Entries |
Document | Title | Date |
20080213872 | Disposable and Removable Nucleic Acid Extraction and Purification Cartridges For Automated Flow-Through Systems - Removable cartridges are used on automated flow-through systems for the purpose of extracting and purifying genetic material from complex matrices. Different types of cartridges are paired with specific automated protocols to concentrate, extract, and purifying pathogenic or human genetic material. Their flow-through nature allows large quantities sample to be processed. Matrices may be filtered using size exclusion and/or affinity filters to concentrate the pathogen of interest. Lysed material is ultimately passed through a filter to remove the insoluble material before the soluble genetic material is delivered past a silica-like membrane that binds the genetic material, where it is washed, dried, and eluted. Cartridges are inserted into the housing areas of flow-through automated instruments, which are equipped with sensors to ensure proper placement and usage of the cartridges. Properly inserted cartridges create fluid- and air-tight seals with the flow lines of an automated instrument. | 09-04-2008 |
20080220506 | Tissue scaffold having aligned fibrils, apparatus and method for producing the same, and artificial tissue and methods of use thereof - A tubular tissue scaffold is described which comprises a tube having a wall, wherein the wall includes biopolymer fibrils that are aligned in a helical pattern around the longitudinal axis of the tube where the pitch of the helical pattern changes with the radial position in the tube wall. The scaffold is capable of directing the morphological pattern of attached and growing cells to form a helical pattern around the tube walls. Additionally, an apparatus for producing such a tubular tissue scaffold is disclosed, the apparatus comprising a biopolymer gel dispersion feed pump that is operably connected to a tube-forming device having an exit port, where the tube-forming device is capable of producing a tube from the gel dispersion while providing an angular shear force across the wall of the tube, and a liquid bath located to receive the tubular tissue scaffold from the tube-forming device. A method for producing the tubular tissue scaffolds is also disclosed. Also, artificial tissue comprising living cells attached to a tubular tissue scaffold as described herein is disclosed. Methods for using the artificial tissue are also disclosed. | 09-11-2008 |
20080220507 | Kit for Packaging Predetermined Volume of Substance to be Preserved by Cryogenic Vitrification - The invention concerns an assembly for packaging a volume of substance to be preserved by cryogenic vitrification characterized in that it comprises a sheathing ( | 09-11-2008 |
20080248560 | Method of Treatment of Tissue Processing Fluid and Apparatus Therefor - The present invention relates to the field of tissue processing, particularly, to the treatment of fluids used in tissue processing. In one embodiment the present invention provides for treating a tissue processing fluid comprising first processing fluid comprising a first processing fluid and a second contaminating fluid, by: gradually varying the pressure of the fluid from an initial pressure level to a first pressure level such that the fluid remains below a vaporizing point of the second contaminating fluid and, heating the fluid to at least a vaporizing temperature of the second contaminating fluid. In another embodiment, the present invention removes the second contaminating fluid from the fluid by varying at least one of the pressure and temperature of the fluid so as to allow the second contaminating fluid to vaporize. Alternatively, an embodiment of the present invention interleaves the step of removing with one or more tissue processing protocol steps. In a preferred embodiment, the invention relates to the treatment of infiltrating materials, particularly in their liquid form in a histological tissue sample processor capable of Xylene free operation. | 10-09-2008 |
20080286856 | Nanostructure Enhanced Luminescent Devices - The present invention relates to nanostructures for use in luminescent devices. | 11-20-2008 |
20080293127 | Method to Render Pressure Sensitive Adhesive Compatible with Polymerase Chain Reaction Systems - A method for substantially eliminating a cross-linking element of a pressure sensitive adhesive by heating the pressure sensitive adhesive at a temperature of at least 180° C. for a period of time required to thermally decompose the cross-linking element of the pressure sensitive adhesive. | 11-27-2008 |
20080318305 | IN SITU HEAT INDUCED ANTIGEN RECOVERY AND STAINING APPARATUS AND METHOD - An automated in situ heat induced antigen recovery and staining method and apparatus for treating a plurality of microscope slides. The process of heat induced antigen recovery and the process of staining the biological sample on the microscope slide are conducted in the same apparatus, wherein the microscope slides do not need to be physically removed from one apparatus to another. Each treatment step occurs within the same reaction compartment. The reaction conditions of each reaction compartment for treating a slide can preferably be controlled independently, including the individualized application of reagents to each slide and the individualized treatment of each slide. The reagents are preferably held in a reagent dispensing strip similar to a “blister pack”. | 12-25-2008 |
20090011493 | DEVICE CONTAINING CYTOPHILIC ISLANDS THAT ADHERE CELLS SEPARATED BY CYTOPHOBIC REGIONS - The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM). | 01-08-2009 |
20090035844 | Mechanical cell wounder device and related method - Disclosed is a device and related method for biological manipulation. With greater particularity, disclosed is a novel device and method for uniform cellular manipulation with economies of scale and operational uniformity. The device and related method utilize a cellular wounder ( | 02-05-2009 |
20090075359 | Apparatus and method for encapsulating pancreatic cells - An apparatus and method for coating micron-sized or sub-micron-sized particles such as living cells. The coating apparatus includes an encapsulation chamber enclosing a two-layer water-oil system for coating each islet cell with an aqueous polymeric coat. Islets together with an aqueous polymer solution are fed by a feed device that utilizes the principle of hydrodynamic focusing in order to ensure encapsulation of individual islets. The polymer in the aqueous coat is subsequently crosslinked by being exposed to laser light to produce structurally stable microcapsules of controllable thickness of the order of tens of microns. Encapsulated islets are removed from the encapsulation chamber by a valveless pump and recovered by filtration or centrifugation. | 03-19-2009 |
20090098639 | APPARATUS FOR EXECUTION OF TREATMENT OPERATIONS IN CONNECTION WITH COLOURING OF TISSUE SPECIMENS ON OBJECT GLASSES - An apparatus for automatic execution of different treatment operations in connection with staining of tissue specimens on microscope slides, wherein the apparatus ( | 04-16-2009 |
20090098640 | METHOD AND APPARATUS FOR PREPARING CELLS FOR MICROTOME SECTIONING AND ARCHIVING NUCLEIC ACIDS AND PROTEINS - A method and apparatus for embedding cells that utilizes a flow-through embedding technique maximizes the efficiency of extractions and decreases time for embedding the cell fragments, minimizes cell loss, and automatically positions cell samples at the position in which a microtome blade will section them. The apparatus includes a cell flow pathway defined by an inflow tube for delivering cell fragments from a cell sample to a sample port. The sample port is in fluid communication with a tissue cassette having attached thereto a filter. The cell flow pathway is in communication with a reagent flow pathway for delivering the reagents through the sample port to the cassette. The apparatus is configured such that the application of pressure directs the cell fragments from the cell sample through the cell flow pathway, and effects delivery of the reagents through the reagent flow pathway. The apparatus produces an embedded cell block having concentrated cells near the plane of the block to be sectioned in a quick and efficient manner. | 04-16-2009 |
20090162925 | CELL/TISSUE MASS SELECTING APPARATUS AND DIVIDING MECHANISM THEREOF - A selecting apparatus for selecting cell/tissue mass includes a base, a feeding mechanism and a dividing mechanism. The base has a platform for placing the cell/tissue mass. The feeding mechanism, disposed on the base, moves relative to the platform. The dividing mechanism, disposed on the feeding mechanism, includes a first cutting set and a second cutting set connected therewith. The second cutting set reciprocally moves between a first position and a second position, toward the first cutting set. The feeding mechanism moves the dividing mechanism above the platform, aligning the dividing mechanism with a portion of the cell/tissue mass, and moves the first cutting set toward the cell/tissue mass, cutting into the portion of the cell/tissue mass. The second cutting set moves from the first position to the second position, limiting the portion of the cell/tissue mass between the first cutting set and the second cutting set. | 06-25-2009 |
