| Entries |
| Document | Title | Date |
|---|
| 20080213821 | Microfluidic Cell Sorter System - A microfluidic system for separating, purifying and counting cell sub-populations, utilising steering of liquid flows in microfluidic channels in a cell focusing region (first dotted circle area); having the integration of the optical detection mechanism and a microchannel structure made from moulding. A master is photolithographically patterned on a soft PDMS silicon or polymer material. After being moulded and peeled off the master, the micro-channel structure is sealed on a hard substrate with openings punched through for wells ( | 09-04-2008 |
| 20080213822 | METHODS FOR DIAGNOSING CELIAC SPRUE AND REAGENTS USEFUL THEREIN - Administering an effective dose of glutenase to a Celiac or dermatitis herpetiformis patient reduces levels of toxic gluten oligopeptides, thereby attenuating or eliminating the damaging effects of gluten. | 09-04-2008 |
| 20080213823 | CAPILLARY-CHANNELED POLYMER FILM FLOW CYTOMETRY - The present invention relates to methods for counting, sorting, and manipulation of cells, organelles, and/or cellular material using capillary-channeled polymer films. | 09-04-2008 |
| 20080241875 | APPARATUS AND METHOD OF DETECTING MICROORGANISM OR MICRO-PARTICLE IN REAL TIME - An apparatus for detecting a microorganism or micro-particle in real time includes a collection module comprising a condensation element unit which condenses water particles in an atmosphere and forms a droplet to which a microorganism or micro-particle in the atmosphere adheres to, and a collection channel unit which gathers the droplet and generates a droplet stream and a sensing module including a counting module. The droplet stream is introduced to the sensing module, and the sensing module detects and counts the microorganism or micro-particle which is adhered to the introduced droplet stream. | 10-02-2008 |
| 20080268494 | HEMATOLOGY ANALYZER, HEMATOLOGY ANALYZING METHOD, AND COMPUTER PROGRAM PRODUCT - A hematology analyzer, obtains red blood cell scattered light information which is scattered light information related to red blood cells contained in a blood sample and reticulocyte scattered light information which is scattered light information related to reticulocytes contained in the blood sample; obtains a value equivalent to the amount of hemoglobin in the red blood cells from the red blood cell scattered light information; obtains a value equivalent to the amount of hemoglobin in the reticulocytes from the reticulocyte scattered light information; obtains difference between the hemoglobin amounts which is the difference between the value equivalent to the amount of hemoglobin in the red blood cells and the value equivalent to the amount of hemoglobin in the reticulocytes; and obtains information supporting clinical examination based on the value equivalent to the amount of hemoglobin in the reticulocytes and the difference between the hemoglobin amounts, is disclosed. A hematology analyzing method and a computer program product are also disclosed. | 10-30-2008 |
| 20080318268 | Devices and Methods for the Selection of Agents with Efficacy Against Biofilm - This invention is a diagnostic plate that can be used to select antibiotic combinations with efficacy against microorganisms growing as a biofilm. The plate allows growth of biofilm on a plurality of projections, and the subsequent simultaneous challenge of biofilms on all projections of the plate to independent concentrations and combinations of anti-biofilm agents. Resistance of microorganisms to antibiotics is higher when they grow as a biofilm, as compared to when they grow in a planktonic state which is usually used to determine their level of antibiotic sensitivity. Growth of microorganisms that slough off the biofilm in the anti-biofilm agent challenge determines the Minimum Inhibitory Concentration (MIC) which relates to sensitivity of the microorganisms in a planktonic state. Growth of any surviving microorganisms from the biofilm in a subsequent recovery step determines the Minimal Biofilm Eradication Concentration (MBEC) which relates to the sensitivity of the microorganisms growing as a biofilm. Enumeration of the surviving microorganisms in the recovery step determines the Minimum Biocidal Concentration (MBC). | 12-25-2008 |
| 20080318269 | Devices and Methods for the Analysis of Biofilm - This invention is a diagnostic plate that can be used to select antibiotic combinations with efficacy against microorganisms growing as a biofilm. The plate allows growth of biofilm on a plurality of projections, and the subsequent simultaneous challenge of biofilms on all projections of the plate to independent concentrations and combinations of anti-biofilm agents. Resistance of microorganisms to antibiotics is higher when they grow as a biofilm, as compared to when they grow in a planktonic state which is usually used to determine their level of antibiotic sensitivity. Growth of microorganims that slough off the biofilm in the anti-biofilm agent challenge determines the Minimum Inhibitory Concentration (MIC) which relates to sensitivity of the microorganisms in a planktonic state. Growth of any surviving microorganims from the biofilm in a subsequent recovery step determines the Minimal Biofilm Eradication Concentration (MBEC) which relates to the sensitivity of the microorganisms growing as a biofilm. Enumeration of the surviving microorganims in the recovery step determines the Minimum Biocidal Concentration (MBC). | 12-25-2008 |
| 20080318270 | MASKING BACKGROUND FLUORESCENCE AND LUMINESCENCE IN THE OPTICAL ANALYSIS OF BIOMEDICAL ASSAYS - In a process for the quantitative optical analysis of fluorescently labelled biological cells | 12-25-2008 |
| 20090011458 | Method for selectively staining microorganisms - A method for selectively flagging target microorganisms in a liquid sample also including background particles comprises the steps of adding a lysing agent selected to breach the background particles to the sample, adding a dye selected to flag the target microorganisms to the sample, adding a suppressing agent selected to penetrate the breached background particles and suppress the dye within the breached background particles to the sample, and measuring the flagged target microorganisms in the sample. The method is particularly useful in organic samples such as dairy and blood product solutions. | 01-08-2009 |
| 20090029409 | METHOD FOR DETERMINATION OF OXIDATIVE STRESS - Provided is a biomarker that enables easy and rapid detection of oxidative stress on a living organism and enables prevention of tissue damage or cell necrosis by drug administration, and which is a powerful marker for the study of toxicity and pharmacokinetics of various agents. Oxidative stress is determined by measuring blood concentration of ophthalmic acid, which is a substance that varies in blood depending on the variation of reduced glutathione (GSH) concentration in a biological sample with the use of an analyzer such as a capillary electrophoresis-mass spectrometer. Further, an anti-oxidative stress agent is screened by administering an anti-oxidative stress candidate agent to a non-human animal under oxidative stress conditions, measuring blood concentration of ophthalmic acid, and evaluating the degree of decrease in the ophthalmic acid concentration. | 01-29-2009 |
| 20090035808 | MEDIUM FOR DETECTING MICROORGANISMS - Provided is a culture medium for microorganisms present in contaminated working fluids such as coolants. More particularly, said culture medium is particularly suitable for supporting growth of microorganisms colonizing metalworking fluids and allows for specific detection of both bacterial microorganisms and fungal microorganisms the latter depending on the added selective agents which can be antibiotics for the detection of fungal contamination or fungicides for the detection of bacterial contamination. Furthermore, devices and kits comprising the culture medium of the present invention are described as well as a method of detecting microbial contamination of metalworking fluids. | 02-05-2009 |
| 20090061476 | Method and apparatus for imaging target components in a biological sample using permanent magnets - A system for enumeration of cells in fluids by image cytometry is described for assessment of target populations such as leukocyte subsets in different bodily fluids or bacterial contamination in environmental samples, food products and bodily fluids. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. A small, permanent magnet is inserted directly into the chamber containing the labeled sample. The magnets are coated with PDMS silicone rubber to provide a smooth and even surface which allows imaging on a single focal plane. The cells are illuminated and the images of the fluorescent light emitted by the target cells are captured by a CCD camera. Image analysis performed with a novel algorithm provides a count of the cells on the surface that can be related to the target cell concentration of the original sample. | 03-05-2009 |
| 20090061477 | Method and apparatus for imaging target components in a biological sample using permanent magnets - A system for enumeration of cells in fluids by image cytometry is described for assessment of target populations such as leukocyte subsets in different bodily fluids or bacterial contamination in environmental samples, food products and bodily fluids. Briefly, fluorescently labeled target cells are linked to magnetic particles or beads. In one embodiment, a small, permanent magnet is inserted directly into the chamber containing the labeled cells. The magnets are coated with PDMS silicone rubber to provide a smooth and even surface which allows imaging on a single focal plane. The magnet is removed from the sample and illuminated with fluorescent light emitted by the target cells captured by a CCD camera. In another embodiment, a floater having a permanent magnet allows the target cells to line up along a single imaging plane within the sample solution. Image analysis can be performed with a novel algorithm to provide a count of the cells on the surface, reflecting the target cell concentration of the original sample. | 03-05-2009 |
| 20090061478 | High-Speed Quantification of Antigen Specific T-Cells in Whole Blood by Flow Cytometry - The present invention discloses novel methods and compositions for identifying particular cell types in whole blood samples and defining either the concentration or ‘absolute count’ of these cells per unit volume of the sample. More specifically, the invention relates to methods for quantifying the antigen-specific T cells or defining the relative percentage of said cells in un-lysed whole blood. Further, the invention relates to kits for the preparation of whole blood samples for high-speed quantification of particular cell types, e.g. antigen specific T-cells, in said whole blood samples by flow cytometry. | 03-05-2009 |
| 20090075324 | Method and System for Quantitative Hemoglobin Determination - A method for quantitative hemoglobin determination in undiluted, unhemolyzed whole blood is provided which comprises: acquiring a sample of unaltered whole blood into a capillary cuvette, presenting the cuvette to a set-up for an absorption measurement, delaying absorption measurement for a determined period of time, performing a first absorption measurement at a first wavelength in the range 490-520 nm directly on the sample in the cuvette, further conducting a second absorption measurement at a second wavelength different from the first wavelength and at which the absorption is substantially smaller than at the first wavelength, and processing results of the first and second absorption measurements to determine the concentration of hemoglobin in the sample. A system for implementing the method also is provided. | 03-19-2009 |
| 20090098597 | METHOD FOR QUANTIFYING CELL MOTILITY AND CELL MIGRATION - A method of quantifying cell migration of a cell population is provided. The method includes the step of patterning the cell population within a channel network in a first body. A first image of the cell population is obtained. Thereafter, a second image of the cell population is obtained after a first predetermined time period. The first and second images are compared in order to calculate a quantitative measure of the average directional migration of the cells population and a quantitative measure of the average motility of the cell population. | 04-16-2009 |
| 20090104653 | Bio-process model predictions from optical loss measurements - This invention relates to methods for monitoring and controlling bioprocesses. Specifically, it describes using quasi-real-time analytical and numerical techniques to analyze optical loss measurements calibrated to indicate cell viability, whereby it is possible to reveal process changes and/or process events such as feeding or induction. Additionally, the present invention makes it possible to accurately estimate the onset of a decrease in cell viability and/or a suitable time for cell harvesting for a cell culture growth process. Pattern recognition methods for identifying specific process events such as batch feeding, cell infection, and product precipitation are also described. | 04-23-2009 |
| 20090136991 | Identifying Naive, Infected, or Vaccinated Mammals - This document provides methods and materials related to assessing a mammal's immunological state. For example, methods and materials related to assessing a mammal to determine whether the mammal is immunologically naïve with respect to a pathogen, was vaccinated against that pathogen, or is infected with that pathogen are provided. This document also provides methods and materials that can be used to determine whether or not a mammal (e.g., a cow) has an early stage infection (e.g., stage 1, Johne's disease). | 05-28-2009 |
| 20090155840 | METHOD AND DEVICE FOR CELL COUNTING - A microfluidic device and method is provided for determining a cell concentration in a sample. The microfluidic device includes a body having a channel therethough that extends along an axis. The channel includes an input and an output, and is at least partially defined by a surface. Indicia overlaps the surface. The channel has a predetermined volume. A portion of the sample is provided in the channel and the cells in the predetermined portions of the channel defined by the indicia are counted. | 06-18-2009 |
| 20090176269 | CELL CULTURE PROCESSES - Culturing heterologous protein-secreting mammalian cells, such as CHO or BHK cells, at 35.1-36.5° C. and/or at pH 7.15-7.20 and/or at a dissolved CO | 07-09-2009 |
| 20090181421 | DIAGNOSIS OF FETAL ABNORMALITIES USING NUCLEATED RED BLOOD CELLS - The present invention relates to methods for diagnosing a condition in a fetus by enriching and enumerating circulating red blood cells with the possible combination of results from maternal serum marker screens. | 07-16-2009 |
| 20090197299 | Method for Assessing Airborn Microorganisms - The present invention relates to an environmental sampling method for assessing airborne microorganisms. The method comprises retaining organisms suspended in air to a polymeric pad via gravitational settling or forced impact, dissolving the polymeric pad in a buffered salt solution, and determining the number and kind of microorganisms in the buffered salt solution. Neither the polymeric pad nor the buffered salt solution inhibits the growth of the microorganisms to be determined. | 08-06-2009 |
