| Class / Patent application number | Description | Number of patent applications / Date published |
|---|
| 435396000 | Support is a resin | 77 |
| 20080280360 | Method for Producing Biomaterial Scaffolds - The present invention provides a multilayer scaffold for tissue engineering. The scaffold comprises at least a first layer comprised of a polymer having a pattern of microchannels therein; and at least a second layer comprised of a polymer having a pattern of microchannels therein. The first and second layers are joined together (preferably by lamination) and the channels are connected for the circulation of fluid through the layers. The scaffold is coated with bacterial cellulose. The scaffold may further include a mammalian cell. | 11-13-2008 |
| 20080318318 | FILLER-DISPERSED MELT-KNEADED PRODUCTS, MOLDED RESIN PRODUCTS THEREOF, AND PRODUCTION METHOD THEREOF - A melt-kneading method for filling material-containing resin or elastomer includes: a step of preparing a filling material as a filler and a resin or elastomer comprising an incompatible blend; and a step of introducing the filling material-containing resin or elastomer into a material feed part provided at an end of a cylindrical melt-kneading part having a heater and provided with a screw, and then melt-kneading the filling material-containing resin or elastomer under conditions where the rotation speed of the screw is about 600 rpm to about 3,000 rpm and its shear rate is about 900 to about 4,500 sec | 12-25-2008 |
| 20090004738 | Method and apparatus for maintenance and expansion of hemopoietic stem cells and/or progenitor cells - A method of preparing a stromal cell conditioned medium useful in expanding undifferentiated hemopoietic stem cells to increase the number of the hemopoietic stem cells is provided. The method comprising: (a) establishing a stromal cell culture in a stationary phase plug-flow bioreactor under continuous flow on a substrate in the form of a sheet, the substrate including a non-woven fibrous matrix forming a physiologically acceptable three-dimensional network of fibers, thereby expanding undifferentiated hemopoietic stem cells; and (b) when a desired stromal cell density has been achieved, collecting medium from the stationary phase plug-flow bioreactor, thereby obtaining the stromal cell conditioned medium useful in expanding the undifferentiated hemopoietic stem cells. | 01-01-2009 |
| 20090035857 | CELL CULTURE PROCESSING DEVICES AND METHODS - Embodiments are directed to devices and methods for processing, cultivating or otherwise manipulating cell cultures which may be disposed on a flat or substantially flat surface such as cell culture substrate material. Devices and methods are disclosed for dividing a cell culture layer into divided portions, including isolated divided portions, that may then be transferred from the cell culture to a new location. For some embodiments, the divided portions may be transferred to a new cell culture support substrate in order to continue to grow and cultivate the cell line. | 02-05-2009 |
| 20090042294 | Hydroxyphenyl cross-linked macromolecular network and applications thereof - A dihydroxyphenyl cross-linked macromolecular network is provided that is useful in artificial tissue and tissue engineering applications, particularly to provide a synthetic macromolecular network for a wide variety of tissue types. In particular, artificial or synthetic cartilage, vocal cord material, vitreous material, soft tissue material and mitral valve material are described. In an embodiment, the network is composed of tyramine-substituted and cross-linked hyaluronan molecules, wherein cross-linking is achieved via peroxidase-mediated dityramine-linkages that can be performed in vivo. The dityramine bonds provide a stable, coherent hyaluronan-based hydrogel with desired physical properties. | 02-12-2009 |
| 20090047740 | CULTURE AID FOR CELLS AND TISSUES - A biological culture medium provides a three-dimensional framework for cell growth. The medium comprises a film and a matrix. | 02-19-2009 |
| 20090061517 | Cell culture apparatus and methods of making and using same - The present invention provides compositions and articles for cell culture and methods for preparing the compositions and articles. Generally, the article can include a porous biocompatible polymer scaffold. The scaffold may be prepared by preparing a polymer composition that includes a biocompatible polymer and a porogen, then removing the porogen. In some embodiments, the polymer composition may be applied to a substrate. In some embodiments, the polymer composition may be secured to the substrate. | 03-05-2009 |
| 20090098651 | CARDIOMYOCYTE CULTURE SUPPORT - It is an objective of this invention to provide a cardiomyocyte culture support used for obtaining cardiomyocytes having a controlled orientation of beating that are thus available for myocardial regenerative therapy, and to provide a method for producing the same. | 04-16-2009 |
| 20090191633 | Synthetic Surfaces for Culturing Stem Cell Derived Cardiomyocytes - Synthetic surfaces suitable for culturing stem cell derived cardiomyocytes contain acrylate polymers formed from one or more acrylate monomers. The acrylate surfaces, in many cases, are suitable for culturing stem cell derived cardiomyocytes in chemically defined media. | 07-30-2009 |
| 20090246872 | STRUCTURE FOR CELL CULTURE, CELL CULTURE VESSEL, STRUCTURE WITH SPHEROID, VESSEL WITH SPHEROID, AND MANUFACTURING METHODS THEREOF - By using a microfabrication technique such as nanoimprinting, a structure for cell culture comprising: a concavo-convex structure having a plurality of successive unit structures each formed in a polygonal shape in a planar direction and having a minimum internal diameter of less than or equal to 3 μm, wherein a width between adjoining unit structures is less than or equal to 3 μm, a concavo-convex depth is greater than or equal to 10 nm, and the concavo-convex structure functions as a cell adherence surface. By culturing cells in this structure or a cell culture vessel comprising this structure integrated therein, a structure having a spheroid or a vessel having a spheroid, in which a spheroid with lamellipodia has been formed, can be obtained. Thus, it is possible to produce the structure or the cell culture vessel in which the shape, size and material of the peak-and-valley structure are controlled. Furthermore, a spheroid suitable for, e.g., screening a drug can be easily cultured within a short period of time at a low cost. | 10-01-2009 |
