| Entries |
| Document | Title | Date |
|---|
| 20080199958 | Suspension Culture Vessels - A cell culture vessel comprising a housing chamber which has an inverted frusto-conical bottom having a vertical axis. The culture vessel further comprises an upper and lower section which are concentric. The vessel requires little or no shear-force and less stress by shaking or impellor action. | 08-21-2008 |
| 20080220524 | Three dimensional gum matrices for cell culture, manufacturing methods and methods of use - This invention relates three dimensional porous cell culture matrices or scaffolds which have directional porous structure. More particularly, this invention relates to three dimensional porous cell culture matrices or scaffolds for cell culture which are derived from or contain gums including naturally occurring gums, plant gums, galactomannan gums or derivatives thereof. The invention also relates to methods of making the matrices, articles of manufacture (e.g., cell culture vessels and labware) having such matrices or scaffolds, methods of applying these materials to cell culture surfaces, and methods of using cell culture vessels having these three dimensional porous cell culture matrices or scaffolds. | 09-11-2008 |
| 20080241926 | CELL ADHESION ON SURFACES OF VARYING TOPOGRAPHIES - Micro-topography of a surface influences cell adhesion and proliferation. To improve adhesion, polyelectrolyte multilayers (PEMs) are built on patterned support layers to increase surface wettability, thereby improving attachment and spreading of the cells. Physical parameters, such as pattern size and pitch, in part, regulate cell adhesion and proliferation. Varying the surface topography provides a method to influence cell attachment and proliferation for tissue engineering applications. | 10-02-2008 |
| 20080261306 | Method for Creating Perfusable Microvessel Systems - A method for creating networks of perfusable microvessels in vitro. A mandrel is drawn through a matrix to form a channel through the matrix. Cells are injected into the channel. The matrix is incubated to allow the cells to attach inside the channel. The channel is perfused to remove unattached cells to create a parent vessel, where the parent vessel includes a perfusable hollow channel lined with cells in the matrix. The parent vessel is induced to create sprouts into the surrounding matrix gel so as to form a microvessel network. The microvessel network is subjected to luminal perfusion through the parent vessel. | 10-23-2008 |
| 20080274545 | Bioreactor Chamber Apparatus, and Method and System for Fabricating and Mechanically Stimulating Natural and Engineered Tissues - A bioreactor chamber assembly including a bioreactor chamber and an outer chamber assembly is provided. In one design, the bioreactor chamber includes an upper grip assembly having a plurality of struts extending downward terminating in upper grips, each strut terminating in an upper grip, and a lower grip assembly containing one or more sample compartments and a lower grip. A sample can be formed in situ via injection into a sleeve contained within the sample compartment, where the sleeve encloses the upper and lower grips and enables a construct to form attached to these grips. In a second design, a split mold is received in the bioreactor chamber, the split mold having a cavity for receiving the upper and lower grips. Tissue explants and engineered constructs can be held within these grips. Medium level in the sample compartment is controlled, and different lengths of samples can be accommodated in the sample compartment. The height of the upper grip is adjustable by raising or lowering an extension rod that passes through a dynamic seal to the environment outside the chamber. The bioreactor chamber sample compartment includes a window to permit visualization of the sample as well as light-mediated transformation of biomaterials within the sample compartment. The samples held by grips within these chambers can be subject to uniaxial mechanical stimulation. Medium is perfused around the samples, and medium may be sampled via access ports. | 11-06-2008 |
| 20080293139 | CELL CULTURE SUPPORT FOR FORMING STRING-SHAPED CARDIOMYOCYTE AGGREGATES - This invention is intended to provide cell aggregates that can reproduce functions of the myocardium, such as the function of beating, and thus are available for myocardial regenerative therapy, and to provide a cell culture support for producing the same. | 11-27-2008 |
| 20090004737 | Microfluidic Devices and Methods for Fabricating the Same - A microfluidic device includes, in one embodiment, a first silk film coupled to a second silk film with at least one microchannel therebetween. | 01-01-2009 |
| 20090011507 | Scaffolds for Use in Tissue Engineering to Culture Cells - In the field of tissue engineering there is a need for a scaffold which is suited to low volume manufacture, and which allows the culture of complex cell structures. A scaffold ( | 01-08-2009 |
| 20090017540 | METHOD OF CULTURING CELLS UNDER REGULATION IN THE EXTENSION DIRECTION - [Problems]Relating to a bioassay method with the use of cultured cells and cell culture for a therapeutic purpose in the case of determining the efficacy of a drug or the like or examining the toxicity thereof, it is intended to provide a method of culturing cells under regulation in the extension direction and a cell culture plate. | 01-15-2009 |
| 20090087907 | Compositions and Methods for Growth of Pluripotent Cells - A method of propagating embryonic stem (ES) cells in an undifferentiated state, while maintaining both the pluripotency and the cells normal genotype is disclosed. The method comprises using recombinantly produced protein domains to attach human embryonic stem cells to the surface of a bioreactor. The ES cells are supplied with nutrients while they held in place by the recombinantly produced protein domains which may be chosen from Laminin G domain, Fibronectin domain 2, Fibronectin domain 3, Nidogen G2 domain, Nidogen G3 domain, Vitronectin somatomedin B domain, and Vitronectin somatomedin C terminal domain. Useful molecules are characterized by a high binding affinity for hES cells and a molecular weight of about 50 kDa±20%. | 04-02-2009 |
| 20090104699 | Container System for Tissue Stabilization for Molecular and Histopathology Diagnostics - A system for storing a biological sample for transfer between two different environments is provided. The system includes a first container defining a first container interior, and a second container defining a second container interior. A first closure is provided for enclosing the open end of the first container, with the first closure adapted to receive a sample holder. A second closure is also provided for enclosing the open end of the second container. The first container is adapted to removably receive the sample holder therein when the first closure encloses the open end of the first container, and the second container is adapted to subsequently receive the same sample holder therein when the first closure encloses the open end of the second container. | 04-23-2009 |
| 20090111180 | BIOREACTOR SYSTEM FOR THREE-DIMENSIONAL TISSUE STIMULATOR - A bioreactor system for growing and conditioning tissue for research and implantation in a human or animal body is disclosed which includes one or more tissue growth chambers for growing and conditioning tissue, each chamber being defined by a housing and providing a fluid culture media cavity which can act as a reservoir. A construct for growing three-dimensional tissues is housed in each tissue growth chamber. Each chamber is connected to a source of pressurized air for applying a controlled pressure to the chamber media cavity. The tissue growth chambers can be mounted on an agitation device such as a shaker system which enhances mass transport within the chamber media cavity. A control system is provided to control the pressure and temperature of the pressurized gas delivered to the chamber media cavity and subsequently to the tissue construct. | 04-30-2009 |
