Isolated Nucleic Acids for Producing High Viscosity Diutan Gums and Methods

Abstract

The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.

Description

CROSS REFERENCE TO RELATED APPLICATIONS

[0001] This application is a divisional application of U.S. application Ser. No. 12/892,561, filed Sep. 28, 2010 which is a continuation of U.S. application Ser. No. 11/264,268, filed Nov. 1, 2005, now U.S. Pat. No. 7,868,167 issued on Jan. 11, 2011. The contents of these applications are hereby incorporated by reference in their entirety.

FIELD OF THE INVENTION

[0002] The present invention describes the production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host Sphingomonas strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.

BACKGROUND OF THE INVENTION

[0003] Polysaccharides or gums are primarily used to thicken or gel aqueous solutions and are frequently classified into two groups: thickeners and gelling agents. Typical thickeners include starches, xanthan gum, diutan gum, welan gum, guar gum, carboxymethylcellulose, alginate, methylcellulose, gum karaya and gum tragacanth. Common gelling agents include gelatin, gellan gum, starch, alginate, pectin, carrageenan, agar and methylcellulose.

[0004] Some polysaccharides, or more particularly stated, biogums, such as xanthan, gellan, welan and diutan have been produced via fermentation from microbes for many years. Such biogums exhibit varied characteristics such as viscosity modification capabilities that have permitted their utilization in many different applications. Included within such a list are gelling agents for foods, such as confectionery jellies, jams and jellies, dessert gels, icings and dairy products, as well as components of microbiological media. Furthermore, thickening agents are utilized for myriad end-use applications to modify the viscosity of target liquids. Of particular interest is the ability of such gums to impart viscosity modification to underground and/or underwater petroleum liquids to facilitate collection thereof, although many other different possible end-uses exist (including cement production, as one example). Different biogums have been produced from different bacterial sources, such as xanthan gum, from Xanthomonas campestris, gellan gum, from Sphingomonas elodea, welan gum from Sphingomonas sp. ATCC 31555, and diutan gum (S-657), from Sphingomonas sp. ATCC 53159. Genetic modifications of such strains have been undertaken in the past to effectuate significant changes in the resultant gum materials produced through the aforementioned fermentation procedures. Such modifications have permitted such changes as removal of acyl groups to create different gum materials exhibiting different physical properties. Generally, such genetic modifications have been of the type to either alter the composition of the target biogum ultimately through altered gene expression within the host organism, or increase the yield of the target biogum, through introduction of a plasmid that exhibits gene amplification alone (such as in U.S. Pat. Nos. 5,854,034, 5,985,623, and 6,284,516, to Pollock et al. and U.S. Pat. No. 6,709,845 to Pollock alone).

[0005] Diutan gum (also known as heterpolysaccharide S-657) is prepared by fermentation of strain Sphingomonas sp. ATCC 53159 and exhibits thickening, suspending, and stabilizing properties in aqueous solutions. Diutan generally exhibits a hexameric repeat unit consisting of four sugars in the backbone (glucose-glucuronic acid-glucose-rhamnose) and a side chain of two rhamnose residues attached to one of the glucose residues. Details of the diutan gum structure may be found in an article by Chowdhury, T. A., B. Lindberg, U. Lindquist and J. Baird, Carbohydrate Research 164 (1987) 117-122. Diutan was shown to have two acetyl substituents per repeat unit within Diltz et al., Carbohydrate Research 331 (2001) 265-270. Both of these references are hereby incorporated by reference in their entirety. Details of preparing diutan gum may be found in U.S. Pat. No. 5,175,278, which is hereby incorporated by reference in its entirety. Diutan may be produced from the Sphingomonas strain by utilizing standard fermentation techniques such as using carbohydrate sources (glucose, maltose, and the like, as non-limiting examples), a nitrogen source, and additional salts.

[0006] The physical characteristics imparted by such a diutan biogum in its wild-type form are desired by certain industries, particularly in terms of its viscosity modification properties and/or water retention characteristics. Unfortunately, diutan has proven difficult to produce cost effectively. Furthermore, such cost issues militate against widespread utilization of diutan currently since the degree of viscosity exhibited by such a biogum is insufficient to supplant other less expensive, but effective, biogums (such as xanthan gum, as one example). As such, it has been an established need to provide a method to produce such an effective diutan at lower cost, at the very least, and/or to provide a manner of producing a biogum of the diutan type that exhibits a significant improvement in physical properties as well. To date, the only mention of production of any types of related sphingans (without any demonstrations for diutan specifically) is in terms of higher yield (within the Pollock et al. patents mentioned above). There has been no discussion or fair suggestion of any manner of providing a method for producing an improved diutan gum of higher molecular weight that exhibits any improvement in viscosity measurements via such a production method.

BRIEF DESCRIPTION OF THE INVENTION

[0007] It has now been realized that amplification of certain novel isolated DNA sequences for diutan biosynthesis within a host Sphingomonas organism not only permits increased production of diutan gum therefrom, but also produces a diutan gum that exhibits increased viscosity properties. Such a novel DNA sequence (that is introduced within a host organism via any well known method, such as, without limitation, a plasmid) thus provides the desired results that have been sought after for diutan synthesis methods. A distinct advantage of such utilization of these genes amplified on a plasmid is the relatively simple nature of incorporating such an isolated DNA sequence into diutan synthesis procedures. Another advantage is the ability to produce such higher viscosity properties for the target diutan gum, while potentially increasing the fermentation production efficiency, if necessary.

[0008] Accordingly, this invention includes a diutan gum exhibiting an improvement in a number of different viscosity measurements. Among these are: i) an intrinsic viscosity of greater than 150, preferably higher than 155, more preferably higher than 160 dL/g; ii) a sea water 3 rpm viscosity greater than 35, preferably higher than 37, more preferably higher than 40, and most preferably higher than 42 dial reading; iii) a sea water 0.3 rpm viscosity greater than 35,000, preferably higher than 39,000, more preferably higher than 40,000, and most preferably higher than 41,000 centipoise (cP); and a PEG low shear rate viscosity greater than 3500, preferably higher than 3700, more preferably higher than 3900, and most preferably higher than 4000 cP. Also, this invention encompasses a method of producing such a diutan gum, as defined in any of those terms above, through, the introduction of a specific cluster of genes into a host Sphingomonas organism and permitting fermentation of said organism to produce a resultant diutan gum.

[0009] Furthermore, this invention encompasses the specific DNA sequences and any vector (such as a plasmid) to provide multiple copies of the genes or increased expression of the genes by use of a stronger promoter, and the like. Additionally, the genetically modified strain of Sphingomonas containing multiple copies of the diutan biosynthetic genes defined by such unique isolated DNA sequences is also encompassed.

[0010] Such a unique isolated DNA sequence has been found to require at least one diutan biosynthetic enzyme being a DpsG polymerase. In another possible embodiment, such a diutan biosynthetic enzyme will include a DpsG polymerase and a glucose-1-phosphate thymidylyltransferase; a dTDP-6-deoxy-D-glucose-3-5-epimerase; a dTDP-D-glucose-4,6-dehydratase; and a dTDP-6-deoxy-L-mannose-dehydrogenase. In yet another possible embodiment such a diutan biosynthetic enzyme will include a DpsG polymerase and a rhamnosyl transferase IV; a beta-1,4-glucuronosyl transferase II; a glucosyl isoprenylphoaphate transferase I; and a glucosyl transferase III. In still another possible embodiment, such a diutan biosynthetic enzyme comprises a dpsG polymerase and polysaccharide export proteins dpsD, dpsC, and dpsE. In yet another possible embodiment, such a diutan biosynthetic enzyme will include a rhamnosyl transferase IV; a beta-1,4-glucuronosyl transferase II; a glucosyl isoprenylphoaphate transferase I; glucosyl transferase III; a glucose-1-phosphate thymidylyltransferase; a dTDP-6-deoxy-D-glucose-3-5-epimrerase; a dTDP-D-glucose-4,6-dehydratase; and a dTDP-6-deoxy-L-mannose-dehydrogenase. Generally, the diutan biosynthetic enzyme of the inventive method and within the inventive product may be selected from the group consisting of polymerase; lyase; rhamnosyl transferase IV; beta-1,4-glucuronosyl transferase II; glucosyl transferase III; polysaccharide export protein; secretion protein; glucosyl-isoprenylphosphate transferase I; glucose-1-phosphate thymidylyltransferase; dTDP-6-deoxy-D-glucose-3-5-epimerase; dTDP-D-glucose-4,6-dehydratase; dTDP-6-deoxy-L-mannose-dehydrogenase and combinations thereof. Further encompassed within this invention then is an isolated nucleic acid molecule (in addition to DNA which may be present on the target chromosome) which encodes at least one diutan biosynthetic enzyme as shown in SEQ ID NO: 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, and 43, or an enzyme which is at least 95% identical to SEQ ID NO: 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, and 43.

[0011] The inventive method (as well as the products made thereby) thus concern sphingan gums, particularly diutan types, including, without limitation, S88, S60, and S657.

[0012] As noted above, the present invention is the culmination of development and realization that specific DNA sequences that are introduced in multiple copies within certain Sphingomonas strains can provide increased biosynthetic production of high viscosity diutan polysaccharide. The engineered bacteria containing such genes for increased production produce significantly greater amounts of diutan polysaccharide compared to non-engineered bacteria and create the aforementioned resultant high viscosity properties.

[0013] The DNA sequences that are introduced within the host organism (in any well known form, such as, again, as one non-limiting example, a plasmid) to generate the aforementioned increased production and increased viscosity properties (through what is believed, without any reliance upon any specific scientific theory, an increase in molecular weight range properties) according to the present invention may be isolated, recovered and cloned by techniques that are readily available in the art. Thereafter, the DNA is delivered into bacteria of the genus Sphingomonas in multiple copies (via plasmid, other known manner) or increased expression of the genes via a suitable, e.g., stronger promoter. After insertion into the target bacteria, the production of diutan can be determined by fermenting the engineered bacteria and comparing the yield in terms of amount produced and quality produced. Increased production and viscosity increases can both be determined by comparing diutan production via the inventive method in comparison with the wild type diutan-producing strain (ATCC 53159).

BRIEF DESCRIPTION OF THE DRAWINGS

[0014] FIG. 1 is a diagrammatic representation of the isolated genes for diutan gum biosynthesis. Putative or known genes are indicated. The segments inserted into different plasmids are also indicated.

[0015] FIG. 2 is a graphical representation of the improvements in intrinsic viscosity measurements achieved by such inventive diutan biogum materials.

DETAILED DESCRIPTION OF THE INVENTION

[0016] The following terms shall be used throughout the specification in connection with the present invention and have the meaning indicated:

[0017] The term "Sphingomonas" is used throughout the specification to refer to strains of gram-negative bacteria from the genus Sphingomonas.

[0018] The term "increased producer" or "increased production" is used throughout the specification to describe engineered bacteria containing multiple copies of DNA sequences isolated from the same strain which produce significantly greater (at least about 5% more on a weight by weight basis) diutan polysaccharide compared to wild-type bacteria of the same strain.

[0019] The term "isolated" is used to describe DNA which has been removed from a microorganism and subjected to at least some degree of purification, i.e., one or more purification steps, and which can be cleaved or cut by restriction enzymes, cloned into multiple copies or inserted into plasmid vectors or otherwise inserted or incorporated into bacteria.

[0020] The term "sequence" is used to describe a specific segment of DNA which is identified by its nucleotide units. The term "inserted" is used throughout the specification to describe the process and outcome of transferring DNA segments isolated from the chromosomal DNA of a diutan-producing Sphingomonas strain into the Sphingomonas strain (via a plasmid, as one non-limiting example). Such isolated DNA may be introduced first into, again as one non-limiting possibility, the desired plasmid (here pLAFR3), by well-known techniques in the art, and then transferred, for example, by conjugation or mobilization into a recipient Sphingomonas bacterium. After insertion into a recipient Sphingomonas bacterium, the plasmid containing the relevant DNA sequence will replicate in the recipient cell to give several (at least two and usually 4-10) copies of the DNA segment necessary for increased production of high viscosity (again, believed to be high molecular weight range) diutan polysaccharide. The use of conjugation or mobilization to transfer the plasmid vectors into recipient bacteria is generally effective, Electroporation or chemical transformation of competent cells with purified DNA may also be used. Other vectors or bacteriophages can be used to transfer DNA into the host cell. Maintaining the DNA segments on plasmids (or other well known delivery vectors) in the recipient diutan-producing Sphingomonas is not necessary. It is routine to introduce additional copies of a DNA segment into the bacterial chromosome so that the segments are replicated each generation by the same mechanism that replicates the bacterial DNA. Alternatively, increased expression of the genes may be achieved by using stronger promoter elements.

[0021] The term "gene amplification" is used to refer to either increased copies of genes, for example by cloning the target genes on a multicopy plasmid (such as from 4 to 10 copies) or insertion of multiple copies (such as from 4 to 10) of the genes into the bacterial genome, or alternatively increased expression of genes by modification of promoter elements to increase gene expression. Both of these methods and others can result in increased amounts of the encoded proteins.

[0022] The term "biosynthesis" is used throughout the specification to describe the biological production or synthesis of diutan by Sphingomonas bacteria. Diutan polysaccharide is synthesized from individual carbohydrate units in a series of steps controlled by a large number of enzymes of the bacteria.

[0023] The relevant DNA sequence which is incorporated into the recipient bacteria in any selected form (such as, again, preferably, but not necessarily, plasmid form) encodes genetic information which is known to be beneficial or essential for the biosynthesis of increased production and increased molecular weight diutan polysaccharide. Additionally, though, the particular inventive DNA sequence (such as within plasmid pS8) is believed, without relying on a specific scientific theory, to induce, not just increased production, but also an increase in number of repeating units polymerized within the individual polymers of the diutan itself. As a result, it is believed that such an increase in repeating units produces the resultant high viscosity properties surprisingly provided by the diutan gum. A molecular weight increase has been hypothesized due to measured increases in intrinsic viscosity which is related to molecular weight by a power law relationship. For a linear polymer (like diutan gum), intrinsic viscosity is thus known to be essentially proportional to molecular weight in that respect.

[0024] The isolation of the relevant DNA sequences that are the basis of this inventive method and that generate the increased viscosity diutan polysaccharide is accomplished via standard techniques and methods. Such sequences may thus be generated from a diutan-producing Sphingomonas strain that has been cultured using standard procedures. Extraction of the DNA can then be performed, for example, through initial centrifugation and resuspension of the bacterial cells and then subsequent elution of the DNA through purification columns. After purification is completed, the isolated DNA can be digested with restriction endonucleases and cloned into the desired plasmid or other delivery vector and subsequently transferred to a recipient strain. Other techniques as are known in the art can be used without limitation.

[0025] The cloning of DNA in the present invention relies on general techniques and methods which have become standard in the art. It is noted that any number of methods may be used to clone the DNA segments according to the present invention and the present invention is not limited, for example, to the use of plasmid cloning vectors. For example, the DNA fragments may be cloned by insertion into a bacteriophage vector.

[0026] The cloned DNA sequences can be then introduced to a Sphingomonas strain via a plasmid or other delivery vector. The genetically modified Sphingomonas strain can then be used to produce diutan by fermentation. Basically, a suitable medium for fermentation is an aqueous medium which generally contains a source of carbon such as, for example, carbohydrates including glucose, lactose, sucrose, maltose or maltodextrins, a nitrogen source such as, for example, inorganic ammonium, inorganic nitrate, organic amino acids or proteinaceous materials such as hydrolyzed yeast, soy flour or casein, distiller's solubles or corn steep liquor, and inorganic salts. A wide variety of fermentation media will support the production of diutans according to the present invention.

[0027] Carbohydrates can be included in the fermentation broth in varying amounts but usually between about 1 and 10% by weight (preferably 2-8%) of the fermentation medium. The carbohydrates may be added prior to fermentation or alternatively, during fermentation. The amount of nitrogen may range from about 0.01% to about 0.4% by weight of the aqueous medium. A single carbon source or nitrogen source may be used, as well as mixtures of these sources. Among the inorganic salts which find use in fermenting Sphingomonas bacteria are salts which contain sodium, potassium, ammonium, nitrate, calcium, phosphate, sulfate, chloride, carbonate and similar ions. Trace metals such as magnesium, manganese, cobalt, iron, zinc, copper, molybdenum, iodide and borate may also be advantageously included.

[0028] The fermentation can be carried out at temperatures between about 25° and 40° C., with a temperature range of about 27° and 35° C. preferred. The inoculum can be prepared by standard methods of volume scale-up, including shake flask cultures and small-scale submerged stirred fermentation. The medium for preparing the inoculum can be the same as the production medium or can be any one of several standard media well-known in the art, such as Luria broth or YM medium. More than one seed stage may be used to obtain the desired volume for inoculation. Typical inoculation volumes range from about 0.5% to about 10% of the total final fermentation volume.

[0029] The fermentation vessel may contain an agitator to stir the contents. The vessel also may have automatic pH and foaming controls. The production medium can be added to the vessel and sterilized in place by heating. Alternatively, the carbohydrate or carbon source may be sterilized separately before addition. A previously grown seed culture can be added to the cooled medium (generally, at the preferred fermentation temperature of about 27° to about 35° C.) and the stirred culture can be fermented for about 48 to about 110 hours, producing a high viscosity broth. The diutan polysaccharide can be recovered from the broth by the standard method of precipitation with an alcohol, generally isopropanol.

PREFERRED EMBODIMENTS OF THE INVENTION

Including Detailed Descriptions of the Drawings

[0030] The following examples are provided to illustrate the present invention. The description of the examples should not be misconstrued to limit the scope of the present invention in any way.

DNA Sequence Isolation/Plasmid Production

[0031] To undergo the initial isolation and determine the proper sequence for the inventive results described previously, a gene library of the ATCC 53159 organism was constructed as follows: Chromosomal DNA was isolated from Sphingomonas sp. ATCC 53159 and partially digested with Sau3AI restriction endonuclease. DNA fragments in the range of 15 to 50 kb were purified from an agarose gel and ligated into BamHI digested cosmid cloning vector pLAFR3 (in accordance with Staskawicz, et al., "Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syrinaeipv. Glycinea", J. Bacteriology. 1987. 169: 5789-94), isolated from Eschericia coli strain JZ279 (from Harding, et al., "Genetic and physical analysis of a cluster of genes essential for xanthan gum biosynthesis in Xanthomonas campestris", J. Bacteriology. 1987. 169: 2854-61). Ligation reactions were packaged in `A phage particles (using Gigapack III Gold packaging extract, from Stratagene, La Jolla, Calif.) and transfected into Library Efficiency E. coli D-15aMCR cells (Life Technologies, Rockville, Md.). Approximately 10,000 tetracycline resistant colonies were pooled to form the gene library. From this library, individual sequences were then isolated. The work undertaken in this instance involved the isolation of specific genes for polysaccharide biosynthesis from the Sphingomonas ATCC 53159 organism.

[0032] Such genes for polysaccharide biosynthesis are typically identified by complementation of mutants defective in polysaccharide synthesis, particularly those blocked in the first step, glycosyl transferase I. Since initially no transferase I defective mutants of ATCC 53159 were available, complementation of transferase I defective mutants of Sphingomonas elodea and Xanthomonas campestris were utilized to identify genes for diutan polysaccharide biosynthesis. Plasmid pLAFR3 can be transferred from its E. coli host to other gram-negative bacteria by tri-parental conjugation using a helper plasmid that supplies IncP transfer functions (in accordance with Ditta, et al., "Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti", Proc. Natl. Acad. Sci. 1980. 77:7347-51.). RK2 type plasmids have an estimated copy number in E. coli of five to seven per chromosome (Figurski et al., "Suppression of ColE1 replication properties by the Inc P-1 plasmid RK2 in hybrid plasmids constructed in vitro", J. Mol. Biol. 1979 133: 295-318.).

[0033] The gene library of ATCC 53159 chromosomal DNA in E. coli was transferred into a nonmucoid mutant (GPS2) of S. elodea ATCC 31461, by triparental conjugation, selecting for tetracycline and streptomycin resistance. The helper plasmid used was pRK2013 (in E. coli strain JZ279), which contains a narrow-host-range origin of replication but exhibits trans acting functions needed to mobilize pLAFR3. Plasmid pRK2013 was not replicated in Sphingomonas strains. S. elodea ATCC 31461 produces the polysaccharide gellan. Both gellan and diutan polysaccharides have the same tetrasaccharide repeat unit, comprised of [4)-a-L-rhamnose-(l 3)-D-glucose-(14)-D-glucuronic acid-(14)-D-glucose-(1].

[0034] Diutan, however, also includes a side chain comprised of two rhamnose molecules attached to one of the glucose residues, and is modified by acetyl, whereas gellan has no side chain sugars and is modified with acetyl and glyceryl. The mutant GPS2 is defective in the first step of polysaccharide biosynthesis, i.e., transfer of glucose-1-phosphate from UDP-D-glucose to the bactoprenyl phosphate lipid carrier by glucosyl transferase I-enzyme. From tetracycline selection plates, polysaccharide-producing (mucoid) colonies were isolated from a background of non-mucoid colonies. Clones restoring polysaccharide production presumably contained the ATCC 53159 gene encoding glucosyl transferase I plus approximately 20-25 kb of adjacent DNA. Plasmid DNA was isolated from eight mucoid (GPS2 transconjugants and transferred to E. coli strain DH5a (Life Technologies) by electroporation. The plasmids were isolated from E. coli to obtain sufficient DNA for double-digestion with restriction endonucleases HindllU .English Pound.coRI (which cut either side of the BanmHl restriction endonuclease site in the polylinker), to excise the insert DNA from the vector. The sizes of the insert DNA in the clones were determined by gel electrophoresis. The end sequences of several plasmids were determined by sequencing from primers specific to plasmid sequences flanking the BamHI site of the vector. The sequences were analyzed by comparison to sequences in computer databases using BLASTX. Two of these plasmids, pS8 and pS6, are presented in FIG. 1. Similarly, the ATCC 53159 gene library was transferred into a rifampicin-resistant nonmucoid X. campestris mutant defective in transferase I (CXC109) (such as in the Harding et al. reference noted above) through triparental conjugation selecting for resistance to tetracycline and rifampicin. X. campestris produces xanthan polysaccharide, the synthesis of which is also initiated by transfer of glucose-1-phosphate from UDP-D-glucose to the bactoprenyl phosphate lipid carrier by transferase I enzyme (lelpi et al., "Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris", J. Bacteriology. 1993. 175: 2490-500). Plasmids were purified from mucoid transconjugants and the end sequences determined as described above. Two of these plasmids pX6 and pX4 are presented in FIG. 1.

[0035] The S657 DNA cloned in plasmids pS8 and pX6 was completely sequenced by double-stranded shotgun sequencing at Lark Technologies Inc., (Houston, Tex.). These sequences were analyzed to identify the genes for diutan biosynthesis (presented in FIG. 1). Gene functions were designated based on homology to other genes in databases, in particular to the published genes for biosynthesis of S-88 sphingan (such as within the aforementioned '516 Pollock et al. patent), GenBank accession number U51197 and gellan (GenBank AY217008 and AY220099). Genes were identified (FIG. 1) that encoded the transferases for the four sugars of the backbone and four genes for dTDP-rhamnose synthesis. Genes for secretion of the polysaccharide were based on homology to genes for biosynthesis of other polysaccharides. Two genes encode proteins homologous to proteins involved in protein secretion. Two genes putatively encode a polymerase and a lyase. The insert in plasmid pX6 contained 17 genes including gene dpsB encoding transferase I (which initiates the first step in diutan synthesis), genes for secretion and four genes for dTDP-rhamnose synthesis, but lacks the genes for transferases II, III and IV and the putative genes for polymerase and lyase Plasmid pS8 contains 20 genes of the dps gene cluster, including genes for all four backbone sugar transferases, the four genes for dTDP-rhamnose synthesis, and genes for secretion of the polysaccharide, including the putative genes for polymerase and lyase, but lacks the genes of unknown function, orf6 and or/7. Plasmid pS6 contains genes for secretion and the four sugar transferases but does not have all genes for dTDP-rhamnose synthesis or the gene for polymerase. Plasmid pX4 contains only a small part of the dps region but includes the gene encoding transferase I and the four genes for dTDP-rhamnose synthesis that were reported by Pollock et al. to be sufficient to result in an increase in production of polysaccharide in Sphingomonas strains.

Strain Production

[0036] The four plasmids described above were then introduced within Sphingomonas strain ATCC No. 53159 by triparental conjugation as described above to form the novel S657 engineered strains (S657/pS8, S657/pS6, S657/pX6 and S657/pX4. Fermentation was followed, as described above, thereafter in order to produce a biogum material as noted below. All four plasmids had a beneficial effect on diutan productivity; however, the pS8 plasmid surprisingly also provided extremely large increases in diutan viscosity, and increase in molecular weight. The DNA sequence ofpS8 (26278 bps)(DNA Sequence No. 1) is provided and the encoded genes are listed in Table I below, and in diagram form in FIG. 1. The insert DNA in plasmid pS8 includes genes dpsG through rmlD and a portion of genes dpsS and or/7.

[0037] The following gene table is basically a list of the genes represented by the DNA sequence for insert in plasmid pS8 as provided within FIG. 1

TABLE-US-00001 TABLE 1 Genes on pS8 plasinid insert Start End Name Description 2* 1054 dpsS (partial) homologous to gelS 2738 1113 C dpsG putative polymerase 4895 2898 C dpsR putative lyase 5093 6031 dpsQ putative rhamnosyl transferase IV 7082 6111 C dpsi unknown 7121 8167 dpsK beta-1,4-glucuronosyl transferase II 8164 9030 dpsL glucosyl transferase III 10467 9079 C dpsJ unknown 11076 17374 dpsF unknown 12389 13306 dpsD putative polysaccharide export protein 13341 14687 dpsC putative polysaccharide export protein 14687 15394 dpsE putative polysaccharide export protein 15405 16286 dpsM putative polysaccharide export protein 16270 16968 dpsN putative polysaccharide export protein 18454 17060 C atrD putative secretion protein 20637 18451 C atrB putative secretion protein 21229 22641 dpsB glucosyl-isoprenylphosphate transferase I 22757 73615 rmlA glucose-1-phosphate thymidylyltransferase 23632 24198 rmlC dTDP-6-deoxy-D-glucose-3-5-epimerase 24202 25263 rmlB dTDP-D-glucose-4,6-dehydratase 25263 26129 rmlD dTDP-6-deoxy-L-mannose-dehydrogenase 26277* 26146 C orf7 (partial) unknown function *First in-frame codon, the start codon is not present

Diutan Production

[0038] Diutan production by the engineered plasmid-containing Sphingomonas S657 strains compared to the S657 wild-type strain without a plasmid was determined in three sets of fermentations run in the same liquid media in Applikon 20L fermentors, with agitation and aeration. For the plasmid containing strains, the antibiotic tetracycline at 5 mg/L was added throughout the fermentation to ensure retention of the plasmid. KOH was added as needed to control pH. Two seed stages were used with 1% to 6% inoculum transfers. Media used for fermentation contained corn syrup as carbohydrate source, an assimilable nitrogen source and salts. Nutrients that can be used for fermentation are well known in the art and include a carbohydrate, for example, glucose, sucrose, maltose or maltodextrins, a nitrogen source, for example inorganic nitrogen as ammonium or nitrate, organic nitrogen such as amino acids, hydrolyzed yeast extract, soy protein, or corn steep liquor, and additional salts containing for example, chloride, phosphate, sulfate, calcium, copper, iron, magnesium, potassium, sodium, or zinc.

[0039] As a measure of the resultant diutan production, broth viscosity and precipitated fibers were determined. The viscosity of the fermentation broths was measured via a Brookfield viscometer run at 60 rpm with a spindle #4, and the results are shown in Table 2. At the end of the fermentation, the broths were treated with the well known introduction of glucoamylase enzyme to hydrolyze any remaining oligosaccharides from the corn syrup. The diutan gums produced were then precipitated from an aliquot of broth with two volumes of isopropyl alcohol. The fibers were collected on a filter and dried. In Table 2, the term DWY means the total precipitable dry weight yields of biogums after hydrolysis of excess oligosaccharides from corn syrups

[0040] Clearly the resultant material is in higher yield with plasmids pX4, pX6, pS6 or pS8 carrying additional copies of genes for diutan biosynthesis present therein. However, with the pS8 plasmid, there was an unexpected high increase in broth viscosity relative to the increase in dry weight yield indicating that some factor in addition to increased amount of diutan produced was affecting the viscosity.

TABLE-US-00002 TABLE 2 Fermentation of plasmid-containing strains Strain Run#1 Run#2 Run#3 av. % Increase DWY S657 34.3 32.2 33.9 33.5 -- S657/pS8 37.1 35.4 35.9 36.1 8.0% S657/pX6 38.4 37.6 33.5 36.5 9.1% S657/pS6 37.6 12.3% S657/pX4 36.4 8.8% Broth Viscosity S657 5150 4950 5550 5217 -- S657/pS8 6650 6850 6850 6783 30.0% S657/pX6 5400 6250 5125 5592 7.2% S657/pS6 6675 28.0% S657/pX4 5525 5.9

[0041] Clearly, there was a higher yield of resultant material with any of the four plasmids present therein, whereas the pS8 and pS6 plasmids permitted a highly unexpected increase in broth viscosity thus indicating high product quality as well. The quality, i.e. viscosity, of the resultant diutan gum products was then determined.

Diutan Rheology in Applications Tests

[0042] These diutan gum samples were then analyzed in terms of potential beneficial uses within two different areas: oilfield additives for oil recovery and cement additives for water retention and quick set-up.

[0043] The oilfield industry relies upon what is termed a "sea water viscosity" (SWV) test as an estimate of acceptable performance for gums for oil recovery. Such a test basically is an indicator of the effectiveness of a gum to increase viscosity in briny conditions of water (to replicate recovery from seabeds, for example).