| 20090246873 | SCAFFOLD FOR TISSUE ENGINEERING AND PRODUCTION METHOD THEREOF - To provide a scaffold for tissue engineering which consists of a bioabsorbable polymer material to be absorbed in a biotissue, and holds strength of the whole scaffold while having a porosity proper for culturing cells inside thereof as well, a method for producing the scaffold for tissue engineering includes steps of dissolving a bioabsorbable polymer material with an organic solvent, drying the solution so as to produce a porous bioabsorbable polymer material having a porosity of 50 to 99%, covering the porous bioabsorbable polymer material with a bioabsorbable polymer material having a thickness of 0.01 to 5 mm, pores of 10 to 3000 μm diameter, a fracture strength of 0.05 to 0.15 MPa, and a volume of 15 to 90% with respect to the whole scaffold. | 10-01-2009 |
| 20090291500 | PRODUCTION PROCESS OF AN ORGANIC/INORGANIC COMPOSITE HYDROGEL, CELL CULTURE SUBSTRATE COMPRISING AN ORGANIC/INORGANIC COMPOSITE HYDROGEL, AND ANTIFOGGING MATERIAL COMPRISING A DRIED ORGANIC/INORGANIC COMPOSITE HYDROGEL - The present invention provides a production process of an organic/inorganic composite hydrogel, which demonstrates superior mechanical properties, by uniformly dispersing a clay mineral in an organic polymer over a wide range of clay mineral content, and a dried form thereof, to be produced easily in a short period of time. The production process of an organic/inorganic composite hydrogel of the present invention comprises reacting a water-soluble organic monomer (a) in the presence of a water-swellable clay mineral (b) by irradiating with an energy beam in a solution in which a non-water-soluble polymerization initiator (d) is dispersed in an aqueous medium (c). | 11-26-2009 |
| 20090311785 | Method for organizing and controlling cell growth and tissue regeneration - A method for organizing and controlling cell growth on a cell-free structure comprises steps of decellularizing a biological material to produce an acellular matrix; adding cells sought to be propagated to the acellular matrix; coating the acellular matrix with a fiber comprising biological and nonbiological material; and storing the covered acellular matrix for a time sufficient to form organized cell growth in the three-dimensional structural shape. The method may include the step of covering the three-dimensional structural shape by a structural layer to enable generation of a replacement external organ. This structural layer comprises a polymer film and fiber with a biological material and a nonbiological material. The biological material includes a cell growth factor; and the nonbiological material includes magnetite. This structural layer also comprises a therapeutic at a concentration between 0.001 and 5 weight percent. This structural layer may also comprise an acellular matrix of an organ. | 12-17-2009 |
| 20090325296 | ELECTROSPUN ELECTROACTIVE POLYMERS FOR REGENERATIVE MEDICINE APPLICATIONS - Due to the size and complexity of tissues such as the spinal cord and articular cartilage, specialized constructs incorporating cells as well as smart materials may be a promising strategy for achieving functional recovery. Aspects of the present invention describe the use of an electroactive, or piezoelectric, material that will act as a scaffold for stem cell induced tissue repair. Embodiments of the inventive material can also act alone as an electroactive scaffold for repairing tissues. The piezoelectric material of the present invention acts as a highly sensitive mechanoelectrical transducer that will generate charges in response to minute vibrational forces. | 12-31-2009 |
| 20100009448 | Biodegradable and bioabsorbable biomaterials and keratin fibrous articles for medical applications - The present invention relates to a process of making biodegradable and/or bioabsorbable biomaterials and keratin nonwoven fibrous articles by electrospinning fibers from a blend of biomaterials and keratin dissolved in organic solvents includes generating a high voltage electric field between oppositely charged biomaterials and keratin fluid in a syringe with a capillary tip and a metallic collection roller and causing a jet to flow to the roller as solvent evaporates and collecting fibrous membranes or scaffolds on the roller. Keratin increased the cell affinity of biomaterial scaffolds which have potential medical applications. | 01-14-2010 |
| 20100055791 | MATRIX FOR REGENERATING CARDIOVASCULAR TISSUE AND METHOD FOR REGENERATING CARDIOVASCULAR TISSUE - Materials for culturing cardiovascular tissues wherein a sponge made of a bioabsorbable material is reinforced with a reinforcement made of a bioabsorbable material. | 03-04-2010 |
| 20100093093 | MANUFACTURING THREE-DIMENSIONAL SCAFFOLDS USING ELECTROSPINNING AT LOW TEMPERATURES - The present invention refers to an apparatus and a method for the manufacture of a three-dimensional scaffold at low temperatures and the respective use of this method and apparatus. | 04-15-2010 |
| 20100120149 | CELL AGGREGATE-HYDROGEL-POLYMER SCAFFOLD COMPLEX FOR CARTILAGE REGENERATION, METHOD FOR THE PREPARATION THEREOF AND COMPOSITION COMPRISING THE SAME - The present invention relates to a cell aggregate-hydrogel-polymer scaffold complex useful for cartilage regeneration which has a structure in which cell aggregates of differentiated chondrocytes are evenly dispersed in a hydrogel matrix, and the resulting hydrogel matrix is immobilized onto the surface of a polymer scaffold while filling up the pores thereof. Since the cell aggregate-hydrogel-polymer scaffold complex according to the present invention can efficiently induce the regeneration of cartilage tissue similar to natural cartilage and retain high mechanical strength, flexibility, and uniform morphology during the cartilage regeneration, it can be effectively used as a cartilage therapeutic agent for the repair of cartilage defects and injuries. | 05-13-2010 |
