercalator I binding to a probe nucleic acid P and a complementary strand site of a target nucleic acid T. In the invention, a single-stranded target nucleic acid Ts, a single-stranded probe nucleic acid Ps forming no complementary strand and surplus nucleic acid moieties T | 05-21-2009 |
| 20090130664 | Methods and compositions for the identification of antibiotics that are not susceptible to antibiotic resistance in pseudomonas aeruginosa - The “instant evolution” system was initially developed in | 05-21-2009 |
| 20090130665 | POLYMER COMPOSITIONS AND USES THEREOF - Block copolymers labelled with molecular recognition units and comprising a hydrophobic block and a luminescent block are presented. A method of detecting biomolecules using such block copolymers is also presented. More specifically, the block copolymers of the present invention have the following Formula (I): wherein “A” is a hydrophobic block; “B” is a luminescent block; “C” is a hydrophilic block; “D” is a molecular recognition unit; “n” and “m” are integers ranging from 1 to 75; “x” is either 0 or an integer ranging from 1 to 75; and “Y” is either 0 or 1. | 05-21-2009 |
| 20090130666 | Cytometric system including nucleic acid sequence amplification, and method - The invention relates to a cytometric system that can include a first station capable of selectively sorting a nucleic acid sequence-containing biological material from a biological sample, and a second station where nucleic acid of the sorted nucleic acid sequence-containing biological material, can undergo a nucleic acid amplification reaction. The system can include a conduit system adapted to facilitate transfer of a sorted biological material or processed biological material from one region or station, to another. All of the stations can be controlled by a central control system. Methods of sorting a biological material from a biological sample, and methods of amplifying nucleic acid of a sorted nucleic acid sequence-containing material, are also provided. | 05-21-2009 |
| 20090130667 | PROBE, PROBE SET, PROBE-IMMOBILIZED CARRIER, AND GENETIC TESTING METHOD - A nucleic acid probe for classification of pathogenic bacterial species is capable of collectively detecting bacterial strains of the same species and differentially detecting them from other bacterial species. Any one of the base sequences of SEQ ID NOS. 81 to 83 and complementary or modified sequences thereof or a combination of at least two of them is used for detecting the gene of an infectious disease pathogenic bacterium. | 05-21-2009 |
| 20090130668 | PROBE, PROBE SET, PROBE-IMMOBILIZED CARRIER, AND GENETIC TESTING METHOD - A nucleic acid probe for classification of pathogenic bacterial species is capable of collectively detecting bacterial strains of the same species and differentially detecting them from other bacterial species. Any one of the base sequences of SEQ ID NOS. 78 to 80 and complementary or modified sequences thereof or a combination of at least two of them is used for detecting the gene of an infectious disease pathogenic bacterium. | 05-21-2009 |
| 20090130669 | PROBE, PROBE SET, PROBE-IMMOBILIZED CARRIER, AND GENETIC TESTING METHOD - A nucleic acid probe for classification of pathogenic bacterial species is capable of collectively detecting bacterial strains of the same species and differentially detecting them from other bacterial species. Any one of the base sequences of SEQ ID NOS. 92 to 93 and complementary or modified sequences thereof or a combination of at least two of them is used for detecting the gene of an infectious disease pathogenic bacterium. | 05-21-2009 |
| 20090130670 | PROBE, PROBE SET, PROBE-IMMOBILIZED CARRIER, AND GENETIC TESTING METHOD - A nucleic acid probe for classification of pathogenic bacterial species is capable of collectively detecting bacterial strains of the same species and differentially detecting them from other bacterial species. Any one of the base sequence of SEQ ID NO. 94 and complementary or modified sequences thereof or a combination of at least two of them is used for detecting the gene of an infectious disease pathogenic bacterium. | 05-21-2009 |
| 20090130671 | PROBE, PROBE SET, PROBE-IMMOBILIZED CARRIER, AND GENETIC TESTING METHOD - A nucleic acid probe for classification of pathogenic bacterial species is capable of collectively detecting bacterial strains of the same species and differentially detecting them from other bacterial species. Any one of the base sequences of SEQ ID NOS. 73 to 75 and complementary or modified sequences thereof or a combination of at least two of them is used for detecting the gene of an infectious disease pathogenic bacterium. | 05-21-2009 |
| 20090130672 | METHODS FOR IDENTIFYING NOVEL PESTICIDAL GENE HOMOLOGUES - Methods and compositions for identifying novel pesticidal gene homologues are provided. Specifically, the methods of the invention comprise systematically designing oligonucleotide primers that are specific for a pesticidal gene of interest and performing successive rounds of PCR amplification of nucleic acid material from a microorganism, particularly a | 05-21-2009 |
| 20090130673 | Methods for detecting and differentiating mycobacterium genus and mycobacterium avium complex in a sample or culture - The present invention relates to compositions and methods for detecting | 05-21-2009 |
| 20090130674 | Circular DNA molecule having a conditional origin of replication, process for their preparation and their use in gene therapy - A prokaryotic recombinant host cell comprising a heterologous replication initiation protein that activates a conditional origin of replication and an extrachromosomal DNA molecule comprising a heterologous therapeutic gene and a conditional origin of replication whose functionality in the prokaryotic recombinant host cell requires a replication initiating protein which is foreign to the host cell is described. The host cell may comprise a pir gene having at least one mutation, which may occur in the pir gene copy number control region, the pir gene leucine zipper-like motif, or the pir gene DNA binding region. | 05-21-2009 |
| 20090130675 | Genes Involved in the Biosynthesis of Thiocoraline and Heterologous Production of Same - The invention relates to genes involved in the biosynthesis of thiocoraline and to the heterologous production of same. According to the invention, the cluster of genes responsible for the biosynthesis of thiocoraline was identified and cloned. Said cluster of genes can be used in the heterologous production of thiocoraline which has an antitumor and antibacterial activity. | 05-21-2009 |
| 20090130676 | Interaction trap systems for detecting protein interactions - Disclosed herein is a method of determining whether a first protein is capable of physically interacting with a second protein, involving: (a) providing a host cell which contains (i) a reporter gene operably linked to a protein binding site; (ii) a first fusion gene which expresses a first fusion protein, the first fusion protein including the first protein covalently bonded to a binding moiety which is capable of specifically binding to the protein binding site; and (iii) a second fusion gene which expresses a second fusion protein, the second fusion protein including the second protein covalently bonded to a gene activating moiety and being conformationally-constrained; and (b) measuring expression of the reporter gene as a measure of an interaction between the first and the second proteins. Also disclosed are methods for assaying protein interactions, and identifying antagonists and agonists of protein interactions. Proteins isolated by these methods are also discussed. Finally, populations of eukaryotic cells are disclosed, each cell having a recombinant DNA molecule encoding a conformationally-constrained intracellular peptide. | 05-21-2009 |
| 20090130677 | METHOD FOR ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS AND METHOD FOR DETECTING NUCLEIC ACIDS USING SIMULTANEOUS ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS AND SIGNAL PROBE - The present invention relates to a method for isothermal amplification of nucleic acids and a method for detecting nucleic acids, which comprises characterized in simultaneous isothermal amplification of nucleic acids and a signal probe to a method for isothermal amplification of target nucleic acids using an external primer set and RNA/DNA hybrid primer set, and a method for detecting amplification products by amplifying nucleic acids and a signal probe simultaneously using an external primer set, RNA-DNA hybrid primer set and DNA-RNA-DNA hybrid probe. The method according to the present invention is convenient compared with the conventional method, it is possible to amplify the target nucleic acids rapidly and exactly without a risk of contamination, and it can simultaneously amplify a signal probe, so that it can be applied to various genome project, such as detection and identification of a pathogen, detection of gene modification causing predetermined phenotype, detection of hereditary diseases or determination of sensibility to diseases, estimation of gene expression and apply to genome project, thus being useful for molecular biological studies and disease diagnosis. | 05-21-2009 |
| 20090130678 | Methods and Kits for Breast Cancer Prognosis - The present invention relates to the field of prognosis of a proliferative disease in a patient. More specifically, the present invention relates to methods for the identification of the likely outcome of breast cancer in a breast cancer patient. The invention also relates to methods for the identification of the likely outcome of breast cancer in a patient on the basis of the ERBB2 status of the patient and expression levels of immune genes in tumour tissue of said patient. | 05-21-2009 |
| 20090130679 | Automated system and method for processing genetic material - The invention discloses an automated system and method for processing genetic material. Additionally, the automated system and method of the invention can extract a target genetic material from a sample; amplifying a target nucleic acid sequence from the genetic material; detecting the target nucleic acid by an optical detection module to qualify and/or quantify the target nucleic acid immediately. | 05-21-2009 |
| 20090130680 | SOLID SUPPORT - The present invention provides a solid support for performing steps of isolation of cell or extraction and purification of nucleic acid, safely, easily, efficiently, and with high yield in the genetic test for investigating the presence of pathogenic bacterial infection. A solid support for binding with cell as an embodiment of the above-described solid support, comprises a polypeptide having capability of binding with mycolic acid-containing glycolipid which is immobilized on the surface of a carrier. In addition, a solid support for binding with nucleic acid as another embodiment of the above-described solid support, comprises a polypeptide having capability of binding with nucleic acid which is immobilized on the surface of a carrier. | 05-21-2009 |
| 20090130681 | 21132, a Human G-protein coupled receptor family member and uses therefor - The invention provides isolated nucleic acids molecules, designated 21132 nucleic acid molecules, which encode novel GPCR family members. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing 21132 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a 21132 gene has been introduced or disrupted. The invention still further provides isolated 21132 proteins, fusion proteins, antigenic peptides and anti-21132 antibodies. Diagnostic and therapeutic methods utilizing compositions of the invention are also provided. | 05-21-2009 |
| 20090130682 | GENETIC POLYMORPHISMS ASSOCIATED WITH LIVER FIBROSIS, METHODS OF DETECTION AND USES THEREOF - The present invention is based on the discovery of genetic polymorphisms that are associated with liver fibrosis and related pathologies. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, including groups of nucleic acid molecules that may be used as a signature marker set, variant proteins encoded by such nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and proteins, and methods of using the nucleic acid and proteins as well as methods of using reagents for their detection. | 05-21-2009 |
| 20090130683 | PREDICTING AND DIAGNOSING PATIENTS WITH AUTOIMMUNE DISEASE - The present invention provides methods for the prediction and diagnosis of autoimmune diseases, including Systemic Lupus Erythematosus, using single nucleotide polymorphism in TNFAIP3 (A20). | 05-21-2009 |
| 20090130684 | METHODS OF DETECTING AND TREATMENT OF CANCERS USING SCUTELLARIA BARBATA EXTRACT - An extract of | 05-21-2009 |
| 20090130685 | Plants With Altered Root Architecture, Related Constructs and Methods Involving Genes Encoding Leucine Rich Repeat Kinase (LLRK) Polypeptides and Homologs Thereof - Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture. | 05-21-2009 |
| 20090130686 | Method for the Microscopic Localization of a Selected, Intracellular DNA Segment with a Known Nucleotide Sequence - The method for the microscopic localization in situ of a selected intracellular native genome segment with a known nucleotide sequence is characterized by the nature and the sequence of the following measures: (1.) The target DNA is analyzed, via genome databases, for partial sequences which constitute a unique pattern within the genome. (2.) Single-stranded probe sequences are provided which are identical to these partial sequences or complementary thereto, and which are suitable for hybridizing with the single strands of these subsequences via a Watson-Crick binding. (3.) The probe sequences are coupled with marker molecules, where all units of probe sequence and marker molecule(s) have the same binding behavior or the same melting point as the single strand of the target DNA complementary thereto. (4.) The probe sequences are introduced into the cell and combined with the target DNA so that they hybridize to the corresponding partial sequences, of the target DNA, which are temporarily present as two single strands. (5.) The marker signals emitted are detected, and (6.) the locus of the target DNA on the genome is identified on the basis of the presence and/or intensity and/or the simultaneous occurrence of different marker signals. | 05-21-2009 |
| 20090130687 | FORMULATIONS AND METHOD ISOLATING NUCLEIC ACIDS FROM ARBITRARY COMPLEX STARTING MATERIALS AND SUBSEQUENT COMPLEX GENETIC MATERIALS - The object of the invention is formulations and methods without chaotropic components for the isolation of nucleic acids with binding to a solid phase, in particular of DNA, from arbitrary complex starting materials containing a lysis/binding buffer system manifesting at least one anti-chaotropic salt component, the concentration of the anti-chaotropic salt components being between 0.001 mM and 0.1 M, preferably 0.1 mM, and further a solid phase and washing and elution buffers which are known per se. | 05-21-2009 |
| 20090130688 | Method for Accessing Microbial Diversity - A method of interfering with quorum sensing regulation of genes to promote cell growth is disclosed. The method of is aimed at accessing microbial biodiversity. The method involves obtaining an environmental sample comprising at least one novel (uncultivated in the laboratory) microorganism, contacting the environmental sample with an effective amount of an agent or combination of agents which interferes with the quorum sensing regulation of genes, growing the treated sample in a culture medium containing the quorum sensing signal disrupting agent or agents, and analyzing the colonies of microorganisms grown to demonstrate genetic novelty. | 05-21-2009 |
| 20090136918 | QUANTIFICATION OF MICROSPHERE SUSPENSION HYBRIDIZATION AND USES THEREOF - A novel suspension hybridization assay was used to determine nucleic acid copy number by flow cytometry. The assay was validated with low copy (lc) products ranging in length from 100 to 2304 bp conjugated to spectrally-distinct polystyrene microspheres. In the example provided herein, these conjugated microspheres were used as multiplex hybridization probes to detect homologous sequences in genomic DNA extracted from cytogenetic cell pellets and labeled with biotin-dUTP. Hybridization was detected with phycoerythrin-labeled streptavidin and analyzed by flow cytometry. Copy number differences were distinguishable by comparing the mean fluorescence intensities of test probes with a diploid reference probe in genomic DNA of patient samples and abnormal cell lines. The assay is capable of distinguishing a single allele and three alleles at a test locus from a biallelic reference sequence, regardless of chromosomal context. The assay is an improvement on previous methods which require prior amplification of locus-specific target DNA because, lc probes provide adequate specificity and sensitivity for accurate copy number determination of homologous targets. Because of its high sensitivity and accuracy, the assay is useful for determination of nucleic acid copy number for a variety of applications, including determination of genomic copy number in humans, animal models of disease and in solution, measurement of transcript levels, forensic DNA analysis, and quality control analysis in agriculture. | 05-28-2009 |
| 20090136919 | METHOD TO RAPIDLY QUANTIFY MYCOBACTERIA IN INDUSTRIAL FLUIDS - The invention is a method for detecting and quantifying environmental mycobacteria in industrial fluids comprising extracting bacterial DNA from a sample of an industrial fluid; amplifying the extracted DNA; hybridizing an aliquot of the DNA to two or more amplification primers; and detecting the amplification product. | 05-28-2009 |
| 20090136920 | Diagnostics and therapeutics for diseases associated with serum/glucocorticoid regulated kinase 1 (sgk1) - The invention provides a human SGK1 which is associated with cardiovascular diseases, cancer, endocrinological diseases, metabolic diseases, inflammation, gastroenterological diseases, hematological diseases, respiratory diseases, neurological diseases and urological diseases. The invention also provides assays for the identification of compounds useful in the treatment or prevention of cardiovascular diseases, cancer, endocrinological diseases, metabolic diseases, inflammation, gastroenterological diseases, hematological diseases, respiratory diseases, neurological diseases and urological diseases. The invention also features compounds which bind to and/or activate or inhibit the activity of SGK1 as well as pharmaceutical compositions comprising such compounds. | 05-28-2009 |
| 20090136921 | Association of Breast Cancer DNA Methylation Profiles with Hormone Receptor Status and Response to Tamoxifen - Particular embodiments provide novel and clinically useful DNA methylation predictors of hormone receptor status, and predictors of response to endocrine (e.g., hormonal) and non-endocrine breast cancer therapy. The ESR1 gene, encoding the estrogen receptor (ER) alpha proved to be the preferred predictor of progesterone receptor (PR) status, while methylation of the PGR gene, encoding PR, was the preferred predictor of ER status. ESR1 methylation outperformed hormone receptor status as a predictor of clinical response in patients treated with antiestroges (e.g., tamoxifen), while promoter methylation of the CYP1B1 gene, encoding a tamoxifen and estradiol metabolizing cytochrome P450, predicted response differentially in tamoxifen-treated and non-treated patients. High levels of promoter methylation of the ARHI gene, encoding a RAS-related small G-protein, were shown to be preferred predictors of better survival in patients who had not received tamoxifen therapy. | 05-28-2009 |
| 20090136922 | Method for carrying out an electrochemical measurement on a liquid measuring sample in a measuring chamber that can be accessed by lines, and corresponding arrangement - Especially in order to carry out the so-called enzyme-coupled DNA hybridisation test in a closed cartridge including a microfluid system, using stored dry reagents, the reagents must be dissolved in the microfluid system and transported into the measuring chamber directly before the measurement. During the dissolution of the reagents in water, air cushions that cannot reach the measuring chamber must absolutely be prevented from forming upstream of the reagent liquid. According to an embodiment of the invention, the liquid measuring sample and the liquid reagents are transported in such a way that the air cushion is directed into the waste line and the measuring sample and the reagents are then introduced into the measuring chamber without any air bubbles. In this way, measuring errors can be avoided. | 05-28-2009 |
| 20090136923 | PRIMER COMPOSITION FOR DETECTING MYCOBACTERIUM SP. AND THE DETECTION METHOD - Disclosed are a primer composition including two pairs of primers targeting one gene (mpb64) concerning Mycobacterium sp., and a pair of primers targeting a human-derived gene (HLA-DR); and a method for detecting Mycobacterium sp. using a multiplex one-tube nested PCR method in which their primers are used to amplify two genes in one tube at the same time. The method of the present invention may provide a useful guide post capable of clinically diagnosing Mycobacterium sp. in a specimen in a more specific, rapid and convenient manner. | 05-28-2009 |
| 20090136924 | RAPID GENERATION OF LONG SYNTHETIC CENTROMERIC TANDEM REPEATS FOR MAMMALIAN ARTIFICIAL CHROMOSOME FORMATION - Methods are described for construction of long synthetic arrays of DNA repeats, such as alphoid repeats or other repeat sequences. The methods include concatamerization of DNA into short repeats (for instance using rolling circle amplification or directional in vitro ligation), followed by assembling the short repeats into long arrays by homologous recombination during transformation into microbe cells. These methods can be described generally as Recombinational Amplification of Repeats (RAR). The long arrays are engineered centromere-like regions that allow one to construct mammalian artificial chromosomes with a predefined centromeric region structure. Artificial chromosomes, including human artificial chromosomes with a regulated centromere, and methods of their use are also provided | 05-28-2009 |
| 20090136925 | IDENTIFICATION OF TERPENOID-BIOSYNTHESIS RELATED REGULATORY PROTEIN-REGULATORY REGION ASSOCIATIONS - Materials and methods for identifying terpenoid regulatory region-regulatory protein associations are disclosed. Materials and methods for modulating expression of a sequence interest are also disclosed. | 05-28-2009 |
| 20090136926 | Device and method for standardizing nucleic acid concentrations - The present invention relates to a device and a method for normalising nucleic acid concentrations, preferably for normalising nucleic acid concentrations in enzymatic nucleic acid amplification and modification methods. | 05-28-2009 |
| 20090136927 | RANDOM HOMOZYGOUS GENE PERTURBATION TO ENHANCE ANTIBODY PRODUCTION - The invention reflects enhanced antibody expression of an antibody of interest by cell lines transformed by random homozygous gene perturbation methods to either increase or decrease the expression pattern of a gene of the cell line other than the antibody of interest. The transformed cell line exhibits specific productivity rates, SPR, for the RHGP transformed cell liens of 1.5 or more, as compared with the antibody expressing cell line parents prior to transformation by RHGP. A knock out or anti-sense construct may be devised to reduce expression of the target gene, a promoter may be inserter to enhance expression of the target gene. The antibodies expressed by the transformed cell lines exhibit the binding properties of their parent cell lines prior to transformation with RHGP, and increase Total Volumetric Production of said antibody by said cells in a given volume. | 05-28-2009 |
| 20090136928 | Compositions and Methods for the Diagnosis and Treatment of Tumor - The present invention is directed to compositions of matter useful for the diagnosis and treatment of tumor in mammals and to methods of using those compositions of matter for the same. | 05-28-2009 |
| 20090136929 | NOVEL IN VITRO METHOD OF QUANTIFYING DEMINERALIZED BONE OSTEOINDUCTIVITY - The present invention provides an in vitro method for determining the osteoinductive potential of a biomaterial. In particular, the method measures the expression of osterix by osteoblast progenitor cells incubated with the biomaterial. In various embodiments, the biomaterial is demineralized bone (DMB) and the progenitor cells are incubated with DMB or proteins extracted from DMB. | 05-28-2009 |
| 20090136930 | METHOD FOR THE IDENTIFICATION OF MICROORGANISMS BY MEANS OF IN SITU HYBRIDIZATION AND FLOW CYTOMETRY - The invention relates to a combined method for specifically identifying microorganisms by means of in situ hybridization and flow cytometry. The inventive method is particularly characterized by an improved specificity and a shorter duration of the process as opposed to methods known in prior art. | 05-28-2009 |
| 20090136931 | Novel fluorescent labeling compound - A rare-earth fluorescent complex which forms a fluorescent complex with two or more rare-earth metals such as europium and terbium and can effectively be excited at a wavelength of 340 nm or longer; a fluorescent labeling agent comprising the rare-earth fluorescent complex; a method of fluorescent labeling in which the rare-earth fluorescent complex is used as a labeling agent; and a method of fluorometric analysis in which the fluorescent labeling agent is used. The rare-earth fluorescent complex is characterized by having a cyclic ligand comprising a 4-biphenyl-2,2′:6′,2″-terpyridine skeleton and a 2,6-bis(3′-aminomethyl-1′-pyrazolyl)pyrazine skeleton bonded thereto. | 05-28-2009 |
| 20090136932 | FIBERS WITH ISOLATED BIOMOLECULES AND USES THEREOF - The present invention relates to compositions, methods, and uses for isolated biomolecule-containing fibers. The invention also relates to isolated, elongated biopolymers such as nucleic acids, polypeptides, lipids, and carbohydrates within fibers. The invention relates to methods of detecting and analyzing biomolecules in fibers using light, electrons, and neutrons. The invention further relates to methods of determining the sequence, structure, and properties of isolated, elongated biopolymers fixed within fibers. | 05-28-2009 |
| 20090136933 | Method for Determination of Amount of Double-Stranded DNA and Kit for the Determination - A method and kit for electrochemically simply determining with excellent precision the amount of double-stranded DNA does not require an expensive measurement electrode, such as an immobilized enzyme electrode or any high level electrode production technique which can retain uniform surface area accuracy. A method and kit are provided for electrochemically determining the amount of double-stranded DNA in a sample solution based on a residual amount of a substance capable of binding to the double-stranded DNA which is added to the solution in excess amount, in which a buffering substance is added to a sample solution, the buffering substance being capable of allowing an oxidation wave potential of the potential-current curve for the substance capable of binding to the double-stranded-DNA determined in a solution containing the buffering substance to change depending on the concentration of the free substance capable of binding to the double-stranded DNA in the solution. | 05-28-2009 |
