Entries |
Document | Title | Date |
20080199851 | METHODS AND COMPOSITIONS FOR ANALYTE DETECTION - The present invention is directed to methods and apparatus for detection of one or more analytes. Analytes include agents or components of infectious agents such as pathogenic virus, as well as enzymes, proteins and biomarkers. | 08-21-2008 |
20080199852 | Detection method for human pappilomavirus (HPV) and its application in cervical cancer - Embodiments of the invention provide methods, assays, and kits for detecting HPV infection and HPV associated epithelial cell abnormalities, most notably those associated with pre-malignant and malignant epithelial cell lesions. Detection of HPV DNAs, genomes, and/or oncoproteins by nucleic acid hybridization assays and immunological assays can be used in early clinical screening for HPV infection and diagnosis for cervical cancer. The polypeptides, recombinant proteins, antibodies, nucleic acids, and various detection methods thereof are particularly useful for diagnosing carcinomas of the uterine cervix and those at risk of developing cervical cancer. | 08-21-2008 |
20080206740 | Sampling Method and Device - Disclosed herein is test device and method for detection of sample analytes in which after sampling has occurred a closure is provided. Such a test device and method can be usefully employed to detect a variety of analytes including microorganisms. | 08-28-2008 |
20080206741 | Virus Recovery Medium, Use Thereof and Viral Diagnostic Kit Including Same - The present invention relates to a virus recovery medium and a viral diagnostic kit comprising the same. The virus recovery medium is supplemented with a hormone and an enzyme. The hormone is preferably a glucocorticoid hormone, more preferably dexamethasone. The enzyme is preferably a protease, more preferably trypsin. | 08-28-2008 |
20080206742 | Inhibition of HIV-1 virion production by a transdominant mutant of integrase interactor 1 (INI1)/hSNF5 - Peptides comprising an Rpt1 domain of an INI1/hSNF5 which inhibit HIV-1 production in a human cell, and vectors encoding those peptides are provided. Also provided are methods of inhibiting HIV-1 production in a cell, or spread of the HIV-1 to another cell, by treating the cells with the above peptides or vectors. Other methods of inhibiting HIV-1 production in a cell, or spread of the HIV-1 to another cell, by inhibiting production of INI1/hSNF5 are provided. Additionally, methods of determining whether a test compound inhibits HIV-1 virion production in a mammalian cell, or spread of the HIV-1 to another cell, are provided. Those methods comprise determining whether the test compound inhibits the production of INI1/hSNF5 or disrupts the interaction of HIV-1 integrase with INI1/hSNF5. | 08-28-2008 |
20080213747 | FLUORESCENT POLYMER SUPERQUENCHING-BASED BIOASSAYS - A chemical composition including a fluorescent polymer and a receptor that is specific for both a target biological agent and a chemical moiety including (a) a recognition element, (b) a tethering element, and (c) a property-altering element is disclosed. Both the fluorescent polymer and the receptor are co-located on a support. When the chemical moiety is bound to the receptor, the property-altering element is sufficiently close to the fluorescent polymer to alter the fluorescence emitted by the polymer. When an analyte sample is introduced, the target biological agent, if present, binds to the receptor, thereby displacing the chemical moiety from the receptor, resulting in an increase of detected fluorescence. Assays for detecting the presence of a target biological agent are also disclosed. | 09-04-2008 |
20080213748 | Method for Detection and Analysis of Macromolecular Complexes - The present invention relates to a method for the detection and/or analysis of the structure of macromolecular complexes and/or macromolecules comprising the steps of (a) purifying or separating said macromolecular complexes and/or macromolecules from a sample containing said macromolecular complexes and/or macromolecules by applying in a porous matrix a separation force in a first dimension (X-axis); (b) transferring in a second dimension (Z-axis) by adsorption forces the macromolecular complexes and/or macromolecules purified or separated in step (a) from the porous matrix onto a carrier wherein said carrier contacts the surface of the porous matrix and is positioned parallel to the surface of said matrix and parallel to the direction of the separation force applied in step (a); (c) immobilizing the macromolecular complexes and/or macromolecules on said carrier after transfer of step (b); and (d) assessing the structure of the macromolecular complexes and/or macromolecules on said carrier after immobilization of step (c). | 09-04-2008 |
20080213749 | Compositions and methods for detecting human metapneumovirus - Disclosed are oligonucleotides useful in methods for determining whether a sample contains a human metapneumovirus or has an increased likelihood of containing a human metapneumovirus, a virus which is a causative agent of respiratory tract disease in humans. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the fusion protein of a human metapneumovirus, are useful as forward and reverse primers for a polymerase chain reaction using reverse transcripts of RNA from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains a human metapneumovirus or has an increased likelihood of containing a human metapneumovirus. | 09-04-2008 |
20080213750 | HCV Variants and Related Methods - HCV variants are described. The variants include polynucleotides comprising non-naturally occurring HCV sequences and HCV variants that have a transfection efficiency and ability to survive subpassage greater than HCV that have wild-type polyprotein coding regions. Expression vectors comprising the above polynucleotides and HCV variants are also described, as are the provision of cells and host cells comprising the expression vectors. Methods for identifying a cell line that is permissive for infection with HCV are also provided, as are vaccines comprising the above polynucleotides in a pharmaceutically acceptable carrier. Additionally, methods for inducing immunoprotection to HCV in a primate are described, as are methods for testing a compound for inhibiting HCV replication. | 09-04-2008 |
20080213752 | Bacteriophages as Selective Agents - Compositions containing bacteriophages and methods of using bacteriophages in microorganism detection assays and microbial growth and plating media are disclosed. The lytic ability of these phages to control the growth of non-target populations provides superior sensitivity and specificity to detection assays and reduces false negative and false positive results. The removal of contaminating bacteria reduces the microbial competition for nutrients in the growth media thereby increasing the efficiency and productivity of the culture. The phage treatment of the sample increases the proportion of target microorganisms in the sample over contaminating bacteria thereby requiring less time for enrichment to obtain a significant signal improving overall signal to noise ratio in assays and providing for higher yield of end product in microbiological production systems. | 09-04-2008 |
20080213753 | METHOD FOR THE VERIFICATION OF THE REMOVAL OF VIRUSES TO VALIDATE FILTERS AND FILTERING PROCESSES - Disclosed is a method for verification of the removal of viruses to validate filters, filtering processes, physical and chemical inactivation processes, or adsorptive removal processes in predefined process conditions that are simulated on a small scale. According to said method, viruses are cultured in suitable cell lines in a first step, a virus suspension is obtained after solubilizing cells in a second step, the virus suspension obtained is added in a third step to a protein solution that is to be analyzed, and the virus-containing protein solution is filtered through the filter that is to be validated, and the removal of viruses is then analyzed in a fourth step. The virus suspension is first processed via a membrane adsorber following step two, the viruses being bonded to the membrane adsorber and contaminants being removed with the aid of a detergent buffer solution, while the bonded, purified viruses are eluted from the membrane adsorber area and are added to the protein solution that is to be analyzed as a concentrated virus suspension in step three. | 09-04-2008 |
20080220408 | Protein Scaffolds and Viral Particles For Detecting Analytes - The present invention relates to compositions and methods for detecting analytes using detectably labeled fluorescent protein scaffolds. In certain embodiments of the invention, the scaffolds are viral particles in which the capsid viral structure provides a scaffold to attach detectably labeled fluorescent dyes and capture moieties that can be utilized to determine the presence of a desired analyte in a sample using any suitable method. The protein scaffold can contain amino acids carrying reactive groups (e.g., amines and thiols) that are spatially distributed on it with large enough separation to enable the attachment of a greater number of fluorescent label molecules without quenching | 09-11-2008 |
20080220409 | Antigen of Dengue Virus Type 1 - Antigens and B-cell epitopes derived from dengue virus type 1 are provided. The antigens are specifically immunoreactive with sera from individuals infected with dengue virus type 1 but not reactive with sera from healthy individuals and individuals infected with dengue virus type 2. The antigens and epitopes are useful for development of diagnostic kits and reagents, and are useful tools as well in determining whether an individual is infected with dengue virus type 1, and for distinguishing infection from dengue virus type 2. | 09-11-2008 |
20080220410 | METHOD FOR REPLICATING THE HEPATITIS C VIRUS - The invention concerns the use of cells capable of carrying out a process of prenylation of proteins coded by the hepatitis C virus (HCV) genome, such as prenylation of the NS5A protein, for replicating and, if required, the production of HCV or derivative viable mutants, in a suitable culture medium. | 09-11-2008 |
20080220411 | NON-LINEAR ROTATION RATES OF REMOTELY DRIVEN PARTICLES AND USES THEREOF - The present invention relates to biological sensors. In particular, the present invention relates to the use of remotely driven nonlinear rotation of particles (e.g., magnetic particles) for detection of cells such as microorganisms (e.g., bacteria and viruses). The present invention further relates to the use of remotely driven nonlinear rotation of particles for measurement of physical properties of a solution (e.g., viscosity). | 09-11-2008 |
20080220412 | Method and Means for Determining the Replication Rate of a Viral Population - The present invention relates to methods and means for determining the replication rate of a viral population. More specifically, the invention provides methods and means for determining the replication rate of a viral population by performing a linear regression on signal data generated by cells infected with dilutions of the viral population. The methods are useful for monitoring the progression of diseases associated with viruses, identifying effective drug regimens for the treatment of viral infections, and identifying and determining the biological effectiveness of potential therapeutic compounds. | 09-11-2008 |
20080227084 | ANALYSIS OF HIV-1 CORECEPTOR USE IN THE CLINICAL CARE OF HIV-1-INFECTED PATIENTS - A change in viral tropism occurs in many HIV positive individuals over time and can be indicated by a shift in coreceptor use from CCR5 to CXCR4. The shift in coreceptor use to CXCR4 has been shown to correlate with increased disease progression. In patients undergoing HAART, the predominant populations of virus can be shifted back to CCR5-mediated entry after the CXCR4-specific strains have emerged. The present invention relates to a diagnostic method to monitor coreceptor use in the treatment of human immunodeficiency virus (HIV) infection. The present invention further relates to a diagnostic method applied to HIV-positive individuals undergoing HAART to monitor the suppression of CXCR4 specific strains. The diagnostic methods can be used to assist in selecting antiretroviral therapy and to improve predictions of disease prognosis over time. | 09-18-2008 |
20080233557 | PNA Probes, Kits, and Methods for Detecting Lamivudine-Resistant Hepatitis B Viruses - Disclosed are peptide nucleic acid (PNA) probes to detect lamivudine resistant mutants of hepatitis B virus (HBV), which causes acute and chronic hepatitis, kits for detecting lamivudine resistant HBV comprising the PNA probes, and methods for detecting lamivudine resistant HBV by using the kits. They can accurately detect mutations of rtL180M, rtM204V, rtM204I and rtV2071 within B and C domains of HBV DNA polymerase gene, the main cause of lamivudine resistance, as well as mixed mutants of more than one mutant. They can detect lamivudine resistant HBV with high specificity and sensitivity. | 09-25-2008 |
20080233558 | Inhibitors of viral entry screening method - In one aspect the invention relates to a method for identifying inhibitors or viral entry comprising providing an indicator cell wherein said cell expresses a reporter gene and wherein said cell is capable of supporting entry by an effector particle, providing a candidate inhibitor of viral entry, co-compartmentalizing said candidate inhibitor and said indicator cell, contacting said indicator cell with an effector particle, incubating to allow any effector particle entry to take place, and assaying said indicator cell for reporter gene activity, wherein detection of reporter gene activity identifies the candidate inhibitor as an inhibitor for viral entry. Preferably the effector particle is HIV, preferably the reporter gene is a CD | 09-25-2008 |
20080233559 | Inhibition of HIV-1 Replication by Disruption of the Processing of the Viral Capsid-Spacer Peptide 1 Protein - Inhibition of HIV-1 replication by disrupting the processing of the viral Gag capsid (CA) protein (p24) from the CA-spacer peptide 1 (SP1) protein precursor (p25) is disclosed. Amino acid sequences containing a mutation in the Gag p25 protein, with the mutation resulting in a decrease in the inhibition of processing of p25 to p24 by dimethylsuccinyl betulinic acid or dimethylsuccinyl betulin, polynucleotides encoding such mutated sequences and antibodies that selectively bind such mutated sequences are also included. Methods of inhibiting, inhibitory compounds and methods of discovering inhibitory compounds that target proteolytic processing of the HIV Gag protein are included. In one embodiment, such compounds inhibit the interaction of the HIV protease enzyme with Gag by binding to Gag rather than to the protease enzyme. In another embodiment, viruses or recombinant proteins that contain mutations in the region of the Gag proteolytic cleavage site can be used in screening assays to identify compounds that target proteolytic processing. | 09-25-2008 |
20080233560 | DNA-transfection system for the generation of infectious influenza virus - The present invention is based on the development of a dual promoter system (preferably a RNA pol I-pol II system) for the efficient intracellular synthesis of viral RNA. The resultant minimal plasmid-based system may be used to synthesize any RNA virus, preferably viruses with a negative single stranded RNA genome. The viral product of the system is produced when the plasmids of the system are introduced into a suitable host cell. One application of the system is production of attenuated, reassortant influenza viruses for use as antigens in vaccines. The reassortant viruses generated by cotransfection of plasmids may comprise genes encoding the surface glycoproteins hemagglutinin and neuraminidase from an influenza virus currently infecting the population and the internal genes from an attenuated influenza virus. An advantageous property of the present invention is its versatility; the system may be quickly and easily adapted to synthesize an attenuated version of any RNA virus. Attenuated or inactivated RNA viruses produced by the present invention may be administered to a patient in need of vaccination by any of several routes including intranasally or intramuscularly. | 09-25-2008 |
20080233561 | Method for Measuring Cytopathic Effect Due to Viral Infection in Cells Using Electric Cell-Substrate Impedance Sensing - A method of measuring cytopathic effect in cells includes providing cells in culture, using electric cell-substrate impedance sensing (ECIS) to measure the resistance of current associated with the cells, and quantifying the cytopathic effect (CPE) associated with the cells based on the measured resistance. The cells may be identified as being infected with a virus if the CPE associated with the cells is above a predetermined level. Alternatively, the cells may be provided in a healthy monolayer and infected with a virus in order to measure the effect of the virus on CPE associated with the cells. Cells may also be treated with candidate antiviral agents and the effects of the agents on the virus-infected cells may be measured to screen for and identify actual antiviral agents. | 09-25-2008 |
20080241818 | Method and microarray for detecting herpesviruses - The present invention relates to a method and to a microarray for detecting herpesviruses. The invention provides new primers and oligonucleotides for detecting herpesviruses, in particular herpesviruses selected from the group comprising HSV-1, HSV-2, CMV, EBV, VZV, HHV-6A, HHV-6B and HHV-7. By using the method of the invention several different herpesviruses can be detected simultaneously from the same biological sample. | 10-02-2008 |
20080241819 | METHOD AND APPARATUS FOR ENHANCED BACTERIOPHAGE-BASED DIAGNOSTIC ASSAYS BY SELECTIVE INHIBITION OF POTENTIAL CROSS-REACTIVE ORGANISMS - A sample to be tested for the presence of a target microorganism is exposed to bacteriophage and conditions are provided to inhibit phage attachment to or replication in a potentially cross-reactive, non-target microorganism. The sample is incubated and assayed to detect the presence or absence of a bacteriophage marker to determine the presence or absence of the target microorganism. The inhibiting may comprise the addition of an inhibiting substance or the use of an inhibiting process. It may include inhibiting the growth of potentially cross-reactive bacteria while allowing growth of the target bacteria, selectively removing or blocking potential cross-reactive bacteria using selective binding agents or selectively destroying potentially cross-reactive bacteria. | 10-02-2008 |
20080241820 | MULTIPLEX CELLULAR ASSAYS USING DETECTABLE CELL BARCODES - We describe herein a cell-based multiplexing technique called detectable cell barcoding (DCB). In DCB, each individual sample is labeled with a different DCB signature that distinguishes each sample by one or both of detected intensity or type of detection characteristic. The samples are then combined and analyzed for a detectable characteristic of interest (e.g., presence of an analyte). By employing multiple distinct DCB labels at varying concentrations, one can perform multiplex analyses on up to hundreds or thousands (or more) of cell samples in a single reaction tube. DCB reduces reagent consumption by factors of 100-fold or more, significantly reduces data acquisition times and allows for stringent control sample analysis. | 10-02-2008 |
20080241821 | ASSAY FOR PORCINE CIRCOVIRUS PRODUCTION - The present invention provides methods for the determination of the viral titer of a culture of host animal host cells infected with a circovirus. The FACS-based methods of the invention may include determining the viability of the host cells in a cell culture medium supernatant and of those cells that remain adherent to a solid support. Detecting and measuring the percentage of cells that expressed the viral antigens ORF1 and ORF2 may determine the viral load of the cultured host cells. The yield of the virus may be established by the detection and measurement of both antigens in supernatant cells, for example 5 to 7 days from when the host cells are transferred to a serum free medium. The methods of the invention may yield rapid quantitative data. This allows the repeated in-process monitoring of the viral production throughout the incubation period, and ready selection of the most appropriate harvesting point. | 10-02-2008 |
20080248459 | Multiplex detection of agricultural pathogens - Described are kits and methods useful for detection of seven agricultural pathogens (BPSV; BHV; BVD; FMDV; BTV; SVD; and VESV) in a sample. Genomic sequence information from 7 agricultural pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample. | 10-09-2008 |
20080248460 | Composition and method for modulating an inflammatory response - The invention relates to compositions and methods comprising lymphotoxin-beta receptor (LTβR) modulators, which activate or inhibit LTβR signaling. LTβR modulators are useful for treating lymphocyte mediated immunological diseases and cancer, and more particularly, for regulating mitochondrial-mediated apoptosis. This invention relates to soluble forms of the LTβR complex proteins that act as LTβR activating or inhibiting agents. This invention also relates to the use of soluble molecules, directed against either the LTβR, its ligands, LIGHT and LTβ1α2, or its intracellular binding partners, that function to regulate LTβR signaling. A novel screening method for selecting soluble receptors, antibodies and other agents that modulate LTβR signaling is provided. | 10-09-2008 |
20080248461 | PNA Probes, Kits, and Methods for Detecting Genotypes of Human Papillomavirus - Disclosed are PNA probes capable of genotype specifically binding with Human Paillomavirus (HPV) DNA, kits for detecting HPV genotypes comprising the probes, and methods for detecting HPV genotypes by using the kits, which enables the accurate detection of all 24 genotypes of HPV found in cervix, diagnosis of combined infection with more than one HPV genotype, and detection of HPV genotypes with high specificity and sensitivity. | 10-09-2008 |
20080254440 | Anti-Sars Virus Antibody, Hybridoma Producing the Antibody and Immunoassay Reagent Using the Antibody - A monoclonal antibody which specifically recognizes SARS virus is provided, and an immunoassay, immunoassay reagent and immunoassay device for detecting the SARS virus using the monoclonal antibody are disclosed. The monoclonal antibody according to the present invention is a monoclonal antibody against a nucleoprotein of a corona virus causing severe acute respiratory syndrome (SARS). | 10-16-2008 |
20080254441 | Lateral-Flow Test Device Providing Improved Test Result Validity - The present invention refers to a lateral-flow test device providing improved test result validity comprising at least one indication means indicating whether a test result will be inconclusive and/or at least one indication means indicating that a test result is ready for read. It is further referred to a use of such lateral-flow test device and to a procedure for its manufacture. | 10-16-2008 |
20080254442 | Crystal structures of human peptide deformylase - The present invention provides the three-dimensional structure of human mitochondrial | 10-16-2008 |
20080254443 | Norovirus detection, methods and compositions therefor - A norovirus-permissive cell culture infected with a norovirus, and methods of culturing a norovirus, are disclosed. Norovirus-permissive cells include dendritic cell-lineage cells, and macrophage-lineage cells, such as dendritic cells, and macrophages having a deficiency in a cellular anti-viral pathway such as a STAT-1-dependent pathway, an interferon receptor-dependent pathway, or a PKR-dependent pathway. Also disclosed are methods of screening anti-viral compounds against norovirus-permissive cells infected with norovirus, and norovirus adapted to grow in fibroblasts as well as macrophages that are not deficient in a cellular anti-viral pathway. Methods of making a norovirus vaccine are also disclosed. A replicative form of norovirus as well as its use in the development of an anti-viral agent and a polypeptide expression system are also described. Further disclosed are methods of detecting norovirus in a sample. | 10-16-2008 |
20080254444 | Immunoassay Devices and Use Thereof - An immunoassay device has a housing defining a first opening for receiving a solution and a second opening through which a liquid sample is deposited, a strip of sorbent material having a test site with immobilized antigen or antibody for the ligand to be tested, and a filter for filtering the sample. The filter is located at the second opening and directly above the test site. The sorbent material defines a horizontal flow path in the housing for the solution from the first opening to the test site. In use, the sample is first applied via the filter to the test site, and then, after the ligand has been captured, a buffer added through the first opening is used to cause a secondary specific binder conjugated to a marker to migrate horizontally by capillary action to the test site where it can bind to the already captured ligand. This immunoassay offer several advantages over the traditional chromatographic immunoassays. | 10-16-2008 |
20080261197 | Nucleotide sequences of HIV-1 group (or subgroup) O retroviral antigens - An HIV-1 type (or subtype) O retrovirus protein, or a natural or synthetic polypeptide or peptide including at least a part of said protein, which is capable of being recognised by antibodies isolated from a serum resulting from infection by an HIV-1 type O VAU strain or an HIV-1 type (or subtype) O DUR strain. | 10-23-2008 |
20080261198 | Diagnostic Primers and Method for Detecting Avian Influenza Virus Subtype H5 and H5n1 - The present invention provides primers directed to conserved regions of the HA and NA genes of avian influenza virus subtypes H5 or H5N1, and provides a method for detecting avian influenza subtype H5 or H5N1. | 10-23-2008 |
20080261199 | RAPID DETECTION PROCESSES AND RELATED COMPOSITIONS - An improved detection method is described for an antigen such as a chemical compound, a peptide, or a nucleic acid. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional Western blot, Dot blot, ELISA and Immunohistology methods. | 10-23-2008 |
20080261200 | Detection of Nucleic Acid Mutations - A method for detecting a mutation in a target nucleic acid sequence in a sample, the target nucleic acid sequence comprising a first DNA strand and optionally the complementary strand thereof, said method comprising: (a) adding a detection primer to the nucleic acid, wherein the detection primer binds to the first DNA strand at a DNA sequence that comprises the mutation site; (b) extending the detection primer to form second DNA strands that are complementary to the first DNA strand; (c) adding an amplification primer to the nucleic acid, wherein the amplification primer binds to the second DNA strand and/or to the complementary strand, at a position away from the mutation site; (d) extending the amplification primer to form third DNA strands that are complementary to the second DNA strands, and/or additional copies of the first DNA strand; (e) annealing the DNA strands by complementary base pairing, to form nucleic acid duplexes, wherein if the two strands of the duplex have a mismatched residue at the mutation site, the duplex is a heteroduplex, and. wherein if the two strands of the duplex do not have a mismatched residue at the mutation site, the duplex is a homoduplex; and (d) detecting the presence of heteroduplexes and/or homoduplexes. | 10-23-2008 |
20080261201 | Methods and compounds to alter virus infection - The invention provides a method to identify an agent that alters parvovirus transduction of mammalian cells. Also provided is a method to enhance transgene expression in a mammalian cell, as well as a method to identify an agent that alters NADPH oxidase activity in parvovirus transduced mammalian cells. | 10-23-2008 |
20080261202 | Tagged Polyfunctional Reagents Capable of Reversibly Binding Target Substances in a pH-dependent Manner - Polyfunctional reagents are disclosed that are capable of reversibly binding to target substances, for example nucleic acid, proteins, polypeptides, cells, cell components, microorganisms or viruses, for use in purifying or otherwise manipulating them. The reagents comprise a tagging group for manipulating and/or detecting the target substance when bound to the polyfunctional reagent. The polyfunctional reagents work by binding the target substance at a first pH and then releasing it at a second pH, usually higher than the first. Examples of tagging groups include tagging group members of a specific binding pair which is capable of binding to a specific binding partner and/or a label. | 10-23-2008 |
20080261203 | Detection of Epstein-Barr Virus - The invention provides methods to detect EBV in biological samples using real-time PCR. Primers and probes for the detection of EBV are provided by the invention. | 10-23-2008 |
20080268423 | Compositions and Methods Related to Flavivirus Envelope Protein Domain III Antigens - The present invention concerns methods and compositions involving flavivirus envelope protein domain III antigens for the detection of virus and detection of antibodies against the virus. Such methods and compositions may be used to detect TBE serocomplex viruses or West Nile virus infection in a subject, patient, animal or biological fluid. The present invention also concerns kits for implementing such methods. In some embodiments, kits contain a recombinant TBE serocomplex virus or West Nile virus envelope protein domain III antigen. | 10-30-2008 |
20080268424 | Glut-1 as a Receptor for Htlv Envelopes and its Uses - The invention relates to the use of the ubiquitous vertebrate glucose transporter GLUT1, or of fragments or sequences derived thereof, for the in vitro diagnosis of cancers, when used as a tumor marker, or for the screening of compounds useful for the preparation of drugs for the prevention or the treatment of pathologies linked to an infection of an individual with a PTLV, or pathologies linked to an overexpression of GLUT1 on cell surfaces, or the in vitro detection of GLUT1 on cell surfaces. The invention also relates to pharmaceutical compositions containing GLUT1, or fragments or sequences derived thereof, and to their uses such as in the frame of the prevention or the treatment of pathologies linked to an infection of an individual with a PTLV. | 10-30-2008 |
20080268425 | METHODS AND KITS FOR DETECTING CLASSICAL SWINE FEVER VIRUS - The present invention provides methods and kits for detecting CSFV. The present invention also provides oligonucleotides for detecting CSFV. | 10-30-2008 |
20080268426 | IDENTIFYING VIRALLY INFECTED AND VACCINATED ORGANISMS - This document provides methods and materials related to assessing organisms for the presence or absence of anti-virus antibodies. For example, this document provides methods and materials that can be used to determine whether or not an organism (e.g., a member of a swine species such as a pig) contains anti-PRRS virus antibodies. In other embodiments, this document provides methods and materials that can be used to determine if a particular organism received a vaccine version of a virus, was infected with a naturally-occurring version of the virus, or is naïve with respect to the virus. | 10-30-2008 |
20080268427 | DETECTION OF BOVINE VIRAL DIARRHEA VIRUS IN HAIR SAMPLES - The present invention relates to a method of detecting whether a target animal is Bovine Viral Diarrhea Virus (BVDV) positive or negative by determining whether a gp48 protein-specific reagent binds to a gp48 Bovine Viral Diarrhea Virus protein or protein fragment, which retains antigenic specificity, from a target animal's hair sample. | 10-30-2008 |
20080274449 | ASSAY FOR DETECTION OF HUMAN PARVOVIRUS B19 NUCLEIC ACID - Nucleic acid oligomers specific for human parvovirus B19 genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus B19 nucleic acid in biological specimens is disclosed. Compositions for detecting the presence of parvovirus B19 genomic DNA in human biological specimens are disclosed. | 11-06-2008 |
20080274450 | Methods and applications of molecular beacon imaging for identifying and validating genomic targets, and for drug screening - A method for characterizing the gene expressions of a sample of cells of a living subject, where the sample of cells is characterized by one or more marker sequences. In one embodiment, the method includes the steps of providing one or more types of molecular beacons, each type of molecular beacons designed to have a corresponding probe sequence complementary to one of the one or more marker sequences and an emitter capable of emitting photons of a unique color such that when one of the type of molecular beacons targets the one of the one or more marker sequences the sample of cells, the emitter of the molecular beacon emits photons of the unique color, thereby generating a photon signal of the unique color; treating the sample of cells with the one or more types of molecular beacons; and detecting photon signals of one or more colors of the sample of cells so as to characterizing the gene expressions of the sample of cells, wherein the one or more types of molecular beacons are designed such that the photon signals of the one or more colors are detectable without a need of signal amplification. | 11-06-2008 |
20080274451 | Body for flow-through cells and the use thereof - A system of flow-through cells is obtained by assembling a body with a base plate. The body has a first spring element and a first stop, disposed on opposite end sections of the body, at least one second spring element and a corresponding second stop, disposed on opposite faces of the body, and at least one support element and at least one retaining element, which are adapted for the exact positioning of the body in the receiving openings of a support in three directions. | 11-06-2008 |
20080280283 | Methods For Assessing Fatigue Level and Applications Thereof - Level of fatigue that accompanies everyday life or a disease can be simply, easily, and quantitatively assessed by obtaining a body fluid from a test subject and measuring the amount of human herpesvirus in the body fluid. Furthermore, the anti-fatigue potency of anti-fatigue substances and anti-fatigue food products can be measured. | 11-13-2008 |
20080280284 | Nucleic Acid Sequences That Can Be Used as Primers and Probes in the Amplification and Detection of Hsv Dna and Method for the Amplification and Detection of Hsv Dna Using a Transcription Based Amplification - The present invention is related to a pair of oligonucleotide primers for the amplification of HSV nucleic acid comprising: a) an oligonucleotide, 10-50 nucleotides in length, preferably 10-35 nucleotides in length, comprising at least a fragment of 10 nucleotides of a sequence selected from the group consisting of: 5′-ACGTTCACCAAGCTGCTGCT-3′, or its complementary sequence and b) an oligonucleotide, 10-50 nucleotides in length, preferably 10-35 nucleotides in length, comprising at least a fragment of 10 nucleotides of a sequence selected from the group consisting of: 5′CCAGGGCCCTGGAGGTGCGG-3′, or its complementary sequence. The invention also relates to probes, method for amplifying an HSV DNA target, method of specific ou aspecific detection of HSV type 1 and 2 and test kit to do possible the detection of HSV. The present invention is especially useful in methods for practicing nucleic acid test. | 11-13-2008 |
20080280285 | Systems and Methods For Testing using Microfluidic Chips - Disclosed are methods, devices and systems for biological and chemical sample processing using microfluidic chips. The disclosed microfluidic chips contain at least two detection zones for interacting with pre-selected RNA sequences, DNA sequences, antibodies, or antigens to determine their presence in the sample. Systems are also described comprising a cassette having at least one port and a sample inlet in fluid communication with a detection zone for interacting with pre-selected RNA sequences, DNA sequences, antibodies, or antigens, or mixtures thereof, if present, in a sample. Methods for concurrent testing of at least two of RNA, DNA, antibody, and antigen in a sample are also described, as are methods for testing for pre-selected pathogens and microfluidic methods. | 11-13-2008 |
20080286751 | Dispensing Device For Microfluidic Droplets Especially For Cytometry - The invention relates to a dispensing device for droplets comprising a first channel ( | 11-20-2008 |
20080286752 | Methods for the production of HCV, assaying HCV entry, and screening drugs and cellular receptors for HCV - The invention provides cell culture methods that efficiently produce new infectious HCV virions where such methods are based on the unexpected finding that culturing cells at lower temperatures, i.e., from about 20° C. to about 34° C., enables efficient methods dependent upon HCV E1E2 mediated fusion. The invention also provides fusion assay methods that are robust and reliable because of, at least in part, specific pH conditions, and HCV E1 and E2 proteins that contain a dimerization domain. The present methods are useful for propagating infectious HCV, for improved diagnostics, drug screening and basic research efforts relating to HCV receptor binding, HCV entry (binding (attachment) and fusion), replication, virion assembly and release. In another respect, the present invention provides methods for detecting HCV E1E2 mediated fusion, and related methods for identifying drugs or other molecules that can inhibit HCV fusion and for identifying mutations that can inhibit HCV fusion. | 11-20-2008 |
20080286753 | Wicking Cassette Method and Apparatus for Automated Rapid Immunohistochemistry - A sample processing system that may be configured to achieve automatic withdrawal of a substance at the end of appropriate processing sequences such as histochemical processing may involve a plurality of samples for which substances removed by moving a wicking cartridge that may have sequentially advanced absorbsant material on rolls that are advanced an appropriate amount based upon usage in sequences such as repeated elimination and reapplication of a fluidic substance perhaps through the action of capillary motion in order to refresh a microenvironment adjacent to a sample such as a biopsy or other such sample. Snap in wicking cassettes and perhaps antibody and other substances may be included to ease operator actions and to permit location specific substance applications perhaps by including single container multiple chamber multiple fluidic substance magazines, linearly disposed multiple substance source, and primary antibody cartridges. | 11-20-2008 |
20080286754 | Mutational profiles in HIV-1 reverse transcriptase correlated with phenotypic drug resistance - The invention provides novel mutations, mutation combinations or mutational profiles of HIV-1 reverse transcriptase and/or protease genes correlated with phenotypic resistance to HIV drugs. More particularly, the present invention relates to the use of genotypic characterization of a target population of HIV and the subsequent correlation of this information to phenotypic interpretation in order to correlate virus mutational profiles with drug resistance. The invention also relates to methods of utilizing the mutational profiles of the invention in databases, drug development, i.e., drug design, and drug modification, therapy and treatment design, clinical management and diagnostic analysis. | 11-20-2008 |
20080286755 | Immunoglobin IgG3 as a marker for protecting against infectious viral diseases, and the uses of the same - The invention relates to a novel variant of isolated and/or purified immunoglobulin IgG3 which can be used as a marker for protecting against infectious viral diseases such as AIDS, as a diagnostic tool, or as a preventative and curative medicament. The invention also relates to corresponding in vitro diagnostic methods. | 11-20-2008 |
20080286756 | COMPOSITIONS AND METHODS FOR DIAGNOSING AND TREATING SEVERE ACUTE RESPIRATORY SYNDROME (SARS) - The present invention relates to the fields of immunochemistry and pharmacology. Methods and compositions are described for the diagnosis and treatment of SARS CoV infection. More specifically, the application discloses nucleic acids and peptides of the spike glycoprotein of SARS CoV that provide prognostic and therapeutic compositions in treatment of individuals contracting, or in danger of contracting SARS CoV. The peptides of the invention are also useful in producing antibodies against the SARS CoV glycoprotein. | 11-20-2008 |
20080286757 | Method and Apparatus for Identification of Microorganisms Using Bacteriophage - A sample is tested for the presence of bacteria, such as in an automatic blood culturing apparatus. If bacteria are determined to be present, a bacteriophage-based bacteria identification process is performed to identify the bacteria present. A plurality of bacteria detection processes, such as a blood culture test and Gram stain test may be carried out prior to the bacteria identification process. A bacteriophage-based antibiotic resistance test or antibiotic susceptibility test is also conducted on the sample. | 11-20-2008 |
20080286758 | REAGENTS AND KITS FOR DETECTION OF INFLUENZA VIRUS AND THE LIKE - The present invention relates to reagents and methods for influenza virus detection. These reagents and methods disclosed in the present invention enable simple, rapid, specific and sensitive detection of influenza virus types A and B. These reagents are N-acetylneuraminic acid-firefly luciferin conjugates which can be cleaved by influenza virus neuraminidase. | 11-20-2008 |
20080286759 | Hepatocyte Bioreactor System For Long Term Culture of Functional Hepatocyte Spheroids - A rotating wall vessel is used as a culture vessel and bioreactor for the cultivation of hepatocytes in the form of spheroids to generate a culture with many properties of the intact liver. These properties include enzyme activity comparable to fresh cells and long-term maintenance of viability and cellular function for periods on the order of months. The cultures may be used to produce hepatocyte products, evaluate metabolism of an agent, propagate Hepatitis C virus and test agents as inhibitors of this virus. Thus, the culture system disclosed herein makes long term functional cultivation of human hepatocytes feasible. | 11-20-2008 |
20080293036 | Monoclonal Antibodies to Hiv-1 and Methods of Using Same - The present invention provides monoclonal antibodies to HIV-1 Vpr and hybridoma cell lines that produce the monoclonal antibodies to HIV-1 Vpr. Methods for use of such antibodies in the detection of HIV-1 infection are also provided. | 11-27-2008 |
20080293037 | Sequences Diagnostic For Shrimp Pathogens - Primers have been isolated that are diagnostic for the detection of the infectious hypodermal and hematopoietic necorsis virus (IHHNV). The primers are based on a new portion of the IHHNV genome and may be used in primer directed amplification or nucleic acid hybridization assay methods. | 11-27-2008 |
20080293038 | Method for Determining Resistance of Hiv to Nucleoside Reverse Transcriptase Inhibitor Treatment - The present invention provides methods and devices for predicting whether an HIV-1 is resistant to an antiviral drug based on the HIV-1's genotype. In one aspect, the invention provides methods comprising determining whether a mutation or combination of mutations associated with NRTI resistance are present, as disclosed herein, thereby assessing the effectiveness of FTC therapy in the HIV-infected subject. Computer implemented methods comprising determining HIV-1 resistance are provided. | 11-27-2008 |
20080293039 | VP7 gene of human rotavirus and composition for diagnosis of human rotavirus infection comprising primer or probe specific to thereof - The present invention relates to a VP7 gene of human rotavirus and a composition for diagnosis of human rotavirus infection comprising primer or probe specific to thereof, and more particularly to a VP7 gene encoding the amino acid sequence set forth in SEQ ID NO:2 and a composition for diagnosis of human rotavirus infection comprising primer or probe specific to thereof. The human rotavirus VP7 gene according to the present invention will be useful for diagnosis of novel G11 type human rotavirus infection, and will be used for development of rotavirus vaccine. | 11-27-2008 |
20080293040 | INFLUENZA B VIRUSES WITH REDUCED SENSITIVITY TO NEURAMINIDASE INHIBITORS - An isolated influenza B virus which has reduced sensitivity to one or more neuraminidase (NA) inhibitors, wherein the reduced sensitivity to one or more NA inhibitors is associated with a residue in NA other than Ile at position 222, a residue in NA other than Ser at a position 250, or a residue in NA other than Gly at position 402, as well as methods to detect such a virus or determine agents that inhibit the infection or replication of such as virus, are provided. | 11-27-2008 |
20080293041 | Methods and Devices for Detection of the Strain of a Pathogen - Provided are methods and devices for determining the strain of a pathogen in a sample. | 11-27-2008 |
20080293042 | Detection of Contamination of Municipal Water Distribution Systems - A system for the detection of contaminates of a fluid in a conduit. The conduit is part of a fluid distribution system. A chemical or biological sensor array is connected to the conduit. The sensor array produces an acoustic signal burst in the fluid upon detection of contaminates in the fluid. A supervisory control system connected to the fluid and operatively connected to the fluid distribution system signals the fluid distribution system upon detection of contaminates in the fluid. | 11-27-2008 |
20080299544 | Hcv Multiple Epitope Fusion Antigens With Modified Proteolytic Cleavage Sites And Uses Thereof - Modified HCV multiple epitope fusion antigens (MEFAs) are described. The proteins include modified sequences such that proteolytic cleavage of the MEFAs by HCV NS3 protease is inhibited. HCV immunoassays including the modified MEFAs are also described. | 12-04-2008 |
20080299545 | CHROMATOGRAPHIC METHODS FOR ASSESSING ADENOVIRUS PURITY - Methods for determining the quantity, quality and purity of a previously purified virus sample are disclosed. Such methods, which include the use of high performance size exclusion chromatography to determine these attributes are also disclosed. | 12-04-2008 |
20080311556 | Sense Antiviral Compound and Method for Treating Ssrna Viral Infection - The invention provides sense antiviral compounds and methods of their use in inhibition of growth of viruses of the Flaviviridae, Picornoviridae, Caliciviridae, Togaviridae, Coronaviridae families and hepatitis E virus in the treatment of a viral infection. The sense antiviral compounds are substantially uncharged morpholino oligonucleotides having a sequence of (12-40) subunits, including at least (12) subunits having a targeting sequence that is complementary to a region associated with stem-loop secondary structure within the 3′-terminal end (40) bases of the negative-sense RNA strand of the virus. | 12-18-2008 |
20080311557 | DEVICE, KIT AND METHOD FOR HOOKWORM ANTIGEN CAPTURE AND DETECTION - A device, kit and method for detecting the presence or absence of hookworm antigens. The device, kit and method of the present invention may be used to confirm the presence or absence of hookworm in a fecal sample that may be infected with one or more of roundworm, whipworm, tapeworm and heartworm, and whether or not hookworm ova are present in the sample. Further, the device, kit and method of the present invention may be used to confirm the presence or absence of hookworm in a fecal sample excreted by a canine animal as early as nine days after the animal first becomes infected with hookworm. | 12-18-2008 |
20080311558 | Methods For Rapid Identification Of Pathogens In Humans And Animals - The present invention provides methods of: identifying pathogens in biological samples from humans and animals, resolving a plurality of etiologic agents present in samples obtained from humans and animals, determining detailed genetic information about such pathogens or etiologic agents, and rapid detection and identification of bioagents from environmental, clinical or other samples. | 12-18-2008 |
20080311559 | DETECTION OF NUCLEIC ACIDS FROM MULTIPLE TYPES OF HUMAN PAPILLOMAVIRUSES - Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed. | 12-18-2008 |
20080311560 | Oligonucleotides For Detecting Human Papilloma Virus In A Test Sample - Oligonucleotides targeted to HPV Type 16 and/or Type 18 nucleic acid sequences which are particularly useful to aid in detecting HPV type 16 and or 18 are described. The oligonucleotides can aid in detecting HPV Type 16 and/or Type 18 in different ways such as by acting as hybridization assay probes, helper probes, and/or amplification primers. | 12-18-2008 |
20080311561 | Kits and Method For Detecting Human Papilloma Virus With Oligo Nucleotide Bead Array - Provided are determining methods of human papillomavirus (HPV) genotypes with a high sensitivity. The method includes performing two-step PCRs on an HPV L1 gene in a sample to be analyzed as a biotin-labeled, single-stranded L1 gene, performing a hybridization reaction on the biotin-labeled, single-stranded L1 gene with a HPV genotype detection probe, reacting the hybridization reaction product with fluorescent substance combined with streptavidine, and measuring a fluorescent substance level to identify the HPV genotype. The detection method has high sensitivity enough to detect an extremely small amount of HPV in the sample. In addition, the high specificity exhibited by the detection method enables accurate diagnosis specific to HPV type. | 12-18-2008 |
20080318204 | Highly-Sensitive Genomic Assays Employing Chimeric Bacteriophage Standards - Methods are provided for sensitively quantitating at least one pre-selected DNA sequence in a biological sample utilizing hybridization methodology, the method employing as an internal standard an infectious bacteriophage particle comprising a detectable target DNA sequence other than that present in the pre-selected DNA sequence or in DNA quantitated from the biological sample, and as an external standard, an infectious bacteriophage particle comprising at least the pre-selected DNA sequence. | 12-25-2008 |
20080318205 | Hiv Type and Subtype Detection - The present invention relates to the detection of HIV by amplification and PCR-based methods. | 12-25-2008 |
20080318206 | DETECTION OF HERPES SIMPLEX VIRUS TYPES 1 AND 2 BY NUCLEIC ACID AMPLIFICATION - The present invention relates to a method of detecting the presence or absence of herpes simplex virus (HSV) in a sample based on amplifying a portion of the Glycoprotein G (US4) gene of HSV and detecting the presence of the amplified nucleic acid using primers and detector primers as described herewith. The method of the invention further identifies the type of HSV, either HSV-1 or HSV-2, in a sample. Also encompassed by the invention is a kit comprising the primers and detector primers which may be used with the amplification method described herewith. | 12-25-2008 |
20080318207 | SEQUENCE COVARIANCE NETWORKS, METHODS AND USES THEREFOR - Methods of identifying targets for designing a therapeutic agent are disclosed. These methods comprise: determining an amino acid sequence of one or more polypeptides of each isolate of a plurality of isolates of a biological system; identifying covariance pairs of amino acid residues; establishing a network comprising the covariance pairs; and identifying one or more hub residue positions, wherein a hub residue position comprises a target for designing a therapeutic agent if the hub residue position has a rank order in the 40 | 12-25-2008 |
20080318208 | Dengue Reporter Virus and Methods of Making and Using the Same - The present invention relates to the production and uses of Dengue virus replicons and Dengue reporter virus particles. The present invention relates to methods of identifying inhibitors of Dengue virus infection, inhibitors of Dengue virus replication, and inhibitors of Dengue virus assembly. | 12-25-2008 |
20090004643 | METHODS FOR CONCURRENT IDENTIFICATION AND QUANTIFICATION OF AN UNKNOWN BIOAGENT - The present invention provides methods for the quantification of an unknown bioagent in a sample by amplification of nucleic acid of the bioagent, and concurrent amplification of a known quantity of a calibration polynucleotide from which are obtained a bioagent identifying amplicon and a calibration amplicon. Upon molecular mass analysis, mass and abundance data are obtained. The identity of the bioagent is then determined from the molecular mass of the bioagent identifying amplicon and the quantity of the identified bioagent in the sample is determined from the abundance data of the bioagent identifying amplicon and the abundance data of the calibration amplicon. | 01-01-2009 |
20090004644 | METHODS AND COMPOSITIONS FOR PROCESSES OF RAPID SELECTION AND PRODUCTION OF NUCLEIC ACID APTAMERS - Embodiments herein relate to compositions and methods for making and using aptamers, for example, DNA aptamers (DCEs) and/or RNA aptamers. In some embodiments, methods relate to making and amplifying target DCEs. In certain embodiments, methods for making capture elements or aptamers concern using a reporter moiety and signal reducing moiety prior to amplifying a target-specific capture element. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of aptamers such as DCEs directed to a particular target agent. Some embodiments relate to systems for performing automated generation of aptamers. | 01-01-2009 |
20090011402 | BIOSENSORS BASED ON DIRECTED ASSEMBLY OF PARTICLES - A sensor system for detecting an effector or cofactor comprises (a) a nucleic acid enzyme; (b) a substrate for the nucleic acid enzyme, comprising a first polynucleotide; (c) a first set of particles comprising a second polynucleotide at least partially complementary to the substrate, where the polynucleotide is attached to the particles at its 3′ terminus; and (d) a second set of particles comprising a third polynucleotide at least partially complementary to the substrate, where the polynucleotide is attached to the particles at its 5′ terminus. | 01-08-2009 |
20090011403 | MICROPOROUS MATERIALS, METHODS OF MAKING, USING, AND ARTICLES THEREOF - Described herein are methods for separating one or more analytes present in a fluid sample. The methods involve passing the fluid through or into a microporous material, wherein the analytes are localized near the surface of the microporous material. Additional processing steps such as hybridization and amplification can be performed once the analyte is localized. In one method, once the analyte is localized, the analyte can be detected, counted, and correlated in order to determine the concentration of the analyte in the sample. In another method, the localized analyte is destabilized to make the localized analyte more accessible for chemical manipulation. Modified microporous materials and composite materials are also disclosed that can be used in any of the methods and articles described herein. The composite is composed of a microporous material and a pigment, wherein the pigment is incorporated in the microporous material. The pigments alter the optical properties of the microporous material, which enhances the detection of analyte once it is localized. Methods for making pigmented composites are also disclosed. In a further aspect, various kits and articles such as filtration devices containing any of the microporous materials described herein are provided. | 01-08-2009 |
20090017443 | Method for Detection of Hepatitus B Virus - To provide a method for detection or quantification of hepatitis B virus (HBV) antigens in serum and a simple and highly user-friendly method for sample treatment for use in the detection or quantification thereof. The method for treatment of a sample containing hepatitis B virus (HBV) is characterized in that release of HBV antigens and disruption of antibodies that bind to HBV antigens are carried out by treating a sample containing HBV with a treatment agent containing (1) an acidifying agent and (2) a protein denaturant or an amphoteric surfactant or cationic surfactant having an alkyl group and a tertiary amine or a quaternary ammonium salt within a molecule. | 01-15-2009 |
20090017444 | Screening method for modulators of viral transcription or replication - The invention provides methods to identify modulators of viral transcription or replication. | 01-15-2009 |
20090017445 | Method For Detecting And Removing Endotoxin - The present invention relates to bacteriophage tail proteins and the derivatives and fragments thereof that are capable of binding endotoxins in the absence of bivalent positive ions, especially Ca | 01-15-2009 |
20090017446 | Method and Set of Tools for Checking the Crystallisation Conditions of Biological Macromolecules - The invention relates to a method and set of tools for checking and ascertaining the crystallisation conditions of biological macromolecules using the counter-diffusion technique which employs precipitating agents, additives and buffers. The concentration of the precipitating agent(s) in the medium (solution or gel) is greater than those currently used with other available checking techniques, such as batch, microbatch or vapour phase diffusion techniques, such that, as a result of the diffusion along the length of the capillary containing the biological macromolecule, a large number of concentrations of the precipitating agent(s) used in one experiment are checked. The set of tools or kit contains the necessary elements for performing said method. | 01-15-2009 |
20090017447 | Human Endogenous Retrovirus with Foamy-Like Properties and Uses Thereof - The invention relates to the discovery of a human endogenous retrovirus (HERV) family, Type I HERV-K (HML-2) which appear to be active in vitro and in vivo, infectious, and which have the have the salient features and properties of foamy retroviruses. Based on its natural replication in humans, and that it protects the host from viral and tumor transformation, this non-pathogenic endogenous virus could be developed as a replication competent gene therapy vector. It also is expected to have much higher efficacy than other vectors as it crosses the bloodbrain barrier and infects almost all cell types in the host (proliferating or not). It may naturally lyse tumor cells or infected cells, and thus could even be used without genetic modification. Of course, this vector could be used in traditional ways with it ability to replicate genetically removed. In addition to its value as a vector, as it is reactivated with infection, its detection could also be used to monitor the safety of gene therapy (irrespective of vector type used), as well as other biological therapies including vaccination, blood transfusion, transplantation and xenotransplantation. Finally it may be used to screen for new therapeutic and prophylactic treatments for a wide variety of diseases. | 01-15-2009 |
20090017448 | SCREENING TOOL FOR ANTIVIRAL AGENTS - A method is provided for screening anti-adenovirus agents. The method includes reducing the activation of the immune system of a small mammal, administering a human adenovirus vector to the small mammal, monitoring the tumor cells in the mammal, and analyzing infectious virus units within the tumor cells and the organs of the small mammal. Specifically, the immune system of the small mammal is suppressed using cyclophosphamide. The small mammal may be, but is not limited to, one of the following: mice, rabbits, cotton rats, hamsters, rats, and other small rodents. | 01-15-2009 |
20090017449 | COMPOUNDS AND METHODS FOR ASSAYING FUSION OF AN INDIVIDUAL, ENVELOPED VIRUS WITH TARGET MEMBRANE - Compositions and methods for monitoring viral fusion are provided. Methods of labelling virions are also provided. A novel, detectable label is provided. A mobile lipid bilayer is also provided. | 01-15-2009 |
20090023133 | GENOMIC MARKERS OF HEPATITIS B VIRUS ASSOCIATED WITH HEPATOCELLULAR CARCINOMA - The present invention provides methods of predicting a pre-disposition of HBV-infected individuals to develop hepatacellular carcinoma (HCC). | 01-22-2009 |
20090029346 | Detection of human papilloma virus - An assay for detecting HPV comprising treating the viral nucleic acid with an agent that modifies cytosine to form derivative viral nucleic acid, amplifying at least a part of the derivative viral nucleic acid to form an HPV-specific nucleic acid molecule, and looking for the presence of an HPV-specific nucleic acid molecule, wherein detection of the HPV-specific nucleic acid molecule is indicative HPV. | 01-29-2009 |
20090029347 | Method for Identifying Multiple Analytes Using Flow Cytometry - The present disclosure is directed to a method of using antibodies attached to different types of microspheres against different antigens located within a biological sample. Optical and electronic particle detection may be used to separate the microspheres via flow cytometry, allowing the subsequent measurement of multiple analytes in a single sample of body fluid by separating and gating such analytes based on the type of microsphere to which the analyte is coupled. According to the present disclosure, various biological components may be attached to microspheres of different volumes, shapes, conductivity, densities, and/or colors to detect biological components by gating on the type of microsphere and analyzing the biological component attached thereto. | 01-29-2009 |
20090029348 | Kit for Detecting the Antibody of HCV and Its Preparing Method - A kit and its preparing method concerning dual-antigen sandwich method are used for detecting the antibody against HCV, and its detecting mode is ‘carrier-first antigen-antibody against HCV to be detected-second antigen-marker-distinguishable signal’. The kit ant its preparing method characterize in that the second antigen is the complex of a HCV and a tag. | 01-29-2009 |
20090029349 | Novel Applications of Acridinium Compounds and Derivatives in Homogeneous Assays - Chemiluminescent acridinium compounds are used in homogeneous assays to determine the concentration of an analyte in a sample without strong acid or strong base treatment. The chemiluminescent acridinium compounds include acridinium esters with electron donating functional groups at the C2 and/or C7 position on the acridinium nucleus to inhibit pseudo-base formation, or acridinium sulfonamides with or without electron donating functional groups at the C2 and/or C7 position on the acridinium nucleus. | 01-29-2009 |
20090029350 | RAPID DIAGNOSTIC METHOD FOR DISTINGUISHING ALLERGIES AND INFECTIONS AND NASAL SECRETION COLLECTION UNIT - A method for clinical scoring that involves evaluating certain parameters found in nasal secretia is disclosed. Contact of a nasal secretion with indicators for pH, protein content, leukocyte esterase activity, nitrite content, eosinophil content and/or TAME esterase activity permits diagnosis and differentiation between allergic, bacterial and viral conditions. | 01-29-2009 |
20090029351 | METHOD OF MEASURING HUMAN CYP3A INDUCIBILITY - A method for measuring human CYP3A inducibility upon administration of a test drug, characterized in that a non-human animal to which a test drug is administered or a population of human cells cultured in a medium containing a test drug is infected with viruses (A) and (B); virus (A) being an adenovirus which is used as a vector and engineered by incorporating thereto a detectable reporter gene and at least 3 human PXR binding regions falling within an untranslated region of a human CYP3A gene, and virus (B) being an adenovirus which is used as a vector and engineered by incorporating thereto a human PXR cDNA; and subsequently expression level of the reporter gene is determined in the non-human animal or the cultured human cells. | 01-29-2009 |
20090029352 | Method for detecting the Presence of A Nucleic Acid in A Sample - An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations, or modules, in which discrete aspects of the assay are performed on fluid samples contained in reaction receptacles. The analyzer includes stations for automatically preparing a specimen sample, incubating the sample at prescribed temperatures for prescribed periods, performing an analyte isolation procedure, and ascertaining the presence of a target analyte. An automated receptacle transporting system moves the reaction receptacles from one station to the next. The analyzer further includes devices for carrying a plurality of specimen tubes and disposable pipette tips in a machine-accessible manner, a device for agitating containers of target capture reagents comprising suspensions of solid support material and for presenting the containers for machine access thereto, and a device for holding containers of reagents in a temperature controlled environment and presenting the containers for machine access thereto. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte. The process is performed by automatically moving each of a plurality of reaction receptacles containing a solid support material and a fluid sample between stations for incubating the contents of the reaction receptacle and for separating the target analyte bound to the solid support from the fluid sample. An amplification reagent is added to the separated analyte after the analyte separation step and before a final incubation step. | 01-29-2009 |
20090035747 | Nucleic Acid and Gene Derived from Novel HCV Strain and Replicon-Replicating Cell Using Said Gene - The present invention relates to a gene derived from a novel fulminant hepatitis C virus strain, an HCV replicon RNA with a high replication efficiency obtained using the gene, and an HCV replicon-replicating cell transfected with the replicon RNA. When the HCV replicon RNA and the HCV replicon-replicating cell of the present invention are used, HCV proteins can be continuously produced in a large amount. | 02-05-2009 |
20090035748 | BROMELAIN AS A CLINICAL SAMPLE PRE-TREATMENT, LYSIS AGENT AND NUCLEASE INHIBITOR - This invention generally relates to the use of the proteolytic enzyme bromelain for treating samples for diagnostic assays. More specifically, the present invention relates to the use of bromelain in methods for pre-treating clinical samples primarily for the purpose of obtaining extractable and amplifiable DNA or RNA from prokaryotic or eukaryotic cells and/or viruses. The present methods also relate to using bromelain as a nuclease inhibitor. The use of bromelain as a nuclease inhibitor is useful in applications where the destructive nature of nucleases are detrimental to downstream applications. This invention also relates to the use of bromelain as a lysis agent for samples to be used, for example, in a molecular based diagnostic assay. The present methods include treating a sample which contains bacteria, viruses, host cells, fungi or parasites with bromelain, typically extracting nucleic acid from the sample, and subjecting the sample to a nucleic acid-based diagnostic assay. | 02-05-2009 |
20090035749 | ANALYSIS OF HIV-1 CORECEPTOR USE IN THE CLINICAL CARE OF HIV-1 INFECTED PATIENTS - A change in viral tropism occurs in many HIV positive individuals over time and can be indicated by a shift in coreceptor use from CCR5 to CXCR4. The shift in coreceptor use to CXCR4 has been shown to correlate with increased disease progression. In patients undergoing HAART, the predominant populations of virus can be shifted back to CCR5-mediated entry after the CXCR4-specific strains have emerged. The present invention relates to a diagnostic method to monitor coreceptor use in the treatment of human immunodeficiency virus (HIV) infection. The present invention further relates to a diagnostic method applied to HIV-positive individuals undergoing HAART to monitor the suppression of CXCR4 specific strains. The diagnostic methods can be used to assist in selecting antiretroviral therapy and to improve predictions of disease prognosis over time. | 02-05-2009 |
20090035750 | METHOD OF DETECTING HUMAN PAPILLOMA VIRUS BY USING NUCLEIC ACID AMPLIFICATION METHOD AND NUCLEIC ACID CHAIN-IMMOBILIZED CARRIER - Provided is a nucleic acid primer for LAMP amplification for use in the detection of human papilloma virus and identification of its genotype. The present invention also provides a method of detecting human papilloma virus and identifying its genotype, includes a step of amplifying the nucleic acid chains in a sample in LAMP reaction by using multiple primers including at least one primer selected from the nucleic acid primers according to the present invention and a step of detecting presence of amplified products after the amplification reaction and identifying their genotypes. | 02-05-2009 |
20090042181 | Nucleic acid and gene derived from novel HCV strain and replicon-replicating cell using said gene - The present invention relates to a gene derived from a novel fulminant hepatitis C virus strain, an HCV replicon RNA with a high replication efficiency obtained using the gene, and an HCV replicon-replicating cell transfected with the replicon RNA. When the HCV replicon RNA and the HCV replicon-replicating cell of the present invention are used, HCV proteins can be continuously produced in a large amount. | 02-12-2009 |
20090042182 | DETECTION OF BOVINE VIRAL DIARRHEA VIRUS IN TISSUE SAMPLES - The present invention relates to a method of detecting whether a target animal is Bovine Viral Diarrhea Virus (BVDV) positive or negative by determining whether a gp48 protein-specific reagent binds to a gp48 Bovine Viral Diarrhea Virus protein or protein fragment, which retains antigenic specificity, from a target animal's tissue sample. | 02-12-2009 |
20090047656 | Molecular analysis of primary cells - The present invention provides a method of propagating cells of interest obtained from a biological specimen by a) enriching the cells under conditions that maintain sufficient cell viability; and b) propagating the cells under conditions effective to allow cell viability, proliferation and integrity. | 02-19-2009 |
20090047657 | Detection Method For Latent Viral Infections and Its Kit For Examination - The subject of the present invention is to provide a detection method for latent viral infections by detecting a gene product related to latent infections without going through an invasive procedure involving pain and bleeding, caused by skin biopsy and blood collection. Further, the subject of the present invention is to provide a kit for examination using in the detection of latent viral infections described above. As crusts and scales in a lesion contain large amounts of virus-infected cells which are in a state of dry necrosis, the method collects crusts and/or scales for a test sample and detects a gene product related to latent infections which may be present in the test sample. The kit for examination comprises (1) antisense oligonucleotide for reverse transcription of a gene product related to latent viral infections, (2) a primer set for amplifying a gene product related to latent viral infections and (3) a primer set for amplifying a housekeeping gene. | 02-19-2009 |
20090047658 | Methods and compositions for determining the pathogenic status of infectious agents - Methods and compositions for the detection of disease caused by infectious agents and microbes are provided. In particular, methods and compositions comprising novel combinations of nucleic acid amplification and drug susceptibility technologies are provided. In certain embodiments, the present invention enables the detection of infectious agents and microbes as well as providing information concerning the viability status of the agent or microbe. In one embodiment, the present invention is used for the detection of mycobacterial infections, including, but not limited to, tuberculosis. | 02-19-2009 |
20090047659 | AMPLIFICATION OF HIV-1 SEQUENCES FOR DETECTION OF SEQUENCES ASSOCIATED WITH DRUG-RESISTANCE MUTATIONS - Sequences of nucleic acid oligonucleotides for amplifying different portions of gag and pol genes of HIV-1 and for detecting such amplified nucleic acid sequences are disclosed. Methods of amplifying and detecting HIV-1 nucleic acid in a biological sample using the amplification oligonucleotides specific for gag and pol target sequences are disclosed. | 02-19-2009 |
20090047660 | Antibodies for oncogenic strains of HPV and methods of their use - The subject invention provides antibodies, including polyclonal and monoclonal antibodies, that bind to E6 proteins from at least three oncogenic strains of HPV. In general, the antibodies bind to amino acids motifs that are conserved between the E6 proteins of different HPV strains, particularly HPV strains 16 and 18. The subject antibodies may be used to detect HPV E6 protein in a sample, and, accordingly, the antibodies find use in a variety of diagnostic applications, including methods of diagnosing cancer. Kits for performing the subject methods and containing the subject antibodies are also provided. | 02-19-2009 |
20090047661 | QUANTITATIVE HIV PHENOTYPE OR TROPISM ASSAY - The present invention concerns a method for predicting quantitative phenotype, e.g. gag-phenotype, integrase phenotype or tropism in a patient infected by Human Immunodeficiency Virus (HIV). | 02-19-2009 |
20090047662 | HETERODUPLEX TRACKING ASSAY - A change in viral tropism occurs in many HIV positive individuals over time and may be indicated by a shift in coreceptor use from CCR5 to CXCR4. The shift in coreceptor use to CXCR4 has been shown to correlate with increased disease progression. In patients undergoing HAART, the predominant populations of virus may be shifted back to CCR5-mediated entry soon after the CXCR4-specific strains have emerged. The present invention relates to a diagnostic method to monitor coreceptor use in the treatment and clinical management of human immunodeficiency virus (HIV) infection. The present invention further relates to a diagnostic method applied to HIV-positive individuals undergoing HAART to monitor the suppression of CCR5- or CXCR4-specific strains. The diagnostic methods may be used to assist in selecting antiretroviral therapy and to improve predictions of disease prognosis over time. The methods of the invention include cell-based methods, including cell fusion assays, and molecular-based methods, including heteroduplex tracking assay, to both quantitatively and qualitatively analyze patient-derived HIV for coreceptor usage. | 02-19-2009 |
20090047663 | Methods and Compositions For Identifying and Characterizing Hepatitis C - The invention provides novel methods and compositions for amplifying portions of the HCV genome. The nucleic acid sequences set forth as SEQ ID NOS:1-64 derived from HCV cDNA and functional equivalents thereof, kits containing same, and methods employing same, are useful for the identification and characterization of HCV in biological samples. | 02-19-2009 |
20090047664 | SPECIES DETECTION METHODS AND SYSTEMS - The disclosure provides methods, systems and kits for cellular and subcellular identification in a rapid, throughput manner. | 02-19-2009 |
20090047665 | COMPOSITIONS FOR USE IN IDENTIFICATION OF ADENOVIRUSES - The present invention provides compositions, kits and methods for rapid identification and quantification of adenoviruses by molecular mass and base composition analysis. | 02-19-2009 |
20090053687 | METHOD FOR THE DETECTION OF HPV AND PROBES, PRIMERS AND KITS - The invention relates to materials and methods method for detection and/or typing of any HPV nucleic acid possibly present in a biological sample, the method comprising the steps of: (i) amplification of a polynucleic acid fragment comprising or consisting of the B region of any HPV nucleic acid in the sample, said B region being indicated in FIG. | 02-26-2009 |
20090053688 | METHOD AND DEVICE FOR ULTRASOUND ASSISTED PARTICLE AGGLUTINATION ASSAY - Ultrasound-assisted particle agglutination assay methods and apparatuses are described based on first providing a standing wave ultrasound field at a resonance frequency of a test liquid in a resonator cell containing microparticles covered with a binding agent with high affinity to an analyte sought to be detected by the assay test. Formation of the specifically-bound and nonspecifically-bound aggregates of these microparticles is then followed by effective stirring of the liquid with swept-frequency sonication causing disintegration of nonspecifically-bound aggregates and leaving specifically-bound aggregates in place for further detection and measurement. The methods and devices of the invention allow significant improvement in the sensitivity and specificity of agglutination tests and are advantageously applicable to detecting various proteins, DNA, RNA and other biologically active substances. Specific examples are provided. | 02-26-2009 |
20090053689 | DEVICE FOR PROCESSING A BIOLOGICAL AND/OR CHEMICAL SAMPLE AND METHOD OF USING THE SAME - The present invention relates to a device and an apparatus device for processing a biological and/or chemical sample. The device comprises at least one sample processing chamber having an inlet at a first end and a penetrable sealing layer at a second end that forms at least a part of an inner wall of the sample processing chamber. The sealing layer is adapted to seal off the sample processing chamber from the environment until said sealing layer is penetrated to form an outlet. The device and apparatus also includes an absorption layer, wherein upon penetration of said sealing layer, said absorption layer is in fluid contact with said sealing layer and is capable of absorbing fluid released from said sample processing chamber, via the outlet. The present invention also relates to a fluid separation device. The fluid separation device comprises a penetrable sealing layer adapted to form a wall of a sample processing chamber of the device of the invention. The fluid separation device also comprises an absorption layer that is in fluid contact with the sealing layer and capable of absorbing fluid released from a sample processing chamber of the device. | 02-26-2009 |
20090053690 | SURFACE CHEMISTRY AND DEPOSITION TECHNIQUES - Surface chemistries for the visualization of labeled single molecules (analytes) with improved signal-to-noise properties are provided. To be observed, analyte molecules are bound to surface attachment features that are spaced apart on the surface such that when the analytes are labeled adjacent analytes are optically resolvable from each other. One way to express this concept is that binding elements should be spaced apart such that the Guassian point spread functions of adjacent labels do not overlap. Another way of expressing this concept is that the surface binding elements should be spaced apart by a distance equal to at least the diffraction limit for an optical label attached to the bound analytes. | 02-26-2009 |
20090053691 | Cell Line and Methods for Determining Viral Titer - The present invention relates to cells, methods, compositions and kits for determining the concentration of virus in a stock, i.e., determining the titer of a viral stock. | 02-26-2009 |
20090053692 | DETECTION OF HIV-1 BY NUCLEIC ACID AMPLIFICATION - Nucleic acid sequences and methods for detecting HIV-1 nucleic acid (LTR and pol sequences) in biological samples by detecting amplified nucleic acids are disclosed. Kits comprising nucleic acid oligomers for amplifying HIV-1 nucleic acid present in a biological sample and detecting the amplified nucleic acid are disclosed. | 02-26-2009 |
20090061412 | Methods for Detecting Papillomavirus DNA in Blood Plasma and Serum - This invention relates to the detection of extracellular papillomavirus DNA in blood plasma or serum from a human or animal. In particular, the invention relates to the detection, identification, evaluation, or monitoring of neoplastic, premalignant or malignant disease associated with a papillomavirus. The invention thereby provides methods for the identification of individuals at risk for, or having, cervical dysplasia, cervical intraepithelial neoplasia, or cervical cancer. | 03-05-2009 |
20090061413 | ISOTHERMAL SCREENING OF HIV-1 RELATED NUCLEIC ACIDS - The presently described technology relates generally to the art of molecular diagnostics and more particularly to point-of-care diagnostic methods and materials. The diagnostic methods and materials of the presently described technology are suitable for a variety of uses including but not limited to the bedside or field diagnosis of infectious or noninfectious diseases. | 03-05-2009 |
20090061414 | Method for quantification of recombinant viruses - Titration is an important and critical step in dosing recombinant virus for gene therapy. A relatively fast, convenient and sensitive method that allows for precise quantification of recombinant retrovirus is presented. The method is based on PCR amplification of a foreign gene by the PRINS (primer in situ DNA synthesis) technique. The PRINS technique is based on the sequence-specific annealing of unlabeled oligonucleotide DNA it situ. This oligonucleotide operates as a primer for in situ chain elongation catalyzed by the Taq I polymerase. Using -labeled nucleotides as a substrate for chain elongation, the neo-synthetic DNA is labeled by an FITC-conjugated anti-antibody. To avoid the possibility of false positives, the puromycin resistance gene, which is associated with the transgene in the same viral vector and is not normally present in mammalian cells was amplified. The retroviral titer was evaluated by counting FITC-positive cells after PRINS labeling, while knowing the number of cells that were transduced with different amounts of viral supernatant. A comparable viral concentration of 1×10 | 03-05-2009 |
20090061415 | Sequences Diagnostic For Shrimp Pathogens - Primers have been isolated that are diagnostic for the detection of the taura syndrome virus (TSV). The primers are based on a new portion of the TSV genome and may be used in primer directed amplification or nucleic acid hybridization assay methods. | 03-05-2009 |
20090061416 | Surfaces and methods for biosensor cellular assays - Disclosed is an apparatus for measuring ligand-induced cell activity as defined herein, the apparatus including: an optical biosensor having a contact surface including a compatibilizer zone, an optional surface modifier zone, and a live cell zone. The disclosure also provides a method of making the apparatus and methods for measuring ligand-induced live cell activity with the apparatus. | 03-05-2009 |
20090061417 | INFLUENZA A VIRUS DETECTION METHOD AND KIT THEREFORE - The invention provides oligonucleotides for a simple, specific and/or sensitive test for the presence of Influenza A. In particular, the present invention provides a primer(s), probe(s) and/or test(s) for Influenza A Subtype H5N1. Kits comprising probe(s) and/or primer(s) useful in the test are also provided. | 03-05-2009 |
20090061418 | DISPOSABLE ANALYTICAL MICROPROCESSOR DEVICE - The present invention generally relates to the determination of an analyte concentration (quantitative determination) or whether an analyte threshold level has been passed (qualitative determination) in a biological sample through employment of a disposable analytical microprocessor device. The device can include a batch-specific, self-executable algorithm for the calculation of the analyte concentration. | 03-05-2009 |
20090061419 | GENOMIC MARKERS OF HEPATITIS B VIRUS ASSOCIATED WITH HEPATOCELLULAR CARCINOMA - The present invention provides methods of predicting a pre-disposition of HBV-infected individuals to develop hepatacellular carcinoma (HCC). | 03-05-2009 |
20090061420 | MUTATIONAL PROFILES IN HIV-1 PROTEASE CORRELATED WITH PHENOTYPIC DRUG RESISTANCE - The present invention is directed to the field of nucleic acid diagnostics and the identification of base variation in target nucleic acid sequences. More particularly, the present invention relates to the use of such genotypic characterization of a target population of HIV and the subsequent association, i.e., correlation, of this information to phenotypic interpretation in order to correlate virus mutational profiles with drug resistance. The invention also relates to methods of utilizing the mutational profiles of the invention in drug development, i.e., drug discovery, drug design, drug modification, and therapy, treatment design, clinical management and diagnostic analysis | 03-05-2009 |
20090068635 | INDIRECTLY LABELLED ASSAY CONJUGATES AND METHODS OF PREPARING AND USING SAME - Indirectly labelled assay conjugates prepared by a method that includes the step of submitting the binding member comprised by the conjugate to denaturing conditions prior to labelling the binding member. The indirectly labelled assay conjugates demonstrate an increased sensitivity when employed in diagnostic assays compared to assay conjugates prepared by methods that do not include a step of submitting the binding member to denaturing conditions prior to labelling. Processes for the preparation of the indirectly labelled assay conjugates, methods of detecting an analyte comprising the use of the indirectly labelled assay conjugate and kits comprising the indirectly labelled conjugates are also provided. | 03-12-2009 |
20090068636 | VIRAL PROTEIN - The present invention is directed to an isolated polypeptide containing SEQ ID NO: 1 or an immunogenic fragment thereof. Also disclosed is an isolated nucleic acid encoding the polypeptide or containing a sequence at least 70% identical to SEQ ID NO: 3. Within the scope of this invention are related expression vectors, host cells, and antibodies. Also disclosed are methods of producing the polypeptide, diagnosing coronavirus infection, and identifying a test compound for treating coronavirus infection. | 03-12-2009 |
20090068637 | MONOCLONAL ANTIBODIES BINDING TO AVIAN INFLUENZA VIRUS SUBTYPE H5 HAEMAGGLUTININ AND USE THEREOF - The present application provides monoclonal antibodies that specifically bind to the hemagglutinin of avian influenza virus subtype H5, as well as monoclonal antibodies capable of blocking at least 50% of the hemagglutinin binding activity of these monoclonal antibodies. Such antibodies are useful, for example, in the detection, diagnosis, prevention, and treatment of avian influenza virus. Also provided herein are hybridoma cell lines, isolated nucleic acid molecules, and short peptides related to the monoclonal antibodies provided herein, and pharmaceutical compositions and kits containing the monoclonal antibodies provided herein. | 03-12-2009 |
20090068638 | PHAGE-BASED METHOD FOR THE DETECTION OF BACTERIA - The present invention relates to the field of biosensors useful for detecting bacteria. More particularly, the present invention relates to an electrochemical cell or biosensor and its use in a phage-based method and kit for the detection of bacteria. | 03-12-2009 |
20090068639 | System and method of quantitatively determining a biomolecule, system and method of detecting and separating a cell by flow cytometry, and fluorescent silica particles for use in the same, and kit comprising plural kinds of the silica particles in combination - A system of quantitatively determining a biomolecule, which has: allowing fluorescent silica particles capable of emitting fluorescence detectable by a flow cytometer to capture a target biomolecule fluorescent-labelled for quantitative determination; detecting the fluorescence emitted from the fluorescent silica particles themselves by using the flow cytometer; and measuring the intensity of the fluorescence of the labelled target biomolecule, thereby quantitatively determining the target biomolecule. | 03-12-2009 |
20090068640 | METHODS FOR DETECTING AND INACTIVATING A PRION - A method for the isolation or detection of a prion in a sample is disclosed. Also disclosed are methods for the disinfection and/or decontamination and/or inactivation of TSE infectivity. | 03-12-2009 |
20090075250 | Environmental Sampling and Testing Method - Provided is sampling and testing device for the detection of specific molds, allergens, viruses, bacteria, fungi, and other protein containing substances. Embodiments of the device include a sampling member slideably engaged with a base that contains a lateral flow strip adapted to detect specific analytes of interest. The sampling member defines a solvent reservoir that stores an elution solvent in a fluid-tight manner before the device is used to sample and test environmental surfaces. During slideable withdrawal of the sampling member from the base, the elution solvent stored in the reservoir is automatically released to a wick assembly of the sampling member. The wick assembly includes a wick adapted to receive, distribute, and retain the elution solvent. After a user samples an environmental surface for an analyte of interest with the elution solvent wetted wick, the sampling member is returned to the base where the wick contacts the lateral flow strip contained in the base. The wick transfers at least a portion of analyte and the elution solvent to the lateral flow strip for the calorimetric detection of specific allergens, viruses, bacteria, and other protein containing substances in the sample. The calorimetric results of the test are displayed through a window in the base. | 03-19-2009 |
20090081636 | Pharmaceutical compositions for and methods of inhibiting HCV replication - The present invention relates generally to replicase complex defect inducers and pharmaceutical compositions containing such inducers. Methods of developing mutants that are resistant to replicase complex defect inducers are also provided. Further included are mutants that can be used in screening for replicase complex defect inducers. Methods of screening test compounds for the ability to induce the formation of replicase complex defects are also described. Also included are methods of inhibition of HCV replication by replicase complex defect inducers. | 03-26-2009 |
20090081637 | Hepatitis B Viral Variants With Reduced Susceptibility To Nucleoside Analogs And Uses Thereof - The present invention relates generally to viral variants exhibiting reduced sensitivity to particular agents and/or reduced interactivity with immunological reagents. More particularly, the present invention is directed to hepatitis B virus (HBV) variants exhibiting complete or partial resistance to nucleoside or nucleotide analogs and/or reduced interactivity with antibodies to viral surface components including reduced sensitivity to these antibodies. The present invention further contemplates assays for detecting such viral variants, which assays are useful in monitoring anti-viral therapeutic regimens and in developing new or modified vaccines directed against viral agents and in particular HBV variants. The present invention also contemplates the use of the viral variants to screen for and/or develop or design agents capable of inhibiting infection, replication and/or release of the virus. | 03-26-2009 |
20090081638 | Immobilisation of Antigenic Carbohydrates to Support Detection of Pathogenic Microorganisms - The invention relates to the field of chemistry and diagnosis, more in particular to diagnosis of current and/or past and/or symptomless infections or of a history of exposure to, a gram-negative-bacterium (such as an enterobacteriaceae or a legionella). Even more in particular, the invention relates to the screening of animals or animal products for the presence of unwanted/undesired microorganisms. The invention further relates to a method for screening samples for the presence of antibodies directed against unwanted/undesired microorganisms and preferably such a method is performed with help of a biosensor. The invention also relates to a method for immobilizing polysaccharides to solid surfaces. The invention furthermore provides solid surfaces with immobilized polysaccharides as well as applications of such surfaces. | 03-26-2009 |
20090081639 | Assay for sensitivity to chemotherapeutic agents - Diagnostic methods for assaying the efficacy of chemotherapeutic agents in vitro for the treatment of cancer and methods for identifying chemotherapeutic agents are provided. The methods employ reporter viruses. Combinations and kits for use in the practicing the methods are also provided. | 03-26-2009 |
20090081640 | Methods of using miRNA for detection of in vivo cell death - Described are non-invasive methods of detecting in vivo cell death by measuring levels of ubiquitous and tissue specific miRNA. The method can be applied for detection of pathologies caused or accompanied by cell death, as well as for diagnosis of infectious disease, cytotoxic effects induced by different chemical or physical factors, and the presence of specific fetal abnormalities. | 03-26-2009 |
20090081641 | Methods and systems for treating disease - Methods and systems described herein are applicable to the identification of pathogens, pathogenic variants and applicable treatments or remedies. In some embodiments, the pathogen or pathogens bears a causal relationship to a disease state. | 03-26-2009 |
20090087831 | METHODS FOR EVALUATING A BACTERIOPHAGE PREPARATION - A quality assurance/quality control paradigm for bacteriophage is provided. | 04-02-2009 |
20090087832 | Nucleic acids and new polypeptides associated with and/or overlapping with hepatitis C virus core gene products - DNA encoding core+1 polypeptides of hepatitis C virus (HCV), nucleotides encoding the polypeptides, and methods for using the nucleotides and the encoded polypeptides are disclosed. | 04-02-2009 |
20090087833 | ADIPOGENIC ADENOVIRUSES AS A BIOMARKER FOR DISEASE - This invention relates to the relationship between infection with an adipogenic adenovirus, such as adenovirus-36, and obesity-related disease. In particular, this invention relates to assaying a subject to determine the adipogenic adenovirus infection status and then determining the subject's predisposition to developing an obesity-related disease based on the adipogenic adenovirus infection status. | 04-02-2009 |
20090092962 | METHOD FOR DETECTING SARS CORONAVIRUS - This invention provides: a method for detecting SARS pathogenic viruses with high sensitivity and rapidity for diagnosing severe acute respiratory syndrome (SARS); an oligonucleotide primer that can specifically hybridize with any nucleotide sequence constructed based on the nucleotide sequence of RNA polymerase of the SARS coronavirus; a method for nucleic acid amplification using such primer; a method for diagnosing infection with the SARS coronavirus via detection of nucleic acid amplification; and a kit for diagnosing SARS. | 04-09-2009 |
20090092963 | Method for combined parallel agent delivery and electroporation for cell structures an use thereof - Disclosed is a method for parallel delivery of agents to and/or into a cell structure, wherein at least two electrolyte-filled tubes are provided together with a counter electrode, the tubes being connected to a voltage or current generator, said agents being introduced into the electrolyte solution contained in the tubes, which are placed close to the cell structure, whereupon the agents are transported through the tubes to said cell structure and into the said structure through pores which have been formed by application of an electric field focused on the cell structure, resulting in electroporation of the cell structure. Also different applications of the method is disclosed, e.g. use of the method in order to transfer cell-impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure. | 04-09-2009 |
20090092964 | Methods for Individualizing Cardiovascular Disease Treatment Protocols Based on Beta-1 Adrenergic Receptor Haplotype - A method is provided for determining whether a treatment protocol for a human patient who is suffering from heart failure, ischemic heart disease, cardiac arrhythmias, or hypertension includes administration of a beta blocker, the method including obtaining a biological sample from the patient, determining a β | 04-09-2009 |
20090092965 | Method and Apparatus for Target Detection Using Electrode-Bound Viruses - A biosensor capable of detecting the presence and/or concentration of an analyte or biomarker includes at least one electrically conductive electrode operatively coupled to an impedance analyzer for measuring the change in the resistive impedance of the electrode in response to an applied alternating current at a plurality of frequencies. In one embodiment, at least one electrode is covered with a self-assembled monolayer that is chemically bonded to a surface. A plurality of virus particles such as phage viruses are immobilized on the self-assembled monolayer and may be exposed to a test or sample solution. The virus particles may be obtained from phage-displayed libraries to detect a wide variety of targets including, for example, DNA, RNA, small molecules, and proteins or polypeptides. In another embodiment, the virus particles are electrostatically bound to a substrate in between a pair of elongated electrodes disposed on a substrate. | 04-09-2009 |
20090098527 | BIOLOGICAL ORGANISM IDENTIFICATION PRODUCT AND METHODS - A biological organism identification product, and methods of using the same, that include a collection device to collect one or more sample organisms, a fixing and transporting composition present in an amount sufficient to kill the sample organism(s) associated with the collection device, an extraction member to extract a sufficient amount of genomic nucleic acid from the sample organism(s) to facilitate identification; and a polymerase chain reaction component into which the sufficient amount of genomic nucleic acid can be dissolved. The amplified genomic material is exposed to molecules that bind to predetermined genomic sequences, providing the identification feature of the product. The biological organism identification product may be portable, durable, and self-contained. | 04-16-2009 |
20090098528 | Method for determining early HCV seroconversion - The present invention concerns a polypeptide which is composed of the amino acids 1207±10 to 1488±10 of a hepatitis C virus and of less than 20 foreign amino acids and the use of this polypeptide as an antigen in an immunological test. | 04-16-2009 |
20090098529 | Methods for attenuating virus strains for diagnostic and therapeutic uses - Modified or attenuated viruses and methods for preparing the modified viruses and modulating attenuation are provided. Vaccines that contain the viruses are provided. The viruses can be used in methods of treatment of diseases, such as proliferative and inflammatory disorders, including as anti-tumor agents. The viruses also can be used in diagnostic methods. | 04-16-2009 |
20090098530 | Cell Line For Producing Coronaviruses - The invention relates to the production of coronaviruses. In particular, the invention relates to methods for producing SARS-CoV by using cells expressing a functional SARS-CoV receptor | 04-16-2009 |
20090098531 | DETECTING HEPATITIS B VIRUS - This invention is related to methods for detecting hepatitis B virus by determining the level of hepatitis B virus surface antigen protein. This invention is also related to methods for detecting mutant hepatitis B virus surface antigen protein and kits for detecting hepatitis B virus. | 04-16-2009 |
20090098532 | Probes and Methods for Hepatitis C Virus Typing Using Single Probe Analysis - This invention provides compositions and methods for HCV typing, e.g., genotyping and/or subtyping. The compositions and methods of the invention can be used to assign an HCV isolate to one of at least five HCV genotypes (for example, selected from genotypes 1, 2, 3, 4, 5 or 6), or assign an HCV isolate to one of at least six subtypes (for example, selected from subtypes 1a/b/c, 2a/c, 2b, 3a, 4a, 5a or 6a), where the methods of the invention use only a single typing probe to make the HCV type assignment. | 04-16-2009 |
20090104595 | Methods for determining the sensitivity or resistance of retrovirus isolates to therapeutic retroviral treatments based on viral protease inhibitors and diagnostic kits - A method for determining sensitivity or resistance of isolates of HIV (human immunodeficiency virus) retroviruses to chemical molecules having an inhibiting activity on a viral protease or to therapeutic treatments based on inhibitors of the viral protease, including causing cell lysis of at least one yeast by expression of the retrovirus protease. | 04-23-2009 |
20090104596 | Noninvasive Measurement and Identification of Biomarkers in Disease State - The invention is methods and related kits for diagnosing a disease state of cachexia by measuring biomarker profiles from a biological sample. Rapid measurement of early onset or progression of the disease in a subject is determined by measuring biomarker levels from the subject and optionally comparing the biomarker levels to a standard biomarker profile or metabolome phase portrait for the disease. The biomarkers measured in the assay and related kit for cachexia progression include biomarkers selected from the group consisting of lactate, citrate, formate, acetoacetate, 3-hydroxy butrate, alanine, glutamine, glutamate, valine, isoleucine leucine, thrionine, lysine, arginine, tyrosine, phenyl alanine, histidine and tryptophan. | 04-23-2009 |
20090104597 | ADVANCED CERVICAL CELL SCREENING METHODS - Advanced cervical cancer screening methods that provide a molecular based process of detecting HPV-integration. The disclosed methods allow for a streamlined approach of conducting a Pap test and immunohistochemical test on the same slide. The disclosed methods provides an inexpensive, highly sensitive, specific, and detailed test that is easy to evaluate and follow-up. | 04-23-2009 |
20090111088 | RAPID ASSESSMENT OF UPPER RESPIRATORY CONDITIONS - A method for rapidly assessing upper respiratory conditions is provided. More specifically, the method involves contacting a sample obtained from the upper respiratory tract of a host with a test strip. The test strip contains an indicator that provides a broad spectrum response in the presence of bacteria, mold, yeast, or other microorganisms that is different than its response in the presence of viruses. This allows for a rapid and simple assessment as to whether the test sample is infected with a virus or some other microorganism. To help a clinician identify the proper course of treatment, it may also be desirable to obtain further information about the particular type of microorganism present. In this regard, the test strip contains any array of one or more differentiating indicators that provides a certain spectral response in the presence of different types of microorganisms. For example, the array may provide a certain spectral response in the presence of gram-negative bacteria, but a completely different spectral response in the presence of gram-positive bacteria. Likewise, the array may provide a certain spectral response in the presence of Rhinoviruses (associated with the common cold), but a different response in the presence of Influenza viruses. Detection of the spectral response provided by the indicators may thus allow for rapid differentiation between different types of microorganisms. | 04-30-2009 |
20090111089 | PRIMERS AND PROBES FOR DETECTION AND DISCRIMINATION OF TYPES AND SUBTYPES OF INFLUENZA VIRUSES - Methods of detecting influenza, including differentiating between type and subtype are disclosed, for example to detect, type, and/or subtype an influenza infection. A sample suspected of containing a nucleic acid of an influenza virus, is screened for the presence or absence of that nucleic acid. The presence of the influenza virus nucleic acid indicates the presence of influenza virus. Determining whether the influenza virus nucleic acid is present in the sample can be accomplished by detecting hybridization between an influenza specific probe, influenza type specific probe, and/or subtype specific probe and an influenza nucleic acid. Probes and primers for the detection, typing and/or subtyping of influenza virus are also disclosed. Kits and arrays that contain the disclosed probes and/or primers also are disclosed. | 04-30-2009 |
20090111090 | METHOD FOR DETECTING INTEGRATED HPV DNA - A method for detecting integrated HPV DNA is described herein. This method comprises obtaining first and second samples, obtaining first and second information, and detecting, based on the first and second information, the HPV DNA integrated into the genome of a cell derived from a subject. The second sample comprises DNA derived from the cell, which is treated with an enzyme having exonuclease activity. The first information is related to the amount of HPV DNA in the first sample, and the second information is related to the amount of HPV DNA in the second sample. | 04-30-2009 |
20090111091 | SPECIMEN PRETREATMENT LIQUID, KIT FOR MEASURING VIRUS, AND METHOD FOR DETECTING VIRUS - The invention provides a pretreatment liquid for preparing a sample for measuring a virus included in rhinorrhea or sputum by an immunoassay method using an antibody specifically binding to the virus, comprising a protease inhibitor for inhibiting an influence of a protease to the virus, as well as a virus measurement kit and a virus detection method using the specimen pretreatment liquid. | 04-30-2009 |
20090111092 | DETERMINATION OF HEPATITIS C VIRUS GENOTYPE - The present invention provides compositions and methods for the detection and characterization of HCV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, to determine HCV genotype. | 04-30-2009 |
20090117536 | METHOD AND DEVICE FOR BACTERIAL SAMPLING - A bacterial detection sampling device comprising: a sampling medium for receiving a bacterial sample; and a plurality of bacteriophage. The bacteriophage are located on or in the sampling medium. Each bacteriophage comprises a nucleic acid encoding a protein capable of emitting light at an output wavelength. | 05-07-2009 |
20090117537 | METHOD FOR DETECTING SARS CORONAVIRUS - This invention provides: a method for detecting SARS pathogenic viruses with high sensitivity and rapidity for diagnosing severe acute respiratory syndrome (SARS); an oligonucleotide primer that can specifically hybridize with any nucleotide sequence constructed based on the nucleotide sequence of RNA polymerase of the SARS coronavirus; a method for nucleic acid amplification using such primer; a method for diagnosing infection with the SARS coronavirus via detection of nucleic acid amplification; and a kit for diagnosing SARS. | 05-07-2009 |
20090123909 | Rapid, Informative Diagnostic Assay For Influenza Viruses Including H5N1 - A rapid diagnostic assay for influenza virus, particularly avian influenza and more particularly H5N1, is described. The assay is based on amplification of a significant portion of the hemagglutinin (HA) gene and sequencing of several loci within the HA gene, using techniques which can obtain real time sequence information from multiple sites of a target DNA, in particular pyrosequencing and bioluminescence regenerative cycle. The assay contemplates the use of information-rich subsequences within the HA gene, e.g., (1) a glycosylation sequon; (2) receptor binding site; and (3) HA1/HA2 cleavage site. Other subsequences for sequencing include strain and clade markers, which vary among H5N1 strains. | 05-14-2009 |
20090123910 | Method of efficient extraction of protein from cells - Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided. | 05-14-2009 |
20090130649 | Methods for Genotyping HVC - The present invention relates to methods for differentiating Hepatitis C virus (HCV) group A genotypes from HCV group B genotypes. The invention finds application in determining prognosis, and in the selection of treatment regimes, for patients infected with HCV. | 05-21-2009 |
20090130650 | Methods for the production of highly sensitive and specific cell surface probes - A system and method for producing an oligonucleotide having a high affinity for extracellular or cell surface markers on a target cell. The resultant oligonucleotide probe can be used to detect a target biomolecule, in particular a cancer cell or infectious agent such as a bacterium, virus, or fungus, comprising an aptamer having a high affinity for the biomolecule, wherein at least one labeled dye is attached to the aptamer. The labeled dye causes the aptamer to emit a baseline, non-visible emission. When the aptamer (also referred to herein as a probe) of the invention interacts with a target biomolecule, the fluorescence emission changes from the baseline emission to an emission that is visually detectable. | 05-21-2009 |
20090130651 | VARIANTS OF HEPATITIS B VIRUS WITH RESISTANCE TO ANTI-VIRAL NUCLEOSIDE AGENTS AND APPLICATIONS THEREOF - The present invention relates generally to viral variants exhibiting reduced sensitivity to particular agents and/or reduced interactivity with immunological reagents. More particularly, the present invention is directed to hepatitis B virus (HBV) variants exhibiting complete or partial resistance to nucleoside or nucleotide analogs and/or reduced interactivity with antibodies to viral surface components including reduced sensitivity to these antibodies. The present invention further contemplates assays for detecting such viral variants, which assays are useful in monitoring anti-viral therapeutic regimens and in developing new or modified vaccines directed against viral agents and in particular HBV variants. The present invention also contemplates the use of the viral variants to screen for and/or develop or design agents capable of inhibiting infection, replication and/or release of the virus. | 05-21-2009 |
20090130652 | Optimization of West Nile Virus Antibodies - The invention relates to the production of binding molecules. In particular, the invention relates to methods for producing binding molecules having an improved functionality of interest. | 05-21-2009 |
20090130653 | Interference Control Panel for Evaluation of Analytical Assays for Samples Derived from Blood - The invention relates to quality control of analytical assays, particularly NAT assays of blood samples containing nucleic acids. A control panel containing quantified amounts of substances known to interfere with an analytical assay is used and compared with a reference sample in the analytical assay. A comparison of the assay results interference panel validates the assay and can serve as a periodic quality control check for the analytical assay as well as related methods and protocols. The use of the control panel of the invention can also determine whether interfering substances are present and establish under what conditions the analytical assay reliable. | 05-21-2009 |
20090130654 | METHOD FOR SCREENING HIV DRUG SENSITIVITY - A method for monitoring ARV resistance, to determine viral fitness, and to forecast possible drug failure utilizes two nucleic acid sequences. One nucleic acid includes a retroviral nucleic acid devoid of at least a majority of the sequence for one of the two long terminal repeat regions. A second nucleic acid, includes a retroviral nucleic acid sequence devoid of the sequences encoding an envelope gene and the second long terminal repeat region of the retrovirus. The method allows the rapid cloning of an amplicon into an HIV-1 genome vector through recombination/gap repair in organisms such as yeast. The vectors can be directly passed to a mammalian cell line which has been specifically engineered to produce replication competent HIV-1 particles. The susceptibility of an isolate to any of several ARVs, i.e. PRIs, NRTIs, NNRTIs, T20, as well as entry and integrase inhibitors in developmentlclinical trials, may be tested. | 05-21-2009 |
20090130655 | Detection method using nanoaggregate-embedded beads and system thereof - The invention discloses a detection method using nanoaggregate-embedded beads and system thereof, which are characterized in that the nanoaggregate of Raman dye and metal nanoparticles is coated by an inorganic oxide to form a nanoaggregate-embedded bead, and which is then conjugated with a probe molecule to form a sensor bead. The Raman spectra of the product formed by binding of the sensor bead and an analyte in a sample is detected for determining whether the analyte exists in the sample. In embodiment, the pH of the solution of metal nanoparticles is controlled to keep at 10, and the concentration of the Raman dye is controlled to keep between 1×10 | 05-21-2009 |
20090136914 | Identification and use of genes encoding holins and holin-like proteins in plants for the control of microbes and pests - This invention provides: 1) methods for the identification of broad-spectrum holins with a high level of nonenzymatic activity in membranes; 2) conditions required for maintaining and increasing the anti-microbial and anti-pest efficacy of holins in gene fusions; 3) a method for effective targeting of holins expressed in plants through use of a leader peptide to direct the holin protein to the plant apoplast and xylem; 4) methods for the control of bacterial and fungal diseases of plants and control of insect and nematode pests that attack plants by expression of gene fusions involving holins, C-terminal additions and leader peptides, and optionally, endolysins; 5) methods for increasing the shelf-life of cut flowers, and 6) transgenic plants useful for the production of novel antimicrobial proteins based upon holins. | 05-28-2009 |
20090136915 | Methods and compositions for determining altered susceptibility of HIV-1 to anti-HIV drugs - This invention relates, in part, to methods and compositions for determining altered susceptibility of a human immunodeficiency virus (“HIV”) to the non-nucleoside reverse transcriptase inhibitors (“NNRTIs”) efavirenz (“EFV”), nevirapine (“NVP”), and delavirdine (“DLV”), the nucleoside reverse transcriptase inhibitor AZT, and the integrase strand transfer inhibitors diketo acid 1, diketo acid 2, and L-870,810 by detecting the presence of a mutation or combinations of mutations in the gene encoding HIV reverse transcriptase that are associated with altered susceptibility to the anti-HIV drugs. | 05-28-2009 |
20090136916 | METHODS AND MICROARRAYS FOR DETECTING ENTERIC VIRUSES - The present invention relates to methods, microarrays and kits for detecting one or more human astrovirus serotypes in a sample (e.g., a fecal sample) from an individual. The method includes amplifying nucleic acid molecules of the sample with one or more primers, to thereby obtain an amplified nucleic acid product; contacting the amplified nucleic acid product with one or more serotype specific probes having a nucleic acid sequence that is specific for only one astrovirus serotype in the group of astroviruses being assessed, wherein the nucleic acid sequence includes between about 9 and 25 nucleic acid bases (e.g., SEQ ID NO: 5-24); and detecting the hybridization complex. The presence of hybridization complexes with a serotype specific probe indicates the presence of one or more specific astrovirus serotypes, and the absence of hybridization complexes with a serotype specific probe indicates the absence of the specific astrovirus serotype. Identification of the astrovirus serotypes allows for one to diagnose an individual infected with the serotype. The present invention further includes microarrays having any one of the astrovirus specific probe, or kits having microarrays and reagents for carrying out the assay. | 05-28-2009 |
20090136917 | Systems and methods for viral therapy - Diagnostic methods and compositions associated with viral therapy are provided. In particular, methods, compositions, and kits to measure markers and therapeutic indicator predictive of viral efficacy in antitumor therapy are provided. Therapeutic viruses and combinations and kits for use in the practicing the methods also are provided. | 05-28-2009 |
20090142747 | Development of Diagnostic Kit for the Detection of Chrysanthemum Virus B - The present invention provides a method for detection of | 06-04-2009 |
20090142748 | MICROPOROUS MATERIALS, METHODS OF MAKING, USING, AND ARTICLES THEREOF - Described herein are methods for separating one or more analytes present in a fluid sample. The methods involve passing the fluid through or into a microporous material, wherein the analytes are localized near the surface of the microporous material. Additional processing steps such as hybridization and amplification can be performed once the analyte is localized. In one method, once the analyte is localized, the analyte can be detected, counted, and correlated in order to determine the concentration of the analyte in the sample. In another method, the localized analyte is destabilized to make the localized analyte more accessible for chemical manipulation. Modified microporous materials and composite materials are also disclosed that can be used in any of the methods and articles described herein. The composite is composed of a microporous material and a pigment, wherein the pigment is incorporated in the microporous material. The pigments alter the optical properties of the microporous material, which enhances the detection of analyte once it is localized. Methods for making pigmented composites are also disclosed. In a further aspect, various kits and articles such as filtration devices containing any of the microporous materials described herein are provided. | 06-04-2009 |
20090148828 | VIRAL DETECTION LIPOSOMES AND METHOD - A method of generating pathogen detecting liposomes includes a step of providing molecular beacons with fluorescing components. The molecular beacons include either strands of RNA or DNA and the fluorescing components include an emitter and a quencher. The method further uses nanodroplet technology to encapsulate the molecular beacons within a lipid membrane. Subsequently, receptors are assembled in association with the membrane. | 06-11-2009 |
20090148829 | Methods For Rapid Identification Of Pathogens In Humans And Animals - The present invention provides methods of: identifying pathogens in biological samples from humans and animals, resolving a plurality of etiologic agents present in samples obtained from humans and animals, determining detailed genetic information about such pathogens or etiologic agents, and rapid detection and identification of bioagents from environmental, clinical or other samples. | 06-11-2009 |
20090148830 | IDENTIFICATION OF MICROORGANISMS CAUSING ACUTE RESPIRATORY TRACT INFECTIONS (ARI) - The present invention relates to a method for the detection of acute respiratory tract infection (ARI) comprising the simultaneous amplification of several target nucleotide sequences present in a biological sample by means of a primer mixture comprising at least one primer set from each one of the following gene regions: the F1 subunit of the fusion glycoprotein gene for RSV, the hemagglutininneuraminidase gene for PIV-1, the 5′ noncoding region of the PIV-3 fusion protein gene, 16 S rRNA sequence for | 06-11-2009 |
20090148831 | NS5A nucleotide sequence variation as a marker for interferon response - Methods and reagents for determining a nucleotide variation at position 937 of the HCV-1a NS5A gene useful in predicting an individual's response to interferon treatment are presented. | 06-11-2009 |
20090148832 | Compositions and Methods for Generation of Infectious Hepatitis C Virus in Immortalized Human Hepatocytes - The present invention provides a cell line capable producing infectious hepatitis C virus 1a (HCV 1a) particles in culture. Disclosed are compositions and methods for an HCV 1a (clone H77) transfected immortal human hepatocyte (IHH) capable of generating infectious HCV 1a virus particles in culture. Also disclosed are methods of using the cell line, or HCV 1a virus particles derived from said cell line, to screen for potential therapeutic agents which interfere with HCV 1a virus propagation to treat hepatic disease. | 06-11-2009 |
20090155768 | Reporter plasmid phage packaging system for detection of bacteria - The invention is related to a transducing particle that comprises a bacteriophage coat and a DNA core that comprises plasmid DNA comprising: a) a host-specific bacteriophage packaging site wherein the packaging site is substantially in isolation from sequences naturally occurring adjacent thereto in the bacteriophage genome, b) a reporter gene, c) a bacteria-specific promoter operably linked to said reporter gene, d) a bacteria-specific origin of replication, and optionally e) an antibiotic resistance gene. The invention includes phage transducing particles, methods of making transducing particles, and methods of using the transducing particles in bacterial detection. | 06-18-2009 |
20090155769 | DETECTION METHOD FOR LJUNGAN VIRUS - The present invention relates to a method for specifically detecting Ljungan virus (LV). In particular, the present invention relates to a method of detecting LV using quantitative real-time reverse transcriptase PCR. The present invention also provides kits for performing the method of the invention. | 06-18-2009 |
20090155770 | IMPLANTABLE DEVICES FOR FIBER OPTIC BASED DETECTION OF NOSOCOMIAL INFECTION - Disclosed are methods and devices for continuous in vivo monitoring of a potential infection site. Disclosed devices may be utilized to alert patients and/or health care providers to the presence of a pathogen at an early stage of a hospital acquired infection, thereby providing for earlier intervention and improved recovery rates from bacterial infection. Disclosed methods utilize implantable devices for location at an in vivo site. The implantable device is held in conjunction with an optical fiber that detects and transmits an optically detectable signal generated in the presence of a pathogen. Upon generation of the emission, the optically detectable emission signal may be transmitted to a portable detection/analysis device. Analysis of the characteristics of the emission signal produced may be used to determine the presence or concentration of pathogens at the site of inquiry, following which real time information may be transmitted to medical personnel, for instance via a wireless transmission system. | 06-18-2009 |
20090155771 | HEPATITIS B VIRUS DNA POLYMERASE AND SURFACE ANTIGEN VARIANTS AND METHODS OF USING SAME - The present invention relates generally to viral variants exhibiting reduced sensitivity to agents and in particular nucleoside analogues. More particularly, the present invention is directed to hepatitis B virus variants exhibiting complete or partial resistance to nucleoside analogues. The variants may also comprise corresponding mutations affecting immunological interactivity to viral surface components. The present invention further contemplates assays for detecting such viral variants which assays are useful in monitoring anti-viral therapeutic regimes and in developing new or modified vaccines directed against viral agents and in particular hepatitis B virus variants. The present invention also contemplates the use of the viral variants to screen for agents capable of inhibiting infection, replication and/or release of the virus. | 06-18-2009 |
20090155772 | Method for detecting nanbv associated seroconversion - The present invention relates to recombinant expression vectors which express segments of deoxyribonucleic acid that encode recombinant HIV and HCV antigens. These recombinant expression vectors are transformed into host cells and used in a method to express large quantities of these antigens. The invention also provides compositions containing certain of the isolated antigens, diagnostic systems containing these antigens and methods of assaying body fluids to detect the presence of antibodies against the antigens of the invention. | 06-18-2009 |
20090155773 | High-Risk Human Papillomavirus Detection - This invention provides compositions and methods for detecting HPV in a sample. This invention also provides related kits, systems, and computers. | 06-18-2009 |
20090155774 | Fluorescence resonance energy transfer screening assay for the identification of HIV-1 envelope glycoprotein-medicated cell - This invention provides: agents determined to be capable of specifically inhibiting the fusion of a macrophage-tropic primary isolate of HIV-1 to a CD4 | 06-18-2009 |
20090162831 | HUMAN PARVOVIRUS - The present invention relates to the discovery of a new human parvovirus, methods of detecting the parvovirus and diagnosing parvovirus infection, methods of treating or preventing parvovirus infection, and methods for identifying anti-parvoviral compounds. | 06-25-2009 |
20090162832 | Functional viral vectors for the overexpression or extinction of particular genes in plants, and applications - The invention relates to the use of genes which, in plants, encode proteins with a functional diversity in terms of silencing, comprising the selection of the gene with the level of effectiveness in order to construct a plant viral vector having the function of overexpressing or silencing particular genes. | 06-25-2009 |
20090162833 | TEST DEVICE FOR RAPID DIAGNOSTICS - Devices for detecting analytes or analogues thereof in a biological sample are disclosed. The device includes a solid support. The solid support has several juxtaposed zones. The sample is able to migrate from a sample receiving zone towards a detection zone. The analyte, if present, is detected in the detection zone. Both zones have material allowing a capillary flow of the sample through the zones. In between the zones, there is an intermediate zone of transport of the sample which is free from any capillary material. This allows the ample to migrate by gravitational forces on the support laid in a vertical position. Methods for detecting analytes or analogues thereof in a biological sample using the device are also disclosed. | 06-25-2009 |
20090162834 | MOLECULAR SEQUENCE OF SWINE RETROVIRUS AND METHODS OF USE - Purified nucleic acid which can specifically hybridize with the sequence of swine retroviruses. | 06-25-2009 |
20090170062 | Device and method for detecting analytes by visualization and separation of agglutination - The invention relates to a device for detecting one or several analytes in a sample, characterized in that it comprises one or more reaction chambers and/or one or more reagent application channels, and one or more capillary systems and one or more negative vessels. The invention also relates to a method for detecting one or more analytes in a sample fluid by visualization of agglutination, characterized in that a) the sample fluid is brought into contact with a reagent, b) the reaction mixture is exposed to the effects of gravitation or magnetism, wherein the reaction mixture is strained through the capillary system of the inventive device with a negative vessel connected to the inventive device, and c) the reaction between the analyte and the reagent is determined. The invention also relates to one such method wherein the reaction mixture is brought into contact with another reagent during step b). The invention further relates to a method wherein the order of the individual steps consisting of a) and b) are reversed, particularly when the sample fluid is brought into contact with a reagent only during the effects of gravitation or magnetism. | 07-02-2009 |
20090170063 | Hcv rna having novel sequence - A truncated form hepatitis C virus gene wherein part of the gene region encoding from the core protein to the NS2 protein of hepatitis C virus has been deleted while retaining the translation frame. In particular, the gene according to claim | 07-02-2009 |
20090170064 | ISOTHERMAL SCREENING OF HUMAN PAPILLOMAVIRUS RELATED NUCLEIC ACIDS - The presently described technology relates generally to the art of molecular diagnostics and more particularly to point-of-care diagnostic methods and materials. The diagnostic methods and materials of the presently described technology are suitable for a variety of uses including but not limited to the bedside or field diagnosis of infectious or noninfectious diseases. | 07-02-2009 |
20090170065 | METHOD FOR TRANSPORT OF MAGNETIC PARTICLES AND DEVICES THEREFOR - The present invention is related to a method for re-enabling transport by means of a magnetic field gradient transport mechanism of magnetic beads comprising a ligand in a solution on top of a surface comprising a receptor bound with said ligand, comprising the step of changing the properties of said solution such that dissociation occurs between said ligand and said receptor, and such that a sufficient repulsive interaction is created between said surface and said bead to allow transport of said bead. | 07-02-2009 |
20090170066 | METHODS AND MATERIALS THEREFOR - The present invention relates to a method for detecting or detecting and identifying rotavirus in a biological sample. In particular, the invention relates to a detection method comprising contacting the nucleic acids from the sample or derived from the sample with at least one VP4 and/or VP7 universal probes in the context of a solid support and detecting any type-specific hybridisation. The invention further relates to a detection or detection followed by typing method comprising contacting the nucleic acids from the sample or derived from the sample with at least one P type-specific and G type-specific probes in the context of a solid support and detecting any type-specific hybridisation. The invention also relates to primers and probes used therein and to diagnostic kits. | 07-02-2009 |
20090170067 | Simian tropic, recombinant human immunodeficiency-1 viruses - The present invention relates to a vector for producing recombinant human immunodeficiency virus 1 (HIV-1) that is capable of infecting simian cells and monkeys. The recombinant HIV-1 overcomes blocks to infection mediated by simian cell gene products. Such recombinant viruses are useful for evaluating the effectiveness of antiretroviral therapies and vaccines. | 07-02-2009 |
20090170068 | Molecular Identification Through Membrane-Engineered Cells - The present invention relates to the development of analytical devises based on one or more cells (cellular biosensors) the surface of which has been modified by the artificial insertion of molecules that can react specifically with analytes under determination. These receptor molecules may be proteins (such as enzymes, antigens or antibodies), nucleic acids, carbohydrates, lipids or belong to any other chemical group able to react specifically with target molecules (<>) under determination in one or more samples. The introduction of these molecules into the cell surface can be achieved by electroinsertion or any other appropriate method. Different types of molecules can be inserted into the surface of the same cell, particularly if this contributes to the selectivity of the reaction with the analytes under determination. The method includes the use of an appropriate biosensor containing the modified cellular material in free state or immobilized in a gel or on a substrate made of appropriate material, so that the measurement of the selective reaction with the analyte under determination is ensured. The measurement of the reaction can be achieved by any appropriated method related to a physical chemical property of the sensor, such as the measurement of the change of the electric potential or various optical properties (such as fluorescence, chemiluminescence or electrogenerated chemiluminescence). Consequently, the determination of a chemical or biological compound is possible provided that the pattern of a certain physical chemical property of the biosensor in response to various concentrations of this compound is known, relative to other compounds of similar structure or function. | 07-02-2009 |
20090170069 | Cell free methods for detecting protein-ligand binding - Provided are rapid and sensitive cell-free assay methods for detecting and/or measuring specific bimolecular or higher order interactions via reassembly of a split monomeric reporter protein, and methods of detecting or identifying modulators of such interactions by the effect on the signal provided by the reassembled split reporter protein. This methodology is adaptable to protein-protein, protein-peptide, protein-nucleic acid, protein-methylated or nonmethylated nucleic acid and other small or large molecule ligands and binding proteins. | 07-02-2009 |
20090170070 | INCREASED SPECIFICITY OF ANALYTE DETECTION BY MEASUREMENT OF BOUND AND UNBOUND LABELS - The present invention describes the provision of an internal control in analytical techniques involving labeling of analytes, such as SERRS, for detection of an analyte, particularly a biomolecule in a sample, with improved accuracy. | 07-02-2009 |
20090170071 | SERUM BIOMARKERS OF HEPATITIS B VIRUS INFECTED LIVER AND METHODS FOR DETECTION THEREOF - The invention provides a method for detecting the presence of altered serum proteins in an Hepatitis B Virus (HBV)-infected patient with liver inflammation, comprising: obtaining a sample of serum from the patient; subjecting the sample to protein gel electrophoresis to separate proteins contained therein; staining proteins separated on the electrophoresis gel with silver nitrate solution; scanning the images of stained proteins into an image analysis scanner to obtain gel images; comparing the gel images to control samples of electrophoresis gels prepared from serum of normal patient and serum of HBV-infected patient with liver inflammation to determine whether the sample of serum from the patient contains specific serum proteins. This invention also provides serum protein biomarkers for the diagnosis of patients with HBV infection and liver inflammation. | 07-02-2009 |
20090170072 | DEVICE FOR COLLECTION AND ASSAY OF ORAL FLUIDS - A device for collecting and transporting aqueous fluid from the oral cavity to a lateral chromatographic strip for test is disclosed. The lateral chromatographic strip is placed within and extend along a cavity defined in a housing. At least one inspection site to the lateral chromatographic strip is provided to enable inspection of selected sites on the lateral chromatographic strip for test results. A porous wick material protrudes from the housing to a collection site exterior of the housing at one end and communicates to the lateral chromatographic strip at the other end. The porous wick material has particulate construction, the particles adsorbing aqueous oral fluid to transport the fluid from the mouth to the lateral chromatographic strip without substantial absorption. The particles of the porous wick material are bound together to define a continuous interstitial volume for the flow of oral fluid to be transported and are treated to be hydrophilic to the adsorbed oral fluids. The porous wick material readily releases oral fluid to the lateral chromatographic strip. Prevention of reverse flow to the oral cavity from the lateral chromatographic strip naturally occurs due to the circuitous flow path of the porous wick material. A bite plate is coupled to the housing and insertable between the teeth of the patient to position the porous wick in the oral cavity for collecting the oral fluid. The bite plate is typically held in place by the occlusal force of the teeth, preferably the molars and/or the bicuspids, to position the porous wick in the buccal space. By observing the lateral chromatographic strip while the test device is in the mouth immediate test results are obtained. | 07-02-2009 |
20090176200 | Modified Human Hepatitis C Virus Genomic RNA That can be Autonomously Replicated - The present invention provides modified hepatitis C virus genomic RNA, comprising nucleotide sequences of genomic RNA portions of two or more types of hepatitis C viruses, which comprises a 5′ untranslated region, a core protein coding sequence, an E1 protein coding sequence, a p7 protein coding sequence, an E2 protein coding sequence, an NS2 protein coding sequence, an NS3 protein coding sequence, an NS4A protein coding sequence, an NS4B protein coding sequence, an NS5A protein coding sequence, an NS5B protein coding sequence, and a 3′ untranslated region, and which can be autonomously replicated. In particular, the present invention relates to modified hepatitis C virus genomic RNA, which can be autonomously replicated by substitution of the RNA sequence portion encoding NS3, NS4, NS5A, and NS5B proteins of hepatitis C virus genomic RNA with a partial RNA sequence encoding NS3, NS4, NS5A, and NS5B proteins of a JFH1 strain shown in SEQ ID NO: 1. | 07-09-2009 |
20090176201 | MICROBUBBLES FOR AFFINITY SEPARATION - The present invention relates to methods, compositions and kits for affinity isolation, affinity purification and affinity assay based on microbubbles coated with an affinity molecule. Particularly, the invention provides protein microbubbles coated with an affinity molecule. In addition, the invention provides glass microbubbles coated with an affinity molecule. Methods of using the microbubbles of the invention for isolating analytes and cells are specifically provided. | 07-09-2009 |
20090176202 | Methods of Detecting Inhibitors of VIF-Mediated APOBEC3G Degradation and HIV - The invention comprises methods and cell lines for assaying APOBEC3G degradation and methods for identifying inhibitors of APOBEC3G degradation. The invention also provides methods of identifying inhibitors of HIV infection. The methods of the invention are useful for identifying inhibitors of viral infection, in particular, the methods of the invention are useful for treating retroviral infection. | 07-09-2009 |
20090176203 | Mutant HSV, Materials and Methods for Generation of Mutant HSV - A method of generating a mutant Herpes Simplex Virus (HSV) is disclosed, wherein the generated HSV genome comprises nucleic acid encoding a nucleic acid sequence of interest, the method comprising the steps of: i. providing a nucleic acid vector comprising nucleic acid encoding first and second site specific recombination sequences and a nucleic acid encoding a nucleic acid sequence of interest between said site specific recombination sequences; ii. providing an HSV, the genome of which comprises third and fourth site specific recombination sequences; iii. contacting said nucleic acid vector of (i) with said HSV of (ii) together with one or more recombinase enzymes capable of catalysing site specific recombination between the site specific recombination sequences of said nucleic acid vector and said HSV; iv. identifying HSV containing the nucleic acid sequence of interest, wherein steps i-iii are conducted in a cell-free system. | 07-09-2009 |
20090176204 | Method for improved diagnosis of dysplasias - The present invention relates to a method for improved diagnosis of dysplasias based on simultaneous detection of INK4a gene products and at least one marker for cell proliferation. Particularly the present invention provides a method for discriminating dysplastic cells over-expressing INK4a gene products from cells over-expressing INK4a gene products without being dysplastic by detection of a marker suitable for characterising the proliferation properties of the respective cell. The characterisation of the proliferation properties may comprise the detection of a marker or a set of markers characteristic for active cell proliferation and/or a marker or a set of markers characteristic for retarded or ceased cell proliferation. The method presented herein thus enables for a specific diagnosis of dysplasias in histological and cytological specimens. | 07-09-2009 |
20090181363 | NON-INVASIVE DETECTION OF FISH VIRUSES BY REAL-TIME PCR - A real-time assay coupled with a non-invasive tissue sampling was developed for the detection and quantification of fish viruses. As a proof of principles, data were presented for the detection and quantification of infectious hypodermal necrosis virus (IHNV) in trout. The primers were designed for IHNV nucleocapsid (N), and surface glycoprotein (G) genes, and trout &bgr;-actin and elongation factor-l&agr; (EF-I &agr;) were used as internal control for the assay. The reaction conditions for the real-time RT-PCR were optimized using cDNA derived from IHNV-infected Epithelioma papulosum cyprinid (EPC) cells. Using both N- and G-gene primers, IHNV was successfully detected in liver, kidney, spleen, adipose tissue and pectoral fin samples of laboratory-challenged and wild samples. The dissociation curves with a single melting peak at expected temperature (85° C. for the N-gene and 86.5° C. for the G-gene) confirmed the specificity of the N- and G-gene amplicons. The IHNV N- and the G-gene expression levels in different tissues of laboratory challenged samples were in the order of spleen, liver, kidney, adipose tissue and pectoral fin, however in the field-collected samples the order of gene expression was liver, kidney, pectoral fin, adipose tissue, and spleen. The N- and G-gene expressions in spleen were found to be dramatically lower in the field-collected samples compared to the laboratory-challenged samples indicating a potential difference in the IHNV replication in the laboratory as opposed to field conditions. The real-time PCR assay was found to be rapid, highly sensitive, and reproducible. Based upon the ability to detect the virus in pectoral fins a non-invasive detection method for IHNV and other fish viruses is developed. Such a non-invasive tissue sampling coupled with real-time PCR assay is very valuable for large-scale virus screening of fish in aquaculture facilities as well as for epidemiological studies. | 07-16-2009 |