20090233349 | Metal Nanowires With An Oxide Sheath And Production Method For Same - A one-dimensional composite structure which comprises at least one nanowire. The nanowire comprises a metal core and a metal oxide sheath. | 09-17-2009 |
20090286306 | SAMPLE CHAMBER VOLUME REDUCER - An apparatus includes a chamber insert capable of being received by a sample chamber of a conditioning system. The received chamber insert reduces the volume of the sample chamber and defines a cavity that substantially surrounds a sample holder located in the sample chamber. | 11-19-2009 |
20090325273 | MULTI-FUNCTIONAL TREATING DEVICE FOR TREATING PATHOLOGICAL TISSUE SECTIONS - A multi-functional treating device for pathological tissue sections includes a main body provided with at least one accommodating chamber formed in its top portion and a central controller. A water bath, an oven, a paraffin scrubber and a section baking rack are respectively installed in each of the accommodating chambers. The central controller is provided with plural control buttons, which are respectively connected with an electrothermal device installed under the paraffin scrubber, the water bath, the oven and the section baking rack respectively. Thus, the multi-functional treating device can simultaneously do jobs of scrubbing off extra paraffin, wetting tissue sections, primarily drying up tissue sections, and drying up slides carrying the sections, saving a lot of space. | 12-31-2009 |
20100009429 | IN SITU HEAT INDUCED ANTIGEN RECOVERY AND STAINING APPARATUS AND METHOD - An automated in situ heat induced antigen recovery and staining method and apparatus for treating a plurality of microscope slides. The process of heat induced antigen recovery and the process of staining the biological sample on the microscope slide are conducted in the same apparatus, wherein the microscope slides do not need to be physically removed from one apparatus to another. The reaction conditions for treating a slide can preferably be controlled independently, including the individualized application of reagents to each slide and the individualized treatment of each slide. | 01-14-2010 |
20100028978 | IN SITU HEAT INDUCED ANTIGEN RECOVERY AND STAINING APPARATUS AND METHOD - An automated microscope slide staining system and staining apparatus and method that features a plurality of individually operable miniaturized pressurizable reaction compartments or a pressurizable common chamber for individually and independently processing a plurality of microscope slides. The apparatus preferably features independently movable slide support elements each having an individually operable heating element. | 02-04-2010 |
20100068793 | DEVICES AND METHODS FOR PRODUCTION OF CELL AGGREGATES - A microwell device comprises a fluid vessel having a bottom region. A microwell plate is at the bottom region. The microwell plate comprises an upper region defining an upper plane. A plurality of microwells extend downwardly from the upper plane. Each of the microwells comprises an axis extending perpendicularly to the upper plane. Each of the microwells comprises a sidewall. The sidewall of at least one of the microwells has at least one wall component extending inwardly towards the axis. | 03-18-2010 |
20100093065 | SINGLE CELL MICROINJECTION USING FLEXIBLE AND COMPLIANT FLUIDIC CHANNELS AND ELECTROOSMOTIC DOSAGE CONTROL - A microinjection device for injecting a target cell with a reagent. The microinjection device includes a flexible substrate; a target supply channel formed in the flexible substrate for receiving the target cell; a reagent supply channel formed in the flexible substrate for receiving the reagent; a suction capillary mounted within a suction channel formed in the flexible substrate, the suction capillary providing suction to the target supply channel for immobilizing the target cell within the target supply channel; an injection needle mounted within a needle channel formed in the flexible substrate, the injection needle being movable between injected and un-injected positions by deforming at least a part of the flexible substrate; and a plurality of electrodes embedded in the flexible substrate, the plurality of electrodes creating a voltage potential across the injection needle to move the reagent into the target when the injection needle is in the injected position. | 04-15-2010 |
20100099171 | VITRIFICATION APPARATUS FOR MICRODROP VITRIFICATION OF CELLS AND A METHOD OF MICRODROP VITRIFICATION OF CELLS USING THE APPARATUS - An apparatus for microdrop vitrification has a tank and two wings. The tank has a bottom, two side edges, two top edges, two sidewalls, a lowest bottom, a drain and a spout. The drain is defined in one of the sidewalls at the bottom of the tank. The spout is mounted on the bottom of the tank and is connected to the drain. The wings are integrally formed respectively with and extend out from the top edges. The present invention also relates to a microdrop forming device, a method for microdrop vitrification and a method for recovering vitrified cells. | 04-22-2010 |
20100112677 | STERILE ENVIRONMENT MAINTAINING APPARATUS - Provided is a sterile environment maintaining apparatus capable of sterilizing a sterile room in a shorter time than conventional ones. | 05-06-2010 |
20100120130 | Droplet Actuator with Droplet Retention Structures - The present invention is directed to droplet actuators with droplet retention structures, and methods related thereto. In an exemplary embodiment, the invention provides a droplet actuator with one or more substrates arranged to form a droplet operations gap comprising gap-facing surfaces; droplet operations electrodes configured to conduct droplet operations in the droplet operations gap; at least one barrier included on at least one of the substrate surfaces and having dimensions selected to: permit droplet transport from atop a first droplet operations electrode to a second droplet operations electrode when the second droplet operations electrode is activated; and prevent movement of a droplet from atop a first droplet operations electrode when the first and second droplet operations electrodes are inactive. | 05-13-2010 |
20100136668 | SYSTEMS AND METHODS FOR ISOLATING AND USING CLINICALLY SAFE ADIPOSE DERIVED REGENERATIVE CELLS - Systems and methods are described that are used to separate cells from a wide variety of tissues. In particular, automated systems and methods are described that separate regenerative cells, e.g., stem and/or progenitor cells, from adipose tissue. The systems and methods described herein provide rapid and reliable methods of separating and concentrating regenerative cells suitable for re-infusion into a subject. | 06-03-2010 |
20100144018 | AUTOMATED SLIDE STAINING APPARATUS - A slide stainer assembly is disclosed. The slide stainer includes a slide carrier that is configured to carry one or more laboratory slides and a plurality of slide processing stations for processing the slides. A transport member including a plurality of engagement portions is configured for releasably engaging the slide carrier. A drive mechanism is coupled to the transport member. The drive mechanism is configured to move the transport member in a cyclical path such that, in the course of one cycle, the transport member engages a slide carrier docked in a first station, removes the slide carrier from the first station, docks the slide carrier in a second station and returns to the first station to engage another slide carrier. | 06-10-2010 |
20100167382 | METHOD AND DEVICE FOR THE FORMATION OF BIOLOGICAL CELL MATERIAL - A manipulation tool is disclosed for producing cell material having multiple biological cells, which have a predefined geometrical arrangement. The tool includes a tool body, whose surface at least partially contacts the cell material, and a setting device for adjusting the tool body by a continuous expansion, so that geometrical properties of the surface change and an interior of the tool body is enlarged. The setting device is adapted to expand the tool body at an advance velocity in a range from 0.1 μm/h to 1 mm/h. | 07-01-2010 |
20100216221 | DEVICE WITH WHICH A HISTOLOGICAL SECTION GENERATED ON A BLADE OF A MICROTOME CAN BE APPLIED TO A SLIDE - A device for applying a histological section to a slide is described. The histological section is generated by a cutting action performed by a blade of a microtome. The device comprises a positioning device having a component that is rotatably mounted to a bearing and has a receptacle for receiving and holding the slide, wherein the positioning device is designed such that the slide received in the receptacle can be rotated about an axis of rotation of the rotatably mounted component. | 08-26-2010 |