| 20090136934 | TARGETING AND TRACING OF ANTIGENS IN LIVING CELLS - The present invention relates to a method of detecting the presence, amount or subcellular location of an antigenic structure of interest in a cell, comprising the steps of: (a) (i) expressing a fusion protein directed to the antigenic structure of interest in said cell or (ii) introducing a fusion protein directed to the antigenic structure of interest and coupled to a (poly) peptide capable of transducing into said cell; wherein said fusion protein comprises a first (poly) peptide sequence comprising the variable region of a heavy chain antibody of Camelidae and a second (poly) peptide sequence derivable from a fluorescent or chromophoric protein. | 05-28-2009 |
| 20090136935 | Dye Compounds - The present disclosure provides compounds that may be used as chromogens, fluorochromes, or as both. Said compounds may further be used as substrate for at least one enzyme, wherein said enzyme is capable of processing an aromatic amine group (—NH | 05-28-2009 |
| 20090136936 | IMMUNOGLOBULIN FC LIBRARIES - Methods and composition for the screening and isolation of aglycosylated antibody Fc domain polypeptides. For example, in certain aspects methods for identifying aglycosylated Fc domains that bind to Fc receptors or preferentially bind to particular Fc receptors are described. Furthermore, the invention provides aglycosylated Fc domains that bind to Fc receptors with high affinity. Enhanced methods and media for prokaryotic based interaction screening are also provided. | 05-28-2009 |
| 20090136937 | METHODS AND SYSTEMS FOR MONITORING PRODUCTION OF A TARGET PROTEIN IN A NANOLIPOPROTEIN PARTICLE - Provided herein are methods and systems for the monitoring production of a target protein in of a nanolipoprotein particle (NLP) that also includes a scaffold protein and a membrane forming lipid. The target protein is capable of assuming an active form and an inactive form. Monitoring is performed by an indicator protein that is capable of assuming an active form and an inactive form, the active form associated with a detectable activity of the indicator protein, the detectable activity further associated with the active form of the target protein. | 05-28-2009 |
| 20090136938 | METHODS FOR SEQUENCE-DIRECTED MOLECULAR BREEDING - The present invention provides breeding methods and compositions to enhance the germplasm of a plant by the use of direct nucleic acid sequence information. The methods describe the identification and accumulation of preferred nucleic acid sequences in the germplasm of a breeding population of plants. | 05-28-2009 |
| 20090136939 | Methods for diagnosis and therapy for pancreatic cancer and composition useful therein - Polynucleotide and polypeptide UKW are specific for pancreatic tumors. Diagnosis of UKW is therefore valuable for diagnosis of pancreatic tumors. Antibodies against UKW are, besides their value in diagnosis, useful as therapeutic agents for the treatment of pancreatic tumors. | 05-28-2009 |
| 20090136940 | PHOSPHORAMIDITE NUCLEOSIDE ANALOGS - Described are phosphoramidite nucleoside analog monomers, precursors thereof, and oligonucleotides including one or more of the monomers. The monomers can be used during automated synthesis of oligonucleotide derivatives, and allow for incorporation of one or several reporter groups, organic molecules, bio-molecules, small molecules or other chemical groups at the internucleoside phosphotriesters. Oligonucleotides including the monomers have a number of uses in therapeutic, diagnostic, and research applications. | 05-28-2009 |
| 20090136941 | METHODS, KITS AND COMPOSITIONS PERTAINING TO LINEAR BEACONS - This invention is directed to methods for determining amplified nucleic acid using Linear Beacons | 05-28-2009 |
| 20090136942 | Analysis of Extracellular RNA - The invention provides methods for detecting tumor-associated RNA in plasma, serum, and other bodily fluids. In particular, the invention provides methods for detecting translocated gene RNA, including fusion gene RNA, in plasma or serum or other bodily fluids. | 05-28-2009 |
| 20090136943 | METHOD OF DETECTING CANCER USING DELTA-CATENIN - The present invention provides a method for detecting or screening for the presence of cancer in a subject. The method comprises obtaining, providing or collecting a tissue or fluid sample (such as a urine sample) from said subject, and then determining the presence or absence of delta-catenin in said sample, or increased levels of delta-catenin in said sample as compared to a normal or control subject. The presence of delta-catenin in said sample, or increased levels of delta-catenin in said sample, indicating said subject is afflicted with or at least at risk of developing cancer. | 05-28-2009 |
| 20090136944 | Aberrantly Methylated Genes as Markers of Breast Malignancy - The invention is directed to a method of diagnosing a cell proliferative disorder of breast tissue by determining the methylation status of nucleic acids obtained from a subject. Aberrant methylation of several genes including TWIST, HOXA5, NES-1, retinoic acid receptor beta (RARβ), estrogen receptor (ER), cyclin D2, WT-1, 14.3.3 sigma, HIN-1, RASSF1A, and combinations of such genes serve as markers of breast malignancy. | 05-28-2009 |
| 20090136945 | COMPOSITIONS AND METHODS FOR ASSESSING DISORDERS - The present invention relates to compositions and methods for disorder (e.g., hyperproliferative disorders, inflammatory disorders) research, diagnosis, and treatment, including but not limited to, bio-markers specific for a particular disorder (e.g., cancer, systemic lupus erythematosus). In particular, the present invention relates to peripheral blood mononuclear cells (PBMCs) as bio-markers specific for particular disorders. | 05-28-2009 |
| 20090136946 | Automated Enumeration and Characterization of Circulating Melanoma Cells in Blood - The CellTracks® System provides a system to enumerate circulating melanoma cells in blood. The system immunomagnetically concentrates epithelial cells, fluorescently labels the cells and identifies and quantifies circulating melanoma cells. The absolute number of circulating melanoma cells detected in the peripheral blood tumor load is, in part, a factor in prediction of survival, time to progression, and response to therapy. Diagnosis and monitoring of melanoma has been limited by the inability to monitor circulating melanoma cells. The present invention provides a method to enumerate circulating melanoma cells in blood samples. Accordingly, this technology provides a means and device for monitoring disease progression in patients with melanoma. | 05-28-2009 |
| 20090136947 | METHOD FOR CONDUCTING AN ASSAY FOR NEUTRALIZING ANTIBODIES - A method for conducting a neutralization assay for the determination of the titer of antibodies in a sample is provided which is an improvement over existing neutralization assays because the sensitivity of the reporter gene assay to determine neutralization is increased by the use of a “one time use” cell line transformed with a reporter gene construct. The titer is determined for antibodies specific for a predetermined target molecule that activates the signal transduction activity of a cell surface protein or a pattern recognition receptor or are specific for an antagonist for the predetermined target molecule. | 05-28-2009 |
| 20090136948 | NANOCONFINEMENT- BASED DEVICES AND METHODS OF USE THEREOF - The present invention provides a device/kit and methods of use thereof in rapid detection of target molecule binding to a cognate binding partner. The methods, inter-alia, make use of a device comprising channels or reservoirs, which are linked to nanochannels, whereby upon application of the cognate binding partner to the nanochannel comprising the target molecule under flow, a detectable change in conductance, capacitance or fluorescence or surface potential occurs. | 05-28-2009 |
| 20090136949 | PROCESS FOR PREDICTING THE PROGNOSIS OF SQUAMOUS CELL LUNG CANCER - Disclosed in this specification is a method for predicting the prognosis of squamous cell lung cancer by observing regulatory changes in select miRNA sequences. These sequences may include hsa-mir-146b, hsa-mir-191, hsa-mir-206, hsa-mir-299-3p, hsa-mir-155, hsa-mir-15a, hsa-mir-122a, hsa-mir-513, hsa-mir-184, hsa-mir-511, hsa-mir-100, hsa-mir-10a, hsa-mir-453, hsa-mir-379, hsa-mir-202, hsa-mir-21, hsa-mir-126, hsa-mir-494, hsa-mir-432, hsa-mir-370, and combinations of these sequences. | 05-28-2009 |
| 20090136950 | IMMUNOGLOBULIN DISPLAY VECTORS - Disclosed are mammalian expression vectors for expressing simultaneous expression of immunoglobulins as both a secreted and cell surface bound form. | 05-28-2009 |
| 20090136951 | QUANTIFICATION OF NUCLEIC ACID MOLECULES USING MULTIPLEX PCR - Described are novel quantification methods and systems that permit, within the context of multiplex PCR, the quantification of all targets within a single reaction tube. The methods employ quantitation algorithms applied to the amplification profiles of internal calibration controls or standards utilizing a plurality of nucleic acid templates that are amplified within the same reaction tube as the nucleic acid target(s) interrogated. | 05-28-2009 |
| 20090136952 | MANGANESE SUPEROXIDE DISMUTASE VAL16ALA POLYMORPHISM PREDICTS RESISTANCE TO CHEMOTHERAPEUTIC DRUG CANCER THERAPY - The present invention provides, for the first time, the finding that the manganese superoxide dismutase Val16Ala polymorphism is significantly associated with prognosis for cancer patients treated with chemotherapeutic drug therapy. The alanine allele is a novel biomarker that predicts poor response and poor outcome to chemotherapeutic drug cancer therapy. Conversely, the valine allele predicts a good response and a good outcome to chemotherapeutic drug cancer therapy. Therefore, a genotype assay can be used to determine which alleles a subject is carrying, and subsequently this information can be used to determine if chemotherapeutic drug therapy is appropriate, and to customize therapy according to the patient's MnSOD genotype. | 05-28-2009 |
| 20090136953 | MOLECULAR DIAGNOSTIC METHOD FOR DETERMINING THE RESISTANCE OF A MICROORGANISM TO AN ANTIBIOTIC | 05-28-2009 |
| 20090136954 | GENETIC MARKERS FOR SCD OR SCA THERAPY SELECTION - Variations in certain genomic sequences useful as genetic markers of Sudden Cardiac Death (“SCD”), or Sudden Cardiac Arrest (“SCA”) risk, are described. Novel genetic markers useful in assessing the risk of SCD, or SCA, and kits containing the same are provided herein. Methods of distinguishing patients having an increased susceptibility to SCD, or SCA, through use of these markers, alone or in combination with other markers, are also provided. Further, methods of assessing the need for an Implantable Cardio Defibrillators (“ICD”) in a patient are taught. | 05-28-2009 |
| 20090136955 | Protocols for making hepatocytes from embryonic stem cells - This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy. | 05-28-2009 |
| 20090136956 | Use of Dual-Tags for the Evaluation of Genomic Variable Repeat Regions - The methods and compositions of the present invention allow for the evaluation of a nucleotide expansion, contraction or deletion. In one embodiment, the present invention provides a method for detecting a nucleotide expansion, contraction or deletion comprising amplifying a target nucleic acid sequence with a primer pair, each primer comprising a target specific sequence and a differentiating tag sequence, labeling the target nucleic acid sequence to produce a dual-tagged amplification product; digesting the dual-tagged amplification product to produce an expansion region fragment and an internal control fragment; hybridizing the fragments to separate capture complexes; detecting the signals produced by the labels on the immobilized fragments and comparing the intensity of the signals to detect the nucleotide expansion, contraction or deletion region. | 05-28-2009 |
| 20090136957 | Methods and compositions for regulating cell cycle progression via the miR-106B family - In one aspect, a method is provided of inhibiting proliferation of a mammalian cell comprising introducing into said cell an effective amount of at least one microRNA-specific inhibitor of at least one miR-106b family member. In another aspect a method is provided for accelerating proliferation of a mammalian cell comprising introducing into said cell an effective amount of at least one miR-106b family member. | 05-28-2009 |
| 20090136958 | Capture, recapture, and trapping of molecules with a nanopore - In a molecular analysis system, there is provided a structure including a nanopore and first and second fluidic reservoirs. The two reservoirs are fluidically connected via the nanopore. A detector is connected to detect molecular species translocation of the nanopore, from one of the two fluidic reservoirs to the other of the two fluidic reservoirs. A controller is connected to generate a control signal to produce conditions at the nanopore to induce the molecular species to re-translocate the nanopore at least once after translocating the nanopore. This enables a method for molecular analysis in which a molecular species is translocated a plurality of times through a nanopore in a structure between two fluidic reservoirs separated by the structure. | 05-28-2009 |
| 20090136959 | Novel genes encoding proteins having prognostic, diagnostic preventive, therapeutic, and other uses - The invention provides isolated nucleic acids encoding a variety of proteins having diagnostic, preventive, therapeutic, and other uses. These nucleic and proteins are useful for diagnosis, prevention, and therapy of a number of human and other animal disorders. The invention also provides antisense nucleic acid molecules, expression vectors containing the nucleic acid molecules of the invention, host cells into which the expression vectors have been introduced, and non-human transgenic animals in which a nucleic acid molecule of the invention has been introduced or disrupted. The invention still further provides isolated polypeptides, fusion polypeptides, antigenic peptides and antibodies. Diagnostic, screening, and therapeutic methods using compositions of the invention are also provided. The nucleic acids and polypeptides of the present invention are useful as modulating agents in regulating a variety of cellular processes. | 05-28-2009 |
| 20090136960 | METHODS AND COMPOSITIONS FOR THE IDENTIFICATION OF CANCER MARKERS - The present invention relates to methods and compositions for the identification of cancer markers. In particular, the present invention provides methods and compositions for the identification of glycosylated proteins and protein glycosylation patterns. The present invention further provides cancer markers identified using the described methods. | 05-28-2009 |
| 20090136961 | Novel compositions and methods for identification, assessment, prevention, and therapy of ovarian cancer - The present invention is directed to the identification of markers that can be used to determine whether ovarian cancer is sensitive or resistant to a therapeutic agent. In particular, the present invention is directed to the use of certain combinations of markers, wherein the expression of the markers correlates with sensitivity or resistance to a therapeutic agent. Thus, by examining the expression of the individual markers of a marker set, also referred to as the expression profile of the marker set, it is possible to determine whether a therapeutic agent, or combination of agents, will be most likely to reduce the growth rate of the ovarian cancer. | 05-28-2009 |
| 20090136962 | DIAGNOSTIC MARKERS FOR CANCER - The present invention provides compositions and methods useful for diagnosing cancer and for monitoring the progression and treatment of cancer, as well as targets for treating cancer. The invention provides sperm and testis associated antigens, such as the protein CABYR, as biomarkers and as targets for chemotherapy. | 05-28-2009 |
| 20090136963 | METHODS OF CONCENTRATING AN ANALYTE - A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle. | 05-28-2009 |
| 20090142749 | Assessment of disease risk by quantitative determination of epimutation in normal tissues - An assay for assessing the risk of disease in an individual, wherein said assay comprises the steps of isolating a population of cells from normal tissue of said individual, and quantitatively determining the frequency of epimutation of a particular gene in said population of cells, wherein the epimutation of said gene is associated with said disease and said gene is other than one that is subject to normal parent of origin-specific expression. Preferably, the epimutation is DNA methylation of a tumour suppressor gene such as hMLH1, hMSH2, APC 1A, APC 1B and p16. | 06-04-2009 |
| 20090142750 | Method of detecting and identifying gram-negative obligative anaerobic bacterium - There is provided a method of detecting potentially beer-spoiling obligatory anaerobic gram-negative bacteria, and a method of simultaneously detecting and distinguishing different beer-spoilage microorganisms including such bacteria. | 06-04-2009 |
| 20090142751 | ANALYZING POLYNUCLEOTIDE SEQUENCES - An apparatus is provided for analysing a polynucleotide. The apparatus includes: a support having an impermeable surface; porous material attached to the impermeable surface; and an array of oligonucleotides with predetermined sequences attached to the porous material. The array includes at least two defined cells, the sequence of the oligonucleotides of a first cell being different from the sequence of the oligonucleotides of a second cell, and the oligonucleotides being shorter than the polynucleotide. | 06-04-2009 |
| 20090142752 | Snap-Back Primers And Detectable Hairpin Structures - The present invention provides methods, compositions, and kits comprising snap-back primers used for forming 3′ hairpin structures, 5′ hairpin structures, and double hairpin structures. The hairpin structures may be used for detecting target sequences (e.g., such as small RNA target sequence), for detecting polymorphisms in target sequences (e.g., such as polymorphisms located near the 5′ or 3′ ends of the target sequence), or other nucleic acid characterization methods. In certain embodiments, the hairpin structures form invasive cleavage structures (e.g., in combination with a probe or upstream oligonucleotide) which may be cleaved by structure-specific enzymes in order to detect the presence or absence of a particular nucleotide or nucleotide sequence. | 06-04-2009 |
| 20090142753 | Compositions and methods for detecting compounds to treat a neurological disorder - The present invention generally provides compositions and methods that can be used to detect compounds that modulate the activity of at least one of the DJ-1, Parkin and Pink-1 genes. | 06-04-2009 |
| 20090142754 | RNA Detection Assays - The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. | 06-04-2009 |
| 20090142755 | ASSAY FOR DETECTING GENETIC ABNORMALITIES IN GENOMIC NUCLEIC ACIDS - The present invention provides methods of detecting unamplifed genomic nucleic acid anchored to a solid support. The methods are useful for the detecting genetic abnormalities associated with various diseases, diagnosis, and prognosis. | 06-04-2009 |
| 20090142756 | PREDICTION OF BARE METAL STENT RESTENOSIS - Methods for predicting whether or not a patient is likely to experience restenosis after the placement of a bare metal stent are provided. The methods involve detecting and analyzing gene expression patterns of the cellular components of whole blood, where activation of selected genes has been found to be indicative of a high probability of restenosis. The method thus allows the identification, prior to placement of a stent, of patients who are i) likely to experience restenosis, and thus should receive a stent that includes anti-restenosis agents; or ii) unlikely to experience restenosis, and thus should receive a stent without anti-stenosis agents. | 06-04-2009 |
| 20090142757 | STRIP AND METHOD FOR DETECTING NUCLEOTIDE AMPLIFICATION PRODUCTS OF MYCOBACTERIUM TUBERCULOSIS AND NON-TUBERCULOUS MYCOBACTERIUM - The present invention provides a test strip and a method for rapid identifying the presence of the amplified DNA product of | 06-04-2009 |
| 20090142758 | STRATEGIES FOR SEQUENCING COMPLEX GENOMES USING HIGH THROUGHPUT SEQUENCING TECHNOLOGIES - A method for determining a genome sequence comprising the steps of digesting the genome with at least one first restriction endonuclease, ligating at least one adaptor to the restriction fragments of the first subset, selectively amplifying the first set of adaptor-ligated restriction fragments using a first primer combination wherein at least a first primer contains a first selected sequence at the 3′ end of the primer sequence, comprising 1-10 selective nucleotides, repeating these steps with at least a second primer combinations wherein the primer contains a different second selected sequence, fragmenting each of the subsets of amplified adaptor-ligated restriction fragments to generate sequencing libraries, determine the nucleotide sequence of the fragments, aligning the sequence of the fragments in each of the libraries to generate contigs, repeating these steps for one second and/or further restriction endonucleases, aligning the contigs obtained for each of the second and/or further restriction endonucleases to provide for a sequence of the genome. | 06-04-2009 |
| 20090142759 | qPCR array with IN SITU primer synthesis - Application of in situ oligonucleotide synthesis, using a maskless photolithographic oligonucleotide synthesis apparatus or by other means, for direct fabrication of polymerase chain reaction (PCR) primers in situ in PCR reaction wells. The synthesized oligonucleotides contain an enzymatically degradable linker sequence and a specific primer sequence. The method may be used for manufacturing of quantitative PCR (qPCR) arrays containing a plurality of independent qPCR assays while eliminating the need for presynthesized primer libraries. | 06-04-2009 |
| 20090142760 | Nucleic Acid Sequences Associated with Cell States - The present invention is directed to nucleic acid sequences whose expression is associated with different cell states, including nucleic acid sequences whose expression is induced at least 100-fold, or alternatively upregulated, in cells exhibiting asymmetric self-renewal relative to other cells. The invention is also directed to nucleic acid sequences whose expression is induced at least 100-fold, or alternatively upregulated, in cells exhibiting symmetric self-renewal relative to other cells. | 06-04-2009 |
| 20090142761 | METHODS AND KITS FOR METHYLATION DETECTION - Ligation-based methods and kits are disclosed for determining the degree of methylation of one or more target nucleotides. In certain embodiments, the methylation status of one or more target nucleotides is determined by generating misligation products. In certain embodiments, at least one target nucleotide is amplified prior to the ligation reaction. In certain embodiments, at least one ligation product, at least one ligation product surrogate, at least one misligation product, at least one misligation product surrogate, or combinations thereof are amplified. In certain embodiments, one or more ligation probes comprise at least one nucleotide analog, at least one Modification, at least one mismatched nucleotide, or combinations thereof. | 06-04-2009 |
| 20090142762 | Method of Screening for Binding Interaction Using Sets of Microparticles and Unique Probes - The present invention relates to methods for screening for binding interactions using multiple sets of microparticles, wherein said set has the same identifiable characteristic and wherein one of more sets comprise subsets of microparticles and said subset presents at least one unique probe that acts as a binding partner for a target molecule in a biological sample. In particular, the invention provides for methods of detecting tissue-typing antigens in donor tissue or recipient tissue using these multiple sets of microparticles. | 06-04-2009 |
| 20090142763 | NESTED PCR-BASED METHOD FOR SPECIFIC GENOTYPING OF THE FC GAMMA RECEPTOR IIIA GENE - The application describes a novel method for detection of a single nucleotide polymorphism, e.g., the 158 F/V polymorphism, in the FcγRIIIa gene using nested PCR to amplify large gene amplicons to aid in, e.g., genotyping applications. Thus, based on the results obtained in FcγRIIIa genotyping analysis, the present invention provides specific, efficient, reproducible, and accurate detection of such polymorphisms in genomic DNA. | 06-04-2009 |
| 20090142764 | Methods and Kits for Multiplex Amplification of Short Tandem Repeat Loci - Methods and materials are disclosed for use in simultaneously amplifying at least 11 specific STR loci of genomic DNA in a single multiplex reaction, as are methods and materials for use in the analysis of the products of such reactions. Included in the present invention are materials and methods for the simultaneous amplification of 16 specific loci in a single multiplex reaction, comprising the 10 AmpFISTR® SGMplus® STR loci, the Amelogenin locus, and 5 new STR loci, including methods and materials for the analysis of these loci. | 06-04-2009 |
| 20090142765 | METHOD AND APPARATUS FOR RAPIDLY COUNTING AND IDENTIFYING BIOLOGICAL PARTICLES IN A FLOW STREAM - A method for increasing the throughput, or the precision, or both the precision and the throughput, of a flow cytometer, or of a hematology analyzer employing a flow cytometer, and for further reducing the complexity of such a cytometer or analyzer, by utilizing the technique of laser rastering in combination with a lysis-free single-dilution method. Laser rastering involves sweeping a laser beam across a flowing sample stream in a hematology analyzer. A lysis-free single-dilution method involves performing all the flow cytometer measurements on a sample using a single aliquot, a single lysis-free reagent solution, a single dilution, and a single pass of said dilution through the measurement apparatus. An apparatus suitable for carrying out the method of this invention comprises an optical module comprising a source of light, a scanning device, a lens or system of lenses, a flowcell, detectors, and filters; and an electronic module comprising preamplifiers, analog signal conditioning elements, analog-to-digital converters, field-programmable gate arrays, digital signal processing elements, and data storage elements. | 06-04-2009 |
| 20090142766 | METHODS FOR MEASURING THE METABOLISM OF CNS DERIVED BIOMOLECULES IN VIVO - The present invention provides methods for measuring the metabolism of a central nervous system derived biomolecule implicated in a neurological and neurodegenerative disease or disorder. In particular, the method comprises measuring the in vivo metabolism of the biomolecule in the central nervous system of a subject. Also provided is a method for determining whether a therapeutic agent affects the in vivo metabolism of a central nervous system derived biomolecule. | 06-04-2009 |