20090181364 | ZINC BINDING COMPOUNDS AND THEIR METHOD OF USE - The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of zinc ions in a sample. The metal chelating moiety of the zinc-binding compound is an analog of the well-known calcium chelator, BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid), wherein the chelating moiety has been modified from a tetraacetic acid moiety to a tri- di- or monoacetic moiety. This change in acetic acid groups on the metal chelating moiety results in the selective bindings of zinc ions in the presence of calcium ions, both of which are present in biological fluids and intracellular cytosolic fluid and organelles. | 07-16-2009 |
20090181365 | NOROVIRUS DETECTION REAGENT - The present invention provides a combination of oligonucleotides preferable for composing a gene testing reagent capable of detecting all subtypes of norovirus rapidly and with high sensitivity. More specifically, the present invention provides a detection method in which only norovirus is specifically amplified and an oligonucleotide that binds to a specific site of norovirus, by using a primer having a sequence that is homologous or complementary to a base sequence specific for norovirus and is located at a position subject to minimal mutation according to subtype. | 07-16-2009 |
20090186336 | Immuno-PCR method for detecting nasopharyngeal carcinoma markers and kit thereof - The present invention is related to an immuno-PCR method for detecting nasopharyngeal carcinoma (NPC) and kit thereof, especially related to an immuno-PCR method for detecting markers of early stage NPC and kit thereof. The present invention includes providing a substrate whereon protein markers immobilized; applying a patient's specimen to the substrate; adding a solution which has biotinylated anti-human IgA secondary antibody and incubating the solution; adding a solution with a linker and biotinylated target DNA; proceeding a polymerase chain reaction; and finally, detecting the target DNA fragments via electrophoresis. | 07-23-2009 |
20090186337 | N PROTEIN OF A VIRUS OF THE PARAMYXOVIRIDAE FAMILY-PROTEIN OF INTEREST FUSION PROTEINS - The invention relates to N protein-protein of interest fusion proteins, optionally in the form of soluble N protein-protein of interest/P protein complexes, the N and P proteins being proteins of a virus of the Paramyxoviridae family. When the protein of interest is an antigen, the invention relates also to vaccinal compositions and diagnostic reagents comprising those N protein-antigen fusion proteins or those N protein-antigen/P protein complexes. The N protein-protein of interest fusion protein can also be used as a “vector” for transporting into cells therapeutic molecules of interest, such as antivirals or anticancer agents. | 07-23-2009 |
20090186338 | OLIGONUCLEOTIDES ORIGINATING FROM SEQUENCES CODING FOR THE SURFACE COMPONENT OF PTLV ENVELOPE PROTEINS AND THEIR USES - The invention relates to the use of oligonucleotides from the nucleotide sequences coding for the amino-terminal region of the surface component (SU) of envelope proteins of PTLV viruses in order to perform methods of detecting every PTLV strain or PTLV-related viruses, e.g. for the detection of novel PTLV variants or viruses comprising sequences related to PTLV SUs. The invention also relates to primer pairs which are used to perform said detection methods and the novel PTLV variants thus detected. | 07-23-2009 |
20090191538 | Selective detection of oncogenic HPV - Compositions and methods for discriminately detecting the presence of a set of related genes from target organisms while avoiding detection of closely similar genes in non-target organisms. The present invention achieves this objective by a variety of novel nucleic acid constructs and methods. The nucleic acid constructs of the present invention are able to carry out this objective by virtue of the selected sequences of the compositions and by methods of use of such compositions. | 07-30-2009 |
20090191539 | Primers for isothermal amplification of hepatitis C virus - The present application relates to primers for isothermal amplification of HCV each include at least eighteen consecutive bases corresponding to a 3′ end region of one selected from base sequences of SEQ ID NOs: 1-10, 21 and 22. The primers are specific to HCV subtypes 1a, 1b, 2a, 2b and 3a, respectively and enable genotyping of HCV by isothermal amplification. | 07-30-2009 |
20090191540 | Markers for Viral Infections and Other Inflammatory Responses - Compositions and methods for the detection, diagnosis and treatment of BVDV are provided. | 07-30-2009 |
20090197243 | Method and composition for rapid viability testing of cells - The present invention relates to a method for rapidly monitoring a stress response of a cell to a stressor and determining the magnitude of the stress response; a method for rapidly detecting the presence or absence of a cell by monitoring a stress response of the cell if said cell is present, or the absence of the stress response if said cell is absent or dead; and a method for determining a predictive outcome for the susceptibility of a cell to a selected concentration of a bio-active agent or environmental factor and a level of stress of the cell at the selected concentration of the bio-active agent. Also disclosed are kits for carrying out the methodology according to an embodiment of the invention. | 08-06-2009 |
20090197244 | Method for typing and detecting HBV - The present invention relates to a method for detection and/or genetic analysis of HBV in a biological sample, comprising hybridizing the polynucleic acids of the sample with a combination of at least two nucleotide probes, with said combination hybridizing specifically to a mutant target sequence chosen from the HBV RT pol gene region and/or to a mutant target sequence chosen from the HBV preCore region and/or to a mutant target sequence chosen from the HBsAg region of HBV and/or to a HBV genotype-specific target sequence, with said target sequences being chosen from FIG. | 08-06-2009 |
20090197245 | Rapid detection of dengue virus - One example of a solution provided here comprises providing a single-stranded oligonucleotide, the oligonucleotide being complementary to a portion of SEQ ID NO:1, and contacting the oligonucleotide with a nucleic acid comprising the sequence of SEQ ID NO:1, under conditions that permit hybridization of the oligonucleotide with the nucleic acid. Another example comprises providing a single-stranded oligonucleotide comprising the sequence of SEQ ID NO:4, and contacting the oligonucleotide with a nucleic acid comprising the sequence of SEQ ID NO:1, under conditions that permit hybridization of the oligonucleotide with the nucleic acid. | 08-06-2009 |
20090202983 | Method For Determining The Concentration of Virus Particles/Virus Antigens - The invention provides a method for determining the concentration of virus particles and/or virus antigens in a sample. In particular, the invention relates to determining the concentration of influenza virus particles/influenza virus antigens in a sample. The invention further relates to the use of an ion-exchange matrix for the determination of the concentration of virus particles and/or virus antigens in a sample. | 08-13-2009 |
20090202984 | SINGLE MOLECULE NUCLEIC ACID SEQUENCE ANALYSIS PROCESSES AND COMPOSITIONS - Improved solid supports and methods for analyzing target nucleotide sequences are provided herein. Certain improvements are directed to efficiently preparing nucleic acids that comprise nucleotide sequences identical to or substantially identical to one or more target nucleotide sequences, or complement thereof. The prepared nucleic acids include a reference sequence that facilitates sequence analysis. The solid supports and methods provided herein minimize the number of steps required by published sequence analysis methodologies, and thereby offer improved sequence analysis efficiency. | 08-13-2009 |
20090202985 | METHOD AND APPARATUS FOR DETECTING AN ELECTRIC FIELD FLUCTUATION ASSOCIATED WITH THE PERMEABILIZATION OF A BACTERIAL CELL WALL - A sensor for detecting an electric field fluctuation associated with the permeabilization of a bacterial cell wall comprises a substrate, at least two electrodes integrated on the substrate, an amplifier integrated on the substrate, and a processor electrically connected to the amplifier to analyze the amplified signal. The substrate and the at least two electrodes define a well between the at least two electrodes, and the at least two electrodes being configured to generate a signal in response to an electric field fluctuation in close proximity to the well or the electrodes triggered when at least one antibacterial agent associated with the well contacts a cognate target. The amplifier is configured to generate an amplified signal in response to the signal. In addition, the processor is electrically connected to the amplifier to analyze the amplified signal. | 08-13-2009 |
20090208924 | Generation of Replication Competent Viruses for Therapeutic Use - The present invention relates to the generation or replication-competent viruses having therapeutic utility. The replication-competent viruses of the invention can express proteins useful in the treatment of disease. | 08-20-2009 |
20090208925 | Methods and Compositions for Determining Hypersusceptibility of HIV-1 to Non-Nucleoside Reverse Transcriptase Inhibitors - This invention relates to methods for determining hypersusceptibility of HIV-1 viruses to non-nucleoside reverse transcriptase inhibitors (NNRTIs) based on the viral genotypes. The methods generally comprise detecting, in a gene encoding reverse transcriptase of the HIV-1, the presence of a mutation at codon 65, 69, or 74 alone or in combination with one or more mutations at certain other codons. Combinations of mutation associated with hypersusceptibility to NNRTIs are also disclosed. | 08-20-2009 |
20090208926 | DIAGNOSIS OF LIVER PATHOLOGY THROUGH ASSESSMENT OF PROTEIN GLYCOSYLATION - Methods for diagnosing pathology of the liver in a subject suspected of having such pathology are disclosed. The methods comprise quantifiably detecting glycosylation, and more specifically fucosylation, on proteins in biological fluids, and comparing the detected glycosylation with reference values for the glycosylation of such proteins in healthy or disease states. | 08-20-2009 |
20090215027 | Method and system for colorimetric determination of a chemical or physical property of a turbid medium - A new method and a system for the simultaneous determination of a biological, chemical and/or physical property of a plurality of individual samples of a turbid is described. The invention relates to a system and colorimetric method for simultaneous determination and measuring properties, such as acidification or pH value, redox potentials, viscosity, diffusion, enzymatic activity, etc. of a plurality of individual samples of a turbid or opaque medium, such as, e.g. milk, whey and related products. In particular, this invention relates to a method for non-invasively and/or non-destructively scanning samples or an array of samples, and determine on the basis of the scanning a specific property, such as pH, of the samples. The method may also be used for multivariate determinations of chemical and/or physical properties. | 08-27-2009 |
20090215028 | Methods and Compositions for Determining Anti-HIV Drug Susceptibility and Replication Capacity of HIV - This invention relates, in part, to methods and compositions for determining the susceptibility of an HIV to an anti-HIV drug or the replication capacity of an HIV. In certain embodiments, the methods comprise culturing a host cell in the presence of the anti-HIV drug, measuring the activity of the indicator gene in the host cell; and comparing the activity of the indicator gene as measured with a reference activity of the indicator gene. In certain embodiments, the difference between the measured activity of the indicator gene relative to the reference activity correlates with the susceptibility of the HIV to the anti-HIV drug, thereby determining the susceptibility of the HIV to the anti-HIV drug. In certain embodiments, the difference between the measured activity of the indicator gene relative to the reference activity indicates the replication capacity of the HFV, thereby determining the replication capacity of the HIV. In certain embodiments, the host cell comprises a patient-derived segment and an indicator gene. In certain embodiments, the patient-derived segment comprises a nucleic acid sequence that encodes integrase or RNAse H. | 08-27-2009 |
20090215029 | METHODS OF ISOLATING AND PURIFYING NUCLEIC ACID-BINDING BIOMOLECULES AND COMPOSITIONS INCLUDING SAME - The invention provides methods for isolating or purifying a biomolecule directly or indirectly bound to a region of interest of a nucleic acid in a cell, and methods for isolating or purifying a biomolecule that directly or indirectly binds to a region of interest of a nucleic acid in a cell. The invention further provides substantially cell free, isolated and purified biomolecule(s) that are fixed or cross-linked to a region of interest of a nucleic acid, which optionally reflects the interaction of the biomolecule(s) with the nucleic acid in the cell (e.g., in native chromatin) when fixed or cross-linked to the region of interest. Substantially cell free, isolated and purified biomolecules that are fixed or cross-linked to a region of interest of a nucleic acid can remain fixed or cross-linked to the region of interest of the nucleic acid when heated or treated with denaturing compounds or agents. | 08-27-2009 |
20090220937 | Compositions for Use in Identification of Adventitious Viruses - The present invention provides compositions, kits and methods for rapid identification and quantification of adventitious contaminant viruses by molecular mass and base composition analysis. | 09-03-2009 |
20090220938 | VIRAL NUCLEOPROTEIN DETECTION USING AN ION CHANNEL SWITCH BIOSENSOR - The present invention provides a method of detecting viruses, such as respiratory-related viruses, in a sample with a sensitivity of at least 80%, and/or specificity of at least 90%, and/or with an accuracy of at least 90%. The method comprises contacting the sample with a biosensor. The present invention also provides a biosensor comprising a membrane and a solid conducting surface, with the membrane being attached to the solid conducting surface in a manner such that a reservoir exists therebetween. The membrane comprises first and second layers each comprising closely packed amphiphilic molecules; a plurality of first and second ionophores located in the first and second layers, respectively; and a plurality of antibodies or fragments thereof directed against nucleoproteins of respiratory-related viruses, more specifically, nucleoproteins of an influenza virus, and covalently attached to the second ionophores. The present invention further provides a device comprising an array of such biosensors. | 09-03-2009 |
20090220939 | Methods for determing resistance or susceptibility to hi entry inhibitors - The invention provides a method for determining whether a human immunodeficiency virus is likely to be more resistant to a viral entry inhibitor than a reference HIV. In certain aspects, the methods comprise comparing the length of one or more variable regions of an envelope protein of the HIV or a number of glycosylation sites on the envelope protein of the HIV to a length of one or more corresponding variable regions of an envelope protein of the reference HIV or a number of glycosylation sites on the envelope protein of the reference HIV, wherein the HIV is likely to be more resistant to the CD4 binding site entry inhibitor than the reference HIV when the HIV has longer variable regions than the reference HIV or the HIV has more glycosylation sites than the reference HIV. | 09-03-2009 |
20090220940 | Method for Testing the Integrity of Membranes - A method for evaluating the integrity of microfiltration, ultrafiltration and nanofiltration membranes, which method comprises passing a liquid that contains a substantially mono-dispersed population of nano-probes through said membrane to form a permeate and testing said permeate for the presence of said nano-probes, wherein the non-detection of said nano-probes in said permeate indicates that said membrane is substantially intact. | 09-03-2009 |
20090220941 | COMPOSITIONS FOR- DETECTING OF INFLUENZA VIRUSES AND KITS AND METHODS USING SAME - An isolated composition-of-matter comprising a sialic acid bound to a sialic acid binding domain of a polypeptide is provided. Uses thereof and kits comprising same are also provided. | 09-03-2009 |
20090220942 | ACTIVATED SPLIT-POLYPEPTIDES AND METHODS FOR THEIR PRODUCTION AND USE - The present invention relates to a method to produce activated split-polypeptide fragments that on reconstitution immediately forms an active protein. The method relate to real-time protein complementation. Also encompassed in the invention is a method to split and produce split-fluorescent proteins in an active state which produce a fluorescent signal immediately on reconstitution. The present application also provides methods to detect nucleic acids; non-nucleic acid analytes and nucleic acid hybridization in real-time using the novel activated split-polypeptide fragments of the invention. | 09-03-2009 |
20090220943 | HCV GENOTYPING AND PHENOTYPING - The present invention includes methods of genotyping and phenotyping HCV. In one embodiment, the methods of the invention can be used to determine whether a HCV isolate is resistant to an antiviral drug. The invention also includes primers for amplifying a HCV NS3 region and kits. | 09-03-2009 |
20090220944 | Method to measure and characterize microvesicles in the human body fluids - This disclosure provides a method to capture, detect, characterize and quantify human exosomes in small volumes of human body fluids by using a sandwich ELISA test. This method allows a full characterization of an exosome preparation, thus providing a tool to distinguish a disease-related condition from a healthy state, by the use of a non-invasive assay. In fact, this method may be useful in screening, diagnosis and prognosis of tumors, with a simple plasma sample. At the same time measurement of circulating exosomes may provide information on the level of tumor mass present in a patient. The method provided here is suitable to evaluate presence of some infectious and/or transmissible agents, such as viral proteins or prion proteins, within circulating exosomes. | 09-03-2009 |
20090220945 | HPV E6, E7 mRNA ASSAY AND METHODS OF USE THEREOF - Provided is an HPV E6, E7 mRNA assay, referenced herein as the “In Cell HPV Assay,” that is capable of sensitive and specific detection of normal cervical cells undergoing malignant transformation as well as abnormal cervical cells with pre-malignant or malignant lesions. The In Cell HPV Assay identifies HPV E6, E7 mRNA via in situ hybridization with oligonucleotides specific for HPV E6, E7 mRNA and quantitates the HPV E6, E7 mRNA via flow cytometry. The In Cell HPV Assay can be carried out in less than three hours directly from liquid-based cervical (“LBC”) cytology specimens. The In Cell HPV Assay provides an efficient and highly sensitive alternative to the Pap smear for determining abnormal cervical cytology. | 09-03-2009 |
20090220946 | ADENOVIRUS STATUS AS A PREDICTOR OF BODY COMPOSITION CHANGE, DISEASE STATUS, AND TREATMENT OUTCOMES - Infection with obesifying adenoviruses in animals and humans may be used to predict changes in body weight and disease status. More particularly, infection with certain adenoviruses, such as adenovirus type 36 (Ad-36) and adenovirus type 37 (Ad-37) may cause removal of the normal equilibrium factors that control fat cell metabolism and may make individuals more responsive than normal individuals to perturbations, which cause body composition change including weight gain or weight loss. | 09-03-2009 |
20090233266 | STRUCTURE OF THE HEPATITIS C VIRUS NS2 PROTEIN - The present invention provides a crystallized C-terminal domain of an NS2 protein of hepatitis C virus, methods of producing the same and methods of use thereof. The present invention also relates to structural elements of the C-terminal domain of hepatitis C virus NS2 protein, and methods of inhibiting hepatitis C virus infection, replication and/or pathogenesis, by interacting with the same. | 09-17-2009 |
20090233267 | Methylated Tat Polypeptides and Methods of Use Thereof - The present invention provides isolated methylated Tat peptides; and compositions comprising the peptides. The present invention further provides isolated antibodies specific for a Lys-51-methylated Tat polypeptide. Also provided are methods of identifying agents that inhibit Lys-51 methylation of a Tat polypeptide. The present invention further provides methods of treating an immunodeficiency virus infection in a mammalian subject. | 09-17-2009 |
20090233268 | DETECTION OF BIOMARKERS AND BIOMARKER COMPLEXES - The invention features methods and devices for the detection of biomarker complexes and their components and for the sequential detection of multiple epitopes of a biomarker. The invention also features methods for diagnosing disease and evaluating the efficacy of treatment of a subject with a disease. | 09-17-2009 |
20090233269 | Detection of a Target in a Preservative Solution - This invention relates to methods, articles and compositions useful in detecting target substances in an alcoholic preservative solution, and for identifying sensors useful for binding to such targets. The methods allow for the simultaneous performance of sufficient fixation of a sample and binding of a detectable sensor to a target of interest in the sample. In one aspect, a method is provided that comprises contacting a sample suspected of containing a target with a detectable sensor molecule known to bind to such target in an alcoholic preservative solution. The method maybe performed in multiplex form to permit simultaneous analysis of a plurality of targets. Methods for identifying a sensor capable of binding to a desired target in an alcoholic preservative solution are also provided. An alcoholic preservative solution comprising one or more such detectable sensors is also provided. Also provided is a sample comprising a bound sensor provided by such a process. Also provided are kits useful for such methods. | 09-17-2009 |
20090246751 | METHOD FOR THE DETECTION OF VIRUS INFECTED CELLS - The present invention relates to a method for determining one or more stressed cell(s) in a medium. The method comprises the steps of (i) providing a sample from the medium, and (ii) determining a change in the sample relative to a normal sample of non-stressed cell(s). | 10-01-2009 |
20090246752 | APPARATUS AND METHOD FOR DETECTING MICROSCOPIC LIVING ORGANISMS USING BACTERIOPHAGE - A method for detecting one or more target bacteria in a raw sample where: 1) bacteriophage(s) specific to each target bacterium are added to the raw sample, 2) the test sample is incubated, and 3) the test sample is tested for the presence of each phage in sufficient numbers to indicate the presence of the associated target bacteria in the raw sample. In one embodiment, each phage is initially added to the raw sample in concentrations below the detection limit of the final phage detection process. In another embodiment, the parent phages are tagged in such a way that they can be separated from the progeny phage prior to the detection process. Preferred phage detection processes are immunoassay methods utilizing antibodies that bind specifically to each phage. Antibodies can be used that bind to the protein capsid of the phage. Alternatively, the phage can by dissociated after the incubation process and the sample tested for the presence of individual capsid proteins or phage nucleic acids. The invention can be used to test target bacteria for antibiotic resistance. | 10-01-2009 |
20090246753 | Detection of Phage Amplification by SERS Nanoparticles - A phage specific antibody presenting particle, devices and methods related to detection of phage amplification are provided. | 10-01-2009 |
20090246754 | OPTIMIZED PROBES AND PRIMERS AND METHODS OF USING SAME FOR THE DETECTION AND QUANTITATION OF BK VIRUS - Described herein are probes and primers for detecting and quantitating variant BK viral strains and methods and devices of identifying and using the described probes and primers. | 10-01-2009 |
20090253119 | LATERAL FLOW SYSTEM AND ASSAY - The present invention relates to a lateral flow assay and system, including a test strip, for detection and quantification of analytes in samples, such as samples containing cells and fluid. In general, according to the present invention, a test strip for a lateral flow assay for detection of at least one analyte in a sample comprises: (1) a chromatographic strip, a sample filter, a fluid-impermeable barrier, and means for providing a mobilizable detectable agent that is capable of binding to the at least one analyte or to the capture agent after capturing the analyte to the chromatographic strip such that the mobilizable detectable agent migrates through the chromatographic strip and contacts sample that has passed through the sample filter and also has migrated through the chromatographic strip. The test strip allows detection with or without quantitation of an analyte in a sample containing whole cells. | 10-08-2009 |
20090253120 | DNA VIRUS DETECTION BY DNA CHIP - The disclosure relates to chips containing nucleic acid probes or primers and their use in methods to detect nucleic acid molecules of DNA viruses. The disclosure includes DNA chips in contact with a thermocycler capable of automatically regulating the temperature, temperature cycle times, and number of temperature cycles of the chips to provide genetic diagnosis in one step. | 10-08-2009 |
20090253121 | METHOD FOR AMT-RFLP DNA FINGERPRINTING - An improved method for identifying and classifying organisms is provided. The method comprises the steps of obtaining a sample of DNA, isolating a portion of the sample to be analyzed, cutting the sample portion into at least two fragments, ligating both ends of the fragments with double-stranded linker sequences, adding the fragments to a polymerase chain reaction that amplifies a diagnostic region using a SYBR green-intercalating dye, and analyzing the fragments using a melt-curve analysis. | 10-08-2009 |
20090253122 | Method for measuring resistance of a patient HIV-2 to protease inhibitors - A search method in a biological sample containing an HIV 2 viral strain for possible resistance of said strain to treatment by an anti-protease agent, and nucleotide probes for the implementation thereof. According to methods known per se, the presence of at least one mutation at certain, specified, particular positions of the proteinic sequence of the protease of said viral strain from a biological sample taken from a patient contaminated by HIV 2 is searched. If said mutation is observed, the existence of a resistance to said anti-protease agent is assumed in the patient. | 10-08-2009 |
20090253123 | Hepatitis B variants with reduced sensitivity to therapeutic compounds, their detection and uses thereof - The present invention is a diagnostic kit and materials for: 1) the prediction of the long-term response of a chronic hepatitis B virus (HBV) carrier to treatment with nucleoside/nucleotide analogue, or their combination; 2) the detection of HBV variants that exhibit reduced reactivity to antibody detection; 3) the detection of HBV variants in the precore/core region that negatively affect the course of liver disease; 4) the identification of the HBV genotype. | 10-08-2009 |
20090253124 | METHOD FOR DETECTING HUMAN PAPILLOMAVIRUS mRNA - An in vitro method is provided for screening human female subjects to assess their risk of developing cervical carcinoma which comprises screening the subject for expression of mRNA transcripts from the E6 and optionally the L1 gene of human papillomavirus, wherein subjects positive for expression of L1 and/or E6 mRNA are scored as being at risk of developing cervical carcinoma. Kits for carrying out such methods are also provided. | 10-08-2009 |
20090258339 | SYSTEMS, METHODS AND COMPOSITIONS FOR DETECTION OF HUMAN PAPILLOMA VIRUS IN BIOLOGICAL SAMPLES - The present invention comprises, without limitation, systems, methods, and compositions for the detection, identification, and quantification, down to the single copy level, of human papillomavirus (HPV) in biological samples, including but not limited to, mammalian body fluids and cervix scrapings, for purposes of detection, treatment and/or management of cancer and dysplasia. | 10-15-2009 |
20090258340 | Assay for SARS coronavirus by amplification and detection of the replicase sequence - Primers and probes derived from SARS-CoV nucleic acid that facilitate detection and/or quantification of the replicase gene are disclosed. The disclosed sequences may be used in a variety of amplification and non-amplification formats for detection of SARS-CoV infection. | 10-15-2009 |
20090258341 | Compositions and Methods for Detecting Bacteria - Genetically modified bacteriophage and methods of using the same to detect bacterial types of interest are provided. | 10-15-2009 |
20090258342 | OPTIMIZED PROBES AND PRIMERS AND METHODS OF USING SAME FOR THE DETECTION, QUANTIFICATION AND GROUPING OF HIV-1 - Described herein are optimized primers and probes useful for detecting, quantitating and grouping variant HIV-1 strains, and methods and kits of using the described primers and probes. | 10-15-2009 |
20090258343 | Semi-quantitative immunochromatographic device and method for the determination of HIV/AIDS immune-status via measurement of soluble CD40 Ligand/CD154, A CD4+ T cell equivalent and the simultaneous detection of HIV infection via HIV antibody detection - A semi-quantitative, immunochromatographic dual test device for the simultaneous detection of HIV/AIDS immune status CD4+ T cell equivalents, such as soluble CD40 ligand/CD 154, and the detection of an HIV antibody, includes one or more support materials capable of providing lateral flow. The one or more support materials include at least one sample receiving area for receiving a biological sample containing a first target analyte, the first target analyte being a CD4+ T cell equivalent, such as soluble CD40 ligand/CD 154, and a second target analyte, the second target analyte being an HIV antibody. A second area, situated on the one or more support materials, has a movably contained detector ligand and or detector antigens, wherein the detector ligand and or detector antigens is capable of forming a mobile complex with the soluble CD40 ligand/CD 154 and or HIV antibodies, and at least a first capture area having a predetermined amount of a first immobile capture reagent, the first immobile capture reagent capable of specifically binding to the mobile complex formed by the soluble CD40 ligand/CD 154 protein and the detector ligand and providing a visible signal. The one or more support materials further have situated thereon at least a second capture area having a predetermined amount of a second immobile capture reagent that is capable of specifically binding to HIV antibodies present in the biological sample and providing a visible signal. | 10-15-2009 |
20090263785 | Modified HIV-1 Peptides and Use Thereof in the Detection of Anit-HIV Antibodies - The invention relates to synthetic peptides having sequence (III), which are derived from HIV-1 virus gp41 and which can be used in immunoassays for the detection of infections caused by HIV-1 viruses. The invention also relates to the method of preparing said peptides, to compositions and kits containing same and to the use thereof for diagnostic purposes. The invention further relates to immunoassays which use said peptides for the detection of anti-HIV virus antibodies. | 10-22-2009 |
20090263786 | Mass spectrometric analysis method - Methods of using mass spectrometry and in particular matrix assisted laser Ligand-Complex from Target and desorption-ionization (MALDI) mass spectrometry to analyze, or otherwise detect the presence of or determine the identity of intact ions of undigested, unfragmented covalently stabilized supramolecular target-ligand-complexes, as well as the use of these methods in various biological application such as characterization of antibodies, drug discovery, and complexomics including automated or higher throughput applications. | 10-22-2009 |
20090263787 | METHOD FOR SCREENING OF INFECTIOUS AGENTS IN BLOOD - This invention discloses using SPR technology to simultaneously and qualitatively detect the presence of infectious agent related antibodies and/or antigens in a serum sample, which can be used to screen for infectious agents in blood. It also discloses an efficient formula to make a mixed SAM that can greatly enhance the immobilization ability of the metal surface in SPR based techniques, which is good for the immobilization of relevant antigens and antibodies used for the screening of infectious agents in blood. | 10-22-2009 |
20090263788 | EFFICIENT ALGORITHM FOR PCR TESTING OF BLOOD SAMPLES - Systems, processes, and devices are provided which are useful for testing blood or plasma donations to detect those specific donations which are contaminated by a virus above a predetermined level. Samples are formed into pools which are subsequently tested for virus contamination by a high-sensitivity test such as PCR. The pools are tested in accordance with an algorithm by which a sample from each donation is mapped to each element of an N-dimensional matrix or grid. Each element of the matrix is identified by a matrix identifier, X | 10-22-2009 |
20090269732 | Methods for Diagnosing Oncogenic Human Papillomavirus (HPV) - Methods for diagnosis of HPV infection in a subject are provided. HPV infection in a subject can be determined by generating mass profile data for a biological sample from the subject and correlating the mass profile data with reference mass profiles to detect the presence or absence, and/or quantity of at least one biomarker associated with HPV infection. Methods for detecting at least one biomarker associated with HPV infection in a biological sample are also provided. | 10-29-2009 |
20090269733 | Devices and Methods for the Rapid Analysis of Pathogens in Biological Fluids - The present invention relates to devices and methods for rapidly determining whether a biological fluid contains a suspect Gram positive bacterial, a Gram negative bacterial or a viral pathogen. The invention particularly pertains to such devices and methods wherein the biological fluid is cerebrospinal fluid, and wherein the suspect pathogen is a causative agent of meningitis. | 10-29-2009 |
20090269734 | METHOD FOR DETERMINATION OF RECOGNITION SPECIFICITY OF VIRUS FOR RECEPTOR SUGAR CHAIN - A method in which the recognition specificity of a virus for a receptor sugar chain can be easily determined with a simple instrument or apparatus is provided. In a method for determining the recognition specificity of a virus for a receptor sugar chain or for determining the change in a host infected in accordance with the mutation of virus comprising, a sample of the virus is brought into contact with a support having a polymer with sialo-oligosaccharide immobilized on the surface thereof; and the degree of binding therein is assayed to determine the recognition specificity of said virus for said receptor sugar chain and to determine the change in a host range. The method is suitable for the surveillance of virus. | 10-29-2009 |
20090269735 | SAMPLE PRETREATMENT SOLUTION FOR IMMUNOLOGICAL TEST AND METHOD FOR USING THE SAME - Sample pretreatment solutions for influenza virus tests by immunochromatography are described. | 10-29-2009 |
20090275013 | Method for preparing a monoclonal antibody to the common epitope of nonstructural NSs protein of watermelon silver mottle virus and assay for detection of serological related species in the genus tospovirus with the monoclonal antibody - The invention is an assay for detection of Watermelon silver mottle virus (WSMoV)-serogroup tospoviruses using a monoclonal antibody and a method for preparing the monoclonal antibody. A hybridoma cell line that produces a monoclonal antibody against the NSs proteins of WSMoV-serogroup tospoviruses was produced. The hybridoma cell line produces a monoclonal antibody binding with peptide SEQ ID No. 19. | 11-05-2009 |
20090275014 | THERMAL CYCLING METHOD - This invention provides a method for carrying out nucleic acid amplification reactions involving heating and cooling of samples in sample vessels utilizing a heat block comprising a liquid. The method can be used to perform multiple nucleic acid amplification reactions simultaneously in which each of the reactions is performed so as to have temperature profiles. The apparatus can be used for performing PCR, and real time PCR in particular, with control and uniformity. | 11-05-2009 |
20090275015 | NON-INVASIVE RESPIRATORY RAPID DIAGNOSIS METHOD - A non-invasive method for rapidly determining the specific presence or absence of respiratory pathogens and chemical entities. | 11-05-2009 |
20090275016 | ARRAYED DETECTOR SYSTEM FOR MEASUREMENT OF INFLUENZA IMMUNE RESPONSE - A sensor chip for detecting an immune response against an influenza virus, the sensor chip including a substrate having a surface and a plurality of hemagglutinin polypeptides bound to discrete locations on the surface of the substrate, each hemagglutinin polypeptide having a hemagglutinin epitope. Detection devices containing the sensor chip and methods of detecting influenza immune responses are also described herein. | 11-05-2009 |
20090280471 | METHODS FOR RAPID IDENTIFICATION OF PATHOGENS IN HUMANS AND ANIMALS - The present invention provides methods of: identifying pathogens in biological samples from humans and animals, resolving a plurality of etiologic agents present in samples obtained from humans and animals, determining detailed genetic information about such pathogens or etiologic agents, and rapid detection and identification of bioagents from environmental, clinical or other samples. | 11-12-2009 |
20090280472 | Method for Detection of Antigens - The field of the invention relates generally to the detection of antigens, including, but not limited to, quantum dots (Qdots) and metal oxide nanoparticles. More specifically, the invention relates to the detection of antigens on a surface or in a source, which antigens include bacteria, viruses, and small proteins. In some embodiments, the invention can be used to detect biological warfare agents, such as anthrax and ricin. In some embodiments, the invention can be used for early detection of diseases in human and animals. The invention may utilize a swab-test and may further utilize a filtration process, such as with a syringe-disc. | 11-12-2009 |
20090280473 | Inhibition of membrane fusion proteins - Methods of inhibiting viral infection of a eukaryotic cell by a target virus having a class II virus fusion protein are provided. Also provided are methods of screening a test compound for the ability to inhibit infection by a virus having a class II viral fusion protein. Additionally provided herewith are aqueous-soluble proteins comprising a portion of a class II viral fusion protein comprising a Domain III of the viral fusion protein. | 11-12-2009 |
20090280474 | METHOD FOR DETECTING A VIRUS - This invention is related a method for increasing the sensitivity of detecting a viral target in a sample. The sensitivity may be increased by disrupting a complex comprising the target or by measuring the level of the target from a larger volume of the sample. | 11-12-2009 |
20090286222 | Mixed Cell Diagnostic Systems For Detection Of Respiratory, Herpes And Enteric Viruses - The present invention generally relates to the field of diagnostic microbiology, and, more particularly, to compositions and methods for detecting and differentiating one or more viruses or other intracellular parasites present in a specimen. The present invention also provides compositions and methods to evaluate the susceptibility of organisms to antimicrobial agents. | 11-19-2009 |
20090286223 | Methods And Kits For In Situ Measurement of Enzyme Activity and Amount Using Single Measurement System - Disclosed are methods and kits for measuring in situ the activity and amount of an enzyme in a sample using a single measurement system. The method includes the steps of: (a) contacting a sample to a capturing agent having the capacity to bind to the enzyme to be analyzed and immobilized on a solid matrix; (b) measuring the activity of the enzyme captured by the capturing agent; (c) contacting a detection antibody specific to the enzyme captured by the capturing agent; and (d) detecting an antigen-antibody complex formed in the step (c). There is no need for separate measurement system to measure the activity and amount of an enzyme, as well as the above methods and kits permit to measure the precise activity and amount of an enzyme simultaneously because the measurement of enzyme activity and amount is carried out in a single and same measurement system as to the same sample in almost simultaneous manner. | 11-19-2009 |
20090286224 | Method for producing human antibodies with properties of agonist, antagonist, or inverse agonist - A method for obtaining agonist, antagonist and inverse agonist, to a given physiological receptor is disclosed. For the method, use is made of in silico design synthetic immunogens, which are caused to act in vitro on human lymphocyte-containing cell populations. A preferred receptor is human CD152, particularly the regions of CDR1, CDR2 and CDR3 that elicit antibodies serving as antagonist, inverse agonist and agonist, respectively. Also provided is a method in the treatment of human peripheral lymphocytes for use in the screening of CD152 ligands that yield pharmacological effects. | 11-19-2009 |
20090286225 | METHOD AND APPARATUS FOR BACTERIOPHAGE-BASED DIAGNOSTIC ASSAYS - Bacteriophage are combined with a test sample in an incubator, and the bacteriophage-exposed test sample is conjugated and applied to a sample pad in contact with a lateral flow strip to determine the presence or absence of a target bacterium. The conjugation may be performed in the sample pad or prior to application of the bacteriophage-exposed test sample to the pad. The incubator comprises a bacteriophage container and an incubation container separated by a valve. The test sample may be inserted into the incubation chamber using a swab or a rod with a piercing tip and a sample collection eye. The valve comprises a breakable stem. An antibiotic may be added to the test sample to determine the antibiotic resistance or susceptibility of the bacterium. | 11-19-2009 |
20090286226 | Simple membrane assay method and kit - A simple membrane assay method for detecting or quantitating an analyte in a specimen sample using an assay device equipped with a membrane bound with a capture-substance to capture the analyte, including the steps of filtering a specimen sample using a filter, dropping the filtrate onto said membrane and detecting the presence of the analyte in said specimen sample, as well as a simple membrane assay kit for detecting the presence of an analyte in a specimen sample, including (1) a filter tube, and (2) an assay device equipped with a membrane bound with a capture-substance to capture the analyte. The method or the kit can decrease the occurrence of false positivity and can provide a highly accurate detection of the analyte such as pathogen and antibody in a specimen collected in a medical scene or by an individual. | 11-19-2009 |
20090286227 | Methods, Devices, Kits and Compositions for Detecting Whipworm - Methods, devices, kits and compositions for detecting the presence or absence of whipworm in a fecal sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of whipworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, roundworm, and heartworm. Confirmation of the presence or absence of whipworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated. | 11-19-2009 |
20090286228 | Methods, Devices, Kits and Compositions for Detecting Roundworm - Methods, devices, kits and compositions for detecting the presence or absence of roundworm in a mammalian sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm. Confirmation of the presence or absence of roundworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated. | 11-19-2009 |
20090286229 | Methods, Devices, Kits and Compositions for Detecting Roundworm - Methods, devices, kits and compositions for detecting the presence or absence of roundworm in a fecal sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm. Confirmation of the presence or absence of roundworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated. | 11-19-2009 |
20090286230 | Methods, Devices, Kits and Compositions for Detecting Roundworm - Methods, devices, kits and compositions for detecting the presence or absence of roundworm in a fecal sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm. Confirmation of the presence or absence of roundworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated. | 11-19-2009 |
20090286231 | Methods, Devices, Kits and Compositions for Detecting Roundworm, Whipworm, and Hookworm - Methods, devices, kits and compositions for detecting the presence or absence of one or more helminthic coproantigens in a sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm, whipworm and/or hookworm in a fecal sample from a mammal and may also be able to distinguish between one or more helminth infections. Confirmation of the presence or absence of roundworm, whipworm and/or hookworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated. | 11-19-2009 |
20090286232 | METHOD AND APPARATUS FOR ENHANCED SENSITIVITY IN BACTERIOPHAGE-BASED DIAGNOSTIC ASSAYS - A method of determining the presence or absence of a target microorganism in a sample to be tested, the method comprising: (a) combining with the sample an amount of bacteriophage capable of attaching to the target microorganism to create a bacteriophage-exposed sample; (b) providing conditions to the bacteriophage-exposed sample sufficient to allow the bacteriophage to attach to the target microorganism while inhibiting phage replication in a potentially cross-reactive, non-target microorganism; and (c) assaying the bacteriophage-exposed sample to detect the presence or absence of a bacteriophage marker to determine the presence or absence of the target microorganism; wherein the amount of the bacteriophage is between 10% to 70% of the threshold number of bacteriophage that the assay can detect, or between 1×10 | 11-19-2009 |
20090291428 | COMPOSITIONS AND METHODS FOR THE DETECTION AND TREATMENT OF POXVIRAL INFECTIONS - The invention encompasses an antibody that binds to and substantially inhibits the activity of at least one poxvirus complement inhibitor. Additionally, the application encompasses methods of detecting a poxvirus complement inhibitor and methods of decreasing the activity of a poxvirus complement inhibitor. | 11-26-2009 |
20090291429 | SUBSTANCES CAUSING DIFFERENTIATION - A DNA construct is described which contains a fusion gene under the control of a promoter. The fusion gene comprises at least one resistance gene and at least one reporter gene and is slightly toxic to a host cell transfected with that DNA construct. That DNA construct can be encoded on a plasmid or a virus. Further, a method is described for using the DNA construct to identify substances that may cause a differentiation in eukaryotic cells. | 11-26-2009 |
20090291430 | Electrophoretic Interactive Spectral Methods and Devices for the Detection and/or Characterization of Biological Particles - Methods for identifying a biological particle in a sample medium include generating an Electrophoretic Quasi-elastic Light Scattering (EQELS) spectrum for the biological particle in the sample medium. The EQELS spectrum is compared to a reference database comprising a plurality of spectra, and each of the plurality of spectra correspond to an EQELS spectrum for one of a plurality of known biological particles. The biological particle in the sample medium is identified from the comparison. | 11-26-2009 |
20090298048 | NON-FLUORESCENT, NON-ENZYMATIC, CHEMILUMINESCENT AQUEOUS ASSAY - This invention provides for nonfluorescent, nonenzymatic, chemiluminescent aqueous assays in which the binding of two ligands is determined by a water soluble label system that emits light upon contact with a chemical energy transferring composition. | 12-03-2009 |
20090298049 | METHODS FOR SAMPLE TRACKING - A method and apparatus are provided for identifying a biological sample obtained during either paternity screening, genetic screening, prenatal diagnosis, presymptomatic diagnosis, diagnosis to detect the presence of a target microorganism carrier detection analysis, forensic chemical analysis, or diagnosis of a subject to determine whether a subject is afflicted with a particular disease or disorder, or is at risk of developing a particular disorder, wherein the result obtained from the analysis is associated with the unique DNA fingerprint biological barcode of the genotype of the subject being analyzed. The methods and apparatus of the invention have application in the fields of diagnostic medicine, disease diagnosis in animals and plants, identification of genetically inherited diseases in humans, family relationship analysis, forensic analysis, and microbial typing. | 12-03-2009 |
20090298050 | IMMUNOCHROMATOGRAPHIC DEVICE - The present invention provides an immunochromatographic device, which contains the following (a) and (b): (a) a first device part holding a first insoluble carrier used for developing a complex formed with an analyte and a labeling substance comprising a metal labeled with a first binding substance that can bind to the analyte and capturing the analyte and the labeling substance at a reaction portion containing a second binding substance that can bind to the analyte, and (b) a second device part holding a second insoluble carrier used for developing a liquid and a third insoluble carrier used for absorbing a liquid, in such a way that the first insoluble carrier does not come into contact with the second insoluble carrier and the third insoluble carrier. | 12-03-2009 |
20090298051 | Test Kit and Method for Detecting Bacteriophage - Phages can be detected as rapid indicators of the hygienic quality of a sample. Both continuous flow methods and devices, single sample methods and devices, of various volumes, can be used. Single samples may be tested by single or multi-step testing methods. Test kits can be provided in easy-to-use formats. Certain phages, such as coliphage, are useful as indicators of fecal contamination. | 12-03-2009 |
20090305226 | Biomolecular Recognition of Crystal Defects - Discrete and diffuse defects in a surface are detected. Discrete defects that may compromise the performance may be repaired. | 12-10-2009 |
20090305227 | HIV-1 LATENCY MODEL FOR HIGH THROUGHPUT SCREENING - Isolated, latently infected T cell lines are provided that can be utilized in high throughput screening to discover compounds capable of activating HIV-I. The T cell lines harbor a latent HIV-I derived vector pro virus, which upon activation expresses a marker for late viral gene expression due to the insertion of the marker gene in the position of HIV-I envelope. | 12-10-2009 |
20090305228 | Potentiation of Apoptosis by Monoclonal Antibodies - The present invention relates to the use of an antibodies composition, the fucose content of which is less than 65%, for indicating apoptosis in vitro. | 12-10-2009 |
20090305229 | MULTIPLEX DETECTION OF RESPIRATORY PATHOGENS - Described are kits and methods useful for detection of respiratory pathogens (influenza A (including subtyping capability for H1, H3, H5 and H7 subtypes) influenza B, parainfluenza (type 2), respiratory syncytial virus, and adenovirus) in a sample. Genomic sequence information from the respiratory pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample. | 12-10-2009 |
20090305230 | Real Time Detection of Molecules, Cells and Particles Using Photonic Bandgap Structures - Provided herein is a photonic bandgap (PBG) detector effective to detect inorganic molecules, organic biomolecules or biopolymers, cells, subcellular organelles, and particles. The PBG detector utilizes photonic crystals having a binding agent attached to channel surfaces comprising the crystals to selectively bind a molecule, cell or particle of interest so that an increase in light transmission is detectably induced within the photonic bandgap upon binding. Also provided are methods of optically detectiing an analyte and of identifying the presence of a cell or a particle in a biological sample. | 12-10-2009 |
20090305231 | Sensitive Immunoassays Using Coated Nanoparticles - Coated nanoparticles comprising a core surrounded by a shell that increases the reflectance of the nanoparticle, wherein the coated nanoparticle does not include a Raman-active molecule, are provided. Test devices and immunoassay methods utilizing the coated nanoparticles are provided. | 12-10-2009 |
20090305232 | Peptides for Detection of Antibody to Porcine Reproductive Respiratory Syndrome Virus - The invention provides compositions and methods for the detection and quantification of PRRSV antibodies and antibody fragments using polypeptides. | 12-10-2009 |
20090311664 | Method for Detection of Cancer Cells Using Virus - The invention relates to compositions and methods for cancer cell detection in bodily samples wherein a cancer cell can be detected within a mixed population of cancer cells and non-cancer cells. The invention also relates to compositions and methods that may be used in cancer cell detection, specifically viruses that are replication-competent conditional to a cancer cell, in particular an oncolytic herpes virus, such as NV 1066 and a vaccinia virus, such as GLV-1h68. Provided are methods and kits for using these viruses that preferentially replicate in cancer cells and may also preferentially infect cancer cells for specific identification of such cancer cells, even when a cancer cell is present, for example, at a ratio of one infected cancer cell in a background often thousand non-cancer cells, thus further providing a reproducible and sensitive screening method for cancer detection, monitoring and prognosis. | 12-17-2009 |
20090311665 | Methods, compositions, and kits for collecting and detecting oligonucleotides - Methods, pharmaceutical compositions, and kits are provided which includes accurately sampling a RNA from a tissue of an animal and analyzing RNA in the tissue of the animal as an indicator of physiological state, infectious disease, neoplastic disease, autoimmune disease, inflammatory disease, cardiovascular disease, atherosclerotic disease, or neurological disease in the animal. A method is provided which includes administering at least one compound to an animal wherein the at least one compound is configured to prevent the cleavage of at least one tissue RNA by a ribonuclease. The method further includes collecting a sample of at least a portion of tissue from the animal. | 12-17-2009 |
20090311666 | MICROFLUIDIC DEVICE FOR CRYSTALLIZATION AND CRYSTALLOGRAPHIC ANALYSIS OF MOLECULES - The present invention relates to a microfluidic device comprising at least one crystallization chamber adapted for comprising a solution in which at least one compound is present according to a concentration gradient, and wherein the geometry of said crystallization chamber allows for convection phenomena to be limited. The invention also relates to the use of said device, in particular for crystallization by counter diffusion and to a crystallization method. | 12-17-2009 |
20090311667 | RAPID DIAGNOSIS METHOD SPECIFIC TO AVIAN INFLUENZA VIRUS - The present invention relates to a method for detecting an avian influenza virus by an immunological assay using an anti-influenza virus antibody being unreactive to human influenza type-A virus subtypes H1, H2 and H3 and a human influenza type-B virus and being reactive to plural subtypes of avian influenza viruses, and an immunochromatographic test tool for use in the method. According to the present invention, an avian influenza virus can be detected specifically, rapidly and in a simple manner, as distinguishing an avian influenza virus from a human influenza virus. | 12-17-2009 |
20090311668 | In situ detection of early stages and late stages HPV einfection - Embodiments of the invention provide methods, monoclonal antibodies, polyclonal antibodies, assays, and kits for detecting HPV infection and HPV related cancer diagnosis, including infection by various HPV genotypes, early and/or late stage HPV-associated or HPV-specific cancers. The anti-HPV antibodies are used in performing immunological assays on clinical samples. Various immunological assays and kits for detecting HPV infection, cervical cancer, other HPV related cancers, early stage precancerous lesions as well as late stage cancer progression are also provided. | 12-17-2009 |
20090311669 | SIGNAL FOR PACKAGING OF INFLUENZA VIRUS VECTORS - The invention provides a packaging (incorporation) signal for influenza virus vectors, and methods of using the signal to transmit and maintain influenza viral and foreign nucleic acid in virus and cells. | 12-17-2009 |
20090311670 | APPARATUS AND METHOD FOR MEASURING CONCENTRATION OF MOLECULES THROUGH A BARRIER - An apparatus and method for detecting the presence and measuring the concentrations of molecules through a barrier and/or at a distance utilizes the principle of chemical/electrostatic attraction (hereinafter “affinity”), i.e., the affinity between charged atoms and molecules that cause their chemical interactions and reactions, to infer, based on the behavior of molecules on one side of the barrier, the presence and concentration of corresponding molecules on the other side of the barrier. The invention is useful, by way of example and not limitation, in non-invasively measuring glucose levels of diabetic patients. | 12-17-2009 |
20090317794 | BIOLOGICAL INDICATOR - A kinase is used in a biological indicator for validation of treatment processes designed to reduce the amount or activity of a biological agent in a sample. The indication can be used for validation of sterilisation treatment. The formation of ATP from a substrate comprising ADP is measured via the liciferin/luciferate system in a luminameter. Thermostable adenylate kinase from | 12-24-2009 |
20090317795 | METHOD OF DETECTING H5 OR H7 AVIAN INFLUENZA VIRUS - The present invention provides oligonucleotide primers specifically hybridizing to an arbitrary nucleotide sequence designed from the nucleotide sequence of hemagglutinin of an H5 or H7 avian influenza virus, a nucleic acid amplification method using the primers, a method for diagnosis of infection with an H5 or H7 avian influenza virus by detection of nucleic acid amplification, and a kit for influenza diagnosis. | 12-24-2009 |
20090317796 | Indirect immunofluorescence assay typing kit for coxsackievirus A group and method for typing coxsackievirus A group - An indirect immunofluorescence assay typing kit for coxsackievirus, comprising: a first reagent comprising a mixture of an anti-coxsackievirus A2 polyclonal antibody, an anti-coxsackievirus A4 polyclonal antibody, an anti-coxsackievirus A5 polyclonal antibody, an anti-coxsackievirus A6 polyclonal antibody, and an anti-coxsackievirus A10 polyclonal antibody; a second reagent comprising the anti-coxsackievirus A2 polyclonal antibody; a third reagent comprising the anti-coxsackievirus A4 polyclonal antibody; a fourth reagent comprising the anti-coxsackievirus A5 polyclonal antibody; a fifth reagent comprising the anti-coxsackievirus A6 polyclonal antibody; a sixth reagent comprising the anti-coxsackievirus A10 polyclonal antibody; and a seventh reagent comprising a secondary antibody labeled with a fluorescence compound, wherein the secondary antibody is used for detecting the antibody anti-coxsackieviruses A2, A4, A5, A6 and A10 polyclonal antibodies and a titer of the anti-coxsackieviruses A2, A4, A5, A6 or A10 polyclonal antibody is about 1:5000-151:70000. | 12-24-2009 |
20090325145 | METHODOLOGY FOR ANALYSIS OF SEQUENCE VARIATIONS WITHIN THE HCV NS5B GENOMIC REGION - The current invention relates to a standardized method for amplification of an HCV NS5B nucleic acid fragment of any one of HCV genotypes 1 to 6 as a tool for analysis of sequence variations that may be correlated with HCV drug resistance. | 12-31-2009 |
20090325146 | Method of determining susceptibility of a tumor cell to a chemotherapeutic agent: novel use of herpes - The present invention provides a method of determining if a tumor cell is susceptible to killing by a chemotherapeutic agent, comprising: (a) providing a tumor cell; (b) infecting said tumor cell with a herpes simplex virus or a herpes simplex virus defective in an immediate early gene selected from the group consisting of ICP27, ICP4, and ICP22; and (c) determining the presence of apoptotic killing of said tumor cell, wherein the presence of apoptotic killing is indicative of susceptibility to said chemotherapeutic agent. Chemotherapeutic agent may include doxorubicin, etoposide, paclitaxel, cisplatin, or 5-fluorouracil. The present invention also provides a herpes simplex virus promoter construct having a lacZ gene to assess tumor resistance to chemotherapeutic agents. | 12-31-2009 |
20090325147 | MOLECULARLY IMPRINTED POLYMERS FOR DETECTING MICROORGANISMS - The invention described herein provides molecularly imprinted polymers (MIPs) that are capable of binding to a microorganism, and methods for detecting and/or identifying microorganisms utilizing Molecularly Imprinted Polymers (MIPs). The microorganisms of the invention include prokaryotes, eukaryotes, virus and prions. The methods of the invention comprise detecting all or part, including epitopes, of macromolecules associated with the microorganisms. The macromolecules of the invention include polysaccharides, proteins, glycoproteins, peptidoglycans, lipoproteins, peptides, polypeptides, and polynucleotides, associated with said microorganisms. The invention also provides for methods of diagnosing a subject infected with the microorganisms utilizing MIPs, in addition to diagnostic kits. | 12-31-2009 |
20090325148 | Bead Array Reader Based-Hemagglutination and Hemagglutination Inhibition Assay - Hemagglutination assays and hemagglutination inhibition assays were introduced in medical and virology practice more than 60 years ago. Since then, these assays have become important tools for measuring concentrations and strengths of viral cultures, the efficacy of the anti-viral immunization, and for studying the neutralizing capacity of virus-specific antibodies. The present invention comprises an improved hemagglutination inhibition assay (HAI), with at least about a 10-fold increase in sensitivity versus the traditional the HAI, to provide more accurate measurements of components in, for example, fluids from the in vitro MIMIC® system when assessing the effects of anti-viral vaccines (e.g., for seasonal influenza). | 12-31-2009 |
20090325149 | DETECTION AND DISTINGUISHING INFECTIONS BURSAL DISEASE VIRUS (IBDV) STRAINS BY MOLECULAR BIOLOGY METHOD - The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transcriptase-polymerase chain reaction. Two different primer combinations (Primer IF & IVIR) and (Primer IF & RCLA) and real-time polymerase chain reaction conditions were designed and optimized for rapid differentiation of very virulent and vaccine strains of IBDV based on detection of signatory threshold cycle (Ct) and melting temperature (Tm) values. | 12-31-2009 |
20100003665 | Real-time HPV PCR Assays - The present invention relates a fluorescent multiplex PCR assay for detecting the presence of an HPV type in a sample using multiple fluorophores to simultaneously detect a plurality of HPV genes of the same HPV type, wherein the HPV type is selected from the group consisting of: HPV33, HPV35, HPV39, HPV51, HPV56, and HPV59. The present invention also relates to oligonucleotide primers and probes specific to said HPV types for use in the methods of the present invention. | 01-07-2010 |
20100003666 | Microfluidic Methods for Diagnostics and Cellular Analysis - Methods for detection of molecular recognition and analysis of cells are provided. Both optical and non-optical methods are presented. Methods utilize capture of particles in semi-permeable structures. Specific microfluidic system architectures for conducting biomolecule and cell assays are described. | 01-07-2010 |
20100003667 | MULTIPLE LABELLING FOR ANALYTE DETECTION - Provided is use of a label in a method for detecting an analyte in a sample, for increasing the sensitivity of detection of the analyte, wherein, the analyte comprises a repeating protein unit, and wherein a plurality of label entities are capable of binding to a single analyte entity such that a signal obtained for an analyte from a plurality of labels is stronger than the signal obtained for an analyte with a single label. | 01-07-2010 |
20100003668 | Oligonucleotides and Use Thereof for Determining Deletion in HBV Pre-S Region - This invention provides combinations of novel oligonucleotides and their use in detecting a deletion(s) in the Pre-S region of HBV. Such a deletion(s) is associated with an increased risk of developing cirrhosis or hepatocellular carcinoma. | 01-07-2010 |
20100009338 | Novel gold nanostructures and methods of use - The invention is drawn to novel nanostructures comprising hollow nanospheres and nanotubes for use as chemical sensors, conduits for fluids, and electronic conductors. The nanostructures can be used in microfluidic devices, for transporting fluids between devices and structures in analytical devices, for conducting electrical currents between devices and structure in analytical devices, and for conducting electrical currents between biological molecules and electronic devices, such as bio-microchips. | 01-14-2010 |
20100009339 | Detection method for influenza viruses - A method of rapid detection of influenza viruses and/or virus particles comprising a hemagglutinin and a neuraminidase component, said method comprising the steps of: | 01-14-2010 |
20100009340 | COMPOSITIONS AND METHODS FOR THE ANALYSIS AND TREATMENT OF AIDS WASTING SYNDROME AND IMMUNE CELL DYSFUNCTION - The present invention relates to aberrant cell signaling by the HIV type I envelope glycoprotein. In particular, the present invention provides compositions and methods for identification of inhibitors of melanocortin receptor pathway stimulation by HIV-I gp120 and its degradation products. The inhibitors so identified are contemplated to be suitable for treating HIV-related cachexia, T cell hyperactivation and central nervous system disease. | 01-14-2010 |
20100009341 | METHODS AND MEANS FOR ASSESSING HIV GAG/PROTEASE INHIBITOR THERAPY - The present invention relates to methods and means for the evaluation of HIV treatment. In particular, molecular events at the HIV gag and protease proteins and their effect on therapeutic efficacy of drugs are determined The methods rely on providing HIV gag and protease nucleic acid material and evaluating a treatment either through genotyping or phenotyping. Said method may find use in multiple fields including diagnostics, drug screening, pharmacogenetics and drug development. | 01-14-2010 |
20100009342 | CONTROL OF CHEMICAL MODIFICATION - Provided is a method for controlling the degree of labeling (DOL) of a carrier molecule or solid support by the addition of a reactive label competitor to the labeling reaction. When the reactive label competitor is added to the labeling solution the competitor competes with the carrier molecule or solid support for the label, reducing the number of labels available to conjugates to the carrier molecule or solid support. This provides for a facile method that predictably alters the DOL of a carrier molecule or solid support. | 01-14-2010 |
20100009343 | COMPOSITIONS AND METHOD FOR RAPID, REAL-TIME DETECTION OF INFLUENZA A VIRUS (H1N1) SWINE 2009 - Disclosed are oligonucleotide amplification primers and detection probes specific for the amplification and detection of pathogenic organisms, including for example, specific Influenza A H1N1 viral isolates. Also disclosed is a biological organism identification kit including the disclosed nucleic acid probes and primers, as well as thermal cycling reagents that is both portable and durable, and may also be self-contained for remote, or in-field analysis and identification of particular influenza isolates from a variety of biological specimen types. | 01-14-2010 |
20100009344 | LIQUID CRYSTAL BASED ANALYTE DETECTION - The present invention relates to the field of detection of viruses, and in particular to detection of viruses using a liquid crystal assay format. In the present invention, virus binding in a detection region is identified by changes in liquid crystal orientation caused by virus binding independent orientation caused by any topography associated with the detection region. | 01-14-2010 |
20100015594 | HUMAN PAPILLOMA VIRUS (HPV) DETECTION USING NUCLEIC ACID PROBES, MICROBEADS AND FLUORESCENT-ACTIVATED CELL SORTER (FACS) - The present invention relates generally to the field of diagnostic and detection assays. More particularly, the present invention provides methods, and reagents including biochips for detecting the presence of, or distinguishing between, one or more analytes in a sample. | 01-21-2010 |
20100015595 | Phage Particle Diagnostic Reagents - The present invention relates to novel methods for detecting a member of a known binding pair in a sample, including a cell, where one member of the pair (termed the “receptor”) is expressed by a bacteriophage, which phage is then used to detect the presence of the other member of the pair (termed the “ligand” or “target”). Rather than detecting the binding of the phage using antibody-based technology, the present invention relates to detecting marker molecule associated with the phage. In one aspect, the invention relates to identifying an antigen-bearing moiety (e.g., a red blood cell antigen) of interest present on a cell, e.g., a red blood cell, using antibody-displaying bacteriophage, as well as detecting anti-red blood cell auto- or alloantibodies and/or complement in a sample, using antiglobulin reagent-displaying bacteriophage and detecting a marker molecule associated with the phage. In one aspect, the phenotype of the phage is not linked with the genotype of the phage. | 01-21-2010 |
20100015596 | RS VIRUS DETECTING KIT USING ANTI-RS VIRUS MONOCLONAL ANTIBODY, IMMUNO-CHROMATOGRAPHIC TEST DEVICE, AND NEW ANTI-RS VIRUS MONOCLONAL ANTIBODY - A kit or an immuno-chromatographic test device for detection of respiratory syncytial virus (RSV), comprising at least an RSV F protein-recognizing anti-RSV monoclonal antibody produced by hybridoma RSF2-412. An anti-RSV monoclonal antibody recognizing an RS virus F protein, which is selected from the group consisting of an antibody produced by hybridoma RSF2-412, an antibody produced by hybridoma RSF1-1565, and an antibody produced by hybridoma RSF6-255. | 01-21-2010 |
20100015597 | PRODUCTION OF BIOLOGICAL CARRIERS FOR INDUCTION OF IMMUNE RESPONSE AND INHIBITION OF VIRAL REPLICATION - This application provides a method to form non-infectious Biological Carrier that may be used to deliver signals to cells either in vitro or in vivo. The Biological Carriers are inactivated virus particles that have been specifically modified to give biological properties different from the virus particles deriving from an unmodified host cell that (i) expresses at least one co-stimulatory molecule and (iia) at least one antigen that can initiate an immune response, and/or (iib) express surface molecules that suppress viral replication. | 01-21-2010 |
20100015598 | Light Emission Modifiers and Their Uses in Nucleic Acid Detection, Amplification and Analysis - The present invention relates to methods and reagents for modifying the emission of light from labeled nucleic acids for the purpose of real time detection, analysis, and quantitation of nucleic acid sequences, e.g., using singly labeled probes. These methods and reagents exploit advantageous properties of thiazine dyes and diazine dyes. Furthermore, the use of these light emission modifiers in background reduction, nucleic acid duplex stabilization and other uses is also described. Related kits, reaction mixtures and integrated systems are described. | 01-21-2010 |
20100021882 | METHOD TO DETECT VIRUS RELATED IMMUNOLOGICAL MARKERS FOR THE DIAGNOSIS OF RESPIRATORY TRACT INFECTIONS - This invention discloses using SPR technology to simultaneously and qualitatively detect the presence of respiratory tract viruses-related immunological markers in a serum sample, which can be used for the diagnosis of respiratory tract infections. It also discloses an efficient formula to make a mixed SAM that can greatly enhance the immobilization ability of the metal surface in SPR based techniques, which is good for the immobilization of representative antigens used to detect the respective respiratory tract viruses-related immunological markers (antibodies) in blood for the diagnosis of respiratory tract infections. | 01-28-2010 |
20100028853 | OPTICAL DETERMINATION OF LIVING VS. NON LIVING CELLS - A method of determining whether a cell sample in a medium contains living or dead cells of a predetermined target cell type is disclosed. The method includes preparing a testing substrate by attaching a binding molecule thereto, the binding molecule having the property of immobilizing cells of the target cell type upon coming in contact therewith. The method also includes incorporating a colorimetric indicator onto the testing substrate, performing a first spectrographic analysis of the calorimetric indicator, determining a change in pH of the medium based upon a second spectrographic analysis of the colorimetric indicator as compared to the first, and determining the portion of live target cells in the medium based upon the change in pH. | 02-04-2010 |
20100028854 | METHODS FOR MODELING INFECTIOUS DISEASE AND CHEMOSENSITIVITY IN CULTURED CELLS AND TISSUES - The present invention provides methods for utilizing a form of optimized suspension culture to examine the infectivity of pathogenic organisms and agents in human cells and tissues. Also provided are methods using a rotating wall vessel to predict Chemosensitivity of cells and tissues to toxins and chemotherapeutic agents. These culture conditions potentiate spatial colocalization and three-dimensional assembly of individual cells into large aggregates which more closely resemble the in vivo tissue equivalent. In this environment, dissociated cells can assemble and differentiate into macroscopic tissue aggregates several millimeters in size. These culture conditions allow for better cellular differentiation and formation of three-dimensional cellular aggregates, more efficient cell-to-cell interactions, the in in vivo-like exchange of growth factors and greater molecular scaffolding facilitating mechanical stability for cells. The suspension culture system offers a new approach for studying microbial infectivity from the perspective of the host-pathogen interaction and also for analyzing chemosensitivity to toxins and chemotherapeutic agents. | 02-04-2010 |
20100028855 | DETECTION OF INFLUENZA VIRUS TYPE B - The invention provides methods for detecting influenza B from its NS1 protein. The NS1 protein is present in detectable levels in clinical samples and can be detected using antibodies that are panspecific for influenza B without binding to influenza A or other viruses. | 02-04-2010 |
20100028856 | METHOD TO DETECT VIRUS RELATED IMMUNOLOGICAL MARKERS FOR THE DIAGNOSIS OF HEPATITIS B VIRUS INFECTION - This invention discloses using SPR technology to simultaneously and qualitatively measure the presence of HBV-associated immunological markers in a serum sample for the diagnosis of HBV infection. It also discloses an efficient formula to make a mixed SAM that can greatly enhance the immobilization ability of the metal surface in SPR based techniques, which is good for the immobilization of HBV related antigens or antibodies used for the diagnosis of HBV infection. | 02-04-2010 |
20100028857 | Method for Detecting and for Removing Endotoxin - The invention relates to a method for identifying endotoxins for eliminating said endotoxins from a sample, with the aid of bacteriophage tail proteins. | 02-04-2010 |
20100028858 | HIGH SENSITIVITY ASSAY FOR MOLECULAR TYPING OF BIOLOGICAL SAMPLE, PROBES AND A KIT THEREOF - The present invention relates to a high sensitivity assay for molecular typing of a biological sample using surface-enhanced Raman scattering (SERS) including resonance scattering (SERRS); capture probes for capturing nucleic acid; a detector probe to detect captured nucleic acid; a kit for molecular typing of biological sample using surface-enhanced Raman scattering (SERS) including resonance scattering (SERRS); and lastly a method of manufacturing said kit. | 02-04-2010 |
20100028859 | Hyper-Spectral Imaging and Analysis of a Sample of Matter, and Preparing a Test Solution or Suspension Therefrom - Method for hyper-spectral imaging and analysis of a sample of matter, for identifying and characterizing an object of interest therein. Preparing test solution or suspension of the sample, including adding thereto a spectral marker specific to object of interest, such that if object of interest is in test solution or suspension, object of interest becomes a hyper-spectrally active target which is hyper spectrally detectable and identifiable; adding to test solution or suspension a background reducing chemical, for reducing background interfering effects caused by presence of objects of non-interest in test solution or suspension, thereby increasing hyper spectral detectability of hyper spectrally active target in test solution or suspension; generating and collecting hyper-spectral image data and information of test solution or suspension; and, processing and analyzing thereof. Exemplary objects of interest are biological agents—bacteria ( | 02-04-2010 |
20100028860 | Porcine Reproductive and Respiratory Syndrome Isolates and Methods of Use - A method of predicting the virulence of a new or uncharacterized PRRS virus strain is provided wherein the strain is injected into swine and allowed to replicate for a period of from about 3-15 days. During this period, the rate of virus growth and/or the magnitude of viremia is determined, and this data is compared with a corresponding growth rate and/or viremia magnitude of a PRRS virus strain of known virulence, as a measure of the virulence of the new or uncharacterized strain. | 02-04-2010 |
20100035227 | Compositions for use in identification of alphaviruses - The present invention provides oligonucleotide primers and compositions and kits containing the same for rapid identification of alphaviruses by amplification of a segment of viral nucleic acid followed by molecular mass analysis. | 02-11-2010 |
20100035228 | Monitored separation device - A device for separating and purifying useful quantities of particles comprises:
| 02-11-2010 |
20100035229 | METHODS, PLASMID VECTORS AND PRIMERS FOR ASSESSING HIV VIRAL FITNESS - The present invention relates to methods and means for the evaluation of HIV replicative capacity in a given environment. In particular, the invention provides a growth competition assay that can determine relative viral fitness using a recombinant tagged HIV-1 virus system. The methods rely on plasmid vectors, amplicons, primers and probes, and the generation of replication-competent viruses therefrom. Said methods and materials may find use in multiple fields including diagnostics, drug screening, pharmacogenetics and drug development. | 02-11-2010 |
20100035230 | METHODS AND AGENTS FOR DETECTING PARECHOVIRUS - The present disclosure provides methods that permit detection of all known species of | 02-11-2010 |
20100035231 | ANTIGEN CAPTURE ANTI-DENGUE IGA ELISA (ACA-ELISA) FOR THE DETECTION OF A FLAVIVIRUS SPECIFIC ANTIBODY - An antigen capture IgA Enzyme Linked Immunosorbent Assay (ACA-ELISA) was developed for the detection of anti-flavivirus IgA. The assay utilizes flavivirus lysate antigen, preferably dengue virus lysate antigen captured by a monoclonal antibody. Captured anti-flavivirus IgA from test sera are preferably detected using rabbit anti-IgA conjugated with a reporter group such as horseradish peroxidase (HRP). The assay was found to be at least 8 times more sensitive than anti-human IgA capture ELISA (AAC-ELISA). The ACA-ELISA, based either on serum or saliva, was found to be more sensitive and rapid compared to the “gold standard” anti-dengue IgM detection technique and can be utilized as a diagnostic tool for the confirmation of dengue in the early phase of infection. | 02-11-2010 |
20100035232 | TARGETED WHOLE GENOME AMPLIFICATION METHOD FOR IDENTIFICATION OF PATHOGENS - The methods disclosed herein relate to methods and compositions for amplifying nucleic acid sequences, more specifically, from nucleic acid sequences of pathogens by targeted whole genome amplification. | 02-11-2010 |
20100035233 | Triggered RNAi - The present application relates to methods and compositions for triggering RNAi. Triggered RNAi is highly versatile because the silencing targets are independent of the detection targets. In some embodiments, methods of silencing or modulating the expression of a marker gene are provided. The methods generally comprise providing an initiator to a cell comprising a detection target and a silencing target gene, wherein the detection target is different from the silencing target gene, wherein binding of the detection target to the initiator initiates formation of an inactivator double-stranded RNA (inactivator dsRNA). The inactivator dsRNA can silence the silencing target gene to modulate the expression of a marker gene. | 02-11-2010 |
20100035234 | VACCINE ASSAYS - The present invention is directed to methods, assays and compositions for implementing such methods and assays for assessing efficacy of individual components in multi-component vaccines and for assessing efficacy of a vaccine against a pathogen. In one aspect, the method of assessing efficacy of a vaccine against a pathogen is a quick assay that tests for an activity correlated with efficacy such as binding in an ELISA rather than requiring the time and expense of an assay that detects actual bactericidal activity. In another aspect, the method for testing the efficacy of an individual component in a multi-component vaccine includes obtaining an immune sample from a subject inoculated with the multi-component vaccine; blocking the portion of the immune sample that recognizes the individual component such as by addition of the individual component, and testing the efficacy of the immune sample to respond to the pathogen. | 02-11-2010 |
20100035235 | USE OF FOCUSED LIGHT SCATTERING TECHNIQUES IN BIOLOGICAL APPLICATIONS - Methods for using focused light scattering techniques for the optical sensing of biological particles suspended in a liquid medium are disclosed. The optical sensing enables one to characterize particles size and/or distribution in a given sample. This, in turn, allows one to identify the biological particles, determine their relative particle density, detect particle shedding, and identify particle aggregation. The methods are also useful in screening and optimizing drug candidates, evaluating the efficacy and dosage levels of such drugs, and in personalized medicine applications. | 02-11-2010 |
20100035236 | ENRICHMENT METHOD FOR VARIANT PROTEINS WITH ALTERED BINDING PROPERTIES - A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants. | 02-11-2010 |
20100035237 | NOVEL ANTIGEN CONSTRUCTS USEFUL IN THE DETECTION AND DIFFERENTIATION OF ANTIBODIES TO HIV - Isolated HIV-1 Group O env polypeptides obtained from the HIV-1 isolate HAM112 are claimed, as well as (a) antigen constructs comprising fusions of one or more of each of HIV-1 Group O env polypeptides and HIV-1 Group M env polypeptide and (b) further antigen constructs containing additional Group O sequences and especially the gp41 IDR of isolate HAM112. Also claimed are polynucleotide sequences encoding the above, expression vectors comprising the same, host cells transformed thereby, and immunoassay methods and kits utilizing the antigen constructs of the invention. | 02-11-2010 |
20100041013 | ASSAY FOR A HEALTH STATE - The invention relates to an assay for determining a health state of a subject using a combination of detecting the presence of a virus and detecting the presence of a genomic target or marker indicative of a health state. | 02-18-2010 |
20100041014 | Biological targeting compositions and methods of using the same - Modified red blood cells are described. In an embodiment, the modified red blood cell includes a target-binding agent. Targeted delivery of imaging agents, drugs, and peptide and protein pharmaceuticals using modified red blood cells are described. Processes for preparing the modified red blood cells, pharmaceutical and diagnostic compositions containing the same and methods of diagnosis and treatment involving the modified red blood cells are described. | 02-18-2010 |
20100041015 | COMPETITIVE ENZYME LINKED IMMUNOSORBENT ASSAY (C-ELISA) FOR THE DETECTION OF A FLAVIVIRUS SPECIFIC ANTIBODY - A competitive enzyme-linked immunosorbent assay (C-ELISA), using flavivirus member specific immunological agents was developed to detect antibody specific to members of the flaviviruses indicative of exposure to flavivirus. The test is based on a competition for epitope binding on the envelope protein of the flavivirus antigen captured using anti-flavivirus IgA in the presence of flavivirus positive serum. This test has comparable sensitivity specificity and speed to the virus neutralization assay (VNT). C-ELISA is a versatile technique, which could have various applications. Slight modifications of this protocol could lead to a C-ELISA-based detection method of secondary infection or one that could be used for serotype specific sero-epidemiological studies and/or vaccine evaluation. The protocol developed for C-ELISA was demonstrated using dengue lysate antigen and dengue specific monoclonal antibody. This can be used against other flaviviruses and the results for Japanese encephalitis illustrates this. | 02-18-2010 |
20100041016 | METHODS FOR DETECTING ORGANISMS AND ENZYMATIC REACTIONS USING RAMAN SPECTROSCOPY AND AROMATIC COMPOUNDS COMPRISING PHOSPHATE - The present disclosure provides systems for the rapid and sensitive detection of organisms and molecules in samples. Reactants that produce Raman-active products are used in combination with Raman light scattering. Such compounds may comprise phosphates permitting the detection of phosphatases. The present disclosure can also be used to measure enzyme-kinetics. | 02-18-2010 |
20100041017 | FLUORESCENT SEMICONDUCTOR MICROPARTICLE ASSEMBLY, FLUORESCENT LABELING AGENT ASSEMBLY FOR BIOLOGICAL SUBSTANCE, AND BIOIMAGING METHOD AND BIOLOGICAL SUBSTANCE ANALYSIS METHOD USING THE ASSEMBLIES - Disclosed is a fluorescent semiconductor microparticle assembly comprising at least three kinds of fluorescent semiconductor microparticles with an average particle size of from 1 to 10 nm, having the same chemical composition, a different average particle size and a different emission maximum wavelength in the emission spectra, wherein a standard deviation of emission intensity in each of the at least three kinds of fluorescent semiconductor microparticles is not more than 15%. | 02-18-2010 |
20100041018 | METHOD TO DETECT VIRUS RELATED IMMUNOLOGICAL MARKERS FOR THE DIAGNOSIS OF HEPATITIS C VIRUS INFECTION - This invention discloses using SPR technology to simultaneously and qualitatively measure the presence of HCV-associated immunological markers in a serum sample for the diagnosis of HCV infection. It also discloses an efficient formula to make a mixed SAM that can greatly enhance the immobilization ability of the metal surface in SPR based techniques, which is good for the immobilization of HCV related antigen proteins used for the diagnosis of HCV infection. | 02-18-2010 |
20100041019 | Methods of Screening for Respiratory Synctial Virus and Human Metapneumovirus - Provided are nucleic acid primers and probes for use in diagnostic assays to screen for respiratory infections, such as respiratory syncytial virus (“RSV”) and human metapneumovirus (hMPV). The primers and probes may be used to screen for RSV or hMPV in a singleplex assay or they may be used in a multiplex assay to simultaneously screen for RSV and hMPV, or RSV and/or hMPV and any of the following viruses: influenza A, and influenza B, parainfluenza viruses, adenovirus, coronavirus, and rhinoviruses. | 02-18-2010 |
20100041020 | Methods for detecting the presence, location or quantity of targets using novel dyes - The present invention provides dyes, reactive dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins and nucleic acids. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes are also provided that comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been modified by the addition of charged and polar groups to provide beneficial properties. | 02-18-2010 |
20100041021 | APPARATUS AND METHOD FOR MEASURING CONCENTRATION OF MOLECULES THROUGH A BARRIER - An apparatus and method for detecting the presence and measuring the concentrations of molecules through a barrier and/or at a distance utilizes the principle of chemical/electrostatic attraction (hereinafter “affinity”), i.e., the affinity between charged atoms and molecules that cause their chemical interactions and reactions, to infer, based on the behavior of molecules on one side of the barrier, the presence and concentration of corresponding molecules on the other side of the barrier. The invention is useful, by way of example and not limitation, in non-invasively measuring glucose levels of diabetic patients. | 02-18-2010 |
20100041022 | Novel assay for the separation and quantification of hemagglutinin antigens - Described are methods for separating hemagglutinin (HA) antigens, comprising the steps of applying a reduced and derivatized antigen preparation comprising solubilized HA antigens and a detergent in a pH controlled solution, on a Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) column; and eluting the HA antigens from the column with an ion pairing agent in an organic mobile phase. The invention further relates to quantifying methods using the methods for separating the antigens with the further step of measuring the peak area of the eluted antigen in a chromatogram resulting from the elution step. | 02-18-2010 |
20100047763 | Plasmid Expression Vectors for Expression of Recombinant Rotavirus and Astrovirus Proteins or Epitopes - The present invention refers to the production of specific recombinant viral proteins intended for use in the construction of a diagnostic kit for the simultaneous detection of the two most important gastroenteric viruses, namely rotavirus and astrovirus. | 02-25-2010 |
20100047764 | Novel surface protein (HBsAg) variant of the hepatitis B virus - The invention relates to sequences of a novel variant of the Hepatitis B surface antigen (HBsAg) and to methods for detecting, in patient samples, nucleic acids, antigens, and antibodies directed against the same. | 02-25-2010 |
20100047765 | MOLECULAR DETERMINANTS ASSOCIATED WITH ENHANCED ABILITY TO ENTER CELLS EXPRESSING CXCR4 - The invention provides a method for determining whether a human immunodeficiency virus is likely to be have enhanced ability to enter a cell expressing CD4 and CXCR4 relative to a reference HIV. In certain aspects, the methods comprise detecting one or more amino acid in an envelope protein of the HIV associated with enhanced ability to enter CD4- and CXCR4-expressing cells and determining that the HIVs ability to enter such cells is enhanced relative to a reference HIV, e.g., an HIV that does not comprise such amino acid(s). | 02-25-2010 |
20100047766 | ANALYTE MANIPULATION AND DETECTION - Provided is a method for separating two or more analytes in a fluid, which method comprises:
| 02-25-2010 |
20100047767 | PATHOGEN BINDING - Provided is a method for determining the presence or absence of a pathogen in a sample, which method comprises: a) contacting the sample with a whole or a part of a cell surface receptor protein capable of binding the pathogen; b) allowing the cell surface receptor protein or part thereof to bind the pathogen; c) determining the presence or absence of the pathogen bound to the receptor protein or part thereof. | 02-25-2010 |
20100047768 | AMPLIFICATION OF SINGLE VIRAL GENOMES - The present invention relates, e.g., to a method for amplifying the genome of a single virus particle from a mixture of virus particles, comprising (a) subjecting the mixture of virus particles to flow cytometry and identifying a sorted sample that putatively contains a single virus particle, (b) imbedding the sorted sample comprising the putative single viral particle in a solid matrix (e.g., low melting agarose); (c) visualizing the embedded virus particle (e.g., by EFM and/or confocal microscopy) to confirm that a single particle is embedded; and (d) exposing the nucleic acid from the visualized, embedded single, discrete viral particle (e.g., by alkali treatment) and amplifying the genomic viral nucleic acid in situ (e.g., by MDA). | 02-25-2010 |
20100055669 | Generation of Recombinant Genes in Bacteriophages - In vivo methods for generating and detecting recombinant DNA sequences in bacteriophages or plasmids containing bacteriophage sequences, methods for generating hybrid genes and hybrid proteins encoded by these hybrid genes by the use of bacteriophages and plasmids containing bacteriophage sequences, bacteriophages and plasmids that can be used in these methods, and kits comprising appropriate bacterial host cells and bacteriophages or plasmids are described. | 03-04-2010 |
20100055670 | GROWTH OF WILD-TYPE HEPATITIS A VIRUS IN CELL CULTURE - The invention provides recombinant Hepatitis A Virus (HAV) nucleic acids and host cells that are permissive for their growth and replication. The recombinant Hepatitis A Virus nucleic acids not particularly limited, except that they incorporate at least one heterologous nucleic acid fragment. The heterologous nucleic acid can encode a selectable marker gene and such recombinant HAV nucleic acids are useful for selecting cells that are permissive for growth and replication of wild type HAV. Alternatively, the heterologous nucleic acid may encode a vaccine antigen or other expression product that is desirable to express in a cell harboring the recombinant HAV nucleic acid. The invention further provides cell lines permissive for growth and replication of wild type HAV or HAV having minimal mutations for growth in cell culture. The invention further provides methods for producing HAV vaccines and for monitoring environmental and patient samples for the presence of HAV. | 03-04-2010 |
20100055671 | Method for Determining Transduction Efficiency and Virus Dosage of Baculovirus - A method for determining transduction efficiency of baculovirus includes: providing a recombinant baculovirus, in which the recombinant baculovirus includes an inducible promoter and a reporter gene positioned downstream the inducible promoter; adding the recombinant baculovirus to incubating environment of a mammalian cell for transduction; adding an inducer to promote expression of the reporter gene in the mammalian cell; and analyzing the percentage of the mammalian cell expressing the reporter gene to determine the transduction efficiency of the recombinant baculovirus. The method provides the ability to quantitatively analyze baculovirus transduction and is a simple and faster quantitative method applicable in transduction and other research study by including an inducible promoter and a reporter gene and thus prevents from imposing excessive metabolic burden to the cells. A method for determining virus dosage of baculovirus applied in genetic therapy is also disclosed. | 03-04-2010 |
20100055672 | 100% SEQUENCE IDENTITY DETECTION METHODS FOR VARIABLE GENOMES - The present disclosure provides methods, reagents and kits for the detection of all known human variants of influenza A virus and at least 90% of avian and swine variants of influenza A virus in a biological sample, based on amplification primers and detection probes that are specific to a highly conserved region of the influenza A matrix gene. | 03-04-2010 |
20100055673 | TRANSPARENT MICROFLUIDIC DEVICE - A device for analysing the status of a biological entity. The device ( | 03-04-2010 |
20100055674 | UTILIZING LIVER CELL LINE QSG-7701 TO BE INFECTED WITH HEPATITIS B VIRUS - The use of the liver cell line QSG-7701 for HBV infection includes the following steps: directly infecting QSG-7701 cells with purified HBV particles and facilitating the infection by DMSO and/or PEG treatment. The easily available QSG-7701 liver cell line may not require pre-differentiation induction and is naturally susceptible for HBV infection. This cell line provides near normal physiological conditions for HBV infection, especially the infection conditions that are characterized with Chinese origin. This cell line is suitable for investigating the life cycle of HBV. Therefore, this cell line is useful for the investigation of viral infection processes and for the development of drugs that specifically target these processes. | 03-04-2010 |
20100055675 | METHOD FOR DETECTING MEASLES VIRUS, MEMBRANE ASSAY TEST DEVICE, AND MEMBRANE ASSAY TEST KIT - Method for detecting a measles virus in an analyte, comprising forming a complex of a first monoclonal antibody being capable of binding to a first epitope of a measles virus nuclear protein and being immobilized on a solid phase, a second monoclonal antibody being capable of binding to a second epitope of a measles virus nuclear protein different from the first epitope and being labeled, and a measles virus nuclear protein contained in the analyte, on the solid phase; and detecting the measles virus based on the amount of the label of the complex formed on the solid phase, is disclosed. Membrane assay test device, and membrane assay test kit are also disclosed. | 03-04-2010 |
20100062415 | Pathogen Detection Biosensor - The invention described herein provides methods for the detection of target particles, such as pathogens, soluble antigens, nucleic acids, toxins, chemicals, plant pathogens, blood borne pathogens, bacteria, viruses and the like. Also described is an emittor cell comprising a receptor, wherein the receptor can be an antibody or an Fc receptor, and an emittor molecule for the detection of a target particle in a sample wherein the target particle to be detected is bound by one or more receptors on the emittor cell. Also provided are optoelectronic sensor devices for detecting a target particle in a sample, including in a plurality of samples. | 03-11-2010 |
20100062416 | Immobilisation and application of antigenic carbohydrates to detect infective micro-organisms - The invention relates to the field of chemistry and diagnosis, more in particular to diagnosis of current and/or past and/or symptomless infections or of a history of exposure to a gram-negative-bacterium (such as an enterobacteriaceae or a | 03-11-2010 |
20100062417 | ZEOLITE CRYSTALS WITH BIOLOGICAL MATERIAL - The invention provides a hybrid construct comprising at least a zeolite crystal and at least one biological moiety such as for example a cells bacteria or virus and a method for the production thereof. | 03-11-2010 |
20100062418 | INACTIVATED AND DRIED BIOLOGICAL PREPARATIONS - In one aspect, the present invention provides biological sample that includes a dry preparation comprising a stabilizer and stabilized, inactivated biological material. In certain embodiments, the biological material may be heat inactivated and/or heat dried. In another aspect, the present invention provides a method of making a dried biological preparation. Generally, the method includes collecting a sample of biological material, inactivating the biological material, suspending the sample in a volume of a stabilizer and allowing the stabilizer to dry. In certain embodiments, the biological material may be heat inactivated and/or heat dried. | 03-11-2010 |
20100062419 | Infectious, Chimeric Hepatitis C Virus, Methods of Producing the Same and Methods of Use Thereof - The present invention provides infectious recombinant Hepatitis C Viruses (HCV), and vectors, cells and animals comprising the same. The present invention provides methods of producing infectious recombinant HCV, and their use in identifying anti-HCV therapeutic agents, as well as sequences of HCV associated with HCV pathogenesis. | 03-11-2010 |
20100062420 | Type I interferon-inducible proteins to detect viral infection - A method for determining the presence of a viral infection in an animal not known to have been infected with a virus or other disease-causing microbial organism by determining the level of Mx protein or other Type I Interferon-inducible protein in the animal. | 03-11-2010 |
20100062421 | COMPOSITIONS, METHODS, AND DEVICES FOR ISOLATING BIOLOGICAL MATERIALS - Compositions, methods, devices, and kits, which include an immobilized-metal support material comprising a substrate having a plurality of —C(O)O | 03-11-2010 |
20100068696 | UNIVERSAL BIOSENSOR AND METHODS OF USE - The present invention relates to methods for detecting or quantifying an analyte in a test sample including providing at least one test mixture including a test sample, at least one marker complex, wherein each marker complex includes a particle, a marker, and one member of a coupling group, a first binding material selected to bind to a portion of the analyte, a second binding material selected to bind with a portion of the analyte other than the portion of the analyte for which the first binding material is selected, analyte analog, and/or marker conjugate. The at least one test mixture is passed through a membrane. The amount of marker on the membrane is detected and correlated to the presence or amount of analyte in the test sample. | 03-18-2010 |
20100068697 | PIEZOELECTRIC MICROCANTILEVER SENSORS FOR BIOSENSING - A piezoelectric microcantilever for sensing compounds or molecules. The piezoelectric microcantilever, may include at least one electrode, an insulation layer, a receptor, an immobilization layer, a non-piezoelectric layer and a piezoelectric layer The sensor is capable of self actuation and detection. The piezoelectric layer may be constructed from a highly piezoelectric thin lead magnesium niobate-lead titanate film, a highly piezoelectric thin zirconate titanate film, a highly piezoelectric lead-free film. Methods of using the sensors and flow cells and arrays including the sensors are also described. | 03-18-2010 |
20100068698 | PRODUCTION OF INFECTIOUS HEPATITIS C VIRUS PARTICLES IN CELL CULTURE - The present invention provides for novel methods of producing high levels of infectious HCV genotype 1 virus particles in cell culture systems. The availability of HCV genotype 1 virus (principally associated with liver disease in most regions of the world) that can undergo the complete viral cycle in cultured cells is beneficial for the discovery and development of novel therapies for the treatment of HCV. | 03-18-2010 |
20100075298 | Method for rapid identification and quantification of microorganisms - Method for unamplified, selective identification of a microorganism comprising obtaining a sample comprising the microorganism, wherein the sample comprises unseparated genetic material of the microorganism; adding to the sample a molecular beacon comprising an nucleic sequence which is complementary to at least one nucleic acid sequence in the microorganism; and heating the sample; wherein the molecular beacon hybridizes with said nucleic acid sequence to produce a detectable signal. | 03-25-2010 |
20100075299 | DETECTION AND USE OF ANTIVIRAL RESISTANCE MUTATIONS - The present invention relates generally to viral variants exhibiting reduced sensitivity to particular agents and/or reduced interactivity with immunological reagents. More particularly, the present invention is directed to hepatitis B virus (HBV) variants exhibiting complete or partial resistance to nucleoside or nucleotide analogs and/or reduced interactivity with antibodies to viral surface components including reduced sensitivity to these antibodies. Vaccines and diagnostic assays are also contemplated herein. | 03-25-2010 |
20100075300 | ARRAYED DETECTOR SYSTEM FOR MEASUREMENT OF ANTI-VIRAL IMMUNE RESPONSE - A sensor chip for detecting an immune response against a virus, the sensor chip including a substrate having a surface and a plurality of virus-like particles or capsid fragments bound to discrete locations on the surface of the substrate. Detection devices containing the sensor chip and methods of detecting anti-viral immune responses are also described herein. | 03-25-2010 |
20100075301 | Methods for Generation of Reporter Phages and Immobilization of Active Bacteriophages on a Polymer Surface - Novel reporter bacteriophages are provided. Provided are compositions and methods that allow bacteriophages that are used for specific detection or killing of | 03-25-2010 |
20100081123 | Devices and methods for analyte detection using distorted liquid crystals - The present invention provides devices and methods for detection of analytes based on measuring the anchoring strength of liquid crystals having distorted geometries. Methods for detecting an analyte in a sample include the steps of: (a) capturing an analyte on a substrate surface wherein the substrate surface defines an easy axis when in contact with a liquid crystal. Substrate surface and liquid crystal are brought into contact and an analyte-dependent departure in the orientation of the liquid crystal from the easy axis of the substrate surface is measured. This departure indicates the presence of the analyte in the sample. | 04-01-2010 |
20100081124 | PRIMERS AND PROBES FOR DETECTING HUMAN PAPILLOMAVIRUS AND HUMAN BETA GLOBIN SEQUENCES IN TEST SAMPLES - The present invention relates to primers, probes, primer sets, primer and probe sets, methods and kits for detecting human papillomaviruses, human beta globin sequences and human papillomaviruses and human beta globin sequences in a test sample. | 04-01-2010 |
20100081125 | AMPLIFICATION FOR SOLID PHASE IMMUNOASSAY - The present invention is directed to immunoassays for detecting one or more target analytes in a fluid sample wherein the detection reaction occurs on a solid support and involves an amplification system. In particular, the invention is directed to making and using a test device having at least one site for detecting the presence of at least one target analyte, wherein a conjugate comprising dextran-polystreptavidin is immobilized at the test site(s) as a capture reagent for a complex containing the target analyze. | 04-01-2010 |
20100081126 | PROCESS FOR DESIGNING INHIBITORS OF INFLUENZA VIRUS STRUCTURAL PROTEIN-1 - Disclosed are methods and compositions useful in identifying inhibitors of influenza virus, such as influenza A and B virus. Also disclosed are methods for preparing compositions for administration to animals, including humans infected with or to protect against influenza virus. | 04-01-2010 |
20100081127 | System and Method of Chemical Imaging Using Pulsed Laser Excitation and Time-Gated Detection to Determine Tissue Margins During Surgery - System and method for differentiating tissue margins in a biological sample using pulsed laser excitation and time-gated detection. A region containing a biological tissue is irradiated with substantially monochromatic pulsed laser light to thereby produce Raman scattered photons. The Raman scattered photons are detected using time-gated detection to thereby obtain a Raman spectroscopic image from the irradiated region characteristic of either a neoplastic portion or a non-neoplastic portion of the region containing the biological tissue. A boundary between a neoplastic portion and a non-neoplastic portion is differentiated and the boundary location in the Raman spectroscopic image is displayed. | 04-01-2010 |
20100086908 | METHODS FOR THE DETECTION OF RESPIRATORY VIRUSES - The present invention relates generally to the field of nucleic acid detection and more specifically to the detection of human respiratory viruses in a patient sample. In some aspects, the invention relates to the detection of multiple respiratory viral groups, including rhinovirus, respiratory syncytial virus, parainfluenza virus, influenza virus, metapneumovirus, adenovirus, coronavirus, and enterovirus. | 04-08-2010 |
20100092940 | Method and Device for Detecting Feline Immunodeficiency Virus - A method and device for determining a feline immunodeficiency virus infection or vaccination in an animal. The method includes contacting a biological sample from a felid with various FIV polypeptides and determining the binding of antibodies in the sample to the polypeptides. The determination of whether an animal is infected with FIV or has been vaccinated against FIV can be determined by measuring the animal's immune response to an FIV env polypeptide. A device for detecting FIV antibodies is provided. | 04-15-2010 |
20100092941 | 32142,21481,25964,21686, Novel dehydrogenase molecules and uses therefor - The invention provides isolated nucleic acids molecules, designated DHDR nucleic acid molecules, which encode novel DHDR-related dehydrogenase molecules. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing DHDR nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a DHDR gene has been introduced or disrupted. The invention still further provides isolated DHDR proteins, fusion proteins, antigenic peptides and anti-DHDR antibodies. Diagnostic methods utilizing compositions of the invention are also provided. | 04-15-2010 |
20100092942 | Method and apparatus for detection of biological organisms using Raman scattering - A system for the detection of compounds, including a target biological organism or component from a sample, using one or more reactant that will bind to the biological organism or compound forming a Raman active product, concentrating the Raman active product, and detecting the Raman active product using Raman light scattering. | 04-15-2010 |
20100092943 | SCREENING METHOD WITH THE USE OF TBK1 KNOCKOUT MOUSE - The present invention provides a method for screening inductive promoting substances of anti-viral proteins such as IFN-β against LPS stimulation or viral infection by using TBK1 knockout mice, or the tissues or cells derived therefrom. The present invention also provides a method for screening substances promoting responses against LPS stimulation or viral infection which may comprise the steps of measuring/estimating the induction level of anti-viral proteins such as IFN-β against ligands recognized by TLR4 or substances containing thereof in mice wherein a part or a whole of TANK binding kinase-1 (TBK1) genes on its chromosome is deleted and is lacking the function to express TBK1 which is expressed in wild-type, or the tissues or cells derived therefrom; by using the mice, or the tissues or cells derived therefrom, a test substance, and the ligands recognized by TLR4 or substances containing thereof. | 04-15-2010 |
20100092944 | DETECTION OF INFLUENZA VIRUS - The present application describes methods for detecting influenza A and/or influenza B and/or distinguishing between pathogenic and seasonal influenza A subtypes. Many of these preferred formats employ pan-specific antibodies (i.e., that react with all or at least multiple strains within an influenza type) to detect presence of influenza A and/or influenza B and PDZ domains in combination with panspecific antibodies to influenza A to distinguish pathogenic and seasonal influenza A subtypes. | 04-15-2010 |
20100092945 | TEST STRIP FOR A LATERAL FLOW ASSAY FOR A SAMPLE CONTAINING WHOLE CELLS - A test strip for conducting a lateral flow assay for detection of an analyte in a sample that contains cells and fluid is provided, as well as methods for making and using the test strip. This test strip is commercially usable, for example, in a diagnostic device at a point of care for detection of analytes in whole blood. | 04-15-2010 |
20100099076 | SENSITIVE AND RAPID DETECTION OF VIRAL PARTICLES IN EARLY VIRAL INFECTION BY LASER TWEEZERS - The present system and methods allow for low level detection of as little as single pathogen particles, such as viral or bacterial particles, during the early stage of infection. An optical trapping system, such as laser tweezers, are used to trap a substrate to which an analyte has been bound to detect and record the thermal motion of an antibody-antigen interaction that may occur between an anti-viral antibody-coated microsphere and a viral particle for example. The system may be equipped with a detection system such as a position sensitive photodetector (PSD) to record the thermal motion of a trapped microsphere and particle at a certain frequency. The thermal motion data may be Fourier transformed into a power spectrum, which may be transformed into an output value using a Lorentzian equation. The power spectrum of the trapped microsphere may be recorded before and after binding of the pathogenic particle to determine the presence thereof. | 04-22-2010 |
20100099077 | STABLE ACRIDINIUM ESTERS WITH FAST LIGHT EMISSION - Chemiluminescent acridinium esters are provided which are fast light emitting and hydrolytically stable. The chemiluminescent acridinium esters are useful labels in assays for detecting or quantifying analytes. | 04-22-2010 |
20100099078 | METHOD FOR DESIGNING A DRUG REGIME FOR HIV-INFECTED PATIENTS - The instant disclosure describes a novel genotype and phenotype assay to elucidate and/or evaluate new potential HIV integrase inhibitors, but also currently approved and experimental compounds that target protease, reverse transcriptase, and RNaseH. This assay allows studying linked mutations and mutational patterns that occur under HAART and experimental therapies. | 04-22-2010 |
20100099079 | Non-dividing cell-based assay for high throughput antiviral compound screening - The present invention features a cell-based assay that recapitulates all aspects of a viral lifecycle for use in identifying antiviral agents. The assay employs synchronized, non-dividing host cells and a fluorescence resonance energy transfer peptide substrate for monitoring endogenous viral protease activity, which is indicative of viral infection kinetics. | 04-22-2010 |
20100105024 | RAPID TEST INCLUDING GENETIC SEQUENCE PROBE - A rapid test kit may have a genetic probe, and antibody detecting probe or a combination of a genetic probe and an antibody detecting probe disposed within one or more test windows of the test kit. A cellulose filter paper membrane with a flow rate selected in a range of about 0.04 to about 0.4 ml/min/cm | 04-29-2010 |
20100105025 | DEVICES FOR GENERATING DETECTABLE POLYMERS - This document provides systems, devices, and methods involved in generating detectable polymers. For example, diagnostic systems, diagnostic devices, primer systems, and collections of primer systems are provided. | 04-29-2010 |
20100105026 | Optical measurement method for molecular detection using relaxation measurement in optically anisotropic nanoparticles and device for performing the method - Optical measurement methods are described that are suitable for determining the relaxation behavior of nanoparticles dispersed in a solution. The particles have optically anisotropic properties and are alignable by an external stimulus, for example, an electric or magnetic field. In this manner the optical detection of certain molecules that can bind specifically to the surface of the nanoparticles and thus change the relaxation behavior of the nanoparticles as well as to provide devices for carrying out the methods is possible. | 04-29-2010 |
20100105027 | DETECTION OF NUCLEIC ACIDS FROM MULTIPLE TYPES OF HUMAN PAPILLOMAVIRUSES - Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed. | 04-29-2010 |
20100112545 | TRANS-1,2-DIPHENYLETHLENE DERIVATIVES AND NANOSENSORS MADE THEREFROM - Novel trans-1,2-diphenylethylene derivatives are synthesized which can be used to form nanoparticles-monomer-nanomolecule-receptor nanosensors. These trans-1,2-diphenyl-ethylene derivatives are soluble in both water and organic solvents, highly fluorescent and can be synthesized in high yields. The trans-1,2-diphenylethylene derivatives are bonded to a nanoparticle, a nanomolecule bonded to the derivative and a receptor bonded to the nanomolecule to form a nanosensor that can be used to detect chemical and biological agents. | 05-06-2010 |
20100112546 | NANOSCALE SENSORS - Various aspects of the present invention generally relate to nanoscale wire devices and methods for use in determining analytes suspected to be present in a sample, and systems and methods of immobilizing entities such as reaction entities relative to nanoscale wires. In one aspect, a nucleic acid, such as DNA, may be immobilized relative to a nanoscale wire, and in some cases, grown from the nanoscale wire. In certain embodiments, the nucleic acid may interact with entities such as other nucleic acids, proteins, etc., and in some cases, such interactions may be reversible. As an example, an enzyme such as telomerase may be allowed to bind to DNA immobilized relative to a nanoscale wire. The telomerase may extend the length of the DNA, for instance, by reaction with free deoxynucleotide triphosphates in solution; additionally, various properties of the nucleic acid may be determined, for example, using electric field interactions between the nucleic acid and the nanoscale wire. In another aspect, the invention provides systems and methods for attaching entities such as nucleic acids, receptors such as gangliosides, or surfactants to a nanoscale wire, for example, using aldehyde-producing reactions or hydrophobic interactions. In some aspects, certain systems and methods of the present invention may be used to determine an analyte suspected to be present in a sample, for example, a toxin, a virus, or a small molecule. Systems and methods of using such nanoscale wires are disclosed in other aspects of the invention, for example, within a microarray. Still other aspects of the invention include assays, sensors, kits, and/or other devices that include such nanoscale wires, methods of making and/or using functionalized nanoscale wires (for example, in drug screening or high-throughput screening), and the like. | 05-06-2010 |
20100112547 | METHODS AND COMPOSITIONS FOR DIAGNOSIS AND TREATMENT OF INFLUENZA - The invention provides method and compositions for determining the presence and amount of an influenza virus in a sample including high risk strains of Influenza A. Also provided are methods for determining whether a subject is infected with a influenza virus, as well as, the type and strain of the influenza virus. The methods involve contacting a sample from the subject with a PDZ polypeptides (PDZ) and/or PDZ ligands (PL) and determining whether binding interactions occur between PDZ and PL. Assays for identifying anti-viral agents are also provided, as well as, methods for using the compositions to alter PDZ binding to PL in influenza infected cells. | 05-06-2010 |
20100112548 | KIT FOR DETECTING NON-PATHOGENIC OR PATHOGENIC INFLUENZA A SUBTYPE H5 VIRUS - Current methods for detecting influenza A subtype H5 virus, for example cell culture, haemagglutination-inhibition, fluorescent antibody and enzyme immunoassay, and reverse transcriptase polymerase chain reaction (RT-PCR) may have the disadvantages of low sensitivity and low specificity. Furthermore, such methods are relatively difficult to use, and may not be suitable for routine detection on a daily basis. The kit for detecting H5 virus of this invention may provide a user-friendly alternative that is relatively more sensitive and specific to H5 virus. The detection kit utilizes two specially designed primers A and B for the replication of H5 virus, and a specific capture probe for immobilizing the amplified viral RNA. An additional primer C is also designed for the detection of pathogenic H5 virus. The detection of H5 virus by the detection kit may be accomplished within one day if desired. | 05-06-2010 |
20100112549 | Microorganism Detection Method and Apparatus - Embodiments of the present invention relate to selective organism detection, and, more particularly to recombinant bacteriophages and the use of such recombinant bacteriophages to detect target bacteria and to detect specific nucleic acid sequences within said target bacteria thus allowing for the detection of phenotypic characteristics of said bacteria such as determining drug(s) to which such target bacteria are resistant. The present invention further relates to sample preparation apparatuses for preparing samples for detection and analysis using bacteriophage-based techniques, that are low in cost, easy to use, and do not require technical expertise or any additional laboratory infrastructure to perform. | 05-06-2010 |
20100112550 | Microfluidic Assay for Characterization of the Leukocyte Adhesion Cascade - An apparatus and method for identifying and screening for agents affecting the leukocyte adhesion cascade (LAC) encompassing rolling, adhesion and migration comprises an optically clear, plastic microfluidic chip comprising flow channels with diameters in the range of 10-500 μm. The flow channels are coated with endothelial cells and at least a portion of the flow channels contains 1-30 μm sized openings, optionally filled with a native or synthetic extracellular matrix, that allow leukocyte migration into one or more tissue spaces. | 05-06-2010 |
20100120016 | METHODS AND SYSTEMS FOR DETECTION OF CONTAMINANTS - An impedance biosensor for detecting a contaminant in a starting material, the biosensor comprising a housing, an input device supported by the housing, an output device supported by the housing, a microfluidic cell supported by the housing, the starting material being engagable with the microfluidic cell, and an impedance analyzer supported by the housing and operable to measure impedance of the starting material to detect the presence of a contaminant. | 05-13-2010 |
20100120017 | RAPID IMMUNE CHROMATOGRAPHIC DETECTION BY AMPLIFICATION OF THE COLLOIDAL GOLD SIGNAL - The present invention relates to a rapid immunochromatographic test device suitable to detect more than one target in a single assay at the same time, uses of said device for detecting diseases in a sample, a method for the production of said device as well as a kit comprising the device. | 05-13-2010 |
20100120018 | Integrated Active Flux Microfluidic Devices and Methods - The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules. | 05-13-2010 |
20100120019 | Detection, screening, and diagnosis of HPV-associated cancers - Embodiments of the invention provide methods, polyclonal antibodies, monoclonal antibodies, assays, and kits for detecting HPV infection, including infection by various HPV genotypes, early and/or late HPV-associated or HPV-specific proteins or antibodies. Monoclonal antibodies are used to detect oncogenic high risk and low risk HPV types in a single assay, which is not limited to assay type or format. Useful tools for specific detection of various HPV associated cancers are provided. HPV associated cancer biomarkers are identified and can be used in a screening method for early stage precancerous lesions as well as late stage cancer progression. | 05-13-2010 |
20100120020 | FLUORESCENT NEUTRALIZATION AND ADHERENCE INHIBITION ASSAYS - The present invention comprises rugged, inexpensive, reliable, and sensitive laboratory assays of antibody-based viral neutralization activity and antibody-based viral adherence inhibition activity. The assays use inactivated, fluorescently-labeled virus, allowing the tests to be performed without extensive safety precautions. The interaction of the labeled virus with target cells is monitored using flow cytometric methods. A preferred embodiment uses simple and inexpensive flow cytometry methodologies and equipment, such as bead array readers used as simplified flow cytometers. The assays are rapid, taking no longer than a few hours and are readily conducted by a trained technician. The assays are sensitive because they use labeled viruses at low concentrations and determine neutralizing and blocking capacity of sera and antibody at low concentrations. The methods are appropriate for high-throughput screening of large panels of samples. | 05-13-2010 |
20100120021 | HPV E6, E7 MRNA Assay and Methods of Use Thereof - Provided is an HPV E6, E7 mRNA assay, referenced herein as the “In Cell HPV Assay,” that is capable of sensitive and specific detection of normal cervical cells undergoing malignant transformation as well as abnormal cervical cells with pre-malignant or malignant lesions. The In Cell HPV Assay identifies HPV E6, E7 mRNA via in situ hybridization with oligonucleotides specific for HPV E6, E7 mRNA and quantitates the HPV E6, E7 mRNA via flow cytometry. The In Cell HPV Assay can be carried out in less than three hours directly from liquid-based cervical (“LBC”) cytology specimens. The In Cell HPV Assay provides an efficient and highly sensitive alternative to the Pap smear for determining abnormal cervical cytology. | 05-13-2010 |
20100129785 | AGENTS AND METHODS FOR SPECTROMETRIC ANALYSIS - Disclosed herein are agents, methods, and kits for determining the presence or concentration of a target, or multiple targets, in a sample, in a uniplexed or multiplexed fashion. In general, the methods enable the analysis of small molecules produced or consumed in liquid-phase that may be analyzed using gas or vapor phase detection methods. | 05-27-2010 |
20100129786 | AGENTS AND METHODS FOR SPECTROMETRIC ANALYSIS - Disclosed herein are agents, methods, and kits for determining the presence or concentration of a target, or multiple targets, in a sample, in a uniplexed or multiplexed fashion. In general, the methods enable the analysis of small molecules produced or consumed in liquid-phase that may be analyzed using gas or vapor phase detection methods. | 05-27-2010 |
20100129787 | AGENTS AND METHODS FOR SPECTROMETRIC ANALYSIS - Disclosed herein are agents, methods, and kits for determining the presence or concentration of a target, or multiple targets, in a sample, in a uniplexed or multiplexed fashion. In general, the methods enable the analysis of small molecules produced or consumed in liquid-phase that may be analyzed using gas or vapor phase detection methods. | 05-27-2010 |
20100129788 | METHOD FOR EVALUATING THE RESPONSE OF AN INDIVIDUAL TO A TREATMENT WITH A TYPE I INTERFERON (IFN) - A method for evaluating the in vivo presence of a factor that prevents the biological effect of a type I (IFN) in an individual that is under treatment with type I interferon is described. The in vivo presence of antibodies directed against a type I interferon (IFN) is evaluated in an individual that is under treatment with type I interferon. The method includes incubating a blood sample of the individual in vitro with a suitable amount of the type I interferon for a suitable period of time, and determining mRNA levels of a biological marker of IFN activity, preferably M×A, in the blood sample. The treatment may involve a treatment of multiple sclerosis, HCV or HBV using a type I interferon. | 05-27-2010 |
20100129789 | Automated assay and system - An automated assay processing method including transferring a first number of samples from respective sample containers to a first intermediary vessel, determining the testing adequacy of a second number of samples in a second intermediary vessel, preparing a third number of samples in a third intermediary vessel for downstream testing; and transferring a fourth number of samples from a fourth intermediary vessel to an output sample tray. These steps are all performed essentially simultaneously within the duration of a single clock cycle and are repeated during one or more subsequent clock cycles. The clock cycle may be relative to each intermediary vessel. The clock cycle also may be universal to the first, second, third and fourth intermediary vessels. | 05-27-2010 |
20100136513 | Assay for sars coronavirus by amplification and detection of nucleocapsid rna sequence - Primers and probes derived from SARS-CoV nucleic acid that facilitate detection and/or quantification of the nucleocapsid gene are disclosed. The disclosed sequences may be used in a variety of amplification and non-amplification formats for detection of SARSCoV infection. | 06-03-2010 |
20100136514 | INFECTIOUS CLONE OF HUMAN PARVOVIRUS B19 AND METHODS - The invention relates to infectious clones of parvovirus B19, methods of cloning infectious B19 clones, and methods of cloning viral genomes that have secondary DNA structures that are unstable in bacterial cells. A B19 infectious clone and methods of producing B19 infectious clones are useful for producing infectious virus. Infectious virus is useful for identifying and developing therapeutically effective compositions for treatment and/or prevention of human parvovirus B19 infections. | 06-03-2010 |
20100136515 | COMPOSITIONS FOR USE IN IDENTIFICATION OF PAPILLOMAVIRUS - The present invention relates generally to identification of HPV, and provides methods, compositions and kits useful for this purpose when combined, for example, with molecular mass or base composition analysis. | 06-03-2010 |
20100136516 | System and method for detection of HIV integrase variants - An embodiment of a method for detecting low frequency occurrence of one or more HIV sequence variants associated with integrase is described that comprises the steps of: (a) generating a cDNA species from a plurality of RNA molecules in an HIV sample population; (b) amplifying a plurality of first amplicons from the cDNA species, wherein each first amplicon is amplified with a pair of nucleic acid primers; (c) clonally amplifying the amplified copies of the first amplicons to produce a plurality of second amplicons; (d) determining a nucleic acid sequence composition of the second amplicons; (e) detecting one or more sequence variants that occur at a frequency of 5% or less in the nucleic acid sequence composition of the second amplicons; and (f) correlating the detected sequence variants with variation associated with HIV integrase. | 06-03-2010 |
20100136517 | MATRIX STABILIZATION OF AGGREGATION-BASED ASSAYS - Methods and apparatus for stabilization of aggregation-based assays are described. In various embodiments, anti-analytes are dispersed within a matrix. A solution containing analytes brought into contact with the matrix, so that analytes may permeate throughout at least a portion of the matrix. In some embodiments, the anti-analytes and analytes are mobile within the matrix. As aggregates form and increase in size, the aggregates become substantially immobile within the matrix. As a result, signals representative of an amount of aggregation within the matrix can remain substantially constant. In various aspects, matrix-stabilized aggregation-based assays provide for reliable quantitative analysis of analyte concentration with test solutions. | 06-03-2010 |
20100136518 | ANTIBODIES USEFUL FOR THERAPY AND DIAGNOSIS OF CANCER - The present invention provides antibodies, or fragments thereof, for isolating and/or identifying epitopes of an endogenous retrovirus, preferably of a melanoma associated endogenous retrovirus, and hybridoma cells producing said antibodies. The antibodies are useful especially for the treatment and diagnosis of cancer. Further, the present application covers diagnostic kits for the detection of cancer cells, especially of melanoma cells and methods for cancer diagnosis using said antibodies. | 06-03-2010 |
20100136519 | Determination of a specific immunoglobulin using multiple antigens - The invention concerns a process for the immunological determination of a specific antibody in a fluid sample. The process involves incubating the fluid sample in the presence of a solid phase with two antigens directed against the antibody whose presence is to be determined; the first antigen carries at least one marker group, while the second is either (a) bonded to the solid phase or (b) present in a form in which it can bond with the solid phase, and betrays the presence of the antibody being sought by showing the presence of the marker group in the solid phase and/or in the fluid phase. The proposed process is characterised by the fact that at least one of the two antigens has several epitopic regions which react with the antibody to be determined. | 06-03-2010 |
20100136520 | DETECTING HEPATITIS B VIRUS - This invention is related to methods for detecting hepatitis B virus by determining the level of hepatitis B virus surface antigen protein. This invention is also related to methods for detecting mutant hepatitis B virus surface antigen protein and kits for detecting hepatitis B virus. | 06-03-2010 |
20100136521 | Devices And Methods For Detection Of Microorganisms - The present invention features methods and devices for microorganisms through detecting Mie light scattering from immunoagglutinated beads. The methods feature providing a first bead suspension with antibody specific for the microorganism conjugated to beads; mixing the first bead suspension with a sample to form a first mixture; irradiating the first mixture with first incident light; detecting forward light scattering at a first angle with respect to the first incident light, where the first angle being between about 30 to 60 degrees; determining l from the light scattering; providing a second bead suspension with no antibody and simultaneously measuring l | 06-03-2010 |
20100143881 | Selective detection of human rhinovirus - A process for detecting human rhinovirus nucleic acid in a biological sample, includes producing an amplification product by amplifying an human bocavirus nucleotide sequence using a forward primer of SEQ ID NO: 1, and a reverse primer of SEQ ID NO: 2, and measuring said amplification product to detect human rhinovirus in said biological sample. Also provided are reagents and methods for detecting and distinguishing human rhinovirus from other viruses. A kit is provided for detecting and quantifying human rhinovirus in a biological sample. | 06-10-2010 |
20100143882 | IMPROVED MULTIPLEX NUCLEIC ACID AMPLIFICATION USING BLOCKED PRIMERS - The present invention is in the field of nucleic acid amplification, and in particular in transcription-based amplification, providing improvements thereof. Specifically, the present invention provides primers, and methods for using them, that improve transcription-based amplification reactions, in particular multiplex reactions. | 06-10-2010 |
20100143883 | CAPTURE OF MYCOBACTERIA LIKE MICRO-ORGANISMS - A method for the capture from a sample of micro-organisms having a hydrophobic surface, which method includes contacting the micro-organisms with a capture reagent, which capture reagent has both a hydrophobic character whereby the capture reagent binds the micro-organisms by hydrophobic interaction therewith and a polar character, the capture reagent either being present on a surface and capturing the micro-organisms thereto, or being present in solution, the method then further including capturing the micro-organisms to a surface by binding the capture reagent to the surface by polar interaction between the surface and the capture reagent. | 06-10-2010 |
20100143884 | DETECTION OF INFLUENZA VIRUS - The present application describes methods for detecting influenza A and/or influenza B and/or distinguishing between pathogenic and seasonal influenza A subtypes. Many of these preferred formats employ pan-specific antibodies (i.e., that react with all or at least multiple strains within an influenza type) to detect presence of influenza A and/or influenza B and PDZ domains in combination with panspecific antibodies to influenza A to distinguish pathogenic and seasonal influenza A subtypes. | 06-10-2010 |
20100143885 | DETECTION OF HUMAN PAPILLOMAVIRUS - There is provided an in vitro method of detecting human papillomavirus nucleic acid in a sample, comprising: (a) contacting said sample with forward and reverse oligonucleotide primers, wherein said primers bind to target sites in the human papillomavirus L1 gene, or the complement thereof, under conditions suitable to promote amplification of a portion of said human papillomavirus L1 gene or complement, thereby generating an amplicon; (b) contacting said amplicon with a probe, wherein the probe binds to a target site within said amplicon; and (c) detecting binding of said probe to said amplicon; wherein said forward primer binds to a target site having the sequence SEQ ID NO: 1; and wherein said reverse primer binds to a target site having the sequence SEQ ID NO: 2. | 06-10-2010 |
20100143886 | IN VIVO HCV RESISTANCE TO ANTI-VIRAL INHIBITORS - HCV mutations emerged in chimpanzees treated with a N5B polymerase inhibitor (Compound A) or a NS3 protease inhibitor (Compound B). Short term treatment with Compound A was followed by the initial emergence of an HCV with a S282T polymerase mutation following treatment. Short term treatment with Compound B selected for HCV with a R155K or D168T protease mutation. | 06-10-2010 |
20100143887 | BIOSENSOR AND METHOD FOR DETECTING BIOMOLECULES BY USING THE BIOSENSOR - Provided are a biosensor and a method for detecting biomolecules by using the biosensor. The biosensor includes a detection unit and a fluid channel. The detection unit is disposed on a substrate and has a surface to which detection target molecules binding specifically to probe molecules are immobilized. The fluid channel is configured to provide an analysis solution containing the probe molecules to the detection target molecules. The probe molecules bind specifically to the target molecules and the detection target molecules. | 06-10-2010 |
20100143888 | ENZYMATIC DIAGNOSTIC TEST FOR SARS AND OTHER VIRAL DISEASES - The present invention is directed towards methods, compositions and kits for testing for a virus in a sample. The methods determine the presence of a viral enzyme by contacting the sample with a peptidal compound capable of being cleaved by the viral enzyme to form peptidal compound fragments. Detection of a peptidal compound fragment confirms the presence of the virus. | 06-10-2010 |
20100143889 | RHABDOVIRIDAE VIRUS PREPARATIONS - This document involves methods and materials related to obtaining Rhabdoviridae virus preparations. For example, methods and materials for obtaining large volume, high titer, high purity preparations of Rhabdoviridae viruses (e.g., VSV) with low or non-existent levels of contaminants are provided. | 06-10-2010 |
20100143890 | PEPTIDE COMPOUNDS FOR CAPTURING OR INHIBITING AVIAN INFLUENZA VIRUS AND APPLICATION THEREOF - Disclosed herein are peptide, particularly dipeptide compounds and the application thereof to the detection or inhibition of AI virus. The peptide compounds are more stable and easier to synthesize and store than are antibodies. In addition, having strong binding forces for the H5 protein of AI virus, the peptide compounds are useful as capturers or inhibitors of AI virus. | 06-10-2010 |
20100143891 | Method for the Rapid Diagnosis of Targets in Human Body Fluids - More particularly, the present invention relates to a method for the detection of a target, e.g. pathogen in a human body fluid wherein a body fluid sample is collected with a swab member. | 06-10-2010 |
20100143892 | USE OF RIBOZYMES IN THE DETECTION OF ADVENTITIOUS AGENTS - The present invention provides a method of detecting adventitious agents in a composition comprising a microorganism by using ribozyme-expressing indicator cells, as well as indicator cells useful in such detection. | 06-10-2010 |
20100151441 | Human Cytomegalovirus Latency Promoting Genes, Related Virus Variants and Methods of Use - Latency promoting genomic sequences from human cytomegalovirus (HCMV) and virus variants lacking function of one or more of the latency promoting genes are disclosed. Also disclosed are methods of using the altered viruses and latency promoting genes and their gene products for the production of vaccines and for identifying antiviral compounds. | 06-17-2010 |
20100151442 | METHOD FOR DETECTING EMERGING PANDEMIC INFLUENZA - A method for detecting emerging pandemic influenza strains is provided. RT-PCR is used to detect HPAI followed by pyrosequencing to detect codons defining human or avian influenza signatures. This method screens for avian influenza viruses containing mutations suspected of making the virus more infective or virulent to humans. | 06-17-2010 |