| 20100190254 | THREE-DIMENSIONAL POROUS HYBRID SCAFFOLD AND MANUFACTURE THEREOF - The present invention refers to a three-dimensional porous hybrid scaffold for tissue engineering and methods of its manufacture and use. | 07-29-2010 |
| 20100197020 | TISSUE ENGINEERING TENDON AND CONSTRUCTION METHODS IN VITRO THEREOF - The invention discloses a tissue engineering graft, comprising:(a) pharmaceutically-acceptable biodegradable material; and (b) seed cells, which can be inoculated on the described biodegradable material and are selected from:(i) fibroblasts; (ii) adipose derived cells, or (iii) mixture of dermal fibroblasts and ASCs according to the ratio of 1:10000-10000:1. The graft can be prepared by mixing of seed cell and pharmaceutically-acceptable biodegradable material, obtaining a construct of seed cells and pharmaceutically-acceptable biodegradable material, then culturing the construct in a bioreactor in vitro. The graft can be used for repairing the defect of tendon tissues. | 08-05-2010 |
| 20100197021 | KERATIN BIOMATERIALS FOR CELL CULTURE AND METHODS OF USE - Provided are keratin compositions useful in cell culture. In some embodiments the keratins are biocompatible, promote cell growth, promote cell adhesion and provide an excellent substrate for cell culture. Keratin compositions described herein may be used as coatings, gels, three-dimensional scaffolds, additives to cell culture media, microcarriers, etc. The keratin substrates may also be used to deliver cells, e.g., for cell therapy applications. | 08-05-2010 |
| 20100216242 | Cell culture support and production method and uses thereof - The present teachings provide a practical cell culture support by which a cell culture with a high degree of freedom can be realized. More specifically, the cell culture support includes a polymer layer exhibiting thermoresponsiveness and a cell culture region obtained by plasma-treating a surface layer portion thereof with a reactive gas, whereby a cell culture support having thermoresponsiveness and cellular adhesiveness while avoiding or limiting the use of cell adhesion factors is provided. | 08-26-2010 |
| 20100221835 | METHOD FOR CARTILAGE TISSUE REGENERATION VIA SIMULATED MICROGRAVITY CULTURE USING SCAFFOLDS - This invention relates to a method for cartilage tissue engineering using scaffolds in simulated microgravity culture. This invention enables engineering of homogeneous cartilage tissue using bone marrow cells in a more rapid manner in a simulated microgravity environment, while allowing control of the configuration of the resulting cartilage tissue. | 09-02-2010 |
| 20100273260 | CELL CULTURE SUBSTRATE AND PROCESS FOR PRODUCING THE SAME AND METHOD FOR CULTURING CELLS - The present invention relates to a cell culture substrate in which a polymer chain having a hydrophilic skeleton is grafted onto a surface of polystyrene or poly(ε-caprolactone) having a water contact angle of from 75° to 100°. This cell culture substrate has excellent efficiency of cell culture without the necessity of immobilization and adsorption of a cell adhesion substance on a surface of a substrate. | 10-28-2010 |
| 20100273261 | METHODS FOR PRODUCING SYNTHETIC SURFACES THAT MIMIC COLLAGEN COATED SURFACES FOR CELL CULTUE - The present invention discloses methods for producing synthetic surfaces that mimic collagen coated surfaces for cell culture comprising: providing a monomer source comprising one or more organic compounds which are capable of polymerization, wherein at least one organic compound is prolinol; creating a plasma of said monomer source; and contacting at least a portion of a surface with the plasma to provide a plasma polymer coated surface. Advantageously, such methods provide an animal-free, synthetic, chemically defined surface that mimics a collagen coated surface for cell culture. Advantageously, such methods not only reduce the cost and/or issues associated with animal-derived collagen but are also amenable to large scale manufacturing. | 10-28-2010 |
| 20100297768 | NANOFIBRILLAR STRUCTURE AND APPLICATIONS INCLUDING CELL AND TISSUE CULTURE - A nanofibrillar structure for cell culture and tissue engineering is disclosed. The nanofibrillar structure can be used in a variety of applications including methods for proliferating and/or differentiating cells and manufacturing a tissue. Also disclosed is an improved nanofiber comprising a lipid, lipophilic molecule, or chemically modified surface. The nanofibers can be used in a variety of applications including the formation of nanofibrillar structures for cell culture and tissue engineering. | 11-25-2010 |
| 20100317112 | SCAFFOLDS INCREASED SPECIFIC GRAVITY FOR CELL CULTURE AND METHOD FOR MANUFACTURING THEREOF - The present invention relates to microtype scaffolds for cell culture, which have their specific gravity increased and a method for manufacturing thereof, and more specifically, relates to microtype scaffolds for cell culture, which have their specific gravity increased, by adding a chemically stable inorganic compound having a high specific gravity in manufacturing biocompatible polymer microtype scaffolds for cell culture and a method for manufacturing thereof. In case where the inventive microtype scaffolds for cell culture is used, it is easy to separate cells cultured on microtype scaffolds, and cell damage can be minimized by reducing separation time, and it is easy to recover cells due to a definite boundary layer. | 12-16-2010 |
| 20100317113 | SYNTHETIC MICROCARRIERS FOR CULTURING CELLS - A cell culture microcarrier includes a polymer formed from copolymerization of a mixture including (i) an uncharged hydrophilic unsaturated monomer having a hydroxyl group; (ii) a hydrophilic carboxylic acid containing unsaturated monomer; and (iii) a hydrophilic multifunctional unsaturated monomer. The microcarrier may further include a polypeptide, such as a polypeptide that promotes cell adhesion, conjugated to the surface of the microcarrier; e.g. via the carboxyl group from the hydrophilic carboxylic acid containing unsaturated monomer. | 12-16-2010 |