| 20090142767 | Method for nucleic acid quantitation - It is intended to provide a novel convenient approach for DNA quantitative analysis that overcomes the disadvantages of conventional formulations. A standard DNA sample is prepared by introducing a single-base substitution into target DNA, and a predetermined amount thereof is mixed with a target DNA sample. The target and standard DNAs are amplified using the same primers designed to amplify a region comprising the single-base substitution site. To a hybridization product of a probe capable of binding to a site immediately before the single-base substitution site, ddATP, ddGTP, ddCTP, and ddTTP are sequentially added one by one to perform a complementary strand synthesis reaction. Luciferase reaction-induced luminescence derived from the formed pyrophosphoric acid is detected. The target DNA is quantitated from the amount of the detected luminescence and the amount of the added standard DNA sample. | 06-04-2009 |
| 20090142768 | PERFORIN-2 PROTEINS - Perforin-2 (P2) molecule is a pore forming protein. The 5′ untranslated region of the perforin-2 protein controls translational activity. Compositions include the perforin protein and the 5′ untranslated region. Methods of use include high-throughput screening assays for identification of therapeutic compounds in treatment of diseases. | 06-04-2009 |
| 20090142769 | Methods for determining anti-TNF therapeutic response - The present invention relates to methods for identifying patients that will respond to treatment with anti-TNF-therapy, i.e., anti-TNF responder patients. In particular, the present invention relates to determining response to an inhibitor of tumor necrosis factor (TNF) in a patient with a chronic inflammatory disease by determining the activity of type I interferon in the patient. The invention further relates to quantification of type I interferon as a measure of predicting responsiveness to anti-TNF therapy in patients with a chronic inflammatory disease such as rheumatoid arthritis (RA), psoriatic arthritis, ankylosing spondylitis, juvenile chronic arthritis, lupus, Crohn's disease, as well as for cardiovascular disease. | 06-04-2009 |
| 20090142770 | Hair Follicle Pharmacodynamic Assay for Telomerase Activity - The invention is directed to methods for determining the efficacy of treatment with telomerase modulators in mammals by the analysis of the level of telomerase reverse transcriptase activity in mammalian hair follicle cells. | 06-04-2009 |
| 20090142771 | Methods and Instruments for Processing a Sample in a Multi-Chambered Receptacle - A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle. | 06-04-2009 |
| 20090148833 | DEVICES FOR GENERATING DETECTABLE POLYMERS - This document provides systems, devices, and methods involved in generating detectable polymers. For example, diagnostic systems, diagnostic devices, primer systems, and collections of primer systems are provided. | 06-11-2009 |
| 20090148834 | Methods of use of alpha-methylacyl-CoA racemase in hormone refractory and metastatic prostate cancers - Methods for identifying patients having or at risk of developing prostate cancer (including hormone refractory or androgen independent prostate cancer) and patients having or at risk of developing a cancer arising from metastasis if a prostate cancer to another tissue, e.g., liver and lymph node, by measuring the expression or activity of alpha-methylacyl-CoA racemase are described. The invention also provides: methods of screening for compounds that can be used to treat prostate cancer (including hormone refractory or androgen independent prostate cancer) or metastases of prostate cancer by screening for compounds that modulate the expression or activity of the alpha-methylacyl-CoA racemase polypeptides or nucleic acids; a process for modulating (i.e., reducing) alpha-methylacyl-CoA racemase polypeptide or nucleic acid expression or activity, e.g., using the screened compounds; and methods for selecting patients for therapy with a compound that reduces the activity or expression of alpha-methylacyl-CoA racemase as well as methods for determining whether such a therapy should be continued in a patient. | 06-11-2009 |
| 20090148835 | METHOD FOR IDENTIFYING THE ORIGIN OF A COMPOUND BIOLOGICAL PRODUCT - The present invention relates to an identification method. In particular, a method for identifying the origin of a compound biological product, including the batch of origin, but also in some cases the actual biological sources of a compound biological product. | 06-11-2009 |
| 20090148836 | Method for Rapid Detection and Identification of Bioagents - Method for detecting and identifying unknown bioagents, including bacteria, viruses and the like, by a combination of nucleic acid amplification and molecular weight determination using primers which hybridize to conserved sequence regions of nucleic acids derived from a bioagent and which bracket variable sequence regions that uniquely identify the bioagent. The result is a “base composition signature” (BCS) which is then matched against a database of base composition signatures, by which the bioagent is identified. | 06-11-2009 |
| 20090148837 | Method for Rapid Detection and Identification of Bioagents - Method for detecting and identifying unknown bioagents, including bacteria, viruses and the like, by a combination of nucleic acid amplification and molecular weight determination using primers which hybridize to conserved sequence regions of nucleic acids derived from a bioagent and which bracket variable sequence regions that uniquely identify the bioagent. The result is a “base composition signature” (BCS) which is then matched against a database of base composition signatures, by which the bioagent is identified. | 06-11-2009 |
| 20090148838 | Methods for analysis of gene expression - This invention provides methods, compositions and kits for gene expression analysis and gene expression profiling. The methods of the invention are highly sensitive; have a wide dynamic range; are rapid and inexpensive; have a high throughput; and allow the simultaneous differential analysis of a defined set of genes. The methods, compositions and kits of the invention also provide tools for gene expression data collection and relational data analysis. | 06-11-2009 |
| 20090148839 | METHOD FOR EVALUATING CELL POPULATIONS - The invention describes specific sialylated structures present on human stem cells and cell populations derived thereof. The invention is especially directed to methods to control the status of stem cells by observing changes in sialylation of the cells; and control of potential contaminations of biological materials; and reagents and methods used in connection with the cells in order to avoid alterations of the cell glycosylation by contaminating materials. The invention is further directed to novel stem cells, the glycosylation of which has been specifically altered. | 06-11-2009 |
| 20090148840 | Method for detecting colon cancer markers - A method of detecting a tumor marker for the diagnosis of large bowel cancer and adenomatous polyposis coli comprising the steps of a) collecting and freezing feces, b) homogenizing the frozen feces in the presence of an RNAase inhibitor and preparing a suspension, c) extracting RNA from the obtained sample from which RNA is extracted, d) obtaining cDNA by reverse transcribing the extracted RNA, e) amplifying the obtained cDNA, and f) detecting the amplified cDNA, characterized in that the tumor marker is one or more tumor markers selected from the group consisting of COX-2, SNAIL and MMP-7 (with the proviso that a case where only COX-2 or MMP-7 is selected is excluded). | 06-11-2009 |
| 20090148841 | MULTIPLEXED GENOMIC GAIN AND LOSS ASSAYS - Encoded bead multiplex assays for chromosomal gains and losses are provided that provide the benefits of complex, large template DNA sources, such as BAC DNA, as the probe material without bead networking or other assay performance problems. Reagents for assaying DNA are described herein which include a plurality of encoded particles having attached amplicons amplified from a template DNA sequence. Each individual attached amplicon includes a nucleic acid sequence identical to a random portion of the template DNA sequence, wherein the amplicons together represent substantially the entire template DNA and wherein the nucleic acid sequence identical to a random portion of the template DNA sequence of each individual amplicon is shorter than the entire template DNA. | 06-11-2009 |
| 20090148842 | Preparation of templates for methylation analysis - The invention relates to a method of preparing and using a library of template polynucleotides suitable for use as templates in solid-phase nucleic acid amplification and sequencing reactions to determine the methylation status of the cytosine bases in the library. In particular, the invention relates to a method of preparing and analysing a library of template polynucleotides suitable for methylation analysis. | 06-11-2009 |
| 20090148843 | Means and Methods for the Prediction of Joint Destruction - The present invention relates to a method of diagnosing and/or predicting joint destruction, early joint destruction and/or accelerated joint destruction in particular, in rheumatoid arthritis, comprising determining in a sample obtained from an individual the presence of at least one nucleic acid sequence encoding an IL-4 receptor (IL-4R) which contains a mutation in position 465 of the nucleotide sequence of the wild-type IL4R as shown in SEQ ID NO: 1, whereby at said position the nucleotide A is replaced or a nucleic acid sequence encoding an IL-4 receptor (IL-4R), said IL-4R comprising at position 75 as shown in SEQ ID NO: 2 a valine instead of an isoleucine. Furthermore, the invention provides for a method of diagnosing and/or predicting early joint destruction and/or accelerated joint destruction comprising determining in a sample obtained from an individual the presence of an encoded IL-4 receptor (IL-4R) which comprises at the homologous position 75 of IL-4 receptor as depicted in SEQ ID NO: 2 a mutation, said mutation comprising the exchange from an isoleucine to a valine. In addition, the present invention relates to a use of specific probes and/or primers for the preparation of a diagnostic composition for diagnosing and/or predicting early joint destruction and/or accelerated joint destruction in particular in rheumatoid arthritis. | 06-11-2009 |
| 20090148844 | DNA MARKER FOR MEAT TENDERNESS IN CATTLE - A method for assessing the tenderness of meat from an animal, comprising the step of testing the animal for a genetic marker in the calpain3 (CAPN3) gene associated with Warner-Bratzler peak force variation or for a genetic marker located other than in CAPN3 which shows allelic association therewith. | 06-11-2009 |
| 20090148845 | ENZYME MEASUREMENT ASSAY USING A MODIFIED SUBSTRATE COMPRISING A SUBSTRATE ATTACHED TO A MACROMOLECULE VIA A SPACER - The invention provides novel reagents and methodologies for detecting free versus bound compounds. It is particularly useful to detect thrombin when it is not bound to A2M in the presence of thrombin bound to A2M by using a modified substrate that is sterically hindered from reacting with the bound thrombin. | 06-11-2009 |
| 20090148846 | Modified Oligonucleotides and Applications Thereof - Disclosed, among other things, are primers containing certain modified nucleobases in the 3′ terminal region of the primers that provide reduced formation of primer-dimers during amplification reactions, and various methods of use thereof. | 06-11-2009 |
| 20090148847 | RAPID MAGNETIC FLOW ASSAYS - Disclosed is an improvement in methods for nucleic acid and immunological bioassays. The methods comprise a step for “sweeping” paramagnetic bead: target molecule complexes so as to capture them with an affinity capture agent on a test pad by moving a magnetic force field from outside to inside the test pad area so as to bring into contact the paramagnetic complexes with the capture agent, while sweeping any unbound paramagnetic material off the test pad by moving the magnetic field from inside to outside the test pad area. Surprisingly, the paramagnetic complexes are rapidly affinity-extracted from the moving magnetic field. | 06-11-2009 |
| 20090148848 | PITX2 Polynucleotide, Polypeptide and Methods of Use Therefor - A novel T-type calcium channel (CACNA1G) is provided, as are polynucleotides encoding the same. CACNA1G has been implicated in cellular proliferative disorders. More specifically, it has been observed that the methylation state of specific regions within CpG islands associated with the CACNA1G gene correlates with a number of cancerous phenotypes involving a variety of tissue and cell types. Also provided are methods for detecting cellular proliferative disorders by determining the methylation state of genes or regulatory regions associated therewith, including CACNA | 06-11-2009 |
| 20090148849 | ONE-STEP TARGET DETECTION ASSAY - The present invention provides nucleic acid amplification, detection, and genotyping techniques. In one embodiment, the present invention provides a method for amplifying and detecting a target nucleic acid sequence by providing a first primer pair comprising: a first primer comprising a target specific sequence, a tag sequence 5′ of the target specific sequence, and a blocker between the target specific sequence and the tag sequence, and a second primer comprising a target specific sequence; providing a reporter attached to either the second primer or to a dNTP; providing a capture complex comprising an anti-tag sequence attached to a solid support; combining the first primer pair, the capture complex, the reporter, and a sample comprising a target nucleic acid sequence under conditions suitable for amplification of the target nucleic acid sequence and hybridization of the amplified target nucleic acid sequence to the capture complex; and detecting the amplified target nucleic acid sequence. | 06-11-2009 |
| 20090148850 | Methods for identifying modulators of P2RY14 - Methods for identifying modulators of P2RY14 are described. The methods are particularly useful for identifying agents that are useful for treating metabolic syndrome. | 06-11-2009 |
| 20090148851 | Methods, kits, and compositions for detecting enzyme activity - The inventive subject matter relates to methods, kits, and compositions for detecting enzyme activity in a biological sample. In particular, the inventive subject matter relates to methods, kits, and compositions for detecting von Willebrand factor degrading enzyme activity in a biological sample. | 06-11-2009 |
| 20090148852 | Sensitive method for detecting low levels of ATP - Methods are provided for sensitive detection of adenosine triphosphate (ATP) in samples using the luciferin-luciferase reaction. Aspects include using a pH composition that maximizes a signal to noise ratio. The maximum signal to noise ratio can be particularly useful with recombinant luciferase including recombinant Coleoptera luciferase. | 06-11-2009 |
| 20090148853 | BIOMARKERS FOR PREDICTING THE SENSITIVITY OF CELLS TO IMMUNOMODULATORY COMPOUNDS DURING TREATMENT OF NON-HODGKIN'S LYMPHOMA - Provided herein are the biomarkers for monitoring the treatment by immunomodulatory compounds. The use of biomarkers such as SPARC, p21, and cyclin D1 mRNA or protein levels as biomarkers to predict whether an immunomodulatory compound is likely to be successful in treating certain types of cancer, such as NHL, is also provided. Further, the expression of these genes or proteins can be used to monitor progress of treatment effectiveness and patient compliance in cancer patients that are receiving treatment with immunomodulatory compounds. | 06-11-2009 |
| 20090148854 | NUCLEIC ACID AND CORRESPONDING PROTEIN ENTITLED 184P1E2 USEFUL IN TREATMENT AND DETECTION OF CANCER - A novel gene (designated 184P1E2) and its encoded protein, and variants thereof, are described wherein 184P1E2 exhibits tissue specific expression in normal adult tissue, and is aberrantly expressed in the cancers listed in Table I. Consequently, 184P1E2 provides a diagnostic, prognostic, prophylactic and/or therapeutic target for cancer. The 184P1E2 gene or fragment thereof, or its encoded protein, or variants thereof, or a fragment thereof, can be used to elicit a humoral or cellular immune response; antibodies or T cells reactive with 184P1E2 can be used in active or passive immunization. | 06-11-2009 |
| 20090148855 | NUCLEIC ACID ENCODING OR TARGETING SODIUM CHANNEL SCN3A ALPHA SUBUNITS - The present invention relates to epilepsy. More particularly, the present invention relates to idiopathic generalized epilepsy (IGE) and to the identification of three genes mapping to chromosome 2, which show mutations in patients with epilepsy. The invention further relates to nucleic acid sequences, and protein sequences of these loci (SCNA) and to the use thereof to assess, diagnose, prognose or treat epilepsy, to predict an epileptic individual's response to medication and to identify agents which modulate the function of the SCNA. The invention also provides screening assays using SCN1A, SCN2A and/or SCN3A which can identify compounds which have therapeutic benefit for epilepsy and related neurological disorders. | 06-11-2009 |
| 20090155775 | CLONED DNA POLYMERASES FROM THERMOTOGA AND MUTANTS THEREOF - The invention relates to a substantially pure thermostable DNA polymerase from | 06-18-2009 |
| 20090155776 | Fetal methylation markers - This application describes the discovery that, in a pregnant woman, certain genes (such as RASSF1A, APC, CASP8, RARB, SCGB3A1, DAB2IP, PTPN6, THY1, TMEFF2, and PYCARD) originated from a fetus are highly methylated, whereas the same genes of maternal origin are unmethylated. This discovery allows the easy detection of one or more of these methylated fetal genes in a biological sample from a pregnant woman, serving as a universal indicator of the presence of fetal DNA in the sample. These fetal methylation markers are particularly useful as positive controls for a non-invasive analytical process during which the quality and quantity of fetal DNA are monitored. These newly identified fetal markers can also be measured directly for diagnosis of certain pregnancy-related conditions. | 06-18-2009 |
| 20090155777 | Methods for performing direct enzymatic reactions involving nucleic acid molecules - Methods for performing a direct enzymatic reaction involving a nucleic acid molecule include performing the enzymatic reaction directly using a biological specimen in a reaction mixture containing a zwitterionic buffer and/or a non-reducing carbohydrate to prevent the biological specimen from inhibiting the enzymatic reaction, in which a nucleic acid molecule present in the biological specimen is not purified before the enzymatic reaction, and obtaining a product from the enzymatic reaction. | 06-18-2009 |
| 20090155778 | Mental Disorder-Related Gene and Use of the Same - Disclosed is useful means for the therapy or diagnosis of a mental disorder. A method for screening for a compound which is effective for a mental disorder, comprising the steps of (1) providing a cell capable of expressing a gene (target gene) selected from the group consisting of a gene having the nucleotide sequence depicted in SEQ ID NO:1, a gene having the nucleotide sequence depicted in SEQ ID NO:2, a gene having the nucleotide sequence depicted in SEQ ID NO:3 and genes homologous to these genes; (2) exposing the cell to a compound to be tested; (3) determining the amount of a product of the target gene expressed in the cell after the exposure to the compound; and (4) determining the change in amount of the product of the target gene under the influence of exposure to the compound. | 06-18-2009 |
| 20090155779 | APTAMERS THAT BIND TO PRION PROTEIN - Compositions are provided in the form of nucleotide aptamerss that are capapble of binding PrP, and in some embodiments, differentially binding PrP isoforms. Also provided are methods for identifying PrP in a sample, and in some embodiments, either selectively removing PrP or PrP isoforms from a sample, or inactivating them within a sample. | 06-18-2009 |
| 20090155780 | Methods for determining genetic haplotypes and DNA mapping - Improved methods of genetic haplotyping and DNA sequencing and mapping, including methods for making amplified ssDNA, methods for allele determination, and a DNA barcoding strategy based on direct imaging of individual DNA molecules and localization of multiple sequence motifs or polymorphic sites on a single DNA molecule. | 06-18-2009 |
| 20090155781 | High throughput genome sequencing on DNA arrays - The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences using adaptors interspersed in target polynucleotides. The sequence information can be new, e.g. sequencing unknown nucleic acids, re-sequencing, or genotyping. The invention preferably includes methods for inserting a plurality of adaptors at spaced locations within a target polynucleotide or a fragment of a polynucleotide. Such adaptors may serve as platforms for interrogating adjacent sequences using various sequencing chemistries, such as those that identify nucleotides by primer extension, probe ligation, and the like. Encompassed in the invention are methods and compositions for the insertion of known adaptor sequences into target sequences, such that there is an interruption of contiguous target sequence with the adaptors. By sequencing both “upstream” and “downstream” of the adaptors, identification of entire target sequences may be accomplished. | 06-18-2009 |
| 20090155782 | Homoeologous Region Determining Method by Homo Junction Fingerprint Method, Homoeologous Region Determining Device, and Gene Screening Method - To provide a method for efficiently searching for a recessive disease gene without needing any pedigree analysis. In a homoeologous region determining method, the following steps are conducted. It is determined whether or not the base constituting a polymorphic marker of a sample DNA of diploid or higher polyploidy is a homojunction. Homojunction region information representing the region of the sample DNA where polymorphic markers determined as continuous homojunctions acquired. If the continuous probability and/or continuous distance of the polymorphic markers contained in the homojunction region information satisfy a predetermined determination condition, the homojunction region is determined as a homoeologous region. A homoeologous region determining device and a gene screening method for identifying a disease susceptibility gene from the determined homoeologous region are also provided. | 06-18-2009 |
| 20090155783 | Method of preparing a biological specimen slide - A method of preparing a slide of a biological specimen, including the steps of (a) providing a slide containing a biological specimen and a cover slip, (b) placing a liquid non-evaporating sealing compound such as mineral oil at spaced locations around an area on the slide and (c) placing the cover slip over the specimen area whereby the specimen and reagent is between the slide and the cover slip and the sealing compound spreads to define a closed boundary around the specimen in the space between the slide and the cover slip. Testing of the biological specimen may then be performed automatically. | 06-18-2009 |
| 20090155784 | ASSESSMENT OF ASTHMA AND ALLERGEN-DEPENDENT GENE EXPRESSION - The present invention provides methods for the assessment, diagnosis, or prognosis of asthma including methods for providing an assessment, diagnosis, or prognosis comprising the step of exposing a sample derived from a patient to an allergen in vitro. The present invention also provides methods for selecting, as well as evaluating the effectiveness of, asthma treatments. The markers of the present invention can be used in methods to identify or evaluate agents capable of modulating marker expression levels in subjects with asthma | 06-18-2009 |
| 20090155785 | REAL-TIME COLORIMETRIC SCREENING INHIBITORS OF ENDONUCLEASE WITH GOLD NANOPARTICLE SUBSTRATE - The invention provides methods for screening a compound for its effect on endonuclease activity. The methods comprise providing a compound to be screened utilizing a gold nanoparticle aggregate as the substrate for the endonuclease. The gold nanoparticle aggregate is formed by the hybridization of oligonucleotides attached to the nanoparticles, with or without the presence of a third linker oligonucleotide. The hybridized oligonucleotide duplex serves as a substrate for the endonuclease. A detectable change is brought about in the presence of the endonuclease activity. A decrease in the detectable change reflects the reduced levels of endonuclease activities as a result of the effects of endonuclease inhibitors. The present invention also provides kits for screening an endonuclease inhibitor. | 06-18-2009 |
| 20090155786 | COMPOSITIONS AND METHODS FOR DETECTING MARKERS OF CANCER - Disclosed herein are compositions and methods for detecting one or more markers indicative of cancer. In one instance the marker is one or more methylated genes, such as SFRPs. In another instance the marker is an altered protein, such as p53. | 06-18-2009 |
| 20090155787 | Mutations in the BCR-ABL tyrosine kinase associated with resistance to STI-571 - The invention described herein relates to novel genes and their encoded proteins, termed Mutants Associated with Resistance to STI-571 (e.g., T315I Bcr-Abl), and to diagnostic and therapeutic methods and compositions useful in the management of various cancers that express MARS. The invention further provides methods for identifying molecules that bind to and/or modulate the functional activity of MARS. | 06-18-2009 |
| 20090155788 | GENE EXPRESSION MARKERS FOR INFLAMMATORY BOWEL DISEASE - The present invention provides for a method of detecting the presence of inflammatory bowel disease in gastrointestinal tissues or cells of a mammal by detecting decreased expression of Indian Hedgehog (Ihh) and/or increased expression of Defensin A5 (DefA5) and/or Defensin A6 (DefA6) in the tissues or cells of the mammal relative to a control. | 06-18-2009 |
| 20090155789 | GENES FROM THE 20Q13 AMPLICON AND THEIR USES - The present invention relates to cDNA sequences from a region of amplification on chromosome 20 associated with disease. The sequences can be used in hybridization methods for the identification of chromosomal abnormalities associated with various diseases. The sequences can also be used for treatment of diseases. | 06-18-2009 |
| 20090155790 | CRAB-PII DIRECTED DIAGNOSTICS FOR NEOPLASTIC DISEASE - Disclosed are methods for diagnosing cancer in a test cell sample or fluid sample by detecting an increase in the level of expression of CRAB-PII in the test cell sample or fluid sample as compared to the level of expression of CRAB-PII in a control cell sample or fluid sample isolated from a normal subject. | 06-18-2009 |
| 20090155791 | Method for detecting methylation status by using methylation-independent primers - A reliable and highly sensitive method is provided for detecting methylation status of CpG-containing nucleic acids by nucleic acid amplification and melting curve analysis of amplification products. The methods and compositions employs a novel design of primers. CpG-containing methylation-independent oligonucleotide primers, wherein both unmethylated and methylated alleles of a CpG-containing nucleic acid can be detected by use of only one set of primers after the CpG-containing nucleic acid has been subjected to cytosine to thymine conversion of unmethylated Cytosine. The method is useful for detection of methylation status in for example cancer genes and other disease related genes, wherein methylation influences gene expression. | 06-18-2009 |
