Class / Patent application number | Description | Number of patent applications / Date published |
435306100 | Involving lysis of a microorganism by means other than comminution | 24 |
20090081772 | Method and device for collecting cellular material from cells isolated on a filter - Method for collecting cellular material of particular cells present in a liquid includes: | 03-26-2009 |
20090246866 | READY-TO-USE WHOLE BLOOD COLLECTION VESSEL - The present invention relates to a sampling tube for collecting and processing a whole blood sample. The sampling tube contains a reagent for differential hemolysis of whole blood, wherein said reagent for differential hemolysis comprises a chemical for differential hemolysis and an anti-coagulant, and wherein said sampling tube is a ready-to-use and single-use sampling tube. It also relates to the use of said sampling tube in the processing of a whole blood sample for liquid chromatography and also to the use of a blood sample processed in such sampling tube in a liquid chromatography-based analysis. | 10-01-2009 |
20110070638 | APPARATUS AND METHOD FOR PREPARATIVE SCALE PURIFICATION OF NUCLEIC ACIDS - Apparatus and methods are described for pharmaceutical grade manufacture extrachromosomal nucleic acids from cell lysates using flotation to separate and eliminate undesired insoluble cellular debris including chromosomal DNA from the lysates. A gas is introduced to controllably generate bubbles that reduce the density of the cell debris and create a buoyant flocculent phase that can be readily separated from, and thus provide, a substantially clarified fluid lysate phase that is enriched in extrachromosomal DNA but substantially depleted of cellular proteins and chromosomal DNA. | 03-24-2011 |
20110070639 | METHOD OF DESIGNING HYDRODYNAMIC CAVITATION REACTORS FOR PROCESS INTENSIFICATION - The present invention describes an apparatus of Hydrodynamic cavitation, to be used as reactors to achieve tangible effect by producing tailored active cavities either transient or steady or both, in aqueous and non-aqueous media for intensification of the physical and chemical processes in homogenous and heterogeneous systems. An apparatus comprises of a cavity generator, cavity diverter and turbulence manipulator wherein the cavity generator/cavity diverter is a flow modulator of various shapes and sizes. A regime map of cavitation and a method to generate it, is presented to achieve the desired type of cavitation, required for specific targeted process intensification and then reactors are designed to achieve the predetermined process intensification. Regime map relates the maximum fluid velocity in cavity generator with the cavitation number, active and specific type of cavity fraction for several geometric designs of apparatus. | 03-24-2011 |
20110124098 | Modular Microfluidic System for Biological Sample Preparation - A reconfigurable modular microfluidic system for preparation of a biological sample including a series of reconfigurable modules for automated sample preparation adapted to selectively include a) a microfluidic acoustic focusing filter module, b) a dielectrophoresis bacteria filter module, c) a dielectrophoresis virus filter module, d) an isotachophoresis nucleic acid filter module, e) a lyses module, and f) an isotachophoresis-based nucleic acid filter. | 05-26-2011 |
20110177588 | METHOD AND APPARATUS FOR DISRUPTING CELLS AND PURIFYING NUCLEIC ACID USING A SINGLE CHIP - Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited. | 07-21-2011 |
20110212517 | Method and device for preparing a sample of biological origin in order to determine at least one constituent contained therein - The invention relates to a method of preparing an original sample ( | 09-01-2011 |
20130137172 | HIGH DENSITY SELF-CONTAINED BIOLOGICAL ANALYSIS - Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include high density nucleic acid amplification and detection and immuno-PCR. | 05-30-2013 |
20130217113 | SYSTEM FOR AND METHODS OF PROMOTING CELL LYSIS IN DROPLET ACTUATORS - The invention relates to a droplet actuator for conducting droplet operations. The actuator includes a bottom substrate and a top substrate separated from the bottom substrate to form a gap. An arrangement of droplet operations electrodes may be located on a surface of the bottom substrate and/or top substrate. Optionally, a sample reservoir may hold a quantity of a sample fluid containing cells. A disruption device which can take various forms is used to lyse the cells in the sample or in a sample droplet to thereby conduct operations on samples having lysed cells therein. | 08-22-2013 |