20100151443 | MICROFLUID SYSTEM AND METHOD TO TEST FOR TARGET MOLECULES IN A BIOLOGICAL SAMPLE - A system and method to test for the presence of target molecules in a biological test sample includes test molecules, a microfluidic chip, and irradiating and detection devices. The test molecules include bio-recognition molecules conjugable with the target molecules, and the corresponding conjugates. The microfluidic chip includes sample channels and flow focusing channels adjoining the sample channels. A buffer exiting from the focusing channels directs a single-file stream of the test molecules through one of the sample channels. The irradiating device delivers radiation for absorption by the test molecules in the single-file stream. After absorption, the test molecules emit fluorescence of a distinct fluorescent spectrum for each of the conjugates. The detection device monitors identifies the presence of the conjugates by monitoring for the distinct fluorescent spectrum. Thus, the test system and method identifies the presence of the target molecules in the test sample. | 06-17-2010 |
20100151444 | Detection method for human pappilomavirus (HPV) and its application in cervical cancer - Embodiments of the invention provide methods, assays, and kits for detecting HPV infection and HPV associated epithelial cell abnormalities, most notably those associated with pre-malignant and malignant epithelial cell lesions. Detection of HPV DNAs, genomes, and/or oncoproteins by nucleic acid hybridization assays and immunological assays can be used in early clinical screening for HPV infection and diagnosis for cervical cancer. The polypeptides, recombinant proteins, antibodies, nucleic acids, and various detection methods thereof are particularly useful for diagnosing carcinomas of the uterine cervix and those at risk of developing cervical cancer. | 06-17-2010 |
20100151445 | Multiplex Method for Detecting an Infection - The invention relates to a method for the in vitro diagnostic detection of an infection with a microorganism, comprising placing a biological sample, in a single assay receptacle, in the presence of particles, each carrying at least one specific detectable physical parameter, and belonging to at least two different groups, one of the groups carrying an anti-IgM capture antibody and the other group carrying a capture antigen derived from said microorganism. | 06-17-2010 |
20100151446 | METHOD FOR COUNTING AND SEGMENTING VIRAL PARTICLES IN AN IMAGE - The method is for intracellular counting and segmentation of viral particles in an image. An image is provided that has a plurality of items therein. A radius range of viral particles is determined. Round items in the image having a radius within the predetermined radius range are identified. Elliptical items that are formable from the predetermined radius range are determined. The round and elliptical items identified into groups are sorted. The viral particles among the round and elliptical items are identified. For example, the method may be used for intracellular counting and segmentation of siRNA treated human cytomegaloviral particles in TEM images. | 06-17-2010 |
20100151447 | METHOD FOR DOUBLE STAINING IN IMMUNOHISTOCHEMISTRY - The present invention relates to kits and methods for performing dual-staining immunohistochemistry (IHC) for the detection of specific cell populations in tissue samples containing heterogeneous populations of cells, which can be observed by a light microscope for co-localization of distinct pigments. The method includes providing a tissue sample comprising fixed cells; exposing the sample to first and second ligands that recognize different marker proteins found at the same cellular location, thereby forming a ligand-labeled sample; exposing the ligand-labeled sample to first and second labeling reagents, the first labeling reagent binding to the first ligand and the second labeling reagent binding to the second ligand, the first and second labeling reagents each forming distinct pigments; and identifying the number of cells that display only one particular pigment, or more than one pigment, by the different coloration of the cellular location labeled by the distinct pigment. | 06-17-2010 |
20100159439 | Method for combined sequential agent delivery and electroporation for cell structures and use thereof - Disclosed is a method for sequential delivery of agents to and/or into a cell structure, wherein an electrolyte-filled tube is provided together with a counter electrode, said tube is connected to a voltage or current generator, at least two agents are introduced in a discrete mode into the electrolyte solution contained in the tube, which is placed close to the cell structure, one agent at the time being transported through the tube to and/or into said cell structure in which a pore has been formed by application of an electric field focused on the cell structure, resulting in electroporation of the cell structure. Also different applications of the method is disclosed, e.g. us of the method in order to transfer cell-impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure. | 06-24-2010 |
20100159440 | METHODS FOR IDENTIFYING AGENTS THAT MODULATE p44 - The present invention provides methods for identifying evolutionarily significant polynucleotide and polypeptide sequences in human and/or non-human primates which may be associated with a physiological condition, such as enhanced resistance to HCV infection. The invention also provides methods for identifying evolutionarily significant polynucleotides with mutations that are correlated with susceptibility to diseases, such as BRCA1 exon 11. The methods employ comparison of human and non-human primate sequences using statistical methods. Sequences thus identified may be useful as host therapeutic targets and/or in screening assays. | 06-24-2010 |
20100159441 | MULTI-WAVELENGTH ANALYSES OF SOL-PARTICLE SPECIFIC BINDING ASSAYS - The present invention discloses methods for detecting the presence of a complex between a first reagent and a second reagent in solution. In particular, the invention provides methods for qualitative or quantitative detection of an analyte or its specific binding partner in complex biological samples. The invention further discloses algorithms using summary rate changes at selected wavelengths in the absorbance spectra of colloidal metal-labeled analytes or specific binding partners to identify intermolecular interactions between the analyte and its binding partner. | 06-24-2010 |
20100159442 | MAGNETIC RECOGNITION SYSTEM - Provided is a label for an analyte, which label is attached to a magnetic or magnetizable substance, the label comprising: (a) a recognition moiety for attaching the label to the analyte; and (b) a moiety for binding or encapsulating the magnetic or magnetizable substance; wherein the moiety for binding or encapsulating the magnetic or magnetizable substance comprises a metal-binding protein, polypeptide, or peptide. | 06-24-2010 |
20100159443 | DISEASE MODEL INCORPORATION INTO AN ARTIFICIAL IMMUNE SYSTEM (AIS) - The present invention relates to methods for preparing an artificial immune system. The artificial immune system comprises a cell culture comprising a three-dimensional matrix comprising lymphoid tissue, a three-dimensional matrix comprising epithelial and/or endothelial cells, and diseased cells. The artificial immune system of the present invention can be used for in vitro testing of vaccines, adjuvants, immunotherapy candidates, cosmetics, drugs, biologics and other chemicals. | 06-24-2010 |
20100159444 | DEVICES FOR THE DETECTION OF MULTIPLE ANALYTES IN A SAMPLE - The present invention relates generally to an assay for detecting and differentiating multiple analytes, if present, in a single fluid sample, including devices and methods therefore. | 06-24-2010 |
20100167264 | Quantitative analyte assay device and method - The present invention relates to an assay device and a method for using such for the quantitative determination of an analyte, based on a test strip, which contains a porous test membrane allowing for capillary flow of the analyte and complexes of the analyte, a porous upstream membrane in fluid connection with the test membrane and a porous downstream membrane in fluid connection with the test membrane, wherein the test membrane contains a test site having immobilized thereon a ligand capable of reacting with the analyte and binding such to the test site, and two standard band sites having immobilized thereon known high and low concentrations of a calibrator agent capable of reacting with a label conjugate and binding such to the standard sites, wherein the upstream membrane has a site for the application of a sample to be analyzed, and has a site downstream from the sample application site for depositing label conjugates capable of reacting with the analyte and label conjugates capable of reacting with the immobilized calibrator agents in the standard bands to provide a known label response in the standards bands, and the downstream membrane is capable of absorbing said sample and providing the capillary flow for the sample through the upstream and test membrane. | 07-01-2010 |
20100167265 | VACCINE AGAINST INFECTIOUS AGENTS HAVING AN INTRACELLULAR PHASE, COMPOSITION FOR THE TREATMENT AND PREVENTION OF HIV INFECTIONS, ANTIBODIES AND METHOD OF DIAGNOSIS - A vaccine for treating and/or preventing infectious diseases where the infectious agent has at least one intracellular phase in the host during its multiplication cycle, is disclosed. The vaccine comprises at least one cryptic epitope of a cellular element that is carried along by an intracellular infectious agent as it leaves the cell, and revealed by said infectious agent. A composition for treating and/or preventing HIV infections, antibodies to a peptide of interest, and a diagnostic method, are also disclosed. | 07-01-2010 |
20100167266 | Compositions and Methods for Determining Whether a Subject Would Benefit from Co-Receptor Inhibitor Therapy - The present invention provides methods and compositions for determining whether a subject would benefit from co-receptor inhibitor therapy. In certain aspects, the methods can be used to determine whether a subject infected with a dual-mixed tropic population of HIV would benefit from CCCR5-inhibitor therapy or CXCR4-inhibitor therapy, the methods comprising determining whether the HIV population is a homogeneous or heterogeneous population of HIV, wherein the nature of the homogenous or heterogenous population of HIV indicates whether the patient would benefit from co-receptor inhibitor therapy. | 07-01-2010 |
20100167267 | Mass Spectrometric Quantitation - Provided is a method of assaying for an analyte, which method comprises: a) combining a test sample, which may comprise the analyte, with a calibration sample comprising at least two different aliquots of the analyte, each aliquot having a known quantity of the analyte, wherein the sample and each aliquot are differentially labeled with one or more isobaric mass labels each with a mass spectrometrically distinct mass marker group, such that the test sample and each aliquot of the calibration sample can be distinguished by mass spectrometry; b) determining by mass spectrometry the quantity of the analyte in the test sample and the quantity of the analyte in each aliquot in the calibration sample, and calibrating the quantity of the analyte in the test sample against the known and determined quantities of the analytes in the aliquots in the calibration sample. | 07-01-2010 |
20100167268 | SEROCONVERSION ASSAYS FOR DETECTING XENOTROPIC MURINE LEUKEMIA VIRUS-RELATED VIRUS - Methods of detecting, diagnosing, monitoring or managing an XMRV-related disease such as an XMRV-related neuroimmune disease such as chronic fatigue syndrome or an XMRV-related lymphoma such as mantle cell lymphoma in a subject are disclosed. These methods comprise determining presence, absence or quantity of antibodies against XMRV in a sample from a subject. | 07-01-2010 |
20100167269 | Detection method for human pappilomavirus (HPV) and its application in cervical Cancer - Embodiments of the invention provide methods, assays, and kits for detecting HPV infection and HPV associated epithelial cell abnormalities, most notably those associated with pre-malignant and malignant epithelial cell lesions. Detection of HPV DNAs, genomes, and/or oncoproteins by nucleic acid hybridization assays and immunological assays can be used in early clinical screening for HPV infection and diagnosis for cervical cancer. The polypeptides, recombinant proteins, antibodies, nucleic acids, and various detection methods thereof are particularly useful for diagnosing carcinomas of the uterine cervix and those at risk of developing cervical cancer. | 07-01-2010 |
20100167270 | METAPNEUMOVIRUS STRAINS AND THEIR USE IN VACCINE FORMULATIONS AND AS VECTORS FOR EXPRESSION OF ANTIGENIC SEQUENCES AND METHODS FOR PROPAGATING VIRUS - The invention relates to improved strains of mammalian negative strand RNA virus, | 07-01-2010 |
20100167271 | METHOD FOR SCREENING BLOOD USING A PRESERVATIVE THAT MAY BE IN A SUBSTANTIALLY SOLID STATE FORM - Methods and devices useful for screening a blood product for a transfusion, pursuant to which leukocytes in drawn whole blood are contacted with a formaldehyde releaser screening preservative so that the presence of any residual leukocytes can be screened. A substantially solid state form preservative for one or more blood components (e.g., leukocytes) and use thereof is also described. | 07-01-2010 |
20100167272 | Method for Detecting and Analyzing Pathogens in a Sample - The present invention relates to a method and kits thereof for detecting the presence and/or the specific serogroup of a prokaryote selected from the group consisting of & | 07-01-2010 |
20100173279 | DETECTION USING PRIMERS TO REPETITIVE DNA AND TRANSCRIPTION-BASED AMPLIFICATION THEREBY - The present invention concerns identifying organisms based on detecting distinguishing patterns produced following RNA amplification that originates via a DNA template. In particular, the methods and compositions of the invention concern obtaining ds DNA from the organism in question, amplifying at least part of the DNA via RNA molecules from transcription using primers that target repetitive DNA, and detecting a hallmark pattern of the amplified RNA. | 07-08-2010 |
20100173280 | Systems for Detection and Production of Respiratory, Herpes and Enteric Viruses - The present invention generally relates to the field of diagnostic microbiology, and, more particularly, to compositions and methods for detecting and differentiating one or more viruses or other intracellular parasites present in a specimen. The present invention also provides compositions and methods to evaluate the susceptibility of organisms to antimicrobial agents. | 07-08-2010 |
20100173281 | HCV NS3 protease replicon shuttle vectors - The present invention provides for novel HCV NS3 protease replicon shuttle vectors useful for cloning in HCV polynucleotide sequences from samples of HCV-infected patients and testing the resulting replicons for drug susceptibility. | 07-08-2010 |
20100173282 | MUTATIONAL PROFILE IN HIV-1 GAG CLEAVAGE SITE CORRELATED WITH PHENOTYPIC DRUG RESISTANCE - The invention concerns novel mutations or mutational profiles of HIV-1 protease cleavage sites (CS) in the Gag region correlated with a phenotype causing alterations in sensitivity to anti-HIV drugs. The present invention also relates to the use of genotypic characterization of a target population of HIV and the subsequent association, i.e. correlation, of this information to phenotypic interpretation in order to correlate virus mutational profiles with drug resistance. The invention further relates to methods of utilizing the mutational profiles of the invention in databases, drug development, i.e., drug design, and drug modification, therapy and treatment design and clinical management. | 07-08-2010 |
20100173283 | METHOD OF PREDICTING POTENTIAL SEVERITY OF HEPATITIS E, PROBE SETS, AND PRIMER SETS - The present invention provides a method of predicting potential severity of hepatitis, includes determining that hepatitis in a subject is potentially severe when it is detected that any amino acid is mutated to an amino acid of genotype-4, the any amino acid being amino acid of an amino acid sequence in a region encoded by ORF1 of an HEV genome RNA of genotype 3, and the HEV genome RNA being contained in a specimen nucleic acid taken from the subject infected with genotype-3 HEV. | 07-08-2010 |
20100173284 | Method for Detection of HCV at the Real Time PCR with Intercalating Dye - The present invention relates to a composition for detection of HCV by a single step reaction, comprising a specific primer and probe. In particular, the present invention relates to a composition for detection of HCV by a single step reaction, comprising the primer sequences of SEQ ID NOs:1 and 2; a composition for detection of HCV by a single step reaction, comprising both the primer sequences and a probe of SEQ ID NOs:5 or 9; a method for detecting HCV by a single step reaction, comprising the steps of obtaining a sample from a subject, and amplifying and detecting HCV using the primer and probe; and a kit comprising the primer and probe, in which the HCV detection method is characterized by a single step reaction. | 07-08-2010 |
20100184014 | Non-M, non-O HIV-1 strains, fragments and uses - Retroviral strains of the non-M, non-O HIV-1 group, in particular a strain designated YBF30, its fragments and also its uses as a diagnostic reagent and as an immunogenic agent. | 07-22-2010 |
20100184015 | METHOD FOR DETECTION OF XMRV - The present invention relates to the identification of Xenotropic murine leukemia virus (XMRV) nucleic acid by polymerase chain reaction (PCR) analysis (e.g., real time PCR (RT/PCR); nested RT/PCR using Tth DNA polymerase and Hot start polymerase) and the uses thereof. In particular, the invention provides methods for the detection, and in particular early detection, of XMRV in RNA isolated from samples (e.g., urine samples; expressed prostate secretion (EPS)) of prostate cancer patients and normal individuals. | 07-22-2010 |
20100184016 | METHODS AND COMPOSITIONS IN THE TREATMENT OF PORCINE CIRCOVIRAL INFECTION - The invention relates generally to the field of virology. More particularly, the present invention relates to methods of diagnosing, prognosis, treatment and prevention of porcine circoviral infection in mammals, in particular of porcine circovirus type 2 (PCV2). | 07-22-2010 |
20100184017 | DIFFERENTIAL AMPLIFICATION OF MUTANT NUCLEIC ACIDS BY PCR IN A MIXTURE OF NUCLEIC ACIDS - A method for enriching a mutant nucleic acid in a mixture of nucleic acids, wherein the method comprises: (a) providing a nucleic acid mixture comprising a parental nucleic acid and a mutant nucleic acid of the parental nucleic acid; and (b) amplifying the nucleic acids in the nucleic acid mixture by polymerase chain reaction (PCR); wherein the mutant nucleic acid is a G→A mutant of the parental nucleic acid, which pairs with a fully complementary nucleic acid sequence to form an AT-rich nucleic acid variant of the parental nucleic acid; and wherein the AT-rich nucleic acid variant is denatured and selectively amplified by carrying out PCR using a denaturation temperature 1-3° C. lower than the lowest denaturation temperature (T | 07-22-2010 |
20100184018 | Detection of Herpes Simplex Virus - The invention provides methods to detect herpes simplex virus (HSV) in biological samples and further to distinguish between HSV-1 and HSV-2. Primers and probes for the differential detection of HSV-1 and HSV-2 are provided by the invention. Articles of manufacture containing such primers and probes for detecting HSV are further provided by the invention. | 07-22-2010 |
20100184019 | Novel Human Virus Causing Severe Acute Respiratory Syndrome (SARS) and Uses Thereof - The present invention relates to an isolated novel virus causing Severe Acute Respiratory Syndrome (SARS) in humans (“hSARS virus”). The hSARS virus is identified to be morphologically and phylogenetically similar to known member of Coronaviridae. The present invention provides the complete genomic sequence of the hSARS virus. Furthermore, the invention provides the nucleic acids and peptides encoded by and/or derived from the hSARS virus and their use in diagnostic methods and therapeutic methods, including vaccines. In addition, the invention provides chimeric or recombinant viruses encoded by said nucleotide sequences and antibodies immunospecific to the polypeptides encoded by the nucleotide sequences. | 07-22-2010 |
20100190147 | METHOD OF DETERMINING IMMUNE ENHANCEMENT OF VIRUS INFECTIVITY USING FC RECEPTOR-TRANSFECTED CELL LINES - The present invention relates to a method of detecting immune enhancement of virus infectivity, a method of determining neutralization and immune enhancement of virus infectivity, a method of identifying a virus epitope that displays immune enhancement, a method of identifying a compound that modulates activity of an Fc receptor, and a method of identifying a compound that modulates intracellular signaling of an Fc receptor. DNA constructs, cells, and kits relating to these assays are also disclosed. | 07-29-2010 |
20100190148 | Electrophoretic Interactive Spectral Methods and Devices for the Detection and/or Characterization of Biological Particles - Methods for identifying a biological particle in a sample medium include generating an Electrophoretic Quasi-elastic Light Scattering (EQELS) spectrum for the biological particle in the sample medium. The EQELS spectrum is compared to a reference database comprising a plurality of spectra, and each of the plurality of spectra correspond to an EQELS spectrum for one of a plurality of known biological particles. The biological particle in the sample medium is identified from the comparison. | 07-29-2010 |
20100190149 | CROSS-REACTIVE HYBRIDIZATION PROBE FOR DETECTING HIV-1 AND HIV-2 NUCLEIC ACIDS IN THE p31 GENE SEQUENCE - Cross-reacting hybridization probe for detecting HIV-1 and HIV-2 nucleic acids. The probe advantageously exhibited uniformly high signal-to-noise ratios when hybridized to HIV-1 and HIV-2 target nucleic acids. The probe can be used, for example, in screening applications for detecting donated blood contaminated with either of the two analytes. | 07-29-2010 |
20100196874 | METHOD OF DRUG DESIGN - The description discloses that amiloride-like compounds inhibit enterovirus RNA replication by interaction with RNA dependent RNA polymerase (RdRP). The description discloses in silico and in vitro methods of screening for inhibitors of RdRP activity, amiloride-resistant enterovirus variants and amiloride-like compounds. | 08-05-2010 |
20100196875 | HUMAN ERYTHROVIRUS - Nucleic acid molecules derived from sequences of novel human parvovirus B19 variant genomes are provided. Also provided are assays and kits comprising the nucleic acid molecules. | 08-05-2010 |
20100196876 | Methods and Reagents for Genotyping HCV - The present invention is directed to methods and reagents for determining the genotype of a hepatitis C virus (HCV) species present in a test sample. The invention more particularly relates to mixtures of degenerate amplification and sequencing primers, and methods of using such primers, that are complementary to a plurality of HCV species, and are capable of generating nucleotide sequence information for a region of NS5B of HCV that is, for each species, indicative of the type and/or subtype, of the species present in the sample. | 08-05-2010 |
20100196877 | METHOD OF DETECTION OF MICROORGANISMS WITH ENHANCED BACTERIOPHAGE AMPLIFICATION - A method of determining the presence or absence of a target microorganism in a sample to be tested, the method comprising: combining with the sample an amount of bacteriophage capable of attaching to the target microorganism to create a bacteriophage exposed sample, and a substance which enhances bacteriophage amplification or sensitivity; providing conditions to the bacteriophage-exposed sample sufficient to allow the bacteriophage to infect the microorganism; and assaying the bacteriophage-exposed sample to detect the presence or absence of a bacteriophage marker to determine the presence or absence of the target microorganism. | 08-05-2010 |
20100196878 | DUAL-RECOGNITION IMMUNOASSAY FOR THE DETECTION OF ANTIBODIES - The invention relates to a dual-recognition immunoassay for the detection of antibodies specific to a target antigen in a sample which comprises contacting said target antigen with a sample suspected of containing said antibodies specific to said target antigen under conditions allowing the formation of an antigen-antibody complex; adding a conjugate comprising said target antigen and a marker under conditions allowing the formation of an antigen-antibody-antigen/marker complex; and detecting said antigen-antibody-antigen/marker complex. The immunoassay can be used, among other applications, in the diagnosis of infections caused by pathogenic organisms with high sensitivity and specificity. | 08-05-2010 |
20100203496 | Fluorescent Multiplex HPV PCR Assays - The present invention relates a fluorescent multiplex PCR assay for detecting the presence of a nucleic acid sequence of an HPV type in a sample using multiple fluorophores to simultaneously detect a plurality of HPV genes of the same HPV type, wherein the HPV type is selected from the group consisting of: HPV31, HPV45, HPV52, and HPV58. The present invention also relates to oligonucleotide primers and probes specific to said HPV types for use in the methods of the present invention. | 08-12-2010 |
20100203497 | SYSTEM AND METHOD FOR DETECTION OF HIV DRUG RESISTANT VARIANTS - In one embodiment of the invention a method for detecting low frequency occurrence of one or more HIV sequence variants associated with drug resistance is describe that comprises generating cDNA species from RNA molecules in an HIV sample population; amplifying first amplicons from the cDNA species, wherein each amplicon comprises amplified copies and is amplified with a pair of nucleic acid primers that define a locus; clonally amplifying the amplified copies of the first amplicons to produce second amplicons that comprise an immobilized population of substantially identical copies from one of the amplified copies of first amplicons; determining a nucleic acid sequence composition from at least 100 of the immobilized populations in parallel on a single instrument; detecting one or more sequence variants that occur at a frequency of 5% or less in the nucleic acid sequence composition of the at least 100 immobilized populations; and correlating the detected sequence variants with variation associated with HIV drug resistance. | 08-12-2010 |
20100203498 | METHODS AND COMPOSITIONS FOR IDENTIFYING ANTI-HCV AGENTS - The invention provides methods and compositions for identifying agents for treating infection by viruses that encode a nucleotide-binding NS4B protein, or functional equivalent thereof, e.g., hepatitis C virus (HCV) or other members of the family Flaviviridae. In general, the methods involve contacting an NS4B nucleotide binding motif (NBM)-containing polypeptide with a candidate agent, and determining the effect of the candidate agent on nucleotide binding activity, a nucleotide hydrolyzing activity, or a nucleotide-dependent RNA binding activity of the polypeptide. A candidate agent that inhibits NS4B polypeptide binding to a nucleotide is an anti-viral agent, e.g., an anti-HCV agent. The invention also features a polynucleotide encoding a NS4B polypeptide having a modified NBM (e.g., which is impaired in NTP binding). The subject methods and compositions find use in a variety of therapeutic and screening applications. | 08-12-2010 |
20100203499 | Electrophoretic Interactive Spectral Methods and Devices for the Detection and/or Characterization of Biological Particles - Methods for identifying a biological particle in a sample medium include generating an Electrophoretic Quasi-elastic Light Scattering (EQELS) spectrum for the biological particle in the sample medium. The EQELS spectrum is compared to a reference database comprising a plurality of spectra, and each of the plurality of spectra correspond to an EQELS spectrum for one of a plurality of known biological particles. The biological particle in the sample medium is identified from the comparison. | 08-12-2010 |
20100203500 | METHOD FOR DETECTION OF HOP LATENT VIRUS, PRIMER SET FOR THE DETECTION, AND KIT FOR THE DETECTION - The invention provides a detection method for hop latent virus that comprises an extraction step in which hop latent virus RNA is extracted from a specimen, an amplification step in which a primer set containing 4 different oligonucleotides consisting of the nucleotide sequences represented by SEQ ID NO: 1-4 of the Sequence Listing is used for amplification of cDNA by RT-LAMP using the RNA as template, and a judging step in which hop latent virus is judged to be present when amplification of cDNA containing the nucleotide sequence represented by SEQ ID NO: 8 of the Sequence Listing has occurred in the amplification step. | 08-12-2010 |
20100203501 | Assay to detect HCV receptor binding - Identification of HCV receptor target cells using HCV receptor-binding ligands and cell separation by flow cytofluorimetry is described. HCV receptor target cells are employed to conduct assays for HCV receptor-binding ligands in order to identify potential HCV vaccine candidates. HCV receptor target cells are used to measure antibody neutralisation to monitor vaccine development, as a diagnostic of HCV infection and to develop neutralising antibodies for passive immunisation. | 08-12-2010 |
20100209903 | Method for measuring resistance of a patient HIV-2 to protease inhibitors - A search method in a biological sample containing an HIV-2 viral strain for possible resistance of said strain to treatment by an anti-protease agent, and nucleotide probes for the implementation thereof. According to methods known per se, the presence of at least one mutation at certain, specified, particular positions of the proteinic sequence of the protease of said viral strain from a biological sample taken from a patient contaminated by HIV-2 is searched. If said mutation is observed, the existence of a resistance to said anti-protease agent is assumed in the patient. | 08-19-2010 |
20100209904 | Antibodies for oncogenic strains of HPV and methods of their use - The subject invention provides antibodies, including polyclonal and monoclonal antibodies, that bind to E6 proteins from at least three oncogenic strains of HPV. In general, the antibodies bind to amino acids motifs that are conserved between the E6 proteins of different HPV strains, particularly HPV strains 16 and 18. The subject antibodies may be used to detect HPV E6 protein in a sample, and, accordingly, the antibodies find use in a variety of diagnostic applications, including methods of diagnosing cancer. Kits for performing the subject methods and containing the subject antibodies are also provided. | 08-19-2010 |
20100209905 | PROTEIN D - AN IGD-BINDING PROTEIN OF HAEMOPHILUS INFLUENZAE - A novel surface exposed protein of | 08-19-2010 |
20100216116 | VIRAL LATENCY MODEL - The invention relates generally to the field of virology. More particularly, the present invention relates to in vitro models for viral latency. In particular to latently infected cultures of primary and continuous cell lines, and to the use thereof in methods to identify anti-viral compounds. More in particular to identify compounds which are either able to modulate the induction of viral latency in the aforementioned cell cultures, or which are able to retain the viruses in the aforementioned cells in their latent form. Other aspects of the invention are directed to antiviral compounds identified using the models and methods of the present invention, as well as to the use thereof in treating latent infections such as for example latent Herpes Simplex Virus (HSV) infections. | 08-26-2010 |
20100216117 | OLIGONUCLEOTIDES, USE, METHOD OF DETECTION AND KIT FOR DIAGNOSING THE PRESENCE OF THE CHIKUNGUNYA VIRUS E1 GENE - The present invention concerns oligonucleotides intended to enable the amplification and the detection of a target sequence located in the E1 gene of the Chikungunya virus. These oligonucleotides are between 10 and 50 nucleotides in length and comprise at least one fragment of 10 consecutive nucleotides derived from the following sequences: SEQ ID No. 1: 5′-CTCTTACCGGGTTTGTTGC-3′ or SEQ ID No. 2: 5′-GCCTGGACACCTTTCGAC-3′, or the sequence complementary thereto. The invention also concerns the oligonucleotide which enables detection of the amplicons, the use of these oligonucleotides, a method of detection and a kit for diagnosing the presence of the E1 gene of the Chikungunya virus. The invention has a preferred use in the diagnostics field. | 08-26-2010 |
20100216118 | Method of Detection/Extraction, and Related Detection/Extraction Device - A method that uses an L-α-dipalmitoleoyl-phosphatidylcholine (DPPC) surfactant based device that reacts with a substance in a known manner, to detect a substance of interest or to extract a substance of interest from a material is provided. The principles of the present invention are particularly useful in detecting/measuring a substance that is harmful to a human, and also to extracting NACL from saltwater. | 08-26-2010 |
20100216119 | Diagnostic Methods for HIV Infection - The invention relates to a method of aiding the diagnosis of a human immunodeficiency virus infection in a subject, said method comprising (i) providing a sample from the subject (ii) determining the level of ps20 in said sample (iii) comparing the level of ps20 of (ii) with the level of ps20 in an uninfected reference sample, wherein a higher level of ps20 in the sample from the subject compared to the uninfected reference sample indicates an increased likelihood of human immunodeficiency virus infection in said subject. The invention also relates to methods for assessing susceptibility of a subject to human immunodeficiency virus infection. Most suitably the ps20 level is determined via binding by an anti-ps20 antibody such as the 107 antibody. The invention also relates to kits for use in said methods. | 08-26-2010 |
20100216120 | Rapid infectious virus assay - An assay to detect or quantify HIV infectious virus from clinically relevant cellular compartments, or reservoirs, in anti-retrovirally treated patients whose viral levels are low to undetectable is described. The method detects infectious virus in patients whose plasma viral loads are considered to be below the limit of current PCR based detection methods and thereby is more relevant for guiding treatment. A further advantage is that the method allows viral tropism to be directly determined in the presence of specific inhibitors of CCR5 or CXCR4. Drug sensitivity can also be directly determined without the need to laboriously recover patient virus by culture for extended time periods, a method that allows for viral selection or evolution, which is not desirable. Patient cells, like the blood mononuclear cells, or monocytes, are isolated and cultured in the presence of cytokines like CSF-1/M-CSF or GM-CSF. to promote their differentiation. Cells are activated with lectins, mitogenic antibodies, phorbol esters, Toll Receptor stimulation or inducers of NfKb or NFAT, followed by agents that induce viral release, like ATP or stimulation of autophagy with LiCl, spermidine, or rapamycin. A key aspect of the invention relates to the timing of the addition of these agents for optimal viral release. A further aspect of the invention relates to sensitive detection of released virus which can be accomplished by adding so-called reporter cells which are under control of the HIV TAT protein so that upon infection these cells synthesize proteins or enzymes that allow for the measurement of infectious particles. | 08-26-2010 |
20100221700 | METHOD OF MONITORING HIV INFECTION - The present invention relates, in general, to human immunodeficiency virus (HIV) and, in particular, to a method of monitoring the intensity of HIV infection and predicting the time to progression to acquired immunodeficiency syndrome (AIDS). | 09-02-2010 |
20100221701 | VERSATILE THERMAL DETECTION - The present application discloses a method of detecting hybridization of complementary segments of nucleic acids by a heat generated upon aforementioned hybridization, a method of detecting the presence of a predetermined reactant in a sample suspected of containing the same, a method of detecting complex responses occur in biological systems by a heat generated upon triggering the same and a method of detecting the presence of a predetermined explosive material; an apparatus for detecting hybridization of complementary segments of nucleic acids by a heat generated upon aforementioned hybridization, an apparatus for detecting the presence of a predetermined reactant in a sample suspected of containing the same, and an apparatus for detecting the presence of a predetermined explosive material; a system for detecting complex responses occur in biological systems by a heat generated upon triggering of aforesaid biological systems; implementing a pyroelectric thermal sensor or a bolometric thermal sensor or a quantum well thermal sensor. | 09-02-2010 |
20100227310 | FLOW CYTOMETRY METHODS AND IMMUNODIAGNOSTICS WITH MASS SENSITIVE READOUT - Mass cytometry method. In one aspect, the method includes providing a sample having at least one cell type and mixing the sample with material such as nanoparticles functionalized with affinity molecules for the at least one cell type. The sample is transported through a suspended microchannel resonator to record a mass histogram and a cell count for the at least one cell type is determined from the histogram. | 09-09-2010 |
20100227311 | TISSUE CULTURE SYSTEM FOR PRODUCTION OF HEPATITIS C VIRUS - A tissue culture system for production of infectious hepatitis C virus is described. In particular, the invention provides recombinant monocistronic and bicistronic genomic constructs for production of virus, including constructs for production of wild-type HCV type 2 | 09-09-2010 |
20100227312 | Particle Adhesion Assay for Microfluidic Bifurcations - A method for characterizing particle adhesion in microfluidic bifurcations and junctions comprises at least one idealized bifurcation or junction. Multiple bifurcations and/or junctions can be combined on a single microfluidic chip to create microfluidic networks configured for assays specifically to characterize particle interactions at junctions or to screen particles for desired interactions with microfluidic bifurcations and/or junctions. | 09-09-2010 |
20100227313 | Methods and Compositions for Determining Altered Susceptibility of HIV-1 to Protease Inhibitor Treatment - This invention relates to methods for determining altered susceptibility of HIV-I viruses to protease inhibitors (PIs) based on the viral genotypes. The methods generally comprise detecting, in a gene encoding protease and/or gag of the HIV-I, the presence of mutations correlated with altered susceptibility to amprenavir and/or darunavir. | 09-09-2010 |
20100227314 | METHOD OF IDENTIFICATION OF GENOTYPE AND SUBTYPE OF HEPATITIS C VIRUS ON A BIOLOGICAL MICROCHIP - The invention relates to molecular biology, virology and medicine and provides a method for identifying a genotype and a subtype of Hepatitis C virus (HCV) on the basis of the analysis of an HCV genome NS5B region using a differentiating biochip. The method of the present invention is based on a two-step PCR, with a fluorescent labeled, preferably single-stranded, NS5B region fragment obtaining, followed by the hybridization of this fragment on a biochip comprising a set of specific discriminating oligonucleotides. HCV genotype and subtype identification is carried out by defining the specific sequences of the segments of the NS5B region fragment. The invention allows one to conduct an assay precisely from a clinical specimen, to determine 6 genotypes and 36 subtypes of hepatitis C virus, including the most virulent and drug resistant forms, and to reduce the cost of assay. Also, the invention deals with a biochip, a design method and a set of oligonucleotide probes usable under the implementation of the method. | 09-09-2010 |
20100233674 | Cells for detection and production of influenza and parainfluenza viruses - The invention provides cell lines that are useful for the rapid detection and production of influenza and parainfluenza viruses. In particular, the invention relates to transgenic cells with increased sensitivity to infection by influenza A, influenza B, or parainfluenza 3 viruses, or which are capable of enhanced productivity of infectious virions. The invention is suitable for use in culturing clinical influenza and parainfluenza virus isolates and for the production of influenza and parainfluenza virus for vaccine formulations, as antigen preparations for diagnostic applications, and for screening antiviral drugs. | 09-16-2010 |
20100233675 | ANALYTE MANIPULATION AND DETECTION - Provided is a method for separating two or more analytes in a fluid, which method comprises: (a) binding each different analyte to a different functional particle in one or more binding zones, to produce two or more bound analytes; (b) allowing the bound analytes to move through a separating conduit to two or more separate functional zones; wherein, each different functional particle has, or can be controlled to have, a different function in the fluid as compared with the other functional particles; and wherein the separating conduit separates into two or more functional conduits, such that the separating conduit serves to separate the bound analytes into the separate functional conduits by means of the different functions of the different functional particles. Also provided is an apparatus for separating two or more analytes in a fluid, which apparatus comprises: (a) a binding zone; (b) two or more functional conduits; (c) a separating conduit connecting the binding zone to the two or more functional conduits; (d) a transporter for transporting the analyte through the separating conduit from the binding zone to the two or more functional conduits; and (e) optionally one or more concentrating zones in connection with at least one of the functional conduits. | 09-16-2010 |
20100233676 | HIGH AFFINITY FLUOROCHROME BINDING PEPTIDES - The present invention contemplates strategies comprising small molecule, cell permeable probes that allow site-specific protein labeling for visualizing biological processes. In one embodiment, the present invention contemplates a series of short peptide sequences comprising high affinity binding (i.e., for example, subnanomolar affinity (0.53 nM) for indocyanine fluorochromes. In one embodiment, the peptide sequences comprise a 5 pmol detection limit for indocyanine fluorochromes. In one embodiment, the present invention contemplates methods comprising high affinity peptide-fluorochrome binding pairs in biological applications including, but not limited to, enzyme linked immunoabsorbent assay (ELISA), fluorescence activated cell sorting (FACS), microscopy (i.e., for example scanning electromicroscopy), Western Blots, histochemistry, protein and cell based tracking both in vitro and in vivo. | 09-16-2010 |
20100233677 | FULL GENOME SEQUENCES OF HUMAN RHINOVIRUS STRAINS - Infection by human rhinovirus (HRV) causes upper and lower respiratory tract disease with varying degrees of virulence. The molecular basis of diversity was examined by completing the genome sequences for all known serotypes (n=99) as well as novel field samples. Superimposition of capsid crystal structure and optimal-energy RNA configurations established the alignments. The phylogeny revealed conserved motifs, Glade-specific diversity including a potential new species (clade-D), pan-genome mutations in field isolates, and unexpected recombination that contributes to heterogeneity. A spacer tract near a 5′-UTR cloverleaf was hypervariable, and in analogy with poliovirus, may be associated with virulence. A previously unidentified configuration consistent with non-scanning internal ribosome entry may account for rapid protein translation. The data density from complete sequences of the HRV reference serotypes provided high resolution for this degree of modeling, and serves as a platform for full genome-based epidemiologic studies, for viral diagnostics and prognostics, and for antiviral compounds and vaccines. | 09-16-2010 |
20100233678 | TUNABLE AFFINITY LIGANDS FOR THE SEPARATION AND DETECTION OF TARGET SUBSTANCES - Conformationally tunable affinity ligands are rationally designed and selected for the ability to switch under operator-defined environmental conditions between or among structurally distinct states that have different affinities for a given target substance. Tunable affinity ligands are incorporated into reagents, separation media, assays, sensors, devices, kits and systems for sorting, separating, detecting, sensing, quantifying, identifying and monitoring target substances. Applications include biomedical research, diagnostics, drug discovery, bioproduction and processing and environmental, industrial, chemical, agricultural and military use. | 09-16-2010 |
20100240023 | METHOD FOR EXTRACTING DEOXYRIBONUCLEIC ACIDS (DNA) FROM MICROORGANISMS POSSIBLY PRESENT IN A BLOOD SAMPLE - The present invention relates to a method for extracting DNA from microorganisms possibly present in a blood sample, comprising the following steps: i) filtration of a blood sample through a filtration membrane, the pores of which have a diameter ranging from 0.01 μm to 50 μm, in particular from 0.1 μm to 10 μm, and most particularly from 0.2 μm to 1 μm; ii)) washing of said filtration membrane; and iii) extraction of the deoxyribonucleic acids from the microorganisms possibly present on said filtration membrane. | 09-23-2010 |
20100240024 | Assays And Kits For Determining HIV-1 Tropism - The present invention relates to assays and kits for determining tropism of HIV-1 for a chemokine receptor in a test sample obtained from a subject. | 09-23-2010 |
20100240025 | Bacteriophage with Enhanced Lytic Activity - This invention encompasses an isolated | 09-23-2010 |
20100240026 | KIT AND METHOD FOR DETECTING BOVINE VIRAL DIARRHEA VIRUS IN TISSUE SAMPLES - The present invention relates to the method for treatment of tissue samples with proteolytic/histolytic additive collagenase or other similar protease prior to testing with an antigen capture immunoassay to identify cattle infected with Bovine Viral Diarrhea Virus (BVDV). The use of collagenase or other similar protease in antigen extraction step of the assay drastically increases accuracy of the assay, thus it allows for a more effective, reliable, quick, and cost effective way of identifying and thereby removing infected cattle and/or other animals from an otherwise uninfected herd. | 09-23-2010 |
20100240027 | Surrogate Markers for Viral Infections and Other Inflammatory Responses - Compositions and methods for the detection, diagnosis and treatment of BVDV and other viruses are provided. | 09-23-2010 |
20100248209 | Three-dimensional integrated circuit for analyte detection - The embodiments of the invention relate to a device having a first substrate comprising a transistor; a second substrate; an insulating layer in between and adjoining the first and second substrates; and an opening within the second substrate, the opening being aligned with the transistor; wherein the transistor is configured to detect an electrical charge change within the opening. Other embodiments relate to a method including providing a substrate comprising a first part, a second part, and an insulating layer in between and adjoining the first and second parts; fabricating a transistor on the first part; and fabricating an opening within the second part, the opening being aligned with the transistor; wherein the transistor is configured to detect an electrical charge change within the opening. | 09-30-2010 |
20100248210 | Nucleic acid primer set for detection of drug-resistant strain of hepatitis B virus, assay kit, and method of detecting drug-resistant strain of hepatitis B virus - The invention provides a method of detecting a drug-resistant strain of hepatitis B virus, including amplifying a hepatitis B virus nucleic acid in a sample solution by LAMP with a primer set to yield an amplification product, and hybridizing the amplification product with a probe containing a polynucleotide derived from a drug-resistant strain and/or a probe containing a polynucleotide derived from a drug-nonresistant strain, to detect a drug-resistant strain of hepatitis B virus. | 09-30-2010 |
20100248211 | METHOD FOR ANALYSIS OF HEPATITIS B VIRUS S ANTIGEN - An object of the present invention is to enable to accurately quantify HBs antigen in the samples for which measured values are low or false-negative results by the conventional assaying method of HBs antigen. In the method of assaying HBs antigen according to the present invention, at least one inner capture probe that binds to a first inner region peptide consisting of 26th to 80th amino acid residues of HBs antigen and at least one outer capture probe that binds to a second outer region peptide consisting of 98th to 156th amino acid residues of HBs antigen are used as capture probes; and at least one inner detection probe which binds to the first inner region and at least one outer detection probe which binds to the second outer region are used as detection probes. | 09-30-2010 |
20100248212 | DETECTION CONJUGATE - The invention relates to a detection conjugate composed of a filament fragment, e.g. a cytoskeletal filament such as actin filaments or microtubules, and recognition elements bound to this fragment as well as kits comprising said detection conjugate and methods how to use said detection conjugate as well as the use for the detection of one or more compounds present within a sample, such as a biological sample. | 09-30-2010 |
20100248213 | COLLECTION/EXTRACTION CONTAINER FOR BIOLOGICAL MATERIAL IN FORENSIC SAMPLES - Relates to a collection/extraction container ( | 09-30-2010 |
20100248214 | MICROORGANISM CONCENTRATION PROCESS - A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a particulate concentration agent that comprises gamma-FeO(OH); (b) providing a fluid sample comprising at least one microorganism strain; and (c) contacting the concentration agent with the sample such that at least a portion of the concentration agent is dispersed in the sample and at least a portion of the at least one microorganism strain is bound to or captured by the concentration agent. | 09-30-2010 |
20100248215 | SAMPLE PREPARATION CONTAINER AND METHOD - A system and method for preparing and delivering samples for analyte testing. The system can include a sample preparation system and a sample delivery system coupled to the sample preparation system. The sample preparation system can include a deformable self-supporting receptacle comprising a reservoir adapted to contain a liquid composition comprising a source and a diluent. The sample delivery system can include a valve positioned in fluid communication with the reservoir and adapted to control the removal of a sample from the sample preparation system. The method can include applying pressure to the deformable self-supporting receptacle to remove a sample from the sample preparation system via the sample delivery system. | 09-30-2010 |
20100248216 | SAMPLE PREPARATION CONTAINER AND METHOD - A system and method for preparing and analyzing samples. The system can include a sample preparation system and a sample detection system coupled to the sample preparation system. The sample preparation system can include a deformable self-supporting receptacle comprising a reservoir adapted to contain a liquid composition comprising a source and a diluent. The sample detection system can be positioned in fluid communication with the reservoir, and can be adapted to analyze a sample of the liquid composition for an analyte of interest. The system can further include a fluid path defined at least partially by the reservoir and the sample detection system. The method can include applying pressure to the deformable self-supporting receptacle to move a sample of the liquid composition in the fluid path to the sample detection system, and analyzing the sample for the analyte of interest with the sample detection system. | 09-30-2010 |
20100248217 | ROUNDWORM COPROANTIGEN DETECTION - A composition, device, kit and method for detecting the presence or absence of roundworm in a fecal sample. The composition, device, kit and method of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm. | 09-30-2010 |
20100255459 | CONTROL FOR VIRUS DETECTION ASSAYS BASED ON REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION - A method for the accurate quantification of a virus in a sample, by reverse transcription polymerase chain reaction (RT-PCR), includes: adding a known concentration of a Mengo virus to the sample as control for the nucleic acids extraction step, the Mengo virus being a mutant strain with the same growth properties than those of the wild-type Mengo virus, and with non-pathogenic capacity; performing a nucleic acids extraction to obtain a nucleic acids suspension; analyzing the nucleic acids suspension by RT-PCR with primers and probes; quantifying the amplimers resulting from the RT-PCR; determining the concentration of the virus in the sample by comparison of the value obtained with an appropriate standard curve; and determining the concentration of the Mengo virus by comparison of the value obtained with an appropriate standard curve. | 10-07-2010 |
20100255460 | DEVICE FOR BIOCHEMICAL PROCESSING AND ANALYSIS OF A SAMPLE - A device for biochemical processing and analysis of a measured sample volume of a sample is described. The device is characterised in that it consists of a sealed vessel ( | 10-07-2010 |
20100255461 | METHOD FOR PRODUCTION OF MOLDED BODIES, IN PARTICULAR OPTICAL STRUCTURES AND USE THEREOF - The present invention relates to a method for manufacture a body from a thermoplastic plastic with a three-dimensionally structured surface, wherein molding is performed directly from a master made of glass coated with metal oxide, without deposition of further coatings on a surface of the master. The invention also relates to bodies manufactured with this method from a thermoplastic featuring a three-dimensionally structured surface, as well as to planar optical structures likewise manufactured with this method for generating evanescent-field measuring platforms and to a use thereof. The invention furthermore relates to a planar optical structure for generating an evanescent-field measuring platform, comprising a first essentially optical transparent, waveguiding layer (a) with refractive index n | 10-07-2010 |
20100261153 | Methods For Direct Fluorescent Antibody Virus Detection In Liquids - The present invention describes a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The assay includes centrifugation of the specimen, incubation of sample and reagents in solution, and detection of the absence or presence of respiratory virus. Sapogenin is used as a detergent to permeabilize the cells for entry of the monoclonal antibodies to react with intracellular antigens. The cells are stained with fluorescently labeled monoclonal antibodies against the viral antigens along with a background stain and a fluorescent nuclear stain. This counter staining decreases background and allows co-localization of antigen and nuclear structures for enhanced detection. | 10-14-2010 |
20100261154 | PRIMERS AND PROBES FOR DETECTING HEPATITIS C VIRUS - The present invention relates to primers, probes, primer sets, primer and probe sets, methods and kits for detecting Hepatitis C virus in a test sample. | 10-14-2010 |
20100261155 | METHODS AND COMPOSITIONS RELATING TO VIRAL FUSION PROTEINS - Provided herein are isolated paramyxovirus pre-triggered fusion proteins, or functional fragments thereof, which contain one or more CRAC domains in a location that is away from the transmembrane domain. Also provided herein is a computer model of the structure of the pre-triggered F protein. Compositions that directly or indirectly bind and interfere with the normal activity or binding of the pre-triggered F proteins, or the CRAC domains, are useful as antiviral agents in the treatment of paramyxovirus infections. Thus, disclosed herein are methods of screening for antiviral agents, using the isolated pre-triggered F protein, or functional fragments thereof. | 10-14-2010 |
20100261156 | REAGENT FOR DETECTING HIV-1 ANTIGEN AND DETECTION METHOD - The present invention provides a reagent for detecting a HIV antigen comprising a first antibody which is a first human monoclonal antibody recognizing HIV-1 p24 antigen and being labeled with a labeling substance, a solid phase and a second antibody which is a second human monoclonal antibody recognizing HIV-1 p24 antigen and to which a substance being capable of binding to the solid phase is bound. | 10-14-2010 |
20100261157 | Device and Method for Processing a Sample Contained in a Swab for Diagnostic Analysis - A device for processing a sample contained in a swab for diagnostic analysis, includes a chamber having a first chamber portion and a second chamber portion to receive the swab and a processing fluid, wherein at least one of the first and second chamber portions is flexible. A divider may be positioned in the chamber to facilitate transferring the sample to the second chamber portion, and a delivery channel is disposed in fluid communication with at least one of the first chamber portion and the second chamber portion to deliver a processed sample for diagnostic analysis. | 10-14-2010 |
20100267005 | Crosslinking Within Coordination Complexes - Crosslinked proteins, proteins and polymers, and polymers and methods of making the same are disclosed. In one illustrative embodiment, a method is provided comprising the steps of attaching a chelator to one or more polymers; creating a coordination complex between the first protein, the second protein, and a metal ion; and crosslinking the first and second proteins by exposing the coordination complex to an oxidant. | 10-21-2010 |
20100267006 | METHOD FOR DETECTION OF CIRULENT STRAIN OF INFLUENZA TYPE-A VIRUS - The present invention provides a method for detecting a virulent strain of influenza virus in a specific, rapid and simple manner, and an assay device therefor. A method for detecting a virulent strain of influenza A virus is provided, which comprises providing a first antibody that is reactive with all influenza A virus subtypes and a second antibody that is not reactive with a virulent strain of influenza A virus but is reactive with all influenza A virus subtypes other than the virulent strain, and conducting an immunoassay to detect an antigen that shows a positive response in a reaction with the first antibody and shows a negative response in a reaction with the second antibody. | 10-21-2010 |
20100267007 | SOLID-FLUID COMPOSITION AND USES THEREOF - A nanostructure comprising a core material of a nanometric size surrounded by an envelope of ordered fluid molecules is disclosed. The core material and the envelope of ordered fluid molecules are in a steady physical state. Also disclosed, a liquid composition comprising liquid and the nanostructure. | 10-21-2010 |
20100267008 | CHIRAL INDOLE INTERMEDIATES AND THEIR FLUORESCENT CYANINE DYES CONTAINING FUNCTIONAL GROUPS - This invention relates to the functionalized cyanine dyes and more particularly, to the synthesis of chiral 3-substituted 2,3′-dimethyl-3H-indole and its derivatives as intermediates for preparation of cyanine dyes, to methods of preparing these dyes and the dyes so prepared. | 10-21-2010 |
20100267009 | METHOD FOR THE IN VITRO DIAGNOSIS AND/OR IN VITRO THERAPY MONITORING OF INFECTIONS - A method for in-vitro diagnosis and/or in-vitro therapy monitoring of infections and/or infectious diseases and differentiation between acute infections and latent or overcome infections comprising incubating eukaryotic cells with an antigen; and testing for cells (ASCs) secreting antigen-specific antibodies, the secreted antibodies of which are directed specifically against the antigen. | 10-21-2010 |
20100267010 | Method and Apparatus for Real-Time Analysis of Chemical, Biological and Explosive Substances in the Air - A device for real-time analysis of airborne chemical, biological and explosive substances has at least a gas analysis sensor, fluorescence/luminescence sensor and a sensor for determining the particle size and number of particles. Each of the sensors is connected to a multireflection cell (multipass laser cell) as an open measurement path. In addition, the device also includes an evaluation unit for the real-time analysis of chemical, biological and explosive substances. | 10-21-2010 |
20100273143 | DISCRIMINATORY POSITIVE/EXTRACTION CONTROL DNA - The present teachings generally relate to methods and kits incorporating a discriminating positive control for determining whether a particular microorganism or group of microorganisms are present in a sample, for example but not limited to a food, environmental, agricultural, biopharmaceutical, pharmaceutical, or water sample. According to certain methods, at least part of a starting material, for example but not limited to, a food, environmental, agricultural, biopharmaceutical, pharmaceutical, or water sample can be combined with a culture medium and incubated under conditions suitable for microbial growth followed by extracting microorganism and added control nucleic acids for analysis. The extracted nucleic acids are amplified and the amplified nucleic acids are detected, directly or indirectly, and the fidelity of the methods and the presence or absence of the corresponding microorganism is determined because the discriminating positive control provides both confirmatory results for the methods used but eliminates false positive results. | 10-28-2010 |
20100273144 | NOVEL USE OF GRP 94 IN VIRUS INFECTION - A novel use of GRP 94 in treatment of virus infection is provided. More specifically, a method of inhibiting virus infection by inhibiting expression of GRP 94 and/or inactivating activity of GRP 94, and a method of developing drugs for preventing and/or treating virus infection and/or diseases caused by virus infection by using GRP 94 as a target are provided. | 10-28-2010 |
20100279271 | Sample collection apparatus - A sample collection apparatus which measures biological samples and can be used to provide the early detection of respiratory diseases, for example tuberculosis induced by the pathogen | 11-04-2010 |
20100279272 | MULTIPLEXED ANALYSIS METHODS USING SERS-ACTIVE NANOPARTICLES - Methods are described for performing a multiplexed analysis of a level of target analyte in a sample, employing an identifier and a labeling reagent. Either or both of the identifier and the labeling reagent comprises a SERS-active nanoparticle associated with a SERS-active reporter with a uniquely identifiable spectroscopic signature. Interrogation of the identifier and the labeling reagent is conducted by serial coincident detection. Such methods can provide enhanced multiplexed analysis of analytes in a sample, especially with regards to improving the type of identifying reagents that are employed. | 11-04-2010 |
20100279273 | NUCLEIC ACID SEQUENCES FOR THE AMPLIFICATION AND DETECTION OF RESPIRATORY VIRUSES - The present invention relates to methods of detection, as well as assays, reagents and kits for the specific detection of 15 clinically important respiratory viruses including influenza A and B viruses, human respiratory syncytial viruses, human metapneumoviruses, human enteroviruses, all serotypes of rhinoviruses, 7 serotypes of adenoviruses, parainfluenza viruses types 1, 2, 3, and 4, as well as coronaviruses NL, 229E, OC43, and SARS-CoV. The present invention allows for the detection of each of these respiratory viruses in a single assay. | 11-04-2010 |
20100279274 | Method of Pooling and/or Concentrating Biological Specimens for Analysis - The present invention provides methods for concentrating and pooling liquid suspensions of biological specimens containing analytes of interest in a dry state. The dried biological specimens containing analytes of interest are reconstituted and released from the matrix for subsequent analysis in concentrated form. | 11-04-2010 |
20100279275 | HUMAN GASTROINTESTINAL STEM CELL-DERIVED PRIMARY INTESTINAL EPITHELIAL CELL SYSTEM AND METHODS OF USE THEREOF - The present invention relates to an intestinal primary epithelial cell system for detecting gastrointestinal segment-specific activation or suppression of a Toll-like receptor (TLR) by a target agent. The cell system includes an isolated human intestinal primary epithelial cell (HIPEC) line that expresses at least one TLR, where the HIPEC line is derived from a differentiable adult human gastrointestinal stem cell (ahGISC) line. Also disclosed are various methods of using the cell system, a kit that includes the cell system, and an isolated cell culture including an isolated HIPEC line derived from a differentiable ahGISC line. | 11-04-2010 |
20100279276 | METHODS FOR DETERMINING THE PRESENCE OF SARS CORONAVIRUS IN A SAMPLE - Methods for determining the presence of SARS-CoV in a test sample that include targeting the SARS-CoV 5′ leader sequence or the SARS-CoV 3′ terminal sequence. | 11-04-2010 |
20100279277 | METHODS OF EVALUATING A TEST AGENT IN A DISEASED CELL MODEL - The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the tissues of the immune system in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates and other materials in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interactions with the immune system, coupled with disease models to provide a more complete representation of an immune response. | 11-04-2010 |
20100285443 | Diagnostic Methods for Diseases Caused by a HPV Infection Comprising Determining the Methylation Status of the HPV Genome - Methods and kits for diagnosing and/or monitoring the progression of or otherwise staging a disease caused by a human papillomavirus (HPV) infection in a test sample obtained from a subject comprise determining the methylation status of a HPV genome. The presence of hypermethylation of the HPV genome indicates a positive diagnosis of the disease and/or an increased level of methylation of the HPV genome indicates the progression of the disease to a more advanced form. Suitable diseases linked to HPV infection include cancers such as cervical cancer. High risk HPV types such as HPV16 are generally assessed in the methods and using the kits of the invention. The HPV16 methylome is provided. | 11-11-2010 |
20100285444 | HBV precore protein capable of forming particles - There is provided a HBV precore protein having an ability of forming particles, and a means for determining it. A novel HBV precore protein that forms the virus (like) particles of HBV was identified. The present invention provides this novel HBV precore protein. Furthermore, there are provided core-like particles and virus-like particles formed by this HBV precore protein. These virus-like particles can be used for vaccines and therapeutic agents. The present invention also provides a method of determining the HBV precore protein and a method of determining the anti-HBV precore protein antibody. | 11-11-2010 |
20100285445 | CHIMERIC CONSTRUCT OF MUNGBEAN YELLOW MOSAIC INDIA VIRUS (MYMIV) AND ITS USES THEREOF - A recombinant DNA construct, recombinant vectors and host cells comprising the dimers of DNA A and DNA B of Mungbean Yellow Mosaic India Virus (MYMIV) in a single Ti plasmid are provided herein. | 11-11-2010 |
20100285446 | Methods for Detecting Metabolic States by Laser Ablation Electrospray Ionization Mass Spectrometry - According to certain embodiments, a method of mass spectrometry may generally comprise subjecting a sample comprising at least one indicator to laser ablation electrospray ionization mass spectrometry; determining a relative intensity of the indicator; and comparing the relative intensity of the indicator to a standard indicator intensity. Subjecting a sample to laser ablation electrospray ionization mass spectrometry may comprise ablating the sample with an infrared laser under ambient conditions to form an ablation plume; intercepting the ablation plume by an electrospray plume; and detecting the indicator by mass spectrometry. The method of mass spectrometry may comprise classifying the sample as belonging to or not belonging to the standard indicator intensity. A sample not belonging to the standard indicator intensity may indicate that the sample is predicted to comprise a disease state. | 11-11-2010 |
20100285447 | Methods for Antimicrobial Resistance Determination - The present invention relates to methods and systems for determining the antibiotic-resistance status of microorganisms. The invention further provides methods for determining the antibiotic-resistance status of microorganisms in situ within a single system. | 11-11-2010 |
20100285448 | RETROVIRAL NUCLEIC MATERIAL AND NUCLEOTIDE FRAGMENTS, IN PARTICULAR ASSOCIATED WITH MULTIPLE SCLEROSIS AND/OR RHEUMATOID ARTHRITIS, FOR DIAGNOSTIC, PROPHYLACTIC AND THERAPEUTIC USES - An isolated polynucleotide having a nucleotide sequence selected from the group consisting of (a) SEQ ID NO: 21, (b) the full-length sequences encoding a polypeptide having a peptide sequence selected from the group consisting of SEQ ID NOs: 25 and 26, and (c) the full-length complementary sequences to the sequences set forth in (a) or (b). | 11-11-2010 |
20100285449 | Human G-Protein Chemokine Receptor (CCR5) HDGNR10 - Human G-protein chemokine receptor polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptides for identifying antagonists and agonists to such polypeptides and methods of using the agonists and antagonists therapeutically to treat conditions related to the underexpression and overexpression of the G-protein chemokine receptor polypeptides, respectively. Also disclosed are diagnostic methods for detecting a mutation in the G-protein chemokine receptor nucleic acid sequences and detecting a level of the soluble form of the receptors in a sample derived from a host. | 11-11-2010 |
20100285450 | COMPOSITIONS AND ASSAYS TO DETECT INFLUENZA VIRUS A AND B NUCLEIC ACIDS - Methods for detecting influenza virus A and influenza virus B nucleic acids in biological samples by using in vitro amplification and detection are disclosed. Compositions that are target-specific nucleic acid sequences and kits comprising target-specific nucleic acid oligomers for amplifying in vitro influenza virus A or influenza virus B nucleic acid and detecting amplified nucleic acid sequences are disclosed. | 11-11-2010 |
20100291537 | METHODS AND COMPOSITIONS RELATED TO PHAGE-NANOPARTICLE ASSEMBLIES - Embodiments of the invention include additional compositions and related methods and devices for the use of phage-nanoparticle assemblies. Embodiments of the invention include compositions, methods and devices related to phage-nanoparticle assemblies and their use in a variety of methods including detection methods, in vitro and in vivo diagnostic methods, direct and/or indirect therapeutic methods, or combinations thereof. Phage-nanoparticle assemblies of the invention comprise a plurality of nanoparticles complexed with one or more phage particles to form a phage-nanoparticle assembly. In certain aspects, the phage-nanoparticle assembly may also include other agents, including but not limited to organizing agents and/or therapeutic agents. | 11-18-2010 |
20100291538 | ARTIFICIAL CALIBRATION VIRUS TO CONTROL HIV VIRAL LOAD TESTS BY PCR IN REAL TIME - The present invention refers to the design of an artificial calibrating virus (ACV), as well as a methodology quality guarantee system, which has controlling characteristics in the performance of all the stages carried out during a detection and/or quantification molecular test. More specifically, the referred to ACV is used for the validation and calibration of quantitative determinations of circulating viruses in blood plasma samples by means of polymerase chain reaction (PCR) technology in real time (or ‘real time PCR’). | 11-18-2010 |
20100291539 | METHODOLOGY FOR DETECTION, ENUMERATION, PROPAGATION AND MANIPULATION OF BACTERIOPHAGES - A method to propagate, enumerate and quantify bacteriophage(s) in a water sample or other aqueous sample was designed which contains ingredients to stimulate the growth of select bacterial species which are susceptible to infection by specific bacteriophage(s), in which interfering background organisms are either inhibited or inconsequential. Important features of the medium include oxidation-reduction compounds producing colored and/or fluorescent products, chromogenic and/or fluorogenic enzyme substrates, and temperature-independent gelling agent(s). A preferred combination is the growth medium containing 2,3,5-triphenyl tetrazolium chloride, 5-bromo-4-chloro-3-indolyl-B-D-galactoside, and appropriate gelling agents, which (when properly used) produces a dark red bacterial lawn containing teal blue-green, irregularly circular spots representing individual phage plaque, all discernible to the eye in visible light. The procedure can also be readily applied towards automatic counting systems under artificial illumination. The procedure can be employed with water samples and with elution buffers that can retain bacteriophages in suspension following contact by the buffer with foods, soils, hard surfaces and other solids that may be contaminated by bacteriophages. | 11-18-2010 |
20100291540 | CARBOHYDRATE BINDING MODULE AND USE THEREOF - The present invention relates to an antibody mimetic of carbohydrate binding module (CBM) which specifically binds to an epitope on HIV glycoprotein. The present invention also relates to a method of detecting HIV glycoprotein. | 11-18-2010 |
20100291541 | BACTERIOPHAGE IMMOBILIZATION FOR BIOSENSORS - A method is disclosed for anchoring a bacteriophage on a substrate, the bacteriophage having a phage amine moiety, the method comprising: producing a free amine terminal moiety on the substrate by chemical modification of the substrate; activating the free amine terminal moiety with a cross-linking agent to produce an active functional group to couple to the phage amine moiety; and anchoring the bacteriophage to the substrate using the active functional group. A sensor is also disclosed comprising: a substrate; an anchor group attached by chemical modification to the substrate and having an active functional group produced by the activation of a free amine terminal moiety; and a bacteriophage having a phage amine moiety coupled to the active functional group to anchor the bacteriophage to the substrate. | 11-18-2010 |
20100291542 | RAPID IMMUNOCHROMATOGRAPHIC DETECTION BY AMPLIFICATION OF THE COLLOIDAL GOLD SIGNAL - The present invention relates to a method for rapid immunochromatographic detection of a target in a sample by double sandwich immunoassay detection, wherein the target is an antibody and/or an antigen, using different colloidal gold conjugates conjugated with a first and a second specific antibody or antigen, respectively, to a rapid immunochromatographic detection device, to uses of the method for detecting diseases or specific conditions, and to a method for the manufacture of the device as well as to a kit which comprises the device. | 11-18-2010 |
20100291543 | HOMOGENEOUS IN VITRO FEC ASSAYS AND COMPONENTS - Reporter fragments, reporter components, and systems adapted to detect analytes in homogeneous in vitro assays are provided, such assays employing these systems, and methods of making and using same. Particular embodiments include isolated and purified reporter fragments displaying enhanced solubility, reduced aggregation, resistance to inhibitors, and enhanced suitability for use in homogeneous in vitro assays. | 11-18-2010 |
20100291544 | COMPOSITIONS FOR USE IN IDENTIFICATION OF STRAINS OF HEPATITIS C VIRUS - The present invention provides compositions, kits and methods for rapid identification and quantification of strains of hepatitis C viruses by molecular mass and base composition analysis. | 11-18-2010 |
20100291545 | ANTIBODY HAVING INHIBITORY ACTIVITY ON INFECTION WITH HEPATITIS C VIRUS (HCV) AND USE THEREOF - The objection of the invention is to provide an antibody that inhibits infection with hepatitis C virus (HCV). To this end, this invention provides an antibody that recognizes the hepatitis C virus (HCV) particle obtained from the hepatitis C virus (HCV) genome comprising the following (i) and (ii) ligated to each other as an antigen and has an inhibitory activity on infection with hepatitis C virus (HCV): (i) (a) the 5′-untranslated region, the core protein-encoding sequence, the E1 protein-encoding sequence, the E2 protein-encoding sequence, and the p7 protein-encoding sequence of the JFH-1 strain of the hepatitis C virus (HCV) or (b) the 5′-untranslated region, the core protein-encoding sequence, the E1 protein-encoding sequence, the E2 protein-encoding sequence, and the p7 protein-encoding sequence of the J6CF strain the hepatitis C virus (HCV); and (ii) the NS2 protein-encoding sequence, the NS3 protein-encoding sequence, the NS4A protein-encoding sequence, the NS4B protein-encoding sequence, the NS5A protein-encoding sequence, the NS5B protein-encoding sequence, and the 3′-untranslated region of the JFH-1 strain. | 11-18-2010 |
20100291546 | METHOD FOR DETECTION OF HEPATITIS B VIRUS - To provide a method for detection or quantification of hepatitis B virus (HBV) antigens in serum and a simple and highly user-friendly method for sample treatment for use in the detection or quantification thereof. The method for treatment of a sample containing hepatitis B virus (HBV) is characterized in that release of HBV antigens and disruption of antibodies that bind to HBV antigens are carried out by treating a sample containing HBV with a treatment agent containing (1) an acidifying agent and (2) a protein denaturant or an amphoteric surfactant or cationic surfactant having an alkyl group and a tertiary amine or a quaternary ammonium salt within a molecule. | 11-18-2010 |
20100291547 | FLUOROGENIC HYDRAZINE-SUBSTITUTED COMPOUNDS - The present disclosure is directed to fluorogenic schiff base-forming dyes capable of detecting analytes containing aldehyde and ketone groups. The dyes contain nucleophilic hydrazinyl appendages and are capable of binding and detecting analytes in situ. | 11-18-2010 |
20100297602 | Apparatus and method of contaminant detection - The present invention is a method and apparatus for contaminant detection of body parts, such as hands, and or their coverings, such as clean room suits or gloves, and small objects. Particularly, the method and apparatus involve collecting air samples containing aerosolized contaminate particles from the objects and analyzing the sample for presence of a contaminate. Aerosol lab-on-a-chip and/or electronic nose devices are utilized for the detection of contaminant particles. | 11-25-2010 |
20100297603 | ANALYTE-RELEASING BEADS AND USE THEREOF IN QUANTITATIVE ELISPOT OR FLUORISPOT ASSAY - The present invention relates to a method of quantifying analyte secreted by a cell or released from a drug delivery vehicle, typically by ELISpot or fluorispot assay. Quantification is possible through the use of an analyte-releasing reagent that includes a bead and the analyte releasably bound to the bead, or a container pre-spotted with analyte released from the reagent. The reagent or pre-spotted containers can be used to provide a standard curve for release of the analyte. By detecting analyte secreted by one or more cells or drug released by a drug delivery vehicle, and comparing the detected analyte to the standard curve, it is possible to quantify the amount of analyte released by the one or more cells or drug released by the drug delivery vehicle. Kits and reagents for practicing the methods of the present invention are also disclosed. | 11-25-2010 |
20100297604 | METHODS AND REAGENTS FOR VIRUS ISOLATION AND DETECTION - The present invention relates to reagents and methods used in virus isolation and analysis. | 11-25-2010 |
20100297605 | Screening method for prophylactic and/or therapeutic agent for disease accompanied by hepatitis c - Provided is a screening method being able to screen an agent useful for prevention and/or treatment of hepatitis C virus-related disease easily and efficiently and a prophylactic and/or therapeutic agent for hepatitis C virus-related disease obtained by the method. | 11-25-2010 |
20100297606 | IgG BINDING PEPTIDE - The present invention provides a peptide capable of specifically binding to human IgG. In particular, the present invention relates to a human IgG binding peptide tag of 11 to 16 amino acids in length, comprising at least an amino acid sequence of the formula I: | 11-25-2010 |
20100297607 | Reagents For HCV Antigen-Antibody Combination Assays - The present invention is directed to combination immunoassays, reagents and kits for simultaneous detection of HCV antigens and anti-HCV antibodies in a sample. The combination immunoassays of the present invention employ a non-ionic detergent that effectively exposes or releases the HCV core antigen from virions in a sample without interfering with the performance of other reagents such as the capture of anti-HCV antibodies by recombinant HCV antigens. | 11-25-2010 |
20100297608 | Systems and Methods for CMOS-Compatible Silicon Nano-Wire Sensors with Biochemical and Cellular Interfaces - The systems and methods described herein include a sensor for suitable for sensing chemical and biological substances. The sensor comprises a semiconductor layer formed in or on a substrate and a channel having nano-scale dimensions formed in the semiconductor layer, where the structure creates an electrically conducting pathway between a first contact and a second contact on the semiconductor layer. In certain preferred embodiments, the nano-scale channel has a trapezoidal cross-section with an effective width and exposed lateral faces, where the effective width is selected to have same order of magnitude as a Debye length (L | 11-25-2010 |
20100297609 | VIVO ISOTOPIC LABELING METHOD FOR QUANTITATIVE GLYCOMICS - The present invention relates to a method of isotopically labeling glycans and in facilitating high throughput quantitative/comparative analysis of glycomic compositions of biological cells. The method is applicable inter alia for identifying differentiated cells and their glycomic characteristics, differentiation conditions, disease and/or therapeutic progression, diagnosing disease states, determining drug activity, establishing manufacturing efficiencies and for determining the half-life of glycans in cells. | 11-25-2010 |
20100297610 | MOLECULARLY IMPRINTED POLYMERS FOR DETECTING HIV-1 - The invention described herein provides molecularly imprinted polymers (MIPs) that are capable of binding to virus, and methods for detecting and/or identifying specific virus particles utilizing Molecularly Imprinted Polymers (MIPs). The virus particles of the invention include HIV-1, HIV-2, HTLV-1, HTLV-2, HPV, HBV, and HCV. The methods of the invention comprise detecting all or part, including epitopes, of macromolecules associated with a virus. The macromolecules of the invention include proteins, glycoproteins (e.g., envelope glycoproteins), peptides, and polypeptides associated with said virus. The invention also provides for methods of diagnosing a subject infected with a virus utilizing MIPs, in addition to diagnostic kits. | 11-25-2010 |
20100297611 | Method and Device For Combined Detection Of Viral And Bacterial Infections - A lateral flow assay detects and differentiates between viral and bacterial infections. A combined point of care diagnostic device tests markers for viral infection and markers for bacterial infection, to effectively assist in the rapid differentiation of viral and bacterial infections. In one preferred embodiment, the bacterial marker is CRP. In another preferred embodiment, the viral marker is MxA. In some embodiments, it is unnecessary to lyse the cells in the sample prior to applying it to the device. | 11-25-2010 |
20100297612 | OPTIMIZED PROBES AND PRIMERS AND METHODS OF USING SAME FOR THE DETECTION, SCREENING, QUANTITATION, ISOLATION AND SEQUENCING OF CYTOMEGALOVIRUS AND EPSTEIN-BARR VIRUS - Described herein are primers and probes useful for detecting, screening, quantitating, isolating and sequencing CMV and EBV viral strains, and methods of using the described primers and probes. | 11-25-2010 |
20100304358 | METHODS OF IDENTIFYING BIOLOGICAL TARGETS AND INSTRUMENTATION TO IDENTIFY BIOLOGICAL TARGETS - Methods of measuring and/or detecting biological targets, methods of distinguishing among the same type of biological target, single-molecule detection systems, fluorescent/biological target complexes, methods of using fluorescent/biological target complexes, and the like are disclosed. | 12-02-2010 |
20100304359 | MULTIANALYTE ASSAY - The invention provides compositions, systems and methods for detecting multiple analytes from a sample. | 12-02-2010 |
20100304360 | GOLD NANOPARTICLE HPV GENOTYPING SYSTEM AND ASSAY - The invention provides oligonucleotides, kits and methods for genotyping HPV. | 12-02-2010 |
20100304361 | METHOD AND DEVICE FOR MONITORING A THERAPEUTIC TREATMENT REGIME - A method for monitoring a therapeutic treatment regime in an individual having a disease, comprises: providing at a first location a solid substrate capable of immobilising a biomarker characteristic of the disease and a therapeutic compound in the biological sample, contacting the biological sample with the solid substrate to immobilise the biomarker and the therapeutic compound; transferring the solid substrate with the immobilised biomarker and therapeutic compound to a second location; performing an extraction step on the solid substrate to extract the biomarker and the therapeutic compound; performing a first detection assay to detect and/or quantify the biomarker, performing a second detection assay to quantify the therapeutic compound; and correlating the detection and/or quantity of the biomarker with the disease state of the individual, and comparing the quantity of the therapeutic compound with a target level for treatment of the disease, thereby to assess the efficacy of the treatment regime. | 12-02-2010 |
20100304362 | NEW DETECTION METHOD FOR CERVICAL HPVS - The invention describes a consensus PCR based method (i.e. HRE7-PCR) for the simultaneous detection of 14 Human Papilloma Virus types (i.e. HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68) that are classified as (probably) high-risk, relating to the causation of cervical cancer) and a candidate hrHPV type (i.e. HPV 67) using sets of 6 overlapping forward primers and 9 overlapping backward primers that together amplify a fragment of about (215) to (245) base pairs of the E7 open reading frame of these hrHPV types. For the detection of reaction products an EIA format can be used with the aid of a cocktail of type-specific oligoprobes as exemplified herein. Furthermore, we have developed a method for an efficient typing of these (15) HPVs that is compatible with the method for detection. This RLB typing system involves hybridisation of PCR products with immobilised type-specific oligoprobes. | 12-02-2010 |
20100304363 | PEPTIDE COMPOUNDS FOR DETECTING OR INHIBITING SARS CORONAVIRUS AND APPLICATION THEREOF - Disclosed herein are peptide compounds and the application thereof to the detection and inhibition of SARS coronavirus. Composed of dipeptides, the compounds for detecting and inhibiting SARS coronavirus can be readily synthesized and produced at low cost. In addition, they can be stored safely for a long period of time. The dipeptide compounds are useful as inhibitors of SARS coronavirus as well as acting as excellent capturing materials of SARS coronavirus. | 12-02-2010 |
20100304364 | Compositions and methods for monosynaptic transport - Disclosed herein are methods of expressing a heterologous nucleic acid sequence, such as a sequence encoding a detectable protein, in a primary neuron (or plurality of primary neurons) and other neurons that are monosynaptically connected to the primary neuron (or plurality of primary neurons). Such methods involve viruses (such as, rabies viruses) defective for transsynaptic transport (TST-defective virus) and in situ complementation of the defect in a manner that permits only monosynaptic transport of the TST-defective virus. The TST-defective virus and, therefore, any heterologous nucleic acid sequence it carries in its genome, are not transmitted to neurons that are not monosynaptically connected to the primary neuron (or plurality of primary neurons). Also disclosed are methods of targeting a TST-defective virus to a genetically defined primary neuron (or plurality of primary neurons). The disclosed technology enables far more specific labelling and/or manipulation of neural networks than has previously been possible. | 12-02-2010 |
20100311038 | NEAR FULL-GENOME ASSAY OF HCV DRUG RESISTANCE - The invention relates to assays for characterization of genotypic mutations of Hepatitis C Virus (HCV) showing a resistance to anti-HCV drugs. | 12-09-2010 |
20100311039 | NON-TARGET AMPLIFICATION METHOD FOR DETECTION OF RNA SPLICE-FORMS IN A SAMPLE - Provided are methods of isolating RNA from a biological sample, methods and means for determining the presence of particular RNA splice-form variants in a biological sample, methods and means for determining the relative ratio of RNA ratios in a biological sample, and methods and means for predicting the progression of precancerous cervical lesions. | 12-09-2010 |
20100316989 | METHOD FOR SCREENING AN INHIBITORY AGENT OF HBV PROLIFERATION BY USING THE INTERACTION BETWEEN HBV CAPSID AND SURFACE PROTEINS BASED ON CELLULAR IMAGING - The present invention relates to a method for screening an inhibitory agent of HBV proliferation by measuring the interaction (binding strength) between capsid protein and surface protein, necessary for the proliferation of HBV, by using cellular imaging, more precisely a method for measuring changes on cellular imaging caused by the interaction between a fusion protein containing PreS domain of HBV surface protein and PH (Pleckstrin homology) domain sequence and a fusion protein containing capsid protein and fluorescence protein (GFP) interacting with the said fusion protein. The method of the present invention detecting the interaction between proteins necessary for HBV proliferation at cellular level can be effectively used for the screening of a novel inhibitory agent of HBV proliferation at cellular level. | 12-16-2010 |
20100316990 | Biomarkers for HPV-Induced Cancer - Biomarkers that correlate with progression to neoplasia in human papillomavirus (HPV) induced cancer, for example cervical cancer have been identified. These biomarkers can be used to diagnosis or assist in the diagnosis of HPV-induced cancer. They can also be used to increase the positive predictive value of current screening modalities. In addition, they can provide insights into the biology of HPV-induced cancer and thus provide leads for the development of nonsurgical therapies. Exemplary biomarkers include cornulin, PA28 β, DJ-1, actin, transthyretin, HSPB1, CV intracellular channel 1, cytokeratin 8, transferrin, Hsρβ6 (HSP20), aflatoxin reductase, α2 type I collagen, creatine kinase B, cytokeratin 13 GST π, PA28 α, Manganese SOD, lamin A/C, serpin B1 (elastase inhibitor), serpin B3 (SCAA1), cytokeratin 10, cytokeratin 6A, and trp-tRNA synthetase. Preferred biomarkers for HPV-induced cancer include cornulin, DJ-1, PA28 α, and PA28 β, trp-tRNA synthetase, HSPβ6, creatine kinase B, aflatoxin reductase, GST π, transthyretin, transferrin, α2-type 1 collagen, and combinations thereof. | 12-16-2010 |
20100316991 | HUMAN PARAINFLUENZA VIRUS TYPE 3 EXPRESSING THE ENHANCED GREEN FLUORESCENT PROTEIN FOR USE IN HIGH-THROUGHPUT ANTIVIRAL ASSAYS - Disclosed herein is a recombinant human parainfluenza virus expressing the enhanced green fluorescent protein. Methods of making and methods of using a recombinant human parainfluenza virus expressing the enhanced green fluorescent protein are also disclosed. A recombinant human parainfluenza virus expressing the enhanced green fluorescent protein was rescued and evaluated for its use in antiviral assays. Without limiting the invention, in one example, there is provided a cDNA clone of SEQ ID NO: 1. | 12-16-2010 |
20100316992 | DIAGNOSTIC TEST - Disclosed are methods for conducting diagnostic tests for the detection of the inflammatory bowel diseases, such as Crohn's disease and ulcerative colitis. Also described are methods for monitoring a patient by administering tests of the present invention. Also described are methods for monitoring patient's treatment by administering tests of the present invention. Also described are methods for evaluating the effectiveness of a drug or a drug candidate by administering tests of the present invention to samples from patients, animal models, and cell cultures treated with a drug or a drug candidate. Also disclosed are methods for determining the usefulness of analytes, e.g. cytokines, for acting as diagnostic and monitoring markers for inflammatory bowel disease in the various methods of the invention. | 12-16-2010 |
20100323340 | HETERODUPLEX TRACKING ASSAY - A change in viral tropism occurs in many HIV positive individuals over time and may be indicated by a shift in coreceptor use from CCR5 to CXCR4. The shift in coreceptor use to CXCR4 has been shown to correlate with increased disease progression. In patients undergoing HAART, the predominant populations of virus may be shifted back to CCR5-mediated entry soon after the CXCR4-specific strains have emerged. The present invention relates to a diagnostic method to monitor coreceptor use in the treatment and clinical management of human immunodeficiency virus (HIV) infection. The present invention further relates to a diagnostic method applied to HIV-positive individuals undergoing HAART to monitor the suppression of CCR5- or CXCR4-specific strains. The diagnostic methods may be used to assist in selecting antiretroviral therapy and to improve predictions of disease prognosis over time. The methods of the invention include cell-based methods, including cell fusion assays, and molecular-based methods, including heteroduplex tracking assay, to both quantitatively and qualitatively analyze patient-derived HIV for coreceptor usage. | 12-23-2010 |
20100323341 | HETERODUPLEX TRACKING ASSAY - A change in viral tropism occurs in many HIV positive individuals over time and may be indicated by a shift in coreceptor use from CCR5 to CXCR4. The shift in coreceptor use to CXCR4 has been shown to correlate with increased disease progression. In patients undergoing HAART, the predominant populations of virus may be shifted back to CCR5-mediated entry soon after the CXCR4-specific strains have emerged. The present invention relates to a diagnostic method to monitor coreceptor use in the treatment and clinical management of human immunodeficiency virus (HIV) infection. The present invention further relates to a diagnostic method applied to HIV-positive individuals undergoing HAART to monitor the suppression of CCR5- or CXCR4-specific strains. The diagnostic methods may be used to assist in selecting antiretroviral therapy and to improve predictions of disease prognosis over time. The methods of the invention include cell-based methods, including cell fusion assays, and molecular-based methods, including heteroduplex tracking assay, to both quantitatively and qualitatively analyze patient-derived HIV for coreceptor usage. | 12-23-2010 |
20100323342 | MICRO-DEVICE AND METHOD FOR SELECTIVE AND NON-INVASIVE SEPARATION AND EXTRACTION OF PARTICLES IN POLYDISPERSED SUSPENSIONS, MANUFACTURING PROCESS AND APPLICATIONS THEREOF - The present invention relates to a micro-device for selective and non-invasive separation of particles in polydispersed suspensions through the strategic use of ultrasounds, laminar flow and standing wave effects in a channel produced in a chip by means of microtechnology. Said device is a resonant multi-layer system with a modified lambda quarter-type treatment channel, which enables the particles to be channeled and separated in a flow inside the substrate channel without touching the walls of the device, in order to avoid problems of adherence. Said micro-device can be used in the field of biomedicine and/or biotechnology for the separation and concentration of cells, preferably human cells, applicable to research and medical processes for diagnosis and treatment. | 12-23-2010 |
20100323343 | METHODS AND COMPOSITIONS FOR ANALYTE DETECTION - The present invention is directed to methods and apparatus for detection of one or more analytes. Analytes include agents or components of infectious agents such as pathogenic virus, as well as enzymes, proteins and biomarkers. | 12-23-2010 |
20100323344 | Detection of hepatitis C virus RNA - The methods and compositions of this invention provide a means for determining the presence of Hepatitis C virus (HCV) in a sample from an individual. This is accomplished by hybridization to tissue samples using a cRNA probe that is specific for HCV. These means allow superior detection of HCV infection by virtue of the RNA riboprobe. | 12-23-2010 |
20100323345 | METHODS AND COMPOSITIONS FOR IDENTIFYING CELLS BY COMBINATORIAL FLUORESCENCE IMAGING - A method of identifying the classification of cells in situ involves labeling the cells with a set of nucleic acid probes and performing combinational fluorescence microscopic imaging. The set of probes contains groups of probes that bind to a taxon-specific or function-specific nucleotide sequence. Each probe of a group of probes is labeled with a distinct fluorescent label, and each group corresponds to a unique combination of labels, which can be detected across the image and serves to identify cells according to a unique taxonomic or functional classification. The combinational labeling and spectral imaging approach expands the number of different classifications that can be identified simultaneously in a single image of a collection of cells. The methods and probe sets of the invention can be used to rapidly identify microbes, study their ecological relationships, screen for novel antibiotics, and identify pathogens. | 12-23-2010 |
20100330548 | Nucleic Acid Primers and Probes for Detecting Human and Avian Influenza Viruses - Provided are nucleic acid sequences that are used to prepare primers and probes that are used in a kinetic polymerase chain reaction (kPCR) assay to detect influenza viruses in a human or animal subject. The starting material for the kPCR assays may be DNA or RNA and the assays may be conducted in a singleplex assay to detect a single influenza virus or in a multiplex assay to detect multiple influenza viruses. The primers and probes have utility in the detection and quantification of type A and type B influenza viruses (INFA and INFB, respectively) and have been shown to be effective for the detection and quantification of all the known INFA subtypes, namely, H1, H2, H3, H4, H5, H6, H7, H8, and H9. | 12-30-2010 |
20100330549 | HETERODUPLEX TRACKING ASSAY - A change in viral tropism occurs in many HIV positive individuals over time and may be indicated by a shift in coreceptor use from CCR5 to CXCR4. The shift in coreceptor use to CXCR4 has been shown to correlate with increased disease progression. In patients undergoing HAART, the predominant populations of virus may be shifted back to CCR5-mediated entry soon after the CXCR4-specific strains have emerged. The present invention relates to a diagnostic method to monitor coreceptor use in the treatment and clinical management of human immunodeficiency virus (HIV) infection. The present invention further relates to a diagnostic method applied to HIV-positive individuals undergoing HAART to monitor the suppression of CCR5- or CXCR4-specific strains. The diagnostic methods may be used to assist in selecting antiretroviral therapy and to improve predictions of disease prognosis over time. The methods of the invention include cell-based methods, including cell fusion assays, and molecular-based methods, including heteroduplex tracking assay, to both quantitatively and qualitatively analyze patient-derived HIV for coreceptor usage. | 12-30-2010 |
20100330550 | DETECTION OF HUMAN PAPILLOMAVIRUS - The present invention relates to in vitro methods of screening human subjects for the presence of human papillomavirus (HPV) which exhibits loss of regulation of E6/E7 mRNA expression and loss of replication and/or expression of a stabilized pre-mRNA encoding full length E6 protein. In particular, the invention provides in vitro methods of screening for persistent transforming HPV infection equivalent to persistent cell abnormalities or persistent CIN III lesions, cancer in situ or high-grade squamous intraepithelial lesions (HSIL). The methods are useful in the context of cervical cancer screening. | 12-30-2010 |
20110003278 | H5 Subtype-Specific Binding Proteins Useful for H5 Avian Influenza Diagnosis and Surveillance - The invention provides monoclonal antibodies and related binding proteins that bind specifically to the envelope glycoprotein of H5 subtypes of avian influenza virus (“AIV”). The monoclonal antibodies and related binding proteins are useful for the detection of H5 subtypes of AIV, including the pathogenic H5N1 subtypes. Virus may be detected in formalin preserved, paraffin embeded specimens as well as frozen specimens and biological fluids. Accordingly, the invention provides means for the diagnosis and surveillance of dangerous viral infections. | 01-06-2011 |
20110003279 | Monitoring devices and processes based on transformation, destruction and conversion of nanostructures - A large number of properties of nanostructures depend on their size, shape and many other parameters. As the size of a nanostructure decreases, there is a rapid change in many properties. When the nanostructure is completely destroyed, those properties essentially disappear. Systems based on changes in properties of nanostructures due to the destruction of nanostructures are proposed. The systems can be used for monitoring the total exposure to organic, inorganic, organometallic and biological compounds and agents using analytical methods. | 01-06-2011 |
20110003280 | IMMUNOASSAY APPARATUS AND IMMUNOASSAY METHOD - The present invention relates to an immunoassay apparatus comprising a measurement unit | 01-06-2011 |
20110008765 | USE OF THREE-DIMENSIONAL MICROFABRICATED TISSUE ENGINEERED SYSTEMS FOR PHARMACOLOGIC APPLICATIONS - The present invention generally relates to a combination of the fields of tissue engineering, drug discovery and drug development. It more specifically provides new methods and materials for testing the efficacy and safety of experimental drugs, defining the metabolic pathways of experimental drugs and characterizing the properties (e.g., side effects, new uses) of existing drugs. Preferably, evaluation is carried out in three-dimensional tissue-engineered systems, wherein drug toxicity, metabolism, interaction and/or efficacy can be determined. | 01-13-2011 |
20110008766 | Dual Variable Domain Immunoglobulins and Uses Thereof - Engineered multivalent and multispecific binding proteins, methods of making, and specifically to their uses in the prevention, diagnosis, and/or treatment of disease. | 01-13-2011 |
20110008767 | MICROFLUIDIC DEVICE - The present disclosure relates to microfluidic devices adapted for facilitating cytometry analysis of particles flowing therethrough. In certain embodiments, the microfluidic devices allow light collection from multiple directions. In certain other embodiments, the microfluidic devices use spatial intensity modulation. In certain other embodiments, the microfluidic devices have magnetic field separators. In certain other embodiments, the microfluidic devices have the ability to stack. In certain other embodiments, the microfluidic devices have 3-D hydrodynamic focusing to align sperm cells. In certain other embodiments, the microfluidic devices have acoustic energy couplers. In certain other embodiments, the microfluidic devices have phase variation producing lenses. In certain other embodiments, the microfluidic devices have transmissive and reflective lenses. In certain other embodiments, the microfluidic devices have integrally-formed optics. In certain other embodiments, the microfluidic devices have non-integral geographically selective reagent delivery structures. In certain other embodiments, the microfluidic devices have optical waveguides incorporated into their flow channels. In certain other embodiments, the microfluidic devices have optical waveguides with reflective surfaces incorporated into their flow channels. In certain other embodiments, the microfluidic devices have virus detecting and sorting capabilities. In certain other embodiments, the microfluidic devices display a color change to indicate use or a result. | 01-13-2011 |
20110008768 | OLIGONUCLEOTIDES AND METHODS FOR DETECTION OF WEST NILE VIRUS - The invention provides methods of detecting West Nile virus and oligonucleotide reagents derived from a West Nile virus consensus sequence that are useful in the methods of the invention. | 01-13-2011 |
20110014598 | OPTIMIZED PROBES AND PRIMERS AND METHOD OF USING SAME FOR THE DETECTION OF HERPES SIMPLEX VIRUS - Described herein are primers and probes useful for detecting and typing variant HSV strains, and methods of using the described primers and probes. | 01-20-2011 |
20110014599 | Novel Fluorescent Dyes and Uses Thereof - The present invention provides fluorescent dyes that are based on firefly luciferin structure. These dyes are optimally excited at shorter wavelengths and have Stokes shift of at least 50 nm. The fluorescent dyes of the invention are useful for preparation of dye-conjugates, which can be used in detection of an analyte in a sample. | 01-20-2011 |
20110014600 | Microfluidic Magnetophoretic Device and Methods for Using the Same - A microfluidic device may employ one or more sorting stations for separating target species from other species in a sample. The separation is driven by magnetophoresis. A sorting station generally includes separate buffer and sample streams. A magnetic field gradient applied to the sorting station deflects the flow path of magnetic particles (which selectively label the target species) from a sample stream into a buffer stream. The buffer stream leaving the sorting station is used to detect or further process purified target species labeled with the magnetic particles. | 01-20-2011 |
20110020785 | Diagnostic Information Generation and Use - This invention relates generally to a process for producing machine readable contextual diagnostic information and use of contextual diagnostic information for generating tangible and useful results. The process provides the highest level of integration of diagnostic information collected in a distributed or centralized system comprising diagnostic devices and a global computer network. More particularly, in certain embodiments, contextual diagnostic information are used in specific diagnostic related applications and business models including ecommerce. | 01-27-2011 |
20110020786 | PEPTIDE DENDRIMERS: AFFINITY REAGENTS FOR BINDING NOROVIRUSES - Noroviruses are recognized as the most common cause of outbreaks of acute gastroenteritis in humans. Therefore, the present invention relates to peptides or dendrimers that bind Noroviruses and the methods for identifying and synthesizing these peptides. It also relates to the detection of Noroviruses using said peptides or dendrimers formed by them. | 01-27-2011 |
20110020787 | NMR DEVICE FOR DETECTION OF ANALYTES - This invention relates generally to detection devices having one or more small wells each surrounded by, or in close proximity to, an NMR micro coil, each well containing a liquid sample with magnetic nanoparticles that self-assemble or disperse in the presence of a target analyte, thereby altering the measured NMR properties of the liquid sample. The device may be used, for example, as a portable unit for point of care diagnosis and/or field use, or the device may be implanted for continuous or intermittent monitoring of one or more biological species of interest in a patient. | 01-27-2011 |
20110020788 | NMR DEVICE FOR DETECTION OF ANALYTES - This invention relates generally to detection devices having one or more small wells each surrounded by, or in close proximity to, an NMR micro coil, each well containing a liquid sample with magnetic nanoparticles that self-assemble or disperse in the presence of a target analyte, thereby altering the measured NMR properties of the liquid sample. The device may be used, for example, as a portable unit for point of care diagnosis and/or field use, or the device may be implanted for continuous or intermittent monitoring of one or more biological species of interest in a patient. | 01-27-2011 |
20110020789 | METHODS FOR ASSESSING FATIGUE LEVEL AND APPLICATIONS THEREOF - Level of fatigue that accompanies everyday life or a disease can be simply, easily, and quantitatively assessed by obtaining a body fluid from a test subject and measuring the amount of human herpesvirus in the body fluid. Furthermore, the anti-fatigue potency of anti-fatigue substances and anti-fatigue food products can be measured. | 01-27-2011 |
20110020790 | USE OF REGULATORY SEQUENCES FOR SPECIFIC, TRANSIENT EXPRESSION IN NEURONAL DETERMINED CELLS - The present invention relates to the use of regulatory sequences for mediating specific, early transient expression in proliferative neuronal determined cells. Furthermore, the uses of recombinant nucleic acid molecules comprising said defined regulatory sequences for mediating specific, early transient expression in proliferative neuronal determined cells as well as for the generation of non-human transgenic organisms and/or host cells are disclosed. In addition, the invention provides for transgenic non-human animals and/or host cells comprising said regulatory sequences and/or recombinant nucleic acid molecules. The invention also describes methods for the preparation of such vectors, host cells and transgenic non-human animals as well as methods for the detection and/or isolation of neuronal determined cells. Additionally, methods for screening of compounds capable of regulating neuronal determined cell activity, neurogenesis, stimulating proliferation of neuronally committed precursor cells and/or neuronal differentiation are provided and the invention also relates to methods for the detection and analysis of neuronal differentiation, neuronal migration and/or neuronal determination processes. Finally, the invention relates to diagnostic and pharmaceutical compositions comprising the regulatory sequences, recombinant nucleic acid molecules, host-cells or isolated neuronal determined cells described herein. | 01-27-2011 |
20110020791 | Methods and Materials for Detecting Mutations in Quasispecies Having Length Polymorphisms - The present invention is directed to a method for detecting the presence or absence of a mutation of interest in the nucleic acid of a pathogen, wherein the mutation of interest is located adjacent to a length polymorphism defining multiple quasispecies of the pathogen. | 01-27-2011 |
20110027772 | Antigen Detection Kit and Method - An antigen detection kit and an antigen detection method using the same are provided. The antigen detection kit comprises a capture antibody, a detection antibody bound to a single stranded DNA oligonucleotide, a single stranded RNA oligonucleotide complementary sequence to the DNA oligonucleotide, and an RNase. | 02-03-2011 |
20110027773 | MASS SPECTROMETRIC METHODS FOR DETECTING MUTATIONS IN A TARGET NUCLEIC ACID - Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and mutations in the molecules are provided. In some embodiments, a process comprises: amplifying a nucleic acid from a biological sample; ionizing and volatilizing the amplified product; analyzing the product by mass spectrometry to determine an observed molecular mass of the product; and comparing the observed molecular mass of the product to a calculated molecular mass of at least one nucleic acid having a known sequence, wherein the calculated molecular mass of the at least one nucleic acid having a known sequence is derived from the base composition of the at least one nucleic acid having a known sequence; whereby the presence or absence of the target nucleic acid is detected based on the comparison. | 02-03-2011 |
20110027774 | HIGHLY SENSITIVE METHOD FOR DETECTION OF VIRAL HIV DNA REMAINING AFTER ANTIRETROVIRAL THERAPY OF AIDS PATIENTS - Methods for detecting polynucleotides, especially the DNA replicated from samples obtained from subjects infected with pathogenic viruses such as human immunodefiency virus, by detecting electromagnetic signals (“EMS”) emitted by such polynucleotides, and methods for improving the sensitivity of the polymerase chain reaction (“PCR”). | 02-03-2011 |
20110027775 | DETECTION OF INFLUENZA VIRUS - A change in strain of flu and consequently pandemic potential can be determined by assessing the presence or absence of a PL motif. The 2009 swine flu illustrates the utility of such a test. The swine flu is a subtype H1N1 influenza A. Swine flu differs from the vast majority of influenza H1N1 subtype strains from 1981-2008 or H3N2 strains from 1985 to the present in that its NS1 protein lacks a PL motif. PDZ polypeptides can be used to identify such strains and distinguish them from strains in which PL motifs are present. | 02-03-2011 |
20110033836 | METHODS FOR DETERMINING THE PRESENCE OF ANTIBODIES BLOCKING VIRAL INFECTION - The present invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic acid of step (a), and (ii) a viral expression vector which lacks a nucleic acid encoding an envelope protein, and which comprises an indicator nucleic acid which produces a detectable signal, such that the first cell produces viral particles comprising the envelope protein encoded by the nucleic acid obtained from the patient; (c) contacting the viral particles produced in step (b) with a second cell in the presence of the compound, wherein the second cell expresses a cell surface receptor to which the virus binds; (d) measuring the amount of signal produced by the second cell in order to determine the infectivity of the viral particles; and (e) comparing the amount of signal measured in step (d) with the amount of signal produced in the absence of the compound, wherein a reduced amount of signal measured in the presence of the compound indicates that the compound inhibits entry of the virus into the second cell. | 02-10-2011 |
20110033837 | IMMORTALIZED HUMAN CD4-POSITIVE CELL AND ITS USE FOR DETERMINING THE PHENOTYPE OF A HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 - The invention relates to an immortalized CD4-positive cell. According to the invention, this cell comprises a gene encoding a CXCR4 chemokine receptor that is capable of serving as a coreceptor for entry of a Human Immunodeficiency Virus Type 1 (HIV-I) into the cell, and a gene encoding a CCR5 receptor that is not capable of serving as a coreceptor for entry of a Human Immunodeficiency Virus Type 1 into the cell. The invention furthermore relates to a method for the phenotypical characterization of a HIV-I, comprising providing such a cell, adding an HIV-I to the cell under conditions that allow for the entrance of the HIV-I into the cell and the replication of the virus in the cell, and measuring the replication of the HIV-I in the cell. | 02-10-2011 |
20110033838 | SURFACE ANTIGEN PROTEIN MUTANT OF HEPATITIS B VIRUS SURFACE ANTIGEN - The disclosure relates, in some embodiments, to sequences of a novel mutant or variant of the hepatitis B surface antigen (HBsAg) and methods for detecting this genome and protein variant, and antibodies directed against it, from patients' samples. | 02-10-2011 |
20110033839 | Methods of Detecting Signatures of Disease or Conditions in Bodily Fluids - Methods and compositions for diagnosing the presence of a cancer cell in an individual are provided. Methods and compositions for identifying a tumor-specific signature in an individual having cancer are also provided. Methods and compositions for diagnosing the presence of an infectious agent in an individual and/or for identifying an infectious agent-specific signature in an infected individual are provided. Methods and compositions for diagnosing the presence of a disease in an individual are also provided. Methods and compositions for identifying a disease-specific signature in an individual having the disease are also provided. | 02-10-2011 |
20110033840 | VIRAL DETECTION LIPOSOMES AND METHOD - A method of generating pathogen detecting liposomes includes a step of providing molecular beacons with fluorescing components. The molecular beacons include either strands of RNA or DNA and the fluorescing components include an emitter and a quencher. The method further uses nanodroplet technology to encapsulate the molecular beacons within a lipid membrane. Subsequently, receptors are assembled in association with the membrane. | 02-10-2011 |
20110033841 | METHODS AND KITS FOR DIAGNOSIS, PROGNOSIS OR MONITORING OF EPSTEIN-BARR VIRUS (EBV)-ASSOCIATED CANCER - Disclosed is a non-invasive method for diagnosis, prognosis or monitoring of Epstein-Barr virus (EBV)-associated cancer by detecting and/or quantifying EBV associated nucleic acid fragments in a urine sample from an individual. Kits for diagnosis, prognosis or monitoring of cancer are also disclosed. | 02-10-2011 |
20110033842 | Skin Sampling Kit Which Stores Nucleic Acids In Stable Status, Genetic Test Methods By Using The Kit And Their Practical Application - The present invention relates to a new skin gene card for genetic test, a method for acquiring DNA and RNA and performing various genetic tests using the same, and practical applications thereof. More specifically, the inventors of the present invention have developed a skin gene card capable of acquiring samples from human skin, hair or mucosa simply, safely and quickly and enabling stable long-term storage and transport of DNA and RNA included in the acquired sample at room temperature. Various genetic tests may performed using the acquired DNA and RNA, including polymerase chain reaction (PCR), reverse transcription (RT)-PCR, real-time PCR, sequencing, hybridization, DNA chip analysis, single-nucleotide polymorphism (SNP) assay, gene mutation assay, promoter methylation assay, gene expression assay, etc. The genetic skin test result may be utilized for disease prognosis, nutrigenomic test, pharmacogenomic test, forensic test such as personal identification, diagnosis of genetic diseases, diagnosis of skin diseases, or the like. In addition, through an objective evaluation of the skin or hair condition, a personalized cosmetic and skin care system may be established for practical application in beauty care, cosmetology, dermatology, and clinical practice. | 02-10-2011 |
20110039254 | Chromosomal Insertion of Gfp Into Bacteria For Quality Control - An isolated mutated green fluorescent protein (gfp) gene for chromosomal insertion into a bacterium, wherein the gene is capable of being expressed in bacteria and produce sufficient fluorescence under illumination from a UV lamp in a bacterial colony to be seen by the naked eye. A gene cassette for inserting a gene into a chromosome. | 02-17-2011 |
20110039255 | METHOD AND SYSTEM FOR DETECTION OF A SELECTED TYPE OF MOLECULES IN A SAMPLE - The present invention refers to a method for detecting molecules and/or substances within a sample based on the use of a microcantilever system. The method comprises the variation of a certain condition such as humidity so as the mechanical feature analysed varies with a characteristic pattern while the target molecule is bound to the detector. The invention also refers to the system used to carry out such method. | 02-17-2011 |
20110039256 | DETECTION METHOD - A method for detecting the presence of a diagnostic moiety indicative of exposure to an infectious organism in a biological sample taken from a human or animal, said method comprising; (a) adding to said sample a first fluorescently labelled reagent which binds said diagnostic moiety, and a second fluorescently labelled reagent which either binds said diagnostic moiety in addition to said first fluorescently labelled reagent, or which binds the first fluorescently labelled reagent or a complex comprising the first fluorescently labelled reagent in competition to the said diagnostic moiety, wherein a label on one of the first or second fluorescently labelled reagent acts as a fluorescent energy donor compound and wherein the other of the first or second fluorescently labelled reagent acts as a fluorescent energy acceptor compound which is able to accept fluorescent energy from said donor compound; (b) exciting the fluorescent energy donor compound by illuminating with light of a wavelength which is absorbed by said fluorescent energy donor compound; (c) measuring fluorescent signal emitted by said fluorescent energy acceptor compound as a result of its absorption of the fluorescent energy from the donor compound after a time delay; and (d) relating the results to the presence or absence of diagnostic moiety in said sample. | 02-17-2011 |
20110045456 | COMPOSITIONS FOR USE IN IDENTIFICATION OF ADVENTITIOUS CONTAMINANT VIRUSES - The present invention provides oligonucleotide primers, compositions, and kits containing the same for rapid identification of viruses by amplification of a segment of viral nucleic acid followed by molecular mass analysis. | 02-24-2011 |
20110045457 | ASSAYS FOR ADSORBED INFLUENZA VACCINES - Influenza hemagglutinin (HA) binds to aluminium salt adjuvants and cannot easily be directly assayed directly a single radial immunodiffusion (SRID) test. The invention modifies the SRID protocol for an adsorbed antigen by including a step in which antigen is desorbed prior to diffusion. | 02-24-2011 |
20110045458 | Detection of Enterovirus - This document describes methods and materials relating to viral diagnostics, and more particularly to the detection of enterovirus. For example, primers and probes for the detection of enterovirus are provided. Articles of manufacture containing such primers and probes for detecting enterovirus are further provided. | 02-24-2011 |
20110053138 | Universal multi-variant detection system - The present invention provides a method to diagnostically detect the variants of a given pathogen, such as HIV, hepatitis C, hepatitis B (HBV), Parvovirus B19, etc., with the use of a single detection probe. | 03-03-2011 |
20110053139 | Detection of bioagents using a shear horizontal surface acoustic wave biosensor - Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 50×10 | 03-03-2011 |
20110053140 | Methods and reagents for diagnosing hantavirus infection - Novel methods and immunodiagnostic test kits for the detection of hantavirus infection are disclosed. The methods and kits employ combinations of recombinant N and/or G1 antigens from at least six different hantavirus serotypes, including Hantann (HTNV), Puumala (PUUV), Seoul (SEOV), Dobrava (DOBV), Sin Nombre (SNV) and Andes (ANDV). Additional hantavirus antigens from these and other hantavirus types may also be present. The methods provide for highly accurate results and allow the detection of infection so that treatment can be administered and death avoided. | 03-03-2011 |
20110053141 | Methods for ultrasensitive detection and quantification of mutant hepatitis B viruses - This invention provides compositions and methods for ultrasensitive detection and quantification of mutant hepatitis B viruses (HBV). The compositions and methods of the invention can be used to detect HBV mutations for diagnostic and prognostic purposes. This invention also provides new application of a TaqMan hydrolysis probe in asymmetric real time PCR and melting curve analysis. | 03-03-2011 |
20110053142 | Binding Protein and Epitope-Blocking Elisa for the Universal Detection of H5-Subtype Influenza Viruses - Monoclonal antibodies and related binding proteins specific to influenza H5 subtype HA protein can be used in serological diagnosis of influenza H5 infection in mammalian and avian serum samples, including human serum samples. Each antibody reacts strongly with a wide variety of strains of H5 subtype and does not show cross-reactivity with non-H5 influenza subtypes. | 03-03-2011 |
20110053143 | METHODS AND KITS FOR DETERMINING VIRAL LOAD IN CLINICAL SAMPLES - Methods and kits for determining load of an infectious agent in a sample are described, comprising performing at least one hybridization assay and calculating the load of the infectious agent in the sample from a detected nucleic acid. In particular, the methods and kits disclosed determine the load of human papillomavirus (HPV) in a sample. | 03-03-2011 |
20110053144 | PROCESS AND SYSTEM FOR DETECTING AND/OR QUANTIFYING BACTERIOPHAGES CAPABLE OF INFECTING A PREDETERMINED BACTERIAL HOST STRAIN, USE OF A MICROELECTRONIC SENSOR DEVICE FOR DETECTING SAID BACTERIOPHAGES AND MICROELECTRONIC SENSOR FOR CARRYING OUT SAID PROCESS - The process, system and device being claimed are based on the measurement of the changes in impedance produced in the interface of an electrode whereonto bacteria from a host strain have been previously adhered for detecting the desired bacteriophage. The changes produced in said electrode-bacteria interface originate in the phagic action of the bacteriophages on the bacteria adhered onto the surface of the work electrode of a microelectronic sensor device. | 03-03-2011 |
20110053145 | QUANTITATION METHOD OF VIRUS - The present invention relates to a method of quantitatively determining the number of human herpesvirus (HHV) collected from a body fluid and a kit for performing the method. Conventionally, a trained technician has been required to accurately quantitatively determine a number of HHV collected from a body fluid. The method of the present invention is a novel method of quantitative determination that enables measurement of a number of HHV in a body fluid to be simply, accurately, and efficiently determined. The method of the present invention can enable continuous evaluation of the number of HHV in body fluids and, therefore, can be applied to quantitative evaluation of the accumulation of fatigue. | 03-03-2011 |
20110059430 | CROSS-SPOT - The invention relates to an in vitro method for measuring the presence of an antigen indicative for the presence of an infectious agent, and preferably a medical condition or disease in a sample using an immune effector cell capable of producing an immune effector molecule following stimulation by an antigen. | 03-10-2011 |
20110059431 | NON-ENZYMATIC DETECTION OF BACTERIAL GENOMIC DNA - The present invention relates to methods for detecting for the presence of one or more target analytes, e.g. biomolecules, in a sample. In particular, the present invention relates to a method that utilizes blocking strands to inhibit target rehybridization to detect double stranded genomic DNA. | 03-10-2011 |
20110065086 | Methods of producing homogeneous plastic-adherent aptamer-magnetic bead-fluorophore and other sandwich assays - Methods are described for assembly of DNA aptamer-magnetic bead (“MB”) conjugate plus aptamer-quantum dot (“QD”) aptamer-fluorescent nanoparticle or other aptamer-fluorophore, aptamer-chemiluminescent reporter, aptamer-radioisotope or other aptamer-reporter conjugate sandwich assays that enable adherence to glass, polystyrene and other plastics. Adherence to glass or plastics enables detection of surface-concentrated partitioning of fluorescence versus background (bulk solution) fluorescence in one step (without a wash step) even when the external magnetic field for concentrating the assay is removed. This assay format enables rapid, one-step (homogeneous) assays for a variety of analytes without wash steps that do not sacrifice sensitivity. | 03-17-2011 |
20110065087 | NOVEL HIV TARGETS - A set of cellular genes that were identified by siRNA screening as being essential for Human Immunodeficiency Virus (HIV) infection. These genes are host cellular factors involved in DNA repair, specifically in the base excision repair pathway. | 03-17-2011 |
20110065088 | METHOD AND DEVICES FOR RAPID DIAGNOSIS OF FOOT-AND-MOUTH DISEASE - A rapid immunoassay method and apparatus for detecting foot and mouth disease virus are disclosed. The method and test device permit pen-side testing of animals and provide test results within a relatively short time period. In a preferred embodiment, the method and apparatus provide a means for differentiating between FMDV-infected and FMDV-vaccinated animals. | 03-17-2011 |
20110065089 | NOVEL STRAIN OF SARS-ASSOCIATED CORONAVIRUS AND APPLICATIONS THEREOF - The invention relates to a novel strain of severe acute respiratory syndrome (SARS)-associated coronavirus, resulting from a sample collected in Hanoi (Vietnam), reference number 031589, nucleic acid molecules originating from the genome of same, proteins and peptides coded by said nucleic acid molecules and, more specifically, protein N and the applications thereof, for example, as diagnostic reagents and/or as a vaccine. | 03-17-2011 |
20110065090 | Asymmetrically branched polymer conjugates and microarray assays - A method of detection comprising a conjugate of a randomly and asymmetrically branched dendritic polymer. | 03-17-2011 |
20110065091 | Coronavirus, Nucleic Acid, Protein, and Methods for the Generation of Vaccine, Medicaments and Diagnostics - A new coronavirus is disclosed herein with a tropism that includes humans. Means and methods are provided for diagnosing subjects (previously) infected with the virus. Also provided are among others vaccines, medicaments, nucleic acids and specific binding members. | 03-17-2011 |
20110065092 | USE OF NONVIABLE PARTICLES COMPRISING AN INTERNAL CONTROL (IC) NUCLEIC ACID - The present invention relates to the use of non-viable particles (and in particular liposome particles, particles which are in the form of a viral protein coat, non-viable genetically modified organisms or particles made of synthetic polymers), comprising an internal control (IC) nucleic acid sequence as an internal control in nucleic acid-based analysis. The present invention further relates to non-viable particles comprising an IC nucleic acid and kits for carrying out the methods and uses of the invention. | 03-17-2011 |
20110065093 | NOVEL TOOL FOR THE ANALYSIS OF NEURAL CIRCUITS - The application relates to an isolated transsynaptic virus expressing an exogenous fluorescent activity sensor. Preferably, the transsynaptic virus is a rhabdovirus, e.g. rabies virus, or a herpesvirus, preferably an alphaherpesvirus, e.g. pseudorabies virus. The fluorescent exogenous activity sensor used in the transsynaptic virus can be a fluorescent protein Ca | 03-17-2011 |
20110065094 | METHOD AND KIT FOR DETECTION/IDENTIFICATION OF VIRUS-INFECTED CELL - Disclosed is a means for specifically detecting and identifying a virus-infected cell in a floating cell system by a simple manipulation and with high sensitivity. A virus-infected cell can be detected and identified by the following steps (1) to (5): (1) adding a first labeled antibody which has been labeled with a first labeling substance and is directed against a cell surface antigen specific to a target cell to a sample and allowing the mixture to react; (2) immobilizing a protein in the presence of an RNAstabilizing agent; (3) treating the protein with a surfactant; (4) adding a labeled nucleic acid probe for a nucleic acid specific to a target virus to cause the hybridization; and (5) detecting a cell labeled with both the first labeled antibody and the labeled nucleic acid probe by flow cytometry. | 03-17-2011 |
20110065095 | ANTI-(INFLUENZA A VIRUS SUBTYPE H5 HEMAGGLUTININ) MONOCLONAL ANTIBODY - A method of immunoassay of H5 subtype influenza A virus by which the virus can be accurately assayed even in cases where a certain level of mutation has occurred in the H5 subtype influenza A virus, and a kit therefor, and a novel anti-H5 subtype influenza A virus monoclonal antibody which can be used for the immunoassay are disclosed. The antibody or an antigen-binding fragment thereof of the present invention undergoes antigen-antibody reaction with hemagglutinin of H5 subtype influenza A virus, and the corresponding epitope of the antibody or an antigen-binding fragment thereof is located in a region other than the receptor subdomain (excluding C-terminal region thereof consisting of 11 amino acids), which antibody or an antigen-binding fragment thereof does not have neutralizing activity against the influenza A virus. | 03-17-2011 |
20110065096 | VIRAL DIAGNOSTIC METHOD AND WELL FOR USE IN SAME - The present invention relates to a single flat-based well suitable for use in a viral diagnostic method. More particularly, the well has a planar or flat base, as opposed to a curved base. The invention also relates to a viral diagnostic method that employs such single wells. In an embodiment of this method a specially developed tissue culture medium supplemented with hormones and enzymes is employed. | 03-17-2011 |
20110065097 | Apparatus and Method of Contaminant Detection for Food Industry - The present invention is a method and apparatus for contaminant detection in the food industry. Particularly, the method and apparatus involve collecting air samples containing aerosolized contaminate particles from a foodstuff and analyzing the sample for presence of a contaminate. Aerosol lab-on-a-chip and/or electronic nose devices are utilized for the detection of contaminant particles. | 03-17-2011 |
20110065098 | METHODS AND REAGENT KITS FOR IMPROVING ACCURACY OF SAMPLE CLASSIFICATION - The present invention relates to methods for increasing the accuracy of sample classification characterized by the detection of the protein YKL-40 and the protein MASP2 in the samples and methods for determining the efficacy of a drug in treating a cancer in an individual, as well as reagent kits for the same uses. | 03-17-2011 |
20110065099 | METHOD OF PRETREATING SPECIMEN AND IMMUNOASSAY USING THE SAME - The present invention provides a method of pretreating a specimen, which allows measurement according to an immunoassay to be carried out on a specimen from nasal secretion while preventing non-specific reactions. According to this method, the specimen from nasal secretion is treated with a protease beforehand and then an immunoassay is performed. As the protease, it is preferable to use semi-alkaline protease (EC 3.4.21.63). Furthermore, it is preferable that a substance to be pretreated by the pretreatment method according to the present invention is an influenza virus contained in the specimen from nasal secretion. The immunoassay preferably is an immunoagglutination assay. Examples of the immunoagglutination assay include a turbidimetric immunoassay, a latex turbidimetric immunoassay, and a latex agglutination assay that is performed on a slide glass. | 03-17-2011 |
20110070574 | METHOD FOR VIRUS DETECTION - A method of determining the concentration of a virus or antigen thereof in a sample comprises the steps of: providing a sensor surface having immobilized thereto a virus antigen or a virus antigen analogue, mixing the sample with a known amount of antibody to the virus antigen to obtain a predetermined concentration of antibody to the antigen in the sample mixture, contacting the sample mixture with the sensor surface to bind free antibody in the mixture to the sensor surface, measuring the response of the sensor surface to the binding of free antibody, and determining the concentration of the virus or antigen in the sample from a calibration curve prepared by measuring the responses obtained for mixtures containing the predetermined concentration of antibody and different concentrations of virus. | 03-24-2011 |
20110076670 | DETECTION OF ANALYTES VIA NANOPARTICLE-LABELED SUBSTANCES WITH ELECTROMAGNETIC READ-WRITE HEADS - A first set of antibodies are bonded to a substrate, and are exposed to and bonded with target antigens. A second set of antibodies are bonded to nanoparticles, and the nanoparticle labeled antibodies are exposed to the targeted antigens. An electromagnetic write-head magnetizes the nanoparticles, and then a read-sensor detects the freshly magnetized nanoparticles. The substrate comprises a flexible film or a Peltier material to allow selective heating and cooling of the antigens and antibodies. Nanoparticles of different magnetic properties may be selectively paired with antibodies associated with different antigens to allow different antigens to be detected upon a single scan by the read-sensor. | 03-31-2011 |
20110076671 | USE OF SYNTHETIC PEPTIDE DERIVED FROM ZEBRA PROTEIN FOR THE IN VITRO DIAGNOSIS OF THE EPSTEIN-BARR VIRUS (EBV) REACTIVATION - Method of using polypeptide derived from ZEBRA protein, or variants or isoforms of the polypeptide for the in vitro and ex vivo screening of the EBV virus reactivation in a biological sample of a subject afflicted by a pathology associated with EBV infection. | 03-31-2011 |
20110076672 | Phage-Mediated Bioluminescent Detection of Yersinia Pestis - The present disclosure relates to compositions, methods, systems and kits for the detection of microorganisms of the | 03-31-2011 |
20110081642 | Selection of B Cells with Specificity of Interest: Method of Preparation and Use - The present invention is related to the fields of molecular biology, virology, immunology and medicine. The invention provides methods using a composition comprising an ordered and repetitive antigen or antigenic determinant array for visualization and selection of B cells specific for the antigen. These B cells are useful for the production of monoclonal antibodies used for therapy, diagnostic or research purposes. | 04-07-2011 |
20110081643 | Flow Focusing Method and System for Forming Concentrated Volumes of Microbeads, and Microbeads Formed Further Thereto - In a method and system for forming concentrated volumes of microbeads, a polymer solution and/or suspension includes a polymer dissolved and/or dispersed in a medium. Streams of a focusing fluid and of the polymer solution and/or suspension flow towards a fluid bath, and into intersection with one another, so &s to focus the polymer solution and/or suspension. The polymer solution and/or suspension stream forms microbeads in the fluid bath. Some of the focusing fluid is drawn from the fluid bath, so as to concentrate the microbeads in die fluid bath. The system includes a flow focusing apparatus and a liquid-containing cell. The focusing apparatus includes polymer and focusing nozzles. The cell contains the fluid bath and has an outlet port, through which the focusing fluid is drawn from the fluid bath. | 04-07-2011 |
20110081644 | CELL LINE STABLY EXPRESSING MUTATED ONCOPROTEIN E6 AND METHOD OF SCREENING ANTICANCER COMPOUND OF UTERINE CERVICAL CANCER USING THE SAME - Disclosed are a cell line that expresses protein of Seq. No. 1, and a method for screening an anticancer compound of uterine cervical cancer by using the same. The stable cell line that expresses oncoprotein E6 of a human papillomavirus type 16 variant strain is used to determine a difference in amounts of expression of tumor suppressor genes, such as p53, between E6 protein of the reference strain and E6 protein of a variant strain, thereby screening an anticancer compound of uterine cervical cancer, etc. Further, it is possible to develop an anticancer agent of uterine cervical cancer. | 04-07-2011 |
20110081645 | COMPOSITIONS AND METHODS FOR DETECTING HEPATITIS B VIRUS - Compositions, methods and kits for detecting viral nucleic acids. Targets that can be detected in accordance with the invention include HBV and/or HIV-1 and/or HCV nucleic acids. Particularly described are oligonucleotides that are useful as hybridization probes and amplification primers that facilitate detection of very low levels of HBV nucleic acids. | 04-07-2011 |
20110081646 | DENGUE VIRUS ASSAY - Nucleic acid assays for detecting nucleic acids of Dengue virus serotypes 1-4. | 04-07-2011 |
20110086338 | Bacteriophage/Quantum-Dot (Phage-QD) Nanocomplex to Detect Biological Targets in Clinical and Environmental Isolates - The invention is related to a non-biotinylated bacteriophage that comprises a nucleic acid sequence encoding a biotinylation domain, a complex that comprises a biotinylated bacteriophage and a biotin-specific ligand conjugated bioconjugate, and a method of detecting a bacterial cell in a sample comprising contacting the sample with a non-biotinylated bacteriophage that comprises a nucleic acid sequence encoding a biotinylation domain, wherein the bacteriophage is specific to the bacterial cell. | 04-14-2011 |
20110086339 | ELIMINATION OF PATHOGENIC INFECTION IN FARMED ANIMAL POPULATIONS - Animal husbandry has always been susceptible to the ravages of pathogenic infections. Poultry flus and cattle diseases are but two examples that have dire consequences for animals and adversely affect the economic fortunes of farmers. A testing and culling methodology is presented that can eliminate pathogens from an infected herd. The sensitivity of PCR to detect very low levels of nucleic acid of an infecting pathogen is utilized to identify infected animals. In addition, it has been discovered that PCR analysis of manure samples can accurately identify infected animals and offspring for those species that consume placental remains after birth. Mink Aleutian Disease Virus (mADV) is one of several deadly DNA parvoviruses that can quickly reach epidemic proportions in a mink herd. PCR primers have been developed that generate amplicons to detect and identify the mADV. In addition, a previously unidentified strain of mADV has been discovered, genomically sequenced, and substantially detailed. | 04-14-2011 |