| 20090155792 | PREDICTORS OF LONG-TERM MORTALITY FOLLOWING CORONARY ARTERY BYPASS GRAFT SURGERY - The present invention relates, in general, to perioperative depression and, in particular, to methods of identifying individuals at risk of perioperative depression. | 06-18-2009 |
| 20090155793 | APPARATUS FOR HIGH THROUGHPUT SEQUENCING OF NUCLEIC ACIDS - A scalable reaction and detection system for automated high throughput sequencing of nucleic acids involving a combination of chemical processes and observation processes independent of the chemistry processes. Discrete functional units may be configured in a manner that allows the system to interchangeably utilize different sequencing reaction components in conjunction with discrete apparatus components for optical image collection and/or analysis. | 06-18-2009 |
| 20090155794 | CLONING MULTIPLE CONTROL SEQUENCES INTO CHROMOSOMES OR INTO ARTIFICIAL CENTROMERES - Artificially synthesizing 29 homozygous cystic fibrosis core panel controls demonstrates placing multiple homozygous mutant sequences on the same single control DNA sequence to streamline quality control by minimizing extra control assays, time, and costly formatted test materials and testing all controls during every test. Any rare or unavailable reported DNA sequence can be PCR amplified using primer pairs synthesized with the designated mutation or variant sequence with paired adjacent upstream and downstream primers to amplify target sequences in total genomic DNA. | 06-18-2009 |
| 20090155795 | BASELESS NUCLEOTIDE ANALOGUES AND USES THEREOF - A method of detecting a target nucleic acid. | 06-18-2009 |
| 20090155796 | MARKER FOR CANCER PROGNOSIS AND METHODS RELATED THERETO - The present invention is related to the novel discovery that HIF- | 06-18-2009 |
| 20090155797 | Methods For Generating Enhanced Antibody-Producing Cell Lines With Improved Growth Characteristics - The use of mismatch repair (MMR) defective antibody producer cells offers a method to generate subclone variants with elevated protein production such as antibodies. Using MMR defective cells and animals, new cell lines and animal varieties with novel and useful properties such as enhanced protein production can be generated more efficiently than by relying on the natural rate of mutation. These methods are useful for generating genetic diversity within host cells to alter endogenous genes that can yield increased titer levels of protein production. By employing this method, two genes were discovered whose suppressed expression is associated with enhanced antibody production. Suppressed expression of these genes by a variety of methods leads to increased antibody production for manufacturing as well as strategies for modulating antibody production in immunological disorders. Moreover, the suppression of these two genes in host cells can be useful for generating universal high titer protein production lines. | 06-18-2009 |
| 20090155798 | TLE3 AS A MARKER FOR CHEMOTHERAPY - Methods of using TLE3 as a marker for predicting the likelihood that a patient's cancer will respond to chemotherapy. Methods of using TLE3 as a marker for selecting a chemotherapy for a cancer. | 06-18-2009 |
| 20090155799 | METHODS FOR DIAGNOSING PANCREATIC CANCER USING REG4 PROTEIN - REG4, a new member of REG family was identified as a biomarker of pancreatic adenocarcinoma. The present invention provides sandwich ELISA to detect serum REG4 in patients with resectable pancreatic cancers i.e. PDACs. The present invention also provides a method for diagnosing a pancreatic cancer using REG4 as a serological marker. | 06-18-2009 |
| 20090155800 | BIOSENSOR HAVING NANO WIRE AND MANUFACTURING METHOD THEREOF - There is provided a biosensor capable of increasing a detecting sensitivity of a target substance by using a nano wire having excellent electrical characteristics and by immobilizing a receptor of the target substance to be detected on a substrate which is disposed between a nano wire and another nano wire and a method for manufacturing the same. A biosensor according to the present invention can be manufactured with an arrangement in which the nano wire is selectively arranged on a solid substrate in a matrix and, therefore, many materials can be detected at the same time. Particularly, since the degradation of electrical characteristics of the nano wire can be prevented in the present invention, a target substance is very sensitively detected through a small amount thereof. | 06-18-2009 |
| 20090155801 | METHOD OF SEQUENCING DNA - The present invention provides a method of identifying a base at a target position in a sample nucleic acid sequence, said method comprising: subjecting a primer hybridised to said sample nucleic acid immediately adjacent to the target position, to a polymerase primer extension reaction in the presence of a nucleotide, whereby the nucleotide will only become incorporated if it is complementary to the base in the target position, and determining whether or not said nucleotide is incorporated by detecting whether PPi is released, the identity of the target base being determined from the identity of any nucleotide incorporated, wherein, where said nucleotide comprises an adenine base, an α-thio triphosphate analogue of said nucleotide is used, ant the Rp isomer of said analogue and/or the degradation products of said analogue are eliminated from the polymerase reaction step. | 06-18-2009 |
| 20090155802 | Mutant DNA Polymerases with Improved Pyrophasphorolysis Activated Polymerization (PAP) Ability - Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. | 06-18-2009 |
| 20090155803 | METHODS FOR TISSUE ANALYSIS - This invention relates to methods of analyzing a tissue sample from a subject. In particular the invention combines morphological staining and/or immunohistochemistry (IHC) with fluorescence in situ hybridization (FISH) within the same section of a tissue sample. The analysis can be automated or manual. The invention also relates to kits for use in the above methods. | 06-18-2009 |
| 20090155804 | DISEASE PATHWAY-BASED METHOD TO GENERATE BIOMARKER PANELS TAILORED TO SPECIFIC THERAPEUTICS FOR INDIVIDUALIZED TREATMENTS - The increased efficacy and reduced unwanted side effects of drugs can be insured by treating only responsive patients. In an embodiment of the invention, signaling pathways that a particular drug interferes with, are derive together with predictive biomarkers and dynamic biomarker that can read the activity of these pathways before and after drug treatment in order to select a responder patient population. In an alternative embodiment of the invention, certain core pathways that the drug does not interfere with and that are known to be causally involved in a particular disease(s) can be identified, and derive the biomarkers for those to be able to exclude these patients that suffer from a disease in which those drug non effected pathways are involved from being treated with the specific drug in question. | 06-18-2009 |
| 20090155805 | COPY NUMBER ALTERATIONS THAT PREDICT METASTATIC CAPABILITY OF HUMAN BREAST CANCER - Disclosed in this specification is a method of defining chromosome regions of prognostic value by summarizing the significance of all SNPs (single nucleotide polymorphism) in a predetermined section of a chromosome to define chromosome regions of prognostic value. Based on the SNPs in specified genes, a more accurate prognosis for breast cancer may be provided. | 06-18-2009 |
| 20090155806 | Genomic Sequence of the 5-Lipoxygenase-Activating Protein (FLAP), Polymorphic Markers Thereof and Methods for Detection of Asthma - The invention concerns the genomic sequence of the FLAP gene. The invention also concerns biallelic markers of a FLAP gene and the association established between these markers and diseases involving the leukotriene pathway such as asthma. The invention provides means to determine the predisposition of individuals to diseases involving the leukotriene pathway as well as means for the diagnosis of such diseases and for the prognosis/detection of an eventual treatment response to agents acting on the leukotriene pathway. | 06-18-2009 |
| 20090155807 | C-MET MUTATIONS IN LUNG CANCER - The invention provides methods and compositions useful for detecting mutations in c-met in lung cancer cells. | 06-18-2009 |
| 20090155808 | Automated system for isolating, amplifying and detecting a target nucleic acid sequence - A system and method for preparing and testing of targeted nucleic acids is presented. The system integrates a pipetter, extractor, assay reader, and other components, including a selectively compliant articulated robot arm (SCARA). This synergistic integration of previously separate diagnostic tools creates a system and method whereby a minimum of human intervention is required. The resulting system provides a substantially more accurate and precise method of isolating, amplifying and detecting targeted nucleic acids for diagnosing diseases. | 06-18-2009 |
| 20090155809 | SUBTRACTIVE SINGLE LABEL COMPARATIVE HYBRIDIZATION - Provided are methods of determining differences between nucleic acids in a test sample and a reference sample. In certain embodiments the methods are used for detecting and mapping chromosomal or genetic abnormalities associated with various diseases or with predisposition to various diseases, or to detecting the phenomena of large scale copy number variants. In particular, provided are advanced methods of performing array-based comparative hybridization that allow reproducibility between samples and enhanced sensitivity by using the same detectable label for both test sample and reference sample nucleic acids. Invention methods are useful for the detection or diagnosis of particular disease conditions such as cancer, and detecting predisposition to cancer based on detection of chromosomal or genetic abnormalities and gene expression level. Invention methods are also useful for the detection or diagnosis of hereditary genetic disorders or predisposition thereto, especially in prenatal samples. Moreover, invention methods are also useful for the detection or diagnosis of de novo genetic aberrations associated with post-natal developmental abnormalities. | 06-18-2009 |
| 20090162835 | Novel methods of constructing libraries comprising displayed and/or expressed members of a diverse family of peptides, polypeptides or proteins and the novel libraries - Methods useful in constructing libraries that collectively display and/or express members of diverse families of peptides, polypeptides or proteins and the libraries produced using those methods. Methods of screening those libraries and the peptides, polypeptides or proteins identified by such screens. | 06-25-2009 |
| 20090162836 | Prognostic and Diagnostic Markers for Cell Proliferative Disorders of The Breast Tissues - The present invention relates to prognostic and diagnostic markers for cell proliferative disorders of the breast tissues. The present invention therefore provides methods and nucleic acids for the analysis of biological samples for features associated with the development of breast cell proliferative disorders. Furthermore, the invention provides for prognosis of treatment effects relating to drug therapy, in particular hormonal/antihormonal therapy, chemotherapy and/or adjuvant therapy. | 06-25-2009 |
| 20090162837 | Inactivated FCV vaccines - The present invention relates to improved inactivated feline calicivirus (FCV) vaccines. The invention also provides a process for producing stabilized inactivated FCV, and the use of such stabilized inactivated FCV, in the production of FCV immunogenic compositions. The invention further provides methods of inducing an immune response in an animal of the Felidae family, preferably a cat, using the immunogenic compositions according to the invention. | 06-25-2009 |
| 20090162838 | FLUORESCENCE RESONANCE ENERGY TRANSFER ENZYME SUBSTRATES - Disclosed are compounds of formula (I) wherein D | 06-25-2009 |
| 20090162839 | DIAGNOSIS AND PROGNOSIS OF CANCER BASED ON TELOMERE LENGTH AS MEASURED ON CYTOLOGICAL SPECIMENS - The present invention concerns a quantitative in situ assessment of mean telomere length, particularly in relation to nuclear area, for the diagnosis and/or prognosis of cancer. In particular aspects, the methods and compositions regard diagnosis and/or prognosis of bladder cancer, urothelial cancer, lung cancer, and lymphoma. | 06-25-2009 |
| 20090162840 | Methods and compositions for use in analyte detection using proximity probes - Methods and compositions for detecting an analyte in a sample are provided. In practicing the subject methods, a sample is combined with at least a pair of proximity probes that each include an analyte binding domain and a nucleic acid domain. The resultant mixture is then contacted with a pair of asymmetric nucleic acid connectors. Proximity dependent connector mediated interaction between the nucleic acid domains of the proximity probes is then detected to determine the presence of the analyte in the sample. Also provided are kits and systems for practicing the subject methods. | 06-25-2009 |
| 20090162841 | Method of selecting a desired protein from a library - Described is an improved method of selecting a member of a specific binding pair (sbp) having a desired specify, preferably an antibody, from a library expressing said member of a sbp, preferably a phage-display antibody library. | 06-25-2009 |
| 20090162842 | Circulating mRNA as diagnostic markers - Methods and kits are provided for diagnosing, monitoring, or predicting the conditions of pre-eclampsia, fetal chromosomal aneuploidy, and pre-term labor in a pregnant woman, as well as for detecting pregnancy in a woman, by quantitatively measuring in the maternal blood the amount of one or more mRNA species encoding human chorionic gonadotropin β subunit (hCG-β), human placental lactogen (hPL), human corticotropin releasing hormone (hCRH), KiSS-1 metastasis-suppressor (KISS1), tissue factor pathway inhibitor 2 (TPFI2), placenta-specific 1 (PLAC1), or glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and comparing the amount of the mRNA species with a standard control. | 06-25-2009 |
| 20090162843 | IDENTIFICATION OF HPV16 LINEAGE GROUP - A method for identification of an HPV16 lineage group in a sample, comprising contacting such nucleic acid simultaneously with three probes, each probe being capable of specific hybridization across positions 143 and 145 of a HPV 16 genome. | 06-25-2009 |
| 20090162844 | Identification and characterization of racemases, definition of protein signatures, and a test for detecting D-amino acid and for screening molecules capable of inhibiting the activity of racemase, especially proline racemase - This invention provides identification and characterization of racemases and definition of protein signatures of these racemases. This invention also provides identification of nucleic acid molecules encoding a peptide consisting of a motif characteristic of the protein signatures, and to the peptides consisting of these motifs. Antibodies specific for the peptides and to immune complexes of these antibodies with the peptides are also provided. Further, the invention relates to methods and kits for detecting racemases using the nucleic acid molecules of the invention, as well as the peptides consisting of the motifs and antibodies to these peptides. | 06-25-2009 |
| 20090162845 | Affinity tag nucleic acid and protein compositions, and processes for using same - The present invention concerns compositions and processes that use affinity tags for isolating, and detecting or quantifying analytes, including nucleic acids, proteins and polypeptides. Compositions include nucleic acid compositions and protein compositions with affinity binding pairs, including metal binding peptides and immobilized metals, or peptide affinity groups. | 06-25-2009 |
| 20090162846 | SENP1 as a marker for cancer - The present invention provides methods of detecting cancer cells by detecting the quantity of SENP1 and/or telomerase in a sample. | 06-25-2009 |
| 20090162847 | RAPID DETECTION OF THE "HIGH-VIRULENT" ST-17 CLONE OF GROUP B STREPTOCOCCUS - The present invention relates to polynucleotides enabling the rapid, simple and specific detection of Group B | 06-25-2009 |
| 20090162848 | Noxin, a novel stress-induced gene involved in cell cycle and apoptosis - The invention provides for novel noxin polypeptides and nucleic acids from humans, rats, and mice and to related compositions and uses. The invention also provides for the creation of non-human, transgenic mammals which have, incorporated in their genome, DNA that includes a sequence of a mammalian noxin gene that does not produce noxin polypeptides, i.e. a noxin knockout mouse. | 06-25-2009 |
| 20090162849 | IDENTIFICATION OF A JAK2 MUTATION IN POLYCYTHEMIA VERA - The present invention concerns the V617F variant of the protein-tyrosine kinase JAK2, said variant being responsible for Vaquez Polyglobulia. The invention also relates to a first intention diagnostic method for erythrocytosis and thrombocytosis allowing their association with myeloproliferative disorders, or to the detection of the JAK2 V617F variant in myeloproliferative disorders allowing their reclassification in a new nosological group. | 06-25-2009 |
| 20090162850 | NUCLEIC ACID AND CORRESPONDING PROTEIN ENTITLED 125P5C8 USEFUL IN TREATMENT AND DETECTION OF CANCER - A novel gene (designated 125P5C8) and its encoded protein, and variants thereof, are described wherein 125P5C8 exhibits tissue specific expression in normal adult tissue, and is aberrantly expressed in the cancers listed in Table I. Consequently, 125P5C8 provides a diagnostic, prognostic, prophylactic and/or therapeutic target for cancer. The 125P5C8 gene or fragment thereof, or its encoded protein, or variants thereof, or a fragment thereof, can be used to elicit a humoral or cellular immune response; antibodies or T cells reactive with 125P5C8 can be used in active or passive immunization. | 06-25-2009 |
| 20090162851 | DETECTION OF NUCLEIC ACIDS BY TYPE SPECIFIC HYBRID CAPTURE METHOD - Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but is also highly specific and capable of discriminating highly homologous nucleic acid target sequences. The method produces DNA-RNA hybrids which can be detected by a variety of methods. | 06-25-2009 |
| 20090162852 | BAALC EXPRESSION AS A DIAGNOSTIC MARKER FOR ACUTE LEUKEMIA - Overexpression of the gene, BAALC, in biological samples from a patient is prognostic for tumor aggressiveness and unfavorable patient outcome. The present invention provides polynucleotide primers and probes for assaying for overexpression of BAALC transcripts. Kits containing the primers and probes are also provided. Also provided are antibodies for assaying for overexpression of BAALC proteins as well as peptide immunogens for producing the anti-BAALC antibodies. The present invention also provides methods for characterizing acute myelogenous leukemia, chronic myelogenous leukemia and prostate cancer in a patient, base on detection of BAALC overexpression. | 06-25-2009 |
| 20090162853 | METHODS AND DEVICES FOR CELLULAR ANALYSIS - Embodiments of the present invention are directed to improved methods and devices for analyzing a cell, aggregated cells, or a solid tumor. Such methods and devices are, for example, useful in the field of pathology and can provide improved cell processing and analytical results. | 06-25-2009 |
| 20090162854 | METHODS AND KITS FOR DIAGNOSIS OF SCHIZOPHRENIA - The present invention provides methods and kits for the diagnosis of schizophrenia, which employ mitochondrial complex I as a peripheral biological marker for schizophrenia. In an embodiment of the invention, the present invention provides a method for diagnosing schizophrenia in a subject by determining the level of m-RNA or protein mitochondrial complex I subunits and its activity by determining the cellular basal respiration through complex I enzyme. | 06-25-2009 |
| 20090162855 | Identification of Genetic Variants Associated with Increased Severity of Pulmonary Disease - A method of determining a genetic component contributing to the severity of a pulmonary disease in a patient comprises determining the presence or absence of one or more single nucleotide polymorphisms (SNPs) in the Endothelin Receptor A (EDNRA) gene or the Interleukin-8 (IL-8) gene of the patient. The SNPs are rs5335 or rs1801708 for EDNRA, or rs4O73 for IL-8. The pulmonary disease may be cystic fibrosis or lymphangioleimyomatosis. Determining the presence or absence of one or more of SNPs rs5335 or rs1801708 in the EDNRA gene or rs4073 in the IL-8 gene of the patient may also be used in a method of treating a patient having a pulmonary disease. A kit may comprise one or more probes for determining the presence or absence of one or more of SNPs rs5335 and rs1801708 in the EDNRA gene or the SNP rs4073 in the IL-8 gene. | 06-25-2009 |
| 20090162856 | RNA DETECTION METHOD - It is an object of the present invention to provide a method for rapid, convenient, and highly sensitive detection of trace RNA wherein a risk of contamination is low. The present invention provides a method for amplification of nucleic acid which comprises the steps of: (i) allowing a reverse transcriptase to act on RNA so as to produce a nucleic acid fragment; and (ii) performing substantially isothermal incubation of a reaction solution containing at least one type of deoxynucleotide triphosphate, at least one type of DNA polymerase having strand displacement activity, a divalent cation, a surfactant accounting for at least 0.01% of the solution, at least two types of oligonucleotide primers, and the nucleic acid fragment as a template obtained in the step (i) so as to perform a polymerase reaction that is initiated from the 3′ ends of the primers and thus amplify the nucleic acid fragment. | 06-25-2009 |
| 20090162857 | Pharmaceutical Compositions and Methods for Delivering Nucleic Acids Into Cells - The present invention relates to methods of delivering nucleic acids into cells using a nucleic acid binding molecule containing a multimeric or spacer-incorporated protein transduction domain (PTD). The invention also relates to novel compositions that contain a nucleic acid complexed or conjugated with a nucleic acid binding molecule. The nucleic acid binding molecule may contain a multimeric or spacer-incorporated PTD, and may further contain a nucleic acid binding region. The nucleic acid complexes or conjugations of the present invention may be employed to inhibit expression of a target gene, and/or determine the function of a target gene. | 06-25-2009 |
| 20090162858 | Orthogonal chemical inducer of dimerization - A method for identifying a molecule as being able to bind a protein target in a cell, comprising (a) covalently bonding the molecule to trimethoprim (TMP) to form a screening molecule, (b) introducing the screening molecule into the cell which (A) expresses (i) a first fusion protein comprising a binding domain capable of binding TMP, and (ii) a second fusion protein comprising the protein target, and (B) comprises a reporter gene, wherein one of the fusion proteins comprises a DNA-binding domain and the other fusion protein comprises a transcription activation domain and wherein expression of the reporter gene is conditioned on the proximity of the DNA-binding domain to the transcription activation domain; and (c) determining whether the screening molecule binds to the first fusion protein and to the second fusion protein by determining whether the cell expresses the reporter gene, wherein expression of the reporter gene indicates that the molecule binds the protein target and wherein lack of expression of the reporter gene indicates that the molecule does not bind the protein target. | 06-25-2009 |
| 20090162859 | Compositions, methods and systems for inferring canine breeds for genetic traits and verifying parentage of canine animals - Methods and systems are provided for managing companion animal subjects in order to maximize their individual health and potential performance and to maximize profits obtained in breeding and marketing the companion animal subjects. The methods and systems draw an inference of a phenotype for a genetic trait of a companion animal subject by determining the nucleotide occurrence of at least one companion animal SNP that is determined to be associated with the phenotype. The methods and systems described can be utilized to identify individual animals, determine or verify parentage of a single dog from any breed if the putative parent(s) are also available for testing, and are associated with, and predictive of, canine breeds. The inference is used in some aspects to diagnose a health condition or predisposition of a companion animal subject. | 06-25-2009 |
| 20090162860 | Method of nucleic acid sequence detection and nucleic acid sequence detection substrate - According to an aspect of the present invention, a pair of oligonucleotide strands are anchored onto the surface of a substrate by immobilizing one of the ends thereof onto the substrate. Each of the immobilized oligonucleotide strands is bound to a target nucleic acid sequence (single-stranded) having complementary sequences thereto to form a cross-linked structure on the substrate, thereby forming a finely reticulated space. Ligands are captured by this reticulated space through physical adsorption and caused to color with active substances reactive to the ligands. As a result of this, the present invention is capable of highly sensitively detecting even an exceedingly small concentration of a particular target nucleic acid sequence to be detected, at low cost and for a short time. | 06-25-2009 |
| 20090162861 | METHOD FOR SUPPRESSING A FRET SIGNAL, FRET SIGNAL SUPPRESSOR AGENTS AND USE IN A METHOD FOR MULTIPLEXING BIOLOGICAL EVENTS - The invention relates to a method for suppressing the FRET emitted by a reaction medium containing a pair of fluorescent FRET partner conjugates specific for a biological event, characterized in that a FRET signal killer which does not disturb said biological event is introduced into this medium. | 06-25-2009 |
| 20090162862 | DEVICE AND METHOD FOR THE AUTOMATED AND REPRODUCIBLE PRODUCTION OF CELL OR TISSUE SAMPLES THAT ARE TO BE ANALYZED AND ARE ARRANGED ON OBJECT SUPPORTS - An automatically operating apparatus designed as a table-top apparatus for the reproducible production of cell or tissue samples to be examined and arranged on specimen slides comprises, located its center, a rotatably supported, advance device having arranged on its periphery a plurality of modular processing stations at a distance from the advance device. Receiving devices provided in the peripheral region of the advance device are disposed to receive specimen slides on which automatically segmented cell and tissue segments can be positioned in a reproducible and correctly aligned manner. The cell and tissue segments are durably fixed in position on the specimen slides with the use of a curable adhesive, and the specimen slides, after being delivered, are subjected to further treatment processes in a further treatment device. | 06-25-2009 |