| 20100330674 | CELL CULTURE SUPPORT AND ASSOCIATED METHOD FOR CELL GROWTH AND RELEASE - A cell culture support comprising a substrate, and a dual stimuli responsive block copolymer immobilized on the substrate, wherein the dual stimuli responsive block copolymer is both thermoresponsive and pH responsive. A method of culturing cells comprising the cell culture support having a dual stimuli responsive copolymer immobilized on a substrate, wherein the dual stimuli responsive copolymer is thermoresponsive and pH responsive; and growing the cells on the cell culture support. By lowering the temperature, cells are released from the cell culture support. | 12-30-2010 |
| 20110076771 | TISSUE FIBER SCAFFOLD AND METHOD FOR MAKING - The present disclosure relates to a fiber, a method of forming a fiber, a system for forming a fiber, and a method of engineering tissue from a fiber. The fiber includes an engineered geometric feature forming a non-Euclidian geometry. | 03-31-2011 |
| 20110097801 | TISSUE SCAFFOLDING COMPOSITES - A method for preparing a biocompatible polymeric composite includes modifying a first biocompatible polymer with a primer group to form a modified biocompatible polymer; blending the modified biocompatible polymer with a second biocompatible polymer and an inorganic material; allowing the primer group of the modified biocompatible polymer to react with the inorganic material to form a biocompatible polymeric composite. Such biocompatible polymeric composites may be formed into medical devices such as tissue growth scaffolds and bone growth scaffolds. | 04-28-2011 |
| 20110097802 | ORGANIC-INORGANIC COMPOSITE DISPERSION, CELL CULTURE SUBSTRATE MANUFACTURED USING THE SAME, AND METHODS FOR PREPARING THE SAME - Disclosed are: an organic-inorganic complex dispersion improved in film formability and adhesion to a base material. The organic-inorganic complex dispersion comprises an aqueous medium and particles of a complex dispersed in the aqueous medium, wherein the complex has a three-dimensional network structure formed by a polymer of a monomer comprising a monomer represented by general formula (1) and at least one inorganic material selected from a water-swellable clay mineral and silica. Also disclosed is an antifogging material manufactured by using the organic-inorganic complex dispersion. Further disclosed is a cell culture substratum improved in the detachability of cells cultured on the substratum, which is manufactured by using the organic-inorganic complex dispersion. Still further disclosed are manufacturing methods for same. [In the formula, R1 represents a hydrogen atom or a methyl group; R2 represents an alkylene group having 2 to 3 carbon atoms; R3 represents a hydrogen atom or an alkyl group having 1 to 2 carbon atoms; and n represents a number of 1 to 9.] | 04-28-2011 |
| 20110129924 | Porous Polymeric Articles - Porous polymeric articles, and more specifically, porous polymeric articles for tissue engineering and organ replacement, are described. In some embodiments, methods described herein include use of a polymer-solvent system (e.g., phase inversion) to generate porosity in a structure. The process may include formation of a structure precursor material including a first crosslinkable component and a second component that can be precipitated in a precipitation medium. The structure precursor material may be shaped into a three-dimensional shape by a suitable technique such as three-dimensional printing. Upon shaping of the structure precursor material, at least a portion of the first component may be crosslinked. The structure may then be contacted with a precipitation medium to remove the precursor solvent from the structure, which can cause the second polymer component to precipitate and form a porous structure containing a network of uniform pores. In some embodiments, the porous structure is constructed and arranged for use as a template for ultrafiltration, cell growth, and/or for forming complex, biomimetic, porous biohybrid organs, where living cells can be immobilized and perform their normal physiological functions. | 06-02-2011 |
| 20110151564 | METHOD FOR PROLIFERATION OF CELLS ON POLYELECTROLYTE MULTILAYER FILMS AND USE THEREOF, PARTICULARLY FOR THE PREPARATION OF CELLULAR BIOMATERIALS - The invention relates to the use of a unit including a substrate and polyelectrolyte multilayer films deposited thereon in order to: carry out a method involving the proliferation of initial stem or differentiated cells that are brought into contact with the unit; and cover the unit with confluent viable adherent cells resulting from the proliferation of the initial cells, the cover being obtained at the end of a period of no more than one month, such as 14 days, 11 days or, in particular, 7 days, after the initial cells are brought into contact with the unit. | 06-23-2011 |
| 20110183418 | Peptide-Polymer Cell Culture Articles and Methods of Making - Functionalized peptide monomers, peptides that have been functionalized to contain a polymerization moiety, are disclosed. The use of these functionalized peptide monomers to form peptide polymers which are useful as synthetic surfaces capable of supporting culture of cells in culture, particularly cells that will be used therapeutically, is also disclosed. Methods of making the surfaces and methods of using the surfaces are also disclosed. | 07-28-2011 |
| 20110217775 | METHODS FOR MODULATING OSTEOCHONDRAL DEVELOPMENT USING BIOELECTRICAL STIMULATION - Compositions and methods are provided for modulating the growth, development and repair of bone, cartilage or other connective tissue. Devices and stimulus waveforms are provided to differentially modulate the behavior of osteoblasts, chondrocytes and other connective tissue cells to promote proliferation, differentiation, matrix formation or mineralization for in vitro or in vivo applications. Continuous-mode and pulse-burst-mode stimulation of cells with charge-balanced signals may be used. Bone, cartilage and other connective tissue growth is stimulated in part by nitric oxide release through electrical stimulation and may be modulated through co-administration of NO donors and NO synthase inhibitors. Bone, cartilage and other connective tissue growth is stimulated in part by release of BMP-2 and BMP-7 in response to electrical stimulation to promote differentiation of cells. The methods and devices described are useful in promoting repair of bone fractures, cartilage and connective tissue repair as well as for engineering tissue for transplantation. | 09-08-2011 |