[0044] The prediction of the viability of a resultant gum as a proper viscosity modifier for oil recovery purposes is generally accepted in terms of viscosity modification of a test sea water formulation. Such a "Synthetic Seawater" formulation is produced by mixing 419.53 grams of Sea Salt (ASTM D-1141-52) in 9800 grams deionized water. For the seawater viscosity test, 0.86 grams of the sample gum is added to 307.0 g Synthetic Seawater and mixed at approximately 11,500 rpm in a Fann Multimixer (Model 9B5, part number N5020) for 35 minutes. At the end of 35 minutes, the solution is cooled to approximately 26° C. before the viscosity is measured. For the 3-rpm reading, the sample is placed on the Fann sample platform (Fann model 35A; Torsion spring MOC 34/35 F0.2b; Bob BI; Rotor R1) and the speed is adjusted to 3 rpm by turning the motor to low speed and setting the gearshift in the middle position. The reading is then allowed to stabilize and the shear stress value is read from the dial and recorded as the SWV 3 rpm dial reading (DR). For the 0.3-rpm reading, a Brookfield viscometer is used (Brookfield LV DV-II or DV-II viscometer, with LV-2C spindle) to measure the viscosity. The speed of the spindle is set to 0.3 rpm and the spindle is allowed to rotate at least 6 minutes before the viscosity is recorded as the SWV-0.3 rpm reading and expressed in centipoises (cP). For cement applications, the PEG LSRV test (a low shear rate viscosity using polyethylene glycol as dispersant as outlined below) provides an indication as to effectiveness of performance of a viscosity modifier to that industry. Such a test measures the viscosity of a 0.25% solution of biogum in Standard Tap Water (STW). STW is prepared by adding 10.0 grams NaCl and 1.47 grams CaC3/423/40 to 10 liters deionized water. For the viscosity measurement, 0.75 grams of biogum is added to 4.5 grams Polyethylene Glycol 200 (CAS 25322-68-3) in a 400-mL beaker and thoroughly dispersed. Then, 299 grams of STW are added to the beaker and mixed for approximately 4 hours using a low-pitched, propeller-style stirrer at 800±20 rpm. After the 4-hr mixing time, the beaker is placed in a 25° C. water bath and allowed to sit undisturbed for approximately 30 minutes. The viscosity is then measured using a Brookfield LV viscometer equipped with a 2.5+ torque spring (or equivalent instrument such as Model DVE 2.5+) at 3 rpm using the LV 1 spindle after allowing the spindle to rotate for 3 minutes and expressed in centipoises (cP).

[0045] The diutan samples produced above were tested in this manner; the results were as follows:

TABLE-US-00003 TABLE 3 Rheology of diutan from plasmid-containing strains SWV3 rpm SWV-0.3 rpm PEGLSRV Strain Run#1 Run#2 Run#3 Run#1 Run#2 Run#1 Run#2 Run#3 S657 wild-type 25 26 22 24400 28600 2820 3150 2280 S657/pS8 42 43 47 41500 38800 4720 4980 4920 S657/pX6 25 29 26 25000 29100 2860 3400 3270 S657/pS6 -- -- 22 -- -- -- -- 2770 S657/pX4 -- -- 24.5 -- -- -- -- 2950 SWV = v1scos1ty m sea water LSRV = low shear rate viscosity

[0046] Unexpectedly, there are definite increases in viscosity exhibited by the inventive diutan gums produced by some of the engineered plasmid-containing strains Most surprisingly, however, is that the increase in viscosity for SWV at 3 rpm for the pS8 strain is 80%, whereas the same analysis made for the pX6 strain is merely 9.6% over the wild-type results. Plasmids pS6 and pX4 had no significant increase. Likewise, the lower SWV rpm test reveals an increase of 51.5% over the wild-type for the pS8 type versus just over 2% for the pX6. Finally, the polyethylene glycol LSRV test showed that the pS8 results were in excess of 77% viscosity increase over the wild-type gum, as compared with less than 16% increase for the pX6 diutan, and 7.2% increase for pX4 and no significant increase for plasmid pS6. Again, the highly unexpected results in these terms shows the drastic improvements accorded diutan gum production via the utilization of the needed gene sequence exemplified within the pS8 plasmid, as one manner of introducing such a sequence within a target diutan-producing bacterium.

[0047] Thus, the inventive diutan produced via the introduction of pS8 exhibited surprisingly increased viscosity measurements on all three counts, particularly as compared with the wild type and pX6 plasmid-produced varieties. Thus, it was expected that such a novel diutan would function extremely well under typical oilfield conditions and within cement applications.

Fundamental Explanation for Rheology Improvement

[0048] The previous examples showed that diutan from the S657/pS8 strain showed a significant increase in rheological parameters. Such a substantial increase in the sea water and PEG low shear rate viscosity measurements thus cannot be attributed to the increase in productivity alone since the pX6 strain also exhibited similar, if not greater, yield results. Indeed, in the prior example illustrated by Table 2, the dry weight yields (alcohol precipitable matter) increased by 8.0%, while the rheological parameters

[0049] increased significantly more for the S657/pS8 strain (52-80%). A fundamental study was pursued to explain why rheological improvements are obtained with strain S657/pS8 over the wild-type strain.

[0050] Intrinsic viscosity is a well known technique in polymer science to infer the molecular weight of macromolecules (C. Tanford, 1961. Physical Chemistry of Macromolecules, John Wiley & Sons, New York). The intrinsic viscosity is obtained by plotting the reduced viscosity (viscosity normalized for concentration) versus the solution concentration, and extrapolating a linear regression of the data to zero concentration (the

[0051] y-intercept of the plot). Surprisingly, the resultant gums exhibited increases in intrinsic viscosity as noted below in the following table.

[0052] Five diutan samples, two from the wild-type strain (Control 1, Control 2) and three from the S657/pS8 strain (Sample 1, Sample 2, Sample 3) were evaluated for intrinsic viscosity, neutral sugars, and organic acid analyses. These samples were purified by alcohol precipitation, re-hydrated, treated with hypochlorite, treated with glucoamylase, treated with lysozyme, and finally treated with protease (in that sequential order). They were then recovered at a 4:1 CBM:Broth ratio, dried and milled. CBM is an azeotropic isopropyl alcohol/water mixture including -82% by weight of the isopropyl alcohol.

[0053] The samples were tested for moisture content by performing the following: generally, two 0.7 gram aliquots of sample were tested using a Mettler HB 43 halogen moisture balance. The results from the two trials were then averaged and these results were utilized for moisture correction.

[0054] After obtaining the moisture data, a 0.2% solution of the gum was prepared in 0.01M NaCl on a moisture corrected basis. For these trials 200 grams total of the 0.2% solution were prepared. The gum was weighed on an analytical balance to the nearest ten thousandth and added to the water weighed to the nearest thousandth. The samples were stirred for two hours using a 2.5 inch diameter propeller mixer 1000 rpm in a 400 ml tall form beaker.

[0055] Following initial hydration, each sample was diluted to 0.02% using 0.01 M NaCl. This was done by weighing 20 grams of the 0.2% solution into a 400 ml beaker, then adding back 180 mis of the diluent. The diluted samples were mixed for an additional 30 minutes. The final dilutions ultimately used for determining the intrinsic viscosity were prepared from this sample. Each diutan sample was evaluated at the following concentrations: 0.004%, 0.008%, 0.010%, and 0.012%.

[0056] Viscosity measurements were carried out using the Vilastic® VE System. Prior to measurements the Vilastic was calibrated with water to less than 2.0% error. The samples were measured using the Timer program @2 Hz, a strain of I and a shear rate of approximately 12 l/sec, all at a constant temperature of 23° C. Five measurements were made for each sample and averaged. The averaged viscosity data were then used to calculate the intrinsic viscosity. FIG. 2 and Table 4 below provide the final results of these trials.

TABLE-US-00004 TABLE 4 Comparison of Diutan Based on intrinsic Viscosity Calculations Diutan Measured Intrinsic Sample Solids Viscosity S657 Control 1 93.76 138.3 S657 Control 2 92.42 143 S657/pS8 Sample 1 91.7 170.7 S657/pS8 Sample 2 91.4 162.2 S657/pS8 Sample 3 91.94 162.8

[0057] These results indicate that the S657/pS8 strain consistently produced diutan with significantly higher intrinsic viscosity; in fact the average reduced viscosity for the inventive strains was 165.2, whereas the control was 140.7, all at similar measured solids levels. This finding indicates that diutan produced by S657/pS8 is higher in molecular weight than the wild-type control.

[0058] FIG. 2 is the graphical representation of these trends showing the consistent higher intrinsic viscosity measured at similar solids content between the control and inventive strains.

[0059] To determine if the higher viscosity diutan gum from S657/pS8 had the same composition as diutan from the wild-type strain, the composition was determined by testing for neutral sugars and organic acids. The purified sample used for intrinsic viscosity measurements were used for neutral sugar analysis. An aliquot of each purified sample was hydrolyzed to component sugars by hydrolysis with trifluoroacetic acid (100° C./-18 hr). The hydrolysate neutral sugars were quantified by high-performance anion-exchange chromatography with pulsed amperometric detection. The hydrolysate organic acids were quantified by high-performance ion-exclusion chromatography with chemically suppressed conductivity detection. Table 5 summarizes the results from the neutral sugar analysis. As shown, the neutral sugar profile for the S657/pS8 strain is nearly identical with the neutral sugar profile for the S657 wild-type strain. Although both results are different from the theoretical values, these results indicate that the structure of the repeat unit of the diutan gum produced using pS8 is the same as that for wild-type and that any increase in viscosity imparted by the pS8 material is due to longer chains, meaning higher molecular weight.

TABLE-US-00005 TABLE 5 Neutral sugars and organic acid analysis for pSB and wildtype (control) diutan strains Strain % Rhamnose % Glucose % Acetate Sample 1 S657/pS8 32 19 8.9 Sample 2 S657/pS8 32 19 8.2 Sample 3 S657/pS8 32 17 8.6 Control 1 S657 wildtype 30 18 8.6 Control 1 S657 wildtype 33 20 8.7 AVERAGE S657/pS8 32 18.3 8.6 AVERAGE S657 wildtype 31.5 19 8.65 THEORETICAL -- 46 30 8

[0060] The greatly improved seawater viscosity and PEG low shear rate viscosity of the diutan produced by the S657/pS8 engineered strain is thus attributable to an increase in molecular weight or length of the diutan molecule, i.e., more repeat units per molecule

[0061] and not to a change in its composition and thus not to changes in the repeat structure itself. Nor can this improved rheology be due soley to increase in amount of diutan produced. Although four plasmids, pS6, pS8, pX4, and pX6, with different portions of the cluster of genes for diutan biosynthesis cloned, were evaluated, and all showed some increase in productivity, only plasmid pS8 showed the unexpected and very high increase in rheological parameters of the recovered diutan product.

[0062] A comparison of the genes for diutan biosynthesis cloned in the tested plasmids suggests that the most likely gene to be responsible for the increase in molecular weight is the gene dpsG, since this gene is present in pS8 and not in the other plasmids. Gene dpsG encodes a hydrophobic membrane protein with strong homology to other membrane proteins involved in polysaccharide synthesis. A portion of the protein has homology to proteins for polymerase, an enzyme which catalyzes the linkage of repeat units to form the high molecular weight polysaccharide. The homologous gene ge!G in S60 has been postulated to function as a polymerase for gellan synthesis (Harding, N. E. et al. 2004. "Organization of genes required for gellan polysaccharide biosynthesis in Sphingomonas elodea ATCC31461". J. Ind. Microbiol. Biotech. 31:70-82. Sa-Correia, I. et al. 2002. "Gellan gum biosynthesis in Sphingomonas paucimobilis ATCC 31461: Genes, enzymes and exopolysaccharide production engineering". J. Ind. Microbiol. Biotechnol. 29: 170-176). Homologues of dpsG have also been isolated from Sphingomonas strains ATCC 31554 and ATCC 21423 producing polysaccharides S88 and S7 (Pollock et al. U.S. Pat. Nos. 5,854,034, 5,985,623 and 6,284,516, and Pollock, T. J. U.S. Pat. No. 6,709,845). It is thus very likely that additional copies of the gene for polymerase may have an effect on increasing the molecular length of the diutan molecule. It cannot be ruled out that other genes in the diutan biosynthetic gene cluster may be required in combination with dpsG to achieve the observed increase in viscosity. Likely candidates would be the genes dpsB, dpsL, dpsK and dpsQ encoding the sugar transferases I, II, III, and IV, in particular the gene dpsB which encodes transferase I that adds the first sugar of the repeat unit to the lipid carrier. Other important genes may be dpsD, dpsC and dpsE, which are homologous to genes gumB and gumC that have been shown to increase the molecular weight of xanthan when amplified on a multicopy plasmid. It is possible that all genes cloned in plasmid pS8 may be required to achieve the dramatic increase in viscosity.

[0063] While the invention will be described and disclosed in connection with certain preferred embodiments and practices, it is in no way intended to limit the invention to those specific embodiments, rather it is intended to cover structural equivalents and all alternative embodiments and modifications as may be defined by the scope of the appended claims and equivalence thereto.

Deposits

[0064] The following bacterial strain was deposited with the Patent Depository at the American Type Culture Collection at 10801 University Boulevard, Manassas, Va. 20110, on Oct. 21, 2005, pursuant to the Budapest Treaty for the International Recognition of the Deposit of Microorganisms:

[0065] Sphingomonas strain S657 with plasmid pS8.

Sequence CWU 1

1

45126278DNASphingomonas sp. ATCC53159 1gatcaacggc gccttgctcg gacggcacaa attcgtcctg gtcaatgtgt ccacggtcgc 60ctcttcgata ctgttccagc tgttcccgct tgtcgtcgcc tggatgatcg gcccggacct 120gcgaacgctg ctgatcgccg cgctcgttgg ccgggcggtt ccgatgatcg gcatgctgcc 180cgcgctgtat cgaaaccttt tgcgcggcaa cacgccgcgt tttcacgcca gcgaggcgcg 240cttcctgata ggctatggcg ggtgggcctc gctcacgacc gtggtagcga ccgtgctcat 300gatggcggac cgcttcctga ttggcgcact tcttgggccc gtcgccgtga ccatctacac 360ggcccccctg caactcgcac agcgcgtatc gctgctgccc tccgcactgt ccgccgcgct 420gttcccgcgc ctgcccagcg cgacgccggc ggagcgcatg gcgcttcaga tccgctcgct 480gtcgctgatc atgggcggcc ttaccgggat gatcggcggc ggactattgc tggccgcgcc 540gtttctcgat ctctggatcg gcaagtcgct cggccatgcg ggaacgccgg tcgcgctctt 600cctgttcttc ggcgcatggt ggaatgcgct ggcgatcatt tcgttcagcg gcctgcaggc 660gagcggacgg ccgaaagcga gcgcgatcgt ccagggggca gagctgctac ccgtgttgat 720cgcgctgtat gcagggatcc gatggggcgg cgtgaccggc gccgcagcgg tctttctggg 780acgctccgcc ctggatttcg tcctgttgac ctggcaggca ggcctgctcc gccagacggt 840gaagcaagta tccgtatgcg gcgccgttct caccgtcgcg atgctcgtgg gcgcgaccta 900tcgctattcg gtgccgctct ggtgcgtact cagcgcctgc tgcctggtcg cgctggcagc 960ctgctcctgg tggacattgg cgcgccagga caaggcactg ctgattggac gattgagccg 1020aattctacca aagcagcggc aactcgacct atagcctttc cgcaatgcac cgatggacca 1080caccaacccg ttttaattga cacacacaaa tgctacaccg acaaagacac aggccgagag 1140cgatatagaa gcgctatgcc tagccccagc gtcataaaga tgaacgggtc attgtcacct 1200tgcgacagga ctgaccgcgt atttaaaaga acagccagga aagttgctac ggcgagctca 1260agcgggtagc catctccgct catcttaaga ccacgaaacg cgagcaaaat cattaacgta 1320atcatcgtgc cgtatagcga aacaaaaccc agcaagccgt aatcagccgc tacggacagg 1380aaaccactgt cgatcgatag gaagccttgc tgattacgcc acccgacagc gccagcaccc 1440tctcccgggc catagccgaa gaaagggcgg cgagcgatgg caggcacgcc caagcgaaac 1500tgctcctgcc tgccttgatt gctaagttga gaagcgcctc caccgagaac acggttgtgg 1560acggcaggca cgaacatgac cgccagcgac agcgccacca tcaaggcggg atacgtcaac 1620gtcagcgaaa tgccgacaag cccgcccttt gtggtccgcc accgccgaat tgcccaaata 1680agcaaataca cggtatgcgc caccaatccc cccaccattg ccagtcgaga accgctaaga 1740aatccggacg caactacaag aaaatcgaag aaaatccaaa atgccaatct ccctacgcca 1800cgggaattcg ctatacggtg cagcacgaaa ggaatcgtca aagccgtcaa ctctccccag 1860acaagcggac tgctgaaagt cgtcaaaacg cggtaagtac cccggaaacc gggcgtaagc 1920actacggtaa gaaactgctc atcaacgcgc aggaagctcg gaatcgagta ggcccagagg 1980acgtgcttca cccggaactc cagcacgcca atcgccatca gcacgcccac gcaccaaaac 2040aagcgcgtaa cccaccactc cggggtgcgc gtgtcggtcc cgatcagcca tagcgagatg 2100aatgccatcg gcgtcaccgt cagaacgatg ccaatcaacc gcggaattgt ttgcgaggcc 2160gctggggtcg caatggaggc gacgatctgg accataatga aggcaagcaa tagtcgcgat 2220gggatcggcg ccgcccgcat aatcgccgcc atctcggatc gaaacttttt cgagaccgaa 2280agcgagatca tgagcgtgag caatgcgatc gaaccgatca tccgcctgat cgagatccaa 2340ggcaaaccac caacgctgag cgcaagatag ttcggccaca cgagcgccgc caccatatag 2400gcgaggtata gttttgccag caggcgagta ggcgcctgcc gcgcctcggg tagcgcccag 2460atcactacga gcgccatcag aacgaggggc acggccggga tcgccagcat ctggagcggc 2520agaactgcgg cgagcaggcc gtagactgcg gcaagaaaca tcacgctgac cagcagaacg 2580gtacgccgcg ccgcgatcgt cacgcctgat cgctcggctt tgtagacggg cagtaccggg 2640atcgctggct ttgtcagaaa ccgaaccagt cgcaacctgc gaagccgctg catcgctccg 2700tggaaggccg ggcgacgaaa cgccgaggta gtcgtcatct gcaagtcccc aacaagtccc 2760caagaggcgc tgccgctcgc atgatcgaag ggttcgcgaa aagcaaggtc gatacgccgc 2820actccctgcg atgtgccgcc ggatcgcagg agggcacggg cggcgccggc gcaaggccgc 2880tcaccgcccg cccccgctca ggcgcggtac aggttgtact gatccgccgt agcgctcagt 2940gtcgccgcgc tgcggattgc gcccatcgcc cccgcggtca tcatgtcgac accgatcttg 3000ctgacgagcg cgatctgcga ggacgcggca gtacctatag acagcgtact gcccaccgtg 3060gccaccgtcg caagcggcgt tgccgtgcta gcggcgccgg cacccgccag cagcgcagcg 3120gcctgcgcgg ccgcgccggt gacgaggctg tccttgaccg tcgccgccgc gctggcgctc 3180gacgcggtca ccagcgcctg cacctgggcg gcgctgatcg cgccatcgcg gatctcgatg 3240tcgccgaccg ttccgctgaa tgcggtcgag aacgggctgc cgacatacag cccccaggat 3300tcggcgggcc gggtcgtgcc ggtcatcgtc gctgtgccgc gttgcatgcc gtctacgtac 3360agaatcgcgg ttttccgcgt gctgtcgtag gtcagggcga tcttgtgtgt ggcagcatca 3420agcagcttgg cgccgctcgt caccattgtc tggctgacgc ccgcggcgtt gcgcatggtg 3480aagctcagtt ccccattggc ctgcagcgaa accgaccagc tctggaagat gccaagaatt 3540tgcccggccg tggccgtagc cgagtcccgc ttgaggtcga agctgagcgt gaacgccgac 3600aatgcgtaaa tctgccgcga atagctccgg tttagttcca cccccgtgcc cgtcgagacg 3660tggaaggcgc tgcccacgac cgccgacacg tccaccgcct ttgtcgtctg gccggtattc 3720cagtgcgaaa ggtccacgac gccgctgttg ctgaacgaca gatcgagcag cagcgacgga 3780tttgccgcct tcgcagtcga cagttcggta gtcacctgag cggcagcagc gctcgacacg 3840ggcggctggt acccgacgcc gggaacgatc aaatcgctga gccgcgccgt agccccatcg 3900ttgaggccat agatcttgcg gatcgttgcc gagtcactcg tcagcgtacg attgcctgtc 3960tgcacgatat tgctcgagga gcttgtgacg gtgatcaggt ccgcaacatt gttcttgatc 4020gtcgcgccat tggttttgtc gaggcgaatc caaaatgatg tgccatccac ttgcgatatc 4080acgctattgg attcgatatt gacattaacg ccgttaacaa cgttgatacc gtggtaataa 4140ccattcagat agataagatt gtttttgatg tttacattga catagggaag attaccggcc 4200tcgtcattca tgaaaatccc ttgcgcgcca gagcccgcgc cctgcatgat gacgttattg 4260gagatggtga tgttggtatt gcccttgacc ttgcccgccg tgaagaactg aatggcgtcg 4320ggatgttcgg tgcccacggg aaacaggttc gtgaacgaat ttccgtcgat gacaagattg 4380ttcatctcag tgaagttcgt atgatcgcgc cggttgtcgt ggaagctgct gttctggacc 4440accatgccat cgacgttgta ggcctcaagg cccagaccga agtggtcgat agacgaattc 4500tgcatcgtca ccgacgtgct gttgcgcacg aacaagcccg cccccttcga gagcgaaggg 4560tcaccagtgc cgccgctgaa ccgcacgccg tccaaaacga tgttggccga accctggatc 4620gtattcagtc gattccagtc atcggcgggc ttgtaatcgg tcgcagcgac catgtttttg 4680acggtaacgt tgctactgtt cccgatcacc agcttttgga tattgaccgg gttcgacgag 4740tcgagcgact caattgtcac catgctggta aacgtcttgg tcattacagt gagatctgtg 4800tagaccccgg cggcaagctt gatggtttcg ccacccttcg ccgccgcgat tgcagcattc 4860aactccgtct gattcttgac aatgatatcc ggcatgttga cttaccccgt acgcacgaac 4920ccgggccgat attgaccctt ccattgtcat aaataccaga acagccatga aatttgctcg 4980aagggataca gttaagaact cccttctacg gggccgcatg ccgggcccat gcacgcccga 5040ctttcgccgg caccgtctcg acggcgcaac acagtgcagc tactagggtg cgatgcagat 5100gctcccaacg cccgatgtca gcatactcgt ggtcgctttc aactcgaccg agtatatcga 5160agactgcctg cgcggcatcg ccgaaggagc gggcaagacc ccccacgaag ttctgctgat 5220cgacaatggc gacgggcgaa ccgaagcgct ggtccggcag cggttccacc acgtccgcat 5280cgttcccagt gagggcaata ttggtttcgg ggccggcaat aatcgcctgg cagcgcaggc 5340tgccggcccg ctcctgctgc tcgtcaaccc cgatgccatt ccccagcccg gcgcaatcga 5400tcagttggtc acctttgcca aacagcatcc cgaggcggcg gcatggggcg gccgttccta 5460ctcgcccagc ggcgatctag aacccgcaaa tttcatgtcc ctgccgacgc ccgccgactt 5520tctgacggcg attttcaacg cgcgtgcgct acgcagcggc gggctgcaag aaggcgcgac 5580cacccccgga gcggtcgagg tgttgaatgg cggcttcatg atggtacgca ccgatgtctg 5640gcaggcgatc ggcggttttg acgagagctt ttttctttat tcggaagaga tcgatctctt 5700ccagcgaatc cgcacgttgg ggcacaaggt gctcgtcgac ccctcggtca aagtggtaca 5760caatacgggg agtggtcagt cgatgtccca gaaccgcctg atgtatctca cgaccgggcg 5820catgcactat gcgcgaaagc attttggcgc actcggcacc cttgccaccg ggtgcgcgct 5880ttggctgatc gccgccaaat acacgttggt cggggcggca ctctggcgcc tgtcgccgcg 5940gacgggcacg cgatacaaag agctgagcaa cgggtggcgt gccgtattta gcaatcctgg 6000ccgatggtgg agcggctatc cgcgtcgcta aaagtccagc tccccccccc ctaaaggcgc 6060cgttgggagg cggacgcatc gttgcaacaa cgcgcccgcc tttcagacct tcagttcccc 6120gccggcgttg cgccgctgcc gcgaagctgc ggcggtgcgc tgtagccggc ctgatatttc 6180acggtttccc gcgccttctt caggcggtcg ttgagctgtg cgtcagccgc cttgccgaag 6240cgctcggtac gcagcccgct gagcgcgatc tcgcgcgcct ggtcggccgg caccggcagc 6300accgtggtcg acgtgatgat attcgcggtc agtccctgct gggtcggcag gatgaacatc 6360tcctgtgccg gcagcgacgc gatcttggca gcgatttccg gcggcagcgc agcggtgtcg 6420atctgcgacg gcgcgcgacg gaactggaca ttgtccgccg agagcttggc ggttagctgg 6480tccagcgtct tcagcggcgc gaattgcttg agctttgcgg ccgagctcgg cggagcgaag 6540acgacctgat cgatcgcgta gatcttgcgc tgcgcgaacc gctccggatg cgcggcctga 6600tatttctcga tctcggcatc ggtcggctgg gcgatgccgc cggcgatctt gtcgcgcagc 6660atggcggtga ggatcagctc gtcggcccgg cgctcctgga tcaggaaggc aggcgtcttg 6720tccagcttct gctcgcgggc gaccttggcg aggatcttgc gctcgatgat gcgctgcagc 6780gccagctgct cggccagctt gcgatcggtc cccgggggta cctgggaggc ctgcagttcg 6840gcattcagct cgaagacggt gatttcttcg ccatcgacgc tggcgaccac ctgccccttg 6900tcgagcttgc cgcccttgcc gccacatccg gagacggcca gcgcggccgc agccaccgcc 6960gtaaccaggt acaatttctt catgaagacc tccccgccgg cacggaattg cgcacggcac 7020aaacttctac ttgaacctat tcggacgggc gggcatccgc aatagcgttg gcagtgcagc 7080atggttctaa gcggagccag gcggcaacaa gggggacgag atggcagaag cgaacgcggt 7140agatggaaag gcctccaagc cgctgaaaat gtgccttgca gcgtcgggcg gcggccatct 7200ccggcaaatc ctcgatctgg aatcggtgtg gcgcgaacac gattatttct tcgttactga 7260agataccgcg ctcggccgga gccttgccga aaaacatccc gtcgaactgg tggagcacta 7320tgcgctcggc caggccaagc tgggccatcc cttgcgcatg ctgggcggcg catggcgcaa 7380cctgcgccag agcctttcga tcctgcgccg gcacaagccg gatgtggtga tttccaccgg 7440cgcgggcgca gtctatttca ccgcgctgct cgccaaactg tcgggcgcca agttcgtcca 7500tatcgaaagc ttcgcgcgct tcgaccaccc gtctgccttc ggcaagatgg tgaagggcat 7560cgcgacggtg acgatcgtcc agtcggcggc gctgaaagaa acctggcctg atgccgagct 7620gttcgatccg ttccgcctgc tcgatacacc gcgcccgccc aagcaggcgc taatcttcgc 7680gacggtcggc gccaccctgc ccttcccgcg gctggtgcag gcagtgctcg acctgaagcg 7740cgccggcggg ctgccgggca agctgatcct gcaatatggc gaccaggacc tgcccgatcc 7800cggcatcccc gacgtcgaga tccgccgtac catcccgttc gacgatctgc agctgctgct 7860gcgcgatgcg gatatggtga tatgccacgg cggcaccgga tcgctggtca cggcgctgcg 7920cgccggctgc cgggtcgtcg cctttccgcg ccgccacgat ctgggcgagc attatgacga 7980tcaccaggaa gagatcgccc agaccttcgc cgaccggggc ctgctccagg cggtgcgcga 8040cgagcgccag ctcggcgccg ctgtggaagc ggccaaggca accgagccgc agctggcgac 8100caccgaccac acggccctcg cggcgcggct gcgccagctg ctggcgcagt ggagtgccaa 8160gcgatgagca cgccccggat cagcgtcgtc atcccgcact ataacgatcc gcaatccttg 8220cggctctgcc tggatgcgct ggagcggcag acgatcggtc gcgacgcgtt cgagatcatc 8280gtcggcgaca acaattcgcc ctgtgggctc gcggcggtgg aggcggcggt cgccggacgt 8340gcgcggatcg tgaccattct ggaaaagggg gcgggccccg cgcgcaacgg ggcggcagcc 8400gcagcgcgtg gcgagatcct cgcctttacc gacagtgact gcgtggtgga gcccggctgg 8460ctggcgggcg gcacgaccag ggtcgcgcct ggccgtttca tcggcgggca catgtatgtg 8520cgcaagcccg aagggccgcc gaacggcgcc gaggcgctgg agatggcgct ggcgttcgac 8580aatgaaggct atgtgcggcg cacccagttc acggtcaccg caaacctgtt cgtgatgcgc 8640gccgatttcg aacgggtcgg cggcttccgc gttggcgtgt ccgaggatct ggaatggtgc 8700caccgggcga tcgccagcgg cctcaccatc aactatgcac cggatgcatc ggtgggccac 8760ccgccccggc ccgactggtc ggccctgctg gtgaagacgc ggcgcatcca gcgcgaactc 8820tatctgttca acatcgagcg gccgaagggc aggctgcgct ggctggtccg ttccgtggcg 8880caaccggcga tgatcccaca ggacgtggcc aagatcctgc gcacaccggg taccaagggc 8940gcgcgcctcg ctgcggtcac cacgctggtc cggctgcggc tgtggcgcgg cggcgccggc 9000ttgttgcagt tgctcggccg cgacatctga tcgaccggcg atcggccgac gagcgcgtcg 9060ccggccgatc gcattgcatc agacggtggc cagcgcgtct tccagcgtgc cgctgtcgag 9120ccgcaggcgg ccgatcatca gccacagata gaccggcagc gtatcgtcgg tgaagcggaa 9180gcggcaatcg ccgtcctgcg tttcggattc gaggccgagt tgaccggtga gctcgcccag 9240ctcctgctcg acctgcgccg ccgtgatgtg cgcgcccggc agcagatcca ccacggcttg 9300gccgctgaac cagccatccg ccgagcgcga ggcctcgccc agcgccgcga cgagtggatc 9360gtagcggccg ccgacgaact tgcgcatctc gatcaccgcg cgcggcggca tgcggccctc 9420gatctcaagg atcgcctggt cgagcgcacg acgcagatgc ccggcgtcga ccgtgaggcg 9480gccctggtcc agggcttcca gcgcggaatg gtggcacagc agccgcgcga aatagggcga 9540ccccagcgcg agcaggtgga tcatgtgagt caggtccgga tcgaagcgaa cgcccgaggc 9600ggtttcgccg agcgcgatca tctcctgcac ctccgattcc tccagccggg gcatcggcag 9660gccgatgacg ttgcggcgga tcgacggcgc ataaccgatc agctcctgca ggttcgaggc 9720gacgcccgcg atcaccagct ggacgcgcgc cgaacggtcc gacaggttct tgatcagctc 9780ggcgacctgc tgacggaagg cggaatcgct gacgcgatca tattcgtcga ggatgatcag 9840cacgcgtgtg cccgtgatgt cggcgcacag gtcggccagt tcgccgggcc cgaagctgcc 9900cgtcggcagg cggtcggcca agttgccgcc gctctccgcc tcgccggcgt tgggcgccac 9960gccgcgatgg aacagcagcg gcacgtcttc cagcacggcg cggaagacat cgctgaaatt 10020cgcgttcgca ccgcaggtcg catagctgac gatatagctg gattcgcggg cgacatcggt 10080cagcacgtgg agcagcgagg tcttgccgat gccgcgctcg ccatagagca cgacatggct 10140gcgctggctc tcgatcgagg agattaggcg cgccagcacg ccgaggcgcc cggcgaagct 10200cgaccgatcg gccaccggct gggtgggtgt gaagaaggtc gccagcgcga accgggcgcg 10260cgtgatctcg cggcgctcgt cgcggcggcg atccagcggg cggtccagcg cggaggcacg 10320gaaggttggg aaatccgggc gaccacggcc gctatgggca tcgcgatgcg gcaccactgt 10380cgcagtcagc gggaaatagc cctcttcttc aggttcttct cgacggccga acggccacaa 10440gaatctcagc gcggaaccta cagccactcg aacacctctt aaattcgtgc gccatcggca 10500ccgacggcgc accctggttc gcgccccctg gcgccccctc ctaacgaacc cacgccttgc 10560ctggcctatc ggcgcttgaa gaactcgtac ggtttgatca ccaaggcgat gtacgccagg 10620accagagcga tcgtcaaaat tgcaaagacg tgataattct cattgcccag ataattggcg 10680acggcgcaac cgactgcggg cggcaaatag ctgatcatcg tgtcccggac tgccgaatcg 10740gcttgggacc gttgcaggaa tataacgatc aggccggcaa atatcgcgat ggtgacccaa 10800tcatagggcg tctgcatgca tgtcctttct attcgacacc ggaatcgaac catttccggc 10860gacgctattg cacgcactag cagtgcgcgc ggccgctcgc taggtagcgc cgcaccggat 10920aaaccgacgt taagatggcg cggctcgatc gaaatggagt caaacgggct tgcccggccg 10980accgaagcat ggcgccatgg cgcatgcacc gtattgtgac cacgcaaacc gcgagggtca 11040ttcgatgcgg ttgcttgtac aggaggccat tgataatgaa gccgagaccc gggggaacct 11100ttatgcaagt aaatttcaat cgacaggctc gcaagctcgg tgccggcaat gcgctcgcgc 11160ggggggggcc cgtgcttgcg ctgcttgcga ccgcggcatg gacacaacct gcgctggcgc 11220agcgacaggc atttgagtcc cgcccctccg gtagcgagcg acaggtcgat attcgcgcga 11280cggggtcgct ggaatatgac gacaacgtcg tgctgaacga ccagcggatc acggacggcg 11340cgcgtggcga tgtgatcgca tcgcccgggc tggacgtgac cctagttctg ccccgcgcca 11400ccgggcagct ctacctcacc ggcaatgtcg gatatcgctt ttacaagcga tataccaact 11460ttaaccgcga gcagatctcg ctcaccggcg gcgcagatca gcggttcgcc tcctgcgtcg 11520tgcacgggga agtcggctat cagcgccacc tcaccgacct gtccagcatc ttgatccagg 11580acaccacgcc tgcgctcaac aacaccgaag aggcccggca gtacaccgcg gatatcggct 11640gcggcgcgac ctacggcctg cggcctgccg tttcctacac ccgcaacgaa gtgcgcaaca 11700gccttgccga gcgccgatac gcggactcga ataccaacac ctttaccgca cagcttggcc 11760tgacttcgcc tgccctgggg accgtggcgg tatttgggcg tatgtccgac agcagctatg 11820tccatcgcgt ccttcccggc attaccggcc aggacgggat gaagagctac gcggccggcg 11880tccagctcga gcgctcggtg gccaaccgac tccatttcaa cggctcggtg aattacaccg 11940aggttgaccc aaagctcgca tccaccaaag gattcaaggg cgtaggattt aacgtttccg 12000gcgattatgc tggtgatcag tacagcctcc aattgctggc ttcacgatcg ccccagcctt 12060cacttcttct gttcgtgggt tacgagattg tgacagcggt ttcggcgaat gcgacgcgcc 12120ggctgagcga tcgcattcag atatcgctgc aaggcagccg aacctggcgc gagctcgcgt 12180cttcgcggct gctcaccaac gtgccgattt ccggcaacga caacacctcg acgttgttcg 12240cctccgctac cttccggccg aatcgccggc tgagctttgt gctgggtgcc ggccttcagc 12300ggcgcaccag caacacgcag ctatacagtt acagctccaa acgcatcaat ctctcgacgt 12360cgctttcgct ctgacaaggg ccgtaatcat gcatatcaag aatcgcttcg tgaatatctc 12420gacgttggcc atcgccgccg cgctggccac gccggcggcg gcgcagatcc ccacgcggtc 12480cgtgcccgcg ccggcccgcc cgcggcctgc aacgccgccg gcgcaacagc agaaccaggc 12540gccgtcgacg cccgcagcgg caaccccggc gcagaccgcc gcaaccgttg cccctgcagc 12600aaccgcaccc gcaggttaca aaatcggcgt ggacgacgtg atcgaggccg acgtgctcgg 12660ccagaccgac ttcaagacgc gcgcccgtgt gcaggcggac ggcacggtga ccctgcccta 12720tctgggcgcc gtgcaggtca agggcgagac cgcgacctcg ctcgccgaaa agctggccgg 12780gctgctgcgc gccggcggct attatgccaa gccgatcgtc agcgtcgaaa tcgtcggttt 12840cgtcagcaac tatgtgacgg tgctgggcca ggtgaacagt tccggcctgc agccggtcga 12900ccgcggctat cacgtttccg agatcatcgc ccgtgccggc ggcctgcgcc ccgaagcggc 12960cgatttcgtc gttctcaccc gcgccgatgg ctccagcgcc aagctggact acaagaagct 13020cgcccaaggt ggccccaatg acgatccgat ggtgacgccc ggggacaagg tctttgtccc 13080ggaagtcgag catttctaca tttatggtca aattaacgcg cctggcgtat acgcgattcg 13140atcggacatg acgctccgtc gcgcgctggc ccagggcggt gggcttgccc ccgcaggctc 13200cgtcaagcgt gtgaaggtca cgcgggatgg caatgaactc aagttgaagc tggacgatcc 13260gattctccca ggcgacacga tcgtcatcgg cgaacgattg ttctgatctt ggcaacgatg 13320gcagcggacg aggcccacca gtgaatatca ttcagttctt ccgcattctg tgggtgcgcc 13380gatggatcat cctcccggcg tttctcgttt gcgttaccac tgccaccatt gtggtccagt 13440ttctgcccga acgctacaag gccactacgc gggtggtgct cgacacgttt aagcccgatc 13500ccgtcaccgg acaggtgatg agctcgcagt tcatgcgcgc ctatgtcgag actcagaccc 13560agctgatcga ggactatgcg accgccggtc gcgtggtcga cgaactgggc tgggtgaatg 13620atccggcgaa catctccgcg ttcaacaact cgtccgcggc tgccaccggc gacatccgcc 13680gctggctcgc caagcagatc atcgacaata ccaaggccga tgtgatggag gggagcaaca 13740tcctcgaaat cacctattcg gacagctcgc ccgagcgcgc cgaacgcatc gccaacctga 13800tccgcacctc gttcctcgcc cagtcgctcg ccgccaagcg ccaggccgcg accaagtcgg 13860ccgactggta cgcccagcag gccgaagctg cccgcgattc gctcgctgcg gcggtccagg 13920cccgcaccga tttcgtgaag aagaccggca tcgtgctgac cgaaaccggc gccgacctgg 13980aaacccagaa gctccagcag atcgaggggc agacgacgac cgccaccgcc ccggttgcca 14040tggcccccag cggcatgggc ccggcgcaga tgcagctcgc ccagatcgac cagcagatcc 14100agcaggcagc gaccagccta ggtccgaacc acccaacttt ccaggccttg cagcggcagc 14160gcgaagtgtt cgccaaggca gcggcggcgg aacgcgcgca ggcgaacggc gtatccggtc 14220cggcacgcgg ggccatcgaa agcgcagcca acgcccagcg cgcgcgggtt ctcggcaatc 14280gtcaggatgt cgacaagctt acgcagctgc agcgtgacgt ctcgctgaag caggatcagt 14340acatgaaggc ggcacagcgc gtcgccgatc tgcggctgga agcaagcagc aacgatgtcg 14400gcatgtcgac gctcagcgaa gcatcggcgc cggaaacgcc ctattacccc aaggtgccgc 14460tcatcatcgg tggtgcagcc ggcttcggcc tcgggctcgg tctgctggtc gcgctgctcg 14520tcgagctgct cggccgccgc gtccgcagcc ccgaggatct ggaagttgcg atcgatgcac 14580cggtgctggg cgtgatccag agccgcgcct cgcttgccgc ccgccttcgc cgcgcccaag 14640aaaccctcgg cgaaggtgcc gacacgcacg gagcttcagt aaactgatgg acgcgatgac 14700cagcgaaccg ctgcccgaag gcgatcgtcc gagcgccgtg ccgaccacgc cggatacgat 14760cggcatgctc gaataccagc tcgtcctctc cgatccgacc gggatcgagg cggaagcgat 14820ccgcgcgcta cgcacgcgca tcatgaccca gcacctccgc gagggccggc gcgcgctcgc 14880gatctgcgcc gcctcggcgg gatccggctg cagcttcacc gccgtcaatc tggcgacggc 14940gctggcgcag atcggcgtta agactgcgct ggtcgatgcc aatctgcgcg atcccagcat 15000cggcgcagcc ttcggcctcg ccgccgacaa