20100248338 | CELL TREATMENT DEVICE, CELL TREATMENT CARTRIDGE AND BODY FLUID TREATMENT SYSTEM - A cell treatment device which allows cell dispersion liquid including cancer cells to pass therethrough to cause at least the cancer cells to be subjected to at least one of a physical action, a chemical action, and a biologically activating action, includes: a slit member having a first surface and a second surface and including a through hole having a slit shape penetrating from the first surface to the second surface and extending in one direction, the through hole having a tapered portion, wherein the width of a cross section of the tapered portion orthogonal in the one direction reduces from the first surface toward the second surface side, and is smaller than an average diameter of the cancer cells at an end on the second surfaces side, and the cell dispersion liquid is allowed to pass from the first surface side through the through hole to the second surface side. | 09-30-2010 |
20100255563 | CONTAINER AND ANALYSIS CONTAINER USING THE SAME - A vessel, which can easily be opened and closed without detaching and attaching a cover, is provided. The vessel of the present invention includes a vessel body | 10-07-2010 |
20100261261 | CELL/TISSUE MASS SELECTING APPARATUS AND DIVIDING MECHANISM THEREOF - A selecting apparatus for selecting cell/tissue mass includes a base, a feeding mechanism and a dividing mechanism. The base has a platform for placing the cell/tissue mass. The feeding mechanism, disposed on the base, moves relative to the platform. The dividing mechanism, disposed on the feeding mechanism, includes a first cutting set and a second cutting set connected therewith. The second cutting set reciprocally moves between a first position and a second position, toward the first cutting set. The feeding mechanism moves the dividing mechanism above the platform, aligning the dividing mechanism with a portion of the cell/tissue mass, and moves the first cutting set toward the cell/tissue mass, cutting into the portion of the cell/tissue mass. The second cutting set moves from the first position to the second position, limiting the portion of the cell/tissue mass between the first cutting set and the second cutting set. | 10-14-2010 |
20100273243 | NOZZLE DEVICE AND LIQUID SAMPLE ANALYZER - A nozzle device which can perform accurate quantitative suction without being affected by a pressure when, for example, a nozzle ( | 10-28-2010 |
20100291662 | CONTAINER FOR THE PREPARATION, PRESERVATION AND STORAGE OF BIOLOGICAL SAMPLES USING A DRYING AGENT - The invention relates to a sample container ( | 11-18-2010 |
20100330659 | Tissue Processing Apparatus - An apparatus ( | 12-30-2010 |
20110020917 | NANOFIBERS CONTAINING LATENT REACTIVE GROUPS - A nanofiber is formed by combining one or more natural or synthetic polymeric materials and one or more than one cross-linking agents having at least two latent reactive activatable groups. The latent reactive activatable nanofiber may be used to modify the surface of a substrate by activating at least one of the latent reactive activatable groups to bond the nanofiber to the surface by the formation of a covalent bond between the surface of the substrate and the latent reactive activatable group. Some of the remaining latent reactive activatable group(s) are left accessible on the surface of the substrate, and may be used for further surface modification of the substrate. Biologically active materials may be immobilized on the nanofiber modified surface by reacting with the latent reactive groups that are accessible on the surface of the substrate. | 01-27-2011 |
20110027871 | Apparatus for Demineralizing Osteoinductive Bone - The invention is directed to an apparatus for producing demineralized osteoinductive bone. The apparatus demineralizes bone by subjecting bone, including, for example, ground bone, bone cubes, chips, strips, or essentially intact bone, to either a rapid high volume pulsatile acidification wave process or to a rapid continuous acid demineralization process. The pulsatile acidification wave process includes subjecting bone to two or more rapid pulse/drain cycles in which one or more demineralizing acids is rapidly pulsed into a vessel containing bone, and after a desired period of time, is rapidly drained from the vessel. The continuous acid demineralization process includes subjecting bone to a continuous exchange of demineralizing acid solution in which the demineralizing acid solution is recirculated from the container holding the bone through an ion exchange media. Calcium and phosphate are thereby removed from the bone to produce a regenerated acid, and the regenerated acid is returned to the container holding the bone. Both processes allow bone to be rapidly demineralized to a precise and specific desired residual calcium level without sacrificing osteoinductivity. | 02-03-2011 |
20110076753 | Apparatus for Embedding Tissue Samples - A histology tissue embedding apparatus including a reservoir for an embedding medium and an outlet valve connected to the reservoir through which the embedding medium is dispensable, and including trigger means which control the operation of the outlet valve, the trigger means having a rest position in which the outlet valve is closed, wherein the rest position of the trigger means in relation to the outlet valve is adjustable. | 03-31-2011 |
20110076754 | DEVICE AND METHOD FOR FILTERING ONE OR MORE PARTICLES TO BE DETECTED FROM A FLUID - A device is disclosed for filtering a particle to be detected from a fluid. In at least one embodiment, the device includes a substrate and, applied above it, a protective layer, a channel being disposed between them for the purpose of receiving a fluid containing different particles. In at least one embodiment, the channel extends at least partially in a spiral shape between its inlet and outlet and on its internal wall contains a coating for attracting a particle type to be filtered, which coating is applied at least on the radially outside subsection of the internal wall of the channel. Owing to the laminar longitudinal movement of the fluid inside the channel a radially outward directed centrifugal force acts on the particles, with the result that per time unit more particles come into contact with the radially outside subsection of the internal wall of the channel than the radially inside subsection of the internal wall of the channel. The filter effect is reinforced as a result. | 03-31-2011 |
20110091961 | Tissue Processor For Treating Tissue Samples - A tissue processor for treating tissue samples comprises a process chamber ( | 04-21-2011 |
20110097787 | CONSTANT-TEMPERATURE EQUIPMENT - Constant-temperature equipment wherein maintenance is facilitated with the least failure, and highly reliable culturing and testing can be carried out. Mechanical and electrical structures are eliminated from the inside of a temperature-controlled chamber ( | 04-28-2011 |
20110104793 | DEVICE FOR APPLYING ELECTROMAGNETIC ENERGY TO A REACTIVE MEDIUM - The invention relates to a device ( | 05-05-2011 |
20110117633 | STRIPPING ABSORPTION MODULE - In a process, a portion of a liquid mixture flow is vaporized to produce a vapor and a depleted flow of liquid. The vapor is introduced to a brine which is adapted to exothermically absorb one or more components therefrom, and heat is withdrawn, to produce at least a flow of heat and a flow of brine which is enriched in the one or more components. The heat previously withdrawn is transferred, to drive the vaporization. This transfer can be associated with the change of a working fluid from a gaseous into a liquid state. In this case, the heat withdrawal involves the change of the working fluid from the liquid to the gaseous state. In the liquid state, the working fluid flows only by one or more of gravity, convection and wicking. In the gaseous state, the working fluid flows only by one or more of diffusion and convection. | 05-19-2011 |
20110117634 | FLAT CELL CARRIERS WITH CELL TRAPS - Cell carrier structures using dynamic flow of cell bearing, washing, or nourishing fluid, from an input reservoir region to an output reservoir region. A common feature of the several structures by which this can be achieved is the presence of channels generally having low height or other cross section, such that the cell bearing fluid readily traverses these channels by capillary action. Optional pumping assistance can also be provided. The capture wells or traps are disposed generally along the length of these channels such that the cells have multiple chances of being captured in a trap or well. The traps or wells are structured such that only a single cell can be trapped in each well or trap, and the disposition of the wells or traps as appendages to the fluid flow channels facilitates the washing or nourishing of the cells while their proliferation or development is being observed. | 05-19-2011 |