| 20090137042 | PROCESSES AND SYSTEMS FOR HYDRATING AND SEEDING MEDICAL IMPLANTS WITH BIOLOGICAL COMPONENTS - Methods and systems for providing an improved apparatus and packaging system to more expeditiously hydrate or reconstitute medical grafts and to effectively and uniformly seed the medical grafts with biological components and cultured cells. The systems generally comprise a container comprising entry port, at least one substrate cavity, and top, side and bottom walls defining an inner surface. The entry port is configured to receive the biological solution. The cavity is in communication with the entry port and includes the porous substrate maintained under negative pressure. The container volume is substantially the same as a volume of the porous substrate. The side and bottom walls are configured to promote a laminar flow of the biological solution received through the entry port. | 05-28-2009 |
| 20090142836 | BIOENGINEERED TISSUE CONSTRUCTS AND METHODS FOR PRODUCTION AND USE - Bioengineered constructs are formed from cultured cells induced to synthesize and secrete endogenously produced extracellular matrix components without the requirement of exogenous matrix components or network support or scaffold members. The bioengineered constructs of the invention can be treated in various ways such that the cells of the bioengineered constructs can be devitalized and/or removed without compromising the structural integrity of the constructs. Moreover, the bioengineered constructs of the invention can be used in conjunction with biocompatible/bioremodelable solutions that allow for various geometric configurations of the constructs. | 06-04-2009 |
| 20090186412 | POROUS CELL SCAFFOLD AND PRODUCTION METHOD THEREOF - For easily seeding cells in its scaffold, a porous cell scaffold is produced by steps of filling a guiding solution with kinematic viscosity being 50 to 450% of that of a culture medium, in a whole continuous small hole structure having hole diameters of 5 to 3200 μm and an average hole diameter of 50 to 1500 μm, of a sheet-shaped or block-shaped scaffold having a thickness of 2 mm or more, supplying thereafter a culture medium with cells being suspended to an upper side of the scaffold, sucking the guiding solution from a lower side of the scaffold by low suction force, and entering thereby the culture medium with cells being suspended into the whole small hole structure, where a water absorber such as a filter paper is preferably used for sucking the guiding solution by low suction force. | 07-23-2009 |
| 20090191631 | 3-D petri-dish for the culture and studies of cells - A three-dimensional (3-D) culture “Petri-dish” for research in regenerative medicine, biotechnology and clinical translation is described. This 3-D perfusion culture dish is to advance in vitro culture tools from static 2-D to dynamic 3-D perfusion culture. Interwoven hollow fiber capillary membranes divide the “Petri-dish” culture space into a controllable 3-D pattern of different compartments, serving the functions of the organ's larger vasculature. These physically active scaffolds, which can be suitable for cell adhesion or cell aggregate immobilization, offer a supply of cells with high-performance mass exchange including gas supply and under perfusion conditions. In contrast to static and discontinuous medium supply, a dynamic culture can be achieved with continuous or alternating medium supply and integral oxygenation. They provide a more physiologic supply in the cell macro environment, including homeostasis of oxygen, pH, nutrition, soluble factors, and gradients of metabolites for the cells. Also, medium perfusion can be achieved. Consequently the invention was made for cultures at tissue density, especially stem cells and support cells, which strive to create their own stem cell niche. | 07-30-2009 |
| 20090191632 | SWELLABLE (METH)ACRYLATE SURFACES FOR CULTURING CELLS IN CHEMICALLY DEFINED MEDIA - Synthetic surfaces capable of supporting culture of undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a peptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes hydroxyethyl methacrylate, 2-carboxyehylacrylate, and tetra(ethylene glycol) dimethacrylate. The conjugated peptide may include an amino acid sequence of Xaa | 07-30-2009 |
| 20090209035 | CELL OR TISSUE CULTIVATION APPARATUS AND METHOD OF CULTIVATION - A cultivation apparatus and a cultivation method of a culture such as a cell or tissue. Provided are an incubator unit for accommodating a culture and a lever that penetrates through the incubator unit and can move in circular arc around a fulcrum as a center, the fulcrum being set at a wall of the incubator unit or in the vicinity thereof, then displacement is imparted to the culture by operating the lever. Bending force can be acted on the culture (cell construct) such as a cell or tissue, so that without any increase or decrease of a culture fluid in the incubator unit, namely, without any increase or decrease of pressure to the culture fluid, displacement required for cultivation can be imparted to the culture in the incubator unit. By curving, continuous compression and extension are generated in a direction of thickness from a concave portion to a convex portion of the curving. | 08-20-2009 |
| 20090215177 | METHODS, SURFACE MODIFIED PLATES AND COMPOSITIONS FOR CELL ATTACHMENT, CULTIVATION AND DETACHMENT - The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity. | 08-27-2009 |
| 20090221076 | METHOD OF FABRICATING SHEET FOR CARTILAGE TISSUE REGENERATION - An objective of the present invention is to provide a production method of a sheet for regenerating a cartilage tissue, which uses a conventional sheet-shaped porous body comprising a biological absorbency synthetic high polymer, such as polylactic acid, polyglycolic acid and a copolymer of lactic acid and glycolic acid. The sheet for regenerating the cartilage tissue can differentiate chondrocytes or stem cells without culturing the cells by pressurizing such as a pressurizing culture like the conventional method, and can accumulate the cells in a supporting carrier with high efficiency. The sheet for regenerating the cartilage tissue is produced by the steps of; seeding chondrocytes or stem cells differentiating to the chondrocytes on a sheet-shaped porous body comprising a biological absorbency synthetic high polymer; taking the porous body into a culture liquid; applying acceleration of 100 to 1000 G to the porous body for a predetermined time; and culturing the porous body without applying the acceleration. | 09-03-2009 |
| 20090233361 | Tissue bioreactor - A bioreactor comprising, two chambers in fluid communication, a tissue matrix held in place between, and separating, the two chambers, each side of the tissue matrix in fluid communication with one of the two chambers, each chamber comprising at least one port. | 09-17-2009 |
| 20090253204 | Continuous flow chamber device for separation, concentration, and/or purification of cells - The present invention relates to methods and apparatuses for cell separation. In particular, the invention relates to separation of a particular cell type from a mixture of different cell types based on the differential rolling property of the particular cell type on a substrate coated with molecules that exhibits adhesive property with the particular cell type. This technology is adaptable for use in implantable shunts and devices for cell trafficking or tumor neutralization. | 10-08-2009 |
| 20090291499 | BIOLOGICAL TISSUE-REINFORCING MATERIAL, METHOD OF PRODUCING THE SAME, USE OF THE SAME, AND METHOD OF CULTURING CELLS - A method of producing a biological tissue-reinforcing material, which is applicable to a site required to be reinforced and which enables to improve the working properties for transplantation, comprising the step S | 11-26-2009 |