gcccggcctg gccgattatc tcgcctcggg 15060cgatgtcgac ctcgcctcga tcatccatgc gacccgcctc gaccagctct cgatcatccc 15120ggccgggcat gtcgagcaca gcccgcagga actgctcgcg tccgaacagt tccatgatct 15180ggcgacgcag ctgctgcgcg agttcgacat cacgatcttc gacaccacgg cgtccaacac 15240ctgcgccgac gcgcagcgtg tcgcgcatat cgccggctat gcgatcatcg tggcgcgcaa 15300ggatgcgagc tacatccgcg acgtgaacac gctcagccgc acgctgcgtg cagaccgcac 15360caacgtcatc ggctgcgtac tgaacggcta ttgatttgga ccatatggca gcgaccgcga 15420tgacgcggca gcaggagagg aagggcggtg gctattggct ggccgttgcc ggtcttgccg 15480cgctaaccat cccgaccttc atcaccctgg gtcgcgaggt ttggagtgcg gaaggcggcg 15540tgcagggtcc gatcgtgctc gccacgggcg cctggatgct ggcccgccag tgctcgacga 15600tcgaggcgct acgccgcccc ggcagcgtgc tgctcggcgc gctgttcctg ctggcgacgc 15660ttgccttcta caccgttgga cgggtgttcg acttcatcag tgtcgaaacc ttcggactgg 15720tcgcgaccta tctggtcgtc gcctatctct atttcggtgc cagggtgctc cgtgccgcct 15780ggttcccggt gctgtggctg ttcttcctgg tgccgccgcc cggctgggcc gtcgaccgca 15840tcaccgcacc gctcaaggag ttcgtctcct atgcggcaac gggcctgctt tcctgggtgg 15900attatccgat cctgcgccag ggcgtgacac tgttcgtcgg cccctatcag ctgctcgtcg 15960aagatgcctg ttcgggtctg cgctcgctgt ccagcctggt cgtcgtgacg ctgctctaca 16020tctacatcaa gaacaagccg tcctggcgct acgcggcgtt catcgcagcg ctggtgatcc 16080cggtggcagt ggtgaccaac gtcctgcgga tcatcatcct ggtactgatc acctatcatc 16140tgggcgacga ggcggcgcag agcttcctcc acgtctccac cggcatggtg atgttcgtgg 16200tcgccctgct ttgcatcttc gcgatcgact gggtggtcga gcaacttctt ctcctgcgtc 16260ggaggcatca tgttcaaccg gcgtgacctg ctgatcggcg caggctgctt cgccgccgct 16320ggcgcctcgc tcggcctgaa gccgcaccgg cggatggacc tgctgggcgg caccaagctc 16380gacacgctga tgcccaaggc attcggcgca tggaaggcag aggataccgg ttcgctgatc 16440gcgccggcgc gcgaaggcag cctggaggac aagctctaca accaggtggt cacccgcgcc 16500ttctcccgcg cggacggtgc ccaagtgatg ctgctgatcg cctatggcaa cgcccagacc 16560gatctactgc agctgcaccg gccggaaata tgctacccgt tcttcggctt caccgtggtg 16620gaaagccatg agcagaccat cccggtgacg ccgcaggtga cgatccccgg tcgcgcgctg 16680accgccacca acttcaaccg caccgagcag atcctctact ggacccgcgt cggcgaatat 16740ctgccgcaga acggcaatca gcagatgctc gcgcggctga agagccaggt ccagggctgg 16800atcgtcgacg gtgtgctggt gcgcatctcg acggtgacgc ccgaggcgga agatggcctg 16860agcgccaatc tcgatttcgc gcgcgagctg gtgaagacgc tcgacccgcg cgtgctgcgc 16920ccgctgctcg ggaacgggct cacacggcag ctcggtcacc aggtctgaac cggtgcgccg 16980cacgcggcgc ccccggcaac aaaaaaggag cggcgcgggc cgccgccgct ccctctcctt 17040ctcatgcggc gccctgccct caccgctcgt gcagcgcgtc actccccgtc tcgagcacgg 17100gccccaccag atagctgaac agggttcgct tgccggtgac gatgtccgcg ctcgcgagca 17160tccccggccg cagcggcacc tgtgcgccat gggccagcac atacccgcgc gccagcgcga 17220tccgcgcctt gtagaccggc ggctggttct ccttcatctg caccgcctcg gggctgatgc 17280ccgccaccgt gccgggaatc atgccgtagc gggtataggg aaaggcctgc agcttcacct 17340ttaccggcat gccgatgtgg acgaagccga tgtcgctgtt gtcgaccatc acctcggcct 17400cgagccgggc attgtcggga accaggctga ggagcggctt ggccccttcc accacgccgc 17460cttcggtgtg gacctgcagc tgcgagacgg taccgctcac cggcgcgcgc agttcgcgga 17520acgagctgcg cagattcgcc ttggcgacgt cctcgccgcg ggcacgcacc tcgtcctgcg 17580ccttgaccag atcctgcagc acctgcgccc gcgcctcctc gcgcgtcttg gccgacaggc 17640tggagacgct cagcgactgc tggccgagtt tggcgagcgt agcgcgcgcc gccgtcaggt 17700cctgccgctc ggcgatcagc tggcgacgca tctccacgac gcgcagcttc gagacatagc 17760ccttggcggc catcgtctcg ttcgcggcga tctgctgttc gagcagcggc agcgactgtt 17820cgagcttccg cacctgtgcc tgcgcctcgg ccgcggccga gacggcggca ccgcgatcgg 17880agcggccgcc ggccagcgcc gcctcgatct ggcccagccg ggcgcgggcg aggccgcgat 17940gcgtcgccac ttcgcccggg ctggcggcgg caggcgcgac gaagcggaag cccctgccgt 18000ccagcgcgtc gatgatcgcc tggttgcgtg cggcgtcgag ctgggcgctg agcagcgcca 18060ccttcgcctg tgccgcctcc gccgacgaca cggtcgggtc gagcgtgatc agcacctggc 18120ccttggcgac cttctgcccc tcgcccacca ggatgcggcg gacgatcccc gattcgggcg 18180actggacgat cttggtctcg ccgatcggcg cgatccgccc ctgcgtcggc gcgacgactt 18240cgaccttgcc gatcgccagc caggcggcgg tgatcgccag cccggccagc atcaccttgg 18300cggtaagccg cgcggtgggc gaaaccggcc gctcgatgat ctccagcgcg gcaggcagga 18360aggcggtgtc ataagcgtcg acgcgggcag gcagcacggt atcgcgcatg cgggcgagcg 18420ggccgccgcg gcgcatcgga acaacggcgt tcatgcggca atctccccat agccgccctg 18480gcggcggtgc aggtcggcat agcggccgcc caggcgcaac aattcgtcgt gtcggccgct 18540ctcgacgatg cggccctgtt cgagcgtgat gatccggtcg cagctgcgca ccgcgctcag 18600gcgatgcgcg atcaccacga gcgtgcggcc ggccgagatg gcgcgcaggt tgttctggat 18660cagctcctcg ctctcggcat cgagcgccga ggtcgcttcg tcgaacacca ggatgcgcgg 18720attgccgacg agcgcgcggg cgatggcgag ccgctggcgc tggccgccgg agagattgac 18780gccgcgctcg acgatctcgg tgtcatagcc gcgcggctgg cgcaggatga aatcatgcgc 18840gccggccagc gtcgccgccg cgacgacatt ctcgaacggc atggcggggt tggagagcgc 18900gatgttctcg cggatcgagc ggctgaacag cagattctcc tgcagcacga cgccgatctg 18960gcgacgcagc caggcgggat cgagctgcgc cacgtcgacc tcgtcgacca gcacgcggcc 19020gagattcggc aggttgagcc gctggagcag cttggccagc gtcgacttgc ccgagcccga 19080cgaaccgacg atgccgagcg aggtgcccgc cggaatgtcg agcgtgatgt cgctcagcac 19140cggcggctgg tcctcggcat agcggaagct gacattctcg aagcgaatcg caccgcgcag 19200caccggcagc gtcgccgccg aggccgggcg cggttccacc ggatggttga gcacgtcgcc 19260cagccgctcg accgagatgc gcacctgctg gaaatcctgc cacagctgcg ccatgcggat 19320caccggcccg gacacgcgct gggcgaacat gttgaacgcc accagcgcgc ctacgctcat 19380cgcgccgccg atcaccgcct tggcgccgaa gaacaggatc gccgcgaagc tcagcttcga 19440gatcagctcg atcgcctggc tgccggtgtt ggcggtattg atcagccgct gcgacgcggc 19500ggtatgggcg gcgagctggc gctcccagcg attctgccag tgcggctcga ccgcggtcgc 19560cttgatcgtg tggatgcccg agacgctctc gacgagcagc gcgttgctgg cggagctctt 19620ctcgaacttg tcctccaccc gcgcgcggag cggcccggcg acgctgaacg atacgatcgc 19680ataggcgatc agcgacacga gcacgatgcc cgagagcatc ggcgagtaga acagcatcgc 19740ggcgaggaac acgaaggtga acagcgggtc caccatcacc gtcagcgagg cgctggtaag 19800gaattcgcgg atcgtctcga gctggcggac gcgggtgacg gtgtcgccca cgcggcgctt 19860ctcgaaatag gcgagcggca gcgccagcag gtggtggaac agccgggcac ccagctcgac 19920gtcgatcttc tgcgtcgtct cggtgaacag gcgggtgcgg atccagccga gcgccacttc 19980ccacaccgaa accgccagga aggcgaaggc gagcacgctc agcgtgctca tgctgttgtg 20040gatcagcacc ttgtcgatca cgctctggaa caacagcggc gcggcgaggc cgagcaggtt 20100gagcgcgagg gtgatgccga gcacctcgag gaacagcgtg cgatagcgcc ggaactgcgc 20160ggtgaaccag gagaggccga accgcagcgg ccgtcccgcc accgcgcggg tggtgagcag 20220caccagcgcg ccggaccaga tcgcgtccag cgcgtcccgg tcgacctgtt ccggggcatg 20280gcccgggcgc tggatgatca cgccatgttc ggtcaggccg ccgatcacga accagccttc 20340gggcccgtcg gcgatcgcgg gcagcggctg gcgggcgagt ccgccgcgcg gcacctcgac 20400ggccttggcg cgcacgccct gctggcgctt ggccaggagg atcaggtcgt cggcgcttgc 20460cgcctcggca tggcccagcg cgtggcgcag ctgttcgggc gtgatggcga tgttgtgcgc 20520gccgagcagc agcgacaacg ccaccagtcc ggattcgcgc agctccgcct cgcgctccgc 20580cgccccatgg gccgcgagcg cgctctgcag ggtggcctgc atttcgtcgc gtgtcatttc 20640cggaactctg cctccatggc gatactgaga gcgccatgat gaagaaggct ggtaaagact 20700cacttaatcc tagcttttct ggtatttacc cgtagctgcc gacccgattt gggacaggcc 20760tggcttagca ggtccttaaa ctcgaccgac tataccgcga cgccgaggag ggggaggatt 20820ggcgccgcat cgcgcggcga aacgcgggtg cgtcgcaaca tttcgccgga gtcgatccgt 20880cgcgaatgct gcacccgcga acgcaatgac ggccgccacg caatccggct tgatcccggg 20940cggcggatcg cgataagccg cgccacggtc gccaaaactc gtcgaaataa ccgacaaaac 21000cacggcatat ggctggatat tgcagcgttt gccctgcgtt tccgtcgttc aaccgccctt 21060cgaatcaggc aggcccagcg tgaccatgat tgatcttcct cttggaacgg cacactttgg 21120tcgacacgga gacttccggt cgggcaattg tcccgttata gtgcaatgca acaggccgaa 21180tcggccgctg tcggcgtgca cattccgttg agggagcccg atgaggcaat gaacgctttc 21240gaagcacagc gcgcctttga ggagcaactt cgggcgcatt cccgggttac gccatctgcc 21300gctcccgtgt ggcgtcgctc gacgctgcgg atggtcctct ataccgagtt gctgctgctg 21360gacagtctct cgatcctggc cggattccac gtcgcggcgg gcacgcgcga cggcaactgg 21420ctgtcgctgg cgggcatcaa cgtcggcgtc ttcctgctgc cgatcgctct cggcaccgcg 21480ctcgcaagcg gcacctactc gctgaactgc ctgcgctacc cggtcagcgg cgtgaagagc 21540atcttctcgg cattcttctt ctcgatcttc gtcgtcctgc tcggcagcta cctgctgacg 21600gccgagctgc cgctgtcccg cgtgcagctg gcggagggcg cgatcctctc gctggtcctc 21660ctgatggtgg gccgcctgat gttccgccgc cacgtccgcg cggttaccgg cggcaggctg 21720ctcgacgaac tggtcatcat cgacggcgtc tcgctcgacg tcgcgggcaa tgcggtcgcg 21780ctcgacgcgc ggatcatcaa tctctcgccg aacccgcgcg atccgcaaat gctgcatcgc 21840ctgggcacca ccgtgatcgg gttcgaccgg gtgatcgtcg cctgcaccaa ggagcatcgc 21900gcggtctggg cgctgctgct caagggcatg aacatcaagg gcgagatcct cgtcccccag 21960ttcaatgcgc tgggcgcgat cggcgtggac gcctttgacg ggaaggatac gctggtcgtc 22020tcgcagggcc cgctcaacat gcccaaccgc gcgaagaagc gcgcgctcga tctcgcgatc 22080accgtaccgg ccgtgctcgc gctggcgccg ctgatgatcc tggtggcgat cctgatcaag 22140ctggagagcc cgggcccggt gttgttcgcg caggatcgcg tcggccgcgg caaccggctg 22200ttcaagatca tgaagttccg ctcgatgcgc gtaacgctgt gcgacgcgaa cggcaacgtc 22260tcggccagcc gcgacgacga tcgcatcacc aaggtcggcc gcttcatccg caagaccagc 22320atcgacgaac tgccgcagct gctgaacgtg ctgcgcggcg acatgagcgt cgtcggcccg 22380cggccgcatg cgctgggctc gcgcgccgcc gatcacctgt tctgggaaat cgacgagcgc 22440tactggcacc gccacacgct caagccgggc atgaccggtc tggcccaggt gcgcggtttc 22500cgcggggcga ccgatcgccg cgtcgatctg accaaccggc tccaggcaga catggaatat 22560atcgacggat gggatatctg gcgcgatatc acgatcctgt tcaagacgct gcgggtgatc 22620gtgcattcga acgcattctg atccgcgcac gacgctgggc cgcagcctcg atccgcaaat 22680ggattgacag cggcccggct tccgttttct cgtttgattt tcgttgcggc cggtccgcgc 22740catgggggat tactgaatga agggcatcat ccttgcgggg ggcagcggga cgcgcctgta 22800ccccgcaacg ctatcgatct cgaagcagct gcttcccgtc tatgacaagc cgatgatctt 22860ctatccgctg tcggtgctga tgctcaccgg catccgggac atcctgatta tctccacccc 22920gcgcgacctg ccgatgttcc aggcgctgct gggcgacggc tcggccttcg gcatcaacct 22980cagctatgcc gagcagccct cccccaacgg gctggccgaa gcgttcatca tcggcgcgga 23040tttcgtcggc aacgatccca gcgcgctgat cctgggcgac aacatctatc acggcgaaaa 23100gatgggcgag cgctgccagg cagccgcagc gcaggcagcg cagggcggtg caaacgtctt 23160cgcctatcat gtcgacgacc ccgagcgcta cggcgtggtc gcgttcgacc cggagacggg 23220cgtcgccacc agcgtcgagg aaaagccggc cgagcccaag tccaactggg cgatcaccgg 23280cctgtatttc tacgacaagg acgtggtcga catcgccaag tcgatccagc cctcggcgcg 23340cggcgaactc gagatcaccg acgtcaaccg cgtttacatg gagcgcggcg acctgcacat 23400cacgcgcctc ggccgcggct atgcctggct cgacaccggc acgcatgaca gcctgcacga 23460agccggctcg ttcgttcgca cgctcgagca tcggacgggc gtgaagatcg cctgcccgga 23520ggaaatcgcc ttcgaaagcg gctggctcgg cgccgaagac ctgctcaagc gcgccgccgg 23580cctcggcaag accggctatg ccgcctatct ccgcaaggtt gcgaccgcag catgacccag 23640gtccatcatc acgaactgtc cggcgtcatc gagttcacgc cgcccaaata tggcgaccac 23700cgcggcttct tctccgaagt gttcaagcag tcggtgctcg atgccgaagg cgtcgaggca 23760cgctgggtgc aggacaatca gagcttctcg gcggccccgg gcacgatccg cggcctgcat 23820ctccaggcgc cgcccttcgc ccaggccaag ctggtccgcg tgttgcgcgg cgcgatcttc 23880gacgtcgcgg tcgacatccg tcgcggctcg cccacctatg gcaaatgggt cggcgtcgag 23940ctctcggccg agaagtggaa ccagctgctg gtccccgccg gctatgcgca cggcttcatg 24000acgctcgttc cggattgcga gatcctctac aaggtcagcg ccaaatattc gaaggattcg 24060gagatggcga tccgttggga cgatcccgat ctcgccatcg cctggccgga catcggcgtc 24120gagccggtcc tctccgaaaa ggacgcggtc gccacgccct tcgccgaatt caacaccccc 24180ttcttctatc agggctgagc catgcagcag accttcctcg tcaccggcgg cgccggcttc 24240atcggctcgg cggtggtgcg ccacctcgtc cgccagggcg cgcgcgtcat caatctcgac 24300aagctcacct atgccggcaa cccggcctcg ctgactgcga tcgagaacgc gcccaactat 24360cgcttcgtcc atgccgacat cgccgacacc gcgacgatcc taccgctgct gcgcgaggag 24420caggtcgatg tggtgatgca cctcgccgcc gagagccatg tcgatcgctc gatcgacggc 24480cctggcgagt tcatcgagac caatgtcgtc ggcaccttca agctgctcca gtcggcgctg 24540caatattggc gcgagctgga gggcgagaaa cgcgacgcgt tccgcttcca ccacatctcc 24600accgacgaag tgttcggcga cctgccgttc gacagcggca tcttcaccga agagacgccc 24660tatgatccct cctcgcccta ttcggcgtcg aaggcggcga gcgaccatct ggtgcgcgcc 24720tggggccaca cctatggcct gccggtggtg ctgtcgaact gctcgaacaa ttacgggccg 24780ttccacttcc ccgagaagct gatcccgttg accatcctca acgcgctcga gggcaagccg 24840ctgccggtct acggcaaggg cgagaatatc cgcgactggc tgtatgtcga cgatcacgcc 24900aaggcgctgg cgaccatcgc caccaccggc aaggtcggcc agagctacaa tgtcggcggc 24960cgcaacgagc ggaccaacct gcaggtggtc gagacgatct gcgacctgct cgaccagcgc 25020attccgctgg ccgacggtcg caagcgccgc gaactgatca ccttcgtcac cgatcgcccc 25080ggccatgacc gccgctacgc gatcgacgcg accaagctcg agaccgagct gggctggaag 25140gctgaggaga atttcgacac cggcatcgcc gcgacgatcg actggtatct ggcgaacgag 25200tggtggtggg gcccgatccg ctccggcaaa tatgccggcg agcggctggg gcagaccgcc 25260tgatgcgtat cctcgtcacc gggcatgacg gccaggtcgc ccagtcgctg gccgagcagg 25320cggtgggcca cgagctggtc ttcaccacct accccgaatt cgatctctcc aagccggaga 25380cgatcgaggc cggtgtggcg cgggtgcacc cggacctgat cgtctccgcc gccgcctaca 25440cggcggtcga caaggcggaa agcgaacccg agctggcgat ggcgatcaac ggcgacggtc 25500ccggcgtgct ggcgcgcgcg ggcgcgaaga tcggcgcgcc gatcatccac ctgtcgaccg 25560attatgtgtt cgacggcagt ctcgaccgcc cttggcgcga ggacgatccc accggcccgc 25620tcggcgtcta tggcgcgacc aagctggccg gcgagcaggc ggtgcaggcc tcgggtgcca 25680ccaacgccgt gatccggctg gcctgggtct acagcccgtt cggcaacaat ttcgtcaaga 25740cgatgctccg cctcgccgag acgcgcgacg cgctgaacgt cgtggaggac cagtggggct 25800gccccagttc ggcgctggac atcgcgaccg cgatcctgac ggtggtcggg cactggcagc 25860aggacggcgc gacgagcggc ctctaccatt tcgccggcac cggcgagacc aactgggccg 25920acttcgcatc gacgatcttc gccgagagcg ccaagcgcgg tggcccctcg gccaccgtca 25980ccggcattcc cagctcgggc tatccgactc cggccacgcg cccggccaat tcgcggctgg 26040actgcacccg cttcgcggag accttcggct accgggcgcc tgcctggcag gattcgctga 26100acgtcgtact ggatcgcctg ctcggctgat ccgaaacggg gggcctcagc gccccccgcc 26160atgctcccgt tcgcgcgccg gcaatgcctc tagcaccgcg cgctttccct taggactcag 26220ctcgctccag ccggcgattt ccttgggcga ccgccagcac cccaggcaca gccggatc 2627821053DNASphingomonas sp. ATCC53159CDS(1)..(1053) 2atc aac ggc gcc ttg ctc gga cgg cac aaa ttc gtc ctg gtc aat gtg 48Ile Asn Gly Ala Leu Leu Gly Arg His Lys Phe Val Leu Val Asn Val 1 5 10 15 tcc acg gtc gcc tct tcg ata ctg ttc cag ctg ttc ccg ctt gtc gtc 96Ser Thr Val Ala Ser Ser Ile Leu Phe Gln Leu Phe Pro Leu Val Val 20 25 30 gcc tgg atg atc ggc ccg gac ctg cga acg ctg ctg atc gcc gcg ctc 144Ala Trp Met Ile Gly Pro Asp Leu Arg Thr Leu Leu Ile Ala Ala Leu 35 40 45 gtt ggc cgg gcg gtt ccg atg atc ggc atg ctg ccc gcg ctg tat cga 192Val Gly Arg Ala Val Pro Met Ile Gly Met Leu Pro Ala Leu Tyr Arg 50 55 60 aac ctt ttg cgc ggc aac acg ccg cgt ttt cac gcc agc gag gcg cgc 240Asn Leu Leu Arg Gly Asn Thr Pro Arg Phe His Ala Ser Glu Ala Arg 65 70 75 80 ttc ctg ata ggc tat ggc ggg tgg gcc tcg ctc acg acc gtg gta gcg 288Phe Leu Ile Gly Tyr Gly Gly Trp Ala Ser Leu Thr Thr Val Val Ala 85 90 95 acc gtg ctc atg atg gcg gac cgc ttc ctg att ggc gca ctt ctt ggg 336Thr Val Leu Met Met Ala Asp Arg Phe Leu Ile Gly Ala Leu Leu Gly 100 105 110 ccc gtc gcc gtg acc atc tac acg gcc ccc ctg caa ctc gca cag cgc 384Pro Val Ala Val Thr Ile Tyr Thr Ala Pro Leu Gln Leu Ala Gln Arg 115 120 125 gta tcg ctg ctg ccc tcc gca ctg tcc gcc gcg ctg ttc ccg cgc ctg 432Val Ser Leu Leu Pro Ser Ala Leu Ser Ala Ala Leu Phe Pro Arg Leu 130 135 140 ccc agc gcg acg ccg gcg gag cgc atg gcg ctt cag atc cgc tcg ctg 480Pro Ser Ala Thr Pro Ala Glu Arg Met Ala Leu Gln Ile Arg Ser Leu 145 150 155 160 tcg ctg atc atg ggc ggc ctt acc ggg atg atc ggc ggc gga cta ttg 528Ser Leu Ile Met Gly Gly Leu Thr Gly Met Ile Gly Gly Gly Leu Leu 165 170 175 ctg gcc gcg ccg ttt ctc gat ctc tgg atc ggc aag tcg ctc ggc cat 576Leu Ala Ala Pro Phe Leu Asp Leu Trp Ile Gly Lys Ser Leu Gly His 180 185 190 gcg gga acg ccg gtc gcg ctc ttc ctg ttc ttc ggc gca tgg tgg aat 624Ala Gly Thr Pro Val Ala Leu Phe Leu Phe Phe Gly Ala Trp Trp Asn 195 200 205 gcg ctg gcg atc att tcg ttc agc ggc ctg cag gcg agc gga cgg ccg 672Ala Leu Ala Ile Ile Ser Phe Ser Gly Leu Gln Ala Ser Gly Arg Pro 210 215 220 aaa gcg agc gcg atc gtc cag ggg gca gag ctg cta ccc gtg ttg atc 720Lys Ala Ser Ala Ile Val Gln Gly Ala Glu Leu Leu Pro Val Leu Ile 225 230 235 240 gcg ctg tat gca ggg atc cga tgg ggc ggc gtg acc ggc gcc gca gcg 768Ala Leu Tyr Ala Gly Ile Arg Trp Gly Gly Val Thr Gly Ala Ala Ala 245 250 255 gtc ttt ctg gga cgc tcc gcc ctg gat ttc gtc ctg ttg acc tgg cag 816Val Phe Leu Gly Arg Ser Ala Leu Asp Phe Val Leu Leu Thr Trp Gln 260 265 270 gca ggc ctg ctc cgc cag acg gtg aag caa gta tcc gta tgc ggc gcc 864Ala Gly Leu Leu Arg Gln Thr Val Lys Gln Val Ser Val Cys Gly Ala 275 280 285 gtt ctc acc gtc gcg atg ctc gtg ggc gcg acc tat cgc tat tcg gtg 912Val Leu Thr Val Ala Met Leu Val Gly Ala Thr Tyr Arg Tyr Ser Val 290 295 300 ccg ctc tgg tgc gta ctc agc gcc tgc tgc ctg gtc gcg ctg gca gcc 960Pro Leu Trp Cys Val Leu Ser Ala Cys Cys Leu Val Ala Leu Ala Ala 305 310 315 320 tgc tcc tgg tgg aca ttg gcg cgc cag gac aag gca ctg ctg att gga 1008Cys Ser Trp Trp Thr Leu Ala Arg Gln Asp Lys Ala Leu Leu Ile Gly 325 330 335