20110117635 | Multipurpose Micro Electric Field Network Cell Processing Device - A multipurpose micro electric field network cell processing device comprises: a hollow frame structure; transparent film layers which are respectively correspondingly arranged on the upper side surface and the lower side surface of the frame; electrode groups which are arranged on the transparent film layer on at least one side and used for generating a low strength electric field; wire connecting terminals of the electrode groups arranged on at least one end of the frame; and an inlet and an outlet of culture/processing solution arranged at two corresponding ends of the frame, wherein sandwich space surrounded by the two transparent film layers and the frame constitutes a cell culture/processing chamber. The device can effectively introduce or deliver a drug into target cells under a low strength electric field network environment, monitor and control the whole experiment/processing process more precisely and be conducive to separation and selection of the target cells. | 05-19-2011 |
20110124093 | CONSTANT-TEMPERATURE EQUIPMENT - Provided is constant-temperature equipment wherein maintenance is facilitated with the least failure, and highly reliable culturing and testing can be carried out. Mechanical and electrical structures are eliminated from the inside of a temperature-controlled chamber ( | 05-26-2011 |
20110136214 | DUST ARRESTER AND METHOD FOR REMOVING DUST PARTICLES FROM AIR - A dust arrester includes a housing with air inlet and air outlet, wherein the ambient air containing dust particles can be introduced into the housing via the air inlet and discharged from the housing via the air outlet. The dust arrester further includes a water trough placed in the bottom of the housing and a chaos effect generator including a nozzle and a material. The nozzle can spray water supplied from the water trough. The water sprayed interacts with the material to generate a chaos condition, so that the dust particles can be removed from the introduced air under the chaos condition. | 06-09-2011 |
20110183405 | MODIFIED MULTIWELL PLATE FOR BIOCHEMICAL ANALYSES AND CELL CULTURE EXPERIMENTS. - The invention relates to a modified multi-well plate for biochemical analyses and cell culture experiments, which can be obtained through a method for functionalization. The method comprises the following process steps:
| 07-28-2011 |
20110183406 | Apparatus For Separating Tissue Cells From A Fluid - The invention relates to an apparatus for separating tissue cells from a fluid, with a collection container forming a collection space, which is connected, on one hand, with a negative pressure source and which is connected, on the other hand, with a supply line for the fluid-tissue cell mixture. | 07-28-2011 |
20110189761 | METHOD FOR PRODUCING CELL CULTURE SCAFFOLD - To provide a simple method for producing a cell culture scaffold material having a nanoporous surface in which the pore density, pore diameter and pore diameter distribution are easily controlled. | 08-04-2011 |
20110195487 | SAMPLE CONTAINER FOR BIOMARKER MAINTENANCE - Sample container for biomarker maintenance. In at least one embodiment of a system for stabilizing a diagnostic marker of the present disclosure, the system comprises a sample container having an opening and least one rigid chamber for containing a body fluid, the body fluid comprising at least one diagnostic marker, a stabilizing agent contained within the sample container, the stabilizing agent capable of completely or substantially preventing the degradation or inactivation of the diagnostic marker, and a securing device for closing the opening of the sample container, wherein the stabilizing agent is coupled to a solid support. In an exemplary embodiment of a system of the present disclosure, the stabilizing agent may be adsorbed to the solid support. | 08-11-2011 |
20110195488 | DEVICES FOR COLLECTION AND STABILIZATION OF BIOMARKERS IN LIQUID SAMPLES - Device for collection and stabilization of biomarkers in liquid samples. In at least one embodiment of a fluid collection device, the device comprises a first tube operable to receive a body fluid, a second tube operable to dispense a body fluid, and a suction device operably connected to the second opening of the first tube and the first end of the second tube, wherein the suction device is operable to produce negative pressure through the first tube and is operable to exert positive pressure through the second tube. An embodiment of the devices also comprises a stabilizing agent contained within the fluid collection device, the stabilizing agent capable of completely or substantially preventing the degradation or inactivation of a diagnostic agent in a body fluid upon receipt of the body fluid into the fluid collection device. | 08-11-2011 |
20110195489 | System and Method of an In-vitro Wound Healing Model on Cultured Cell Monolayer Employing Stamp Device - A system and method of an in vitro wounding model that employs a wounding device compromising a stamp assembly with a stamp disk engraved with alternating grooves and ridges, to dislodge cells of cultured cell monolayer. Strips or islands of cells are left behind, which can be used for morphological observation in biological research. Further and more importantly, these strips or islands of cells can be designed to be sufficiently narrow or small (˜100 μm) to ensure that all remaining cells become rapidly activated and then migrate, dedifferentiate and proliferate in near synchrony, so that samples can be collected in large amount which is suitable for biochemical studies. | 08-11-2011 |
20110201097 | Ionic Liquid Apparatus and Method for Biological Samples - Apparatus and method for handling biological samples. Segments of ionic liquid can provide voltage across segments of immiscible liquid to concentrate or separate charged species in the biological samples. Reactants in biological samples can be contacted and reacted in segments of immiscible liquid. | 08-18-2011 |
20110212509 | Device For And Method Of Extracting A Fraction From A Biological Sample - A device and method are provided for facilitating extraction of a fraction from a biological sample. The biological sample includes non-desired material and a fraction-bound solid phase substrate. The device includes an input zone for receiving the biological sample therein and a phase-gate zone for receiving an isolation buffer therein. An output zone receives a reagent therein. A force is movable between a first position adjacent the input zone and a second position adjacent the output zone. The force urges the fraction-bound solid phase substrate from the input zone, through the phase-gate zone and into the output zone. | 09-01-2011 |
20110236959 | METHOD OF MANUFACTURING PLANAR BIO-TEST STRIP AND PRODUCT THEREOF - A planar bio-test strip includes a carrier plate, a conductive circuit formed on the carrier plate by evaporation, a testing electrode terminal and an electrode circuit terminal formed at front and rear ends of the conductive circuit respectively, and an enzyme reacting area coupled to a front end of the testing electrode terminal. A manufacturing method of the planar bio-test strip includes (1) using a plastic film as a carrier plate, and constructing a shadow mask by printing; (2) evaporating the carrier plate printed with the shadow mask to form a conductive electroplated layer; (3) removing a block of the conductive electroplated layer covered by the shadow mask by a physical method to expose the conductive circuit; (4) setting an enzyme in an enzyme reacting area; (5) attaching a cover film; such that the test strip can be mass produced with low costs, and enhanced quality, stability and market competiveness. | 09-29-2011 |
20110236960 | APPARATUS AND METHODS FOR INTEGRATED SAMPLE PREPARATION, REACTION AND DETECTION - Cartridges for the isolation of a biological sample and downstream biological assays on the sample are provided. In one embodiment, a nucleic acid sample is isolated from a biological sample and the nucleic acid sample is amplified, for example by the polymerase chain reaction. The cartridges provided herein can also be used for the isolation of non-nucleic acid samples, for example proteins, and to perform downstream reactions on the proteins, for example, binding assays. Instruments for carrying out the downstream biological assays and for detecting the results of the assays are also provided. | 09-29-2011 |
20110263005 | PIPETTE TIP FOR ELECTROPORATION DEVICE - Provided herein is a pipette tip ( | 10-27-2011 |
20110300609 | NUCLEIC ACID EXTRACTION APPARATUS - The present invention relates to a nucleic acid extracting apparatus, and the nucleic acid extracting apparatus can include a pipe-shaped tube having an open outlet at one side thereof, and a hydrogel supporting member that is provided inside the tube and filters impurities excluding an extraction target material. | 12-08-2011 |
20110300610 | Thermally Conductive Microplate - Embodiments according to the present teachings of a microplate comprising a main body portion having a first surface and an opposing second surface are disclosed. A plurality of wells are formed in the first surface, each of the plurality of wells being sized to receive an assay therein. A backing is coupled to the opposing second surface of the main body portion. | 12-08-2011 |