| 20090162863 | Nucleic acid detection probe - It is intended to provide a nucleic acid detection probe that is designed with a high degree of flexibility. The present invention provides a nucleic acid detection probe used in nucleic acid detection, wherein the amount of a particular target gene present in a sample is examined by simultaneously hybridizing, to the target nucleic acid, a first probe labeled at one end with a fluorophore and a second probe labeled with a quencher at an end different from the labeled end of the first probe and observing quenching attributed to the interaction between the fluorophore and the quencher. | 06-25-2009 |
| 20090162864 | BIOLOGICAL SUBSTANCE DETECTION CARTRIDGE,BIOLOGICAL SUBSTANCE DETECTION APPARATUS, AND BIOLOGICAL SUBSTANCE DETECTION METHOD - A biological substance detection cartridge, including: a reaction vessel for reacting a probe with a specific biological substance included in a sample solution, the reaction vessel having a region for fixing the probe for detecting the biological substance; a porous membrane facing the inside of the reaction vessel; a gas-liquid separation membrane superposed with the porous membrane; and a air discharge component which is provided on the opposite side of the gas-liquid separation membrane from the side contacting the porous membrane, and with which the interior can be kept at negative pressure during the reaction between the biological substance and the probe. | 06-25-2009 |
| 20090162865 | BREAST CANCER RELATED PROTEIN, GENE ENCODING THE SAME, AND METHOD OF DIAGNOSING BREAST CANCER USING THE PROTEIN AND GENE - An isolated protein having an amino acid sequence of SEQ ID No. 4 and having an activity inducing apoptosis, and a gene encoding the same are provided. Also, a microarray having a substrate on which the gene or fragment thereof is immobilized is provided. Also, a method of diagnosing breast cancer using an antibody specifically binding to the protein and a method of diagnosing breast cancer by determining whether the gene is expressed in a cell or not, are provided. | 06-25-2009 |
| 20090162866 | COMPOSITIONS AND METHODS FOR OBTAINING NUCLEIC ACIDS FROM SPUTUM - The present invention relates to compositions and methods for preserving and extracting nucleic acids from saliva. The compositions include a chelating agent, a denaturing agent, buffers to maintain the pH of the composition within ranges desirable for DNA and/or RNA. The compositions may also include a reducing agent and/or antimicrobial agent. The invention extends to methods of using the compositions of the invention to preserve and isolate nucleic acids from saliva as well as to containers for the compositions of the invention. | 06-25-2009 |
| 20090162867 | MUTATIONAL PROFILES IN HIV-1 REVERSE TRANSCRIPTASE CORRELATED WITH PHENOTYPIC DRUG RESISTANCE - The invention provides novel mutations, mutation combinations or mutational profiles of HIV-1 reverse transcriptase and/or protease genes correlated with phenotypic resistance to HIV drugs. More particularly, the present invention relates to the use of genotypic characterization of a target population of HIV and the subsequent correlation of this information to phenotypic interpretation in order to correlate virus mutational profiles with drug resistance. The invention also relates to methods of utilizing the mutational profiles of the invention in databases, drug development, i.e., drug design, and drug modification, therapy and treatment design, clinical management and diagnostic analysis. | 06-25-2009 |
| 20090170073 | FLUORESCENT PROTEIN AND CHROMOPROTEIN - It is an object of the present invention to provide a novel fluorescent protein and a novel chromoprotein. The present invention provides a novel fluorescent protein derived from | 07-02-2009 |
| 20090170074 | SYSTEM AND METHOD FOR NUCLEIC ACID SEQUENCING BY POLYMERASE SYNTHESIS - This invention relates to improved methods for sequencing and genotyping nucleic acid in a single molecule configuration. The method involves single molecule detection of fluorescent labeled PPi moieties released from NTPs as a polymerase extension product is created. | 07-02-2009 |
| 20090170075 | Methods of diagnosing or treating prostate cancer using the erg gene, alone or in combination with other over or under expressed genes in prostate cancer - The present invention relates to oncogenes or tumor suppressor genes, as well as other genes, involved in prostate cancer and their expression products, as well as derivatives and analogs thereof. Provided are therapeutic compositions and methods of detecting and treating cancer, including prostate and other related cancers. Also provided are methods of diagnosing and/or prognosing prostate cancer by determining the expression level of at least one prostate cancer-cell-specific gene, including, for example, the ERG gene or the LTF gene alone, or in combination with at least one of the AMACR gene and the DD3 gene. | 07-02-2009 |
| 20090170076 | Polynucleotides for the detection of escherichia coli 0157:h7 and escherichia coli 0157:nm verotoxin producers - Polynucleotide primers and probes for the specific detection of | 07-02-2009 |
| 20090170077 | Method for detecting recombinant event - Methods relating to isolating and amplifying chimeric nucleic acid molecules are provided. The methods of the invention are useful for detecting chromosome translocation events associated with diseases or conditions, such as cancer. | 07-02-2009 |
| 20090170078 | METHOD OF QUANTITATIVELY ANALYSING MICROORGANISM TARGETING RRNA - An object of the present invention is to provide a method of quantitating or detecting a microorganism, capable of detecting a live microorganism at high sensitivity and more precisely. A method of quantitating a microorganism of interest, using as an index the amount of rRNA of the microorganism of interest is provided. | 07-02-2009 |
| 20090170079 | Regulation of RUNX1 for Treatment of Pain - Methods are provides for identifying candidate agents for use in inhibiting expression of certain receptors and ion channels in nociceptors. Also provided are methods for identifying candidates agents for use in inhibiting neurophathic and other types of pain. | 07-02-2009 |
| 20090170080 | IDENTIFICATION OF BETA-PAPILLOMAVIRUS DNA BY TYPE-SPECIFIC REVERSE HYBRIDIZATION - The present invention relates human papilloma virus (HPV), more specific cutaneous, beta-PV. A method is disclosed for typing of any beta-PV nucleic acid with at least one probe capable of specific hybridization within the A region of beta-PV, said region being indicated in FIG. | 07-02-2009 |
| 20090170081 | GENE METHYLATION IN BLADDER CANCER DIAGNOSIS - The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with bladder cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use the detection and diagnosis of bladder cancer. | 07-02-2009 |
| 20090170082 | GENE METHYLATION IN RENAL CANCER DIAGNOSIS - The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with renal cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use the detection and diagnosis of renal cancer. | 07-02-2009 |
| 20090170083 | GENE METHYLATION IN DIAGNOSIS OF MELANOMA - The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with melanoma. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use the detection and diagnosis of melanoma. | 07-02-2009 |
| 20090170084 | GENE METHYLATION IN BREAST CANCER - The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with breast cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use the detection and diagnosis of breast cancer. | 07-02-2009 |
| 20090170085 | Gene Methylation in Head and Neck Cancer Diagnosis - The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with head and neck cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use the detection and diagnosis of head and neck cancer. | 07-02-2009 |
| 20090170086 | Gene Methylation In Esophageal Cancer Diagnosis - The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with esophageal cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use the detection and diagnosis of esophageal cancer. | 07-02-2009 |
| 20090170087 | Gene Methylation in Cervical Cancer Diagnosis - The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with cervical cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use the detection and diagnosis of cervical cancer. | 07-02-2009 |
| 20090170088 | GENE METHYLATION IN CANCER DIAGNOSIS - The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use the detection and diagnosis of cancer. | 07-02-2009 |
| 20090170089 | METHODS AND COMPOSITIONS FOR DIFFERENTIATING TISSUES OR CELL TYPES USING EPIGENETIC MARKERS - The present invention provides, inter alia, a method for generating a genome-wide epigenomic map, comprising a correlation between methylation variable CpG positions (MVP) and genomic DNA sample types. MVP are those CpG positions that show a variable quantitative level of methylation between sample types. Particular genomic regions of interest (ROI) provide preferred marker sequences that comprise multiple, and preferably proximate MVP, and that have novel utility for distinguishing sample types. The epigenic maps have broad utility, for example, in identifying sample types, or for distinguishing between and among sample types. In a preferred embodiment the epigenomic map is based on methylation variable regions (MVP) within the major histocompatibility complex (MHC), and has utility, for example, in identifying the cell or tissue source of a genomic DNA sample, or for distinguishing one or more particular cell or tissue types among other cell or tissue types. Analysis of epigenetic characteristics of one, or of a set of nucleic acid sequences, in the context of an inventive epigenomic map, allows for the determination of an origin of the nucleic acids. | 07-02-2009 |
| 20090170090 | Method for Enhancing Enzymatic DNA Polymerase Reactions - The invention relates to a method of enhancing a DNA polymerase reaction by including in a reaction mixture containing a DNA polymerase a protein of DNA ligase. | 07-02-2009 |
| 20090170091 | Method For Predicting Biological Systems Responses - The inventive method employs a “systems biology” approach to predicting biological responses resulting from exposure to the test substance. In one embodiment, the invention provides an automated method for predicting the biological systems effects of a test substance. In another embodiment, the invention provides a method for constructing a knowledgebase (or database) of response profiles for reference substances with known biological systems effects. In another embodiment, the invention provides a set of protocols and software tools used to carry out the profiling. Another embodiment of the invention is a panel of reagents and protocols required for generating response profiles, either to create an knowledgebase, or to use with an existing knowledgebase and informatics software to profile substance physiological effects. Another embodiment of the invention is a database of physiological profiles. | 07-02-2009 |
| 20090170092 | INTEGRATED MICROFLUIDIC ANALYSIS SYSTEMS - The present invention provides an integrated microfluidic analysis system. The system contains at least a first (pre-reaction treatment) domain for treating a sample prior to subjecting the sample to a chemical reaction. The following domains are optionally added to the first domain: a second (reaction) domain for reacting the chemical of interest in the sample; and a third (post-reaction separation) domain for separating products and reactants coming out of the reaction domain. The integrated microfluidic analysis system of the present invention is most applicable to PCR analysis. | 07-02-2009 |
| 20090170093 | SELECTION MARKER SYSTEM AND METHOD FOR SCREENING A CHOLINE TOLERANT PLANT CELL - The present invention relates to a selection marker system for distinguishing genetically modified plant cells from wild-type plant cells comprising (a) a nucleic acid sequence coding for choline oxidase, choline monooxygenase and/or choline dehydrogenase for genetically modifying at least a part of a plant and; (b) at least a part of a wild-type plant of the same plant species, wherein the part of the plant which is genetically modified by the nucleic acid sequence coding for choline oxidase, choline monooxygenase and/or choline dehydrogenase is capable of surviving in a medium containing choline at a concentration which is toxic to the wild-type plant. Furthermore, the invention relates to a method for screening and/or identifying a choline tolerant plant cell and the use of a nucleic sequence coding for a choline oxidase, choline monooxygenase and/or choline dehydrogenase as a selection marker for choline tolerance. | 07-02-2009 |
| 20090170094 | FLUORESCENT LABELING SUBSTANCE COMPRISING NANOPARTICLES OR NANORODS - A fluorescent labeling substance that is capable of realizing highly appropriate labeling through enhancing of the luminous efficiency of semiconductor nanoparticles or nanorods. The fluorescent labeling substance can be provided by disposing on a surface of shell of nanorods or nanoparticles having a modification group capable of adsorption with a biosubstance, such as protein, nucleic acid or antibody, a region devoid of the above modification group. | 07-02-2009 |
| 20090170095 | Mnk KINASE HOMOLOGOUS PROTEINS INVOLVED IN THE REGULATION OF ENERGY HOMEOSTASIS AND ORGANELLE METABOLISM - The present invention discloses Mnk homologous proteins regulating the energy homeostasis, the metabolism of triglycerides, and/or is contributing to membrane stability and/or function of organelles, and polynucleotides, which identify and encode the proteins disclosed in this invention. The invention also relates to the use of these sequences in the diagnosis, study, prevention, and treatment of diseases and disorders related to body-weight regulation and thermogenesis, for example, but not limited to, metabolic diseases such as obesity, as well as related disorders such as eating disorder, cachexia, diabetes mellitus, hypertension, coronary heart disease, hypercholesterolemia, dyslipidemia, osteoarthritis, gallstones, and sleep apnea, and disorders related to ROS defence, such as diabetes mellitus, neurodegenerative disorders, and cancer, e.g. cancers of the reproductive organs, and others. | 07-02-2009 |
| 20090170096 | NUCLEIC ACID AMPLIFICATION METHOD - An object to be achieved by the present invention is to provide a nucleic acid amplification method by which a nucleic acid can be amplified substantially isothermally using oligonucleotide primers and DNA polymerase capable of strand displacement. The present invention provides a nucleic acid amplification method which comprises performing substantially isothermal incubation of a reaction solution containing at least one type of deoxynucleotide triphosphate, at least one type of DNA polymerase having strand displacement activity, a divalent cation, at least 0.01% or more surfactant, at least two types of oligonucleotide primer, and the nucleic acid fragment as a template so as to perform a polymerase reaction that initiates from the 3′ end of the primer and thus amplifying the nucleic acid fragment. | 07-02-2009 |
| 20090170097 | GENE EXPRESSION IN BIOLOGICAL CONDITIONS - The present invention relates to a method of predicting the prognosis of a biological condition in animal tissue, wherein the expression of genes is examined and correlated to standards. The invention further relates to the treatment of the biological condition and an assay for predicting the prognosis. In particular, the invention concerns gene expression in epithelial tissue, such as urinary bladder under both normal and abnormal conditions. | 07-02-2009 |
| 20090170098 | ANTIBODIES TO CELL-CYCLE REGULATORY PROTEIN P16, AND USES RELATED THERETO - The present invention relates to the discovery in eukaryotic cells, particularly mammalian cells, of a family of cell-cycle regulatory proteins (“CCR-proteins”). As described herein, this family of proteins includes a polypeptide having an apparent molecular weight of 16 kDa, and a polypeptide having an apparent molecular weight of approximately 15 kDa, each of which can function as an inhibitor of cell-cycle progression, and therefore ultimately of cell growth. The present invention comprises antibodies directed to such CCR-proteins. The present invention is directed to a kit for detecting the level of cyclin-dependent kinase inhibitor p16 gene expression comprising antibodies directed to a p16 protein. | 07-02-2009 |
| 20090170099 | Use of Regulatory Sequences for Specific, Transient Expression in Neuronal Determined Cells - The present invention relates to the use of regulatory sequences for mediating specific, early transient expression in proliferative neuronal determined cells. Furthermore, the uses of recombinant nucleic acid molecules comprising said defined regulatory sequences for mediating specific, early transient expression in proliferative neuronal determined cells as well as for the generation of non-human transgenic organisms and/or host cells are disclosed. In addition, the invention provides for transgenic non-human animals and/or host cells comprising said regulatory sequences and/or recombinant nucleic acid molecules. The invention also describes methods for the preparation of such vectors, host cells and transgenic non-human animals as well as methods for the detection and/or isolation of neuronal determined cells. Additionally, methods for screening of compounds capable of regulating neuronal determined cell activity, neurogenesis, stimulating proliferation of neuronally committed precursor cells and/or neuronal differentiation are provided and the invention also relates to methods for the detection and analysis of neuronal differentiation, neuronal migration and/or neuronal determination processes. Finally, the invention relates to diagnostic and pharmaceutical compositions comprising the regulatory sequences, recombinant nucleic acid molecules, host-cells or isolated neuronal determined cells described herein. | 07-02-2009 |
| 20090170100 | Biomarkers and detection methods for gastric diseases - The present invention provides a biomarker for detecting gastric diseases, especially gastric cancer selected from: a nucleic acid sequence of GroES, complementary strand, or derivatives thereof or an amino acid sequence of GroES, derivatives, fragments or variants thereof or antibodies against said amino acid sequences or combinations thereof, yet provides a kit for detecting gastric cancer by use of above-mentioned biomarkers and a detection method. | 07-02-2009 |
| 20090170101 | IDENTIFICATION OF THYMICALLY DERIVED CD4 T CELLS BY PROTEIN TYROSINE KINASE 7 EXPRESSION - The invention provides methods of identifying naïve T cells by expression of PTK7. | 07-02-2009 |
| 20090170102 | METHODS FOR DETECTING DNA ORIGINATING FROM DIFFERENT INDIVIDUALS - In a first aspect, the present invention features methods for differentiating DNA species originating from different individuals in a biological sample. These methods may be used to differentiate or detect fetal DNA in a maternal sample or to differentiate DNA of an organ donor from DNA of an organ recipient. In preferred embodiments, the DNA species are differentiated by observing epigenetic differences in the DNA species such as differences in DNA methylation. In a second aspect, the present invention features methods of detecting genetic abnormalities in a fetus by detecting fetal DNA in a biological sample obtained from a mother. In a third aspect, the present invention features methods for differentiating DNA species originating from an organ donor from those of an organ recipient. In a fourth aspect, the present invention features kits for differentiating DNA species originating from different individuals in a biological sample. | 07-02-2009 |
| 20090170103 | OLIGONUCLEOTIDE SEQUENCES AND DNA CHIP FOR IDENTIFYING FILAMENTOUS MICROORGANISMS AND THE IDENTIFICATION METHOD THEREOF - A DNA chip for identifying filamentous microorganisms, including a substrate and a plurality of probes, wherein the probe includes SEQ ID No.1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, complementary sequences thereof, derivatives thereof or combinations thereof. The derivative is 5′ and/or 3′ end of the sequence SEQ ID No. 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or 16 with at least one thymidine residue added or is 5′ and/or 3′ end of the sequence SEQ ID No. 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or 16 with one or two nucleotides added or deleted. | 07-02-2009 |
| 20090170104 | COMPOSITIONS AND METHODS FOR DETECTING GROUP A STREPTOCOCCI - Compositions, methods and kits for detecting Group A streptococci. Particularly described are oligonucleotides that are useful as amplification primers and hybridization probes for detecting very low levels of Group A streptococci nucleic acids. | 07-02-2009 |
| 20090170105 | Diagnostics for Aging-Related Dermatologic Disorders - The present invention provides methods for the early prediction of aging-related dermatologic conditions of including skin changes associated with intrinsic aging or skin damages caused by extrinsic aging such as photoaging. The present invention also provides kits for the early determination of the propensity to develop such disorder and conditions. The method consists of detecting the presence of one or more alleles of an IL-1 haplotype or pattern, specifically the IL-1RN (+2018) and the IL-1B (−511) loci. The presence of allele 2 at the IL-1RN (+2018) and the IL-1B (−511) loci indicates decreased risk for a early onset of aging related dermatologic conditions. | 07-02-2009 |
| 20090170106 | ANALYSIS OF DNA - The invention provides improved techniques for investigating DNA samples, which offers improved sensitivity and specifity. | 07-02-2009 |
| 20090170107 | DETECTION ASSAY FOR MEAT AND BONE MEAL IN FEED - The invention concerns methods, kits and nucleic acids involved in detecting animal tissues, for example processed animal proteins (PAPs) or meat and bone meal (MBM), especially in feeds. In one method, a nucleic acid such as DNA is extracted from a sample using a process involving incubating the sample in an incubation buffer, autoclaving the incubated sample, and then mixing the sample with a metal-chelating agent. DNA extracted in this way may then be subjected to amplification using PCR or real-PCR, for example using primer and probe sequences as set forth in SEQ ID NOs 1-21. | 07-02-2009 |
| 20090170108 | Screening method, a composition comprising substances chosen in the method thereof, and a binding substance - (Problems) The main object of the present invention is to provide a screening method for selecting a substance affecting a bond between thioredoxin and MIF. | 07-02-2009 |
| 20090170109 | CORN EVENT TC1507 AND METHODS FOR DETECTION THEREOF - The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize TC1507 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided. | 07-02-2009 |
| 20090170110 | COMPOSITIONS AND METHODS TO DETECT CANDIDA ALBICANS NUCLEIC ACID - Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, capture probes for sample preparation, and detection probes specific for | 07-02-2009 |
| 20090170111 | GENETIC POLYMORPHISMS ASSOCIATED WITH STROKE, METHODS OF DETECTION AND USES THEREOF - The present invention is based on the discovery of genetic polymorphisms that are associated with vascular diseases, particularly stroke. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, variant proteins encoded by such nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and proteins, and methods of using the nucleic acid and proteins as well as methods of using reagents for their detection. | 07-02-2009 |
| 20090170112 | Loci Associated Charcoal Rot Drought Complex Tolerance in Soybean - The invention relates to methods and compositions for identifying soybean plants that are tolerant, have improved tolerance or are susceptible to Charcoal Rot Drought Complex. The methods use molecular genetic markers to identify, select and/or construct tolerant plants or identify and counter-select susceptible plants. Soybean plants that display tolerance or improved tolerance to Charcoal Rot Drought Complex that are generated by the methods of the invention are also a feature of the invention. | 07-02-2009 |
| 20090170113 | Non-Invasive Fetal Genetic Screening by Digital Analysis - The present methods are exemplified by a process in which maternal blood containing fetal DNA is diluted to a nominal value of approximately 0.5 genome equivalent of DNA per reaction sample. Digital PCR is then be used to detect aneuploidy, such as the trisomy that causes Down Syndrome. Since aneuploidies do not present a mutational change in sequence, and are merely a change in the number of chromosomes, it has not been possible to detect them in a fetus without resorting to invasive techniques such as amniocentesis or chorionic villi sampling. Digital amplification allows the detection of aneuploidy using massively parallel amplification and detection methods, examining, e.g., 10,000 genome equivalents. | 07-02-2009 |
| 20090170114 | Non-Invasive Fetal Genetic Screening by Digital Analysis - The present methods are exemplified by a process in which maternal blood containing fetal DNA is diluted to a nominal value of approximately 0.5 genome equivalent of DNA per reaction sample. Digital PCR is then be used to detect aneuploidy, such as the trisomy that causes Down Syndrome. Since aneuploidies do not present a mutational change in sequence, and are merely a change in the number of chromosomes, it has not been possible to detect them in a fetus without resorting to invasive techniques such as amniocentesis or chorionic villi sampling. Digital amplification allows the detection of aneuploidy using massively parallel amplification and detection methods, examining, e.g., 10,000 genome equivalents. | 07-02-2009 |
| 20090176205 | Mutant proteins in the dna-binding domain of a telomeric protein, trf2, and telomeric dna mutants, and use of the complex structure of the trf2 dna-binding domain and telomeric dna - A TRF2 DNA-binding domain mutant protein comprising:
| 07-09-2009 |
| 20090176206 | Toll-like receptor 2 (tlr-2) haplotypes predict outcome of patients - The invention provides methods and kits for obtaining a prognosis for a subject having or at risk of developing an inflammatory condition and or a gram positive infection. The method generally includes determining a toll-like receptor 2 (TLR-2) risk genotype of a subject for one or more SNPs, comparing the determined genotype with known genotypes for the polymorphism that correspond with the ability of the subject to recover from the inflammatory condition and identifying subjects based on their prognosis. | 07-09-2009 |
| 20090176207 | Methods for Determining Risk of Developing Regular Smoking Behavior - The present invention relates in general to human genetic polymorphisms and their association with human health and to methods and materials for analyzing allelic variations, and to the use of genetic polymorphisms in the diagnosis and treatment of smoking behavior and nicotine dependence. Provided herein are methods for determining risk in a subject of developing regular smoking behavior. Also provided are primers, probes, microarrays, and kits related thereto. | 07-09-2009 |