| 20090221025 | SENSING DEVICE AND METHOD FOR RAPIDLY DETERMINING CONCENTRATIONS OF MICROBIAL ORGANISMS USING INTERFACIAL PHOTO-VOLTAGES - A system for detecting a wide range of microbial organisms, including virus, and determining concentrations in near real-time to determine titer, without the requirement to grow micro-organisms includes an electrometer configured to measure photo-induced interfacial voltages and an electrode assembly with a substrate and at least one electrode on a surface of the substrate electrically coupled to the electrometer. An attachment factor is applied to an exposed surface of each electrode. The attachment factor is effective for interaction with the microbial organism. A transparent vessel for containing the electrolytic solution is provided. The microbial organism may be contained in the electrolytic solution or applied to the coated electrode before being submerged in the electrolytic solution. A light source is configured to controllably produce a flash of activating light directed through the transparent vessel at the electrode causing a sensible photo-induce interfacial voltage indicative of the microbial organism and titer. A corresponding method includes steps of preparing the electrode surfaces with an attachment factor and exposing the submerged electrode surfaces to a flash of activating light to induce interfacial voltages indicative of a determined microbial agent and titer. | 09-03-2009 |
| 20090233329 | MICROFLUIDIC CHAMBER ASSEMBLY FOR MASTITIS ASSAY - The present invention relates to a device and method for the detection of mastitis or other disease from a body fluid of a mammal for example from cow's milk. The device and method relates to a wedge microfluidic chamber for using a minimal amount of fluid and being able to use the device to observe leukocytes in a mono-layer for the purpose of disease detection, cell counts or the like. | 09-17-2009 |
| 20090233330 | Method and apparatus for measuring changes in cell volume - A method and apparatus for measuring changes in cell volume generally includes introducing cells into a chamber having a volume between 2 and 100 times the volume of the introduced cell. A first electrically conductive extracellular fluid is introduced into the chamber and a current is applied. The voltage induced by said current flow is measured. The first fluid is exchanged with a second electrically conductive extracellular fluid and a current is applied. The voltage induced by said current flow is measured. The first induced voltage result and the second induced voltage result are used in conjunction with known voltages induced by such current flows to monitor changes in the volume corresponding to fluid flow between the cell and an extracellular fluid. | 09-17-2009 |
| 20090246823 | EVALUATION METHODOLOGY OF THE PROTECTION CHARACTERISTICS OF PERSONAL PROTECTIVE EQUIPMENTS AGAINST BIOLOGICAL AGENTS - New testing methodology for the evaluation of the protection of the Personal Protective Equipments (PPE) for the respiratory tract against biological agents, characterized in that different machineries are used to reproduce the usage of the PPE, simulating a breathing through the Sheffield's head and self-respirator. The apparatus consists of: a) a viral and/or bacterial aerosol generator b) a test chamber containing the Sheffield's head c) a respirator simulating breathing and adjusting the inspiration and expiration frequency d) a suction system delivering the samples of air withdrawn in different points to the bubblers to determine the viral and/or bacterial concentrations. | 10-01-2009 |
| 20100047854 | METHOD FOR DETECTION OF HUMAN PRECURSOR T CELL AND PRECURSOR B CELL - A method for detection of a precursor T cell or precursor B cell, a method for evaluation of the property of a hematopoietic precursor cell in a source for transplantation, and a kit for use in the evaluation are disclosed. A precursor T cell or precursor B cell can be detected by co-cultivating a stromal cell line with a monocyte. By using the detection method, a precursor T cell or precursor B cell can be quantified and can also evaluate the property of a hematopoietic precursor cell in a source for transplantation. | 02-25-2010 |
| 20100047855 | METHOD FOR DETERMINING THE QUANTITY OF MICROBIOLOGICAL OBJECTS DURING CULTIVATION THEREOF - A method for determining the quantity of microbiological objects during the cultivation thereof comprises determining a modification of studied microorganism cells' population, measuring the morphological composition thereof by determining the cells' size distribution in a liquid medium according to intensity changes of the light dispersed thereby. The method comprises probing a liquid flow by monochromatic coherent light, recording signals relating to the interaction between the probing radiation and the cells by measuring amplitudes and durations of impulses of the light dispersed by medium particles, plotting functions, according to measurement results, as two-dimensional distributions of normalized values of the amplitudes and durations in the form of statistic parameters of the dispersed light intensity. According to the functions, the size distribution of the cells and decomposition products of the liquid nutrient medium during the cultivation thereof are obtained. The invention can be applied for medical diagnostics and for control of biotechnological processes. | 02-25-2010 |
| 20100047856 | Bacteria analyzer, bacteria analyzing method and computer program product - The present invention is to present a bacteria analyzer comprising: a detector comprising: a light source for irradiating light on a measurement sample prepared from a specimen and a reagent; and a light receiving unit for receiving light generated by irradiating the light on the measurement sample from the light source; a scattergram data acquirer for acquiring scattergram data for generating a scattergram having information related to size of the bacteria contained in the specimen and fluorescence information generated by the bacteria as parameters; a bacteria number acquirer for acquiring number of bacteria contained in a plurality of regions on the scattergram for each region; and a form determiner for determining a form of the bacteria contained in the specimen. | 02-25-2010 |
| 20100047857 | MULTIPLE ANALYTE IMMUNOASSAY - Methods for measuring the amount of two or more analytes of interest in a fluid sample, and kits useful in the methods, are disclosed. The methods involve determining a ratio of a detected amount of a single analyte of interest, to the sum of a detected amount of each of the analytes of interest plus a detected amount of a control, wherein the amount of each analyte of interest is directly or inversely related to the ratio for each analyte of interest. | 02-25-2010 |
| 20100047858 | HIGH THROUGHPUT SYSTEM FOR CFU ASSAY BY THE USE OF HIGH RESOLUTION DIGITAL IMAGING, DIFFERENTIAL STAINING AND AUTOMATED LABORATORY SYSTEM - Disclosed herein is a high throughput system for the objective, standardized determination of colony forming units in populations of hematopoietic cells and indication of successful engraftment. Further disclosed is a laboratory information management system that provides electronic storage of images and data associated with cord blood units. | 02-25-2010 |
| 20100055735 | EX VIVO FLOW CYTOMETRY METHOD AND DEVICE - The invention relates to a method for diagnosing a disease state mediated by pathogenic cells. The method comprises the steps of combining with an ex vivo patient sample a composition comprising a conjugate or complex of the general formula | 03-04-2010 |
| 20100075369 | Analyzing Reticulocytes - Methods, systems, and computer program products for the analysis of particle analyzer data are disclosed. One embodiment is a method of analyzing immature reticulocytes in a blood sample, including the steps of: preprocessing the blood sample; measuring the blood sample by a detection including a reticulocyte-maturity measurement and a light scatter measurement; analyzing blood cell distribution patterns to identify a set of reticulocyte events; differentiating immature reticulocytes from mature reticulocytes using the reticulocyte-maturity measurement and the light scatter measurement; and reporting immature reticulocytes. The immature reticulocyte fraction may be one aspect that is reported. Also another method is disclosed, having the steps of: measuring the blood sample by a detection comprising an axial light loss measurement; identifying a hard-to-ghost cell population based on the axial light loss measurement; filtering-out the hard-to-ghost cell population; and analyzing the event data to identify blood cell distribution patterns. | 03-25-2010 |
| 20100075370 | METHOD FOR DETERMINATION OF CELL VIABILITY BY USING FLOW CYTOMETRY WITH FIXED VOLUME ACQUISITION - The present invention provides a method for comparing the content of a specific cell subpopulation in cell suspension samples by determining the count of cells of said subpopulation per unit volume in each sample, using a flow cytometer with fixed volume acquisition, but without the use of an internal or external standard. Thus, the effect of a test compound on cell viability can be determined, especially the cytotoxic or cytostatic effect of anticancer drugs. The method is a useful tool for predicting the biological activity of cytotoxic or cytostatic compounds on cells of the same kind as the cells in the sample. Furthermore, a kit for performing the method is provided. | 03-25-2010 |
| 20100075371 | METHOD AND APPARATUS FOR DETECTING AND QUANTIFYING BACTERIAL SPORES ON A SURFACE - A method and an apparatus for detecting and quantifying bacterial spores on a surface. In accordance with the method: a matrix including lanthanide ions is provided on the surface containing the bacterial spores; functionalized aromatic molecules are released from the bacterial spores on the surface; a complex of the lanthanide ion and the aromatic molecule is formed on the surface; the complex of the lanthanide ion and the aromatic molecule is excited to generate a characteristic luminescence of the complex on the surface; and the bacterial spores exhibiting the luminescence of the complex on the surface are detected and quantified. | 03-25-2010 |
| 20100112630 | METHODS FOR MEASURING MICROBIOLOGICAL CONTENT IN AQUEOUS MEDIA - A process for measuring total microbiological content in an aqueous medium includes adding a fluorescent dye to the aqueous medium, measuring the fluorescent signal in the aqueous medium to obtain a baseline fluorescent signal, releasing intracellular content of the microbiological matter into the aqueous medium by lysing the microbiological matter, measuring the fluorescent signal in the aqueous medium with the released intracellular content of the microbiological matter to obtain a second fluorescent signal, subtracting the baseline signal from the second fluorescent signal to obtain a net fluorescent signal and equating the net fluorescent signal with a microbiological content. Methods for measuring biofilm and adjusting for background noise are also provided. | 05-06-2010 |
| 20100112631 | Chip Having Microchannel For Counting Specific Micro Particles Among Floating Micro Particle Mixture By Optical Means And A Method For Counting Micro Particles Using The Same - A microchannel chip for counting floating microparticles with an optical method is provided. The depth of the microchannel chip is made to be a depth of field such that a clear and sharp optical image of microparticles can be obtained, without necessarily diluting the microparticle mixture inside the microchannel. Consequently, a microparticle counting instrument can easily count microparticles shown on an optical image of the microchannel. Particularly, it enables to count more easily the number of CD3 cells CD4 cells or CD8 cells in white blood cells being stained, without lysing or diluting red blood cells. Therefore, the microchannel chip can advantageously used for counting the number of CD3 cells, CD4 cells or CD8 cells in blood of an AIDS patient to monitor the progression of AIDS. | 05-06-2010 |
| 20100136611 | METHOD OF DETECTING BACTERIAL CONTAMINATION USING DYNAMIC LIGHT SCATTERING - Methods of detecting bacterial contamination in a platelet concentrate are performed using a dynamic light scattering (DLS) instrument and a sample holder. A sample of platelet concentrate can be held vertically or horizontally in a capillary in the sample holder. Alternatively, novel platelet storage bags modified to include an optically translucent window can be held within another variant of the sample holder. Still alternatively, platelet storage bags having one or more tubes detachably appended to the bag can be used. A sample is drawn off into an appended tube for placement directly into the sample holder. This method provides a number of related, non-invasive techniques for detecting whether bacteria has contaminated a platelet concentrate. Contamination indicators include a population of particles different from platelets, microparticles or proteins, bad-quality platelets, i.e. low DLS score, and very high or very low scattering intensity. | 06-03-2010 |
| 20100151510 | SUBSTRATE FOR CELL CULTURE, PRODUCING METHOD THEREOF AND SCREENING METHOD FOR CELL CULTURE CONDITIONS UTILIZING THE SAME - A cell culture substrate having at least one area for culturing a cell on a substrate, characterized in that the culturing area comprises an area releasably holding a biologically active substance having a biological activity to the cell and an area for immobilizing a biologically active substance having a biological activity to the cell. | 06-17-2010 |
| 20100159506 | METHODS AND SYSTEMS FOR GENETIC ANALYSIS OF FETAL NUCLEATED RED BLOOD CELLS - Methods for determining genetic status of a fetus from a sample of maternal blood comprise enriching nucleated red blood cells in the sample, including both fetal and maternal nucleated red blood cells. The nucleated red blood cells are then differentiated from all other blood cells in the enriched sample, and the nucleated red blood cells genetically screened to determine the genetic status. The nucleated red blood cells may be differentiated by immobilizing all enriched blood cells on a solid phase and locating the nucleated red blood cells for interrogation. Optionally, the nucleated red blood cells may be sorted and separated from other enriched blood cells in a liquid phase. | 06-24-2010 |
| 20100184124 | Detection of Bacteria or Somatic Cells in Organic Samples by ATP Based Luminescence - A method of testing an organic sample to provide an indication of the level of bacteria and/or number of somatic cells present in the sample, the method including collecting the sample and contacting the sample with a reagent which reacts with ATP to produce light emissions, and subsequently, at the beginning (O) of a measurement period (A, B), contacting the sample with an extractant in the presence of the reagent, the extractant rupturing cells present in the sample to release cell-bound ATP to react with the reagent to produce light emissions, and immediately after contacting the sample with the extractant, detecting the light emissions with a light detecting device ( | 07-22-2010 |
| 20100190204 | Detection and Identification of Microorganisms on Transparent Permeable Membranes - This invention describes rapid detection and identification of colonies or micro-colonies of microorganisms after regular or short (several hours) growth periods on light pellucid, molecule-permeable membranes installed on solid nutrient media. Colonies or micro-colonies appearing on a membrane can be easily transferred from a growth plate to another media such as, pure agar or paper filled with indicator substances or substrates. Filterable and non-filterable samples can be analyzed by this method. A multitude of different methods of detection and identification can be realized using this invention in a micro-colony format: detection and enumeration of all live cells or specific live cells; detection and simultaneous identification of antibiotic-resistant microorganisms; different immunological methods of detection; detection and enumeration using machine analysis such as automated image identifiers. | 07-29-2010 |
| 20100221772 | AUTOMATED CELL DENSITY ADJUSTMENT METHOD FOR PRODUCING AN ANALYSIS PLATE - This method includes the following steps of:
| 09-02-2010 |