| 20110244572 | FLAT MICROFIBERS AS MATRICES FOR CELL GROWTH - The present invention relates to culturing cells utilizing a matrix of microfibrillated thermoplastic polymeric materials. More specifically, the present invention relates to a method of culturing cells. In addition, the invention relates to a microfibrillated article for culturing cells dispersed in a cell culture medium. The matrix of thermoplastic polymeric materials for culturing cells of this invention finds use in tissue engineering and wound healing applications. | 10-06-2011 |
| 20110256628 | ADAPTIVE TISSUE ENGINEERING SCAFFOLD - The embodiments described herein include porous scaffolds formed from a stimuli-responsive polymer. The stimuli-responsive polymer of the scaffold creates a “smart” scaffold that changes properties in response to an effective stimulus applied to the stimuli-responsive polymer. In a preferred embodiment, an effective stimulus applied to the scaffold initiates a phase transition event in the stimuli-responsive polymer that results in a change in the volume of the pores of the scaffold. The scaffolds can be used to capture appropriately sized objects (e.g., cells) by using the volume-change properties of the pores. Relatedly, the scaffolds can be used as tissue-engineering scaffolds by capturing cells in the pores and introducing the cell-loaded scaffold into a cell-growth environment (e.g., in vivo). | 10-20-2011 |
| 20110263020 | MEMBRANE FOR CELL EXPANSION - A membrane which can be used for cultivating cells, in particular adherent cells. The membrane permits the adhesion and proliferation of the cells based on its specific composition comprising polyurethane. The resulting surface characteristics further permit the membrane to be used without any pre-treatment with surface modifying substances. A method for preparing a membrane which can be used for cultivating cells, in particular adherent cells. Methods of using the membrane for cultivating cells, in particular adherent cells. | 10-27-2011 |
| 20120015440 | SPHEROID COMPOSITE, SPHEROID-CONTAINING HYDROGEL AND PROCESSES FOR PRODUCTION OF SAME - A spheroid composite includes: a substrate including a cell-adhesive porous base material and plural hydrophilic regions and hydrophobic regions that are disposed on the porous base material and formed by curing a photosensitive composition, wherein the photosensitive composition includes a branched polyalkylene glycol derivative having three or more polyalkylene glycol groups, each having a polymerizable substituent at a terminal thereof, and a tri- or higher-valent linking group that binds to the polyalkylene glycol groups; and spheroids formed in the hydrophobic regions on the substrate, the plural spheroids having a uniform size. A spheroid-containing hydrogel, which includes a hydrogel and two or more spheroids having a uniform size with a diameter of from 70 μm to 400 μm that are disposed in the hydrogel in such a manner that the two or more spheroids do not contact each other, can favorably maintain the function of the plural spheroids contained within the hydrogel. | 01-19-2012 |
| 20120040461 | FABRICATION OF NANOFIBER REINFORCED STRUCTURES FOR TISSUE ENGINEERING - Disclosed are composite arrays and methods of forming the arrays. Composite arrays include a hydrogel-forming polymeric network and a network of electrospun fibers embedded within the polymeric network. For instance, the polymeric network can include one or more extracellular matrix proteins. The network of electrospun fibers can describe an open configuration that incorporates sufficient space between adjacent fibers to allow for cellular ingrowth between and among individual fibers. Disclosed composite arrays can be utilized as a supporting scaffold for living cells, for instance in development of bioengineered tissue constructs. | 02-16-2012 |
| 20120122219 | POROUS COMPOSITE BIOMATERIALS AND PRODUCTION METHOD OF THE SAME - The invention discloses a porous composite biomaterial comprising of poly(γ-glutamic acid)-g-chondroitin sulfate (γ-PGA-g-CS) copolymer and poly(ε-caprolactone). The composite biomaterial provides a three-dimensional microenviroment for using as a scaffold for tissue engineering and for supporting the attachment and proliferation of cells. The invention also discloses a method of producing a porous composite biomaterial. | 05-17-2012 |
| 20120156780 | Polymer Substrates Having Improved Biological Response From HKDCS - A method of surface modification of a biocompatible, biodegradable polymer substrate using RF plasma treatment is disclosed. This method and the resulting surface provide for enhanced adhesion and proliferation of cells, such as hKDCs, and can be used with scaffolds for tissue regeneration and with other delivery vehicles such as medical devices. | 06-21-2012 |
| 20120156781 | TEMPERATURE-RESPONSIVE CELL CULTURE SUBSTRATE ON WHICH A STRAIGHT-CHAIN TEMPERATURE-RESPONSIVE POLYMER IS IMMOBILIZED, AND MANUFACTURING METHOD THEREFOR - Provided is a temperature-responsive cell culture substrate. A non-crosslinked temperature-responsive polymer having a molecular weight between 10,000 and 150,000 is immobilized on the substrate surface with a density of 0.02 to 0.3 molecular chain per square nanometer. Using the provided temperature-responsive cell culture substrate, cells obtained from various tissues can be efficiently cultured. This culturing method makes it possible to efficiently peel off a cell sheet by just changing the temperature, without causing damage. | 06-21-2012 |