| 20090298181 | METHOD OF CULTIVATING CELL OR TISSUE - A method for cultivating a culture of a cell, tissue, etc. There is provided a method of cultivating a culture including a cell or tissue (cell construct), imparting bending motion to the culture. By virtue of applying bending force to a culture of a cell, tissue, etc. (cell construct) to thereby curve the culture, continuous compression and extension in a direction of thickness from a concave portion toward a convex portion thereof are induced. The physical stimulation and deformation not attained by conventional pressurization, shear and tension, then can be loaded on the culture to thereby realize the culture appropriate for restoration of tissue at a region accompanied by bending. | 12-03-2009 |
| 20100035344 | SOLUTIONS FOR TISSUE ENGINEERING AND METHODS OF USE - The present invention provides for solutions and methods of preparing a decellularized tissue for recellularization. The solutions provide collagen conditioning to restore collagen triple helix structure, strengthening of the collagen structure of the tissue, and biologically preparing the decellularized tissue by placing it in an environment that promotes recellularization. Methods and solutions for recellularization of a decellularized tissue, in accordance with the present invention, are also provided. | 02-11-2010 |
| 20100041148 | PROCESS AND SUPPORT STRUCTURE FOR THE CULTIVATION OF LIVING CELLS - In a process for the cultivation of living cells, in which the cells are cultivated on a support structure ( | 02-18-2010 |
| 20100041149 | BIOREACTOR AND METHOD FOR GENERATING CARTILAGE TISSUE CONSTRUCTS - A bioreactor includes a housing and a support mechanism for suspending the housing above the bottom surface of a culture vessel. The housing includes a member having oppositely disposed first and second surfaces and an inner surface defining an opening. The opening extends between the first and second surfaces of the member. The bioreactor includes a gas and liquid permeable membrane having first and second surfaces attached to the second surface of the member. The first surface of the gas and liquid permeable membrane and the member define a culture space for growing or culturing cells. | 02-18-2010 |
| 20100041150 | EXPANSION OF DEFINITIVE ENDODERM CELLS - Disclosed herein are cell cultures comprising expanded definitive endoderm cells as well as methods for expanding definitive endoderm cells in culture. | 02-18-2010 |
| 20100087004 | PEPTIDE-PRESENTING SURFACES FOR LONG-TERM CULTURE OF PLURIPOTENT CELLS - The present invention relates to methods of growing and maintaining pluripotent cells on an insoluble substrate that presents a peptide that binds to glycosaminoglycans, such as heparin. Specifically, methods of growing and maintaining pluripotent cells on substrates having a chemically defined surface presenting at least one peptide having basic amino acid residues separated by one or two hydrophobic amino acid residues. | 04-08-2010 |
| 20100112699 | POROUS COMPOSITE BIOMATERIALS AND PRODUCTION METHOD OF THE SAME - The invention discloses a porous composite biomaterial comprising of poly(γ-glutamic acid)-g-chondroitin sulfate (γ-PGA-g-CS) copolymer and poly(ε-caprolactone). The composite biomaterial provides a three-dimensional microenvironment for using as a scaffold for tissue engineering and for supporting the attachment and proliferation of cells. The invention also discloses a method of producing a porous composite biomaterial. | 05-06-2010 |
| 20100124783 | Methods and Compositions for Cell Attachment and Cultivation on Planar Substrates - The present invention is directed to methods for the growth, expansion and differentiation of pluripotent stem cells on planar substrates lacking an adlayer and a feeder cell layer. | 05-20-2010 |
| 20100136691 | PROCESS FOR THE PRODUCTION OF A STRUCTURE COMPRISING CRYSTALLINE CELLULOSE - In a process for the production of a structure comprising crystalline cellulose ( | 06-03-2010 |
| 20100151575 | Method of Making Conditioned Media from Kidney Derived Cells - Methods of making conditioned media by culturing cells on nonwoven substrates are disclosed. More specifically, methods of making conditioned media by culturing mammalian kidney-derived cells on nonwoven substrates are disclosed. | 06-17-2010 |
| 20100167401 | STACKED, PATTERNED BIOMATERIALS AND/OR TISSUE ENGINEERING SCAFFOLDS - Stacked, lamellar constructs comprised of, synthetic or natural, polymeric membrane structures which are brought together to form 3D scaffolds for biomaterial and guided tissue engineering applications have been developed. Each layer can have 2D or 3D nano and micro topographical features similar to or different than each other which can be arranged during the construction of each lamellae and their orientation can be adjusted during construction phase of the 3D structure. Such a construct was utilized in the development of an artificial cornea with human primary cells, in which patterned surface of the components of the lamellar structure mimics the oriented collagen structure inherent in natural cornea. Similar exploitation of the 3D patterned structure can be made for tissues where aligned ECM architecture is crucial, such as ligaments, bone, tendon, skin. | 07-01-2010 |
| 20100173415 | Carrier for cell cultivation - A carrier for cell cultivation having stress of 10-30 kPa at 10% load, preparing a material, which has physical property similar to cartilage tissue of vivo, by lyophilization using high concentrated collagen dispersion, solution or mixture thereof as a starting material, then performing insolubilizing treatment to regulate physical strength and absorption speed in a living body. | 07-08-2010 |
| 20100173416 | Method and Apparatus for Inoculating and Streaking a Medium in a Plate - An apparatus for inoculating and streaking a solid growth culture medium in a plate, the streaking using a streaking applicator having a line of resiliently and flexibly supported spaced apart contact surfaces, the apparatus including: (a) an inoculating and streaking station including: a plate work position having a notional action line fixed in two dimensions (x,y) in a predetermined position; and a plate rotation device for rotating a positioned plate to cause streaking; (b) a sensor capable of locating the surface of the medium in a positioned plate to thereby determine for that plate, prior to inoculation and streaking of that plate, the third dimension (z) of the action line; (c) an inoculating device capable of dispensing inoculum, along the action line, on the surface of the medium in the positioned plate; and (d) a streaking device capable of moving the streaking applicator such that its line of spaced apart contact surfaces contacts, along the action line, the surface of the medium in the positioned plate, prior to rotation of the positioned plate for streaking. | 07-08-2010 |
| 20100184220 | PREVASCULARIZED TISSUE TRANSPLANT CONSTRUCTS FOR THE RECONSTRUCTION OF A HUMAN OR ANIMAL ORGAN - The invention relates to a tissue transplant construct for the reconstruction of a human or animal organ. Here said tissue transplant construct is intended to comprise | 07-22-2010 |
| 20100184221 | METHOD FOR ISOLATION OF CELL, SERUM-FREE CULTURE MEDIUM FOR CELL, AND METHOD FOR CULTURE OF CELL - The present invention provides a method for isolating cells, in particular, stem cells, which method comprises the steps of: dissociating cells from a tissue of animal origin; seeding and incubating the cells in a culture vessel with no surface treatment; and selecting cells adhered to the vessel. | 07-22-2010 |
| 20100184222 | METHOD FOR PREPARING BIOLOGICAL TISSUE - This invention provides a method for preparing biological tissue in which the thickness (i.e., the number of cell layers) can be easily regulated, culture can be conducted within a shorter period of time than is possible with conventional techniques, and addition of unfavorable components is not necessary. The method of the invention comprises adding a cell-containing culture solution to a culture vessel having a non-cell-adhesive inner bottom surface, conducting cell culture while centrifugal force toward the inner bottom surface is applied to the cells in the vessel, forming tissue via intercellular adhesion, and detaching and collecting the resulting tissue from the inner bottom surface. | 07-22-2010 |