20110086340 | METHODS, DEVICES, KITS AND COMPOSITIONS FOR DETECTING ROUNDWORM - Methods, devices, kits and compositions for detecting the presence or absence of roundworm in a mammalian sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm. Confirmation of the presence or absence of roundworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated. | 04-14-2011 |
20110086341 | FLUORINATED RESORUFIN COMPOUNDS AND THEIR APPLICATION - The invention provides novel fluorinated resorufin compounds that are of use in a variety of assay formats. Also provided are methods of using the compounds and kits that include a compound of the invention and instructions detailing the use of the compound in one or more assay formats. | 04-14-2011 |
20110091865 | MEASURING MULTIPLE ANALYTES OVER A BROAD RANGE OF CONCENTRATIONS USING OPTICAL DIFFRACTION - The invention relates to method, devices, and kits for measuring multiple analytes in a sample having a broad range of concentrations using optical diffraction. Devices, methods, and kits useful for monitoring and diagnosing diabetes, cardiovascular disease, thyroid disease, hormone-related conditions, and sepsis are also described. | 04-21-2011 |
20110091866 | DETECTION OF POLYOMAVIRUS - Methods and kits are provided for testing for the presence or absence of a polyomavirus, such as BKV, in a sample. The methods and kits are useful for quantifying BKV and differentiating BKV from JCV. | 04-21-2011 |
20110091867 | METHOD TO IDENTIFY AND PREDICT DISEASE PROGRESSION OF HUMAN PAPILLOMA VIRUS-INFECTED LESIONS - The present invention provides a method for distinguishing benign human papilloma virus (HPV)-infected tissue from HPV-related lesions that have undergone malignant transformation. In one embodiment, the invention comprises a simple histochemical staining method and details a novel process for examining HPV-infected cells by determining susceptibility to enzymatic DNA digestion. Residual virion-associated DNA is seen only in benign HPV-infected lesions, while absence of residual DNA is seen with malignant transformation. In another embodiment, the invention comprises immunohistochemical assay methods for examining HPV-infected cells, utilizing antibodies to HPV L1 proteins. These methods can be used to predict biologic behavior of HPV-infected lesions. The invention can improve current cervical cancer screening programs, and improve clinical management of patients by defining malignant potential of HPV-infected tissue more accurately. | 04-21-2011 |
20110091868 | NOVEL ANTIGEN CONSTRUCTS USEFUL IN THE DETECTION AND DIFFERENTIATION OF ANTIBODIES TO HIV - Isolated HIV-1 Group O env polypeptides obtained from the HIV-1 isolate HAM112 are claimed, as well as (a) antigen constructs comprising fusions of one or more of each of HIV-1 Group O env polypeptides and HIV-1 Group M env polypeptide and (b) further antigen constructs containing additional Group O sequences and especially the gp41 IDR of isolate HAM112. Also claimed are polynucleotide sequences encoding the above, expression vectors comprising the same, host cells transformed thereby, and immunoassay methods and kits utilizing the antigen constructs of the invention. | 04-21-2011 |
20110091869 | METHOD FOR CULTURE OF STEM CELL - The present invention enables efficient suspension culture of stem cells in a serum-free medium by comprising a step for quickly forming a homogenous aggregate of stem cells, and provides a method of selectively inducing the differentiation of nerves from a stem cell, a method of forming a cerebral cortical nerve network in vitro, and a method of producing a steric structure of a brain tissue in vitro, as well as a method of producing hypothalamic neuron progenitor cells, comprising culturing pluripotent stem cells as a suspended aggregate in a serum-free medium that substantially does not contain a Nodal signal promoter, a Wnt signal promoter, an FGF signal promoter, a BMP signal promoter, retinoic acid and an insulin, and isolating hypothalamic neuron progenitor cells from the culture. | 04-21-2011 |
20110097702 | Methods and compositions for in situ detection of microorganisms on a surface - Compositions and methods for in situ detection of one or more target microorganisms on a surface and preferably on a hard surface. Compositions and methods of the invention are based on the specificity of certain bacteriophage for target microorganisms. Bacteriophage are modified to express detectable biomarkers in the presence of the target microorganism, the detectable markers being detectable on the surface being tested using a portable detection device. Only living microorganisms are detected using the methods and compositions of the invention. | 04-28-2011 |
20110097703 | METHOD FOR CARRYING OUT A QUALITATIVE PRELIMINARY INSTANT DIAGNOSIS OF ONCOLOGIC DISEASES - The invention relates to medicine, in particular to a method for carrying out a preliminary instant diagnosis of oncologic diseases. The method involves sampling a patient's blood, mixing the sample with a reagent and recording reaction results. A supernatant liquid, which is produced by cultivating the finite cell line of a porcine embryo kidney culture in anaerobic conditions, is used as a reagent. The presence of oncologic disease is determined according to a positive reaction of erythrocytes and a negative or positive reaction of a citrated blood with the reagent. The inventive method makes it possible to make a preliminary conclusion about the presence of oncologic disease within a short time. | 04-28-2011 |
20110097704 | COMPOSITIONS FOR USE IN IDENTIFICATION OF PICORNAVIRUSES - The present invention provides oligonucleotide primers, compositions, and kits containing the same for rapid identification of viruses which are members of the Picornaviridae family by amplification of a segment of viral nucleic acid followed by molecular mass analysis. | 04-28-2011 |
20110097705 | Surface-assisted hemagglutination and hemagglutination inhibition assays - Hemagglutination (HA) and hemagglutination inhibition (HAI) functional assays remain important instruments of analysis of virus-cell interaction and protecting efficacy of virus-specific antibodies and sera. However, they demonstrate limited sensitivity towards many viruses, and require significant volumes of viruses, erythrocytes, sera, and antibodies. The present invention comprises new and significantly more sensitive versions of the HA and HAI assays based on observing agglutination on activated surfaces of specifically opsonized plates and ELISA plates rather than in solution. A version of the new assay that uses ELISA plates additionally allows characterizing the affinity of functional antibodies in the tested sera and fluids, which is not possible in the classical HAI assay. The methods of the present invention can also be used to improve the sensitivity of agglutination methods based on latex beads and to develop agglutination methods using target cells other than erythrocytes. | 04-28-2011 |
20110097706 | MICRO-REACTOR FOR OBSERVING PARTICLES IN A FLUID - The invention relates to a micro-reactor for observing small particles, cells, bacteria, viruses or protein molecules in a fluid. The micro-reactor shows a first channel formed between two layers for containing the fluid, with an inlet and an outlet, the two layers separated by a first distance. A likewise second channel with an inlet and an outlet is placed adjacent to the first channel. A gap connects the first channel and the second channel, at the gap at least one layer showing a window transparent to the method of inspection and at the window the two layers being separated by a very small distance of, for example, 1 μm or less. | 04-28-2011 |
20110097707 | RNAi Agents Comprising Universal Nucleobases - One aspect of the present invention relates to an oligonucleotide agent comprising at least one universal nucleobase. In certain embodiments, the universal nucleobase is difluorotolyl, nitroindolyl, nitropyrrolyl, or nitroimidazolyl. In a preferred embodiment, the universal nucleobase is difluorotolyl. In certain embodiments, the oligonucleotide is double-stranded. In certain embodiments, the oligonucleotide is single-stranded. Another aspect of the present invention relates to a method of altering the expression level of a target in the presence of target sequence polymorphism. In a preferred embodiment, the oligonucleotide agent alters the expression of different alleles of a gene. In another preferred embodiment, the oligonucleotide agent alters the expression level of two or more genes. In another embodiment, the oligonucleotide agent alters the expression level of a viral gene from different strains of the virus. In another embodiment, the oligonucleotide agent alters the expression level of genes from different species. | 04-28-2011 |
20110097708 | ASSESSMENT OF HUMAN PAPILLOMA VIRUS-RELATED DISEASE - This invention provides novel methods for assessing HPV infection. Gene expression levels are used to assess the progression of HPV infection from benign to malignant growth. Also provided are kits for carrying out the methods of this invention. | 04-28-2011 |
20110104657 | METHOD OF DETECTING MALIGNANCY OF NASOPHARYNGEAL CARCINOMA AND A NASOPHARYNGEAL CARCINOMA MALIGNANCY BIOMARKER - A method of detecting malignancy of nasopharyngeal carcinoma and a nasopharyngeal carcinoma malignancy biomarker are disclosed. Firstly, a specimen is obtained from a testee. Next, the specimen is tested for its MIP-3α expression level. Then, the MIP-3α expression level of the specimen is compared with that of a control. Finally, the malignancy of nasopharyngeal carcinoma is determined according to a relative MIP-3α expression level between the specimen and the control. | 05-05-2011 |
20110104658 | Synthetic Microfluidic Blood-Brain Barrier - An apparatus and method for assaying blood-brain barrier properties for drug and drug delivery vehicle screening comprising of a microfluidic apparatus with gaps separating lumen and tissue space enabling formation of tight junctions similar to in vivo conditions using endothelial cells and brain cells. | 05-05-2011 |
20110104659 | DEVICE FOR BIOCHEMICAL PROCESSING AND ANALYSIS OF A SAMPLE - A device including a sample compartment, a coil and an arm for mechanical manipulation of a sample vessel placed in the sample compartment and containing a sample is described. In at least one embodiment, the coil is surrounding the sample compartment and the sample compartment has an opening for insertion and removal of the sample vessel. A method, using the device according to at least one embodiment of the invention for detection of magnetic permeability, relative magnetic permeability or relative magnetic susceptibility, is also described. | 05-05-2011 |
20110104660 | MAL, A MOLECULAR DIAGNOSTIC MARKER FOR HPV-INDUCED INVASIVE CANCERS AND THEIR HIGH-GRADE PRECURSOR LESIONS - The inventors now have developed a (molecular) diagnostic marker based on MAL alterations, in particular reduced MAL mRNA and protein expression as well as MAL promoter hypermethylation, to identify human papillomavirus (HPV)-induced high-grade precancerous lesions such as premalignant cervical lesions of invasive cervical cancer, and high-risk human papillomavirus (HPV)-induced precursor lesions of non-cervical invasive cancers within, cell material obtained via scraping, lavage or by other means and/or tissue. In particular, the present invention relates to the use of the MAL gene (including its promoter) and the gene products thereof as marker for HPV-induced high-grade premalignant lesions, allowing early detection and better treatment option for the individual patient. | 05-05-2011 |
20110104661 | DEGRADABLE POLYACRYLAMIDE GEL - A degradable polyacrylamide gel for analyzing or separating at least one macromolecule in a sample using electrophoresis includes a polyacrylamide cross-linked with at least one degradable cross-linker having a ketal or acetal group with the formula (I), wherein R | 05-05-2011 |
20110104662 | Methods of Detecting Inhibitors of VIF-Mediated APOBEC3G Degradation and HIV - The invention comprises methods and cell lines for assaying APOBEC3G degradation and methods for identifying inhibitors of APOBEC3G degradation. The invention also provides methods of identifying inhibitors of HIV infection. The methods of the invention are useful for identifying inhibitors of viral infection, in particular, the methods of the invention are useful for treating retroviral infection. | 05-05-2011 |
20110111386 | CERVICAL CELL COLLECTION METHOD - The present invention relates to sample collection and preservation. More specifically, the invention provides methods for the collection and preservation of at least one nucleic acid molecule in a test sample comprising one or more cells or tissues obtained from the cervix of a subject. | 05-12-2011 |
20110111387 | HIGH-EFFICIENCY VIABLE SAMPLER FOR ULTRAFINE BIOAEROSOLS - Exemplary embodiments provide bioaerosol detection systems and methods for detecting bioaerosols. In one embodiment, the bioaerosol detection system can include a humidifier to increase the humidity of a continuously flowing sample volume of a bioaerosol sample using a biologically compatible liquid medium, and an amplifier to deposit vapor on the bioaerosol sample for a particle size amplification process. Bioaerosol(s) can thus be detected and sampled while simultaneously maintaining their viability. The disclosed bioaerosol detection systems and the methods can provide high efficiency for sampling and detecting ultrafine bioaerosol(s) such as viruses and proteins, which can be smaller than 0.3 μm in diameter and can be as small as 20 nm. | 05-12-2011 |
20110111388 | METHOD FOR THE SENSITIVE DETECTION OF POLYAMINO ACIDS AND OTHER MACRO-MOLECULES - The invention relates to a method for the detection of an analyte containing polyamino acid. The object of the invention is to detect polyamino acids in a highly sensitive manner. The object is achieved in that an LM is coupled to a protein after chemical activation, and is complexed using a lanthanoid ion. In order to detect polyamino acids, the time resolved fluorescence measurement is utilized after electrophoretic separation. The detection limit is 0.5 pg per spot (bovine serum albumin), wherein the linear region extends across six orders of magnitude. The invention also relates to analytes containing nucleic acid. | 05-12-2011 |
20110111389 | RAPID GENOTYPING ANALYSIS FOR HUMAN PAPILLOMAVIRUS AND THE DEVICE THEREOF - The present invention discloses methods and devices for rapid genotyping. In one embodiment, the present invention is applied to human papillomavirus (HPV) genotyping, comprising the use of viral genotype-specific-oligonucleotide probes, reversed-dot-blotting genotype array format and flow through hybridization process, thereby providing a more efficient, faster and less expensive method for HPV genotyping. The genotyping method further comprises the use of generic probes to expand the detection of HPV genotypes. | 05-12-2011 |
20110111390 | METHOD FOR IN VITRO DETECTION AND/OR QUANTIFICATION AND/OR IDENTIFICATION OF INFECTIOUS COMPOUNDS IN A BIOLOGICAL MATERIAL - Method for in vitro detection and/or quantification and/or identification of infectious compounds present in a fluid medium M constituting a biological material, in which method a suspension of microbeads of solid polymer material capable of binding proteins is prepared; the microbeads are loaded with β2GPI proteins by coupling with a sufficient amount of β2GPI proteins; said microbeads are brought into contact with the fluid medium M while adding ions of at least one oxidizing metal, so as to bind the infectious compounds to the β2GPI proteins; the microbeads thus prepared are separated from their suspension medium, so as to obtain a residue; and the infectious compounds of the residue are detected and/or quantified and/or identified. | 05-12-2011 |
20110111391 | NEWLY IDENTIFIED HUMAN RHINOVIRUS OF HRV-C AND METHODS AND KITS FOR DETECTING HRV-CS - The characterization of a new strain of human rhinovirus of genetic group C(HRV-C) as well as methods and kits for detecting the presence of HRV-C by PCR amplification are provided. | 05-12-2011 |
20110111392 | INTEGRATED ENHANCED CHEMILUMINESCENCE BIOSENSORS - A method and apparatus for determining the concentration of an analyte in a sample is provided. This method involves combining enhanced chemiluminescence with microchip capillary electrophoresis or microchip liquid chromatography. | 05-12-2011 |
20110117538 | BIOREACTORS FOR FERMENTATION AND RELATED METHODS - Bioreactors suitable for housing a predetermined volume of liquid comprising nutrient medium and biological culture comprising: (a) a container having at least one interior wall; (b) at least one nutrient medium inlet; (c) at least one liquid outlet; (d) at least one gas inlet; (e) at least one gas outlet; and (f) at least one cylindrical sparging filter attached to the at least one gas inlet, wherein the sparging filter comprises a plurality of pores along its axis which permit gas to be emitted radially from the sparging filter into the liquid, wherein the diameter of the plurality of pores does not exceed about 50 μm, and wherein the orientation of the at least one sparging filter within the container provides for immersion of the plurality of pores within the liquid and substantially uniform distribution of emitted gas throughout the liquid, and related methods of using said bioreactors to prepare various biological products. | 05-19-2011 |
20110117539 | Detection of an Analyte in a Sample - There is provided mechanisms for the detection of an analyte in a sample. The mechanisms utilize at least a first measurement channel comprising a detection reactant corresponding to the analyte to be detected, and at least a microstructure associated with the first measurement channel. When the mechanisms are in use, the sample is introduced into the first measurement channel and propagated by way of the first measurement channel towards the microstructure. If the analyte, if it is present in the sample, the analyte interacts with the detection reactant to form a networked product, and the microstructure is configured to filter the networked product. | 05-19-2011 |
20110117540 | Highly Simplified Lateral Flow-Based Nucleic Acid Sample Preparation and Passive Fluid Flow Control - Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays. | 05-19-2011 |
20110117541 | Method for Screening of Agents for the Prevention of Hepatitis C Virus Infection with Cell Culture Tool - The invention relates to an improved method of screening of anti-HCV agents that may have an efficacy for prevention of hepatitis C virus. The method involves the isolation and cryopreservation of HCV-infected hepatocytes from multiple infected individuals. The isolated and cryopreserved hepatocytes are stored in a cryopreservation bank made up of HCV-infected hepatocytes representing the different genotypes of HCV. These stored hepatocytes then are co-cultured in a culture medium with uninfected hepatocytes, and anti-HCV screening of the hepatocytes is done by subjecting HCV infected hepatocytes and uninfected hepatocytes in parallel to the actions of different anti-HCV compounds at various concentrations. An effective anti-HCV agent will lead to prevention of increase in concentration of HCV content of uninfected cells in the co-culture. | 05-19-2011 |
20110117542 | REAL TIME ELECTRONIC CELL SENSING SYSTEM AND APPLICATIONS FOR CYTOTOXICITY PROFILING AND COMPOUND ASSAYS - The invention provides methods of investigating a mechanism of action of a compound, which includes providing a device for monitoring cell-substrate impedance; attaching the device to an impedance analyzer; adding cells to two or more wells; adding a test compound to at least one of the wells and providing at least one control well; monitoring impedance of the wells to obtain a series of impedance measurements; plotting impedance measurements to obtain impedance curves and comparing the impedance curves to determine a time frame at which the test compound has a significant effect on cell growth or behavior. Determining the time frame provides information on changes in cell status in response to the test compound, including cell attachment or adhesion status, cell growth or proliferation status, the number of viable or dead cells, cytoskeletal organization or structure, and the number of cells going through apoptosis or necrosis. | 05-19-2011 |
20110123977 | METHOD FOR TAKING A PLURALITY OF SAMPLES - The invention provides a method for taking a plurality of samples ( | 05-26-2011 |
20110123978 | HUMAN PAPILLOMA VIRUS (HPV) DETECTION USING NUCLEIC ACID PROBES, MICROBEADS AND FLUORESCENT-ACTIVATED CELL SORTER (FACS) - The present invention relates generally to the field of diagnostic and detection assays. More particularly, the present invention provides methods, and reagents including biochips for detecting the presence of, or distinguishing between, one or more analytes in a sample. | 05-26-2011 |
20110123979 | DETECTION OF MICROORGANISMS - A method of collecting, detecting and enumerating microorganisms in a fluid comprising subjecting a sample of the fluid to dielectrophoresis and collecting the microorganisms onto a microelectrode, scanning the microelectrode using a scanning laser and determining the number of microorganisms present on the microelectrode. Alternatively, the microorganisms may be spun onto a substrate which has been pre-treated with a polycationic electrolyte. | 05-26-2011 |
20110129813 | 2'-BRANCHED NUCLEOSIDES AND FLAVIVIRIDAE MUTATION - The present invention discloses a method for the treatment of Flaviviridae infection that includes the administration of a 2′-branched nucleoside, or a pharmaceutically acceptable prodrug and/or salt thereof, to a human in need of therapy in combination or alternation with a drug that directly or indirectly induces a mutation in the viral genome at a location other than a mutation of a nucleotide that results in a change from serine to a different amino acid in the highly conserved consensus sequence, XRX | 06-02-2011 |
20110129814 | PROCESS AND SYSTEM FOR DETECTING AND/OR QUANTIFYING BACTERIOPHAGES, USE OF A MICROELECTRONIC SENSOR DEVICE FOR DETECTING SAID BACTERIOPHAGES AND MICROELECTRONIC SENSOR DEVICE FOR CARRYING OUT SAID PROCESS - The present invention relates to a process and system for detecting and/or quantifying bacteriophages capable of infecting a predetermined bacterial host strain, which is based on the surface plasmon resonance technique that takes place on the conductive material surface of an optical sensor device, and also relates to a microelectronic sensor device for carrying out this process and to the use of this microelectronic sensor device for detecting and/or quantifying bacteriophages. | 06-02-2011 |
20110129815 | METHOD FOR PRETREATING SAMPLE FOR DETECTION HCV CORE PROTEIN, REAGENT KIT FOR DETECTION OF HCV CORE PROTEIN, METHOD FOR DETERMINING THE PRESENCE OR ABSENCE OF HEPATITIS C VIRUS IN SAMPLE, AND METHOD FOR IMMUNOASSAY OF HCV - There are provided: a method for pretreating a sample for HCV core protein detection by an immunoassay using particles, which includes treating a sample suspected of containing hepatitis C virus (HCV) with an alkaline material-containing reagent and neutralizing the sample with an acid material-containing reagent, wherein at least one of the reagents contains a reducing agent; a reagent kit for HCV core protein detection; a method for determining the presence or absence of hepatitis C virus in a sample; and a method for immunoassay of HCV. | 06-02-2011 |
20110129816 | DEVICE FOR DETECTION OF INFLUENZA VIRUS - A device for detecting or quantifying influenza viruses in a sample, which comprises a detection region having a human anti-influenza virus nucleoprotein antibody immobilized onto a support, a sample supply region, and a sample-migrating region; and a kit for detecting or quantifying influenza viruses, which comprises a solid phase in which a human anti-influenza virus nucleoprotein antibody is fixed onto a carrier. | 06-02-2011 |
20110136098 | METHODS FOR SIMPLIFYING MICROBIAL NUCLEIC ACIDS BY CHEMICAL MODIFICATION OF CYTOSINES - A method for detecting a microorganism comprising reducing the complexity of a microbial genome or microbial nucleic acid by generating a simplified form of the microbial genome or microbial nucleic acid in which substantially all of the positions naturally occupied by cytosines are occupied by uracil or thymine; and assaying for a microbial specific nucleic acid in the simplified form of the microbial genome or microbial nucleic acid, wherein presence of the microbial specific nucleic acid is indicative of the microorganism. | 06-09-2011 |
20110136099 | Multiplexed Analyses of Test Samples - The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer). The methods described create a nucleic acid surrogate for a non-nucleic acid target, thus allowing the wide variety of nucleic acid technologies, including amplification, to be applied to a broader range of desired targets, especially protein targets. The disclosure further describes aptamer constructs that facilitate the use of aptamers in a variety of analytical detection applications. | 06-09-2011 |
20110136100 | NON-HUMAN ANIMAL DISEASE MODEL FOR HEPATITIS B VIRUS-ASSOCIATED DISEASE - A non-human animal disease model for hepatitis B virus-associated liver disease is disclosed. The animal disease model is transduced with a hepatitis B virus genome in the liver cells thereof and exhibits the following symptoms: hepatitis B viral particles and hepatitis B viral DNA in the serum, hepatitis B virus (HBV) envelope and HBV e proteins in the serum, expression of HBV core and HBV envelope proteins in the liver but not in the kidney, heart, lung, brain, pancreas, spleen, stomach or intestine tissues. The animal disease model may develop hepatocellular carcinoma, exhibiting an elevated level of alanine aminotransferase as compared to a control animal without the hepatitis B virus genome in the liver cells thereof, and liver pathological symptoms such as tumor nodules, dysplasia, inflammatory infiltrates, necrosis and fibrosis. | 06-09-2011 |
20110136101 | Immortalized Human Fetal Liver Cells - The present invention provides immortalized fetal liver cells that express the oncogene Simian virus (SV 40) large T-antigen and specific hepatocyte markers and a method for producing same. | 06-09-2011 |
20110136102 | Surface Impedance Imaging Methods and Apparatuses - Methods and apparatuses for imaging surface impedance. | 06-09-2011 |
20110143332 | METHOD FOR IDENTIFYING MICROORGANISM OR DETECTING ITS MORPHOLOGY ALTERATION USING SURFACE ENHANCED RAMAN SCATTERING (SERS) - The present invention relates to a method for producing a profile identifying a microorganism based on surface enhanced Raman scattering (SERS) and an apparatus thereof. The method comprises: (1) placing the microorganism on a SERS-active substrate; (2) mounting the microorganism with a mounting solution; (3) obtaining a SERS spectrum of the microorganism in step (2); and (4) analyzing the SERS spectrum to produce the profile. | 06-16-2011 |
20110143333 | ANTI-CHIKUNGUNYA MONOCLONAL ANTIBODIES AND USES THEREOF - The present invention relates to the field of arbovirosis caused by Chikungunya virus (CHIK). The present invention specifically concerns anti-CHIK monoclonal antibodies (MAbs), and more specifically anti-CHIK.E2 MAbs and their use as diagnostic products in methods for detecting the presence or absence of a CHIK strain. | 06-16-2011 |
20110143334 | MICROBIOLOGICAL SYSTEMS AND METHODS OF FLUID SAMPLE ANALYSIS - Methods and systems for detecting the presence of a target microorganism in a liquid sample are provided. Methods comprise the steps of passing the liquid sample through a surface filter, placing the surface filter into contact with a culture device, incubating the culture device for a period of time and detecting the presence of a target microorganism. Methods may be used with an automated detection system. | 06-16-2011 |
20110143335 | METHODS AND SYTEMS TO CAPTURE COMPETITIVE MOLECULES - Methods and systems to capture competitive molecules, such as to reduce false positives in an assay. Competitive molecules may be captured in a fluid moving through a portable point-of-care diagnostic assay system. | 06-16-2011 |
20110143336 | MARKER FOR ESTIMATING THE DIAGNOSIS OF CERVICAL ADENOCARCINOMA OR FOR ESTIMATING THE PROGNOSIS OF CERVICAL CANCER - To provide a novel biomarker for estimating the diagnosis of cervical adenocarcinoma or for estimating the prognosis of cervical cancer. An antibody against Villin 1 is employed as a biomarker. | 06-16-2011 |
20110143337 | UNSYMMETRICAL CYANINE DIMER COMPOUNDS AND THEIR APPLICATION - Embodiments of the present invention provide methods and nucleic acid reporter molecules for the detection of nucleic acid in a sample. The nucleic acid reporter molecule comprises two unsymmetrical cyanine monomer moieties, which may be the same or different, that are covalently attached by a linker comprising at least one aromatic, heteroaromatic, cyclic or heterocyclic moiety comprising 3-20 non-hydrogen atoms selected from the group consisting of O, N, S, P and C. The linker may be rigid, relatively flexible or some degree thereof. The unsymmetrical cyanine monomer moieties comprise a substituted or unsubstituted benzazolium moiety and a substituted or unsubstituted pyridinium or quinolinium moiety that is connected by a methine bridge that is monomethine, trimethine or pentamethine. The linkers form the cyanine dimer compounds by attaching to the pyridinium or quinolinium moiety of the monomer moieties. The present nucleic acid reporter molecules find utility in forming a nucleic acid-reporter molecule complex and detecting the nucleic acid. In particular, present nucleic acid reporter molecules with a rigid linker and monomer moieties with a monomethine bridge find utility in detecting RNA in the presence of DNA. | 06-16-2011 |
20110151429 | Bioprobe, Method of Preparing the Bioprobe, and Analysis Apparatus and Method Using the Bioprobe - The present invention relates to a bioprobe including a substrate and inorganic nanoparticles attached to the surface of the substrate, a method of preparing the bioprobe, and an analysis apparatus and method using the bioprobe. In the bioprobe according to the present invention, inorganic nanoparticles introduced to the substrate serve as a linker to which a target-specific substance such as an antibody can be bound, and they also increase the surface area of the substrate, thus increasing a surface area where a target substance to be detected can contact the substrate. In this regard, the bioprobe can be effectively used for detection, dosing, or analysis of various biomolecules or other chemical substances. | 06-23-2011 |
20110151430 | VIRUS-SPECIFIC miRNA SIGNATURES FOR DIAGNOSIS AND THERAPEUTIC TREATMENT OF VIRAL INFECTION - The present invention related to miRNA signatures and diagnostic and therapeutic applications of miRNA signatures. The miRNA signatures are defined by a test sample miRNA profile relative to an appropriate control miRNA profile. In some embodiments, the test sample is a sample isolated, obtained or derived from a virus-infected cell or organism. The present invention further relates to the use of miRNA signatures in the identification of druggable targets and antiviral agents. Kits and compositions are also provided. | 06-23-2011 |
20110151431 | DETECTION OF XENOTROPIC MURINE LEUKEMIA VIRUS - Methods of detecting, diagnosing, monitoring or managing an XMRV-related neuroimmune disease such as chronic fatigue syndrome or XMRV-related lymphoma such as mantle cell lymphoma in a subject are disclosed. These methods comprise determining presence, absence or quantity an XMRV nucleic acid in a sample from a subject. | 06-23-2011 |
20110151432 | METHODS AND SYSTEMS TO COLLECT AND PREPARE SAMPLES, TO IMPLEMENT, INITIATE AND PERFORM ASSAYS, AND TO CONTROL AND MANAGE FLUID FLOW - Methods and systems to related to sample collection, assays, and fluid control and management, including methods and systems to implement hand-held portable assays, to activate an assay system, to collect and prepare samples, to capture antibodies, and to trap or capture gas bubbles. Methods and systems disclosed herein, and portions thereof, may be implemented alone and/or in various combinations with one another. | 06-23-2011 |
20110151433 | METHODS FOR PRODUCTION OF UNSTRUCTURED RECOMBINANT POLYMERS AND USES THEREOF - The present invention provides unstructured recombinant polymers (URPs) and proteins containing one or more of the URPs. The present invention also provides microproteins, toxins and other related proteinaceous entities, as well as genetic packages displaying these entities. The present invention also provides recombinant polypeptides including vectors encoding the subject proteinaceous entities, as well as host cells comprising the vectors. The subject compositions have a variety of utilities including a range of pharmaceutical applications. | 06-23-2011 |
20110151434 | ADENO-ASSOCIATED VIRUS (AAV) SEQUENCES AND ISOLATING NOVEL SEQUENCES IDENTIFIED THEREBY - A method for detecting and isolating AAV sequences in a sample of DNA obtained from tissue or cells is provided, which sample contains DNA and proviral AAV. The method involves subjecting the sample containing DNA to amplification via polymerase chain reaction (PCR) using a first set of primers which specifically amplify a first AAV region. The first AAV region is characterized by having at least 250 nucleotides of AAV capsid nucleic acid sequence, a variable sequence flanked by a sequence of at least 18 nucleotides at the 5′ end of the first AAV region and a sequence of at least 18 nucleotides at the 3′ end of the first AAV region. Each of the 5′ and 3′ at least 18 nucleotides is the same over at least 9 consecutive nucleotides relative to corresponding sequences in an alignment of at least two AAV serotypes. Each of the sets of primers consist of a 5′ primer and a 3′ primer. The method is further useful for identifying AAV sequences in the sample by the presence of amplified proviral AAV sequences. | 06-23-2011 |
20110151435 | NOVEL ASSAYS FOR DETECTING ANALYTES IN SAMPLES AND KITS AND COMPOSITIONS RELATED THERETO - The present invention provides methods of detecting analytes using particles having different physico-chemical properties, such as buoyancy, size, density, spectral characteristics, and/or binding properties, in solution-based sandwich assays and solution-based competition assays. The methods can be performed using rotors and bench-top centrifuges and provide for rapid, qualitative and quantitative detection of analytes. The present invention also provides kits that can be used to perform the methods, and mixtures containing particles suitable for the methods. | 06-23-2011 |
20110151436 | COMPOSITIONS AND METHODS FOR THE DETECTION OF HIV-1/HIV-2 INFECTION - This invention relates to compositions and methods or the detection of immunodeficiency virus infection, especially immunodeficiency virus-1 (HIV-1) infection. The invention particularly concerns compositions and methods that may be used in HIV vaccine recipients whose sera may contain vaccine-generated anti-HIV-1 antibodies. | 06-23-2011 |
20110151437 | COMPOSITIONS FOR USE IN IDENTIFICATION OF ADVENTITIOUS VIRUSES - The present invention provides compositions, kits and methods for rapid identification and quantification of adventitious contaminant viruses by molecular mass and base composition analysis. | 06-23-2011 |
20110159477 | PRODUCTS AND ANALYTICAL METHOD - The present invention describes product and analysis method for analysis of a substance, a virus, a bacteria or a cell which binds to carbohydrate structures. One or more carbohydrates, or carbohydrate derivatives, is bound to spherical or non-spherical polymer beads (for example microspheres or nanospheres). Formed carbohydrate-polymer beads or carbohydrate-polymer particles, can bind to with more or less specific affinity to the substance, bacteria, virus or cell which shall be detected. The carbohydrate beads is contacted with a sample containing the substance, bacteria, virus or cell (completely or partially isolated, or not purified, in a non-diluted or diluted solution). The resulting mixture is optionally added to a gel, or a column containing a gel, through which the resulting mixture (of carbohydrate beads or carbohydrate particles bound to the substance, virus, bacteria or cell), is allowed to migrate. Detection of aggregates is made visually or using apparatus for detection. | 06-30-2011 |
20110165554 | METHODS AND BIOMARKERS FOR DIAGNOSING AND MONITORING PSYCHOTIC DISORDERS - A method of diagnosing or monitoring a psychotic disorder, or predisposition thereto, comprises measuring, in a sample taken from a subject, the levels of one or more peptide biomarkers listed herein. | 07-07-2011 |
20110165555 | Capillary-Column-Based Bioseparator/Bioreactor with an Optical/Electrochemical Detector for Detection of Microbial Pathogens - The present invention is directed to satisfying the need to detect microbial contamination of food products. The described bioseparator/bioreactor coupled with an optical/electrochemical biosensor was able to specifically detect | 07-07-2011 |
20110165556 | SYSTEM AND METHOD FOR DETECTION OF HIV TROPISM VARIANTS - An embodiment of a method for detecting low frequency occurrence of one or more HIV sequence variants associated with drug resistance is described that comprises the steps of: generating cDNA species from each RNA molecule in an HIV sample population; amplifying at least one first amplicon from the cDNA species, wherein each first amplicon comprises a plurality of amplified copies and is amplified with a pair of nucleic acid primers that define a locus of the first amplicon; clonally amplifying the amplified copies of the first amplicons to produce a plurality of second amplicons wherein a plurality of the second amplicons comprise an immobilized population of substantially identical copies from one of the amplified copies of first amplicons; determining a nucleic acid sequence composition of the substantially identical copies from at least 100 of the immobilized populations in parallel on a single substrate; and detecting one or more sequence variants that occur at a frequency of 5% or less in the nucleic acid sequence composition of the at least 100 immobilized populations; and correlating the detected sequence variants with variation associated with HIV tropism. | 07-07-2011 |
20110165557 | APPARATUS AND METHOD FOR DETECTING BIOMOLECULES - Provided are an apparatus and method for detecting biomolecules. The apparatus includes a FET having a substrate, a source electrode, a drain electrode, a channel region between the source and drain electrodes, and probe molecules fixed to the channel region, wherein the source and drain electrodes are separated on the substrate, a microfluid supplier selectively supplying one of a reference buffer solution of low ionic concentration and a reaction solution of high ionic concentration containing target molecules, to the channel region of the FET to which the probe molecules are fixed, and a biomolecule detector detecting the target molecules by measuring a first current value of the channel region of the FET, and a second current value of the channel region of the FET to which the target molecules and the probe molecules that bind to each other in the reaction solution of high ionic concentration are fixed. | 07-07-2011 |
20110165558 | METHOD FOR IDENTIFYING INDIVIDUAL VIRUSES IN A SAMPLE - Individual viruses in any type of sample are identified quickly, unambiguously and reliably, and with the least possible preparation-related and technology-related expenditure, without necessitating immobilization using antibodies and without requiring an indication or at least a suspicion of potentially present viruses. This is accomplished by scanning the height profile of the sample, from which scanning sites suspected of containing viruses are selected, exposing those cites to monochromatic excitation light and spectroscopically analyzing the resulting Raman scattered light. | 07-07-2011 |
20110171627 | ZCYTOR19 HETERODIMERIC CYTOKINE RECEPTOR POLYNUCLEOTIDES, POLYPEPTIDES, ANTIBODIES AND METHODS - Novel methods are disclosed for forming a heterodimeric receptor complex with IL-28R and CRF2-4. The methods may be used for detecting and treating viral infections in in vitro and in vivo. Ligand-binding receptor polypeptides can also be used to block ligand activity in vitro and in vivo. The present invention also includes methods for producing the protein, uses therefor and antibodies thereto. | 07-14-2011 |
20110171628 | CERVICAL SCREENING ALGORITHMS - This invention describes new protocols for screening for cervical carcinomas or high-grade premalignant cervical lesions based on combinations of testing for the presence of high-risk HPV, HPV genotyping, marker analysis, and/or cytology. With these protocols the number of women that have to undergo follow-updiagnostic testing and/or clinical examinations will decrease. Further, the number of false positives and false negatives will decrease. | 07-14-2011 |
20110171629 | NANOSTRUCTURED DEVICES INCLUDING ANALYTE DETECTORS, AND RELATED METHODS - The present invention provides compositions and devices comprising nanostructure networks, and related methods. The compositions may exhibit enhanced interaction between nanostructures, providing improved device performance (e.g., improved conductivity). In some embodiments, the devices are capable of interacting with various species to produce an observable signal from the device. In some cases, the compositions and devices may be useful in the determination of analytes, including—biological analytes (e.g., DNA, ebola virus, other infective agents, etc.), small, organic analytes, and the like. The embodiments described herein may exhibit high sensitivity and specificity to analytes and may be capable of analyte detection at femtomolar concentrations (e.g., 10 fM). | 07-14-2011 |
20110177492 | METHOD OF CLASSIFYING CHEMICALLY CROSSLINKED CELLULAR SAMPLES USING MASS SPECTRA - A method of analyzing cellular samples that include a chemically crosslinked analyte is provided. The analysis typically involves the use of mass spectrometry. | 07-21-2011 |
20110177493 | USING HIGHLY SENSITIVE SUSPENDED CARBON NANOTUBES FOR MOLECULAR-LEVEL SENSING BASED ON OPTICAL DETECTION - A molecular sensor is provided that contains at least one carbon nanotube suspended on a suitable support structure. In one aspect, at least one receptor is attached to a surface of the suspended carbon nanotube. Also provided are methods of detecting an analyte in a sample by contacting a sample suspected of containing the analyte with the molecular sensor of this invention under suitable conditions that favor binding of the analyte to the receptor and detecting any analyte bound to the receptor, if present. | 07-21-2011 |
20110177494 | DEVICE AND METHOD FOR PRESSURE-DRIVEN PLUG TRANSPORT - The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid. | 07-21-2011 |
20110177495 | CONTINUOUS DIRECTED EVOLUTION OF PROTEINS AND NUCLEIC ACIDS - The present invention discloses generalizable methods of evolving nucleic acids and proteins utilizing continuous directed evolution. The invention discloses methods of passing a nucleic acid from cell to cell in a desired function-dependent manner. The linkage of the desired function and passage of the nucleic acid from cell to cell allows for continuous selection and mutation of the nucleic acid. | 07-21-2011 |
20110183313 | Genome Wide Visual Identification of Human Co-Factors of HIV-1 Infection - The present invention relates to the identification of human host factors involved in the early stage of HIV infection. Furthermore, it relates to the use of the identified genes for the elucidation of the mechanism of HIV-infection, as drug targets, and for identifying a compound useful in the treatment of HIV. | 07-28-2011 |
20110183314 | BACTERIOPHAGE-BASED MICROORGANISM DIAGNOSTIC ASSAY USING SPEED OR ACCELERATION OF BACTERIOPHAGE REPRODUCTION - A method of determining the presence or absence of a target microorganism in a sample to be tested, the method comprising: combining with the sample an amount of bacteriophage capable of infecting the target microorganism to create a bacteriophage-exposed sample; and measuring the time rate of change of the amount of said bacteriophage or the change in the rate of change of the amount of said bacteriophage as an indication of the presence or absence of the target microorganism as a function of time. | 07-28-2011 |
20110183315 | DETECTION OF WEST NILE VIRUS NUCLEIC ACIDS IN THE VIRAL 3' NON-CODING REGION - Methods for detecting flavivirus nucleic acids. Particularly described are methods for detecting West Nile virus nucleic acids in the 3′ non-coding region. | 07-28-2011 |
20110183316 | METHODS FOR DETECTING ORGANISMS AND ENZYMATIC REACTIONS USING RAMAN SPECTROSCOPY - The present disclosure provides systems for the rapid and sensitive detection of organisms and molecules in samples. Reactants that produce Raman-active products are used in combination with Raman light scattering. The present disclosure can also be used to measure enzyme-kinetics. | 07-28-2011 |
20110183317 | DETERMINING THE SENSITIVITY OF A CELL TO A DRUG - The present invention provides an in vitro method for determining the resistance or sensitivity of a cell line or patient sample to a deoxyribonucleoside kinase-dependent drug, wherein the method comprises the steps of: (i) treating a patient sample or cell line, or a portion thereof, with a deoxyribonucleoside kinase-dependent drug; (ii) lysing the cells of the patient sample or cell line from step (i); (iii) optionally, mixing a portion of the cell lysate from step (ii) with a bioluminescent reporter bacteria incorporating a gene coding for deoxyribonucleoside kinase; (iv) mixing a portion of the cell lysate from (ii) with a bioluminescent reporter bacteria incorporating a gene coding for a deoxyribonucleoside kinase and a deoxyribonucleoside kinase transcription promoter; (v) mixing a portion of the cell lysate from step (ii) with a bioluminescent reporter bacteria incorporating a gene coding for a deoxyribonucleoside kinase, a deoxyribonucleoside kinase transcription promoter and a dephosphorylating agent; and (vi) measuring the bioluminescence of each of the mixtures from steps (iii) to (v), wherein the comparative levels of bioluminescence of each of the mixtures provides a measure of the resistance or sensitivity to the drug. | 07-28-2011 |
20110189651 | Method and Device for Detecting Feline Immunodeficiency Virus - A method and device for determining a feline immunodeficiency virus infection or vaccination in an animal. The method includes contacting a biological sample from a felid with various FIV polypeptides and determining the binding of antibodies in the sample to the polypeptides. The determination of whether an animal is infected with FIV or has been vaccinated against FIV can be determined by measuring the animal's immune response to an FIV env polypeptide. A device for detecting FIV antibodies is provided. | 08-04-2011 |
20110189652 | VIRAL VARIANTS WITH ALTERED SUSCEPTABILITY TO NUCLOESIDE ANALOGS AND USES THEREOF - The present invention relates generally to viral variants exhibiting reduced sensitivity to particular agents and/or reduced interactivity with immunological reagents. More particularly, the present invention is directed to hepatitis B virus (HBV) variants exhibiting complete or partial resistance to nucleoside analogs and/or reduced interactivity with antibodies to viral surface components including reduced sensitivity to these antibodies. The present invention further contemplates assays for detecting such viral variants, which assays are useful in monitoring anti-viral therapeutic regimens and in developing new or modified vaccines directed against viral agents and in particular HBV variants. The present invention also contemplates the use of the viral variants to screen for agents capable of inhibiting infection, replication and/or release of the virus. | 08-04-2011 |
20110189653 | DETECTION AND PROGNOSIS OF CERVICAL CANCER - The present invention relates to methods and kits for identifying, diagnosing, prognosing, and monitoring cervical cancer. These methods include determining the methylation status or the expression levels of particular genes, or a combination thereof. | 08-04-2011 |
20110189654 | DIAGNOSTIC REAGENT, CONTAINING BIOPARTICLES, METHOD FOR PRODUCTION THEREOF AND USE THEREOF AS INTERNAL STANDARD IN NUCLEIC ACID PREPARATION AND NUCLEIC ACID DETECTION METHODS - A diagnostic reagent in the form of a composition dimensionally stable under standard conditions, comprising bioparticles and also customary pharmaceutical excipients, wherein the bioparticles are selected from the group consisting of bacteria, viruses, fungi, protozoa, bacteriophages, yeasts, spores, parasites, plant cells, animal or human cells, gametes, plasmids, and viroids. | 08-04-2011 |
20110189655 | REAGENTS AND KITS FOR DETECTION OF INFLUENZA VIRUS AND THE LIKE - The present invention relates to reagents and methods for influenza virus detection. These reagents and methods disclosed in the present invention enable simple, rapid, specific and sensitive detection of influenza virus types A and B. These reagents are N-acetylneuraminic acid-firefly luciferin conjugates which can be cleaved by influenza virus neuraminidase. | 08-04-2011 |
20110189656 | Method for Determining Presence or Absence of Abnormal Cell - A method for determining the presence or absence of an abnormal cell in a sample collected from the uterine cervix of a subject, and a method for predicting the progression of a lesion in the uterine cervix in a subject, each of which comprises measuring the frequency of methylation in the genomic DNA of human papillomavirus contained in the sample and determining the presence or absence of the abnormal cell or predicting the progression of the lesion based on the frequency; and a primer set which can be used in the above-mentioned methods. | 08-04-2011 |
20110195394 | SYSTEMS AND METHODS FOR DIAGNOSING VIRAL INFECTIONS - Systems and methods for diagnosing viral infections. According to at least one embodiment of a stabilizing system of the present disclosure, the system comprises a stabilizing agent useful to completely or substantially prevent degradation or inactivation of a diagnostic marker for a viral infection in a body fluid comprising the diagnostic marker and a detection agent capable of detecting the diagnostic marker | 08-11-2011 |
20110200982 | GENETICALLY MODIFIED MICE AND ENGRAFTMENT - A mouse with a humanization of the mIL-3 gene and the mGM-CSF gene, a knockout of a mRAG gene, and a knockout of a mIl2rg subunit gene; and optionally a humanization of the TPO gene is described. A RAG/Il2rg KO/hTPO knock-in mouse is described. A mouse engrafted with human hematopoietic stem cells (HSCs) that maintains a human immune cell (HIC) population derived from the HSCs and that is infectable by a human pathogen, e.g., | 08-18-2011 |
20110200983 | METHODS AND MATERIALS FOR DETECTING VIRAL OR MICROBIAL INFECTIONS - This document provides methods and materials for detecting target nucleic acid. For example, methods and materials for detecting the presence or absence of target nucleic acid, methods and materials for detecting the amount of target nucleic acid present within a sample, kits for detecting the presence or absence of target nucleic acid, kits for detecting the amount of target nucleic acid present within a sample, and methods for making such kits are provided. | 08-18-2011 |
20110200984 | USING NUCLEIC ACIDS FOR CLINICAL MICROBIOLOGY TESTING - A process for analysing a biological sample, comprising the steps of: (a) identifying a micro-organism present within the sample; and (b) determining the effect of one or more antimicrobial(s) on a micro-organism from the sample, wherein steps (a) and (b) are performed by analysing the micro-organism's nucleic acid. Steps (a) and (b) will generally occur in that order, but they may take place concurrently. The steps may advantageously be performed within a single apparatus. Conveniently, the nucleic acid analyte used for step (a) is the same as that used in step (b) e.g. the same PCR amplicon. A micro-organism's nucleic acids can thus be used both to identify the presence of the micro-organism within a sample and then to assess the effect of antimicrobials on its growth. | 08-18-2011 |
20110200985 | COMPOSITIONS FOR USE IN IDENTIFICATION OF HERPESVIRUSES - The present invention relates generally to identification of herpesviruses and provides methods, compositions and kits useful for this purpose when combined, for example, with molecular mass or base composition analysis. | 08-18-2011 |
20110200986 | BIO-ASSAY USING LIQUID CRYSTALS - There is provided a method, an in vitro method and a detection system for detecting the presence of at least one biological molecule in at least one sample. The method can include, for example, providing a sample and/or a binding agent to a contact portion of a surface by way of at least one microfluidic channel; disposing a liquid crystal at the contact portion; determining whether the orientation of the liquid crystal changes after the sample contacts the binding agent, indicating the presence of the biological molecule; and determining length of bright region of the liquid crystal and/or change in interference color of said liquid crystal, and consequently indicating the quantity of said biological molecule. Also disclosed are methods of detecting biological molecules using at least one 4′-pentyl-biphenyl-4-R, where R may be at least one functional group selected from carboxylic acid, amine, aldehyde, and oligopeptide. | 08-18-2011 |
20110207115 | METHODS AND MATERIALS FOR DETECTING CONTAMINATED FOOD PRODUCTS - This document provides methods and materials for detecting contaminated food products. For example, methods and materials for using an enzymatic amplification cascade of restriction endonucleases to detect nucleic acid of a microorganism or virus (e.g., a pathogen) within a sample (e.g., food product sample) being tested, thereby assessing a food product for possible contamination are provided. | 08-25-2011 |
20110212434 | NOVEL HIV-BASED RECOMBINANT VIRAL CLONES AND USE THEREOF IN ANALYTICAL METHODS - The present invention refers to HIV-based recombinant viral clones that possess the general structure represented in FIG. | 09-01-2011 |
20110212435 | Antimicrobial Compounds - The compounds disclosed herein are isoxazole derivatives that are useful as antimicrobial compounds, particularly as anti-bacterial compounds. The disclosed methods comprise incubating at least two different substrates in the presence of at least one oxygenase to provide the disclosed compounds, or to prepare and identify compounds that have antimicrobial activity. | 09-01-2011 |
20110217693 | Generation of Replication Competent Viruses for Therapeutic Use - The present invention relates to the generation of replication-competent viruses having therapeutic utility. The replication-competent viruses of the invention can express proteins useful in the treatment of disease. | 09-08-2011 |
20110217694 | FLOW CYTOMETRY-BASED SYSTEMS AND METHODS FOR DETECTING MICROBES - In various embodiments, the present disclosure describes methods and systems for detecting microbes in a sample. The methods are generally applicable to quantifying the number of target bacteria in a sample counted from a detection region of a flow cytometer histogram. The detection methods can be employed in the presence of other microorganisms and other non-target microbe components to selectively quantify the amount of a target microbe. The methods are advantageous over those presently existing for testing of foodstuffs and diagnostic evaluation in their speed, accuracy and ease of use. Various swab collection devices and kits useful for practicing the present disclosure are also described herein. | 09-08-2011 |
20110217695 | BETAG1-IgG INTRON FOR ENHANCED ANTI-IGF1R EXPRESSON - The present invention provides polynucleotides for enhanced expression of a target gene such as an immunoglobulin. Methods of expressing a target gene using the polynucleotides of the invention are also covered. | 09-08-2011 |
20110223584 | Oligonucleotides For Detecting Human Papilloma Virus In A Test Sample - Oligonucleotides targeted to HPV Type 16 and/or Type 18 nucleic acid sequences which are particularly useful to aid in detecting HPV type 16 and or 18 are described. The oligonucleotides can aid in detecting HPV Type 16 and/or Type 18 in different ways such as by acting as hybridization assay probes, helper probes, and/or amplification primers. | 09-15-2011 |
20110223585 | ASSAY FOR LOCALIZED DETECTION OF ANALYTES - The present invention relates to a method for detecting an analyte in a sample, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, wherein said probes each comprise an analyte-binding moiety and can simultaneously bind to the analyte, and wherein (i) said first proximity probe comprises a nucleic acid moiety attached at one end to the analyte-binding moiety, wherein a circular or circularizable oligonucleotide is hybridized to said nucleic acid moiety before, during or after said contacting step; and (ii) said second proximity probe comprises an enzyme moiety, attached to the analyte-binding moiety, capable of directly or indirectly enabling rolling circle amplification (RCA) of the circular or, when it is circularized, of the circularizable oligonucleotide hybridized to the nucleic acid moiety of the first proximity probe, wherein said RCA is primed by said nucleic acid moiety of said first proximity probe; (b) if necessary, circularizing said oligonucleotide, to produce a circularized template for RCA; (c) subjecting said circular or circularized template to RCA, wherein if the enzyme moiety of the second proximity probe in step (a)(ii) is a DNA polymerase, this step does not utilize a free DNA polymerase; and (d) detecting a product of said RCA. | 09-15-2011 |
20110229874 | COMPOSITIONS AND METHODS USEFUL FOR HCV INFECTION - The present invention provides compositions comprising cells that can effectively produce HCV after HCV infection, compositions for culturing the cells, methods for making the composition and methods for infecting the cells in the composition with HCV. The present invention also provides methods for assaying HCV production and methods for evaluating compounds that affect the production of HCV. | 09-22-2011 |
20110229875 | METHOD OF MEASURING HUMAN CYP3A INDUCIBILITY - A method for measuring human CYP3A inducibility upon administration of a test drug, characterized in that anon-human animal to which a test drug is administered or a population of human cells cultured in a medium containing a test drug is infected with viruses (A) and (B); virus (A) being an adenovirus which is used as a vector and engineered by incorporating thereto a detectable reporter gene and at least 3 human PXR binding regions falling within an untranslated region of a human CYP3A gene, and virus (B) being an adenovirus which is used as a vector and engineered by incorporating thereto a human PXR cDNA; and subsequently expression level of the reporter gene is determined in the non-human animal or the cultured human cells. | 09-22-2011 |
20110229876 | BIOMARKERS FOR THE DETECTION OF HEAD AND NECK TUMORS - A method of detecting the presence of specific human papilloma virus and host cell biomarkers associated with head and neck tumors in biological samples, like saliva, blood or biopsy tissue, obtained from a subject. | 09-22-2011 |
20110236879 | METHODS AND COMPOSITIONS FOR ANALYTE DETECTION - The present invention is directed to methods and apparatus for detection of one or more analytes. Analytes include agents or components of infectious agents such as pathogenic virus, as well as enzymes, proteins and biomarkers. | 09-29-2011 |
20110236880 | Potentiated Biocidal Compositions and Methods of Use - The present technology relates to biocidal compositions and methods that contain and utilize at least one biocidal agent and at least one potentiator system wherein the resultant combination has an enhanced biocidal efficacy. The present technology also discloses a rapid screening assay for determining biocidal compositions with enhanced efficacy, e.g., a microbial contact kill time of 5 minutes or less. Further, the present technology provides a method of determining biocidally effective concentrations of biocidal compositions comprising at least one biocidal agent and at least one potentiator system. | 09-29-2011 |
20110236881 | MODULATION OF INFLUENZA VIRUS - The present invention provides, among other things, methods for the identification of compounds that are capable of modulating the activity of the influenza A virus. For example, the present methods provide platforms for identifying small molecule inhibitors that target the proton transport pathway defined at least in part by two or more of the highly conserved channel residues 27, 30, 31, 34, 37, 41, 44, and 45 of the influenza A M2 protein. In one aspect, the present invention is directed to methods comprising comparing spatial models of a plurality of test compounds with the spatial model of the pathway defined by at least two residues from among residues 27, 30, 31, 34, 37 or 41, 44, and 45 on one or more subunits of the M2 transmembrane protein of the influenza A virus to determine the spatial complementarity of each of the test compounds with the pathway; assessing the ability of the test compounds to bind to the pathway; and, based on the assessed ability of the test compounds to bind the pathway, determining the compound that modulates the activity of influenza A. | 09-29-2011 |
20110236882 | QUANTITATIVE MEASUREMENT OF NANO/MICRO PARTICLE ENDOCYTOSIS WITH CELL MASS SPECTROMETRY - Methods for detecting the presence of nanoparticles or microparticles by cell mass spectrometry (CMS) are provided. CMS methods are provided for determining the number of nanoparticles or microparticles in each cell. Nanoparticles whose intracellular concentration can be determines by the CMS methods of the invention include polymeric nanoparticles (NPs), liposomes, viral-based NPs, carbon nanotubes, diamond NPs, polymeric micelles, nanocarriers, liposomes, and viral nanoparticles. Determination of the efficiency of drug delivery and intracellular titer of pathogens according to the invention is disclosed. Methods for determining intracellular uptake of virus particles are provided. | 09-29-2011 |
20110236883 | DETECTION OF HERPES SIMPLEX VIRUS TYPES 1 AND 2 BY NUCLEIC ACID AMPLIFICATION - The present invention relates to a method of detecting the presence or absence of herpes simplex virus (HSV) in a sample based on amplifying a portion of the Glycoprotein G(US4) gene of HSV and detecting the presence of the amplified nucleic acid using primers and detector primers as described herewith. The method of the invention further identifies the type of HSV, either HSV-1 or HSV-2, in a sample. Also encompassed by the invention is a kit comprising the primers and detector primers which may be used with the amplification method described herewith. | 09-29-2011 |
20110236884 | MICROBUBBLES FOR AFFINITY SEPARATION - The present invention relates to methods, compositions and kits for affinity isolation, affinity purification and affinity assay based on microbubbles coated with an affinity molecule. Particularly, the invention provides protein microbubbles coated with an affinity molecule. In addition, the invention provides glass microbubbles coated with an affinity molecule. Methods of using the microbubbles of the invention for isolating analytes and cells are specifically provided. | 09-29-2011 |
20110244445 | Mass spectroscopy analysis of mutant polypeptides in biological samples - The invention relates to a method for determining the presence of at least one distinct polypeptide in a biological sample comprising contacting the biological sample with a hydrolyzing agent, wherein the hydrolyzing agent is capable of hydrolyzing the distinct polypeptide in a sequence-specific manner such that at least one distinct peptide having a predetermined peptide measured accurate mass would result if the at least one distinct polypeptide were present in the biological sample, to obtain a hydrolyzed sample; bringing the hydrolyzed sample in contact with a substrate comprising at least one immobilized binding partner, wherein the at least one immobilized binding partner is capable of specifically binding the distinct peptide; removing the hydrolyzed sample from the substrate in a manner such that the distinct peptide would remain bound to the immobilized binding partner; contacting the substrate with an elution solution, wherein the distinct peptide would dissociate from the immobilized binding partner into the elution solution; subjecting a portion of the elution solution to liquid chromatography to segregate a plurality of molecules in the portion of the elution solution to obtain sorted molecules; determining the measured accurate mass of at least one sorted molecule present in the elution solution; and determining the presence of the at least one distinct polypeptide in the biological sample when a measured accurate mass of at least one molecule is substantially equal to the predetermined peptide measured accurate mass. | 10-06-2011 |
20110250583 | OPTIMIZED PROBES AND PRIMERS AND METHODS OF USING SAME FOR THE BINDING, DETECTION, DIFFERENTIATION, ISOLATION AND SEQUENCING OF INFLUENZA A; INFLUENZA B; NOVEL INFLUENZA A/H1N1; AND A NOVEL INFLUENZA A/H1N1 RNA SEQUENCE MUTATION ASSOCIATED WITH OSELTAMIVIR RESISTANCE - Described herein are primers and probes useful for the binding, detecting, differentiating, isolating, and sequencing of influenza A, influenza B, 2009 influenza A/H1N1, and a 2009 influenza A/H1N1 RNA sequence mutation associated with oseltamivir resistance. | 10-13-2011 |
20110250584 | Method for Screening Cancer Therapeutic Agent Using Galectin-3, GSK-3Betta, and Fascin-1 - Disclosed is a method for screening a novel cancer therapeutic agent. The cancer therapeutic agent exhibits down-regulation of galectin-3 and fascin-1 or interferes with the interaction between galectin-3 and GSK-3β. | 10-13-2011 |
20110250585 | ORGAN MIMIC DEVICE WITH MICROCHANNELS AND METHODS OF USE AND MANUFACTURING THEREOF - System and method includes a body having a central microchannel separated by one or more porous membranes. The membranes are configured to divide the central microchannel into a two or more parallel central microchannels, wherein one or more first fluids are applied through the first central microchannel and one or more second fluids are applied through the second or more central microchannels. The surfaces of each porous membrane can be coated with cell adhesive molecules to support the attachment of cells and promote their organization into tissues on the upper and lower surface of the membrane. The pores may be large enough to only permit exchange of gases and small chemicals, or to permit migration and transchannel passage of large proteins and whole living cells. Fluid pressure, flow and channel geometry also may be varied to apply a desired mechanical force to one or both tissue layers. | 10-13-2011 |
20110250586 | SYSTEM AND METHOD FOR CONCENTRATING SAMPLES - A system and method for concentrating samples. The system can include a first container adapted to contain a sample. The first container can include a first portion and a second portion adapted to be removably coupled to the first portion. The system can further include a second container comprising the second portion and a third portion adapted to be removably coupled to the second portion. The method can include centrifuging the first container in a first orientation toward the second portion of the first container; retaining a concentrate of the sample in the second portion of the first container; and centrifuging the second container in a second orientation toward the third portion of the second container, such that the concentrate retained in the second portion is moved into the third portion of the second container, the second orientation being different from the first orientation. | 10-13-2011 |
20110250587 | NOVEL METHOD FOR GENERATION OF RNA VIRUS - The present invention provides a method for generating negative-stranded segmented RNA viruses using linear expression constructs in the presence of helper virus. | 10-13-2011 |
20110250588 | METHOD FOR DETECTING CANCER CELLS IN BLOOD SAMPLE - A method for detecting cancer cells in a blood sample is provided comprising the steps of proliferating an oncolytic virus at least in the cancer cells by incubating the blood sample being suspected to contain the cancer cells with the oncolytic virus; mixing the blood sample obtained from the proliferating step with a fixing agent and a nonionic surfactant; and detecting the cancer cells in the blood sample obtained from the mixing step, in which the oncolytic virus has been proliferated. | 10-13-2011 |
20110256524 | RECOMBINANT ADENOVIRUS COMPRISING TISSUE-SPECIFIC PROMOTER AND TUMOR-TARGETING TRANS-SPLICING RIBOZYME AND USES THEREOF - Disclosed herein are a recombinant adenovirus comprising tissue-specific promoters and trans-splicing ribozymes targeting tumor-specific genes, and uses thereof. More specifically, disclosed herein are a recombinant adenovirus comprising (1) a tissue-specific promoter, (2) a trans-splicing ribozyme acting on tumor-specific genes operably linked to the promoter, and (3) a therapeutic or reporter gene linked to 3′ exon of the ribozyme, an anticancer pharmaceutical composition comprising the same, and a composition for cancer diagnosis comprising the same. | 10-20-2011 |
20110256525 | COMPOSITIONS AND METHODS FOR PURIFYING AND CRYSTALLIZING MOLECULES OF INTEREST - A composition-of-matter is provided. The composition comprising at least one antibody binding moiety capable of binding an antibody-labeled target molecule, cell or virus of interest, said at least one antibody binding moiety being attached to at least one coordinating moiety selected capable of directing the composition-of-matter to form a non-covalent complex when co-incubated with a coordinator ion or molecule. | 10-20-2011 |
20110256526 | DETECTION OF HUMAN SOMATIC CELL REPROGRAMMING - The methods and kits described herein are based, in part, to the discovery phenotype representing a fully-reprogrammed iPS cell and several reprogramming intermediates. The methods and kits described herein permit identification of fully-reprogrammed iPS cells and further permits one of skill in the art to monitor the emergence of iPS cells during the reprogramming process. The methods/kits can also be performed using real time using live cell imaging. Also described herein are methods for screening candidate reprogramming agents by monitoring the emergence of fully-reprogrammed iPS cells in the presence and absence of such an agent. | 10-20-2011 |
20110256527 | RAPID AND SAFE TECHNIQUE FOR PERFORMING PCR AMPLIFICATIONS - This invention relates to methods for quick and safe identification of pathogens from biological samples. Iodinated resins may be employed to destroy a pathogen while leaving the pathogen's DNA in a state that can be analyzed. The DNA can then serve as a substrate for PCR analysis. The use of these iodinated resins work in a significantly quicker manner than prior art methods and allows scientists to spend a minimal time under Biosafety Level Three (BSL-3) conditions. | 10-20-2011 |
20110256528 | ANALYTE DETECTION ASSAY - A rapid and sensitive analyte detection assay is based on whispering gallery modes of fluorescently labelled microspheroidal particles. Ligands for the analyte, such as nucleic acids, are anchored to the particles. The fluorescent labels may comprise fluorophores or quantum dots. In the latter case, the particles may comprise melamine formaldehyde. The assay may be used to detect analytes in aqueous samples. | 10-20-2011 |
20110262892 | METHODS FOR DETECTION OR MEASUREMENT OF VIRUSES - A method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) an anionic surfactant and (2) an amphoteric surfactant, nonionic surfactant or protein denaturant; a virus assay method using said treating method; a method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) a chaotropic ion and (2) an acidifying agent; a virus assay method using said treating method; a virus assay method, characterized in that a virus antigen and a virus antibody are measured based on their binding to their probe in the presence of a surfactant with an alkyl group of 10 or more carbon atoms and a secondary, tertiary or quaternary amine, or a nonionic surfactant, or of both of them; and a monoclonal antibody and a hybridoma producing the same for carrying out said method. | 10-27-2011 |
20110262893 | SEPARATING TARGET ANALYTES USING ALTERNATING MAGNETIC FIELDS - The invention generally relates to using magnetic particles and alternating magnet fields to separate a target analyte from a sample. In certain embodiments, methods of the invention involve contacting a sample with magnetic particles including first moieties specific for a target analyte, thereby forming target/particle complexes in the sample, flowing the sample through a channel including second moieties attached to at least one surface of the channel, applying alternating magnetic fields to the flowing sample to result in target/particle complexes being brought into proximity of the surface to bind the second moieties and unbound particles remaining free in the sample, binding the target/particle complexes to the second moieties, and washing away unbound particles and unbound analytes of the sample. | 10-27-2011 |
20110262894 | VACCINE AGAINST INFECTIOUS AGENTS HAVING AN INTRACELLULAR PHASE, COMPOSITION FOR THE TREATMENT AND PREVENTION OF HIV INFECTIONS, ANTIBODIES AND METHODS OF DIAGNOSIS - A vaccine for treating and/or preventing infectious diseases where the infectious agent has at least one intracellular phase in the host during its multiplication cycle, is disclosed. The vaccine comprises at least one cryptic epitope of a cellular element that is carried along by an intracellular infectious agent as it leaves the cell, and revealed by said infectious agent. A composition for treating and/or preventing HIV infections, antibodies to a peptide of interest, and a diagnostic method, are also disclosed. | 10-27-2011 |
20110262895 | METHODS FOR THE DIAGNOSIS OF VARICELLA ZOSTER VIRUS INFECTION - The present invention relates to methods and devices for the rapid assessment of saliva for the presence of varicella zoster virus (VZV) particles. The methods and devices permit rapid, simple and cost-effective diagnosis of primary VZV infection. | 10-27-2011 |
20110262896 | SYSTEM AND METHOD FOR MULTI-ANALYTE DETECTION - The present invention provides a system and method for the simultaneous detection of multiple analytes in a sample. The detection system includes a housing that holds a reagent carousel rotatably coupled thereto. Further included in the housing is an incubator carousel rotatably coupled thereto. The housing also includes magnetic material that is associated with the incubation carousel for assisting in separation beads from reagent and wash solution. A robot, associated with the housing is configured to manipulate at least either the reagent carousel or the incubator carousel and transfer materials therebetween. Reaction vessels hold samples and reaction vessels handlers move the reaction vessels. Sample analysis is determined by at least one laser based detector. | 10-27-2011 |
20110262897 | IMAGING TECHNIQUES USING A TRIDENTATE LIGAND - The present invention relates to microscopy and, in particular, Time-resolved Emission Imaging Microscopy (TREM). The Invention relates to the use of a transition metal complex having a tridentate ligand in an imaging technique. The transition metal is preferably platinum. | 10-27-2011 |
20110269115 | Heated Assays for Influenza - Methods of increasing specific binding, decreasing non-specific binding, and reducing false-positive interaction in solid phase assays for influenza are disclosed. In the methods, the solid phase apparatus (lateral flow solid phase apparatus or capillary flow solid phase apparatus) is subjected to elevated heat subsequent to application of a test sample to the solid phase apparatus. | 11-03-2011 |
20110269116 | Cell Line From Rousettus As Host Cell For Pathogen Amplification - The present invention relates to permanent cell lines from chiropterans suitable for amplification and production microbial agents, preferably viruses, and its use for diagnostic or therapeutic purposes. | 11-03-2011 |
20110269117 | Methods For Direct Fluorescent Antibody Virus Detection In Liquids - The present invention describes a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The assay includes centrifugation of the specimen, incubation of sample and reagents in solution, and detection of the absence or presence of respiratory virus. Sapogenin is used as a detergent to permeabilize the cells for entry of the monoclonal antibodies to react with intracellular antigens. The cells are stained with fluorescently labeled monoclonal antibodies against the viral antigens along with a background stain and a fluorescent nuclear stain. This counter staining decreases background and allows co-localization of antigen and nuclear structures for enhanced detection. | 11-03-2011 |
20110269118 | METHODS FOR IDENTIFYING CELLS BY COMBINATORIAL FLUORESCENCE IMAGING - A method of identifying the taxonomic or functional classification of cells in situ involves labeling the cells with a set of nucleic acid probes and performing combinatorial fluorescence microscopic imaging. The set of probes contains groups of either two or three probes that bind to a taxon-specific or function-specific nucleotide sequence. Each probe of a group of probes is labeled with a distinct fluorescent label, and each group corresponds to a unique combination of labels, which can be detected across the image and serves to identify cells having a single target sequence, or a set of target sequences, that are characteristic of a unique taxonomic or functional classification. The combinatorial labeling and spectral imaging approach greatly expands the number of different classifications that can be identified simultaneously in a single image of a collection of cells. The methods and probe sets of the invention can be used to rapidly identify microbes, study their ecological relationships, screen for novel antibiotics, and identify pathogens. | 11-03-2011 |
20110275058 | SELF-CONTAINED BIOLOGICAL ASSAY APPARATUS, METHODS, AND APPLICATIONS - A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing. | 11-10-2011 |
20110275059 | Diagnostic Transcript and Splice Patterns of HR-HPV in Different Cervical Lesions - The present invention relates to a method for differentiating in a subject with HR-HPV between a severe form of HR-HPV infection and a mild form of HR-HPV infection. It further is concerned with a composition comprising a probe oligonucleotide mixture, a device, and a kit for use in conjunction with the method of the invention. | 11-10-2011 |
20110275060 | DIAGNOSING AND MONITORING INFLAMMATORY DISEASES BY MEASURING COMPLEMENT COMPONENTS ON WHITE BLOOD CELLS - The invention is related to methods of diagnosing inflammatory diseases or conditions by determining levels of components of the complement pathway on the surface of white blood cells. | 11-10-2011 |
20110281258 | Human Immunodeficiency Virus And Uses Thereof - The present invention relates to Human Immunodeficiency Virus-1 (HIV-1) Group P of the strain designated 06CMU14788 and fragments thereof, primers which are derived from HIV-1 Group P, immunogenic regions thereof, immunoassays and nucleic acid based assays for the detection of Human Immunodeficiency Virus (HIV) that employ said HIV-1 Group P or fragments thereof and therapeutic compositions containing said HIV-1 Group P or fragments thereof. | 11-17-2011 |
20110287406 | Novel HCV core+1 protein, methods for diagnosis of HCV infections, prophylaxis, and for screening of anti-HCV agents - The present invention relates to a novel form of core+1 protein of Hepatitis C virus (HCV), designated shorter form core+1 protein. The shorter form core+1 protein of Hepatitis C virus is the product of translation of a coding sequence consisting of all or part of a nucleotide sequence extending from nucleotide 598 to nucleotide 920 within the core+1 ORF of HCV represented on FIG. | 11-24-2011 |
20110287407 | INTEGRATED METHODS AND SYSTEMS FOR PROCESSING A MOLECULAR PROFILE - An automated and integrated matching of a biological specimen to an individual, can include a collector having substrate materials and configured to selectively collect and release at least one bio-molecular species from a biological specimen; a self-contained mobile automated testing instrument configured to receive the biological specimen and further configured to generate, store and output a molecular profile of the at least one predetermined bio-molecular species; a processor based system communicatively coupled to the self-contained mobile automated testing instrument and configured to receive the molecular profile; and wherein the processor based system is configured to provide a quantitative comparative analysis and report of the molecular profile with a selected molecular profile. In one approach, at least one predetermined bio-molecular species and stored pre-selected molecular profile is for a predetermined sequence of interest, such as a DNA-profile as defined by a Combined DNA Index System (CODIS) system. | 11-24-2011 |
20110287408 | Preparation of Micro-Porous Crystals and Conjugates Thereof - A conjugate comprising a dye-labeled microporous crystal, a stop-cock moiety, and covalently bound thereto an affinity binding agent is disclosed. The dye-labeled microporous crystal is a zeolite crystal, such as a zeolite L crystal, having a large number of channels in its interior into which the dye is loaded. The stop-cock moiety can be functionalized with an amino group to which a carboxyester group can be attached. The affinity binding agent allows for the binding to a biological moiety. The conjugate of the moiety can be used in in-vivo and/or in-vitro imaging applications. | 11-24-2011 |
20110287409 | DIAGNOSTIC DEVICE AND METHOD - Diagnostic devices and methods involve comparison of relative levels of first and second components and/or characteristics of a fluid sample (e.g., saliva), preferably using bound antibodies arranged to interact with selected components, and colorimetric indicators that are released in proportion to relative concentration or amount of the components or characteristics, as indicative of a health condition such as dehydration state, shock state, stress state, disease state, drug consumption, and drug metabolization. Amylase and IgA may be selected as specific salivary components of interest. | 11-24-2011 |
20110287410 | HEPATITIS-C VIRUS TESTING - New styles of hepatitis C virus (HCV), referred to as HCV-3 and HCV-4, have been identified and sequenced. Antigenic regions of HCV-2, HCV-3 and HCV-4 polypeptides have been identified. Immunoassays for HCV and antibodies thereto are described, which allow more complete screening of blood samples for HCV, and allow HCV genotyping. | 11-24-2011 |
20110294109 | METHODS FOR DETECTING AN ANALYTE - Methods of decreasing non-specific binding in solid phase assays for an analyte are disclosed. In the methods, the solid phase apparatus (lateral flow solid phase apparatus or capillary flow solid phase apparatus) is subjected to elevated heat. The elevated heat can be applied subsequent to application of a test sample to the solid phase apparatus. | 12-01-2011 |
20110294110 | SET OF MAGNETIC LABELS - Provided is a label for an analyte, which label is attached to a magnetic or magnetisable substance, the label comprising:
| 12-01-2011 |
20110294111 | METHOD OF DETERMINING AND CONFIRMING THE PRESENCE OF AN HPV IN A SAMPLE - Methods are provided for genotyping a target nucleic acid in a sample. In various aspects, the methods comprise generating nucleic acid hybrids between probes specific for the genotypes of interest and the target nucleic acid and detecting hybridization in the sample. In other aspects, the methods comprise using multi-probe mixtures to reduce the volume of sample necessary to determine the genotype of the target nucleic acid. | 12-01-2011 |
20110294112 | METHODS FOR POINT-OF-CARE DETECTION OF NUCLEIC ACID IN A SAMPLE - Provided herein are methods and apparatus for detecting a target nucleic acid in a sample and related methods and apparatus for diagnosing a condition in an individual. The condition is associated with presence of nucleic acid produced by certain pathogens in the individual. | 12-01-2011 |
20110294113 | DETECTION DEVICE FOR DETECTING BIOLOGICAL MICROPARTICLES SUCH AS BACTERIA, VIRUSES, SPORES, POLLEN OR BIOLOGICAL TOXINS, AND DETECTION METHOD - A device for the detection of micro particles that can be marked by probes or antibodies capable of being detected by radiation has a filter, a supply system, and a detection system. Fluid to be examined is passed over a filter to filter out the micro particles and to perform the marking steps by supplying corresponding marking substances to the filter | 12-01-2011 |
20110300528 | Methods for Bacteriophage Design - Methods for designing and breeding phages are described. The methods include methods to design phages for previously resistant bacterial strains. The methods described do not use genetic manipulation techniques. | 12-08-2011 |
20110300529 | DETECTION OF IFI16 IN BODY FLUIDS - The present invention relates to methods for the qualitative and/or quantitative determination of interferon inducible protein 16 (IFI 16) in an extracellular form. | 12-08-2011 |
20110300530 | IN VITRO TEST SYSTEM FOR VIRAL INFECTIONS - The invention relates to a multi-layered biological in vitro tissue containing: dermis layer, containing a collagen biomatrix with fibroblasts embedded therein and epidermis layer, containing epithelial cells. It is provided that latently virally infected neuronal cells are integrated at least into the dermis layer. | 12-08-2011 |
20110300531 | METHOD AND DEVICE TO DETECT THE PRESENCE OF ANALYTES IN A SAMPLE - Disclosed are methods and apparatus useful for determining the presence or absence of one or more analytes in a liquid sample, such as a biological or environmental sample. In some embodiments, the method can use an indirect competitive immunochromatographic test strip. | 12-08-2011 |
20110306034 | METHOD FOR ISOLATING CELLS AND DISEASE VECTORS FROM BODILY FLUIDS - Embodiments provide one of a method, a device, and their use to isolate or analyze cells, including pathogens from body fluids by means of different separation methods. | 12-15-2011 |
20110306035 | Methods and Compositions for Detection of a Pathogen, Disease, Medical Condition, or Biomarker Thereof - Provided are methods for detecting the presence or absence of a pathogen, disease, or medical condition, or biomarker thereof, using an enzymatic activity assay. In one embodiment, the method provided utilizes competitive inhibition of an enzyme for detecting a pathogen, disease, or medical condition, or biomarker thereof, in a subject. The method comprises providing a biological sample from the subject that may or may not contain an endogenous substrate. A test reaction is provided by contacting the biological sample with an enzyme indicative of the biomarker of a pathogen, disease, or medical condition and a substrate comprising a signaling moiety. The enzyme modifies the endogenous substrate and the substrate comprising the signaling moiety. Modification of the substrate comprising the signaling moiety by the enzyme produces a signal from the signaling moiety. Data from a control reaction comprising the enzyme and the substrate comprising the signaling moiety is further provided. The signal produced by the signaling moiety in the test reaction is detected. The presence of the biomarker of the pathogen, disease, or medical condition is indicated by a difference caused by the presence of the endogenous substrate in the biological sample between the signal produced in the test reaction and the data from the control reaction. In another embodiment, there is provide a method of detecting the presence or absence of enzymatic activity in a biological sample indicative of a pathogen, disease, or medical condition, or biomarker thereof, in a subject. The method comprises contacting a biological sample obtained from a subject that may or may not contain an enzyme with a substrate of the enzyme to be detected. The substrate comprises a signaling molecule such that when the enzyme is present in the biological sample, the enzyme modifies the substrate and the signaling moiety emits a signal, indicating the presence of a pathogen, disease, or a medical condition, or a biomarker thereof, in the subject. | 12-15-2011 |
20110306036 | RT-LAMP assay for the detection of pan-serotype dengue virus - The invention relates to a reverse transcription loop-mediated isothermal amplification (LAMP) assay for the detection of dengue virus. The assay is capable of simultaneous detection of dengue 1-4 serotypes in a single reaction. | 12-15-2011 |
20110306037 | OLIGONUCLEOTUIDE PROBES AND PRIMERS FOR DETECTION OF HEPATITIS B VIRUS - The present disclosure provides a method for the detection and quantification of Hepatitis B Virus. It discloses oligonucleotide probes set forth in SEQ ID Nos. 1 and 2 for detection of Hepatitis B Virus along with respective primers [sense and antisense] set forth in SEQ ID Nos. 3, 4, 5 and 6. It also provides a PCR reaction mixture for detection of Hepatitis B Virus and a kit for detection of HBV comprising said mixture along with an instruction package. | 12-15-2011 |
20110306038 | ASSAY FOR DETECTION OF HUMAN PARVOVIRUS NUCLEIC ACID - Nucleic acid oligomers specific for human parvovirus genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus genotypes 1, 2 and 3 nucleic acid in biological specimens is disclosed. Compositions for amplifying and detecting the presence of human parvovirus genotypes 1, 2 and 3 genomic DNA in human biological specimens are disclosed. | 12-15-2011 |
20110311961 | DETECTION OF NUCLEIC ACIDS FROM MULTIPLE TYPES OF HUMAN PAPILLOMAVIRUSES - Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed. | 12-22-2011 |
20110311962 | METHOD FOR HIGH-RESOLUTION DETECTION OF NANOPARTICLES ON TWO-DIMENSIONAL DETECTOR SURFACES - The invention relates to a surface plasmon resonance spectrometer comprising a radiation source that emits substantially monochromatic radiation, a sensor surface, an optical arrangement for lighting the sensor surface by the radiation emitted from the radiation source such that surface plasmons can be created in the sensor surface, a detector having a plurality of image elements and observation optics for depicting the radiation reflected by the sensor surface on the detector, characterized in that the resolution capability of the observation optics and of the detector is larger than the resolution that can be obtained by the deflection-limited radiation source. | 12-22-2011 |
20110318727 | GENETIC MARKER FOR DETECTION OF HUMAN PAPILLOMAVIRUS - The invention provides compositions and methods for the differential detection of high risk forms of HPV from a urine sample provided by a patient. Specifically, the invention provides primers and probes that specifically recognize and bind sequences within the E1 gene of HPV. Detection of high risk forms of HPV identify individuals at risk of developing or in the early stages of cervical carcinoma. | 12-29-2011 |
20110318728 | SYSTEMS, DEVICES, METHODS AND KITS FOR FLUID HANDLING - Fluid handling devices, systems, methods and kits are disclosed. Fluid handling devices according to the disclosure comprise: an inlet for receiving a sample; a reagent layer comprising, a substrate having a first surface and an opposing second surface, at least one reagent storage compartment configured to hold a reagent, and a seal in communication with the at least one reagent storage compartment; and a reaction layer having a first surface and an opposing second surface comprising, a reaction area, and an outlet in communication with the reaction area, wherein the reagent layer and reaction layer are adapted and configured to permit movement of at least one of the reagent layer and the reaction layer in a plane relative to the other layer. | 12-29-2011 |
20110318729 | Rapid Bioluminescence Detection Assay - An assay is provided for detecting the activity of a reporter kinase comprising (i) adding said reporter kinase to an assay mixture wherein said reporter kinase is contacted with bioluminescent reagent no more than minutes after being contacted with ADP, and wherein, prior to contacting the reporter kinase with ADP, the assay mixture is substantially free from kinase other than reporter kinase; and (ii) detecting light output from the assay mixture. Methods for detecting the presence of an analyte in a sample and methods for validating a treatment process using the above assay are also provided. Further provided are devices for conducting these assays and methods. | 12-29-2011 |
20120003624 | STANDARDIZED METHOD AND KIT FOR THE QUANTIFICATION OF HEPATITIS A VIRUS - The present invention provides a standardized method and a kit for an accurate quantification of HAV in clinical and food samples. The general approach is based on the use of several controls to measure the efficiency of those critical steps of the quantification: the nucleic acids extraction and the RT-PCR reactions. The kit comprises: a Mengo virus mutant strain with the same growth properties than those of the wild-type Mengo virus and with no pathogenic capacity; a single stranded RNA molecule corresponding to a fragment of the HAV genome; primers that specifically bind to regions of the 5′ non coding region of the HAV genome; a detectable labeled probe that specifically binds to the amplimer resulting from the RT-PCR; and an appropriate molecule to generate an standard curve for the quantification of HAV. | 01-05-2012 |
20120003625 | METHODS AND KITS FOR DETECTING AN INFECTIOUS AGENT - The present invention provides methods and kits for determining the presence, absence, or level of an infectious agent in a sample. Specifically, the present invention provides methods and kits for detecting or quantifying certain target polynucleotides of the infectious agent. In certain embodiments, the present invention provides for such detection without the need for amplification (e.g., replication) of the target molecule and/or without the need for labor intensive purification procedures. In certain embodiments, the present invention provides positive control and housekeeping gene for normalization and quantatively detection of the copy numbers of infectious agent in a sample. In these or other embodiments, the invention allows for such detection with the desired sensitivity and/or specificity, even where the polynucleotide is present in the sample at low copy number. | 01-05-2012 |
20120003626 | SAMPLING DEVICES AND METHODS FOR CONCENTRATING MICROORGANISMS - The present disclosure describes methods for concentrating microorganisms with concentration agents in a sampling device and the sampling device described herein. More specifically, methods for concentrating microorganisms from large volume samples with concentration agents in a sampling device can provide for rapid, low cost, simple (involving no complex equipment or procedures), and/or effective processes under a variety of conditions. | 01-05-2012 |
20120003627 | Portable Fluorescence Reader Device - The present invention describes a device for performing a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The device also includes a compatible slide comprising sample wells. The device detects emitted fluorescence signal through a camera and optics assembly that is controlled by a user interface assembly. | 01-05-2012 |
20120003628 | Methods for Diagnosing Pervasive Development Disorders, Dysautonomia and Other Neurological Conditions - Methods for aiding in the diagnosis of disorders including, but not limited to, PDDs (Pervasive Development Disorders), Dysautonomic disorders, Parkinson's disease and SIDS (Sudden Infant Death Syndrome). In one aspect, a diagnosis method comprises analyzing a stool sample of an individual for the presence of a biological marker (or marker compound) comprising one or more pathogens, which provides an indication of whether the individual has, or can develop, a disorder including, but not limited to, a PDD, Dysautonomia, Parkinsons disease and SIDS. Preferably, the presence of one or more pathogens is determined using a stool immunoassay to determine the presence of antigens in a stool sample, wherein such antigens are associated with one or more pathogens including, but not limited to, | 01-05-2012 |
20120009560 | Method For Purifying Nucleic Acids From Microorganisms Present In Liquid Samples - The present invention relates to a method for treating liquid samples with a view to detecting any possible pathogenic microorganisms in a very small amount. More specifically, this method comprises a generic step of capturing and concentrating microorganisms on an ion exchange surface, followed by an in situ lysis treatment carried out on the microorganisms and capture of the nucleic acids released during the lysis. The implementation of this method enables an extremely concentrated and purified solution of nucleic acids to be obtained. This method is suitable for a continuous treatment of liquid samples. The invention also relates to a device for analysing liquid samples for biology, health or the environment, which is suitable for the implementation of the method according to the invention. | 01-12-2012 |
20120009561 | METHODS OF EVALUATING A TEST AGENT IN A DISEASED CELL MODEL - The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the tissues of the immune system in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates and other materials in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interactions with the immune system, coupled with disease models to provide a more complete representation of an immune response. | 01-12-2012 |
20120009562 | High-Risk Human Papillomavirus Detection - This invention provides compositions and methods for detecting HPV in a sample. This invention also provides related kits, systems, and computers. | 01-12-2012 |
20120009563 | High-Risk Human Papillomavirus Detection - This invention provides compositions and methods for detecting HPV in a sample. This invention also provides related kits, systems, and computers. | 01-12-2012 |
20120009564 | High-Risk Human Papillomavirus Detection - This invention provides compositions and methods for detecting HPV in a sample. This invention also provides related kits, systems, and computers. | 01-12-2012 |
20120009565 | COMPOSITIONS AND METHODS FOR DETECTION OF HEPATITIS A VIRUS NUCLEIC ACID - Nucleic acid oligomeric sequences and in vitro nucleic acid amplification and detection methods for detecting the presence of HAV RNA sequences in samples are disclosed. Kits comprising nucleic acid oligomers for amplifying and detecting HAV nucleic acid sequences are disclosed. | 01-12-2012 |
20120009566 | LUMINESCENCE ASSAY METHOD - A bioassay employing a first group including a lanthanide ion carrier chelate and a first recognition element, a second group including an antenna ligand and a second recognition element; where the lanthanide ion carrier chelate binds strongly to lanthanide, or the lanthanide ion carrier chelate binds moderately to lanthanide, and an agent complexing the lanthanide ion is additionally employed at a concentration of at least 1 pmol/l. The antenna ligand binds weakly to the lanthanide ion. Analyte recognition by the first recognition element and by the second recognition element results in either chelate complementation and increased fluorescence, or chelate discomplementation and decreased fluorescence. | 01-12-2012 |
20120015344 | Methods, compositions, and apparatus for the detection of viral strains - The disclosure generally relates to a particulate composition formed from a conductive polymer bound to magnetic nanoparticles. The particulate composition can be formed into a biologically enhanced, electrically active magnetic (BEAM) nanoparticle composition by further including a binding pair member (e.g., an antibody or a fragment thereof that specifically recognizes a virus strain or a virus surface protein) bound to the conductive polymer of the particulate composition. The disclosure further provides compositions, kits, detection apparatus, and methods for detecting specific viral strains including those with pandemic potential. In the various embodiments, a triplex including the BEAM nanoparticle, a virus or virally derived material (e.g. strain- and/or strain subtype specific viral surface protein or fragments thereof), and a viral strain subtype-specific binding pair member (e.g., a glycan that recognizes a specific virus strain subtype) is formed and detected, such as by use of a biosensor. | 01-19-2012 |
20120015345 | Identification of Oligonucleotides for the Capture, Detection and Quantitation of West Nile Virus - West Nile virus capture oligonucleotides, primers and probes derived from conserved regions of the West Nile virus genome are disclosed. Also disclosed are nucleic acid-based assays using the capture oligonucleotides, primers and probes. | 01-19-2012 |
20120015346 | INFLUENZA VIRUS DETECTION AND DIAGNOSIS - The invention discloses compositions comprising nucleic acid(s) for rapid detection, identification, differentiation, and/or diagnosis of certain Influenza virus A subtypes, e.g., H1N1, H3N2 and A(2009 H1N1)pdm, and methods of use thereof, e.g., Short-run RT-PCR, including an RT-PCR assay kit, comprising the disclosed composition(s). | 01-19-2012 |
20120015347 | Methods for Assaying Cellular Binding Interactions - There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule. | 01-19-2012 |
20120015348 | COMPOSITIONS AND METHODS FOR DETECTION OF HEPATITIS A VIRUS NUCLEIC ACID - Nucleic acid oligomeric sequences and in vitro nucleic acid amplification and detection methods for detecting the presence of HAV RNA sequences in samples are disclosed. Kits comprising nucleic acid oligomers for amplifying and detecting HAV nucleic acid sequences are disclosed. | 01-19-2012 |
20120015349 | COMPOSITIONS FOR USE IN IDENTIFICATION OF PAPILLOMAVIRUS - The present invention relates generally to identification of HPV, and provides methods, compositions and kits useful for this purpose when combined, for example, with molecular mass or base composition analysis. | 01-19-2012 |
20120015350 | LATERAL FLOW STRIP AND USES THEREOF - The present invention relates to lateral flow strip assay system and uses thereof. In particular, the present invention relates to lateral flow assay systems for the simple and inexpensive detection of biomolecules. | 01-19-2012 |
20120021402 | BIOSENSOR APPARATUSES AND METHODS THEREOF - A biosensor has one or more field effect transistors each comprising a source region and a drain region separated by a channel region and a gate positioned offset and spaced from the channel region. The biosensor also has one or more molecular probes coupled to at least one of the channel region and the offset gate, the one or more molecular probes configured to mate with at least one target. A method for detection of a target is also disclosed. One or more targets are immobilized as an electric field shunt between an offset gate and a channel region for one or more biosensors. A target measurement value is determined in proportion to a number of the one or more biosensors having the electric field shunt. | 01-26-2012 |
20120021403 | Human Endogenous Retrovirus with Foamy-Like Properties and Uses Thereof - The invention relates to the discovery of a human endogenous retrovirus (HERV) family, Type I HERV-K (HML-2) which appear to be active in vitro and in vivo, infectious, and which have the have the salient features and properties of foamy retroviruses. Based on its natural replication in humans, and that it protects the host from viral and tumor transformation, this non-pathogenic endogenous virus could be developed as a replication competent gene therapy vector. It also is expected to have much higher efficacy than other vectors as it crosses the bloodbrain barrier and infects almost all cell types in the host (proliferating or not). It may naturally lyse tumor cells or infected cells, and thus could even be used without genetic modification. Of course, this vector could be used in traditional ways with it ability to replicate genetically removed. In addition to its value as a vector, as it is reactivated with infection, its detection could also be used to monitor the safety of gene therapy (irrespective of vector type used), as well as other biological therapies including vaccination, blood transfusion, transplantation and xenotransplantation. Finally it may be used to screen for new therapeutic and prophylactic treatments for a wide variety of diseases. | 01-26-2012 |
20120021404 | Method for Diagnosis and Monitoring of Viral Infection by Analysis of Viral Transrenal Nucleic Acids in Urine - The present invention relates to methods for diagnosis or monitoring of viral infection by detecting the presence of transrenal viral nucleic acids or nucleic acids of viral origin in urine sample, with or without isolation of nucleic acids from a urine sample. The analysis of the nucleic acids is performed through hybridization of the nucleic acids with specific probes, or through a chain amplification reaction with specific primers. The methods are applicable to all viral pathogenic agents, including RNA, DNA, episomal, or integrated viruses. | 01-26-2012 |
20120021405 | Single Chain Antibody for the Detection of Noroviruses - The present invention concerns compositions and methods for detecting Norovirus or Norovirus particles. In particular, the present invention encompasses antibodies for detecting Norovirus or Norovirus particles, including, for example, monoclonal antibodies that have broad specificity of binding to various genogroups of norovirus. | 01-26-2012 |
20120021406 | METHOD FOR MONITORING A STERILIZATION PROCESS - The disclosed invention relates to a method for monitoring a sterilization process. The method comprises: (A) exposing an article to be sterilized and a biological indicator to a sterilization medium during a sterilization process, the biological indicator comprising a cell with a plasma membrane; and (B) measuring the membrane potential of the cell to detect the viability of the cell. | 01-26-2012 |
20120021407 | Methods and Devices for Rapid Urine Concentration - The present invention provides a device for the concentration of one or more target analytes contained in a urine sample. The device comprises a tube comprising an upper portion defining an opening for receiving the urine sample and a lower tapered portion terminating in collection reservoir. The tube contains a predetermined amount of a particulate binding agent which specifically binds the one or more target analytes and of a predetermined amount of a binding buffer. The device comprises means for seating the opening of the tube. The present invention further provides methods and kits for concentrating one or more target analytes in murine sample. | 01-26-2012 |
20120028242 | BIOSENSOR FOR DETECTING MULTIPLE EPITOPES ON A TARGET - The present invention encompasses a method for detecting a target comprising a repeating epitope. | 02-02-2012 |
20120028243 | AMPLIFICATION GENIQUE STATISTIQUE POUR L'IDENTIFICATION SANS A PRIORI DE MICRO-ORGANISMES PAR SEQUENCAGE SANS ETAPE DE CLONAGE - A pair of hexamers and a pair of primers specifically for identifying, without preconceptions, microorganisms in a sample, and a method for identifying one or more microorganisms, and a microorganism identification kit that uses the abovementioned hexamers and primers. | 02-02-2012 |
20120028244 | Anti-Viral Azide Containing Compounds - Methods of using azide-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to inhibit infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. Also, provided are methods of tracking a virus in vivo, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. The azide-modified biomolecules may be combined with a pharmaceutically acceptable excipient to produce a pharmaceutical composition, optionally containing another anti-viral agent and/or a delivery agent, such as a liposome. | 02-02-2012 |
20120028245 | Quantitative analyte assay device and method - The present invention relates to a quantitative assay device and a method for the determination of an analyte, based on a test strip, which contains a porous test membrane allowing for capillary flow of the analyte and complexes of the analyte, a porous upstream membrane in fluid connection with the test membrane and a porous downstream membrane in fluid connection with the test membrane, wherein the test membrane contains two bands having deposited on there high and low concentrations of different calibrator agents and a test band capable of reacting with conjugated analyte complexes giving rise to a measurable signal. | 02-02-2012 |
20120028246 | KIT FOR DETECTING HIGHLY PATHOGENIC AVIAN INFLUENZA VIRUS SUBTYPE H5N1 - Disclosed by the invention are an immunoassay kit and an immunoassay method for detecting highly pathogenic avian influenza virus subtype H5N1 rapidly, conveniently and specifically. Also disclosed are an immunochromatographic detection kit and an immunochromatographic detection method for detecting the virus subtype H5N1 rapidly, conveniently and specifically. It is found that a monoclonal antibody 4G6 produced by using the virus subtype H5N1 as an immunogen does not react with the subtype H5N2 virus or a subtype H5N3 virus and reacts only with a subtype H5N1 virus specifically. It is also found that only an avian influenza virus subtype H5N1 can be detected specifically by an immunoassay utilizing the monoclonal antibody 4G6. It is further found that the sensitivity of the detection of immunochromatography can be increased by adding a nonionic surface and a water-soluble vinyl polymer having a polar group containing an oxygen atom and a nitrogen atom to a developing solution to be used in the immunochromatography. | 02-02-2012 |
20120028247 | PLASMON SENSOR AND MANUFACTURING METHOD THEREFOR, AND METHOD FOR INSERTING SAMPLE INTO PLASMON SENSOR - A plasmon sensor includes a first metal layer and a second metal layer having an upper surface facing a lower surface of the first metal layer. The upper surface of the first metal layer is configured to receive an electromagnetic wave. A hollow space is provided between the first and second metal layers, and is configured to be filled with a test sample containing a medium. This plasmon sensor has a small size and a simple structure. | 02-02-2012 |
20120034597 | METHODS OF MONITORING TREATMENT OF AVIREMIC HIV-INFECTED PATIENTS - Methods of monitoring the efficacy of intensified highly active anti-retroviral therapy (HAART) treatment in aviremic Human Immunodeficiency Virus (HIV)-infected patients. | 02-09-2012 |
20120034598 | Real-Time Detection of Influenza Virus - The present invention provides system and methods for detecting an analyte indicative of an influenza viral infection in a sample of bodily fluid. The present invention also provides for systems and method for detection a plurality of analytes, at least two of which are indicative of an influenza viral infection in a sample of bodily fluid. | 02-09-2012 |
20120034599 | SCREENING SYSTEMS UTILIZING RTP801 - RTP801 represents a unique gene target for hypoxia-inducible factor-1 (HIF-1). Down-regulation of the mTOR pathway activity by hypoxia requires de novo mRNA synthesis and correlates with increased expression of RTP801. | 02-09-2012 |
20120034600 | DECREASING POTENTIAL IATROGENIC RISKS ASSOCIATED WITH INFLUENZA VACCINES - Influenza viruses for use in preparing human vaccines have traditionally been grown on embryonated hen eggs, although more modern techniques grow the virus in mammalian cell culture e.g. on Vero, MDCK or PER.C6 cell lines. The inventor has realised that the conditions used for influenza virus culture can increase the risk that pathogens other than influenza virus may grow in the cell lines and have identified specific contamination risks. Suitable tests can thus be performed during manufacture in order to ensure safety and avoid iatrogenic infections. | 02-09-2012 |
20120040334 | Diagnostic Transcript and Splice Patterns of HPV16 in Different Cervical Lesions - The present invention relates to a method for differentiating in a subject with HPV16 between (i) a severe form of HPV16 infection and (ii) a mild form of HPV16 infection based on determining the amount of a first gene product and a second gene product in a sample of a subject and calculating a ratio of the amount of said first gene product and the amount of said second gene product. Further envisaged by the present invention is a composition comprising an oligonucleotide mixture. Also envisaged by the present invention are a kit and a device adapted to carry out the method of the present invention. | 02-16-2012 |
20120040335 | Identification of Porcine Reproductive and Respiratory Syndrome Virus - An enzyme-linked immunosorbent assay (ELISA) is based on the non-structural protein 7 (nsp7) of porcine reproductive and respiratory syndrome virus (PRRSV) and provides for the simultaneous detection and differentiation of serum antibodies directed against Type 1 (European) and Type 2 (North American) PRRSV. The invention provides a serological assay for the detection and/or differentiation of serum antibodies directed against Type 1 and/or Type 2 PRRSV utilizing PRRSV nsp7 as an antigen, and provides a diagnostic method for the detection of PRRSV infection, epidemiological surveys, and outbreak investigations. The invention may be used either alone or as a follow-up assay to determine the true status of unexpected positive results that may occur using other assays, such as the IDEXX HERDCHEK PRRS ELISA. | 02-16-2012 |
20120040336 | Lateral Flow Assays for Non-Diagnostic Analytes - Methods of determining whether a non-diagnostic analyte is present in a non-diagnostic sample are provided. Aspects of the methods include applying a non-diagnostic sample to a sample receiving region of a lateral flow assay device and reading a detection region to determine whether a non-diagnostic analyte is present in the non-diagnostic sample. Also provided are kits that find use in practicing methods of the invention. | 02-16-2012 |
20120040337 | Conformational Epitope Initiated Signal Amplification - This invention relates to a method to generate a signal used to detect the presence or quantity of a biomarker in a sample. The signal generating reaction is initiated when the biomarker under assay interacts with a specific binding partner. The interaction produces a structural change in the binding partner that is recognized by additional binding partners capable of generating a signal. The reaction produces a localized cluster of signaling molecules that can be detected above background. The signaling cluster is detectable within minutes when interrogated in a chamber of specific dimensions. The presence of the signaling clusters is a qualitative indication of the presence of the analyte, while the number of signaling clusters detected is a direct quantification of the number of biomarker molecules in the sample. The reaction can be formatted to detect proteins, nucleic acids, cells or other informative biomarkers. | 02-16-2012 |
20120045747 | KIT FOR DETECTING HEPATITIS B VIRUS AND METHOD FOR DETECTING HEPATITIS B VIRUS USING THE SAME - A kit for detecting HBV in a test sample is disclosed. In addition a method is described for the real-time detection of HBV in a test sample using the kit. According to method of detection, the results of the detection can be rapidly identified with a reduced number of copies of a sample in real-time. | 02-23-2012 |
20120045748 | Particulate labels - A methodology for bioassays and diagnostics in which a particulate label (ranging in size from nm-scale molecular assemblages to organisms on the scale of tens or hundreds of microns), such as, but not limited to, nanoparticles, bacteria, bacteriophage, Daphnia, and magnetic particles, serve carriers for analytes bound by molecular recognition elements such as antibodies, aptamers, etc. The described methodology is generally applicable to most pathogen assays and molecular diagnostics and also leads to enhanced sensitivity and convenience of use. | 02-23-2012 |
20120045749 | METHODS OF CATEGORIZING AN ORGANISM - The invention generally relates to methods of identifying and categorizing organisms and more specifically methods of generating and using patterns of chromosomal variation in order to classify organisms. | 02-23-2012 |
20120045750 | KIT FOR DETECTING HIV-2 AND METHOD FOR DETECTING HIV-2 USING THE SAME - A kit for detecting HIV-2 strains in a test sample is disclosed. In addition a method is described for the real-time detection of HIV-2 strains in a test sample using the kit. According to method of detection, the results of the detection can be rapidly identified with a reduced number of copies of a sample in real-time. | 02-23-2012 |
20120045751 | Generic Sample Preparation - The present invention relates to the sample preparation of nucleic acids for diagnostic purposes. More precisely, the invention provides a process for simultaneously isolating at least a first and a second target nucleic acid from a plurality of different types of fluid samples and optionally amplifying said isolated nucleic acids in a simultaneous manner. | 02-23-2012 |
20120045752 | FIBER SAMPLER FOR RECOVERY OF BIOAEROSOLS AND PARTICLES - A bioparticle collection device and an aerosol collection system. The bioparticle collection device includes a collection medium including a plurality of fibers formed into a fiber mat and configured to collect bioparticles thereon, and includes a viability enhancing material provider disposed in a vicinity of the plurality of fibers and configured to provide a viability enhancing material to the collected bioparticles to maintain viability of the bioparticles collected by the fiber mat. The aerosol collection system includes an aerosol pumping device configured to entrain particles in an gas stream, an aerosol saturation device configured to saturate the particles in the gas stream with a biocompatible liquid, and an aerosol collection medium downstream from the aerosol saturation device and including a plurality of fibers formed into a fiber mat for collection of the saturated aerosol particles. | 02-23-2012 |
20120045753 | SEQUENCES DIAGNOSTIC FOR SHRIMP PATHOGENS - Primers have been isolated that are diagnostic for the detection of the white spot syndrome virus (WSSV). The primers are based on a new portion of the WSSV genome and may be used in primer directed amplification or nucleic acid hybridization assay methods. | 02-23-2012 |
20120045754 | NOVEL GOLD NANOSTRUCTURES AND METHODS OF USE - The invention is drawn to novel nanostructures comprising hollow nanospheres and nanotubes for use as chemical sensors, conduits for fluids, and electronic conductors. The nanostructures can be used in microfluidic devices, for transporting fluids between devices and structures in analytical devices, for conducting electrical currents between devices and structure in analytical devices, and for conducting electrical currents between biological molecules and electronic devices, such as bio-microchips. | 02-23-2012 |
20120058461 | MOLECULAR DETECTION OF XMRV INFECTION - The present invention relates generally to assays for the detection of Xenotropic Murine Leukemia Virus-related Retrovirus (“XMRV”) and diseases associated with XMRV infection. In particular, the invention relates to XMRV-related nucleic acids having significant diagnostic and screening utilities and methods of using the same. | 03-08-2012 |
20120058462 | MOLECULAR DETECTION OF XMRV INFECTION - The present invention relates generally to assays for the detection of Xenotropic Murine Leukemia Virus-related Retrovirus (“XMRV”) and diseases associated with XMRV infection. In particular, the invention relates to XMRV-related nucleic acids having significant diagnostic and screening utilities and methods of using the same. | 03-08-2012 |
20120058463 | HYDROPHOBIC, FUNCTIONALIZED PARTICLES - The present invention relates to a stable mixture comprising surface-modified particles which are obtained by reacting metal oxide or semimetal oxide particles with at least one compound selected from among silicon-comprising compounds bearing one, two or three alkoxy radicals and at least one solvent, at least one surface-active substance or a mixture thereof, the use of these particles in systems in which they are brought into contact with at least one solvent, where the mass ratio of solvent to modified particle is greater than 500, and also the use of these particles in agglomeration-deagglomeration cycles. | 03-08-2012 |
20120058464 | Assay Methods Using Array of Test Zones - A method for assaying a sample for each of multiple analytes is described. The method includes contacting an array of spaced-apart test zones with a liquid sample (e.g., whole blood). The test zones disposed within a channel of a microfluidic device. The channel is defined by at least one flexible wall and a second wall which may or may not be flexible. Each test zone comprising a probe compound specific for a respective target analyte. The microfluidic device is compressed to reduce the thickness of the channel, which is the distance between the inner surfaces of the walls within the channel. The presence of each analyte is determined by optically detecting an interaction at each of multiple test zones for which the distance between the inner surfaces at the corresponding location is reduced. The interaction at each test zone is indicative of the presence in the sample of a target analyte. Capillary structures of the devices or used in the methods may comprise a matrix and the devices may comprise control elements and methods for assaying of sample may use corresponding controlling activities. | 03-08-2012 |
20120058465 | METHOD AND DEVICE FOR ASSAY - A device for assay can evenly develop solution, and performs highly accurate and sensitive measurement. A first device part ( | 03-08-2012 |
20120058466 | DIFFERENTIAL IMMUNOASSAY FOR PRRS VACCINE ANTIBODY - The present invention relates to immunoassays for serologically differentiating animals naturally infected with PRRS virus from animals vaccinated against PRRS. The immunoassays provide detection of at least a portion of the N terminal region of the 2b portion of PRRSV. The immunoassay is preferably an enzyme-linked immunosorbent assay (ELISA). | 03-08-2012 |
20120064509 | Methods and compositions related thereto for detecting and identifying distinct species of nucleic acids from causative agents - Methods are described herein for detecting and identifying distinct species of nucleic acids, in a single container, for example, from a certain genus of infectious agents or otherwise causative agents comprising, for example, providing a forward PCR primer common to a homologous gene region between the distinct species, and providing a reverse PCR primer common to a homologous gene region between the distinct species, to thereby define a PCR target region amongst the species, and providing a first oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a first species, providing a second oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a second species, wherein the first and second oligonucleotide probes are each detectably labeled with distinctly different detectable labels, conducting a PCR reaction in the container by means of the primers to amplify the target region amongst the species, and detecting the distinct labels, thereby identifying distinct species of nucleic acids corresponding to distinct species of infectious agents. Methods are preferred, for example, wherein the infectious agent is a member of the Herpesviridae family. | 03-15-2012 |
20120064510 | KIT INCLUDING TARGET SEQUENCE-BINDING PROTEIN AND METHOD OF DETECTING TARGET NUCLEIC ACID BY USING THE KIT - A kit including a target sequence-binding protein and a method of detecting a target nucleic acid by using the kit that may ensure efficient detection of the target nucleic acid in a biological sample are disclosed. | 03-15-2012 |
20120064511 | Generic Buffer For Amplification - The present invention relates to a method of amplifying a first and a second target nucleic acid in separate reaction receptacles, wherein said reaction receptacles comprise a solution comprising amplification reagents and oligonucleotides specific for said first or said second target nucleic acid, wherein said solution is the same for amplifying said first target nucleic acid and said second target nucleic acid. | 03-15-2012 |
20120064512 | GENETIC POLYMORPHISMS ASSOCIATED WITH LIVER FIBROSIS, METHODS OF DETECTION AND USES THEREOF - The present invention is based on the discovery of genetic polymorphisms that are associated with liver fibrosis and related pathologies. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, including groups of nucleic acid molecules that may be used as a signature marker set, variant proteins encoded by such nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and proteins, and methods of using the nucleic acid and proteins as well as methods of using reagents for their detection. | 03-15-2012 |
20120064513 | Cell Sensor, And Monitoring Method Using Same For The Real-Time Monitoring Of Cell Capacitance - The present invention relates to a cell sensor for real-time monitoring of cell capacitance and a monitoring method using the same, and more particularly, to a cell sensor capable of monitoring an endocytosis process of a biomolecule through a cell surface receptor by attaching a cell between electrodes and then measuring in real time the capacitance between the electrodes over time, and a monitoring method using the same. A cell sensor for the real-time monitoring of cell capacitance according to an exemplary embodiment of the present invention includes a substrate; a first electrode and a second electrode that are formed on the substrate and spaced apart by a gap from each other, in which at least one or more cells are introduced to be attached onto the gap; and a passivation layer that is formed on each of the tops of the first electrode and the second electrode to prevent the cells from being attached onto the top of the first electrode and the second electrode. The cell sensor for the real-time monitoring of cell capacitance and the method for real-time monitoring of the cell condition using the same according to an exemplary embodiment of the present invention have the effect of being able to monitor an endocytosis process of a biomolecule through a cell surface receptor in real time, by attaching cells having a receptor for the particular biomolecule between electrodes and then measuring in real time the capacitance between the electrodes over time. | 03-15-2012 |
20120064514 | HIV-1-C RESISTANCE MONITORING - The present invention relates to methods for the evaluation of HIV-1 Subtype C (HIV-1-C) treatment. The methods are based on evaluating molecular events at the HIV-1-C gag-protease-reverse transcriptase (GPRT) resulting in altered therapeutic efficacy of investigated anti-retroviral compounds. The methods rely on providing HIV-1-C GPRT RNA and evaluating a treatment either through genotyping or phenotyping methods. Said methods may find a use in the field of diagnostics, drug screening, pharmacogenetics and drug development. | 03-15-2012 |
20120070819 | Determination of Interactions of Constant Parts of Antibodies with FC-Gamma Receptors - The invention relates to a novel method for the exact determination of the binding of the Fc-part of IgG-antibodies to Fc-gamma receptors, and for the simultaneous examination of the antigen-specificity and the Fc-gamma-receptor activation, as well as specific materials for use in said method. The invention furthermore relates to a method for identifying substances that affect the binding of the Fc-part of IgG-antibodies to Fc-gamma receptors, on the basis of the method for the exact determination of the binding of the Fc-part. | 03-22-2012 |
20120070820 | Probes and Methods for Hepatitis C Virus Typing Using Multidimensional Probe Analysis - This invention provides compositions and methods for HCV typing, e.g., genotyping and/or subtyping. The compositions and methods of the invention can be used to assign an HCV isolate to one of at least five HCV types (for example, selected from types 1, 2, 3, 4, 5 or 6), or to one of at least five subtypes (for example, subtypes 1a/b/c, 2a/c, 2b, 3a, 4a, 5a or 6a). These methods integrate the hybridization data from a plurality of HCV typing probes in a multidimensional analysis to make an HCV type assignment for an HCV in an experimental sample. The invention also provides related compositions, including, for example, the HCV typing probes and HCV typing diagnostic kits. | 03-22-2012 |
20120070821 | Interference Control Panel for Evaluation of Analytical Assays For Samples Derived from Blood - The invention relates to quality control of analytical assays, particularly NAT assays of blood samples containing nucleic acids. A control panel containing quantified amounts of substances known to interfere with an analytical assay is used and compared with a reference sample in the analytical assay. A comparison of the assay results interference panel validates the assay and can serve as a periodic quality control check for the analytical assay as well as related methods and protocols. The use of the control panel of the invention can also determine whether interfering substances are present and establish under what conditions the analytical assay reliable. | 03-22-2012 |
20120070822 | METHOD FOR SIGNAL AMPLIFICATION DURING LATERAL-FLOW ANALYSIS - Provided is a signal amplifying method in a lateral flow analysis with high sensitivity, in which gold ions and a reductant are added and react to a seed of gold nano particles to amplify a signal, and a lateral flow analysis device using the same. | 03-22-2012 |
20120077184 | ELECTROMAGNETIC MULTIPLEX ASSAY BIOSENSOR - A method is provided for determining the presence of multiple different target analytes in a liquid sample using electrophoretic separation and magnetic labels within a self-contained reaction cartridge and an external magnetic sensor for detection. Magnetic labels are bound to target analytes through specific binding elements. By electrophoretic separation, the multiple different targets can be sorted according to their specific sizes and inherent molecular charges for better detection resolution and specificity. After the separation process, the target analytes are then recognized and trapped by the detection binding elements within the reaction cartridge. A magnetic field generator provides a changeable magnetic field that causes the bounded magnetic labels and target analytes to produce a resonance disruption detectable by a magnetic sensor. The sensor can provide a digital binary value to indicate whether or not a label particle is bound and that determines the presence of target analytes. | 03-29-2012 |
20120077185 | DETECTION OF GENETIC ABNORMALITIES AND INFECTIOUS DISEASE - The present invention provides assay systems and related methods for detecting genetic abnormalities and infectious agents in maternal samples. Exemplary maternal samples for analysis using the assay systems of the invention include maternal blood, plasma or serum. | 03-29-2012 |
20120088226 | NUCLEIC ACID EXTRACTION METHOD - The present invention relates to a nucleic acid extracting apparatus, and the nucleic acid extracting apparatus can include a pipe-shaped tube having an open outlet at one side thereof, and a hydrogel column that is provided inside the tube and filters impurities excluding an extraction target material. | 04-12-2012 |
20120088227 | Devices and Process for Separating Plasma From a Blood Sample - The present invention pertains to a device for separating plasma from a blood sample comprising a stacked structure which is provided with a first portion including a separating member having a first surface for applying or receiving the blood sample, wherein the separating member is adapted to permit the passage of plasma but to inhibit the passage of cells, and a second portion including an absorptive member for absorbing the plasma, which has a second surface in contact with the separating member for receiving the plasma, wherein the absorptive member is adapted to generate a capillary pressure so as to draw plasma from the separating member to the absorptive member. The first portion is fixed to the backing member in a manner to be removed without destroying the absorptive member. The absorptive member is fixed to the backing member in a manner to be removed without destroying the absorptive member. | 04-12-2012 |
20120088228 | METHODS OF PRODUCING HIGH TITER, HIGH PURITY VIRUS STOCKS AND METHODS OF USE THEREOF - The purity and titer of virus stocks used for virus clearance studies have a significant influence on study outcome, and impact how well the scale-down model represents the production-scale process. Impurities in virus stocks are particularly important in the testing of small virus retentive filters because these impurities may cause a filter to foul prematurely, leading to underestimation of the true throughput capability of the filter and consequent inappropriate sizing of the production scale unit. In addition, impurities can also affect the levels of virus clearance observed by altering the fouling mechanisms and subsequent fluid passage through the virus filter. Described herein are methods for making, producing and using high purity virus stocks having high titer. | 04-12-2012 |
20120088229 | SURFACE PLASMON RESONANCE SENSOR - The application relates to a sensor using a gold layer ( | 04-12-2012 |
20120088230 | System And Method For Cell Analysis - A system for enumeration of objects such as cells in a sample is disclosed. The system uses a low-cost cartridge and a reader instrument, based on planar waveguide imaging technology. Cells of a blood sample may be stained with fluorescence-tagged antibodies and are loaded onto the cartridge where the differentially labeled cells may be distinguished and quantified. | 04-12-2012 |
20120088231 | Biological Specimen Collection/Transport Compositions and Methods - Disclosed are compositions for collecting, storing, and transporting populations of nucleic acids from biological specimens, and clinical, forensic, or environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. In particular embodiments, the invention provides a single, one-step, sample collection/transport/storage formulation containing a known quantity of a non-genomic, nucleic acid carrier molecule that serves as an internal reference control to monitor the fidelity of the collection/transportation medium, and measure the integrity of nucleic acids subsequently isolated and purified from the processed sample. | 04-12-2012 |
20120094270 | SELF-EXCITING, SELF-SENSING PIEZOELECTRIC CANTILEVER SENSOR FOR DETECTION OF AIRBORNE ANALYTES DIRECTLY IN AIR - A method for detection of airborne biological agent using a piezoelectric cantilever sensor that includes a piezoelectric layer and a non-piezoelectric layer. A recognition entity is placed on one or both of the two layers. The antibody that recognizes and binds to the airborne species may be chemically immobilized on the cantilever sensor surface. In one embodiment, the cantilever sensor is attached to a base at only one end. In another embodiment, the sensor includes first and second bases and at least one of the piezoelectric layer and the non-piezoelectric layer is affixed to each of the first and second bases to form a piezoelectric cantilever beam sensor. In this embodiment, resonance is measured via stress on the piezoelectric layer and it has been demonstrated that such sensors are robust and exhibit excellent sensing characteristics in gaseous media with sufficient sensitivity to detect airborne species at relatively low concentrations. | 04-19-2012 |
20120094271 | SELECTING FOR COOPERATIVELY INTERACTING MOLECULES - The present invention provides method of identifying molecules that cooperatively and positively interact with either a ligand or a target molecule of a ligand/target molecule pair, or molecules that interact with a ligand/target molecule complex. | 04-19-2012 |
20120094272 | SIGNAL ENHANCEMENT SYSTEM WITH MULTIPLE LABELED-MOIETIES - Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labelled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labelled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labelled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described. | 04-19-2012 |
20120094273 | IMMUNOLOGICAL METHOD FOR DETECTING ACTIVE JCV INFECTION - The invention relates to an immunological method for detecting an extra renal active infection by JC virus in a patient candidate for a treatment with an immunosuppressive treatment or during the course of this treatment. | 04-19-2012 |
20120094274 | IDENTIFICATION OF SWINE-ORIGIN INFLUENZA A (H1N1) VIRUS - The present invention provides oligonucleotide primers, compositions, and kits containing the same for rapid identification of viruses (e.g., swine-origin influenza A (H1N1) virus) which are members of the influenza virus family by amplification of a segment of viral nucleic acid followed by molecular mass analysis. | 04-19-2012 |
20120094275 | METHODS AND KITS FOR THE DETECTION OF CIRCULATING TUMOR CELLS IN PANCREATIC PATIENTS USING POLYSPECIFIC CAPTURE AND COCKTAIL DETECTION REAGENTS - A highly sensitive assay is disclosed which combines immunomagnetic enrichment with multiparameter flow cytometric or image cytometry to detect, enumerate and characterize carcinoma cells in the blood. The present invention incorporates the conjugation of different antibodies to the same ferrofluid. This has the effect of making the ferrofluid polyspecific with respect to the antigens that the ferrofluid will bind. The multiple antibodies present on the same ferrofluid do not appear to block or otherwise interfere with each other. Such ferrofluids have the highly desirable effect of being able to bind specifically to more than one type of cell. The assay is especially useful to enable the capture of CTCs that have low EpCAM expression, but high expression of other tumor markers; Accordingly, the assay facilitates the biological characterization and staging of carcinoma cells. | 04-19-2012 |
20120094276 | METHODS AND DEVICES TO ENHANCE SENSITIVITY AND EVALUATE SAMPLE ADEQUACY AND REAGENT REACTIVITY IN RAPID LATERAL FLOW IMMUNOASSAYS - Methods and devices for rapid lateral flow immunoassays to detect specific antibodies within a liquid sample while also validating the adequacy of the liquid sample for the presence of immunoglobulin and the integrity and immunoreactivity of the test reagents that detect the antibodies of interest, without requiring instrumentation. The methods and devices provide for delivery of a diluted liquid sample to a single location that simultaneously directs the liquid flow along two or more separate flow paths, one that serves as a positive control to confirm that all critical reagents of the test are immunoreactive, and that the sample being tested is adequate, and the other to detect specific antibodies if present. | 04-19-2012 |
20120094277 | Methods of producing competitive aptamer FRET reagents and assays - Methods are described for the production and use of fluorescence resonance energy transfer (FRET)-based competitive displacement aptamer assay formats. The assay schemes involve FRET in which the analyte (target) is quencher (Q)-labeled and previously bound by a fluorophore (F)-labeled aptamer such that when unlabeled analyte is added to the system and excited by specific wavelengths of light, the fluorescence intensity of the system changes in proportion to the amount of unlabeled analyte added. Alternatively, the aptamer can be Q-labeled and previously bound to an F-labeled analyte so that when unlabeled analyte enters the system, the fluorescence intensity also changes in proportion to the amount of unlabeled analyte. The F or Q is covalently linked to nucleotide triphosphates (NTPs), which are incorporated into the aptamer by various nucleic acid polymerases, such as Taq or Deep Vent Exo | 04-19-2012 |