| 20100248300 | BLOOD ANALYZER, BLOOD ANALYZING METHOD, AND COMPUTER PROGRAM PRODUCT - A blood analyzer comprising: a specimen preparation section for preparing a measurement specimen that is used to measure a white blood cell count among CBC measurement items; a measurement section for obtaining optical information from blood cells contained in the measurement specimen; and a controller carrying out operations comprising: classifying blood cells contained in the measurement specimen into at least white blood cells and blood cells suspected to be abnormal blood cells based on the optical information; obtaining distribution data of the white blood cells and distribution data of the blood cells suspected to be abnormal blood cells; counting the white blood cells based on the distribution data of the white blood cells; and determining presence or absence of the abnormal blood cells in the blood sample, based on the distribution data of the blood cells suspected to be abnormal blood cells. A blood analyzing method and a computer program product are also disclosed. | 09-30-2010 |
| 20100261225 | APPARATUS AND METHOD FOR ANALYZING BACTERIA - An apparatus for analyzing bacteria is described that includes an analytic sample preparation section for preparing an analytic sample by treating a specimen so as to generate a morphological difference between Gram-negative bacteria and Gram-positive bacteria, a detector for detecting optical information from each particle contained in the analytic sample and an analyzing section for detecting Gram-positive bacteria contained on the basis of the detected optical information. A method for analyzing bacteria is also described. | 10-14-2010 |
| 20100267080 | REAGENT FOR BLOOD ANALYSIS AND METHOD OF USE THEREOF - The present disclosure provides a reagent for blood analysis which may include: (1) a compound having the general formula I as a fluorescent dye, wherein n, X, R | 10-21-2010 |
| 20100273209 | CO2 OPTICAL SENSOR FOR DETECTION AND ENUMERATION OF MICROORGANISMS - A new device and method for detecting the presence of living microorganisms in test samples are described. The device includes a container having at least one section transparent to light with an incubation zone defined in the container, the incubation zone containing growth media in which the sample is cultured. A detection zone containing a matrix composed of a polymeric material which is substantially transparent to light, and at least one indicator reagent sensitive to carbon dioxide gas generated by the microorganisms in the incubation zone is located in the transparent section of the matrix. The matrix is configured to facilitate penetration of external light aimed at the transparent section of the container and interaction of the external light with the indicator reagent to yield interactive light that escapes through the transparent section of the container, said interactive light is being indicative of the presence and/or concentration of the microorganisms. | 10-28-2010 |
| 20100285523 | SYSTEM AND METHOD FOR ANALYZING FLUIDS - A spectrometric sensor for measuring a spectra value of a flowing fluid. The spectrometric sensor comprises a light source for emitting a first light flux toward a sample of the flowing fluid, a light detector for measuring a first intensity of a reflection of the first light flux from the flowing fluid and a second intensity of a second light flux received via the flowing fluid, and a control unit configured for generating at least one spectra value according to at least one of the first and second intensities. | 11-11-2010 |
| 20100311109 | NON-CONTACT METHOD FOR QUANTIFYING CHANGES IN THE DYNAMICS OF MICROBIAL POPULATIONS - A method for quantifying an amount of a viable microorganism includes subjecting a fluid sample suspected of containing a viable microorganism to a temperature change, and correlating the temperature history of the fluid sample to the amount of the viable microorganism contained in the fluid sample. The method may include the steps of bringing, the fluid sample to a first temperature, and transferring the fluid sample to a second temperature that is different than the first temperature. After the step of transferring, next is the step of measuring a temperature change in the fluid sample over a predetermined period of time. The temperature change may then be correlated to the amount of the viable microorganism contained in the fluid sample. The method finds use in a variety of applications, including evaluation of compositions or compounds potentially having microbicidal, microbiostatic, or growth enhancing properties. | 12-09-2010 |
| 20100323393 | Ordered Assembly of Membrane Proteins During Differentiation of Erythroblasts - Disclosed herein are methods for the isolation, identification, and quantification of red blood cells and red blood cell precursors at different developmental stages. Also disclosed are methods for monitoring ex vivo proliferation and differentiation of red blood cells and red blood cell progenitors. | 12-23-2010 |
| 20100323394 | INTEGRATED FILTRATION AND DETECTION DEVICE - An integrated filtration and detection device for collecting and detecting the growth of microorganisms in a specimen includes a container defining a chamber therein. The container has an inlet and an outlet in fluid communication with the chamber. A filter is mounted in the chamber between the inlet and the outlet. A sensor is mounted in the chamber. The sensor is operative to exhibit a change in a measurable property thereof upon exposure to changes in the chamber due to microbial growth. | 12-23-2010 |
| 20110008825 | System for Conducting the Identification of Bacteria in Urine - A system for conducting the identification and quantification of micro-organisms, e.g., bacteria in urine samples which includes: 1) several disposable cartridges for holding four disposable components including a centrifuge tube, a pipette tip having a 1 ml volume, a second pipette tip having a 0.5 ml volume, and an optical cup or cuvette; 2) a sample processor for receiving the disposable cartridges and processing the urine samples including transferring the processed urine sample to the optical cups; and 3) an optical analyzer for receiving the disposable cartridges and configured to analyze the type and quantity of micro-organisms in the urine sample. The disposable cartridges with their components including the optical cups or cuvettes are used in the sample processor, and the optical cups or cuvettes containing the processed urine samples are used in the optical analyzer for identifying and quantifying the type of micro-organism existing in the processed urine samples. | 01-13-2011 |
| 20110014646 | SAMPLE PREPARATION APPARATUS AND SAMPLE PREPARATION METHOD, AND CELL ANALYZER AND CELL ANALYSIS METHOD - A sample preparation apparatus comprising: a detector for detecting a predetermined cell included in a biological sample; a sample preparation section for preparing a measurement sample from the biological sample and a predetermined reagent; and a controller configured to generate, based on a detection result by the detector, concentration information reflecting a concentration of the predetermined cell in the biological sample and to control, based on the concentration information, the amount of the biological sample supplied to the sample preparation section. | 01-20-2011 |
| 20110020861 | METHOD FOR THE REAL-TIME DETECTION OF MICROORGANISMS IN A LIQUID CULTURE MEDIUM BY AGGLUTINATION - A method for detecting at least one microorganism that may be present in a sample, comprising the steps of: | 01-27-2011 |
| 20110027824 | Method And Apparatus For Analyzing Body Fluids - A system and method for analyzing a specimen containing particles that can be difficult to differentiate. The system and method determines a first collective count of a selected group of particles in the specimen, treats at least a portion of the specimen to alter a subgroup of the selected group of particles, determines a second collective count of any of the selected group of particles in the treated portion of the specimen, and subtracts the second collective count from the first collective count to determine a differentiation count for the subgroup of particles altered by the treating of the specimen. The system and method is described with the example of determining concentrations of red and white blood cells in a specimen (e.g. spinal fluid), using auto-particle recognition techniques, without attempting to distinguish and count red versus white blood cells co-existing in the same specimen portion. | 02-03-2011 |
| 20110027825 | HIGH RESOLUTION CLASSIFICATION - The present invention relates to a method of determining pulse height distribution by using an apparatus comprising: an analogue to digital pulses height categorisation unit comparing the pulse to analogue threshold voltages and counting each event within each pulse height category using a micro controller. The method may comprise the steps of i) selecting a first set of threshold voltages, ii) performing a first measurement using the first set of threshold voltages, iii) selecting a new set of threshold voltages different from the first set of threshold voltages, iv) performing a new measurement using the new set of threshold voltages, v) determining cell size distribution based on the first measurement and the new measurement. The present invention further relates to an apparatus comprising an analogue to digital pulses height categorisation unit comparing the pulse to analogue threshold voltages, a micro controller configured for counting each event within each pulse height category, the micro controller further configured for i) selecting a first set of threshold voltages, ii) performing a first measurement using the first set of threshold voltages, iii) selecting a new set of threshold voltages different from the first set of threshold voltages, iv) performing a new measurement using the new set of threshold voltages, v) determining cell size distribution based on the first measurement and the new measurement. | 02-03-2011 |
| 20110027826 | LEUKOCYTE ANALYSIS METHOD AND ANALYSIS REAGENT FOR USE IN THE METHOD - The present invention provides a method for analyzing leukocytes, by which the leukocytes can be classified and measured stably with high accuracy even when a dilution ratio of a sample containing the leukocytes is low or a flow velocity during the analysis is slow, and an analysis reagent used for the analysis method. The analysis method of the present invention includes the steps of mixing a sample containing leukocytes and erythrocytes and an analysis reagent containing a surfactant that reacts with leukocytes; and measuring the leukocytes by passing a mixed solution of the sample and the analysis reagent through a fine through-hole, measuring a signal detected when the mixed solution passes through the fine through-hole, and classifying and counting the leukocytes in the sample. The analysis reagent further contains a nonionic surfactant, and the nonionic surfactant has a sugar residue as a hydrophilic region and an aliphatic chain as a hydrophobic region. | 02-03-2011 |
| 20110039297 | Method and Device for Controlling the Fermentation Process - A method and device for controlling a fermenting process, wherein the method comprises collecting samples of biological cells from a fermenting tank, obtaining the state information of the current biological cells based on the collected samples, comparing the state information of the current biological cells with preset target status information to obtain the difference between the state information of the current biological cells and preset target state information, and controlling the feed rate of nutritional solution into the fermenting tank. Real time control of biological fermenting process is thus accomplished based on the state information of the biological cells during fermentation, and the consistency during fermentation is improved. Complicated mathematical modeling and man-made data analysis are not required for the method and device. Real time control of biological fermenting process is accomplished based on a feedback loop with simply calculation, and control delay is not created, and automatic control of cells statement in fermentation is achieved. | 02-17-2011 |
| 20110045527 | MEASUREMENT OF PROTEIN USING INCLUSION BODY DRY WEIGHT - The present invention is directed to improved methods for efficiently producing recombinant proteins. More specifically, the invention relates to a process for calculating the protein in inclusion bodies before the refolding step in large scale recombinant protein production, thereby improving the efficiency of the refolding step and overall yield and quality of the sample protein. | 02-24-2011 |
| 20110059483 | Molecular Probe for Imaging of Pancreatic Islets and Use of the Same - To provide a molecular probe for imaging of pancreatic islets. A molecular probe for use in imaging of pancreatic islets is provided. The molecular probe includes any one of the following polypeptides; polypeptides represented by the following formulae (1), (5), and (9); and polypeptides having homology with the foregoing polypeptides; | 03-10-2011 |
| 20110065145 | WATER MAMAGEMENT - A method for detecting and enumerating viable microorganisms in a sample suspected of containing said microorganisms (1) contacting said microorganisms of said sample with at least one repair compound and a growth medium, and (2) incubating the product of steps (1), and (3) detecting and quantifying said viable microorganisms, in which the microorganisms are of the species | 03-17-2011 |
| 20110070606 | SYSTEMS AND METHODS FOR ANALYZING BODY FLUIDS - Systems and methods analyzing body fluids contain cells including blood, bone marrow, urine, vaginal tissue, epithelial tissue, tumors, semen, and spittle are disclosed. The systems and methods utilize an improved technique for applying a monolayer of cells to a slide and generating a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and method for utilizing multi-color microscopy for improving the quality of images captured by a light receiving device. | 03-24-2011 |
| 20110076717 | CULTURE MEDIUM CONTAINING A SPORE GERMINATION INHIBITING OR DELAYING COMPOUND - A medium for the culture and detection of target microorganisms, having at least one natural or synthetic specific substrate configured to detect at least one enzyme activity or metabolic activity of the target microorganisms and at least one compound that inhibits or delays the germination of spores of microorganisms, other than the target microorganisms, that are capable of interfering with the culture and detection of the target microorganisms. | 03-31-2011 |
| 20110091933 | METHOD OF TREATING MICROORGANISM - A method of treating microorganisms which can solve conventional problems in a treatment of counting the number of microorganisms, a proliferation treatment of microorganisms and a purification treatment of microorganisms is provided. A method of treating microorganisms in which any one of the intended treatments of a treatment of counting the number of microorganisms, a proliferation treatment of microorganisms and a purification treatment of microorganisms is carried out, the method including, before carrying out an intended treatment step, a pretreatment step of packing a predetermined amount of a sample solution containing the microorganisms in a closed container 22 and subjecting the closed container to high speed oscillating motion. | 04-21-2011 |
| 20110104744 | CELL ANALYZING APPARATUS AND CELL ANALYZING METHOD - A cell analyzing apparatus, comprising: a parameter obtaining section for obtaining a characteristic parameter from a cell in a measurement sample; an imaging section for capturing an image of the cell in the measurement sample; an analyzing section for counting a cell in which the characteristic parameter meets a predetermined requirement among the cells in the measurement sample as a counting target and generating output data based on a counting result; a display section for displaying an image of the cell meeting the predetermined requirement and the output data; and an input section for receiving an instruction to specify the image displayed on the display section, wherein the analyzing section excludes a cell relevant to the specified image from the counting target and regenerates the output data is disclosed. A cell analyzing method is also disclosed. | 05-05-2011 |