| 20100203638 | Method of Producing High-Density Cultured Tissue and High-Density Cultured Tissue - It is intended to provide a method comprising providing a mesh member and a liquid flow controlling member in a channel, in which a cell culture liquid containing an extracellular matrix component and animal cells of one or more types are cultured under circulation, so that the liquid flow-controlling member is located in contact with or close to the back face of the mesh member with respect to the liquid flow, and thus collecting the polymerized extracellular matrices and the animal cells at a high density on the surface of the mesh member. According to this method, an artificial tissue similar to a biological tissue, in which cells are collected at a high density, can be quickly constructed by a simple procedure. | 08-12-2010 |
| 20100216239 | ISOLATED NATURE-IDENTICAL COLLAGEN - The present invention concerns isolated collagen, the method for its production, and isolation of the collagen from collagen-containing tissues, as well as the use of the isolated collagen in a biomatrix as an in vitro test system, tissue replacement or organ replacement. | 08-26-2010 |
| 20100216240 | NON-INVASIVE AUTOMATED CELL PROLIFERATION APPARATUS - A cell proliferation apparatus for the automated culturing of cells, the proliferation apparatus including a bioreactor having contained therein a stimulus-responsive three dimensional (3D) cell scaffold, the stimulus-responsive three-dimensional (3D) cell scaffold being operable reversibly to change its surface properties between hydrophilic and hydrophobic states. The cell scaffold comprises a surface layer of thermo-responsive polymer. | 08-26-2010 |
| 20100216241 | FORMING CELL STRUCTURE WITH TRANSIENT LINKER IN CAGE - In a method of forming a cellular structure, cells and a transient linker are supplied to a volume partially enclosed by a cage. The linker facilitates initial attachment of adjacent cells to form a cell aggregate. The cage defines distributed openings that are sized to retain the cell aggregate. A fluid comprising a cell culture medium is supplied to the volume. The fluid is withdrawn from the volume through the openings. Aggregated cells retained in the volume are cultured to form a cell structure. A cell culturing device is provided which comprises a conduit and a cage in the conduit. A fluid flows in the conduit. The fluid comprises the cells, the transient linker and the cell culture medium. The cage retains aggregated cells formed in the fluid, and defines distributed openings that allow the fluid to flow through. | 08-26-2010 |
| 20100221834 | LIQUID CRYSTALLINE SUBSTRATES FOR CULTURING CELLS - The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e.g., reorganization) of extracellular matrix components by living cells. | 09-02-2010 |
| 20100227405 | METHODS OF USING MULTILAYERED CELL CULTURE APPARATUS - A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications. | 09-09-2010 |
| 20100233811 | CELL SEEDING MODULE - An apparatus and method is described for seeding cells on a sample or specimen. The cells may be selectively and locally seeded on an upper and lower surface of a planar sample or specimen or on either or both of an interior luminal surface and exterior surface of a hollow sample or specimen. The apparatus includes a chamber suitable for cell seeding, cell growth, and cell conditioning. | 09-16-2010 |
| 20100267142 | SCALABLE PACKED-BED CELL CULTURE DEVICE - A scalable packed-bed cell culture device includes a matrix vessel, a mixing vessel, a communicating means, a driving means and a controlling means. The matrix vessel includes porous matrixes packed therein. The mixing vessel includes a mixing means configured for mixing a culture medium. The communicating means is connected between the matrix vessel and the mixing vessel. The driving means is configured for driving the culture medium to flow between the matrix vessel and the mixing vessel. The controlling means configured for controlling the culture medium to submerge the porous matrixes at high level, and to emerge the porous matrixes at low level. An inoculation method and a culture method for scalable packed-bed cell culture device is also herein provided for eliminating the limitation of aeration or oxygenation during culture, alleviating the gradient effect, eliminating the channeling effect in conventional packed-bed bioreactors. | 10-21-2010 |
| 20100297767 | NOVEL CELL CULTURE AND METHODS OF PRODUCING AND COLLECTING CELL MASSES USING THE SAME - The inventors of the present invention have developed a novel cell culture method with a view to providing a process by which cell masses uniform in size and properties can be produced in large quantities and with convenience, as well as a method of recovering them. | 11-25-2010 |
| 20110020933 | METHOD AND DEVICE FOR RECEIVING BIOLOGICAL CELLS FROM A STEM CELL CULTURE - A method for receiving biological cells ( | 01-27-2011 |
| 20110033932 | SUBSTRATE FOR CELL ADHESION OR CULTURE AND METHOD FOR PRODUCING THE SAME - A substrate for cell adhesion or culture of the invention comprises: a base material; a cell adhesion layer arranged to cover a predetermined region on this base material; and a non-cell adhesion layer arranged on the base material to cover a region other than the predetermined region. An exposed surface of the cell adhesion layer is a cell adhesion surface. The non-cell adhesion layer has a light transmission characteristic, and an exposed surface of the non-cell adhesion layer is a non-cell adhesion surface. | 02-10-2011 |
| 20110033933 | METHOD APPLYING HEMODYNAMIC FORCING AND KLF2 TO INITIATE THE GROWTH AND DEVELOPMENT OF CARDIAC VALVES - A method for forming a cardiovascular structure in culture is provided. The method includes applying mechanical force to a cell population in culture such that a cardiovascular structure is formed. In some embodiments, the mechanical force is produced in culture medium by a pulsatile liquid flow with a retrograde component. The cell population can include stem cells or differentiated cells, or combinations of both. In particular embodiments, a cardiovascular valve is formed. Scaffolds for the support and growth of the cell population, and bioreactors including the scaffolds, are also provided. | 02-10-2011 |
| 20110059527 | Active Cell Culture Via Shape Memory - Substrates for cell culture and tissue engineering bioreactors consisting of polymers that change their shape over time under stimulation by temperature change, hydration, degradation, or other means. A method of controlling cell culture using a biodegradable shape memory polymer, wherein shape changes can transfer stresses, strains, or both to adherent or otherwise connected cells such that the mechanical stimulus impacts cell development and the resulting properties of tissues. | 03-10-2011 |
| 20110086427 | MICROFLUIDIC DEVICE FOR CELL CULTURE - A microfluidic cell culture apparatus includes a cell retention chamber and a perfusion channel. The cell retention chamber has a structured surface. The structured surface includes a major surface from which a plurality of projections extends into the chamber. The plurality of projections are arranged to suspend cells cultured in the chamber above the major surface. The first perfusion channel is configured to provide laminar flow of a fluid through the channel and forms a plurality of openings in communication with the cell retention chamber. The openings are configured to prevent cells from the retention chamber from entering the perfusion channel. | 04-14-2011 |
| 20110091972 | Fabricating Scaffolds and Other Cell-Growth Structures Using Microfluidics to Culture Biological Samples - Methods and apparatuses for using microfluidics to generate bubbles and using the generated bubbles to construct scaffolds and cell-holding structures for culturing biological samples or analytes. In one implementation, a scaffold for growing cells is provided to include a matrix of interconnected cavities formed from mixing a gas and a liquid containing a cross linkable material to produce a matrix of gas bubbles of substantially the same size and cross linking the cross linkable material to form a structure in which cells are grown. In another implementation, a scaffold apparatus for growing cells includes a ball of a cross linked material forming an exterior shell that encloses to form a hollow interior inside the ball and biological samples embedded in the external shell. | 04-21-2011 |