20110312067 | DIALYSIS DEVICE FOR SEPARATING PATHOGENS FROM A BIOLOGICAL SAMPLE - A dialysis device to separate pathogens from a biological sample, the dialysis device having a first channel for receiving the biological sample, a second channel, and, a plurality of apertures, wherein, the second channel is fluidically connected to the first channel via the apertures such that the pathogens flow from the first channel to the second channel and larger constituents in the biological sample remain in the first channel. | 12-22-2011 |
20110312068 | DIALYSIS DEVICE FOR SEPARATING NUCLEATED CELLS IN A BIOLOGICAL SAMPLE FROM OTHER CONSTITUENTS - A dialysis device to separate nucleated cells in a biological sample from smaller constituents, the dialysis device having a first channel for receiving the biological sample, a second channel, and, a plurality of apertures, wherein, the second channel is fluidically connected to the first channel via the apertures, the apertures being smaller than the nucleated cells such that the smaller constituents flow from the first channel to the second channel and the nucleated cells in the biological sample remain in the first channel | 12-22-2011 |
20110312069 | MICROVIAL WITH DIGITAL MEMORY FOR STORAGE OF OLIGONUCLEOTIDE SPECIFICATION DATA - An oligonucleotide microvial for an oligonucleotide dispensing apparatus, the microvial having a container for holding a volume of reagent, an integrated circuit with digital memory storing oligonucleotide data relating to specifications characterizing the oligonucleotides, and, electrical contacts for connection to a control processor in the reagent dispensing apparatus to provide the data to the control processor for download into devices supplied with the oligonucleotides. | 12-22-2011 |
20120003725 | MICROFLUIDIC CHIP FOR ANALYSIS FOR FLUID SAMPLE - Disclosed is a microfluidic chip capable of accurately and quickly detecting presence of a trace amount of a target within a fluid sample. It has a channel structure comprising a sample inlet for feeding a sample therethrough, a first reservoir for primarily storing the sample therein, a second reservoir for secondarily storing the sample therein, a first reaction portion in which a target is conjugated with a label, a second reaction portion in which the labeled target undergoes a specific reaction, such as an antigen-antibody reaction, and a delaying portion, located between the first and the second reaction portion, for decreasing a flow rate of the sample. | 01-05-2012 |
20120009662 | METHOD OF FABRICATING MICROFLUIDIC SYSTEMS - A method of fabricating a microfluidic system having microfluidic channels on a surface of a hydrophilic substrate, the method including the steps of: hydrophobizing the substrate surface; locating a mask defining the substrate surface, the mask having open areas defining the periphery of the microfluidic channels; and applying an irradiation treatment to areas of the substrate surface exposed by the open areas of the mask, said exposed areas becoming hydrophilic to therefore form said microfluidic channels. | 01-12-2012 |
20120015428 | METERING ROTARY NANOPUMP, METHOD OF FABRICATING SAME, AND APPLICATIONS OF SAME - In one aspect of the present invention, a pump includes a cam having a cam shaft, wherein the cam shaft has an axis, an exterior surface and M fins spaced-apart formed on the exterior surface along the axis; and at least one fluidic flow channel having an inlet and an outlet, disposed on a substrate, wherein the at least one fluidic flow channel helically surrounds the cam shaft such that when the cam shaft driven by the cam rotates, the M fins of the cam shaft compresses the at least one fluidic flow channels to produce a wave of compression that actuates a peristaltic flow of fluid therein so as to direct a desired amount of the fluid toward one of the inlet and the outlet. | 01-19-2012 |
20120021501 | Cell Separation Method And Apparatus - Disclosed herein are apparatus and methods for isolating a fraction of interest from a physiological fluid sample. A sample holder for isolating a fraction of interest from a physiological fluid sample includes a flexible compartment and a rigid exoskeleton that supports the flexible compartment. The flexible compartment may have at least one reservoir with a height to volume ratio of about 0.1 cm/mL to about 5 cm/mL. An automated device for extracting a fraction of interest from the sample includes a sample holder with a flexible compartment supported by a rigid exoskeleton, a support for the sample holder connected to one or more fluid extraction devices, and a motor for moving the extraction device relative to the sample holder. The automated device may include an optical sensor and may include a clamp for clamping the flexible compartment. | 01-26-2012 |
20120028342 | SLIP CHIP DEVICE AND METHODS - A device is described having a first surface having a plurality of first areas and a second surface having a plurality of second areas. The first surface and the second surface are opposed to one another and can move relative to each other from at least a first position where none of the plurality of first areas, having a first substance, are exposed to plurality of second areas, having a second substance, to a second position. When in the second position, the plurality of first and second areas, and therefore the first and second substances, are exposed to one another. The device may further include a series of ducts in communication with a plurality of first second areas to allow for a substance to be disposed in, or upon, the plurality of second areas when in the first position. | 02-02-2012 |
20120040444 | METHOD AND APPARATUS FOR PREPARING CELLS FOR MICROTOME SECTIONING AND ARCHIVING NUCLEIC ACIDS AND PROTEINS - A method and apparatus for embedding cells that utilizes a flow-through embedding technique. The apparatus includes a cell flow pathway defined by an inflow tube for delivering cell fragments from a cell sample to a sample port. The sample port is in fluid communication with a tissue cassette having attached thereto a filter. The cell flow pathway is in communication with a reagent flow pathway for delivering the reagents through the sample port to the cassette. The apparatus is configured such that the application of pressure directs the cell fragments from the cell sample through the cell flow pathway, and effects delivery of the reagents through the reagent flow pathway. The apparatus produces an embedded cell block having concentrated cells near the plane of the block to be sectioned in a quick and efficient manner. | 02-16-2012 |
20120045823 | Method and Apparatus for Collecting Biological Materials - A method and apparatus can separate and concentrate a selected component from a multi-component material. The multi-component material may include a whole sample such as adipose tissue, whole blood, or the like. The apparatus generally includes a moveable piston positioned within a separation container and a withdrawal tube that is operable to interact with a distal end of the collection container past the piston. Material can be withdrawn through the withdrawal tube. | 02-23-2012 |
20120070883 | HIGH TEMPERATURE HIGH PRESSURE MICROBIAL REACTOR - The high temperature/high pressure (HTHP) reactor of the present invention includes a main cylinder, a reversible piston inside the cylinder that divides the main cylinder into a pressurization chamber and a reactor chamber, a thermal jacket, a lid and an end cap. The HTHP reactor of the present invention is configured to facilitate microbial growth in the reactor chamber that provides high temperature and high pressure conditions that simulate resource reservoir conditions, wherein the resource may be an oil reservoir. The HTHP chamber of the present invention is configured to receive a fluid sample from the underground oil reservoir that has been maintained at reservoir temperature and pressure throughout sampling, transfer to transport bottle, transport to the laboratory, and inoculation into the chamber. The HTHP chamber of the present invention is also configured to allow the use of a variety of instrumentation and valves that can be customized to allow a user to monitor desired physical properties within the reaction chamber and the microbial behavior and byproducts. This information is used to create an individual treatment plan for a reservoir to maximize the resource recovery, for example during Microbiobial Enhanced Oil Recovery (MEOR). | 03-22-2012 |
20120094364 | CONTINUOUS COUNTERCURRENT FLUIDIZED MOVING BED (FMB) AND/OR EXPANDED MOVING BED (EMB) - The composite continuous countercurrent fluidized moving bed (FMB) and/or expanded moving bed (EMB) for carrying out processes such as recovery, purification or reaction of single or multiple component/s of interest, by contacting liquid phase containing the said component/s with the solid adsorbent in continuous countercurrent mode. In this composite fluidized moving bed and/or expanded moving bed system the net movement of the solids is against the liquid flowing in upward direction through the stages/columns, and sedimenting solids from the one stage/column are continuously fed to the top of the another stage/column placed below or alongside of previous stage/column, and also operating in fluidized/expanded bed mode, wherein its countercurrent contact with up flowing liquid is carried out. The system described herein consists of number of stages/column as per requirement of the process. The sedimenting adsorbent solids from the last fluidized bed or expanded bed stage/column are taken out continuously and fed back to the top of the first stage/column, through a feed hopper to which the adsorbent solids are transported by means of pump or liquid solid ejector, or any other suitable device. The feed hopper is suitably designed and operated, so as deliver solid adsorbents to the top of the first stage/column continuously in a controlled manner. The flow of solid adsorbent through the said solid downcomer tube/s is controlled using valves appropriate to afford control over the downward adsorbent flow. The FMB/EMB system can be used in processes for continuous recovery, purifications or reactions of various products. | 04-19-2012 |