20130323831 | INTEGRATED VERSATILE KIT FOR ISOLATING COMPONENTS IN BIOLOGICAL SAMPLES - The instant invention provides an intergrated kit with solutions and instruments to isolate various biological samples from biological samples. The kit enables an user to preserve specimens and isolate biomolecules with features and benefits of high quality, easy, fast, no toxicity, safe to user and environment, low demanding, cost-effective, reducing waste, saving nature resources and protecting environment, and leads to a low-carbon and Green economy in preparation of specimens. The integrated kit enables the user to extract biomolecules including DNA/ccfDNA, Large RNA/mRNA/ccfRNA, Small RNA/miRNA/ccfmiRNA, Protein, Lipid, Carbohydrates, and Metabolite using one seamless procedure. | 12-05-2013 |
20140080207 | ULTRASOUND AND ACOUSTOPHORESIS FOR COLLECTION AND PROCESSING OF OLEAGINOUS MICROORGANISMS - Microorganisms such as microalgae are collected and separated from a host medium such as water. Cellular walls and membranes of the microorganisms are then ruptured to release their lipids using a lipid extraction unit. Thereafter, the lipids from the host medium are collected and separated using a lipid collection and separation unit. Related apparatus, systems, techniques and articles are also described. | 03-20-2014 |
20140154797 | Lysis/Resealing Process and Device for Incorporating an Active Ingredient, in Particular Asparaginase or Inositol Hexaphosphate, in Erythrocytes - A lysis/resealing process for preparing erythrocytes containing active ingredient is provided comprising placing a globular concentrate in suspension in an isotonic solution having a haematocrit level which is equal to or greater than 65%, with refrigeration at 1 to 8° C.; measuring the osmotic fragility based on a sample of erythrocytes from that same globular concentrate, preferably on a sample of the suspension; lysis and internalisation procedure of the active ingredient, inside the same chamber, at a temperature maintained at 1 to 8° C., comprising allowing the erythrocyte suspension having a haematocrit level equal to or greater than 65% and a hypotonic lysis solution which is refrigerated at 1 to 8° C., to circulate in a dialysis cartridge; the lysis parameters being adjusted in accordance with the osmotic fragility previously measured; and resealing in a second chamber at a temperature of from 30 to 40° C. by means of a hypertonic solution. | 06-05-2014 |
20140178982 | REAGENT SUPPLY DEVICE FOR ANALYSIS OF NUCLEIC ACID - A reagent supply device includes a plurality of chambers which are separated from each other such that different reagents may be injected, the plurality of chambers having bottom surfaces made of a membrane, the membrane comprising a destruction pattern which is formed at a position corresponding to each of the plurality of chambers and breachable by an external impact to discharge the injected reagent from the chamber. | 06-26-2014 |
20140349386 | ULTRA-HIGH-SPEED NUCLEIC ACID EXTRACTING APPARATUS AND NUCLEIC ACID EXTRACTING METHOD USING SAME - A nucleic acid extraction device, which can realize automation, ultra-miniaturization and super-high speed in the nucleic acid extraction reaction, has no limitation to the type of biological specimens that can be used, such as sputum, blood, cells, urine, saliva, tissues, etc., minimize the used amount of the sample solution, and also maintain and/or improve the nucleic acid extraction efficiency with reliability. | 11-27-2014 |
20140356941 | CARTRIDGE FOR PREPARING A SAMPLE FLUID CONTAINING CELLS FOR ANALYSIS - A cartridge configured for use in a blood analyzer is provided. The cartridge may include a substantially rigid frame, a flow path within the rigid frame, at least one opening in the substantially rigid frame configured to align and stabilize a capillary tube, and a seal within the flow path. The seal may be configured to temporarily obstruct flow through at least a portion of the flow path. The seal may also be configured to open in response to a force exerted via a capillary tube inserted into the at least one opening. | 12-04-2014 |
20150024480 | SYSTEM, APPARATUS AND METHOD FOR MATERIAL PREPARATION AND/OR HANDLING - Oscillating angularly rotating a container containing a material may cause the material to be separate. Denser or heavier material may unexpectedly tend to collected relatively close to the axis of rotation, while less dense or light material may tend to collect relatively away from the axis of rotation. Oscillation along an arcuate path provides high lysing efficiency. Alternatively, a micromotor may drive an impeller removably received in a container. Lysing may be implemented in batch mode, flow-through stop or semi-batch mode, or flow-through continuous mode. Lysing particulate material may exceed material to be lysed or lysed material and/or air may be essentially eliminated from a chamber to increase lysing efficiency. | 01-22-2015 |