| 20110111504 | BIOREACTOR AND METHOD FOR CULTIVATING CELLS AND TISSUES - The invention relates to a bioreactor ( | 05-12-2011 |
| 20110129923 | CELL CULTURE METHODS AND DEVICES UTILIZING GAS PERMEABLE MATERIALS - Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency. | 06-02-2011 |
| 20110151563 | Collagen Materials, Films and Methods of Making Same - In general, the present invention is related to collagen compositions and thin films, and to methods of making and using the same. In some embodiments, the present invention is directed to “woven pattern” or “basket pattern” collagen compositions and thin films, and methods of making. | 06-23-2011 |
| 20110171732 | HIGHLY POROUS SOLID MATERIAL MADE OF BIODEGRADABLE POLYMER AND METHOD OF FABRICATING, PROCESSING, AND CELL-SEEDING THE SAME - [Problems] A purpose of the invention is to provide highly porous 3D scaffolds made of biodegradable polymer such as poly-lactic acid, which can be preferably used for cell culture. | 07-14-2011 |
| 20110201116 | TEST METHOD USING CELLS AND TEST KIT THEREFOR - The present invention provides a method for performing a biological test under conditions in which an artificially prepared cell pattern with initial position coordinates that can be determined is three-dimensionally cultured within a gelled matrix. The present invention relates to a biological test method that comprises testing a biological indicator with reference to at least one selected from the group consisting of cell proliferation, cell movement, and cell differentiation in a cell pattern substantially embedded in gel. The present invention also relates to a kit for the biological test method. | 08-18-2011 |
| 20110212524 | BIOMATERIAL AND PREPARATION METHOD THEREOF - A method for preparing a biomaterial comprising the steps of acellularizing the fish scale to remove cell components, decalcifying the fish scale; and cleaning the fish scale, wherein in the step of acellularizing, it further including stirring constantly the fish scale in a solution comprised of an octylphenoxypolyethoxyethanol, a tris-buffered salt and a protease inhibition; and rinsing in a Hanks' buffered saline and the fish scale keeps the naturally 3-dimension microstructure after the step of acellularizing the fish scale. | 09-01-2011 |
| 20110244571 | CELL GROWTH - Methods of preparing pre-engineered surfaces using various nanolithography techniques to generate, isolate, and multiply homogeneous cell populations. Surfaces can be treated by etching before exposure to biological systems like cells. Stem cell applications are described. | 10-06-2011 |
| 20110250688 | Three Dimensional Tissue Generation - Methods and materials for generating a three-dimensional tissue construct are provided. The method comprises (a) providing a tissue scaffold; (b) providing isolated smooth muscle cells; (c) suspending the isolated cells in hydrogel; (d) ejecting the cell/hydrogel suspension from a printer onto the tissue scaffold; and (e) incubating the cells on the scaffold in media at 37° C. | 10-13-2011 |
| 20110281358 | System For Seeding Cells Onto Three Dimensional Scaffolds - Systems are provided for convenient and sterile isolation, collection, and seeding of cells onto a scaffold or tissue graft. The systems may be closed. Methods for use of the disclosed systems for isolation, collection and seeding of cells and generation of tissue engineered vascular grafts are also provided. The systems may be supplied in kits for efficient and expeditious use. | 11-17-2011 |
| 20110287542 | CELL CULTURE METHODS AND DEVICES UTILIZING GAS PERMEABLE MATERIALS - Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency. | 11-24-2011 |
| 20110287543 | CELL CULTURE METHODS AND DEVICES UTILIZING GAS PERMEABLE MATERIALS - Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency. | 11-24-2011 |
| 20110306134 | RETENTION OF A STEM CELL PHENOTYPE - A method for promoting the retention of a stem cell phenotype in a population of stem cells. A biocompatible substrate is provided, e.g. in the form of cultureware. The substrate has an arrangement of topographical features arrayed in a pattern based on a notional symmetrical lattice in which the distance between nearest neighbour notional lattice points is between 10 nm and 10 μm, and wherein the topographical features are either located in register with the respective notional lattice points or are locally misordered such that the centre of each topographical feature is at most 10% of the distance between nearest neighbour notional lattice points from its respective notional lattice point. A population of stem cells is provided in contact with said arrangement of topographical features. Culturing the population of stem cells under conditions that allow the stem cells to proliferate allows the retention of the stem cell phenotype in the population of stem cells. | 12-15-2011 |
| 20110306135 | METHOD FOR PRODUCING TOOTH - A method for producing a tooth having a desired length in one direction includes the steps of: placing a first cell aggregate and a second cell aggregate in the inside of a support while bringing the first and the second cell aggregates into close contact with each other; and culturing the first and the second cell aggregates in the inside of the support, in which the first cell aggregate is composed of one of mesenchymal cells or epithelial cells and the second cell aggregate is composed of the other, and the size of the tooth is controlled by adjusting the length of contact between the first cell aggregate and the second cell aggregate in one direction. | 12-15-2011 |
| 20120077272 | CELLULAR SCAFFOLD - A cellular scaffold that is suitable for tissue regeneration, cell culture and in vitro assays. The invention relates to a layered cell scaffold that is seeded with mesenchymal and ectodermal cells. The layered cellular scaffold comprises an inoculum of mesenchymal cells and ectodermal cells positioned between two opposing scaffolds in a sandwich configuration. The layered cell scaffold provides a functional skin equivalent that is suitable for transplantation and in vitro cell-based assays. | 03-29-2012 |
| 20120100612 | CULTURE SUBSTRATE, CULTURE SHEET, AND CELL CULTURE METHOD - Disclosed is a culture sheet which enables technology in which three-dimensional tissues with uniform diameter are formed without applying chemicals to the surface of a culture substrate. A plurality of holes are formed on the culture sheet of the substrate, and nanopillars capable of controlling the adhesiveness or migration of a cell are formed on a culture surface that serves as the bottom surface of each of the holes. The culture surface of each of the holes having a structure in which a partition wall is provided, wherein, by forming the internal nanopillars in the vicinity of the center of each of the holes, the interaction of the disseminated cells can be limited to uniform the size of the three-dimensional structures of the cells to be formed. | 04-26-2012 |
| 20120107935 | Simplicity in assembling microstructures - Concept and examples are provided for simplicity in assembling micro-culture structures. A cell culture could be started prior to the formation of a microstructure. Cultured cells are utilized as glue and spacers to measure and assemble the microstructure automatically. | 05-03-2012 |
| 20120129257 | Apparatus For Culturing Anchorage Dependent Cells - The invention relates to an apparatus ( | 05-24-2012 |