20120094365 | MOLECULAR MOTOR - A molecular motor in which multiple concentric cylinders (or nested cones) rotate around a common longitudinal axis. Opposing complementary surfaces of the cylinders or cones are coated with complementary motor protein pairs (such as actin and myosin). The actin and myosin interact with one another in the presence of ATP to rotate the cylinders or cones relative to one another, and this rotational energy is harnessed to produce work. The length of the cylinders can also be used to control the power generated by the motor. In another embodiment, the molecular motor includes at least two annular substrates wherein one annular substrate is coated with a first motor protein and the other annular substrate is coated with a second motor protein. The first and second motor proteins interact with each other to move the second annular relative to the first annular substrate. | 04-19-2012 |
20120122197 | INKJET REAGENT DEPOSITION FOR BIOSENSOR MANUFACTURING - A technique for producing a biosensor includes inkjet printing a reagent onto electrodes of the biosensor. The ink has been specially formulated to allow the reagent to be printed using inkjet printing while at the same time produce commercially viable biosensor. The inkjet printing of the reagent allows for different inkjet patterns to be produced as well as facilitates quick change over between various products. For example, the technique allows the reagent and electrode to be formed on opposite sides of a substrate. In another example, the reagent can be layered such that incompatible reagents can be separated by a barrier layer. The electrodes for the biosensor can also be inkjet printed such that most of the biosensor can be produced using inkjet technology. | 05-17-2012 |
20120164715 | DEVICE FOR PORCESSING A CYTOLOGICAL OR HISTOLOGICAL PREPARATION - A device for processing a cytological or histological preparation includes: a carousel, including at least one space for receiving a slide for receiving a cytological or histological preparation, the carousel being rotatably mounted about an axis of rotation; a nebulizing head that passes above the carousel, including the piezoelectric element and a space for the reagent such that the nebulising head expels at least one drop of reagent as a result of the deformation of the piezoelectric element. | 06-28-2012 |
20120171755 | FABRICATION OF HOLLOW NANONEEDLES - The present invention provides a method of fabricating a device having hollow nanoneedles which extend through a supporting membrane. The device can be used as a molecular delivery system. | 07-05-2012 |
20120190103 | DEVICE FOR THE TREATMENT OF BIOLOGICAL FLUID - The invention relates to a device ( | 07-26-2012 |
20120214222 | METHOD FOR MANUFACTURING A DEVICE FOR REGENERATING BIOLOGICAL TISSUES, PARTICULARLY FOR REGENERATING TISSUES OF THE CENTRAL NERVOUS SYSTEM - A method for manufacturing a device for regenerating biological tissues, particularly for regenerating tissues of the central nervous system, and a device that can be manufactured with said method, the device comprising an outer sheath based on collagen which is substantially tubular and can be interposed between the endings of a biological tissue to be regenerated, and at least one supporting element based on collagen, which is accommodated inside the outer sheath. | 08-23-2012 |
20120225473 | Devices And Method For Enrichment And Alteration Of Cells And Other Particles - The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample. | 09-06-2012 |
20120231529 | FLUID CONCENTRATOR WITH REMOVABLE CARTRIDGE - A fluid concentrator includes a main housing and a cartridge removably engaged to the main housing. The main housing includes a first end portion having a first port and a second port, an oppositely disposed second end portion and a separation chamber that extends between the first and second end portions. The second end portion has an inlet port and defines a cartridge passage that extends through the second end portion. The separation chamber is in fluid communication with the inlet port. The cartridge includes a first axial end portion that is engaged with the first end portion and an oppositely disposed second axial end portion, a portion of the second axial end portion is disposed in the cartridge passage of the second end portion of the main housing. The cartridge is adapted to filter fluid from the separation chamber. | 09-13-2012 |
20120231530 | METHODS OF CHROMOSOME DRYING AND SPREADING - The present invention provides for a method of drying and spreading chromosomes from various biological samples to yield optimal chromosomal spreading. The method requires preparing a biological sample for treatment, providing a cytogenetic chamber capable of setting predetermined conditions, pre-testing a portion of the biological sample in the cytogenetic chamber, and finally treating the remaining biological sample. The method is useful to yield metaphase chromosomes that are small and rounded, with very few overlapping or scattered chromosomes. Furthermore, the method is uses restricted ranges of temperature and relative humidity to achieve consistent chromosomal spreading. The morphologies of the chromosomes are preserved in order to execute banding techniques at 550 bands and chromosomal analysis on high-resolution chromosomes. | 09-13-2012 |
20120264200 | SYSTEMS AND METHODS FOR ISOLATING AND USING CLINICALLY SAFE ADIPOSE DERIVED REGENERATIVE CELLS - Systems and methods are described that are used to separate cells from a wide variety of tissues. In particular, automated systems and methods are described that separate regenerative cells, e.g., stem and/or progenitor cells, from adipose tissue. The systems and methods described herein provide rapid and reliable methods of separating and concentrating regenerative cells suitable for re-infusion into a subject. | 10-18-2012 |
20120322138 | SELECTIVE CYTOPHERESIS DEVICES AND RELATED METHODS THEREOF - The present invention relates to systems and devices to treat and/or prevent inflammatory conditions within a subject and to related methods. More particularly, the invention relates to systems, devices, and related methods that sequester leukocytes and/or platelets and then inhibit their inflammatory action. | 12-20-2012 |
20130005023 | APPARATUS AND METHOD FOR PROCESSING BIOLOGICAL MATERIAL - The application discloses an apparatus and method for processing biological material, including a suspension of cells. | 01-03-2013 |
20130005024 | APPARATUS AND METHODS FOR PROCESSING TISSUE TO RELEASE CELLS - An apparatus and methods for processing tissue to release biological material including cells are disclosed. | 01-03-2013 |
20130084627 | BIOCHIP - There is provided a biochip including a first substrate having micro wells formed to a predetermined depth and partitioned by a plurality of barrier ribs, and a reservoir formed below the micro wells and receiving liquid; and a second substrate coupled with the first substrate to thereby allow biomaterials provided thereon to be inserted into the micro wells while having a predetermined interval therebetween. | 04-04-2013 |
20130109080 | INTEGRATED SYSTEMS AND ASSEMBLIES FOR SAMPLE PROCESSING | 05-02-2013 |
20130171722 | METHOD AND APPARATUS FOR DELIVERY OF MOLECULES TO CELLS - The present invention is concerned with an apparatus for injection of a substance into a subject. The apparatus has an assembly including a plurality of elongate non-hollow micro-nanoneedles for delivering the substance into the subject, at least some of the micro-nanoneedles have a non-uniform diameter with a wider upper end and a narrower lower end and a length from 5-100 um, wherein the upper end has a diameter from 1-20 um and the lower end has a diameter from 50-400 nm, wherein the micro-nanoneedles are spaced apart with appropriate spacing therebetween such that when the micro-nanoneedles are penetrating cells in a penetration exercise each of the micro-nanoneedles may penetrate one cell only, and wherein the micro-nanoneedles are made from a relatively rigid material allowing the micro-nanoneedles to maintain rigidity during penetration and are configured to allow coating of the substance on the surface of the lower end. | 07-04-2013 |