| 20110104745 | METHOD OF EXPRESSING RECOMBINANT PROTEIN IN CHO CELLS - Method for enhancing the transfection rate of a mammalian expression vector in CHO cells. | 05-05-2011 |
| 20110104746 | Diluting Solution For Suspension For Use In The Measurement Of Number Of Living Microbial Cells Contained In Sample, And Method For Measurement Of Number Of Living Microbial Cells - A dilution buffer for suspension and an enumeration method, which, for the measurement of the viable cell count of a microorganism contained within a sample, and particularly a powdered product such as a milk powder, yield more accurate measurement values than have conventionally been obtainable. A dilution buffer for suspension, used for preparing a suspension buffer of a sample when conducting a measurement of the viable cell count of a microorganism contained within the sample, wherein the dilution buffer contains polysorbates at a concentration of not less than 0.5%. Also, an enumeration method for a microorganism contained within a sample, the method including: preparing a suspension buffer of the sample using a dilution buffer for suspension containing polysorbates at a concentration of not less than 0.5%, and measuring the viable cell count of the microorganism contained within the suspension buffer. | 05-05-2011 |
| 20110136165 | DETECTING OBJECTS - The invention provides apparatus and methods for detecting objects in samples. The sample is held in the transmission path of light from a light source to a detector, whereby light from the light source interacts with objects in the sample. The patterns of light incident on the detector subsequent to its interaction with the objects are directly used to determine the presence of objects in the sample. | 06-09-2011 |
| 20110151502 | METHOD FOR PERFORMING A BLOOD COUNT AND DETERMINING THE MORPHOLOGY OF A BLOOD SMEAR - A method for counting blood cells in a sample of whole blood. The method comprises the steps of:
| 06-23-2011 |
| 20110151503 | DEVICE AND METHOD FOR BACTERIOLOGICAL TESTING ON PLASMA - Apparatus for making a bacteriological test on plasma, comprising a sedimentation unit for a blood sample contained in a first container to separate the corpuscular part of the sample, which sediments on the bottom of the first container, from the liquid part or plasma, pick-up and inoculum means to pick up a portion of the surnatant, and to inoculate the portion in a culture ground inside a second container allowing a bacterial growth, optical measurement means, to effect measurements of the culture ground in order to determine the presence of bacteria and microorganisms, and processing means comprising a data bank, to collect measurement data, to construct a curve that represents the intensity of the radiation diverted by the culture ground in the measurement with respect to time, whose parameters are compared with reference values in order to determine typical analysis parameters, said values being characteristic for each bacterial species. Disclosed is further a corresponding method. | 06-23-2011 |
| 20110171684 | INSPECTION METHOD OF AIRBORNE FLOATING BACTERIA AND ITS APPARATUS - Inspection arrangements of airborne floating bacteria for performing a biological light emission reaction by using an ATP light emission reagent on the basis of the ATP derived from viable cells in captured airborne floating bacteria in the air contained in the inspection sample, measuring the light emission quantity due to the biological light emission reaction, determining the ATP quantity contained in the inspection sample, and counting the number of cells in the airborne floating bacteria, the light emission quantity of the ATP light emission reagent is measured, and a measured value of light emission quantity of the ATP light emission reagent and a predetermined theoretical value corresponding to the measured value are compared, and thereby the reliability of the ATP light emission reagent to be used and the inspection results is evaluated at the same time. | 07-14-2011 |
| 20110177548 | Fluorescent microscope slide - An apparatus and method for increasing the efficiency of finding a field of focus, and for increasing the accuracy of field of view in reading slides with fluorescent microscopy technology, including tuberculosis slides. | 07-21-2011 |
| 20110177549 | METHOD, KIT AND SYSTEM FOR CULTURABLE CELL COUNT - The present invention provides a method, kit and system for determining a quantitative value indicative of the number of culturable microbial cells, such as | 07-21-2011 |
| 20110183371 | MICROBE-COLLECTING CARRIER CARTRIDGE, CARRIER TREATING APPARATUS, AND METHOD OF MEASURING MICROBES - A disposable microbe-collecting carrier cartridge and a carrier treating apparatus which efficiently convert microbes collected on a carrier into a solution and prevent variations in the amount of retrieval of microbes upon concentration on a filter are provided. A cartridge formed of an upper lid having a plurality of through holes, a container in an inverted-cone shape having both of a liquid storage sink unit having a filter at the bottom and an air suction path, and a support base for setting up a thermoplastic carrier, such as gelatin, and a carrier treating apparatus formed of a dispensing nozzle for liquid supply, a liquid supply mechanism, a liquid heating mechanism, and a suction pump for filtration are prepared. Warm water is supplied onto the filter set up on a bottom surface of the inverted-cone-shaped container and is stored in the liquid storage sink, thereby causing the carrier to make contact with the warm water. The contact with the warm water causes the carrier to be solated, and mixed and diluted with the warm water. Then, the suction pump of the filtration device is activated for suction and filtration of the mixed liquid through the filter. By using the filter having a shape not allowing microbes to pass through and having a gap, only microbes are captured on the filter after suction and filtration. Polymers and dust to be a background upon an ATP measurement pass through the filter by filtration to be cleanly eliminated. | 07-28-2011 |
| 20110244511 | METHODS AND ARTICLES FOR DETECTING HEMOLYTIC MICROORGANISMS - A thin film culture plate device useful for detecting hemolysin-producing microorganisms is included. The device can further include selective agents and/or indicators to differentiate groups or species microorganisms. Methods of use include detecting or enumerating hemolysin-producing microorganisms. | 10-06-2011 |
| 20110250633 | Device and Method for Automatically Analyzing Micro Organisms in a Sample - Device for automatically analyzing micro organisms in an aqueous sample using filter cytometry comprising at least one filter holder and processing modules, wherein the filter holder is arranged to contain a filter for receiving the sample, wherein the processing modules comprise sample application means for applying the sample to the filter in the holder and imaging means for imaging the micro organisms on the filter, wherein the device furthermore comprises displacement means for automatically moving the filter holder between the processing modules and wherein each of the modules and the filter holder are arranged to removable connect for interaction. | 10-13-2011 |
| 20110262961 | Diagnosis, Prevention and Treatment of Disorders Characterized by Undesirable Cell Proliferation - The present invention relates to compositions and methods for the reduction of atherosclerotic plaques and the decrease in the level of total serum cholesterol, triglycerides, serum LDL cholesterol, and serum HDL cholesterol. The present invention also relates to methods for the diagnosis, prevention and treatment of atherosclerosis and mycoplasma associated diseases. | 10-27-2011 |
| 20110275115 | METHOD TO DETECT ENDOTHELIAL CELL MASSIVE CALCIUM ACCUMULATION DEATH - Stains that are specific for calcium ion are used to assess and predict the effects of various treatments on the viability of cell types contained in a sample, wherein said stain detects endothelial cells that have massive calcium accumulation death. | 11-10-2011 |
| 20110312022 | METHOD FOR DETERMINING PERCENT HYBRIDITY IN MODIFIED HYBRID CROPS - Methods of introducing sentinel traits into foundation seed are described. The frequency of occurrence of the sentinel trait in the hybrid seed product relative to the frequency of the sentinel trait in a pollinator seed product are used to determine the percentage of hybridity in modified hybrid varieties. | 12-22-2011 |
| 20110318777 | DEVICE FOR METERING CELLS, METHOD FOR METERING CELLS AND ALSO USE OF THE DEVICE - The invention relates to a device for metering cells and also to a method for metering cells. Furthermore, the invention relates to the use of the device for metering cells. | 12-29-2011 |
| 20120003687 | METHODS FOR COUNTING CELLS - The invention features methods of quantifying cells in a sample by lysing the cells followed by the measurement of at least one intracellular component. Methods of the invention are especially useful for quantifying small numbers of cells, e.g., over a large surface area or volume compared to the cell size. In a preferred embodiment, methods of the invention are performed using a microfluidic device. | 01-05-2012 |
| 20120028297 | ENVIRONMENTAL SAMPLING ARTICLES AND METHODS - The present invention refers to articles for collecting samples from a surface, articles for microbiological analyses of said samples, and methods of use of said articles. The articles include sample collectors, sample housings with optional barrier layers, and sample-ready reagent strips comprising hydrophilic agents to grow and detect microorganisms. The disclosure includes methods to collect, detect, and quantify microorganisms in a surface sample. | 02-02-2012 |
| 20120064567 | ACTIVE MICRO SIEVE AND METHODS FOR BIOLOGICAL APPLICATIONS - An active sieve device for the isolation and characterization of bio-analytes is provided, comprising a substrate for supporting the bio-analytes. The substrate comprises a plurality of interconnections and a plurality of regions, each region comprising a hole and at least one electrode embedded in or located on the substrate and electrically associated with the hole. Each region further comprises at least one transistor integrated in the substrate and operably connected to the at least one electrode and to at least one of the plurality of interconnections. | 03-15-2012 |
| 20120094327 | DETECTION OF ACID-PRODUCING BACTERIA - The disclosure provides culture devices and methods useful for detecting acid-producing bacteria in a sample. The devices include a nutrient medium and a pH indicator to detect and differentiate acid-producing microorganisms, such as lactic acid bacteria. Methods of use include detecting or enumerating acid-producing microorganisms. The methods further provide for the detection of gas-producing acid-producing bacteria. | 04-19-2012 |
| 20120129211 | TITRATION OF DIFFERENTIATION MEDIUM COMPONENTS - Methods and composition for differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including screening of optimal differentiation conditions for a selected stem cell clone in a selected batch of culture medium and use of the determined optimal condition for differentiation into a specific cell lineage. | 05-24-2012 |
| 20120129212 | METHOD OF MONITORING ERYTHROPOIESIS - The invention relates to methods of monitoring erythropoiesis. In particular, the invention relates to methods of detecting nascent erythrocyte production in vivo as methods for identifying modulators of erythropoiesis. | 05-24-2012 |
| 20120149058 | PROCESS AND APPARATUS FOR TESTING SUBSTANCES FOR POTENTIAL CARCINOGENICITY - An apparatus ( | 06-14-2012 |
| 20120164684 | SAMPLE MEASURING APPARATUS AND SAMPLE MEASURING METHOD - A sample measuring apparatus of measuring a component in a biological sample, comprising: an input section for inputting a sample species of a biological sample; a measurement sample preparation section for preparing a measurement sample by mixing the biological sample with a reagent; a first measurement section; a second measurement section being different from the first measurement section; a measurement sample supply section for supplying the measurement sample prepared in the measurement sample preparation section to at least one of the first measurement section and second measurement section; and a control section for controlling the measurement sample supply section based on the inputted sample species. | 06-28-2012 |
| 20120171717 | METHOD OF SELECTING SAFE PLURIPOTENT STEM CELLS - Provided is a method of selecting highly safe pluripotent stem cells that do not exhibit differentiation resistance, comprising the steps of (1) inducing a pluripotent stem cell to differentiate, (2) culturing the cell under conditions for maintaining undifferentiated state, (3) detecting the generation of an undifferentiated cell by the cultivation, and comparing the finding with a control, and (4) selecting a pluripotent stem cell whose detected value is not more than a control generation value. | 07-05-2012 |
| 20120190060 | MICROFLUIDIC DEVICES AND METHODS FOR MALARIA DETECTION - A device for identifying infection by the malaria parasite includes a microfluidic device having an inlet and an outlet and a diagnostic channel interposed between the inlet and the outlet. The diagnostic channel includes a contact surface and a sample pump configured to pump a RBC-containing sample into the inlet. The contact surface may be at least one of hydrophilic and roughened. Malaria infected RBCs (miRBCs) interact with the contact surface and become immobilized thereon whereas non-infected RBCs continue to flow downstream in the diagnostic channel. | 07-26-2012 |
| 20120190061 | NOVEL STRATEGY TO REDUCE LACTIC ACID PRODUCTION AND CONTROL PH IN ANIMAL CELL CULTURE - The present disclosure provides a method for culturing cells in exogenous lactic acid. Certain aspects of the present disclosure include the production of recombinant proteins, such as antibodies and fragments thereof. Certain aspects of the present disclosure also relate to methods of controlling lactic acid production, pH stability and osmolality in cell culture. | 07-26-2012 |
| 20120231493 | APPARATUS FOR CHEMILUMINESCENT ASSAY AND DETECTION - An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member, and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit, and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less. | 09-13-2012 |
| 20120244571 | MEASUREMENT METHOD FOR VIABLE CELL COUNT, AND CULTURE MEDIUM - There are provided a method of identifying a microorganism belonging to | 09-27-2012 |
| 20120244572 | METHOD AND APPARATUS FOR ENHANCED DETECTION OF TOXIC AGENTS - A water quality analyzer for real-time detection according to the invention comprises a biased AC electro-osmosis (ACEO) cell for receiving a fluid to be analyzed having a plurality photosynthetic organisms therein, and concentrating the plurality photosynthetic organisms into at least one concentrated region. A photodetector is provided for obtaining a measured photosynthetic activity of the plurality of photosynthetic organisms in the concentrated region: wherein chemical, biological or radiological agents reduce a nominal photosynthetic activity of the photosynthetic organisms. An electronics package analyzes the measured photosynthetic activity to indicate a presence of the chemical, biological or radiological agents in the fluid. | 09-27-2012 |