20150024481 | SAMPLE PREPARATION DEVICES AND SYSTEMS - Devices and system for preparing samples are described. Such devices can comprise fluidic chambers, reservoirs, and movable structures for controlling the movement of samples. The device can also comprise functional elements for performing specific operations. | 01-22-2015 |
20150031125 | MICRO-DEVICE AND METHODS FOR DISRUPTING CELLS - A micro-device for disrupting cells includes a first chamber in which the cells are disrupted, a second chamber which is pressurized and depressurized, a flexible membrane which separates the first chamber and the second chamber and is vibrated by pressuring and depressurizing the second chamber, and a micro-unit confined in the first chamber, where the micro-unit disrupts the cells in the first chamber | 01-29-2015 |
20150132841 | VESSEL HOLDER AND CAP ASSEMBLY - A vessel holder assembly is provided. A vessel holder, for receiving one or more vessels, includes a first set of attachment features. A cap assembly, for covering the vessel(s) includes a second set of attachment features. Respective attachment features of the vessel holder and the cap assembly may be complementary, such that engagement between complementary attachment members results in an attachment between the cap assembly and the vessel holder. Such an attachment may be manually removable, or may be locked so as to require a tool, or other suitable method, for detaching the cap assembly from the vessel holder. | 05-14-2015 |
20150352545 | APPARATUS AND METHOD FOR CELL DISRUPTION - An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses. | 12-10-2015 |
20160030939 | SAMPLE PREPARATION PAPER CARTRIDGE - A sample preparation cartridge is described having a lower portion having four integrally-formed leaf springs, and a raised lower base part which is slightly below the level of the leaf springs. An upper portion of the cartridge engages with the lower portion, via four paired pins and holes formed in the lower and upper portions. The upper portion includes three well-shaped openings formed in the central region of the upper portion. Between the upper and lower portions is placed a sheet of sample preparation paper, resting on the leaf springs, which raise the lower face of the paper away from the raised lower base part, leaving a small air gap between the base and the paper. The upper face of the paper is in contact with the well-shaped openings of the upper portion. The user loads a liquid sample into the openings, and the paper wicks the liquid sample away from the loading location, leaving cellular debris in the place of application. Applying pressure to the upper portion of the cartridge urges the paper into contact with the raised base portion, so providing a firm base which allows the user to remove sections of the paper, using for example a biopsy needle, for further processing. | 02-04-2016 |
20160046927 | METHODS AND SYSTEMS FOR DIFFERENTIAL EXTRACTION - Methods are provided for differential extraction of DNA from at least two different cell types. Systems for carrying out the differential extraction methods are also provided. A kit is also provided for differential extraction of DNA from at least two different cell types using a multi-compartment container. | 02-18-2016 |
20160108393 | Rapid Nucleic Acid Extraction Method and Apparatus - A syringe device having a plunger beneath which is disposed a rupturable capsule containing a nucleic acid wash buffer and a rupturing mechanism for rupturing the capsule, wherein the capsule, syringe, plunger, and rupturing mechanism are so configured as to first apply pneumatic pressure to force a nucleic acid solution through a membrane and to thereafter rupture the capsule and force a first volume of the wash buffer through the membrane. | 04-21-2016 |
20160186126 | ACOUSTIC ENERGY TREATMENT USING PHASE CHANGE COUPLANT - Method and apparatus for transmitting acoustic energy to a sample for treatment by employing a phase change couplant at least adjacent a sample vessel. The phase change couplant may be solid before and after treatment, but liquid during treatment to improve acoustic energy transmission charactersitics. | 06-30-2016 |