cga ttg agc cga att cta cca aag cag cgg caa ctc gac cta tag 1053Arg Leu Ser Arg Ile Leu Pro Lys Gln Arg Gln Leu Asp Leu 340 345 350 3350PRTSphingomonas sp. ATCC53159 3Ile Asn Gly Ala Leu Leu Gly Arg His Lys Phe Val Leu Val Asn Val 1 5 10 15 Ser Thr Val Ala Ser Ser Ile Leu Phe Gln Leu Phe Pro Leu Val Val 20 25 30 Ala Trp Met Ile Gly Pro Asp Leu Arg Thr Leu Leu Ile Ala Ala Leu 35 40 45 Val Gly Arg Ala Val Pro Met Ile Gly Met Leu Pro Ala Leu Tyr Arg 50 55 60 Asn Leu Leu Arg Gly Asn Thr Pro Arg Phe His Ala Ser Glu Ala Arg 65 70 75 80 Phe Leu Ile Gly Tyr Gly Gly Trp Ala Ser Leu Thr Thr Val Val Ala 85 90 95 Thr Val Leu Met Met Ala Asp Arg Phe Leu Ile Gly Ala Leu Leu Gly 100 105 110 Pro Val Ala Val Thr Ile Tyr Thr Ala Pro Leu Gln Leu Ala Gln Arg 115 120 125 Val Ser Leu Leu Pro Ser Ala Leu Ser Ala Ala Leu Phe Pro Arg Leu 130 135 140 Pro Ser Ala Thr Pro Ala Glu Arg Met Ala Leu Gln Ile Arg Ser Leu 145 150 155 160 Ser Leu Ile Met Gly Gly Leu Thr Gly Met Ile Gly Gly Gly Leu Leu 165 170 175 Leu Ala Ala Pro Phe Leu Asp Leu Trp Ile Gly Lys Ser Leu Gly His 180 185 190 Ala Gly Thr Pro Val Ala Leu Phe Leu Phe Phe Gly Ala Trp Trp Asn 195 200 205 Ala Leu Ala Ile Ile Ser Phe Ser Gly Leu Gln Ala Ser Gly Arg Pro 210 215 220 Lys Ala Ser Ala Ile Val Gln Gly Ala Glu Leu Leu Pro Val Leu Ile 225 230 235 240 Ala Leu Tyr Ala Gly Ile Arg Trp Gly Gly Val Thr Gly Ala Ala Ala 245 250 255 Val Phe Leu Gly Arg Ser Ala Leu Asp Phe Val Leu Leu Thr Trp Gln 260 265 270 Ala Gly Leu Leu Arg Gln Thr Val Lys Gln Val Ser Val Cys Gly Ala 275 280 285 Val Leu Thr Val Ala Met Leu Val Gly Ala Thr Tyr Arg Tyr Ser Val 290 295 300 Pro Leu Trp Cys Val Leu Ser Ala Cys Cys Leu Val Ala Leu Ala Ala 305 310 315 320 Cys Ser Trp Trp Thr Leu Ala Arg Gln Asp Lys Ala Leu Leu Ile Gly 325 330 335 Arg Leu Ser Arg Ile Leu Pro Lys Gln Arg Gln Leu Asp Leu 340 345 350 41626DNASphingomonas sp. ATCC53159CDS(1)..(1626) 4atg acg act acc tcg gcg ttt cgt cgc ccg gcc ttc cac gga gcg atg 48Met Thr Thr Thr Ser Ala Phe Arg Arg Pro Ala Phe His Gly Ala Met 1 5 10 15 cag cgg ctt cgc agg ttg cga ctg gtt cgg ttt ctg aca aag cca gcg 96Gln Arg Leu Arg Arg Leu Arg Leu Val Arg Phe Leu Thr Lys Pro Ala 20 25 30 atc ccg gta ctg ccc gtc tac aaa gcc gag cga tca ggc gtg acg atc 144Ile Pro Val Leu Pro Val Tyr Lys Ala Glu Arg Ser Gly Val Thr Ile 35 40 45 gcg gcg cgg cgt acc gtt ctg ctg gtc agc gtg atg ttt ctt gcc gca 192Ala Ala Arg Arg Thr Val Leu Leu Val Ser Val Met Phe Leu Ala Ala 50 55 60 gtc tac ggc ctg ctc gcc gca gtt ctg ccg ctc cag atg ctg gcg atc 240Val Tyr Gly Leu Leu Ala Ala Val Leu Pro Leu Gln Met Leu Ala Ile 65 70 75 80 ccg gcc gtg ccc ctc gtt ctg atg gcg ctc gta gtg atc tgg gcg cta 288Pro Ala Val Pro Leu Val Leu Met Ala Leu Val Val Ile Trp Ala Leu 85 90 95 ccc gag gcg cgg cag gcg cct act cgc ctg ctg gca aaa cta tac ctc 336Pro Glu Ala Arg Gln Ala Pro Thr Arg Leu Leu Ala Lys Leu Tyr Leu 100 105 110 gcc tat atg gtg gcg gcg ctc gtg tgg ccg aac tat ctt gcg ctc agc 384Ala Tyr Met Val Ala Ala Leu Val Trp Pro Asn Tyr Leu Ala Leu Ser 115 120 125 gtt ggt ggt ttg cct tgg atc tcg atc agg cgg atg atc ggt tcg atc 432Val Gly Gly Leu Pro Trp Ile Ser Ile Arg Arg Met Ile Gly Ser Ile 130 135 140 gca ttg ctc acg ctc atg atc tcg ctt tcg gtc tcg aaa aag ttt cga 480Ala Leu Leu Thr Leu Met Ile Ser Leu Ser Val Ser Lys Lys Phe Arg 145 150 155 160 tcc gag atg gcg gcg att atg cgg gcg gcg ccg atc cca tcg cga cta 528Ser Glu Met Ala Ala Ile Met Arg Ala Ala Pro Ile Pro Ser Arg Leu 165 170 175 ttg ctt gcc ttc att atg gtc cag atc gtc gcc tcc att gcg acc cca 576Leu Leu Ala Phe Ile Met Val Gln Ile Val Ala Ser Ile Ala Thr Pro 180 185 190 gcg gcc tcg caa aca att ccg cgg ttg att ggc atc gtt ctg acg gtg 624Ala Ala Ser Gln Thr Ile Pro Arg Leu Ile Gly Ile Val Leu Thr Val 195 200 205 acg ccg atg gca ttc atc tcg cta tgg ctg atc ggg acc gac acg cgc 672Thr Pro Met Ala Phe Ile Ser Leu Trp Leu Ile Gly Thr Asp Thr Arg 210 215 220 acc ccg gag tgg tgg gtt acg cgc ttg ttt tgg tgc gtg ggc gtg ctg 720Thr Pro Glu Trp Trp Val Thr Arg Leu Phe Trp Cys Val Gly Val Leu 225 230 235 240 atg gcg att ggc gtg ctg gag ttc cgg gtg aag cac gtc ctc tgg gcc 768Met Ala Ile Gly Val Leu Glu Phe Arg Val Lys His Val Leu Trp Ala 245 250 255 tac tcg att ccg agc ttc ctg cgc gtt gat gag cag ttt ctt acc gta 816Tyr Ser Ile Pro Ser Phe Leu Arg Val Asp Glu Gln Phe Leu Thr Val 260 265 270 gtg ctt acg ccc ggt ttc cgg ggt act tac cgc gtt ttg acg act ttc 864Val Leu Thr Pro Gly Phe Arg Gly Thr Tyr Arg Val Leu Thr Thr Phe 275 280 285 agc agt ccg ctt gtc tgg gga gag ttg acg gct ttg acg att cct ttc 912Ser Ser Pro Leu Val Trp Gly Glu Leu Thr Ala Leu Thr Ile Pro Phe 290 295 300 gtg ctg cac cgt ata gcg aat tcc cgt ggc gta ggg aga ttg gca ttt 960Val Leu His Arg Ile Ala Asn Ser Arg Gly Val Gly Arg Leu Ala Phe 305 310 315 320 tgg att ttc ttc gat ttt ctt gta gtt gcg tcc gga ttt ctt agc ggt 1008Trp Ile Phe Phe Asp Phe Leu Val Val Ala Ser Gly Phe Leu Ser Gly 325 330 335 tct cga ctg gca atg gtg ggg gga ttg gtg gcg cat acc gtg tat ttg 1056Ser Arg Leu Ala Met Val Gly Gly Leu Val Ala His Thr Val Tyr Leu 340 345 350 ctt att tgg gca att cgg cgg tgg cgg acc aca aag ggc ggg ctt gtc 1104Leu Ile Trp Ala Ile Arg Arg Trp Arg Thr Thr Lys Gly Gly Leu Val 355 360 365 ggc att tcg ctg acg ttg acg tat ccc gcc ttg atg gtg gcg ctg tcg 1152Gly Ile Ser Leu Thr Leu Thr Tyr Pro Ala Leu Met Val Ala Leu Ser 370 375 380 ctg gcg gtc atg ttc gtg cct gcc gtc cac aac cgt gtt ctc ggt gga 1200Leu Ala Val Met Phe Val Pro Ala Val His Asn Arg Val Leu Gly Gly 385 390 395 400 ggc gct tct caa ctt agc aat caa ggc agg cag gag cag ttt cgc ttg 1248Gly Ala Ser Gln Leu Ser Asn Gln Gly Arg Gln Glu Gln Phe Arg Leu 405 410 415 ggc gtg cct gcc atc gct cgc cgc cct ttc ttc ggc tat ggc ccg gga 1296Gly Val Pro Ala Ile Ala Arg Arg Pro Phe Phe Gly Tyr Gly Pro Gly 420 425 430 gag ggt gct ggc gct gtc ggg tgg cgt aat cag caa ggc ttc cta tcg 1344Glu Gly Ala Gly Ala Val Gly Trp Arg Asn Gln Gln Gly Phe Leu Ser 435 440 445 atc gac agt ggt ttc ctg tcc gta gcg gct gat tac ggc ttg ctg ggt 1392Ile Asp Ser Gly Phe Leu Ser Val Ala Ala Asp Tyr Gly Leu Leu Gly 450 455 460 ttt gtt tcg cta tac ggc acg atg att acg tta atg att ttg ctc gcg 1440Phe Val Ser Leu Tyr Gly Thr Met Ile Thr Leu Met Ile Leu Leu Ala 465 470 475 480 ttt cgt ggt ctt aag atg agc gga gat ggc tac ccg ctt gag ctc gcc 1488Phe Arg Gly Leu Lys Met Ser Gly Asp Gly Tyr Pro Leu Glu Leu Ala 485 490 495 gta gca act ttc ctg gct gtt ctt tta aat acg cgg tca gtc ctg tcg 1536Val Ala Thr Phe Leu Ala Val Leu Leu Asn Thr Arg Ser Val Leu Ser 500 505 510 caa ggt gac aat gac ccg ttc atc ttt atg acg ctg ggg cta ggc ata 1584Gln Gly Asp Asn Asp Pro Phe Ile Phe Met Thr Leu Gly Leu Gly Ile 515 520 525 gcg ctt cta tat cgc tct cgg cct gtg tct ttg tcg gtg tag 1626Ala Leu Leu Tyr Arg Ser Arg Pro Val Ser Leu Ser Val 530 535 540 5541PRTSphingomonas sp. ATCC53159 5Met Thr Thr Thr Ser Ala Phe Arg Arg Pro Ala Phe His Gly Ala Met 1 5 10 15 Gln Arg Leu Arg Arg Leu Arg Leu Val Arg Phe Leu Thr Lys Pro Ala 20 25 30 Ile Pro Val Leu Pro Val Tyr Lys Ala Glu Arg Ser Gly Val Thr Ile 35 40 45 Ala Ala Arg Arg Thr Val Leu Leu Val Ser Val Met Phe Leu Ala Ala 50 55 60 Val Tyr Gly Leu Leu Ala Ala Val Leu Pro Leu Gln Met Leu Ala Ile 65 70 75 80 Pro Ala Val Pro Leu Val Leu Met Ala Leu Val Val Ile Trp Ala Leu 85 90 95 Pro Glu Ala Arg Gln Ala Pro Thr Arg Leu Leu Ala Lys Leu Tyr Leu 100 105 110 Ala Tyr Met Val Ala Ala Leu Val Trp Pro Asn Tyr Leu Ala Leu Ser 115 120 125 Val Gly Gly Leu Pro Trp Ile Ser Ile Arg Arg Met Ile Gly Ser Ile 130 135 140 Ala Leu Leu Thr Leu Met Ile Ser Leu Ser Val Ser Lys Lys Phe Arg 145 150 155 160 Ser Glu Met Ala Ala Ile Met Arg Ala Ala Pro Ile Pro Ser Arg Leu 165 170 175 Leu Leu Ala Phe Ile Met Val Gln Ile Val Ala Ser Ile Ala Thr Pro 180 185 190 Ala Ala Ser Gln Thr Ile Pro Arg Leu Ile Gly Ile Val Leu Thr Val 195 200 205 Thr Pro Met Ala Phe Ile Ser Leu Trp Leu Ile Gly Thr Asp Thr Arg 210 215 220 Thr Pro Glu Trp Trp Val Thr Arg Leu Phe Trp Cys Val Gly Val Leu 225 230 235 240 Met Ala Ile Gly Val Leu Glu Phe Arg Val Lys His Val Leu Trp Ala 245 250 255 Tyr Ser Ile Pro Ser Phe Leu Arg Val Asp Glu Gln Phe Leu Thr Val 260 265 270 Val Leu Thr Pro Gly Phe Arg Gly Thr Tyr Arg Val Leu Thr Thr Phe 275 280 285 Ser Ser Pro Leu Val Trp Gly Glu Leu Thr Ala Leu Thr Ile Pro Phe 290 295 300 Val Leu His Arg Ile Ala Asn Ser Arg Gly Val Gly Arg Leu Ala Phe 305 310 315 320 Trp Ile Phe Phe Asp Phe Leu Val Val Ala Ser Gly Phe Leu Ser Gly 325 330 335 Ser Arg Leu Ala Met Val Gly Gly Leu Val Ala His Thr Val Tyr Leu 340 345 350 Leu Ile Trp Ala Ile Arg Arg Trp Arg Thr Thr Lys Gly Gly Leu Val 355 360 365 Gly Ile Ser Leu Thr Leu Thr Tyr Pro Ala Leu Met Val Ala Leu Ser 370 375 380 Leu Ala Val Met Phe Val Pro Ala Val His Asn Arg Val Leu Gly Gly 385 390 395 400 Gly Ala Ser Gln Leu Ser Asn Gln Gly Arg Gln Glu Gln Phe Arg Leu 405 410 415 Gly Val Pro Ala Ile Ala Arg Arg Pro Phe Phe Gly Tyr Gly Pro Gly 420 425 430 Glu Gly Ala Gly Ala Val Gly Trp Arg Asn Gln Gln Gly Phe Leu Ser 435 440 445 Ile Asp Ser Gly Phe Leu Ser Val Ala Ala Asp Tyr Gly Leu Leu Gly 450 455 460 Phe Val Ser Leu Tyr Gly Thr Met Ile Thr Leu Met Ile Leu Leu Ala 465 470 475 480 Phe Arg Gly Leu Lys Met Ser Gly Asp Gly Tyr Pro Leu Glu Leu Ala 485 490 495 Val Ala Thr Phe Leu Ala Val Leu Leu Asn Thr Arg Ser Val Leu Ser 500 505 510 Gln Gly Asp Asn Asp Pro Phe Ile Phe Met Thr Leu Gly Leu Gly Ile 515 520 525 Ala Leu Leu Tyr Arg Ser Arg Pro Val Ser Leu Ser Val 530 535 540 61998DNASphingomonas sp. ATCC53159CDS(1)..(1998) 6atg ccg gat atc att gtc aag aat cag acg gag ttg aat gct gca atc 48Met Pro Asp Ile Ile Val Lys Asn Gln Thr Glu Leu Asn Ala Ala Ile 1 5 10 15 gcg gcg gcg aag ggt ggc gaa acc atc aag ctt gcc gcc ggg gtc tac 96Ala Ala Ala Lys Gly Gly Glu Thr Ile Lys Leu Ala Ala Gly Val Tyr 20 25 30 aca gat ctc act gta atg acc aag acg ttt acc agc atg gtg aca att 144Thr Asp Leu Thr Val Met Thr Lys Thr Phe Thr Ser Met Val Thr Ile 35 40 45 gag tcg ctc gac tcg tcg aac ccg gtc aat atc caa aag ctg gtg atc 192Glu Ser Leu Asp Ser Ser Asn Pro Val Asn Ile Gln Lys Leu Val Ile 50 55 60 ggg aac agt agc aac gtt acc gtc aaa aac atg gtc gct gcg acc gat 240Gly Asn Ser Ser Asn Val Thr Val Lys Asn Met Val Ala Ala Thr Asp 65 70 75 80 tac aag ccc gcc gat gac tgg aat cga ctg aat acg atc cag ggt tcg 288Tyr Lys Pro Ala Asp Asp Trp Asn Arg Leu Asn Thr Ile Gln Gly Ser 85 90 95 gcc aac atc gtt ttg gac ggc gtg cgg ttc agc ggc ggc act ggt gac 336Ala Asn Ile Val Leu Asp Gly Val Arg Phe Ser Gly Gly Thr Gly Asp 100 105 110 cct tcg ctc tcg aag ggg gcg ggc ttg ttc gtg cgc aac agc acg tcg 384Pro Ser Leu Ser Lys Gly Ala Gly Leu Phe Val Arg Asn Ser Thr Ser 115 120 125 gtg acg atg cag aat tcg tct atc gac cac ttc ggt ctg ggc ctt gag 432Val Thr Met Gln Asn Ser Ser Ile Asp His Phe Gly Leu Gly Leu Glu 130 135 140 gcc tac aac gtc gat ggc atg gtg gtc cag aac agc agc ttc cac gac 480Ala Tyr Asn Val Asp Gly Met Val Val Gln Asn Ser Ser Phe His Asp 145 150 155 160 aac cgg cgc gat cat acg aac ttc act gag atg aac aat ctt gtc atc 528Asn Arg Arg Asp His Thr Asn Phe Thr Glu Met Asn Asn Leu Val Ile 165 170 175 gac gga aat tcg ttc acg aac ctg ttt ccc gtg ggc acc gaa cat ccc 576Asp Gly Asn Ser Phe Thr Asn Leu Phe Pro Val Gly Thr Glu His Pro 180 185 190 gac gcc att cag ttc ttc acg gcg ggc aag gtc aag ggc aat acc aac 624Asp Ala Ile Gln Phe Phe Thr Ala Gly Lys Val Lys Gly Asn Thr Asn 195 200 205 atc acc atc tcc aat aac gtc atc atg cag ggc gcg ggc tct ggc gcg 672Ile Thr Ile Ser Asn Asn Val Ile Met Gln Gly Ala Gly Ser Gly Ala 210 215 220 caa ggg att ttc atg aat gac gag gcc ggt aat ctt ccc tat gtc aat 720Gln Gly Ile Phe Met Asn Asp Glu Ala Gly Asn Leu Pro Tyr Val Asn 225 230 235 240 gta aac atc aaa aac aat ctt atc tat ctg aat ggt tat tac cac ggt 768Val Asn Ile Lys Asn Asn Leu Ile Tyr Leu Asn Gly Tyr Tyr His Gly