20120100526 | IDENTIFICATION AND DIFFERENTIATION OF NUCLEIC ACID SEQUENCE USING TEMPERATURE-DEPENDENT HYBRIDIZATION - Oligonucleotide probes are provided that are capable of hybridizing to different target nucleic acid sequences in a temperature-dependent manner allowing for detection of more than one target sequence by the same probe or by different probes having reporter labels with identical or similar detection characteristics. Also provided is a method for detecting target nucleic acid sequences using an oligonucleotide probe capable of hybridizing to the sequences in a temperature-dependent manner or using different probes having reporter labels with identical or similar detection characteristics. | 04-26-2012 |
20120100527 | METHOD OF DETECTING H5 OR H7 AVIAN INFLUENZA VIRUS - The present invention provides oligonucleotide primers specifically hybridizing to an arbitrary nucleotide sequence designed from the nucleotide sequence of hemagglutinin of an H5 or H7 avian influenza virus, a nucleic acid amplification method using the primers, a method for diagnosis of infection with an H5 or H7 avian influenza virus by detection of nucleic acid amplification, and a kit for influenza diagnosis. | 04-26-2012 |
20120100528 | METHOD FOR DETECTION OF HUMAN PAPILLOMAVIRUS (HPV) TYPE - The present invention describes a method for detection of human papillomavirus (HPV) types and a kit for detection of said HPV types. | 04-26-2012 |
20120100529 | Biological Specimen Collection and Transport System and Methods of Use - Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. The disclosed compositions safely facilitate rapid sample collection, and provide extended storage and transport of the samples at ambient or elevated temperature without contamination of the sample or degradation of the nucleic acids contained therein. This process particularly facilitates the collection of specimens from remote locations, and under conditions previously considered hostile for preserving the integrity of nucleic acids released from lysed biological samples without the need of refrigeration or freezing prior to molecular analysis. | 04-26-2012 |
20120107794 | Cross-Coupled Peptide Nucleic Acids for Detection of Nucleic Acids of Pathogens - The present invention concerns methods for detecting a nucleic acid comprising (i) contacting a solution comprising a first PNA having a first cross-reactive functional group with a substrate having a second PNA affixed thereto, the second PNA having a second first cross-reactive functional group, wherein the first PNA has a reporter molecule attached thereto and the first and second PNAs being complementary to different portions of a target DNA; (ii) contacting a sample suspected of containing the nucleic acid with the first and second PNAs; and (iii) determining the presence of the reporter molecule on the substrate. | 05-03-2012 |
20120107795 | Methods For Concurrent Identification And Quantification Of An Unknown Bioagent - The present invention provides methods for the quantification of an unknown bioagent in a sample by amplification of nucleic acid of the bioagent, and concurrent amplification of a known quantity of a calibration polynucleotide from which are obtained a bioagent identifying amplicon and a calibration amplicon. Upon molecular mass analysis, mass and abundance data are obtained. The identity of the bioagent is then determined from the molecular mass of the bioagent identifying amplicon and the quantity of the identified bioagent in the sample is determined from the abundance data of the bioagent identifying amplicon and the abundance data of the calibration amplicon. | 05-03-2012 |
20120107796 | METHODS AND KITS TO DETECT NEW H1N1 "SWINE FLU" VARIANTS - Methods and kits used in the detection of the H1N1/09 influenza virus are disclosed. The methods comprise methods of using the nucleic acids to detect H1N1/09 generally as well as H1N1/09 variants resistant to antiviral compositions. | 05-03-2012 |
20120107797 | GENOTYPING OF BOVINE PAPILLOMAVIRUS GENOTYPES - The present invention is concerned with the provision of diagnostic means and methods. Specifically, it relates to a composition comprising oligonucleotides selected from at least two different groups of oligonucleotides, said groups comprising at least one pair of oligonucleotides being capable of specifically amplifying polynucleotides comprised by a Bovine Papillomavirus (BPV), said BPV being selected from the group consisting of BPV-1, BPV-2, BPV-3, BPV-4, BPV-5, BPV-6, BPV-7, BPV-8, BPV-9, BPV-10, and BAPV-11 as well as uses based on said composition and kits comprising it. Moreover, contemplated is a method for the simultaneous detection and/or identification of BPV types in a sample. | 05-03-2012 |
20120107798 | SINGLE MOLECULE SENSITIVE PROBES FOR DETECTING RNA - The various embodiments of the present disclosure relate generally to single molecule sensitive probes for detecting RNA, and more particularly to multivalent fluorescent probes for detecting a single molecule of RNA in a cell. The present invention includes a RNA imaging probe comprising: a multivalent core comprising a plurality of attachment sites; a plurality of RNA/DNA chimeric oligonucleotides having a specificity for a target RNA, wherein a RNA/DNA chimeric oligonucleotide is bound to an attachment site of the multivalent core; and a plurality of fluorophores, wherein a fluorophore is bound to the RNA/DNA chimeric oligonucleotide. | 05-03-2012 |
20120115125 | ASSAY FOR DETECTING AND QUANTIFYING HIV-1 - Method of detecting HIV-1 nucleic acids using nucleic acid amplification and a molecular torch hybridization probe. The invented method is characterized by high levels of precision in the quantitation of HIV-1 targets at low copy numbers, and by accurate detection of different HIV-1 subtypes, including M group and O group variants. | 05-10-2012 |
20120115126 | Compositions and Methods for Rapid, Real-Time Detection of Influenza A Virus (H1N1) Swine 2009 - Disclosed are oligonucleotide amplification primers and detection probes specific for the amplification and detection of pathogenic organisms, including for example, specific Influenza A H1N1 viral isolates. Also disclosed is a biological organism identification kit including the disclosed nucleic acid probes and primers, as well as thermal cycling reagents that is both portable and durable, and may also be self-contained for remote, or in-field analysis and identification of particular influenza isolates from a variety of biological specimen types. | 05-10-2012 |
20120122078 | METHODS AND SYSTEMS FOR PREDICTING WHETHER A SUBJECT HAS A CERVICAL INTRAEPITHELIAL NEOPLASIA (CIN) LESION FROM A SUSPENSION SAMPLE OF CERVICAL CELLS - Methods of predicting whether a subject has a cervical intraepithelial neoplasia (CIN) lesion are provided. Aspects of the methods include obtaining both morphometric and biomarker data from a liquid cervical cellular sample and then using both types of data to predict whether the subject has a CIN lesion. Also provided are systems that find use in practicing the methods. The methods and systems find use in a variety of applications, including cervical cancer screening applications. | 05-17-2012 |
20120122079 | INFECTION DETECTION METHODS AND SYSTEMS AND RELATED COMPOUNDS AND COMPOSITIONS - A compound, or a pharmaceutically acceptable salt, ester, hydrate or solvate thereof, comprising formula IV: | 05-17-2012 |
20120122080 | FLUORESCENCE ENERGY TRANSFER BY COMPETITIVE HYBRIDIZATION - A method is provided for detecting the presence of nucleotides or monitoring nucleotide amplification. It utilizes fluorescence energy transfer by competitive hybridization. Competitive hybridization is achieved by using unequal length complementary probes which have a fluorophore on one probe and a quencher on the other. The fluorophore and quencher are juxtaposed in a manner wherein the proximity of the quencher to the fluorophore produces quenching of the fluorescence of the fluorophore. | 05-17-2012 |
20120122081 | DIFFERENTIATING PICORNA VIRUSES, NUCLEIC ACIDS THEREFOR, USE THEREOF AND BIOASSAY METHODS EMPLOYING THEM - A nucleic acid comprising a 13 base sequence selected from the group consisting of tcGg TtccgCt Gc, tcGgTtccgCc Ac, tcGgTcCcaTcCc, tcGgTtCcaTcCc, ttGgTcCcaTcCc, ttGgTtCcaTcCc, tcGgTcccgTcCc, and tcGgTtccgTcCc, where A and a are adenine, C and c are cytosine, G and g are guanine, and T and t are thymine or uracil, and complementary sequences thereof; provided that only a very limited number of additional bases are included at the 5′ and 3′ ends of the sequences. The use of these nucleic acids for differentiating picornaviruses, and bioassay methods employing these nucleic acids in nucleic acid amplification assays, are also disclosed. | 05-17-2012 |
20120122082 | METHOD AND KIT FOR DETECTION OF MICROORGANISM - Live cells of microorganism in a test sample are detected by distinguishing the live cells from dead cells or injured cells by the following steps of:
| 05-17-2012 |
20120129151 | Quantitative Real-Time Assay For Noroviruses And Enteroviruses With Built In Quality Control Standard - A method is provided for reverse transcription-polymerase chain reaction (RT-PCR) accomplished by: a) amplifying a reverse transcribed cDNA in a mixture containing Norovirus Genogroup I and Norovirus Genogroup II primers and probes, in which the Norovirus primers and probes can distinguish between Genogroup I and Genogroup II viruses; b) quantifying virus; and c) normalizing data based on a universal internal RNA control. Optionally, the method may also include primers and probes for Enteroviruses. The present invention also provides a reaction mixture comprising containing Norovirus Genogroup I and Norovirus Genogroup II primers and probes, in which the Norovirus primers and probes can distinguish between Genogroup I and Genogroup II viruses and universal internal RNA control primers and probes. | 05-24-2012 |
20120129152 | Diagnostic method for the prediction of the development of and control over the effectiveness of treatment of cardiovascular illnesses - A diagnostic method for the prediction of the development and control of the effectiveness of the treatment of cardiovascular diseases, in which patient tissue samples are taken, microassay are prepared, specific antiviral immunoglobulins are processed, the number of cells infected by two or more viruses before the beginning of treatment are determined, and the dynamic of the change in the number of infected cells and their interrelationships are established: when the number of cells infected by cytomegalovirus and any other viruses decreases by more than 50±10% in patients without symptoms of cardiovascular pathology, a diagnostic conclusion is high danger of the development of atherosclerosis; if the number of cells infected by cytomegalovirus and any other viruses exceeds 50±10% in patients with demonstrated clinical signs of cardiovascular system pathology, a diagnostic conclusion is drawn of the danger of the development of complications such as arrhythmia, thrombolytic embolism. | 05-24-2012 |
20120129153 | Diagnostic method for the prediction of the development and control of the effectiveness of the treatment of oncological illnesses - A diagnostic method, in which patient tissue samples are taken, microassay are prepared, specific anti-viral immunoglobulins are processed, the number of cells infected by two or more viruses before the beginning of treatment are determined, and the dynamic of the change in the number of infected cells and their interrelationships are established: if the number of blood cells infected by any two or more viruses exceeds 50±10% in patients without signs of oncological pathology, a diagnostic conclusion is drawn of a high danger of oncological illness in connection with immune system ineffectiveness; if the number of cells infected by any two or more viruses exceeds 50±10% in patients with diagnosed oncological illnesses, a diagnostic conclusion of the cancer tumor's low sensitivity to chemotherapy and the perspective of quick tumor metastasis is drawn. | 05-24-2012 |
20120129154 | METHODS AND COMPOSITIONS FOR THE DETECTION OF BACTERIAL BLIGHT - Methods and compositions relating to the detection of bacterial blight are provided. In one embodiment, a detection system is provided comprising a phage operable to infect a | 05-24-2012 |
20120129155 | METHODS FOR AMPLIFYING HEPATITIS C VIRUS NUCLEIC ACIDS - A method of amplifying an HCV nucleic acid in an HCV infected sample comprises amplifying a segment of a DNA template that is complementary to a genome of HCV RNA from the sample by a two-stage PCR, wherein a first stage PCR employs a first outer primer and a second outer primer, and a second stage PCR employs a first inner primer and a second inner primer. The nucleotide sequence of the first outer primer comprises a nucleotide sequence as set forth in SEQ ID NO: 2; or SEQ ID NO:9, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 9. The nucleotide sequence of the second outer primer comprises a nucleotide sequence set forth in SEQ ID NO: 3 or 4; or a nucleotide sequence as set forth in SEQ ID NO: 10 or 11, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 10 and 11. The nucleotide sequence of the first inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 5; or SEQ ID NO:12, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 12. The nucleotide sequence of the second inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 6 or 7; or a nucleotide sequence as set forth in SEQ ID NO: 13 or 14, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 13 and 14. | 05-24-2012 |
20120129156 | BARRIERS FOR FACILITATING BIOLOGICAL REACTIONS - The present invention relates to systems, devices, and methods for performing biological reactions. In particular, the present invention relates to the use of lipophilic, water immiscible, or hydrophobic barriers in sample separation, purification, modification, and analysis processes. | 05-24-2012 |
20120129157 | Methods and Devices for Rapid Detection and Identification of Live Microorganisms by Aptamers and/or Antibodies Immobilized on Permeable Membranes - The invention provides methods, devices and kits for rapid detection and identification of one or more live target microorganisms in a liquid sample or grown on plates containing nutrient media. The invention includes mixing one or more target microorganisms with one or more aptamers and/or one or more antibodies, each conjugated to a reporter compound and specific for a first site on one or more target microorganisms to form a mixture. The mixture is placed on a permeable membrane having immobilized thereon one or more aptamers linked to an amine compound, and/or one or more antibodies, each specific for a second site on one or more target microorganisms or a site on the aptamer conjugate and/or antibody conjugate. A detection solution is added to the membrane and detection and identification of one or more target microorganisms is achieved in about one hour or less. | 05-24-2012 |
20120135393 | REAL-TIME PCR POINT MUTATION ASSAYS FOR DETECTING HIV-1 RESISTANCE TO ANTIVIRAL DRUGS - Disclosed are compositions including primers and probes, which are capable of interacting with the disclosed nucleic acids, such as the nucleic acids encoding the reverse transcriptase or protease of HIV as disclosed herein. Thus, provided is an oligonucleotide comprising any one of the nucleotide sequences set for in SEQ ID NOS:1-89, and 96-104. Also provided are the oligonucleotides consisting of the nucleotides as set forth in SEQ ID NOS:1-89, and 96-104. Each of the disclosed oligonucleotides is a probe or a primer. Also provided are mixtures of primers and probes and for use in RT-PCR and primary PCR reactions disclosed herein. Provided are methods for the specific detection of several mutations in HIV. Mutations in both the reverse transcriptase and the protease of HIV can be detected using the methods described herein. | 05-31-2012 |
20120135394 | APPARATUS FOR INTEGRATED REAL-TIME NUCLEIC ACID ANALYSIS, AND METHOD FOR DETECTING A TARGET NUCLEIC ACID USING SAME - Provided are an apparatus for integrated real-time nucleic acid analysis and a method for detecting target a nucleic acid using the same, and more particularly an integrated real-time nucleic acid analysis for simultaneously performing qualitative analysis or quantitative analysis on genes from various kinds of plural biological samples and a method for detecting target a nucleic acid using the same. The apparatus for integrated real-time nucleic acid analysis and the method for detecting target a nucleic acid using the same according to the present invention, perform tests of various targets required from various samples through a single step promptly and accurately, and thus, can be efficiently used by hospitals or the like needing to rapidly diagnose diseases. | 05-31-2012 |
20120135395 | Methods and Compositions for Determining Altered Susceptibility of HIV-1 to Anti-HIV Drugs - This invention relates, in part, to methods and compositions for determining altered susceptibility of a human immunodeficiency virus (“HIV”) to the non-nucleoside reverse transcriptase inhibitors (“NNRTIs”) efavirenz (“EFV”), nevirapine (“NVP”), and delavirdine (“DLV”), the nucleoside reverse transcriptase inhibitor AZT, and the integrase strand transfer inhibitors diketo acid 1, diketo acid 2, and L-870,810 by detecting the presence of a mutation or combinations of mutations in the gene encoding HIV reverse transcriptase that are associated with altered susceptibility to the anti-HIV drugs. | 05-31-2012 |
20120135396 | Multi-Shell Microspheres With Integrated Chromatographic And Detection Layers For Use In Array Sensors - The development of miniaturized chromatographic systems localized within individual polymer microspheres and their incorporation into a bead-based cross-reactive sensor array platform is described herein. The integrated chromatographic and detection concept is based on the creation of distinct functional layers within the microspheres. In this first example of the new methodology, complexing ligands have been selectively immobilized to create “separation” layers harboring an affinity for various analytes. Information concerning the identities and concentrations of analytes may be drawn from the temporal properties of the beads' optical responses, Varying the nature of the ligand in the separation shell yields a collection of cross-reactive sensing elements well suited for use in array-based micro-total-analysis systems. | 05-31-2012 |
20120135397 | HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 (HIV-1) DETECTION METHOD AND KIT THEREFOR - The invention provides oligonucleotide(s) derived from the gene sequence encoding the gag region of HIV-I for simple, specific and/or sensitive test(s) for the presence of HIV-I. In particular, the present invention provides oligonucleotide(s) for test(s) for HIV-I. Kit(s) comprising the oligonucleotide(s) for use as probe(s) and/or primer(s) useful in the test(s) are also provided. | 05-31-2012 |
20120135398 | Method of Detection and Related Detection Device - A method that uses an (DOPC) surfactant based biofilm that reacts with a material in a known manner, and a device that utilizes such a biofilm, to detect a material of interest is provided. The principles of the present invention are particularly useful in detecting/measuring a material that is harmful to a human or to property. | 05-31-2012 |
20120141976 | METHOD FOR DETECTING VIABLE CELLS IN A SAMPLE BY USING A VIRUS - A method for detecting viable cells such as bacterial cells, within a sample, said method comprising (i) incubating said sample with a virus which is able to infect said cells under conditions which allow said virus to infect and replicate within any such cells which are viable; and (ii) detecting any nucleic acid obtained by replication of the virus in said cell. | 06-07-2012 |
20120141977 | HEPATITIS B VIRUS MUTATION STRAIN WITH RESISTANCE TO ADEFOVIR DIPIVOXIL AND THE USES THEREOF - A HBV mutation strain is provided, wherein rtE218G mutation occurs at the polymerase region of the mutation strain. The use of the HBV strain in screening anti-HBV drugs and related detection reagents used to detect rtE218G mutation are also provided. | 06-07-2012 |
20120141978 | PRRSV GP5 BASED COMPOSITIONS AND METHODS - The disclosure includes compositions and methods for the production of an immune response against porcine reproductive and respiratory syndrome (PRRS) virus, or PRRSV. The disclosure is based in part on the use of two or more peptide domains, each with a different sequence, from the PRRSV GP5 protein ectodomain. Compositions and methods comprising polypeptides containing the two or more domains, or nucleic acids encoding them, are described. | 06-07-2012 |
20120141979 | OLIGONUCLEOTIDES FOR DETECTING CHICKEN ASTROVIRUS - There is provided an oligonucleotide sequence capable of binding to a portion of a CAstV genome, wherein the oligonucleotide sequence has binding specificity to the precapsid region of CAstV or to cDNA of the precapsid region. The oligonucleotide sequence can be one of a primer pair for use in a method for detecting the presence of CAstV in a biological sample by reverse transcription followed by amplification of the reverse transcription products using such primer pair, or a method for amplifying CAstV cDNA using such primer pair. | 06-07-2012 |
20120141980 | METHOD FOR ISOLATING VIRUSES - The present invention relates to a method and kit for the isolation of viruses from a sample. The sample is treated with an extraction solution that comprises at least a divalent chloride salt and/or an ionic liquid. | 06-07-2012 |
20120149006 | CELLULOSE SUBSTRATES, COMPOSITIONS AND METHODS FOR STORAGE AND ANALYSIS OF BIOLOGICAL MATERIALS - The invention provides a method and article for storing genetic material or analytes from a biological sample by contacting said biological sample with a cellulose substrate comprising structural units of Formula I | 06-14-2012 |
20120149007 | METHOD FOR DETECTING ANALYTES - The invention provides an improved method for sensitive and specific detection of target molecules, cells, or viruses. The inventive method uses large area imaging to detect individual labeled targets complexed with a target-specific selection moiety. The invention eliminates wash steps through the use of target-specific selection through one or more liquid layers that can contain optical dye and density agents. By eliminating washes the invention simplifies instrumentation engineering and minimizes user steps and costs. The invention uses sensitive image analysis to enumerate individual targets in a large area, is scalable, and can be deployed in systems ranging in complexity from manual to highly automated. | 06-14-2012 |
20120149008 | CHROMATOGRAPHIC SYSTEM FOR NUCLEIC ACID DETECTION - The present disclosure provides a strip for separation and sequence specific detection of nucleic acids in a sample, a system for sequence specific detection of nucleic acids comprising the strip of the present disclosure and an epifluorescence detection device, and a method for qualitative and/or quantitative determination of a nucleic acid using the strip. The strip, system and method of the present disclosure is easy to use and provides accurate and reliable results due to its high sensitivity and specificity in a relatively short analysis time compared to the conventional assays. | 06-14-2012 |
20120149009 | VAPORIZATION DEVICE AND METHOD FOR IMAGING MASS SPECTROMETRY - Methods and apparatus for analyzing samples are disclosed. The samples are analyzed by vaporizing molecules from a sample in a sample area with a femtosecond laser beam under ambient conditions, ionizing the vaporized molecules with electrospray ionization under the ambient conditions to form ions; and analyzing and detecting the ions. | 06-14-2012 |
20120149010 | BACULOVIRUS-BASED PRODUCTION OF BIOPHARMACEUTICALS FREE OF CONTAMINATING BACULOVIRAL VIRIONS - The present invention relates to methods for the production of biopharmaceuticals implementing a baculovirus-based system. These methods advantageously allow the production of biopharmaceuticals with reduced or no contaminating baculoviral virions. | 06-14-2012 |
20120149011 | METHOD FOR DETECTING TARGET NUCLEIC ACIDS - The invention relates to a method for detecting target nucleic acids which are detected by means of a specific sequence tag which is not part of the target nucleic acid. | 06-14-2012 |
20120156670 | IN VITRO UROGENITAL CO-CULTURE MODELS - The invention is directed to co-culture systems comprising (i) rotating wall vessel (RWV)-cultured epithelial or differentiated tissue attached to microcarrier beads and (ii) the peripheral tissue equivalent (PTE) module of the MIMIC® system, and to methods of using the co-culture systems for assessing chemical or biological (bacterial or viral) insults. The system models mucosal exposure to chemicals, pathogens or antigen at various sites in the human body. The microcarrier and MIMIC® co-culture approach provides an in vitro co-culture model that simultaneously demonstrates mucosa-mediated antigen presentation and immunogenic responses. Models of the present invention can be used, for example, in assessments of disease pathogenesis and in pharmaceutical development, reproductive physiology, and immunological and toxicological evaluations. Models of the present invention can generate patient-specific localized mucosal immunology using primary cells, resembling the human physiological situation. | 06-21-2012 |
20120164624 | SERS Nanotag Assays - Methods and systems for the use of Surface Enhanced Raman Scattering nanotags (SERS nanotags) to create homogeneous (no-wash), heterogeneous or sequence detection assay platforms. In certain embodiments the SERS nanotags are used in combination with magnetic particles. Multiplexed assay platforms are also disclosed. In certain embodiments, the assay is useful for clinical proteomics. Assay platforms suitable for use within a biological matrix, for example within whole blood or serum are also disclosed. The assay formats described herein may be used to detect any analyte of interest including but not limited to the detection of cells, viruses, bacteria, proteins, DNA, RNA, or small molecules in any type of biological (animal or plant kingdom) or environmental samples including but not limited to whole blood or serum, occult samples, urine, feces, air, drinking water, phage, any organism, multicellular clumps of cells, for example, cancer tissue homogenate. | 06-28-2012 |
20120164625 | METHODS FOR RAPID IDENTIFICATION OF PATHOGENS IN HUMANS AND ANIMALS - The present invention provides methods of: identifying pathogens in biological samples from humans and animals, resolving a plurality of etiologic agents present in samples obtained from humans and animals, determining detailed genetic information about such pathogens or etiologic agents, and rapid detection and identification of bioagents from environmental, clinical or other samples. | 06-28-2012 |
20120164626 | DETECTION OF HIGH GRADE DYSPLASIA IN CERVICAL CELLS - Methods of using probes and probe sets for the detection of high grade dysplasia and carcinoma in cervical cells are described. Methods of the invention include hybridizing one or more chromosomal probes to a biological sample obtained from a subject and detecting the hybridization pattern of the chromosomal probes to the sample to determine whether the subject has high grade dysplasia or carcinoma. Methods of the invention also include preliminary screening the cells for a marker associated with a risk for cancer, and preferably involves screening for HPV infected cells by in situ hybridization using an HPV probe mixture. | 06-28-2012 |
20120164627 | METHODS AND DEVICES FOR MICROFLUIDIC POINT-OF-CARE IMMUNOASSAYS - Microfluidic methods and devices for heterogeneous binding and agglutination assays are disclosed, with improvements relating to mixing and to reagent and sample manipulation in systems designed for safe handling of clinical test samples. | 06-28-2012 |
20120164628 | AFFINITY CAPTURE OF CIRCULATING BIOMARKERS - Methods, devices and systems for capturing biomarkers are provided. In particular, methods, compositions, and systems that utilize affinity capture devices comprising a processing chamber, affinity capture agent and porous membrane are provided. | 06-28-2012 |
20120164629 | Antibodies For Oncogenic Strains Of HPV And Methods Of Their Use - The subject invention provides antibodies, including polyclonal and monoclonal antibodies, that bind to E6 proteins from at least three oncogenic strains of HPV. In general, the antibodies bind to amino acids motifs that are conserved between the E6 proteins of different HPV strains, particularly HPV strains 16 and 18. The subject antibodies may be used to detect HPV E6 protein in a sample, and, accordingly, the antibodies find use in a variety of diagnostic applications, including methods of diagnosing cancer. Kits for performing the subject methods and containing the subject antibodies are also provided. | 06-28-2012 |
20120171660 | METHOD FOR SCREENING AND PURIFYING ENTEROVIRUS, METHOD FOR MASS-PRODUCING ENTEROVIRUS, AND METHOD FOR MANUFACTURING ENTEROVIRUS VACCINE - The present invention relates to methods for screening or purifying enteroviruses, a method for mass-producing enteroviruses, and a method for manufacturing an enterovirus vaccine. The method for screening enteroviruses in a sample comprises the following steps: (A) providing a sample and a carrier, wherein monosaccharides such as glucose or galactose are bound to the surface of the carrier, and the monosaccharides have binding affinity to enterovirus; (B) contacting the sample with the carrier; (C) removing components of the sample that do not bind to the carrier; (D) providing a detection unit and contacting the detection unit with the carrier, wherein the detection unit binds to the sample bound on the carrier; and (E) measuring a signal of the detection unit, wherein when the signal of the detection unit is detected, it represents that the enterovirus exists in the sample. | 07-05-2012 |
20120171661 | Detection of Antigens - The invention discloses a method for detecting at least one antigen, comprising the following steps: providing magnetic beads, which are coated with antibodies specific for at least one antigen to be detected; bringing the magnetic beads in contact with a washing buffer that comprises at least 8% BSA and incubating the mixture with a sample; isolating the magnetic beads by means of a magnetic separator; and detecting the antigens bound to the magnetic beads by the antibodies. Washing buffers, primers, kits and devices that can be used for said method are also disclosed. | 07-05-2012 |
20120171662 | Simplified Device for Nucleic Acid Amplification and Method for Using Same - The present invention relates to a disposable device ( | 07-05-2012 |
20120171663 | System for Detection of Occult Murine Cytomegalovirus (MCMV) - Methods and compositions for detecting, treating, characterizing, and diagnosing cytomegalovirus in mammals using a nested-PCR methodology using specific predesigned primers are described. | 07-05-2012 |
20120171664 | METHODS AND DEVICES FOR DETECTION OF THE STRAIN OF A PATHOGEN - Provided are methods and devices for determining the strain of a pathogen in a sample. | 07-05-2012 |
20120178078 | Methods and Compositions for Nucleic Acid Detection - The present application relates to Multicomponent Nucleic Acid Enzymes (MNAzymes), which may be used for detecting, identifying and/or quantifying targets. More particularly, this application provides methods of designing and making more reliable MNAzymes, as well as compositions comprising MNAzyme components and methods of using MNAzymes. | 07-12-2012 |
20120178079 | Human Bocavirus and Methods of Diagnosis and Treatment - Human parvovirus, genus | 07-12-2012 |
20120183949 | Method, device, or system using lung sensor for detecting a physiological condition in a vertebrate subject - Devices, systems, and methods are disclosed herein for detecting one or more physiological conditions in lungs of a vertebrate subject. A method is described for administering at least one microparticle to lungs of a vertebrate subject, wherein the at least one microparticle includes one or more markers; wherein the one or more markers is configured to be released in response to one or more physiological conditions in the vertebrate subject; and detecting the one or more markers in a lung exhalant of the vertebrate subject. | 07-19-2012 |
20120183950 | TISSUE ANALYSIS - The present invention provides a method of tissue analysis, disease modelling or drug development comprising the steps of providing a tissue sample comprising cells of at least two different cell types; exposing cells in the tissue sample to an agent ex vivo; exposing the cells to three or more detection means arranged to distinguish between three or more different cell types and/or different cell states; detecting the detection means in order to determine the different cell types and/or different cell states in the sample. The invention further provides a kit for performing the method of the invention and uses of the method of the invention. | 07-19-2012 |
20120183951 | COMPOSITIONS FOR USE IN IDENTIFICATION OF ARENAVIRUSES - The present invention relates generally to the detection and identification of arenaviruses and provides methods, compositions and kits useful for this purpose when combined, for example, with molecular mass or base composition analysis. | 07-19-2012 |
20120183952 | COMPOSITIONS FOR USE IN IDENTIFICATION OF CALICIVIRUSES - The present invention relates generally to the detection and identification of caliciviruses and provides methods, compositions and kits useful for this purpose when combined, for example, with molecular mass or base composition analysis. | 07-19-2012 |
20120190006 | OPTOELECTRONIC DETECTION SYSTEM - The invention relates to optoelectronic systems for detecting one or more target particles. The system includes a reaction chamber, a specimen collector, an optical detector, and a reservoir containing cells, each of the cells having receptors which are present on the surface of each cell and are specific for the target particle to be detected, where binding of the target particle to the receptors directly or indirectly activates a reporter molecule, thereby producing a measurable optical signal. | 07-26-2012 |
20120190007 | METHOD AND SYSTEM FOR MONITORING AND RECORDING A VIRAL INFECTION PROCESS AND THAT FOR SCREENING VACCINES - The present invention relates to a method and system for monitoring and recording a viral infection process and that for screening vaccines, which is characterized by providing a microcantilever detection device, which comprises a microcantilever comprising a contact area having an macromolecular material attached thereon; allowing host cell to be attached to the macromolecular material; allowing a sample containing a test virus or vaccine to be loaded into the contact area to make the test virus or vaccine to contact the host cells attached to the macromolecular material so as to produce a deflection of the microcantilever; and recording the deflection in a time course manner so as to obtain a deflection profile that can used as a basis for determining the viral infection process or the potential efficacy of the vaccines. | 07-26-2012 |
20120190008 | GENERIC PCR - The present invention provides a method for the amplification of at least a first and a second target nucleic acid that may be present in a fluid sample. The invention further provides a kit and an analytical system for carrying out said amplification. | 07-26-2012 |
20120190009 | Viral Modulators and Processes Thereof - A viral modulator and process thereof. A method may include contacting one or more viral modulators to one or more biological systems. A biological system may be configured to be infected by one or more virus. A virus may include an HIV virus, a VEEV virus and/or the like. A viral modulator may include a viral inhibitor and/or a viral activator. | 07-26-2012 |
20120190010 | Generic Matrix for Control Nucleic Acids - The present invention belongs to the field of in-vitro diagnostics. Within this field, it particularly concerns the amplification of at least a first target nucleic acid that may be present in at least one fluid sample using an internal control nucleic acid for qualitative and/or quantitative purposes and at least one external control nucleic acid in an aqueous buffer. It further provides an analytical system comprising an internal control nucleic acid for qualitative and/or quantitative purposes and at least one external control nucleic acid in an aqueous buffer. | 07-26-2012 |
20120190011 | COMPOSITIONS AND METHODS FOR MAMMALIAN GENETICS AND USES THEREOF - The invention provides compositions and methods for performing mammalian cell genetics, e.g., genetic screens, using near-haploid cells. The invention further provides genes and gene products isolated using the inventive methods and methods of use thereof. | 07-26-2012 |
20120196272 | Prediction of HCV Viral Kinetics in Interferon-Free Treatment - The present invention is based on the discovery of associations that exist between single nucleotide polymorphisms (SNPs) on chromosome 19 and virological outcomes in a diverse population of patients with hepatitis C virus (HCV) who received interferon-free treatment. | 08-02-2012 |
20120196273 | DEVICES AND METHOD FOR ENRICHMENT AND ALTERATION OF CELLS AND OTHER PARTICLES - The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample. | 08-02-2012 |
20120196274 | MATERIALS AND METHODS FOR GENOTYPING AND QUANTIFYING A HIGH-RISK HUMAN PAPILLOMAVIRUS - Nucleic acids, assays, and methods for the detection and quantification of high risk HPV types are disclosed. | 08-02-2012 |
20120196275 | MULTICELLULAR ORGANOTYPIC MODEL OF HUMAN INTESTINAL MUCOSA - Disclosed are methods of preparing multi-cellular three-dimensional tissue constructs, that include fibroblasts, endothelial cells, lymphocytes and epithelial cells. The present methods may include embedding fibroblasts and endothelial cells in a matrix enriched with gut basement membrane proteins to form a cell containing matrix that is then added to a bioreactor and exposed to epithelial cells and activated lymphocytes as the cell cultures. Also provided are the tissue constructs formed from such methods, a matrix enriched with gut basement membrane proteins and kits that include the same. Further provided are methods of measuring toxicity of a pathogen or commensal organisms, chemosensitivity of tissues to a toxic material and inflammatory conditions, which use the present multi-cellular three-dimensional tissue constructs. | 08-02-2012 |
20120196276 | Mixed Cell Diagnostic Systems For Detection Of Respiratory, Herpes and Enteric Viruses - The present invention generally relates to the field of diagnostic microbiology, and, more particularly, to compositions and methods for detecting and differentiating one or more viruses or other intracellular parasites present in a specimen. The present invention also provides compositions and methods to evaluate the susceptibility of organisms to antimicrobial agents. | 08-02-2012 |
20120196277 | DIAGNOSIS OF LIVER PATHOLOGY THROUGH ASSESSMENT OF PROTEIN GLYCOSYLATION - Methods for diagnosing pathology of the liver in a subject suspected of having such pathology are disclosed. The methods comprise quantifiably detecting lectin binding on proteins in biological fluids, and comparing the detected lectin binding with reference values for the binding of lectin of such proteins in healthy or disease states. | 08-02-2012 |
20120202189 | RAPID, SEMI-AUTOMATED METHOD TO DETECT RESPIRATORY VIRUS INFECTED CELLS IN DIRECT SPECIMENS - A novel cytometer system, methods and algorithms which provide a rugged, affordable and easy-to-use device in remote locations. All cells in a biological sample are fluorescently labeled, with target cells also magnetically labeled. Non-magnetically labeled cells are imaged for viability. Labeled sample is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells for observation. A LED illuminates the cells and a CCO camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a cell count that can be related to the target cell concentration of the original sample. | 08-09-2012 |
20120202190 | DETECTION OF HEPATITIS B VIRUS IN BLOOD USING LAMP METHOD - A method for detection blood borne pathogen in whole blood, comprising collecting a blood sample from a subject, heating said sample for a period of time, adding said heated sample to a pre-mixed LAMP solution comprising one or more LAMP primer set, and Bst DNA polymerase to create a reaction mixture, incubating said reaction mixture for a period of time and determining the presence of blood born pathogen. | 08-09-2012 |
20120202191 | DETECTION METHOD BASED ON TIME RESOLVED REAL TIME FLUORESCENT ENERGY TRANSFER (TR-FRET) - A method for detecting the presence of a diagnostic moiety indicative of exposure to an infectious organism in a biological sample taken from a human or animal, said method comprising use of a first and second fluorescently labelled reagent which are capable of binding to a diagnostic moiety or to a binding partner in competition with a diagnostic moiety, wherein labels on the first and second labelled reagents act as fluorescent donors and acceptors to one another, the proximity of the reagents to one another being detectable by measuring the emission of fluorescent energy from at least one of the labels. | 08-09-2012 |
20120202192 | HYPER-SPECTRAL IMAGING AND ANALYSIS OF A SAMPLE OF MATTER, FOR IDENTIFYING AND CHARACTERIZING AN OBJECT OF INTEREST THEREIN - Method for hyper-spectral imaging and analysis of a sample of matter, for identifying and characterizing an object of interest therein. Preparing test solution or suspension of the sample, including adding thereto a spectral marker specific to object of interest, such that if object of interest is in test solution or suspension, object of interest becomes a hyper-spectrally active target which is hyper-spectrally detectable and identifiable; adding to test solution or suspension a background reducing chemical, for reducing background interfering effects caused by presence of objects of non-interest in test solution or suspension, thereby increasing hyper-spectral detectability of hyper-spectrally active target in test solution or suspension; generating and collecting hyper-spectral image data and information of test solution or suspension; and, processing and analyzing thereof. Exemplary objects of interest are biological agents—bacteria ( | 08-09-2012 |
20120202193 | DISPOSABLE DEVICE FOR STORING BIOLOGICAL LIQUIDS AND USE THEREOF TO DETECT MATERIALS, PARTICLES, AND/OR CELLS - The invention relates to a disposable device, in which the target substances are bound to solid carriers, preferably microparticles. A mechanism according to the invention enables the solid carriers to be easily concentrated to a volume of appropriate size. A further device according to the invention makes it possible for the solid carriers in the device to be subjected to known isolating, amplifying and/or detection reactions. The device is especially suitable for the enrichment and isolation and/or detection of substances in, or extracted from, biological liquids. | 08-09-2012 |
20120202194 | SUBSTANCE DETERMINING APPARATUS - The invention relates to a substance determining apparatus for determining a substance within a fluid. Particles, which have attached the substance, are bound to a binding surface ( | 08-09-2012 |
20120208174 | Plasmonic System for Detecting Binding of Biological Molecules - Detection and characterization of molecular interactions on membrane surfaces is important to biological and pharmacological research. In one embodiment, silver nanocubes interfaced with glass-supported model membranes form a label-free sensor that measures protein binding to the membrane. The present device and technique utilizes plasmon resonance scattering of nanoparticles, which are chemically coupled to the membrane. In contrast to other plasmonic sensing techniques, this method features simple, solution-based device fabrication and readout. Static and dynamic protein/membrane binding are monitored and quantified. | 08-16-2012 |
20120208175 | DETECTION OF HIV-RELATED PROTEINS IN URINE - A method for detecting HIV infection in a mammal is disclosed. The method contains the steps of isolating exosomes from a urine sample of a mammal and detecting the presence of HIV-specific biomarker in said isolated exosomes. A method for diagnosing a mammal with an HIV-associated disease, in particular, HIV-associated nephropathy is also disclosed. | 08-16-2012 |
20120208176 | Multivalent Epitope in Complex with a Detection Marker for the Early Serodiagnosis of Infections - The present invention relates to a new method to determine the presence of antibodies to a pathogen in a serological sample using a new detection reagent which comprises at least two and preferably at least four copies of an antigen from the pathogen and a detectable marker. The present invention also relates to a new detection reagent which consists of at least two and preferably at least four antigenic peptides in a complex with a detectable marker via interacting multimerization domains upon both the antigenic peptides and detectable marker. | 08-16-2012 |
20120208177 | ASSAY METHODS FOR MDV-1 - A method for the quantification of a vaccine strain and/or a virulent strain of Wlarek's Disease Virus Serotype-1 (MDV-1) in a sample from a bird, comprising the steps of: (i) providing a biological sample from the bird and optionally isolating nucleic acid from the biological sample; (ii) subjecting the biological sample of (i) to real-time quantitative PCR (qPCR) comprising: (a) amplification of a region of the pp3B gene within the nucleic acid sample of (i), said region containing a consistent single nucleotide polymorphism (SNP) difference between vaccine and virulent strains of MDV-1; and (b) contacting the amplified nucleic acid of (a) with a detectable nucleic acid probe specific for the SNP of the vaccine strain of MDV-1 and/or a detectable nucleic aα′d probe specific for the SNP of the virulent strain of MDV-1; and (iii) Measuring changes in the detectable signal produced by the probe of (ii). Methods are also provided for the absolute quantification of vaccine and virulent viruses. | 08-16-2012 |
20120208178 | PROBES COMPRISING FLUORESCENT ARTIFICIAL NUCLEOBASES AND USE THEREOF FOR DETECTION OF SINGLE BASE ALTERATION - The present invention relates to forced intercalation probes (FIT-probes) comprising at least one nucleoside analogue which comprises at least a fluorescent artificial nucleobase directly bound to a carbon of a modified sugar moiety wherein said modified sugar moiety is a carba-sugar or an amino acid nucleic acid (AANA). Thereby the nucleoside analogue is incorporated into DNA or RNA in the place of a single native base. | 08-16-2012 |
20120208179 | COMPOSITIONS FOR USE IN IDENTIFICATION OF ORTHOPOXVIRUSES - Oligonucleotide primers and compositions and kits containing the same for rapid identification of orthopoxviruses by amplification of a segment of viral nucleic acid followed by molecular mass analysis are provided. | 08-16-2012 |
20120208180 | METHOD FOR EARLY DIAGNOSIS OF CARCINOMAS OF THE ANOGENITAL TRACT - A method for the early diagnosis of carcinomas of the anogenital tract, preferably of the cervical carcinoma, and the preliminary stages thereof is described. The method is based on the determination of the methylation status of segments of the gene regions of ASTN1 (astrotactin 1) and ZNF671 (zinc finger protein 671, transcription factor). A DNA methylation in the promoter region or the 5′-region of these genes is indicative for carcinomas of the anogenital tract or severe preliminary stages thereof. The present invention also relates to kits which permit the conduction of the diagnostic method according to the invention, | 08-16-2012 |
20120214151 | DETECTION OF HIV-RELATED PROTEINS IN URINE - A method for detecting HIV infection in a mammal is disclosed. The method contains the steps of isolating exosomes from a urine sample of a mammal and detecting the presence of HIV-specific biomarker in said isolated exosomes. A method for diagnosing a mammal with an HIV-associated disease, in particular, HIV-associated nephropathy is also disclosed. | 08-23-2012 |
20120214152 | RNASCOPE.RTM. HPV ASSAY FOR DETERMINING HPV STATUS IN HEAD AND NECK CANCERS AND CERVICAL LESIONS - The present invention provides a method and a kit for determining whether a head and neck cancer is HPV-related. In one embodiment, an RNAscope® HPV assay was designed to detect the presence of E6/E7 mRNA of certain high-risk HPV subtypes related to head and neck cancer. The present invention also provides a method and a kit for determining whether a cervical lesion is a benign lesion or a cervical intraepithethial neoplasm lesion. The present invention further provides a method for determining the progression of cervical intraepithethial neoplasm based on the spatial pattern and levels of the E6/E7 mRNA of certain high-risk HPV subtypes. The present invention also provides a method for determining the risk of developing cervical cancer in a human diagnosed with cervical intraepithethial neoplasm based on presence and absence of the certain subgroups of high-risk HPV subtypes. | 08-23-2012 |
20120214153 | Detection of an Analyte in a Sample - There is provided mechanisms for the detection of an analyte in a sample. The mechanisms utilize at least a first measurement channel comprising a detection reactant corresponding to the analyte to be detected, and at least a microstructure associated with the first measurement channel. When the mechanisms are in use, the sample is introduced into the first measurement channel and propagated by way of the first measurement channel towards the microstructure. If the analyte is present in the sample, the analyte interacts with the detection reactant to form a networked product, and the microstructure is configured to filter the networked product. | 08-23-2012 |
20120214154 | GENETICALLY ENGINEERED BIOLOGICAL INDICATOR - The disclosed technology relates to a genetically engineered biological indicator, comprising: at least one test organism and at least one reporter gene suitable for producing an indicator enzyme, the reporter gene being taken up by the test organism; and at least one repressor gene that inhibits expression of the reporter gene until the reporter gene is exposed to at least one inducer. A process and an apparatus for using the biological indicator are disclosed. | 08-23-2012 |
20120214155 | DIAGNOSTIC ASSAYS FOR CHORDOPOXVIRUSES - This disclosure relates to compositions and methods of their use in detection and identification of a chordopoxvirus in a sample, such as diagnosis of an infection in a subject. The compositions and methods allow for detection and identification of all non-avian low-GC content chordopoxviruses, identification of most known high-GC content chordopoxvirus, and species-specific detection of Canarypox virus, Fowlpox virus, and Sealpox virus. | 08-23-2012 |
20120219941 | Identification of Oligonucleotides for the Capture, Detection and Quantitation of Hepatitis A Viral Nucleic Acid - Hepatitis A virus-specific primers and probes derived from conserved regions of the hepatitis A virus genome are disclosed. Also disclosed are nucleic acid-based assays using the capture oligonucleotides, primers and probes. | 08-30-2012 |
20120225421 | KIT AND METHOD FOR SEQUENCING A TARGET DNA IN A MIXED POPULATION - Methods and kits for sequencing a target DNA sequence in a sample having a related reference sequence are provided herein. In particular, kits and methods for sequencing cancer and cancer therapy associated mutations are described. Also provided are kits and methods for detecting mitochondrial mutations and for differentiating between closely related viral strains. | 09-06-2012 |
20120225422 | METHOD AND DEVICE EMPLOYING A NON-RECEPTOR LIGAND INTERACTION WITH NANOPARTICLES OR OTHER SOLID PHASE FOLLOWED BY SPECIFIC DETECTION - A method and apparatus for conducting specific binding assays are claimed. This invention relates to an initial non-receptor ligand interaction with nanoparticles or other solid phases followed by specific detection methods used for detecting biological, chemical or environmental entities. This invention employs an on-device ligand attachment to nanoparticles or other solid phase followed by the specific capture in discrete zones on the device. The ligand-bound complexes assemble on the ligand specific capture zone leading to a visible diagnostic result, if colored solid phases are employed. | 09-06-2012 |
20120225423 | PATHOGEN DETECTION BIOSENSOR - The invention described herein provides methods for the detection of target particles, such as pathogens, soluble antigens, nucleic acids, toxins, chemicals, plant pathogens, blood borne pathogens, bacteria, viruses and the like. Also described is an emittor cell comprising a receptor, wherein the receptor can be an antibody or an Fc receptor, and an emittor molecule for the detection of a target particle in a sample wherein the target particle to be detected is bound by one or more receptors on the emittor cell. Also provided are optoelectronic sensor devices for detecting a target particle in a sample, including in a plurality of samples. | 09-06-2012 |
20120225424 | Device and Method for Electroporation-Based Delivery of Molecules Into Cells and Dynamic Monitoring of Cell Responses - The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms. | 09-06-2012 |
20120231444 | Microfabricated Crossflow Devices and Methods - This invention provides microfabricated devices and methods for detecting, analyzing and sorting biological materials and particles. Droplets containing the particles are provided in an extrusion fluid, passed through a detection region, and then directed into a branch channel according to predetermined characteristics. For example, cells or viral particles contained in droplets of aqueous solvent are flowed past a detector in the nonpolar extrusion fluid decane, and routed into a selected branch channel for subsequent analysis or use. | 09-13-2012 |
20120231445 | OLIGONUCLEOTIDES AND PROCESS FOR DETECTION OF SWINE FLU VIRUS - A rapid, sensitive and cost effective process of detection of swine flu virus H1 type in a sample is provided herein. The present invention further provides highly specific oligonucleotides useful for rapid detection of swine flu virus in a sample. Swine flu virus specific isothermal gene amplification assay disclosed in the present invention is highly specific and sensitive and is useful for early clinical diagnosis of H1N1 human patients. The oligonucleotides disclosed in the present invention provide amplification of the target gene in highly sensitive and specific manner showing no cross amplification. | 09-13-2012 |
20120231446 | METHOD FOR SELECTIVELY ENRICHING AND ISOLATING MICROBIAL AND OPTIONALLY ADDITIONAL VIRAL NUCLEIC ACIDS - The present invention relates to a method for selectively enriching and/or isolating microbial and optionally additionally viral nucleic acids from samples that contain a mixture of microbial cells, freely circulating nucleic acids and higher eukaryotic cells, and optionally additionally viruses, in a liquid, and to a kit for selectively enriching and/or isolating intracellular and extracellular microbial nucleic acids, and optionally additionally viral nucleic acids, from samples that contain a mixture of microbial and higher eukaryotic cells, freely circulating nucleic acids, in particular extracellular microbial nucleic acids, and optionally additionally viruses in a liquid. | 09-13-2012 |
20120237921 | FULL GENOME DNA OF HUMAN CYTOMEGALOVIRUS STRAIN JHC ISOLATED FROM KOREAN PATIENT AND OPEN READING FRAMES THEREOF - Provided are a full genome DNA of a human cytomegalovirus (HCMV) strain JHC isolated from Korean patients and open reading frames (ORFS) thereof and, more particularly, UL1, UL119 and RL6. | 09-20-2012 |
20120237922 | Viral Diagnostics - The present disclosure provides methods for determining whether a subject is infected with lymphocytic choriomeningitis virus (LCMV). These methods include obtaining a sample from a subject with increased susceptibility to LCMV infection, contacting the sample with one or more compositions for detecting LCMV, and determining whether the one or more compositions for detecting LCMV is associated with a marker of LCMV from the sample, wherein detection of an association indicates that that the subject is infected with LCMV. | 09-20-2012 |
20120237923 | Lateral Flow Immunoassay Controls - Rapid lateral flow immunoassays have an extensive history of use in both the clinical and home settings. These devices are used to test for a variety of analytes, such as drugs of abuse, hormones, proteins, urine or plasma components and the like. The present invention provides an improved procedural control that indicates to the test user that at least a portion of the applied sample has passed through the test result zone of the test strip, and optionally that the test is complete and the test results may be read. | 09-20-2012 |
20120244521 | Methods for identifying a virulent strain of a virus - The present invention relates to methods for identifying a virulent strain of a virus, particularly and influenza virus, by detecting specific mutations in the amino acid sequence of the hemagglutinin (HA) protein and by determining the case fatality rate for hospitalization (CFR/H) as the number of persons hospitalized for infection by the virus who die from the infection compared to the total number of persons hospitalized for infection, wherein the identification of mutations in the HA protean and/or an increasing CFR/H over time indicates a virulent strain of the virus. | 09-27-2012 |
20120244522 | COMPOSITIONS FOR USE IN IDENTIFICATION OF ALPHAVIRUSES - The present invention provides oligonucleotide primers and compositions and kits containing the same for rapid identification of alphaviruses by amplification of a segment of viral nucleic acid followed by molecular mass analysis. | 09-27-2012 |
20120244523 | System and Method for Detection of HIV Integrase Variants - An embodiment of a method for detecting low frequency occurrence of one or more HIV sequence variants associated with integrase is described that comprises the steps of: (a) generating a cDNA species from a plurality of RNA molecules in an HIV sample population; (b) amplifying a plurality of first amplicons from the cDNA species, wherein each first amplicon is amplified with a pair of nucleic acid primers capable of amplifying products from clades A, B, C, D, AE and G sub-types; (c) clonally amplifying the amplified copies of the first amplicons to produce a plurality of second amplicons; (d) determining a nucleic acid sequence composition of the second amplicons; (e) detecting one or more sequence variants that occur at a frequency of 5% or less in the nucleic acid sequence composition of the second amplicons; and (f) correlating the detected sequence variants with variation associated with HIV integrase. | 09-27-2012 |
20120244524 | METHOD FOR COUNTING AND SEGMENTING VIRAL PARTICLES IN AN IMAGE - The method is for intracellular counting and segmentation of viral particles or infectious agents in an image. An image is provided that has a plurality of items therein. A radius range of viral particles is determined. Items in the image having a radius within the predetermined radius range are identified. Elliptical items that are formable from the predetermined radius range are determined. The round and elliptical items identified into groups are sorted. The viral particles among the round and elliptical items are identified. For example, the method may be used for intracellular counting and segmentation of siRNA treated human cytomegaloviral particles in TEM images. | 09-27-2012 |
20120252002 | FLUORESCENT NANOPARTICLE COMPOSITES THEMSELVES, PROCESS FOR THE PREPARATION OF SUCH COMPOSITES, AND USE IN RAPID DIAGNOSIS SYSTEMS WITH AFFINITY TO BIOLOGICAL MOLECULES - The present invention provides fluorescent nanoparticle composites themselves, the process of preparing such composites, to systems for rapid diagnosis (as “kits”) containing such composites, and to the use of such composites. In a preferential embodiment, the composites of the present invention have an affinity for biological molecules, such as DNA. The present invention also comprises the preparation of probes containing biological material, upon which are added fluorescent nanoparticle composites, making viable a rapid and economic biological diagnosis of, for example, diseases and genetic traits, notably in the medical and veterinarian fields. There is yet the fact that the absorption of radiation in the ultraviolet and visible regions, with the emission of light in the near ultraviolet and visible range, including in the colors of deep blue and/or green, provides advantageous use of its fluorescent properties in photovoltaic or electroluminescent devices, such as organic LEDs, or for the increase in luminous gain of fluorescent lamps, which represents another characteristic of the invention. | 10-04-2012 |
20120252003 | Bacteria Identification by Phage Induced Impedance Fluctuation Analysis - Methods for detection and identification of bacteria within a sample include the step of inserting a pair of electrodes into the sample. A first impedance across the electrodes is established with a first AC voltage source having a first frequency. A phage is introduced into the sample, and impedance fluctuations that are caused by ion release by the bacteria due to the phage introduction are measured. The use of impedance fluctuations instead of voltage fluctuations to detect and identify bacteria minimizes 1/f noise effects and increases system sensitivity. To further increase system sensitivity by eliminating thermal noise, a second impedance across the electrodes can be established using a second AC voltage source at a second frequency. Second impedance fluctuations are cross-correlated to the first impedance fluctuations, and the cross-correlation results are analyzed to determine whether or not bacteria are present in the sample based on phage electrical activity. | 10-04-2012 |
20120252004 | HIGHLY SENSITIVE IMMUNOCHROMATOGRAPHY METHOD - Provided is an immunochromatography method that enables highly sensitive detection by reducing the background density of a non-measurement site in the immunochromatography method. The immunochromatography method includes developing a complex of a substance to be tested and a labeling substance containing a metal modified with a first binding substance for the substance to be tested on an insoluble carrier in the presence of a surfactant having a steroid skeleton while the substance to be tested and the labeling substance form the complex; and detecting the complex of the substance to be tested by capturing the substance to be tested and the labeling substance on a detection site of the insoluble carrier containing a second binding substance for the substance to be tested or a substance that can bind to the first binding substance for the substance to be tested. | 10-04-2012 |
20120252005 | MULTI-WAVELENGTH ANALYSES OF SOL-PARTICLE SPECIFIC BINDING ASSAYS - The present invention discloses methods for detecting the presence of a complex between a first reagent and a second reagent in solution. In particular, the invention provides methods for qualitative or quantitative detection of an analyte or its specific binding partner in complex biological samples. The invention further discloses algorithms using summary rate changes at selected wavelengths in the absorbance spectra of colloidal metal-labeled analytes or specific binding partners to identify intermolecular interactions between the analyte and its binding partner. | 10-04-2012 |
20120252006 | Methods and Systems for Multiple Control Validation - The invention provides methods for validating a multiplex binding assay that results in a reduced number of false invalidations. The invention further provides systems for validating a multiplex binding assay that results in a reduced number of false invalidations. The invention further provides a computer readable medium containing program instructions for validating a multiplex binding assay that results in a reduced number of false invalidations. | 10-04-2012 |
20120252007 | OPTIMIZED REAL TIME NUCLEIC ACID DETECTION PROCESSES - This invention provides for compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided. Paneling and multiplex analyses of more than one nucleic acid analyte using one sample are also provided. | 10-04-2012 |
20120258442 | DETERMINING TUMOR ORIGIN - The disclosure provides methods for the use of gene expression measurements to classify or identify among 54 cancer types in samples obtained from a subject in a clinical setting, such as in cases of formalin fixed, paraffin embedded (FFPE) samples. | 10-11-2012 |
20120258443 | METHODS FOR THE DETECTION OF JC POLYOMA VIRUS - Methods and compositions for determining whether a subject is at risk for PML, including subjects being treated with immunosuppressants, by determining whether the subject harbors a JCV variant with reduced binding for sialic acid relative to a normal JCV, are presented. Furthermore, combinations of JCV-VP1 sequence variations that are associated with PML and that can be used as a basis of an assay for identifying subjects susceptible to PML, subjects with PML (e.g., early stage PML), or subjects at risk of developing PML in response to an immunosuppressive treatment are provided. | 10-11-2012 |
20120258444 | ACOUSTIC WAVE (AW) SENSING DEVICES USING LIVE CELLS - In one embodiment according to the invention, there is provided a method of sensing a response of a living cell or virus to a change in conditions. The method comprises applying an essentially constant external electromotive force that causes oscillation of an acoustic wave device at essentially constant amplitude and frequency under steady state conditions. The acoustic wave device has attached at least one living cell or virus. A combined oscillating system including the acoustic wave device and the living cell or virus exhibits a fundamental frequency and at least one harmonic frequency of the combined oscillating system. The living cell or virus is exposed to a change in an environmental condition while oscillating the combined oscillating system under the essentially constant external electromotive force, whereby a response of the living cell or virus to the change in environmental condition will be indicated by a change in at least one of frequency and amplitude of the oscillation of at least one harmonic frequency of the combined oscillating system. | 10-11-2012 |
20120258445 | METHODS FOR USING NANOWIRE SENSORS - Methods for using nanowire sensors are described. In one embodiment, the nanowire sensor may be field effect transistor having a nanowire and a functionalized control electrode. One method of using such a nanowire sensor includes exposing the functionalized control electrode to a test sample and an enhancing reagent. In another embodiment, the nanowire sensor may be a field effect transistor having a gate electrode and a functionalized nanowire. One method of using such a nanowire sensor includes exposing the functionalized nanowire to a test sample and an enhancing reagent. The use of an enhancing reagent increases the sensitivity of the nanowire sensor to a substance to be detected or quantified. | 10-11-2012 |
20120258446 | Universal Multi-Variant Detection System - The present invention provides a method to diagnostically detect the variants of a given pathogen, such as HIV, hepatitis C, hepatitis B (HBV), Parvovirus B19, etc., with the use of a single detection probe. | 10-11-2012 |
20120258447 | REAL-TIME MULTIPLEXING DETECTION OF TARGET NUCLEIC ACID SEQUENCES WITH ELIMINATION OF FALSE SIGNALS - The present invention relates to the real-time multiplex detection of at least three target nucleic acid sequences with elimination of false positive signals. Unlikely to conventional real-time multiplex PCR methods, the present invention comprises two different amplification reactions in different reaction vessels from each other: a primary multiplex PCR for obtaining amplicons and a secondary nested real-time multiplex PCR using the amplicons. The present invention permits to eliminate the false positive signals generated by the dimer formation of labeled primers, false positive signals | 10-11-2012 |
20120258448 | MUTATIONAL PROFILE IN HIV-1 GAG CLEAVAGE SITE CORRELATED WITH PHENOTYPIC DRUG RESISTANCE - The invention concerns novel mutations or mutational profiles of HIV-1 protease cleavage sites (CS) in the Gag region correlated with a phenotype causing alterations in sensitivity to anti-HIV drugs. The present invention also relates to the use of genotypic characterization of a target population of HIV and the subsequent association, i.e., correlation, of this information to phenotypic interpretation in order to correlate virus mutational profiles with drug resistance. The invention further relates to methods of utilizing the mutational profiles of the invention in databases, drug development, i.e., drug design, and drug modification, therapy and treatment design and clinical management. | 10-11-2012 |
20120264108 | Intracellular Molecular Delivery Based On Nanostructure Injectors - There is disclosed a method and device for the delivery of molecules into individual cells. A device for injecting a biological molecule into a target cell comprises a microscopic tip attached to a mechanical scanning device for positioning the tip relative to the target cell and for moving the tip into the target cell; a nanostructure, such as a carbon nanotube, fixed on an end of the microscopic tip; and a biological molecule attached to the nanotube by a cleavable electrostatic or chemical linker linking the biomolecule to the nanotube, said chemical linker having a chemical linkage which is cleaved in an intracellular environment. The biological molecule may be one or more of proteins, nucleic acids, small molecule drugs, and optical labels, and combinations thereof. Exemplified are multiple walled carbon nanotubes to which a polycyclic aromatic compound is adsorbed, the aromatic compound having a side chain containing a cleavable disulfide linkage and a biotin functionality for coupling to a streptavidin-linked payload. | 10-18-2012 |
20120264109 | LYMPHOCYTE ANALYSIS FOR MONITORING THE PROGRESSION OF IMMUNODEFICIENCY VIRUS - The present disclosure describes a method of monitoring disease progression in a mammal positive for immunodeficiency virus which includes collecting blood cells from a mammal to obtain a first blood sample adding antibodies such as CD4 and CD8 to the first blood sample scanning the blood sample to produce a first multivariate dot plot which may be used to quantify at least CD4 | 10-18-2012 |
20120264110 | AUTOMATED PAP SCREENING USING A PLURALITY OF BIOMARKERS AND MULTI-SPECTRAL IMAGING - An automated screening method for detecting abnormalities in a sample. The method includes steps of staining a sample with a histologic or cytologic stain for transmission light microscopy to provide a stained sample; exposing the stained sample to a plurality of differentially-labeled biomarkers, wherein each of the biomarkers is labeled with a distinct transmission stain or fluorescence probe; at a first location, generating at least one multi-spectral image of the stained sample using signals obtained from the plurality of differentially-labeled biomarkers and the histologic or cytologic stain; and automatically determining whether the first location requires further pathologist review or interpretation. | 10-18-2012 |
20120264111 | HYDROPHOBIC, FUNCTIONALIZED PARTICLES - The present invention relates to a stable mixture comprising surface-modified particles which are obtained by reacting metal oxide or semimetal oxide particles with at least one compound selected from among silicon-comprising compounds bearing at least one metaloxy radical and optionally further alkoxy and/or hydroxy radical(s) and at least one solvent, at least one surface-active substance or a mixture thereof, a process for producing the mixture, the use of these particles in systems in which they are brought into contact with at least one solvent, where the mass ratio of solvent to modified particle is greater than 500, and also the use of these particles in agglomeration-deagglomeration cycles. | 10-18-2012 |
20120264112 | METHOD OF MEASURING HUMAN CYP3A INDUCIBILITY - A method for measuring human CYP3A inducibility upon administration of a test drug, characterized in that a non-human animal to which a test drug is administered or a population of human cells cultured in a medium containing a test drug is infected with viruses (A) and (B); virus (A) being an adenovirus which is used as a vector and engineered by incorporating thereto a detectable reporter gene and at least 3 human PXR binding regions falling within an untranslated region of a human CYP3A gene, and virus (B) being an adenovirus which is used as a vector and engineered by incorporating thereto a human PXR cDNA; and subsequently expression level of the reporter gene is determined in the non-human animal or the cultured human cells. | 10-18-2012 |
20120264113 | USE OF SUPERHYDROPHOBIC SURFACES FOR LIQUID AGGLUTINATION ASSAYS - This invention relates to the use of thermodynamically incompatible surfaces in agglutination assays for the express purpose of using the sample as a key component of the detection instrument. Specifically, the invention relates to formation of a lense and a virtual container for rapid mixing via thermal energy by a sample liquid disposed on a superhydrophobic surfaces, and a subsequent specific analyte or overall protein concentration assay using particles agglutination for use in the industrial, environmental, and clinical laboratory test fields. | 10-18-2012 |
20120264114 | Disposable Device for the Detection of Particles of Interest, Such as Biological Entities, Detection System Comprising Said Device and Method for Using Same - The invention relates to a disposable device comprising all of the reagents necessary for the detection of one or more particles of interest, and which can be incorporated in a detection system including simple means for the automatic management of fluids. For this purpose, the invention relates to a disposable device for the detection of one or more particles of interest present in a liquid sample, said device comprising a substrate provided with: a chamber for capturing the particle(s) of interest to be detected; a fluid channel connecting, upstream, the capture chamber to a buffer solution container, a liquid sample injection means and a container of labelling probes that can be secured to the particle(s) of interest to be detected; and a fluid channel connecting, downstream, the capture chamber to a container for the recovery of liquids which, during use, can flow from the capture chamber. | 10-18-2012 |
20120270205 | HIGH-FLUX CHEMICAL SENSORS - The present invention relates to the field of chemical detection. Specifically, the invention provides devices that respond quickly to various target chemical analytes present in the environment. Responses are based on a change in an electrical property (such as impedance or resistance) caused by adsorption or absorption of the target analyte(s) to or in a substrate-free chemical sensing element. The chemical sensing element is composed of a thin, electrically conductive polymer material (due to doping of structural polymer material(s) with electrically conductive particles and/or the use of electrically conductive polymer material(s)), which can allow vapors to pass through with little pressure drop. The chemical sensing material is either suspended in the environment, or emplaced adjacent to one or between two porous membranes, resulting in a sensing patch capable of high gas or vapor flux through the chemical sensing element. | 10-25-2012 |
20120270206 | Systems and Methods for Analyzing Nucleic Acid Sequences - The invention relates to systems and methods for analyzing clinically relevant nucleic acid sequences. | 10-25-2012 |
20120270207 | ANALYTE DETECTION - The present disclosure provides methods and/or kits for detecting an analyte in a sample. Some embodiments provide a method for detecting a non-nucleic acid analyte in a sample using a solid substrate comprising a bound immobilisation agent and an antibody capture agent and a detectable agent, which can bind to the analyte. The antibody capture agent comprises, at a plurality of sites, a ligand for the immobilisation agent. A complex between the analyte, the antibody capture agent and a detectable agent is formed and immobilised on the solid substrate by binding between the immobilisation agent and the ligand. In some embodiments, the ligand and the immobilisation agent are a binding pair comprising a peptide tag and an anti-peptide tag antibody. | 10-25-2012 |
20120270208 | SYNTHETIC ANTIGENS FOR THE DETECTION OF ANTIBODIES TO HEPATITIS C VIRUS - Peptide sequences are provided which are capable of mimicking proteins encoded by HCV for use as reagents for screening of blood and blood products for prior exposure to HCV. The peptides are at least 5 amino acids long and can be used in various specific assays for the detection of antibodies to HCV, for the detection of HCV antigens, or as immunogens. | 10-25-2012 |
20120276520 | ASSAY FOR MUTATIONS IN STEM CELLS AND THEIR DERIVATIVES - The present invention provides methods to assess the genetic safety of stem cells, whether endogenous embryonic stem cells, somatic or adult stem cells, or artificially induced stem cells from non-pluripotent cells, and their differentiated derivatives for use in human medicine, and the applications of modified stem cells to testing environmental or potential genetic or epigenetic modulators such as culture media formulations, substrates or scaffolds, additives, reagents, processes, and processing materials used to prepare stem cells for use. | 11-01-2012 |
20120276521 | ORAL FLUID RAPID ASSAY FOR HEPATITIS C VIRUS (HCV) ANTIBODIES USING NON-ANTIBODY LABELING OF lgA MOLECULES RECOGNIZING HCV PEPTIDE EPITOPES - A method and device to detect Hepatitis C (HCV) antibodies in oral fluid is provided. This method introduces a non-antibody detection molecule that labels all classes of patient antibodies in oral fluid, followed by the specific concentration of labeled anti-HCV antibodies by selective capture in a trapping zone consisting of peptide antigens derived from the HCV genome. Signal generated by the labeled antibodies present in the trapping zone is proportional to the number of anti-HCV antibodies bound to the antigens present in the trapping zone. Presence of signal derived from the capture of antibody/detection molecule complexes in the trapping zone is indicative of past exposure to HCV. | 11-01-2012 |
20120276522 | Methods and Compositions for Determining Virus Susceptibility to Integrase Inhibitors - Methods and compositions for the efficient and accurate determination of HIV susceptibility to an integrase inhibitor and/or HIV replication capacity are provided. In certain aspects, the methods involve detecting in a biological sample a nucleic acid encoding an HIV integrase that comprises a primary mutation at codon 143, wherein the mutation at codon 143 does not encode arginine (R) or cysteine (C), and wherein the presence of the integrase-encoding nucleic acid in the biological sample indicates that the HIV has a decreased susceptibility to an integrase inhibitor or altered replication capacity relative to a reference HIV. In certain embodiments, the HIV also contains one or more secondary mutations in integrase. Also provided are methods for determining the selective advantage of a mutation or mutation profile based on the difficulty to create the mutation, and its effect on susceptibility to an integrase inhibitor or replication capacity. | 11-01-2012 |
20120276523 | LIQUID DROP DIAGNOSTIC ASSAYS - The present invention provides simple and inexpensive assays for the detection of virtually any analyte in any sample that is in liquid form or that can be solubilized. The assays utilize the fluid dynamics of drop evaporation whereby soluble materials, including analytes and particles binding thereto, are drawn to the edge of the drop and ultimately form a concentrated residual ring. The presence or absence of certain reagents can then be detected through a number of different approaches. | 11-01-2012 |
20120276524 | GENOTYPING METHOD - The present invention relates to a genotyping method, and more particularly to an ID sequence, which is assigned to each genotype, and a multiplex genotyping method which uses the ID sequence. When pyrosequencing is performed using the ID sequence, a unique and simple pyrogram can be obtained for each genotype. Thus, the use of the ID sequence makes it possible to genotype viral genes, disease genes, bacterial genes and identification genes in a simple and efficient manner. In addition, a genotyping primer of the invention can be used in various genotyping methods which are performed using dispensation orders and sequencing methods. | 11-01-2012 |
20120276525 | METHOD AND SYSTEM FOR PREVENTING VIRUS-RELATED OBESITY AND OBESITY RELATED DISEASES - A method for preventing obesity related to infection by an adipogenic adenovirus includes assaying a sample from a person to determine whether the person has been previously infected with an adipogenic adenovirus, and if the person has not been previously infected, providing the person with at least one sensor positioned to detect when a person's hand approaches a predetermined distance from the person's face. By warning the person of undesired hand-to-face contacts, the person is able to reduce the incidence of obesity related infections. Other embodiments are directed to a kit for preventing obesity caused by infection with an adipogenic adenovirus, such kit including a container for assaying an agent indicating the presence of antibodies to Ad-36, and a sensor positioned on an item selected from the group consisting of one of a hat, a writing instrument, eye glasses, a belt, sunglasses, a bra, a shirt, and a tie. | 11-01-2012 |
20120276526 | METHOD AND SYSTEM FOR MONITORING AND RECORDING VIRAL INFECTION PROCESS AND SCREENING FOR AGENTS THAT INHIBIT VIRUS INFECTION - The present invention relates to a method for monitoring and recording a viral infection process, which is characterized by providing a microcantilever detection device, which comprises a microcantilever comprising a contact area having an macromolecular material attached thereon; loading host cells to the contact area to allow the host cells to be attached to the macromolecular material; loading virus to the contact area to make the virus to contact the host cells attached thereto whereby a deflection level of the microcantilever is produced; and recording the deflection level in a time course manner so as to obtain a deflection curve that can be used as a basis for monitoring and recording the viral infection process. The method of the invention can also be used for screen for an agent that inhibits virus infection. | 11-01-2012 |
20120282593 | Method and Kit for Detecting Virulent Strains of Influenza Virus - The present invention is a kit and method for determining the virulence of an influenza virus based upon the presence or absence of leucine at position 62, arginine at position 75, arginine at position 79 and leucine at position 82 of the polymerase basic 1-F2 protein amino acid sequence. | 11-08-2012 |
20120282594 | METHOD OF ENHANCED DETECTION FOR NANOMATERIAL-BASED MOLECULAR SENSORS - Sensors based on single-walled carbon nanotubes and graphene which demonstrate extreme sensitivity as reflected in their electrical conductivity to gaseous molecules, such as NO, NO | 11-08-2012 |
20120282595 | HIGH THROUGHPUT CELL-BASED HPV IMMUNOASSAYS FOR DIAGNOSIS AND SCREENING OF HPV-ASSOCIATED CANCERS - Methods for quantifying an HPV protein expression in a clinical sample are disclosed. The quantifying methods include the process for obtaining the clinical sample. Such a clinical sample is consisted of a population of cells that are susceptible to infection by an HPV. The quantifying methods also include the process for depositing the clinical sample into a container. The clinical sample is contacted with the first antibody that specifically binds to an HPV protein which is expressed by an HPV-infected cell under a condition that promotes specific binding of the first antibody to the HPV protein expressed by the population of cells. The methods further include the process for quantifying the specific binding of the first antibody and thereby quantifying the HPV protein expression in the clinical sample. The assay provides an objective test to identify patients with high-grade precursor from cytology samples before biopsy. | 11-08-2012 |
20120282596 | Sensor for Biomolecules - A method for sensing biomolecules in an electrolyte includes exposing a gate dielectric surface of a sensor comprising a silicon fin to the electrolyte, wherein the gate dielectric surface comprises a dielectric material and antibodies configured to bind with the biomolecules; applying a gate voltage to an electrode immersed in the electrolyte; and measuring a change in a drain current flowing in the silicon fin; and determining an amount of the biomolecules that are present in the electrolyte based on the change in the drain current. | 11-08-2012 |
20120288849 | METHOD AND SYSTEM FOR ROBUST AND SENSITIVE ANALYSIS OF BEAD-BASED ASSAYS - Computer-implemented methods and systems are provided for the analysis of multiplex fluorescent-dyed microsphere assays. The methods of the invention provide for determination of differences in analyte quantities between samples obtained from multiplex fluorescent-dyed microsphere assays by analysis of individual bead fluorescence and adjusting for variance; variance-stabilization of the data, and determining significance with hypothesis testing with tolerance determined by power estimation. The methods of the invention provide a benefit in allowing access to low signal or poor quality data, increased statistical power and decreased variability compared to standard curve methodology. | 11-15-2012 |
20120288850 | METHODS FOR DIAGNOSIS OF IMMUNE RESPONSES AGAINST VIRUSES - The present invention relates to methods for diagnosis of a cellular immune responses against a virus using an inactivated virus. In the method of the invention a cellular immune response against the virus is detected in a subject by incubating PBMCs from the subject with a preparation of inactivated virus and subsequently detecting the expression of at least one T cell specific cytokine in the subject's PBMCs, preferably by flow cytometry. Advantageously in the method, inactivated virus is used for incubation with PBMCs from the subject so as to make feasible that the method is performed in laboratories without BSL-3 classification. Preferably the method is a method for detecting a CD4+ and/or CD8+ T cell response against an influenza virus by detecting expression of one or more of CD107, IFN-γ, IL-2, IL-10 and TNF-α, using formalin inactivated influenza virus. The invention further pertains to kits comprising components that are useful for detecting a cellular immune responses in a subject against a virus. | 11-15-2012 |
20120288851 | Measurement System, Measurement Method, Program for Implementing the Method, and Recording Medium for the Program - The invention relates to a measurement system MS | 11-15-2012 |
20120288852 | Force Mediated Assays - A sensitive and specific method of detecting chemical species, viruses and microorganisms is presented to improve performance of molecular-recognition-based assays utilizing particles decorated with molecular recognition agents such as antibodies and DNA probes, and observing analyte-dependent changes in the response of the particles to forces such as magnetic or gravitational forces or Brownian thermal fluctuations. | 11-15-2012 |
20120295249 | Instrument And Method For The Automated Thermal Treatment Of Liquid Samples - An instrument and a method for the automated thermal treatment of liquid samples are disclosed. An inter-distance between a temperature-controlled receptacle for loading with a plurality of vessels for containing the samples and end portions of optical fibers can be varied, wherein the receptacle is configured to form a thermal communication with the loaded vessels and wherein the optical fibers have first and second end portions. The first end portion and the second end portion of each optical fiber is fixed with respect to each other for transmitting light, wherein the variation of the inter-distance allows the vessels to be loaded to or unloaded from the receptacle and to enable detection of light from the samples contained in the one or more receptacle-loaded vessels. | 11-22-2012 |
20120295250 | Microchip, Measurement System and Method Using the Same, and Test Reagent to be Used for Microchip - A microchip to be used for measuring a plurality of types of objects to be measured. The microchip includes at least a reagent retaining portion and a detecting portion. The test reagent retaining portion includes a plurality of types of test reagents corresponding respectively to the plurality of types of objects to be measured. A plurality of time courses for a change in detected value at the detecting portion caused by a reaction between the test reagents and the objects to be measured corresponding respectively thereto are all different from each other. | 11-22-2012 |
20120295251 | DETERMINING CODON DISTRIBUTION AND/OR BASE PAIR DISTANCE BETWEEN CODONS IN A NUCLEIC ACID - The present invention relates to methods for the design and/or production of a probe or primer that is capable of hybridizing to a plurality of sites in a sample comprising nucleic acid. Furthermore, the present invention provides methods for detecting and amplifying nucleic acid using such a probe or primer, for example, for identification of a strain, species or genera. Probe or primer sequences are determined by reference to codon usage bias of a target nucleic acid. In addition, the present invention provides methods for determining codon distribution and/or base pair distance between codons in a nucleic acid. | 11-22-2012 |
20120301871 | COMPARATIVE LIGAND MAPPING FROM MHC POSITIVE CELLS - The present invention relates generally to a methodology for the isolation, purification and identification of peptide ligands presented by MHC positive cells. In particular, the methodology of the present invention relates to the isolation, purification and identification of these peptide ligands from soluble class I and class II MHC molecules which may be uninfected, infected, or tumorgenic. The methodology of the present invention broadly allows for these peptide ligands and their comcomittant source proteins thereof to be identified and used as markers for infected versus uninfected cells and/or tumorgenic versus nontumorgenic cells with said identification being useful for marking or targeting a cell for therapeutic treatment or priming the immune response against infected cells. | 11-29-2012 |
20120301872 | SYSTEM AND METHOD FOR INCREASED FLUORESCENCE DETECTION - The invention relates to systems and methods for improving optical detection and sensitivity in situations in which emission of luminescent light is monitored. It is provided a sample carrier which achieves increased sensitivity of luminescent light detection. The sample carrier comprises a sample carrying part and a light reflecting part; wherein the light reflecting part is positioned to allow an optical collection and detection system to collect not only luminescent light emitted from the sample positioned on the sample carrying part in a direction of the optical collection and detection system, but also luminescent light emitted from the sample in a direction away from the optical collection and detection system and reflected in the direction of the optical collection and detection system via the light reflecting part. When an excitation light source is needed, the light reflecting part also allows the excitation light rays passing through the sample to hit the light reflecting part, and reflect back in the opposite direction thus the reflected excitation light also passes through the sample. Also provided are methods of using such a sample carrier. | 11-29-2012 |
20120308992 | DRY STICK DEVICE AND METHOD FOR DETERMINING AN ANALYTE IN A SAMPLE - A method of preparing a dry stick test device for determining an analyte in a milk sample by chemical assay. At least one reagent pad is provided by impregnating a first porous material with an aqueous solution including a reagent capable of reacting with the analyte, a derivative of the analyte or an indicator compound for the analyte to provide a detectable signal when in a moistened state. The reagent pad is dried. A development pad is provided by impregnating a second porous material with an aqueous solution including at least one controlling compound which, when in a moistened state, is capable of providing a condition required for the reagent to react with the analyte to provide the detectable signal. The impregnated second porous material is dried. The first porous material is immobilized with the second porous material, on a solid support, to obtain the dry stick test device. | 12-06-2012 |
20120308993 | RESISTANT INFLUENZA A VIRUS DETECTION METHOD AND KIT THEREFOR - There is provided a method of detecting the presence of oseltamivir-resistant Influenza A virus in a sample, the method comprising detecting the presence of an amplicon, a fragment and/or a derivative thereof, wherein the amplicon, fragment and/or derivative thereof (a) is amplified by a primer (SEQ ID NO:1), a fragment, or derivative therefore and/or a primer (SEQ ID NO:2), a fragment, or derivative thereof. There are also provided primer(s) and/or probe as well as kit for the detection of the presence of Influenza A in a sample and/or in a subject. | 12-06-2012 |
20120308994 | SLPA AS A TOOL FOR RECOMBINANT PROTEIN AND ENZYME TECHNOLOGY - Disclosed are a recombinant DNA molecule encoding a fusion protein comprising a SlpA chaperone and a target polypeptide wherein human FK506 binding proteins (FKBPs) are excluded as target polypeptides, a corresponding expression vector encoding said fusion protein as well as host cells transformed with said expression vector. Also disclosed are a method for producing the fusion protein, a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. A further aspect of the invention is the use of the recombinantly produced fusion protein, and a reagent kit containing a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. | 12-06-2012 |
20120308995 | VACCINIA VIRUS MUTANTS CONTAINING THE MAJOR GENOMIC DELETIONS OF MVA - The present invention provides modified vaccinia virus (VACV) genomes as well as vectors, especially viral vectors comprising the same. The present invention further provides modified vaccinia viruses. The present invention further provides methods for determining the effect of mutations in VACV with regard to competence for replication in certain cell types. The present invention further provides methods of preparing modified vaccinia viruses. | 12-06-2012 |
20120308996 | METHOD AND SYSTEM FOR DISEASE DIAGNOSIS VIA SIMULTANEOUS DETECTION OF ANTIBODIES BOUND TO SYNTHETIC AND CELLULAR SUBSTRATES - The invention relates to a method and system for disease diagnosis that simultaneously detects antibodies bound to cellular and/or tissue substrates and antibodies bound to synthetic substrates, such as microparticles or beads coated with specific antigens, thereby providing a “one-step” method for the simultaneous detection and characterization of disease-associated antibodies at both low (cellular and/or tissue) and high (antigen) specificity. | 12-06-2012 |
20120315621 | PERSONAL GLUCOSE METERS FOR DETECTION AND QUANTIFICATION OF A BROAD RANGE OF ANALYTES - A methodology for developing highly sensitive and selective sensors that can achieve portable, low-cost and quantitative detection of a broad range of targets using only a personal glucose meter (PGM) is disclosed. The method uses recognition molecules specific for a target agent, enzymes that can convert an enzyme substrate into glucose, and a PGM. Also provided are sensors, which can include a solid support having attached thereto a recognition molecule that permits detection of a target agent, as well as an enzyme that can catalyze the conversion of a substance into glucose, wherein the enzyme is attached directly or indirectly to the recognition molecule, and wherein in the presence of the target agent the enzyme can convert the substance into glucose. The disclosed sensors can be part of a lateral flow device. Methods of using such sensors for detecting target agents are provided. | 12-13-2012 |
20120315622 | SYSTEM FOR DETECTING AND ENUMERATING BIOLOGICAL PARTICLES - The invention discloses a system to detect and enumerate diverse biological particles through the use of microbial spores that in the presence of a redox substrate rapidly respond to germination signals by forming discrete intracellular fluorescent formazan granules. The disclosed system enables ultrasensitive detection and enumeration of different analytes including microorganisms, viruses, nucleic acids, polypeptides, and natural or man-made particles bearing analytes. | 12-13-2012 |
20120315623 | DETECTION OF CONDUCTIVE POLYMER-LABELED ANALYTES - The disclosure relates to the detection of analytes (e.g., biological pathogens such as bacteria or viruses) using a conductive polymer label. The disclosed detection system utilizing the conductive polymer label generally involves the formation of an analyte conjugate between the target analyte and a conductive polymer moiety conjugated to the target analyte. The conductive polymer portion of the analyte conjugate is electrically activated to form an electrically activated analyte conjugate having an increased electrical conductivity relative to the analyte conjugate as originally formed. The electrically activated analyte conjugate can then be detected by any suitable means, such as by conductimetric or electrochemical detection. | 12-13-2012 |
20120315624 | Using LNA Flow-Fish to Quantitatively Monitor Viral Infections in Infected Cells and Test the Efficacy of Antiviral Medications - As described herein, locked nucleic acids are used with flow cytometric-fluorescence in situ hybridization (LNA flow-FISH) detection of viral RNA in infected cells. This technique represents a straightforward way to monitor viral infection in cells and can be used to measure efficacy of potential antiviral compounds. | 12-13-2012 |
20120315625 | SELF-ASSEMBLED MONOLAYERS AND METHODS FOR USING THE SAME IN BIOSENSING APPLICATIONS - Cross-linked amphiphile constructs that form self-assembled monolayers (SAMs) on metal surfaces such as gold surfaces are disclosed. These new SAMs generate well packed and highly oriented monolayer films on gold surfaces. A method for using the SAMs in the fabrication of biomolecule sensors is also disclosed. | 12-13-2012 |
20120315626 | METHOD OF EVALUATING ELIMINATION OF MICROOGANISMS AND APPARATUS FOR EVALUATING ELIMINATION OF MICROORGANISMS - The sterilizing effect of particle irradiation on microorganisms for the sterilizing treatment thereof can be evaluated. The evaluation can be done by supplying microorganisms in the space inside a container ( | 12-13-2012 |
20120315627 | METHOD FOR DETERMINING THE EFFECTIVENESS OF STERILIZATION AND/OR DISINFECTION PROCESS - A method for determining the effectiveness of a sterilization and/or disinfection process is disclosed. | 12-13-2012 |
20120322048 | Materials and Methods for Assessing and Mapping Microbes and Microbial Biofilms on Wounds - The subject invention provides point-of-care assays for assessing the topographical distribution of microbial biofilm and/or specific microorganisms in wounds. | 12-20-2012 |
20120322049 | MATERIALS AND METHODS FOR DETECTION OF HPV NUCLEIC ACIDS - Provided are nucleic acids capable of hybridizing to HPV 16 and/or HPV 18 nucleic acids, in particular, mRNA encoding E2 and E6-7 gene products. Such nucleic acids are useful in methods of isolating RNA from a biological sample, methods and means for determining the presence of particular RNA splice-form variants in a biological sample, methods and means for determining the relative ratio of RNA ratios in a biological sample, methods and means for predicting the progression of precancerous cervical lesions, and methods and means for detecting disruption of genes or gene expression. | 12-20-2012 |
20120322050 | USING IMPEDANCE-BASED CELL RESPONSE PROFILING TO IDENTIFY PUTATIVE INHIBITORS FOR ONCOGENE ADDICTED TARGETS OR PATHWAYS - Use of impedance devices in methods of generating a time dependent cellular profiles (TCRP) for the modulation of oncogene addicted cells and comparing the impedance-based TCRP to controls or knowns to identify signature time dependent cellular profiles. | 12-20-2012 |
20120322051 | METHODS AND COMPOSITIONS FOR IDENTIFYING AND CHARACTERIZING HEPATITIS C - The invention provides novel methods and compositions for amplifying portions of the HCV genome. The nucleic acid sequences set forth as SEQ ID NOS:1-64 derived from HCV cDNA and functional equivalents thereof, kits containing same, and methods employing same, are useful for the identification and characterization of HCV in biological samples. | 12-20-2012 |
20120322052 | SYSTEM AND METHOD FOR PREPARING SAMPLES - A system and method for preparing samples for analyte testing. The sample preparation system can include a freestanding receptacle. The method can include providing a liquid composition comprising a source and a diluent, and positioning the liquid composition in a reservoir defined by the freestanding receptacle. The method can further include filtering the liquid composition to form a filtrate comprising an analyte of interest, removing at least a portion of the filtrate from the sample preparation system to form a sample, and analyzing the sample for the analyte of interest. | 12-20-2012 |
20120322053 | CROSS-COUPLED PEPTIDE NUCLEIC ACIDS FOR DETECTION OF NUCLEIC ACIDS OF PATHOGENS - The present invention concerns methods for detecting a nucleic acid comprising (i) contacting a solution comprising a first PNA having a first cross-reactive functional group with a substrate having a second PNA affixed thereto, the second PNA having a second first cross-reactive functional group, wherein the first PNA has a reporter molecule attached thereto and the first and second PNAs being complementary to different portions of a target DNA; (ii) contacting a sample suspected of containing the nucleic acid with the first and second PNAs; and (iii) determining the presence of the reporter molecule on the substrate. | 12-20-2012 |
20120329037 | BIOSENSOR - Embodiments of the present disclosure set forth a biosensor for detecting a target. One example sensor includes a first electrode. The first electrode includes a first electron conducting molecule and a first probe. The first probe includes a second electron conducting molecule. The first probe is configured to bind to the target of interest in solution. The first and second electron conducting molecules are different. | 12-27-2012 |
20120329038 | Multivolume Devices, Kits and Related Methods for quantification of Nucleic Acids and Other Analytes - Provided are devices comprising multivolume analysis regions, the devices being capable of supporting amplification, detection, and other processes. Also provided are related methods of detecting or estimating the presence nucleic acids, viral levels, and other biological markers of interest. | 12-27-2012 |
20120329039 | SUBSTANCE DETERMINING APPARATUS - The invention relates to a substance determining apparatus for determining a substance within a fluid. Particles attach the substance and bind to a binding surface ( | 12-27-2012 |
20120329040 | MICROFLUIDIC DEVICES AND METHODS FOR SEPARATING AND DETECTING CONSTITUENTS IN A FLUID SAMPLE - Microfluidic devices and methods for using the same are provided. Aspects of the present disclosure include microfluidic devices that include a separation medium having functional groups which covalently bond to one or more analytes of interest, e.g., proteins, in a sample upon application of an applied stimulus, e.g., light. Also provided are methods of using the devices as well as systems and kits that include the devices. The devices, systems and methods find use in a variety of different applications, including diagnostic and validation assays. | 12-27-2012 |
20120329041 | REAL-TIME PCR POINT MUTATION ASSAYS FOR DETECTING HIV-1 RESISTANCE TO ANTIVIRAL DRUGS - Disclosed are compositions including primers and probes, which are capable of interacting with the disclosed nucleic acids, such as the nucleic acids encoding the reverse transcriptase or protease of HIV as disclosed herein. Thus, provided is an oligonucleotide comprising any one of the nucleotide sequences set for in SEQ ID NOS:1-89, and 96-104. Also provided are the oligonucleotides consisting of the nucleotides as set forth in SEQ ID NOS:1-89, and 96-104. Each of the disclosed oligonucleotides is a probe or a primer. Also provided are mixtures of primers and probes and for use in RT-PCR and primary PCR reactions disclosed herein. Provided are methods for the specific detection of several mutations in HIV. Mutations in both the reverse transcriptase and the protease of HIV can be detected using the methods described herein. | 12-27-2012 |
20130004939 | Fetuin-beads, Manufacturing method thereof and method of concentrating and detecting influenza virus by the same - Fetuin-beads, a manufacturing method thereof and a method of concentrating and detecting influenza virus by the fetuin-beads are disclosed. The method of concentrating and detecting an influenza virus by the fetuin-beads comprises the steps of preparing fetuin-beads; mixing the fetuin-beads and a solution capable of providing salt ions to obtain a fetuin-bead solution; mixing the fetuin-bead solution and a sample comprising the influenza virus to concentrate the influenza virus onto the fetuin-beads to obtain a plurality of fetuin bead-influenza virus combinations; and collecting the fetuin bead-influenza virus combinations and using a virus testing method to detect the influenza virus on the fetuin bead-influenza virus combinations. Therefore, an easy and fast method to detect the influenza virus with timeliness and high accuracy is provided. | 01-03-2013 |
20130004940 | Device for Detecting Analyte in Sample - A device for detecting an analyte in a sample is provided, which comprises: a collecting chamber containing an opening for collecting a liquid sample; a detecting element for detecting an analyte in the liquid sample; and a lid for covering the opening of the collecting chamber. The device further comprises an indicating device thereon to indicate whether or not the lid covers at an appointed position. The operation of the device is very simple. | 01-03-2013 |
20130004941 | PROTEINASE K INHIBITORS, METHODS AND COMPOSITIONS THEREFOR - The synthesis, biological evaluation, and molecular modeling of alkoxysuccinyl-peptidyl-haloalkyl ketones for use as proteinase K inhibitors are described. Sample preparation processes for in situ RNA or DNA analysis using such inhibitors, methods and compositions therefor are provided. | 01-03-2013 |
20130004942 | METHODS OF TESTING FOR INTRACELLULAR PATHOGENS - A first intracellular pathogen in a biological sample that may contain more than one intracellular pathogen is studied by a method comprising the steps of (i) contacting the sample with a population of cells in the presence of an agent inhibiting the reproduction of a second intracellular pathogen; (ii) incubating the cells under conditions that permit the reproduction of the first intracellular pathogen; and (iii) testing material arising from step (ii) for the first intracellular pathogen. | 01-03-2013 |
20130004943 | H5 Subtype-Specific Binding Proteins Useful for H5 Avian Influenza Diagnosis and Surveillance - The invention provides monoclonal antibodies and related binding proteins that bind specifically to the envelope glycoprotein of H5 subtypes of avian influenza virus (“AIV”). The monoclonal antibodies and related binding proteins are useful for the detection of H5 subtypes of AIV, including the pathogenic H5N1 subtypes. Virus may be detected in formalin preserved, paraffin embedded specimens as well as frozen specimens and biological fluids. Accordingly, the invention provides means for the diagnosis and surveillance of dangerous viral infections. | 01-03-2013 |
20130004944 | H5 Subtype-Specific Binding Proteins Useful for H5 Avian Influenza Diagnosis and Surveillance - The invention provides monoclonal antibodies and related binding proteins that bind specifically to the envelope glycoprotein of H5 subtypes of avian influenza virus (“AIV”). The monoclonal antibodies and related binding proteins are useful for the detection of H5 subtypes of AIV, including the pathogenic H5N1 subtypes. Virus may be detected in formalin preserved, paraffin embeded specimens as well as frozen specimens and biological fluids. Accordingly, the invention provides means for the diagnosis and surveillance of dangerous viral infections. | 01-03-2013 |
20130011826 | METHODS AND KITS FOR DECREASING INTERFERENCES FROM LEUKOCYTES IN SPECIFIC BINDING ASSAYS - The present disclosure describes methods and kits for reducing interferences in immunoassays performed on solid phase and on samples containing serum or plasma, by adding an effective amount of a polycationic derivative of dextran to the assay. | 01-10-2013 |
20130011827 | METHODS AND KITS FOR DECREASING INTERFERENCES IN PLASMA OR SERUM CONTAINING ASSAY SAMPLES OF SPECIFIC BINDING ASSAYS - Methods and kits are provided for decreasing interferences and inaccuracies due to nonoptimal sample handling of blood samples in plasma or serum containing assay samples of specific binding assays by addition of a large polycation to the assay sample during the specific binding assay. | 01-10-2013 |
20130011828 | Use - The present invention relates to the use of one or more cas genes for modulating resistance in a cell against a target nucleic acid or a transcription product thereof. | 01-10-2013 |
20130011829 | DEVICE AND METHODS FOR QUANTIFYING ANALYTES - The present invention relates to devices and methods for measuring the quantity of multiple analytes in a sample. The device is designed such that each of the analyte sensing elements is configured to measure the quantity of a predetermined analyte and where the machine executable instructions are configured to select the proper analyte sensing element corresponding to the analyte to be measured. | 01-10-2013 |
20130011830 | FAST RESULTS HYBRID CAPTURE ASSAY AND SYSTEM - The present invention comprises a method that provides fast and reliable results for detecting the presence of a target nucleic acid molecule in a sample. | 01-10-2013 |
20130017534 | DEVICE AND METHOD FOR IDENTIFYING MICROBES AND COUNTING MICROBES AND DETERMINING ANTIMICROBIAL SENSITIVITY - A method of determining antimicrobial activity of an agent can include providing a well, wherein the well contains at least one antimicrobial agent, the well further including at least two electrodes. A sample of a microbe can be added into the well and a voltage pulsed between the electrodes. An electrical property can be sampled and recorded. In another aspect, a method of identifying at least one microbe includes taking a sample containing the at least one microbe, isolating the at least one microbe from the sample, dividing the at least one microbe into a at least one well, wherein each well contains at least one antimicrobial agent and at least two electrodes. A voltage is pulsed between the at least two electrodes, an electrical property is sampled during the pulsing and recorded. In another aspect, a diagnostic device for detecting at least one microbe is presented. | 01-17-2013 |
20130017535 | INSTRUMENT AND PROCESS FOR THE AUTOMATED PROCESSING OF LIQUID SAMPLESAANM Frey; RolfAACI EbikonAACO CHAAGP Frey; Rolf Ebikon CHAANM Schacher; GottliebAACI KriensAACO CHAAGP Schacher; Gottlieb Kriens CH - An automated instrument and process for processing samples is presented. The instrument comprises a sampling area for receiving samples and reaction vessels; an analytical area with a first device resource comprising at least one analyzer; a reaction area comprising a conveyor for reagent containers; an incubator; a second device resource comprising first functional devices, the first functional devices having access to the sampling area and the incubator such as to transfer reaction vessels from the sampling area to the incubator and/or to pipette samples and/or reagents into the reaction vessels; a third device resource comprising second functional devices, the second functional devices having access to the incubator and the analytical area such as to transfer the reaction vessels from the incubator to the analytical area and/or to dispense liquids or withdraw liquids from the reaction vessels. | 01-17-2013 |
20130017536 | WESTERN BLOT KIT FOR DETECTION OF VACCINATED POULTRYAANM Madani; RasoolAACI KarajAACO IRAAGP Madani; Rasool Karaj IRAANM Rezayat; Seyed MahdiAACI TehranAACO IRAAGP Rezayat; Seyed Mahdi Tehran IRAANM Sarkar; SaeedAACI TehranAACO IRAAGP Sarkar; Saeed Tehran IRAANM Emami; TaraAACI KarajAACO IRAAGP Emami; Tara Karaj IR - Modified western blot membranes with silver nanoparticle allow the small peptides of the NS1 protein of the poultry influenza virus to be kept in the membrane and not to diffuse during transferring from the Tricine SDS PAGE. These peptides may differentiate infected from vaccinated poultry. | 01-17-2013 |
20130022960 | RAPID TEST FOR DETECTING INFECTION - Test kit has a cellulose filter paper with a flow rate of about 0.04 to about 0.4 ml/min/cm | 01-24-2013 |
20130022961 | ASSAY FOR JC VIRUS ANTIBODIES - The disclosure relates to methods and reagents for analyzing samples for the presence of JC virus antibodies. Disclosed is a method that includes obtaining a biological sample from a subject (e.g., plasma, serum, blood, urine, or cerebrospinal fluid), contacting the sample with highly purified viral-like particles (HPVLPs) under conditions suitable for binding of a JCV antibody in the sample to an HPVLP, and detecting the level of JCV antibody binding in the sample to HPVLP. In one embodiment, determining the level of anti-JCV antibodies in the subject sample provides a method of identifying PML risk in a subject. | 01-24-2013 |
20130022962 | METHOD AND APPARATUS FOR DIAGNOSTIC ANALYSES - A method for diagnostic analyses, in particular to identify pathogens, such as viruses, bacteria or other micro-organisms present in a biological sample, comprises a first step of measuring and continuously monitoring the turbidity and/or the concentration of the pathogens, by means of an instrumental reading technique, of a liquid culture medium into which the sample to be analyzed has been inoculated and in which the replication of the pathogens possibly present occurs, said measuring and monitoring being carried out dynamically during the replication of the pathogens growing in the culture medium; and a second step of identifying the pathogens, carried out by taking at least an aliquot of the liquid culture medium containing the biological sample directly obtained from the first step, which has reached a desired value of turbidity according to a standardized value scale, such as the McFarland turbidity scale, and/or of concentration of the pathogens, and using said aliquot directly in mass spectrophotometric identification means ( | 01-24-2013 |
20130022963 | DIRECT AMPLIFICATION AND DETECTION OF VIRAL AND BACTERIAL PATHOGENS - Provided herein are methods for identifying the presence or absence of a target nucleic acid from a microorganism using direct amplification without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids. | 01-24-2013 |
20130022964 | Use of Ribozymes in the Detection of Adventitious Agents - The present invention provides a method of detecting adventitious agents in a composition comprising a microorganism by using ribozyme-expressing indicator cells, as well as indicator cells useful in such detection. | 01-24-2013 |
20130029316 | METHOD FOR REAL-TIME DETECTION OF WEST NILE VIRUS USING A CLEAVABLE CHIMERIC PROBE - A method is described for the real-time detection of West Nile Virus in samples taken from humans or potential carriers of the virus such as mosquitoes or birds. Real-time detection of West Nile Virus is performed in a PCR reaction using gene specific primers and a cleavable chimeric fluorescent probe. The method is amenable to medium and high throughput analysis. | 01-31-2013 |
20130029317 | METHOD FOR EARLY DIAGNOSIS OF LIVER CANCER AND PREDICTION OF METASTASIS - Disclosed is a method for early diagnosis of liver cancer. The method comprises the steps of: (A) providing a sample obtained from a subject; (B) assessing the expression level of four subtypes of α-mannosidase genes consisting of MAN1C1 in the sample; (C) comparing the expression level of α-mannosidase genes in the sample with a normal control; and (D) determining whether the subject having a risk of suffering liver cancer in accordance with the result of step (C); wherein while the MAN1C1 expression level of the sample is lower than that in the normal control, the subject is determined to have a risk of suffering liver cancer. Additionally, while MAN1A1, MAN1A2 and MAN1B1 expression levels in the sample are higher than those in control group, the subject is determined to suffer from liver cancer and has a risk of metastasis. | 01-31-2013 |
20130029318 | Microchips and Methods for Testing a Fluid Sample - Systems and methods for medical diagnosis or risk assessment for a patient are provided. A fluid-testing microchip is described which includes a filter compartment configured to receive a fluid sample from an inlet port, wherein the filter compartment comprises a plurality of beads coated with a defoaming agent, a micro-pump configured to transfer the fluid sample from the filter compartment to a test compartment, and the test compartment comprising a test component configured to test the fluid sample. The systems include an instrument for reading or evaluating the test data. These systems and methods are designed to be employed at the point of care, such as in emergency rooms and operating rooms, or in any situation in which a rapid and accurate result is desired. | 01-31-2013 |
20130029319 | MEANS AND METHODS FOR PREDICTING THE RISK OF MORTALITY OF PATIENTS WITH HPV POSITIVE OROPHARYNGEAL SQUAMOUS CELL CANCER - The present invention relates to the field of diagnostic measures. In particular, the present invention relates to a method for predicting the risk of mortality in a subject suffering from low viral load HPV (human papillomavirus) positive oropharyngeal squamous cell cancer. The method is based on the determination of the amount of an E6* gene product and the amount an E1̂E4 gene product of HPV in a sample from said subject. Moreover, the method is based on determining the presence of absence of an E1C gene product of HPV. The present invention further relates to a method for predicting the risk of mortality in a subject suffering from HPV (human papillomavirus) positive oropharyngeal squamous cell cancer based on the determination of copy number of HPV per cancer cell. | 01-31-2013 |
20130029320 | DEVICE AND METHOD FOR DETECTING BIOMOLECULE - Disclosed are a method for detecting a biomolecule including: immobilizing a nucleic acid aptamer capable of specifically binding to a biomolecule to be detected on the surface of a bead on which fluorophores are arranged; hybridizing the nucleic acid aptamer with a guard nucleic acid (g-nucleic acid) labeled with a quencher to quench fluorescence; and reacting a sample including the biomolecule to be detected with the nucleic acid aptamer and detecting a fluorescence signal emitted as the biomolecule binds with the nucleic acid aptamer and the g-nucleic acid labeled with the quencher is separated, and a device for detecting a biomolecule for conducting the detection method. The present disclosure allows for effective, convenient and fast detection of the biomolecule to be detected, enables quantitative analysis, and enables detection of even a trace amount of sample. | 01-31-2013 |
20130029321 | SAMPLE PROCESSING DEVICE AND METHOD - A sample processing device is disclosed, which sample processing device comprises a first substrate and a second substrate, where the first substrate has a first surface comprising two area types, a first area type with a first contact angle with water and a second area type with a second contact angle with water, the first contact angle being smaller than the second contact angle. The first substrate defines an inlet system and a preparation system in areas of the first type which two areas are separated by a barrier system in an area of the second type. The inlet system is adapted to receive a sample liquid comprising the sample and the first preparation system is adapted to receive a receiving liquid. In a particular embodiment, a magnetic sample transport component, such as a permanent magnet or an electromagnet, is arranged to move magnetic beads in between the first and second substrates. | 01-31-2013 |
20130029322 | ANTI-HPV E7 ANTIBODIES - Monoclonal anti-HPV (human papillomavirus) E7 antibodies are capable of specifically recognizing an epitope of the C-terminal or the N-terminal region of a HPV E7 protein. | 01-31-2013 |
20130034843 | Molecular Determinants Associated with Enhanced Ability to Enter Cells Expressing CXCR4 - The invention provides a method for determining whether a human immunodeficiency virus is likely to be have enhanced ability to enter a cell expressing CD4 and CXCR4 relative to a reference HIV. In certain aspects, the methods comprise detecting one or more amino acids in an envelope protein of the HIV associated with enhanced ability to enter CD4- and CXCR4-expressing cells and determining that the HIV's ability to enter such cells is enhanced relative to a reference HIV, e.g., an HIV that does not comprise such amino acid(s). | 02-07-2013 |
20130034844 | ASSAY FOR MEASURING CELL-MEDIATED IMMUNORESPONSIVENESS - The present invention relates generally to the field of immunological-based diagnostic assays. More particularly, the present invention contemplates a method for measuring cell-mediated immunoresponsiveness. The present invention further enables determination of the immunosuppressive effects of disease conditions, therapeutic agents and environmental contaminants. The assay of the present invention is also capable of integration into pathology architecture to provide a diagnostic reporting system and to facilitate point of care clinical management. | 02-07-2013 |
20130034845 | BARRIERS FOR FACILITATING BIOLOGICAL REACTIONS - The present invention relates to systems, devices, and methods for performing biological reactions. In particular, the present invention relates to the use of lipophilic, water immiscible, or hydrophobic barriers in sample separation, purification, modification, and analysis processes. | 02-07-2013 |
20130040283 | GRAPHENE COMPOSITION, METHOD OF FORMING A GRAPHENE COMPOSITION AND SENSOR SYSTEM COMPRISING A GRAPHENE COMPOSITION - A method of forming a composition includes oxidation of graphene oxide to form holey graphene oxide having defects therein and reduction of the holey graphene oxide. | 02-14-2013 |
20130040284 | HBV DRUG RESISTANCE METHODS - New polymorphisms in the nucleic acid sequences of the DNA polymerase/reverse transcriptase open reading frame and viral surface antigen open reading frame of the hepatitis B virus are reported. In particular, the present invention relates to the mutation YMDD→YSDD in the HBV reverse transcriptase domain and to the W196V mutation in the small HBV viral surface antigen. Said polymorphisms are affecting the detection of drug resistance mutations by genotypic methods and diagnostic kits based thereon. The present invention relates to methods and diagnostic kits for detection of a HBV virus comprising said nucleic acid polymorphisms. In particular, those methods utilizing oligonucleotides capable of hybridizing to said HBV nucleic acid polymorphisms are envisaged. | 02-14-2013 |
20130040285 | Saliva Polymerase Chain Reaction Assay for Cytomegalovirus Infection - Congenital cytomegalovirus (CMV) infection is an important cause of disease in infants and immune-compromised adults. Most infants infected with CMV are not promptly identified because of the absence of symptoms. It has been discovered that an assay based on the polymerase chain reaction (PCR) for testing of saliva specimens for CMV is rapid, accurate, and inexpensive. Some versions of the assay employ primers that are specific for sequences flanking the highly conserved major envelope glycoprotein B or the highly conserved immediate early 2 exon 5 genes. The assay exceeds the standard rapid culture technique in accuracy, speed, and economy. When the assay is performed on dried saliva, it loses no significant amount of accuracy, and is surprisingly much more sensitive than a PCR assay using dried blood. This assay will permit broader testing to detect and intervene in congenital CMV infection, potentially avoiding numerous cases of associated disease. | 02-14-2013 |
20130040286 | Rapid Diagnostic Device, Assay and Multifunctional Buffer - A rapid diagnostic device, assay and multifunctional buffer reagent are provided for the detection of a target analyte in a fluid test sample. The 2-step assay utilizes a dual component flow-through device comprising a test unit and a dried indicator reagent delivery unit for receiving the fluid sample and multifunctional buffer, respectively. The test unit comprises a reaction zone containing immobilized capture reagent that can bind to the target analyte, an absorbent zone supporting the reaction zone, and optionally, a blood separation zone in lateral fluid communication with the reaction zone. The delivery unit comprises a label zone permeated with a dried indicator reagent which can be placed in transient fluid communication with the reaction zone of the test unit during the assay procedure. The rapid diagnostic assay system reduces the number of assay reagents, method steps and time required for performance compared to other conventional assays. | 02-14-2013 |
20130040287 | Methods for Detection and Typing of Nucleic Acids - Disclosed are methods and kits for identifying and characterizing polynucleotide sequences in a sample which may include a heterogeneous sample. Some of the methods and kits are directed to the identification and characterization of a virus in a sample, which may include HIV capable of cause AIDS or AIDS-like symptoms. The virus may be HIV-1, and may also include drug resistant mutations. The methods may include reacting a mixture that includes, in addition to nucleic acid isolated from the sample, at least one oligonucleotide capable of specifically hybridizing to HIV nucleic acid where the oligonucleotide includes at least one non-natural base. In addition, the methods may include detection of one or more mutations in HIV nucleic acid that are associated with drug resistance. | 02-14-2013 |
20130040288 | Biological Specimen Collection and Transport System and Method of Use - Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. The disclosed compositions safely facilitate rapid sample collection, and provide extended storage and transport of the samples at ambient or elevated temperature without contamination of the sample or degradation of the nucleic acids contained therein. This process particularly facilitates the collection of specimens from remote locations, and under conditions previously considered hostile for preserving the integrity of nucleic acids released from lysed biological samples without the need of refrigeration or freezing prior to molecular analysis. | 02-14-2013 |
20130040289 | BREAST MILK ETHANOL SCREENING SYSTEM AND METHOD - A test kit detects the presence of a target analyte in a fluid sample. In particular, the test kit includes reagents capable of detecting the target analyte of interest in breast milk. More particularly, the test kit is capable of detecting the presence of alcohol, caffeine, nicotine, drugs of abuse, therapeutic drugs, triglycerides, lactose, capsaicin, and gluten, for example, in breast milk. | 02-14-2013 |
20130040290 | METHODS AND SYSTEMS FOR ULTRA-TRACE ANALYSIS OF LIQUIDS - A monitoring assembly ( | 02-14-2013 |
20130045474 | DEVICES AND METHODS FOR DETECTING AND MONITORING HIV AND OTHER INFECTIONS AND DISEASES - Disclosed herein are bio-nanosensor devices and methods suitable for blood assays. The bio-nanosensors are based on thickness shear mode transducer capable of transmitting a shear wave into a biofluid adjacent to a bio-functionalized sensing interface of a piezoelectric crystal. The bio-functionalized sensing interface includes one or more antibodies and/or biomarker-specific ligands capable of sensing HIV. The disclosed bio-nanosensors are capable of defecting the presence of HIV virus at picogram sensitivities using no more than 10 μl of blood in less than 15 minutes. | 02-21-2013 |
20130052637 | OPTICAL FLUORESCENT IMAGING IN HISTOLOGY - Compounds and methods are disclosed that are useful for noninvasive imaging in the near-infrared (NIR) spectral range. The NIR is highly sensitive for tumor detection and tracking. The application discloses targeting a tumor-enriched cell surface receptor with a ligand-conjugated fluorescent probe, which specifically allows detection of the tumor relative to the negligible animal autofluorescence. | 02-28-2013 |
20130059289 | ADENO-ASSOCIATED VIRUS SEROTYPE I NUCLEIC ACID SEQUENCES, VECTORS AND HOST CELLS CONTAINING SAME - The nucleic acid sequences of adeno-associated virus (AAV) serotype 1 are provided, as are vectors and host cells containing these sequences and functional fragments thereof. Also provided are methods of delivering genes via AAV-1 derived vectors. | 03-07-2013 |
20130059290 | DETECTION OF NUCLEIC ACIDS IN CRUDE MATRICES - A method includes contacting a crude matrix with components of an isothermal nucleic acid amplification reaction for a target nucleic acid species, thereby providing a mixture; incubating the mixture under conditions sufficient for the isothermal nucleic acid amplification reaction to proceed, thereby providing a product; and determining whether an indicator of the target nucleic acid species is present in the product. | 03-07-2013 |
20130059291 | COMPOSITIONS AND METHODS FOR IDENTIFYING DENGUE VIRUS - The invention generally relates to compositions and methods for identifying dengue virus. In certain embodiments, the invention provides methods for identifying a dengue virus serotype that involve obtaining a sample comprising at least one dengue virus protein; digesting the protein to produce peptides; ionizing the peptides; detecting a parent and associated fragment ion of the peptides; and identifying the serotype based on results of the detecting step. | 03-07-2013 |
20130059292 | METHOD OF DETECTING A TARGET USING APTAMER-MEDIATED PROTEIN PRECIPITATION ASSAY - The present invention relates to a method and detection kit for determining a presence of one or more proteins using aptamer-mediated pull-down assays. Specifically, a reproducible a protein precipitation (Co-AP/AP) method is provided to identify physiologically relevant protein-protein interactions by using target protein-specific aptamers, and to confirm its superior performance over antibody based protein precipitation (Co-IP/IP) methods in terms of its protein pull-down performance. | 03-07-2013 |
20130059293 | MAGNETIC RESONANCE SYSTEM AND METHOD TO DETECT AND CONFIRM ANALYTES - A system and method are provided to detect target analytes based on magnetic resonance measurements. Magnetic structures produce distinct magnetic field regions having a size comparable to the analyte. When the analyte is bound in those regions, magnetic resonance signals from the sample are changed, leading to detection of the analyte. | 03-07-2013 |
20130059294 | IDENTIFICATION OF POLYMORPHIC HEPATITIS B VIRUSES AND KRAS ONCOGENE MUTATIONS AND CLINICAL USE - The present application provides a method of monitoring patients of chronic hepatitis B virus (HBV) infection undergoing nucleoside/nucleotide analogue antiviral treatment for treatment efficacy and for risk of drug-resistance, by simultaneous determination of quantities of viral DNA and identification of mutant viruses responsible for drug-resistance. This invention also provides methods and reagents for highly sensitive identification/quantification of KRas oncogene mutations from body fluids or tumor tissues and the use of these methods for cancer risk assessment, cancer early detection, treatment outcome prediction, and treatment monitoring. | 03-07-2013 |
20130065220 | METHOD OF RAPIDLY DETECTING MICROORGANISMS USING NANOPARTICLES - The present invention relates to a method of rapidly detecting microorganisms using nanoparticles, and more particularly to a method and device of rapidly detecting microorganisms by adding, to the microorganisms to be detected, nanoparticles having immobilized thereon an antibody that binds specifically to the microorganisms to be detected, subjecting the mixture to an immune reaction to form a reaction solution, passing the reaction solution through a microorganism-concentrating film to concentrate the microorganisms, capturing microorganisms, which was immune-reacted with the antibody-immobilized nanoparticles, by a microorganism-capturing filtration membrane, and determining the presence and concentration of the microorganisms. | 03-14-2013 |
20130065221 | HMGB1 AND ANTI-HMGB1 ANTIBODIES FOR THE PROGNOSTIC OF NEUROLOGICAL DISORDERS - The invention relates to in vitro method for quantitating the antibodies specific for High mobility group box I (HMGB1) contained in a sample, in particular a serum sample or a cerebrospinal fluid sample obtained from a patient, and the use of this method in the prognostic and/or diagnosis of neurological disorders. These methods are in particular applicable to the monitoring of the human immunodeficiency virus (HIV) infection of a subject who is known to be infected with HIV and in the prognostic and/or diagnostic of the state of progression of Acquired immune deficiency syndrome (AIDS) or the state of progression toward AIDS, in particular the state of progression or the state of progression toward neurological disorders associated with AIDS. Finally, the invention is also about method to determine the immune deficiency or level of immune activation of a patient, in particular a HIV-infected patient. | 03-14-2013 |
20130065222 | COMPOSITIONS, METHODS AND REACTION MIXTURES FOR THE DETECTION OF MURINE LEUKEMIA VIRUS-RELATED VIRUS - The present invention relates to the detection of infectious agents, more specifically to the detection of murine leukemia viruses and other highly related viruses, including but not limited to ecotropic murine leukemia viruses, xenotropic murine leukemia viruses, and polytropic murine leukemia viruses. Compositions, methods, reaction mixtures and kits are described for the detection of MLV by using in vitro nucleic acid amplification techniques. | 03-14-2013 |
20130065223 | Universally Applicable Lysis Buffer and Processing Methods for the Lysis of Bodily Samples - The present invention provides a universally applicable lysis buffer comprising a chaotropic 5 agent, a reducing agent, and a proteolytic enzyme suitable for processing a wide variety o different sample types, such as different types of bodily samples relevant for the diagnosis o a respiratory disease. Furthermore, the present invention provides the use of a chaotropic agent, a reducing agent, and a proteolytic enzyme for the lysis of a broad spectrum of bodily samples. Moreover, the present invention provides a method for processing bodily samples which is universally applicable to the lysis of a variety of different types of bodily samples. Furthermore, the present invention provides methods for analyzing a bodily sample or for detecting the presence of a pathogen in a bodily sample, preferably, for diagnosing a respiratory disease, such as pneumonia or tuberculosis. Preferably, these methods are universally applicable to a variety of sample types, are applicable as one-tube-processes, are 15 suitable for performance in a high-throughput setting, and are automatable. | 03-14-2013 |
20130065224 | PERSONAL GLUCOSE METERS FOR DETECTION AND QUANTIFICATION OF A BROAD RANGE OF ANALYTES - A general methodology for the development of highly sensitive and selective sensors that can achieve portable, low-cost and quantitative detection of a broad range of targets using only a personal glucose meter (PGM) is disclosed. The method uses recognition molecules that are specific for a target agent, enzymes that can convert an enzyme substrate into glucose, and PGM. Also provided are sensors, which can include a solid support to which is attached a recognition molecule that permits detection of a target agent, wherein the recognition molecule specifically binds to the target agent in the presence of the target agent but not significantly to other agents as well as an enzyme that can catalyze the conversion of a substance into glucose, wherein the enzyme is attached directly or indirectly to the recognition molecule, and wherein in the presence of the target agent the enzyme can convert the substance into glucose. The disclosed sensors can be part of a lateral flow device. Methods of using such sensors for detecting target agents are also provided. | 03-14-2013 |
20130071833 | MEANS AND METHODS FOR DISTINGUISHING FECV AND FIPV - The invention provides methods and means for distinguishing FECV and FIPV, and methods and means for determining whether FIPV is present in a sample. Further provided are primers and probes for detecting FIPV specific nucleic acid sequences encoding a spike protein, antibodies for detecting a FIPV, and an immunogenic composition and use thereof for eliciting an immune response against a feline coronavirus, preferably a FIPV. | 03-21-2013 |
20130071834 | COMPOSITIONS AND METHODS USEFUL FOR HCV INFECTION - The present invention provides compositions comprising cells that can effectively produce HCV after HCV infection, compositions for culturing the cells, methods for making the composition and methods for infecting the cells in the composition with HCV. The present invention also provides methods for assaying HCV production and methods for evaluating compounds that affect the production of HCV. | 03-21-2013 |
20130078611 | METHOD FOR DETECTING MICROORGANISMS AND A KIT THEREOF - The present invention provides a method for detecting microorganisms comprising steps of PCR, magbead, complex forming, blocking and washing and reporting, in which microorganisms, particular to HBV, can be easily detected via a time-saving and user-friendly process, with high sensitivity and stability. Furthermore, according to the method for detecting microorganisms, a kit for detecting microorganisms is also provided in the present invention, so that the detection of HBV can be achieved conveniently and easily. | 03-28-2013 |
20130078612 | METHOD FOR DETECTING MICROORGANISMS AND A KIT THEREOF - The present invention provides a method for detecting microorganisms comprising steps of PCR, magbead, complex forming, blocking and washing and reporting, in which microorganisms, particular to HBV, can be easily detected via a time-saving and user-friendly process, with high sensitivity and stability. Furthermore, according to the method for detecting microorganisms, a kit for detecting microorganisms is also provided in the present invention, so that the detection of HBV can be achieved conveniently and easily. | 03-28-2013 |
20130078613 | Selection of HCV Treatment - The present invention is based on the discovery of an association between the SNP genotype at the rs12979860 locus in the IL28B gene promoter and the probability of achieving a SVR in HC V-infected patients that are given a triple therapy treatment that contains peginterferon alfa-2a, ribavirin, and a HCV NS5B polymerase inhibitor. | 03-28-2013 |
20130078614 | PROBE FOR HPV GENOTYPE DIAGNOSIS AND ANALYSIS METHOD THEREOF - The present invention relates to a probe for diagnosing HPV genotype and to a method of analyzing the same. | 03-28-2013 |
20130078615 | Device and Method for Detection and Quantification of Immunological Proteins, Pathogenic and Microbial Agents and Cells - The present invention provides a method and device for detecting and quantifying the concentration of magnetic-responsive micro-beads dispersed in a liquid sample. Also provided is a method and microfluidic immunoassay pScreen™ device for detecting and quantifying the concentration of an analyte in a sample medium by using antigen-specific antibody-coated magnetic-responsive micro-beads. The methods and devices of the present invention have broad applications for point-of-care diagnostics by allowing quantification of a large variety of analytes, such as proteins, protein fragments, antigens, antibodies, antibody fragments, peptides, RNA, RNA fragments, functionalized magnetic micro-beads specific to CD | 03-28-2013 |