| 20120149111 | Porous Polymer Scaffolds for Neural Tissue Engineering and Methods of Producing the Same - The present invention relates to scaffolds that can physically guide cells, e.g. neurons, while best matching the material properties of native tissue. The present invention also relates to methods of generating such scaffolds, and for the use of such scaffolds, e.g. in spinal cord and peripheral nerve injury repair. The methods of the present invention include a uniquely controlled freeze casting process to generate highly porous, linearly oriented scaffolds. The scaffolds of the present invention not only comprise a highly aligned porosity, but also contain secondary guidance structures in the form of ridges running parallel to the pores to create a series of microstructured and highly aligned channels. This hierarchy of structural guidance aligns and guides neurite outgrowth down the channels created by the ridges, and keep neurites from branching perpendicular to the inter-ridge grooves. | 06-14-2012 |
| 20120171768 | COLLAGEN-BASED MATRICES FOR VESSEL DENSITY AND SIZE REGULATION - Collagen based-matrices and methods of their use are described. More particularly, collagen-based matrices and methods for regulating vessel density and size within collagen-based matrices are described. | 07-05-2012 |
| 20120190113 | Macroporous Microcarrier Specific to Liver Cell, Preparation Method and Use Thereof - The present invention provides a macroporous microcarrier specific to hepatocytes using silk fibroin and galactosylated chitosan as main raw material, a preparation method thereof, and application for hepatocyte culture under the culture condition of microgravity rotation. The macroporous microcarrier s a sphere prepared from silk fibroin and galactosylated chitosan under the effect of crosslinker, wherein based on the total weight of the sphere, the content of silk fibroin is 50-80 wt % and the content of galactosylated chitosan is 15-40 wt %. The diameter of the microcarrier is 200-500 μm, and the aperture of the microcarrier is 40-80 μm. Compared with normal solid scaffold material, the microcarrier provided by the present invention has larger surface area/volume ratio and, a sinus gap structure extremely similar with in-vivo liver sinus structure, therefore it is more conducive to adhering of the hepatocytes on the scaffold material, contacting between cells, transporting oxygen and nutrient components and excreting metabolic products. | 07-26-2012 |
| 20120238021 | METHODS OF SYNTHESIZING THREE-DIMENSIONAL HETEROATOM-DOPED CARBON NANOTUBE MACRO MATERIALS AND COMPOSITIONS THEREOF - Methods for synthesizing macroscale 3D heteroatom-doped carbon nanotube materials (such as boron doped carbon nanotube materials) and compositions thereof. Macroscopic quantities of three-dimensionally networked heteroatom-doped carbon nanotube materials are directly grown using an aerosol-assisted chemical vapor deposition method. The porous heteroatom-doped carbon nanotube material is created by doping of heteroatoms (such as boron) in the nanotube lattice during growth, which influences the creation of elbow joints and branching of nanotubes leading to the three dimensional super-structure. The super-hydrophobic heteroatom-doped carbon nanotube sponge is strongly oleophilic and an soak up large quantities of organic solvents and oil. The trapped oil can be burnt off and the heteroatom-doped carbon nanotube material can be used repeatedly as an oil removal scaffold. Optionally, the heteroatom-doped carbon nanotubes in the heteroatom-doped carbon nanotube materials can be welded to form one or more macroscale 3D carbon nanotubes. | 09-20-2012 |
| 20120288937 | BIOREACTOR FOR CONTROLLING CELLULAR GROWTH - A system includes a first chamber configured to receive a hydrogel and a scaffold comprising a cell, wherein the hydrogel is in fluid communication with the scaffold, and wherein the hydrogel includes a plurality of unidirectional pores. The system also includes a second chamber configured to receive a first fluid and a second fluid, wherein the second chamber includes a wall that separates the first fluid from the second fluid. The system further includes a porous membrane configured to separate the first chamber from the second chamber. The wall is configured to move along the porous membrane as cellular extensions are projected into at least a portion of the plurality of unidirectional pores of the hydrogel. | 11-15-2012 |
| 20120288938 | SELF-ASSEMBLING CELL AGGREGATES AND METHODS OF MAKING ENGINEERED TISSUE USING THE SAME - A composition comprising a plurality of cell aggregates for use in producing engineered organotypic tissue by organ printing. A method of making a plurality of cell aggregates comprises centrifuging a cell suspension, extruding the resulting pellet through an orifice, and cutting the extruded pellet into pieces. Apparatus for making cell aggregates comprises an extrusion system and a cutting system. In a method of organ printing, a plurality of cell aggregates are embedded in a polymeric or gel matrix and allowed to fuse to form a desired three-dimensional tissue structure. An intermediate product comprises at least one layer of matrix and a plurality of cell aggregates embedded therein in a predetermined pattern. Modeling methods predict the structural evolution of fusing cell aggregates for combinations of cell type, matrix, and embedding patterns to enable selection of organ printing process parameters for use in producing an engineered tissue having a desired three-dimensional structure. | 11-15-2012 |
| 20120322154 | APPARATUS AND METHOD FOR MANUFACTURING CELL CULTURE SCAFFOLD - A cell culture scaffold manufacturing apparatus according to an exemplary embodiment of the present invention includes a solution storage portion in which a biopolymer solution is stored; a plotter that includes a plotter nozzle for ejecting a solution supplied from the solution storage portion; and a cylindrical collection portion that has a cylinder shape and is disposed at a lower portion of the plotter so that the solution ejected through the plotter nozzle of the plotter is collected. | 12-20-2012 |
| 20120322155 | Novel Biodegradable Elastomeric Scaffold for Tissue Engineering and Light Scattering Fingerprinting Methods for Testing the Same - The present invention is directed to a novel biocompatible polymer that may be used in tissue engineering. More specifically, the specification describes methods and compositions for making and using a citric acid copolymers. | 12-20-2012 |
| 20130029420 | METHOD OF CULTURING VASCULAR SMOOTH MUSCLE CELLS, CULTURE DEVICE AND MEDICAL MATERIAL OBTAINED BY THE CULTURE - There is provided a method for culturing vascular smooth muscle cells while maintaining their normal function, and a culture device and regenerative medical material for the same. The method takes advantage of vascular smooth muscle cell recognition of elastin as an extracellular matrix. The invention provides a method for culturing vascular smooth muscle cells on elastin, a culture device having elastin anchored on the cell-growing surface, a culture device wherein the cell-growing surface is composed of an elastin molded article, and medical materials obtained by culturing vascular smooth muscle cells using such culture devices. | 01-31-2013 |
| 20130045535 | NICHE SYSTEM FOR BIOLOGICAL CULTURING - A cell culturing system is provided. The cell culturing system includes a cell niche defined by a niche base and niche walls, at least one of which includes a fluid pathway formed therein. The niche wall material is selected capable of enabling diffusion into the niche of a fluid flowing between an inlet and an outlet of the fluid pathway. | 02-21-2013 |
| 20130109093 | Method of Producing Tooth, Set of Teeth, and Method of Producing Tissue | 05-02-2013 |