| 20090176208 | Methods for detecting, identifying and reporting the presence of animal pathological agents - A business method for use in detecting the presence of one or more pathological agents in a biological sample taken from an animal, using known diagnostic methods including antibodies and PCR, marketing test kits for obtaining and transporting biological samples from animals to a processing center, and reporting the results of diagnosis of biological samples from one or more animals. | 07-09-2009 |
| 20090176209 | Selection - The present invention to a nucleotide sequence encoding one or more Arc DNA binding domains, one or more Arc DNA binding sites and at least one polypeptide domain. | 07-09-2009 |
| 20090176210 | MICROBIAL REDUCTIVE DEHALOGENATION OF VINYL CHLORIDE - Compositions and methods are provided that relate to the bioremediation of chlorinated ethenes, particularly the bioremediation of vinyl chloride by | 07-09-2009 |
| 20090176211 | FLUORESCENT PROTEIN - It is an object of the present invention to provide a fluorescent protein, which allows an acceptor for fluorescence resonance energy transfer (FRET) to appear in a stimulating light-dependent manner, thereby enabling the marking of any given cell organelle, cells, or tissues, with multiple colors. The present invention provides a fluorescent protein which consists of a fused protein of a donor fluorescent protein and an acceptor fluorescent protein, wherein before irradiation with stimulating light, the donor protein is able to emit fluorescence as a result of irradiation of the donor protein with excitation light; and after irradiation with stimulating light, intramolecular FRET occurs between the donor fluorescent protein and the acceptor fluorescent protein as a result of irradiation of the donor protein with excitation light, and the acceptor protein is able to emit fluorescence, and wherein the fluorescence of the donor protein and the fluorescence of the acceptor protein have wavelengths that are different from each other. | 07-09-2009 |
| 20090176212 | Method for Fixing a Supercoiled DNA and the Use for Analyzing the DNA Repair - A method for fixing a supercoiled DNA consists in deposing a supercoiled DNA sample on the surface of a porous polymer film, in fixing said supercoiled DNA therein by a passive diffusion, in obtaining a support by the inventive method and in using said support for analysing the DNA distribution. | 07-09-2009 |
| 20090176213 | STABILIZATION OF SOLID SUPPORT ASSAY REAGENTS - Reagents are disclosed for use in assays for analytes. The reagents are dry assay reagents that may be readily reconstituted for use in the assays. The dry assay reagents comprise a solid support and one or more molecules of a receptor immobilized on the solid support. The receptor comprises one or more binding sites for a ligand. A portion of a total number of the binding sites is bound to a conjugate comprising the ligand linked to a specific binding pair member and a portion of the total number of the binding sites is free. In use in an assay, a combination is provided in an aqueous medium comprising the sample and reagents for detecting the analyte wherein at least one of the reagents comprises the dry assay reagent mentioned above. The combination is incubated under conditions for binding of the analyte to one or more of the reagents. The presence and/or amount of binding of the analyte to one or more of the reagents is detected where the presence and/or amount of the binding is related to the presence and/or amount of the analyte in the sample. | 07-09-2009 |
| 20090176214 | Recombinant antigens for the detection of Coxiella burnetii - The invention relates to a method for the detection of prior exposure to | 07-09-2009 |
| 20090176215 | GENE METHYLATION IN PROSTATE CANCER DIAGNOSIS - The present invention provides DNA biomarker sequences that are differentially methylated in samples from normal individuals and individuals with prostate cancer. The invention further provides methods of identifying differentially methylated DNA biomarker sequences and their use the detection and diagnosis of prostate cancer. | 07-09-2009 |
| 20090176216 | Alleles and polymorphisms predictive of responsiveness to biologic therapy in psoriasis - The invention provides methods for predicting responsiveness to a biologic agent in a subject suffering from psoriasis. The methods involve assaying for the presence or absence of an allele or polymorphism in the subject that is predictive of responsiveness to biologic therapy, such as an HLA-Cw6 allele, a TNFα 238 polymorphism or a TNFα 308 polymorphism. The methods can further comprise selecting a treatment regimen with a biologic agent in a psoriasis subject based upon presence or absence of the allele or polymorphism in the subject. The methods can further comprise administering a biologic agent to the subject according to the selected treatment regimen. Kits that include means for assaying an allele or polymorphism that is predictive of responsiveness to biologic therapy for psoriasis are also provided. Methods of preparing and using databases, and computer program products therefore, for selecting a psoriasis subject for treatment with a biologic agent are also provided. | 07-09-2009 |
| 20090176217 | NOVEL NUCLEOTIDE AND AMINO ACID SEQUENCES, AND ASSAYS AND METHODS OF USE THEREOF FOR DIAGNOSIS - Novel splice variants, amino acid sequences and nucleotide sequences thereof, and methods of using same. | 07-09-2009 |
| 20090176218 | Method for the Diagnosis and Prognosis of Cancer - Disclosed are methods for prognostic evaluation, and diagnosis of cancers. The methods of the present invention allow for the diagnosis and prognosis of cancers by analyzing the levels of expression of an enzyme that is involved in the biosynthetic pathway of spermine within tissue samples containing cancerous tissues. In particular, the amount of enzyme mRNA detected in tissue sample correlates with disease status such that enzyme levels can be used to predict the presence of, as well as the metastatic potential of cancer. Thus, measuring the level of spermine pathway enzymes in biological samples provides a quick, easy, and safe screen that can be used to both diagnose and prognose cancer in a patient thus leading to more effective treatments and cures. | 07-09-2009 |
| 20090176219 | DNA BINDING SITE OF A TRANSCRIPTIONAL ACTIVATOR USEFUL IN GENE EXPRESSION - We have discovered DNA binding sites which are specifically recognized by PrtT, a transcriptional activator for protease genes. The DNA binding site can be defined structurally by a consensus nucleotide sequence and functionally by PrtT's ability to regulate transcriptional activation through that sequence. Both PrtT and its cognate DNA binding site (i.e., the nucleotide sequence in each promoter that is recognized by PrtT) can be used in a gene expression system. Possession of only a PrtT transcriptional activator is insufficient, its cognate DNA binding site is necessary for recognition by PrtT (i.e., binding to the site and activating transcription under appropriate conditions). A functional site, such as one obtained from a wild-type fungal gene, will confer PrtT-dependent transcriptional activation on 3′-downstream sequences. A mutation of a wild-type promoter that results in a non-functional site will abolish PrtT-dependent transcriptional activation of 3′-downstream sequences. A mutation of a wild-type promoter that results in a more functional site will enhance PrtT-dependent transcriptional activation of 3′-downstream sequences. | 07-09-2009 |
| 20090176220 | ANTIBODY CHARACTERIZATION TEST - The present invention relates to a method for measuring the ability of an antibody preparation to activate an Fc receptor, wherein this method comprises the following steps: a) aggregating said antibodies with one another, b) bringing cells expressing an Fc receptor into contact with said aggregated antibodies, and c) measuring the reaction of the cells resulting from the activation of the Fc receptor of said cells by the Fc region of said antibodies. | 07-09-2009 |
| 20090176221 | METHOD OF DETECTING AN ANALYTE IN A SAMPLE USING SEMICONDUCTOR NANOCRYSTALS AS DETECTABLE LABEL - The use of semiconductor nanocrystals as detectable labels in various chemical and biological applications is disclosed. The methods find use for detecting a single analyte, as well as multiple analytes by using more than one semiconductor nanocrystal as a detectable label, each of which emits at a distinct wavelength. | 07-09-2009 |
| 20090176222 | Gene for Identifying Individuals with Familial Dysautonomia - This invention relates to methods and compositions useful for detecting mutations which cause Familial Dysautonomia. Familial dysautonomia (FD; Riley-Day syndrome), an Ashkenazi Jewish disorder, is the best known and most frequent of a group of congenital sensory neuropathies and is characterized by widespread sensory and variable autonomic dysfunction. Previously, we mapped the FD gene, DYS, to a 0.5 cM region of chromosome 9q31 and showed that the ethnic bias is due to a founder effect, with >99.5% of disease alleles sharing a common ancestral haplotype. To investigate the molecular basis of FD, we sequenced the minimal candidate region and cloned and characterized its 5 genes. One of these, IKBKAP, harbors two mutations that can cause FD. The major haplotype mutation is located in the donor splice site of intron 20. This mutation can result in skipping of exon 20 in the mRNA from FD patients, although they continue to express varying levels of wild-type message in a tissue-specific manner. RNA isolated from patient lymphoblasts is primarily wild-type, whereas only the deleted message is seen in RNA isolated from brain. The mutation associated with the minor haplotype in four patients is a missense (R696P) mutation in exon 19 that is predicted to disrupt a potential phosphorylation site. Our findings indicate that almost all cases of FD are caused by an unusual splice defect that displays tissue-specific expression; and they also provide the basis for rapid carrier screening in the Ashkenazi Jewish population. | 07-09-2009 |
| 20090176223 | Compositions, Kits, and Methods for the Diagnosis, Prognosis, and Monitoring of Immune Disorders Using Galectin-1 - The present invention is based, in part, on the discovery that galectin-1 (Gal1) plays a role in immune disorders, including Hodgkin lymphoma, anaplastic large cell lymphoma, or MLL | 07-09-2009 |
| 20090176224 | Udder Health Characteristics - The invention relates to a method for determining udder health characteristics in bovine subjects, wherein udder health characteristics comprise sub-clinical and clinical mastitis. In particular, the method of the invention involves identification of genetic markers and/or Quantitative Trait Locus (QTL) for the determination of udder health characteristics in a bovine subject. The determination of udder health characteristics involves resolution of the specific microsatellite status. Furthermore, the invention relates to a diagnostic kit for detection of genetic marker(s) associated with udder health. The method and kit of the present invention can be applied for selection of bovine subjects for breeding purposes. Thus, the invention provides a method of genetically selecting bovine subjects with udder health characteristics that will yield cows less prone to mastitis. | 07-09-2009 |
| 20090176225 | DIAGNOSTIC TEST FOR COLLIE EYE ANOMALY - The invention relates to a method for identifying dogs which are genetically normal, heterozygous for, or homozygous for the mutation primarily responsible for Collie eye anomaly (CEA). The method comprises the steps of obtaining a biological sample from a dog and testing DNA in the biological sample for the presence or absence of a 7.8 kilobase deletion within chromosome 37 in which the CEA mutation is located. No deletion is indicative of a normal dog. A deletion on one allele of chromosome 37 is indicative of a dog that is heterozygous for the CEA mutation. A deletion in both alleles of chromosome 37 are indicative of a dog that is homozygous for the CEA mutation. Also provided is a kit for identifying a dog as normal, heterozygous for, or homozygous for the CEA mutation. | 07-09-2009 |
| 20090176226 | METHOD FOR DIAGNOSING AUTISM SPECTRUM DISORDER - The present invention provides methods of diagnosing and/or predicting autism spectrum disorder comprising determining the presence of microdeletions and microduplications on chromosomes 15 and 16. | 07-09-2009 |
| 20090176227 | Method for Producing Polyploid Plants of Orchids - A method for producing polyploid plants of orchids includes the steps of: providing a protocorm or protocom-like body (PLB) of an orchid, the protocorm or PLB having an upper portion with a growing point and a lower portion without any growing point; cutting the protocorm or PLB approximately at a point of half height to separate the upper portion; subculturing the lower portion of the protocorm or PLB in an inducing medium, and putting a cut surface of the lower portion of the protocorm or PLB to face upward so that one or more next-generation PLBs grow from the cut surface of the lower portion. The method characterized in using no antimicrotubule agent can simplify the entire process of orchid polyploidy breeding, and can be used in mass-production of the stable polyploid plants. | 07-09-2009 |
| 20090176228 | LUNG CANCER MARKERS, AND USES THEREOF - Methods and compositions are provided for assessing (e.g., diagnosing), treating, and preventing diseases, especially cancer, and particular lung cancer, using lung cancer markers (LCM). Individual LCM and panels comprising multiple LCM are provided for these and other uses. Methods and compositions are also provided for determining or predicting the effectiveness of a treatment or for selecting a treatment using LCM. Methods and compositions are further provided for modulating cell function using LCM. Also provided are compositions that modulate LCM (e.g., antagonists or agonists), such as antibodies, proteins, small molecule compounds, and nucleic acid agents (e.g., RNAi and antisense agents), as well as pharmaceutical compositions thereof. Further provided are methods of screening for agents that modulate LCM, and agents identified by these screening methods. | 07-09-2009 |
| 20090176229 | Methods and compositions for the identification of insect repellent compounds - Methods for identifying a candidate compound with an ability to modulate cation transport through a transient receptor potential (TRP) channel in a cell are disclosed. The methods can include (a) providing a cell expressing a recombinant nucleic acid sequence encoding an transient receptor potential (TRP) channel gene product or a functional fragment or derivative thereof; (b) contacting the cell with the candidate compound; (c) comparing cation transport in the cell in the absence of the candidate compound with cation transport in the cell in the presence of the candidate compound; and (d) identifying a candidate compound through the comparing step that modulates cation transport in the cell through the transient receptor potential (TRP) channel. Also disclosed are nucleic acid and amino acid sequences for insect TRP channel gene products, antibodies that bind to the disclosed TRP channels, and recombinant host cells the include the disclosed biosequences. | 07-09-2009 |
| 20090176230 | Microfluidic device and methods of using same - A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses. | 07-09-2009 |
| 20090176231 | PROBE FOR DETECTING ABL GENE MUTATION AND USES THEREOF - Detection probes are provided that are capable of detecting a sequence to be detected containing a mutation even when a sequence not to be detected containing no mutation coexists with the sequence to be detected containing a mutation, which are different only in a single base from each other. At least one oligonucleotide selected from the group consisting of SEQ ID NOs: 2˜16 is used as a probe. Even in a sample containing an abl gene in which a mutation has occurred and an abl gene in which no mutation has occurred, the use of such probes in, for example, Tm analysis allows the mutation to be detected. | 07-09-2009 |
| 20090176232 | ASSESSMENT OF REACTION KINETICS COMPATIBILITY BETWEEN POLYMERASE CHAIN REACTIONS - A method compares amplification reaction kinetics between two or more quantitative polymerase chain reactions (PCR). These methods enable quality control and/or quality assessment for quantification of nucleic acids by PCR. The method estimates plurality of parameters from each reaction and compares them simultaneously between reactions. | 07-09-2009 |
| 20090176233 | Screening assays for polymerase enhancement - Methods of screening for and selecting for improved polymerases suitable for single molecule sequencing are provided. | 07-09-2009 |
| 20090176234 | Efficient base determination in sequencing reactions - The present invention is directed to compositions and methods for nucleic acid identification and detection. Compositions and methods of the present invention include extracting and fragmenting target nucleic acids from a sample, using the fragmented target nucleic acids to produce target nucleic acid templates and subjecting those target nucleic acid templates to amplification methods to form nucleic acid nanoballs. The invention also includes methods of detecting and identifying sequences using various sequencing applications, including sequencing by ligation methods. | 07-09-2009 |
| 20090176235 | GENETIC POLYMORPHISMS ASSOCIATED WITH MYOCARDIAL INFARCTION, METHODS OF DETECTION AND USES THEREOF - The present invention is based on the discovery of genetic polymorphisms that are associated with myocardial infarction. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, variant proteins encoded by such nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and proteins, and methods of using the nucleic acid and proteins as well as methods of using reagents for their detection. | 07-09-2009 |
| 20090176236 | Compositions and Methods for Detecting Certain Flaviviruses, Including Members of the Japanese Encephalitis Virus Serogroup - The present invention provides rapid and accurate methods, primers, probes and kits for identifying the presence of a certain flaviviruses in a sample. Flaviviruses that can be detected include members of the Japanese encephalitis virus serogroup, Dengue virus, St. Louis encephalitis virus, Montana myotis leukoencephalitis virus, Modoc virus, and Yellow Fever virus. The primers and probes of the invention can hybridize to regions in the 3′ untranslated region of the viral genomes to be detected. | 07-09-2009 |
| 20090176237 | USE OF MICRO-RNA AS A BIOMARKER OF IMMUNOMODULATORY DRUG ACTIVITY - Methods of determining the activity of an immunomodulatory compound by measuring the presence of an miRNA in a sample are disclosed. Additionally disclosed are methods of assessing the patient compliance in patients treated with an immunomodulatory compound. | 07-09-2009 |
| 20090176238 | Cynomolgus Toll-Like Receptor 3 - Isolated polynucleotides encoding Cynomolgus monkey Toll-Like Receptor 3 (cynoTLR3), polypeptides obtainable from expression of these polynucleotides, recombinant cells, methods and uses of these are disclosed. | 07-09-2009 |
| 20090176239 | SINGLE-MOLECULE-FORMAT REAL-TIME BIOLUMINESCENCE IMAGING PROBE - The present invention provides an “in vivo and in vitro real-time bioluminescence imaging means,” which can transmit a detection signal promptly in response to an external signal, while taking advantage of a single-molecule-format luminescent probe as a bioluminescent means. | 07-09-2009 |
| 20090176240 | MN/CA IX and Cancer Prognosis - Herein disclosed are methods that are prognostic for neoplastic/preneoplastic disease in a subject vertebrate, wherein said disease is associated with a tissue that normally expresses MN, but which MN expression is lost or diminished upon carcinogenesis. Exemplary of the types of preneoplastic/neoplastic diseases subject to the prognostic methods of this invention are those of gastric mucosa, gallbladder, biliary ducts, and ductal cells of duodenal glands. An exemplary prognostic method comprises comparing the level of MN gene expression product in a tissue sample from the affected subject, with the average MN gene expression product level found in analogous preneoplastic/neoplastic tissue samples; an above average MN gene expression product level indicates poorer prognosis for the subject. MN gene expression products useful in the prognostic methods include MN protein, MN polypeptide, and/or MN nucleic acids. | 07-09-2009 |
| 20090176241 | COMPOSITIONS AND METHODS FOR DETECTING GROUP B STREPTOCOCCI - Compositions, methods and kits for detecting Group B streptococci. Particularly described are oligonucleotides that are useful as amplification primers and hybridization probes for detecting very low levels of Group B streptococci nucleic acids. | 07-09-2009 |
| 20090176242 | COMPOSITIONS AND METHODS FOR LABELING OF NUCLEIC ACID MOLECULES - The present invention is generally related to compositions, kits and methods for labeling nucleic acid molecules using reverse transcriptases, preferably multi-subunit reverse transcriptases such as ASLV reverse transcriptases. Specifically, the invention relates to methods, kits and compositions for fluorescently labeling nucleic acid molecules during nucleic acid synthesis. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection and diagnostics. | 07-09-2009 |
| 20090181366 | INTERNAL POSITIVE CONTROL FOR NUCLEIC ACID ASSAYS - Compositions and methods for detecting a non-specific nucleic acid amplification inhibitor in a reaction are disclosed. An internal positive control (IPC) may be included in samples to be tested for target nucleic acids as a means to monitor non-specific inhibition of nucleic acid amplification and provide confidence in negative results obtained in target-specific assays. Provided herein are an IPC polynucleotide, IPC control primers, and IPC probes. Also provided are methods of using an IPC polynucleotide, primers, and probes to detect a non-specific nucleic acid amplification inhibitor. | 07-16-2009 |
| 20090181367 | Diagnosis of sepsis using mitochondrial nucleic acid assays - The invention provides assays to detect sepsis disease states in a subject by determining the relative amount of mitochondrial nucleic acid in the subject. The assays of the invention may include PCR assays, such semi-quantitative or quantitative PCR involving the co-amplification of a mitochondrial sequence and a reference sequence, such as a genomic sequence. Information from such assays may be evaluated to provide a ratio of mitochondrial nucleic acid to nuclear nucleic add in the cells of the subject. | 07-16-2009 |
| 20090181368 | METHOD OF DETECTING MYCOPLASMA - An object of the present invention is to provide a primer capable of detecting a mycoplasma | 07-16-2009 |
| 20090181369 | Methods for the Treatment of Disease - The present invention is directed to methods to determine the likelihood of therapeutic effectiveness of a farnesyl transferase inhibitor (FTI). The method comprises determining whether the gene encoding the farnesyl transferase beta subunit (FNTB) of said patient comprises at least one nucleic acid variance that causes an alteration in an amino acid residue. The change in the amino acid residue is associated with resistance to a FTI. The absence of at least one variance indicates that the FTI is likely to be effective. | 07-16-2009 |
| 20090181370 | Method for Sequencing a Polynucleotide Template - The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which permit the sequential determination of nucleotide sequences in two distinct and separate regions on complementary strands of the double-stranded polynucleotide template. The two regions for sequence determination may or may not be complementary to each other. | 07-16-2009 |
| 20090181371 | METHODS AND COMPOSITIONS RELATED TO A BRAF MUTATION AND MICROSATELLITE STABILITY - Disclosed are methods and compositions related to a BRAF mutation and microsatellite stability. | 07-16-2009 |
| 20090181372 | Method and compositions for monitoring DNA binding molecules in living cells - The present invention provides a method of screening for a compound that binds to a selected nucleic acid comprising contacting compound fluorescently labeled by a fluorescent protein with a cell having a plurality of copies of the nucleic acid in an array such that the nucleic acid can be directly detected when bound by fluorescently labeled compound; and directly detecting the location of fluorescence within the cell, fluorescence aggregated at the site of the nucleic acid array indicating a compound that binds to the selected nucleic acid. In particular compounds such a transcription factors can be screened. Reagents for such method are provided including a mammalian cell having a plurality of steroid receptor response elements in an array such that the response element can be directly detected when bound by fluorescently labeled steroid receptor and a chimeric protein comprising a fluorescent protein fused to a steroid receptor. | 07-16-2009 |
| 20090181383 | Cancer Risk Biomarker - The present disclosure relates to methods and compositions for identifying biomarkers that indicate a biological state, in particular cancer or predisposition to cancer. Also provided are methods and compositions for identifying a greater risk for a cancer-related condition in an subject. | 07-16-2009 |
| 20090181384 | Prognosis prediction for colorectal cancer - This invention relates to prognostic signatures, and compositions and methods for determining the prognosis of cancer in a patient, particularly for colorectal cancer. Specifically, this invention relates to the use of genetic markers for the prediction of the prognosis of cancer, such as colorectal cancer, based on signatures of genetic markers. In various aspects, the invention relates to a method of predicting the likelihood of long-term survival of a cancer patient, a method of determining a treatment regime for a cancer patient, a method of preparing a treatment modality for a cancer patient, among other methods as well as kits and devices for carrying out these methods. | 07-16-2009 |
| 20090181385 | Reagents, methods, and libraries for bead-based sequencing - The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to immobilized beads. The invention further provides sets of labeled probes containing phosphorothiolate linkages. In addition, the invention includes performing multiple sequencing reactions on a single template by removing initializing oligonucleotides and extended strands and performing subsequent reactions using different initializing oligonucleotides. | 07-16-2009 |
| 20090181386 | Calving Characteristics - The invention relates to a method for determining calving characteristics in bovine subjects, wherein calving characteristics comprise stillbirth, calving difficulty and calf size at birth, which are all economically important factors. In particular, the method of the invention involves identification of genetic markers and/or Quantitative Trait Locus (QTL) for the determination of calving characteristics in a bovine subject. The determination of calving characteristics involves resolution of the specific microsatellite status. Furthermore, the invention relates to a diagnostic kit for detection of genetic marker(s) associated with calving characteristics. The method and kit of the present invention can be applied for selection of bovine subjects for breeding purposes. Thus, the invention provides a method of genetically selecting bovine subjects with calving characteristics that will yield cows less prone to stillbirth, calving difficulties and undesired calf size at birth. | 07-16-2009 |