| 20120244573 | SAMPLE MEASURING APPARATUS AND SAMPLE MEASURING METHOD - A method for analyzing blood cells in a whole blood sample obtained from a cat is provided. An electrical measurement result and an optical measurement result of the whole blood sample are acquired. The electrical measurement result is obtainable by electrically measuring blood cells in the whole blood sample and the optical measurement result is obtainable by optically measuring blood cells in the whole blood sample. On the basis of the electrical measurement result and the optical measurement result, volume of red blood cells in the whole blood sample is calculated. | 09-27-2012 |
| 20120244574 | ILLUMINATION APPARATUS AND METHODS FOR A BIOLOGICAL GROWTH PLATE SCANNER - Illumination apparatus for illuminating growth plates in a biological growth plate ( | 09-27-2012 |
| 20120252062 | Method for diagnosing and/or predicting the development of neurodegenerative diseases - The present invention relates to a method for diagnosing and/or predicting the development of neurodegenerative diseases; in the method, white blood cells are isolated and enriched or cultivated for forming colony-forming units, wherein CFU-M and other CFU are formed. Subsequently, the relative number of CFU-M formed in the previous cultivation(/enrichment step relative to the other CFUs formed are determined. | 10-04-2012 |
| 20120270261 | ANALYTICAL INSTRUMENT AND METHOD FOR EVALUATING MICROBIAL CONTAMINATION OF AN OBJECT - An instrument and method for ascertaining a viable aerobic microbe count at t | 10-25-2012 |
| 20120276582 | DISPOSABLE CASSETTE AND METHOD OF USE FOR BLOOD ANALYSIS ON BLOOD ANALYZER - A disposable cassette for blood analysis includes a housing having an upper panel and a sampling section having a filling inlet; at least one pair of chambers in a form of depression of the upper panel of the housing and sealed by a diaphragm; portions of the diaphragm over the chambers being flexible; and one or more channels adapted to interconnect the pair of chambers; one of the chambers containing a predetermined amount of a reagent for blood analysis; and a sample outlet disposed next to and connected to the chamber containing the reagent, the sample outlet including an outlet cavity recessed from the upper panel, a divider disposed therein, and a cover covering the outlet cavity; the sample outlet sealing the reagent to the chamber containing the reagent. Further disclosed is the method of using the disposable cassette for measurements of hematology parameters on a blood analyzer. | 11-01-2012 |
| 20120282650 | COMPOSITE MATERIAL - A composite material includes a body and a plurality of nano-scale probes. The body is made of a polymer. The plurality of nano-scale probes is embedded in the body. The nano-scale probes are substantially uniformly distributed in the polymer matrix. The nano-scale probes are nanowires, nano-particles or nanotubes | 11-08-2012 |
| 20120288889 | REAGENT FOR BLOOD CELL COUNTING AND BLOOD ANALYSIS METHOD - Disclosed is a novel reagent for blood cell counting and a novel blood analysis method, which enable blood cells such as leukocytes to be counted with high accuracy by dissociating platelet aggregates in capillary blood collected from a living body. The reagent for blood cell counting is used to dilute capillary blood collected from a living body to prepare a blood sample in order to count blood cells in the collected capillary blood using a particle analyzer, and is an aqueous solution containing a chloroquine salt. | 11-15-2012 |
| 20120288890 | MICROBIAL COUNTING CELL, MICROBE COUNTING DEVICE USING SAME, AND MICROBE COUNTING METHOD USING SAME - A microorganism counting cell of the present invention includes bottomed cylindrical vessel that includes upper surface opening and cylindrical retaining body disposed vertically on a bottom surface in vessel, a collecting element provided on a lower end portion of a rod-shaped cotton swab being inserted in retaining body from upper surface opening. In the microorganism counting cell, elution protrusion is provided on an interior side surface of retaining body, and elution groove that pierces retaining body from an inside to an outside is provided on a side surface of retaining body. | 11-15-2012 |
| 20120295300 | VIABILITY CELL COUNTING BY DIFFERENTIAL LIGHT ABSORPTION - Cell counts that distinguish between live and dead cells while providing an accurate count of the total of live and dead cells are obtained by the use of a vital stain in conjunction with illumination of the cell population and the taking of light images at different wavelengths, one which is not absorbed by the stain and one that is absorbed by the stain. Masking and inaccuracies in the counting of dead cells is thereby avoided. | 11-22-2012 |
| 20120295301 | FLOATING BACTERIA TRAPPING DEVICE, FLOATING BACTERIA COUNTING METHOD AND FLOATING BACTERIA COUNTING SYSTEM - A floating bacteria counting device capable of trapping floating bacteria in air continuously for a long time without decreasing trapping efficiency even if the time before a trapping carrier being replaced is prolonged and a floating bacteria counting method and a floating bacteria counting system using the floating bacteria trapping device are provided. A nozzle ( | 11-22-2012 |
| 20120295302 | MAGNETIC SEPARATION OF RARE CELLS - A magnetic separation system configured to separate with high qualitative and quantitative yield magnetized cells from cell mixtures, comprising at least one electromagnet structured to generate a magnetic field flux about a plurality of separation zones and sufficient to attract a majority of the magnetized cells in the mixture, and a pump to drive the cell mixture at a controlled flow rate through a tube disposed within the zones thereby separating a majority of the magnetized cells from the mixture. The system is particularly useful to retrieve rare cells from a fluid mixture of cells having low abundance of the rare cells relative to the rest of the cells while sustaining viability of the cells. | 11-22-2012 |
| 20120315666 | DETECTION APPARATUS AND METHOD FOR DETECTING AIRBORNE BIOLOGICAL PARTICLES - In a detection apparatus, an inlet and an outlet are opened and an air introducing mechanism is driven to introduce air to a case, and airborne particles are electrically attracted and held on a collecting jig | 12-13-2012 |
| 20120315667 | REAGENT, REAGENT KIT AND ANALYZING METHOD - A method for analyzing platelet is described. In the method, a measurement sample is prepared by mixing a sample and a dye for staining platelet. The dye is selected from the group consisting of Capri blue, Nile blue and brilliant cresyl blue. By irradiating cells in the measurement sample with light, scattered light and fluorescence emitted from the cells is measured. The platelet is detected on the basis of the scattered light and the fluorescence. | 12-13-2012 |
| 20120329087 | CELL PREPARATION CONTAINING MESENCHYMAL STEM CELLS, AND METHOD FOR PRODUCING SAME - A cell preparation containing mesenchymal stem cells whose immunosuppression ability is maintained is produced by means of a serum-free or low-serum culture. A method for producing a cell preparation containing mesenchymal stem cells, comprising the steps of: (A) proliferating mesenchymal stem cells in a serum-free medium “A” containing an FGF, a PDGF, a TGF-β, an HGF, an EGF, at least one phospholipid, and at least one fatty acid; and (B) screening mesenchymal stem cells whose immunosuppression ability is maintained or improved, from the mesenchymal stem cells thus proliferated in the step (A). | 12-27-2012 |
| 20130004989 | METHOD FOR ESTIMATING THE NUMBER OF MICROORGANISMS IN A SAMPLE AND DEVICE FOR IMPLEMENTING SAID METHOD - A method for counting microorganisms present in a biological sample in contact with a culture medium adapted to the growth of said microorganisms, characterized in that it includes the steps of:
| 01-03-2013 |
| 20130029374 | METHOD AND MEASURING POINT FOR MEASURING AT LEAST ONE PHYSICAL AND/OR CHEMICAL, PROCESS VARIABLE OF A MEASURED MEDIUM CONTAINED IN A SINGLE USE CONTAINER - A method, and a measuring point for performing the method, for measuring at least one physical and/or chemical, process variable of a medium contained in a single use container. A single use sensor is connected with the single use container. The single use sensor with the single use container is sterilized by radiation, especially beta or gamma radiation. An electronics unit is connected with the single use sensor, and the electronics unit is connected with at least one superordinated system via at least one interface. The electronics unit and/or the single use sensor are/is supplied with energy, and measurement data of the single use sensor are registered. The measurement data are processed by the electronics unit and/or the superordinated system, data, including measurement data, are transmitted to the superordinated system and/or received from the superordinated system, and, after termination of one or more measuring cycles, the electronics unit is released from the single use sensor. | 01-31-2013 |
| 20130059329 | APPARATUS AND METHOD FOR DISTRIBUTING LIQUID SAMPLES IN SMALL VOLUMES - Apparatus for dispensing liquids in small sample volumes, comprising a preformed rigid duct of impermeable material, with one inlet and one or more outlets, characterized in that the duct dimensions vary along it, forming communicating ducts, bifurcations and reservoirs; and a process for its manufacture. | 03-07-2013 |
| 20130089891 | APPARATUS FOR CHEMILUMINESCENT ASSAY AND DETECTION - An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member, and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit, and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less. | 04-11-2013 |
| 20130130310 | ABSORBENT PAPER AND USE THEREOF FOR BREAST CANCER DETECTION - Biological samples of mammary fluid or components thereof are obtained using a breast pump device coupled with an absorbent paper or membrane, optionally facilitated by administering oxytocin to the subject. The breast pump device stimulates expression of mammary fluid and provides for collection of diagnostic samples on the absorbent paper or membrane to evaluate breast disease, including cancer. The biological sample may include fluid containing one or more of cells or cellular components, proteins, glycoproteins, peptides, nucleotides or other desired constituents comprising a breast disease marker. Absorbent paper or membrane, and methods relating to the paper or membrane, and a breast pump device are also provided. | 05-23-2013 |
| 20130130311 | METHODS AND SYSTEMS FOR ASSESSING CLONALITY OF CELL CULTURES - Methods of determining clonality of a cell culture are provided. Also provided are systems employing the above methods in high throughput sample screening. | 05-23-2013 |
| 20130149739 | DETECTION AND ENUMERATION OF MICROORGANISMS - A method for detecting and enumerating viable microorganisms in a sample suspected of containing said microorganisms: (1) contacting said microorganisms of said sample with repair compounds and a growth medium, and (2) incubating the product of step (1), and (3) detecting and enumerating said microorganisms, in which the microorganisms are of the species | 06-13-2013 |
| 20130157306 | METHOD FOR DETERMINING A DEGREE OF INFECTION - A method for determining a degree of infection comprising the steps of i) preparing an un-isolated sample by adding a differentiating marker, suitably a meta-chromatic stain such as, for example, acridine orange to mammalian milk in an amount sufficient to provide a differentiation between cell types; ii) measuring a differential somatic cell count on the sample by means of a cytometer having a detection system sensitive to differences in the differentiating marker resulting from the marker becoming differently associated with different cell types in the sample; and iii) determining an indication of a degree of infection dependent on the measured differential cell count. | 06-20-2013 |
| 20130164778 | TWO STEP SAMPLE LOADING OF A FLUID ANALYSIS CARTRIDGE - A two-step method for loading a fluid sample into a disposable fluid analysis cartridge is described. First, capillary action may be used to initially draw a sample through a sample introduction port and into a sample collection reservoir provided in the fluid analysis cartridge. Once the fluid sample has been drawn into the sample collection reservoir by capillary action, a negative pressure may be applied to the cartridge to pull the sample from the sample collection reservoir and into a sample loading channel. A valve may be disposed between the sample collection reservoir and the sample loading channel to prevent backflow of sample into the sample collection reservoir and to retain sample in the sample loading channel | 06-27-2013 |
| 20130164779 | DISPOSABLE CARTRIDGE FOR FLUID ANALYSIS - A disposable blood analysis cartridge may include a sample collection reservoir, an absorbance measurement channel, and an optical light scattering measurement channel. One or more valves may be disposed between the sample collection reservoir and the absorbance measurement channel and/or the optical light scattering measurement channel. A negative pressure may be applied to the cartridge to pull sample from the sample collection reservoir through the one or more valves and into the absorbance measurement channel and/or the optical light scattering measurement channel. Once the sample is pulled into the absorbance measurement channel and/or the optical light scattering measurement channel, the one or more valves may be closed. With the one or more valves closed, and in some cases, a pusher fluid may be provided to push the fluid sample to other regions of the disposable fluid blood analysis cartridge. | 06-27-2013 |
| 20130164780 | Drug Identification Method - The invention relates to a method to identify compounds exert a transient effect(s) on one or more biological mediators of acute biological responses to acute stimuli or biological insult in subjects exposed to an acute stimulus or biological insult such as systemic exposure to bacterial LPS. The compounds are identified by measuring the effect of the test compound on the mediator of the acute stimulus or biological insult at times when an acute effect(s) to the stimulus or insult is occurring. | 06-27-2013 |
| 20130196369 | Methods of Culturing Retinal Pigmented Epithelium Cells, Including Xeno-Free Production, RPE Enrichment, and Cryopreservation - The production of high quality retinal pigmented epithelium (RPE) cells is necessary for research and potential therapeutic uses. Especially desirable are methods for the production of RPE cells using xeno-free culture conditions. Disclosed herein are novel methods for the production of RPE cells from pluripotent cells with high yields, including xeno-free production methods. Also provided are methods of efficiently isolating RPE cells from cultures containing heterogeneous cell types, allowing for substantially pure RPE cell cultures to be established. Additionally, novel methods for the cryopreservation of RPE cells are provided. | 08-01-2013 |
| 20130244274 | SAMPLE PROCESSING APPARATUS AND REAGENT INFORMATION INFORMING METHOD - A sample processing apparatus comprises a sample processing unit that carries out processing of a sample using a reagent, an acquiring device that acquires reagent information regarding a reagent in a reagent container provided to the reagent container, an audio output unit that outputs audio, and a control device that manages the reagent information of the reagent in a state of being used by the sample processing unit. The audio output unit outputs audio guidance regarding the acquired reagent information when the acquiring device acquires new reagent information regarding a reagent not in a state of being used by the sample processing unit. | 09-19-2013 |
| 20130252277 | METHOD OF OPERATION OF FERMENTATION OF CARBON MONOXIDE CONTAINING GASEOUS SUBSTRATE - The present disclosure provides methods of gaseous substrate fermentation comprising: adding gaseous substrate into an aqueous medium in a bioreactor. The methods of the present disclosure comprise: measuring cell density; adjusting input of gaseous substrate to increase cell density; changing specific CO uptake in predetermined amounts. | 09-26-2013 |