245 250 255 atc aac gtt gtt aac ggc gtt aat gtc aat atc gaa tcc aat agc gtg 816Ile Asn Val Val Asn Gly Val Asn Val Asn Ile Glu Ser Asn Ser Val 260 265 270 ata tcg caa gtg gat ggc aca tca ttt tgg att cgc ctc gac aaa acc 864Ile Ser Gln Val Asp Gly Thr Ser Phe Trp Ile Arg Leu Asp Lys Thr 275 280 285 aat ggc gcg acg atc aag aac aat gtt gcg gac ctg atc acc gtc aca 912Asn Gly Ala Thr Ile Lys Asn Asn Val Ala Asp Leu Ile Thr Val Thr 290 295 300 agc tcc tcg agc aat atc gtg cag aca ggc aat cgt acg ctg acg agt 960Ser Ser Ser Ser Asn Ile Val Gln Thr Gly Asn Arg Thr Leu Thr Ser 305 310 315 320 gac tcg gca acg atc cgc aag atc tat ggc ctc aac gat ggg gct acg 1008Asp Ser Ala Thr Ile Arg Lys Ile Tyr Gly Leu Asn Asp Gly Ala Thr 325 330 335 gcg cgg ctc agc gat ttg atc gtt ccc ggc gtc ggg tac cag ccg ccc 1056Ala Arg Leu Ser Asp Leu Ile Val Pro Gly Val Gly Tyr Gln Pro Pro 340 345 350 gtg tcg agc gct gct gcc gct cag gtg act acc gaa ctg tcg act gcg 1104Val Ser Ser Ala Ala Ala Ala Gln Val Thr Thr Glu Leu Ser Thr Ala 355 360 365 aag gcg gca aat ccg tcg ctg ctg ctc gat ctg tcg ttc agc aac agc 1152Lys Ala Ala Asn Pro Ser Leu Leu Leu Asp Leu Ser Phe Ser Asn Ser 370 375 380 ggc gtc gtg gac ctt tcg cac tgg aat acc ggc cag acg aca aag gcg 1200Gly Val Val Asp Leu Ser His Trp Asn Thr Gly Gln Thr Thr Lys Ala 385 390 395 400 gtg gac gtg tcg gcg gtc gtg ggc agc gcc ttc cac gtc tcg acg ggc 1248Val Asp Val Ser Ala Val Val Gly Ser Ala Phe His Val Ser Thr Gly 405 410 415 acg ggg gtg gaa cta aac cgg agc tat tcg cgg cag att tac gca ttg 1296Thr Gly Val Glu Leu Asn Arg Ser Tyr Ser Arg Gln Ile Tyr Ala Leu 420 425 430 tcg gcg ttc acg ctc agc ttc gac ctc aag cgg gac tcg gct acg gcc 1344Ser Ala Phe Thr Leu Ser Phe Asp Leu Lys Arg Asp Ser Ala Thr Ala 435 440 445 acg gcc ggg caa att ctt ggc atc ttc cag agc tgg tcg gtt tcg ctg 1392Thr Ala Gly Gln Ile Leu Gly Ile Phe Gln Ser Trp Ser Val Ser Leu 450 455 460 cag gcc aat ggg gaa ctg agc ttc acc atg cgc aac gcc gcg ggc gtc 1440Gln Ala Asn Gly Glu Leu Ser Phe Thr Met Arg Asn Ala Ala Gly Val 465 470 475 480 agc cag aca atg gtg acg agc ggc gcc aag ctg ctt gat gct gcc aca 1488Ser Gln Thr Met Val Thr Ser Gly Ala Lys Leu Leu Asp Ala Ala Thr 485 490 495 cac aag atc gcc ctg acc tac gac agc acg cgg aaa acc gcg att ctg 1536His Lys Ile Ala Leu Thr Tyr Asp Ser Thr Arg Lys Thr Ala Ile Leu 500 505 510 tac gta gac ggc atg caa cgc ggc aca gcg acg atg acc ggc acg acc 1584Tyr Val Asp Gly Met Gln Arg Gly Thr Ala Thr Met Thr Gly Thr Thr 515 520 525 cgg ccc gcc gaa tcc tgg ggg ctg tat gtc ggc agc ccg ttc tcg acc 1632Arg Pro Ala Glu Ser Trp Gly Leu Tyr Val Gly Ser Pro Phe Ser Thr 530 535 540 gca ttc agc gga acg gtc ggc gac atc gag atc cgc gat ggc gcg atc 1680Ala Phe Ser Gly Thr Val Gly Asp Ile Glu Ile Arg Asp Gly Ala Ile 545 550 555 560 agc gcc gcc cag gtg cag gcg ctg gtg acc gcg tcg agc gcc agc gcg 1728Ser Ala Ala Gln Val Gln Ala Leu Val Thr Ala Ser Ser Ala Ser Ala 565 570 575 gcg gcg acg gtc aag gac agc ctc gtc acc ggc gcg gcc gcg cag gcc 1776Ala Ala Thr Val Lys Asp Ser Leu Val Thr Gly Ala Ala Ala Gln Ala 580 585 590 gct gcg ctg ctg gcg ggt gcc ggc gcc gct agc acg gca acg ccg ctt 1824Ala Ala Leu Leu Ala Gly Ala Gly Ala Ala Ser Thr Ala Thr Pro Leu 595 600 605 gcg acg gtg gcc acg gtg ggc agt acg ctg tct ata ggt act gcc gcg 1872Ala Thr Val Ala Thr Val Gly Ser Thr Leu Ser Ile Gly Thr Ala Ala 610 615 620 tcc tcg cag atc gcg ctc gtc agc aag atc ggt gtc gac atg atg acc 1920Ser Ser Gln Ile Ala Leu Val Ser Lys Ile Gly Val Asp Met Met Thr 625 630 635 640 gcg ggg gcg atg ggc gca atc cgc agc gcg gcg aca ctg agc gct acg 1968Ala Gly Ala Met Gly Ala Ile Arg Ser Ala Ala Thr Leu Ser Ala Thr 645 650 655 gcg gat cag tac aac ctg tac cgc gcc tga 1998Ala Asp Gln Tyr Asn Leu Tyr Arg Ala 660 665 7665PRTSphingomonas sp. ATCC53159 7Met Pro Asp Ile Ile Val Lys Asn Gln Thr Glu Leu Asn Ala Ala Ile 1 5 10 15 Ala Ala Ala Lys Gly Gly Glu Thr Ile Lys Leu Ala Ala Gly Val Tyr 20 25 30 Thr Asp Leu Thr Val Met Thr Lys Thr Phe Thr Ser Met Val Thr Ile 35 40 45 Glu Ser Leu Asp Ser Ser Asn Pro Val Asn Ile Gln Lys Leu Val Ile 50 55 60 Gly Asn Ser Ser Asn Val Thr Val Lys Asn Met Val Ala Ala Thr Asp 65 70 75 80 Tyr Lys Pro Ala Asp Asp Trp Asn Arg Leu Asn Thr Ile Gln Gly Ser 85 90 95 Ala Asn Ile Val Leu Asp Gly Val Arg Phe Ser Gly Gly Thr Gly Asp 100 105 110 Pro Ser Leu Ser Lys Gly Ala Gly Leu Phe Val Arg Asn Ser Thr Ser 115 120 125 Val Thr Met Gln Asn Ser Ser Ile Asp His Phe Gly Leu Gly Leu Glu 130 135 140 Ala Tyr Asn Val Asp Gly Met Val Val Gln Asn Ser Ser Phe His Asp 145 150 155 160 Asn Arg Arg Asp His Thr Asn Phe Thr Glu Met Asn Asn Leu Val Ile 165 170 175 Asp Gly Asn Ser Phe Thr Asn Leu Phe Pro Val Gly Thr Glu His Pro 180 185 190 Asp Ala Ile Gln Phe Phe Thr Ala Gly Lys Val Lys Gly Asn Thr Asn 195 200 205 Ile Thr Ile Ser Asn Asn Val Ile Met Gln Gly Ala Gly Ser Gly Ala 210 215 220 Gln Gly Ile Phe Met Asn Asp Glu Ala Gly Asn Leu Pro Tyr Val Asn 225 230 235 240 Val Asn Ile Lys Asn Asn Leu Ile Tyr Leu Asn Gly Tyr Tyr His Gly 245 250 255 Ile Asn Val Val Asn Gly Val Asn Val Asn Ile Glu Ser Asn Ser Val 260 265 270 Ile Ser Gln Val Asp Gly Thr Ser Phe Trp Ile Arg Leu Asp Lys Thr 275 280 285 Asn Gly Ala Thr Ile Lys Asn Asn Val Ala Asp Leu Ile Thr Val Thr 290 295 300 Ser Ser Ser Ser Asn Ile Val Gln Thr Gly Asn Arg Thr Leu Thr Ser 305 310 315 320 Asp Ser Ala Thr Ile Arg Lys Ile Tyr Gly Leu Asn Asp Gly Ala Thr 325 330 335 Ala Arg Leu Ser Asp Leu Ile Val Pro Gly Val Gly Tyr Gln Pro Pro 340 345 350 Val Ser Ser Ala Ala Ala Ala Gln Val Thr Thr Glu Leu Ser Thr Ala 355 360 365 Lys Ala Ala Asn Pro Ser Leu Leu Leu Asp Leu Ser Phe Ser Asn Ser 370 375 380 Gly Val Val Asp Leu Ser His Trp Asn Thr Gly Gln Thr Thr Lys Ala 385 390 395 400 Val Asp Val Ser Ala Val Val Gly Ser Ala Phe His Val Ser Thr Gly 405 410 415 Thr Gly Val Glu Leu Asn Arg Ser Tyr Ser Arg Gln Ile Tyr Ala Leu 420 425 430 Ser Ala Phe Thr Leu Ser Phe Asp Leu Lys Arg Asp Ser Ala Thr Ala 435 440 445 Thr Ala Gly Gln Ile Leu Gly Ile Phe Gln Ser Trp Ser Val Ser Leu 450 455 460 Gln Ala Asn Gly Glu Leu Ser Phe Thr Met Arg Asn Ala Ala Gly Val 465 470 475 480 Ser Gln Thr Met Val Thr Ser Gly Ala Lys Leu Leu Asp Ala Ala Thr 485 490 495 His Lys Ile Ala Leu Thr Tyr Asp Ser Thr Arg Lys Thr Ala Ile Leu 500 505 510 Tyr Val Asp Gly Met Gln Arg Gly Thr Ala Thr Met Thr Gly Thr Thr 515 520 525 Arg Pro Ala Glu Ser Trp Gly Leu Tyr Val Gly Ser Pro Phe Ser Thr 530 535 540 Ala Phe Ser Gly Thr Val Gly Asp Ile Glu Ile Arg Asp Gly Ala Ile 545 550 555 560 Ser Ala Ala Gln Val Gln Ala Leu Val Thr Ala Ser Ser Ala Ser Ala 565 570 575 Ala Ala Thr Val Lys Asp Ser Leu Val Thr Gly Ala Ala Ala Gln Ala 580 585 590 Ala Ala Leu Leu Ala Gly Ala Gly Ala Ala Ser Thr Ala Thr Pro Leu 595 600 605 Ala Thr Val Ala Thr Val Gly Ser Thr Leu Ser Ile Gly Thr Ala Ala 610 615 620 Ser Ser Gln Ile Ala Leu Val Ser Lys Ile Gly Val Asp Met Met Thr 625 630 635 640 Ala Gly Ala Met Gly Ala Ile Arg Ser Ala Ala Thr Leu Ser Ala Thr 645 650 655 Ala Asp Gln Tyr Asn Leu Tyr Arg Ala 660 665 8939DNASphingomonas sp. ATCC53159CDS(1)..(939) 8atg cag atg ctc cca acg ccc gat gtc agc ata ctc gtg gtc gct ttc 48Met Gln Met Leu Pro Thr Pro Asp Val Ser Ile Leu Val Val Ala Phe 1 5 10 15 aac tcg acc gag tat atc gaa gac tgc ctg cgc ggc atc gcc gaa gga 96Asn Ser Thr Glu Tyr Ile Glu Asp Cys Leu Arg Gly Ile Ala Glu Gly 20 25 30 gcg ggc aag acc ccc cac gaa gtt ctg ctg atc gac aat ggc gac ggg 144Ala Gly Lys Thr Pro His Glu Val Leu Leu Ile Asp Asn Gly Asp Gly 35 40 45 cga acc gaa gcg ctg gtc cgg cag cgg ttc cac cac gtc cgc atc gtt 192Arg Thr Glu Ala Leu Val Arg Gln Arg Phe His His Val Arg Ile Val 50 55 60 ccc agt gag ggc aat att ggt ttc ggg gcc ggc aat aat cgc ctg gca 240Pro Ser Glu Gly Asn Ile Gly Phe Gly Ala Gly Asn Asn Arg Leu Ala 65 70 75 80 gcg cag gct gcc ggc ccg ctc ctg ctg ctc gtc aac ccc gat gcc att 288Ala Gln Ala Ala Gly Pro Leu Leu Leu Leu Val Asn Pro Asp Ala Ile 85 90 95 ccc cag ccc ggc gca atc gat cag ttg gtc acc ttt gcc aaa cag cat 336Pro Gln Pro Gly Ala Ile Asp Gln Leu Val Thr Phe Ala Lys Gln His 100 105 110 ccc gag gcg gcg gca tgg ggc ggc cgt tcc tac tcg ccc agc ggc gat 384Pro Glu Ala Ala Ala Trp Gly Gly Arg Ser Tyr Ser Pro Ser Gly Asp 115 120 125 cta gaa ccc gca aat ttc atg tcc ctg ccg acg ccc gcc gac ttt ctg 432Leu Glu Pro Ala Asn Phe Met Ser Leu Pro Thr Pro Ala Asp Phe Leu 130 135 140 acg gcg att ttc aac gcg cgt gcg cta cgc agc ggc ggg ctg caa gaa 480Thr Ala Ile Phe Asn Ala Arg Ala Leu Arg Ser Gly Gly Leu Gln Glu 145 150 155 160 ggc gcg acc acc ccc gga gcg gtc gag gtg ttg aat ggc ggc ttc atg 528Gly Ala Thr Thr Pro Gly Ala Val Glu Val Leu Asn Gly Gly Phe Met 165 170 175 atg gta cgc acc gat gtc tgg cag gcg atc ggc ggt ttt gac gag agc 576Met Val Arg Thr Asp Val Trp Gln Ala Ile Gly Gly Phe Asp Glu Ser 180 185 190 ttt ttt ctt tat tcg gaa gag atc gat ctc ttc cag cga atc cgc acg 624Phe Phe Leu Tyr Ser Glu Glu Ile Asp Leu Phe Gln Arg Ile Arg Thr 195 200 205 ttg ggg cac aag gtg ctc gtc gac ccc tcg gtc aaa gtg gta cac aat 672Leu Gly His Lys Val Leu Val Asp Pro Ser Val Lys Val Val His Asn 210 215 220 acg ggg agt ggt cag tcg atg tcc cag aac cgc ctg atg tat ctc acg 720Thr Gly Ser Gly Gln Ser Met Ser Gln Asn Arg Leu Met Tyr Leu Thr 225 230 235 240 acc ggg cgc atg cac tat gcg cga aag cat ttt ggc gca ctc ggc acc 768Thr Gly Arg Met His Tyr Ala Arg Lys His Phe Gly Ala Leu Gly Thr 245 250 255 ctt gcc acc ggg tgc gcg ctt tgg ctg atc gcc gcc aaa tac acg ttg 816Leu Ala Thr Gly Cys Ala Leu Trp Leu Ile Ala Ala Lys Tyr Thr Leu 260 265 270 gtc ggg gcg gca ctc tgg cgc ctg tcg ccg cgg acg ggc acg cga tac 864Val Gly Ala Ala Leu Trp Arg Leu Ser Pro Arg Thr Gly Thr Arg Tyr 275 280 285 aaa gag ctg agc aac ggg tgg cgt gcc gta ttt agc aat cct ggc cga 912Lys Glu Leu Ser Asn Gly Trp Arg Ala Val Phe Ser Asn Pro Gly Arg 290 295 300 tgg tgg agc ggc tat ccg cgt cgc taa 939Trp Trp Ser Gly Tyr Pro Arg Arg 305 310 9312PRTSphingomonas sp. ATCC53159 9Met Gln Met Leu Pro Thr Pro Asp Val Ser Ile Leu Val Val Ala Phe 1 5 10 15 Asn Ser Thr Glu Tyr Ile Glu Asp Cys Leu Arg Gly Ile Ala Glu Gly 20 25 30 Ala Gly Lys Thr Pro His Glu Val Leu Leu Ile Asp Asn Gly Asp Gly 35 40 45 Arg Thr Glu Ala Leu Val Arg Gln Arg Phe His His Val Arg Ile Val 50 55 60 Pro Ser Glu Gly Asn Ile Gly Phe Gly Ala Gly Asn Asn Arg Leu Ala 65 70 75 80 Ala Gln Ala Ala Gly Pro Leu Leu Leu Leu Val Asn Pro Asp Ala Ile 85 90 95 Pro Gln Pro Gly Ala Ile Asp Gln Leu Val Thr Phe Ala Lys Gln His 100 105 110 Pro Glu Ala Ala Ala Trp Gly Gly Arg Ser Tyr Ser Pro Ser Gly Asp 115 120 125 Leu Glu Pro Ala Asn Phe Met Ser Leu Pro Thr Pro Ala Asp Phe Leu 130 135 140 Thr Ala Ile Phe Asn Ala Arg Ala Leu Arg Ser Gly Gly Leu Gln Glu 145 150 155 160 Gly Ala Thr Thr Pro Gly Ala Val Glu Val Leu Asn Gly Gly Phe Met 165 170 175 Met Val Arg Thr Asp Val Trp Gln Ala Ile Gly Gly Phe Asp Glu Ser 180 185 190 Phe Phe Leu Tyr Ser Glu Glu Ile Asp Leu Phe Gln Arg Ile Arg Thr 195 200 205 Leu Gly His Lys Val Leu Val Asp Pro Ser Val Lys Val Val His Asn 210 215 220 Thr Gly Ser Gly Gln Ser Met Ser Gln Asn Arg Leu Met Tyr Leu Thr 225 230 235 240 Thr Gly Arg Met His Tyr Ala Arg Lys His Phe Gly Ala Leu Gly Thr 245 250 255 Leu Ala Thr Gly Cys Ala Leu Trp Leu Ile Ala Ala Lys Tyr Thr Leu 260 265 270 Val Gly Ala Ala Leu Trp Arg Leu Ser Pro Arg Thr Gly Thr Arg Tyr 275 280 285 Lys Glu Leu Ser Asn Gly Trp Arg Ala Val Phe Ser Asn Pro Gly Arg 290 295 300 Trp Trp Ser Gly Tyr Pro Arg Arg 305 310 10972DNASphingomonas sp. ATCC53159CDS(1)..(972) 10atg ctg cac tgc caa cgc tat tgc gga tgc ccg ccc gtc cga ata ggt 48Met Leu His Cys Gln Arg Tyr Cys Gly Cys Pro Pro Val Arg Ile Gly 1 5 10 15 tca agt aga agt ttg tgc cgt gcg caa ttc cgt gcc ggc

ggg gag gtc 96Ser Ser Arg Ser Leu Cys Arg Ala Gln Phe Arg Ala Gly Gly Glu Val 20 25 30 ttc atg aag aaa ttg tac ctg gtt acg gcg gtg gct gcg gcc gcg ctg 144Phe Met Lys Lys Leu Tyr Leu Val Thr Ala Val Ala Ala Ala Ala Leu 35 40 45 gcc gtc tcc gga tgt ggc ggc aag ggc ggc aag ctc gac aag ggg cag 192Ala Val Ser Gly Cys Gly Gly Lys Gly Gly Lys Leu Asp Lys Gly Gln 50 55 60 gtg gtc gcc agc gtc gat ggc gaa gaa atc acc gtc ttc gag ctg aat 240Val Val Ala Ser Val Asp Gly Glu Glu Ile Thr Val Phe Glu Leu Asn 65 70 75 80 gcc gaa ctg cag gcc tcc cag gta ccc ccg ggg acc gat cgc aag ctg 288Ala Glu Leu Gln Ala Ser Gln Val Pro Pro Gly Thr Asp Arg Lys Leu 85 90 95 gcc gag cag ctg gcg ctg cag cgc atc atc gag cgc aag atc ctc gcc 336Ala Glu Gln Leu Ala Leu Gln Arg Ile Ile Glu Arg Lys Ile Leu Ala 100 105 110 aag gtc gcc cgc gag cag aag ctg gac aag acg cct gcc ttc ctg atc 384Lys Val Ala Arg Glu Gln Lys Leu Asp Lys Thr Pro Ala Phe Leu Ile 115 120 125 cag gag cgc cgg gcc gac gag ctg atc ctc acc gcc atg ctg cgc gac 432Gln Glu Arg Arg Ala Asp Glu Leu Ile Leu Thr Ala Met Leu Arg Asp 130 135 140 aag atc gcc ggc ggc atc gcc cag ccg acc gat gcc gag atc gag aaa 480Lys Ile Ala Gly Gly Ile Ala Gln Pro Thr Asp Ala Glu Ile Glu Lys 145 150 155 160 tat cag gcc gcg cat ccg gag cgg ttc gcg cag cgc aag atc tac gcg 528Tyr Gln Ala Ala His Pro Glu Arg Phe Ala Gln Arg Lys Ile Tyr Ala 165 170 175 atc gat cag gtc gtc ttc gct ccg ccg agc tcg gcc gca aag ctc aag 576Ile Asp Gln Val Val Phe Ala Pro Pro Ser Ser Ala Ala Lys Leu Lys 180 185 190 caa ttc gcg ccg ctg aag acg ctg gac cag cta acc gcc aag ctc tcg 624Gln Phe Ala Pro Leu Lys Thr Leu Asp Gln Leu Thr Ala Lys Leu Ser 195 200 205 gcg gac aat gtc cag ttc cgt cgc gcg ccg tcg cag atc gac acc gct 672Ala Asp Asn Val Gln Phe Arg Arg Ala Pro Ser Gln Ile Asp Thr Ala 210 215 220 gcg ctg ccg ccg gaa atc gct gcc aag atc gcg tcg ctg ccg gca cag 720Ala Leu Pro Pro Glu Ile Ala Ala Lys Ile Ala Ser Leu Pro Ala Gln 225 230 235 240 gag atg ttc atc ctg ccg acc cag cag gga ctg acc gcg aat atc atc 768Glu Met Phe Ile Leu Pro Thr Gln Gln Gly Leu Thr Ala Asn Ile Ile 245 250 255 acg tcg acc acg gtg ctg ccg gtg ccg gcc gac cag gcg cgc gag atc 816Thr Ser Thr Thr Val Leu Pro Val Pro Ala Asp Gln Ala Arg Glu Ile 260 265 270 gcg ctc agc ggg ctg cgt acc gag cgc ttc ggc aag gcg gct gac gca 864Ala Leu Ser Gly Leu Arg Thr Glu Arg Phe Gly Lys Ala Ala Asp Ala 275 280 285 cag ctc aac gac cgc ctg aag aag gcg cgg gaa acc gtg aaa tat cag 912Gln Leu Asn Asp Arg Leu Lys Lys Ala Arg Glu Thr Val Lys Tyr Gln 290 295 300 gcc ggc tac agc gca ccg ccg cag ctt cgc ggc agc ggc gca acg ccg 960Ala Gly Tyr Ser Ala Pro Pro Gln Leu Arg Gly Ser Gly Ala Thr Pro 305 310 315 320 gcg ggg aac tga 972Ala Gly Asn 11323PRTSphingomonas sp. ATCC53159 11Met Leu His Cys Gln Arg Tyr Cys Gly Cys Pro Pro Val Arg Ile Gly 1 5 10 15 Ser Ser Arg Ser Leu Cys Arg Ala Gln Phe Arg Ala Gly Gly Glu Val 20 25 30 Phe Met Lys Lys Leu Tyr Leu Val Thr Ala Val Ala Ala Ala Ala Leu 35 40 45 Ala Val Ser Gly Cys Gly Gly Lys Gly Gly Lys Leu Asp Lys Gly Gln 50 55 60 Val Val Ala Ser Val Asp Gly Glu Glu Ile Thr Val Phe Glu Leu Asn 65 70 75 80 Ala Glu Leu Gln Ala Ser Gln Val Pro Pro Gly Thr Asp Arg Lys Leu 85 90 95 Ala Glu Gln Leu Ala Leu Gln Arg Ile Ile Glu Arg Lys Ile Leu Ala 100 105 110 Lys Val Ala Arg Glu Gln Lys Leu Asp Lys Thr Pro Ala Phe Leu Ile 115 120 125 Gln Glu Arg Arg Ala Asp Glu Leu Ile Leu Thr Ala Met Leu Arg Asp 130 135 140 Lys Ile Ala Gly Gly Ile Ala Gln Pro Thr Asp Ala Glu Ile Glu Lys 145 150 155 160 Tyr Gln Ala Ala His Pro Glu Arg Phe Ala Gln Arg Lys Ile Tyr Ala 165 170 175 Ile Asp Gln Val Val Phe Ala Pro Pro Ser Ser Ala Ala Lys Leu Lys 180 185 190 Gln Phe Ala Pro Leu Lys Thr Leu Asp Gln Leu Thr Ala Lys Leu Ser 195 200 205 Ala Asp Asn Val Gln Phe Arg Arg Ala Pro Ser Gln Ile Asp Thr Ala 210 215 220 Ala Leu Pro Pro Glu Ile Ala Ala Lys Ile Ala Ser Leu Pro Ala Gln 225 230 235 240 Glu Met Phe Ile Leu Pro Thr Gln Gln Gly Leu Thr Ala Asn Ile Ile 245 250 255 Thr Ser Thr Thr Val Leu Pro Val Pro Ala Asp Gln Ala Arg Glu Ile 260 265 270 Ala Leu Ser Gly Leu Arg Thr Glu Arg Phe Gly Lys Ala Ala Asp Ala 275 280 285 Gln Leu Asn Asp Arg Leu Lys Lys Ala Arg Glu Thr Val Lys Tyr Gln 290 295 300 Ala Gly Tyr Ser Ala Pro Pro Gln Leu Arg Gly Ser Gly Ala Thr Pro 305 310 315 320 Ala Gly Asn 121047DNASphingomonas sp. ATCC53159CDS(1)..(1044) 12atg gca gaa gcg aac gcg gta gat gga aag gcc tcc aag ccg ctg aaa 48 Met Ala Glu Ala Asn Ala Val Asp Gly Lys Ala Ser Lys Pro Leu Lys1 5 10 15 atg tgc ctt gca gcg tcg ggc ggc ggc cat ctc cgg caa atc ctc gat 96 Met Cys Leu Ala Ala Ser Gly Gly Gly His Leu Arg Gln Ile Leu Asp 20 25 30 ctg gaa tcg gtg tgg cgc gaa cac gat tat ttc ttc gtt act gaa gat 144 Leu Glu Ser Val Trp Arg Glu His Asp Tyr Phe Phe Val Thr Glu Asp 35 40 45 acc gcg ctc ggc cgg agc ctt gcc gaa aaa cat ccc gtc gaa ctg gtg 192 Thr Ala Leu Gly Arg Ser Leu Ala Glu Lys His Pro Val Glu Leu Val 50 55 60 gag cac tat gcg ctc ggc cag gcc aag ctg ggc cat ccc ttg cgc atg 240 Glu His Tyr Ala Leu Gly Gln Ala Lys Leu Gly His Pro Leu Arg Met65 70 75 80ctg ggc ggc gca tgg cgc aac ctg cgc cag agc ctt tcg atc ctg cgc 288 Leu Gly Gly Ala Trp Arg Asn Leu Arg Gln Ser Leu Ser Ile Leu Arg 85 90 95 cgg cac aag ccg gat gtg gtg att tcc acc ggc gcg ggc gca gtc tat 336 Arg His Lys Pro Asp Val Val Ile Ser Thr Gly Ala Gly Ala Val Tyr 100 105 110 ttc acc gcg ctg ctc gcc aaa ctg tcg ggc gcc aag ttc gtc cat atc 384 Phe Thr Ala Leu Leu Ala Lys Leu Ser Gly Ala Lys Phe Val His Ile 115 120 125 gaa agc ttc gcg cgc ttc gac cac ccg tct gcc ttc ggc aag atg gtg 432 Glu Ser Phe Ala Arg Phe Asp His Pro Ser Ala Phe Gly Lys Met Val 130 135 140 aag ggc atc gcg acg gtg acg atc gtc cag tcg gcg gcg ctg aaa gaa 480 Lys Gly Ile Ala Thr Val Thr Ile Val Gln Ser Ala Ala Leu Lys Glu145 150 155 160acc tgg cct gat gcc gag ctg ttc gat ccg ttc cgc ctg ctc gat aca 528 Thr Trp Pro Asp Ala Glu Leu Phe Asp Pro Phe Arg Leu Leu Asp Thr 165 170 175 ccg cgc ccg ccc aag cag gcg cta atc ttc gcg acg gtc ggc gcc acc 576 Pro Arg Pro Pro Lys Gln Ala Leu Ile Phe Ala Thr Val Gly Ala Thr 180 185 190 ctg ccc ttc ccg cgg ctg gtg cag gca gtg ctc gac ctg aag cgc gcc 624 Leu Pro Phe Pro Arg Leu Val Gln Ala Val Leu Asp Leu Lys Arg Ala 195 200 205 ggc ggg ctg ccg ggc aag ctg atc ctg caa tat ggc gac cag gac ctg 672 Gly Gly Leu Pro Gly Lys Leu Ile Leu Gln Tyr Gly Asp Gln Asp Leu 210 215 220 ccc gat ccc ggc atc ccc gac gtc gag atc cgc cgt acc atc ccg ttc 720 Pro Asp Pro Gly Ile Pro Asp Val Glu Ile Arg Arg Thr Ile Pro Phe225 230 235 240gac gat ctg cag ctg ctg ctg cgc gat gcg gat atg gtg ata tgc cac 768 Asp Asp Leu Gln Leu Leu Leu Arg Asp Ala Asp Met Val Ile Cys His 245 250 255 ggc ggc acc gga tcg ctg gtc acg gcg ctg cgc gcc ggc tgc cgg gtc 816 Gly Gly Thr Gly Ser Leu Val Thr Ala Leu Arg Ala Gly Cys Arg Val 260 265 270 gtc gcc ttt ccg cgc cgc cac gat ctg ggc gag cat tat gac gat cac 864 Val Ala Phe Pro Arg Arg His Asp Leu Gly Glu His Tyr Asp Asp His 275 280 285 cag gaa gag atc gcc cag acc ttc gcc gac cgg ggc ctg ctc cag gcg 912 Gln Glu Glu Ile Ala Gln Thr Phe Ala Asp Arg Gly Leu Leu Gln Ala 290 295 300 gtg cgc gac gag cgc cag ctc ggc gcc gct gtg gaa gcg gcc aag gca 960 Val Arg Asp Glu Arg Gln Leu Gly Ala Ala Val Glu Ala Ala Lys Ala305 310 315 320acc gag ccg cag ctg gcg acc acc gac cac acg gcc ctc gcg gcg cgg 1008 Thr Glu Pro Gln Leu Ala Thr Thr Asp His Thr Ala Leu Ala Ala Arg 325 330 335 ctg cgc cag ctg ctg gcg cag tgg agt gcc aag cga tga 1047 Leu Arg Gln Leu Leu Ala Gln Trp Ser Ala Lys Arg 340 345 13348PRTSphingomonas sp. ATCC53159 13Met Ala Glu Ala Asn Ala Val Asp Gly Lys Ala Ser Lys Pro Leu Lys 1 5 10 15 Met Cys Leu Ala Ala Ser Gly Gly Gly His Leu Arg Gln Ile Leu Asp 20 25 30 Leu Glu Ser Val Trp Arg Glu His Asp Tyr Phe Phe Val Thr Glu Asp 35 40 45 Thr Ala Leu Gly Arg Ser Leu Ala Glu Lys His Pro Val Glu Leu Val 50 55 60 Glu His Tyr Ala Leu Gly Gln Ala Lys Leu Gly His Pro Leu Arg Met 65 70 75 80 Leu Gly Gly Ala Trp Arg Asn Leu Arg Gln Ser Leu Ser Ile Leu Arg 85 90 95 Arg His Lys Pro Asp Val Val Ile Ser Thr Gly Ala Gly Ala Val Tyr 100 105 110 Phe Thr Ala Leu Leu Ala Lys Leu Ser Gly Ala Lys Phe Val His Ile 115 120 125 Glu Ser Phe Ala Arg Phe Asp His Pro Ser Ala Phe Gly Lys Met Val 130 135 140 Lys Gly Ile Ala Thr Val Thr Ile Val Gln Ser Ala Ala Leu Lys Glu 145 150 155 160 Thr Trp Pro Asp Ala Glu Leu Phe Asp Pro Phe Arg Leu Leu Asp Thr 165 170 175 Pro Arg Pro Pro Lys Gln Ala Leu Ile Phe Ala Thr Val Gly Ala Thr 180 185 190 Leu Pro Phe Pro Arg Leu Val Gln Ala Val Leu Asp Leu Lys Arg Ala 195 200 205 Gly Gly Leu Pro Gly Lys Leu Ile Leu Gln Tyr Gly Asp Gln Asp Leu 210 215 220 Pro Asp Pro Gly Ile Pro Asp Val Glu Ile Arg Arg Thr Ile Pro Phe 225 230 235 240 Asp Asp Leu Gln Leu Leu Leu Arg Asp Ala Asp Met Val Ile Cys His 245 250 255 Gly Gly Thr Gly Ser Leu Val Thr Ala Leu Arg Ala Gly Cys Arg Val 260 265 270 Val Ala Phe Pro Arg Arg His Asp Leu Gly Glu His Tyr Asp Asp His 275 280 285 Gln Glu Glu Ile Ala Gln Thr Phe Ala Asp Arg Gly Leu Leu Gln Ala 290 295 300 Val Arg Asp Glu Arg Gln Leu Gly Ala Ala Val Glu Ala Ala Lys Ala 305 310 315 320 Thr Glu Pro Gln Leu Ala Thr Thr Asp His Thr Ala Leu Ala Ala Arg 325 330 335 Leu Arg Gln Leu Leu Ala Gln Trp Ser Ala Lys Arg 340 345 14867DNASphingomonas sp. ATCC53159CDS(1)..(867) 14atg agc acg ccc cgg atc agc gtc gtc atc ccg cac tat aac gat ccg 48Met Ser Thr Pro Arg Ile Ser Val Val Ile Pro His Tyr Asn Asp Pro 1 5 10 15 caa tcc ttg cgg ctc tgc ctg gat gcg ctg gag cgg cag acg atc ggt 96Gln Ser Leu Arg Leu Cys Leu Asp Ala Leu Glu Arg Gln Thr Ile Gly 20 25 30 cgc gac gcg ttc gag atc atc gtc ggc gac aac aat tcg ccc tgt ggg 144Arg Asp Ala Phe Glu Ile Ile Val Gly Asp Asn Asn Ser Pro Cys Gly 35 40 45 ctc gcg gcg gtg gag gcg gcg gtc gcc gga cgt gcg cgg atc gtg acc 192Leu Ala Ala Val Glu Ala Ala Val Ala Gly Arg Ala Arg Ile Val Thr 50 55 60 att ctg gaa aag ggg gcg ggc ccc gcg cgc aac ggg gcg gca gcc gca 240Ile Leu Glu Lys Gly Ala Gly Pro Ala Arg Asn Gly Ala Ala Ala Ala 65 70 75 80 gcg cgt ggc gag atc ctc gcc ttt acc gac agt gac tgc gtg gtg gag 288Ala Arg Gly Glu Ile Leu Ala Phe Thr Asp Ser Asp Cys Val Val Glu 85 90 95 ccc ggc tgg ctg gcg ggc ggc acg acc agg gtc gcg cct ggc cgt ttc 336Pro Gly Trp Leu Ala Gly Gly Thr Thr Arg Val Ala Pro Gly Arg Phe 100 105 110 atc ggc ggg cac atg tat gtg cgc aag ccc gaa ggg ccg ccg aac ggc 384Ile Gly Gly His Met Tyr Val Arg Lys Pro Glu Gly Pro Pro Asn Gly 115 120 125 gcc gag gcg ctg gag atg gcg ctg gcg ttc gac aat gaa ggc tat gtg 432Ala Glu Ala Leu Glu Met Ala Leu Ala Phe Asp Asn Glu Gly Tyr Val 130 135 140 cgg cgc acc cag ttc acg gtc acc gca aac ctg ttc gtg atg cgc gcc 480Arg Arg Thr Gln Phe Thr Val Thr Ala Asn Leu Phe Val Met Arg Ala 145 150 155 160 gat ttc gaa cgg gtc ggc ggc ttc cgc gtt ggc gtg tcc gag gat ctg 528Asp Phe Glu Arg Val Gly Gly Phe Arg Val Gly Val Ser Glu Asp Leu 165 170 175 gaa tgg tgc cac cgg gcg atc gcc agc ggc ctc acc atc aac tat gca 576Glu Trp Cys His Arg Ala Ile Ala Ser Gly Leu Thr Ile Asn Tyr Ala 180 185 190 ccg gat gca tcg gtg ggc cac ccg ccc cgg ccc gac tgg tcg gcc ctg 624Pro Asp Ala Ser Val Gly His Pro Pro Arg Pro Asp Trp Ser Ala Leu 195 200 205 ctg gtg aag acg cgg cgc atc cag cgc gaa ctc tat ctg ttc aac atc 672Leu Val Lys Thr Arg Arg Ile Gln Arg Glu Leu Tyr Leu Phe Asn Ile 210 215 220 gag cgg ccg aag ggc agg ctg cgc tgg ctg gtc cgt tcc gtg gcg caa 720Glu Arg Pro Lys Gly Arg Leu Arg Trp Leu Val Arg Ser Val Ala Gln 225 230 235 240 ccg gcg atg atc cca cag gac gtg gcc aag atc ctg cgc aca ccg ggt 768Pro Ala Met Ile Pro Gln Asp Val Ala Lys Ile Leu Arg Thr Pro Gly 245 250 255 acc aag ggc gcg cgc ctc gct gcg gtc acc acg ctg gtc cgg ctg cgg 816Thr Lys Gly Ala Arg Leu Ala Ala Val Thr Thr Leu Val Arg Leu Arg 260 265 270 ctg tgg cgc ggc ggc gcc ggc ttg ttg cag ttg ctc ggc cgc gac atc 864Leu Trp Arg Gly Gly Ala Gly Leu Leu Gln Leu Leu Gly Arg Asp Ile 275 280 285 tga 867 15288PRTSphingomonas sp. ATCC53159 15Met Ser Thr Pro Arg Ile Ser Val Val Ile Pro His Tyr Asn Asp Pro 1 5 10