| 20090181387 | Method for Analysing Nucleic Acids - Method of analyzing nucleic acids comprising the steps of nucleic acid fractionation, adaptor binding and nucleic acid amplification, and an in vitro transcription step. The invention has application in the field of genomic analysis of organisms by the use of DNA microarrays. | 07-16-2009 |
| 20090181388 | Enclosed unit for rapid detection of a target nucleic acid amplification product - The invention relates to a method for rapid detection of a target nucleic acid amplification product while preventing cross-contamination between target nucleic acid amplification products and avoiding false positives, comprising the steps of: a) leaving the reaction tube unopened after the amplification reaction is finished, so as to prevent the target nucleic acid amplification product from leaking out and resulting in contamination; b) placing the unopened reaction tube inside an enclosed unit, making the target nucleic acid amplification product be transferred to a test strip from the reaction tube in a physically enclosed environment; c) performing detection in a visual read-out manner, and determining the result; d) discarding the enclosed unit in a safety place as a whole without opening it after the detection. The invention also relates to a totally enclosed unit for detecting a target nucleic acid amplification product, and still relates to applications of the totally enclosed rapid detection unit in detection of infectious pathogens, food industry, agriculture, livestock husbandry, customs quarantine control, and determination of DNA. | 07-16-2009 |
| 20090181389 | QUANTITATIVE MEASUREMENT OF NUCLEIC ACID VIA LIGATION-BASED LINEAR AMPLIFICATION - Methods, compositions and kits are provided for sensitive and quantitative detection of nucleic acid, especially for the determination of the presence and/or amount of a target nucleic acid with mutations or single nucleotide polymorphism (SNP). In particular, assays are provided for amplifying a target nucleic acid via ligation of designed oligonucleotide probes and linear amplification by using an RNA polymerase, such as T7 polymerase. The assays can be used for diagnosis, prognosis or monitoring of diseases or disorders, for pharmacogenomic studies of patient stratification and drug responses, for discovery of therapeutic targets, or for forensic analysis. | 07-16-2009 |
| 20090181390 | HIGH THROUGHPUT DETECTION OF MICRORNAS AND USE FOR DISEASE DIAGNOSIS - Methods, compositions and kits are provided for high throughput detection of micro RNAs (miRNA), especially for sensitive and specific detection of miRNA that are in low abundance and closely related to each other. In particular, an assembly of designed oligonucleotide probes with unique tag sequences is used to achieve these purposes via high throughput microarrays, optionally in conjunction with branched-DNA based array detection. The assays can be used for diagnosis, prognosis or monitoring of diseases or disorders such as cancer, for pharmacogenomic studies of patient stratification and drug responses, for discovery of therapeutic targets, or for forensic analysis. | 07-16-2009 |
| 20090181391 | METHODS FOR ANALYSIS OF DNA METHYLATION PERCENTAGE - Methods are disclosed for determining the methylation state of DNA samples using melt analysis including high resolution melt analysis. Methods are also provided for determining methyltransferase activity using melt analysis including high resolution melt analysis. Additionally, kits of parts are provided. | 07-16-2009 |
| 20090181392 | Transgenic Zebrafish Models for Neurodegenerative Diseases - The present invention relates to zebrafish models for neurodegenerative disorders that allow screening of compounds for their ability to protect and/or regenerate neurons in vivo in a whole vertebrate organism. The present invention also provides methods of identifying gene targets for neuroprotective compounds, compounds that regenerate neurons and compounds that promote neurogenesis. | 07-16-2009 |
| 20090181393 | Methods for the identification, assessment, and treatment of patients with proteasome inhibition therapy - The present invention is directed to the identification of markers that can be used to determine whether patients with cancer are clinically responsive or non-responsive to a therapeutic regimen prior to treatment. In particular, the present invention is directed to the use of certain combinations of markers, wherein the expression of the markers correlates with responsiveness or non-responsiveness to a therapeutic regimen comprising proteasome inhibition. Thus, by examining the expression levels of individual markers and those comprising a marker set, it is possible to determine whether a therapeutic agent, or combination of agents, will be most likely to reduce the growth rate of tumors in a clinical setting. | 07-16-2009 |
| 20090181394 | Diagnostic methods and kits for hepatocellular carcinoma using comparative genomic hybridization - The invention provides diagnostic methods for determining the prognosis of hepatocellular carcinoma (HCC) comprising the steps of (a) observing recurrently altered genomic region on a chromosome; (b) measuring variation of one or more of RAR expression variations selected from the RAR variation group consists of gains of RAR-G1 to RAR-G14 and losses of RAR-L1 to RAR-L18 as defined in table 1, a diagnostic kit, and genes for useful in diagnosis or prognosis of liver cancer. | 07-16-2009 |
| 20090181395 | DETECTION OF ANTIBIOTIC-RESISTANT MICROORGANISMS - Method of detecting methicillin-resistant | 07-16-2009 |
| 20090181396 | METHODS AND SYSTEMS FOR ANALYSIS OF FLUORESCENT REACTIONS WITH MODULATED EXCITATION - Methods, systems and their components for monitoring fluorescent signals and particularly transient fluorescent signals from reaction mixtures of interest, which methods and systems employ modulated excitation light sources to reduce impacts of excessive illumination on the reaction components or the data obtained therefrom. | 07-16-2009 |
| 20090181397 | PREDICTIVE AND DIAGNOSTIC METHODS FOR CANCER - The present disclosure encompasses methods of diagnosing the presence of a cancer, and particularly a cancer of prostate or breast tissue, in a human subject, predicting the outcome or severity of the disease and methods of reversing the prostate cell transformation based on the presence or absence in the human subject of a dinucleotide (TT) deletion in the gene encoding the U50 snoRNA. Provided, therefore, are methods of identifying a genetic marker of a human subject indicating a cancerous tissue in the human subject, embodiments of the methods comprising: determining from an isolated nucleic acid sample the genotype of the human subject with respect to a locus encoding a snoRNA U50, where a mutation within the nucleotide sequence encoding a snoRNA U50, when compared with a wild-type nucleotide sequence encoding a snoRNA U50, identifies in the human subject a genetic marker associated with a cancer in the human subject. The cancer may be, but is not necessarily limited to, a prostate cancer or a breast cancer. | 07-16-2009 |
| 20090181398 | Nanoparticle conjugates - Conjugate compositions are disclosed that include a specific-binding moiety covalently coupled to a nanoparticle through a heterobifunctional polyalkyleneglycol linker. In one embodiment, a conjugates is provided that includes a specific-binding moiety and a fluorescent nanoparticle coupled by a heterobifunctional PEG linker. Fluorescent conjugates according to the disclosure can provide exceptionally intense and stable signals for immunohistochemical and in situ hybridization assays on tissue sections and cytology samples, and enable multiplexing of such assays. | 07-16-2009 |
| 20090181399 | INSECT RESISTANT COTON PLANTS AND METHODS OF DETECTING THE SAME - The present application relates to an insect resistant transgenic cotton event designated COT202. The application also relates to polynucleotides which are characteristic of the COT202 event, plants comprising said polynucleotides, and methods of detecting the COT202 event. | 07-16-2009 |
| 20090181400 | Method of Detection of Prostate Cancer - The present invention provides methods and kits useful for detecting neplasia by measuring the methylation level of biomarkers, especially the promoter region of GSTP1 for the detection of prostate adenocarcinoma. | 07-16-2009 |
| 20090181401 | Detection format for hot start real time polymerase chain reaction - The present invention is directed to a method and a composition for amplifying and detecting a target nucleic comprising subjecting said target nucleic acid to a real time PCR amplification reaction in the presence of a thermostable DNA polymerase, a thermostable double strand dependent 3′-5′ exonuclease having a temperature optimum above 37° C., a pair of amplification primers, deoxynucleoside triphosphates, a detecting oligonucleotide carrying a first label and a second label, said first label being capable of acting as a fluorescent reporter entity when excited with light of an appropriate wavelength, said second label being capable of acting as a fluorescence quenching entity of said fluorescent reporter entity, characterized in that one label is bound to the 3′ end of said detecting oligonucleotide, and further characterized in that the other label is bound either internally or at the 5′ end of said detecting oligonucleotide, and monitoring fluorescence of said fluorescent reporter entity at least after a plurality of amplification cycles. | 07-16-2009 |
| 20090186339 | Human transcriptomes - Global gene expression patterns have been characterized in normal and cancerous human cells using serial analysis of gene expression (SAGE). Cancer cell-specific, cell-type specific, and ubiquitously expressed genes have been identified. This information can be used to provide combinations of cell type- and cancer-specific gene probes, as well as methods of using these probes to identify particular cell types, screen for useful drugs, reduce cancer-specific gene expression, standardize gene expression, and restore function to a diseased cell or tissue. | 07-23-2009 |
| 20090186340 | Gene Analysis - This invention relates to a series of PCR primers that will allow the simultaneous amplification of regions of the clinically significant ABO and RHD genes. | 07-23-2009 |
| 20090186341 | Receptacle for the Separation of Tumor Cells - The invention describes a container ( | 07-23-2009 |
| 20090186342 | Methods of producing competitive aptamer fret reagents and assays - Methods are described for the production and use of fluorescence resonance energy transfer (FRET)-based competitive displacement aptamer assay formats. The assay schemes involve FRET in which the analyte (target) is quencher (Q)-labeled and previously bound by a fluorophore (F)-labeled aptamer such that when unlabeled analyte is added to the system and excited by specific wavelengths of light, the fluorescence intensity of the system changes in proportion to the amount of unlabeled analyte added. Alternatively, the aptamer can be Q-labeled and previously bound to an F-labeled analyte so that when unlabeled analyte enters the system, the fluorescence intensity also changes in proportion to the amount of unlabeled analyte. The F or Q is covalently linked to nucleotide triphosphates (NTPs), which are incorporated into the aptamer by various nucleic acid polymerases, such as Taq or Deep Vent Exo | 07-23-2009 |
| 20090186343 | METHODS FOR PREPARING MODIFIED BIOMOLECULES, MODIFIED BIOMOLECULES AND METHODS FOR USING SAME - A novel and efficient single pot synthetic schemes are disclosed for preparing modified nucleotides, nucleotide analogs, nucleotide polyphosphates, and nucleotide polyphosphate analogs. The novel method is used to prepare nucleotides, nucleotide analogs, nucleotide polyphosphates, and nucleotide polyphosphate analogs having non-persistent or persistent and non-persistent modifications. Novel biomolecular reactions are also disclosed using the novel modified biomolecules disclosed herein. | 07-23-2009 |
| 20090186344 | DEVICES AND METHODS FOR DETECTING AND QUANTITATING NUCLEIC ACIDS USING SIZE SEPARATION OF AMPLICONS - Devices and methods are described for detecting and quantifying nucleic acids using a sealed system that minimizes contamination. In particular, provided herein are devices for and methods using nucleic acid amplification that permit multiple sampling of an amplification reaction mixture and quantitation and identification of amplicons during the course of an amplification reaction. Methods involving the transfer of samples from an amplification reaction mixture into a separation network, separation of nucleic acids based on size, and identification and quantitation of nucleic acids are disclosed. | 07-23-2009 |
| 20090186345 | Instrument and method for nucleic acid isolation - According to the present invention, purified nucleic acids are isolated without the complete removal of components of a washing reagent. Also, nucleic acids are isolated in an elution reagent by allowing a washing reagent comprising at least one component constituting a reaction solution that can be applied to a reaction involving nucleic acids to come into contact with a nucleic-acid-adsorbing solid phase so as to wash the solid phase, separating the washing reagent from the solid phase in a manner such that a certain amount of the washing reagent is allowed to remain in the solid phase, and allowing an elution reagent to come into contact with the nucleic-acid-adsorbing solid phase. | 07-23-2009 |
| 20090186346 | Method for Detecting the Presence or Absence of a Target Cell in a Sample - The present invention relates to a method for detecting the presence or absence of a target cell in a sample, said method comprising (a) binding cells in said sample to a particulate and mixable solid support; (b) eluting the cells from the solid support without the use of competitor molecules to disrupt the interaction between the cell and the solid support; (c) after lysis of said cells, detecting the presence or absence of nucleic acid characteristic of said target cell, wherein said solid support does not have antibodies or antibody fragments immobilised thereon. Kits for carrying out the method of the invention are also provided. | 07-23-2009 |
| 20090186347 | Markers for metabolic syndrome - Correlations between polymorphisms and metabolic syndrome, insulin resistance, obesity, high blood pressure, dyslipidemia, diabetes and/or myocardial infarction are provided. Methods of diagnosing and treating metabolic syndrome, insulin resistance, obesity, high blood pressure, dyslipidemia, diabetes and/or myocardial infarction are provided. Systems and kits for diagnosis and treatment of metabolic syndrome, insulin resistance, obesity, high blood pressure, dyslipidemia, diabetes and/or myocardial infarction are provided. | 07-23-2009 |
| 20090186348 | MICRORNAS DIFFERENTIALLY EXPRESSED IN CERVICAL CANCER AND USES THEREOF - The present invention concerns methods and compositions for identifying a miRNA profile for a particular condition, such as cervical disease, and using the profile in assessing the condition of a patient. | 07-23-2009 |
| 20090186349 | DETECTION OF NUCLEIC ACID REACTIONS ON BEAD ARRAYS - The present invention is directed to methods and compositions for the use of microsphere arrays to detect and quantify a number of nucleic acid reactions. The invention finds use in genotyping, i.e. the determination of the sequence of nucleic acids, particularly alterations such as nucleotide substitutions (mismatches) and single nucleotide polymorphisms (SNPs). Similarly, the invention finds use in the detection and quantification of a nucleic acid target using a variety of amplification techniques, including both signal amplification and target amplification. The methods and compositions of the invention can be used in nucleic acid sequencing reactions as well. All applications can include the use of adapter sequences to allow for universal arrays. | 07-23-2009 |
| 20090186350 | Muitiforms suspension microgranular bioreactor and methods of use - A Multiform Suspension Microgranular Bioreactor (MSMB) is used to detect nucleic acids or peptides/proteins in molecular and biological samples. Polymer-based microgranules, coupled with biomass molecule probes, are constructed with different features. The biomass molecule probes include cDNA probes, oligonucleic acid probes, peptide or protein probes. These microgranules are differentiated according to their features including shape, size, color, fluorescence intensity, magnetic property, gravity, chemical luminal intensity, radioactivity, and other labels. The above microgranules are suspended in a sample solution and are used collectively as a microarray-like bioreactor device. Such MSMB-based microarrays are particularly useful in complicated biological detection assays with flow cytometry. | 07-23-2009 |
| 20090186351 | NUCLEOTIDE ANALOGS - The invention provides for nucleotide analogs and methods of using the same, e.g., for sequencing nucleic acids. | 07-23-2009 |
| 20090186352 | DNA Conformation - Method of detection or diagnosis of abnormal gene expression in an individual comprising determining in a sample from the individual the presence or absence of a chromosome structure in which two separate regions of the gene have been brought into close proximity, to thereby detect or diagnose whether the individual has abnormal gene expression. | 07-23-2009 |
| 20090186353 | CANCER-RELATED NUCLEIC ACIDS - Described herein are polynucleotides associated with specific types of cancers. The polynucleotides are miRNAs, miRNA precursors, and associated nucleic acids. Methods and compositions are described that can be used for diagnosis, prognosis, and treatment of various cancers. | 07-23-2009 |
| 20090186354 | METHODS AND TOOLS FOR THE SCREENING OF FACTORS AFFECTING PROTEIN MISFOLDING - The present invention relates in general to the fields of chemical, pharmaceutical and genetic screening and to diseases associated with protein misfolding. In particular, it discloses engineered cells and a system based thereon that can be used to screen for substances that affect protein misfolding. The engineered cells of the invention comprise one or more reporter genes under transcriptional control of a promoter that is responsive to protein misfolding. | 07-23-2009 |
| 20090186355 | Method for determination and quantification of radiation or genotoxin exposure - The present invention discloses methods for detecting exposure of a living subject to genotoxic agents, testing sensitivity to a genotoxic agent, and determining DNA damage caused by exposure to an agent, comprising detecting the presence of FANCD2-containing foci from a sample collected from said subject. The presence of concentrated foci is indicative of DNA damage, and the degree of foci formation is correlated with degree of exposure. Diagnostic reagents contain a ligand that binds to human FANCD2 associated with a detectable label. Kits for detecting DNA damage in a biological sample contain such diagnostic reagents and signal detection components. The invention further discloses methods for identifying agents which modulate the ability of FANCD2-containing foci to form. Among other things, such agents are potentially useful chemosensitizing agents or may confer protection against damage caused by genotoxic agents. | 07-23-2009 |
| 20090186356 | DEVICE AND SUBSTANCE FOR THE IMMOBILIZATION OF MESENCHYMAL STEM CELLS (MSCs) - The invention relates to a device comprising at least one surface which comes into contact with biological tissue and/or liquid, which is at least partially coated with a substance which mediates the binding of mesenchymal stem cells (MSCs), a method for the binding and/or isolation of MSCs from biological tissue and/or liquid, a nucleic acid molecule which selectively and highly specifically binds to MSCs, the use of the nucleic acid molecule for the binding and/or isolation of MSCs from biological tissue and/or liquid, as well as a method for the production of a device mentioned at the outset. | 07-23-2009 |
| 20090186357 | Integrated PCR Reactor for Cell Lysis, Nucleic Acid Isolation and Purification, and Nucleic Acid Amplication Related Applications - Disclosed are integrated devices capable of performing a polymerase chain reaction within a single vessel. Also disclosed are related methods of sample analysis. | 07-23-2009 |
| 20090186358 | Pathway Analysis of Cell Culture Phenotypes and Uses Thereof - The present invention provides methods for systematically identifying genes, proteins and/or related pathways that regulate or indicative of cell phenotypes. The present invention further provides methods for manipulating the identified genes, proteins and/or pathways to engineer improved cell lines and/or to evaluate or select cell lines with desirable phenotypes. | 07-23-2009 |
| 20090186359 | DETECTING PROSTATE CANCER - An assay for detecting prostate cancer includes reagents for detecting the methylation of GSTP1 and HIC-1 genes. | 07-23-2009 |
| 20090186360 | Detection of GSTP1 hypermethylation in prostate cancer - An assay for detecting prostate cancer includes reagents for detecting multiple methylation markers from within one gene such as GSTP1. | 07-23-2009 |
| 20090186361 | METHODS OF PREDICTING THE PHARMACEUTICAL TOXICITY OF TAXANES - The present disclosure relates to methods of identifying a recipient subject who has an elevated risk of an adverse or toxic reaction to a taxane. The predictive methods of the present disclosure allow for the recipient subject most at risk of adversely reacting to the taxane, to be administered alternative treatments or to have the dosage modified to reduce or eliminate toxic reactions. It has been discovered that recipient subjects bearing certain SNPs of the cytochrome CYP2C8 and TUBB genes have an elevated risk of toxicity from a taxane. The methods of the present disclosure encompass methods of calculating a Toxicity Index for a recipient subject based on clinical data from the recipient subject as it relates to the administration and reaction to treatment with taxane. This Toxicity Index value is then correlated to the presence of polymorphisms at a site in each of the gene loci CYP2C8 and TUBB, or a combination of both sites. | 07-23-2009 |
| 20090186362 | FUNGAL DELTA-12 DESATURASE AND DELTA-15 DESATURASE MOTIFS - The present invention relates to fungal Δ12 desaturases (responsible for conversion of oleic acid to linoleic acid (18:2, LA)) and Δ15 fatty acid desaturases (responsible for conversion of LA to α-linolenic acid (18:3, ALA)). Amino acid motifs diagnostic of Δ12 desaturases and Δ15 desaturases are also provided. Methods of altering enzyme specificity towards Δ12 desaturation or towards Δ15 desaturation and/or increasing production of specific ω-3 and ω-6 fatty acids by over-expression of the fungal desaturases are also described. | 07-23-2009 |
| 20090186363 | COMPOSITIONS AND METHODS FOR THE DIAGNOSIS AND TREATMENT OF TUMOR - The present invention is directed to compositions of matter useful for the diagnosis and treatment of tumor in mammals and to methods of using those compositions of matter for the same. | 07-23-2009 |
| 20090191541 | Nucleic Acid Molecules and Other Molecules Associated with Plants - The present invention is in the field of plant genetics. More specifically the invention relates to nucleic acid molecules and nucleic acid molecules that contain markers, in particular, single nucleotide polymorphism (SNP) and repetitive element markers. In addition, the present invention provides nucleic acid molecules having regulatory elements or encoding proteins or fragments thereof. The invention also relates to proteins and fragments of proteins so encoded and antibodies capable of binding the proteins. The invention also relates to methods of using the nucleic acid molecules, markers, repetitive elements and fragments of repetitive elements, regulatory elements, proteins and fragments of proteins. | 07-30-2009 |
| 20090191542 | Multi-variant cell indication technique - The invention provides methods for identifying and isolating cells that express high levels of protein. A method is described for identifying a cell or cell line expressing a desired protein comprising determining in a cell or cell line that has been transfected with a vector comprising a nucleotide sequence encoding the desired protein the intracellular expression levels of elements of the vector and also determining expression of the desired protein on the surface of the cell or cell line, in certain cases in a single assay. | 07-30-2009 |
| 20090191543 | IDENTIFICATION OF RAC1B AS A MARKER AND MEDIATOR OF METALLOPROTEINASE-INDUCED MALIGNANCY - The present invention provides compositions and methods for detecting MMP-induced malignancies by detecting Rac1b expression. The invention further provides compositions and in vitro and in vivo methods for inhibiting MMP-induced malignant transformation by modulating Rac1b expression and/or function. | 07-30-2009 |
| 20090191544 | Correlating chemical and spatial data within pathology samples - A tissue sample to be analyzed is tested. Multiple different samples of multiple areas of said tissue sample, each of said multiple areas is a smaller area than an entire tissue sample to be analyzed. After analysis, the tissue sample is processed in a way that facilitates viewing tumor information on the sample, for example, by fixing or staining. An area is identified on the stained sample, and at least one of the samples that corresponds to an identified area is tested. This small area is tested using a technique that homogenizes the sample before testing. | 07-30-2009 |
| 20090191545 | Selection of High-Producing Cell Lines - The invention describes a method for screening antibody producing cell lines for selection of high expressing clones. | 07-30-2009 |
| 20090191546 | ENGINEERED TOEHOLD REACTIONS AND NETWORKS - A catalytic system and method of catalyzing reactions that uses a novel toehold exchange mechanism that allows a specified input to catalyze the release of a specified output, which in turn can serve as a catalyst for other reactions is provided. This toehold exchange catalyst system, which can be driven forward by the configurational entropy of the released molecule, provides an amplifying circuit element that is simple, fast, modular, composable, and robust. Using this toehold exchange catalyst system it has been possible to construct and characterize several circuits that amplify nucleic acid signals, including a feed-forward cascade with quadratic kinetics and a positive feedback circuit with exponential growth kinetics. | 07-30-2009 |
| 20090191547 | Exon grouping analysis - This invention pertains to the identification of specific disease-causing DNA sequences in a subject. The methods of the present invention can be used to identify genetic alterations, to determine the molecular basis for genetic diseases, and to provide carrier and prenatal diagnosis for genetic counseling. | 07-30-2009 |