20130078616 | METHOD AND SYSTEM FOR AMPLIFICATION OF NUCLEIC ACIDS IN MICROFLUIDIC VOLUME HYDROGELS - The present invention provides for a novel method and system for amplification of nucleic acids within a hydrogel of microfluidic volume using a hydrocarbon wax as a support substrate. | 03-28-2013 |
20130078617 | SAMPLE ANALYZER AND METHOD FOR CONTROLLING SAMPLE ANALYZER - A sample analyzer transports a first rack and a second rack, the first rack including a first number of supporters for supporting containers that contain biological samples of subjects, and the second rack including a second number of supporters for supporting containers that contain standard samples. The sample analyzer determines whether a transport object is the first rack or the second rack. When it has been determined that the transport object is the second rack, the sample analyzer performs a transporting operation according to the second rack and measure the standard samples in the containers supported by the second rack in a predetermined order, and prepares a calibration curve used for analyzing a measurement result of a biological sample, based on a plurality of measurement results of the standard samples. | 03-28-2013 |
20130078618 | COMPOSITIONS, REACTION MIXTURES AND METHODS FOR DETECTING NUCLEIC ACIDS FROM TYPE A1 AND/OR TYPE C1 HUMAN PAPILLOMAVIRUS - Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed. | 03-28-2013 |
20130078619 | SAMPLE PREPARATION DEVICE - A sample preparation device is disclosed. The sample preparation device includes a housing defining a passage way between a first opening and a second opening; and a sample filter occupying a section of said passage way. The sample filter contains a monolith adsorbent that specifically binds to nucleic acids. Also disclosed are sample filters containing glass frit is coated with an capture agent that binds specifically to an analyte of interest, sample filters containing a hydrophilic matrix with impregnated chemicals that lyses cell membranes, a cartridge base and an integrated sample preparation cartridge. | 03-28-2013 |
20130078620 | DEVICE AND METHOD FOR DETECTION AND IDENTIFICATION OF IMMUNOLOGICAL PROTEINS, PATHOGENIC AND MICROBIAL AGENTS AND CELLS - The present invention provides a method and device for detecting and quantifying the concentration of magnetic-responsive micro-beads dispersed in a liquid sample. Also provided is a method and microfluidic immunoassay pScreen™ device for detecting and quantifying the concentration of an analyte in a sample medium by using antigen-specific antibody-coated magnetic-responsive micro-beads. The methods and devices of the present invention have broad applications for point-of-care diagnostics by allowing quantification of a large variety of analytes, such as proteins, protein fragments, antigens, antibodies, antibody fragments, peptides, RNA, RNA fragments, functionalized magnetic micro-beads specific to CD | 03-28-2013 |
20130084560 | ANALYSIS OF A MICRONEUTRALIZATION ASSAY USING CURVE-FITTING CONSTRAINTS - A method and apparatus are disclosed for analyzing a microneutralization assay. Specifically, an automated process can be used to read the optical density of multiple samples in a microneutralization assay and plot the results using one or more constraints. A particular constraint that can be used is a maximum optical density that is read from a sample. Using the plotted curve, a neutralization titer is determined, which is the highest dilution at which a virus is effectively blocked. Other constraints can also be used. For example, a constraint can be based on using a cell control optical density as a lower asymptote and a virus control optical density as an upper asymptote. When multiple constraints are used, analysis is performed to determine which constraint provided the most accurate curve fit. | 04-04-2013 |
20130089853 | FLUORESCENT DYES - Provided are various compounds comprising the formula | 04-11-2013 |
20130089854 | KIT FOR DOT IMMUNOGOLD DIRECTED FILTRATION ASSAY AND USE THEREOF - A kit for dot immunogold directed filtration assay including a dot immunogold directed filtration card, a detection probe labeled by nano colloidal gold or latex beads, a negative standard, a positive standard, and a cleaning solution. | 04-11-2013 |
20130089855 | METHOD OF CHARACTERIZING VASCULAR DISEASES - Embodiments of the invention relate generally to methods of diagnosing diseases and measuring homeostatic states. In particular, the methods described here are used to characterize RNA from vesicles for expression of disease related markers. Embodiments of the invention also relate generally to the characterization of RNA by using sensitive techniques such as PCR to internally sample organ health using whole blood. | 04-11-2013 |
20130095470 | ASSAY FOR DETECTION OF HUMAN PARVOVIRUS B19 NUCLEIC ACID - Nucleic acid oligomers specific for human parvovirus B19 genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus B19 nucleic acid in biological specimens is disclosed. Compositions for detecting the presence of parvovirus B19 genomic DNA in human biological specimens are disclosed. | 04-18-2013 |
20130101985 | METHODS FOR THE DETECTION OF JC POLYOMA VIRUS - Methods and compositions for determining whether a subject is at risk for PML, including subjects being treated with immunosuppressants, by determining whether the subject harbors a JCV variant with reduced binding for sialic acid relative to a normal JCV, are presented. Furthermore, combinations of JCV-VP1 sequence variations that are associated with PML and that can be used as a basis of an assay for identifying subjects susceptible to PML, subjects with PML (e.g., early stage PML), or subjects at risk of developing PML in response to an immunosuppressive treatment are provided. | 04-25-2013 |
20130101986 | In Vitro Method for obtaining Intrahepatic Fibroblasts Infected with Hepatitis C Virus - The present invention relates to in vitro methods for obtaining intrahepatic fibroblasts infected with hepatitis C virus, to the infected intrahepatic fibroblasts obtained by means of these methods, and also to methods for screening for anti-fibrogenesis molecules and for anti-HCV molecules using these cells. | 04-25-2013 |
20130101987 | Detection of feline Immunodeficiency Virus - Method, device and kit for the detection of antibodies directed to Feline Immunodeficiency Virus (FIV). The method includes contacting the felid biological sample with FIV env polypeptide and detecting whether the polypeptide substantially binds to the antibody in the biological sample. The method will detect FIV antibodies in a sample from animals that have been naturally infected but the method will not detect antibodies in a sample from animals that have not been infected and that have not been vaccinated with an FIV vaccine after within about the previous five to eight weeks. | 04-25-2013 |
20130101988 | COMPOSITIONS, METHODS AND KITS TO DETECT HERPES SIMPLEX VIRUS NUCLEIC ACIDS - The disclosed invention is related to methods, compositions, kits and isolated nucleic acid sequences for targeting Herpes Simplex Virus (HSV) nucleic acid (eg. HSV-1 and/or HSV-2 nucleic acid). Compositions include amplification oligomers, detection probe oligomers and/or target capture oligomers. Kits and methods comprise at least one of these oligomers. | 04-25-2013 |
20130101989 | Diagnostic Method for Predicting the Response of a Patient to Chemovirotherapy or Radiovirotherapy - Described is a diagnostic method for predicting the response of a patient to chemovirotherapy or radiovirotherapy, comprising exposing primary tumor cells from a patient, e.g., tumor cells obtained from a brain tumor or pancreatic cancer, to (i) a parvovirus and/or (ii) a chemotherapeutic agent or radiotherapy, and determining the reduction of the expression or concentration of ISG15. | 04-25-2013 |
20130101990 | MICROFLUIDIC SYSTEM FOR AMPLIFYING AND DETECTING POLYNUCLEOTIDES IN PARALLEL - The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously. | 04-25-2013 |
20130101991 | MICROFLUIDIC ASSAY IN IDEALIZED MICROVASCULAR NETWORK FOR SELECTION AND OPTIMIZATION OF DRUG DELIVERY VEHICLES TO SIMULATED TUMORS - An apparatus for assaying a tumor drug delivery vehicle and or drug can include an idealized microvascular network (IMN) of one or more interconnected idealized flow channels in fluid communication through a porous wall with a tissue space (e.g., idealized tissue space) containing animal cells and means for quantifying drug delivery through the IMN to the animal cells. | 04-25-2013 |
20130101992 | Diagnostic Assay For Type 1 Diabetes - There is an association between enteroviruses and type 1 diabetes (T1D). The present invention is based on the finding that particular enterovirus serotypes are T1D risk serotypes, while others are protective against the disease. The present invention relates to a diagnostic assay for type 1 diabetes (T1D), and especially to an assay for predicting the risk of contracting the disease. It also relates to a method of monitoring the efficacy of antiviral treatments aiming at prevention of T1D. The invention still further relates to a diagnostic kit. | 04-25-2013 |
20130101993 | MICROCHEMICAL CHIP, PRODUCING METHOD THEREOF AND METHOD FOR USING THE MICROCHEMICAL CHIP - According to the microchemical chip of the present invention, the sample introducing port | 04-25-2013 |
20130109009 | BIOLOGICAL SAMPLE PREPARATION | 05-02-2013 |
20130109010 | METHOD FOR DETECTION OF INFECTION WITH HUMAN CYTOMEGALOVIRUS | 05-02-2013 |
20130115589 | Pharmaceutical Composition for Treatment and Prevention of Herpes Virus Infections - An object of the present invention is to find a protein expressed in a variety of cells and functioning as a receptor for herpesvirus and provide a preventive or remedy for herpesvirus infections capable of inhibiting binding of the receptor to herpesvirus and thereby preventing entry of the virus to cells. | 05-09-2013 |
20130115590 | MATERIALS AND METHOD FOR IMMOBILIZING, ISOLATING, AND CONCENTRATING CELLS USING CARBOXYLATED SURFACES - The present disclosure relates to immobilization of a cell using a carboxylated surface by contacting the carboxylated surface with a sample comprising the cell for a sufficient time to permit the cell to bind to the carboxylated surface. The immobilized cell may then be separated from the remainder of the sample and further manipulated to isolate, concentrate, and/or analyze the cell or a component thereof. | 05-09-2013 |
20130115591 | MOLECULAR METHOD FOR UNIVERSAL DETECTION OF CITRUS VIROIDS - The present invention provides methods for universally detecting | 05-09-2013 |
20130115592 | MODIFIED HUMAN HEPATITIS C VIRUS GENOMIC RNA THAT CAN BE AUTONOMOUSLY REPLICATED - The present invention provides modified hepatitis C virus genomic RNA, comprising nucleotide sequences of genomic RNA portions of two or more types of hepatitis C viruses, which comprises a 5′ untranslated region, a core protein coding sequence, an E1 protein coding sequence, a p7 protein coding sequence, an E2 protein coding sequence, an NS2 protein coding sequence, an NS3 protein coding sequence, an NS4A protein coding sequence, an NS4B protein coding sequence, an NS5A protein coding sequence, an NS5B protein coding sequence, and a 3′ untranslated region, and which can be autonomously replicated. In particular, the present invention relates to modified hepatitis C virus genomic RNA, which can be autonomously replicated by substitution of the RNA sequence portion encoding NS3, NS4, NS5A, and NS5B proteins of hepatitis C virus genomic RNA with a partial RNA sequence encoding NS3, NS4, NS5A, and NS5B proteins of a JFH1 strain shown in SEQ ID NO: 1. | 05-09-2013 |
20130115593 | SUBSTRATES FOR CHROMOGENIC DETECTION AND METHODS OF USE IN DETECTION ASSAYS AND KITS - Embodiments of substrates and processes for chromogenic detection, and in particular pyrazolyl dihydrogen phosphate compounds, are disclosed. | 05-09-2013 |
20130122484 | DIAGNOSTIC METHOD FOR DETERMINING ANIMALS PERSISTENTLY INFECTED (PI) WITH BOVINE VIRAL DIARRHEA VIRUS (BVDV) - The present specification relates to methods and kits for detection of animals that are persistently infected (PI) with a Bovine Viral Diarrhea Virus (BVDV) and/or transiently infected (TI) with BVDV. Some embodiments describe methods to distinguish a PI animal from a TI animal using a single one-time testing protocol. | 05-16-2013 |
20130122485 | METHOD OF ANALYZING BIOMATERIALS USING A MAGNETIC BEAD - Provided are methods of analyzing biomaterials using a magnetic bead. The method may include preparing a bio material including a target material, preparing first and second magnetic beads, the second magnetic bead having a size smaller than that of the first magnetic bead, forming a binding element including the target material bound on the first and second magnetic beads, separating the first magnetic bead from the binding element by using a magnet, and quantifying the target material bound on the second magnetic bead. | 05-16-2013 |
20130122486 | Compositions Comprising Human Embryonic Stem Cells and Their Derivatives, Methods of Use, and Methods of Preparation - The present invention relates to a pharmaceutical composition comprising of preparations of human embryonic stem (hES) cells and their derivatives and methods for their transplantation into the human body, wherein transplantation results in the clinical reversal of symptoms, cure, stabilization or arrest of degeneration of a wide variety of presently incurable and terminal medical conditions, diseases and disorders. The invention further relates to novel processes of preparing novel stem cell lines which are free of animal products, feeder cells, growth factors, leukaemia inhibitory factor, supplementary mineral combinations, amino acid supplements, vitamin supplements, fibroblast growth factor, membrane associated steel factor, soluble steel factor and conditioned media. This invention further relates to the isolation, culture, maintenance, expansion, differentiation, storage, and preservation of such stem cells. | 05-16-2013 |
20130122487 | DECREASING POTENTIAL IATROGENIC RISKS ASSOCIATED WITH INFLUENZA VACCINES - Influenza viruses for use in preparing human vaccines have traditionally been grown on embryonated hen eggs, although more modern techniques grow the virus in mammalian cell culture e.g. on Vero, MDCK or PER.C6 cell lines. The inventor has realised that the conditions used for influenza virus culture can increase the risk that pathogens other than influenza virus may grow in the cell lines and have identified specific contamination risks. Suitable tests can thus be performed during manufacture in order to ensure safety and avoid iatrogenic infections. | 05-16-2013 |
20130122488 | METHOD OF DETECTING SPARSE PARTICLES IN A SOLUTION USING A LIGHT-EMITTING PROBE - There is provided an optical analysis technique enabling the detection of the condition or characteristic of a particle to be observed contained at a low concentration or number density in a sample solution using a light-emitting probe. The inventive optical analysis technique uses an optical system capable of detecting light from a micro region in a solution, such as an optical system of a confocal microscope or a multiphoton microscope, to detect the light from the light-emitting probe having bound to a particle to be observed while moving the position of the micro region in the sample solution (while scanning the inside of the sample solution with the micro region), thereby detecting individually the particle crossing the inside of the micro region to enable the counting of the particle(s) or the acquisition of the information on the concentration or number density of the particle. | 05-16-2013 |
20130130231 | Bioinformatically detectable group of novel viral regulatory genes and uses thereof - The present invention relates to a group of novel viral RNA regulatory genes, here identified as “viral genomic address messenger genes” or “VGAM genes”, and as “genomic address genes” or “GR” genes. VGAM genes selectively inhibit translation of known host target genes, and are believed to represent a novel pervasive viral attack mechanism. GR genes encode an operon-like cluster of VGAM genes. VGAM and viral GR genes may therefore be useful in diagnosing, preventing and treating viral disease. Several nucleic acid molecules are provided respectively encoding several VGAM genes, as are vectors and probes both comprising the nucleic acid molecules, and methods and systems for detecting VGAM genes, and for counteracting their activity. | 05-23-2013 |
20130130232 | SELF-LOADING MICROFLUIDIC DEVICE AND METHODS OF USE - Microfluidic devices and methods for conducting chemical assays and biological assays using microfluidic devices are disclosed. The microfluidic devices do not require external connections, tethers, tubing, valves and actuators. The microfluidic devices are useful in methods for analyzing a wide variety of chemical and biological assays such as, for example, molecule-molecule interactions, enzyme-substrate interactions, molecule identification, minimum inhibitory concentrations, therapeutically effective amounts, and toxic amounts. | 05-23-2013 |
20130130233 | MONOCLONAL ANTIBODIES TO HUMAN IMMUNODEFICIENCY VIRUS AND USES THEREOF - The present invention relates to novel monoclonal antibodies which may be used in the detection of Human Immunodeficiency Virus (HIV). These antibodies exhibit an unusually high degree of sensitivity, a remarkably broad range of specificity, and bind to novel shared, non-cross-reactive epitopes. In particular, the monoclonal antibodies of the present invention may be utilized to detect HIV-1 antigen and HIV-2 core antigen in a patient sample. | 05-23-2013 |
20130130234 | POST PROTEIN HYDROLYSIS REMOVAL OF A POTENT RIBONUCLEASE INHIBITOR AND THE ENZYMATIC CAPTURE OF DNA - The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials and methods for removing aurintricarboxylic acid (ATA) from a sample. | 05-23-2013 |
20130130235 | PROBES AND PRIMERS FOR DETECTION OF DENGUE - The present disclosure gives description of a method used for the detection and quantification of dengue viral infection caused by dengue virus using nucleic acids isolated from blood, plasma or serum samples by employing Oligonucleotide probes. The method employed here for detection is by Real time PCR. The instant disclosure also provides for primers, probes, PCR Reaction mixture and kit thereof. | 05-23-2013 |
20130137082 | BIOSENSOR, APPARATUS AND METHOD FOR DETECTING A BIOMOLECULE USING THE BIOSENSOR - Provided are a biosensor, an apparatus and a method for detecting a biomolecule using the biosensor. The biosensor may include a supporting substrate, a semiconductor layer spaced apart from a top surface of the supporting substrate by supporting patterns, and a nano-motor array formed on a top surface of the semiconductor layer. The nano-motor array may include a plurality of nano-metal rods configured to exhibit an autonomous propulsion in a fluid. | 05-30-2013 |
20130137083 | Detection of H5N1 Influenza Infection - A combination of H5N1 influenza peptides that provide for H5N1 diagnosis with a high level of sensitivity and specificity is described. | 05-30-2013 |
20130137084 | Single Nucleotide Polymorphism on Chromosome 15 That Predicts HCV Treatment Responses - The present invention is based on the discovery of associations that exist between single nucleotide polymorphisms (SNPs) on chromosome 15 and virological outcomes in a diverse population of patients with hepatitis C virus (HCV) who received interferon-based treatment. | 05-30-2013 |
20130137085 | Method for Real-Time Measurement of the Individual Secretions of a Cell - The present invention relates to a method for real-time measurement of the secretion of at least one compound by at least one individual cell, comprising:
| 05-30-2013 |
20130137086 | HPV DNA Methylation Patterns of Diagnostic or Prognostic Significance in Cervical Cancer Screening - Disclosed are methods, compositions, devices, and systems for assessing cancer potential, state, stage, risk of progression, prognosis, etc. of a subject based on determining the methylation state of human papillomavirus (HPV) in a sample from the subject. The cancers assessed generally can be cancer associated with or caused by HPV. For example, cervical cancer, vulvar cancer, penile cancer, anal cancer, and head and neck cancer can be associated with HPV. It has been discovered that certain patterns, profiles, and sets of methylation of HPV genomes are correlated with different cancer potential, state, stage, risk of progression, prognosis, etc. | 05-30-2013 |
20130137087 | SYSTEM AND METHOD INCLUDING ANALYTICAL UNITS - Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples. | 05-30-2013 |
20130137088 | FACTOR INVOLVED IN LATENT INFECTION WITH HERPESVIRUS, AND USE THEREOF - Disclosed are a protein and a gene each of which is a factor involved in latent infection with a herpesvirus. An antibody against the factor was detected in approximately 50% of patients suffering from mental disorders, whereas the antibody was hardly detected in healthy persons. Further, a mouse having SITH-1 introduced therein developed a mental disorder such as a manic-depressive illness or depression-like disorder. Based on these findings, it is possible to provide a method for objectively determining a mental disorder and an animal model of a mental disorder. | 05-30-2013 |
20130137089 | HUMAN GASTROINTESTINAL STEM CELL-DERIVED PRIMARY INTESTINAL EPITHELIAL CELL SYSTEM AND METHODS OF USE THEREOF - The present invention relates to an intestinal primary epithelial cell system for detecting gastrointestinal segment-specific activation or suppression of a Toll-like receptor (TLR) by a target agent. The cell system includes an isolated human intestinal primary epithelial cell (HIPEC) line that expresses at least one TLR, where the HIPEC line is derived from a differentiable adult human gastrointestinal stem cell (ahGISC) line. Also disclosed are various methods of using the cell system, a kit that includes the cell system, and an isolated cell culture including an isolated HIPEC line derived from a differentiable ahGISC line. | 05-30-2013 |
20130137090 | NUCLEIC ACID APTAMER-BASED DIAGNOSTIC METHODS WITH NOVEL TECHNIQUES FOR SIGNAL ENHANCEMENT - The present invention concerns methods for the detection of target molecules in a sample including several steps of signal amplification allowing the detection of a very low number of target molecules in the tested sample. The detection assay is based on the use of a universal probe which enables the signal amplification. The specific recognition of the target molecule is achieved by using a specific binding agent, preferably an aptamer. The invention further concerns kits and methods for the diagnosis of pathological conditions. | 05-30-2013 |
20130143202 | COMPOSITIONS AND METHODS FOR THE DETECTION OF HIV-1/HIV-2 INFECTION - This invention relates to compositions and methods or the detection of immunodeficiency virus infection, especially immunodeficiency virus-1 (HIV-1) infection. The invention particularly concerns compositions and methods that may be used in HIV vaccine recipients whose sera may contain vaccine-generated anti-HIV-1 antibodies. | 06-06-2013 |
20130143203 | COMPOSITIONS FOR USE IN IDENTIFICATION OF ADVENTITIOUS VIRUSES - The present invention provides compositions, kits and methods for rapid identification and quantification of adventitious contaminant viruses by molecular mass and base composition analysis. | 06-06-2013 |
20130143204 | DETERMINATION OF IN VIVO DNA DOUBLE-STRAND BREAK LOCALIZATION AND APPLICATION THEREOF - The present invention relates to a method for determining the in vivo localization of double-strand breaks in a host cell, comprising incubating a host cell suspected to comprise DNA double-strand breaks and a linear polynucleotide comprising a known sequence, detecting the in vivo insertion sites of said polynucleotide in the genome of said host cell, and assessing the in vivo localization of double-strand breaks. Further envisaged by the present invention is a method for obtaining an endonuclease with altered in vivo specificity. Finally, the present invention is directed to a kit for determining in vivo specificity of an endonuclease. | 06-06-2013 |
20130143205 | HIGHLY SENSITIVE METHOD FOR DETECTION OF VIRAL HIV DNA REMAINING AFTER ANTIRETROVIRAL THERAPY OF AIDS PATIENTS - Methods for detecting polynucleotides, especially the DNA replicated from samples obtained from subjects infected with pathogenic viruses such as human immunodefiency virus, by detecting electromagnetic signals (“EMS”) emitted by such polynucleotides, and methods for improving the sensitivity of the polymerase chain reaction (“PCR”). | 06-06-2013 |
20130149694 | METHODS, DEVICES, KITS AND COMPOSITIONS FOR DETECTING ROUNDWORM, WHIPWORM, AND HOOKWORM - Methods, devices, kits and compositions for detecting the presence or absence of one or more helminthic coproantigens in a sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm, whipworm and/or hookworm in a fecal sample from a mammal and may also be able to distinguish between one or more helminth infections. Confirmation of the presence or absence of roundworm, whipworm and/or hookworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated. | 06-13-2013 |
20130149695 | METHOD FOR DETECTING GENETIC MUTATION BY USING A BLOCKING PRIMER - The present invention provides a method for detecting a gene mutation, comprising the step of performing PCR using generic PCR primers together with a blocking primer which competes with the generic primers and was modified at one end, and a method of diagnosing gene mutation-related diseases using the same. According to the invention, detection sensitivity and specificity can be increased by blocking the amplification of normal DNA and selectively amplifying mutant DNA. | 06-13-2013 |
20130149696 | HIGH-THROUGHPUT METHOD FOR DETERMINING THE PRESENCE OF PAPILLOMAVIRUS-NEUTRALIZING ANTIBODIES IN A SAMPLE - The present invention relates to a method for the determination of the presence of PV-neutralizing antibodies in a sample, comprising a) contacting said sample with infectious PV particles comprising a reporter gene, wherein the gene product of said reporter gene is secreted into the growth medium, b) contacting the PV particles from a) with host cells, and c) determining PV-neutralizing antibodies based on the amount of gene product from said reporter gene, wherein, preferably, a lower amount of said gene product as compared to a reference amount is indicative of the presence of PV-neutralizing antibodies. It further relates to a host cell strongly adhering to multi-cluster plates for use in a method for diagnosing anti-PV immunity comprising the method of the present invention. | 06-13-2013 |
20130149697 | DETECTION OF HPV - The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container. | 06-13-2013 |
20130157253 | DETECTION OF CYTOMEGALOVIRUS DNA USING AMPLIFICATION FROM BLOOD SAMPLES - Described are methods and kits for detecting cytomegalovirus DNA in liquid and dried blood samples. Primer and probe combinations for CMV detection are described as well as methods for isolating DNA from blood samples. | 06-20-2013 |
20130157254 | Method and apparatus for two-step surface-enhanced raman spectroscopy - A SERS method and apparatus employ a sample device having support structure including a first material containing a SER-active metal functionalized with a binding agent having specific capability for binding a designated target analyte. An analyte sample is introduced upon the functionalized SER-active metal; conditions to effect binding of the target analyte to the binding agent are maintained; unbound chemicals, biochemicals, or biologicals are removed; a second SER-active material is introduced to cause it to attach to the bound target analyte; the support structure is irradiated to generate a SER spectrum, with the first and second SER-active materials acting in concert; and the SER spectrum is detected and analyzed to determine the presence and quantity of the target analyte. Alternatively, the second SER-active material may be functionalized with a binding agent, with the procedure being modified accordingly. | 06-20-2013 |
20130157255 | METHOD FOR SIMULTANEOUSLY DETECTING AN ANTIGEN OF, AND AN ANTIBODY AGAINST, AN INFECTIOUS MICROORGANISM - The invention relates to a method for detecting, in vitro, an infection with a microorganism, such as the hepatitis C virus, in a biological sample, by simultaneously detecting an antigen of this microorganism and the antibodies against this same antigen, and also to the reagents and kits implementing this method. | 06-20-2013 |
20130157256 | Method for Determining the Presence of an Analyte by Means of Small Magnetic Particles, and Corresponding Device - The invention relates to a method for determining the presence of an analyte by means of a distribution of small magnetic particles. According to said method, the magnetisations of the small particles are oriented in relation to each other by means of an outer magnetic focusing field; once the focussing field has been terminated, the magnetisations of the small particles are rotated asynchronously to the magnetic field by means of an outer magnetic field of suitable field intensity and rotational frequency, which rotates about a longitudinal axis (z); the temporal course of the superpositioned transverse magnetisation of the set of particles is detected; and the presence of the analyte is deduced from the detected temporal course. The invention also relates to a corresponding device ( | 06-20-2013 |
20130157257 | Compositions Comprising the NC2 Domain of Collagen IX and Methods of Using Same - The present invention relates to the newly identified timerization initiating and stagger determining capacity of the NC2 domain of collagen IX. The invention further relates to a hexavalent molecular building block wherein the linkage of additional moieties to the amino and carboxyl terminals of monomers comprising the NC2 domain of collagen IX promotes the directed association of those moieties via the trimerization initiating and stagger determining capacity of the NC2 domain of collagen IX. | 06-20-2013 |
20130157258 | HCV NS5B PROTEASE MUTANTS - The invention provides polypeptides comprising an amino acid sequence comprising at least one variation from wild-type HCV NS5B polymerase, the at least one variation selected from the group consisting of cysteine, isoleucine, valine, or proline at amino acid position 419; alanine, valine, or asparagine at amino acid position 482; valine, isoleucine, threonine, or serine at amino acid position 486; and isoleucine at amino acid position 494, as the amino acid positions are defined in SEQ ID NO: 1, and having Hepatitis C Virus (HCV) NS5B polymerase activity. Polynucleotides encoding the polypeptide, antibodies, host cells, compositions, and methods for detecting an HCV NS5B polymerase having resistance to a polymerase inhibitor also are provided. | 06-20-2013 |
20130157259 | METHOD OF AMPLIFYING DNA FROM RNA IN SAMPLE AND USE THEREOF - Provided are methods of efficiently amplifying DNA from RNA in sample, methods of efficiently estimating an amount of RNA in a sample, and compositions for efficiently amplifying DNA from RNA in a sample. | 06-20-2013 |
20130164733 | HEPATITIS-C VIRUS TESTING - New styles of hepatitis C virus (HCV), referred to as HCV-3 and HCV-4, have been identified and sequenced. Antigenic regions of HCV-2, HCV-3 and HCV-4 polypeptides have been identified. Immunoassays for HCV and antibodies thereto are described, which allow more complete screening of blood samples for HCV, and allow HCV genotyping. | 06-27-2013 |
20130164734 | METHODS AND MATERIALS FOR THE DETECTION OF DENGUE VIRUS INFECTION - The present invention provides monoclonal antibodies that are specific for the Dengue non-structural glycoprotein NS1 in monomeric and/or oligomeric (primarily dimeric) form, together with methods, including ELISA and lateral flow assays, that employ the disclosed antibodies for the early detection of Dengue virus infection. Diagnostic kits for the detection of Dengue infection are also provided, such kits including the disclosed monoclonal and/or polyclonal antibodies. | 06-27-2013 |
20130164735 | METHOD OF MEASURING HUMAN CYP3A INDUCIBILITY - A method for measuring human CYP3A inducibility upon administration of a test drug, characterized in that a non-human animal to which a test drug is administered or a population of human cells cultured in a medium containing a test drug is infected with viruses (A) and (B); virus (A) being an adenovirus which is used as a vector and engineered by incorporating thereto a detectable reporter gene and at least 3 human PXR binding regions falling within an untranslated region of a human CYP3A gene, and virus (B) being an adenovirus which is used as a vector and engineered by incorporating thereto a human PXR cDNA; and subsequently expression level of the reporter gene is determined in the non-human animal or the cultured human cells. | 06-27-2013 |
20130164736 | METHOD FOR DETECTING MOLECULAR INTERACTIONS - The present invention relates to a method for detecting molecular interactions in a solution. In particular, the present invention relates to a method for detecting interactions between two substances that are likely to interact with one another. The present invention can be used in particular in the field of scientific research and in the field of medical analysis. | 06-27-2013 |
20130164737 | Method for Separating Target Molecules or Particles from Fibrinogen-Containing Samples Including Blood Components - A method for separating target molecules or particles from a fibrinogen containing sample comprises: (a) trapping the said target molecules or particles in a fibrin network by converting at least partially the fibrinogen contained in the sample into fibrin; (b) retracting the said fibrin network to form a fibrin clot; (c) separating the said fibrin clot from the surrounding sample medium. | 06-27-2013 |
20130171618 | POROUS MEMBRANES HAVING A POLYMERIC COATING AND METHODS FOR THEIR PREPARATION AND USE - A modified porous membrane comprising a polymer coating grafted to a porous membrane is described. The polymer coatings grafted to the porous membranes generally comprise a polymer of variable length of an electron beam (e-beam) reactive moiety, designated “poly-(A) | 07-04-2013 |
20130171619 | POROUS MEMBRANES HAVING A HYDROPHILIC COATING AND METHODS FOR THEIR PREPARATION AND USE - A modified porous membrane comprising a polymeric hydrophilic coating grafted to a porous membrane is described. The polymeric hydrophilic coatings grafted to the porous membranes comprise, for example, a PEG moiety such as a PEGMA, a PEGDA, or a TMPET, wherein the polymeric hydrophilic coating on the porous membrane decreases non-specific binding of unwanted material to the porous membrane and increases the signal to noise ratio in immunoassays, in vitro diagnostic tests, and point of care tests. Methods of making these modified porous membranes are also disclosed. | 07-04-2013 |
20130171620 | VIRAL VARIANTS AND METHODS FOR DETECTING SAME - The present invention relates generally to viral variants exhibiting reduced interactivity with immunological reagents. More particularly, the present invention is directed to hepatitis B variants exhibiting complete or partial resistance to nucleoside analogs and/or reduced interactivity with antibodies to viral surface components. The present invention further contemplates assays for detecting such viral variants which assays are useful in monitoring anti-viral therapeutic agents. | 07-04-2013 |
20130171621 | METHODS OF IN SITU DETECTION OF NUCLEIC ACIDS - Methods of detecting the presence or absence of a class of nucleic acid targets in single cells through direct or indirect capture of labels to the nucleic acids are provided, where such labels to the class of nucleic acid targets are indistinguishable from each other. Also described are methods of detecting individual cells, particularly a cell of a specific type from large heterogeneous cell populations, through detection of one or more of nucleic acid targets, where the labels to the one or more of nucleic acid targets are indistinguishable from each other. Related kits are also described. | 07-04-2013 |
20130171622 | COMPOSITIONS AND METHODS FOR DETECTING VIRAL INFECTION USING DIRECT-LABEL FLUORESCENCE IN SITU HYBRIDIZATION - The present invention relates generally to assays for the detection of viral infection and/or prognosis of viral infection and associated disease states. In particular, the invention relates to directly labeled viral-related nucleic acids having significant diagnostic, prognostic, and screening utilities and methods of using the same. | 07-04-2013 |
20130171623 | Binding Assays Utilizing Time-Resolved Up-Converting Luminescence Detection - This invention describes a general binding assay method to detect the presence and quantity of analyte in a sample. The method uses time-resolved up-converting fluorescence detection technique to provide highly sensitive detection without using expensive optical components such as band-pass optical filters. The method uses pulsed long wavelength light for excitation and time-delayed luminescence detection, resulting in little interferences from sample matrices. Furthermore, the usage of long wavelength excitation light requires simpler sample preparation and processing such as removal of red blood cells, which otherwise will significantly interfere with excitation efficiency of the luminescence probes. | 07-04-2013 |
20130171624 | Magnetic Binding Assays Utilizing Time-Resolved Up-Converting Luminescence Detection - This invention describes a general magnetic binding assay method to detect the presence and quantity of analyte in a sample. The method uses magnetic particles for separating and concentrating analytes of interest from complex samples and use time-resolved up-converting fluorescence detection technique to provide highly sensitive detection without using expensive optical components such as band-pass filters. The method uses pulsed long wavelength light for excitation and time-delayed luminescence detection, resulting in little interferences from sample matrices. Furthermore, the usage of long wavelength excitation light requires simpler sample preparation and clean-up such as removal of red blood cells, which otherwise will significantly interfere with excitation efficiency of the fluorescence probes. | 07-04-2013 |
20130171625 | BIOCHIPS AND RELATED AUTOMATED ANALYZERS AND METHODS - The present invention provides biochips that include: (a) a plurality of cards, each card having a plurality of card apertures extending therethrough, each respective card aperture having one or more cross sectional areas; and (b) a plurality of gaskets, at least one gasket residing intermediate two neighboring cards, each gasket having a plurality of gasket apertures extending therethrough. At least some of the gasket apertures have an area that is greater than that of at least one adjacent card aperture. Different sets of the gasket apertures and card apertures define a plurality of fluidic flow channels. Also provided herein are methods of making and using biochips. | 07-04-2013 |
20130183657 | MATERIALS AND METHOD FOR DETECTING CYTOMEGALOVIRUS (CMV) - Methods and kits for amplifying cytomegalovirus (CMV) nucleic acid sequences in a sample comprising pairs of primers for amplification of UL34 and UL80.5 nucleic acid sequences or one or more reagents. | 07-18-2013 |
20130183658 | Methods and Devices for Rapid Detection of Live Microorganisms by Aptamers and/or Antibodies Immobilized on Permeable Membranes - The present invention provides methods, devices and test kits for rapid detection and identification of one or more live target microorganisms in a liquid sample or grown on plates containing solid nutrient media. The invention includes mixing the one or more target microorganisms with one or more aptamers and/or one or more antibodies, each conjugated to a reporter compound and specific for a first site on the one or more target microorganisms to form a mixture. The mixture is placed on a permeable membrane having immobilized thereon one or more aptamers linked to an amine compound, and/or one or more antibodies, each specific for a second site on the one or more target microorganisms or a site on the aptamer conjugate and/or antibody conjugate. A detection solution is added to the membrane, and detection and identification of the one or more target microorganisms is achieved in less than one hour. | 07-18-2013 |
20130183659 | MICROFLUIDIC DEVICES - The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids. | 07-18-2013 |
20130183660 | Apparatus for Disease Detection - Among others, the present invention provides apparatus for detecting a disease, comprising a system delivery biological subject and a probing and detecting device, wherein the probing and detecting device includes a first micro-device and a first substrate supporting the first micro-device, the first micro-device contacts a biologic material to be detected and is capable of measuring at the microscopic level an electric, magnetic, electromagnetic, thermal, optical, acoustical, biological, chemical, physical, or mechanical property of the biologic material. | 07-18-2013 |
20130183661 | STABILIZED LEUKOCYTES AND THEIR USE IN HIV-DIAGNOSIS AND THERAPY - A method of producing extremely stable leukocytes from human blood samples which are stable for long period of time under extraordinary temperature conditions. The method also relates to producing and stabilizing the leukocytes with formaldehyde release agents. The method is directed at the use of the stabilized leukocytes as a control in assays for determining CD4+ and HIV diagnosis and therapy. | 07-18-2013 |
20130189672 | Assay Device Having Multiple Reagent Cells - An assay device includes: a liquid sample zone; a reagent zone downstream and in fluid communication with the sample zone. The reagent zone includes at least two reagent cells containing a reagent material and arranged in the reagent zone such that each reagent cell experiences substantially the same flow conditions of sample from the sample zone. The reagent cells divide the sample flow from the sample zone into multiple flow streams. Also includes are: one or more flow control elements disposed downstream from the reagent zone which combine the multiple flow streams into fewer flow streams; a detection zone in fluid communication with the reagent zone; and a wicking zone in fluid communication with the detection zone having a capacity to receive liquid sample flowing from the detection zone. The sample addition zone, the detection zone and the wicking zone define a fluid flow path. | 07-25-2013 |
20130189673 | Assay Device Having Controllable Sample Size - Disclosed is an assay device which comprises a liquid sample addition zone, a reagent zone, a detection zone, and a wicking zone, all defining a fluid flow path. The device further comprises a reagent addition zone along and in fluid communication with the fluid flow path downstream of the sample addition zone and upstream of the detection zone. An interrupting wash is added at this reagent addition zone in accordance with the method of the subject invention to control sample volume. The interrupting wash fluid is added at a predetermined fill volume on the chip device and also serves to wash the detection channel and fill the remaining chip volume. | 07-25-2013 |
20130196308 | MULTIPARAMETER ASSAY - The present invention is related to the field of pathogenic diagnostics and provides the means for typing and assessing the physical status of a pathogenic infection in a host. It is in particular directed to the determination of human papilloma virus (HPV) and the application of the assays according to the invention in monitoring the disease progression of HPV related cancer, i.e. in the differentiation between regressive and progressive HPV infected lesions. | 08-01-2013 |
20130196309 | WOVEN HYDROGEL BASED BIOSENSOR - A porous hydrogel sensor that is responsive to the presence of one or more target compounds in solution is synthesized based on demixing of certain molecules in the presence of a target compound. The porous hydrogel sensor may include fluorescently tagged antibodies that are noncovalently bound to the gel and then released in the presence of the target antigen. The porous hydrogel sensor may alternatively include dissolvable cross-links using polymerized antibody and antigen complexes so that, in the presence of the target antigen, the cross-links will be displaced and the hydrogel will dissolve. | 08-01-2013 |
20130196310 | Method and Device for Combined Detection of Viral and Bacterial Infections - A lateral flow assay is capable of detecting and differentiating viral and bacterial infections. A combined point of care diagnostic device tests markers for viral infection and markers for bacterial infection, to effectively assist in the rapid differentiation of viral and bacterial infections. In some preferred embodiments, bimodal methods and devices determine if an infection is bacterial and/or viral. A dual use two strip sample analysis device includes a first lateral flow chromatographic test strip to detect MxA and a low level of C-reactive protein and a second lateral flow chromatographic test strip to detect high levels of C-reactive protein. In some preferred embodiments, the sample is a fingerstick blood sample. | 08-01-2013 |
20130196311 | Method and Device for Combined Detection of Viral and Bacterial Infections - A lateral flow assay is capable of detecting and differentiating viral and bacterial infections. A combined point of care diagnostic device tests markers for viral infection and markers for bacterial infection, to effectively assist in the rapid differentiation of viral and bacterial infections. In some preferred embodiments, bimodal methods and devices determine if an infection is bacterial and/or viral. A dual use two strip sample analysis device includes a first lateral flow chromatographic test strip to detect MxA and a low level of C-reactive protein and a second lateral flow chromatographic test strip to detect high levels of C-reactive protein. In some preferred embodiments, the sample is a fingerstick blood sample. | 08-01-2013 |
20130203043 | PORTABLE RAPID DIAGNOSTIC TEST READER - A portable rapid diagnostic test reader system includes a mobile phone having a camera and one or more processors contained within the mobile phone and a modular housing configured to mount to the mobile phone. The modular housing including a receptacle configured to receive a sample tray holding a rapid diagnostic test. At least one illumination source is disposed in the modular housing and located on one side of the rapid diagnostic test. An optical demagnifier is disposed in the modular housing interposed between the rapid diagnostic test and the mobile phone camera. | 08-08-2013 |
20130203044 | SYSTEM, METHOD AND COMPUTER PROGRAM PRODUCT FOR THE ORGANISM-SPECIFIC DIAGNOSIS OF SEPTICEMIA IN INFANTS - A method, system, and computer program product for producing an organism specific diagnosis of septicemia in infants is disclosed. The method involves measuring the levels of one or more biomarkers against redefined threshold values and interpreting these levels to arrive at the diagnosis. Other techniques may introduce a preliminary step of identifying higher risk subjects, as well as the integration of such methods into the final diagnostic methodology. One aspect of a technique of this method may involve measuring one more cytokines to detect specific classes of infective organisms, such as Gram-negative bacteria. | 08-08-2013 |
20130203045 | METHOD FOR DETECTING NUCLEIC ACIDS BASED ON AGGREGATE FORMATION - The invention provides methods to detect or determine the presence or amount of a pathogen, such as a virus or bacterium, in a sample or the amount of cells based on the detection of their genomic DNA. The method employs magnetic substrates and subjects the sample and the magnetic substrate to forms of energy so as to induce aggregate formation and detects the aggregates. | 08-08-2013 |
20130203046 | SYSTEM AND METHOD INCLUDING ANALYTICAL UNITS - Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples. | 08-08-2013 |
20130203047 | Increasing Confidence of Allele Calls with Molecular Counting - Aspects of the present invention include methods and compositions for determining the number of individual polynucleotide molecules originating from the same genomic region of the same original sample that have been sequenced in a particular sequence analysis configuration or process. In these aspects of the invention, a degenerate base region (DBR) is attached to the starting polynucleotide molecules that are subsequently sequenced (e.g., after certain process steps are performed, e.g., amplification and/or enrichment). The number of different DBR sequences present in a sequencing run can be used to determine/estimate the number of different starting polynucleotides that have been sequenced. DBRs can be used to enhance numerous different nucleic acid sequence analysis applications, including allowing higher confidence allele call determinations in genotyping applications. | 08-08-2013 |
20130209987 | OLIGONUCLEOTIDE SETS FOR DETECTION OF HUMAN PAPILLOMAVIRUS - Disclosed are methods and kits for detecting high risk HPV genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68, that are known to cause abnormal cell growth and cancer. The disclosed methods and kits allow a rapid and quantitative real-time PCR detection of all high risk strains of HPV in a single PCR reaction. The procedure promises to facilitate the rapid high throughput detection of high risk strains of HPV in a cost effective and reliable manner. | 08-15-2013 |
20130209988 | MICROFLUIDIC DEVICES FOR THE CAPTURE OF BIOLOGICAL SAMPLE COMPONENTS - Methods and systems for selectively capturing analytes, such as cells, e.g., circulating tumor cells (CTCs), from fluid samples are disclosed. The methods include contacting the sample with an analyte binding moiety that selectively binds to the analytes; optionally separating first components of the sample including a majority of the analytes bound to the binding moieties from second components of the sample using size-based separation, e.g., in a microfluidic channel; adding to the first components of the sample a plurality of binding agents under conditions that enable a plurality of the binding agents to be linked to the analyte binding moieties to form multivalent tagging agents bound to the analyte; passing the first components of the sample past a surface to which is attached a plurality of capture agents that selectively bind to the binding agents; and capturing the analytes by providing conditions that enable the multivalent tagging agents bound to the analytes to bind to one or more of the capture agents. | 08-15-2013 |
20130209989 | OLIGONUCLEOTIDE PRIMER COMPOSITION - Oligonucleotide primer useful for synthesizing a cDNA copy of HIV-1 nucleic acids from a broad range of HIV-1 subtypes, including M group and O group variants. | 08-15-2013 |
20130209990 | DETECTION OF NUCLEIC ACIDS BY TYPE-SPECIFIC HYBRID CAPTURE METHODS - Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but is also highly specific and capable of discriminating highly homologous nucleic acid sequences. The method produces DNA/RNA hybrids which can be detected by a variety of methods. | 08-15-2013 |
20130209991 | WIRELESS SWNT SENSOR INTEGRATED WITH MICROFLUIDIC SYSTEM FOR VARIOUS LIQUID SENSING APPLICATIONS - Sensors based on single-walled carbon nanotubes (SWNT) are integrated into a microfluidic system outfitted with data processing and wireless transmission capability. The sensors combine the sensitivity, specificity, and miniature size of SWNT-based nanosensors with the flexible fluid handling power of microfluidic “lab on a chip” analytical systems. Methods of integrating the SWNT-based sensor into a microfluidic system are compatible with the delicate nature of the SWNT sensor elements. The sensor devices are capable of continuously and autonomously monitoring and analyzing liquid samples in remote locations, and are applicable to real time water quality monitoring and monitoring of fluids in living systems and environments. The sensor devices and fabrication methods of the invention constitute a platform technology, because the devices can be designed to specifically detect a large number of distinct chemical agents based on the functionalization of the SWNT. The sensors can be combined into a multiplex format that detects desired combinations of chemical agents simultaneously. | 08-15-2013 |
20130209992 | METHODS OF NONSPECIFIC TARGET CAPTURE OF NUCLEIC ACIDS - Methods for capturing a target nucleic acid from a sample by using a capture probe that binds nonspecifically to the target nucleic acid and binds specifically to an immobilized probe via a specific binding pair that has one member on the capture probe and one member on the immobilized probe are disclosed. Compositions that include a capture probe that binds nonspecifically to a target nucleic acid and specifically to an immobilized probe via binding of members of a specific binding pair in a solution phase of a reaction mixture are disclosed. | 08-15-2013 |
20130209993 | SAMPLE COLLECTION SYSTEM AND METHOD FOR USE THEREOF - A sample collection system capable of collecting, storing and dispensing a liquid sample is disclosed. The collection system includes a collector composed of a material which has the unique ability to express constituents of interest at levels which are much more concentrated than their levels in the fluid samples from which they are expressed, where the expressed highly concentrated sample can then be used with modern rapid screening/testing protocols, such as solid phase assays, to test for the constituents of interest. Thus, it is now possible to obtain analytes of interest, such as the HIV protein antibodies, from saliva samples at concentrations that are detectable with systems and/or devices that are typically utilized only for blood serum or plasma testing. The collector is sized and shaped to fit within a recovery container, which, in turn, is sized and shaped to fit within a collection tube. The recovery container includes an aperture which does not permit passage of fluid under ambient conditions, but facilitates transfer thereof when subjected to pressure. An optional channel within the collection tube facilitates dispensing of the sample for further processing. | 08-15-2013 |
20130216996 | ELECTRICALLY CONDUCTIVE POLYMER NANOWIRES WITH INCORPORATED VIRUSES - Grafting M13 bacteriophage into an array of poly(3,4-ethylenedioxythiophene) (PEDOT) nanowires generated hybrids of conducting polymers and replicable genetic packages (rgps) such as viruses. The incorporation of rgps into the polymeric backbone of PEDOT occurs during electropolymerization via lithographically patterned nanowire electrodeposition (LPNE). The resultant arrays of rgps-PEDOT nanowires enable real-time, reagent-free electrochemical biosensing of analytes in physiologically relevant buffers. | 08-22-2013 |
20130216997 | Methods and Systems for Detection of Microorganisms - Disclosed are methods and systems for the isolation and detection of microbes from a sample. The use of binding agents for isolation of a microbe of interest from a sample are described. In certain embodiments, the methods use ribosome-based and/or bacteriophage-based amplification of the signal in detection of bacteria and other microorganisms. For example, embodiments of the present invention can achieve total amplification of at least 10,000 from a single infected cell. | 08-22-2013 |
20130216998 | METHOD FOR LINKING POINT OF CARE RAPID DIAGNOSTIC TESTING RESULTS TO LABORATORY-BASED METHODS - A method for using a single sample suspected of containing a microorganism for both a local rapid test immunoassay and a remote laboratory test. The sample is collected from a patient at a physician's office or from the environment to be tested. The sample is collected using a swab or other implement, combined with a rapid test processing reagent and a portion of the processed sample is used for the local rapid test. The rapid test processing reagent typically consists of a buffer, a salt, and a detergent and is compatible with the local rapid test immunoassay. Only a portion of the processed sample is used for the local rapid test, leaving a remaining portion of the processed sample to be used in a remote laboratory assay. At least some of the remaining portion of the processed sample is combined with a stabilization agent that preserves at least the nucleic acid in the processed sample for the remote laboratory assay. | 08-22-2013 |
20130216999 | METHODS AND KITS FOR THE DETECTION OF AN INFECTION IN SUBJECTS WITH LOW SPECIFIC ANTIBODY LEVELS - This invention relates to methods that enable the detection of antibodies against a latent infection, a chronic infection, a re-infection, and/or a breakthrough infection; enable the diagnosis of a latent infection, a chronic infection, a re-infection, and/or a breakthrough infection; and increase low anti-viral antibody levels, and a kit for the detection of virus-specific antibodies expressed at low levels. | 08-22-2013 |
20130217000 | METHOD FOR CHARACTERISING A BIOLOGICALLY ACTIVE BIOCHEMICAL ELEMENT BY ANALYSING LOW FREQUENCY ELECTROMAGNETIC SIGNALS - A method for characterizing a biologically active biochemical element in a sample by prefiltering the sample and analyzing low frequency electromagnetic signals transmitted by the prefiltered solution. The prefiltering may be through a 150 nm or less filter. The prefiltering may be subsequent to a dilution, e.g., between 10 | 08-22-2013 |
20130217001 | METHOD FOR MONITORING A STERILIZATION PROCESS - The disclosed invention relates to a method for monitoring a sterilization process. The method comprises: (A) exposing an article to be sterilized and a biological indicator to a sterilization medium during a sterilization process, the biological indicator comprising a cell with a plasma membrane in which the biological indicator is positioned on part or all of an electronically conductive material positioned on a substrate; and (B) measuring the membrane potential of the cell to detect the viability of the cell. | 08-22-2013 |
20130217002 | HIV-1 IGG3 RESPONSE IN ACUTE HIV-1 - The present invention relates, in general, to HIV-1 and, in particular, to methods of detecting incident HIV-1 infection. | 08-22-2013 |
20130217003 | METHOD FOR DETERMINING AN ANALYTE CONTENT OF A LIQUID SAMPLE BY MEANS OF A BIOANALYZER - A method for automated in situ determining an analyte content of a liquid sample by means of a bioanalyzer, wherein a measurement duct has at least one substrate, comprising a repeatedly performable sequence of steps as follows: (i) preparing a sensor matrix, which has a plurality of receptors, which bind the analyte and/or a further target molecule, or bring about a chemical conversion of the analyte or of the further target molecule, leading through the measurement duct a preparation solution of at least a first chemical species, wherein a plurality of the first chemical species are bound on the substrate via the functional group binding on the substrate, wherein the other functional group of the plurality of the first chemical species bound on the substrate serves as a receptor or for subsequent binding of a receptor; (ii) leading the liquid sample, through the measurement duct, wherein analyte contained in the liquid sample or in the liquid to be measured, and/or other target molecules contained in the liquid sample or the liquid to be measured, bind, preferably selectively and specifically, on the receptors or are chemically converted by the receptors, and determining a measured variable correlated with the amount of the target molecules bound or converted by the receptors, and deriving therefrom the analyte content of the liquid sample; and (iii) regenerating, especially clearing, the at least one substrate, wherein the sensor matrix and, in given cases, molecules bound thereto, especially analyte molecules, other target molecules or other molecules, are released from the substrate and/or at least partially decomposed. | 08-22-2013 |
20130224729 | Biodetection Methods and Compositions - Diagnostic methods and compositions for detecting biological material are provided. | 08-29-2013 |
20130224730 | PEPTIDE LIGANDS - A method of solid phase selection of peptide ligands for target proteins is presented. 15-20mers or greater are addressed in a microarray, and the target protein and optional competitor bound thereto and binding compared. A specific signal for the target protein indicates that a peptide has strong affinity for the target. Ligands can be coupled to solid supports and used for affinity purification of the target proteins as well as detection and modulation of target proteins. Specific peptide ligands for immuno-purifying norovirus. | 08-29-2013 |
20130224731 | ACOUSTIC RADIATION FOR EJECTING AND MONITORING PATHOGENIC FLUIDS - The invention relates to methods and devices that use focused radiation to handle and/or monitor pathogenic fluids. In particular, a method is provided for dispensing one or more droplets of a fluid containing a pathogen. The method involves providing the pathogen-containing fluid in a reservoir and applying focused radiation to the pathogen-containing fluid in the reservoir in a manner effective to eject a droplet of the fluid therefrom. Often, a pathogen-impermeable enclosure is used. | 08-29-2013 |
20130224732 | METHOD FOR AMPLIFYING VARIATION OF FREQUENCY OF SIGNAL IN PIEZOELECTRICAL BIOSENSOR - Provided is a method for amplifying a frequency variation of a detected signal in a biosensor that is used for detecting a biomolecule by measuring a change in frequency of an oscillating signal, the change being caused by pressure a biomolecule applies to a piezoelectric substance. The method for amplifying a frequency variation of a detected signal comprises the steps of: (a) applying a sample to a probe being fixed to an upper portion of a substrate of the biosensor to allow a biomolecule in the sample to be bound to the probe; (b) applying protein tagged with a metal particle to the biosensor to allow the protein and the biomolecule to be bound with each other; and (c) applying a metal enhancer to the biosensor to allow the metal enhancer to be bound to the metal particle having been bound to the protein. | 08-29-2013 |
20130230844 | SYSTEM AND APPARATUS FOR POINT-OF-CARE DIAGNOSTICS - A system comprised of an apparatus and a test device is described. The test device and the apparatus are designed to interact to determine the presence or absence of an analyte of interest in a sample placed on the test device. | 09-05-2013 |
20130230845 | INTERACTIVE TEST DEVICE AND APPARATUS WITH TIMING MECHANISM - A system comprised of an apparatus and a test device is described. The test device and the apparatus are designed to interact to determine the presence or absence of an analyte of interest in a sample placed on the test device. The test device and apparatus interact to provide a timer feature for determining a test device specific adjustable cut-off value that is used to ascertain whether signal from a test line in the device corresponds to a positive or negative results, irrespective of the time elapsed since placement of sample on the test device. The adjustable cut-off value renders the system relatively insensitive to incubation time of the test device, where if the incubation time is shorter or longer than needed for accuracy of a test result, the analyzer will report an invalid result, thus preventing the reporting of an incorrect (false negative or false positive) result. | 09-05-2013 |
20130230846 | Multiplanar Lateral Flow Assay with Diverting Zone - A lateral flow device includes a sample compressor and a test strip comprising a diverting zone. The diverting zone, which may include a barrier and/or a gap or ditch, stops or impedes flow. Flow is reinitiated and diverted into an alternate plane by compression of a sample compressor. Flow returns to the original, lateral plane, at the end of the diverting zone. | 09-05-2013 |
20130236880 | DIRECT DETECTION OF UNAMPLIFIED HEPATITIS C VIRUS RNA USING UNMODIFIED GOLD NANOPARTICLES - A gold nanoparticle-based colorimetric assay kit for hepatitis C virus RNA that detects unamplified HCV RNA in clinical specimens using unmodified AuNPs and oligotargeter polynucleotides that bind to HCV RNA. A method for detecting hepatitis C virus comprising contacting a sample suspected of containing hepatitis C virus with a polynucleotide that binds to hepatitis C virus RNA and with gold nanoparticles, detecting the aggregation of nanoparticles, and detecting hepatitis C virus in the sample when the nanoparticles aggregate (solution color becomes blue) in comparison with a control or a negative sample not containing the virus when nanoparticles do not aggregate (solution color remains red). | 09-12-2013 |
20130236881 | THREE-DIMENSIONAL METAL-COATED NANOSTRUCTURES ON SUBSTRATE SURFACES, METHOD FOR PRODUCING SAME AND USE THEREOF - The invention relates to a method for producing column-shaped or conical nanostructures, wherein the substrate surface is covered with an arrangement of metal nanoparticles and etched, the nanoparticles acting as an etching mask and the etching parameters being set such that column structures or cone structures are created below the nanoparticles and the nanoparticles are preserved as a structural coating. | 09-12-2013 |
20130236882 | Micro-Devices for Improved Disease Detection - Among others, the present invention provides micro-devices for detecting or treating a disease, each comprising a first sorting unit capable of detecting a property of the biological subject at the microscopic level and sorting the biological subject by the detected property; a first detection unit capable of detecting the same or different property of the sorted biological subject at the microscopic level; and a first layer of material having an exterior surface and an interior surface, wherein the interior surface defines a first channel in which the biological subject flows from the first sorting unit to the first detection unit. | 09-12-2013 |
20130236883 | PROCESS OF DIRECTLY DETECTING AND IDENTIFYING A MICROORGANISM IN A BIOLOGICAL SAMPLE DILUTED IN AN ENRICHMENT BROTH - The present invention generally relates to the field of analysis for example biological analysis. More specifically, the present invention relates to a process of detecting at least one microorganism present in a sample placed in a closed container, said method comprising essentially the following steps:
| 09-12-2013 |
20130236884 | COMPOSITIONS FOR USE IN IDENTIFICATION OF ORTHOPOXVIRUSES - Oligonucleotide primers and compositions and kits containing the same for rapid identification of | 09-12-2013 |
20130244221 | METHOD FOR ISOLATING HEPATITIS A VIRUS OR SPRING VIREMIA OF CARP VIRUS - A method for isolating Hepatitis A virus or Spring viremia of Carp virus. A virus probe is prepared by linking a magnetic bead-conjugated Protein G with an anti-HAV (Hepatitis A Virus) antibody or an anti-rhabdovirus antibody. The virus probe is contacted with a sample to be analyzed to form a virus probe-virus complex. The virus probe-virus complex is then isolated. It may specifically isolate Hepatitis A virus or Spring viremia of Carp virus from a sample mixed viruses. | 09-19-2013 |
20130244222 | LIPOPARTICLES COMPRISING ION CHANNELS, METHODS OF MAKING AND USING THE SAME - The present invention relates to the use of lipoparticles, virus-like particles, and viruses. The present invention also relates to testing ion channel function and modulators of ion channels. | 09-19-2013 |
20130244223 | RESPIRATORY SYNCYTIAL VIRUS (RSV) VIRAL LOAD DETECTION ASSAY - The invention concerns a method for the extraction of nucleic acids from biological samples e.g. tissue material or sputum derived from human or animal species and the quantitative detection thereafter of said nucleic acids e.g. in terms of viral load, more specifically RSV viral load detection. | 09-19-2013 |
20130244224 | ENDOTOXIN DETECTION METHOD - The invention relates to a method for detecting endotoxins in a sample. | 09-19-2013 |
20130244225 | MICROORGANISM CONCENTRATION PROCESS AND DEVICE - A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising (1) a porous fibrous nonwoven matrix and (2) a plurality of particles of at least one concentration agent that comprises diatomaceous earth, the particles being enmeshed in the porous fibrous nonwoven matrix; (b) providing a sample comprising at least one target cellular analyte; (c) contacting the concentration device with the sample such that at least a portion of the at least one target cellular analyte is bound to or captured by the concentration device; and (d) detecting the presence of at least one bound target cellular analyte. | 09-19-2013 |
20130244226 | VIRUS DETECTION DEVICE AND VIRUS DETECTION METHOD - A virus detection device includes a diffusion unit configured to diffuse a virus in a gas as an inspection target into an aqueous solution containing a fluorescent antibody specifically adsorptive to the virus by bringing the gas into contact with the aqueous solution and configured to adsorb the fluorescent antibody to the virus in the gas; an atomization unit configured to atomize the aqueous solution and generate a mist group of the aqueous solution in which the gas is diffused; a fluorescence measuring unit configured to measure a fluorescence intensity of the mist group; and an air current generator configured to form an air current flowing toward the fluorescence measuring unit from the atomization unit. | 09-19-2013 |
20130244227 | METHOD FOR AMPLIFICATION-FREE NUCLEIC ACID DETECTION ON OPTOFLUIDIC CHIPS - An optofluidic platform is constructed so as to comprise a planar, liquid-core integrated optical waveguides for specific detection of nucleic acids. Most preferably, the optical waveguides comprises antiresonant reflecting optical waveguide (ARROWs). A liquid solution can be prepared and introduced into the optofluidic platform for optical excitation. The resulting optical signal can be collected at the edges of the optofluidic platform and can be analyzed to determine the existence of a single and/or a specific nucleic acid. | 09-19-2013 |
20130244228 | RAPID TEST FOR THE QUALITATIVE AND/OR QUANTITATIVE ANALYSIS OF ANTIBODIES AGAINST HUMAN PAPILLOMA VIRUSES (HPV) PRESENT IN BODY FLUID, AND DEVICE FOR CARRYING OUT THE RAPID TEST - A rapid test for a qualitative and/or quantitative assay of antibodies present in body fluid against human papilloma viruses (HPV) includes mixing a specimen of body fluid with a reagent which essentially comprises a predetermined quantity of physiologically acting liquid and a predetermined quantity of at least one HPV-specific antigen. The mixture is fed to an analysis which utilizes a change that is at least one of measurable or perceivable by a user. | 09-19-2013 |
20130244229 | BUFFERING COMPOSITIONS ENCLOSED IN A SIZE EXCLUSION MATRIX - The present invention relates to a buffering composition, more closely a composition comprising porous matrix-enclosed buffering agent(s) giving a stabilisation of pH when applied in for example aqueous environment. | 09-19-2013 |
20130252231 | Biologic Machines for the Detection of Biomolecules - Disclosed are methods, devices and systems for the isolation and detection of biomolecules from a sample. The embodiments, detection of such biomolecules provides for detection of microorganisms. For example, disclosed are methods, devices and systems that use bacteriophage-based amplification of the signal in detection of bacteria and other microorganisms. The devices, systems and methods of the invention may allow for the detection of certain biomolecules peptides and ions in real time using minute amounts of sample. | 09-26-2013 |
20130252232 | HEPATITIS B VIRUS TYPING AND RESISTANCE ASSAY - The present invention provides methods, kits, and oligonucleotides for detecting and analyzing the nucleotide sequence of a reverse transcriptase (RT) region of the polymerase (Pol) gene of Hepatitis B Virus (HBV). In certain embodiments, a target RT region is amplified and subjected to DNA sequencing. The sequence obtained is compared to one or more DNA sequences characteristic of an HBV genotype or serotype, and/or one or more DNA sequences characteristic of an HBV mutation that confers resistance to a drug or vaccine, to determine the HBV genotype or serotype of the amplified product and/or the presence or absence of one or more DNA sequences characteristic of an HBV mutation that confers resistance to a drug or vaccine. | 09-26-2013 |
20130252233 | PREDICTION OF ANTIVIRAL THERAPY RESPONSE - The application relates to methods for determining whether or not antiviral therapies will be effective. In particular, the present application provides a method using miRNA, e.g. miR-122 or miR-296-5p, for determining the likelihood that a subject having a viral infection of the liver will be responsive to antiviral therapy that includes stimulation of Interferon (IFN) activity, and kits for the performance of said determination. | 09-26-2013 |
20130252234 | METHODS AND DEVICES FOR ELECTRONIC SENSING - The presence of a detectable entity within a detection volume of a microfabricated elastomeric structure is sensed through a change in the electrical or magnetic environment of the detection volume. In embodiments utilizing electronic detection, an electric field is applied to the detection volume and a change in impedance, current, or combined impedance and current due to the presence of the detectable entity is measured. In embodiments utilizing magnetic detection, the magnetic properties of a magnetized detected entity alter the magnetic field of the detection volume. This changed magnetic field induces a current which can reveal the detectable entity. The change in resistance of a magnetoresistive element may also reveal the passage of a magnetized detectable entity. | 09-26-2013 |
20130260367 | NMR SYSTEMS AND METHODS FOR THE RAPID DETECTION OF ANALYTES - This invention features systems and methods for the detection of analytes, and their use in the treatment and diagnosis of disease. | 10-03-2013 |
20130260368 | Capture Probes Immobilizable Via L-Nucleotide Tail - The invention provides chimeric capture probes immobilizable via an L-nucleic acid tail that can bind to a complementary L-nucleic acid in an immobilized probe. The capture probes are useful for capturing a target nucleic acid from a sample. The L-nucleic acid in the tail of the capture probe bind to the complementary L-nucleic acid in the immobilized probe with similar affinity as would otherwise equivalent D-nucleic acids. However, the L-nucleic acid of the capture probe tail and immobilized probes do not form stable duplexes with D-nucleic acids present in the sample containing the target nucleic acid. Binding of nucleic acids in the sample directly to immobilized probe or to the tail of the capture probe is reduced or eliminated increasing the sensitivity and/or specificity of the assay. | 10-03-2013 |
20130260369 | Compositions and Methods for the Collection and Isolation of Nucleic Acids from Biological Specimens - The invention is directed to tools, compositions and methods for collecting, storing, transporting, isolating and detecting macromolecules such as nucleic acid sequences obtained from specimens. The compositions are one-step formulations for killing or inactivating pathogens, inactivating enzymes, and releasing nucleic acids from the specimens that are prepared for further processing and/or analysis. In particular, the invention provides a single, one-step, sample collection and transport formulation that facilitates the concentration, extraction, isolation and analysis of nucleic acids, genes and genomes. | 10-03-2013 |
20130260370 | MICROORGANISM CONCENTRATION PROCESS AND DEVICE - A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising (1) a porous fibrous nonwoven matrix and (2) a plurality of particles of at least one concentration agent that comprises a metal silicate, the particles being enmeshed in the porous fibrous nonwoven matrix; (b) providing a sample comprising at least one target cellular analyte; (c) contacting the concentration device with the sample such that at least a portion of the at least one target cellular analyte is bound to or captured by the concentration device; and (d) detecting the presence of at least one bound target cellular analyte. | 10-03-2013 |
20130266930 | ANTIBODY-LINKED IMMUNO-SEDIMENTATION AGENT AND METHOD OF ISOLATING A TARGET FROM A SAMPLE USING SAME - The present disclosure is directed to antibody-linked immuno-sedimentation agent, the antibody being linked to a sedimentation agent by a non-antigen binding region of the antibody, and a method of isolating a target from a sample using the antibody-linked immuno-sedimentation agent. The methods involve forming a mixture including a sample with an antibody linked immuno-sedimentation agent and red blood cells under conditions sufficient to form red blood cell rouleaux and allow antibody-antigen binding. | 10-10-2013 |
20130266931 | RETROVIRUS DETECTION - Provided are methods and compositions useful for detecting viral infection or contamination in a biological sample. | 10-10-2013 |
20130266932 | METHOD AND ASSOCIATED SYSTEM FOR DETECTION AND ANALYSIS OF PATHOGENS AND/OR AGENTS ABLE TO CAUSE DETERIORATION IN PLANT FOODS - The present invention relates to a method for the detection of potentially pathogenic agents and/or capable of causing deterioration in a plant sample, representative of one or several batches of plant product, characterized in that it comprises the concentration of a plant sample by centrifugation and the analysis of the presence or amount of potentially pathogenic agents and/or capable of causing deterioration in the concentrated sample obtained. Likewise, the present invention relates to a system for detection and analysis of potentially pathogenic agents and/or capable of causing deterioration in a plant sample to carry out said method. | 10-10-2013 |
20130266933 | PROCEDURE FOR DETERMINING VARIANTS OF INFECTIOUS PANCREATIC NECROSIS VIRUS IN AQUATIC ANIMALS; ASSOCIATED DETECTION KIT; AND USE OF THE PROCEDURE IN AQUATIC ANIMALS - The present invention is related to a low cost, fast, specific, sensitive, and suitable for routine application in monitoring activities, procedure for determining variants of infectious pancreatic necrosis virus (IPNV) on samples of different origin; and associated kit. | 10-10-2013 |
20130266934 | COMPOSITIONS AND METHODS FOR IDENTIFYING AND DIFFERENTIATING VIRAL COMPONENTS OF MULTIVALENT SHIPPING FEVER VACCINES - Disclosed are methods and compositions for identifying viral-specific polynucleotide sequences in a biological sample, and particularly in samples of veterinary origin. Also disclosed are oligonucleotide primer pairs, as well as labeled oligonucleotide detection probes useful in detecting the presence of one or more particular species, strains, types, or subtypes of one or more mammalian pathogens of viral origin, as well as systems, diagnostic kits and articles of manufacture comprising virus-specific primers and labeled detection probes, including those useful in identifying viral components of a multivalent vaccine, and quantitating the potency of particular attenuated, live viruses that comprise a livestock vaccine. In certain embodiments, real-time, quantitative PCR methods have been utilized to identify and distinguish between the three known genetic subtypes of bovine viral diarrhea viruses (BVDV-1a, BVDV-1b, and BVDV-2) in a multivalent bovid shipping fever vaccine. | 10-10-2013 |
20130273520 | COMPOSITION, DEVICE, AND METHOD FOR BIOLOGICAL AIR SAMPLING - The present invention generally relates to air sampling of biological compounds. Specifically, the present invention relates to a device and method for sampling the ambient air for detecting microbial propagules, microbial propagules being any spore, vegetative cell, or virion of microbiological origin including all bacteria, fungi, viruses, protozoans, molds, slime molds, chlamydospores, hyphae, and cysts. | 10-17-2013 |
20130273521 | SIGNALLING SYSTEM - The invention concerns a system for detecting a target nucleic acid molecule of a particular sequence in a sample, said system comprising (i) an oligonucleotide primer complementary to a said target nucleic acid molecule, which primer has no internal complementarity, is able to amplify said target sequence and carries a first label linked to said oligonucleotide at its 5′ end; and (ii) an oligonucleotide probe which carries a second label that is able to interact with said first label to produce a detectable signal, wherein the oligonucleotide probe binds an extension product of said primer such that the first and second label can interact to produce a detectable signal. Methods for using said system in particular in a nucleic acid assay, kits comprising the system and elements of it form a further aspect of the invention. | 10-17-2013 |
20130273522 | NMR SYSTEMS AND METHODS FOR THE RAPID DETECTION OF ANALYTES - This invention features systems and methods for the detection of analytes, and their use in the treatment and diagnosis of disease. | 10-17-2013 |
20130273523 | NMR SYSTEMS AND METHODS FOR THE RAPID DETECTION OF ANALYTES - This invention features systems and methods for the detection of analytes, and their use in the treatment and diagnosis of disease. | 10-17-2013 |
20130273524 | DEVICE FOR PERFORMING A BLOOD, CELL, AND/OR PATHOGEN COUNT AND METHODS FOR USE THEREOF - Devices and methods for performing a point of care blood, cell, and/or pathogen count or a similar blood test. Disclosed herein are systems that can be used to provide rapid, accurate, affordable laboratory-quality testing at the point of care. The systems described herein are capable of imaging and counting individual cells in a prepared cell sample (e.g., a peripheral blood smear or a blood sample prepared in a microfluidic device) or another prepared cell-containing sample without the need for a microscope or other expensive and cumbersome optics. The systems described herein are designed to eliminate or replace expensive, centralized clinical testing equipment and technical personnel. Such systems may include automated data reporting and decision support. | 10-17-2013 |
20130273525 | MULTIPLEX METHOD FOR DETECTING AN INFECTION - The invention relates to a method for the in vitro diagnostic detection of an infection with a microorganism, comprising placing a biological sample, in a single assay receptacle, in the presence of particles, each carrying at least one specific detectable physical parameter, and belonging to at least two different groups, one of the groups carrying an anti-IgM capture antibody and the other group carrying a capture antigen derived from said microorganism. | 10-17-2013 |
20130280695 | METHOD, DEVICE AND TEST KIT FOR MOLECULAR-BIOLOGICAL REACTIONS - The invention relates to a device, method and test kit for carrying out molecular-biological reactions, wherein the different components for the molecular-biological reactions are located on a solid carrier in different, spatially separated compartments prior to the start of the reaction. The carrier is preferably a porous filter disk made of polyethylene. Fields of application are the amplification of nucleic acids, for example PCR or RealTime PCR, the reverse transcription of RNA in DNA enzyme-substrate interactions or antigen-antibody interactions, or protein synthesis. | 10-24-2013 |
20130280696 | DEVICES AND METHODS FOR DETECTING ANALYTE IN BODILY FLUID - A test device for detecting an analyte in a sample of bodily fluid, includes at least one hollow needle configured to obtain a sample of bodily fluid, an enclosure configured to contain a reagent, a portion configured to open the enclosure, a test results indicator configured to absorb the bodily fluid, the test results indicator including at least one test zone configured to indicate the presence of an analyte in the bodily fluid when the at least one test zone is in fluidic contact with the reagent, a housing, and a cover configured to be movable with respect to the housing between a closed position precluding access to the at least one hollow needle and an open position permitting access to the at least one hollow needle. Additionally, the at least one hollow needle is in fluid communication with the test results indicator. | 10-24-2013 |
20130280697 | HEV Assay - A method of simultaneously amplifying genotypes 1, 2, 3 and/or 4 of HEV is disclosed comprising amplifying the genotypes 1, 2, 3 and/or 4 of HEV with one single none-degenerate forward primer partially overlapping the 5′UTR region of HEV and at least one reverse primer. Also disclosed are related methods comprising a probe, and kits for the detection of genotypes 1, 2, 3 and/or 4 of HEV. | 10-24-2013 |
20130280698 | RAPID MULTIPLEX LATERAL FLOW ASSAY DEVICE - Disclosed herein are devices, systems and methods for simultaneously conducting multiple assays on a liquid sample for the presence of, and/or quantification of, analytes in the liquid sample. The device, which is referred to herein as a multi-assay cartridge or multi-strip assay cartridge (MSC), may be used in a system comprised of the cartridge and a reading device or reader for reading the assays in the cartridge. | 10-24-2013 |
20130280699 | ASSAY DEVICE - An assay device ( | 10-24-2013 |
20130288229 | METHOD FOR DETECTION, DIFFERENTIATION AND QUANTIFICATION OF T CELL POPULATIONS BY WAY OF REVERSE TRANSCRIPTION QUANTITATIVE REAL TIME PCR (RT-qPCR) TECHNOLOGY - The present invention relates to a method for detection, differentiation and quantification of T cell populations, comprising the following steps a) contacting a first aliquot of a body fluid of an individual with at least one antigen, wherein the body fluid contains antigen presenting cells (APC) and T cells, b) incubating the first aliquot with at least one antigen for a certain period of time, c) detection and differentiation of the T cell population by detecting in the first aliquot and in a second aliquot of the body fluid of the individual, which has not been incubated with the at least one antigen, at least a first marker of the APC induced by T cells in a specific T cell population using reverse transcription quantitative real time-time polymerase chain reaction (RT-qPCR), and d) detection and quantification of the T cell population by determining the ratio of the detected marker of the APC of the first aliquot to the second aliquot as well as a kit for performing the method. | 10-31-2013 |
20130288230 | Method for identification of a natural biopolymer - The invention presents a method of identifying natural biopolymer—a protein, DNA, RNA in biological fluids and environmental objects, which is based only on the structure of the biopolymer and does not require pathogen genome sequencing, or animals vaccination by biopolymer-antigen. For this purpose the biopolymer itself is taken—a protein, DNA, or RNA, that is fragmented with enzyme to oligomer fragments—a mixture of oligopeptides, oligonucleotides DNA mixture, mixture of RNA oligonucleotides, without dividing the mixture into individual components, then carboxylation of structure in oligomer components is performed by acylation or alkylation. | 10-31-2013 |
20130288231 | UNIVERSAL MULTI-VARIANT DETECTION SYSTEM - The present invention provides a method to diagnostically detect the variants of a given pathogen, such as HIV, hepatitis C, hepatitis B (HBV), Parvovirus B19, etc., with the use of a single detection probe. | 10-31-2013 |
20130288232 | FAST THERMO-OPTICAL PARTICLE CHARACTERISATION - The present invention relates to a method and an apparatus for a fast thermo-optical characterisation of particles. In particular, the present invention relates to a method and a device to measure the stability of (bio)molecules, the interaction of molecules, in particular biomolecules, with, e.g. further (bio)molecules, particularly modified (bio)molecules, particles, beads, and/or the determination of the length/size (e.g. hydrodynamic radius) of individual (bio)molecules, particles, beads and/or the determination of length/size (e.g. hydrodynamic radius). | 10-31-2013 |
20130288233 | Selective Reaction Monitoring (SRM) Derived Protein Profiles for Cancer and other Pathologic Entities - The invention relates to a method of detecting and quantifying small peptides derived from proteins from a range of different clinical samples using the Selective Reaction Monitoring (SRM) profiling technique. By targeting these unique peptides which specifically identify particular proteins, the present invention enables multiple samples to be run in a multiplexed fashion in order to identify, diagnose, quantitate and profile a full range of benign and pathologic entities, including but not limited to, the complete range of cancers and the spectrum of inflammatory diseases, including inflammatory cell typing and bone marrow cell typing. The SRM assay is capable of performing clinical blood typing and it can also act as a diagnostic test to identify women at highest risk for cervical cancer base on Human Papillomavirus (HPV) testing. | 10-31-2013 |
20130295553 | COMPOSITIONS AND METHODS RELATING TO MOUSE PAPILLOMA VIRUS - Methods and compositions for detecting MusPV, also known as MmuPv1, infection of a rodent subject are described according to aspects of the present invention. In specific aspects, the present invention relates to assays for detecting MusPV infection of a rodent subject; vaccine compositions for inducing an immunological response against MusPV in a rodent subject; methods of inducing an immunological response to MusPV in a rodent subject; isolated MusPV protein; isolated antibodies which specifically binds to an MusPV protein, a fragment or variant thereof; isolated recombinantly expressed MusPV proteins; expression constructs comprising a nucleic acid encoding an MusPV protein; host cells comprising the expression construct; and hybridoma cell lines expressing an anti-MusPV monoclonal antibody specific for MusPV. | 11-07-2013 |
20130295554 | METHODS AND COMPOSITIONS FOR THE DETECTION OF TARGET MOLECULES - The present disclosure provides methods and compositions for the detection of target molecules, comprising modified bacteriophages engineered to express a luciferase. | 11-07-2013 |
20130295555 | BIO-MOLECULE DETECTING DEVICE AND BIO-MOLECULE DETECTING METHOD - A bio-molecule detecting device that enables high-sensitivity measurement is provided. The bio-molecule detecting device was configured so that the orientation directions of free molecules and binding molecules in a solution are switched by switching the radiation direction of orientation control light, whereby the light extinction amount can be changed using nanorods associated with the free molecules and the binding molecules. In addition, between the free molecules and the binding molecules, there is a difference in the necessary time for the orientation direction to be switched by switching the radiation direction of the orientation control light, and therefore the timings, at which the light extinction amounts by the respective molecules change, are different. | 11-07-2013 |
20130295556 | Bacteria Identification by Phage Induced Impedance Fluctuation Analysis - Methods for detection and identification of bacteria within a sample include the step of inserting a pair of electrodes into the sample. A first impedance across the electrodes is established with a first AC voltage source having a first frequency. A phage is introduced into the sample, and impedance fluctuations that are caused by ion release by the bacteria due to the phage introduction are measured. The use of impedance fluctuations instead of voltage fluctuations to detect and identify bacteria minimizes 1/f noise effects and increases system sensitivity. To further increase system sensitivity by eliminating thermal noise, a second impedance across the electrodes can be established using a second AC voltage source at a second frequency. Second impedance fluctuations are cross-correlated to the first impedance fluctuations, and the cross-correlation results are analyzed to determine whether or not bacteria are present in the sample based on phage electrical activity. | 11-07-2013 |
20130295557 | SELECTIVE DETECTION OF HAEMOPHILUS INFLUENZAE - A process for detecting | 11-07-2013 |
20130302782 | Methods for the Phenotypic Detection of HCV Inhibitor Resistant Subpopulations - Methods and compositions for the efficient and accurate determination of susceptibility of a hepatitis C virus (HCV) population to an HCV inhibitor are provided. In certain aspects, the methods involve introducing into a cell a patient derived segment, wherein the cell or the patient derived segment comprises an indicator nucleic acid that produces a detectable signal that is dependent on the HCV; measuring the expression of the indicator gene in the presence of varying concentrations of the HCV inhibitor; determining a standard curve of susceptibility; comparing the IC | 11-14-2013 |
20130302783 | PRETREATMENT METHOD OF BIOLOGICAL SAMPLE, DETECTION METHOD OF RNA, AND PRETREATMENT KIT - The present invention is to provide a pretreatment method that allows RNA to be detected promptly and simply. RNA degradation activity due to lactoferrin present in the human rhinal mucosa is inhibited, for example, by adding iron ion and carbonate ion to a biological sample that contains the human rhinal mucosa. With the pretreated biological sample, an RNA virus gene can be amplified by a reverse transcriptase. Iron ion and carbonate ion can also inhibit reverse transcriptase inhibition due to lysozyme C contained in the human rhinal mucosa. Further, it is preferable to remove the envelope of the RNA virus by adding SDS to the biological sample that contains the human rhinal mucosa. | 11-14-2013 |
20130302784 | OPTIMIZED PROBES AND PRIMERS AND METHODS OF USING SAME FOR THE BINDING, DETECTION, DIFFERENTIATION, ISOLATION AND SEQUENCING OF HERPES SIMPLEX VIRUS - Described herein are primers and probes useful for the binding, detecting, differentiating, isolating, and sequencing of HSV-1 and/or HSV-2 viruses. | 11-14-2013 |
20130302785 | METHODS AND DEVICES FOR ELECTRONIC SENSING - The presence of a detectable entity within a detection volume of a microfabricated elastomeric structure is sensed through a change in the electrical or magnetic environment of the detection volume. In embodiments utilizing electronic detection, an electric field is applied to the detection volume and a change in impedance, current, or combined impedance and current due to the presence of the detectable entity is measured. In embodiments utilizing magnetic detection, the magnetic properties of a magnetized detected entity alter the magnetic field of the detection volume. This changed magnetic field induces a current which can reveal the detectable entity. The change in resistance of a magnetoresistive element may also reveal the passage of a magnetized detectable entity. | 11-14-2013 |
20130302786 | Positive Control Concept - Methods and kits are provided for detecting or quantitating at least two different target nucleic acids using a single positive control stock solution comprising a mixture of positive control nucleic acids for the different target nucleic acids to be detected or quantitated. | 11-14-2013 |
20130302787 | CARTRIDGE FOR USE IN AN AUTOMATED SYSTEM FOR ISOLATING AN ANALYTE FROM A SAMPLE, AND METHODS OF USE - A device for extraction or isolation of an analyte, such as a nucleic acid, a protein, or a cell, from a sample, and in particular from a biological sample, is described. Methods of using the device are also described. Further processes, such as amplification of the isolated analyte, may also be carried out within the device. | 11-14-2013 |
20130302788 | RAPID DETECTION OF ANALYTES IN LIQUID SAMPLES - A device for detecting at least one analyte (and in some embodiments two, three, or four or more different analytes) in a liquid sample generally comprises (i) a support having a chamber for receiving a biological fluid therein, wherein said chamber is an elongate chamber having a length axis; (ii) a carrier or agitator in said elongate chamber, said carrier or agitator having opposite end portions and a side portion, the carrier or agitator dimensioned to travel in said chamber along said length axis and/or permit said liquid sample to flow in the chamber therearound, either (or both) thereby agitating the liquid sample; and (iii) at least one anti-analyte antibody coupled to either the carrier and/or the chamber side wall. | 11-14-2013 |
20130302790 | INFECTIOUS HEPATITIS E VIRUS GENOTYPE 3 RECOMBINANTS - The invention relates to the discovery of an HEV strain from a chronically infected patient. The virus grow unusually well in numerous cell cultures. Thus, the invention provides cell cultures, vectors, and vaccine compositions based on the virus. The invention relates, in part, on the identification of a new strain of HEV genotype 3 virus. Strain Kernow-C1 (genotype 3) of HEV, which was isolated from a chronically infected patient, was used to identify human, pig and deer cell lines permissive for infection. Adaptation of the Kernow-C 1 strain to growth in human hepatoma cells selected for a rare virus recombinant that contained an insertion of 174 ribonucleotides (58 amino acids) of a human ribosomal protein gene and additional mutations. | 11-14-2013 |
20130309656 | Antibody detection method and device for a saliva sample from a non-human animal - A rapid test apparatus, system, and method of use utilizing lateral flow immunoassay (LFIA) detection of a selected ligand in a liquid sample from a body fluid such as saliva in a pet in which antibodies and their complimentary antigens are used with detection-nanoparticles to provide a visual or measurable end point indicator in which the method measures the exposure to viruses in the canine from Canine Parvovirus (CPV) and/or Canine Distemper virus (CDV). | 11-21-2013 |
20130309657 | DEVICES AND METHODS FOR SEPARATING PARTICLES - Embodiments of the present disclosure provide for particle-imprinted polymer films, methods of making particle-imprinted polymer films, methods for separating particles, devices or systems for separating particles, and the like. | 11-21-2013 |
20130309658 | METHOD FOR DETECTING HUMAN PAPILLOMAVIRUS mRNA - An in vitro method is provided for screening human female subjects to assess their risk of developing cervical carcinoma which comprises screening the subject for expression of mRNA transcripts from the E6 and optionally the L1 gene of human papillomavirus, wherein subjects positive for expression of L1 and/or E6 mRNA are scored as being at risk of developing cervical carcinoma. Kits for carrying out such methods are also provided. | 11-21-2013 |
20130316326 | ANIMAL MODEL FOR THE EVALUATION OF THE EFFICACY OF AN HIV VACCINE - The present invention relates to the use of a Severe Combined T-B-Immune Deficient (SCID) mouse engrafted with human immunocompetent cells (Hu-SCID-mouse) as an animal model for the evaluation of the effectiveness of an HIV vaccine. Furthermore, the present invention relates to a method for the evaluation of an HIV vaccine, wherein a Hu-SCID-mouse of the invention is inoculated with the HIV vaccine and thereafter challenged with HI-virus. The invention also relates to novel HIV vaccine compositions, which can be evaluated using the animal model. | 11-28-2013 |
20130316327 | NMR DIAGNOSTICS BY MEANS OF A PLASTIC SAMPLE CONTAINER - Sample containers and methods for employing the same in in-vitro nuclear magnetic resonance measurements are provided. The sample containers are made of a material that comprises on or more polymeric materials. | 11-28-2013 |
20130316328 | COMPOSITIONS AND METHODS FOR DETECTING CERTAIN FLAVIVIRUSES, INCLUDING MEMBERS OF THE JAPANESE ENCEPHALITIS VIRUS SEROGROUP - The present invention provides rapid and accurate methods, primers, probes and kits for identifying the presence of a certain flaviviruses in a sample. Flaviviruses that can be detected include members of the Japanese encephalitis virus serogroup, Dengue virus, St. Louis encephalitis virus, Montana myotis leukoencephalitis virus, Modoc virus, and Yellow Fever virus. The primers and probes of the invention can hybridize to regions in the 3′ untranslated region of the viral genomes to be detected. | 11-28-2013 |
20130316329 | MICRO-DEVICES FOR DISEASE DETECTION - Among others, the present invention provides piezo-electric micro-devices for detecting at the microscopic level an electric, magnetic, electromagnetic, thermal, optical, acoustical, biological, chemical, physical, bio-chemical, bio-physical, physical-chemical, bio-physical-chemical, bio-mechanical, bio-electro-mechanical, electro-mechanical, or mechanical property of the biologic subject. | 11-28-2013 |
20130316330 | NON-TOXIC SOLVENT FOR CHROMOGENIC SUBSTRATE SOLUTION AND USES THEREOF - The present invention relates to a non-toxic dipolar solvent for chromogenic substrate for detecting presence of lacZ gene and/or gene activity, which comprises a stabilizing amount of a solubilizing agent. The present invention also relates to a method for inducing lac operon in screening assay, comprising the step of contacting an agar plate with at least one essential oil in a concentration sufficient to induce the lac operon. The present invention further relates to a method for detecting the presence of bacteria, comprising the step of contacting an agar plate with at least one essential oil in a concentration sufficient to induce detection of the bacteria. | 11-28-2013 |
20130316331 | DETECTION OF INFECTION BY A MICROORGANISM USING SMALL RNA SEQUENCING SUBTRACTION AND ASSEMBLY - There is provided a method for the detection and identification of infection of a subject by a microorganism, wherein the method is based on the use of small RNA derived sequences and subtraction and assembly thereof. | 11-28-2013 |
20130316332 | COMBINATION OF BIOMARKERS FOR THE PROGNOSIS OF RESPONSE OR NON-RESPONSE TO AN ANTI-HCV TREATMENT - The application concerns means for predicting whether a subject infected with one or more HCVs has a high probability of responding to an anti-HCV treatment which will comprise the administration of interferon and of ribavirin or whether, in contrast, that subject has a high probability of not responding to that anti-HCV treatment. The means of the invention in particular involve assaying the levels of expression of selected genes, said selected genes being:
| 11-28-2013 |
20130316333 | Method for Detecting and Quantifying Microorganisms - The present invention relates to a method for detecting at least one microorganism in a sample, including: cultivating the sample in a liquid medium in the presence of at least one specific ligand of the microorganism, and at least one scavenger having a lower affinity to the microorganism than the ligand, the binding of a compound to the ligand producing a first measurable signal and the binding of a compound to the scavenger producing a second measurable signal; determining the values of the first and second signals for at least one cultivation period; wherein it is deduced that the sample includes the microorganism when the values of the first signal and second signal are different for the same cultivation period. | 11-28-2013 |
20130316334 | TRANSFORMATION OF MATERIAL INTO AN OPTICALLY MODULATING STATE VIA LASER RADIATION - A method for the transformation of material (e.g. plastic material) into an optically modulating state via laser radiation is described. The optically modulating state may be a state in which light is emitted at a different wavelength than it is absorbed. The plastic material to may be a thermoplastic or elastomeric material, or an organic polymer selected from the group consisting of polyethylene, polypropylene, polystyrene, polycarbonate and polycycloolefin. The laser radiation may comprise the application of an amount of energy of about 0.1 nJoule/μm | 11-28-2013 |
20130323716 | SIGNAL ENCODING AND DECODING IN MULTIPLEXED BIOCHEMICAL ASSAYS - This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte. | 12-05-2013 |
20130323717 | REPEAT-CHAIN FOR THE PRODUCTION OF DIMER, MULTIMER, MULTIMER COMPLEX AND SUPER-COMPLEX - The present invention relates to a method for manufacturing multimers by making repeat-chains comprising repeatedly linked affinity domains binding specifically to monomers, and by using the same to create a repeat-chain/multiple-monomer complex created from the repeat-chains and a multiple number of monomers, thereby facilitating the formation of bond bridges between the monomers in the complex to produce inter-monomeric bond bridged multimer. | 12-05-2013 |
20130323718 | Methods for the Characterization of Microorganisms on Solid or Semi-Solid Media - The present invention relates to methods and systems for scanning, detecting, and monitoring microorganisms on solid or semi-solid media using intrinsic fluorescence (IF) measurements. The methods are further directed to detection, characterization and/or identification of microorganisms on a solid or semi-solid media using intrinsic fluorescence (IF) measurements that are characteristic of said microorganisms. | 12-05-2013 |
20130323719 | NEW IRIDIUM-BASED COMPLEXES FOR ECL - Novel iridium-based Ir(III) luminescent complexes, conjugates comprising these complexes as a label and their application, for example in the electrochemiluminescence based detection of an analyte. | 12-05-2013 |
20130323720 | COMBINATION OF BIOMARKERS FOR THE DETECTION AND EVALUATION OF HEPATITIS FIBROSIS - The application concerns means for determining the stage of hepatic tissue damage, in particular the hepatic fibrosis score of subjects infected with one or more hepatitis viruses. In particular, the means of the invention involve measuring the levels of expression of selected genes, said selected genes being:
| 12-05-2013 |
20130330710 | SILK BASED BIOPHOTONIC SENSORS - The present disclosure relates to biophotonic sensors. An example of a biophotonic sensor may be an apparatus for analyzing a sample. The apparatus may include a substrate, aperiodic nanostructured protrusions disposed on the substrate, and a silk material deposited between the protrusions. | 12-12-2013 |
20130330711 | SENSOR FOR DETECTION OF A TARGET OF INTEREST - Embodiments of the present disclosure set forth an apparatus of a sensor for detecting a target of interest. One example apparatus may comprise a substrate, a material disposed on the substrate and a probe disposed on the material. The probe is configured to bind to the target of interest and scatter light emitted from a light source when the target of interest is bound to the probe. | 12-12-2013 |
20130330712 | SENSOR FOR DETECTION OF A TARGET OF INTEREST - Embodiments of the present disclosure set forth a sensor for detecting a target of interest. One exemplary sensor may comprise a container; a probe disposed in the container; a circulation means configured to circulate substances in the container; a light source; a light receiver; and a detector configured to generate an electrical signal, the magnitude of which reflects the amount of light that is received by the light receiver, wherein the sensor is located between the light source and the light receiver, and wherein the sensor is configured such that the probe and the path of the light emitted by the light source are in different regions inside the container. | 12-12-2013 |
20130330713 | LATERAL FLOW ASSAY DEVICES FOR USE IN CLINICAL DIAGNOSTIC APPARATUS AND CONFIGURATION OF CLINICAL DIAGNOSTIC APPARATUS FOR SAME - A lateral flow device for use in a mainframe or point-of-care clinical analyzer, in which the lateral flow device includes a planar support having at least one sample addition area and at least one reaction area disposed thereon. The sample addition area and reaction area are fluidly interconnected to one another and form at least one lateral fluid flow path. The lateral flow device is sized for retention within a storage cartridge of the analyzer defined by a hollow interior and having a plurality of lateral flow assay devices retained in stacked relation therein. | 12-12-2013 |
20130330714 | MASS SPECTROMETRY ANALYSIS OF MICROORGANISMS IN SAMPLES - The invention generally relates to systems and methods for mass spectrometry analysis of microorganisms in samples. | 12-12-2013 |
20130330715 | ASSAYS AND METHODS TO SEQUENCE MICROBES DIRECTLY FROM IMMUNE COMPLEXES - Provided herein are MiIP-Seq assays and methods for detecting and/or identifying an immune-stimulating microbe in a patient sample. Also provided herein are methods for diagnosing an infectious disease or identifying a previously uncharacterized microbe in a patient sample. The methods and assays described herein are advantageous over existing methods in that they (i) do not require a culture step for microbe expansion, (ii) are not specific for a particular microbe and can be used to identify a previously uncharacterized microbe, and (iii) permits rapid processing due to the lack of a microbe culture step. | 12-12-2013 |
20130330716 | METHOD FOR DETERMINING THE TITRE OF VIRUSES BY USING INFECTIOUS STANDARDS - The present invention is directed to a titration method for cytopathogenic agent, particularly infectious viruses. In particular, the present invention is based on a new method for in vitro determining the titre of infectious agent, such as viruses in solution by comparing the viability of the infected cells (in monolayer conformation or in suspension) against a standard curve obtained by measuring the cell viability of infected host cells cultured in monolayer or in suspension with a known cytopathogenic agent stock. The invention is of special utility in the pharmaceutical industry for cytopathogenic agent clearance studies and for the identification and optimization of antiviral compounds involved in drug development and/or in pharmaceutical compositions. | 12-12-2013 |
20130337434 | Method of Preparing Magnetic Bead Type Nasopharyngeal Enzyme Immunoassay Reagents by Polymerase Chain Reaction - A method of preparing magnetic bead type nasopharyngeal enzyme immunoassay reagents by polymerase chain reaction according to this invention offers in-vitro diagnostic reagents by means of utilizing nanotechnology. This method uses magnetic beads to coat EBV nuclear antigen (Epstein-Barr virus nuclear antigen, EBNA1) or early antigen (Early Antigen, EA). The use of polymerase chain reaction (polymerase Chain Reaction PCR) is for amplified detection. It is found that positive controls of different concentrations, after amplified by PCR, change in their brightness and concentration. That reveals the EBV antigens on the magnetic beads can specifically detect IgA antigens in the serum. | 12-19-2013 |
20130337435 | M13 Bacteriophage as a Chemoaddressable Nanoparticle for Biological and Medical Applications - Reactive and modified M13 bacteriophages, and methods of making and using the same, are generally provided. The reactive M13 bacteriophage can include a alkyne functional group covalently attached to the M13 bacteriophage. The modified M13 bacteriophage can include a substituent covalently attached to the M13 bacteriophage via a 1,2,3-triazole linkage. Dual-modified M13 bacteriophages are also generally provided, and can include a cancer-targeting substituent covalently attached to the M13 bacteriophage and a fluorescent group covalently attached to the M13 bacteriophage. The modified M13 bacteriophages can not only be employed as a fluorescent probe for cancer imaging, but also can be used as biomaterials for cell alignment and scaffolding. | 12-19-2013 |
20130337436 | SUBSTRATE COATED WITH NANOPARTICLES, AND USE THEREOF FOR THE DETECTION OF ISOLATED MOLECULES - The present disclosure relates to a substrate having a surface comprising nanoparticles or groups of nanoparticles which the linear optical response does not depend on the polarisation of the incident field of a Gaussian beam having an axis of propagation that is directed perpendicularly to the surface of the substrate. The shape or arrangement of the groups of nanoparticles has a Cn-type axis of symmetry perpendicular to the surface, where n is a number equal to three or greater than four, and the shape of the nanoparticles has a Cn-type axis of symmetry perpendicular to the surface, where n is a number no less than three, to allow strong enhancement of the beam close to the surface. The disclosure relates to use of substrates for detection and/or measurement of chemical, biochemical or biological molecules, wherein the molecules can be present in trace amounts in a liquid or other medium. | 12-19-2013 |
20130337437 | PROCESS FOR DETECTING CELLS FROM A SAMPLE - A process for detecting cells from a sample, comprising the following steps:
| 12-19-2013 |
20130337438 | MONOCLONAL ANTIBODY RECOGNIZING HUMAN PAPILLOMAVIRUS (HPV) L2 PROTEIN AND METHOD FOR MEASURING HPV-NEUTRALIZING ANTIBODY TITER USING THE SAME - The present invention relates to the development of a monoclonal antibody which has binding activity to many high-risk types of HPV, etc. The present invention also provides a simple and high-throughput method for measuring cross-neutralizing antibody titers, which is used for assay of cross-neutralizing antibody against HPV in serum samples from subjects, etc. The method of the present invention for measuring cross-neutralizing antibody titers comprises the steps of: preparing a monoclonal antibody against a peptide having a specific amino acid sequence common to high-risk types of HPV; and assaying cross-neutralizing antibody using this monoclonal antibody. | 12-19-2013 |
20130337439 | PORTABLE DEVICE FOR THE STORAGE, TRANSPORT AND RECUPERATION OF BIOLOGICAL MATERIAL - The present invention refers to a portable device for the collection of biological samples. The device comprises a receptacle ( | 12-19-2013 |
20130344474 | METHOD AND KIT FOR DETECTION OF CHRONIC HEPATITIS B - The novel means by which whether a subject has chronic hepatitis B or not can be determined easily with a high degree of accuracy is disclosed. The method for detection of chronic hepatitis B according to the present invention comprises measuring the amount of anti-HBc IgG in a sample separated from a subject. In this method, the measured amount of anti-HBc IgG which is not less than a predetermined cut-off value is indicative of chronic hepatitis B in the subject. The cut-off value may be a value not less than 40 IU/mL, e.g., a value selected from the range of 100 IU/mL to 200 IU/mL. By the present invention, chronic hepatitis B can be detected based on the amount of anti-HBc IgG without measuring the total amount of HBc antibodies including anti-HBc IgM. | 12-26-2013 |
20130344475 | Microfluidic Devices - Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense. | 12-26-2013 |
20130344476 | HEPATITIS C VIRUS VARIANTS - The present invention relates to HCV variants, particularly variants that are resistant to a protease inhibitors such as VX-950. Also provided are methods and compositions related to the HCV variants. Further provided are methods of isolating, identifying, and characterizing multiple viral variants from a patient. | 12-26-2013 |
20130344477 | METHODS OF CAPTURING BINDABLE TARGETS FROM LIQUIDS - A bindable target such as a bacterial cell, virus, or molecule is captured from a liquid by contacting the liquid with magnetically attractable particles have an affinity for the target, and causing said particles to move repeatedly through said liquid to at least one solid support zone by attractive magnetic forces to capture the target onto said particles. The particles may be ferromagnetic, paramagnetic or superparamagnetic particles and may bear antibody, antibody binding fragments, a substance having an epitope capable of reacting in a specific manner with an antibody, an aptamer, a nucleic acid sequence or a nucleic acid analogue sequence, biotin, avidin or streptavidin. The particles may be moved back and forth in the liquid between separated solid support zones by attractive magnetic forces which attract the particles temporarily to different solid support zones in turn | 12-26-2013 |
20130344478 | APPARATUS AND METHOD FOR PROCESSING A SAMPLE - A first apparatus ( | 12-26-2013 |
20130344479 | METHOD FOR EARLY DIAGNOSIS OF CARCINOMAS OF THE ANOGENITAL TRACT - A method for the early diagnosis of carcinomas of the anogenital tract, preferably of the cervical carcinoma, and the preliminary stages thereof is described. The method is based on the determination of the methylation status of segments of the gene regions of ASTN1 (astrotactin 1) and ZNF671 (zinc finger protein 671, transcription factor). A DNA methylation in the promoter region or the 5′-region of these genes is indicative for carcinomas of the anogenital tract or severe preliminary stages thereof. The present invention also relates to kits which permit the conduction of the diagnostic method according to the invention. | 12-26-2013 |
20140004502 | SEQUENCES AND METHODS FOR DETECTING INFLUENZA A AND INFLUENZA B VIRUS | 01-02-2014 |
20140004503 | Device and Method for Sampling Bodily Fluid for Medical Analytes in Ultra Low Concentrations | 01-02-2014 |
20140004504 | COMPOSITIONS AND METHODS FOR THE DETECTION AND ANALYSIS OF AFRICAN SWINE FEVER VIRUS | 01-02-2014 |
20140004505 | CARTRIDGE BASED SYSTEM AND METHOD FOR DETECTING AN ANALYTE IN A SAMPLE | 01-02-2014 |
20140004506 | Membrane-Based Lateral Flow Assay Devices that Utilize Phosphorescent Detection | 01-02-2014 |
20140011190 | METHOD FOR PERFORMING A RAPID TEST - The present invention relates to a method for performing a quantitative or qualitative analysis by a two-phase rapid test. In the method a signal reagent is placed into a container made of non-absorbing material, the container being located separate from the rapid test device. The signal reagent is reacted with the analyte(s) present in a test sample by adding the liquid test sample into the container. A reaction solution is obtained, which is then contacted with the rapid test device, wherein a detectable signal is developed. Subsequently, the development of the signal is monitored and read continuously. | 01-09-2014 |
20140011191 | DEVICE AND METHODS FOR QUANTIFYING ANALYTES - The present invention relates to devices and methods for measuring the quantity of multiple analytes in a sample. The device is designed such that each of the analyte sensing elements is configured to measure the quantity of a predetermined analyte and where the machine executable instructions are configured to select the proper analyte sensing element corresponding to the analyte to be measured. | 01-09-2014 |
20140017668 | COMPOSITIONS AND METHODS FOR DETECTING AND QUANTIFYING CIRCULATING TUMOR CELLS (CTCs) - The present invention relates to the field of virology. More specifically, the present invention relates to the use of viral constructs to detect and quantify circulating tumor cells. In one embodiment, the present invention provides an adenovirus construct comprising (a) a cell type specific promoter that drives adenoviral replication; and (b) at least one reporter gene incorporated into the viral Major Late Transcriptional Unit. In another embodiment, an adenovirus construct comprises (a) prostate selective pro-basin promoter operably linked to the El gene; and (b) prostate specific antigen enhancer operably linked to the probasin promoter. | 01-16-2014 |
20140017669 | Signal Amplification - The present invention relates to compositions and methods for the use of enzymes composed of nucleic acid and/or protein enzymes to generate and amplify a signal indicative of the presence of a target. More particularly, the invention relates to compositions comprising nucleic acid structures that serve as partial or complete enzyme substrates and methods for using these structures to facilitate detection of targets. | 01-16-2014 |
20140017670 | MICRO-DEVICES FOR DISEASE DETECTION - Among others, the present invention provides micro-devices for detecting or treating a disease, each comprising a first micro sensor for detecting a property of the biological sample at the microscopic level, and an interior wall defining a channel, wherein the micro sensor is located in the interior wall of the micro-device and detects the property of the biological sample in the microscopic level, and the biological sample is transported within the channel. | 01-16-2014 |
20140017671 | DETECTION METHOD OF NUCLEIC ACID AND KIT AND USING THEREOF - A detection method of nucleic acid is provided. The method includes: providing nucleic acid to be tested, making the nucleic acid to be tested react under asymmetric PCR conditions with a pair of primers for target nucleic acid amplification, DNA polymerase, adenine deoxyribonucleotide, guanine deoxyribonucleotide, cytosine deoxyribonucleotide and thymidine deoxyribonucleotide in a PCR buffer solution, mixing the reaction product and liquid that contains probe molecules, and judging whether the nucleic acid to be tested contains the target nucleic acid by observing the obtained mixture color or color change. A kit is also provided that can be used in the nucleic acid detection by the said method. Application of the method and the kit in inspection and quarantine is also provided. The method is a quick and easy, sensitive and a specific detection method of nucleic acid with direct observation using naked eyes. The method does not need additional equipment. | 01-16-2014 |
20140017672 | METHOD AND KIT FOR PURIFYING NUCLEIC ACIDS - Methods for automated extraction of nucleic acids are disclosed. Also disclosed are method and kits for isolating fetal nucleic acids from a plasma sample of a pregnant woman. The method includes flowing the plasma sample through a first filter under conditions that allow binding of the fetal and maternal nucleic acids to the first filter; eluting the fetal and maternal nucleic acids bound to the first filter to produce a concentrated nucleic acid sample; flowing the concentrated nucleic acid sample through a second filter under conditions that allow preferential binding of the maternal nucleic acids to the second filter; and recovering the fetal nucleic acid from the concentrated nucleic acid sample that flow through the second filter. | 01-16-2014 |
20140024013 | Isolation, Cloning and Characterization of New Adeno-Associated Virus (AAV) Serotypes - The present invention provides new adeno-associated virus (AAV) viruses and vectors, and particles derived therefrom. In addition, the present invention provides methods of delivering a nucleic acid to a cell using the AAV vectors and particles. | 01-23-2014 |
20140024014 | PRIMER SET, METHOD AND KIT FOR DETECTING PATHOGEN IN ANIMALS OR PLANTS - The invention provides a method for rapidly detecting a pathogen in animals or plants comprising using a specific primer set and nucleic acid in a sample to carry on a loop-mediated isothermal amplification. If at least one amplification occurs, the sample comprises the pathogen. The invention also provides a primer set, probe and kit for detecting a pathogen in animals or plants. | 01-23-2014 |
20140024015 | Boone Cardiovirus - The invention provides an isolated Boone cardiovirus, Boone cardiovirus polypeptides, polynucleotides and antibodies specific for Boone cardiovirus polypeptides. Also provided are methods for detection of Boone cardiovirus. | 01-23-2014 |
20140024016 | LATERAL FLOW ASSAYS USING TWO DIMENSIONAL FEATURES - The present invention relates to novel lateral flow devices using two dimensional features, preferably, uniform two dimensional test and control features, to provide flow control information, to function as an internal control and/or to provide internal calibration information for the test device. The present invention also relates to methods for using the lateral flow devices to provide flow control information, to function as an internal control and/or to provide internal calibration information for the test device. | 01-23-2014 |
20140024017 | IDENTIFICATION OF A NOVEL HUMAN POLYOMAVIRUS (IPPyV) AND APPLICATIONS - The invention relates to the identification of a novel human polyomavirus species (designated IPPyV) and applications derived from the identified features and properties of this virus. The IPPy virus species of the invention qualifies as a human virus, in view of the fact that it is capable of infecting a human host. Having identified a novel human polyomavirus species, IPPyV, the inventors have been able to propose means for the detection of exposure or infection by such a virus, especially detection in a biological sample previously obtained from a human host. The invention also concerns means suitable for obtaining an immune response in a host with a view to prevent the onset or the development of an infection with an IPPyV. | 01-23-2014 |
20140024018 | METHOD FOR THE PROGNOSIS OF PROGRESSION OF THE HIV DISEASE - An in vitro method for the prognosis of progression of an HIV-1 infection in a patient infected with an HIV-1 virus, comprising the steps of: a) measuring the level of antibodies directed against the 3S peptide of SEQ ID NO 2 in a sample collected from the said patient, b) comparing the level of anti-3S antibodies measured at step a) with a reference value of anti-3S antibody level that is indicative of the progression of the HIV-1 infection. | 01-23-2014 |
20140024019 | Methods and Means for Monitoring Disruption of Tissue Homeostasis in the Total Body - The invention relates to the field of medical diagnostics. Provided are methods and kits for determining the health status of a subject, for early detection of tissue damage, for early diagnosis and monitoring of a disease, and/or for evaluation of treatment effectiveness in a subject using circulating tissue macrophages (CTM) as a mirror of disrupted tissue homeostasis and disease. | 01-23-2014 |
20140024020 | OPTICAL ANALYSIS DEVICE, OPTICAL ANALYSIS METHOD AND COMPUTER PROGRAM FOR OPTICAL ANALYSIS USING SINGLE LIGHT-EMITTING PARTICLE DETECTION - There is provided a structure to reduce the size of light intensity data in the scanning molecule counting method using an optical measurement with a confocal microscope or a multiphoton microscope. In the inventive optical analysis technique of detecting light of a light-emitting particle in a sample solution, time series light intensity data of light from a light detection region detected with moving the position of the light detection region of the microscope in the sample solution is generated, and a signal of a light-emitting particle individually is detected in the time series light intensity data. In that case, regions where no signal indicating light of light-emitting particles exist in the time series light intensity data is removed from the time series light intensity data. | 01-23-2014 |
20140024021 | COMPOSITIONS, REACTION MIXTURES AND METHODS FOR DETECTING NUCLEIC ACIDS FROM MULTIPLE TYPES OF HUMAN PAPILLOMAVIRUS. - Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed. | 01-23-2014 |
20140030697 | SORTASE-MEDIATED MODIFICATION OF VIRAL SURFACE PROTEINS - The present invention, in some aspects, provides methods, reagents, and kits for the functionalization of proteins on the surface of viral particles, for example, of bacteriophages, using sortase-mediated transpeptidation reactions. Some aspects of this invention provide methods for the conjugation of an agent, for example, a detectable label, a binding agent, a click-chemistry handle, or a small molecule to a surface protein of a viral particle. Kits comprising reagents useful for the generation of functionalized viral particles are also provided, as are precursor proteins that comprise a sortase recognition motif, and viral particles comprising such precursor proteins. Nucleic acids encoding viral proteins comprising a sortase recognition motif and expression vectors comprising such nucleic acids are also provided. | 01-30-2014 |
20140030698 | MULTIPLEX ASSAY FOR THE DETECTION OF CITRUS PATHOGENS - The present invention provides methods and compositions for detecting multiple citrus pathogens using a multiplex branched DNA signal amplification reaction. | 01-30-2014 |
20140030699 | ELECTRICALLY CONDUCTIVE POLYMER ELECTRODES WITH INCORPORATED VIRUSES - Grafting M13 bacteriophage into an array of poly(3,4-ethylenedioxythiophene) (PEDOT) nanowires generated hybrids of conducting polymers and replicable genetic packages (rgps) such as viruses. The incorporation of rgps into the polymeric backbone of PEDOT occurs during electropolymerization via lithographically patterned nanowire electrodeposition (LPNE). The resultant arrays of rgps-PEDOT nanowires enable real-time, reagent-free electrochemical biosensing of analytes in physiologically relevant buffers. | 01-30-2014 |
20140030700 | ASSAYS FOR PATHOGEN DETECTION USING MICROWAVES FOR LYSING AND ACCELERATING METAL-ENHANCED FLUORESCENCE - The present invention relates to an assay for the determination of a microorganism including a lysing chamber having triangular shaped metallic structures wherein the apexes of two triangles are arranged in alignment and forming a reactive zone for placement of the microorganism and lysing by microwave energy for exposing and isolating a target polynucleotide sequence. The isolated target polynucleotide sequence is introduced to an assay system for contact with polynucleotides which are complimentary to the isolated target polynucleotide sequence. Fluorophore-labeled capture polynucleotides are added for hybridizing to any bound target polynucleotide. Bound target polynucleotides are detected by metal enhanced fluorescence. | 01-30-2014 |
20140030701 | COMPOSITIONS, REACTION MIXTURES AND METHODS FOR DETECTING NUCLEIC ACIDS FROM TYPE A1 AND/OR TYPE C1 HUMAN PAPILLOMAVIRUS - Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed. | 01-30-2014 |
20140030702 | MODIFIED INFLUENZA VIRUS FOR MONITORING AND IMPROVING VACCINE EFFICIENCY - The immunogenicity of the influenza virus hemagglutinin (HA) molecule may be increased by substitutions of amino acids in the HA sequence. The substitution of specific HA residues, such as asparagine at position 223 of H5 HA, increase the sensitivity of the hemagglutinin inhibition (HI) assay by altering receptor specificity and/or antibody-antigen binding. HA molecules containing such substitutions will be useful in the development of diagnostic reference viruses and improved influenza vaccines. | 01-30-2014 |
20140030703 | Compositions and Methods for Detecting and Identifying Nucleic Acid Sequences in Biological Samples - The invention is directed to compositions and methods for isolating, detecting, amplifying, and quantitating pathogen-specific nucleic acids in a biological sample. The invention also provides diagnostic kits containing specific amplification primers, and labeled detection probes that specifically bind to the amplification products obtained therefrom. Also disclosed are compositions and methods for the isolation and characterization of nucleic acids that are specific to one or more pathogens, including for example Influenza virus and | 01-30-2014 |
20140038167 | FLUID DELIVERY SYSTEM AND METHOD - A method and apparatus for delivering one or more fluids. Fluids may be delivered sequentially from a common vessel to a chemical, biological or biochemical process. | 02-06-2014 |
20140038168 | NOVEL DRUG TARGET SITE WITHIN GP120 OF HIV - The present invention relates to a method of designing an inhibitor of the binding of HIV (human immunodeficiency virus) glycoprotein (gp)120 to a CD4-receptor or to the integrin alpha4 beta7 (a4b7). The inhibitor interacts with at least two amino acid residues comprised in six motifs within the 3-dimensional structure of gp120. Also provided are compounds, pharmaceutical compositions thereof and uses thereof in the development of an inhibitor of the binding of a HIV gp120 to a CD4-receptor or an integrin alpha4 beta7 (a4b7). The inhibitors are useful for the prevention or treatment of an HIV infection and/or diseases associated with an HIV infection. | 02-06-2014 |
20140038169 | QUANTIFICATION OF SINGLE TARGET MOLECULES IN HISTOLOGICAL SAMPLES - The present invention lies in the field of visualization and quantification of immobilized targets in samples using immunochemical means. The methods of the invention utilize an immunostaining system allowing visualizing single target units in samples as distinct dots. In particular, the invention relates to methods and reagents for visualization and quantification of molecular targets immunostained in histological samples and use of said method and reagents in medical diagnostic. However, the visualization and quantification methods of the invention are applicable to a variety of targets in different samples and allow precise quantifying both relative and absolute amounts thereof. | 02-06-2014 |
20140038170 | APPARATUS, SYSTEMS, AND METHODS FOR PERFORMING THERMAL MELT ANALYSES AND AMPLIFICATIONS - The present disclosure provides apparatus, systems, and methods for conducting rapid, accurate, and consistent heated amplifications and/or thermal melt analyses. | 02-06-2014 |
20140038171 | RAPID CELL PURIFICATION SYSTEMS - Methods and systems for purifying cells and/or viruses are provided. The sample is added to a well disposed in a medium. A potential is applied across the medium to cause the contaminants to enter one or more walls of the well, and retain the cells and/or viruses in the well. The cells and/or viruses can be removed from the well, and optionally adhered or fixed to a surface, or detected. In one embodiment, the cells and/or viruses may be retained in the well by embedding in the medium. The medium including the embedded cells and/or viruses may be excised or otherwise removed and transferred to a glass slide or other solid surface. | 02-06-2014 |
20140038172 | Matrix and System for Preserving Biological Specimens for Qualitative and Quantitative Analysis - The present invention provides a device, system, and methods of use comprising an absorbent hydrophobic polyolefin matrix, and methods of use thereof, for storage, preserving, and recovering liquid suspension of biological specimens containing analytes of interest in a dry state. The dried biological specimens containing analytes of interest absorbed on the polyolefin matrix are reconstituted such as with molecular-grade water and released by compressing the polyolefin matrix. The reconstituted biological analytes are qualified for subsequent analysis, such as for qualitative and quantitative analysis of viral nucleic acids, such a viral load testing, genotyping, and sequencing. Also provided are kits with instructions, and methods of use thereof, for storage, preserving, and recovering biological specimens containing analytes of interest using the compression device of the invention. | 02-06-2014 |
20140038173 | COMPOSITIONS, REACTION MIXTURES AND METHODS FOR DETECTING NUCLEIC ACIDS FROM MULTIPLE TYPES HUMAN PAPILLOMAVIRUS - Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed. | 02-06-2014 |
20140038174 | Compositions and Methods for Detecting and Identifying Nucleic Acid Sequences in Biological Samples - The invention is directed to compositions and methods for isolating, detecting, amplifying, and quantitating pathogen-specific nucleic acids in a biological sample. The invention also provides diagnostic kits containing specific amplification primers, and labeled detection probes that specifically bind to the amplification products obtained therefrom. Also disclosed are compositions and methods for the isolation and characterization of nucleic acids that are specific to one or more pathogens, including for example Influenza virus and | 02-06-2014 |
20140038175 | IN VITRO TEST METHOD FOR IMPLANT MATERIALS - The invention relates to a method for in vitro testing of specimens, such as biomaterials or implants, wherein the method comprises at least immersing at least a part of the test specimen into a liquid media, controlling the liquid media, controlling surrounding environment, providing a predetermined non-destructive force to the specimen, and measuring reactions of the specimen or constituents of the liquid media. Further it relates to an apparatus for in vitro testing. | 02-06-2014 |
20140045168 | MICROTITER PLATE SYSTEM AND METHOD - A system for analyzing, manipulating, and recording data associated with a microtiter plate is provided. The system provides a touch screen element provided proximal to the microtiter plate such that data pertaining to events and conditions observed within one or more wells of the microtiter plate may be easily and accurately recorded. Data display, storage, and manipulation features are also provided. | 02-13-2014 |
20140045169 | GLYCAN IMMOBILIZED METAL NANOPARTICLES AND USE THEREOF FOR EARLY HIV-1 DETECTION - Disclosed are glycan immobilized metal nanoparticles and a method used thereof for detecting HIV-1 in a saliva sample at early stages of viral infection. The method comprises the steps of: (A) providing glycan immobilized metal nanoparticles which can recognize HIV-1; (B) contacting the glycan immobilized metal nanoparticles with the saliva sample and a mixture is obtained; (C) concentrating the mixture; and (D) determining HIV-1 in the concentrated mixture by an appropriate detecting method. | 02-13-2014 |
20140045170 | METHODS FOR DETECTING AND MEASURING AGGREGATION - Methods, compositions, systems, and devices are provided for performing and analyzing agglutination assays. In one aspect, methods for image analysis of agglutination assays are provided. In another aspects, methods for performing agglutination assays are provided. In one aspect, the methods may be used for the detection of various molecules, including viruses or antibodies against a virus. In another aspect, the methods can be used to determine effective immunization of a subject. | 02-13-2014 |
20140045171 | BENZOXAZOLE-BASED FLUORESCENT METAL ION INDICATORS - Disclosed are benzoxazole-based compounds, kits, and methods of producing and using the described compounds in fluorescence-based detection of analytes (e.g., metal ions). Also disclosed are uses of benzoxazole-based compounds as ratiometric metal ion indicators. | 02-13-2014 |
20140045172 | Dual Path Immunoassay Device - Systems include test cells with sorbent material in a T-shape and defining a first flow path for a solution and a second flow path for a sample, and a test line or test site with immobilized antigens or antibodies or other ligand binding molecules located at the junction of the T. A housing houses the sorbent material and defines a first hole adjacent an end of the first flow path for receiving the solution and a second hole adjacent an end of the second flow path for receiving the sample. | 02-13-2014 |
20140045173 | COMPOUNDS HAVING CHROMOPHORE AND PHOTOREACTIVE GROUPS - The invention relates to the field of hydrophobic photoaffinity marking by element of probes that are multifunctional lipid compounds. The compounds include at least one chromophore group and two lipid chains, one of which includes a photoreactive group. The compounds can be used for the study and identification of hydrophobic regions or domains within proteins, particularly within viruses. | 02-13-2014 |
20140045174 | REAL-TIME PCR POINT MUTATION ASSAYS FOR DETECTING HIV-1 RESISTANCE TO ANTIVIRAL DRUGS - Disclosed are compositions including primers and probes, which are capable of interacting with the disclosed nucleic acids, such as the nucleic acids encoding the reverse transcriptase or protease of HIV as disclosed herein. Thus, provided is an oligonucleotide comprising any one of the nucleotide sequences set for in SEQ ID NOS:1-89, and 96-104. Also provided are the oligonucleotides consisting of the nucleotides as set forth in SEQ ID NOS:1-89, and 96-104. Each of the disclosed oligonucleotides is a probe or a primer. Also provided are mixtures of primers and probes and for use in RT-PCR and primary PCR reactions disclosed herein. Provided are methods for the specific detection of several mutations in HIV. Mutations in both the reverse transcriptase and the protease of HIV can be detected using the methods described herein. | 02-13-2014 |
20140051065 | METHODS FOR DETECTING HEPATITIS B VIRUS SURFACE GENE NON-SENSE MUTATIONS - A method for in vitro detection of the presence of a C-terminal truncation mutation of a hepatitis B virus (HBV) surface (S) gene encoding a small S protein in an isolated nucleic acid sample is disclosed. An in vitro diagnostic kit for use in the aforementioned method is also disclosed. | 02-20-2014 |
20140051066 | HPV DETECTION AND QUANTIFICATION BY REAL-TIME MULTIPLEX AMPLIFICATION - The present invention relates to amplification primers and detection probes, which are useful for the detection of human papillomaviruses (HPV), and more particularly of HPV, which can be oncogenic for the mucosal epithelia. The amplification and detection systems provided by the present invention are group-targeted systems, namely A5-, A6- A7-, and A9-targeted systems. The amplification and detection systems of the invention allow for an amplification of HPV in multiplex as well as for a real-time detection, whereby at least the thirteen HR HPV can be detected in a single-tube assay. The invention further allows for a reliable quantitation of HPV viral loads in real-time multiplex amplification. | 02-20-2014 |