| 20130260417 | BIOCHEMICAL SENSOR FOR QUANTITATIVE SIMULTANEOUS MULTI-SPECIES BACTERIA DETECTION IN SITU - Methods for detecting the concentration of one or more target organisms in a water sample. The methods involve adding a tagged reagent to a water sample, wherein the tagged reagent is water soluble; and determining a concentration of at least one bacteria in the water sample based on an intensity of an emission emitted from the water sample in response to exposure to light having a known wavelength. An apparatus including a reaction chamber; a reversible pump, a reagent source comprising a fluorophore-tagged reagent, a light source, an optical detector disposed to detect fluorescence emitted from the reaction chamber in response to light emitted from the light source; a processor configured to communicate with the reversible pump, the reagent source, the light source, and the optical detector, the processor being configured to determine the concentration of one or more target organisms in a water sample, and optionally transmit the resulting data to a remote location. | 10-03-2013 |
| 20130273599 | PHOTO-COUPLED DATA ACQUISITION SYSTEM AND METHOD - The photo-coupled data acquisition system can have a container having a contour wall extending upwardly from a closed bottom, for containing a sample therein, a light emitter operable to emit diffused light into the container at an initial intensity, a photodetector operable to detect a reflected intensity of the diffused light, and a structure connected to the contour wall and holding the light emitter and the photodetector at a predetermined height above the bottom of the container and in an orientation facing inside the container, wherein during operation of the system, the initial light intensity is attenuated by the sample and the reflected intensity thereof can be correlated to an information value concerning a variable of interest of the sample. | 10-17-2013 |
| 20130337500 | SYSTEM AND METHOD FOR ISOLATION OF CELLS - In accordance with an embodiment of the invention, there is provided a microfluidic device for isolating cells from a biological fluid. The device comprises an inlet receiving the biological fluid flowed into the device, and at least one array of a plurality of isolation wells receiving the biological fluid from the inlet. At least one isolation well of the plurality of isolation wells comprises a cell trap of a size and shape suitable to mechanically isolate a cell within the cell trap. The cell trap comprises at least one gap of a size and shape suitable to prevent passage of the cells to be isolated but to permit passage of other components of the biological fluid through the cell trap. | 12-19-2013 |
| 20130337501 | CELL ANALYZING APPARATUS AND CELL ANALYZING METHOD - A cell analyzing apparatus includes: a histogram acquirer which is configured to perform measurement of a number of nuclear stained cells, and which, by using a result of the measurement, is configured to acquire a histogram indicating a fluorescence intensity; and an analysis controller which is configured to apply frequency analysis on data of the histogram acquired by the histogram acquirer, and which is configured to determine existence/nonexistence of cancer cells based on a result of the frequency analysis. | 12-19-2013 |
| 20130344534 | Biological Particle Analyzer and Method of Analyzing Biological Particles - A biological particle analyzer is disclosed and includes a microchannel including an end coupled to a first drive electrode, another end coupled to a second drive electrode, a first detection area at an upstream location and an excitation area at a downstream location for containing particles flowing from the upstream location to the downstream location inside the microchannel, a first detection circuit coupled to the first detection area for outputting a first detection result when at least one particle has arrived at the first detection area, a light emission source, and a control module coupled to the first detection circuit and the light emission source for determining when to turn on or off the light emission source according to the first detection result. | 12-26-2013 |
| 20140024073 | BIO-MEMS FOR DOWNHOLE FLUID ANALYSIS - A method and apparatus for observing a biological microelectromechanical systems response to a fluid including combining an activator and a fluid wherein the fluid comprises a component from a subterranean formation, exposing the combined activator and fluid to a sensor in a wellbore to observe a biological microelectromechanical systems response, and integrating data from the observing with petrophysical data. A method and apparatus for observing a biological microelectromechanical systems response to a fluid including a housing comprising a biological microelectromechanical observation material, a signal analyzer in communication with the material, and a fluid preparation device positioned to allow fluid to flow to the surface, wherein the fluid comprises a component from a subterranean formation. | 01-23-2014 |
| 20140024074 | YEAST CONCENTRATION AND VIABILITY MEASUREMENT - The invention generally relates to analyzing yeast viability. More particularly, the invention relates to efficient and effective methods and compositions for accessing and measuring viability and concentration of yeast cells. | 01-23-2014 |
| 20140030756 | IDENTIFYING AND ENUMERATING EARLY GRANULATED CELLS (EGCS) - Methods and systems for automatically identifying and enumerating early granulated cells (EGC) in blood samples are disclosed. In one embodiment a method for identifying EGC in a blood sample includes analyzing white blood cells of the blood sample using a low angle light scatter (LALS) parameter, separating the EGCs from the other white blood cells using the LALS parameter, and enumerating the separated EGCs. | 01-30-2014 |
| 20140038230 | A SIMPLE AND AFFORDABLE METHOD FOR IMMUNOPHENOTYPING USING A MICROFLUIDIC CHIP SAMPLE PREPARATION WITH IMAGE CYTOMETRY - The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and system disclosed herein largely overcome these limitations. The system includes a simple system for CD4 and CD8 counting in point-of-care HIV staging within resource poor countries. Unlike previous approaches, no sample preparation is required with the sample added directly to a chip containing dried reagents by capillary flow. A large area image cytometer consisting of an LED module is used to excite the fluorochromes PerCP and APC labeled targets and a monochrome CCD camera with a combination of two macro lenses captures images of 40 mm | 02-06-2014 |
| 20140038231 | ISOLATION AND CHARACTERIZATION OF TUMOR CELLS USING SHEAR STRESS MEASUREMENTS - Methods for isolating viable cancer cells from a sample that comprises a mixture of cancerous cells and normal (non-cancerous) cells are provided. In the methods, a fluid preparation comprising a mixture of cancerous and normal cells is repeatedly exposed to fluid shear stresses, whereby the repeated exposure to the fluid shear stresses preferentially imparts fluid shear stress-resistance to the cancerous cells. | 02-06-2014 |
| 20140065665 | Method and System for Analyzing a Blood Sample - Methods, systems, and computer program products for the analysis of a blood sample are disclosed. One embodiment is a method of detecting and enumerating hard-to-ghost cells in a blood sample. Another embodiments is a method of analyzing reticulocytes in a blood sample. Methods of using blood count parameters are also provided. | 03-06-2014 |
| 20140093911 | METHOD AND APPARATUS FOR IMAGE-BASED PREDICTION AND SORTING OF HIGH-PERFORMING CLONES - A method of predictive identification and separation of high-performing cells from a mixed population of cells includes distributing cells belonging to the mixed population to a plurality of open chambers; identifying open chambers containing desired cells and open chambers containing undesired cells; selectively sealing at least one open chamber containing undesired cells; and recovering the desired cells from the open chambers. Cells can be predictively assigned as desired or undesired based on an automated image analysis algorithm. | 04-03-2014 |
| 20140127744 | METHODS FOR IMPROVING IN VITRO MEASUREMENTS USING BOYDEN CHAMBERS - Apparatus and methods to improve the Boyden chamber used in cellular biological measurements, allowing quantitative optical microscopy of biological cells in situ without using fluorescent probes or optical staining. In the preferred embodiment, a thin porous membrane separating top and bottom reservoirs includes an array of precisely positioned micropores pores manufactured using a laser-based photo-machining (ablation) process. The membrane may be composed of polyethylene terephthalate (PET), polycarbonate, polyimide, polyether ether ketone (PEEK) or other appropriate material. The pores formed in the membrane may have diameters in the range of 1 to 15 microns and spaced apart at a distance ranging from 10 to 200 microns. A plurality of upper and lower reservoirs may be provided to form a multi-well plate. The invention finds application in a wide range of potential biological applications where Boyden chamber geometries are currently used including co-culture studies, tissue remodeling studies, cell polarity determinations, endocrine signaling, cell transport, cell permeability, cell invasion and chemotaxis assays. | 05-08-2014 |
| 20140162310 | RAPID DETECTION OF VIABLE BACTERIA SYSTEM AND METHOD - An improved system and method is provided for detecting viable bacteria in a suspension sample. A sample of a suspension in which bacterial presence is suspected is collected from a source and a portion of the sample transferred to a microfluidic unit. A series of analysis signals at different frequencies are applied to the sample portion. An impedance is measured via a signal analyzer for the sample portion for each of the analysis signals to define an impedance data set. An initial bulk capacitance value is determined for a model circuit based on the impedance dataset. After a predetermined time period, a new bulk capacitance value is determined for on another portion of the sample. The difference between the new bulk capacitance and the initial bulk capacitance value is compared to a threshold value to determine if viable bacterial is present in the sample. | 06-12-2014 |
| 20140178925 | Nanostructured Biomimetic Device with Contour Map of Multiple Variable Correlation Method to Visually Display the Cancer Progresses - The present invention provides a novel electrochemical device and a cancer cell heat release map method to visually display the cancer progresses. The novel device comprises an electrode having a nanopore biomimetic electron-relay network with imidazolium-ATP of cancer cell-water-pyridine at the active sites that mimic the electron-relaying between His 516 and N(5)-FAD of GOx for the purpose to selectively detect triple-negative breast cancer cell at single cell concentration and it rejects brain cancer cell. The device is fast in millisecond to detect cancers without sample preparation and without interference from other substances, such glucose and proteins under reagent-free conditions. A unique biomarker of the ratio of “Action/Resting” cell membrane potential can be used to monitor the cancer progress against the normal cells. A visual contour map of a multiple variable correlation method provided to assess the heat release from the cancer cells against the normal cells is presented. The device for a potential therapeutic application was demonstrated by discharge voltage pulses from the live cancer cells with release extra energy that the cells possessed until it returns to a normal status in terms of normal cell membrane action/resting potential ratio. | 06-26-2014 |
| 20140178926 | Optical Method and Device for the Detection and Enumeration of Microorganisms - A new device and method for detecting the presence of living microorganisms in test samples are described. The device comprises a container with at least one section transparent to light, a growth zone located in said container containing a mixture of growth media capable of supporting growth of the microorganisms, and at least one indicator substrate that changes its optical properties due to growth of the microorganisms. A detection zone is located in the container adjacent to the transparent section, and a barrier layer comprising porous solid material separates the two zones, allowing diffusion of molecules and ions of metabolic by-products of the organisms, while preventing microorganisms and particulate matter of the test sample from penetrating into the detection zone. | 06-26-2014 |
| 20140199725 | MICROORGANISM DETECTING SYSTEM AND MICROORGANISM DETECTING METHOD - A microorganism detecting device is provided for illuminating a solution with light, for detecting fluorescent light that is produced by a microorganism included in the solution, and for detecting the number of microorganisms included in the solution. The microorganism detecting device includes a relationship storing device that stores a correlation relationship between a number of microorganisms in a solution and a number of colonies formed by the microorganisms on a culture medium, and a converting portion that uses the correlation relationship to convert, into a number of colonies that may be formed, the number of microorganisms detected by the microorganism detecting device. | 07-17-2014 |
| 20140220622 | DIGITAL HOLOGRAPHIC MICROSCOPY APPARATUS AND METHOD FOR CLINICAL DIAGNOSTIC HEMATOLOGY - An apparatus, method, and apparatus for hematology analysis comprising using a holographic microscope, in one embodiment a transmission-type holographic microscope. In one aspect, laser light is provided and split into first and second sample beams, the first sample beam for imaging with a first magnification, the second sample beam for imaging with a second magnification. The first and second sample beams are passed through a sample volume requiring hematology analysis. The first and second sample beams are combined with a reference beam and captured for digital analysis. The present invention enables adequate blood cell type differentials with a single, easily implemented, cost-effective holographic technique. | 08-07-2014 |
| 20140220623 | METHODS AND COMPOSITIONS FOR ESTIMATING SOIL MICROBIAL LOAD - Methods and compositions for the preparation of soil samples and the determination of the number of microbes within a soil sample are disclosed. The methods include measuring, solubilizing, bleaching and filtering soil samples and measuring the turbidity of the filtered solution. The turbidity of the sample can be determined by visual inspection of a Secchi disk viewed through the column liquid sample in a transparent tubular cylinder, or by using a cell phone camera and application. Devices and kits for the rapid, cost efficient determination of microbial numbers in the field setting are disclosed. The disclosed devices and methods can also be used for applications other than determination of microbial numbers. | 08-07-2014 |
| 20140242633 | URINE SAMPLE ANALYZER AND SAMPLE ANALYZING METHOD - A urine analyzer capable of operating in a urine measurement mode and a body fluid measurement mode, the urine analyzer includes a specimen preparing section configured to prepare a measurement specimen and a detecting section configured to derive signals of particles in the measurement specimen supplied from the specimen preparing section. A computer and a memory including programs on a computer-readable medium that enable the computer to execute operations to control the specimen preparing section and the detecting section in the urine measurement mode and in the body fluid measurement mode. | 08-28-2014 |