| 20120178165 | POLYMERIC COATINGS AND METHODS FOR FORMING THEM - The present invention relates to a controllable polymeric surface coating including a macromolecule, which is covalently bound to the surface of a substrate, the macromolecule including a plurality of polymerisation initiators and a plurality of surface binding groups. Pendant polymers may be grafted from at least some of the polymerisation initiators. | 07-12-2012 |
| 20120282697 | SYNTHETIC COMPOSITION AND COATING FOR CELL CULTURE - A composition for forming a polymeric cell culture surface includes (i) a pre-polymer comprising a polymer backbone, a cationic moiety conjugated to the backbone, and a cross-linker moiety conjugated to the backbone; and (ii) a peptide-polymer comprising a polymer backbone and cell adhesive peptide conjugated to the backbone. Cross-linked coatings for cell culture that have a suitable amount of cell adhesive peptide and cationic moiety may be formed from the pre-polymer and peptide-polymer. | 11-08-2012 |
| 20120288939 | NANOPARTICULATE CELL CULTURE SURFACE - A cell culture article including a substrate having nanoparticles on the substrate surface, the nanoparticle including:
| 11-15-2012 |
| 20130029421 | Methods for Making Cell Culture Substrates - Cell culture substrates are provided. Aspects of the cell culture substrate include a substrate with a surface having at least one hydrophilic region and at least one hydrophobic region, and a surfactant layer present on the surface of the substrate and configured to produce a cell-binding surface on the hydrophilic regions of the surface. Also provided are kits which include the cell culture substrate, as well as methods of producing the cell culture substrate. The cell culture substrate and methods described herein find use in a variety of applications, including single-cell culture applications. | 01-31-2013 |
| 20130143325 | BIOCOMPATIBLE POLY (AMIC ACID) AND METHOD OF PREPARATION THEREOF - A method is provided for the preparation of a poly(amic acid) in which ring opening polymerization is employed to react the monomers ethylenediaminetetraacetic dianhydride and paraphenylenediamine in an aprotic solvent. The resulting poly(amic acid) composition is suitable as a biocompatible material, such as a biomedical implant, implant coating material, tissue scaffold material, controlled release drug delivery vehicle, and cellular growth substrate. | 06-06-2013 |
| 20130210148 | CURVED AND FLEXIBLE MICROFLUIDICS - A method of producing curved, folded or reconfigurable structures includes providing a polymer film, exposing the polymer film to at least one of patterned radiation or patterned chemical contact, and conditioning the polymer film subsequent to the exposing. The polymer film includes a polymer that is active to cross-linking of polymer chains in response to the exposing. The exposing is performed such that at least one exposed region of the polymer film develops a gradient in an amount of cross-linking of polymer chains along a cross-sectional direction of the polymer film, and the conditioning of the polymer film removes uncross-linked polymer chains to provide a curved, folded or reconfigurable structure. | 08-15-2013 |
| 20130224860 | THREE-DIMENSIONAL FIBROUS SCAFFOLDS FOR CELL CULTURE - Provided herein is a three-dimensional scaffold composition comprising randomly oriented fibers, wherein the fibers comprise a polyethylene glycol-polylactic acid block copolymer (PEG-PLA) and a poly(lactic-co-glycolic acid) (PLGA). Also provided are methods for using the three-dimensional scaffolds described herein. | 08-29-2013 |
| 20130344601 | MICRO-STRUCTURED BIOMATERIALS AND FABRICATION METHODS THEREFOR - Techniques, systems, apparatus and material are disclosed for fabricating a micro-structured biomaterial. In one aspect, a micro-structured biomaterial includes a three-dimensional solid-phase micro-cellular biomaterial that exhibits a negative Poisson ratio that is tunable in magnitude. | 12-26-2013 |
| 20140093962 | NON-ADHERENT CELL SUPPORT AND MANUFACTURING METHOD - A non-adherent cell support for use as a substrate in fluidic chambers used for cell culturing and assays. The non-adherent cell support allows for the formation of sphere cultures from single cells, which can better mimic primary tumor-like behavior in the study of cancer stem cells. The non-adherent cell support can allow for adhesive culturing and may include a hydrophobic substrate having a lower body and a raised support structure extending upwardly from an upper surface of the body. The support structure comprises one or more vertically extending support members that extend from a proximal portion at the upper surface of the body to a distal end spaced from the upper surface of the body. The support structure may be formed from a biocompatible material such as poly-2-hydroxyethyl methacrylate, polydimethylsiloxane, polymethyl methacrylate, polystyrene, or a polyethylene glycol diacrylate-based hydrogel. | 04-03-2014 |
| 20140106454 | METHOD FOR PRODUCING THREE-DIMENSIONAL MONOLITHIC MICROFLUIDIC DEVICES - A method is described for producing a microfluidic device ( | 04-17-2014 |
| 20140193911 | THERMORESPONSIVE CELL CULTURE SUPPORTS - The present invention relates to a cell culture support comprising a substrate and a thermoresponsive polymeric blend layer, wherein the polymeric blend layer comprises at least one thermoresponsive polymer and at least one network forming adhesion promoter. The present invention further relates a method of making a cell culture complex comprising: providing a substrate; blending at least one thermoresponsive polymer and at least one network forming adhesion promoter to provide a polymeric blend; applying a thin film of said polymeric blend to the substrate to provide a polymeric blend layer on the substrate; curing the polymeric blend layer on the substrate to provide a cell culture support; and depositing cells onto said cell culture support, wherein the cells may optionally further comprise medium, to provide a cell culture complex. | 07-10-2014 |