20140051067 | PROCESS FOR THE DETECTION AND EARLY SEROTYPING OF THE DENGUE VIRUS - Disclosed is a process for in vitro detection of an infection by a dengue virus in an individual, including the following steps: contacting a blood sample from the individual with a ligand specific to the NS1 protein of said dengue virus, to capture NS1 protein if it is present in the blood sample, said ligand being immobilized on a solid support; detecting the presence of NS1 protein via a mass spectrometry reading; and if NS1 protein is detected, concluding that the individual has been infected by dengue virus. | 02-20-2014 |
20140057247 | METHOD OF PREPARING RNA FROM RIBONUCLEASE-RICH SOURCES - Methods for preparing RNA from ribonuclease-rich sources while avoiding RNA degradation are described. The lysis protocol for ribonuclease-containing samples is performed at high pH to accelerate cell lysis and with a reducing agent that inactivates ribonucleases (RNases) by reducing disulfide bonds essential for RNase activity. Samples are briefly incubated for up to five minutes at high pH followed by addition of a reagent to lower the pH to a level at which the RNA is stable. This method of RNA extraction has many advantages over existing methods of RNA preparation, including that cell lysis is efficient, RNases are rapidly inactivated, and sample incubation times are short (less than 5 minutes), which protects RNA from degradation. The lysing procedure is performed entirely in aqueous solution with no heating, precipitations, or buffer exchanges required. Thus, a quick, simple procedure for extracting RNA is provided, which can easily be automated. | 02-27-2014 |
20140057248 | FLUORESCENCE ENERGY TRANSFER BY COMPETITIVE HYBRIDIZATION - A method is provided for detecting the presence of nucleotides or monitoring nucleotide amplification. It utilizes fluorescence energy transfer by competitive hybridization. Competitive hybridization is achieved by using unequal length complementary probes which have a fluorophore on one probe and a quencher on the other. The fluorophore and quencher are juxtaposed in a manner wherein the proximity of the quencher to the fluorophore produces quenching of the fluorescence of the fluorophore. | 02-27-2014 |
20140057249 | JAK/STAT INHIBITORS AND MAPK/ERK INHIBITORS FOR RSV INFECTION - The present invention concerns a method for treating or reducing the likelihood of developing a respiratory syncytial virus (RSV) infection in a subject by administering an effective amount of an inhibitor of the janus kinase (JAK)/signal transducer and activator of transcription (STAT) signaling pathway or the mitogen-activated kinase (MAPK)/extracellular signal-regulated kinase (ERK1/2) signaling pathway to the subject. Another aspect of the invention concerns a pharmaceutical composition that includes an inhibitor of JAK/STAT or MAPK/ERK signaling to the subject; and a pharmaceutically acceptable carrier. Another aspect of the invention concerns a method for identifying agents useful for treating or reducing the likelihood of developing an RSV infection | 02-27-2014 |
20140065598 | SELF-CONTAINED BIOLOGICAL ASSAY APPARATUS, METHODS, AND APPLICATIONS - A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing. | 03-06-2014 |
20140065599 | NOVEL POLYMAVIRUS ASSOCIATED WITH DIARRHEA IN CHILDREN - Provided is a new polyomavirus, provisionally named MX polyomavirus, (MXPyV). Further provided are cDNA nucleic acid sequences, recombinant proteins, expression vectors and host cells, recombinant anti-MXPyV antibodies, vaccines, compositions, methods of detecting MXPyV, methods for assaying for anti-MXPyV compounds, and methods for treating or preventing a MXPyV infection. | 03-06-2014 |
20140065600 | METHOD OF EVALUATING ORAL CANCER RISK IN HUMAN - A method of providing a risk evaluation and diagnosis of human oral cancer, by examining at the presence in human saliva sample of a combination of particulate nucleic acids from bacteria, virus, as well as human, and/or the presence of particulate biochemical volatile organic compounds, which are indicative of an increased risk of oral cancer. | 03-06-2014 |
20140065601 | METHOD AND KIT FOR ESTIMATING HUMAN IMMUNODEFICIENCY VIRUS (HIV) INCIDENCE - The present invention provides a method for determining the incidence of human immunodeficiency virus (HIV) infections in a population comprising comparing the anti-HIV antibody levels of in vitro stimulated tissue samples to those of un-stimulated tissue samples from individual members of said population and related kits. The present invention also provides a method of determining the distribution of recent, non-recent, and late stage human immunodeficiency virus (HIV) infections in a population comprising comparing the in vitro stimulated anti-HIV immunoreactivity and un-stimulated anti-HIV immunoreactivity in tissue samples from individual members of said population and related kits. | 03-06-2014 |
20140065602 | Human Exhaled Aerosol Droplet Biomarker System and Method - A system and method for detecting a biomarker in exhaled breath condensate nanodroplets comprises noninvasively collecting exhaled breath condensate nanodroplets of a subject, and analyzing said nanodroplets utilizing immuno-quantitative polymerase chain reaction to detect one or more target biomarkers. | 03-06-2014 |
20140065603 | Quantitative Real-Time Assay for Noroviruses and Enteroviruses with Built in Quality Control Standard - A method is provided for reverse transcription-polymerase chain reaction (RT-PCR) comprising a) amplifying a reverse transcribed cDNA in a mixture comprising Norovirus Genogroup I and Norovirus Genogroup II primers and probes, wherein said Norovirus primers and probes distinguish between Genogroup I and Genogroup II viruses; b) quantifying virus; and c) normalizing data based on a universal internal RNA control. Optionally, the method may also include primers and probes for Enteroviruses. A reaction mixture comprising Norovirus Genogroup I and Norovirus Genogroup II primers and probes, wherein said Norovirus primers and probes distinguish between Genogroup I and Genogroup II viruses and universal internal RNA control primers and probes are also included. | 03-06-2014 |
20140072956 | COMPOSITION, DEVICE AND ASSOCIATED METHOD - A composition includes a first probe capable of binding to a first analyte and a first initiator bonded to the first probe. The composition further includes a second probe capable of binding to a second analyte and a second initiator bonded to the second probe. At least one of the first initiator or the second initiator is capable of initiating a controlled polymerization reaction. An associated kit, device, and method are provided. | 03-13-2014 |
20140072957 | GENETIC POLYMORPHISMS ASSOCIATED WITH LIVER FIBROSIS, METHODS OF DETECTION AND USES THEREOF - The present invention is based on the discovery of genetic polymorphisms that are associated with liver fibrosis and related pathologies. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, variant proteins encoded by such nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and proteins, and methods of using the nucleic acid and proteins as well as methods of using reagents for their detection. | 03-13-2014 |
20140072958 | NOVEL INFLUENZA HEMAGGLUTININ PROTEIN-BASED VACCINES - Novel vaccines are provided that elicit broadly neutralizing anti-influenza antibodies. Some vaccines comprise nanoparticles that display hemagglutinin trimers from influenza virus on their surface. The nanoparticles comprise fusion proteins comprising a monomeric subunit of ferritin joined to at least a portion of an influenza hemagglutinin protein. Some portions comprise the ectodomain while some portions are limited to the stem region. The fusion proteins self-assemble to form the hemagglutinin-displaying nanoparticles. Some vaccines comprise only the stem region of an influenza hemagglutinin protein joined to a trimerization domain. Such vaccines can be used to vaccinate an individual against infection by heterologous influenza viruses and influenza virus that are antigenically divergent from the virus from which the nanoparticle hemagglutinin protein was obtained. Also provided are fusion proteins and nucleic acid molecules encoding such proteins. Finally, also provided are assays using nanoparticles of the invention to detect anti-influenza antibodies. | 03-13-2014 |
20140072959 | RAPID TESTS FOR INSURANCE UNDERWRITING - The present invention relates to rapid test devices, kits and systems comprising the rapid test devices for assessing at least two analytes in a sample derived from a life or health insurance applicant, said at least two analytes being selected from the group consisting of a human immunodeficiency virus (HIV) antigen (e.g., a HIV polypeptide), a HIV polynucleotide, an anti-HIV antibody, a hepatitis C virus (HCV) antigen (e.g., a HCV polypeptide), a HCV polynucleotide, an anti-HCV antibody, a liver enzyme alanine transaminase (ALT), a liver enzyme aspartate transaminase (AST), nt-probnp (or NT-proBNP), probnp (or proBNP), cotinine, nicotine, cocaine, a metabolite of cocaine (e.g., benzoylecgonine), prostate-specific antigen (PSA), apolipoprotein A-1 (ApoA1), apolipoprotein B (ApoB), Hemoglobin A1c and high-sensitivity C-reactive protein (hsCRP). The present invention also relates to methods for using the rapid test devices, kits and systems as part of the assessment of the life or health insurance applications. | 03-13-2014 |
20140072960 | SELF DIAGNOSTIC TEST - A test unit collects and analyzes biological specimens on-site. It has one or more openings that allow reagent capsules to be inserted and guided into a testing chamber. Reagent capsules are preloaded with chemicals for screening and are configured as blister packs. A button mechanism allows projection(s) to open the blister packs to allow chemicals within the capsules to mix inside the testing chamber. A medical swab is affixed to a pop top dispenser cap and can be pressed to allow the swab to be inserted into the mixed chemicals. A visible test strip mount attached to the testing chamber has a lever to manipulate a test strip in and out of the testing chamber. This test strip may be slid through a uni-directional seal on a capsule and into a chemical for testing. The test strip will test for the presence of an infectious disease. | 03-13-2014 |
20140072961 | Method of Genome Surgery with Paired, Permeant Endonuclease Excision - The use of P2E2 constructs in genome surgery includes a cell penetration component, a DNA binding component and a restriction endonuclease. The method for performing genome surgery includes:
| 03-13-2014 |
20140072962 | SYSTEMS, DEVICES, AND METHODS FOR IDENTIFYING A DISEASE STATE IN A BIOLOGICAL HOST USING INTERNAL CONTROLS - Contemplated methods and devices comprise detecting the presence of a pathogen in a biological host. In certain implementations, a sample is provided from a biological host. A biosensor is provided, the biosensor having a first probe configured to detect a control marker in the sample, the control marker being an endogenous element of the biological host. The biosensor has a second probe configured to detect the presence of a target marker in the sample, the target marker being from a pathogen in the biological host. The sample is applied to the biosensor, and the presence or absence of the control marker in the sample is identified using the first probe. The presence or absence of the target marker in the sample is identified using the second probe. | 03-13-2014 |
20140072963 | ELECTROCHEMILUMINESCENCE IMMUNOASSAY METHOD - Disclosed is an electrochemiluminescence immunoassay method, using a full reaction of a Ru(bpy) marked protein-primary antibody, a biotinylated protein-secondary antibody to be tested, and a sample to be tested; addition of a Streptavidin-coated magnetic particle to form a complex comprising an antigen, an antibody, and a magnetic particle; adsorption to an electrode surface by the magnetic particle; addition of a dibutyl ethanolamine solution; testing by means of an electrochemical method. Also disclosed is a corresponding electrochemiluminescence immunoassay detection kit. | 03-13-2014 |
20140072964 | Cooperating Capillary and Cap for Use in a High-Pressure Freezer - The invention relates to an assembly of a cooperating capillary and cap for containing an aqueous solution in an inner volume of the capillary. The assembly is used in a high pressure freezer in which the aqueous solution is frozen at a high pressure to form an amorphous frozen sample at a cryogenic temperature. The cap forms a closure at one end of the capillary, and the part of the cap that is in contact with the inner volume of the capillary has an indent; as a result of which the cap, after freezing the aqueous solution, can be removed from the capillary and a free standing pillar of frozen aqueous material extends from the capillary. | 03-13-2014 |
20140080118 | SAMPLE ASSEMBLY WITH AN ELECTROMAGNETIC FIELD TO ACCELERATE THE BONDING OF TARGET ANTIGENS AND NANOPARTICLES - Described are embodiments of an invention for a sample assembly with an electrical conductor for generating an electromagnetic field to speed up the tagging of target antigens with antiparticles for detection of the antigens by electromagnetic read heads. A sample assembly includes a surface with a first set of antibodies bonded thereon. Target antigens are bonded with the first set of antibodies. A second set of antibodies bonded to nanoparticles are exposed to the sample surface to bond with the target antigens. The electrical conductor generates an electromagnetic field that moves the nanoparticle-labeled antibodies toward the antigens to shorten the time to complete their bonding process. | 03-20-2014 |
20140080119 | MOLECULAR NETS - A molecular net formed as a branched pseudorandom copolymer including two broad classes of subunits: capture agents and linking agents. The subunits self-assemble to form a structure capable of binding to predetermined targets. The binding can then be detected. | 03-20-2014 |
20140080120 | DETECTION OF ANTIBODIES USING AN IMPROVED IMMUNE COMPLEX (IC) ELISA - The present invention relates to the field of diagnostic or analytic methods. In particular, the inventors teach that FcγR such as CD16, CD32 or CD64 may be used for in vitro quantification of antibodies in the form of immune complexes, i.e., complexes formed by antigen and specific antibody. A method for detection and quantification of antibodies in the form of immune complexes is also provided. | 03-20-2014 |
20140080121 | PRIMATE T-LYMPHOTROPIC VIRUSES - Disclosed are compositions and methods related to the isolation and identification of the primate T-lymphotropic viruses, HTLV-3 and HTLV-4. The diversity of HTLVs was investigated among central Africans reporting contact with NHP blood and body fluids through hunting, butchering, and keeping primate pets. Herein it is shown that this population is infected with a variety of HTLVs, including two retroviruses; HTLV-4 is the first member of a novel phylogenetic lineage that is distinct from all known HTLVs and STLVs; HTLV-3 falls within the genetic diversity of STLV-3, a group that has not previously been seen in humans. The present disclosure also relates to vectors and vaccines for use in humans against infection and disease. The disclosure further relates to a variety of bioassays and kits for the detection and diagnosis of infection with and diseases caused by HTLV-3 and HTLV-4 and related viruses. | 03-20-2014 |
20140087362 | Methods for assessing effectiveness and monitoring oncolytic virus treatment - Diagnostic methods for in vivo and ex vivo detection of circulating tumor cells (CTCs) for the diagnosis and treatment of cancer are provided. The diagnostic methods employ oncolytic viruses alone or in combination with one or more tumor cell enrichment and/or detection methods. Combinations and kits for use in the practicing the methods also are provided. | 03-27-2014 |
20140087363 | METHOD FOR GENERATING, STORING, TRANSPORTING, ELUTING AND DETECTING CLINICAL RELEVANT INFORMATION IN PLASMA USING FILTER PAPER - The present invention relates to a method that enables simpler, easier and more accurate determination cell mediated immune (CMI) responses using the biomarker IP-10 together with a simple and safe “dried blood spot” filter paper method of storing and shipping samples. The method is useful for the diagnosis and prognostication of diseases and conditions that can be diagnosed and prognosticated by measuring correlates of IP-10. | 03-27-2014 |
20140087364 | METHOD AND KIT FOR DETERMINING THE TIME OF SEROCONVERSION OF A PATIENT INFECTED WITH A VIRUS - The present invention provides a method for determining the time of infection, and a method for determining if a microbial infection is in the early stages comprising the step of determining the ratio of in vitro stimulated anti-microbial immunoreactivity and un-stimulated anti-microbial immunoreactivity in blood samples from said subject and related kits. | 03-27-2014 |
20140087365 | IMMUNOCHROMATOGRAPHIC DETECTION METHOD CAPABLE OF DETERMINING SAMPLE WITHOUT SPECIMEN AS IMPROPERLY OPERATED SAMPLE AND TEST STRIP USED THEREWITH - An object of the present invention is to provide an immunochromatographic detection method comprising a step of detecting the failure to add a specimen and an immunochromatographic test strip comprising a means for detecting the failure to add a specimen. The inventers provides an immunochromatographic detection method and an immunochromatographic test strip capable of detecting the failure to add a specimen through a step and a means using an antibody solid-phased on an insoluble membrane for capturing the complex of a component (control component) contained in the specimen other than the analyte and a label to which an antibody to the component is immobilized so as to detect the presence or absence of the control component, in an immunochromatographic detection method of detecting an analyte in a sample acquired by diluting the specimen. | 03-27-2014 |
20140087366 | USE OF DIVALENT IONS, PROTEASES, DETERGENTS, AND LOW pH IN THE EXTRACTION OF NUCLEIC ACIDS - The present invention provides methods of extracting target nucleic acids from a biological sample using divalent salts and acidic conditions. | 03-27-2014 |
20140087367 | CHROMATOGRAPHY METHOD AND CHROMATOGRAPHIC KIT - The chromatography method includes a step of forming a composite with a test substance and a labeling substance containing a metal modified by a first binding substance of the test substance and then developing the composite on an insoluble carrier; a step of capturing the test substance and the labeling substance in a detection site on the insoluble carrier including a second binding substance of the test substance or a substance having a binding property to the first binding substance of the test substance; and a step of amplifying the captured labeling substance using a first amplification reagent and a second amplification reagent to detect the test substance. | 03-27-2014 |
20140087368 | Methods for Antimicrobial Resistance Determination - The present invention relates to methods and systems for determining the antibiotic-resistance status of microorganisms. The invention further provides methods for determining the antibiotic-resistance status of microorganisms in situ within a single system. | 03-27-2014 |
20140087369 | INFECTION DETECTION METHODS AND SYSTEMS AND RELATED COMPOUNDS AND COMPOSITIONS - A compound, or a pharmaceutically acceptable salt, ester, hydrate or solvate thereof, comprising formula I: | 03-27-2014 |
20140093865 | LATERAL FLOW ASSAYS FOR TAGGED ANALYTES - Methods of determining whether an analyte is present in a sample using a competitive assay are provided. Aspects of the methods include employing a competitor that provides for the obtainment of signal which is directly proportional to the amount of analyte in the sample. Also provided are devices and kits that find use in practicing the methods described herein. | 04-03-2014 |
20140093866 | FLUID MIXING AND DELIVERY IN MICROFLUIDIC SYSTEMS - The specification generally discloses systems and methods for mixing and delivering fluids in microfluidic systems. The fluids can contain, in some embodiments reagents that can participate in one or more chemical or biological reactions. Some embodiments relate to systems and methods employing one or more vent valves to controllably flow and/or mix portions of fluid within the microfluidic system. Advantageously, fluid control such as a sequence of fluid flow and/or a change in flow rate, can be achieved by opening and closing one or more vent valves and by applying a single source of fluid flow (e.g., a vacuum) operated at a substantially constant pressure. This can simplify the operation and use of the device by an intended user. | 04-03-2014 |
20140093867 | PARTICLE SEPARATION AND CONCENTRATION USING SPIRAL INERTIAL FILTRATION - A spiral inertial filtration device is capable of high-throughput (1 mL/min), high-purity particle separation while concentrating recovered target particles by more than an order of magnitude. Large fractions of sample fluid are removed from a microchannel without disruption of concentrated particle streams by taking advantage of particle focusing in inertial spiral microfluidics, which is achieved by balancing inertial lift forces and Dean drag forces. To enable the calculation of channel geometries in the device for specific concentration factors, an equivalent circuit model was developed and experimentally validated. Large particle concentration factors were achieved by maintaining either average fluid velocity or Dean number throughout the entire length of the channel during the incremental removal of sample fluid. Also provided is the ability to simultaneously separate more than one particle from the same sample. | 04-03-2014 |
20140093868 | CHROMATOGRAPHY METHOD - A chromatographic kit is provided including a labeling substance holding area having a labeling substance modified with a first binding substance of a test substance, and a labeling substance capturing area having a second binding substance of the test substance or a binding substance of the first binding substance in this order from upstream to downstream of a development direction of a test sample including the test substance, and further including an area having a color developing reagent in order to detect a first amplification reagent of two types of amplification reagents used to amplify the signal of the labeling substance when detecting the labeling substance. | 04-03-2014 |
20140099630 | QUANTITATIVE DETERMINATION METHOD FOR TARGET PARTICLES, PHOTOMETRIC ANALYSIS DEVICE, AND COMPUTER PROGRAM FOR PHOTOMETRIC ANALYSIS - The method of the present invention includes: preparing a sample solution containing the target particles and luminescent probes to be bound to the target particles, and binding these in the sample solution; moving a position of a light detection region of the optical system in the sample solution using a confocal microscope or a multiphoton microscope, and detecting light signal emitted from the luminescent probe in the light detection region while moving the position of the light detection region, and individually detecting the target particles directly or indirectly; and counting the number of the detected target particles, and calculating the concentration of the target particles in the sample solution from the number of the counted target particles on the basis of a calibration curve that approximates the correlation between the concentration or quantity of the target particles in the sample solution and the number of the target particles. | 04-10-2014 |
20140099631 | APPARATUS AND METHOD FOR PROCESSING BIOLOGICAL SAMPLES AND A RESERVOIR THEREFOR - An apparatus for processing at least one biological sample accommodated on at least one carrier member ( | 04-10-2014 |
20140106334 | MONITORING AND INHIBITING HUMAN IMMUNODEFICIENCY VIRUS INFECTION BY MODULATING HMGB1 DEPENDENT TRIGGERING OF HIV-1 REPLICATION AND PERSISTENCE - Compositions and methods for modulating human immunodeficiency virus (HIV) infection involving substances that inhibit the ability of high mobility box 1 (HMGB1) protein to interact with natural killer (NK) cells. Therapeutic compositions comprising antibodies and drugs, such as glycyrrhizin, which bind to HMGB1. Methods of detecting or monitoring HIV infection involving detection or quantitation of HMGB1 or antibodies specific for HMGB1 in a biological sample. | 04-17-2014 |
20140106335 | OLIGONUCLEOTIDE PROBE FOR THE DETECTION OF ADENOVIRUS - The present invention relates to an oligonucleotide probe for the detection of adenoviral nucleic acid in a biological sample. In a further aspect, the invention relates to a method for the detection of an adenoviral nucleic acid in a biological sample. The invention also provides oligonucleotide primers for amplifying adenoviral nucleic acid. Kits comprising probes and/or primers of the invention are also provided. Finally, the invention also relates to the use of the oligonucleotide primers and/or probes for detecting adenoviral nucleic acid. | 04-17-2014 |
20140106336 | Detection of Magnetic-Field-Concentrated Analytes in a Lateral Flow Capillary - The present disclosure generally relates to systems, devices and methods for detecting magnetic-field-concentrated target analytes within a lateral flow capillary. | 04-17-2014 |
20140106337 | METHODS, NUCLEIC ACIDS, AND KITS FOR DETECTION OF PARVOVIRUS B19 - Nucleic acid molecules derived from sequences of novel human parvovirus B19 variant genomes are provided. Also provided are assays and kits comprising the nucleic acid molecules. | 04-17-2014 |
20140106338 | LOCALIZED DESALTING SYSTEMS AND METHODS - Example apparatus, systems and methods to desalt a sample are disclosed. An example apparatus includes a substrate and a sensor disposed on the substrate. The sensor has a surface functionalized with a binding agent to interact with an analyte in a liquid sample when the liquid sample is in contact with the sensor surface. The example apparatus further includes an electrode disposed on the substrate to create an electric potential and move ions in the sample away from the surface of the sensor. | 04-17-2014 |
20140106339 | METHODS AND MATERIALS FOR DETECTING CONTAMINATED FOOD PRODUCTS - This document provides methods and materials for detecting contaminated food products. For example, methods and materials for using an enzymatic amplification cascade of restriction endonucleases to detect nucleic acid of a microorganism or virus (e.g., a pathogen) within a sample (e.g., food product sample) being tested, thereby assessing a food product for possible contamination are provided. | 04-17-2014 |
20140106340 | HIV-1 GENOTYPING ASSAY FOR GLOBAL SURVEILLANCE OF HIV-1 DRUG RESISTANCE - Provided herein are new methods, primers, and kits for genotyping HIV-1, including group M viral strains. The methods can be used for HIV-1 drug resistance surveillance and monitoring, for example in resource-poor countries. The disclosed methods can detected more mixed HIV-1 population than previous methods. Given the high efficiency in genotyping diverse HIV-1 group M viral strains from plasma and dried blood spot (DBS) samples and substantial reagent cost saving, the disclosed methods can be used for HIV-1 drug resistance genotyping in both antiretroviral therapy (ART)-naive and -experienced populations for surveillance purposes and patient monitoring. | 04-17-2014 |
20140113277 | ULTRASONIC BIOLOGICAL SAMPLE ANALYSIS APPARATUS AND METHODS - An ultrasonic sample evaluation process in which a vial containing a liquid medium is placed on a receptacle and a ultrasonic source signal is propagated upwards in the vertical direction through the liquid medium. A number of reflected ultrasonic signals is received at the bottom of the sample vial and evaluated to identify a pattern characteristic of a reflection off the meniscus, and the presence of a pattern characteristic of a collection implement located in the liquid medium. An estimate of the amount of liquid medium contained in the sample vial is generated based on a time characteristic of the pattern characteristic of a reflection off the meniscus, and a signal indicating the presence of a collection implement in the liquid medium is generated upon identifying such a pattern. | 04-24-2014 |
20140113278 | SAMPLE PROCESSING APPARATUS AND METHODS - An automated process for converting samples includes: receiving tube strips having a number of sample tubes and samples therein, transferring multiple tube strips to a tube strip holder, dispensing sample conversion buffer into each tube, shaking the tube strip holder a first time, centrifuging the tube strip holder, removing a liquid supernatant from each tube, simultaneously inspecting the contents of each tube, dispensing a specimen transport medium and a denaturation reagent into each tube, shaking the tube strip holder a second time, heating the tube strip holder for a first length of time, shaking the tube strip holder a third time, heating the tube strip holder for a second length of time, shaking the tube strip holder a fourth time, and transferring at least a portion of each sample to a respective well on an output plate. | 04-24-2014 |
20140113279 | Method for Preventing High Molecular Weight Products During Amplification - The present invention is directed to improved methods for amplifying and detecting a nucleic acid target that may be present in a biological sample comprising a primer pair for generating an amplicon, wherein at least one primer is modified at the 5′ terminus with a polyN sequence being non-complementary to the target sequence, and a detectable probe specific for said amplicon or a DNA binding dye. The formation of high molecular weight products during amplification is prevented or partly or completely suppressed. The invention further provides reaction mixtures and kits comprising said primers for preventing or suppressing the formation of high molecular weight products during amplification and detection of the nucleic acid target. | 04-24-2014 |
20140113280 | DEVICE FOR DETECTION OF MOLECULES IN BIOLOGICAL FLUIDS - Disclosed is a test device and a method for qualitatively and/or quantitatively measuring the concentration of an analyte in a biological fluid sample. The test device includes a housing defining a sample port, a test well containing a stirrer and a conjugate, and a test strip disposed within the housing. The test well is also defined by being located between the sample port and the test strip. Fluid flows from the test well onto the test strip, which has a trapping zone which binds the analyte and allows for its detection. A control zone may also be included. The test device is generally adapted to use a sandwich assay. Also disclosed is a system comprising the test device and a signal sensing device; and a method for using the test device. | 04-24-2014 |
20140120522 | NANOFLUIDIC DEVICES FOR SINGLE-MOLECULE ANALYSIS OF PROTEIN-DNA COMPLEX - A device for optical mapping of protein binding sites, in particular, transcription factor binding sites, on single DNA molecules, includes an insulating substrate having two parallel channels and at least one slit connecting the two channels, a coverslip on the substrate, at least two reservoirs on the substrate connecting the channels of the insulating substrate, and at least two electrodes in the reservoirs. When the reservoirs are filled with a buffer solution, the electrodes are in electrical contact in the buffer solution. | 05-01-2014 |
20140120523 | NMR SYSTEMS AND METHODS FOR THE RAPID DETECTION OF ANALYTES - This invention features systems and methods for the detection of analytes, and their use in the treatment and diagnosis of disease. | 05-01-2014 |
20140120524 | In-Situ Pathogen Detection Using Magnetoelastic Sensors - In at least one illustrative embodiment, a method for in-situ pathogen detection may comprise distributing one or more magnetoelastic measurement sensors on a surface of a test object, wherein each of the one or more magnetoelastic measurement sensors includes a biorecognition element configured to bind with a pathogen to cause a shift in a characteristic frequency of the associated measurement sensor; applying a varying magnetic field, using a test coil, to the one or more magnetoelastic measurement sensors distributed on the surface of the test object, wherein the test object is positioned outside of an inner volume defined by the test coil; detecting a frequency response of the one or more magnetoelastic measurement sensors using the test coil, while applying the varying magnetic field; and determining whether the pathogen is present based on the detected frequency response of the one or more magnetoelastic measurement sensors. | 05-01-2014 |
20140120525 | MONITORING AND INHIBITING HUMAN IMMUNODEFICIENCY VIRUS INFECTION BY MODULATING HMGB1 DEPENDENT TRIGGERING OF HIV-1 REPLICATION AND PERSISTENCE - Compositions and methods for modulating human immunodeficiency virus (HIV) infection involving substances that inhibit the ability of high mobility box 1 (HMGB1) protein to interact with natural killer (NK) cells. Therapeutic compositions comprising antibodies and drugs, such as glycyrrhizin, which bind to HMGB1. Methods of detecting or monitoring HIV infection involving detection or quantitation of HMGB1 or antibodies specific for HMGB1 in a biological sample. | 05-01-2014 |
20140120526 | METHODS, COMPOSITIONS, AND KITS FOR DETERMINING HUMAN IMMUNODEFICIENCY VIRUS (HIV) - The present invention relates to compositions, methods, and kits for determining the presence or absence of HIV in a sample, in particular for determining HIV-1 group M, HIV-1 group O, and/or HIV-2, in particular for simultaneous determining of HIV-1 group M, HIV-1 group O, and HIV-2. | 05-01-2014 |
20140127671 | Simultaneous Diagnosis Kit For a Disease Due to a Respiratory Virus - The present invention relates to a kit for simultaneous diagnosis of viral respiratory diseases. To be more specific, the present invention is directed to a method for diagnosing viral respiratory diseases by detecting the genes specific to the respiratory disease-causing virus, a primer set for diagnosing the viral respiratory diseases used in the diagnosis method, a composition for simultaneous diagnosis of viral respiratory diseases, comprising the primer set, and a kit for simultaneous diagnosis of viral respiratory diseases, comprising the composition. When the primer set of the present invention for diagnosing the viral respiratory diseases is used, 14 different types of respiratory viruses can be simultaneously detected only with one reaction through real-time multiplex reverse transcription (RT)-PCR, and the onset of respiratory diseases caused by these viruses can be diagnosed. Thus, the primer set of the present invention can be widely used for prompt diagnosis and treatment of respiratory diseases. | 05-08-2014 |
20140127672 | DISEASE DETECTION IN PLANTS - The present disclosure relates to disease detection in plants. In particular, it provides methods, compositions, and devices for the detection of diseases in plants. | 05-08-2014 |
20140127673 | DUAL PROBE ASSAY FOR THE DETECTION OF HCV - The present invention relates to a method for amplifying and detecting a target nucleic acid of HCV in a sample, wherein an amplification of the nucleic acids in said sample is carried out. This amplification involves a polymerase, primers for generating an amplicon and at least two detectable probes specific for different sequence portions of said amplicon. Detection of the obtained amplicon is brought about by detecting hybridization of the probes mentioned above to said different sequence portions of the amplicon. | 05-08-2014 |
20140127674 | OPTIMIZED PROBES AND PRIMERS AND METHODS OF USING SAME FOR THE BINDING, DETECTION, DIFFERENTIATION, ISOLATION AND SEQUENCING OF INFLUENZA A; INFLUENZA B AND RESPIRATORY SYNCYTIAL VIRUS - Described herein are primers and probes useful for the binding, detecting, differentiating, isolating, and sequencing of influenza A, influenza B and RSV viruses. | 05-08-2014 |
20140127675 | MATERIALS AND METHODS FOR DETECTING TOXINS, PATHOGENS AND OTHER BIOLOGICAL MATERIALS - Embodiments of the present invention provide binding molecule-functionalized high electron mobility transistors (HEMTs) that can be used to detect toxins, pathogens and other biological materials. In a specific embodiment, an antibody-functionalized HEMT can be used to detect botulinum toxin. The antibody can be anchored to a gold-layered gate area of the | 05-08-2014 |
20140127676 | ISOLATED LIVER STEM CELLS - A method of conducting in vitro toxicity testing is disclosed which includes the steps of exposing to a test agent a population of human liver progenitor or stem cells originated from human adult liver. The cells have the characteristics that the isolated human progenitor or stem cells express at least the mesenchymal markers vimentin and α-smooth muscle actin (ASMA), the hepatocyte marker albumin (ALB), are negative for cytokeratin-19 (CK-19), and have mesenchymal-like morphology. One or more effects, if any, of the test agent on the population of human liver progenitor or stem cells are observed. Also disclosed are methods of conducting in vitro drug metabolism studies by exposing to a test agent a population of the human liver progenitor or stem cells and methods of testing infected human liver progenitor or stem cells for effects of a test agent. | 05-08-2014 |
20140134600 | BIOMARKERS ASSOCIATED WITH DEVELOPMENT OF HEPATOCELLULAR CARCINOMA IN PATIENTS WITH HEPATITIS B VIRUS INFECTION, AND METHOD FOR DETECTION THEREOF - The present invention is intended to provide a method for predicting risk of hepatocellular carcinoma (HCC) in hepatitis B virus (HBV)-infected patients with a high accuracy. More specifically, the invention provides a method for detecting eight mutations of HBV genome associated with predisposition to HCC, comprising: C1653T, A1762T, G1764A, T1674C, T1753C, C3116T, T53C and A1846T mutations, and primers and probes sets used thereof consist of SEQ ID NO: 1-SEQ ID NO: 24. | 05-15-2014 |
20140134601 | USE OF PROTEIN NANOPARTICLE BASED HYDROGEL - The present invention relates to a use of a protein nanoparticle-based hydrogel, and more particularly, to a use of a protein nanoparticle-based hydrogel capable of highly sensitive and simultaneous multi-detection of disease markers by using a hydrogel to which a protein nanoparticle representing a disease marker detection probe is immobilized. | 05-15-2014 |
20140134602 | SUBSTANCE DETERMINING APPARATUS - The invention relates to a substance determining apparatus ( | 05-15-2014 |
20140134603 | ASSAY DEVICE AND METHOD - An assay method is described, which comprises the steps of immobilizing a binding partner (e.g., an antigen or antibody) for an analyte to be detected (e.g., an antibody or antigen) on a portion of a surface of a microfluidic chamber; passing a fluid sample over the surface and allowing the analyte to bind to the binding partner; allowing a metal colloid, e.g., a gold-conjugated antibody, to associate with the bound analyte; flowing a metal solution, e.g., a silver solution, over the surface such as to form an opaque metallic layer; and detecting the presence of said metallic layer, e.g., by visual inspection or by measuring light transmission through the layer, conductivity or resistance of the layer, or metal concentration in the metal solution after flowing the metal solution over the surface. | 05-15-2014 |
20140134604 | HCV GENOTYPE 6 REPLICONS - Replicons of genotype 6 hepatitis C virus (HCV) are provided. These replicons contain adaptive mutations giving rise to the HCV's capability to replicate in vitro. Methods of preparing genotype 6 replicons and methods of using these replicons to screen antiviral agents are also provided. | 05-15-2014 |
20140134605 | Reagents and methods for detecting HCV - The present disclosure relates to oligonucleotide sequences for amplification primers and their use in performing nucleic acid amplifications of HCV, in particular regions that encode the NS3 polypeptide. In some embodiments the primers are used in nested PCR methods for the detection or sequencing of HCV NS3. The oligonucleotide sequences are also provided assembled as kits that can be used to amplify and detect or sequence HCV NS3. | 05-15-2014 |
20140134606 | EXOSOME-MEDIATED DIAGNOSIS OF HEPATITIS VIRUS INFECTIONS AND DISEASES - A method for diagnosing hepatitis virus infection or a hepatitis disease condition in a subject based on hepatitis virus-associated biomarkers present on exosomes in a bodily fluid sample from the subject is disclosed. Also disclosed are a method for monitoring the course of a hepatitis virus infection or a hepatitis disease condition in a subject and a method for monitoring effectiveness of treatment to a subject with an anti-hepatitis virus agent based on hepatitis virus-associated biomarkers present on exosomes in bodily fluid samples from the subject, as well as a kit for diagnosing hepatitis virus infection and/or a hepatitis disease condition in a subject based on hepatitis virus-associated biomarkers on exosomes in bodily fluid samples from the subject. | 05-15-2014 |
20140134607 | MEMS AFFINITY SENSOR FOR CONTINUOUS MONITORING OF ANALYTES - Techniques for monitoring a target analyte in a sample using a polymer capable of binding to the target analyte are disclosed. A microdevice useful for the disclosed techniques includes a semi-permeable membrane structure, a substrate, a first and second microchambers formed between the membrane structure and the substrate. The first microchamber can be adapted to receive a solution including the polymer, and the second microchamber can be adapted to receive a reference solution. Environmental target analyte can permeate the semi-permeable membrane structure and enters the first microchamber and the second microchamber. Based on the difference in a property associated with the polymer solution that is responsive to the target analyte-polymer binding, and the corresponding property associated with reference solution, the presence and/or concentration of the target analyte can be determined. | 05-15-2014 |
20140134608 | SINGLE PARTICLE DETECTION DEVICE, SINGLE PARTICLE DETECTION METHOD, AND COMPUTER PROGRAM FOR SINGLE PARTICLE DETECTION, USING OPTICAL ANALYSIS - There is provided a single particle detection technique based on a scanning molecule counting method, enabling individual detection of a single particle using light measurement with a confocal or multiphoton microscope, and quantitative observation of conditions or characteristics of the particle. The inventive technique of detecting a single particle in a sample solution detects light containing substantially constant background light from a light detection region with moving the position of the light detection region of the microscope in a sample solution to generate time series light intensity data; and detects individually a light intensity reduction occurred when a single particle which does not emit light (or a particle whose emitting light intensity in a detected wavelength band is lower than the background light) enters in the light detection region in the time series light intensity data as a signal indicating the existence of each single particle. | 05-15-2014 |
20140141406 | BIOMARKERS FOR PRENATAL DIAGNOSIS OF CONGENITAL CYTOMEGALOVIRUS - The invention provides compositions and methods useful for early detection of congenital CMV infection, predicting the likelihood and severity of congenital CMV disease, and monitoring the efficacy of therapeutic approaches. Compositions of the present invention include biomarkers that are differentially expressed in CMV-infected mothers and fetuses compared to uninfected individuals. | 05-22-2014 |
20140141407 | METHODS AND MATERIALS FOR DETECTING VIRAL OR MICROBIAL INFECTIONS - This document provides methods and materials for detecting target nucleic acid. For example, methods and materials for detecting the presence or absence of target nucleic acid, methods and materials for detecting the amount of target nucleic acid present within a sample, kits for detecting the presence or absence of target nucleic acid, kits for detecting the amount of target nucleic acid present within a sample, and methods for making such kits are provided. | 05-22-2014 |
20140141408 | INTEGRATED SENSOR FOR THE RAPID IDENTIFICATION OF BACTERIA USING ISFETS - Disclosed are methods and systems for the detection of bacteria in a sample. The methods comprises contacting the sample with an antibacterial agent and a bacteria identification sensor, and involves the permeabilization of the bacteria by the antibacterial agent, and the subsequent detection of an efflux of potassium ions using a bacteria identification sensor comprising a potassium-sensitive ISFET. Also disclosed are bacteria identification sensor comprising a potassium-sensitive ISFET useful in the practice of the disclosed methods. | 05-22-2014 |
20140141409 | REAGENT STORAGE ON A DROPLET ACTUATOR - A method of providing a droplet comprising one or more reagents, the method comprising, depositing a first aqueous droplet comprising the one or more reagents on a surface; drying the droplet to yield a dried composition on the surface comprising the one or more reagents; covering the dried composition with oil; and causing a second aqueous droplet in the oil to contact the dried composition and thereby resuspend one or more reagents. | 05-22-2014 |
20140141410 | FELINE LEUKEMIA VIRUS TRANS-MEMBRANE PROTEIN P15E FOR DIAGNOSIS OF FELV INFECTION - The invention provides for a method for the detection of FeLV infection in a patient, wherein a sample obtained from the patient is contacted in-vitro with a recombinant transmembrane p15E protein in a p15 (E) antibody binding step. | 05-22-2014 |
20140141411 | Methods for Collection, Storage and Transportation of Biological Specimens - The present invention provides methods for collecting, storing or transporting liquid suspension of biological specimens containing analytes of interest in a dry state. The dried biological specimens containing analytes of interest are reconstituted and released for subsequent analysis by compressing or centrifuging the matrix. Also provided are method of using kits for collecting, storing, transporting and recovering biological specimens containing analytes of interest. | 05-22-2014 |
20140141412 | KIT FOR DETECTING MICROORGANISMS - The present invention provides a method for detecting microorganisms comprising steps of PCR, magbead, complex forming, blocking and washing and reporting, in which microorganisms, particular to HBV, can be easily detected via a time-saving and user-friendly process, with high sensitivity and stability. Furthermore, according to the method for detecting microorganisms, a kit for detecting microorganisms is also provided in the present invention, so that the detection of HBV can be achieved conveniently and easily. | 05-22-2014 |
20140147833 | BASE RECOGNITION BASED ON THE CONFORMATION CHANGE OF A MOTOR MOLECULE - A mechanism is provided for base recognition in a nanopore detection system. A complex including a long chain polynucleotide and a motor molecule is formed. The complex is localized in a nanopore of the nanopore detection system. A conformation change of the motor molecule is detected while localized in the nanopore by an ionic current having an amplitude and duration time. The detected conformation change includes the motor molecule forming a base pair by incorporating a single base of the long chain polynucleotide and by synthesizing a complementary base of the single base. An identity of the single base of the long change polynucleotide is determined from the amplitude and the duration time of the conformation change of the motor molecule for the base pair. | 05-29-2014 |
20140147834 | KIT FOR DETECTING BOVINE LEUKEMIA VIRUS(BLV), AND USE THEREOF - A kit for detecting Bovine leukemia virus (BLV) according to the present invention includes: a first PCR primer being oligonucleotide including successive 20 bases or more in the base sequence denoted by SEQ ID NO: 1, the first PCR primer being oligonucleotide having 50 bases or less; and second PCR primer being including successive 20 bases or more in the base sequence denoted by SEQ ID NO: 2, the second PCR primer being oligonucleotide having 50 bases or less is included. | 05-29-2014 |
20140147835 | BASE RECOGNITION BASED ON THE CONFORMATION CHANGE OF A MOTOR MOLECULE - A mechanism is provided for base recognition in a nanopore detection system. A complex including a long chain polynucleotide and a motor molecule is formed. The complex is localized in a nanopore of the nanopore detection system. A conformation change of the motor molecule is detected while localized in the nanopore by an ionic current having an amplitude and duration time. The detected conformation change includes the motor molecule forming a base pair by incorporating a single base of the long chain polynucleotide and by synthesizing a complementary base of the single base. An identity of the single base of the long change polynucleotide is determined from the amplitude and the duration time of the conformation change of the motor molecule for the base pair. | 05-29-2014 |
20140154667 | SOLID MATRIX FOR ONE STEP NUCLEIC ACID AMPLIFICATION - The present invention to relates to methods and kits which can be used to amplify nucleic acids with the advantage of decreasing user time and possible contamination. The PCR reagents are bound to a solid matrix for easy processing and amplification of DNA samples. | 06-05-2014 |
20140154668 | Structures for Enhancement of Local Electric Field, Light Absorption, Light Radiation, Material Detection and Methods for Making and Using of the Same. - This disclosure provides, among other things, a nanosensor comprising a substrate and one or a plurality of pillars extending from a surface of the substrate, where the pillars comprise a metallic dot structure, a metal disc, and a metallic back plane. The nanosensor comprises a molecular adhesion layer that covers at least a part of the metallic dot structure, the metal disc, and/or the metallic back plane and a capture agent bound to the molecular adhesion layer. The nanosensor amplifies a light signal from an analyte, when the analyte is specifically bound to the capture agent. | 06-05-2014 |
20140154669 | Control Nucleic Acids for Multiple Parameters - Methods are provided for the amplification of at least a first and a second target nucleic acid that may be present in at least one fluid sample using an internal control nucleic acid for qualitative and/or quantitative purposes. | 06-05-2014 |
20140154670 | VALIDATION TECHNIQUES FOR FLUID DELIVERY SYSTEMS - A fluid delivery system may include a container that houses a medical fluid, a fluid pressurizing unit, and a fluid transfer set that transfers the medical fluid from the container to the fluid pressurizing unit. To validate the integrity and sterility of the fluid delivery system, the system may undergo testing protocols to evaluate the susceptibility of the system to pathogenic ingress, chemical degradation, and/or fluid cross-contamination between patient fluid delivery procedures. The testing protocols may help ensure that delivery system components used during multiple different fluid delivery procedures perform as well as if the components were replaced after each fluid delivery procedure. | 06-05-2014 |
20140154671 | VALIDATION TECHNIQUES FOR FLUID DELIVERY SYSTEMS - A fluid delivery system may include a container that houses a medical fluid, a fluid pressurizing unit, and a fluid transfer set that transfers the medical fluid from the container to the fluid pressurizing unit. To validate the integrity and sterility of the fluid delivery system, the system may undergo testing protocols to evaluate the susceptibility of the system to pathogenic ingress, chemical degradation, and/or fluid cross-contamination between patient fluid delivery procedures. The testing protocols may help ensure that delivery system components used during multiple different fluid delivery procedures perform as well as if the components were replaced after each fluid delivery procedure. | 06-05-2014 |
20140162244 | ISOTHERMAL NUCLEIC ACID AMPLIFICATION REACTOR WITH INTEGRATED SOLID STATE MEMBRANE - Provided are devices adapted to isolate, amplify, and detect nucleic acids that may be present in a biological sample. The devices can, in some embodiments, isolate, amplify, and detect nucleic acid in a single chamber. In other embodiments, the devices are adapted to isolate and amplify nucleic acids in a reaction chamber, after which the nucleic acids may be communicated to a pervious medium—such as a lateral flow strip—where the user may label and detect the nucleic acids. | 06-12-2014 |
20140162245 | NEW METHOD FOR ENZYME-MEDIATED SIGNAL AMPLIFICATION - The present invention relates to methods and compounds for enzyme-mediated amplification of target-associated signals for visualization of biological or chemical targets in samples, wherein the targets are immobilized, in particular the invention relates to the oxidoreductase-mediated signal amplification for visualization of targets in samples comprising cells. The methods of the invention are particular useful for qualitative and quantitative evaluation of biological markers relating to medical diagnostic and therapy. | 06-12-2014 |
20140162246 | FIXATIVE SOLUTION, FOR FIXATION AND PRESERVATION OF BIOLOGICAL SAMPLES - A fixative solution, suitable for fixation of biological materials such as human or veterinary biological tissues, cells, organs and secretions as well as bacteria, viruses, yeasts, parasites and biotech products such as embalming articles. The fixative solution is not a dangerous product according to European standards, and it contains low concentration of aldehydes or heavy metals, rendering a product that cannot be considered dangerous. Even so, biological samples can be optimally preserved and fixed with this solution. | 06-12-2014 |
20140170637 | Methods for Diagnosing Pervasive Development Disorders, Dysautonomia and Other Neurological Conditions - Methods for aiding in the diagnosis of disorders including, but not limited to, PDDs (Pervasive Development Disorders), Dysautonomic disorders, Parkinson's disease and SIDS (Sudden Infant Death Syndrome). In one aspect, a diagnosis method comprises analyzing a stool sample of an individual for the presence of a biological marker (or marker compound) comprising one or more pathogens, which provides an indication of whether the individual has, or can develop, a disorder including, but not limited to, a PDD, Dysautonomia, Parkinsons disease and SIDS. Preferably, the presence of one or more pathogens is determined using a stool immunoassay to determine the presence of antigens in a stool sample, wherein such antigens are associated with one or more pathogens including, but not limited to, | 06-19-2014 |
20140170638 | HAV detection - Methods for detecting HAV in a biological sample are provided, comprising amplifying a target nucleic acid comprising the sequence of HAV in a reaction mixture. The reaction mixture comprises a biological sample which may contain the target nucleic acid and set of oligonucleotides. The invention also provides kits for the detection of HAV. | 06-19-2014 |
20140170639 | TARGET DETECTION - The invention generally relates to methods for indicating whether an assay for isolating targets is properly isolating and detecting targets. Methods of the invention involve obtaining a sample suspected of a containing target, introducing a detectable marker into the sample, conducting an assay using magnet particles to isolate the detectable marker and the target if it is present in the sample, determining the presence or absence of the target; and confirming that the assay functioned properly by determining presence or absence of the detectable marker. | 06-19-2014 |
20140170640 | METHODS FOR UNIVERSAL TARGET CAPTURE - The invention generally relates to methods for universal target capture. | 06-19-2014 |
20140170641 | SAMPLE ENTRY - The invention generally relates to methods and devices for transferring a sample into a cartridge for processing. Methods of the invention include providing a vessel containing a sample, coupling the sample to a cartridge configured to process the sample at an interface, in which the interface is configured to provide communication between the vessel and the cartridge, introducing a fluid, capture particles, or both from the cartridge into the vessel, and transferring the sample, fluid, and capture particles from the vessel and into the cartridge for processing. | 06-19-2014 |
20140170642 | Methods and Systems for Detecting an Analyte in a Sample - The present disclosure provides methods for the detection of one or more analytes in a sample. Aspects of the methods include flowing a sample (e.g., a biological sample, such as blood) through a channel comprising an analyte specific capture domain stably associated with a surface thereof, wherein the analyte specific capture domain comprises particles displaying a specific binding member for an analyte; and imaging the analyte specific capture domain to detect whether the analyte is present in the sample. Also provided are systems, devices, and kits that may be used in practicing the subject methods. Methods and compositions as described herein find use in a variety of different applications, including diagnostic applications. | 06-19-2014 |
20140178854 | ANTISERA ASSAYS FOR MLV RELATED VIRUSES IN HUMANS AND OTHER MAMMALS - The disclosure provide cell lines and methods for the production of vectors and viral particles useful in diagnostics and therapeutics. | 06-26-2014 |
20140178855 | Murine Astroviruses - Novel murine astroviruses, and methods of detecting the viruses are disclosed. Also disclosed are uses of the viruses and infected animals as model systems for discovery and development of vaccines and therapies for diseases caused by or associated with astrovirus infection, including human astrovirus-based diseases. | 06-26-2014 |
20140178856 | POLYNUCLEOTIDE DERIVED FROM NOVEL HEPATITIS C VIRUS STRAIN AND USE THEREOF - A polynucleotide encoding the amino acid shown in SEQ ID NO:2 or SEQ ID NO: 5, or encoding an amino acid sequence having not less than 98% identity thereto; preferably a polynucleotide comprising replacement of the amino acid corresponding to glutamic acid at position 1202 of SEQ ID NO:2 (position 177 of SEQ ID NO:5) with glycine, replacement of the amino acid corresponding to glutamic acid at position 1056 (position 31 of SEQ ID NO:5) with valine, and replacement of the amino acid corresponding to alanine at position 2199 (position 1174 of SEQ ID NO:5) with threonine. | 06-26-2014 |
20140178857 | Method - The invention provides a method of detecting the presence of anti-MHC antibodies in a sample comprising contacting said sample with one or more recombinant MHC molecules or functionally equivalent variants, derivatives or fragments thereof and detecting the binding or absence of binding of antibodies to said recombinant MHC molecules. This method allows the detection and/or identification of one or more specific MHC particularly HLA allele antibodies. | 06-26-2014 |
20140178858 | AUTOMATED HIGH VOLUME SLIDE PROCESSING SYSTEM - An automated system is provided for performing slide processing operations on slides bearing biological samples. In one embodiment, the disclosed system includes a slide tray holding a plurality of slides in a substantially horizontal position and a workstation that receives the slide tray. In a particular embodiment, a workstation delivers a reagent to slide surfaces without substantial transfer of reagent (and reagent borne contaminants such as dislodged cells) from one slide to another. A method for automated processing of slides also is provided. | 06-26-2014 |
20140178859 | Integrated Assay that Combines Flow-Cytometry and Multiplexed HPV Genotype Identification - A two part assay is disclosed that enables collection of both protein biomarker phenotype and specific HPV genotype data from within a clinically derived population of cervical epithelial cells. Presence of multiple transformation-associated protein biomarkers acts as a gating criterion for cell sorting, followed by application of a PCR protocol sensitive enough to detect and identify individual HPV types from within the cells captured during sorting. The workflow has been optimized to work with cells conventionally fixed in PreservCyt (Cytyc), and it can be performed on residual cells remaining in a stored sample after a Pap test has been performed. | 06-26-2014 |
20140178860 | High Resolution Melt Genotyping of IBV, CSFV and NDV - The present invention relates to methods of differentiating and characterizing IBV, CSFV and NDV strains, and identifying new strains using high resolution melt technology. The present invention also provides primers and kits for use with such methods. | 06-26-2014 |
20140178861 | WAVEGUIDE-BASED DETECTION SYSTEM WITH SCANNING LIGHT SOURCE - The invention provides methods and devices for generating optical pulses in one or more waveguides using a spatially scanning light source. A detection system, methods of use thereof and kits for detecting a biologically active analyte molecule are also provided. The system includes a scanning light source, a substrate comprising a plurality of waveguides and a plurality of optical sensing sites in optical communication with one or more waveguide of the substrate, a detector that is coupled to and in optical communication with the substrate, and means for spatially translating a light beam emitted from said scanning light source such that the light beam is coupled to and in optical communication with the waveguides of the substrate at some point along its scanning path. The use of a scanning light source allows the coupling of light into the waveguides of the substrate in a simple and cost-effective manner. | 06-26-2014 |
20140186819 | Liquid to Liquid Biological Particle Concentrator - A rapid one-pass liquid filtration system efficiently concentrates biological particles that are suspended in liquid from a dilute feed suspension. A sample concentrate or retentate suspension is retained while eliminating the separated fluid in a separate flow stream. Suspended biological particles include such materials as proteins/toxins, viruses, DNA, and/or bacteria in the size range of approximately 0.001 micron to 20 microns diameter. Concentration of these particles is advantageous for detection of target particles in a dilute suspension, because concentrating them into a small volume makes them easier to detect. Additional concentration stages may be added in “cascade” fashion, in order to concentrate particles below the size cut of each preceding stage remaining in the separated fluid in a concentrated sample suspension. This process can also be used to create a “band-pass” concentration for concentration of a particular target size particle within a narrow range. | 07-03-2014 |
20140186820 | MICRO-FLUIDIC DEVICE FOR THE ANALYSIS OF A FLUID SAMPLE - Use of a programmable device with capacitive touch screen for the analysis of a fluid sample as interface between a micro-fluidic device, this micro-fluidic device comprising at least one electrode and at least one micro-channel comprising an input for the introduction of the sample, and the processor of said programmable device with a capacitive touch screen. | 07-03-2014 |
20140186821 | Noninterfering Multipurpose Compositions for Collecting, Transporting and Storing Biological Samples - The invention is directed to compositions and methods for collecting, transporting, and storing microorganisms obtained from samples of biological, clinical, forensic, and environmental origin. Compositions preserve the viability of the collected organisms, permit long-term storage, and are compatible with subsequent manipulation including propagation and culture of collected microorganisms, or isolation, purification, detection, and characterization of proteins, nucleic acids and macromolecules. When the compositions containing microorganisms and polynucleotides therein are further processed, such as by nucleic acid testing, there is an increased ability to detect, isolate, purify and/or characterize select microbes and their components, when compared to conventional microbial transport media that contain interfering substance(s). In particular formulations, the compositions allow biological samples to be collected, transported, and even stored for extended periods, and are compatible with nucleic acid extraction, identification, quantitation, PCR amplification, and genomic analysis methodologies. | 07-03-2014 |
20140186822 | METHODS FOR THE DETECTION OF BIOLOGICALLY RELEVANT MOLECULES AND THEIR INTERACTION CHARACTERISTICS - Methods for the detection of biologically relevant molecules that comprise concentrating such molecules into microscopic holes in a sheet of chemically inert material, restricting the openings, and measuring the electric current through the holes or the fluorescence near the hole openings. The electric current or fluorescence will change as the molecules diffuse out of the holes, providing a measure of the diffusion rate and thereby detecting the presence and characteristics of the molecules. For molecules that interact, the diffusion rate will be slower than for molecules that do not interact, yielding a determination of the molecular interaction. Capping the population of holes and inserting into a mass spectrometer allows identification of the molecules. | 07-03-2014 |
20140193802 | IDENTIFICATION OF AN ALTERED THERAPEUTIC SUSCEPTIBILITY TO ANTI-HCMV COMPOUNDS AND OF A RESISTANCE AGAINST ANTI-HCMV COMPOUNDS - The present invention relates to a method for the detection of an altered therapeutic response of a subject infected by HCMV to a treatment with a 3,4 dihydroquinazoline or N-{3-[({4-[5-(6-aminopyridin-2-yl)-1,2,4-oxadiazol-3-yl]phenyl}sulfonyl)amino]-5-fluorophenyl}-1-cyanocyclopropanecarboxamide, a method for the detection of a drug resistance of a HCMV to a 3,4-dihydroquinazoline or N-{3-[({4-[5-(6-aminopyridin-2-yl)-1,2,4-oxadiazol-3-yl]phenyl}sulfonyl)amino]-5-fluorophenyl}-1-cyanocyclopropanecarboxamide, and to a method for the detection of a mutation of a HCMV resulting in a drug resistance to a 3,4-dihydroquinazoline or N-{3-[({4-[5-(6-aminopyridin-2-yl)-1,2,4-oxadiazol-3-yl]phenyl}sulfonyl)amino]-5-fluorophenyl}-1-cyanocyclopropanecarboxamide. | 07-10-2014 |
20140193803 | METHODS, COMPOSITIONS, AND KITS FOR DETERMINING HEPATITUS A VIRUS - The present invention relates to compositions, methods, and kits for determining the presence or absence of HAV in a sample. | 07-10-2014 |
20140193804 | Biological Specimen Collection and Transport System and Method of Use - Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. The disclosed compositions safely facilitate rapid sample collection, and provide extended storage and transport of the samples at ambient or elevated temperature without contamination of the sample or degradation of the nucleic acids contained therein. This process particularly facilitates the collection of specimens from remote locations, and under conditions previously considered hostile for presenting the integrity of nucleic acids released from lysed biological samples without the need of refrigeration or freezing prior to molecular analysis. | 07-10-2014 |
20140193805 | HPV E6, E7 MRNA Assay and Methods of Use Thereof - Provided is an HPV E6, E7 mRNA assay, referenced herein as the “In Cell HPV Assay,” that is capable of sensitive and specific detection of normal cervical cells undergoing malignant transformation as well as abnormal cervical cells with pre-malignant or malignant lesions. The In Cell HPV Assay identifies HPV E6, E7 mRNA via in situ hybridization with oligonucleotides specific for HPV E6, E7 mRNA and quantitates the HPV E6, E7 mRNA via flow cytometry. The In Cell HPV Assay can be carried out in less than three hours directly from liquid-based cervical (“LBC”) cytology specimens. The In Cell HPV Assay provides an efficient and highly sensitive alternative to the Pap smear for determining abnormal cervical cytology. | 07-10-2014 |
20140193806 | REAL-TIME DETECTION OF INFLUENZA VIRUS - The present invention provides system and methods for detecting an analyte indicative of an influenza viral infection in a sample of bodily fluid. The present invention also provides for systems and method for detection a plurality of analytes, at least two of which are indicative of an influenza viral infection in a sample of bodily fluid. | 07-10-2014 |
20140199682 | INFLUENZA NEUTRALIZING AGENTS - The present invention concerns methods and means for identifying, producing, and engineering neutralizing agents against influenza A viruses, and to the neutralizing agents produced. In particular, the invention concerns neutralizing agents against various influenza A virus subtypes, and methods and means for making such agents. | 07-17-2014 |
20140199683 | INFLUENZA B VIRUSES HAVING ALTERATIONS IN THE HEMAGLUTININ POLYPEPTIDE - The present invention encompasses methods of producing influenza B viruses in cell culture. The influenza B viruses may have desirable characteristics, such as enhanced replication in eggs and may be used, for example, in vaccines and in methods of treatment to protect against influenza B virus infection. | 07-17-2014 |
20140199684 | METHODS FOR AMPLIFYING HEPATITIS C VIRUS NUCLEIC ACIDS - A method of amplifying an HCV nucleic acid in an HCV infected sample comprises amplifying a segment of a DNA template that is complementary to a genome of HCV RNA from the sample by a two-stage PCR, wherein a first stage PCR employs a first outer primer and a second outer primer, and a second stage PCR employs a first inner primer and a second inner primer. The nucleotide sequence of the first outer primer comprises a nucleotide sequence as set forth in SEQ ID NO: 2; or SEQ ID NO:9, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 9. The nucleotide sequence of the second outer primer comprises a nucleotide sequence set forth in SEQ ID NO: 3 or 4; or a nucleotide sequence as set forth in SEQ ID NO: 10 or 11, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 10 and 11. The nucleotide sequence of the first inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 5; or SEQ ID NO:12, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 12. The nucleotide sequence of the second inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 6 or 7; or a nucleotide sequence as set forth in SEQ ID NO: 13 or 14, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 13 and 14. | 07-17-2014 |
20140199685 | DEVICES FOR THE DETECTION OF MULTIPLE ANALYTES IN A SAMPLE - The present invention relates generally to an assay for detecting and differentiating multiple analytes, if present, in a single fluid sample, including devices and methods therefore. | 07-17-2014 |
20140199686 | COMPOSITIONS AND METHODS FOR RECOVERY OF NUCLEIC ACIDS OR PROTEINS FROM TISSUE SAMPLES FIXED IN CYTOLOGY MEDIA - The present invention provides compositions and methods for improving nucleic acid or protein recovery from fixed biological samples. | 07-17-2014 |
20140199687 | Nucleic Acid Sequences That Can Be Used as Primers and Probes in the Amplification and Detection of All Subtypes of HIV-1 - The present invention is related to nucleic acid sequences that can be used in the field of virus diagnostics, more specifically the diagnosis of infections with the AIDS causing Human Immuno-deficiency Virus (HIV). With the present invention nucleotide sequences are provided that can be used as primers and probes in the amplification and detection of HIV-1 nucleic acid. The oligonucleotide sequences provided with the present invention are located in the LTR part of the HIV viral genome. It has been found that, by using the sequences of the present invention in methods for the amplification and detection of nucleic acid a sensitive and specific detection of HIV-1 can be obtained. The benefit of the sequences of the present invention primarily resides in the fact that, with the aid of primers and probes comprising the sequences according to the invention the nucleic acid of all presently known subtypes of HIV-1 can be detected with high accuracy and sensitivity. So far no primer pairs or hybridization probes have been developed that would allow the detection of such a broad range of HIV-1 variants. The oligonucleotide sequences according to the present invention are especially useful in methods for the amplification of nucleic acid. | 07-17-2014 |
20140199688 | CONDITIONALLLY REPLICATION-COMPETENT ADENOVIRUS - The object of the present invention is to provide a novel conditionally replicating adenovirus and a reagent comprising the same for cancer cell detection or for cancer diagnosis. | 07-17-2014 |
20140205993 | RECOMBINANT AVIAN INFLUENZA VACCINE AND USES THEREOF - The present invention encompasses influenza vaccines, in particular avian influenza vaccines. The vaccine may be a subunit vaccine based on the hemagglutinin of influenza. The hemagglutinin may be expressed in plants including duckweed. The invention also encompasses recombinant vectors encoding and expressing influenza antigens, epitopes or immunogens which can be used to protect animals against influenza. It encompasses also a vaccination regimen compatible with the DIVA strategy, including a prime-boost scheme using vector and subunit vaccines. | 07-24-2014 |
20140205994 | Methods, Devices, Kits and Compositions for Detecting Roundworm, Whipworm, and Hookworm - Methods, devices, kits and compositions for detecting the presence or absence of one or more helminthic coproantigens in a sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm, whipworm and/or hookworm in a fecal sample from a mammal and may also be able to distinguish between one or more helminth infections. Confirmation of the presence or absence of roundworm, whipworm and/or hookworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated. | 07-24-2014 |
20140205995 | MOLECULAR CONJUGATE - A method is disclosed for making a conjugate of two molecules using a hydrazide thiol linker. In a particular working embodiment, an Fc-specific antibody-enzyme conjugate is made using the method and demonstrated to provide exceptional staining sensitivity and specificity in immunohistochemical and in situ hybridization assays. | 07-24-2014 |
20140205996 | DISSOLVABLE FILMS AND METHODS INCLUDING THE SAME - A method includes providing a container, introducing a substance into the container, and introducing a readily dissolvable film into the container such that the dissolvable film overlies the substance within the container. An alternative method includes providing a container, providing a readily dissolvable film, the film comprising a substance carried by the film, and introducing the film into the container. | 07-24-2014 |
20140205997 | FLUIDIC CONNECTORS AND MICROFLUIDIC SYSTEMS - Fluidic connectors, methods, and devices for performing analyses (e.g., immunoassays) in microfluidic systems are provided. In some embodiments, a fluidic connector having a fluid path is used to connect two independent channels formed in a substrate so as to allow fluid communication between the two independent channels. One or both of the independent channels may be pre-filled with reagents (e.g., antibody solutions, washing buffers and amplification reagents), which can be used to perform the analysis. These reagents may be stored in the channels of the substrate for long periods amounts of time (e.g., 1 year) prior to use. | 07-24-2014 |
20140220554 | PROCESSES AND COMPOSITIONS FOR DETECTING HUMAN PAPILLOMA VIRUS TYPES - Provided herein are compositions and processes that allow for sensitive detection of up to fifteen individual HPV sequences or types in a single, multiplexed test. High risk types that can be detected are HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68, and 73. Processes and compositions described herein are based in part on the presence or absence of HPV nucleic acid, including HPV DNA and RNA. | 08-07-2014 |
20140220555 | IN VITRO MICROPHYSIOLOGICAL SYSTEM FOR HIGH THROUGHPUT 3D TISSUE ORGANIZATION AND BIOLOGICAL FUNCTION - Techniques for generating microtissues, including a micro-fabricated platform including at least one micro-well including a plurality of micro-cantilevers coupled thereto and surrounded by a plurality of ridges, each micro-cantilever including a cap at a terminal end thereof. The platform can be immersed in a suspension of cells. The suspension of cells can be driven into at least one micro-well, and the ridges can be de-wetted to remove excess suspension and isolate the suspension of cells in each micro-well. The cells can be driven in the suspension of each micro-well toward a top surface of the suspension, which can be polymerized to form a matrix. The cells can be cultivated to spontaneously compact the matrix such that the micro-cantilevers anchor and constrain the contracting matrix to form a band of microtissue that spans across the micro-cantilevers. | 08-07-2014 |
20140234830 | DIRECT AMPLIFICATION AND DETECTION OF VIRAL AND BACTERIAL PATHOGENS - Provided are methods for identifying the presence or absence of a target nucleic acid from a microorganism using direct amplification without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids. Further provided are reagent mixtures that allow for direct amplification of a sample, without the step of nucleic acid extraction. | 08-21-2014 |
20140234831 | Packaging or Other Materials Comprising a Biosensor and Methods of Their Use - Provided herein is a composition for detection of a microbial product in a sample comprising a polydiacetylene (PDA) and a packaging polymer, wherein the sample contacts the PDA and a change of PDA color indicates detection. Also provided herein are methods of making a packaging material comprising a PDA and methods of using the composition for detection of a microbial product in a sample. | 08-21-2014 |
20140242571 | OPTICAL ELEMENT, ANALYSIS EQUIPMENT, ANALYSIS METHOD AND ELECTRONIC APPARATUS - An optical element includes a metal layer in which a first direction is a thickness direction; metallic particles provided to be spaced from the metal layer in the first direction; and a light transmitting layer that separates the metal layer from the metallic particles, in which the size T of the metallic particles in the first direction satisfies a relationship of 3 nm≦T≦14 nm, and the size D of the metallic particles in a second direction orthogonal to the first direction satisfies a relationship of 30 nm≦D<50 nm. | 08-28-2014 |
20140242572 | CHROMATOGRAPHY METHOD, CHROMATOGRAPHY KIT, AND METHOD OF PRODUCING AN INSOLUBLE CARRIER FOR CHROMATOGRAPHY - A chromatography method including a step of developing a complex of a specimen and a labeled substance modified by a first bondable substance which can be bonded to the specimen on an insoluble carrier, a step of capturing the complex of the specimen and the labeled substance at a reactive site on the insoluble carrier, a step of washing the reactive site on the insoluble carrier using a washing liquid, a step of detecting the specimen which is captured at the reactive site after a signal of the labeled substance is amplified by an amplification liquid, in which the reactive site on the insoluble carrier includes (i) a second bondable substance which can be bonded to the specimen or a substance having affinity to the first bondable substance which can be bonded to the specimen, and (ii) polyethylene glycol having the molecular weight from 1,500 to 10,000. | 08-28-2014 |
20140242573 | OPTICAL ELEMENT, ANALYSIS DEVICE, ANALYSIS METHOD AND ELECTRONIC APPARATUS - An optical element includes a metal layer having a thickness in a first direction; metallic particles spaced apart from the metal layer in the first direction; and a light transmitting layer separating the metal layer from the metallic particles. The metallic particles are disposed in a lattice shape in a second direction orthogonal to the first direction and in a third direction orthogonal to the first direction and the second direction. A distance between adjacent metal particles in the second and third directions is S, and 6 nm08-28-2014 | |
20140248602 | TORQUE TENO VIRUS DIAGNOSTICS - The present invention relates to methods for the detection of the presence of swine Torque Teno virus in a sample, for the detection of replication of swine Torque Teno virus in a sample, to Torque Teno virus (RT)-PCR primers and probes, and to diagnostic test kits for the detection of the presence and replication of swine Torque Teno virus in a sample. | 09-04-2014 |
20140248603 | QUANTIFICATION OF VACCINE COMPOSITIONS - The invention provides methods and mass-labeled peptides for use in said methods for quantifying the presence of a one or more viral proteins in a sample of a preparation containing agents which bind to said viral protein, using mass-spectroscopic analyses of the sample and standards containing known amounts of labeled and unlabeled signature peptides, in particular wherein said viral proteins are antigens in a vaccine for porcine circovirus. | 09-04-2014 |
20140248604 | METHOD FOR DETECTING HIV-1 CO-RECEPTOR TROPISM - A method for determining HIV-1 co-receptor tropism in an HIV-infected patient includes preparing an HIV-1 envelope protein coding sequence from a sample, introducing the HIV-1 envelope protein coding sequence into a first expression construct using yeast homologous recombination, and using the first expression construct in a cell to cell fusion assay to determine HIV-1 co-receptor tropism. | 09-04-2014 |
20140248605 | BACTERIAL CHALLENGE MODEL IN CATTLE USING A TRANS- AND INTRA-DERMAL ROUTE TO INFECT PERIPHERAL LYMPH NODES - The present invention includes a method of observing and evaluating bacterial infections within the lymph nodes of animals presented for harvest comprising: inoculating at one or more sites of an animal a known amount of a pathogen, wherein the one or more inoculation sites comprise lymph node drainage areas, and at one or more time points obtaining one or more lymph node biopsies to determine the extent of the pathogen in the lymph nodes. | 09-04-2014 |
20140248606 | CELL- OR VIRUS SIMULATING MEANS COMPRISING ENCAPSULATED MARKER MOLECULES - The present invention refers to a method, a composition and a kit for isolating biomolecules from any biological sample material containing cells, virus(es), microorganism(s) or a combination thereof comprising a cell- or virus-simulating means, wherein said cell- or virus-simulating means comprises at least one type of marker molecule(s), incorporated in at least one type of a layer, capsule, bead, sphere or particle, which is not a biological cell or provided on a substrate covered by a coating. | 09-04-2014 |
20140248607 | BRUCELLA PHAGE POLYNUCLEOTIDES AND USES THEREOF - An isolated polynucleotide is disclosed which comprises a nucleic acid sequence of a | 09-04-2014 |
20140255911 | SAMPLE DETECTION APPARATUS AND DETECTION METHOD - According to one embodiment, a sample detection apparatus including an insulating partition to divide a first and a second region, a pore formed in the partition, a first electrode arranged in the first region, a second electrode arranged in the second region, a power source configured to apply electrical current between the first and second electrode in a state in which a reagent containing a capture substance to be bound to a target and a tag particle bound to the capture substance is introduced into the first region together with a sample, and an electrolyte solution is introduced into the second region, a measurement unit configured to observe a change in a conductive state, and a detection unit to detect presence/absence of the target in the sample based on an observation result. | 09-11-2014 |
20140255912 | Sample Collection and Analysis - Management of the health status of an animal colony using a plurality of blood collection cards and the analysis of dried blood from members of the colony that has been collected on the cards. Members of the colony may be removed from the colony as a result of the analysis. | 09-11-2014 |
20140255913 | ANALYSIS DEVICE, ANALYSIS METHOD, OPTICAL ELEMENT AND ELECTRONIC APPARATUS FOR ANALYSIS DEVICE AND ANALYSIS METHOD, AND METHOD OF DESIGNING OPTICAL ELEMENT - An analysis device includes an optical element which includes a metal layer, a light transmitting layer provided on the metal layer to transmit light, and a plurality of metal particles arranged at a first interval P1 in a first direction and arranged at a second interval P2 in a second direction intersecting the first direction on the light transmitting layer, P109-11-2014 | |
20140255914 | COMPOSITIONS AND METHOD FOR DETECTING HUMAN PARVOVIRUS NUCLEIC ACID AND FOR DETECTING HEPATITIS A VIRUS NUCLEIC ACIDS IN SINGLE-PLEX OR MULTIPLEX ASSAYS - Nucleic acid oligomers specific for human parvovirus genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus genotypes 1, 2 and 3 nucleic acid in biological specimens is disclosed. Compositions for amplifying and detecting the presence of human parvovirus genotypes 1, 2 and 3 genomic DNA in human biological specimens are disclosed. | 09-11-2014 |
20140255915 | ASSAY FOR DETECTION OF JC VIRUS DNA - In one aspect, the disclosure provides methods for isolating nucleic acid from a Cerebrospinal Fluid (CSF) sample. In one aspect, the disclosure provides methods for determining the amount of JC virus DNA in a sample. | 09-11-2014 |
20140255916 | SEROLOGY ASSAYS - The invention provides methods and kits for measuring the ability of a test sample to inhibit the binding of a receptor expressed by a pathogen to a host cell ligand of the pathogen. | 09-11-2014 |
20140272928 | SYSTEMS AND METHODS FOR DETECTION OF CELLS USING ENGINEERED TRANSDUCTION PARTICLES - Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity. | 09-18-2014 |
20140272929 | METHODS AND SYSTEMS FOR IMPROVED CAVITATION EFFICIENCY AND DENSITY, CANCER CELL DESTRUCTION, AND/OR CAUSING A TARGET OBJECT TO BE A CAVITATION NUCLEUS - A method of causing an object to act as a cavitation nucleus comprising the steps of providing a sound energy source capable of generating a sound energy over a plurality of frequency ranges, providing a target object contained in a liquid, coupling the sound energy source to the liquid, determining a specific frequency range, within the frequency ranges, that resonates the target object, and exposing the target object to sound energy at the specific frequency range to cause the target object to act as a cavitation nucleus. | 09-18-2014 |
20140272930 | Compositions And Methods For Determining Resistance To Inhibitors Of Virus Entry Using Recombinant Virus Assays - The invention provides a method for determining whether a human immunodeficiency virus is resistance to a viral entry inhibitor. The methods are particularly useful for determining resistance to inhibitors that act by a non-competitive mechanism. In certain aspects, the methods comprise determining whether an HIV population is resistant to an HIV entry inhibitor, comprising determining a log-sigmoid inhibition curve comprising data points for entry of the HIV population in the presence of varying concentrations of the HIV entry inhibitor, wherein if the entry of the HIV population cannot be completely inhibited by the HIV entry inhibitor, the HIV population is resistant to the HIV entry inhibitor. | 09-18-2014 |
20140272931 | HCV CORE LIPID BINDING DOMAIN MONOCLONAL ANTIBODIES - The present invention relates to monoclonal antibodies for the detection of HCV antigen. More specifically, the invention describes antibodies against HCV core antigen lipid binding domain and immunoassay methods, kits and compositions for use in detecting HCV infection. | 09-18-2014 |
20140272932 | HCV NS3 RECOMBINANT ANTIGENS AND MUTANTS THEREOF FOR IMPROVED ANTIBODY DETECTION - The present disclosure relates to polypeptides, including fusions thereof, nucleic acids, vectors, host cells, immunodiagnostic reagents, kits, and immunoassays for use detecting the presence of HCV antibodies. More specifically, the present invention describes specific NS3 antigens that can be used for the detection of anti-HCV antibodies. | 09-18-2014 |
20140272933 | HCV ANTIGEN-ANTIBODY COMBINATION ASSAY AND METHODS AND COMPOSITIONS FOR USE THEREIN - The present invention generally relates to combination immunoassays, reagents and kits for simultaneous detection of HCV antigens and anti-HCV antibodies in a test sample. | 09-18-2014 |
20140272934 | DENDRIMERIC DYE-CONTAINING OLIGONUCLEOTIDE PROBES AND METHODS OF PREPARATION AND USES THEREOF - The invention provides novel oligonucleotide probes that have dendrimeric dyes useful for detecting and analyzing biological samples, and compositions and methods thereof. The dendrimeric dye-containing oligonucleotide probes are useful for high sensitivity fluorescence in situ hybridization (FISH) of nucleic acids such as DNA and RNA. | 09-18-2014 |
20140272935 | MIXING OF FLUIDS IN FLUIDIC SYSTEMS - Fluidic devices and methods associated with mixing of fluids in fluidic devices are provided. In some embodiments, a method may involve the mixing of two or more fluids in a channel segment of a fluidic device. The fluids may be in the form of, for example, at least first, second and third fluid plugs, composed of first, second, and third fluids, respectively. The second fluid may be immiscible with the first and third fluids. In certain embodiments, the fluid plugs may be flowed in series in the channel segment, e.g., in linear order, causing the first and third fluids to mix without the use of active to components such as mixers. The mixing of fluids in a channel segment as described herein may allow for improved performance and simplification in the design and operations of fluidic devices that rely on mixing of fluids. | 09-18-2014 |
20140272936 | ADULTERATION TESTING OF HUMAN MILK - The present invention provides a method for screening human milk for an adulterant, e.g., non-human milk and infant formula, as well as methods of making human milk compositions free of an adulterant, e.g., human milk fortifiers and standardized human milk formulations. | 09-18-2014 |
20140272937 | Low Resource Processor Using Surface Tension Valves for Extracting, Concentrating, and Detecting Whole Cells - Systems and methods are described for isolation, separation and detection of a molecular species using a low resource device for processing of samples. Methods include isolation, separation and detection of whole cells. | 09-18-2014 |
20140272938 | DEVICES, SYSTEMS AND METHODS FOR SAMPLE PREPARATION - Devices, systems and methods including a sonicator for sample preparation are provided. A sonicator may be used to mix, resuspend, aerosolize, disperse, disintegrate, or de-gas a solution. A sonicator may be used to disrupt a cell, such as a pathogen cell in a sample. Sample preparation may include exposing pathogen-identifying material by sonication to detect, identify, or measure pathogens. A sonicator may transfer ultrasonic energy to the sample solution by contacting its tip to an exterior wall of a vessel containing the sample. Multipurpose devices including a sonicator also include further components for additional actions and assays. Devices, and systems comprising such devices, may communicate with a laboratory or other devices in a system for sample assay and analysis. Methods utilizing such devices and systems are provided. The improved sample preparation devices, systems and methods are useful for analyzing samples, e.g. for diagnosing patients suffering from infection by pathogens. | 09-18-2014 |
20140272939 | ASSAY METHODS - The present invention is directed to methods for improving assay specificity and performance in binding assays. | 09-18-2014 |
20140272940 | METHODS FOR DETECTION OF MULTIPLE TARGET NUCLEIC ACIDS - The present disclosure, in some embodiments, relates to compositions and methods for detection of multiple target nucleic acid sequences by a single nucleic acid amplification based assay (e.g. PCR). Compositions comprising two or more primers are described for multiple target nucleic acid detection. Methods for differential detection of microorganisms (including strains/serovars/subtypes thereof) and cell types comprise a single step method to detect a signature of target nucleic acid sequences comprising: two, three or more target nucleic acids that are uniquely present in a microorganism/strain/serovar/subtype and/or cell and absent in other closely related organisms/cells. Embodiments relate to methods of diagnosis of diseases or conditions that can be detected by detecting the presence of two or more nucleic acid target markers. | 09-18-2014 |
20140272941 | ROTATABLE FLUID SAMPLE COLLECTION DEVICE - A sample collection device for a fluid sample includes: a body including a capillary channel having a first end and a second end, wherein the first end is adapted to draw the fluid into the channel by capillary action; an air vent located in the vicinity of the second end and in fluid communication with the capillary channel; a barrier positioned within the capillary channel to prevent flow of the fluid by capillary action thereacross; and features on opposing sides of the body to form an axis of rotation, which is substantially perpendicular to the overall direction of the capillary channel from the first end to the second end. In a preferred embodiment, the sample collection device is adapted to rotate about the axis of rotation within a cartridge having a sample manipulation device to bring the first end into position with the sample manipulation device. | 09-18-2014 |
20140272942 | Methods of Detecting Influenza Virus - Provided herein is a method of detecting the presence of influenza virus in a sample while minimizing false positives due to presence of one or more other pathogens in the sample, the method including measuring the enzymatic activity of neuraminidase (NA) in the sample under one or more differentiating conditions selected from the group consisting of pH, binding to anti-NanA antibody, size exclusion, hemagglutinin (HA) binding, chemical inhibition, and combinations thereof. | 09-18-2014 |
20140272943 | RAPID AND HIGHLY FIELDABLE VIRAL DIAGNOSTIC - The present invention relates to a rapid, highly fieldable, nearly reagentless diagnostic to identify active RNA viral replication in a live, infected cells, and more particularly in leukocytes and tissue samples (including biopsies and nasal swabs) using an array of a plurality of vertically-aligned nanostructures that impale the cells and introduce a DNA reporter construct that is expressed and amplified in the presence of active viral replication. | 09-18-2014 |
20140272944 | ONE-STEP PROCEDURE FOR THE PURIFICATION OF NUCLEIC ACIDS - The present invention is a new and non-obvious method for the improved and simplified purification of nucleic acids. | 09-18-2014 |
20140272945 | METHOD FOR MANUFACTURING MULTI-FUNCTIONAL BIO-MATERIAL CONJUGATE USING TWO KINDS OF PARTICLE, AND MULTI-FUNCTIONAL BIO-MATERIAL CONJUGATE MANUFACTURED BY MEANS OF SAME - Disclosed herein are a method for manufacturing a multi-functional bio-material conjugate used as a biosensor for detecting microorganisms, and the like, and a multi-functional bio-material conjugate manufactured by means of the same. The method for manufacturing a multi-functional bio-material conjugate includes: (a) coating a first nanoparticle having magnetic or fluorescent characteristics with protein; (b) manufacturing a conjugate by adsorbing a second nanoparticle having metallic characteristics onto the first nanoparticle coated with protein; and (c) manufacturing the multi-functional bio-material conjugate by adsorbing a bio-material onto the conjugate. The method for manufacturing a multi-functional bio-material conjugate according to the present invention may prevent precipitation of the nanoparticles, easily immobilize the bio-material, and manufacture a bio-material conjugate having multiple functions, by using two kinds of the particles. In addition, the multi-functional bio-material conjugate manufactured by the present method may be used to detect microorganisms at up to a concentration of 10 | 09-18-2014 |
20140287402 | METHOD FOR SCREENING CELLS - The invention relates to high throughput screening methods for identifying, isolating and retrieving cells secreting antibodies of interest, e.g., having functional activity and/or multi-antigen specificities. It further provides methods for the cloning of said antibodies VH/VL sequences and the generation of recombinant monoclonal antibodies derived thereof having the features of interest. | 09-25-2014 |
20140287403 | NANOSCALE MOTION DETECTOR - Motion detector comprising a flexible support ( | 09-25-2014 |
20140295403 | DETECTION OF BIOLOGICAL MOLECULES BY DIFFERENTIAL PARTITIONING OF ENZYME SUBSTRATES AND PRODUCTS - This invention relates to a method and apparatus for detecting a biological molecule associated with enzyme activity in a sample. The invention is applicable to detecting a microorganism associated with an enzyme in a sample such as water, food, soil, or a biological sample. According to a preferred embodiment of the method of the invention, a sample containing an enzyme of interest or a microorganism associated with the enzyme is combined with a suitable substrate, and a fluorescent product of the enzyme-substrate reaction is selectively detected. The fluorescent product is detected with a partitioning element or optical probe/partitioning element of the invention. In one embodiment the partitioning element provides for partitioning of only the fluorescent product molecule into the probe. The invention also provides an automated system for monitoring for biological contamination of water or other samples. | 10-02-2014 |
20140295404 | SAMPLE FIXATION AND STABILISATION - It has surprisingly been discovered that it is possible to stabilise biomolecules such as RNA, DNA and proteins in biological samples such as cells, tissues, biopsies and blood using deep eutectic solvents (DES). It has also been discovered that DES mixtures can be used to fix and preserve cell morphology in biological samples such as tissue blocks, cancer biopsies and whole blood. This invention describes methods to stabilise and preserve biomolecules, whole cells, tissues, blood and biological samples using DES mixtures. | 10-02-2014 |
20140295405 | CHROMATOGRAPHY METHOD, AND CHROMATOGRAPHY KIT - The chromatography method of the present invention includes (a) developing a complex of a test substance and a labeling substance modified with a first binding substance bindable to the test substance, onto an insoluble carrier, (b) causing the complex to be trapped in a reaction site on the insoluble carrier which contains a second binding substance bindable to the test substance or a substance exhibiting binding properties to the first binding substance, (c) washing the insoluble carrier with a washing solution containing at least one of the potassium iodide, urea, and guanidine after the step (b), (d) washing off the washing solution of the step (c) remaining in the insoluble carrier from the insoluble carrier, and (e) amplifying the labeling substance of the complex trapped in the reaction site. | 10-02-2014 |
20140295406 | Portable Device for Detection of Harmful Substances - A self-contained apparatus and methods for detecting the presence of any specified substance in any medium. A sample of the medium is placed in a capsule, along with a solvent and a sensor configured to test for a target analyte. The solvent comes into contact with the medium in the capsule, and the capsule is agitated to create a dispersion in the solvent of a portion of any target analyte present in the medium. A release mechanism configured to cause conduction of the dispersion to the sensor, so that the sensor produces an indication of presence of the target analyte if the target analyte is present in the medium. The apparatus uses a disposable capsule where the medium in question is placed and the disposable capsule is placed inside a reader and analyzed for presence of the harmful substance. | 10-02-2014 |
20140295407 | BIOSENSOR BASED ON THE INNATE IMMUNE SYSTEM - Described herein are biosensors for early, pre-symptomatic detection of infectious agents and methods for their use. In particular, this disclosure describes biosensors that utilize toll-like receptor (TLR) binding domains to detect pathogen-associated molecular patterns (PAMPs). Also provided herein are methods of detecting and/or capturing a PAMP from a biological sample using the disclosed biosensors. | 10-02-2014 |
20140295408 | COMPOSITIONS, METHODS, AND KITS FOR NUCLEIC ACID HYBRIDIZATION - Polynucleotides having a first polynucleotide segment contiguous with a second polynucleotide segment that is downstream to the first, wherein the sequence of the first polynucleotide segment is complementary to the sequence of a first probe segment of a probe for detection of a target nucleic acid sequence, wherein the sequence of the second polynucleotide segment is complementary to the sequence of a second probe segment of the probe, wherein the second probe segment is downstream to the first probe segment, are provided. The polynucleotides may be employed with dual-labeled probes (DLPs) in assays for the detection of the target nucleic acid sequences. | 10-02-2014 |
20140295409 | METAPNEUMOVIRUS STRAINS AND THEIR USE IN VACCINE FORMULATIONS AND AS VECTORS FOR EXPRESSION OF ANTIGENIC SEQUENCES - The present invention provides an isolated mammalian negative strand RNA virus, | 10-02-2014 |
20140302486 | SYSTEMS AND METHODS FOR DETECTING BIOMARKERS OF INTEREST - A detection probe for detecting single base mutations or alterations in a double stranded target nucleic acid molecule is provided. In some aspects, the detection probe may include a double-stranded probe nucleic acid molecule having a first end and a second end; at least one probe initiation toehold at the first end; at least one substance capable of emitting a detectable signal at the second end; and optionally, at least one dissociation toehold at the second end. The detection probe is designed to hybridize with a double stranded target nucleic acid in a reaction which proceeds at approximate thermodynamic equilibrium (ΔG≈0) when no single base mutations or alterations are present in the double stranded target nucleic acid molecule. The probe may be used in detection systems and methods to identifying the presence or absence of one or more single base mutations or alterations in a double-stranded nucleic acid target molecule. | 10-09-2014 |
20140302487 | Recombinant Phage and Bacterial Detection Methods - Methods of detecting target bacteria are provided. In some embodiments the methods comprise exposing the sample to a phage capable of infecting a set of target bacteria and comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments the target bacteria comprise | 10-09-2014 |
20140302488 | Vaccine Diagnostics - The present invention relates to improved diagnostic methods and kits for differentiating between (a) animals administered a chimeric pestivirus, and (b) animals infected with a wild-type bovine viral diarrhea virus (BVDV) or immunized with a conventional BVDV vaccine. | 10-09-2014 |
20140302489 | IDENTIFICATION OF PROTECTIVE ANTIGENIC DETERMINANTS OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS AND USES THEREOF - The invention relates to a polypeptide of a protective antigenic determinant (PAD polypeptide) of porcine reproductive and respiratory syndrome virus (PRRSV) and nucleic acids encoding a PAD polypeptide. The PAD polypeptide and nucleic acids encoding a PAD polypeptide are useful in the development of antibodies directed to PAD, vaccines effective in providing protection against PRRSV infection, and diagnostic assays detecting the presence of PAD antibodies generated by a PAD-specific vaccine. The invention also discloses methods of generating antibodies to PAD, for vaccinating a pig to provide protection from PRRSV infections, a method of preparing the vaccine, a method of treating PRRSV infections in a pig, and a method of detecting antibodies to PAD of PRRSV. | 10-09-2014 |
20140302490 | METHOD AND DEVICE FOR OPTICAL ANALYSIS OF PARTICLES AT LOW TEMPERATURES - Method and device ( | 10-09-2014 |
20140302491 | Ex Vivo Culture, Proliferation and Expansion of Primary Tissue Organoids - Culture systems and methods for long term culture of mammalian tissues are provided. Tissues include but are not limited to lung alveolar tissue, stomach tissue, pancreas tissue, bladder tissue, liver tissue, and kidney tissue. Cultures are initiated with fragments of mammalian tissue, which are then cultured embedded in a gel substrate that provides an air-liquid interface. Cultured explants of the invention can be continuously grown in culture for a year or more, while maintaining features of the tissue including prolonged tissue expansion with proliferation, multilineage differentiation, and recapitulation of cellular and tissue ultrastructure. | 10-09-2014 |
20140302492 | METHOD OF DETECTING AND/OR QUALIFYING AN ANALYTE IN A BIOLOGICAL SAMPLE - An aptamer-based SERS detection technique that directly monitors an aptamer-analyte capture event by generating spectroscopic information regarding the identity of the analyte that has been bound to the aptamer from a complex biological sample. A reproducible SERS spectrum is measured for an aptamer-analyte complex formed on a metal surface and this spectral information is used directly to identify the specific aptamer-analyte complex and optionally also to quantify the analyte in the sample, thus enabling discrimination between true and false positives in quantitative analyte assays on complex biological samples. In one embodiment the aptamer is attached directly to the metal surface and surrounded by a self-assembled monolayer (SAM) of amphiphilic molecules. In an alternative embodiment the metal surface is coated with a SAM and the aptamer is attached to the amphiphilic molecules of the SAM. | 10-09-2014 |
20140308657 | SERINE PROTEASE INHIBITOR KAZAL ANTIBODIES - This invention describes a relevant etiology of cancer and a novel anti-cancer therapeutic strategy, based on the discovery that a protein named serine protease inhibitor (SPIK/SPINK/PSTI) was up-regulated by hepatitis B and C virus infections consequently suppressing the cell apoptosis. Accordingly, this invention provides an inhibitor of SPIK and/or a technology of suppression of over-expression of SPIK in cells. The inhibitors include: 1) chemical compounds, which can inhibit SPIK transcripts, protein activity, and gene expression, 2) SPIK siRNA (RNAi gene silence or dsRNA of SPIK, 3) DNA anti-sense and anti-SPIK antibody. Further, this invention provides a method of using the inhibitor as an anti-cancer agent to re-instate cancer cell apoptosis (e.g., serine protease dependent cell apoptosis). Also provided is an anti-SPIK antibody specific for an epitope comprising the first nine amino acids of intact SPIK. Further, a diagnostic kit is provided comprising at least one antibody specific for an epitope comprising the first nine amino acids of intact SPIK to diagnose patients exhibiting disease symptoms or at risk for developing a disease, wherein the disease is HBV infection, HCV infection, hepatitis, cancer or hepatic cancer. | 10-16-2014 |
20140308658 | UTILITIES OF STIMULATED WHOLE BLOOD CULTURE SYSTEMS - The invention describes a method for determining how to stimulate, monitor and/or inhibit virus production in whole blood culture. The invention relates to a test kit for performing the method and to the use of a suitable blood sampling system. The system can be used to determine how to activate or target latently HIV-infected cells, for clinical management of HIV treatments and for personalized therapeutic strategies. | 10-16-2014 |
20140308659 | Liquid to Liquid Biological Particle Concentrator - A rapid one-pass liquid filtration system efficiently concentrates biological particles that are suspended in liquid from a dilute feed suspension. A sample concentrate or retentate suspension is retained while eliminating the separated fluid in a separate flow stream. Suspended biological particles include such materials as proteins/toxins, viruses, DNA, and/or bacteria in the size range of approximately 0.001 micron to 20 microns diameter. Concentration of these particles is advantageous for detection of target particles in a dilute suspension, because concentrating them into a small volume makes them easier to detect. Additional concentration stages may be added in “cascade” fashion, in order to concentrate particles below the size cut of each preceding stage remaining in the separated fluid in a concentrated sample suspension. This process can also be used to create a “band-pass” concentration for concentration of a particular target size particle within a narrow range. | 10-16-2014 |
20140308660 | METHOD FOR DISCRIMINATION OF METAPLASIAS FROM NEOPLASTIC OR PRENEOPLASTIC LESIONS - The present invention relates to a method for discrimination of p16.sup.INK4a overexpressing metaplasias from neoplastic or preneoplastic p16.sup.INK4a overexpressing lesions by determination of the level of high risk HPV encoded gene-products such as e.g. HPV E2 and/or HPV E7 molecules in biological samples in the course of cytological testing procedures. The method thus enables for reduction of false positive results in the p16.sup.INK4a based detection of anogenital lesions in cytological testing procedures. | 10-16-2014 |
20140315186 | IN-VITRO DIAGNOSTICS FOR CAPRINE ARTHRITIS-ENCEPHALITIS VIRUS ANTISERA AND OTHER VIRAL INTERFERENCE RELATED AGENTS - Various postulates and methodologies in using Caprine Arthritis Encephalitis Virus and other related agents to diagnose and treat HIV/AIDS as well as other related and/or non-related diseases are declared. | 10-23-2014 |
20140315187 | Pharmaceutical Product and Method of Analysing Light Exposure of a Pharmaceutical Product - A pharmaceutical product includes a container having an exterior surface and an interior chamber, an active ingredient disposed in the interior chamber, the active ingredient having a photosensitive property that changes based on at least cumulative exposure to light having a wavelength within the range between X and Y, and a layer of photosensitive material disposed on or in the container and exposed to environmental conditions contemporaneous with the active ingredient being disposed in the interior chamber. The photosensitive material is reactive to light having a wavelength within the range between X and Y to experience a property change at a threshold of cumulative exposure to light received within the range between X and Y related to the change in the photosensitive property of the active ingredient. A photosensitive device can also be disposed along a path followed by the pharmaceutical product within a facility. | 10-23-2014 |
20140315188 | ASSESSMENT OF PML RISK AND METHODS BASED THEREON - The invention provides a method of assessing the risk of occurrence of progressive multifocal leukoencephalopathy (PML) in a subject as well as a method of stratifying a subject undergoing α | 10-23-2014 |
20140315189 | CO-BINDER ASSISTED ASSAY METHODS - The present invention is directed to methods for reducing cross-reactivity between species employed in multiplexed immunoassays. | 10-23-2014 |
20140322701 | MINIATURIZED, AUTOMATED IN-VITRO TISSUE BIOREACTOR - In one embodiment, a system includes a bioreactor coupled to a substrate. The bioreactor includes: a plurality of walls defining a reservoir; a plurality of fluidic channels in at least some of the walls; a fluidic inlet in fluidic communication with the reservoir via, the fluidic channels; a fluidic outlet in fluidic communication with the reservoir via the fluidic channels; and one or more sensors coupled to the reservoir, each sensor being configured to detect one or more of: environmental conditions in the reservoir; and physiological conditions of one or more cells optionally present in the reservoir. In another embodiment, a method includes delivering media to a reservoir of a bioreactor; delivering a, plurality of cells to the reservoir, controlling a reservoir temperature and a reservoir gas composition; using one or more of the sensors to monitor environmental and physiological conditions; and reporting the environmental and/or physiological conditions. | 10-30-2014 |
20140322702 | DETECTION OF WEST NILE VIRUS NUCLEIC ACIDS IN THE VIRAL 3' NON-CODING REGION - Methods for detecting | 10-30-2014 |
20140322703 | Measuring Range Extension of Chromatographic Rapid Tests - A method for the quantitative determination of an analyte in a sample is provided comprising: (a) providing an analyte-specific substance which is able to undergo a reaction which generates a detectable signal when it is contacted with an analyte; (b) providing at least two calibration graphs which have been generated by reacting in each case the same analyte-specific substance with different amounts of in each case the same test analyte for in each case a predetermined reaction time; (c) contacting the analyte-specific substance with a sample which contains the analyte to be detected; (d) measuring the signal at a first predetermined reaction time for which a first calibration graph according to (b) is provided; (c) checking whether the signal measured according to (d) enables a quantitative determination of the analyte with a desired accuracy; (f) (i) quantitatively determining the analyte on the basis of the signal measured according to (d) if the desired accuracy is reached, or (ii) measuring the signal at a second predetermined reaction time for which a second calibration graph according to (b) is provided; (g) checking whether the signal measured according to (f(ii)) enables a quantitative determination of the analyte with a desired accuracy; and (h) (i) quantitatively determining the analyte on the basis of the signal measured according to f(ii) if the desired accuracy is reached, or (ii) continuing the determination at at least one further predetermined reaction time (corresponding to (f)(ii), (g), (h)(i)). | 10-30-2014 |
20140322704 | Hepatitis Virus Culture Systems Using Stem Cell-Derived Human Hepatocyte-Like Cells and Their Methods of Use - The invention relates to the discovery that DHH derived from stem cells are permissive for infection by hepatitis viruses (HV), such as hepatitis A virus (HAV), hepatitis B virus (HBV), hepatitis C virus (HCV), hepatitis D virus (HDV) and hepatitis E virus (HEV). Included in the invention are HV-permissive DHHs and methods of making an HCV-permissive DHH derived from a stem cell. Also included is an HV culture system comprising at least one HV-permissive DHH. The HV-permissive DHH and HV culture system are useful for conducting HV life cycle analyses, diagnosing a subject as being infected with HV, genotyping and characterizing the HV of a subject infected with HV, detecting drug resistance of HV obtained from a subject infected with HV, screening for and identify modulators of HV infection, and monitoring the effect of a treatment of HV in a subject. | 10-30-2014 |
20140329225 | TARGET DETECTION WITH NANOPORE - Provided are methods for detecting a target molecule or particle suspected to be present in a sample, comprising (a) contacting the sample with (i) a fusion molecule comprising a ligand capable of binding to the target molecule or particle and a binding domain, and (ii) a polymer scaffold comprising at least one binding motif to which the binding domain is capable of binding, under conditions that allow the target molecule or particle to bind to the ligand and the binding domain to bind to the binding motif; (b) loading the polymer into a device comprising a pore that separates an interior space of the device into two volumes, and configuring the device to pass the polymer through the pore from one volume to the other volume, wherein the device further comprises a sensor adjacent to the pore configured to identify objects passing through the pore; and (c) determining, with the sensor, whether the fusion molecule or particle bound to the binding motif is bound to the target molecule or particle, thereby detecting the presence of the target molecule or particle in the sample. | 11-06-2014 |
20140329226 | METHOD FOR IDENTIFYING MARKERS - The invention is a method for identifying markers associated with the presence of a predetermined characteristic comprising accumulating spectral data from a sample known to have a predetermined characteristic, identifying a spectral feature which is indicative of the predetermined characteristic, and identifying a marker associated with the spectral feature. | 11-06-2014 |
20140329227 | METHODS OF REDUCING OR ELIMINATING PROTEIN MODIFICATION AND DEGRADATION ARISING FROM EXPOSURE TO UV LIGHT - Methods of inactivation of a virus in a sample comprising a protein component are provided. Also provided are methods of reducing protein degradation or modification in to the presence of a reactive species, such as a reactive species generated as a result of UV exposure, are also provided. In another aspect, a method of reducing oxidation of methionine residues, tryptophan residues or both methionine and tryptophan residues in a protein subjected to UV light is provided. The disclosed methods can be performed at any scale and can be automated as desired. | 11-06-2014 |
20140329228 | FLUORESCENCE IMMUNOASSAY USING POLYPEPTIDE COMPLEX CONTAINING FLUORO-LABELED ANTIBODY VARIABLE REGION - An object of the present invention is to provide an immunoassay that enables the rapid and convenient quantitative measurement with high detection sensitivity of a target substance in a liquid phase without the need of immobilization and washing steps and enables the visualization of an antigen. The present invention achieves the object by performing sequentially the steps of:
| 11-06-2014 |
20140329229 | ABSORBENT DRIED BIOFLUID COLLECTION SUBSTRATES - A new device and methods that allow for improved sequestration and preservation of harvested analytes and bio-molecules from biofluid samples is defined. The new device and methods relate to an improved dried biofluid collection substrate that is absorbent and contains a plurality of affinity ligands located within defined sample collection regions for enhanced analyte collection and storage. The device and methods allow for simple, safe and reliable ambient temperature collection and preservation of molecules captured from biological and environmental fluids in quantities suitable for analysis and diagnostic testing. | 11-06-2014 |
20140329230 | METHODS FOR DETECTING HUMAN PAPILLOMAVIRUS AND PROVIDING PROGNOSIS FOR HEAD AND NECK SQUAMOUS CELL CARCINOMA - Methods and kits for diagnosing and determining prognosis of head and neck squamous cell carcinoma are described. An exemplary method for diagnosing head and neck squamous cell carcinoma (HNSCC) in a subject may comprise assaying a saliva sample from the subject for the presence of total protein, solCD44, and HPV, and using the combination of total protein, HPV, and CD44 levels in a multivariate analysis to determine a combined score, whereby an increase in score above a cut-off point distinguishes subjects with HNSCC, or an elevated risk of future occurrence thereof, from subjects without HNSCC or at low risk of future occurrence thereof. | 11-06-2014 |
20140329231 | LIQUID MEDIUM AND SAMPLE VIAL FOR USE IN A METHOD FOR DETECTING CANCEROUS CELLS IN A CELL SAMPLE - The current invention concerns a method for determining the presence of a (pre)cancerous cell in a liquid cell sample, comprising the steps of: —suspending and preserving a cell sample obtained from a subject in a sample vial comprising a liquid medium, said liquid medium comprises means for labeling cells or epitope(s) on or in said cells; —obtaining data from said labelled liquid cell sample; and —determining the presence of said (pre)cancerous cells based on said obtained data; characterized in that said data comprises morphological data and biomarker data. In a further aspect, the invention relates to a liquid medium for fixing, preserving and labeling cells in a cell sample and a sample vial specifically designed to be used in conjunction with the current method. | 11-06-2014 |
20140329232 | METHOD FOR THE DETECTION OF NUCLEIC ACID SYNTHESIS AND/OR AMPLIFICATION - The present invention relates to a method for the detection of nucleic acid synthesis and/or amplification, characterised in that the method includes adding at least one colorimetric metal indicator and at least one bland magnesium chelator to a reaction mixture for nucleic acid amplification. The present invention further relates to a kit for carrying out such a method and to the use thereof in the health, food and agricultural or veterinary fields. | 11-06-2014 |
20140335504 | HUMAN ADAPTATION OF H5 INFLUENZA - The present invention provides, among other things, technologies and methodologies for detection, treatment, and/or prevention of influenza transmission and/or infection. The present invention also provides technologies for monitoring influenza HA variants with particular degrees of susceptibility to mutation for human adaptation. | 11-13-2014 |
20140335505 | SYSTEMS AND METHODS FOR COLLECTING AND TRANSMITTING ASSAY RESULTS - Systems and methods are provided for collecting, preparing, and/or analyzing a biological sample. A sample collection site may be utilized with one or more sample processing device. The sample processing device may be configured to accept a sample from a subject. The sample processing device may perform one or more sample preparation step and/or chemical reaction involving the sample. Data related to the sample may be sent from the device to a laboratory. The laboratory may be a certified laboratory that may generate a report that is transmitted to a health care professional. The health care professional may rely on the report for diagnosing, treating, and/or preventing a disease in the subject. | 11-13-2014 |
20140335506 | MODULAR POINT-OF-CARE DEVICES, SYSTEMS, AND USES THEREOF - The present invention provides devices and systems for use at the point of care. The methods devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device are modular to allow for flexibility and robustness of use with the disclosed methods for a variety of medical applications. | 11-13-2014 |
20140335507 | ASSAYS FOR INFLUENZA VIRUS HEMAGGLUTININS - The invention quantifies HA using a process which comprises two key steps: HA is captured using a lectin, such as snowdrop lectin; and captured HA is quantified using an immunoassay. This assay can conveniently be performed in an ELISA format. The process is specific, is robust in the face of contaminants, and is more rapid and more sensitive than the standard SRID assay. | 11-13-2014 |
20140335508 | METHOD FOR DETECTING AN INFECTION BY THE HEPATITIS C VIRUS - The invention relates to a method of in-vitro detection of an infection with a hepatitis C virus (HCV) in a biological sample, comprising the simultaneous detection of the HCV capsid protein, and of an antibody directed against said capsid protein, said method using, for capturing the anti-capsid antibodies, a peptide comprising an antigenic fragment derived from the truncated HCV capsid. The invention also relates to the peptide for capturing the anti-capsid antibodies and the kits comprising it. | 11-13-2014 |
20140342349 | USE OF AT LEAST ONE BIOMARKER FOR THE IN VITRO PROGNOSIS OR DIAGNOSIS OF LYMPHOPROLIFERATIVE EPISODES ASSOCIATED WITH THE EPSTEIN-BARR VIRUS (EBV) - A protein complex isolated from its natural medium and including the ZEBRA protein of sequence SEQ ID NO: 1, the isolated protein complex having the following properties:
| 11-20-2014 |
20140342350 | MIXING OF FLUIDS IN FLUIDIC SYSTEMS - Fluidic devices and methods associated with mixing of fluids in fluidic devices are provided. In some embodiments, a method may involve the mixing of two or more fluids in a channel segment of a fluidic device. The fluids may be in the form of, for example, at least first, second and third fluid plugs, composed of first, second, and third fluids, respectively. The second fluid may be immiscible with the first and third fluids. In certain embodiments, the fluid plugs may be flowed in series in the channel segment, e.g., in linear order, causing the first and third fluids to mix without the use of active components such as mixers. The mixing of fluids in a channel segment as described herein may allow for improved performance and simplification in the design and operations of fluidic devices that rely on mixing of fluids. | 11-20-2014 |
20140342351 | COMPOSITIONS AND REACTION MIXTURES FOR THE DETECTION OF NUCLEIC ACIDS FROM MULTIPLE TYPES OF HUMAN PAPILLOMAVIRUS - Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed. | 11-20-2014 |
20140349275 | METHODS AND COMPOSITIONS FOR DETECTING BK VIRUS - The invention provides methods and compositions for rapid, sensitive, and highly specific nucleic acid-based (e.g., DNA based) detection of a BK virus in a sample. In general, the methods involve detecting a target nucleic acid having a target sequence of a conserved region of BK viral genomes. The invention also features compositions, including primers, probes, and kits, for use in the methods of the invention. | 11-27-2014 |
20140349276 | SIGNAL PROPAGATION BIOMOLECULES, DEVICES AND METHODS - This disclosure describes a structured polynucleotide, devices that include the structured polynucleotide, and methods involving the structured polynucleotide and/or devices. Generally, the structured polynucleotide includes five domains. A first domain acts as a toehold for an input DNA logic gate to initiate binding to an SCS biomolecule. A second domain acts as a substrate recognition sequence for an upstream DNA logic gate. A third domain acts as a toehold for a output DNA logic gate to initiate binding of the SCS biomolecule to the gate. A fourth domain acts as an effector sequence to alter the state of the output logic gate. A fifth domain acts as a cage sequence to lock the effector sequence in an inactive state until an input gate binds to the structured polynucleotide. | 11-27-2014 |
20140349277 | ANTI-HUMAN NOROVIRUS GII ANTIBODY - Provided is an anti-human-norovirus GII antibody which responds to substantially all genotypes of the human noroviruses belonging to GII and which can comprehensively detect such human noroviruses GII. | 11-27-2014 |
20140349278 | NANOHOLE SENSOR CHIP WITH REFERENCE SECTIONS - A device and method for detecting and assessing the quantity of a biological, biochemical, or chemical analyte in a test sample using a simple light source and the naked eye are disclosed. In one embodiment, the device comprises a nanohole sensor chip with two sections, the first of which is a test section, upon which capture agents for a particular analyte are immobilized, and the second of which is a reference section, upon which capture agents conjugated with known quantities of the analyte are immobilized. In another embodiment of the invention, a nanohole sensor chip with a test section and a plurality of reference sections is disclosed. The sensor utilizes light intensity changes exhibited by Fano resonances in the nanoholes for detection of analytes, and allows comparison between the light intensity changes between the reference sections and the test sections for assessing the quantity of the analyte in the sample. | 11-27-2014 |
20140349279 | 3D MICROFLUIDIC SYSTEM HAVING NESTED AREAS AND A BUILT-IN RESERVOIR, METHOD FOR THE PREPARING SAME, AND USES THEREOF - The present invention relates to a three dimensional (or 3D) microfluidic system comprising a plurality of layers stacked upon each other, characterised in that at least one of said layers consists of a 1 | 11-27-2014 |
20140349280 | METHOD FOR DETECTING AND DIRECTLY IDENTIFYING A MICROORGANISM IN A BIOLOGICAL SAMPLE BY AN OPTICAL ROUTE - The present invention relates to the field of analysis, for example biological analysis. Specifically, the invention relates to a method for detecting at least one microorganism present in a sample, the method essentially including the following steps: a) in a first container, bringing the sample into contact with at least one culture medium, b) placing the first container in suitable conditions to permit growth of the microorganism or microorganisms, c) bringing some or all of the mixture being made of the sample and the culture medium into contact with a reaction mixture and a substrate for capturing the microorganism(s) in the first container or in a second container, the reaction mixture having a device for detecting the microorganism(s); d) detecting, within the first or second container, the presence of the microorganism or microorganisms detected by the detecting device and fixed on the capture substrate. | 11-27-2014 |
20140356856 | Method for Manufacturing and Using a Test Paper and Chemical Composition Thereof - A method for manufacturing a test paper is disclosed in the present invention, and at least comprises the following steps. First, a chemical precursor comprising at least a reducing agent is coated onto a substrate. The substrate is then dipped into a metal salt solution comprising a plurality of metal ions for a predetermined time to reduce the metal ions to form metal particles on the substrate. Finally, the substrate is taken out and dried to complete a manufacture of at least a test paper. In the meantime a method for using the test paper, and a chemical composition used in the abovementioned for manufacturing the test paper are also disclosed in the present invention. | 12-04-2014 |
20140356857 | INTERPARTICLE SPACING MATERIAL INCLUDING NUCLEIC ACID STRUCTURES AND USE THEREOF - Provided is an interparticle spacing material comprising a nucleic acid structure which comprises at least one nucleic acid lattice comprising a double helix domain; and at least one metal particle which is in contact with a plane of the nucleic acid lattice, in a direction extending obliquely or perpendicularly away from the plane; wherein the double helix domain comprises a hybridization area in which a single strand is hybridized with another single strand. | 12-04-2014 |
20140356858 | Biological Tag Tracking of Agricultural Products, Foods, and Other Items - The disclosure relates to compositions and methods for applying a biological tag to a product and detection of the biological tag on the product by another. The compositions and methods are useful for tracing the provenance of a product, such as a food product or an agricultural product. | 12-04-2014 |
20140356859 | DETECTION OF NUCLEIC ACIDS USING UNMODIFIED GOLD NANOPARTICLES - A gold nanoparticle-based colorimetric assay kit for nucleic acids from viral, bacterial and other microorganisms that detects unamplified or amplified polynucleotides in clinical specimens using unmodified AuNPs and oligotargeter polynucleotides that bind to a pathogen's nucleic acids. A method for detecting a pathogen comprising contacting a sample suspected of containing microbes with a polynucleotide that binds to pathogen nucleic acid and with gold nanoparticles, detecting the aggregation of nanoparticles, and detecting pathogen polynucleotides in the sample when the nanoparticles aggregate (solution color becomes blue) in comparison with a control or a negative sample not containing the virus when nanoparticles do not aggregate (solution color remains red). | 12-04-2014 |
20140356860 | METHOD FOR ISOLATING NUCLEIC ACIDS FROM A VETERINARY WHOLE BLOOD SAMPLE - The present invention provides a method for isolating nucleic acids from a veterinary whole blood sample, said method comprising at least the following steps a) preparing a binding mixture comprising—the lysed sample—at least one chaotropic agent—at least one alcohol—at least one polyoxyethylene fatty alcohol ether; b) passing the binding mixture through a column comprising a nucleic acid binding solid phase thereby binding the nucleic acids to the nucleic acid binding solid phase; c) optionally washing the nucleic acids while being bound to the solid phase; d) optionally eluting the nucleic acids from the solid phase. It was found that the addition of the specific non-ionic detergent overcomes the problems of the prior art methods, wherein the column clogs what prevents the efficient nucleic acid isolation from this difficult sample. When the specific non-ionic detergent is included into the binding mixture, no clogging occurs thereby allowing the efficient isolation of nucleic acids from veterinary whole blood samples. | 12-04-2014 |
20140356861 | METHOD FOR CHECKING THE SELF-HEALING PROCESS CARRIED OUT BY THE IMMUNE SYSTEM OF A TEST SUBJECT INFECTED WITH HUMAN PAPILLOMA VIRUS - A method and device for checking the self-healing process carried out by the immune system of a test subject infected with human papillomavirus (HPV) using one or more cell-containing samples taken from the test subject, to prevent the formation of an HPV-induced carcinoma. The method involves: (1) determining whether HPV with at least one HPV-specific stimulus molecule, stimulating the test subject's immune system, is contained in at least one of the samples; (2) determining whether innate HPV antibodies with the stimulus molecule are in the sample or at least one of the other samples; (3) analyzing results from (1) and (2) to determine whether the self-healing process has started based on the presence of the stimulus molecule and/or the antibodies. | 12-04-2014 |
20140356862 | SYSTEM FOR ON-LINE MONITORING AND CONTROLLING OF CHEMICAL REACTIONS IN REACTORS - The application describes an MRD-based reactor. The reactor is characterized by a continuous wall portion, and is in connection with a MRD, adapted for performing localized NMR spectroscopy of the medium inside the reactor. MRD-based reactors, in which the MRD is at least partially inside the reactor or reaction media, and those in which the MRD accommodates the reactor, are also introduced. Lastly, the invention teaches an in situ method for controlling and analyzing of a reaction. The method makes use of an MRD-based reactor; and comprises applying a magnetic field within the reactor, especially for performing a plurality of localized spectroscopic measurements and either real time or offline analyzing and/or controlling of reactions in the flowing media. | 12-04-2014 |
20140356863 | METHODS FOR DETERMINING THE SUSCEPTIBILITY OF A VIRUS TO AN ATTACHMENT INHIBITOR - The present invention provides novel compositions and methods that can be used for determining the susceptibility of virus to an attachment inhibitor. The present invention also provides methods for predicting whether an infected individual will respond to treatment with an attachment inhibitor, thereby allowing an effective treatment regimen to be designed without subjecting the patient to unnecessary side effects. | 12-04-2014 |
20140356864 | ANALYSIS OF COLORIMETRIC OR FLUOROMETRIC TEST ASSAYS - The present invention provides for the analysis of colorimetric or fluorometric assays by way of capturing an image of the assay on the camera ( | 12-04-2014 |
20140363807 | Genetic Marker for Detection of Human Papillomavirus - The invention provides compositions and methods for the differential detection of high risk forms of HPV from a urine sample provided by a patient. Specifically, the invention provides primers and probes that specifically recognize and bind sequences within the E1 gene of HPV. Detection of high risk forms of HPV identifies individuals at risk of developing or in the early stages of cervical carcinoma. | 12-11-2014 |
20140363808 | APTAMERS SCREENING METHOD BASED ON GRAPHENE WITHOUT TARGET IMMOBILIZATION AND THE APTAMERS OBTAINED FROM THE METHOD - Provided is aptamers screening method based on graphene without target immobilization and the aptamers obtained from the method, and more particularly, a new GO-SELEX method without target immobilization in which a single-stranded nucleic acid pool may react with a non-bound target material or a counter-target material, after which a single-stranded nucleic acid which has not been bound to the target or counter-target may be separated by using the graphene. Also, the specific aptamer obtained through the above-described method may be used for diagnosing target related diseases. | 12-11-2014 |
20140363809 | COMPOSITIONS AND METHODS FOR DETECTING CERTAIN FLAVIVIRUSES INCLUDING MEMBERS OF THE JAPANESE ENCEPHALITIS VIRUS SEROGROUP - The present invention provides rapid and accurate methods, primers, probes and kits for identifying the presence of a certain flaviviruses in a sample. Flaviviruses that can be detected include members of the Japanese encephalitis virus serogroup, Dengue virus, St. Louis encephalitis virus, Montana myotis leukoencephalitis virus, Modoc virus, and Yellow Fever virus. The primers and probes of the invention can hybridize to regions in the 3′ untranslated region of the viral genomes to be detected. | 12-11-2014 |
20140363810 | METHOD OF MEASURING HUMAN CYP3A INDUCIBILITY - A method for measuring human CYP3A inducibility upon administration of a test drug, characterized in that a non-human animal to which a test drug is administered or a population of human cells cultured in a medium containing a test drug is infected with viruses (A) and (B); virus (A) being an adenovirus which is used as a vector and engineered by incorporating thereto a detectable reporter gene and at least 3 human PXR binding regions falling within an untranslated region of a human CYP3A gene, and virus (B) being an adenovirus which is used as a vector and engineered by incorporating thereto a human PXR cDNA; and subsequently expression level of the reporter gene is determined in the non-human animal or the cultured human cells. | 12-11-2014 |
20140370494 | EXOGENOUS INTERNAL POSITIVE CONTROL - The present invention provides an internal positive control for contaminating viruses. The invention provides the use of a second virus as an exogenous internal positive control in methods for verifying the reliability of an assay to detect a first virus, in methods of ensuring the absence of the first virus in a biological sample or pharmaceutical sample and in methods of manufacturing a vaccine free from a first virus. | 12-18-2014 |
20140370495 | RECOMBINANT MYCOBACTERIOPHAGES FOR DELIVERY OF NUCLEIC ACIDS OF INTEREST INTO MYCOBACTERIA - Methods are provided for detecting mycobacteria in a sample, including clinical samples. Methods are also provided for determining susceptibility of mycobacterial strains to known or potential antibiotic agents, as are kits therefor. Recombinant mycobacteriophages are also provided comprising heterologous nucleic acids of interest. | 12-18-2014 |
20140370496 | OLIGONUCLEOTIDE FOR HIV DETECTION, HIV DETECTION KIT, AND HIV DETECTION METHOD - The present invention provides an oligonucleotide for HIV detection including a base sequence which is at least 80% identical to a base sequence composed of 10 or more continuous bases in a base sequence represented by SEQ ID NO. 1 or 6, and an HIV detection kit and an HIV detection method that uses the oligonucleotide for HIV detection. | 12-18-2014 |
20140370497 | METAPNEUMOVIRUS STRAINS AND THEIR USE IN VACCINE FORMULATIONS AND AS VECTORS FOR EXPRESSION OF ANTIGENIC SEQUENCES - Provided is an isolated mammalian negative strand RNA virus, metapneumovirus (MPV), within the sub-family Pneumoviridae, of the family Paramyxoviridae. Also provided are isolated mammalian negative strand RNA viruses identifiable as phylogenetically corresponding or relating to the genus Metapneumovirus and components thereof. In particular, provided is a mammalian MPV, subgroups and variants thereof. Also provided are genomic nucleotide sequences of different isolates of mammalian MPV, in particular, human MPV. Disclosed is the use of the sequence information of different isolates of mammalian MPV for diagnostic and therapeutic methods. Provided are nucleotide sequences encoding the genome of an MPV or a portion thereof, including both mammalian and avian MPV. Further described are chimeric or recombinant viruses encoded by the nucleotide sequences and chimeric and recombinant mammalian MPV that comprise one or more non-native or heterologous sequences. Also provided are vaccine formulations comprising mammalian or avian MPV, including recombinant and chimeric forms thereof. The vaccine preparations encompass multivalent vaccines, including bivalent and trivalent vaccine preparations. | 12-18-2014 |
20140377740 | SOLUBLE AND MEMBRANE-ANCHORED FORMS OF LASSA VIRUS SUBUNIT PROTEINS - Soluble and membrane-anchored forms of Lassa virus (LASV) glycoprotein 1 (GP1), glycoprotein 2 (GP2), the glycoprotein precursor (GPC), the nucleocapsid protein (NP), and the nucleic acids encoding these proteins are disclosed, as well as diagnostic and preventative methods using these compositions. Also disclosed are methods including preparation of vaccines, factors (e.g. small molecules) that inhibit LASV infectivity, and diagnostic and therapeutic antibodies including neutralizing antibodies for the prevention and treatment of infection by LASV and other arenaviruses. | 12-25-2014 |