| 20090191548 | METHODS AND NUCLEIC ACIDS FOR THE ANALYSIS OF GENE EXPRESSION ASSOCIATED WITH TISSUE CLASSIFICATION - The present application provides methods and nucleic acids the classification of a biological sample. This is achieved by the analysis of the expression status of at least one of the genes selected from Table 1 as disclosed. | 07-30-2009 |
| 20090191549 | SINGLE NUCLEOTIDE POLYMORPHISMS (SNP) AND THEIR ASSOCIATION WITH TICK RESISTANCE IN BOVINE ANIMALS - A method for assessing tick resistance in a bovine animal, comprising the steps of:
| 07-30-2009 |
| 20090191550 | Diagnosis of whipple's disease - The invention relates to a method for in vitro seriological diagnosis of Whipple's disease, whereby the bacteria responsible for the disease are isolated and established in a culture and brought into contact with the serum or biological fluid of an infected patient. The invention also relates to useful oligonucleotides with a probe and a primer for amplifying, sequencing and detecting the gene rpoB of the bacteria, | 07-30-2009 |
| 20090191551 | Use of Secretor, Lewis and Sialyl Antigen Levels in Clinical Samples as Predictors of Risk for Disease - An individual at risk for necrotizing enterocolitis and related disorders can be identified by measuring the level of at least one secretor antigen in a biological sample from the individual and comparing the measured level of the at least one secretor antigen to a predetermined value or a predetermined range of values. Among the secretor antigens which can be measured are: the H-1, H-2, Lewis | 07-30-2009 |
| 20090191552 | Novel Human Acidic Mammalian Chitinase and Use Thereof - Described herein is a variant human acidic mammalian chitinase (AMCase) enzyme having improved stability and an isolated nucleic acid sequence which encodes the variant. The invention also provides to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing recombinant variant AMCase polypeptide and its use in screening assays to identify modulators. | 07-30-2009 |
| 20090191553 | Chase Ligation Sequencing - In various embodiments, the present teachings provide sequencing methods which facilitate enhancing the efficiency of ligation and/or increasing sequencing reads. Various embodiments of the methods enable sequencing through template regions for which complementary labeled extension probes are unavailable or insufficient. In various embodiments, one or more rounds of ligation with unlabeled extension probes can be used in addition to a round of ligation with labeled extension probe. In various embodiments, for example, such methods can facilitate extension on template polynucleotides that do not bind labeled extension probe in the first round of ligation. | 07-30-2009 |
| 20090191554 | MODIFIED GLASS FIBER WITH MONOLAYER OF AMINOCALIXARENE DERIVATIVES AND IMINECALIXARENE DERIVATIVES - Techniques for a surface-modified glass fiber with monolayer of aminocalixarene derivatives and iminecalixrene derivatives are provided. | 07-30-2009 |
| 20090191555 | HCV NS3-NS4 Protease Resistance Mutants - The present invention is directed to mutants of HCV NS3/4A protease. More particularly, the present invention identifies mutant of HCV NS3/4A protease that are resistant to drug treatment. | 07-30-2009 |
| 20090191556 | Method - The present invention relates to a novel method for the delivery of agents to tumour cells. In particular it relates to a method for the specific delivery of agents to the interior of tumour cells. Uses of the method are also described. | 07-30-2009 |
| 20090191557 | MN/CA IX/CA9 and renal cancer prognosis - Herein disclosed are methods that are prognostic for renal cell carcinoma, particularly renal clear cell carcinoma, afflicting a vertebrate. An exemplary prognostic method comprises detecting the presence of, and quantitating the level and/or extent of a MN/CA9 gene expression product in a sample from the affected subject, wherein if 50% or fewer cells are found to express the MN/CA9 gene, then the subject is considered to have a poorer prognosis. MN/CA9 gene expression products useful in the prognostic methods include MN protein, MN polypeptide and/or MN nucleic acids. The methods are useful as an aid in the selection of treatment for a patient with renal cell carcinoma, alone or in combination with conventional tumor stage and/or grade information. The methods of the invention can be used, for example, to identify those patients requiring more aggressive therapy regimens, or those patients most likely to respond to adjuvant immunotherapies, particularly MN/CA IX/CA9-targeted therapies. | 07-30-2009 |
| 20090191558 | DETECTION, IDENTIFICATION AND DIFFERENTIATION OF SERRATIA SPECIES USING THE SPACER REGION - The present invention relates to new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region, between the 16S and 23S rRNA genes, to be used for the specific detection and/or identification of | 07-30-2009 |
| 20090191559 | HER2 DIAGNOSTIC METHODS - In certain aspects, the present invention provides methods for determining whether a Her-2 positive cancer is likely to respond to treatment with a Her2-acting agent and/or whether a patient with a Her-2 positive cancer is likely to have a slow disease progression. In other aspects, the present invention is drawn to methods for determining whether a subject with a Her-2 positive cancer is unlikely to respond to treatment with at least one chemotherapeutic agent in addition to a Her2-acting agent and/or whether a patient with a Her-2 positive cancer is likely to have a fast disease progression. | 07-30-2009 |
| 20090191560 | MUTANT DNA POLYMERASES AND USES THEROF - The present invention relates to mutant DNA polymerases which incorporate dideoxynucleotides with about the same efficiency as deoxynucleotides. The present invention also related to mutant DNA polymerases which also have substantially reduced 5′-to-3′ exonuclease activity or 3′-to-5′ exonuclease activity. The invention also relates to DNA molecules coding for the mutant DNA polymerases, and hosts containing the DNA molecules. | 07-30-2009 |
| 20090191561 | METHODS AND OLIGONUCLEOTIDES FOR DETECTION OF MASTITIS CAUSING BACTERIA - The present invention relates to the field of polymerase chain reaction (PCR) based diagnostic assays. More specifically, the present invention provides a PCR-method for detecting mastitis causing bacteria, particularly | 07-30-2009 |
| 20090191562 | METHOD FOR ASSAYING REG IV mRNA - In an RNA amplification process comprising steps of using a first primer and a second primer, at least one of which has a promoter sequence at the 5′ end, and reverse transcriptase, to produce double-stranded DNA containing the promoter sequence, using the double-stranded DNA as template to produce an RNA transcript with RNA polymerase, and using the RNA transcript in turn as template for DNA synthesis with the reverse transcriptase to produce the double-stranded DNA, the amount of amplified RNA product is measured with an intercalating fluorescent dye-labeled nucleic acid probe. | 07-30-2009 |
| 20090191563 | UNIFORM FRAGMENTATION OF DNA USING BINDING PROTEINS - The invention provides a method for preparing and analysing a population of fragmented polynucleotide sequences having a substantially uniform size. The method can include steps of (a) binding at least some protection molecule to at least one polynucleotide sequence; (b) cleaving the at least one polynucleotide sequence to generate a plurality of polynucleotide fragment sequences of substantially uniform size; (c) amplifying the polynucleotide fragments; and (d) determining a sequence characteristic of a plurality of the polynucleotide fragments. | 07-30-2009 |
| 20090191564 | DIAGNOSTICS AND THERAPEUTICS FOR CARDIOVASCULAR DISORDERS - The methods of the present invention relate to the diagnosis of cardiovascular disorders. In one aspect, the specification discloses methods for determining a subject's predisposition to increased risk for myocardial infarction. | 07-30-2009 |
| 20090191565 | SHORT CYCLE METHODS FOR SEQUENCING POLYNUCLEOTIDES - The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or fill completion. | 07-30-2009 |
| 20090191566 | Kits for Isolating Nucleic Acids Using Multifunctional Group-Coated Solid Phase Carriers - The present invention is directed to a method of isolating a target species (e.g., target nucleic acid species) from a mixture. In the methods of the present invention, the mixture is combined with solid phase carriers having a surface comprising multiple functional groups one of which reversibly and selectively binds the target species. In a particular embodiment, the mixture is combined with solid phase carriers having a first functional group which reversibly binds nucleic acids and a second functional group which selectively and reversibly binds the target nucleic acid species, thereby producing a first combination. The first combination is maintained under conditions appropriate for binding of the nucleic acids to the first functional group and binding of the target nucleic acid species to the second functional group. The solid phase carriers are separated from the first combination, and combined with an agent (e.g., buffer) that selectively removes (e.g., elutes) either the nucleic acid from the first functional group or the target nucleic acid species from the second functional group of the solid phase carriers, thereby isolating a target nucleic acid species from a mixture comprising a plurality of nucleic acid species. | 07-30-2009 |
| 20090191567 | SEMICONDUCTOR NANOCRYSTAL PROBES FOR BIOLOGICAL APPLICATIONS AND PROCESS FOR MAKING AND USING SUCH PROBES - A semiconductor nanocrystal compound and probe are described. The compound is capable of linking to one or more affinity molecules. The compound comprises (1) one or more semiconductor nanocrystals capable of, in response to exposure to a first energy, providing a second energy, and (2) one or more linking agents, having a first portion linked to the one or more semiconductor nanocrystals and a second portion capable of linking to one or more affinity molecules. One or more semiconductor nanocrystal compounds are linked to one or more affinity molecules to form a semiconductor nanocrystal probe capable of bonding with one or more detectable substances in a material being analyzed, and capable of, in response to exposure to a first energy, providing a second energy. Also described are processes for respectively: making the semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and treating materials with the probe. | 07-30-2009 |
| 20090191568 | METHODS AND COMPOSITIONS FOR PREDICTING COMPLIANCE WITH AN ANTIDEPRESSANT TREATMENT REGIMEN - Methods and compositions are provided for predicting whether a subject will comply with an antidepressant treatment regimen. In practicing the subject methods, a subject's serotonin transporter gene-linked polymorphic region (5HTTLPR) is genotyped. Based on the identified genotype, a particular antidepressant treatment regimen suitable for the patient is determined. Also provided are kits for practicing the subject methods. | 07-30-2009 |
| 20090191569 | Mammalian Cytokines; Related Reagents - Purified genes encoding a cytokine or composite cytokine from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding these molecules are provided. Methods of using said reagents and diagnostic kits are also provided. | 07-30-2009 |
| 20090197246 | Haplotypes and polymorphisms linked to human thiopurine s-methyltransferase deficiencies - Haplotypes and polymorphisms of thiopurine S-methyltransferase (TPMT) are described that are linked to TPMT deficiencies which can cause potentially fatal toxicity when patients are treated with thiopurines like mercaptopurine, azathioprine, or thioguanine. The mutant alleles as well as PCR fragments, kits and methods for assaying the TPMT genotype of individual patients are disclosed. Furthermore, algorithms are disclosed that combine the genotypes of a set of single nucleotide polymorphisms to haplotypes that give a distinct information about the TPMT phenotype. | 08-06-2009 |
| 20090197247 | Composition for increasing microorganism wall permeability and method for detecting said microorganisms on a membrane - The present invention relates to a composition for permeabilizing microorganism walls, comprising the combination of polyethyleneimine (PEI) with at least one alcohol, and also to a method using said composition for counting and detecting in a targeted manner the microorganisms on a membrane. The invention also relates to a kit and to probes that are suitable for carrying out said method. | 08-06-2009 |
| 20090197248 | VITRO EVOLUTION IN MICROFLUIDIC SYSTEMS - The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention. | 08-06-2009 |
| 20090197249 | COMPOSITIONS AND METHODS FOR DIAGNOSING COLON DISORDERS - The present invention relates to methods and compositions for diagnosing, monitoring, prognosticating, analyzing, etc., polymicrobial diseases. The present invention also relates to the microbial community present in the digestive tract and lumen in normal subjects, and subjects with digestive tract diseases, especially diseases of the colon, such as inflammatory bowel disease, including ulcerative colitis, Crohn's syndrome, and pouchitis. The present invention especially relates to compositions and methods for diagnosing and prognosticating the mentioned diseases and conditions, e.g., to determine the presence of the disease in a subject, to determine a therapeutic regimen, to determine a therapeutic regimen, to determine the onset of active disease, to determine the predisposition to the disease, etc. | 08-06-2009 |
| 20090197250 | METHODS AND NUCLEIC ACIDS FOR THE ANALYSIS OF GENE EXPRESSION ASSOCIATED WITH THE PROGNOSIS OF PROSTATE CELL PROLIFERATIVE DISORDERS - Particular aspects provide novel methods and compositions (e.g., nucleic acids, kits, etc.) having substantial utility for providing a prognosis of prostate cell proliferative disorders. In particular aspects, this is achieved by the analysis of the expression status of a panel of genes, or subsets thereof. | 08-06-2009 |
| 20090197251 | Process for marking products with nucleic acids for proving the identity and origin of the products - The invention relates generally to product identification and, more particularly, to processes for marking products with nucleic acids to establish the identity and origin of the products, to track them and to determine their quantity in the event of blending or dilution with other products. The invention also relates to the use of nucleic acids which code characters and character sequences for marking products and to products containing nucleic acids which code characters and character sequences. | 08-06-2009 |
| 20090197252 | Detection of Mutations - The present invention relates to a polypeptide which is flavin-containing monooxygenase 3 (FMO3), wherein said FMO3 is a polypeptide comprising at least a sequence having at least 85% identity with the polypeptide sequence SEQ ID NO: 15, in particular to a polypeptide which is a functionally altered mutant of flavin-containing mono oxygenase 3 (FMO3) leading to a buildup of trimethylamine in an animal. Further, the invention relates to nucleic acid sequences encoding said polypeptide, and to the complements of such nucleic acid sequences. Such nucleic acid sequences and fragments thereof may be useful e.g. as primers. The invention further relates to various methods for determining the presence in e.g. a nucleic acid sample of a nucleic acid sequence encoding a mutated FMO3 polypeptide. | 08-06-2009 |
| 20090197253 | HIGH FREQUENCY OF NEUREXIN 1BETA SIGNAL PEPTIDE STRUCTURAL VARIANTS IN PATIENTS WITH AUTISM - The three β-neurexins have similar roles in synaptogenesis and interact with the neuroligins. Mutations located within the gene encoding neurexin 1 have been identified as molecular markers associated with autism and autism-related disorders. The estimated attributable risk is 2%. The invention provides methods of diagnosing or predicting susceptibility to developing autism in an individual by determining the presence or absence of one or more genetic variant of a neurexin 1 gene in an individual. | 08-06-2009 |
| 20090197254 | VARIANT SCORPION PRIMERS FOR NUCLEIC ACID AMPLIFICATION AND DETECTION - Disclosed herein are methods of detecting target nucleic acids. In particular, methods for avoiding loss of the fluorescent label form an amplicon that is generated using a Scorpion primer and a polymerase with 5′ exonuclease activity. The methods use a Scorpion primer which comprises a fluorophore, a quencher, and in 5′ to 3′ order, a probe region, a linker region and a primer region, wherein the quencher is located at or near the 5′ end, and, wherein the primer is complementary to the target nucleic acid and the probe region hybridizes to a complementary sequence in an extension product of the primer. The methods provide for detection of target nucleic acids in simplex or multiplex formats. | 08-06-2009 |
| 20090197255 | Determining a predisposition to cancer - The present invention relates to methods and kits for determining a predisposition for developing cancer, e.g., prostate and/or breast cancer, due to a germline mutation of a NBS1 gene. The present invention also relates to surveillance protocols for developing cancer, e.g., prostate and/or breast cancer, due to germline mutation of a NBS1 gene. | 08-06-2009 |
| 20090197256 | Use of a fibroblast growth factor-binding protein for the treatment and diagnosis of diabetic wound healing problems - The present invention relates to the use of fibroblast growth factor-binding protein (FGF-BP) polypeptides, and functional variants of these polypeptides, respectively, or of nucleic acids encoding these polypeptides or variants, or of functional variants of these nucleic acids, and/or of a cell which is expressing an FGF-BP polypeptide or functional variants thereof, for treating, diagnosing and/or preventing wound healing disturbances which are characterized by a reduced quantity of FGF-BP, in particular diabetes-associated wounds, and also to the use of at least one FGF-BP polypeptide as depicted in SEQ ID No. 1 and SEQ ID No. 3 and/or of at least one FGF-BP-encoding nucleic acid as depicted in SEQ ID No. 2 and SEQ ID No. 4 and/or of antibodies or antibody fragments which are directed against an FGF-BP polypeptide which can be used in accordance with the invention, or functional variants thereof, and/or of a cell which is expressing an FGF-BP polypeptide or functional variants thereof, for identifying pharmacologically active substances which increase the activity of expression of FGF-BP. | 08-06-2009 |
| 20090197257 | PAIRED-END READS IN SEQUENCING BY SYNTHESIS - The disclosure provides methods of generating paired reads in sequencing-by-synthesis process, particularly, in systems with relatively short read lengths (e.g., 15-35 bases), such as for example, in single molecule sequencing by synthesis. Several implementations of the methods are provided. Of particular advantage are the methods that permit re-sequencing of the template, which yields lower error rates. The invention further provides methods of using paired reads, for example, for positioning them over repeats or for assembly into large sequences, including whole genome assembly. | 08-06-2009 |
| 20090197258 | PRIMARY RAT HEPATOCYTE TOXICITY MODELING - The present invention is based on the elucidation of the global changes in gene expression and the identification of toxicity markers in tissues or cells exposed to a known toxin. The genes may be used as toxicity markers in drug screening and toxicity assays. The invention includes a database of genes characterized by toxin-induced differential expression that is designed for use with microarrays and other solid-phase probes. | 08-06-2009 |
| 20090197259 | Gene signature for diagnosis and prognosis of breast cancer and ovarian cancer - A first embodiment is a breast cancer prognosticator comprising a detection mechanism consisting a 15-gene signature. In addition there are embodiments comprised of 23-gene signatures and 28-gene signatures. The 28-gene signature may also be used for the prognosis of ovarian cancer. A second embodiment is a method to determine metastatic potential, relapse potential, or both in breast cancer patients comprising collecting a sample from an individual, removing marker-derived polynucleotide from said sample, using a detection mechanism to search for positive matches of said polynucleotides and either the 15, 23, or 28-gene signatures, and developing a quantitative expression profile. Utilizing risk analysis the individual can be placed into one of two or more groups predicting risk and/or clincopathogic variables. Another embodiment is a method to determine relapse free potential in breast cancer patients comprising collecting a sample from an individual, removing marker-derived polynucleotide from said sample, using a detection mechanism to search for positive matches of said polynucleotides and a 24-gene signature, and developing a quantitative expression profile. | 08-06-2009 |
| 20090197260 | METHOD FOR PREDICTING THE VIABILITY AND VITALITY OF BACTERIA USABLE IN STRESSING ENVIRONMENT - A method for predicting viability, vitality and stability of bacteria, which are usable in stressing environments, produced in the form of bacterial preparations and packed in a frozen or lyophilised form, includes pre-suspending the bacterial preparations in non-stressing aqueous nutrient medium, carrying out a molecular measurement of at least one type of marker gene whose stress state is representative for each considered bacterium species, measuring intracellular pH and in comparing the measured values with reference and/or reference standard values. The use of the inventive method for controlling quality of stress adaptation of bacterial preparations and for selecting such preparations is also disclosed. The invention in particular relates to malolactic and probiotic ferments. | 08-06-2009 |
| 20090197261 | APTAMER- AND NUCLEIC ACID ENZYME-BASED SYSTEMS FOR SIMULTANEOUS DETECTION OF MULTIPLE ANALYTES - The present invention provides aptamer- and nucleic acid enzyme-based systems for simultaneously determining the presence and optionally the concentration of multiple analytes in a sample. Methods of utilizing the system and kits that include the sensor components are also provided. The system includes a first reactive polynucleotide that reacts to a first analyte; a second reactive polynucleotide that reacts to a second analyte; a third polynucleotide; a fourth polynucleotide; a first particle, coupled to the third polynucleotide; a second particle, coupled to the fourth polynucleotide; and at least one quencher, for quenching emissions of the first and second quantum dots, coupled to the first and second reactive polynucleotides. The first particle includes a quantum dot having a first emission wavelength. The second particle includes a second quantum dot having a second emission wavelength different from the first emission wavelength. The third polynucleotide and the fourth polynucleotide are different. | 08-06-2009 |
| 20090197262 | BORDETELLA DETECTION ASSAY - Disclosed herein are methods and compositions for detecting | 08-06-2009 |
| 20090197263 | Compare-MS: Method Rapid, Sensitive and Accurate Detection of DNA Methylation - The present invention provides methods and kits useful for enriching, identifying and quantifying methylated DNA3 particularly hypermethylated CpG islands by digesting a sample with a methylation-sensitive restriction endonuclease and capturing methylated restriction fragments with a methyl-binding capture reagent. The methods of the invention may be used in the detection of cancer, particularly detection of prostate cancer. | 08-06-2009 |
| 20090197264 | METHOD FOR PREDICTION OF SENSITIVITY TO 5-FLUOROURACIL-TYPE ANTICANCER AGENT - A factor affecting sensitivities to anticancer agents is analyzed and the validity of the factor is demonstrated. | 08-06-2009 |
| 20090197265 | EPIGENETIC ANALYSES - A method of carrying out epigenetic analysis on an analyte biological sample, the method comprising carrying out chromatin immunoprecipitation on an analyte biological sample, characterised in that the method comprises a step of contacting the analyte biological sample with carrier chromatin. The method of the invention wherein the analyte biological sample comprises less than one million cells. The method of the invention wherein the analyte biological sample comprises less than about 6 μg of DNA and/or less than about 12 μg of chromatin. | 08-06-2009 |
| 20090197266 | METHODS FOR OPTIMIZING CLINICAL RESPONSIVENESS TO METHOTREXATE THERAPY USING METABOLITE PROFILING AND PHARMACOGENETICS - The present invention provides methods for optimizing clinical responsiveness to chemotherapy in an individual through genotypic analysis of polymorphisms in at least one gene. The methods of the present invention may further comprise determining the level of at least one long-chain methotrexate polyglutamate (MTXPG) in a sample obtained from the individual. The present invention also provides methods for generating a pharmacogenetic index for predicting clinical responsiveness to chemotherapy in an individual through genotypic analysis of polymorphisms in at least one gene. In addition, the present invention provides methods for optimizing therapeutic efficacy of chemotherapy in an individual by calculating the level of at least one long-chain MTXPG in a sample obtained from the individual. | 08-06-2009 |
| 20090197267 | METHODS FOR OPTIMIZING CLINICAL RESPONSIVENESS TO METHOTREXATE THERAPY USING METABOLITE PROFILING AND PHARMACOGENETICS - The present invention provides methods for optimizing clinical responsiveness to chemotherapy in an individual through genotypic analysis of polymorphisms in at least one gene. The methods of the present invention may further comprise determining the level of at least one long-chain methotrexate polyglutamate (MTXPG) in a sample obtained from the individual. The present invention also provides methods for generating a pharmacogenetic index for predicting clinical responsiveness to chemotherapy in an individual through genotypic analysis of polymorphisms in at least one gene. In addition, the present invention provides methods for optimizing therapeutic efficacy of chemotherapy in an individual by calculating the level of at least one long-chain MTXPG in a sample obtained from the individual. | 08-06-2009 |
| 20090197268 | METHODS FOR OPTIMIZING CLINICAL RESPONSIVENESS TO METHOTREXATE THERAPY USING METABOLITE PROFILING AND PHARMACOGENETICS - The present invention provides methods for optimizing clinical responsiveness to chemotherapy in an individual through genotypic analysis of polymorphisms in at least one gene. The methods of the present invention may further comprise determining the level of at least one long-chain methotrexate polyglutamate (MTXPG) in a sample obtained from the individual. The present invention also provides methods for generating a pharmacogenetic index for predicting clinical responsiveness to chemotherapy in an individual through genotypic analysis of polymorphisms in at least one gene. In addition, the present invention provides methods for optimizing therapeutic efficacy of chemotherapy in an individual by calculating the level of at least one long-chain MTXPG in a sample obtained from the individual. | 08-06-2009 |
| 20090197269 | Transloca |