| 20140199764 | MICROFLUIDIC MODULE AND USES THEREOF - Described herein are microfluidic modules and methods for making the same, wherein the microfluidic modules include a substrate comprising at least one ether-based, aliphatic polyurethane, and at least one fluidic element disposed therein. The ether-based aliphatic polyurethane can be either the substrate of the microfluidic modules or a coating of another substrate material, such that at least a portion of the ether-based, aliphatic polyurethane is in fluid communication. In one embodiment, the ether-based, aliphatic polyurethane includes dicyclohexylmethane-4,4′-diisocyanate. As the ether-based aliphatic polyurethane can decrease absorption of molecules, e.g., hydrophobic molecules, in such microfluidic modules, the microfluidic modules described herein can be used in various applications such as drug screening and fluorescent microscopy. | 07-17-2014 |
| 20140212973 | TEMPERATURE-RESPONSIVE SUBSTRATE FOR CELL CULTURE AND PRODUCTION METHOD THEREOF - To form a temperature-responsive surface for cell culture by simple processes, said temperature-responsive surface for cell culture being capable of efficiently culturing cells. Cultured cells or a cell sheet can be efficiently removed from the temperature-responsive surface for cell culture by merely changing the temperature of the substrate surface. To coat the substrate surface with a block copolymer, in which a water insoluble polymer segment is coupled with a temperature-responsive polymer segment, in an amount of 0.8 to 3.0 μg/cm | 07-31-2014 |
| 20140273223 | MICRO-DEVICE FOR CULTURING CELLS, METHOD FOR MANUFACTURING SAME, AND METHOD FOR CULTURING CELLS USING THE MICRO-DEVICE FOR CULTURING CELLS - Disclosed is micro-device for culturing cells comprising: a plurality of fluid paths through which fluid moves; and at least one inlet port for injecting fluid to the fluid paths, said fluid paths communicating with each other and being different in height from each other. In the cell culture device having a plurality of fluid paths, cells can be cultured by introducing a polymeric material to at least one fluid path having a relatively low height; solidifying the polymeric material to form a 3-dimensional scaffold; and injecting fluid for cell culture to a fluid path in contact with the 3-dimensional scaffold. | 09-18-2014 |
| 20140287506 | DEFINED CELL CULTURING SURFACES AND METHODS OF USE - In one aspect, there is provided a cell culturing substrate including: | 09-25-2014 |
| 20150017725 | PRODUCTION METHOD OF POLYURETHANE POROUS MEMBRANE TO BE USED FOR AT LEAST ONE OF APPLICATIONS OF CELL CULTURE AND CANCER CELL GROWTH INHIBITION - A polyurethane porous membrane is produced by a simple method to be used for at least one of applications of cell culture and cancer cell growth inhibition. The production method of the polyurethane porous membrane to be used for at least one of the applications of cell culture and cancer cell growth inhibition comprises: a first step of forming a layer of a polyurethane material which is uncured, on a substrate; and a second step of supplying water vapor to an exposed surface of the layer of the polyurethane material formed on the substrate, which is away from the substrate, so as to cure the polyurethane material and provide the layer of the polyurethane material with a porous structure having a plurality of irregularities on the exposed surface. | 01-15-2015 |
| 20150024493 | METHOD FOR PREPARING A PATTERNED SUBSTRATE AND USE THEREOF IN IMPLANTS FOR TISSUE ENGINEERING - A method for preparing a patterned substrate is provided. The method includes melt-spinning at least one biocompatible polymer to form fibers; collecting the fibers on a substrate such that the fibers are aligned on the substrate; and applying a binding agent to the aligned fibers to bond the fibers into the aligned arrangement to obtain the patterned substrate in form of an aligned fiber mat. Use of the patterned substrate in an implant for tissue engineering is also provided. | 01-22-2015 |
| 20150024494 | DEFINED CELL CULTURING SURFACES AND METHODS OF USE - In one aspect, there is provided a cell culturing substrate including:
| 01-22-2015 |
| 20150031131 | TISSUE-ENGINEERED CONSTRUCTS - The present invention provides constructs including a tubular biodegradable polyglycolic acid scaffold, wherein the scaffold may be coated with extracellular matrix proteins and substantially acellular. The constructs can be utilized as an arteriovenous graft, a coronary graft, a peripheral artery bypass conduit, or a urinary conduit. The present invention also provides methods of producing such constructs. | 01-29-2015 |
| 20150050736 | Hybrid Tissue Scaffold For Tissue Engineering - A hybrid tissue scaffold is provided which comprises a porous primary scaffold having a plurality of pores and a porous secondary scaffold having a plurality of pores, wherein the secondary scaffold resides in the pores of the primary scaffold to provide a hybrid scaffold. The pores of the porous primary scaffold may have a pore size in a range of 0.50 mm to 5.0 mm, and the pores of the porous secondary scaffold may have a pore size in a range of 50 μm to 600 μm. The primary scaffold may provide 5% to 30% of a volume of the hybrid scaffold. | 02-19-2015 |
| 20150072429 | Method For Manufacturing A Three-Dimensional Biomimetic Scaffold And Uses Thereof - The present invention relates to a method for manufacturing a three-dimensional (3D) biomimetic scaffold that exploits the use of electrical fields and electrical insulating materials to pattern previously polymerized hydro gels with different molecules and/or macromolecular entities. The invention also relates to the 3D-biomimetic scaffolds obtained and to the uses and applications thereof. | 03-12-2015 |
| 20150072430 | MICRO AND NANO SCALE STRUCTURES DISPOSED IN A MATERIAL SO AS TO PRESENT MICROMETER AND NANOMETER SCALE CURVATURE AND STIFFNESS PATTERNS FOR USE IN CELL AND TISSUE CULTURING AND IN OTHER SURFACE AND INTERFACE APPLICATIONS - A structure for use in cell and tissue culturing and in other surface and interface applications. The structure comprises a first material layer defining one or more surface features therein disposed randomly or in a pattern, the one or more surface features having the same or different sizes and cross sectional shapes, a second material layer disposed in or on the one or more surface features, a microstructure disposed in or on the one or more surface features and at least partially embedded and immobile within the second material layer, the microstructure presenting a curvature and a stiffness value and protruding above an upper surface of the second material, a size of the microstructure between 1 nanometer and 10 millimeters, and the structure for use in cell and tissue culturing and in other surface and interface applications wherein a cell grows on the microstructure. | 03-12-2015 |