15 Gln Ser Leu Arg Leu Cys Leu Asp Ala Leu Glu Arg Gln Thr Ile Gly 20 25 30 Arg Asp Ala Phe Glu Ile Ile Val Gly Asp Asn Asn Ser Pro Cys Gly 35 40 45 Leu Ala Ala Val Glu Ala Ala Val Ala Gly Arg Ala Arg Ile Val Thr 50 55 60 Ile Leu Glu Lys Gly Ala Gly Pro Ala Arg Asn Gly Ala Ala Ala Ala 65 70 75 80 Ala Arg Gly Glu Ile Leu Ala Phe Thr Asp Ser Asp Cys Val Val Glu 85 90 95 Pro Gly Trp Leu Ala Gly Gly Thr Thr Arg Val Ala Pro Gly Arg Phe 100 105 110 Ile Gly Gly His Met Tyr Val Arg Lys Pro Glu Gly Pro Pro Asn Gly 115 120 125 Ala Glu Ala Leu Glu Met Ala Leu Ala Phe Asp Asn Glu Gly Tyr Val 130 135 140 Arg Arg Thr Gln Phe Thr Val Thr Ala Asn Leu Phe Val Met Arg Ala 145 150 155 160 Asp Phe Glu Arg Val Gly Gly Phe Arg Val Gly Val Ser Glu Asp Leu 165 170 175 Glu Trp Cys His Arg Ala Ile Ala Ser Gly Leu Thr Ile Asn Tyr Ala 180 185 190 Pro Asp Ala Ser Val Gly His Pro Pro Arg Pro Asp Trp Ser Ala Leu 195 200 205 Leu Val Lys Thr Arg Arg Ile Gln Arg Glu Leu Tyr Leu Phe Asn Ile 210 215 220 Glu Arg Pro Lys Gly Arg Leu Arg Trp Leu Val Arg Ser Val Ala Gln 225 230 235 240 Pro Ala Met Ile Pro Gln Asp Val Ala Lys Ile Leu Arg Thr Pro Gly 245 250 255 Thr Lys Gly Ala Arg Leu Ala Ala Val Thr Thr Leu Val Arg Leu Arg 260 265 270 Leu Trp Arg Gly Gly Ala Gly Leu Leu Gln Leu Leu Gly Arg Asp Ile 275 280 285 161389DNASphingomonas sp. ATCC53159CDS(1)..(1389) 16gtg gct gta ggt tcc gcg ctg aga ttc ttg tgg ccg ttc ggc cgt cga 48Val Ala Val Gly Ser Ala Leu Arg Phe Leu Trp Pro Phe Gly Arg Arg 1 5 10 15 gaa gaa cct gaa gaa gag ggc tat ttc ccg ctg act gcg aca gtg gtg 96Glu Glu Pro Glu Glu Glu Gly Tyr Phe Pro Leu Thr Ala Thr Val Val 20 25 30 ccg cat cgc gat gcc cat agc ggc cgt ggt cgc ccg gat ttc cca acc 144Pro His Arg Asp Ala His Ser Gly Arg Gly Arg Pro Asp Phe Pro Thr 35 40 45 ttc cgt gcc tcc gcg ctg gac cgc ccg ctg gat cgc cgc cgc gac gag 192Phe Arg Ala Ser Ala Leu Asp Arg Pro Leu Asp Arg Arg Arg Asp Glu 50 55 60 cgc cgc gag atc acg cgc gcc cgg ttc gcg ctg gcg acc ttc ttc aca 240Arg Arg Glu Ile Thr Arg Ala Arg Phe Ala Leu Ala Thr Phe Phe Thr 65 70 75 80 ccc acc cag ccg gtg gcc gat cgg tcg agc ttc gcc ggg cgc ctc ggc 288Pro Thr Gln Pro Val Ala Asp Arg Ser Ser Phe Ala Gly Arg Leu Gly 85 90 95 gtg ctg gcg cgc cta atc tcc tcg atc gag agc cag cgc agc cat gtc 336Val Leu Ala Arg Leu Ile Ser Ser Ile Glu Ser Gln Arg Ser His Val 100 105 110 gtg ctc tat ggc gag cgc ggc atc ggc aag acc tcg ctg ctc cac gtg 384Val Leu Tyr Gly Glu Arg Gly Ile Gly Lys Thr Ser Leu Leu His Val 115 120 125 ctg acc gat gtc gcc cgc gaa tcc agc tat atc gtc agc tat gcg acc 432Leu Thr Asp Val Ala Arg Glu Ser Ser Tyr Ile Val Ser Tyr Ala Thr 130 135 140 tgc ggt gcg aac gcg aat ttc agc gat gtc ttc cgc gcc gtg ctg gaa 480Cys Gly Ala Asn Ala Asn Phe Ser Asp Val Phe Arg Ala Val Leu Glu 145 150 155 160 gac gtg ccg ctg ctg ttc cat cgc ggc gtg gcg ccc aac gcc ggc gag 528Asp Val Pro Leu Leu Phe His Arg Gly Val Ala Pro Asn Ala Gly Glu 165 170 175 gcg gag agc ggc ggc aac ttg gcc gac cgc ctg ccg acg ggc agc ttc 576Ala Glu Ser Gly Gly Asn Leu Ala Asp Arg Leu Pro Thr Gly Ser Phe 180 185 190 ggg ccc ggc gaa ctg gcc gac ctg tgc gcc gac atc acg ggc aca cgc 624Gly Pro Gly Glu Leu Ala Asp Leu Cys Ala Asp Ile Thr Gly Thr Arg 195 200 205 gtg ctg atc atc ctc gac gaa tat gat cgc gtc agc gat tcc gcc ttc 672Val Leu Ile Ile Leu Asp Glu Tyr Asp Arg Val Ser Asp Ser Ala Phe 210 215 220 cgt cag cag gtc gcc gag ctg atc aag aac ctg tcg gac cgt tcg gcg 720Arg Gln Gln Val Ala Glu Leu Ile Lys Asn Leu Ser Asp Arg Ser Ala 225 230 235 240 cgc gtc cag ctg gtg atc gcg ggc gtc gcc tcg aac ctg cag gag ctg 768Arg Val Gln Leu Val Ile Ala Gly Val Ala Ser Asn Leu Gln Glu Leu 245 250 255 atc ggt tat gcg ccg tcg atc cgc cgc aac gtc atc ggc ctg ccg atg 816Ile Gly Tyr Ala Pro Ser Ile Arg Arg Asn Val Ile Gly Leu Pro Met 260 265 270 ccc cgg ctg gag gaa tcg gag gtg cag gag atg atc gcg ctc ggc gaa 864Pro Arg Leu Glu Glu Ser Glu Val Gln Glu Met Ile Ala Leu Gly Glu 275 280 285 acc gcc tcg ggc gtt cgc ttc gat ccg gac ctg act cac atg atc cac 912Thr Ala Ser Gly Val Arg Phe Asp Pro Asp Leu Thr His Met Ile His 290 295 300 ctg ctc gcg ctg ggg tcg ccc tat ttc gcg cgg ctg ctg tgc cac cat 960Leu Leu Ala Leu Gly Ser Pro Tyr Phe Ala Arg Leu Leu Cys His His 305 310 315 320 tcc gcg ctg gaa gcc ctg gac cag ggc cgc ctc acg gtc gac gcc ggg 1008Ser Ala Leu Glu Ala Leu Asp Gln Gly Arg Leu Thr Val Asp Ala Gly 325 330 335 cat ctg cgt cgt gcg ctc gac cag gcg atc ctt gag atc gag ggc cgc 1056His Leu Arg Arg Ala Leu Asp Gln Ala Ile Leu Glu Ile Glu Gly Arg 340 345 350 atg ccg ccg cgc gcg gtg atc gag atg cgc aag ttc gtc ggc ggc cgc 1104Met Pro Pro Arg Ala Val Ile Glu Met Arg Lys Phe Val Gly Gly Arg 355 360 365 tac gat cca ctc gtc gcg gcg ctg ggc gag gcc tcg cgc tcg gcg gat 1152Tyr Asp Pro Leu Val Ala Ala Leu Gly Glu Ala Ser Arg Ser Ala Asp 370 375 380 ggc tgg ttc agc ggc caa gcc gtg gtg gat ctg ctg ccg ggc gcg cac 1200Gly Trp Phe Ser Gly Gln Ala Val Val Asp Leu Leu Pro Gly Ala His 385 390 395 400 atc acg gcg gcg cag gtc gag cag gag ctg ggc gag ctc acc ggt caa 1248Ile Thr Ala Ala Gln Val Glu Gln Glu Leu Gly Glu Leu Thr Gly Gln 405 410 415 ctc ggc ctc gaa tcc gaa acg cag gac ggc gat tgc cgc ttc cgc ttc 1296Leu Gly Leu Glu Ser Glu Thr Gln Asp Gly Asp Cys Arg Phe Arg Phe 420 425 430 acc gac gat acg ctg ccg gtc tat ctg tgg ctg atg atc ggc cgc ctg 1344Thr Asp Asp Thr Leu Pro Val Tyr Leu Trp Leu Met Ile Gly Arg Leu 435 440 445 cgg ctc gac agc ggc acg ctg gaa gac gcg ctg gcc acc gtc tga 1389Arg Leu Asp Ser Gly Thr Leu Glu Asp Ala Leu Ala Thr Val 450 455 460 17462PRTSphingomonas sp. ATCC53159 17Val Ala Val Gly Ser Ala Leu Arg Phe Leu Trp Pro Phe Gly Arg Arg 1 5 10 15 Glu Glu Pro Glu Glu Glu Gly Tyr Phe Pro Leu Thr Ala Thr Val Val 20 25 30 Pro His Arg Asp Ala His Ser Gly Arg Gly Arg Pro Asp Phe Pro Thr 35 40 45 Phe Arg Ala Ser Ala Leu Asp Arg Pro Leu Asp Arg Arg Arg Asp Glu 50 55 60 Arg Arg Glu Ile Thr Arg Ala Arg Phe Ala Leu Ala Thr Phe Phe Thr 65 70 75 80 Pro Thr Gln Pro Val Ala Asp Arg Ser Ser Phe Ala Gly Arg Leu Gly 85 90 95 Val Leu Ala Arg Leu Ile Ser Ser Ile Glu Ser Gln Arg Ser His Val 100 105 110 Val Leu Tyr Gly Glu Arg Gly Ile Gly Lys Thr Ser Leu Leu His Val 115 120 125 Leu Thr Asp Val Ala Arg Glu Ser Ser Tyr Ile Val Ser Tyr Ala Thr 130 135 140 Cys Gly Ala Asn Ala Asn Phe Ser Asp Val Phe Arg Ala Val Leu Glu 145 150 155 160 Asp Val Pro Leu Leu Phe His Arg Gly Val Ala Pro Asn Ala Gly Glu 165 170 175 Ala Glu Ser Gly Gly Asn Leu Ala Asp Arg Leu Pro Thr Gly Ser Phe 180 185 190 Gly Pro Gly Glu Leu Ala Asp Leu Cys Ala Asp Ile Thr Gly Thr Arg 195 200 205 Val Leu Ile Ile Leu Asp Glu Tyr Asp Arg Val Ser Asp Ser Ala Phe 210 215 220 Arg Gln Gln Val Ala Glu Leu Ile Lys Asn Leu Ser Asp Arg Ser Ala 225 230 235 240 Arg Val Gln Leu Val Ile Ala Gly Val Ala Ser Asn Leu Gln Glu Leu 245 250 255 Ile Gly Tyr Ala Pro Ser Ile Arg Arg Asn Val Ile Gly Leu Pro Met 260 265 270 Pro Arg Leu Glu Glu Ser Glu Val Gln Glu Met Ile Ala Leu Gly Glu 275 280 285 Thr Ala Ser Gly Val Arg Phe Asp Pro Asp Leu Thr His Met Ile His 290 295 300 Leu Leu Ala Leu Gly Ser Pro Tyr Phe Ala Arg Leu Leu Cys His His 305 310 315 320 Ser Ala Leu Glu Ala Leu Asp Gln Gly Arg Leu Thr Val Asp Ala Gly 325 330 335 His Leu Arg Arg Ala Leu Asp Gln Ala Ile Leu Glu Ile Glu Gly Arg 340 345 350 Met Pro Pro Arg Ala Val Ile Glu Met Arg Lys Phe Val Gly Gly Arg 355 360 365 Tyr Asp Pro Leu Val Ala Ala Leu Gly Glu Ala Ser Arg Ser Ala Asp 370 375 380 Gly Trp Phe Ser Gly Gln Ala Val Val Asp Leu Leu Pro Gly Ala His 385 390 395 400 Ile Thr Ala Ala Gln Val Glu Gln Glu Leu Gly Glu Leu Thr Gly Gln 405 410 415 Leu Gly Leu Glu Ser Glu Thr Gln Asp Gly Asp Cys Arg Phe Arg Phe 420 425 430 Thr Asp Asp Thr Leu Pro Val Tyr Leu Trp Leu Met Ile Gly Arg Leu 435 440 445 Arg Leu Asp Ser Gly Thr Leu Glu Asp Ala Leu Ala Thr Val 450 455 460 181299DNASphingomonas sp. ATCC53159CDS(1)..(1299) 18atg aag ccg aga ccc ggg gga acc ttt atg caa gta aat ttc aat cga 48Met Lys Pro Arg Pro Gly Gly Thr Phe Met Gln Val Asn Phe Asn Arg 1 5 10 15 cag gct cgc aag ctc ggt gcc ggc aat gcg ctc gcg cgg ggg ggg ccc 96Gln Ala Arg Lys Leu Gly Ala Gly Asn Ala Leu Ala Arg Gly Gly Pro 20 25 30 gtg ctt gcg ctg ctt gcg acc gcg gca tgg aca caa cct gcg ctg gcg 144Val Leu Ala Leu Leu Ala Thr Ala Ala Trp Thr Gln Pro Ala Leu Ala 35 40 45 cag cga cag gca ttt gag tcc cgc ccc tcc ggt agc gag cga cag gtc 192Gln Arg Gln Ala Phe Glu Ser Arg Pro Ser Gly Ser Glu Arg Gln Val 50 55 60 gat att cgc gcg acg ggg tcg ctg gaa tat gac gac aac gtc gtg ctg 240Asp Ile Arg Ala Thr Gly Ser Leu Glu Tyr Asp Asp Asn Val Val Leu 65 70 75 80 aac gac cag cgg atc acg gac ggc gcg cgt ggc gat gtg atc gca tcg 288Asn Asp Gln Arg Ile Thr Asp Gly Ala Arg Gly Asp Val Ile Ala Ser 85 90 95 ccc ggg ctg gac gtg acc cta gtt ctg ccc cgc gcc acc ggg cag ctc 336Pro Gly Leu Asp Val Thr Leu Val Leu Pro Arg Ala Thr Gly Gln Leu 100 105 110 tac ctc acc ggc aat gtc gga tat cgc ttt tac aag cga tat acc aac 384Tyr Leu Thr Gly Asn Val Gly Tyr Arg Phe Tyr Lys Arg Tyr Thr Asn 115 120 125 ttt aac cgc gag cag atc tcg ctc acc ggc ggc gca gat cag cgg ttc 432Phe Asn Arg Glu Gln Ile Ser Leu Thr Gly Gly Ala Asp Gln Arg Phe 130 135 140 gcc tcc tgc gtc gtg cac ggg gaa gtc ggc tat cag cgc cac ctc acc 480Ala Ser Cys Val Val His Gly Glu Val Gly Tyr Gln Arg His Leu Thr 145 150 155 160 gac ctg tcc agc atc ttg atc cag gac acc acg cct gcg ctc aac aac 528Asp Leu Ser Ser Ile Leu Ile Gln Asp Thr Thr Pro Ala Leu Asn Asn 165 170 175 acc gaa gag gcc cgg cag tac acc gcg gat atc ggc tgc ggc gcg acc 576Thr Glu Glu Ala Arg Gln Tyr Thr Ala Asp Ile Gly Cys Gly Ala Thr 180 185 190 tac ggc ctg cgg cct gcc gtt tcc tac acc cgc aac gaa gtg cgc aac 624Tyr Gly Leu Arg Pro Ala Val Ser Tyr Thr Arg Asn Glu Val Arg Asn 195 200 205 agc ctt gcc gag cgc cga tac gcg gac tcg aat acc aac acc ttt acc 672Ser Leu Ala Glu Arg Arg Tyr Ala Asp Ser Asn Thr Asn Thr Phe Thr 210 215 220 gca cag ctt ggc ctg act tcg cct gcc ctg ggg acc gtg gcg gta ttt 720Ala Gln Leu Gly Leu Thr Ser Pro Ala Leu Gly Thr Val Ala Val Phe 225 230 235 240 ggg cgt atg tcc gac agc agc tat gtc cat cgc gtc ctt ccc ggc att 768Gly Arg Met Ser Asp Ser Ser Tyr Val His Arg Val Leu Pro Gly Ile 245 250 255 acc ggc cag gac ggg atg aag agc tac gcg gcc ggc gtc cag ctc gag 816Thr Gly Gln Asp Gly Met Lys Ser Tyr Ala Ala Gly Val Gln Leu Glu 260 265 270 cgc tcg gtg gcc aac cga ctc cat ttc aac ggc tcg gtg aat tac acc 864Arg Ser Val Ala Asn Arg Leu His Phe Asn Gly Ser Val Asn Tyr Thr 275 280 285 gag gtt gac cca aag ctc gca tcc acc aaa gga ttc aag ggc gta gga 912Glu Val Asp Pro Lys Leu Ala Ser Thr Lys Gly Phe Lys Gly Val Gly 290 295 300 ttt aac gtt tcc ggc gat tat gct ggt gat cag tac agc ctc caa ttg 960Phe Asn Val Ser Gly Asp Tyr Ala Gly Asp Gln Tyr Ser Leu Gln Leu 305 310 315 320 ctg gct tca cga tcg ccc cag cct tca ctt ctt ctg ttc gtg ggt tac 1008Leu Ala Ser Arg Ser Pro Gln Pro Ser Leu Leu Leu Phe Val Gly Tyr 325 330 335 gag att gtg aca gcg gtt tcg gcg aat gcg acg cgc cgg ctg agc gat 1056Glu Ile Val Thr Ala Val Ser Ala Asn Ala Thr Arg Arg Leu Ser Asp 340 345 350 cgc att cag ata tcg ctg caa ggc agc cga acc tgg cgc gag ctc gcg 1104Arg Ile Gln Ile Ser Leu Gln Gly Ser Arg Thr Trp Arg Glu Leu Ala 355 360 365 tct tcg cgg ctg ctc acc aac gtg ccg att tcc ggc aac gac aac acc 1152Ser Ser Arg Leu Leu Thr Asn Val Pro Ile Ser Gly Asn Asp Asn Thr 370 375 380 tcg acg ttg ttc gcc tcc gct acc ttc cgg ccg aat cgc cgg ctg agc 1200Ser Thr Leu Phe Ala Ser Ala Thr Phe Arg Pro Asn Arg Arg Leu Ser 385 390 395 400 ttt gtg ctg ggt gcc ggc ctt cag cgg cgc acc agc aac acg cag cta 1248Phe Val Leu Gly Ala Gly Leu Gln Arg Arg Thr Ser Asn Thr Gln Leu 405 410 415 tac agt tac agc tcc aaa cgc atc aat ctc tcg acg tcg ctt tcg ctc 1296Tyr Ser Tyr Ser Ser Lys Arg Ile Asn Leu Ser Thr Ser Leu Ser Leu 420 425 430 tga 1299 19432PRTSphingomonas sp. ATCC53159 19Met Lys Pro Arg Pro Gly Gly Thr Phe

Met Gln Val Asn Phe Asn Arg 1 5 10 15 Gln Ala Arg Lys Leu Gly Ala Gly Asn Ala Leu Ala Arg Gly Gly Pro 20 25 30 Val Leu Ala Leu Leu Ala Thr Ala Ala Trp Thr Gln Pro Ala Leu Ala 35 40 45 Gln Arg Gln Ala Phe Glu Ser Arg Pro Ser Gly Ser Glu Arg Gln Val 50 55 60 Asp Ile Arg Ala Thr Gly Ser Leu Glu Tyr Asp Asp Asn Val Val Leu 65 70 75 80 Asn Asp Gln Arg Ile Thr Asp Gly Ala Arg Gly Asp Val Ile Ala Ser 85 90 95 Pro Gly Leu Asp Val Thr Leu Val Leu Pro Arg Ala Thr Gly Gln Leu 100 105 110 Tyr Leu Thr Gly Asn Val Gly Tyr Arg Phe Tyr Lys Arg Tyr Thr Asn 115 120 125 Phe Asn Arg Glu Gln Ile Ser Leu Thr Gly Gly Ala Asp Gln Arg Phe 130 135 140 Ala Ser Cys Val Val His Gly Glu Val Gly Tyr Gln Arg His Leu Thr 145 150 155 160 Asp Leu Ser Ser Ile Leu Ile Gln Asp Thr Thr Pro Ala Leu Asn Asn 165 170 175 Thr Glu Glu Ala Arg Gln Tyr Thr Ala Asp Ile Gly Cys Gly Ala Thr 180 185 190 Tyr Gly Leu Arg Pro Ala Val Ser Tyr Thr Arg Asn Glu Val Arg Asn 195 200 205 Ser Leu Ala Glu Arg Arg Tyr Ala Asp Ser Asn Thr Asn Thr Phe Thr 210 215 220 Ala Gln Leu Gly Leu Thr Ser Pro Ala Leu Gly Thr Val Ala Val Phe 225 230 235 240 Gly Arg Met Ser Asp Ser Ser Tyr Val His Arg Val Leu Pro Gly Ile 245 250 255 Thr Gly Gln Asp Gly Met Lys Ser Tyr Ala Ala Gly Val Gln Leu Glu 260 265 270 Arg Ser Val Ala Asn Arg Leu His Phe Asn Gly Ser Val Asn Tyr Thr 275 280 285 Glu Val Asp Pro Lys Leu Ala Ser Thr Lys Gly Phe Lys Gly Val Gly 290 295 300 Phe Asn Val Ser Gly Asp Tyr Ala Gly Asp Gln Tyr Ser Leu Gln Leu 305 310 315 320 Leu Ala Ser Arg Ser Pro Gln Pro Ser Leu Leu Leu Phe Val Gly Tyr 325 330 335 Glu Ile Val Thr Ala Val Ser Ala Asn Ala Thr Arg Arg Leu Ser Asp 340 345 350 Arg Ile Gln Ile Ser Leu Gln Gly Ser Arg Thr Trp Arg Glu Leu Ala 355 360 365 Ser Ser Arg Leu Leu Thr Asn Val Pro Ile Ser Gly Asn Asp Asn Thr 370 375 380 Ser Thr Leu Phe Ala Ser Ala Thr Phe Arg Pro Asn Arg Arg Leu Ser 385 390 395 400 Phe Val Leu Gly Ala Gly Leu Gln Arg Arg Thr Ser Asn Thr Gln Leu 405 410 415 Tyr Ser Tyr Ser Ser Lys Arg Ile Asn Leu Ser Thr Ser Leu Ser Leu 420 425 430 20918DNASphingomonas sp. ATCC53159CDS(1)..(918) 20atg cat atc aag aat cgc ttc gtg aat atc tcg acg ttg gcc atc gcc 48Met His Ile Lys Asn Arg Phe Val Asn Ile Ser Thr Leu Ala Ile Ala 1 5 10 15 gcc gcg ctg gcc acg ccg gcg gcg gcg cag atc ccc acg cgg tcc gtg 96Ala Ala Leu Ala Thr Pro Ala Ala Ala Gln Ile Pro Thr Arg Ser Val 20 25 30 ccc gcg ccg gcc cgc ccg cgg cct gca acg ccg ccg gcg caa cag cag 144Pro Ala Pro Ala Arg Pro Arg Pro Ala Thr Pro Pro Ala Gln Gln Gln 35 40 45 aac cag gcg ccg tcg acg ccc gca gcg gca acc ccg gcg cag acc gcc 192Asn Gln Ala Pro Ser Thr Pro Ala Ala Ala Thr Pro Ala Gln Thr Ala 50 55 60 gca acc gtt gcc cct gca gca acc gca ccc gca ggt tac aaa atc ggc 240Ala Thr Val Ala Pro Ala Ala Thr Ala Pro Ala Gly Tyr Lys Ile Gly 65 70 75 80 gtg gac gac gtg atc gag gcc gac gtg ctc ggc cag acc gac ttc aag 288Val Asp Asp Val Ile Glu Ala Asp Val Leu Gly Gln Thr Asp Phe Lys 85 90 95 acg cgc gcc cgt gtg cag gcg gac ggc acg gtg acc ctg ccc tat ctg 336Thr Arg Ala Arg Val Gln Ala Asp Gly Thr Val Thr Leu Pro Tyr Leu 100 105 110 ggc gcc gtg cag gtc aag ggc gag acc gcg acc tcg ctc gcc gaa aag 384Gly Ala Val Gln Val Lys Gly Glu Thr Ala Thr Ser Leu Ala Glu Lys 115 120 125 ctg gcc ggg ctg ctg cgc gcc ggc ggc tat tat gcc aag ccg atc gtc 432Leu Ala Gly Leu Leu Arg Ala Gly Gly Tyr Tyr Ala Lys Pro Ile Val 130 135 140 agc gtc gaa atc gtc ggt ttc gtc agc aac tat gtg acg gtg ctg ggc 480Ser Val Glu Ile Val Gly Phe Val Ser Asn Tyr Val Thr Val Leu Gly 145 150 155 160 cag gtg aac agt tcc ggc ctg cag ccg gtc gac cgc ggc tat cac gtt 528Gln Val Asn Ser Ser Gly Leu Gln Pro Val Asp Arg Gly Tyr His Val 165 170 175 tcc gag atc atc gcc cgt gcc ggc ggc ctg cgc ccc gaa gcg gcc gat 576Ser Glu Ile Ile Ala Arg Ala Gly Gly Leu Arg Pro Glu Ala Ala Asp 180 185 190 ttc gtc gtt ctc acc cgc gcc gat ggc tcc agc gcc aag ctg gac tac 624Phe Val Val Leu Thr Arg Ala Asp Gly Ser Ser Ala Lys Leu Asp Tyr 195 200 205 aag aag ctc gcc caa ggt ggc ccc aat gac gat ccg atg gtg acg ccc 672Lys Lys Leu Ala Gln Gly Gly Pro Asn Asp Asp Pro Met Val Thr Pro 210 215 220 ggg gac aag gtc ttt gtc ccg gaa gtc gag cat ttc tac att tat ggt 720Gly Asp Lys Val Phe Val Pro Glu Val Glu His Phe Tyr Ile Tyr Gly 225 230 235 240 caa att aac gcg cct ggc gta tac gcg att cga tcg gac atg acg ctc 768Gln Ile Asn Ala Pro Gly Val Tyr Ala Ile Arg Ser Asp Met Thr Leu 245 250 255 cgt cgc gcg ctg gcc cag ggc ggt ggg ctt gcc ccc gca ggc tcc gtc 816Arg Arg Ala Leu Ala Gln Gly Gly Gly Leu Ala Pro Ala Gly Ser Val 260 265 270 aag cgt gtg aag gtc acg cgg gat ggc aat gaa ctc aag ttg aag ctg 864Lys Arg Val Lys Val Thr Arg Asp Gly Asn Glu Leu Lys Leu Lys Leu 275 280 285 gac gat ccg att ctc cca ggc gac acg atc gtc atc ggc gaa cga ttg 912Asp Asp Pro Ile Leu Pro Gly Asp Thr Ile Val Ile Gly Glu Arg Leu 290 295 300 ttc tga 918Phe 305 21305PRTSphingomonas sp. ATCC53159 21Met His Ile Lys Asn Arg Phe Val Asn Ile Ser Thr Leu Ala Ile Ala 1 5 10 15 Ala Ala Leu Ala Thr Pro Ala Ala Ala Gln Ile Pro Thr Arg Ser Val 20 25 30 Pro Ala Pro Ala Arg Pro Arg Pro Ala Thr Pro Pro Ala Gln Gln Gln 35 40 45 Asn Gln Ala Pro Ser Thr Pro Ala Ala Ala Thr Pro Ala Gln Thr Ala 50 55 60 Ala Thr Val Ala Pro Ala Ala Thr Ala Pro Ala Gly Tyr Lys Ile Gly 65 70 75 80 Val Asp Asp Val Ile Glu Ala Asp Val Leu Gly Gln Thr Asp Phe Lys 85 90 95 Thr Arg Ala Arg Val Gln Ala Asp Gly Thr Val Thr Leu Pro Tyr Leu 100 105 110 Gly Ala Val Gln Val Lys Gly Glu Thr Ala Thr Ser Leu Ala Glu Lys 115 120 125 Leu Ala Gly Leu Leu Arg Ala Gly Gly Tyr Tyr Ala Lys Pro Ile Val 130 135 140 Ser Val Glu Ile Val Gly Phe Val Ser Asn Tyr Val Thr Val Leu Gly 145 150 155 160 Gln Val Asn Ser Ser Gly Leu Gln Pro Val Asp Arg Gly Tyr His Val 165 170 175 Ser Glu Ile Ile Ala Arg Ala Gly Gly Leu Arg Pro Glu Ala Ala Asp 180 185 190 Phe Val Val Leu Thr Arg Ala Asp Gly Ser Ser Ala Lys Leu Asp Tyr 195 200 205 Lys Lys Leu Ala Gln Gly Gly Pro Asn Asp Asp Pro Met Val Thr Pro 210 215 220 Gly Asp Lys Val Phe Val Pro Glu Val Glu His Phe Tyr Ile Tyr Gly 225 230 235 240 Gln Ile Asn Ala Pro Gly Val Tyr Ala Ile Arg Ser Asp Met Thr Leu 245 250 255 Arg Arg Ala Leu Ala Gln Gly Gly Gly Leu Ala Pro Ala Gly Ser Val 260 265 270 Lys Arg Val Lys Val Thr Arg Asp Gly Asn Glu Leu Lys Leu Lys Leu 275 280 285 Asp Asp Pro Ile Leu Pro Gly Asp Thr Ile Val Ile Gly Glu Arg Leu 290 295 300 Phe 305 221347DNASphingomonas sp. ATCC53159CDS(1)..(1347) 22gtg aat atc att cag ttc ttc cgc att ctg tgg gtg cgc cga tgg atc 48Val Asn Ile Ile Gln Phe Phe Arg Ile Leu Trp Val Arg Arg Trp Ile 1 5 10 15 atc ctc ccg gcg ttt ctc gtt tgc gtt acc act gcc acc att gtg gtc 96Ile Leu Pro Ala Phe Leu Val Cys Val Thr Thr Ala Thr Ile Val Val 20 25 30 cag ttt ctg ccc gaa cgc tac aag gcc act acg cgg gtg gtg ctc gac 144Gln Phe Leu Pro Glu Arg Tyr Lys Ala Thr Thr Arg Val Val Leu Asp 35 40 45 acg ttt aag ccc gat ccc gtc acc gga cag gtg atg agc tcg cag ttc 192Thr Phe Lys Pro Asp Pro Val Thr Gly Gln Val Met Ser Ser Gln Phe 50 55 60 atg cgc gcc tat gtc gag act cag acc cag ctg atc gag gac tat gcg 240Met Arg Ala Tyr Val Glu Thr Gln Thr Gln Leu Ile Glu Asp Tyr Ala 65 70 75 80 acc gcc ggt cgc gtg gtc gac gaa ctg ggc tgg gtg aat gat ccg gcg 288Thr Ala Gly Arg Val Val Asp Glu Leu Gly Trp Val Asn Asp Pro Ala 85 90 95 aac atc tcc gcg ttc aac aac tcg tcc gcg gct gcc acc ggc gac atc 336Asn Ile Ser Ala Phe Asn Asn Ser Ser Ala Ala Ala Thr Gly Asp Ile 100 105 110 cgc cgc tgg ctc gcc aag cag atc atc gac aat acc aag gcc gat gtg 384Arg Arg Trp Leu Ala Lys Gln Ile Ile Asp Asn Thr Lys Ala Asp Val 115 120 125 atg gag ggg agc aac atc ctc gaa atc acc tat tcg gac agc tcg ccc 432Met Glu Gly Ser Asn Ile Leu Glu Ile Thr Tyr Ser Asp Ser Ser Pro 130 135 140 gag cgc gcc gaa cgc atc gcc aac ctg atc cgc acc tcg ttc ctc gcc 480Glu Arg Ala Glu Arg Ile Ala Asn Leu Ile Arg Thr Ser Phe Leu Ala 145 150 155 160 cag tcg ctc gcc gcc aag cgc cag gcc gcg acc aag tcg gcc gac tgg 528Gln Ser Leu Ala Ala Lys Arg Gln Ala Ala Thr Lys Ser Ala Asp Trp 165 170 175 tac gcc cag cag gcc gaa gct gcc cgc gat tcg ctc gct gcg gcg gtc 576Tyr Ala Gln Gln Ala Glu Ala Ala Arg Asp Ser Leu Ala Ala Ala Val 180 185 190 cag gcc cgc acc gat ttc gtg aag aag acc ggc atc gtg ctg acc gaa 624Gln Ala Arg Thr Asp Phe Val Lys Lys Thr Gly Ile Val Leu Thr Glu 195 200 205 acc ggc gcc gac ctg gaa acc cag aag ctc cag cag atc gag ggg cag 672Thr Gly Ala Asp Leu Glu Thr Gln Lys Leu Gln Gln Ile Glu Gly Gln 210 215 220 acg acg acc gcc acc gcc ccg gtt gcc atg gcc ccc agc ggc atg ggc 720Thr Thr Thr Ala Thr Ala Pro Val Ala Met Ala Pro Ser Gly Met Gly 225 230 235 240 ccg gcg cag atg cag ctc gcc cag atc gac cag cag atc cag cag gca 768Pro Ala Gln Met Gln Leu Ala Gln Ile Asp Gln Gln Ile Gln Gln Ala 245 250 255 gcg acc agc cta ggt ccg aac cac cca act ttc cag gcc ttg cag cgg 816Ala Thr Ser Leu Gly Pro Asn His Pro Thr Phe Gln Ala Leu Gln Arg 260 265 270 cag cgc gaa gtg ttc gcc aag gca gcg gcg gcg gaa cgc gcg cag gcg 864Gln Arg Glu Val Phe Ala Lys Ala Ala Ala Ala Glu Arg Ala Gln Ala 275 280 285 aac ggc gta tcc ggt ccg gca cgc ggg gcc atc gaa agc gca gcc aac 912Asn Gly Val Ser Gly Pro Ala Arg Gly Ala Ile Glu Ser Ala Ala Asn 290 295 300 gcc cag cgc gcg cgg gtt ctc ggc aat cgt cag gat gtc gac aag ctt 960Ala Gln Arg Ala Arg Val Leu Gly Asn Arg Gln Asp Val Asp Lys Leu 305 310 315 320 acg cag ctg cag cgt gac gtc tcg ctg aag cag gat cag tac atg aag 1008Thr Gln Leu Gln Arg Asp Val Ser Leu Lys Gln Asp Gln Tyr Met Lys 325 330 335 gcg gca cag cgc gtc gcc gat ctg cgg ctg gaa gca agc agc aac gat 1056Ala Ala Gln Arg Val Ala Asp Leu Arg Leu Glu Ala Ser Ser Asn Asp 340 345 350 gtc ggc atg tcg acg ctc agc gaa gca tcg gcg ccg gaa acg ccc tat 1104Val Gly Met Ser Thr Leu Ser Glu Ala Ser Ala Pro Glu Thr Pro Tyr 355 360 365 tac ccc aag gtg ccg ctc atc atc ggt ggt gca gcc ggc ttc ggc ctc 1152Tyr Pro Lys Val Pro Leu Ile Ile Gly Gly Ala Ala Gly Phe Gly Leu 370 375 380 ggg ctc ggt ctg ctg gtc gcg ctg ctc gtc gag ctg ctc ggc cgc cgc 1200Gly Leu Gly Leu Leu Val Ala Leu Leu Val Glu Leu Leu Gly Arg Arg 385 390 395 400 gtc cgc agc ccc gag gat ctg gaa gtt gcg atc gat gca ccg gtg ctg 1248Val Arg Ser Pro Glu Asp Leu Glu Val Ala Ile Asp Ala Pro Val Leu 405 410 415 ggc gtg atc cag agc cgc gcc tcg ctt gcc gcc cgc ctt cgc cgc gcc 1296Gly Val Ile Gln Ser Arg Ala Ser Leu Ala Ala Arg Leu Arg Arg Ala 420 425 430 caa gaa acc ctc ggc gaa ggt gcc gac acg cac gga gct tca gta aac 1344Gln Glu Thr Leu Gly Glu Gly Ala Asp Thr His Gly Ala Ser Val Asn 435 440 445 tga 1347 23448PRTSphingomonas sp. ATCC53159 23Val Asn Ile Ile Gln Phe Phe Arg Ile Leu Trp Val Arg Arg Trp Ile 1 5 10 15 Ile Leu Pro Ala Phe Leu Val Cys Val Thr Thr Ala Thr Ile Val Val 20 25 30 Gln Phe Leu Pro Glu Arg Tyr Lys Ala Thr Thr Arg Val Val Leu Asp 35 40 45 Thr Phe Lys Pro Asp Pro Val Thr Gly Gln Val Met Ser Ser Gln Phe 50 55 60 Met Arg Ala Tyr Val Glu Thr Gln Thr Gln Leu Ile Glu Asp Tyr Ala 65 70 75 80 Thr Ala Gly Arg Val Val Asp Glu Leu Gly Trp Val Asn Asp Pro Ala 85 90 95 Asn Ile Ser Ala Phe Asn Asn Ser Ser Ala Ala Ala Thr Gly Asp Ile 100 105 110 Arg Arg Trp Leu Ala Lys Gln Ile Ile Asp Asn Thr Lys Ala Asp Val 115 120 125 Met Glu Gly Ser Asn Ile Leu Glu Ile Thr Tyr Ser Asp Ser Ser Pro 130 135 140 Glu Arg Ala Glu Arg Ile Ala Asn Leu Ile Arg Thr Ser Phe Leu Ala 145 150 155 160 Gln Ser Leu Ala Ala Lys Arg Gln Ala Ala Thr Lys Ser Ala Asp Trp 165 170 175 Tyr Ala Gln Gln Ala Glu Ala Ala Arg Asp Ser Leu Ala Ala Ala Val 180 185 190 Gln Ala Arg Thr Asp Phe Val Lys Lys Thr Gly Ile Val Leu Thr Glu 195 200 205 Thr Gly Ala Asp Leu Glu Thr Gln Lys Leu Gln Gln Ile Glu Gly Gln 210 215 220