| 20140273075 | METHODS, SYSTEMS AND DEVICES FOR DETERMINING WHITE BLOOD CELL COUNTS FOR RADIATION EXPOSURE - Systems and methods are provided for imaging at least one eye with optical components and processing the image of the at least one eye to determine radiation exposure. An image of one or more eyes may be received. The systems and methods may distinguish blood cell types in the image; quantify circulating levels of a selected blood cell type; output a number of blood cells per volume of the selected blood cell type; and determine a radiation dose based on the number of blood cells per volume of the selected blood cell type. | 09-18-2014 |
| 20140273076 | DYNAMIC RANGE EXTENSION SYSTEMS AND METHODS FOR PARTICLE ANALYSIS IN BLOOD SAMPLES - For analyzing a sample containing particles of at least two categories, such as a sample containing blood cells, a particle counter subject to a detection limit is coupled with an analyzer capable of discerning particle number ratios, such as a visual analyzer, and a processor. A first category of particles can be present beyond detection range limits while a second category of particles is present within respective detection range limits. The concentration of the second category of particles is determined by the particle counter. A ratio of counts of the first category to the second category is determined on the analyzer. The concentration of particles in the first category is calculated on the processor based on the ratio and the count or concentration of particles in the second category. | 09-18-2014 |
| 20140273077 | DEVICE FOR THE ASEPTIC EXPANSION OF CELLS - A closed system suitable for the aseptic culturing therapeutic cells comprises a vessel comprising: a gas permeable portion suitable for supporting cell growth and allowing delivery of gases to the cells during culturing, a vent comprising a conduit having an exterior orifice and an interior orifice spaced apart therefrom, the vent extending from the exterior of the system into the internal volume of the vessel and terminating therein with the interior orifice, wherein the interior orifice is arranged such that during filling and emptying of liquid medium it is not susceptible to blockage by liquid, the exterior orifice is adapted to connect to an aseptic filter thereby allowing passage of gases through the filter into the vessel or out of the vessel, as required to achieve the entry and exit of fluids and cells into the vessel, a port or ports adapted to allow introduction of fluids and cells aseptically into the vessel, and a port or ports adapted to allow fluids to exit the system without exposing the system to the external environment and adapted such that cells grown therein may exit the system under gravity when the system is orientated to put the cells in fluid communication with the exit port and the latter is opened. The present system facilitates the aseptic manufacturing of cells for use in therapy, without the need for a clean-room environment because no open processing steps are required. | 09-18-2014 |
| 20140295490 | FETAL RED BLOOD CELL DETECTION - A device for analyzing a maternal blood sample for quantification of the percentage of fetal red blood cells present with respect to the number of maternal red blood cells includes reagents for mixing with the biological sample, a microfluidic chip, 5 fluid reservoirs, a pumping system, an image acquisition system, an image analysis system, and an electronic control board. The microfluidic channel can confine the objects of interest to a monolayer, and may trap them in an organized array for analysis. The device uses a reduced sample volume and microfluidic pumping and imaging techniques throughout. The disclosed invention holds distinct advantages over the current state of the art in fetal red blood cell quantification in a maternal blood sample by producing faster results, removing operator error, reducing 10 costs, and providing overall simplification of the testing and analysis procedure. | 10-02-2014 |
| 20140302551 | URINE SAMPLE TESTING APPARATUS AND APPARATUS FOR PROCESSING MEASUREMENT RESULT OF URINE SAMPLE - A urine sample testing apparatus comprises: a urine qualitative measuring section configured to acquire a measurement result for each of a plurality of urine qualitative measurement items; a urine sediment measuring section configured to acquire a measurement result for each of a plurality of urine sediment measurement items; an operation part that is operable by a user to specify a combination of one of the plurality of urine qualitative measurement items and one of the plurality of urine sediment measurement items; an information processing unit configured to determine whether or not a first measurement result of the urine sample obtained by the urine qualitative measuring section and a second measurement result of the urine sample obtained by the urine sediment measuring section have a predetermined relationship with respect to the urine qualitative measurement item and the urine sediment measurement item included in the specified combination. | 10-09-2014 |
| 20140315242 | Microfluidic Chamber Assembly for Mastitis Assay - The present invention relates to a device and method for the detection of mastitis or other disease from a body fluid of a mammal for example from cow's milk. The device and method relates to a wedge microfluidic chamber for using a minimal amount of fluid and being able to use the device to observe leukocytes in a mono-layer for the purpose of disease detection, cell counts or the like. | 10-23-2014 |
| 20140322750 | BLOOD ANALYZER, BLOOD ANALYSIS METHOD AND HEMOLYTIC AGENT - This blood analyzer includes a sample preparation portion preparing a measurement sample free from a labeling substance from a blood sample and a hemolytic agent free from a labeling substance, a light information generation portion generating fluorescent information and at least two types of scattered light information from the measurement sample and a control portion performing a first classification of white blood cells in the measurement sample into at least four groups of monocytes, neutrophils, eosinophils and others on the basis of the fluorescent information and the two types of scattered light information. | 10-30-2014 |
| 20140356903 | HEMATOLOGICAL ANALYZER, METHOD FOR ANALYZING BODY FLUID AND COMPUTER PROGRAM PRODUCT - A hematological analyzer for measuring blood, sets a body fluid measurement mode; receives a measurement start instruction; irradiates a measurement sample with light and obtains optical information from cells contained in the measurement sample; and classifies at least white blood cells and nucleated cells other than white blood cells contained in the measurement sample, and counts the white blood cells and nucleated cells other than white blood cells based on the optical information obtained from the cells in the measurement sample prepared from a body fluid sample and white blood cell measuring reagent when the body fluid measurement mode has been set and the measurement start instruction has been selected, is disclosed. A method for analyzing body fluid and a computer program product are also disclosed. | 12-04-2014 |
| 20150037835 | SYSTEM AND METHOD FOR AUTOMATED DIAGNOSIS - An automated microscope apparatus comprises an outer housing having an external wall; optionally but preferably an internal wall in the housing configured to form a first compartment and a separate second compartment in the outer housing; a microscope assembly in the housing (preferably in the first compartment); a microprocessor in the housing (preferably in the second compartment), and (optionally but preferably) a heat sink mounted on the housing external wall, preferably adjacent the second compartment, with the microprocessor thermally coupled to said heat sink and operatively associated with the microscope assembly. Systems and methods employing the same are also described, along with component parts thereof. | 02-05-2015 |
| 20150056649 | METHOD FOR QUANTIFYING CELL OF INTEREST IN BLOOD, AND METHOD FOR EVALUATING SYSTEM FOR QUANTIFYING SAID CELL - A method for quantifying cells of interest potentially contained in a blood-derived sample in cases of their quantification after separation from the blood-derived sample may enable accurate quantification of the cells without causing underestimation of the cell number. The quantification method is a method for quantifying specific cells of interest, the method may include: (A) separating a blood-derived sample containing a known number (P0) of specific resin particles P into at least two layers including a layer of erythrocytes and a layer of cells other than erythrocytes; (B) extracting the layer of cells other than erythrocytes and counting the number of cells of interest and the number (P1) of the resin particles therein; and (C) correcting the number of cells of interest by multiplying the number of cells of interest by P0/P1. | 02-26-2015 |
| 20150072377 | CELL CULTURING DEVICE - The invention features devices and kits for capturing and culturing microorganisms (e.g., bacteria, fungi, or protists) and methods of using the devices and kits to detect microorganisms in environmental and other samples. The device includes a nutrient media having a flat growth area on which microorganisms can grow. Samples are collected by contacting the device with any environmental sample, e.g., rolling device on a work surface or exposing device to air, or by filtering a sample through a membrane. Microorganisms deposited on the membrane derive nutrients from the underlying media and grow into colonies that can then be detected using methods known in the art. The detected colonies can be imaged digitally or with film. | 03-12-2015 |
| 20150072378 | Systems and Methods for Incubating Samples - Provided are incubation systems for incubating samples for an assay. In certain aspects, the systems include a rotary platform, a sample container positioned at a peripheral edge of the platform, a plurality of activity sites including at least a first and a second activity site, a processor, and a memory that includes instructions. When executed by the processor, the instructions cause the system to rotate the rotary platform to move the sample container from the first activity site to the second activity site, extract a first portion of the sample from the sample container at the first activity site for a measurement based on a first incubation time period, and extract a second portion of the sample from the sample container at the second activity site for a measurement based on a second incubation time period, the second incubation time period being longer than the first incubation time period. Methods for incubating samples, e.g., methods which employ the systems of the present disclosure, are also provided. | 03-12-2015 |
| 20150099274 | METHOD AND SYSTEM FOR USE IN MONITORING BIOLOGICAL MATERIAL - An optical system for determining the concentration of a metabolic gas in a container sealed to biological contamination and enclosing a biological material. The optical system has a broadly tunable coherent infrared light source, a detection module, and a control system connected to the light source and detection module and operates the light source, to receive and analyze the data provided by said detection module, and to process the data indicative of the concentration of said metabolic gas in said sealed container. | 04-09-2015 |
| 20150111244 | DETECTION OF MICROORGANISMS INVOLVED IN METABOLISM OF ORGANIC ENVIRONMENTAL POLLUTANTS - A method and point of use kit to determine under field conditions the presence and/or amount of a selected | 04-23-2015 |
| 20150118708 | METHOD AND DEVICE FOR DETECTING BACTERIA AND DETERMINING THE CONCENTRATION THEREOF IN A LIQUID SAMPLE - A method for detecting bacteria and determining the concentration thereof in a liquid sample includes the steps of taking an optical section through a container holding a volume of the liquid sample at a predetermined field of view and at a predetermined focal plane depth or angle and after a period of time has elapsed to allow non-bacteria in the sample to settle to the bottom of the container. Since bacteria auto arranges in the liquid sample, forming a lattice-like grid pattern, an optical section through the volume of auto-arranged bacteria may be used to measure the quantity of bacteria residing in that section. A container for holding the liquid sample has particular structure which aids in separating the non-bacteria from the bacteria. | 04-30-2015 |
| 20150125899 | FLUORESCENCE-ASSISTED COUNTING APPARATUS FOR QUALITATIVE AND/OR QUANTITATIVE MEASUREMENT OF FLUORESCENTLY TAGGED PARTICLES - A fluorescence-assisted counting apparatus for quantitative and/or qualitative assessments of a population of fluorescently stained particles in a specimen device retaining fluorescently stained particles, wherein the apparatus includes a detector module and a light source. The detector module includes a lens, a first optical filter and a detector, wherein the detector detects a fluorescence response. The lens includes a field of view taking in at least a portion of the fluorescently stained particles within the specimen device. The light source, further including a second optical filter, shines light in a light path that encompasses at least a portion of the particles. | 05-07-2015 |
| 20150125900 | SAMPLE ANALYZER AND COMPUTER PROGRAM PRODUCT - A sample analyzer prepares a measurement sample from a blood sample or a body fluid sample which differs from the blood sample; measures the prepared measurement sample; obtains characteristic information representing characteristics of the components in the measurement sample; sets either a blood measurement mode for measuring the blood sample, or a body fluid measurement mode for measuring the body fluid sample as an operating mode; and measures the measurement sample prepared from the blood sample by executing operations in the blood measurement mode when the blood measurement mode has been set, and measuring the measurement sample prepared from the body fluid sample by executing operations in the body fluid measurement mode that differs from the operations in the blood measurement mode when the body fluid measurement mode has been set, is disclosed. A computer program product is also disclosed. | 05-07-2015 |
| 20150147777 | PASSIVE MICROFLUIDIC METERING DEVICE - An integrated fluidic device comprising includes an input chamber that provides an input of a sample fluid, and a first overspill chamber in fluid communication with the input chamber. A metering conduit is in fluid communication with the fluid input chamber and the first overspill chamber. The metering conduit meters a first metered volume of fluid from the sample fluid, and the first overspill chamber receives fluid in excess of the first metered volume of fluid. A second overspill chamber is in fluid communication with the metering conduit. The metering conduit meters a second metered volume of fluid from the first metered volume of fluid, and the second overspill chamber receives fluid from the first metered volume of fluid in excess of the second metered volume of fluid. The second overspill chamber has a fluid actuated closable valve for controlling the metering of the second metered volume of fluid. | 05-28-2015 |
| 20150291994 | MICROORGANISM ENUMERATION METHODS - This disclosure provides methods for enumeration of microorganisms within a freeze-dried powder suspected of containing Lactobacilli or Bifidobacteria. | 10-15-2015 |
| 20150293013 | SYSTEMS AND METHODS FOR MONITORING CHEMICAL PROCESSES - A method of monitoring a fluid includes containing the fluid within a flow path, the fluid having a chemical reaction occurring therein. At least one integrated computational element is optically interacted with the fluid, thereby generating optically interacted light. An output signal is then produced based on the optically interacted light that corresponds to a characteristic of the chemical reaction. | 10-15-2015 |