20130189766 | NANOPARTICLE-TEXTURED SURFACES AND RELATED METHODS FOR SELECTIVE ADHESION, SENSING AND SEPARATION - Textured heterogeneous surfaces and related articles as can be used in conjunction with methods for selective sensing and/or separation. | 07-25-2013 |
20130217100 | Apparatus for Embedding Tissue Samples - A histology tissue embedding apparatus including a reservoir for an embedding medium and an outlet valve connected to the reservoir through which the embedding medium is dispensable, and including trigger means which control the operation of the outlet valve, the trigger means having a rest position in which the outlet valve is closed, wherein the rest position of the trigger means in relation to the outlet valve is adjustable. | 08-22-2013 |
20130230905 | Apparatus for Embedding Tissue Samples - A histology tissue embedding apparatus including a reservoir for an-embedding medium and an outlet valve connected to the reservoir through which the embedding medium is dispensable, and including trigger means which control the operation of the outlet valve, the trigger means having a rest position in which the outlet valve is closed, wherein the rest position of the trigger means in relation to the outlet valve is adjustable. | 09-05-2013 |
20130230906 | MICROFLUID CARTRIDGE FOR MOLECULAR DIAGNOSTICS - A cartridge for carrying out a method of analyzing the nucleic acids contained in a sample, includes a main body ( | 09-05-2013 |
20130252318 | Device for performing micro-operations on a vesicular object - A device for performing micro-operations on a vesicular target, comprising an injection pipette ( | 09-26-2013 |
20130288343 | BIOLOGICAL SAMPLE HOLDER AND METHOD OF ASSEMBLING A STORAGE DEVICE - Embodiments of the present invention, disclose a biological sample holder comprising a stake of biologically inert material, and a biological sample storage medium fixed to or retained on the stake by a retaining portion. This provides a means of holding a biological sample which is easy to handle and suitable for automation, for example in an array of such holders, allowing processing of multiple biological samples in parallel. | 10-31-2013 |
20130295653 | INTEGRATED ACTIVE FLUX MICROFLUIDIC DEVICES AND METHODS - The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. | 11-07-2013 |
20130330811 | THERMAL CYCLER - A thermal cycler includes: a fitting section for fitting a biochip; a first temperature setting section that sets the temperature of a first region of the biochip; a second temperature setting section that sets the temperature of a second region different from the first region to a temperature different from the first temperature setting section; a driving mechanism that changes a positional relationship between the first region and the second region with respect to the gravitational direction; and a control section that controls the driving mechanism, wherein the control section performs: a process of controlling the driving mechanism such that a first arrangement in which the first region is on the lower side of the second region with respect to the gravitational direction is kept for a first predetermined period. | 12-12-2013 |
20130337551 | APPARATUS AND METHODS FOR CELL ISOLATION - A unitary apparatus for isolating cells from adipose tissue including a lipid separation processor with a dispersing head equipped with a plurality of ports and a digestion chamber for dissociation of the constituent cells disposed in adipose tissue. The lipid separating apparatus is useful for the separation of lipids and adipocytes from a mixed cell population. A cell seeding chamber may be attached to the cell isolation apparatus. The components of the apparatus may be packaged in modular kit form. | 12-19-2013 |
20140038271 | METHODS FOR CANCER DETECTION, DIAGNOSIS AND PROGNOSIS - The present invention provides a method for diagnosing cancer, predicting a disease outcome or response to therapy in a patient sample. The method involves isolating a circulating tumor cell (CTC), for example, a viable CTC, from a sample using a parylene microfilter device comprising a membrane filter having or consisting of a parylene substrate, which has an array of holes with a predetermined shape and size; and detecting and quantifying telomerase activity in blood circulating tumor cells. The invention further provides methods of using cells live-captured in various applications. | 02-06-2014 |
20140087450 | DEPOSITION DEVICE, AND COLLECTION DEVICE | 03-27-2014 |
20140113356 | NANO-ELECTRODE BASED CHIP - The present invention disclosed a nano-electrode based transparent chip, which is applicable to perform a micro fluidic substance diffused through a specific region of a single cell membrane by localized electroporation technique with membrane reversibility. The chip comprises a silicon-based layer, different structural layers, an insulating layer and a micro fluidic layer. When the individual single cell was placed on the gap between a plurality of triangular-shaped nano-electrodes with nano-tip, then electric field can intense on a specific region of the individual single cell causes the formation of nano-pores on the membrane, resulting to deliver drugs, DNA molecules from outside of the cell to the inside of the cell with a high transfection rate and a high cell viability. This technique not only generates well-controlled nano-pores to allow rapid recovery of the cell membrane, but also provides clear optical path for potentially monitoring/tracing the drugs into the cell. | 04-24-2014 |
20140220665 | DYNAMIC MIXING AND ELECTROPORATION CHAMBER AND SYSTEM - The present invention relates to an apparatus for mixing cells and exogenous material for delivery of the exogenous material into cells using electroporation in a manner that preserves cell viability and integrity of the exogenous material. The exogenous material can be polynucleotides, peptides, proteins or other pharmaceutical molecules. It further provides a chamber that can be used to electroporate the contents of the chamber after mixing. In accordance with the invention, cell suspensions that may be contaminated with degrading enzymes are stored separately from water-based liquids that contain the exogenous material. Contact of the two water-based liquids (cell suspension and exogenous material) first occurs within the chamber. The chamber of the present invention has two or more inlet ports to allow separate entrance of water-based liquids. Two other ports are vent and exit ports. The inside of the chamber allows flow of all water-based liquids down the same side of the chamber and curved corners for redirecting flow direction and gently mixing water-based liquids. The chamber also includes electrodes as chamber walls for electroporation of chamber contents. | 08-07-2014 |
20140234948 | MOLECULAR MOTOR - A molecular motor in which multiple concentric cylinders (or nested cones) rotate around a common longitudinal axis. Opposing complementary surfaces of the cylinders or cones are coated with complementary motor protein pairs (such as actin and myosin). The actin and myosin interact with one another in the presence of ATP to rotate the cylinders or cones relative to one another, and this rotational energy is harnessed to produce work. The concentration of ATP and the number of nested cylinders or cones can be used to control the rotational speed of the motor. The length of the cylinders can also be used to control the power generated by the motor. In another embodiment, the molecular motor includes at least two annular substrates wherein one annular substrate is coated with a first motor protein and the other annular substrate is coated with a second motor protein. The first and second motor proteins interact with each other to move the second annular relative to the first annular substrate. | 08-21-2014 |
20150017709 | METHOD FOR DETECTING THE PRESENCE OF A TARGET NUCLEIC ACID SEQUENCE IN A SAMPLE - A method comprises loading a sample portion into a sample chamber which comprises means for minimizing diffusion of the sample portion, subjecting the sample portion to an amplification step, and determining whether the sample portion contains at least one molecule of a target nucleic acid. If the sample portion contains a single molecule of the target nucleic acid, the sample portion would attain a detectable concentration of the target nucleic acid after a single round of amplification. Also, a microfluidic device comprising a sample portion and a sample chamber comprising means for minimizing diffusion of the sample portion. Also, a microfluidic device comprising a sample chamber and an amplification targeting reagent positioned in the first sample chamber. | 01-15-2015 |
20150031116 | INTEGRATED ACTIVE FLUX MICROFLUIDIC DEVICES AND METHODS - The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules. These hybridization signals can be detected by any suitable means, for example optical, and can be stored for example in a computer as a representation of the presence of a particular gene. Hybridization probes can be immobilized on a substrate that forms part of or is exposed to a channel or channels of the device that form a closed loop, for circulation of sample to actively contact complementary probes. Universal chips according to the invention can be fabricated not only with DNA but also with other molecules such as RNA, proteins, peptide nucleic acid (PNA) and polyamide molecules. | 01-29-2015 |