Thr Thr Thr Ala Thr Ala Pro Val Ala Met Ala Pro Ser Gly Met Gly 225 230 235 240 Pro Ala Gln Met Gln Leu Ala Gln Ile Asp Gln Gln Ile Gln Gln Ala 245 250 255 Ala Thr Ser Leu Gly Pro Asn His Pro Thr Phe Gln Ala Leu Gln Arg 260 265 270 Gln Arg Glu Val Phe Ala Lys Ala Ala Ala Ala Glu Arg Ala Gln Ala 275 280 285 Asn Gly Val Ser Gly Pro Ala Arg Gly Ala Ile Glu Ser Ala Ala Asn 290 295 300 Ala Gln Arg Ala Arg Val Leu Gly Asn Arg Gln Asp Val Asp Lys Leu 305 310 315 320 Thr Gln Leu Gln Arg Asp Val Ser Leu Lys Gln Asp Gln Tyr Met Lys 325 330 335 Ala Ala Gln Arg Val Ala Asp Leu Arg Leu Glu Ala Ser Ser Asn Asp 340 345 350 Val Gly Met Ser Thr Leu Ser Glu Ala Ser Ala Pro Glu Thr Pro Tyr 355 360 365 Tyr Pro Lys Val Pro Leu Ile Ile Gly Gly Ala Ala Gly Phe Gly Leu 370 375 380 Gly Leu Gly Leu Leu Val Ala Leu Leu Val Glu Leu Leu Gly Arg Arg 385 390 395 400 Val Arg Ser Pro Glu Asp Leu Glu Val Ala Ile Asp Ala Pro Val Leu 405 410 415 Gly Val Ile Gln Ser Arg Ala Ser Leu Ala Ala Arg Leu Arg Arg Ala 420 425 430 Gln Glu Thr Leu Gly Glu Gly Ala Asp Thr His Gly Ala Ser Val Asn 435 440 445 24708DNASphingomonas sp. ATCC53159CDS(1)..(708) 24atg gac gcg atg acc agc gaa ccg ctg ccc gaa ggc gat cgt ccg agc 48Met Asp Ala Met Thr Ser Glu Pro Leu Pro Glu Gly Asp Arg Pro Ser 1 5 10 15 gcc gtg ccg acc acg ccg gat acg atc ggc atg ctc gaa tac cag ctc 96Ala Val Pro Thr Thr Pro Asp Thr Ile Gly Met Leu Glu Tyr Gln Leu 20 25 30 gtc ctc tcc gat ccg acc ggg atc gag gcg gaa gcg atc cgc gcg cta 144Val Leu Ser Asp Pro Thr Gly Ile Glu Ala Glu Ala Ile Arg Ala Leu 35 40 45 cgc acg cgc atc atg acc cag cac ctc cgc gag ggc cgg cgc gcg ctc 192Arg Thr Arg Ile Met Thr Gln His Leu Arg Glu Gly Arg Arg Ala Leu 50 55 60 gcg atc tgc gcc gcc tcg gcg gga tcc ggc tgc agc ttc acc gcc gtc 240Ala Ile Cys Ala Ala Ser Ala Gly Ser Gly Cys Ser Phe Thr Ala Val 65 70 75 80 aat ctg gcg acg gcg ctg gcg cag atc ggc gtt aag act gcg ctg gtc 288Asn Leu Ala Thr Ala Leu Ala Gln Ile Gly Val Lys Thr Ala Leu Val 85 90 95 gat gcc aat ctg cgc gat ccc agc atc ggc gca gcc ttc ggc ctc gcc 336Asp Ala Asn Leu Arg Asp Pro Ser Ile Gly Ala Ala Phe Gly Leu Ala 100 105 110 gcc gac aag ccc ggc ctg gcc gat tat ctc gcc tcg ggc gat gtc gac 384Ala Asp Lys Pro Gly Leu Ala Asp Tyr Leu Ala Ser Gly Asp Val Asp 115 120 125 ctc gcc tcg atc atc cat gcg acc cgc ctc gac cag ctc tcg atc atc 432Leu Ala Ser Ile Ile His Ala Thr Arg Leu Asp Gln Leu Ser Ile Ile 130 135 140 ccg gcc ggg cat gtc gag cac agc ccg cag gaa ctg ctc gcg tcc gaa 480Pro Ala Gly His Val Glu His Ser Pro Gln Glu Leu Leu Ala Ser Glu 145 150 155 160 cag ttc cat gat ctg gcg acg cag ctg ctg cgc gag ttc gac atc acg 528Gln Phe His Asp Leu Ala Thr Gln Leu Leu Arg Glu Phe Asp Ile Thr 165 170 175 atc ttc gac acc acg gcg tcc aac acc tgc gcc gac gcg cag cgt gtc 576Ile Phe Asp Thr Thr Ala Ser Asn Thr Cys Ala Asp Ala Gln Arg Val 180 185 190 gcg cat atc gcc ggc tat gcg atc atc gtg gcg cgc aag gat gcg agc 624Ala His Ile Ala Gly Tyr Ala Ile Ile Val Ala Arg Lys Asp Ala Ser 195 200 205 tac atc cgc gac gtg aac acg ctc agc cgc acg ctg cgt gca gac cgc 672Tyr Ile Arg Asp Val Asn Thr Leu Ser Arg Thr Leu Arg Ala Asp Arg 210 215 220 acc aac gtc atc ggc tgc gta ctg aac ggc tat tga 708Thr Asn Val Ile Gly Cys Val Leu Asn Gly Tyr 225 230 235 25235PRTSphingomonas sp. ATCC53159 25Met Asp Ala Met Thr Ser Glu Pro Leu Pro Glu Gly Asp Arg Pro Ser 1 5 10 15 Ala Val Pro Thr Thr Pro Asp Thr Ile Gly Met Leu Glu Tyr Gln Leu 20 25 30 Val Leu Ser Asp Pro Thr Gly Ile Glu Ala Glu Ala Ile Arg Ala Leu 35 40 45 Arg Thr Arg Ile Met Thr Gln His Leu Arg Glu Gly Arg Arg Ala Leu 50 55 60 Ala Ile Cys Ala Ala Ser Ala Gly Ser Gly Cys Ser Phe Thr Ala Val 65 70 75 80 Asn Leu Ala Thr Ala Leu Ala Gln Ile Gly Val Lys Thr Ala Leu Val 85 90 95 Asp Ala Asn Leu Arg Asp Pro Ser Ile Gly Ala Ala Phe Gly Leu Ala 100 105 110 Ala Asp Lys Pro Gly Leu Ala Asp Tyr Leu Ala Ser Gly Asp Val Asp 115 120 125 Leu Ala Ser Ile Ile His Ala Thr Arg Leu Asp Gln Leu Ser Ile Ile 130 135 140 Pro Ala Gly His Val Glu His Ser Pro Gln Glu Leu Leu Ala Ser Glu 145 150 155 160 Gln Phe His Asp Leu Ala Thr Gln Leu Leu Arg Glu Phe Asp Ile Thr 165 170 175 Ile Phe Asp Thr Thr Ala Ser Asn Thr Cys Ala Asp Ala Gln Arg Val 180 185 190 Ala His Ile Ala Gly Tyr Ala Ile Ile Val Ala Arg Lys Asp Ala Ser 195 200 205 Tyr Ile Arg Asp Val Asn Thr Leu Ser Arg Thr Leu Arg Ala Asp Arg 210 215 220 Thr Asn Val Ile Gly Cys Val Leu Asn Gly Tyr 225 230 235 26882DNASphingomonas sp. ATCC53159CDS(1)..(882) 26atg gca gcg acc gcg atg acg cgg cag cag gag agg aag ggc ggt ggc 48Met Ala Ala Thr Ala Met Thr Arg Gln Gln Glu Arg Lys Gly Gly Gly 1 5 10 15 tat tgg ctg gcc gtt gcc ggt ctt gcc gcg cta acc atc ccg acc ttc 96Tyr Trp Leu Ala Val Ala Gly Leu Ala Ala Leu Thr Ile Pro Thr Phe 20 25 30 atc acc ctg ggt cgc gag gtt tgg agt gcg gaa ggc ggc gtg cag ggt 144Ile Thr Leu Gly Arg Glu Val Trp Ser Ala Glu Gly Gly Val Gln Gly 35 40 45 ccg atc gtg ctc gcc acg ggc gcc tgg atg ctg gcc cgc cag tgc tcg 192Pro Ile Val Leu Ala Thr Gly Ala Trp Met Leu Ala Arg Gln Cys Ser 50 55 60 acg atc gag gcg cta cgc cgc ccc ggc agc gtg ctg ctc ggc gcg ctg 240Thr Ile Glu Ala Leu Arg Arg Pro Gly Ser Val Leu Leu Gly Ala Leu 65 70 75 80 ttc ctg ctg gcg acg ctt gcc ttc tac acc gtt gga cgg gtg ttc gac 288Phe Leu Leu Ala Thr Leu Ala Phe Tyr Thr Val Gly Arg Val Phe Asp 85 90 95 ttc atc agt gtc gaa acc ttc gga ctg gtc gcg acc tat ctg gtc gtc 336Phe Ile Ser Val Glu Thr Phe Gly Leu Val Ala Thr Tyr Leu Val Val 100 105 110 gcc tat ctc tat ttc ggt gcc agg gtg ctc cgt gcc gcc tgg ttc ccg 384Ala Tyr Leu Tyr Phe Gly Ala Arg Val Leu Arg Ala Ala Trp Phe Pro 115 120 125 gtg ctg tgg ctg ttc ttc ctg gtg ccg ccg ccc ggc tgg gcc gtc gac 432Val Leu Trp Leu Phe Phe Leu Val Pro Pro Pro Gly Trp Ala Val Asp 130 135 140 cgc atc acc gca ccg ctc aag gag ttc gtc tcc tat gcg gca acg ggc 480Arg Ile Thr Ala Pro Leu Lys Glu Phe Val Ser Tyr Ala Ala Thr Gly 145 150 155 160 ctg ctt tcc tgg gtg gat tat ccg atc ctg cgc cag ggc gtg aca ctg 528Leu Leu Ser Trp Val Asp Tyr Pro Ile Leu Arg Gln Gly Val Thr Leu 165 170 175 ttc gtc ggc ccc tat cag ctg ctc gtc gaa gat gcc tgt tcg ggt ctg 576Phe Val Gly Pro Tyr Gln Leu Leu Val Glu Asp Ala Cys Ser Gly Leu 180 185 190 cgc tcg ctg tcc agc ctg gtc gtc gtg acg ctg ctc tac atc tac atc 624Arg Ser Leu Ser Ser Leu Val Val Val Thr Leu Leu Tyr Ile Tyr Ile 195 200 205 aag aac aag ccg tcc tgg cgc tac gcg gcg ttc atc gca gcg ctg gtg 672Lys Asn Lys Pro Ser Trp Arg Tyr Ala Ala Phe Ile Ala Ala Leu Val 210 215 220 atc ccg gtg gca gtg gtg acc aac gtc ctg cgg atc atc atc ctg gta 720Ile Pro Val Ala Val Val Thr Asn Val Leu Arg Ile Ile Ile Leu Val 225 230 235 240 ctg atc acc tat cat ctg ggc gac gag gcg gcg cag agc ttc ctc cac 768Leu Ile Thr Tyr His Leu Gly Asp Glu Ala Ala Gln Ser Phe Leu His 245 250 255 gtc tcc acc ggc atg gtg atg ttc gtg gtc gcc ctg ctt tgc atc ttc 816Val Ser Thr Gly Met Val Met Phe Val Val Ala Leu Leu Cys Ile Phe 260 265 270 gcg atc gac tgg gtg gtc gag caa ctt ctt ctc ctg cgt cgg agg cat 864Ala Ile Asp Trp Val Val Glu Gln Leu Leu Leu Leu Arg Arg Arg His 275 280 285 cat gtt caa ccg gcg tga 882His Val Gln Pro Ala 290 27293PRTSphingomonas sp. ATCC53159 27Met Ala Ala Thr Ala Met Thr Arg Gln Gln Glu Arg Lys Gly Gly Gly 1 5 10 15 Tyr Trp Leu Ala Val Ala Gly Leu Ala Ala Leu Thr Ile Pro Thr Phe 20 25 30 Ile Thr Leu Gly Arg Glu Val Trp Ser Ala Glu Gly Gly Val Gln Gly 35 40 45 Pro Ile Val Leu Ala Thr Gly Ala Trp Met Leu Ala Arg Gln Cys Ser 50 55 60 Thr Ile Glu Ala Leu Arg Arg Pro Gly Ser Val Leu Leu Gly Ala Leu 65 70 75 80 Phe Leu Leu Ala Thr Leu Ala Phe Tyr Thr Val Gly Arg Val Phe Asp 85 90 95 Phe Ile Ser Val Glu Thr Phe Gly Leu Val Ala Thr Tyr Leu Val Val 100 105 110 Ala Tyr Leu Tyr Phe Gly Ala Arg Val Leu Arg Ala Ala Trp Phe Pro 115 120 125 Val Leu Trp Leu Phe Phe Leu Val Pro Pro Pro Gly Trp Ala Val Asp 130 135 140 Arg Ile Thr Ala Pro Leu Lys Glu Phe Val Ser Tyr Ala Ala Thr Gly 145 150 155 160 Leu Leu Ser Trp Val Asp Tyr Pro Ile Leu Arg Gln Gly Val Thr Leu 165 170 175 Phe Val Gly Pro Tyr Gln Leu Leu Val Glu Asp Ala Cys Ser Gly Leu 180 185 190 Arg Ser Leu Ser Ser Leu Val Val Val Thr Leu Leu Tyr Ile Tyr Ile 195 200 205 Lys Asn Lys Pro Ser Trp Arg Tyr Ala Ala Phe Ile Ala Ala Leu Val 210 215 220 Ile Pro Val Ala Val Val Thr Asn Val Leu Arg Ile Ile Ile Leu Val 225 230 235 240 Leu Ile Thr Tyr His Leu Gly Asp Glu Ala Ala Gln Ser Phe Leu His 245 250 255 Val Ser Thr Gly Met Val Met Phe Val Val Ala Leu Leu Cys Ile Phe 260 265 270 Ala Ile Asp Trp Val Val Glu Gln Leu Leu Leu Leu Arg Arg Arg His 275 280 285 His Val Gln Pro Ala 290 28699DNASphingomonas sp. ATCC53159CDS(1)..(699) 28atg ttc aac cgg cgt gac ctg ctg atc ggc gca ggc tgc ttc gcc gcc 48Met Phe Asn Arg Arg Asp Leu Leu Ile Gly Ala Gly Cys Phe Ala Ala 1 5 10 15 gct ggc gcc tcg ctc ggc ctg aag ccg cac cgg cgg atg gac ctg ctg 96Ala Gly Ala Ser Leu Gly Leu Lys Pro His Arg Arg Met Asp Leu Leu 20 25 30 ggc ggc acc aag ctc gac acg ctg atg ccc aag gca ttc ggc gca tgg 144Gly Gly Thr Lys Leu Asp Thr Leu Met Pro Lys Ala Phe Gly Ala Trp 35 40 45 aag gca gag gat acc ggt tcg ctg atc gcg ccg gcg cgc gaa ggc agc 192Lys Ala Glu Asp Thr Gly Ser Leu Ile Ala Pro Ala Arg Glu Gly Ser 50 55 60 ctg gag gac aag ctc tac aac cag gtg gtc acc cgc gcc ttc tcc cgc 240Leu Glu Asp Lys Leu Tyr Asn Gln Val Val Thr Arg Ala Phe Ser Arg 65 70 75 80 gcg gac ggt gcc caa gtg atg ctg ctg atc gcc tat ggc aac gcc cag 288Ala Asp Gly Ala Gln Val Met Leu Leu Ile Ala Tyr Gly Asn Ala Gln 85 90 95 acc gat cta ctg cag ctg cac cgg ccg gaa ata tgc tac ccg ttc ttc 336Thr Asp Leu Leu Gln Leu His Arg Pro Glu Ile Cys Tyr Pro Phe Phe 100 105 110 ggc ttc acc gtg gtg gaa agc cat gag cag acc atc ccg gtg acg ccg 384Gly Phe Thr Val Val Glu Ser His Glu Gln Thr Ile Pro Val Thr Pro 115 120 125 cag gtg acg atc ccc ggt cgc gcg ctg acc gcc acc aac ttc aac cgc 432Gln Val Thr Ile Pro Gly Arg Ala Leu Thr Ala Thr Asn Phe Asn Arg 130 135 140 acc gag cag atc ctc tac tgg acc cgc gtc ggc gaa tat ctg ccg cag 480Thr Glu Gln Ile Leu Tyr Trp Thr Arg Val Gly Glu Tyr Leu Pro Gln 145 150 155 160 aac ggc aat cag cag atg ctc gcg cgg ctg aag agc cag gtc cag ggc 528Asn Gly Asn Gln Gln Met Leu Ala Arg Leu Lys Ser Gln Val Gln Gly 165 170 175 tgg atc gtc gac ggt gtg ctg gtg cgc atc tcg acg gtg acg ccc gag 576Trp Ile Val Asp Gly Val Leu Val Arg Ile Ser Thr Val Thr Pro Glu 180 185 190 gcg gaa gat ggc ctg agc gcc aat ctc gat ttc gcg cgc gag ctg gtg 624Ala Glu Asp Gly Leu Ser Ala Asn Leu Asp Phe Ala Arg Glu Leu Val 195 200 205 aag acg ctc gac ccg cgc gtg ctg cgc ccg ctg ctc ggg aac ggg ctc 672Lys Thr Leu Asp Pro Arg Val Leu Arg Pro Leu Leu Gly Asn Gly Leu 210 215 220 aca cgg cag ctc ggt cac cag gtc tga 699Thr Arg Gln Leu Gly His Gln Val 225 230 29232PRTSphingomonas sp. ATCC53159 29Met Phe Asn Arg Arg Asp Leu Leu Ile Gly Ala Gly Cys Phe Ala Ala 1 5 10 15 Ala Gly Ala Ser Leu Gly Leu Lys Pro His Arg Arg Met Asp Leu Leu 20 25 30 Gly Gly Thr Lys Leu Asp Thr Leu Met Pro Lys Ala Phe Gly Ala Trp 35 40 45 Lys Ala Glu Asp Thr Gly Ser Leu Ile Ala Pro Ala Arg Glu Gly Ser 50 55 60 Leu Glu Asp Lys Leu Tyr Asn Gln Val Val Thr Arg Ala Phe Ser Arg 65 70 75 80 Ala Asp Gly Ala Gln Val Met Leu Leu Ile Ala Tyr Gly Asn Ala Gln 85 90 95 Thr Asp Leu Leu Gln Leu His Arg Pro Glu Ile Cys Tyr Pro Phe Phe 100 105 110 Gly Phe Thr Val Val Glu Ser His Glu Gln Thr Ile Pro Val Thr Pro 115 120 125 Gln Val Thr Ile Pro Gly Arg Ala Leu Thr Ala Thr Asn Phe Asn Arg 130 135 140 Thr Glu Gln Ile Leu Tyr Trp Thr Arg Val Gly Glu Tyr Leu Pro Gln 145 150 155 160 Asn Gly Asn Gln Gln Met Leu Ala Arg Leu Lys Ser Gln Val Gln Gly 165 170 175 Trp Ile Val Asp