| 20130130383 | ULTRAHIGH SURFACE AREA SUPPORTS FOR NANOMATERIAL ATTACHMENT - The present invention is directed to a hierarchical structure characterized by ultrahigh surface area comprising: a solid substrate; an intermediate layer; and at least one plurality of nanoscale attachments that are strongly bonded to the intermediate layer. Also disclosed is a method of fabricating a hierarchical structure comprising: selecting and preparing a parent substrate, wherein the preparing may optionally include cleaning or activation; modifying the substrate surface to form an intermediate layer; attaching at least one plurality of nanoscale attachments, wherein the nanoscale attachments are selected from nanotubes, nanoparticles, or combinations thereof, onto the intermediate layer; optionally attaching a second plurality of nanoscale attachments, wherein the nanoscale attachments are selected from nanotubes, nanoparticles, or combinations thereof, onto the first plurality of nanoscale attachments and intermediate layer. | 05-23-2013 |
| 20130196438 | TISSUE ENGINEERED BLOOD VESSELS - Compositions and methods of using tissue engineered blood vessels to repair and regenerate blood vessels of patients with vascular disease are disclosed. | 08-01-2013 |
| 20130196439 | TISSUE ENGINEERED BLOOD VESSEL - Compositions and methods of using tissue engineered blood vessels to repair and regenerate blood vessels of patients with vascular disease are disclosed. | 08-01-2013 |
| 20130196440 | TISSUE ENGINEERED BLOOD VESSEL - Compositions and methods of using tissue engineered blood vessels to repair and regenerate blood vessels of patients with vascular disease are disclosed. | 08-01-2013 |
| 20130252337 | SUBSTRATES WITH MICROMETER AND NANOMETER SCALE STIFFNESS PATTERNS FOR USE IN CELL AND TISSUE CULTURING AND A METHOD FOR MAKING SAME - A substrate comprising a base, first regions having a first stiffness value and disposed above the base, second regions having a second stiffness value disposed above the base, wherein the first stiffness value is different from the second stiffness value, and wherein a portion of one or more of the first regions and a portion of one or more of the second regions is exposed on an upper surface of the substrate. | 09-26-2013 |
| 20130309770 | SCAFFOLDS AND OTHER CELL-GROWTH STRUCTURES USING MICROFLUIDICS TO CULTURE BIOLOGICAL SAMPLES - Methods and apparatuses for using microfluidics to generate bubbles and using the generated bubbles to construct scaffolds and cell-holding structures for culturing biological samples or analytes. In one implementation, a scaffold for growing cells is provided to include a matrix of interconnected cavities formed from mixing a gas and a liquid containing a cross linkable material to produce a matrix of gas bubbles of substantially the same size and cross linking the cross linkable material to form a structure in which cells are grown. In another implementation, a scaffold apparatus for growing cells includes a ball of a cross linked material forming an exterior shell that encloses to form a hollow interior inside the ball and biological samples embedded in the external shell. | 11-21-2013 |
| 20130323839 | Culture Substrate and Culture Sheet - Provided is a culture sheet which enables a technique for forming a three-dimensional tissue having uniform diameter without applying any chemical on the surface of a culture substrate. On the culture sheet ( | 12-05-2013 |
| 20130344600 | COMPOSITIONS COMPRISING NANOSTRUCTURES FOR CELL, TISSUE AND ARTIFICIAL ORGAN GROWTH, AND METHODS FOR MAKING AND USING SAME - The invention provides articles of manufacture comprising biocompatible nanostructures comprising nanotubes and nanopores for, e.g., organ, tissue and/or cell growth, e.g., for bone, kidney or liver growth, and uses thereof, e.g., for in vitro testing, in vivo implants, including their use in making and using artificial organs, and related therapeutics. The invention provides lock-in nanostructures comprising a plurality of nanopores or nanotubes, wherein the nanopore or nanotube entrance has a smaller diameter or size than the rest (the interior) of the nanopore or nanotube. The invention also provides dual structured biomaterial comprising micro- or macro-pores and nanopores. The invention provides biomaterials having a surface comprising a plurality of enlarged diameter nanopores and/or nanotubes. | 12-26-2013 |
| 20140024117 | Extracellular Matrix Films And Methods Of Making And Using Same - In one aspect, films that can serve as a model or mimic for the basal lamina are described herein. In some embodiments, a film described herein comprises a top surface and a bottom surface in facing opposition to the top surface, wherein the film is formed from Type I collagen and one or more additional extracellular matrix proteins. The additional extracellular matrix proteins, in some cases, comprise one or more of Type IV collagen, laminin, and fibronectin. Moreover, in some instances, the weight ratio of Type I collagen to the additional extracellular matrix proteins in the film is at least about 40:1. | 01-23-2014 |
| 20140080214 | CLEAVABLE CELLULOSIC SPONGE DEVELOPMENT FOR 3 DIMENSIONAL CELL CULTURE AND SPHEROIDS RETRIEVAL - A method of making a scaffold for 3 dimensional cell culture comprising the steps of conjugating a reducible disulfide bond onto a hydroxyl group at the side chain of a hydroxypropyl cellulose; forming a matrix of hydroxypropyl cellulose having the reducible disulfide bond conjugated onto the hydroxyl group such that a reducible disulfide bond exists adjacent to a double bond for crosslinking the matrix of hydroxypropyl cellulose. The scaffold and a system of using the scaffold for culturing 3 dimensional cell spheroids | 03-20-2014 |
| 20140113373 | THREE DIMENSIONAL SOY PROTEIN-CONTAINING SCAFFOLDS AND METHODS FOR THEIR USE AND PRODUCTION - Porous soy protein-based scaffolds and methods for making the scaffolds using 3D printing techniques are provided. Also provided are tissue growth scaffolds comprising the porous soy protein-based scaffolds and methods for growing tissue on the tissue growth scaffolds. The porous soy protein-containing scaffold comprises a plurality of layers configured in a vertical stack, each layer comprising a plurality of strands comprising denatured soy proteins. | 04-24-2014 |
| 20140120619 | SYSTEM AND METHOD FOR PRODUCING TISSUE-ENGINEERED MATERIAL - A system for producing a tissue-engineered material includes a hollow member and a mechanical stimulating unit. The hollow member is adapted to be implanted in a peritoneal cavity, and is to be positioned in the peritoneal cavity in a manner that a part of the hollow member contacts an inner wall surface of the peritoneal cavity for enabling formation of a biological tissue that encapsulates the hollow member. The mechanical stimulation unit is coupled to the hollow member and configured to provide a periodic mechanical stimulus to the biological tissue by periodically causing the hollow member to expand and contract. A method for producing the aforesaid tissue-engineered material is also disclosed. | 05-01-2014 |
| 20140127808 | POROUS CELL SCAFFOLD AND PRODUCTION METHOD THEREOF - For easily seeding cells in its scaffold, a porous cell scaffold is produced by steps of filling a guiding solution with kinematic viscosity being 50 to 450% of that of a culture medium, in a whole continuous small hole structure having hole diameters of 5 to 3200 μm and an average hole diameter of 50 to 1500 μm, of a sheet-shaped or block-shaped scaffold having a thickness of 2 mm or more, supplying thereafter a culture medium with cells being suspended to an upper side of the scaffold, sucking the guiding solution from a lower side of the scaffold by low suction force, and entering thereby the culture medium with cells being suspended into the whole small hole structure, where a water absorber such as a filter paper is preferably used for sucking the guiding solution by low suction force. | 05-08-2014 |
| 20140127809 | Perfusion Device and Method - A perfusion device and method is provided. In one embodiment, the device includes a container having a first internal chamber configured to hold the material; a port for introducing the liquid into the chamber; a vent for releasing gas and liquid from the chamber; and a means for sealing the vent to allow a vacuum to be drawn on the first chamber The material may be biomaterial, such as a bone graft material in any form. In one embodiment, the container is a syringe that defines the internal material chamber and includes an end cap and a plunger. The vent may be formed by a venting passageway in plunger and/or cap in some embodiments. In one embodiment, the vacuum may be created by a medical syringe coupled to the container, and which in some embodiments may also be used to deliver the liquid into the container. The liquid may be bone marrow aspirate in some embodiments. | 05-08-2014 |