20140377741 | USE OF FOCUSED LIGHT SCATTERING TECHNIQUES IN BIOLOGICAL APPLICATIONS - Methods for using focused light scattering techniques for the optical sensing of biological particles suspended in a liquid medium are disclosed. The optical sensing enables one to characterize particles size and/or distribution in a given sample. This, in turn, allows one to identify the biological particles, determine their relative particle density, detect particle shedding, and identify particle aggregation. The methods are also useful in screening and optimizing drug candidates, evaluating the efficacy and dosage levels of such drugs, and in personalized medicine applications. | 12-25-2014 |
20150010900 | MULTIPLE- ANALYTE ASSAY DEVICE AND SYSTEM - Provided herein are systems and methods for performing complex chemical, physical and biological assays. In particular, provided herein are systems and methods for performing microfluidic assays. | 01-08-2015 |
20150010901 | METHOD FOR DETECTING POLYOMAVIRUS REACTIVATION - The invention provides methods of detection and monitoring of polyomavirus reactivation and active polyomavirus infections using a biological fluid sample. Also provided are methods of risk assessment and risk monitoring of developing a polyomavirus-associated disease. | 01-08-2015 |
20150010902 | Apparatus and Method for Monitoring Airborne Microorganisms in the Atmosphere - Disclosed is an apparatus for monitoring airborne microorganisms composed of: a chassis, which has a fan for flowing the air therein from the outside at a portion thereof and of which inside is spatially segmented by partition plates for performing a plurality of steps; a perforated plate, which is disposed at a portion of the chassis and has a plurality of nozzles for focusing split air flows passing through a plurality of spaces in a given direction; a capturing plate, which has a plurality of trapping surfaces at the positions opposite to the plurality of nozzles of the perforated plate; a capturing plate control part, which moves the capturing plate relative to the perforated plate; and an optical detection part for fluorescence generated from the microorganisms on the trapping surface of the capturing plate. The apparatus for monitoring airborne microorganism is characterized in that air containing microorganisms flows into some of the plurality of spaces segmented in the chassis, each of the plurality of trapping surfaces no the capturing plate has a pillar, and the capturing plate control part controls the position of the capturing plate; thereby, the air flows hit sequentially against the plurality of trapping surfaces of the capturing plate through the plurality of nozzles of the perforated plate from the plurality of spaces segmented in the chassis and the optical detection part detects sequentially fluorescence from the trapping surfaces of the capturing plate to detect the microorganisms for monitoring the presence or absence thereof. | 01-08-2015 |
20150010903 | Real Time Diagnostic Assays Using an Evanescence Biosensor - The present invention relates to a method for the detection of a substance in an aqueous, physiological or chemical liquid using the evanescence field method, and to a diagnostic device for carrying out said method. | 01-08-2015 |
20150017629 | DEVICE AND METHOD FOR BACTERIAL CULTURE AND ASSAY - The present invention provides a simple culture device that is designed for manufacture and use in areas of limited resources. The device is useful for cell culture in such environments with limited resources because cells grow in paper just as they do in a culture dish. Also provided is a binding assay that employs an activatable dormant bacteriophage carrying a reporter gene to qualitatively or quantitatively detect the presence of a substance of interest in a sample. | 01-15-2015 |
20150017630 | SIGNATURES AND DETERMINANTS FOR DIAGNOSING INFECTIONS AND METHODS OF USE THEREOF - Antibiotics (Abx) are the world's most misused drugs. Antibiotics misuse occurs when the drug is administered in case of a non-bacterial infection (such as a viral infection) for which it is ineffective. Overall, it is estimated that 40-70% of the worldwide Abx courses are mis-prescribed. The financial and health consequences of Abx over-prescription include the direct cost of the drugs, as well as the indirect costs of their side effects, which are estimated at >$15 billion annually. Furthermore, over-prescription directly causes the emergence of Abx-resistant strains of bacteria, which are recognized as one of the major threats to public health today. This generates an immediate need for reliable diagnostics to assist physicians in correct Abx prescription, especially at the point-of-care (POC) where most Abx are prescribed. Accordingly, some aspects of the present invention provide methods using biomarkers for rapidly detecting the source of infection and administrating the appropriate treatment. | 01-15-2015 |
20150017631 | REAL-TIME PCR DETECTION OF SEASONAL INFLUENZA H1, H3 and B SUBTYPES - The present invention relates to assays, diagnostic kits and methods for the simultaneous real-time PCR detection of influenza viruses selected from influenza A, in particular subtypes H1 and/or H3, and/or influenza B. | 01-15-2015 |
20150024376 | TRAP AND FLOW SYSTEM AND PROCESS FOR CAPTURE OF TARGET ANALYTES - A magnetizable trap and flow system and process are detailed that uniformly disperse paramagnetic or superparamagnetic analyte capture beads within a scaffold of magnetizable beads or other magnetizable materials in a capture zone that provides selective capture of target analytes. A magnet placed or energized in proximity to the trap may magnetize the magnetizable scaffold and secure the paramagnetic or superparamagnetic analyte capture beads in their uniformly dispersed state within the magnetizable scaffold to provide selective capture of target analytes. | 01-22-2015 |
20150024377 | APPARATUS COMPRISING MAGNETICALLY ACTUATED VALVES AND USES THEREOF - The present invention, in part, relates to an apparatus having a single-use, normally-closed fluidic valve that is initially maintained in the closed position by a valve element bonded to an adhesive coating. The valve is opened using a magnetic force. The valve element includes a magnetic material or metal. In some examples, the valve is opened by bringing a magnet in proximity to the valve element to provide a magnetic force that delaminates the valve element from the adhesive coating. In particular, the apparatus can be useful for on-chip amplification and/or detection of various targets, including biological targets and any amplifiable targets. Such apparatuses and methods are useful for in-field or real-time detection of targets, especially in limited resource settings. | 01-22-2015 |
20150024378 | Analysis of DNA-Containing Samples and Resolution of Mixed Contributor DNA Samples - Methods for analyzing DNA-containing samples are provided. The methods can comprise isolating a single genomic equivalent of DNA from the DNA-containing sample to provide a single isolated DNA molecule. The single isolated DNA molecule can be subjected to amplification conditions in the presence of one or more sets of unique molecularly tagged primers to provide one or more amplicons. Any spurious allelic sequences generated during the amplification process are tagged with an identical molecular tag. The methods can also include a step of determining the sequence of the one or more amplicons, in which the majority sequence for each code is selected as the sequence of the single original encapsulated target. The DNA-containing sample can be a forensic sample (e.g., mixed contributor sample), a fetal genetic screening sample, or a biological cell. | 01-22-2015 |
20150024379 | FIBER SAMPLER FOR RECOVERY OF BIOAEROSOLS AND PARTICLES - An aerosol collection system and method. The system includes a bio-aerosol delivery device configured to supply bioparticles in a gas stream, a moisture exchange device including a partition member coupled to the gas stream and configured to humidify or dehumidify the bioparticles in the gas stream, and an aerosol collection medium downstream from the moisture exchange device and configured to collect the bioparticles. The method includes delivering bioparticles in a gas stream, humidifying or dehumidifying the bioparticles in the gas stream by transport of water across a partition member and into a vapor phase of the gas stream, and collecting the bioparticles by a collection medium. | 01-22-2015 |
20150024380 | INTERFERING PEPTIDES AND METHOD FOR DETECTING MICROORGANISMS - The invention relates to novel interfering peptides having peptide sequence S with between 7 and 12 amino acids, originating from the peptide sequence of an antigenic protein of a micro-organism M, the sequence S being aligned with a peptide sequence S′ with between 7 and 12 amino acids originating from the peptide sequence of a target protein of a micro-organism M′ that is different from the micro-organism M, provided that: sequences S and S′ have at least 50% identity over their length of 7 to 12 amino acids and at least 4 identical or analogous contiguous amino acids; and their length is identical or they have 1 or 2 different amino acids distributed at one and/or the other end of the sequences. The invention also relates to a method for the in vitro immunoassay-based detection of the presence of a micro-organism M′ or M in a biological sample. | 01-22-2015 |
20150024381 | DETECTING AND QUANTIFYING CRYPTIC HIV REPLICATION - The present invention relates to a novel method for detecting efficient cryptic HIV replication in a patient who receives a suppressive antiviral therapy followed by administration of the HIV integrase inhibitor in an effective amount for intensifying the suppressive antiviral therapy, and has undetectable plasma viremia prior to the administration of the HIV integrase inhibitor. The method comprises making a pre-intensification measurement and one or more post-intensification measurements of the concentration of an episomal artifact in samples from the patient, and computing a pre-intensification HIV infection success ratio (R). A pre-intensification HIV infection success ratio (R) sufficiently close to 1 indicates that the patient has the efficient cryptic HIV replication. The method may further comprise quantifying the efficient cryptic HIV replication. | 01-22-2015 |
20150024382 | Assay Methods for MDV-1 - A method for the quantification of a vaccine strain and/or a virulent strain of Marek's Disease Virus Serotype-1 (MDV-1) in a sample from a bird, comprising the steps of: (i) providing a biological sample from the bird and optionally isolating nucleic acid from the biological sample; (ii) subjecting the biological sample of (i) to real-time quantitative PCR (qPCR) comprising: (a) amplification of a region of the pp38 gene within the nucleic acid sample of (i), said region containing a consistent single nucleotide polymorphism (SNP) difference between vaccine and virulent strains of MDV-1; and (b) contacting the amplified nucleic acid of (a) with a detectable nucleic acid probe specific for the SNP of the vaccine strain of MDV-1 and/or a detectable nucleic acid probe specific for the SNP of the virulent strain of MDV-1; and (iii) Measuring changes in the detectable signal produced by the probe of (ii). Methods are also provided for the absolute quantification of vaccine and virulent viruses. | 01-22-2015 |
20150024383 | Assay Methods for MDV-1 - A method for the quantification of a vaccine strain and/or a virulent strain of Marek's Disease Virus Serotype-1 (MDV-1) in a sample from a bird, comprising the steps of: (i) providing a biological sample from the bird and optionally isolating nucleic acid from the biological sample; (ii) subjecting the biological sample of (i) to real-time quantitative PCR (qPCR) comprising: (a) amplification of a region of the pp38 gene within the nucleic acid sample of (i), said region containing a consistent single nucleotide polymorphism (SNP) difference between vaccine and virulent strains of MDV-1, and (b) contacting the amplified nucleic acid of (a) with a detectable nucleic acid probe specific for the SNP of the vaccine strain of MDV-1 and/or a detectable nucleic acid probe specific for the SNP of the virulent strain of MDV-1; and (iii) Measuring changes in the detectable signal produced by the probe of (ii). Methods are also provided for the absolute quantification is of vaccine and virulent viruses. | 01-22-2015 |
20150031014 | DETECTING ANALYTES WITH A PH METER - Provided herein are sensors, kits that include such sensors, and methods for making and using such sensors. The sensors permit detection of a broad array of target molecules, such as nucleic acids (e.g., DNA and RNA), proteins, toxins, pathogens, cells, and metals, and can be used in combination with pH meters and pH paper. Thus, this disclosure provides a new methodology that allows pH meters and pH paper to be used for the detection of analytes other than pH. | 01-29-2015 |
20150031015 | IDENTIFICATION OF POLIOVIRUS STRAINS - The present invention relates to methods for identifying and/or distinguishing polioviral strains, in particular polioviral strains used in vaccine production. The methods are based on selective hybridisation with oligonucleotides, i.e. primers and/or probes, that allow to distinguish between closely related but different polioviral strains on the basis of nucleotidepolymorphisms existing between those polioviral strains. Preferably, the methods employ amplification or amplification-ligation assays for detecting the selective hybridisation. The invention further relates to oligonucleotides for use in the methods of the invention and kits comprising such oligonucleotides and optionally enzymes and buffers for carrying out the methods of the invention. | 01-29-2015 |
20150031016 | Mixed Cell Diagnostic Systems For Detection Of Respiratory, Herpes and Enteric Viruses - The present invention generally relates to the field of diagnostic microbiology, and, more particularly, to compositions and methods for detecting and differentiating one or more viruses or other intracellular parasites present in a specimen. The present invention also provides compositions and methods to evaluate the susceptibility of organisms to antimicrobial agents. | 01-29-2015 |
20150031017 | METHODS FOR DETECTING HUMAN PAPILLOMAVIRUS-ASSOCATED CANCERS - The present invention provides probes and methods of use thereof in the diagnosis and/or prognosis of certain types of cancers, particularly human papillomavirus (HPV)-associated cancers. The probes are designed for hybridization with genomic material in a manner indicative of one or more aberrations in the genetic material present in the test sample. The identified aberrations are biomarkers of HPV-associated cancer. The methods of the invention comprise contacting a sample to one or more probes, allowing any genetic material in the sample to hybridize to the genomic regions provided in the probes, analyzing the hybridizations, and analyzing the hybridizations to identify detected aberrations as biomarkers indicative of HPV-associated cancer progression. | 01-29-2015 |
20150031018 | Methods For Diagnosing Feline Coronavirus Infections - Provided is a method for determining whether a feline is infected with pathogenic Feline Infectious Peritonitis Virus (FIPV) or Feline Enteric Infection Virus (FECV). The method involves determining the presence or absence of intact or mutated S1/S2 and S2′ cleavage sites in the spike protein of serotype 1 feline coronaviruses (FCoV1). The presence of both intact cleavage sites is indicative of FECV. The presence of a mutation in one or both cleavage sites is indicative of FIPV. The absence of both sites is indicative of an absence of FCoV1 infection. Compositions for use in determining infection and kits are also provided. | 01-29-2015 |
20150037785 | HYBRIDIZATION ASSAY DETECTION PROBES FOR DETECTING HUMAN PAPILLOMA VIRUS IN A SAMPLE - Hybridization assay detection probes targeted to HPV Type 16 nucleic acid sequences which are particularly useful to aid in detecting HPV type 16 are described. The oligonucleotides can aid in detecting HPV Type 16 by acting singly or as part of a detection probe mixture. Compositions, reaction mixtures and methods of use are provided. | 02-05-2015 |
20150037786 | HANDHELD DIAGNOSTIC SYSTEM WITH CHIP-SCALE MICROSCOPE AND AUTOMATED IMAGE CAPTURE MECHANISM - A handheld diagnostic system may include a disposable sample holder for receiving and containing a biological sample and an analysis module having a chip-scale microscope. The sample holder may include a plurality of uniformly spaced tick marks. The analysis module may include a sensor for detecting the tick marks as the sample holder is inserted into the analysis module. The chip-scale microscope may include an image sensor for capturing images of the sample. Each time the sensor detects a tick mark, control circuitry may issue a control signal to the image sensor to capture an image of the biological sample. This type of automated image capture mechanism ensures that images are captured at a uniform spatial distribution even when the sample holder is inserted into the analysis module at variable speed. The analysis module may transmit sample imaging data to a portable electronic device. | 02-05-2015 |
20150044665 | TARGET-SPECIFIC PROBE COMPRISING T7 BACTERIOPHAGE AND DETECTING FOR BIOMARKER USING THE SAME - The present invention relates to a target-specific probe containing T7 bacteriophage with a targeting antibody, and a detection method or a detection kit for a biomarker using the target-specific probe. The biomarker can be detected by using the genetically-modified T7 bacteriophage expressing various heterogeneous proteins and peptides on its surface and antibody-antigen specific reaction which can make the probe targeted to a biomarker or bacteria; and a detectable labeling agent, for example a quantum dot. | 02-12-2015 |
20150044666 | NON-EQUILIBRIUM TWO-SITE ASSAYS FOR LINEAR, ULTRASENSITIVE ANALYTE DETECTION - Methods and kits related to non-equilibrium, ultrasensitive two-site assays for detecting analytes are provided. In one aspect, a two-site assays for detecting analytes under non-equilibrium analyte binding conditions, using low concentrations of reporter specificity molecule (e.g., reporter antibody) and kits for performing the same is provided. In another aspect, methods for selecting antibodies or specificity molecules with low dissociation constants for use as reporter antibodies in non-equilibrium two-site immunoassays, including two-site immuno-PCR assays, and assays performed with those antibodies, are also provided. | 02-12-2015 |
20150044667 | HUMAN PAPILLOMAVIRUS 16 (HPV16) - RELATED EPILEPSY - Human papillomavirus 16 (HPV16) is identified as a cause of epilepsy. This application is directed to HPV16-related epilepsy, methods of detection and prenatal prevention, and immunogenic, therapeutic and prophylactic compositions for same. The methods of predicting or detecting human papillomavirus (HPV16)-associated epilepsy may comprise contacting a biological sample obtained from a human subject, said human subject having experienced one or more seizures, with a diagnostic reagent that can detect HPV16 or an antigen thereof, or a humoral or cell-mediated response to HPV16 or an antigen thereof, in said biological sample, wherein the presence of HPV16 or an antigen thereof or a humoral or cell-mediated response to HPV16 or an antigen thereof, is indicative of HPV16-associated epilepsy. Also provided are parallel methods of diagnosing a structural brain defect in a subject having one or more seizures. | 02-12-2015 |
20150044668 | METHODS FOR DETECTION OF ANTI-CYTOMEGALOVIRUS NEUTRALIZING ANTIBODIES - The present disclosure provides methods useful for determining levels of HCMV infection in host cells and, by extension, determining levels of neutralizing antibodies present in a sample. The present disclosure encompasses the recognition that HCMV viruses that have a fluorescent moiety permit detection of viral infection (e.g., by assessing fluorescence in cells after contacting the host cell with the virus). In some embodiments, levels of HCMV infection are determined by fluorescence detection where the virus has been preincubated with a test sample (e.g., a serum sample) from a subject. In some embodiments, the subject has been administered a candidate HCMV vaccine. | 02-12-2015 |
20150044669 | METHOD FOR THE DETECTION OF BIOLOGICAL MOLECULES USING A TWO PARTICLE COMPLEX - Methods, compositions and kits for detecting analytes of interest in a sample using electrogenerated chemiluminescence are provided. Compositions comprising at least one solid support that entraps or contains an electrogenerated chemiluminescent moiety also provided. | 02-12-2015 |
20150050639 | MOLECULAR METHOD FOR UNIVERSAL DETECTION OF CITRUS VIROIDS - The present invention provides methods for universally detecting citrus viroids in plant material such as germplasm. In particular embodiments, the invention enables the determination of citrus viroid infection and plant resistance. Accordingly, the present method provides methods for improved universal detection of any citrus viroid. | 02-19-2015 |
20150050640 | NOVEL INTERFERON-LAMBDA4 (IFNL4) PROTEIN, RELATED NUCLEIC ACID MOLECULES, AND USES THEREOF - The invention is related to identification of an interferon-analog (IFNL4) protein and genetic association with spontaneous clearance of HCV infection and response to treatment for HCV infection. | 02-19-2015 |
20150056606 | TISSUE TESTER - A tissue tester for providing an indication of the presence of disease including a first paper tissue sheet; and a filter patch treated with a color changing indicator affixed to the first paper tissue sheet. The tester may also consist of a single sheet of filter paper treated with a solution of turmeric and alcohol. The color indicator changes from yellow to brown when disease is indicated in respiratory secretions. | 02-26-2015 |
20150056607 | FLOW CYTOMETER WITH DIGITAL HOLOGRAPHIC MICROSCOPE - The current invention concerns a flow cytometric system and method for observing, analyzing and/or separating objects in a liquid sample, comprising a digital holographic microscope (DHM) and at least one fluidic system, whereby the DHM comprises illumination means, an interferometric system and digital recording means, whereby the fluidic system is capable of guiding said objects through an illumination beam of the illumination means of said DHM, whereby the fluidic system comprises a mechanism for inducing a liquid sample stream through the fluidic system, whereby preferably the fluidic system comprises a stream size controlling device for controlling the transverse dimensions of a liquid sample stream inside said fluidic system, preferably said stream size controlling device is capable of lining up the objects one-by-one or multiple objects at a time in said liquid sample stream. | 02-26-2015 |
20150056608 | Method for Treating Infectious Diseases Using Emissive Energy - The present invention relates to the treatment of infectious diseases, specifically by extracorporeally eradicating the pathogen. This invention comprises methods for the extracorporeal treatment of infectious diseases that will remove infectious pathogens (leukemia cells, bacteria, viruses, or fungi causing a septicemia, metastatic cancer cells, target protein, viruses, parasites, fungi and prions) in humans by targeting such pathogens with a laser or other high-energy source of emissive radiation. More specifically, the method involves removing a bodily fluid from a patient, attaching an antibody to pathogens in the bodily fluid, sensing the antibody-pathogen moiety, using a high-powered, focused laser to destroy the antibody-pathogen moiety, removing the remains of the antibody-pathogen by filtering or other suitable mechanism(s), and returning the bodily fluid to the patient. | 02-26-2015 |
20150056609 | Next Generation Genomic Sequencing Methods - Disclosed is an enhanced method for rapid and cost-effective analysis of sequences of a microorganism by semi-conductor sequencing, preferably ion-torrent sequencing. This method provides for full length analysis and of multiple areas (e.g. genes) of multiple genomes. These methods identify genetic mutations of a particular gene that are responsible for conferring resistance or sensitivity to an antibiotic or other chemical compound. Multiple different species, strains and/or serotypes of a particular organism are rapidly and efficiently screened and mutations identified along with the complete genome of an organism. By selecting primers pairs of similar size and GC content that produce amplicons with sequences spanning the entire genome, a single PCR reaction analyzed by ion torrent methodology can determine the sequence of a complete genome. Methods are useful to sequences the genomes of viral agents, such as influenza virus, and bacterial agents, such as tuberculosis bacteria. | 02-26-2015 |
20150056610 | MONITORING AND INHIBITING HUMAN IMMUNODEFICIENCY VIRUS INFECTION BY MODULATING HMGB1 DEPENDENT TRIGGERING OF HIV-1 REPLICATION AND PERSISTENCE - Compositions and methods for modulating human immunodeficiency virus (HIV) infection involving substances that inhibit the ability of high mobility box 1 (HMGB1) protein to interact with natural killer (NK) cells. Therapeutic compositions comprising antibodies and drugs, such as glycyrrhizin, which bind to HMGB1. Methods of detecting or monitoring HIV infection involving detection or quantitation of HMGB1 or antibodies specific for HMGB1 in a biological sample. | 02-26-2015 |
20150056611 | Method for Manipulating Magnetic Particles in a Liquid Medium - A method of mixing magnetic particles ( | 02-26-2015 |
20150064689 | SYSTEM AND METHOD FOR ANALYZING TRANSMISSIBILITY OF INFLUENZA - The present disclosure provides systems and methods for determining the transmissibility of a pathogen. The system described herein includes a flow device with a plurality of cell culture chambers. Cells, such as primary human tracheal or bronchial epithelial cells, are cultured within the flow device. Cells within a first cell culture chamber are infected with the pathogen. The pathogen's transmissibility is determined by flowing a gas through the flow device such that the gas flows over the infected cells and then over uninfected cells in a second cell culture chamber of the device. By quantifying the amount of virus present in the cells of the second cell culture chamber after a predetermined amount of time, the transmissibility of the pathogen is determined. | 03-05-2015 |
20150064690 | FACILE LABORATORY METHOD FOR LOCALISING BIOMOLECULES TO THE SURFACE OF CELLS AND VIRUSES - A facile method of localising sulfhydryl (—SH) group containing biomolecules, in particular peptides, to the membranes of living cells and enveloped viruses and a kit for use in a biological laboratory in accordance with the method. | 03-05-2015 |
20150064692 | IMMUNOCHROMATOGRAPHIC TEST STRIP AND DETECTION METHOD USING IMMUNOCHROMATOGRAPHY FOR DETECTING TARGET IN RED BLOOD CELL-CONTAINING SAMPLE - A problem to be solved by the present invention is to provide an immunochromatographic test strip and a detection method using immunochromatography avoiding aggregation of colloidal gold conjugates while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a blood-agglutinating agent and the colloidal gold conjugates as a detection reagent. To solve the problem, the present inventers reviewed a past reagent configuration itself from a completely different viewpoint rather than selecting type and amount of polyanions and, as a result of extensive study on each element, the inventers surprisingly found that aggregation of colloidal gold can be suppressed by using a certain buffer solution without using neutralization by polyanions. | 03-05-2015 |
20150064693 | SYSTEMS AND METHODS FOR DETECTION AND QUANTIFICATION OF ANALYTES - Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. In some embodiments, the reader component communicates with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample. | 03-05-2015 |
20150064694 | MULTIDIMENSIONAL HYDRODYNAMIC FOCUSING CHAMBER - Systems, including apparatus and methods, for the microfluidic manipulation, dispensing, and/or sorting of particles, such as cells and/or beads. The systems may include a shaped focusing chamber and/or a branched diverting mechanism. | 03-05-2015 |
20150072338 | SYSTEMS AND METHODS FOR DETECTING INFECTIOUS DISEASES - Systems, methods, and devices for detecting infections in a clinical sample are provided. Small-volume clinical samples obtained at a point-of-service (POS) location and may be tested at the POS location for multiple markers for multiple diseases, including upper and lower respiratory diseases. Samples may be tested for cytokines, or for inflammation indicators. Dilution of samples, or levels of detection, may be determined by the condition or past history of a subject. Test results may be obtained within a short amount of time after sample placement in a testing device, or within a short amount of time after being obtained from the subject. A prescription for treatment of a detected disorder may be provided, and may be filled, at the POS location. A bill may be automatically generated for the testing, or for the prescription, may be automatically sent to an insurance provider, and payment may be automatically obtained. | 03-12-2015 |
20150072339 | METHODS AND KITS FOR QUANTIFYING THE REMOVAL OF MOCK VIRUS PARTICLES FROM A PURIFIED SOLUTION - The present invention relates to a method of quantifying the amount of Mock Virus Particles (MVP) removed from a solution as a result of processing that solution through a purification technique. This method involves the steps of adding MVP to a solution, processing the solution through a purification technique, quantifying the amount of MVP removed from the solution. The present invention also relates to a kit that can be used in conjunction with the method. This kit will comprise at least one stock solution of MVP and at least one quantification solution. | 03-12-2015 |
20150079581 | DETECTION KIT FOR INFLUENZA A VIRUS - The present invention is a test kit for rapidly diagnosing influenza according to the principles of immunochromatography, and the purpose thereof is to provide a test kit for the influenza A virus in which the sensitivity in detecting the influenza A virus is greater than in conventional test kits, and a determination of “positive” is obtained stably and with high precision at an earlier time during the onset of influenza symptoms. The present invention pertains to a kit for detecting influenza A virus, in which an antibody that is in solid phase in the chromatographic medium enters into an antigen-antibody reaction with native nuclear proteins of the influenza A virus, but in Western blots the antibody does not enter into antigen-antibody reactions with full-length nuclear proteins of the influenza A virus that have been separated using SDS-polyacrylamide gel electrophoresis. | 03-19-2015 |
20150079582 | BISMUTH-CONTAINING CONCENTRATION AGENTS FOR MICROORGANISMS - A bismuth-containing concentration agent for microorganisms is provided. Additionally, articles that include the concentration agent and methods of concentrating a microorganism using the concentration agent are provided. | 03-19-2015 |
20150079583 | DEVICE AND METHOD FOR DETECTING A TARGET ANALYTE - One aspect of the present disclosure relates to a calorimeter for detecting the presence of a target analyte in a fluid sample. The calorimeter can include a substrate, a hermetically-sealed, thermally decoupled central reaction zone associated with the substrate, at least one droplet transport region, and detection electronics. The at least one droplet transport region can be associated with the substrate and configured to merge a reagent droplet with a sample droplet including the fluid sample to form a reaction droplet in the central reaction zone. The detection electronics can be in electrical and/or thermal communication with the central reaction zone and associated with the substrate. The calorimeter can be configured to detect a heat of reaction produced by a reaction event between the target analyte and a capture reagent upon formation of the reaction droplet. | 03-19-2015 |
20150086972 | MOLECULAR DETECTION ASSAY - A molecular detection assay including treating a biological sample directly with a bisulphite agent under conditions that allow cell disruption and nucleic acid treatment; removing the bisulphite agent from the treated sample; and detecting a target nucleic acid in the treated sample. | 03-26-2015 |
20150086973 | MONOCLONAL ANTIBODY AGAINST DUCK TEMBUSU VIRUS, HYBRIDOMA CELL LINE AND APPLICATION THEREOF - A monoclonal antibody against the Duck Tembusu virus and a hybridoma cell line secreting the monoclonal antibody, a reagent kit and method for detecting a Duck Tembusu virus antibody, and application of the monoclonal antibody in preparing products for diagnosing the Duck Tembusu virus disease. The monoclonal antibody may bind specifically to E protein of Duck Tembusu virus and has an activity of neutralizing Duck Tembusu virus. | 03-26-2015 |
20150086974 | IMMUNOCHROMATOGRAPHIC TEST STRIP AND DETECTION METHOD USING IMMUNOCHROMATOGRAPHY FOR DETECTING TARGET IN RED BLOOD CELL-CONTAINING SAMPLE - A problem to be solved by the present invention is to provide an immunochromatographic test strip and a detection method using immunochromatography avoiding aggregation of colloidal gold conjugates while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a blood-agglutinating agent and the colloidal gold conjugates as a detection reagent. To solve the problem, the present inventers reviewed a past reagent configuration itself from a completely different viewpoint rather than selecting type and amount of polyanions and, as a result of extensive study on each element, the inventers surprisingly found that aggregation of colloidal gold can be suppressed by using a certain buffer solution without using neutralization by polyanions. | 03-26-2015 |
20150086975 | IMMUNOLOGICAL ANALYSIS METHOD AND REAGENT - Disclosed are an immunoassay method which can measure an antigen with high sensitivity and accuracy; and a reagent therefor. In the immunoassay method, an antigen-antibody reaction and/or a measurement is(are) carried out in the presence of a polycarboxylic acid type surfactant. The immunoassay reagent for use in the method is characterized by comprising the polycarboxylic acid type surfactant. By employing such a simple means that the polycarboxylic acid type surfactant is allowed to be present in the reaction and/or measurement system, non-specific reactions can be suppressed effectively even in a highly sensitive immunoassay, and an antigen can be measured accurately and specificity can be improved in the immunoassay. | 03-26-2015 |
20150086976 | SELF-CATALYTIC REACTION BASED ASSAY - A method for determining the presence or concentration of an analyte in a sample is described, including the steps of associating an initiator with the analyte to form an analyte associated initiator, contacting the analyte associated initiator with a reagent adapted to undergo a self-catalytic reaction which produces a product, and obtaining an observation or measurement corresponding to a change in the amount of the product. Kits and additional methods related to analyte detection are also described. | 03-26-2015 |
20150086977 | GRAPHENE COMPOSITION, METHOD OF FORMING A GRAPHENE COMPOSITION AND SENSOR SYSTEM COMPRISING A GRAPHENE COMPOSITION - A device including a composition formed by oxidation of graphene oxide to form holey graphene oxide having defects therein and reduction of the holey graphene oxide. A composition includes graphene oxide sheets including holes therein formed by oxidation to form a network of interconnected graphene oxide nanoribbons. | 03-26-2015 |
20150086978 | METHODS AND MATERIALS FOR THE DETECTION OF DENGUE VIRUS INFECTION - The present invention provides monoclonal antibodies that are specific for the Dengue non-structural glycoprotein NS1 in monomeric and/or oligomeric (primarily dimeric) form, together with methods, including ELISA and lateral flow assays, that employ the disclosed antibodies for the early detection of Dengue virus infection. Diagnostic kits for the detection of Dengue infection are also provided, such kits including the disclosed monoclonal and/or polyclonal antibodies. | 03-26-2015 |
20150093742 | ANTIBODIES FOR ONCOGENIC STRAINS OF HPV AND METHODS OF THEIR USE - The subject invention provides antibodies, including polyclonal and monoclonal antibodies, that bind to E6 proteins from at least three oncogenic strains of HPV. In general, the antibodies bind to amino acids motifs that are conserved between the E6 proteins of different HPV strains, particularly HPV strains 16 and 18. The subject antibodies may be used to detect HPV E6 protein in a sample, and, accordingly, the antibodies find use in a variety of diagnostic applications, including methods of diagnosing cancer. Kits for performing the subject methods and containing the subject antibodies are also provided. | 04-02-2015 |
20150093743 | ON-DEMAND PARTICLE DISPENSING SYSTEM - Systems, including apparatus and methods, for the microfluidic manipulation, dispensing, and/or sorting of particles, such as cells and/or beads. | 04-02-2015 |
20150093744 | CAPTURE OF HUMAN NOROVIRUS FROM CLINICAL ENVIRONMENTAL AND FOOD SAMPLES AND MEASUREMENT OF INFECTIVITY - The present invention relates to human noroviruses. | 04-02-2015 |
20150093745 | OPTOELECTRONIC DETECTION SYSTEM - The invention relates to optoelectronic systems for detecting one or more target particles. The system includes a reaction chamber, a specimen collector, an optical detector, and a reservoir containing cells, each of the cells having receptors which are present on the surface of each cell and are specific for the target particle to be detected, where binding of the target particle to the receptors directly or indirectly activates a reporter molecule, thereby producing a measurable optical signal. | 04-02-2015 |
20150093746 | Virus Causing Respiratory Tract Illness in Susceptible Mammals - The invention relates to the field of virology. The invention provides an isolated essentially mammalian negative-sense single-stranded RNA virus (MPV) within the subfamily Pneumovirinae of the family Paramyxoviridae and identifiable as phylogenetically corresponding to the genus | 04-02-2015 |
20150099261 | RESPIRATORY INFECTION ASSAY - The present invention provides nucleic acid products and corresponding methods for screening a biological sample for the presence of a respiratory infection-causing microorganism. | 04-09-2015 |
20150099262 | FLOW CYTOMETER BIOSAFETY HOOD AND SYSTEMS INCLUDING THE SAME - Flow cytometer systems are provided that mitigate aerosols generated during operation of a flow cytometer. A flow cytometer system can include various combinations of: a flow cytometer instrument base, a flow cytometer, and a biosafety hood (BSH). In some embodiments, a subject flow cytometer system includes a flow cytometer instrument base, a flow cytometer, and a BSH. In some embodiments, a subject flow cytometer system includes a flow cytometer instrument base and a flow cytometer. In some cases, a BSH includes an aerosol management system, which provides a redundant air filtration system. Also provided are components of a flow cytometer system (e.g., a BSH configured to attach to a flow cytometer instrument base, a flow cytometer instrument base configured to attach to a BSH, etc.). Also provided are methods, including methods of performing a flow cytometric procedure using a flow cytometer system; and methods of decontaminating a flow cytometer system. | 04-09-2015 |
20150099263 | SELECTIVE DETECTION OF HUMAN RHINOVIRUS - A process for detecting human rhinovirus nucleic acid in a biological sample, includes producing an amplification product by amplifying an human bocavirus nucleotide sequence using a forward primer of SEQ ID NO: 1, and a reverse primer of SEQ ID NO: 2, and measuring said amplification product to detect human rhinovirus in said biological sample. Also provided are reagents and methods for detecting and distinguishing human rhinovirus from other viruses. A kit is provided for detecting and quantifying human rhinovirus in a biological sample. | 04-09-2015 |
20150104783 | APPARATUS AND METHOD FOR VIRUS DETECTION - Embodiments of the present invention relate to a method comprising obtaining a radio frequency response of a lab-on-chip based resonator with virus deposited within a recess of the resonator, determining at least one parameter of the radio frequency response and identifying a type of the virus or a group to which the virus belongs based on the at least one parameter. | 04-16-2015 |
20150104784 | MAGNETIC AND/OR ELECTRIC LABEL ASSISTED DETECTION SYSTEM AND METHOD - A detection system ( | 04-16-2015 |
20150111197 | NOVEL ISOLATED AND PURIFIED STRAINS OF CHIKUNGUNYA VIRUS AND POLYNUCLEOTIDES AND POLYPEPTIDES SEQUENCES, DIAGNOSTIC AND IMMUNOGENICAL USES THEREOF - The present invention concerns wild-strains of Chikungunya virus isolated from patients exhibiting severe forms of infection and stemming from a human arbovirosis epidemy. The present invention also concerns polypeptide sequences and fragment thereof derived from their genome, the polynucleotide encoding same and their use as diagnostic products, as vaccine and/or as immunogenic compositions. | 04-23-2015 |
20150111198 | MEDICAL ANALYSIS METHOD - A medical analysis method uses a medical analysis machine provided with a poly-articulated robot ( | 04-23-2015 |
20150111199 | Methods of Using Near Field Optical Forces - Methods of studying, interrogating, analyzing, and detecting particles, substances, and the like with near field light are described. Methods of identifying binding partners, modulators, inhibitors, and the like of particles, substances, and the like with near field light are described. In certain embodiments, the methods comprise immobilizing or trapping the particle, substance, and the like. | 04-23-2015 |
20150111200 | Luminescence Reference Standards - The present teachings provide for systems, and components thereof, for detecting and/or analyzing light. These systems can include, among others, optical reference standards utilizing luminophores, such as nanocrystals, for calibrating, validating, and/or monitoring light-detection systems, before, during, and/or after sample analysis. | 04-23-2015 |
20150111201 | PORTABLE RAPID DIAGNOSTIC TEST READER AND METHODS OF USING THE SAME - A portable rapid diagnostic test reader system includes a mobile phone having a camera and one or more processors contained within the mobile phone and a modular housing configured to mount to the mobile phone. The modular housing including a receptacle configured to receive a sample tray holding a rapid diagnostic test. At least one illumination source is disposed in the modular housing and located on one side of the rapid diagnostic test. An optical demagnifier is disposed in the modular housing interposed between the rapid diagnostic test and the mobile phone camera. | 04-23-2015 |
20150118674 | ANTI-HBC QUANTITATIVE DETECTION METHOD AND USES THEREOF IN MONITORING AND CONTROLLING DISEASE PROGRESSION OF CHRONIC HEPATITIS B PATIENT AND IN PREDICTING THERAPEUTIC EFFECT - The present invention relates to a method for quantitative detection of anti-HBc and its use in monitoring disease progression of chronic hepatitis B patients and predicting therapeutic effects. By quantitative detection of antibodies against hepatitis B core protein (Anti-HBc), it is able to monitor disease progression of chronic hepatitis B patients, effectively predict therapeutic effects in chronic hepatitis B patients who accept a therapy against hepatitis B virus (especially, a therapy based on interferon and a therapy based on nucleoside/nucleotide analogue anti-HBV drug), and thus guide the patients to reasonably choose drugs. | 04-30-2015 |
20150118675 | IMMUNOCHROMATOGRAPHY DETECTION METHOD - The present invention provides an immunochromatography detection method capable of suppressing non-specific reactions. The present invention relates to an immunochromatography detection method including: a step of adding an analyte dilution solution containing an analyte to a chromatography medium; a step of recognizing a detection target by a labeling substance modified with gold nanoparticles, which is dry-retained at a labeling substance retaining part; a step of developing a composite of the labeling substance and the detection target as a mobile phase; and a step of detecting the detection target in the developed mobile phase at a judgment part, wherein the labeling substance is protected with a polyalkylene glycol having one or more mercapto groups and/or a derivative thereof and then dry-retained together with arginine and casein at the labeling substance retaining part. | 04-30-2015 |
20150118676 | ELECTROKINETIC DEVICE FOR CAPTURING ASSAYABLE AGENTS IN A DIELECTRIC FLUID UTILIZING REMOVABLE ELECTRODES - Electrokinetic devices and methods are described with the purpose of collecting assayable agents from a dielectric fluid medium. Electrokinetic flow may be induced by the use of plasma generation at high voltage electrodes and consequent transport of charged particles in an electric voltage gradient. In one embodiment, an ionic propulsion device for providing a sample for a bio-specific assay of aerosol particles comprises a housing receiving a sample of aerosol particles and enclosing a high voltage electrode to generate a plasma of electrically charged particles. A carrier assembly is removably receivable in the housing, the carrier assembly comprising a non-conductive carrier and an electrode removably secured to the carrier. Incident to the carrier assembly being received in the housing, the electrode is subject to a voltage so that flow of charged aerosol particles generates a net air flow through the housing and said charged aerosol particles are deposited on the electrode, and said electrode can be removed from said carrier and placed in an extraction vessel for a bio-specific assay. | 04-30-2015 |
20150125849 | Eukaryotic Expression System And Use Thereof - Provided are a eukaryotic expression system and its applications. The eukaryotic expression system has a recombinant plant cell. The recombinant plant cell includes a first vector and a second vector. The first vector expresses a fusion protein containing an Asia tospoviral common epitope. The fusion protein containing Asia tospoviral common epitope consists of an amino acid sequence as set forth in SEQ ID NO. 1, and a predetermined protein fragment connecting to the Asia tospoviral common epitope. The above eukaryotic expression system is useful for monitoring the interaction between proteins via use of a specific peptide to tag the predetermined protein and demonstrates high sensitivity and stability. | 05-07-2015 |
20150125850 | NOVEL RAPID AND HIGHLY SENSITIVE CELL BASED SYSTEM FOR THE DETECTION AND CHARACTERIZATION OF HIV - The invention pertains to a novel cell line, an HIV tat-rev dependent GFP-Gaussia luciferase Reporter cell line, known henceforth as the GGR cell line, that detects pseudotype and replication competent HIV (cloned or uncloned isolates, in cell media or human serum) rapidly and with high sensitivity. This GGR cell line provides an improved method of characterizing the entry phenotype of HIV envelope genes, and detecting and examining primary HIV samples in the context of laboratory research, clinical trial monitoring, and medical diagnostics. Examples include, but are not limited to, determining the functional HIV viral load, responsiveness to treatment, characterization of viral co-receptor usage (testing for viral co-receptor usage, i.e., CCR5 vs CXCR4, as required prior to prescribing FDA-approved CCR5 inhibitors), and characterization of other viral or drug resistance phenotypic properties to guide treatment. | 05-07-2015 |
20150125851 | OPTICAL SENSOR AND MANUFACTURING METHOD THEREOF, AND DETECTION METHOD UTILIZING SAME - An optical sensor is configured to be used with trappers specifically bound to an object substance to detect whether the object substance exists or not in a specimen. The optical sensor includes a first metal layer made of gold having a lower surface and an upper surface which is configured to have an electromagnetic wave supplied thereto, and a second layer made of gold having an upper surface facing the lower surface of the first metal layer. A hollow area configured to be filled with the specimen is provided between the first metal layer and the second metal layer. The trappers are physically bonded to at least one of a lower side of the first metal layer and an upper side of the second metal layer. The thickness of the first metal layer is not smaller than 5 nm and not larger than 30 nm. The optical sensor has a small size and a simple structure. | 05-07-2015 |
20150125852 | METHOD FOR EVALUATION OF VIABILITY OF VIRUSES WITH LYMPHOTROPISM PROPERTIES - Methods and techniques to increase the reliability of detecting virus infections, particularly lymphotropism, to eliminate false negative reactions in testing blood for the presence of lymphotropic viruses during enzyme immunoassay (EIA) and polymerase chain reaction (PCR) testing, and to better detect viruses with lymphotropism in biological materials having a concentration of virus particles lower than the sensitivity threshold of existing EIA and PCR methods, thereby making the techniques of the present invention more reliable. | 05-07-2015 |
20150125853 | TREND ANALYSIS AND STATISTICAL PROCESS CONTROL USING MULTITARGETED SCREENING ASSAYS - Aspects of the present invention provide novel multi-targeted microbiological screening and monitoring methods having substantial utility for monitoring and control of microbial growth and contaminants, microbiological processes, predictive microbiology, and for exposure and risk assessment. Microbial markers shared by both target and index microbes are used in novel methods for microbial monitoring, monitoring of microbial performance potential, trend analysis, and statistical process control (SPC) in processes or systems that are receptive to a plurality of genetically distinct microbes. | 05-07-2015 |
20150132740 | LANTHANUM-BASED CONCENTRATION AGENTS FOR MICROORGANISMS - A concentration agent for microorganisms is provided that contains both lanthanum and carbonate. Additionally, articles that include the concentration agent and methods of concentrating a microorganism using the concentration agent are provided. | 05-14-2015 |
20150132741 | EXTRACTION CONTROL FOR DNA - The present invention relates to compounds for use in the control of extraction procedures, particular in connection with nucleic acid material for use in PCR and more preferably real time PCR (quantitative PCR), The extraction control according to the present invention is based on non-pathogenic bacterial material which can be produced at low cost, in large quantities and which has good stability. | 05-14-2015 |
20150132742 | Microfluidic Devices Formed From Hydrophobic Paper - Microfluidic devices fabricated from paper that has been covalently modified to increase its hydrophobicity, as well as methods of making and using thereof are provided herein. The devices are typically small, portable, flexible, and both easy and inexpensive to fabricate. Microfluidic devices contain a network of microfluidic components, including microfluidic channels, microfluidic chambers, microwells, or combinations thereof, designed to carry, store, mix, react, and/or analyze liquid samples. The microfluidic channels may be open channels, closed channels, or combinations thereof. The microfluidic devices may be used to detect and/or quantify an analyte, such as a small molecules, proteins, lipids polysaccharides, nucleic acids, prokaryotic cells, eukaryotic cells, particles, viruses, metal ions, and combinations thereof. | 05-14-2015 |
20150132743 | PROXIMITY ASSAYS FOR DETECTING NUCLEIC ACIDS AND PROTEINS IN A SINGLE CELL - Methods and reagents for detection and analysis of nucleic acids and proteins using proximity extension assays. | 05-14-2015 |
20150132744 | METHODS OF DETECTING CELLS LATENTLY INFECTED WITH HIV - Disclosed are methods of detecting and isolating CD4 | 05-14-2015 |
20150140547 | METHODS AND COMPOSITIONS FOR ASSESSING COPY NUMBER OF TARGET POLYNECLEOTIDES - The present invention relates to methods and compositions for assessing copy number of a target polynucleotide in a sample, methods and compositions for establishing a standard curve for a target polynucleotide, and the uses of the methods and compositions for detecting a pathogen, e.g., human papillomavirus (HPV). | 05-21-2015 |
20150140548 | EXTRACTION CONTROL FOR RNA - The present invention relates to compounds for use in the control of extraction procedures, in particular in connection with nucleic acid material for use in PCR, in particular RT-PCR and more preferably real-time RT-PCR (quantitative PGR). The extraction control according to the present invention is based on plant virus material which can be produced at low cost, in large quantities and which has good stability. | 05-21-2015 |
20150140549 | Polymersome Encapsulation of Hydrophobic Fluorescent Polymers - Described herein are aqueous soluble polymersomes that encapsulate one or more hydrophobic fluorescent polymers and methods of their preparation and use. | 05-21-2015 |
20150140550 | Methods for Diagnosing Pervasive Development Disorders, Dysautonomia and Other Neurological Conditions - Methods for aiding in the diagnosis of disorders including, but not limited to, PDDs (Pervasive Development Disorders), Dysautonomic disorders, Parkinson's disease and SIDS (Sudden Infant Death Syndrome). In one aspect, a diagnosis method comprises analyzing a stool sample of an individual for the presence of a biological marker (or marker compound) comprising one or more pathogens, which provides an indication of whether the invidual has, or can develop, a disorder including, but not limited to, a PDD, Dysautonomia, Parkinsons disease and SIDS. Preferably, the presence of one or more pathogens is determined using a stool immunoassay to determine the presence of antigens in a stool sample, wherein such antigens are associated with one or more pathogens including, but not limited to, | 05-21-2015 |
20150140551 | DEVICE AND METHOD FOR INVESTIGATING ONE OR A PLURALITY OF PHASE OBJECTS - A method for investigating one or a plurality of phase objects is described, in which a grid made up of elements is used, which is illuminated with light of a light source, the coherence length of which is larger than the average spacing of adjacent elements of the grid. A diffraction image of the illuminating light scattered on the grid is generated, whereby the one or the plurality of phase objects are placed in the light path between the light source and the grid and/or in the light path of the illuminating light scattered on the grid. At least a part of the diffraction image is detected by an optical sensor directly or after interaction with further optical components and converted into a signal. The signal is analysed further in order to ascertain information relating to the one or plurality of phase objects therefrom. A corresponding device is likewise described. | 05-21-2015 |
20150140552 | QUANTITATIVE ANALYTE ASSAY DEVICE AND METHOD - The present invention related to a quantitative assay device and a method for the determination of an analyte, based on a test strip, which contains a porous test membrane allowing for capillary flow of the analyte and complexes of the analyte, a porous upstream membrane in fluid connection with the test membrane and a porous downstream membrane in fluid connection with the test membrane, wherein the test membrane contains two bands having deposited on there high and low concentrations of different calibrator agents and a test band capable of reacting with conjugated analyte complexes giving rise to a measurable signal. | 05-21-2015 |
20150147746 | MEANS AND METHODS USING PARAMAGNETIC AGENTS FOR IN VITRO DIAGNOSTIC APPLICATIONS - A method of detecting a target biochemical molecular species or at least one property correlated with the occurrence of the biochemical molecular species in a sample whose main component is water. The method includes: obtaining a sample whose main component is water; providing Functionalized Paramagnetic Particles (FPP) including a paramagnetic core and a moiety configured to interact with the target biochemical molecular species or with molecules collectively reporting on a property of the target biochemical molecular species; contacting the FPP with the sample; exposing the sample to an applied magnetic field; measuring a change in a nuclear relaxation property of the sample; and correlating the change to the presence of the biochemical molecular species in the sample or to at least one property correlated with the occurrence of the biochemical molecular species in the sample. | 05-28-2015 |
20150147747 | COMPOSITION COMPRISING OF A CONDUCTING POLYMER FOR DETECTING, CAPTURING, RELEASING, AND COLLECTING CELL - The present invention relates to a composition in which a conducting polymer is doped with a dopant, and a diagnostic apparatus, and more particularly, to a composition which is used to diagnose a disease and detect a biomaterial and also used for qualification and diagnosis by effectively and non-destructively collecting a captured biomaterial. Further, the composition can maximize capturing efficiency by being attached to a surface of a nano-structured scaffold and can be used as an ultrahigh-sensitive sensor using various linked bodies. | 05-28-2015 |
20150147748 | IN SITU DETECTION OF EARLY STAGES AND LATE STAGES HPV INFECTION - Embodiments of the invention provide methods, monoclonal antibodies, polyclonal antibodies, assays, and kits for detecting HPV infection and HPV related cancer diagnosis, including infection by various HPV genotypes, early and/or late stage HPV-associated or HPV-specific cancers. The anti-HPV antibodies are used in performing immunological assays on clinical samples. Various immunological assays and kits for detecting HPV infection, cervical cancer, other HPV related cancers, early stage precancerous lesions as well as late stage cancer progression are also provided. | 05-28-2015 |
20150147749 | Compositions and Methods Including Recombinant B Lymphocyte Cell Line Including an Exogenously Incorporated Nucleic Acid Expressing an Exogenous Membrane Immunoglobulin Reactive to a First Antigen and Including an Endogenous Gene Expressing an Endogenous Secreted Immunoglobulin Reactive to a Second Antigen - Compositions and methods are disclosed herein for producing one or more immunoglobulins in an isolated B lymphocyte cell line. An isolated cell line includes an isolated B lymphocyte cell line capable of expressing at least one exogenously incorporated membrane immunoglobulin reactive to a first antigen and at least one endogenous secreted immunoglobulin reactive to a second antigen. | 05-28-2015 |
20150147750 | NEW IRIDIUM-BASED COMPLEXES FOR ECL - The present invention relates to novel iridium-based Ir(III) luminescent complexes, conjugates comprising these complexes as a label and their application, e.g. in the electrochemiluminescence based detection of an analyte. | 05-28-2015 |
20150147751 | NEW IRIDIUM-BASED COMPLEXES FOR ECL - The present invention relates to novel iridium-based Ir(III) luminescent complexes, conjugates comprising these complexes as a label and their application, e.g. in the electrochemiluminescence based detection of an analyte. | 05-28-2015 |
20150147752 | NEW IRIDIUM-BASED COMPLEXES FOR ECL - The present invention relates to novel iridium-based Ir(III) luminescent complexes, conjugates comprising these complexes as a label and their application, e.g. in the electrochemiluminescence based detection of an analyte. | 05-28-2015 |
20150292043 | NUCLEIC ACID SEQUENCES THAT CAN BE USED AS PRIMERS AND PROBES IN THE AMPLIFICATION AND DETECTION OF ALL SUBTYPES OF HIV-1 - The present invention is related to nucleic acid sequences that can be used in the field of virus diagnostics, more specifically the diagnosis of infections with the AIDS causing Human Immuno-deficiency Virus (HIV). With the present invention nucleotide sequences are provided that can be used as primers and probes in the amplification and detection of HIV-1 nucleic acid. The oligonucleotide sequences provided with the present invention are located in the LTR part of the HIV viral genome. It has been found that, by using the sequences of the present invention in methods for the amplification and detection of nucleic acid a sensitive and specific detection of HIV-1 can be obtained. The benefit of the sequences of the present invention primarily resides in the fact that, with the aid of primers and probes comprising the sequences according to the invention the nucleic acid of all presently known subtypes of HIV-1 can be detected with high accuracy and sensitivity. So far no primer pairs or hybridization probes have been developed that would allow the detection of such a broad range of HIV-1 variants. The oligonucleotide sequences according to the present invention are especially useful in methods for the amplification of nucleic acid. | 10-15-2015 |
20150299813 | METHODS FOR DETECTING PATHOGEN IN COLDWATER FISH - The present invention relates to a method for detecting a pathogen in coldwater fish. In addition, the present invention also relates to pairs of oligonucleotides for detecting pathogens in coldwater fish. | 10-22-2015 |
20150301047 | METHOD FOR RECOGNITION OF A PRESENCE OF AN INFECTION WITH CMV - A method for recognition of a presence of an infection with CMV in a patient by—determination of a level of CD28neg/CD27neg CD4 T-cells in a whole blood sample obtained from the patient;—comparing the level of CD28neg/CD27neg CD4 T-cells with a level of a control group not infected with CMV; and—defining the presence of the infection with CMV, if the level of CD28neg/CD27neg CD4 T-cells in the patient is significantly higher than the level of CD28neg/CD27neg CD4 T-cells of the control group. | 10-22-2015 |
20150307926 | DETECTION UNITS AND METHODS FOR DETECTING A TARGET ANALYTE - The present application relates to detection units and methods for detecting one or more target analytes in a sample. The detection unit provides a first and second surface connected by a filament which is capable of binding the target analyte in the sample. Double-stranded DNA molecules are provided having a continuous strand and a discontinuous strand, and an active segment that is designed to hybridize to a target nucleic acid of interest, where the continuous strand has between 0 and 100 unpaired nucleotides in the active segment and the discontinuous strand has between 5 and 100 unpaired nucleotides at its 3′ end and/or its 5′ end. The unpaired nucleotides in the continuous strand can form a secondary structure, such as a loop. The methods provide for the detection of the target analyte through the generation of a detectable signal, such as supercoiling, following the binding of the target analyte to the filament and can be used to detect nucleic acids of interest including single nucleotide polymorphisms or somatic mutations. | 10-29-2015 |
20150307951 | COMPOSITIONS AND METHODS FOR DETECTING HUMAN PAPILLOMAVIRUS NUCLEIC ACID - Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of a human papillomavirus (HPV) nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits. | 10-29-2015 |
20150309018 | SYSTEM AND METHOD FOR ELECTRONIC BIOLOGICAL SAMPLE ANALYSIS - A biological sample analysis device includes a casing that encloses a biological sample delivery system hydraulically coupled to a sensor, wherein the sensor includes a plurality of Graphene transistors and each transistor covalently bonds with a biomarker causing the electrical properties of the transistor to measurably change when the biomarker is exposed to corresponding antibodies within an infected biological sample. | 10-29-2015 |
20150315635 | COMPOSITIONS AND METHODS FOR QUANTIFYING A NUCLEIC ACID SEQUENCE IN A SAMPLE - The present invention features compositions and methods for quantifying detection of a target oligonucleotide in a sample in real time. These methods are compatible with target oligonucleotides amplified using a NEAR reaction. | 11-05-2015 |
20150316543 | METHODS AND REAGENTS FOR THE DETECTION OF BIOMOLECULES USING LUMINESCENCE - The present invention relates to luminescent complexes comprising a charged transfer complex of metal atomic quantum clusters (AQCs) of at least two different sizes and a biotin-binding molecule, preferably streptavidin, and the use thereof for the detection of biotinylated compounds. The invention also relates to the use of conjugates comprising a charged transfer complex of AQCs and a biomolecule and the use thereof for the detection of binding partners of the biomolecule in a sample based on the luminescent properties of the AQCs nanosystems. | 11-05-2015 |
20150323534 | METHODS AND COMPOSITIONS FOR ANALYTE DETECTION - The present invention is directed to methods and apparatus for detection of one or more analytes. Analytes include agents or components of infectious agents such as pathogenic virus, as well as enzymes, proteins and biomarkers. | 11-12-2015 |
20150330977 | MULTISENSING PLATFORM USING NANOPOROUS CANTILEVER SENSOR - Provided is a nanoporous cantilever including a substrate in a shape of a plate, a tip formed at a front end of the substrate, and a nanoporous structure formed on the tip. Due to the nanoporous structure including nanopores and nanochannels formed on the tip, the nanoporous cantilever greatly increases in detection sensitivity for a material to be detected when compared to a cantilever according to a related art, and may obtain a surface-enhanced Raman signal and thus achieve discrimination of a molecule to be detected. | 11-19-2015 |
20150330980 | METHODS AND KITS FOR THE DETECTION OF VIRAL INFECTIONS - The invention relates to an improved assay for detecting antibodies in a tissue sample from individuals who test seronegative by conventional assay techniques, thus aiding in the diagnosis of possible pathogenic infections. Specifically, the invention relates to improved assay methods and kits that enable efficient detection of antibodies against a viral infection. | 11-19-2015 |
20150330982 | PRETREATMENT METHOD OF SAMPLE FOR DETECTING HBS ANTIGEN AND USE THEREOF - The present invention relates to a pretreatment method of a sample for detecting HBs antigen, which is a surface antigen of the hepatitis B virus, and a method for detecting HBs antigen utilizing the pretreatment method. The present invention also relates to a pretreatment reagent kit for detecting HBs antigen. | 11-19-2015 |
20150337356 | METHOD FOR IN VITRO DETECTION AND MONITORING OF A DISEASE BY MEASURING DISEASE-ASSOCIATED PROTEASE ACTIVITY IN EXTRACELLULAR VESICLES - The present invention relates to a method for in vitro detection and/or monitoring of a disease in a sample, based on measurement of enzymatic activity of proteases activated and secreted upon disease development, to modified peptides used for the enzymatic detection of the proteases, the use of the peptides, a kit comprising such peptides and the use of ADAM-protease activity as a surrogate marker for disease burden and activity in infectious, inflammatory, and malignant diseases, such as HIV infection and melanoma. | 11-26-2015 |
20150337362 | METHOD FOR THE SPECIFIC ISOLATION OF NUCLEIC ACIDS OF INTEREST - A method and diagnostic kit for selective isolation of microorganisms of interest and/or nucleic acids of interest including: a) bringing a liquid biological sample into contact with a saponin formulation to destabilize untargeted elements within the liquid biological sample, b) inducing osmotic shock of the untargeted elements to specifically lyse the untargeted elements, c) adding a solution of at least one enzyme capable of lysing free nucleic acids derived from the untargeted elements lysed in solution in the sample, and d) selectively obtaining the microorganisms of interest or the nucleic acids of interest. A precipitant of the unlysed microorganisms of interest may be optionally added in solution. The microorganisms of interest have cell membranes or capsids that do not contain cholesterol. The untargeted elements may include untargeted cells having cell membranes containing cholesterol, and optionally viruses with envelopes containing cholesterol, mycoplasmas containing cholesterol, and cell debris. | 11-26-2015 |
20150338324 | Methods and Device for Trapping, Moving and Sorting Particles Contained in a Fluid - The invention relates to a method for trapping particles contained in a fluid including at least the steps of generating a coherent light beam, diffusing the coherent light beam using a passive diffusive element to yield a diffused beam having a field of optical speckles, and causing the diffused beam to interact with a plurality of particles contained in a fluid. | 11-26-2015 |
20150338345 | One Dimensional Photonic Crystals for Enhanced Fluorescence Based Sensing, Imaging and Assays - Techniques for enhanced fluorescence include a functionalized substrate for a target optical frequency comprising a one dimensional photonic crystal that is functionalized with a bioactive target molecule that has an affinity for a particular analytic. The one dimensional photonic crystal includes a plurality of dielectric layers including a plurality of high index of refraction layers alternating with a plurality of low index of refraction layers. The thickness of each layer is within a factor of four of a wavelength of the optical frequency in the layer. For emissions from a fluorophore bound to the target molecule and excited by incident light, there is an emission intensity maximum centered at an angle independent of the direction of the incident light. | 11-26-2015 |
20150344870 | NUCLEIC ACID EXTRACTION - A method and device for extracting nucleic acids from a biological sample is described. The device includes a substrate, such as a cellulose filter, functionalised with a biocidal agent having multiple functional groups including a binding moiety, which is involved in binding the agent to the substrate; a hydrophobic moiety; and a charged moiety. The various functional groups serve to bind the agent to the substrate, weaken or lyse the cell wall or membrane of the sample, and retain nucleic acids on the substrate. A preferred biocidal agent is a silylated quaternary ammonium compound (SiQAC), for example 3-(trimethoxysilyl) propyldimethyloctadecyl ammonium chloride. | 12-03-2015 |
20150344930 | CODON-OPTIMIZED RECOMBINANT PHAGE AND METHODS OF USING SAME - Composition and methods for the detection of one or more target microbe(s) are provided. Compositions of the disclosure include at least one recombinant phage capable of infecting a target microbe, said phage comprising at least a capsid protein sequence, a ribosome binding site, and a codon-optimized marker. Compositions of the disclosure may further include an aqueous solution that enhances the ability to detect marker expression upon phage infection of the target microbe. In some embodiments the target microbe include is | 12-03-2015 |
20150346215 | FILOVIRUS FUSION PROTEINS AND THEIR USES - This invention provides fusion proteins comprising a Filovirus glycoprotein segment and an immunoglobulin polypeptide segment. The fusion proteins are useful in immunogenic compositions to protect against infections by Filoviruses, such as Ebola virus, in both humans and non-human animals. The fusion proteins are also useful in diagnostic assays to detect Filovirus infections. | 12-03-2015 |
20150351372 | HUMANIZED DIPEPTIDYL PEPTIDASE IV (DPP4) ANIMALS - Non-human animals comprising a human or humanized DPP4 nucleic acid sequence are provided. Non-human animals that comprise a replacement of the endogenous Dpp4 gene with a human or humanized DPP4 gene, or non-human animals comprising a human or humanized DPP4 gene in addition to the endogenous Dpp4 gene are described. Non- human animals comprising a human or humanized DPP4 gene under control of human or non-human DPP4 regulatory elements is also provided, including non-human animals that have a replacement of non-human Dpp4-encoding sequence with human DPP4-encoding sequence at an endogenous non-human Dpp4 locus. Non-human animals comprising human or humanized DPP4 gene sequences, wherein the non-human animals are rodents, e.g., mice or rats, are provided. Methods for making and using the non-human animals are described. | 12-10-2015 |
20150353351 | Compact Fluid Analysis Device and Method to Fabricate - The present disclosure relates to a device for analyzing a fluid sample. In one aspect, the device includes a fluidic substrate that comprises a micro-fluidic component embedded in the fluidic substrate configured to propagate a fluid sample via capillary force through the device and a means for providing a fluid sample connected to the micro-fluidic component. The device also includes a lid attached to the fluidic substrate at least partly covering the fluidic substrate and at least partly closing the micro-fluidic component. The fluidic substrate may be a silicon fluidic substrate and the lid may be a CMOS chip. In another aspect, embodiments of the present disclosure relate to a method for fabricating such a device, and the method may include providing a fluidic substrate, providing a lid, and attaching, through a CMOS compatible bonding process, the fluidic substrate to the lid to close the fluidic substrate at least partly. | 12-10-2015 |
20150353900 | INFLUENZA VIRUS REASSORTMENT - Improved methods for the production of reassortant influenza viruses are provided. | 12-10-2015 |
20150353996 | Reusable Long Period Microfiber Grating for detection of DNA Hybridization - A label free, reusable and high sensitivity viral fiber sensor is provided in the present invention. The label free, high sensitivity and reusability are the advantages of this sensor. Long Period Microfiber Grating (LPMFG) is used for the sensing device. It allows optically detecting the change in refractive index at the grating surface with an extra high sensitivity. This provides an optical detection method to monitor DNA Hybridization. The single stranded DNA (ssDNA) probe is immobilized onto the LPMFG's surface for hybridizing with a DNA sample in order to identify the viral strain in the sample. This LPMFG-based viral sensor functions by inducing a refractive index change on the grating surface through the bio-molecule binding between the target viral ssDNA and the immobilized probe ssDNA. Regeneration of a surface-immobilized probe without a significant loss of hybridization activity retains at least 10 successive assays without any significant loss of performance (less than 10% decrease). | 12-10-2015 |
20150354016 | COMPOSITIONS, METHODS AND KITS TO DETECT HERPES SIMPLEX VIRUS NUCLEIC ACIDS - The disclosed invention is related to methods, compositions, kits and isolated nucleic acid sequences for targeting Herpes Simplex Virus (HSV) nucleic acid (e.g., HSV-1 and/or HSV-2 nucleic acid). Compositions include amplification oligomers, detection probe oligomers and/or target capture oligomers. Kits and methods comprise at least one of these oligomers. | 12-10-2015 |
20150355178 | SYSTEM AND METHOD FOR IMPROVING BIOMARKER ASSAY - The present disclosure pertains to detection of biomarkers in a sample. More particularly, the disclosure relates to methods for treating the sample to liberate certain analytes prior to the assay. Composition for disrupting the HIV virus and antibody-antigen complex to release p24 antigen is also disclosed. The disclosed methods and compositions are compatible with existing HIV antigen/antibody combination assays and improve the sensitivity of such assays. | 12-10-2015 |
20150361417 | ASSAY FOR DETECTION OF JC VIRUS DNA - In one aspect, the disclosure provides methods for isolating nucleic acid from a Cerebrospinal Fluid (CSF) sample. In one aspect, the disclosure provides methods for determining the amount of JC virus DNA in a sample. | 12-17-2015 |
20150361419 | MICROFLUIDIC CHIP FOR EXTRACTING NUCLEIC ACIDS, DEVICE FOR EXTRACTING NUCLEIC ACIDS COMPRISING SAME, AND METHOD FOR EXTRACTING NUCLEIC ACIDS USING SAME - The present invention relates to a microfluidic chip for extracting nucleic acids, a nucleic acid extraction device having the same, and a nucleic acid extraction method using the same that can provide micro-miniaturization and ultra high speed, while maintaining and/or improving reliable nucleic acid extraction efficiencies, unlike the existing nucleic acid extraction device and method. | 12-17-2015 |
20150361482 | PRIMERS AND PROBES FOR DETECTING HUMAN PAPILLOMAVIRUS AND HUMAN BETA GLOBIN SEQUENCES IN TEST SAMPLES - The present invention relates to primers, probes, primer sets, primer and probe sets, methods and kits for detecting human papillomaviruses, human beta globin sequences and human papillomaviruses and human beta globin sequences in a test sample. | 12-17-2015 |
20150361484 | METHODS AND MATERIALS FOR DETECTING CONTAMINATED FOOD PRODUCTS - This document provides methods and materials for detecting contaminated food products. For example, methods and materials for using an enzymatic amplification cascade of restriction endonucleases to detect nucleic acid of a microorganism or virus (e.g., a pathogen) within a sample (e.g., food product sample) being tested, thereby assessing a food product for possible contamination are provided. | 12-17-2015 |
20150361485 | METHODS AND MATERIALS FOR DETECTING VIRAL OR MICROBIAL INFECTIONS - This document provides methods and materials for detecting target nucleic acid. For example, methods and materials for detecting the presence or absence of target nucleic acid, methods and materials for detecting the amount of target nucleic acid present within a sample, kits for detecting the presence or absence of target nucleic acid, kits for detecting the amount of target nucleic acid present within a sample, and methods for making such kits are provided. | 12-17-2015 |
20150361505 | Detection of hepatitis B virus (HBV) DNA and methylated HBV DNA in urine of patients with HBV-associated hepatocellular carcinoma - This application relates to a DNA marker for HBV-HCC detection and the methods, kits for quantitatively measuring the amount of HBV DNA and bisulfite treated HBV DNA, and methylated HBV DNA, and the aberrant methylation of the HBV genome for the used in the chronic HBV infected populations. Detection of the presence or absence of HCC, with elevated methylation levels in the one or more regions of DNA of the mammals as compared to the level of methylation in the one or more regions of DNA in the one or more control body fluids or tissues indicating the presence of the cancer, and the absence of elevated methylation levels indicating the absence of HCC. | 12-17-2015 |
20150361511 | FREEZE-DRIED COMPOSITION - The invention relates to the use of a polysaccharide having at least four saccharide units, such as stachyose, as a glass-forming agent for the freeze-drying of a reaction mixture comprising an enzyme. In particular, the enzyme is a polymerase useful in a nucleic acid amplification reaction such as a Polymerase Chain Reaction. Compositions comprising such polysaccharides as well as methods for preparing them, kits containing them and methods for using them form further aspects of the invention. | 12-17-2015 |
20150368717 | SYSTEMS AND METHODS FOR MULTI-ANALYSIS - Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample. | 12-24-2015 |
20150368727 | NUCLEIC ACID AMPLIFICATION CONTROLS - The present invention discloses positive control material for nucleic acid amplification based detection of microorganisms in biological samples. The control material comprises purified microorganism that is rendered non-infectious but is amenable to nucleic acid amplification. Also disclosed is a process for making and using the control material. | 12-24-2015 |
20150368728 | DENGUE VIRUS ASSAY - Nucleic acid assays for detecting nucleic acids of Dengue virus serotypes 1-4. | 12-24-2015 |
20150369798 | Apparatus for Improved Disease Detection - An apparatus for detecting a disease in a biological subject comprises a delivery system and at least two sub-equipment units which are combined or integrated in the apparatus, wherein the delivery system is capable of delivering the biological subject to at least one of the sub-equipment units and each sub-equipment unit is capable of detecting at least one property of the biological subject. Methods for detecting a disease with the apparatus are also provided. | 12-24-2015 |
20150369806 | DETECTION OF VIRAL DISEASES USING A BIOCHIP THAT CONTAINS GOLD NANOPARTICLES - A sensor for detecting molecular interactions between a target and a binding domain by electrochemical impedance spectroscopy. The sensor includes an anodic aluminum oxide barrier layer having a gold-coated array of regularly spaced nano-hemispheres and gold nanoparticles coated with a binding domain attached thereto. Also provided are methods for producing the sensor and for using the sensor to detect the presence of a virus in a sample. | 12-24-2015 |
20150376689 | DYNAMIC FLUX NUCLEIC ACID SEQUENCE AMPLIFICATION - Provided herein are dynamic flux nucleic acid sequence amplification methods. The dynamic flux nucleic acid sequence amplification methods described herein are capable of amplifying nucleic acid sequences within a narrow temperature range. In some aspects, the disclosure provides for real-time dynamic flux nucleic acid sequence amplification methods. | 12-31-2015 |
20150376721 | Direct Quantitative PCR Absent Minor Groove Binders - Disclosed herein are methods, compositions and kits for the quantification of a nucleic acid target present on a solid support. This entails quantitative real-time polymerase chain reaction wherein minor groove binders are excluded. | 12-31-2015 |
20150376722 | COMPOSITIONS AND METHODS FOR DETECTING HUMAN PEGIVIRUS 2 (HPgV-2) - Provided herein are compositions, methods, and kits for detecting human Pegivirus 2 (HPgV-2). In certain embodiments, provided herein are HPgV-2 specific nucleic acid probes and primers, and methods for detecting HPgV-2 nucleic acid. In other embodiments, provided herein are HPgV-2 immunogenic composition compositions, methods of treating a subject with immunogenic HPgV-2 peptides, and methods of detecting HPgV-2 subject antibodies in a sample. | 12-31-2015 |
20160002355 | IDIOTYPIC ANTIBODIES AND USES THEREOF - The invention provides anti-idiotypic HCMV antibodies as well as methods of using the same. | 01-07-2016 |
20160002700 | Filter and Blower Geometry for Particle Sampler - The invention provides devices and methods for sampling, detecting and/or characterizing particles. Devices and methods of the invention, including particle samplers, impactors and counters, include a filter component for removing particles in the exhaust flow of the device, for example, to eliminate or minimize the potential for the device itself to provide source of particles in an environment undergoing particle monitoring. This aspect of the present devices and methods is particularly useful for monitoring particles in manufacturing environments requiring low levels of particles, such as cleanroom environments for electronics manufacturing and aseptic environments for manufacturing pharmaceutical and biological products. | 01-07-2016 |
20160002743 | HIGH RESOLUTION MELTING ANALYSIS ASSAY FOR THE DETECTION OF VIRAL DNA - In one aspect, the disclosure provides methods, kits and compositions for determining the presence of a JC virus mutant in a sample. | 01-07-2016 |
20160003744 | Plasmonic Nanocavity Array Sensors for Analyte Detection Enhancement and Methods for Making and Using of the Same - This disclosure provides, among other things, a nanosensor for sensing an analyte. In some embodiments the nanosensor comprises (a) a substrate; (b) a signal amplification layer comprising: (i) a substantially continuous metallic backplane on the substrate; (ii) one or a plurality of pillars extending from the metallic backplane or from the substrate through holes in the backplane; and (iii) a metallic disk on top of the pillar, wherein at least one portion of the edge of the disk is separated from the metallic backplane; and (c) a capture agent that specifically binds to the analyte, wherein the capture agent is linked to the surface of the signal amplification layer; wherein the nanosensor amplifies a light signal from an analyte, when the analyte is bound to the capture agent. Methods for fabricating the nanosensor and methods for using the nanosensor are also provided. | 01-07-2016 |
20160003823 | SYSTEMS AND METHODS FOR MULTI-ANALYSIS - Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample. | 01-07-2016 |
20160007579 | HUMANIZED DIPEPTIDYL PEPTIDASE IV (DPP4) ANIMALS | 01-14-2016 |
20160017291 | MICROCARRIER PERFUSION CULTURING METHODS AND USES THEREOF - Provided herein are methods of culturing a mammalian cell and various methods that utilize these culturing methods. | 01-21-2016 |
20160018344 | SENSOR AND METHOD OF DETECTING AN ANALYTE USING 19F NMR - A sensor including a fluorinated receptor can be used to identify an analyte through shift in | 01-21-2016 |
20160024548 | DNA POLYMERASES AND RELATED METHODS - Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. The mutant polymerases overcome the inhibitory effects of a variety of polymerase and reverse transcriptase inhibitors. Therefore, the mutant polymerases are useful in a variety of disclosed methods in the presence of such inhibitors. | 01-28-2016 |
20160024602 | Methods of Evaluating a Cellular Sample for Latent Cellular Replication Competent HIV-1, and Compositions and Kits for Use in Practicing the Same - Methods of evaluating a cellular sample for latent cellular replication competent HIV-1 are provided. Aspects of the methods include contacting a cellular sample with an HIV-1 inducing compound to produce an activated cellular sample; and assessing plasma viral load in the activated cellular sample to evaluate the cellular sample for latent cellular replication competent HIV-1. Also provided are devices and kits that find use in practicing the methods. | 01-28-2016 |
20160025634 | Composite Nanoparticle Structures for Chemical and Biological Sensing - Described herein is a nanoparticle that enhances the interaction of the nanoparticle and/or a molecule/material deposited on the surface of the nanoparticle with light, comprising a pair of stacked metallic disks separated by a non-metallic spacer, wherein: (a) the dimensions of the disks and spacer are smaller than the wavelength of the light; and (b) the nanoparticle enhance the light interaction at least three times greater than that an individual metallic disk. Methods for making the nanoparticle and methods for using the nanoparticle in a variety of assays are also provided. | 01-28-2016 |
20160025639 | POINT OF CARE ANALYTICAL PROCESSING SYSTEM - A point of care testing system includes a reader having an incubator disposed within a reader housing, the incubator having a rotor supported for rotation and having a plurality of circumferentially disposed slots. A drive mechanism is configured to rotate the rotor about a center axis A plurality of analytical test elements are sized for fitting in the slots of the incubator either manually or on demand. Each analytical test element commonly includes a support within a cartridge. The support retains at least one of a dry chemistry chip comprising a porous spreading layer disposed in stacked relation with at least one reagent layer or a lateral flow assay device wherein the plurality of test elements can assume a common form factor with multiplexed capability, and in which cartridges are preferably gated to enable random access processing. | 01-28-2016 |
20160025706 | METHOD OF MEASURING HUMAN CYP3A INDUCIBILITY - A method for measuring human CYP3A inducibility upon administration of a test drug, characterized in that a non-human animal to which a test drug is administered or a population of human cells cultured in a medium containing a test drug is infected with viruses (A) and (B); virus (A) being an adenovirus which is used as a vector and engineered by incorporating thereto a detectable reporter gene and at least 3 human PXR binding regions falling within an untranslated region of a human CYP3A gene, and virus (B) being an adenovirus which is used as a vector and engineered by incorporating thereto a human PXR cDNA; and subsequently expression level of the reporter gene is determined in the non-human animal or the cultured human cells. | 01-28-2016 |
20160025724 | Materials and Methods for Assessing and Mapping Microbes and Microbial Biofilms on Wounds - The subject invention provides point-of-care assays for assessing the topographical distribution of microbial biofilm and/or specific microorganisms in wounds. | 01-28-2016 |
20160025727 | AUTOMATED IMAGING AND ANALYSIS OF THE HEMAGGLUTINATION INHIBITION ASSAY (HAI) - A system and method provide for high through put determination of agglutination states. The system includes a rotating table and multiple plate tilting stations. The system also includes one or more optical paths positioned to image entire plate arrays in tilted and/or untilted configurations. The system preferably includes image analysis software to analyze an image of an array of test wells and determine an agglutination state of each well based on the image analysis. | 01-28-2016 |
20160030952 | CENTRIFUGE DEVICE - A modular centrifuge device for separating fluid samples, including a housing having a modular power assembly mechanism for rotating samples with manual or electric power. A manual centrifuge device, including a housing having a power assembly mechanism for rotating samples with manual power, and a speed indicator for indicating if a predetermined speed has been reached and a time indicator for indicating if a predetermined or calculated time has been reached for rotating said samples operatively connected to the device. A method of centrifuging samples, by selecting a manual power mode or electric power mode on a centrifuge, rotating samples at a predetermined speed for a predetermined or calculated time, alerting a user that the predetermined speed and predetermined or calculated time have been achieved, and obtaining separated samples. | 02-04-2016 |
20160032345 | METHOD FOR PRODUCING SOLUBLE RECOMBINANT INTERFERON PROTEIN WITHOUT DENATURING - The present invention relates to the field of recombinant protein production in bacterial hosts. It further relates to extraction of soluble, active recombinant protein from an insoluble fraction without the use of denaturation and without the need for a refolding step. In particular, the present invention relates to a production process for obtaining high levels a soluble recombinant Type 1 interferon protein from a bacterial host. | 02-04-2016 |
20160032352 | Composition, Device, and Method for Biological Air Sampling - The present invention generally relates to air sampling of biological compounds. Specifically, the present invention relates to a device and method for sampling the ambient air for detecting microbial propagules, microbial propagules being any spore, vegetative cell, or virion of microbiological origin including all bacteria, fungi, viruses, protozoans, molds, slime molds, chlamydospores, hyphae, and cysts. | 02-04-2016 |
20160032365 | METHODS OF IDENTIFYING AND QUANTIFYING BACTERIA IN CHEWING GUM - The invention is directed to methods of extracting nucleic acids from microorganisms or mammalian cells adhered to polymers that are malleable within a living organism and particularly malleable in the oral cavity of the living organism. The invention also provides for method of detecting and quantitating microorganisms that adhere to malleable polymers, such as chewing gum. | 02-04-2016 |
20160032374 | Recombinase Polymerase Amplification - This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods may allow amplification of DNA up to hundreds of megabases in length. | 02-04-2016 |
20160032410 | DETECTION AND USE OF ANTIVIRAL RESISTANCE MUTATIONS - The present invention relates generally to viral variants exhibiting reduced sensitivity to particular agents and/or reduced interactivity with immunological reagents. More particularly, the present invention is directed to hepatitis B virus (HBV) variants exhibiting complete or partial resistance to nucleoside or nucleotide analogs and/or reduced interactivity with antibodies to viral surface components including reduced sensitivity to these antibodies. Vaccines and diagnostic assays are also contemplated herein. | 02-04-2016 |
20160032411 | SELECTIVE DETECTION OF HEPATITIS A, B, C, D, OR E VIRUSES OR COMBINATION THEREOF - Processes and compositions are provided for the detection of hepatitis viruses in a sample. Particular processes and compositions are provided for the selective detection of HDV. Also provided are processes and compositions for the simultaneous detection of two or more hepatitis viruses that for the first time provide rapid, reliable, and simple detection of any known hepatitis vims in a sample using a single set of reaction conditions. | 02-04-2016 |
20160032412 | COMPOSITIONS AND METHODS FOR DETECTING HEV NUCLEIC ACID - Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of Hepatitis E Virus (HEV) nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits. | 02-04-2016 |
20160033480 | Immunovir and Components, Immunovir A, B, C, D Utility and Useful Processes - A complete remedy for AIDS is difficult to obtain. As such, a useful process was designed to search for an anti-HIV | 02-04-2016 |
20160033494 | MAGNETIC NANOSENSOR COMPOSITIONS AND BIOANALYTICAL ASSAYS THEREFOR - Disclosed are magnetic nanosensors or transducers that permit measurement of a physical parameter in an analyte via magnetic reasonance measurements, in particular of non-agglomerative assays. More particularly, in certain embodiments, the invention relates to designs of nanoparticle reagents and responsive polymer coated magnetic nanoparticles. Additionally provided are methods of use of nanoparticle reagents and responsive polymer coated magnetic nanoparticles for the detection of a stimulus or an analyte with NMR detectors. | 02-04-2016 |
20160040156 | METHOD OF DNA/RNA EXTRACTION USING SILYATED QUATERNARY AMMONIUM COMPOUNDS (SIQAC) - There is described a method of extracting DNA and/or RNA from a cell or capsid, the method comprising contacting the cell or capsid with a composition comprising a quaternary ammonium compound including a silicon-containing functional group. The quaternary ammonium compound may be of general formula (I) or a derivative salt thereof wherein L is a linking group; each of R | 02-11-2016 |
20160041167 | METHODS AND KITS FOR THE DIAGNOSIS OF INFLUENZA - Some embodiments provided herein relate to combined assays. In some embodiments, an assay for identifying influenza type A or influenza type B is combined with an assay for determining the sensitivity of an influenza neuraminidase to an antiviral drug. | 02-11-2016 |
20160041172 | COMPOSITION FOR DETECTION OR DIAGNOSIS OF DISEASES CONTAINING TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR - The present disclosure relates to a composition or a kit that can be used for detection or diagnosis of various diseases. | 02-11-2016 |
20160047006 | DIAGNOSIS OF VIRAL INFECTIONS BY DETECTION OF GENOMIC AND INFECTIOUS VIRAL DNA BY MOLECULAR COMBING - A method for detecting in vitro the presence of a genome of a DNA virus or a viral derived DNA in an infected eukaryotic cell, tissue or biological fluid using Molecular Combing or other nucleic acid stretching methods together with probes, especially nucleic acid probes, having a special design. A method for monitoring in vitro the effects of anti-viral treatment by following the presence of genomic viral or viral derived DNA polynucleotides in a virus-infected cell, tissue or biological fluid. Detection of an infectious form of a virus using Molecular Combing and DNA hybridization. A kit comprising probes used to carry out these methods and a composition comprising the probes. | 02-18-2016 |
20160047802 | SIGNAL AMPLIFICATION MICROSPHERES, THEIR USE IN ONE-STEP AND MULTI-STEP ANALYTICAL AMPLIFICATION PROCEDURES AND METHODS FOR THEIR PRODUCTION - The present invention relates to microspheres comprising protein signal precursor molecules, or a carrier protein bonded to signal precursor molecules, wherein said signal precursor molecules are activatable to generate a detectable signal whilst remaining bonded to the carrier protein. Also disclosed is a method of making such microspheres comprising the steps of mixing protein molecules with a matrix former in solution; adding a reducing reagent to the mixture; removing the reducing reagent; and removing the matrix former to leave microspheres of protein molecules. Also disclosed are bioassay methods using the microspheres to provide signal amplification, including an amplification cycling procedure. | 02-18-2016 |
20160047803 | SELF-ASSEMBLED MONOLAYERS AND METHODS FOR USING THE SAME IN BIOSENSING APPLICATIONS - Cross-linked amphiphile constructs that form self-assembled monolayers (SAMs) on metal surfaces such as gold surfaces are disclosed. These new SAMs generate well packed and highly oriented monolayer films on gold surfaces. A method for using the SAMs in the fabrication of biomolecule sensors is also disclosed. | 02-18-2016 |
20160047804 | SIGNAL AMPLIFICATION IN PLASMONIC SPECIFIC-BINDING PARTNER ASSAYS - The present invention relates to analyte detection devices and methods of using such devices to detect minute quantities of a target analyte in a sample. In particular, the invention provides an analyte detection device comprising a plurality of composite metallic nanostructures conjugated to analyte binding partners and a surface containing a metallic nanolayer on which a plurality of capture molecules is immobilized. Methods of preparing composite nanostructures are also described. | 02-18-2016 |
20160047944 | METHODS AND APPARATUS FOR MONITORING INTERACTIONS BETWEEN PARTICLES AND MOLECULES USING NANOPHOTONIC TRAPPING - A method for characterizing an interaction between a first particle and a second particle is provided. The method includes the steps of: (i) providing an optical trap system including a photonics-based trap, a light source, and a camera; (ii) optically trapping, using the photonics-based trap, the first particle; (iii) obtaining a first measurement of a trap stiffness of the photonics-based trap; (iv) introducing the second particle to the optically trapped particle; (v) incubating the first and second particles under conditions suitable for an interaction between the first and second particles; (vi) obtaining a second measurement of the trap stiffness of the photonics-based trap after the incubation; and (vii) determining, using the first measurement of trap stiffness and the second measurement of trap stiffness, a property of the interaction between the first particle and the second particle. | 02-18-2016 |
20160053334 | AUTOMATED HIV-1 VIRAL LOAD TESTING PROCEDURE FOR DRIED SPOTS - The present invention provides novel and non-obvious improvements to dried blood spot testing for HIV-1 viral load useful for diagnosis and monitoring treatment progression. | 02-25-2016 |
20160054307 | LED ASSAY READER WITH TOUCHSCREEN CONTROL AND BARCODE SAMPLE ID - Assay devices, assay detection systems, and methods comprising same for analytical tests, medical assays, diagnostic tests, medical diagnosis, risk assessment, or quality control purposes are provided. These devices, systems, and methods are designed to be employed at the point of care, such as in emergency rooms, operating rooms, hospital laboratories and other clinical laboratories, doctor's offices, in the field, or in any situation in which a rapid and accurate result is desired. The systems and methods process samples, such as clinical, biological, or blood sample, and read data from colorimetric based biochemical assays to provide an indication of the presence or absence of a bacterial, fungal, or viral contaminants therein. The assay devices include an optical reader apparatus and barcode scanner for reading and matching the test results to identification information provided by the barcodes to facilitate ease of tracking compliant and noncompliant samples. | 02-25-2016 |
20160054321 | Methods For Detecting Human Metapneumovirus - The present invention discloses specific human metapneumovirus monoclonal antibodies. The antibody is at least two-fold less reactive with non-human metapneumoviruses including, but not limited to, respiratory viruses or avian metapneumoviruses. Further, the antibody is at least two-fold more reactive with a human metapneumovirus (i.e., for example, Type A or Type B) than with non-human metapneumoviruses including, but not limited to, respiratory viruses or avian metapneumoviruses. Consequently, these novel antibodies are useful as a clinical diagnostic agent, especially when using fresh nasopharengeal aspirates. The invention also contemplates numerous diagnostic platforms that together with the novel antibodies can support economical, fast, and highly selective detection and identification of clinical inoculum samples. | 02-25-2016 |
20160060717 | Amplification and Sequencing of Transrenal Nucleic Acids - The present invention provides highly sensitive methods used for diagnosing and monitoring various diseases and disorders by detecting and analyzing “ultra short” (20-50 base pair) nucleic acids obtained from bodily fluids. | 03-03-2016 |
20160061696 | METHOD OF REMOVING FLOATATION LIQUID - A method of removing a floatation liquid from between a microscope slide and a paraffin embedded biological specimen including position the microscope slide with the paraffin embedded biological specimen floated thereon onto a slide support element. The slide support element is rotated to cause the microscope slide and the paraffin embedded biological specimen to move in a way that causes the floatation liquid disposed between the microscope slide and the paraffin embedded biological specimen to be removed from between the microscope slide and the paraffin embedded biological specimen. | 03-03-2016 |
20160061830 | BACTERIOPHAGE-BASED BIOSENSOR FOR MICROBIAL DETECTION - A biosensor for detecting a microorganism in a sample includes a polymer matrix and an immobilized layer of bacteriophages on the surface of the polymer matrix, wherein interaction of the bacteriophage with the microorganism causes a detectable physicochemical change in the polymer matrix. A method of detecting a microorganism in a sample using the biosensor includes the steps of contacting the sample with the biosensor; allowing the bacteriophage to bind to the microorganism in the sample; and detecting a physicochemical change in the polymer matrix, the change being indicative of the presence of the microorganism. | 03-03-2016 |
20160061834 | NOROVIRUS DETECTION SENSOR AND ELECTROCHEMICAL SENSING METHOD USING THE SAME - Disclosed herein are a norovirus detection sensor and an electrochemical sensing method using the sensor. Specifically, in the norovirus detection sensor including a bioreceptor and a signal detector, a three-dimensional gold nanosurface electrode is used as a substrate, and the bioreceptor employs concanavalin A as a sample capture agent immobilized to the substrate and capable of binding to norovirus. Therefore, the norovirus detection sensor has improved sensitivity by employing the three-dimensional gold nanosurface electrode having a wide surface area. In addition, the norovirus detection sensor has effects of reducing manufacturing costs using a non-antibody material, i.e., concanavalin A which is inexpensive and readily available. | 03-03-2016 |
20160068918 | METHODS FOR ASSESSING FATIGUE LEVEL AND APPLICATIONS THEREOF - Level of fatigue that accompanies everyday life or a disease can be simply, easily, and quantitatively assessed by obtaining a body fluid from a test subject and measuring the amount of human herpesvirus in the body fluid. Furthermore, the anti-fatigue potency of anti-fatigue substances and anti-fatigue food products can be measured. | 03-10-2016 |
20160069873 | FLOW CHANNEL DEVICE AND DETECTION METHOD USING SAME - A flow channel device includes a flow channel section and an introduction channel section. The flow channel section includes a flow channel in which a detection object flows and a wall surface surrounding the flow channel. The introduction channel section includes an introduction channel having a first end connected to the flow channel and a second end connected to an introduction port, and a wall surface surrounding the introduction channel. At least a part of the wall surface surrounding the introduction channel is a curved surface protruding toward the introduction channel. | 03-10-2016 |
20160069918 | BIOLOGICAL SAMPLE PROCESSING - Systems and methods are provided for processing a biological sample. In one embodiment, the method comprises receiving a sample vessel containing the sample; retrieving information from an information storage unit associated with the sample; using said information for selecting at least one cartridge from at least two or more different cartridges, each configured for use with a sample processing device; loading at least one or more reagents onto the cartridge, wherein the one or more reagents to be added are selected based at least in part on the information or instructions derived from the information; and placing the sample vessel in the cartridge. | 03-10-2016 |
20160076041 | Foreign DNA Surveillance Protein - A method for augmenting expression of a heterologous nucleic acid in a eukaryotic cell or increasing the efficiency of gene expression using any gene expression system is carried out by decreasing expression or activity of an endogenous Interferon-induced protein-16 (IFI16). | 03-17-2016 |
20160076981 | PROCESSING BIOLOGICAL MATERIAL FOR FLOW CYTOMETRY EVALUATION FOR VIRUS PARTICLES - In a method for processing biological materials for flow cytometry evaluation for virus particles, a mixture including biological material and purification particles is centrifuged to prepare a centrifuged composition including a supernatant that may be further processed prior to the flow cytometry evaluation. The purification particles include porous cores functionalized to capture smaller-size impurities in a biological material sample and a porous size-exclusion shell surrounding the core to exclude larger-size components of the biological material from entering into the core. Multiple samples may be processed in multi-sample processing units. A product may contain a sealed container with the unit quantity of purification particle in a storage liquid and a kit may include such a sealed container and a centrifugal filter. | 03-17-2016 |
20160077090 | NANOCOMPOSITES AND METHODS OF MAKING SAME - A nanoagent includes at least one nanocomposite. The nanocomposite includes at least one gold nanorod, a silver layer coated on an outer surface of the gold nanorod, a Raman reporter molecule layer coated on the silver layer, a pegylated layer coated on the Raman reporter molecule layer, an active layer conjugated to the pegylated layer. the active layer includes at least one of a targeting molecule configured to bind to a target of interest, and a functional molecule configured to interact with the target of interest. The silver layer has silver nanoparticles. The Raman reporter molecule layer has Raman reporter molecules that are detectable by surface enhanced Raman spectroscopy (SERS). The pegylated layer has at least one of thiolated polyethylene glycol (HS-PEG), thiolated polyethylene glycol acid (HS-PEG-COOH) and HS-PEG-NHx. | 03-17-2016 |
20160083716 | Highly Simplified Lateral Flow-Based Nucleic Acid Sample Preparation and Passive Fluid Flow Control - Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays. | 03-24-2016 |
20160083776 | Methods for Measuring Polymerase Activity Useful for Sensitive, Quantitative Measurements of Any Polymerase Extension Activity and for Determining the Presence of Viable Cells - A novel, highly sensitive, quantitative and rapid DPE-PCR assay is disclosed that can be used to enumerate prokaryotic cells when presenting a purified or selected cell type and that has the capability to reproducibly measure DNA polymerase extension activity from less than 10 cfu of bacteria via coupling to bead lysis. Also disclosed is the potential for the DPE-PCR assay of the invention to universally detect microbes by testing a panel of microorganisms comprised of gram-negative bacteria, gram-positive bacteria and | 03-24-2016 |
20160083783 | NOVEL HYBRIDIZATION PROBES AND USES THEREOF - The present invention relates to novel hybridization probes useful for rapid hybridization to realize a practicable and affordable pathogen diagnostic based on RNA signature detection, technology and hardware. In particular, the present invention relates to a nucleic acid structure which may comprise nucleic acid tiles, wherein each nucleic acid tile comprises nucleic acid oligomers, wherein each nucleic acid oligomer hybridizes to each other thereby forming a nucleic acid tile. | 03-24-2016 |
20160084835 | METHODS FOR DIAGNOSING INFECTIOUS DISEASES USING ADSORPTION MEDIA - The present invention provides an in vitro method for concentrating infectious pathogens found in a biological sample obtained from an individual who is suspected of being infected with the pathogens. Provided herein is also an in vitro method for reducing or eliminating blood cells from a sample obtained from an individual suspected to being infected with an infectious pathogen. The present invention also provides a method for diagnosing malaria and a method for determining if an individual is infected with a pathogen. Provided herein is also a concentrator and a kit for use with the methods. | 03-24-2016 |
20160084842 | WOVEN HYDROGEL BASED BIOSENSOR - A porous hydrogel sensor that is responsive to the presence of one or more target compounds in solution is synthesized based on demixing of certain molecules in the presence of a target compound. The porous hydrogel sensor may include fluorescently tagged antibodies that are noncovalently bound to the gel and then released in the presence of the target antigen. The porous hydrogel sensor may alternatively include dissolvable cross-links using polymerized antibody and antigen complexes so that, in the presence of the target antigen, the cross-links will be displaced and the hydrogel will dissolve. | 03-24-2016 |
20160086781 | RARE EVENT DETECTION USING MASS TAGS - The invention generally relates to methods for rare event detection using mass tags. In certain embodiments, the invention provides methods for detecting a target analyte in a sample that involve conducting an assay that specifically associates a mass tag with a target analyte in a sample, generating ions of the mass tag, and analyzing the generated ions of the mass tag, thereby detecting the target analyte from the sample. | 03-24-2016 |
20160090588 | DEVICE FOR PREPARATION AND ANALYSIS OF NUCLEIC ACIDS - An integrated “lab-on-a-chip” microfluidic device performs nucleic acid sample preparation and diagnostic analysis from test samples containing cells and/or particles. The device analyzes DNA or RNA targets, or both, from a common test sample. Dried and/or liquid reagents necessary for nucleic acid sample preparation and analysis are contained on the device, such that the device only requires addition of test sample. Clay mineral and alkaline buffer reagents are employed for overcoming the problems of nucleic acid degradation and contamination during sample preparation. The device may include a composite filter to separate plasma or serum from other blood constituents when the test sample is a blood product. The microfluidic device utilizes a plurality of microfluidic channels, inlets, valves, membranes, pumps, and other elements arranged in various configurations to manipulate the flow of the liquid sample, in particular, in order to prepare nucleic acids and perform further diagnostic analysis. | 03-31-2016 |
20160090640 | METHOD FOR THE CONCENTRATION AND DETECTION OF VIRUS - Disclosed is an economic method for concentrating virus and detecting virus, such that virus in a sample solution having low virus concentration can be concentrated with high efficiency within a short time. Particularly, the method comprising the steps of: (A) adding Concanavalin A (Con A) to a sample solution containing a virus, and reacting the added Concanavalin A with the virus in the sample solution to form a virus-Concanavalin A conjugate; and (B) separating the virus-Concanavalin A conjugate from the sample solution. | 03-31-2016 |
20160091506 | TWO PART ASSEMBLY - A device that includes a first portion, the first portion including at least one fluid channel; a fluid actuator; and an introducer; a second portion, the second portion including at least one well, the well containing at least one material, wherein one of the first or second portion is moveable with respect to the other, wherein the introducer is configured to obtain at least a portion of the material from the at least one well and deliver it to the fluid channel, and wherein the fluid actuator is configured to move at least a portion of the material in the fluid channel. | 03-31-2016 |
20160096802 | PROCESS FOR PREPARING GUANIDINO-FUNCTIONAL MONOMERS - A process for preparing guanidino-functional, free radically polymerizable compounds comprises (a) combining (1) an amine compound comprising (i) at least one primary aliphatic amino group and (ii) at least one secondary aliphatic amino group, primary aromatic amino group, or secondary aromatic amino group, and (2) a guanylating agent; (b) allowing or inducing reaction of the amine compound and the guanylating agent to form a guanylated amine compound; (c) combining (1) the guanylated amine compound, and (2) a reactive monomer comprising (i) at least one ethylenically unsaturated group and (ii) at least one group that is reactive with an amino group; and (d) allowing or inducing reaction of the guanylated amine compound and the reactive monomer to form a guanidino-functional, free radically polymerizable compound. | 04-07-2016 |
20160102296 | INFECTIOUS HEPATITIS E VIRUS GENOTYPE 3 RECOMBINANTS - The invention relates to the discovery of an HEV strain from a chronically infected patient. The virus grow unusually well in numerous cell cultures. Thus, the invention provides cell cultures, vectors, and vaccine compositions based on the virus. | 04-14-2016 |
20160102340 | METHODS FOR PREPARATION OF NUCLEIC ACID-CONTAINING SAMPLES USING CLAY MINERALS AND ALKALINE SOLUTIONS - The present invention provides a rapid and highly effective method for the preparation of biological samples for detection of both DNA and RNA target molecules. The method comprises treatment of the sample with a clay mineral followed by lysis of the sample with an alkaline solution. The method is particularly suited for preparing complex biological samples, such as blood or plasma, for the simultaneous detection of DNA and RNA targets. Samples prepared by the method of the invention may be directly used as targets in PCR amplification. The method of the invention may conveniently be coupled with further steps and devices to perform molecular analyses for diagnostic and other applications. | 04-14-2016 |
20160103135 | METHOD - The invention provides a method of detecting the presence of anti-MHC antibodies in a sample comprising contacting said sample with one or more recombinant MHC molecules or functionally equivalent variants, derivatives or fragments thereof and detecting the binding or absence of binding of antibodies to said recombinant MHC molecules. This method allows the detection and/or identification of one or more specific MHC particularly HLA allele antibodies. | 04-14-2016 |
20160106076 | RECOMBINANT NON-HUMAN MAMMALIAN MODEL FOR HEPATITIS INFECTION AND IMMUNOPATHOGENESIS - Provided herein is a recombinant non-human mammal having an immune system including human immune cells and having a liver including human liver cells, and methods for producing the same. Also provided are methods of screening a compound for activity in treating hepatitis, comprising: administering a test compound to a recombinant non-human mammal as described herein; and then detecting the presence or absence of said activity in said mammal (e.g., by biochemical assay), said presence of said activity in said mammal indicating that said compound has activity in treating hepatitis. Methods of making fusion cells useful for the production of human monoclonal antibodies are also provided. | 04-21-2016 |
20160109441 | APPARATUS FOR DETECTING BIO MATERIALS, METHOD OF FABRICATING THE APPARATUS AND METHOD OF DETECTING BIO MATERIALS USING THE APPARATUS - Provided are an apparatus for detecting bio materials, a method of fabricating the same and a method of detecting bio materials using the same. The apparatus for detecting bio materials includes a filter, freeze dried bio material detecting materials and bio material capturing materials fixed to the inner wall of a microtube, arranged in order in the microtube. Antibodies capturing blood cells in blood are combined with the filter, each of the bio material detecting materials includes immunogolds conjugated with detecting antibodies. The apparatus for detecting bio materials is portable and perceive a small amount of the bio materials with high sensitivity. | 04-21-2016 |
20160109451 | COMPOSITIONS AND METHODS FOR SIMULTANEOUS DETECTION OF HCV ANTIGEN/ANTIBODY - Compositions and processes are provided for the robust detection of hepatitis C virus in a sample. Compositions include amino acid sequences of HCV core region including or exclusive to residues 14-31 or 50-90 of the HCV core protein. Also provided are processes of detecting HCV in a sample whereby the provided peptides are optionally used alone or in conjunction with antibodies that do not recognize the peptides so that detection is independent of seroconversion in a subject. Using the compositions and processes, HCV can be detected in a sample prior to or following seroconversion leading to robust HCV detection and diagnosis. | 04-21-2016 |
20160116458 | CELL MODEL FOR NEOVASCULAR DISEASES USING EBV-INFECTED HUMAN CORNEAL EPITHELIAL CELLS - The present invention relates to a cell model for diseases associated with corneal neovascularization by using Epstein Barr virus (EBV)-infected human corneal epithelial cells (HCECs). Provided are a method for preparing a cell model for diseases associated with corneal neovascularization by using EBV-infected HCECs, the method including: infecting HCECs with EBV; culturing the infected HCECs; and determining whether the cultured HCECs are infected with EBV. In addition, provided is a method for screening diseases associated with corneal neovascularization prepared by the cell model for diseases associated with corneal neovascularization. | 04-28-2016 |
20160116464 | STIMULI-RESPONSIVE MAGNETIC NANOPARTICLES - The present disclosure provides stimuli-responsive magnetic nanoparticles, methods of making the magnetic nano-particles, and methods of using the magnetic nanoparticles. The magnetic nanoparticles include a metal oxide core; and a shell that includes a stimuli-responsive polymer having a terminal group that directly coordinates to the metal oxide core. The stimuli-responsive polymer does not include a micelle-forming group, at least at a proximal terminus of the polymer, with respect to the metal oxide core. | 04-28-2016 |
20160116471 | METHOD FOR THE DETECTION AND CLASSIFICATION OF PRRSV-INFECTIONS IN SWINE HERDS AND DIAGNOSTIC ANTIGEN COMPOSITIONS FOR SUCH METHODS - Method for the detection and classification of PRRSV-infections in swine herds, comprising a) the incubation of tissue samples taken from the animals with at least one antigen capable to bind a neutralizing antibody against the Type I-virus possibly present in the animal and with at least one antigen capable to bind a neutralizing antibody against the Type II-virus possibly present in the animal, b) testing whether a binding of antibodies against the Type I-virus and/or the Type II-virus has taken place and c) determining from the presence of possible epitope-antibody complexes whether an infection of the PRRSV I-Type and/or PRRSV II-Type is present in the herd and diagnostic compositions for such a method. | 04-28-2016 |
20160116481 | PREPARATION OF VISIBLE COLORED INSOLUBLE CARRIER PARTICLES LABELED WITH A FLUORESCENT DYE AND AN IMMUNOASSAY METHOD USING THE SAME - The object of the present invention is to provide insoluble carrier particles used for high-sensitivity rapid immunoassay, which allow visual observation and high-sensitivity apparatus measurement. | 04-28-2016 |
20160122811 | COMPOSITIONS AND METHODS FOR AMPLIFYING A NUCLEIC ACID SEQUENCE IN A SAMPLE - The present invention features compositions and methods for amplifying a target oligonucleotide in a sample comprising one or more primer oligonucleotides comprising a 5′ nicking enzyme recognition site and a 3′-terminal region comprising a 2′-modified nucleotide. These methods are compatible with target oligonucleotides amplified using a nicking amplification reaction. | 05-05-2016 |
20160122834 | METHODS OF DETECTING EBOLA - Compositions and methods for detecting Ebola are provided. | 05-05-2016 |
20160123975 | Mesh Microfluidic Mixing Chamber - This document provides devices, systems, and methods for detecting pathogens in a biological sample. In some cases, the devices, systems, and methods include a microfluidic device, which includes a biological sample inlet in fluid communication with at least a first microfluidic chamber. The first microfluidic chamber holds a fibrous matrix. The fibrous matrix can carrying anticoagulant and target capture agents within the fibrous matrix. In some cases, the fibrous matrix can be a nonwoven sheet, which can entangle and retain particles bonded to the target capture agents. When in use, a biological sample is introduced into a microfluidic device to flow through the fibrous matrix to mix with the anticoagulant and contact target capture agents. The target capture agents can either remain in the fibrous matrix or flow to a target capture agent chamber. A lysis agent can be delivered through microfluidic chambers including the target capture agent and any captured targets to lyse the target and produce lysate, which can be analyzed to detect a presence of the target in the biological sample. | 05-05-2016 |
20160123981 | A METHOD OF DETERMINING VIRUS REMOVAL FROM A SAMPLE CONTAINING A TARGET PROTEIN USING ACTIVATED CARBON - The present invention provides methods for determining whether activated carbon can be used for removing viruses or a certain virus from a sample containing a target protein. | 05-05-2016 |
20160123990 | AAV VECTOR AND ASSAY FOR ANTI-AAV (ADENO-ASSOCIATED VIRUS) NEUTRALIZING ANTIBODIES - Virus vectors, virus particles, and methods and uses of screening for, detecting, analyzing and determining amounts of virus antibody, or neutralizing antibody activity of samples are provided. Such virus vectors, virus particles, and methods and uses are applicable to a broad range of virus types, such as lentiviruses, adenovirus, and adeno-associated virus (AAV) serotypes. Methods and uses include virus antibody screening, such as anti-virus immunoglobulins screened for, detected, analyzed and amounts determined | 05-05-2016 |
20160130673 | NUCLEIC ACID DETECTION BY OLIGONUCLEOTIDE PROBES CLEAVED BY BOTH EXONUCLEASE AND ENDONUCLEASE - Disclosed is a method in the fields of biochemistry and molecular biology. The method is related to improve cleavage kinetics of labeled oligonucleotide probes and, consequently, increases signal-to-noise ratio in detecting nucleic acids. | 05-12-2016 |
20160131591 | COLIPHAGE BIOSENSOR - Disclosed are methods, apparatuses, and genetically modified bacteria that may be used to detect bacteriophages in a sample. In some embodiments, a rapid detection test is disclosed to test for the presence of coliphages which may indicate the presence of human or animal waste contamination in water samples. | 05-12-2016 |
20160131650 | VIRUS-DETECTION KITS AND METHODS OF MAKING AND USING THE SAME - The present disclosure relates to compositions, kits, and methods for the detection of a virus in a sample. Typically, the sample is from a subject suspected of, exhibiting symptoms of, or previously diagnosed with a viral infection, such as an Ebola virus. | 05-12-2016 |
20160131653 | SUBJECT ANIT-HCV ANTIBODY DETECTION ASSAYS EMPLOYING NS3 CAPTURE PEPTIDES - The present disclosure provides methods, kits, and compositions for detecting subject anti-HCV antibodies in a sample using NS3 capture peptides. In certain embodiments, at least two NS3 helicase (NS3h) capture peptides and at least two conjugate peptides (e.g., NS3h conjugate peptides) are employed together, which allows for a broad dynamic range of subject antibody detection in a one-step type assay. In other embodiments, methods are provided of detecting NS3-specific subject antibodies without the use of a reducing agent. In some embodiments, NS3-specific subject antibodies are detected with a ‘double shot’ of NS3 conjugate peptide (e.g., conjugate peptide added to a sample both before and after washing). | 05-12-2016 |
20160139052 | MICROFLUIDIC BIOSENSING SYSTEM - Disclosed is a microfluidic biosensing system including a processor, in which a Raman barcode database corresponding to at least one Raman spectrum signal is stored, a plurality of Raman barcode beads mixed with a target fluid and coupled to at least one target bioparticle in the target fluid, a microfluidic channel disposed to make the target fluid mixed with the Raman barcode beads flow therethrough, a light source disposed on the microfluidic channel, and a spectral detection device connected to the processor and disposed to correspond to the light source. The spectral detection device receives the Raman spectrum signal generated when the target bioparticle coupled with the Raman barcode bead is irradiated, and transfers the received Raman spectrum signal to the processor. The processor determines a type of the bioparticle(s) and calculates the number of bioparticle(s) by matching the Raman spectrum signal(s) to the Raman barcode database. | 05-19-2016 |
20160139125 | IMMUNOCHROMATOGRAPHY DEVICE FOR DETECTING RSV - The present invention provides an easy-to-use and higher-sensitivity immunochromatography device for an RSV, the device using an antibody that binds specifically to F protein of the RSV in a determination region of a chromatography medium, a test kit by an immunochromatography method, and a method for detecting an RSV using these device and kit. The present invention relates to an immunochromatography device in which not only an antibody that binds specifically to F protein of an RSV but also an antibody that binds specifically to N protein of the RSV is solid-phased in a determination region of a chromatography medium. The present invention also relates to a test kit by an immunochromatography method, and a method for detecting an RSV using these device and kit. | 05-19-2016 |
20160145698 | PRIMERS AND PROBES FOR DETECTION AND DISCRIMINATION OF EBOLA VIRUS - This invention relates to primers and probes for detecting Ebola virus and one or more subtypes of Ebola virus as well as kits including the probes and primers and methods of using the probes and primers. | 05-26-2016 |
20160146714 | MONOLITHS - Described herein are monoliths for processing fluid samples, and methods of making and using such monoliths. | 05-26-2016 |
20160146755 | DEVICES, SYSTEMS, AND METHODS FOR ELECTROPHORESIS - Disclosed herein are devices that can be used to perform electrophoretic separations as well as methods of using thereof. The devices and methods described herein are inexpensive, user friendly, sensitive, portable, robust, efficient, thin, rapid, and use low voltage. As such, the device and methods are well suited for use in numerous applications including point-of-care (POC) diagnostics. | 05-26-2016 |
20160146797 | SYSTEMS AND METHODS FOR THE CAPTURE AND SEPARATION OF MICROPARTICLES - Systems and methods are provided for capturing and/or isolating target microparticles. In one aspect, a method for capturing target microparticles is disclosed. The method includes: forming a fluid including the target microparticles, non-target microparticles, and magnetic beads, the magnetic beads having a stronger affinity with the target microparticles than with the non-target microparticles; flowing the fluid through a multidirectional microchannel; and applying a magnetic field to the fluid while the fluid is flowing through at least a portion of the microchannel to effect capture of at least a portion of the target microparticles onto the magnetic beads. Such a method can further includes passing the fluid having exited from the microchannel through a separator while subjecting the fluid to a second magnetic field so as to isolate the target microparticles. In addition, devices and systems are disclosed for capturing and/or isolating target microparticles based on magnetic manipulation. | 05-26-2016 |
20160146813 | MULTIFUNCTIONAL NANOPROBE-ENABLED CAPTURE AND EARLY DETECTION OF MICROBIAL PATHOGENS - The presently disclosed fluidic sensor system and method comprise multifunctional nanoprobe-enabled capture for early detection of chemical and/or biological pathogens in a liquid sample. This sensor system and method can be used for food and environmental monitoring. | 05-26-2016 |
20160151780 | DEVICE AND METHOD FOR STORING AND TRANSPORTING A BODILY FLUID SAMPLE | 06-02-2016 |
20160153059 | A METHOD OF CERVICAL SCREENING | 06-02-2016 |
20160153060 | METHOD FOR SPECIES IDENTIFICATION BY USING MOLECULAR WEIGHTS OF NUCLEIC ACID CLEAVAGE FRAGMENTS | 06-02-2016 |
20160153972 | NOVEL FLOW ASSAY METHOD FOR AN OBJECT OF INTEREST | 06-02-2016 |
20160153975 | NANOCOMPOSITES, METHODS OF MAKING SAME, AND APPLICATIONS OF SAME FOR MULTICOLOR SURFACE ENHANCED RAMAN SPECTROSCOPY (SERS) DETECTIONS | 06-02-2016 |
20160161519 | SYSTEMS AND METHODS FOR DETECTING MATERIALS IN FOOD PRODUCTS - A device, method and system for measuring analytes in a solution or suspension. The device includes a housing, a sample chamber, one or more pumps, one or more detectors, one or more stirrers, electrical contacts, a magnetic manipulator and a lid. A sample cartridge for a device that measures analytes, the cartridge comprising a first outer compartment, a second outer compartment, and a middle compartment, wherein the first outer compartment and the second outer compartment are interconnected to the middle compartment by channels. | 06-09-2016 |
20160168615 | COMPOSITIONS AND METHODS FOR DETECTING PATHOGENS | 06-16-2016 |
20160169788 | EMULSION | 06-16-2016 |
20160169873 | LABEL-FREE DETECTION OF SMALL AND LARGE MOLECULE INTERACTIONS, AND ACTIVITIES IN BIOLOGICAL SYSTEMS | 06-16-2016 |
20160169924 | DETAILED ASSAY PROTOCOL SPECIFICATION | 06-16-2016 |
20160177366 | HAND-HELD MICRO-RAMAN BASED DETECTION INSTRUMENT AND METHOD OF DETECTION | 06-23-2016 |
20160177367 | MICROSENSOR | 06-23-2016 |
20160178623 | Assay Device Having Multiple Reagent Cells | 06-23-2016 |
20160178653 | CARTRIDGE FOR STORING BIOSAMPLE CAPILLARY TUBES AND USE IN AUTOMATED DATA STORAGE SYSTEMS | 06-23-2016 |
20160185814 | VIRUS DETECTION - The invention provides methods and materials for use in the detection of influenza viruses which utilise a nanoparticle, for example gold nanoparticle, probe comprising a plurality of glycoconjugate ligands, each glyconjugate ligand (GL) having a plurality of sialic-acid containing recognition group (Y) coupled to the nanoparticle via a multivalent core (X), wherein the multivalent core (X) is a trivalent core, whereby there are 3 recognition groups per ligand, wherein the recognition groups on the bioconjugate specifically bind to the hemagglutinin on the target influenza virus. The probes may include further ligands bound to the nanoparticle which do not bind specifically to an influenza virus—for example polyethylene glycol groups. These can modulate density of the glycoconjugate ligand on the surface of the nanoparticle. Binding of probes is detected by a plasmonic signal which is specific to the influenza virus. | 06-30-2016 |
20160186272 | COMPOSITIONS AND METHODS FOR DETECTING HEV NUCLEIC ACID - Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of Hepatitis E Virus (HEV) nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits. | 06-30-2016 |
20160187329 | METHOD OF AMPLIFYING DETECTION LIGHT USING LIGHT-REFLECTING MATERIAL IN IMMUNOCHROMATOGRAPHY - The present invention intends to provide an immunochromatographic test piece that makes it possible to achieve both highly sensitive detection of a substance to be detected and a simple test piece structure, which are usually difficult to be made compatible with each other. The immunochromatographic test piece is an immunochromatographic test piece comprising a membrane on which a capture substance being a ligand that bonds to a substance to be detected is immobilized, wherein insoluble carrier particles to which a ligand that bonds to the substance to be detected is bound are used and accumulated by being captured with the capture substance immobilized on the membrane, the membrane is irradiated with light to detect light emitted from a portion where the insoluble carrier particles are accumulated or light emitted from a portion surrounding and other than the portion where the insoluble carrier particles are accumulated, thereby measuring the substance to be detected, and a light-reflecting material is provided on a side of the membrane opposite to a side irradiated with light. | 06-30-2016 |
20160187337 | ASSAY FOR JC VIRUS ANTIBODIES - The disclosure relates to methods and reagents for analyzing samples for the presence of JC virus antibodies. Disclosed is a method that includes obtaining a biological sample from a subject (e.g., plasma, serum, blood, urine, or cerebrospinal fluid), contacting the sample with highly purified viral-like particles HPVLPs) under conditions suitable for binding of a JCV antibody in the sample to an HPVLP, and detecting the level of JCV antibody binding in the sample to HPVLP In one embodiment, determining the level of anti-JCV antibodies in the subject sample provides a method of identifying PML risk in a subject. | 06-30-2016 |
20160188790 | ROTAVIRUS PARTICLES WITH CHIMERIC SURFACE PROTEINS - The present invention relates to the use of rotavirus particles for displaying a heterologous protein, alone or in complex with another molecule. The invention further relates to methods that employ these modified rotavirus particles to rapidly determine the structure of the heterologous protein or the complex using cryo-electron microscopy (cryo-EM). The invention also relates to a method of immunising a patient, wherein said method comprises administering to the patient the modified rotavirus particles of the invention. | 06-30-2016 |
20160193602 | ORIENTED LOADING SYSTEMS AND METHOD FOR ORIENTING A PARTICLE LOADED IN A WELL | 07-07-2016 |
20160194612 | HIGH-TITER HCV FULL-LENGTH GENOTYPE 2B INFECTIOUS CELL CULTURE SYSTEMS AND APPLICATIONS THEREOF | 07-07-2016 |
20160194687 | METHODS AND COMPOSITIONS FOR DIRECT CHEMICAL LYSIS | 07-07-2016 |
20160201149 | METHOD FOR PREDICTION OF PROGNOSIS BY HUMAN PAPILLOMAVIRUS DNA INTEGRATION PATTERN | 07-14-2016 |
20160202222 | Method, Device, And System For Aerosol Detection Of Chemical And Biological Threats | 07-14-2016 |
20160202251 | VIRUS-LIKE PARTICLE FOR USE IN IMMUNOASSAY, BLOCKING AGENTFOR USE IN THE IMMUNOASSAY, AND KIT COMPRISING THEVIRUS-LIKE PARTICLE AND THE BLOCKING AGENT | 07-14-2016 |
20160202259 | APTAMERS SCREENING METHOD BASED ON GRAPHENE WITHOUT TARGET IMMOBILIZATION AND THE APTAMERS OBTAINED FROM THE METHOD | 07-14-2016 |
20160202265 | METHOD OF DETERMINING RESISTANCE TO INFLUENZA VIRUS | 07-14-2016 |
20160251724 | METHODS AND DEVICES FOR NASOPHARYNGEAL CARCINOMA SCREENING | 09-01-2016 |
20160252502 | DETECTION ASSAYS AND METHODS | 09-01-2016 |
20160252504 | TARGET TESTING DEVICE, TARGET TESTING KIT, TARGET TESTING METHOD, TRANSFER MEDIUM, AND METHOD FOR PRODUCING TARGET TESTING DEVICE | 09-01-2016 |
20160377592 | SINGLE-USE HANDHELD DIAGNOSTIC TEST DEVICE, AND AN ASSOCIATED SYSTEM AND METHOD FOR TESTING BIOLOGICAL AND ENVIRONMENTAL TEST SAMPLES - In a single-use handheld diagnostic test device and an associated system and method, a biological and/or environmental test sample is received and reacted with reagents. The test device tests a single sample and includes a sensor to detect test data. The test device mates with, and transmits the test data to, an electronic device. A processor of the electronic device applies algorithms to the test data to generate highly sensitive and accurate quantitative test results. A presentation element of the electronic device presents the test results to a user. The test device is adapted for disposal, or for sterilization and re-use, after the electronic device receives the test data. The electronic device may be, for example, a mobile communications device, a personal digital assistant, a laptop computer, a navigation device, a digital audio player, a camera, or a gaming device. | 12-29-2016 |
20160377599 | LIVER INFECTION - The invention relates to a method for studying an infection process in liver tissue in vitro, the method comprising: seeding hepatocyte cells onto a scaffold in a bioreactor in order to form a liver tissue model;delivering an infectious agent to the liver tissue model, or providing the liver tissue model pre-infected with an infectious agent; and monitoring the infection process; and a method for producing an infectious agent. | 12-29-2016 |
20160377616 | IMMUNOCHROMATOGRAPHIC DETECTION METHOD - The present invention provides a detection method using an immunochromatographic test strip that suppresses the occurrence of a so-called white bleaching phenomenon and that is accurate and excellent in sensitivity. The present invention provides an immunochromatographic detection method that uses an immunochromatographic test strip including (1) and (2) below and that comprises the steps of (A) and (B) below: (1) a conjugate pad having a sample-supply portion for supplying a sample possibly containing an analyte, and a conjugate portion on the downstream side of the sample-supply portion, the conjugate portion containing a conjugate in which an antibody to the analyte is immobilized on a label, (2) an insoluble membrane support having at least one detecting section on which an antibody to the analyte is immobilized, (A) a step of supplying methanol and the sample possibly containing the analyte to the sample-supply portion, and (B) a step of detecting the analyte as an immune reaction product on the insoluble membrane support. | 12-29-2016 |
20170232097 | Live Attenuated Viral Vaccine Created by Self-Attenuation With Species-Specific Artificial MicroRNA | 08-17-2017 |
20170233794 | Nucleic Acid Detection System and Method for Detecting Influenza | 08-17-2017 |
20170233798 | NMR SYSTEMS AND METHODS FOR THE RAPID DETECTION OF ANALYTES | 08-17-2017 |
20170233832 | Quantitative measurement of Hepatitis B virus cccDNA | 08-17-2017 |
20170234876 | RAPID TEST TO DETERMINE THE NECESSITY OF REVACCINATION | 08-17-2017 |
20170234877 | HUMAN FACTOR XIII AS A NORMALIZATION CONTROL FOR IMMUNOASSAYS | 08-17-2017 |
20180023058 | EMPLOYING HUMAN ADIPOSE-DERIVED STEM CELLS TO PROPAGATE SERUM-DERIVED HEPATITIS C VIRUS AND USE THEREOF | 01-25-2018 |
20180024126 | NANOCOMPOSITES, METHODS OF MAKING SAME, AND APPLICATIONS OF SAME FOR MULTICOLOR SURFACE ENHANCED RAMAN SPECTROSCOPY (SERS) DETECTIONS | 01-25-2018 |
20190143328 | HIGH-THROUGHPUT PARTICLE CAPTURE AND ANALYSIS | 05-16-2019 |
20190144925 | Compositions and Methods for Identifying, Quantifying, and/or Characterizing an Analyte | 05-16-2019 |
20190145952 | METHOD AND SYSTEM FOR DETECTION OF DISEASE AGENTS IN BLOOD | 05-16-2019 |
20190148014 | BIOFLUIDIC TRIGGERING SYSTEM AND METHOD | 05-16-2019 |
20220136969 | RAPID PATHOLOGY/CYTOLOGY WITHOUT WASH - Among other things, the disclosure of the present invention is related to make pathology and cytology faster, better and lower cost. The present invention also related to rapid intracellular assay. | 05-05-2022 |
20220137049 | Systems and Methods for Assay Processing - Methods for obtaining a sample from a subject include providing a sample collection room within a retail store; and obtaining a sample from a subject at a retail location. Sample collection rooms may house a sample analysis device or system. Samples may be small, e.g., a finger-stick capillary blood sample. | 05-05-2022 |
20220137050 | METHOD FOR DIAGNOSING HUMAN T-CELL LEUKEMIA VIRUS TYPE 1 (HTLV-1) ASSOCIATED DISEASES - As a technique enabling to simply and accurately diagnose human T cell leukemia virus type 1 (HTLV-1) related disease, there is provided a diagnostic method for an HTLV-1 related disease, comprising the steps of: measuring the amount of a marker protein in a blood sample taken from a subject, wherein the marker protein is at least one selected from the proteins listed in Tables 1 and 2; and, if a measured value is a predetermined reference value or more or the predetermined reference value or less, determining that the subject suffers from the HTLV-1 related disease or determining that the subject is in remission of the HTLV-1 related disease. | 05-05-2022 |