| 20150087062 | System and Method for Electrospun Biodegradable Scaffold for Bone Repair - This invention relates a structure and system for growth factor incorporation which can improve the osteogenic differentiation of hMSCs, for potential bone regeneration and bone growth applications or used alone for bone repair or growth applications. The system comprises a biodegradable polyester, a hydrophilic polymer, a growth factor and optionally a bioceramic. | 03-26-2015 |
| 20150140659 | THREE-DIMENSIONAL CELL CULTURE SYSTEM AND MANUFACTURING METHOD THEREOF - A three-dimensional cell culture system for an imaging system to observe a cell image includes at least two cell culture layers formed by a solution having a photo-polymerizable monomer, a bio-molecule, an acoustic scattering medium solution and a cell culture medium. After placing a cell into the two cell culture layers, the two cell culture layers are laminated to form a three-dimensional culture laminating layer for culturing the cell. After forming the three-dimensional culture laminating layer, at least one cell-locating layer having a polyethylene glycol diacrylate (PEGDA) solution, the acoustic scattering medium solution, a plurality of photoacoustic markers and the cell culture medium is positioned into the three-dimensional culture laminating layer so as to form the three-dimensional cell culture system. The imaging system is constructed according to a theory selected from one of optics, acoustics, optoacoutics and acousto-optics. | 05-21-2015 |
| 20150140660 | HYDROGEN CYANIDE-BASED POLYMER SURFACE COATINGS AND HYDROGELS - The present invention provides a process of coating at least a portion of a substrate surface comprising contacting the surface with hydrogen cyanide monomeric units under conditions permitting polymerisation of the hydrogen cyanide monomeric units to form a polymer that coats the surface. Also provided is a substrate coated by a polymer according to the claimed process. Also provided is a method of forming a hydrocyanic acid-based hydrogel, the method comprising co-polymerisation in a solution, the solution comprising hydrogen cyanide monomer units and co-monomers. | 05-21-2015 |
| 20150367035 | ELASTIC MACRO POROUS SCAFFOLD AND A PROCESS FOR THE PREPARATION THEREOF - The present invention discloses elastic macro porous scaffold and a process for the preparation thereof. The present invention also provides a process for the preparation of macroporous, elastic nano particulate scaffolds comprising of coated or grafted cross linkable nanoparticles, and a crosslinker prepared by crosslinking during ice templating, wherein the modulus increases linearly with temperature. | 12-24-2015 |
| 20160040121 | THERMALLY RESPONSIVE CELL CULTURE SURFACES - A stimuli responsive nanofiber that includes a stimuli responsive polymer, such as a thermally responsive polymer, and a cross-linking agent having at least two latent reactive activatable groups. The nanofiber may also include a biologically active material or a functional polymer. The stimuli responsive nanofiber can be used to modify the surface of a substrate. When the nanofiber includes a thermally responsive polymer, the physical properties of the surface can be controlled by controlling the temperature of the system, thus controlling the ability of the surface to bind to a biologically active material of interest. | 02-11-2016 |
| 20160102167 | COMB-LIKE POLYURETHANE AND METHODS FOR PREPARING AND USING THE SAME - A method for preparing a comb-like polyurethane, including: 1) adding a diol to a reaction vessel, stirring, heating, vacuum dehydrating, and cooling the diol; adding a diisocyanate to the diol, and pre-polymerizing the diisocyanate and the diol under vacuum to yield a polyurethane prepolymer; 2) dissolving lysine in a mixture of water and an organic solvent to yield a first solution, adding the first solution to the polyurethane prepolymer to yield a first reaction mixture; stopping stirring and allowing the first reaction mixture to stand for between 10 and 12 hrs, pouring the first reaction mixture into water, and drying a precipitate to yield a polyurethane elastomer containing carboxyl groups; 3) dissolving the polyurethane elastomer in an organic solvent to yield a second solution; adding an epoxy-terminated polyethylene glycol to the second solution, and stirring a resulting mixture at between 110 and 130° C. | 04-14-2016 |
| 20160376550 | CELL CULTURE SUPPORT AND ASSOCIATED METHOD FOR CELL GROWTH AND RELEASE - A cell culture support comprising a substrate, and a dual stimuli responsive block copolymer immobilized on the substrate, wherein the dual stimuli responsive block copolymer is both thermoresponsive and pH responsive. A method of culturing cells comprising the cell culture support having a dual stimuli responsive copolymer immobilized on a substrate, wherein the dual stimuli responsive copolymer is thermoresponsive and pH responsive; and growing the cells on the cell culture support. By lowering the temperature, cells are released from the cell culture support. | 12-29-2016 |
| 20170233696 | MEDICAL INSTRUMENT, CELL CULTURE METHOD, FLUORINE-CONTAINING CYCLIC OLEFIN POLYMER AND FLUORINE-CONTAINING CYCLIC OLEFIN POLYMER COMPOSITION FOR IT, AND CULTURED CELLLS | 08-17-2017 |
| 20180023050 | COMPOSITIONS AND METHODS OF CELL ATTACHMENT | 01-25-2018 |
| 20180023053 | Synthetic Surfaces For Culturing Stem Cell Derived Oligodendrocyte Progenitor Cells | 01-25-2018 |
| 20190144818 | HIERARCHICALLY STRUCTURED PROTEIN MATERIALS FOR THREE DIMENSIONAL (3D) CELLULAR SUPPORT SYSTEMS | 05-16-2019 |