| 20140162364 | METHOD FOR PRODUCING A POROUS CALCIUM POLYPHOSPHATE STRUCTURE - A method for producing a porous calcium polyphosphate structure, which comprises the steps of mixing monocalcium phosphate (MCP) with silicic acid, and sintering the mixture at a predefined temperature or temperatures for a predefined time, after which the porous calcium polyphosphate is obtained. The method allows a porous biomaterial with a controllable porosity to be obtained, and which also has the ability to activate the platelets in a plasma rich in platelets and cause the release of growth factors from the platelets. | 06-12-2014 |
| 20140273222 | BIOREACTORS FOR TUBULAR ORGANS - In one embodiment, a bioreactor includes a cartridge adapted to deliver growth media to inner and outer surfaces of a tubular organ scaffold, the cartridge having a container that includes a first passage adapted to deliver a first growth medium to the inner surfaces of the tubular organ scaffold and a second passage adapted to deliver a second growth medium to the outer surfaces of the scaffold, wherein the first and second growth media are different media. | 09-18-2014 |
| 20140287505 | PEPTIDE-PRESENTING SURFACES FOR LONG-TERM CULTURE OF PLURIPOTENT CELLS - The present invention relates to methods of growing and maintaining pluripotent cells on an insoluble substrate that presents a peptide that binds to glycosaminoglycans, such as heparin. Specifically, methods of growing and maintaining pluripotent cells on substrates having a chemically defined surface presenting at least one peptide having basic amino acid residues separated by one or two hydrophobic amino acid residues. | 09-25-2014 |
| 20140315308 | GLUCOMANNAN SCAFFOLDING FOR THREE-DIMENSIONAL TISSUE CULTURE AND ENGINEERING - The present invention provides a neutralized glucomannan scaffold capable of promoting cell growth and suitable for three-dimensional tissue culture and engineering. The present invention also provides methods for making and degrading the neutralized glucomannan scaffold. The present invention further provides a method of growing cells on a neutralized glucomannan scaffold. | 10-23-2014 |
| 20150050735 | WELL PLATES - Well plates for use in various laboratory experiments may include a plurality of wells each having an interior wall that includes a ledge positioned at a fixed depth within the well. | 02-19-2015 |
| 20150104872 | CELL-SUPPORT MATRIX HAVING NARROWLY DEFINED UNIFORMLY VERTICALLY AND NON-RANDOMLY ORGANIZED POROSITY AND PORE DENSITY AND A METHOD FOR PREPARATION THEREOF - A cell-support matrix having narrowly defined uniformly vertically and non-randomly organized porosity and pore density and a method for preparation thereof. The matrix suitable for preparation of cellular or acellular implants for growth and de novo formation of an articular hyaline-like cartilage. A gel-matrix composite system comprising collagen-based matrix having a narrowly defined porosity capable of inducing hyaline-like cartilage production from chondrocytes in vivo and in vitro. | 04-16-2015 |
| 20150307836 | EB Matrix Production from Fetal Tissues and its Use for Tissue Repair - A method of performing and preserving a bioremodelable, biopolymer scaffold material by subjecting animal tissue to chemical and mechanical processing. In addition to skin tissue, another source of EBM is a blood vessel. EBM may be used for hernia repair, colon, rectal, vaginal and or urethral prolapse treatment; pelvic floor reconstruction; muscle flap reinforcement; lung tissue support; rotator cuff repair or replacement; periosteum replacement; dura repair; pericardial membrane repair; soft tissue augmentation; intervertebral disk repair; and periodontal repair. EBM may also be used as a urethral sling, laminectomy barrier or spinal fusion device. | 10-29-2015 |
| 20150336095 | System and Method for a Biomimetic Fluid Processing - A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range. | 11-26-2015 |
| 20150352547 | Isolating Microfluidic Structures and Trapping Bubbles - Some configurations of a microfluidic apparatus can comprise a fluidic circuit of interconnected fluidic structures into which a plurality of different media can be introduced or extracted. A variety of operations can be performed with the different media including isolating with a second medium one or more of the fluidic structures that is filled partially or fully with a first medium. Discrete volumes of a medium can be moved through the isolating second medium to deliver materials or micro-objects to or remove micro-objects or materials from a fluidic structure that is otherwise isolated by the second medium. Some configurations of a microfluidic apparatuses can isolate microfluidic structures in a microfluidic apparatus using flow rates or blocking structures, and some configurations can manage bubbles in fluidic structures. | 12-10-2015 |
| 20160032239 | Deterministic Manufacturing Process For Creating 3D Living Tissues Based on 2D Directed Assembly And Origami Techniques - A method of forming 3D engineered tissues by providing a 2D scaffold material comprising a plurality of fold locations and a plurality of cell assembly sites, assembling cells into the cell assembly sites and folding the 2D scaffold material along the fold locations to form a 3D scaffold structure. Tissues formed by the method. | 02-04-2016 |
| 20160075987 | NOVEL 3D SCAFFOLD MICROSTRUCTURE - A three-dimensional scaffold microstructure comprising a plurality of collapsed polymer columns for use in cell culture or nematode studies. | 03-17-2016 |
| 20160097154 | ENGINEERED LEATHER FOR AIRBAG, STEERING WHEEL, AND SEATBELT APPLICATIONS - Methods of covering portions of an automobile interior with an engineered leather are provided. When a mold of the appropriate shape is provided, a cell paste can be placed therein and grown into a leather, which can be tanned and treated to produce a suitable covering for a portion of an automobile interior, such as a steering wheel, an airbag cover, or a seat belt assembly. A method of growing a seamless automobile component cover in situ is disclosed. | 04-07-2016 |
| 20160108351 | SIMPLE ANIMAL CELL CULTURE DEVICE AND METHOD FOR CULTURING ANIMAL CELLS USING SAME - The present invention relates to a simple animal cell culture device and a method for culturing animal cells using the same, and provides a simple animal cell culture device comprising: a culture vessel for holding a culture medium; an animal cell reservoir coupled to the upper portion of the culture vessel; a cell support housed on the inside of the animal cell reservoir and having animal cells adhered thereto; and a cover for covering the top of the culture vessel. | 04-21-2016 |
| 20160114145 | CONTROLLED OUTGASSING OF HYBERBARICALLY LOADED MATERIALS FOR THE DELIVERY OF OXYGEN AND OTHER THERAPEUTIC GASES IN BIOMEDICAL APPLICATIONS - Devices and methods for delivering oxygen and other therapeutic gases to a target, such as a tissue, a tissue-engineered construct, and a wound, in a controlled and sustained manner are disclosed. | 04-28-2016 |
| 20160137979 | SKIN PRINTING AND AUTO-GRAFTING - A holey substrate now is used for constructing a graft product, such as building an auto-graft by 3D printing of living cells. When the autograft built atop the holey substrate is implanted, blood vessels and other patient tissues can grow through the holes. | 05-19-2016 |
| 20160194603 | Composition and Method for Delivery of Living Cells in a Dry Mode Having a Surface Layer | 07-07-2016 |
| 20160251617 | METHOD FOR PREPARING HETEROGENETIC CORNEAL MATERIAL | 09-01-2016 |
| 20180021780 | SYSTEM AND METHOD FOR A BIOMIMETIC FLUID PROCESSING | 01-25-2018 |
| 20190144819 | INTEGRATED CELLS | 05-16-2019 |