20150093809 | Apparatus for enhanced recovery of regenerative cells from tissue sample - This document describes methods and an apparatus for recovery of a cell-enriched matrix and cells (e.g., regenerative cells) from a tissue sample. In some embodiments, at least two rounds of acceleration and deceleration are performed. | 04-02-2015 |
20150093810 | Particle manipulation system with out-of-plane channel and focusing element - A particle manipulation system uses a MEMS-based, microfabricated particle manipulation device, which has an inlet channel, output channels, and a movable member formed on a substrate. The movable member moves parallel to the fabrication plane, as does fluid flowing in the inlet channel. The movable member separates a target particle from the rest of the particles, diverting it into an output channel. However, at least one output channel is not parallel to the fabrication plane. The device may be used to separate a target particle from non-target material in a sample stream. The target particle may be, for example, a stern cell, zygote, a cancer cell, a component of blood, bacteria or DNA sample, for example. The particle manipulation system may also include a microfluidic structure which focuses the target particles in a particular portion of the inlet channel. | 04-02-2015 |
20150093811 | MIXING DEVICE FOR A CONTAINER, CONTAINER AND METHOD FOR INSTALLING THE MIXING DEVICE IN A CONTAINER - A mixing device for an application in a container including a rotatable shaft; a drive device through which the rotatable shaft is drivable; and at least one mixing element arranged at the shaft, wherein the mixing element is rotatable together with the shaft, wherein the shaft includes a support device connected with the shaft, wherein the support device is arranged in an end portion of the shaft that is oriented away from the drive device, wherein the shaft is rotatable about its longitudinal axis relative to the support device, wherein the mixing device is supportable through the support device in a receiving device connected with the container, wherein the support device is moveable by a straight bearing or a rolling element bearing along a guide device into the receiving device, wherein the straight bearing includes at least one sliding surface that is moveable on a corresponding sliding surface. | 04-02-2015 |
20150314613 | AUTOMATED DEVICES, SYSTEMS, AND METHODS FOR THE FABRICATION OF TISSUE - Described herein are improvements to bioprinting technology that facilitate automation of tissue and organ fabrication processes. | 11-05-2015 |
20150329818 | Procurement Stand For Stem Cell Collection And Transport - A stand for the collection of stem cells from a placenta. The stand includes a base with an upper surface adapted to hold a placenta during stem cell collection, a back support, and an upper platform with a surface suspended above the base and adapted to hold the placenta during collection of stem cells. The stand is preferably composed of cardboard or a similar material and is foldable between a folded configuration and an assembled configuration. The back support is hinged to the base and can move between a position parallel to the base in the folded configuration and a position perpendicular to the base in the assembled configuration. | 11-19-2015 |
20150344834 | System, Method and Devices for Tissue-Based Diagnosis - Described are devices, methods and systems for applying energy and/or a liquefaction promoting medium to a tissue of interest to generate a liquefied tissue sample. The liquefied tissue sample may be removed from the tissue of interest. Also described are compositions of the liquefaction promoting medium that may facilitate liquefaction, preserve liquefied tissue constituents, and/or enable delivery of molecules into tissues. The sample may include tissue constituents to provide for rapid tissue sampling, tissue decontamination as well as detection of analytes. One or more drugs may be delivered through or into the tissue of interest. Determination of tissue composition in the liquefied tissue sample can be used, e.g., for diagnosis or prognosis of diseases, evaluating bioavailability of therapeutic agents in different tissues following administration, forensic detection of drugs-of-abuse, evaluating changes in the tissue microenvironment following exposure to a harmful agent, and the like. | 12-03-2015 |
20150359949 | Polyp Filter - A polyp filter is non-integral to a corresponding suction-line dedicated polyp track. By one approach this polyp filter has a threaded portion that threadably engages at least one of a suction-line dedicated polyp trap housing and lid. That threaded portion can comprise, for example, a threaded hole that is formed axially through the polyp filter. By one approach that threaded portion threadably engages a threaded tube as comprises a part of the lid. | 12-17-2015 |
20160024456 | Method and Apparatus for Gender Selection Based on PH - The present invention includes methods and apparatus to separate X or Y-chromosome bearing sperm cells in a population by first placing the population of sperm cells at physiological pH environment, and simultaneously contacting the population of sperm cells with one or more additional sub-environments with different pH values. The exposure allows mobile sperm cells bearing X or Y-chromosome to migrate to the different pH sub-environments, wherein each cell only exposed or come in contact with one pH sub-environment. Finally, the collecting X or Y-chromosome enriched population of sperm cells is performed. | 01-28-2016 |
20160097701 | TISSUE PROCESSING AND SLIDE STAINING APPARATUS WITH ROBOTIC ARM HAVING THREE DEGREES OF FREEDOM - The embodiments herein provide a tissue processing and slide staining equipment with a three degree freedom robotic arm that simultaneously performs tissue processing and slide staining for several sample baskets with similar or different processing methods. The equipment comprises two dimensional array of cylindrical containers with lids to hold sample baskets in position. The arm moves freely in X, Y, and Z axis using rails and stepper motors. The arm is not connected to lids and baskets and accepts new baskets of samples while processing previous samples. The arm grips the basket containing samples and leaves the basket in proximity to the reagent. The equipment comprises a user friendly interface having appropriate callouts to guide a user during process programming/running. The interface allows the user to freely design and modify each step of the processing method. The equipment comprises an intelligent rule database that automatically handles human-induced errors. | 04-07-2016 |
20160115469 | DEVICE FOR ELECTRICALLY DISINTEGRATING CELL CLUSTERS | 04-28-2016 |
20160130548 | COMPOSITE SENSOR ASSEMBLIES FOR SINGLE USE BIOREACTORS - A composite sensor assembly for monitoring bio-processes which is suitable for use with a polymeric bioprocess vessel or with downstream equipment, and comprises: | 05-12-2016 |
20160138098 | CONTAINER ACCOMMODATION BODY - A container accommodation body includes: a package; a container that is accommodated and sealed in the package while accommodating and sealing a reagent for performing a nucleic acid amplification reaction; and a drying agent that is accommodated and sealed in the package. | 05-19-2016 |
20160160317 | METHOD AND SYSTEM FOR REMOVING SILICA FROM AN ORE - The present disclosure provides a beneficiation arrangement for removing silica from an ore. The beneficiation arrangement includes one or more scrubbing arrangements to scrub the ore with a stream of a washing fluid and recover the silica, one or more screening arrangements to perform screening of the scrubbed ore to extract a first quantity of recovered ore and an underflow, and one or more separating arrangements to enable separation of at least silica particles from the underflow. The one or more separating arrangements include one or more cyclonic separating arrangements to cyclonically separate the silica particles into one or more ranges of sizes of the silica particles from the underflow, and one or more filter press arrangements to perform pressure filtration on a recovered ore extract mix of the one or more recovered ore extract mix. | 06-09-2016 |
20160169777 | IN SITU HEAT INDUCED ANTIGEN RECOVERY AND STAINING APPARATUS AND METHOD | 06-16-2016 |
20160186118 | DEVICE FOR PUTTING MATERIAL INTO CELL - The present invention relates to a device for putting material into a cell to be modified. More particularly, the present invention is directed to a device formed within one solid material and comprises, a first passage on which the cell passes; a second passage on which the material passes and connected to the first passage at a position randomly selected between both ends of the first passage; and an apparatus which applies pressure difference or electric potential difference on the first passage and the second passage, and is also directed to a process for making the device. | 06-30-2016 |