| 20150309009 | SYSTEMS AND METHODS FOR IN VITRO AND IN VIVO IMAGING OF CELLS ON A SUBSTRATE - Disclosed herein are generally to methods and systems that facilitate imaging of cells on a substrate and more particularly to pre-implantation (in vitro) and post-implantation (in vivo) imaging of cell-seeded substrates implanted in target tissues in the context of stem cell therapy. | 10-29-2015 |
| 20150317506 | METHOD FOR IMAGING BIOLOGIC FLUID SAMPLES USING A PREDETERMINED DISTRIBUTION - A method for analyzing a biologic fluid sample includes the steps of: a) providing a spatially mapped chamber; b) providing a predetermined repeatable non-uniform spatial distribution of one or more constituents within the sample, which distribution indicates the presence or absence of a statistically significant number of constituents within the sample in each chamber sub-region; c) selecting one or more image techniques for each sub-region based on the presence or absence of the statistically significant number of one or more constituents in that sub-region as indicated by the distribution; d) creating image data representative of the biologic fluid sample in each sub-region, using the one or more image techniques selected for that sub-region; and e) analyzing the sample. | 11-05-2015 |
| 20150322479 | APPARATUS AND METHOD FOR QUANTIFICATION OF REPLICATIVE LIFESPAN AND OBSERVATION OF SENESCENE - The present invention teaches an apparatus and method for the automated study of senescence of cells. The apparatus includes a plurality of plates, customized with a flow-through hole, arranged on a platform. A microscope with integrated camera is positioned above the platform and both microscope and platform are coupled to a rotation mechanism to allow relative rotation between the platform and the microscope. Cells are anchored magnetically, chemically, or electrostatically to the plates and are treated to a controlled environment. Daughter cells born from the mother cell are observed by the microscope and then washed, using a wash fluid, through the flow-through hole in the plate. A processor automates the process and allows for a user to input customizable test parameters and value thresholds to indicate a test is completed. The processor also organises test data in the form of a spreadsheet for simple modelling and data manipulation. | 11-12-2015 |
| 20150323519 | DISPOSABLE CHAMBER FOR ANALYZING BIOLOGIC FLUIDS - An apparatus for analyzing biologic fluid is provided that includes a first planar member, a second planar member, and at least three separators. At least one of planar members is transparent. The separators are disposed between the members, and separate the members to form a chamber having a height. At least one of the members or separators is sufficiently flexible to permit the chamber height to approximate the mean size of the separators. During use, the biologic fluid to be analyzed is disposed within the chamber. | 11-12-2015 |
| 20150330963 | METHOD AND APPARATUS FOR DETERMINING WHITE BLOOD CELL COUNTS - Embodiments of the present invention encompass automated systems and methods for analyzing white blood cell parameters in an individual based on a biological sample obtained from blood of the individual. Exemplary techniques involve correlating aspects of direct current (DC) impedance, radiofrequency (RF) conductivity, and/or light measurement data obtained from the biological sample with an evaluation of white blood cell conditions in the individual. | 11-19-2015 |
| 20150337350 | AUTOMATED VIABILITY TESTING SYSTEM - The invention provides an automated device for accessing the viability of a wide range of organisms based on the metabolic production of fluorescent products from non-fluorescent substrates. Also provide are methods for detecting contaminants in a fluid and measuring the viability of organisms in a fluid or liquid. Components of the invention include the incorporation of a reusable filter to concentrate the organisms, the back flush of the filter to collect the organisms for assay, and the addition of the substrate in a fluorescent detection chamber to detect the enzymatic activity produced by viable organisms to detect the presence of such organisms. | 11-26-2015 |
| 20150353916 | GALVANOTAXIS ASSAY FOR QUANTITATIVE ASSESSMENT OF THE METASTATIC POTENTIAL OF CANCER CELLS - An apparatus and method for accelerating and/or inhibiting the migration of cells by applying a time-varying magnetic field to induce eddy currents that promote electrotaxis (galvanotaxis) of cells without the need for chemokines or glucose. The present invention can also be used to study and quantify the metastatic potential of different cell lines. | 12-10-2015 |
| 20150353983 | MEANS, METHOD AND COMPUTER PROGRAM PRODUCT FOR DETERMINING THE CONCENTRATION LEVEL OF MICROORGANISMS DURING A FLUID ANALYSIS - A locating means ( | 12-10-2015 |
| 20150368690 | VIABILITY STAINING METHOD - The invention relates to a method of detecting viable cells in a cell sample, using a membrane permeable fluorescent label that permeates both viable and non-viable cells and a membrane impermeant quencher that selectively permeates non-viable cells. | 12-24-2015 |
| 20150369793 | BLOOD ANALYZER, BLOOD ANALYZING METHOD, AND NON-TRANSITORY STORAGE MEDIUM - A blood analyzer comprises a sample preparing part, a light source, a light receiving part, and a processing part configured to discriminate and count reactive B lymphocytes based on at least one of fluorescent light signals and forward scattered light signals. | 12-24-2015 |
| 20160002697 | MICROORGANISM DETECTION METHOD - Provided is a microorganism detection method which is highly versatile and applicable to both the test for the detection of specified microorganisms and the microbial enumeration test under the same test conditions for Kampo extract preparations and crude drugs containing a variety of antimicrobial substances, is able to effectively reduce the influence of antimicrobial substances in a test sample, and may detect target microorganisms with high precision. This method is a microorganism detection method in a test sample having antimicrobial activity, including a step of preparing a sample liquid containing a test sample having antimicrobial activity and a step of culturing a target microorganism in a culture medium, the method including allowing a (meth) acrylic acid ester-based synthetic adsorbent to act on the sample liquid and/or the culture medium. | 01-07-2016 |
| 20160002698 | Isotonic Buffered Composition and Method That Enables Counting of Cells - The present invention discloses multi-purpose metering fluid/rinse reagents for use in automated cellular analyzers that use liquid volumetric metering. The compositions contain a chelating agent, an ionizing salt, optionally a stabilizing ion, a buffer, a non-hemolytic surfactant, and optionally an antimicrobial agent. Advantageously, the compositions produce less than one part-per-million of formaldehyde over the course of one year. Methods for using the compositions are also described. | 01-07-2016 |
| 20160011174 | Assay for Determining the Cell Number in Cultured Cells | 01-14-2016 |
| 20160018346 | BLOOD CONDITION ANALYZING DEVICE, BLOOD CONDITION ANALYZING SYSTEM, BLOOD CONDITION ANALYZING METHOD, AND BLOOD CONDITION ANALYZING PROGRAM FOR CAUSING COMPUTER TO EXECUTE THE METHOD - There is provided a blood condition analyzing device including an erythrocyte quantitative evaluation unit configured to evaluate a hematocrit value and/or a hemoglobin amount on the basis of an electrical characteristic of a blood sample at a frequency of 2 to 25 MHz. | 01-21-2016 |
| 20160041083 | HEMATOLOGY SYSTEMS AND METHODS - Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer. Embodiments also encompass systems, compositions, and methods for analyzing a sample containing particles. Particles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed, for example using certain focusing techniques, into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. Blood cell images can be collected and analyzed using dynamic range extension processes and systems. | 02-11-2016 |
| 20160041102 | POLY(METHYL METHACRYLATE)-SUPPORTED POLYDIACETYLENE FILMS AS COLORIMETRIC AND/OR FLUORESCENT DETECTORS - Colorimetric and/or fluorescent detectors, which include a polydiacetylene (PDA) film deposited on a polymethylmetacrylate (PMMA) substrate. There are also colorimetric and/or fluorescent detectors which have a solid organic matrix comprising one or more PDAs deposited on the PMMA substrate, wherein the PDA includes polymerized units of one or more diacetylene monomers, and wherein the matrix further comprises at least one recognition element of an analyte. There are also methods for detecting analytes using this detector, uses thereof and a methods for its preparation. | 02-11-2016 |
| 20160066565 | ISOLATOR DEVICES FOR COLLECTION, PARAMETRICAL CHARACTERIZATION AND LONG-TERM PRESERVATION OF ORGANIC FLUIDS AND/OR MATERIALS CONTAINING STEM CELLS - An improved isolator for collection, parametrical characterization and long-term preservation of samples containing stem cells comprises: a work chamber ( | 03-10-2016 |
| 20160075988 | CULTURE DEVICE AND METHODS FOR ENUMERATING MOLD COLONIES - A thin film culture device for enumerating mold colonies is provided. The device comprises water-resistant first and second substrates with a growth region disposed therebetween, a dry, cold water-soluble gelling agent disposed in the growth region, and an effective amount of a calcium-chelating compound disposed in the growth region. The effective amount of calcium-chelating compound is capable of reducing a rate of lateral enlargement of the colony-forming unit growing in the culture device relative to the rate of lateral enlargement of a colony of the same mold species growing in an otherwise identical culture device that does not contain the effective amount disposed in the growth region, wherein reducing the rate of lateral enlargement of the colony-forming unit does not substantially delay detection of the colony. A corresponding method is also provided. | 03-17-2016 |
| 20160108452 | SEMI-CONTINUOUS CULTURE METHODS - Provided herein are methods of culturing a microorganism. The methods include providing a container comprising one or more microorganisms in a medium, which has a first carbon to nitrogen ratio; culturing the microorganisms until the culture reaches a threshold indicator; harvesting a portion of the culture while maintaining the majority of the culture in the container; and adding fresh medium comprising a second carbon to nitrogen ratio to the container with the majority of the culture comprising the microorganisms. | 04-21-2016 |
| 20160108453 | REPEATED FED-BATCH CULTURE METHODS - Provided herein are methods of culturing a microorganism. The method includes providing a container comprising one or more microorganisms and medium, wherein the microorganisms and medium form a start volume. The microorganisms and medium are cultured until the culture reaches a threshold indicator, wherein culturing comprises feeding one or more carbon sources to the culture and wherein the culture is at a threshold volume when the threshold indicator is reached. The method also includes harvesting a portion of the threshold volume to leave a residual volume that is 40% or less of the start volume and adding fresh medium to the container in an amount to return the volume of the culture to the start volume. | 04-21-2016 |
| 20160122796 | METHOD FOR EXAMINING MICROORGANISMS - A method for examining microorganisms has a sampling preparation step including a fluorescence staining step of stirring and mixing certain amounts of a sample and a fluorescence staining reagent, a still standing step of leaving the solution after the fluorescence staining step to still stand for a certain time, and a dilution step of diluting the solution after the still standing step with a liquid that emits no fluorescence. | 05-05-2016 |
| 20160131658 | METHOD AND KIT FOR ASSESSING VIABLE CELLS - The present invention provides simple, rapid methods and procedures for analyzing cells, hereunder quantitative and qualitative assessment of cells, such as viability. The present invention relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with thiol-containing species present in higher concentrations in intact (e.g., living) cells than in non-intact (e.g., dead, stressed and apoptotic) cells. The present invention also relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with species present in intact and/or non-intact cells. Moreover, the present invention relates to the use of measuring techniques and/or instruments coupled with the use of various optionally substituted reporter compounds. The invention further relates to compositions used in methods for analyzing cells, such as a composition comprising N-(7-dimethylamino-4-methyl-3-coumarinyl)-maleimide (DACM). | 05-12-2016 |
| 20160138071 | METHODS FOR DETECTING THE PRESENCE OF MICROBES - A method and instrument for detecting the presence or absence of a target organism in a test sample is provided. The instrument includes a carousel, a light source, alight detector, and a control system. The carousel is configured to hold a plurality of test sample containers, with each container held by the carousel at a container position. Each of the containers holds an admixture of a test mixture inoculated with the test sample. The light detector is operable to detect light emitted by the light source that has passed through the test sample disposed within the test container. The control system includes a processor. The control system is adapted to produce information indicative of the presence or absence of the target organism in the test sample using signals produced by the light detector. | 05-19-2016 |
| 20160145670 | Microbial Ecology Shift Assay - The disclosure provides assay methods for characterizing the effects of an agent on a microbiome of a subject. Moreover, the disclosure provides methods for practical applications of assay results. The biological sample is extracted and the microbial population is enumerated by using signals or markers specific to the microbial species. The enumerated population is subjected to the action of one or many therapeutic agents and the efficiency is assessed by deriving a score based on the effects in the individual samples and in the population of samples. | 05-26-2016 |
| 20160157760 | QUANTIFYING NEUTROPHIL CONCENTRATION IN BLOOD - The present invention comprises medical diagnostic methods and devices that quantify neutrophil populations in blood by using optical spectroscopy, either ex vivo with collected blood or non-invasively in vivo. In certain embodiments, fluorescent and Raman spectroscopy may be used to distinguish and/or quantify the neutrophils from the other blood components. The methods and devices of the invention advance the detection of sepsis by developing a point of care diagnostic device capable of rapid and/or real-time quantification of neutrophils. Other embodiments of the technology are also envisaged, particularly for analysing blood constituents both endogenous and administered. | 06-09-2016 |
| 20160160260 | Multi-Sample Laser-Scatter Measurement Instrument With Incubation Feature And Systems For Using The Same - An optical measurement instrument is an integrated instrument that includes an optical cavity with a light source, a sample cuvette, and an optical sensor. The light source and sensor are on a bench that is on a translational or rotational mechanical platform such that optical beam can be moved to multiple sample containers. The instrument can be used for taking measurements of organism concentration in multiple samples as a production tool for microbiology. Preferably, the instrument holds multiple, individually-loaded, independent fluid samples and determines bacteria concentration via a forward-scattering signal. The instrument can incorporate onboard incubation to promote bacterial growth in the samples during the test. In another aspect, the instrument can be a part of a network for medical diagnostic testing data where data is stored in a manner that is inherently untainted by patient identifiable information. | 06-09-2016 |