Gly Val Leu Val Arg Ile Ser Thr Val Thr Pro Glu 180 185 190 Ala Glu Asp Gly Leu Ser Ala Asn Leu Asp Phe Ala Arg Glu Leu Val 195 200 205 Lys Thr Leu Asp Pro Arg Val Leu Arg Pro Leu Leu Gly Asn Gly Leu 210 215 220 Thr Arg Gln Leu Gly His Gln Val 225 230 301395DNASphingomonas sp. ATCC53159CDS(1)..(1395) 30atg aac gcc gtt gtt ccg atg cgc cgc ggc ggc ccg ctc gcc cgc atg 48Met Asn Ala Val Val Pro Met Arg Arg Gly Gly Pro Leu Ala Arg Met 1 5 10 15 cgc gat acc gtg ctg cct gcc cgc gtc gac gct tat gac acc gcc ttc 96Arg Asp Thr Val Leu Pro Ala Arg Val Asp Ala Tyr Asp Thr Ala Phe 20 25 30 ctg cct gcc gcg ctg gag atc atc gag cgg ccg gtt tcg ccc acc gcg 144Leu Pro Ala Ala Leu Glu Ile Ile Glu Arg Pro Val Ser Pro Thr Ala 35 40 45 cgg ctt acc gcc aag gtg atg ctg gcc ggg ctg gcg atc acc gcc gcc 192Arg Leu Thr Ala Lys Val Met Leu Ala Gly Leu Ala Ile Thr Ala Ala 50 55 60 tgg ctg gcg atc ggc aag gtc gaa gtc gtc gcg ccg acg cag ggg cgg 240Trp Leu Ala Ile Gly Lys Val Glu Val Val Ala Pro Thr Gln Gly Arg 65 70 75 80 atc gcg ccg atc ggc gag acc aag atc gtc cag tcg ccc gaa tcg ggg 288Ile Ala Pro Ile Gly Glu Thr Lys Ile Val Gln Ser Pro Glu Ser Gly 85 90 95 atc gtc cgc cgc atc ctg gtg ggc gag ggg cag aag gtc gcc aag ggc 336Ile Val Arg Arg Ile Leu Val Gly Glu Gly Gln Lys Val Ala Lys Gly 100 105 110 cag gtg ctg atc acg ctc gac ccg acc gtg tcg tcg gcg gag gcg gca 384Gln Val Leu Ile Thr Leu Asp Pro Thr Val Ser Ser Ala Glu Ala Ala 115 120 125 cag gcg aag gtg gcg ctg ctc agc gcc cag ctc gac gcc gca cgc aac 432Gln Ala Lys Val Ala Leu Leu Ser Ala Gln Leu Asp Ala Ala Arg Asn 130 135 140 cag gcg atc atc gac gcg ctg gac ggc agg ggc ttc cgc ttc gtc gcg 480Gln Ala Ile Ile Asp Ala Leu Asp Gly Arg Gly Phe Arg Phe Val Ala 145 150 155 160 cct gcc gcc gcc agc ccg ggc gaa gtg gcg acg cat cgc ggc ctc gcc 528Pro Ala Ala Ala Ser Pro Gly Glu Val Ala Thr His Arg Gly Leu Ala 165 170 175 cgc gcc cgg ctg ggc cag atc gag gcg gcg ctg gcc ggc ggc cgc tcc 576Arg Ala Arg Leu Gly Gln Ile Glu Ala Ala Leu Ala Gly Gly Arg Ser 180 185 190 gat cgc ggt gcc gcc gtc tcg gcc gcg gcc gag gcg cag gca cag gtg 624Asp Arg Gly Ala Ala Val Ser Ala Ala Ala Glu Ala Gln Ala Gln Val 195 200 205 cgg aag ctc gaa cag tcg ctg ccg ctg ctc gaa cag cag atc gcc gcg 672Arg Lys Leu Glu Gln Ser Leu Pro Leu Leu Glu Gln Gln Ile Ala Ala 210 215 220 aac gag acg atg gcc gcc aag ggc tat gtc tcg aag ctg cgc gtc gtg 720Asn Glu Thr Met Ala Ala Lys Gly Tyr Val Ser Lys Leu Arg Val Val 225 230 235 240 gag atg cgt cgc cag ctg atc gcc gag cgg cag gac ctg acg gcg gcg 768Glu Met Arg Arg Gln Leu Ile Ala Glu Arg Gln Asp Leu Thr Ala Ala 245 250 255 cgc gct acg ctc gcc aaa ctc ggc cag cag tcg ctg agc gtc tcc agc 816Arg Ala Thr Leu Ala Lys Leu Gly Gln Gln Ser Leu Ser Val Ser Ser 260 265 270 ctg tcg gcc aag acg cgc gag gag gcg cgg gcg cag gtg ctg cag gat 864Leu Ser Ala Lys Thr Arg Glu Glu Ala Arg Ala Gln Val Leu Gln Asp 275 280 285 ctg gtc aag gcg cag gac gag gtg cgt gcc cgc ggc gag gac gtc gcc 912Leu Val Lys Ala Gln Asp Glu Val Arg Ala Arg Gly Glu Asp Val Ala 290 295 300 aag gcg aat ctg cgc agc tcg ttc cgc gaa ctg cgc gcg ccg gtg agc 960Lys Ala Asn Leu Arg Ser Ser Phe Arg Glu Leu Arg Ala Pro Val Ser 305 310 315 320 ggt acc gtc tcg cag ctg cag gtc cac acc gaa ggc ggc gtg gtg gaa 1008Gly Thr Val Ser Gln Leu Gln Val His Thr Glu Gly Gly Val Val Glu 325 330 335 ggg gcc aag ccg ctc ctc agc ctg gtt ccc gac aat gcc cgg ctc gag 1056Gly Ala Lys Pro Leu Leu Ser Leu Val Pro Asp Asn Ala Arg Leu Glu 340 345 350 gcc gag gtg atg gtc gac aac agc gac atc ggc ttc gtc cac atc ggc 1104Ala Glu Val Met Val Asp Asn Ser Asp Ile Gly Phe Val His Ile Gly 355 360 365 atg ccg gta aag gtg aag ctg cag gcc ttt ccc tat acc cgc tac ggc 1152Met Pro Val Lys Val Lys Leu Gln Ala Phe Pro Tyr Thr Arg Tyr Gly 370 375 380 atg att ccc ggc acg gtg gcg ggc atc agc ccc gag gcg gtg cag atg 1200Met Ile Pro Gly Thr Val Ala Gly Ile Ser Pro Glu Ala Val Gln Met 385 390 395 400 aag gag aac cag ccg ccg gtc tac aag gcg cgg atc gcg ctg gcg cgc 1248Lys Glu Asn Gln Pro Pro Val Tyr Lys Ala Arg Ile Ala Leu Ala Arg 405 410 415 ggg tat gtg ctg gcc cat ggc gca cag gtg ccg ctg cgg ccg ggg atg 1296Gly Tyr Val Leu Ala His Gly Ala Gln Val Pro Leu Arg Pro Gly Met 420 425 430 ctc gcg agc gcg gac atc gtc acc ggc aag cga acc ctg ttc agc tat 1344Leu Ala Ser Ala Asp Ile Val Thr Gly Lys Arg Thr Leu Phe Ser Tyr 435 440 445 ctg gtg ggg ccc gtg ctc gag acg ggg agt gac gcg ctg cac gag cgg 1392Leu Val Gly Pro Val Leu Glu Thr Gly Ser Asp Ala Leu His Glu Arg 450 455 460 tga 139531464PRTSphingomonas sp. ATCC53159 31Met Asn Ala Val Val Pro Met Arg Arg Gly Gly Pro Leu Ala Arg Met 1 5 10 15 Arg Asp Thr Val Leu Pro Ala Arg Val Asp Ala Tyr Asp Thr Ala Phe 20 25 30 Leu Pro Ala Ala Leu Glu Ile Ile Glu Arg Pro Val Ser Pro Thr Ala 35 40 45 Arg Leu Thr Ala Lys Val Met Leu Ala Gly Leu Ala Ile Thr Ala Ala 50 55 60 Trp Leu Ala Ile Gly Lys Val Glu Val Val Ala Pro Thr Gln Gly Arg 65 70 75 80 Ile Ala Pro Ile Gly Glu Thr Lys Ile Val Gln Ser Pro Glu Ser Gly 85 90 95 Ile Val Arg Arg Ile Leu Val Gly Glu Gly Gln Lys Val Ala Lys Gly 100 105 110 Gln Val Leu Ile Thr Leu Asp Pro Thr Val Ser Ser Ala Glu Ala Ala 115 120 125 Gln Ala Lys Val Ala Leu Leu Ser Ala Gln Leu Asp Ala Ala Arg Asn 130 135 140 Gln Ala Ile Ile Asp Ala Leu Asp Gly Arg Gly Phe Arg Phe Val Ala 145 150 155 160 Pro Ala Ala Ala Ser Pro Gly Glu Val Ala Thr His Arg Gly Leu Ala 165 170 175 Arg Ala Arg Leu Gly Gln Ile Glu Ala Ala Leu Ala Gly Gly Arg Ser 180 185 190 Asp Arg Gly Ala Ala Val Ser Ala Ala Ala Glu Ala Gln Ala Gln Val 195 200 205 Arg Lys Leu Glu Gln Ser Leu Pro Leu Leu Glu Gln Gln Ile Ala Ala 210 215 220 Asn Glu Thr Met Ala Ala Lys Gly Tyr Val Ser Lys Leu Arg Val Val 225 230 235 240 Glu Met Arg Arg Gln Leu Ile Ala Glu Arg Gln Asp Leu Thr Ala Ala 245 250 255 Arg Ala Thr Leu Ala Lys Leu Gly Gln Gln Ser Leu Ser Val Ser Ser 260 265 270 Leu Ser Ala Lys Thr Arg Glu Glu Ala Arg Ala Gln Val Leu Gln Asp 275 280 285 Leu Val Lys Ala Gln Asp Glu Val Arg Ala Arg Gly Glu Asp Val Ala 290 295 300 Lys Ala Asn Leu Arg Ser Ser Phe Arg Glu Leu Arg Ala Pro Val Ser 305 310 315 320 Gly Thr Val Ser Gln Leu Gln Val His Thr Glu Gly Gly Val Val Glu 325 330 335 Gly Ala Lys Pro Leu Leu Ser Leu Val Pro Asp Asn Ala Arg Leu Glu 340 345 350 Ala Glu Val Met Val Asp Asn Ser Asp Ile Gly Phe Val His Ile Gly 355 360 365 Met Pro Val Lys Val Lys Leu Gln Ala Phe Pro Tyr Thr Arg Tyr Gly 370 375 380 Met Ile Pro Gly Thr Val Ala Gly Ile Ser Pro Glu Ala Val Gln Met 385 390 395 400 Lys Glu Asn Gln Pro Pro Val Tyr Lys Ala Arg Ile Ala Leu Ala Arg 405 410 415 Gly Tyr Val Leu Ala His Gly Ala Gln Val Pro Leu Arg Pro Gly Met 420 425 430 Leu Ala Ser Ala Asp Ile Val Thr Gly Lys Arg Thr Leu Phe Ser Tyr 435 440 445 Leu Val Gly Pro Val Leu Glu Thr Gly Ser Asp Ala Leu His Glu Arg 450 455 460 322187DNASphingomonas sp. ATCC53159CDS(1)..(2187) 32atg aca cgc gac gaa atg cag gcc acc ctg cag agc gcg ctc gcg gcc 48Met Thr Arg Asp Glu Met Gln Ala Thr Leu Gln Ser Ala Leu Ala Ala 1 5 10 15 cat ggg gcg gcg gag cgc gag gcg gag ctg cgc gaa tcc gga ctg gtg 96His Gly Ala Ala Glu Arg Glu Ala Glu Leu Arg Glu Ser Gly Leu Val 20 25 30 gcg ttg tcg ctg ctg ctc ggc gcg cac aac atc gcc atc acg ccc gaa 144Ala Leu Ser Leu Leu Leu Gly Ala His Asn Ile Ala Ile Thr Pro Glu 35 40 45 cag ctg cgc cac gcg ctg ggc cat gcc gag gcg gca agc gcc gac gac 192Gln Leu Arg His Ala Leu Gly His Ala Glu Ala Ala Ser Ala Asp Asp 50 55 60 ctg atc ctc ctg gcc aag cgc cag cag ggc gtg cgc gcc aag gcc gtc 240Leu Ile Leu Leu Ala Lys Arg Gln Gln Gly Val Arg Ala Lys Ala Val 65 70 75 80 gag gtg ccg cgc ggc gga ctc gcc cgc cag ccg ctg ccc gcg atc gcc 288Glu Val Pro Arg Gly Gly Leu Ala Arg Gln Pro Leu Pro Ala Ile Ala 85 90 95 gac ggg ccc gaa ggc tgg ttc gtg atc ggc ggc ctg acc gaa cat ggc 336Asp Gly Pro Glu Gly Trp Phe Val Ile Gly Gly Leu Thr Glu His Gly 100 105 110 gtg atc atc cag cgc ccg ggc cat gcc ccg gaa cag gtc gac cgg gac 384Val Ile Ile Gln Arg Pro Gly His Ala Pro Glu Gln Val Asp Arg Asp 115 120 125 gcg ctg gac gcg atc tgg tcc ggc gcg ctg gtg ctg ctc acc acc cgc 432Ala Leu Asp Ala Ile Trp Ser Gly Ala Leu Val Leu Leu Thr Thr Arg 130 135 140 gcg gtg gcg gga cgg ccg ctg cgg ttc ggc ctc tcc tgg ttc acc gcg 480Ala Val Ala Gly Arg Pro Leu Arg Phe Gly Leu Ser Trp Phe Thr Ala 145 150 155 160 cag ttc cgg cgc tat cgc acg ctg ttc ctc gag gtg ctc ggc atc acc 528Gln Phe Arg Arg Tyr Arg Thr Leu Phe Leu Glu Val Leu Gly Ile Thr 165 170 175 ctc gcg ctc aac ctg ctc ggc ctc gcc gcg ccg ctg ttg ttc cag agc 576Leu Ala Leu Asn Leu Leu Gly Leu Ala Ala Pro Leu Leu Phe Gln Ser 180 185 190 gtg atc gac aag gtg ctg atc cac aac agc atg agc acg ctg agc gtg 624Val Ile Asp Lys Val Leu Ile His Asn Ser Met Ser Thr Leu Ser Val 195 200 205 ctc gcc ttc gcc ttc ctg gcg gtt tcg gtg tgg gaa gtg gcg ctc ggc 672Leu Ala Phe Ala Phe Leu Ala Val Ser Val Trp Glu Val Ala Leu Gly 210 215 220 tgg atc cgc acc cgc ctg ttc acc gag acg acg cag aag atc gac gtc 720Trp Ile Arg Thr Arg Leu Phe Thr Glu Thr Thr Gln Lys Ile Asp Val 225 230 235 240 gag ctg ggt gcc cgg ctg ttc cac cac ctg ctg gcg ctg ccg ctc gcc 768Glu Leu Gly Ala Arg Leu Phe His His Leu Leu Ala Leu Pro Leu Ala 245 250 255 tat ttc gag aag cgc cgc gtg ggc gac acc gtc acc cgc gtc cgc cag 816Tyr Phe Glu Lys Arg Arg Val Gly Asp Thr Val Thr Arg Val Arg Gln 260 265 270 ctc gag acg atc cgc gaa ttc ctt acc agc gcc tcg ctg acg gtg atg 864Leu Glu Thr Ile Arg Glu Phe Leu Thr Ser Ala Ser Leu Thr Val Met 275 280 285 gtg gac ccg ctg ttc acc ttc gtg ttc ctc gcc gcg atg ctg ttc tac 912Val Asp Pro Leu Phe Thr Phe Val Phe Leu Ala Ala Met Leu Phe Tyr 290 295 300 tcg ccg atg ctc tcg ggc atc gtg ctc gtg tcg ctg atc gcc tat gcg 960Ser Pro Met Leu Ser Gly Ile Val Leu Val Ser Leu Ile Ala Tyr Ala 305 310 315 320 atc gta tcg ttc agc gtc gcc ggg ccg ctc cgc gcg cgg gtg gag gac 1008Ile Val Ser Phe Ser Val Ala Gly Pro Leu Arg Ala Arg Val Glu Asp 325 330 335 aag ttc gag aag agc tcc gcc agc aac gcg ctg ctc gtc gag agc gtc 1056Lys Phe Glu Lys Ser Ser Ala Ser Asn Ala Leu Leu Val Glu Ser Val 340 345 350 tcg ggc atc cac acg atc aag gcg acc gcg gtc gag ccg cac tgg cag 1104Ser Gly Ile His Thr Ile Lys Ala Thr Ala Val Glu Pro His Trp Gln 355 360 365 aat cgc tgg gag cgc cag ctc gcc gcc cat acc gcc gcg tcg cag cgg 1152Asn Arg Trp Glu Arg Gln Leu Ala Ala His Thr Ala Ala Ser Gln Arg 370 375 380 ctg atc aat acc gcc aac acc ggc agc cag gcg atc gag ctg atc tcg 1200Leu Ile Asn Thr Ala Asn Thr Gly Ser Gln Ala Ile Glu Leu Ile Ser 385 390 395 400 aag ctg agc ttc gcg gcg atc ctg ttc ttc ggc gcc aag gcg gtg atc 1248Lys Leu Ser Phe Ala Ala Ile Leu Phe Phe Gly Ala Lys Ala Val Ile 405 410 415 ggc ggc gcg atg agc gta ggc gcg ctg gtg gcg ttc aac atg ttc gcc 1296Gly Gly Ala Met Ser Val Gly Ala Leu Val Ala Phe Asn Met Phe Ala 420 425 430 cag cgc gtg tcc ggg ccg gtg atc cgc atg gcg cag ctg tgg cag gat 1344Gln Arg Val Ser Gly Pro Val Ile Arg Met Ala Gln Leu Trp Gln Asp 435 440 445 ttc cag cag gtg cgc atc tcg gtc gag cgg ctg ggc gac gtg ctc aac 1392Phe Gln Gln Val Arg Ile Ser Val Glu Arg Leu Gly Asp Val Leu Asn 450 455 460 cat ccg gtg gaa ccg cgc ccg gcc tcg gcg gcg acg ctg ccg gtg ctg 1440His Pro Val Glu Pro Arg Pro Ala Ser Ala Ala Thr Leu Pro Val Leu 465 470 475 480 cgc ggt gcg att cgc ttc gag aat gtc agc ttc cgc tat gcc gag gac 1488Arg Gly Ala Ile Arg Phe Glu Asn Val Ser Phe Arg Tyr Ala Glu Asp 485 490 495 cag ccg ccg gtg ctg agc gac atc acg ctc gac att ccg gcg ggc acc 1536Gln Pro Pro Val Leu Ser Asp Ile Thr Leu Asp Ile Pro Ala Gly Thr 500 505 510 tcg ctc ggc atc gtc ggt tcg tcg ggc tcg ggc aag tcg acg ctg gcc 1584Ser Leu Gly Ile Val Gly Ser Ser Gly Ser Gly Lys Ser Thr Leu Ala 515 520 525 aag ctg ctc cag cgg ctc aac ctg ccg aat ctc ggc cgc gtg ctg gtc 1632Lys Leu Leu Gln Arg Leu Asn Leu Pro Asn Leu Gly Arg Val Leu Val 530 535 540 gac gag gtc gac gtg gcg cag ctc gat ccc gcc tgg ctg cgt cgc cag 1680Asp Glu Val Asp Val Ala Gln Leu Asp Pro Ala Trp Leu Arg Arg Gln 545 550 555 560 atc ggc gtc gtg ctg cag gag aat ctg ctg ttc agc cgc tcg atc cgc

1728Ile Gly Val Val Leu Gln Glu Asn Leu Leu Phe Ser Arg Ser Ile Arg 565 570 575 gag aac atc gcg ctc tcc aac ccc gcc atg ccg ttc gag aat gtc gtc 1776Glu Asn Ile Ala Leu Ser Asn Pro Ala Met Pro Phe Glu Asn Val Val 580 585 590 gcg gcg gcg acg ctg gcc ggc gcg cat gat ttc atc ctg cgc cag ccg 1824Ala Ala Ala Thr Leu Ala Gly Ala His Asp Phe Ile Leu Arg Gln Pro 595 600 605 cgc ggc tat gac acc gag atc gtc gag cgc ggc gtc aat ctc tcc ggc 1872Arg Gly Tyr Asp Thr Glu Ile Val Glu Arg Gly Val Asn Leu Ser Gly 610 615 620 ggc cag cgc cag cgg ctc gcc atc gcc cgc gcg ctc gtc ggc aat ccg 1920Gly Gln Arg Gln Arg Leu Ala Ile Ala Arg Ala Leu Val Gly Asn Pro 625 630 635 640 cgc atc ctg gtg ttc gac gaa gcg acc tcg gcg ctc gat gcc gag agc 1968Arg Ile Leu Val Phe Asp Glu Ala Thr Ser Ala Leu Asp Ala Glu Ser 645 650 655 gag gag ctg atc cag aac aac ctg cgc gcc atc tcg gcc ggc cgc acg 2016Glu Glu Leu Ile Gln Asn Asn Leu Arg Ala Ile Ser Ala Gly Arg Thr 660 665 670 ctc gtg gtg atc gcg cat cgc ctg agc gcg gtg cgc agc tgc gac cgg 2064Leu Val Val Ile Ala His Arg Leu Ser Ala Val Arg Ser Cys Asp Arg 675 680 685 atc atc acg ctc gaa cag ggc cgc atc gtc gag agc ggc cga cac gac 2112Ile Ile Thr Leu Glu Gln Gly Arg Ile Val Glu Ser Gly Arg His Asp 690 695 700 gaa ttg ttg cgc ctg ggc ggc cgc tat gcc gac ctg cac cgc cgc cag 2160Glu Leu Leu Arg Leu Gly Gly Arg Tyr Ala Asp Leu His Arg Arg Gln 705 710 715 720 ggc ggc tat ggg gag att gcc gca tga 2187Gly Gly Tyr Gly Glu Ile Ala Ala 725 33728PRTSphingomonas sp. ATCC53159 33Met Thr Arg Asp Glu Met Gln Ala Thr Leu Gln Ser Ala Leu Ala Ala 1 5 10 15 His Gly Ala Ala Glu Arg Glu Ala Glu Leu Arg Glu Ser Gly Leu Val 20 25 30 Ala Leu Ser Leu Leu Leu Gly Ala His Asn Ile Ala Ile Thr Pro Glu 35 40 45 Gln Leu Arg His Ala Leu Gly His Ala Glu Ala Ala Ser Ala Asp Asp 50 55 60 Leu Ile Leu Leu Ala Lys Arg Gln Gln Gly Val Arg Ala Lys Ala Val 65 70 75 80 Glu Val Pro Arg Gly Gly Leu Ala Arg Gln Pro Leu Pro Ala Ile Ala 85 90 95 Asp Gly Pro Glu Gly Trp Phe Val Ile Gly Gly Leu Thr Glu His Gly 100 105 110 Val Ile Ile Gln Arg Pro Gly His Ala Pro Glu Gln Val Asp Arg Asp 115 120 125 Ala Leu Asp Ala Ile Trp Ser Gly Ala Leu Val Leu Leu Thr Thr Arg 130 135 140 Ala Val Ala Gly Arg Pro Leu Arg Phe Gly Leu Ser Trp Phe Thr Ala 145 150 155 160 Gln Phe Arg Arg Tyr Arg Thr Leu Phe Leu Glu Val Leu Gly Ile Thr 165 170 175 Leu Ala Leu Asn Leu Leu Gly Leu Ala Ala Pro Leu Leu Phe Gln Ser 180 185 190 Val Ile Asp Lys Val Leu Ile His Asn Ser Met Ser Thr Leu Ser Val 195 200 205 Leu Ala Phe Ala Phe Leu Ala Val Ser Val Trp Glu Val Ala Leu Gly 210 215 220 Trp Ile Arg Thr Arg Leu Phe Thr Glu Thr Thr Gln Lys Ile Asp Val 225 230 235 240 Glu Leu Gly Ala Arg Leu Phe His His Leu Leu Ala Leu Pro Leu Ala 245 250 255 Tyr Phe Glu Lys Arg Arg Val Gly Asp Thr Val Thr Arg Val Arg Gln 260 265 270 Leu Glu Thr Ile Arg Glu Phe Leu Thr Ser Ala Ser Leu Thr Val Met 275 280 285 Val Asp Pro Leu Phe Thr Phe Val Phe Leu Ala Ala Met Leu Phe Tyr 290 295 300 Ser Pro Met Leu Ser Gly Ile Val Leu Val Ser Leu Ile Ala Tyr Ala 305 310 315 320 Ile Val Ser Phe Ser Val Ala Gly Pro Leu Arg Ala Arg Val Glu Asp 325 330 335 Lys Phe Glu Lys Ser Ser Ala Ser Asn Ala Leu Leu Val Glu Ser Val 340 345 350 Ser Gly Ile His Thr Ile Lys Ala Thr Ala Val Glu Pro His Trp Gln 355 360 365 Asn Arg Trp Glu Arg Gln Leu Ala Ala His Thr Ala Ala Ser Gln Arg 370 375 380 Leu Ile Asn Thr Ala Asn Thr Gly Ser Gln Ala Ile Glu Leu Ile Ser 385 390 395 400 Lys Leu Ser Phe Ala Ala Ile Leu Phe Phe Gly Ala Lys Ala Val Ile 405 410 415 Gly Gly Ala Met Ser Val Gly Ala Leu Val Ala Phe Asn Met Phe Ala 420 425 430 Gln Arg Val Ser Gly Pro Val Ile Arg Met Ala Gln Leu Trp Gln Asp 435 440 445 Phe Gln Gln Val Arg Ile Ser Val Glu Arg Leu Gly Asp Val Leu Asn 450 455 460 His Pro Val Glu Pro Arg Pro Ala Ser Ala Ala Thr Leu Pro Val Leu 465 470 475 480 Arg Gly Ala Ile Arg Phe Glu Asn Val Ser Phe Arg Tyr Ala Glu Asp 485 490 495 Gln Pro Pro Val Leu Ser Asp Ile Thr Leu Asp Ile Pro Ala Gly Thr 500 505 510 Ser Leu Gly Ile Val Gly Ser Ser Gly Ser Gly Lys Ser Thr Leu Ala 515 520 525 Lys Leu Leu Gln Arg Leu Asn Leu Pro Asn Leu Gly Arg Val Leu Val 530 535 540 Asp Glu Val Asp Val Ala Gln Leu Asp Pro Ala Trp Leu Arg Arg Gln 545 550 555 560 Ile Gly Val Val Leu Gln Glu Asn Leu Leu Phe Ser Arg Ser Ile Arg 565 570 575 Glu Asn Ile Ala Leu Ser Asn Pro Ala Met Pro Phe Glu Asn Val Val 580 585 590 Ala Ala Ala Thr Leu Ala Gly Ala His Asp Phe Ile Leu Arg Gln Pro 595 600 605 Arg Gly Tyr Asp Thr Glu Ile Val Glu Arg Gly Val Asn Leu Ser Gly 610 615 620 Gly Gln Arg Gln Arg Leu Ala Ile Ala Arg Ala Leu Val Gly Asn Pro 625 630 635 640 Arg Ile Leu Val Phe Asp Glu Ala Thr Ser Ala Leu Asp Ala Glu Ser 645 650 655 Glu Glu Leu Ile Gln Asn Asn Leu Arg Ala Ile Ser Ala Gly Arg Thr 660 665 670 Leu Val Val Ile Ala His Arg Leu Ser Ala Val Arg Ser Cys Asp Arg 675 680 685 Ile Ile Thr Leu Glu Gln Gly Arg Ile Val Glu Ser Gly Arg His Asp 690 695 700 Glu Leu Leu Arg Leu Gly Gly Arg Tyr Ala Asp Leu His Arg Arg Gln 705 710 715 720 Gly Gly Tyr Gly Glu Ile Ala Ala 725 341413DNASphingomonas sp. ATCC53159CDS(1)..(1413) 34atg aac gct ttc gaa gca cag cgc gcc ttt gag gag caa ctt cgg gcg 48Met Asn Ala Phe Glu Ala Gln Arg Ala Phe Glu Glu Gln Leu Arg Ala 1 5 10 15 cat tcc cgg gtt acg cca tct gcc gct ccc gtg tgg cgt cgc tcg acg 96His Ser Arg Val Thr Pro Ser Ala Ala Pro Val Trp Arg Arg Ser Thr 20 25 30 ctg cgg atg gtc ctc tat acc gag ttg ctg ctg ctg gac agt ctc tcg 144Leu Arg Met Val Leu Tyr Thr Glu Leu Leu Leu Leu Asp Ser Leu Ser 35 40 45 atc ctg gcc gga ttc cac gtc gcg gcg ggc acg cgc gac ggc aac tgg 192Ile Leu Ala Gly Phe His Val Ala Ala Gly Thr Arg Asp Gly Asn Trp 50 55 60 ctg tcg ctg gcg ggc atc aac gtc ggc gtc ttc ctg ctg ccg atc gct 240Leu Ser Leu Ala Gly Ile Asn Val Gly Val Phe Leu Leu Pro Ile Ala 65 70 75 80 ctc ggc acc gcg ctc gca agc ggc acc tac tcg ctg aac tgc ctg cgc 288Leu Gly Thr Ala Leu Ala Ser Gly Thr Tyr Ser Leu Asn Cys Leu Arg 85 90 95 tac ccg gtc agc ggc gtg aag agc atc ttc tcg gca ttc ttc ttc tcg 336Tyr Pro Val Ser Gly Val Lys Ser Ile Phe Ser Ala Phe Phe Phe Ser 100 105 110 atc ttc gtc gtc ctg ctc ggc agc tac ctg ctg acg gcc gag ctg ccg 384Ile Phe Val Val Leu Leu Gly Ser Tyr Leu Leu Thr Ala Glu Leu Pro 115 120 125 ctg tcc cgc gtg cag ctg gcg gag ggc gcg atc ctc tcg ctg gtc ctc 432Leu Ser Arg Val Gln Leu Ala Glu Gly Ala Ile Leu Ser Leu Val Leu 130 135 140 ctg atg gtg ggc cgc ctg atg ttc cgc cgc cac gtc cgc gcg gtt acc 480Leu Met Val Gly Arg Leu Met Phe Arg Arg His Val Arg Ala Val Thr 145 150 155 160 ggc ggc agg ctg ctc gac gaa ctg gtc atc atc gac ggc gtc tcg ctc 528Gly Gly Arg Leu Leu Asp Glu Leu Val Ile Ile Asp Gly Val Ser Leu 165 170 175 gac gtc gcg ggc aat gcg gtc gcg ctc gac gcg cgg atc atc aat ctc 576Asp Val Ala Gly Asn Ala Val Ala Leu Asp Ala Arg Ile Ile Asn Leu 180 185 190 tcg ccg aac ccg cgc gat ccg caa atg ctg cat cgc ctg ggc acc acc 624Ser Pro Asn Pro Arg Asp Pro Gln Met Leu His Arg Leu Gly Thr Thr 195 200 205 gtg atc ggg ttc gac cgg gtg atc gtc gcc tgc acc aag gag cat cgc 672Val Ile Gly Phe Asp Arg Val Ile Val Ala Cys Thr Lys Glu His Arg 210 215 220 gcg gtc tgg gcg ctg ctg ctc aag ggc atg aac atc aag ggc gag atc 720Ala Val Trp Ala Leu Leu Leu Lys Gly Met Asn Ile Lys Gly Glu Ile 225 230 235 240 ctc gtc ccc cag ttc aat gcg ctg ggc gcg atc ggc gtg gac gcc ttt 768Leu Val Pro Gln Phe Asn Ala Leu Gly Ala Ile Gly Val Asp Ala Phe 245 250 255 gac ggg aag gat acg ctg gtc gtc tcg cag ggc ccg ctc aac atg ccc 816Asp Gly Lys Asp Thr Leu Val Val Ser Gln Gly Pro Leu Asn Met Pro 260 265 270 aac cgc gcg aag aag cgc gcg ctc gat ctc gcg atc acc gta ccg gcc 864Asn Arg Ala Lys Lys Arg Ala Leu Asp Leu Ala Ile Thr Val Pro Ala 275 280 285 gtg ctc gcg ctg gcg ccg ctg atg atc ctg gtg gcg atc ctg atc aag 912Val Leu Ala Leu Ala Pro Leu Met Ile Leu Val Ala Ile Leu Ile Lys 290 295 300 ctg gag agc ccg ggc ccg gtg ttg ttc gcg cag gat cgc gtc ggc cgc 960Leu Glu Ser Pro Gly Pro Val Leu Phe Ala Gln Asp Arg Val Gly Arg 305 310 315 320 ggc aac cgg ctg ttc aag atc atg aag ttc cgc tcg atg cgc gta acg 1008Gly Asn Arg Leu Phe Lys Ile Met Lys Phe Arg Ser Met Arg Val Thr 325 330 335 ctg tgc gac gcg aac ggc aac gtc tcg gcc agc cgc gac gac gat cgc 1056Leu Cys Asp Ala Asn Gly Asn Val Ser Ala Ser Arg Asp Asp Asp Arg 340 345 350 atc acc aag gtc ggc cgc ttc atc cgc aag acc agc atc gac gaa ctg 1104Ile Thr Lys Val Gly Arg Phe Ile Arg Lys Thr Ser Ile Asp Glu Leu 355 360 365 ccg cag ctg ctg aac gtg ctg cgc ggc gac atg agc gtc gtc ggc ccg 1152Pro Gln Leu Leu Asn Val Leu Arg Gly Asp Met Ser Val Val Gly Pro 370 375 380 cgg ccg cat gcg ctg ggc tcg cgc gcc gcc gat cac ctg ttc tgg gaa 1200Arg Pro His Ala Leu Gly Ser Arg Ala Ala Asp His Leu Phe Trp Glu 385 390 395 400 atc gac gag cgc tac tgg cac cgc cac acg ctc aag ccg ggc atg acc 1248Ile Asp Glu Arg Tyr Trp His Arg His Thr Leu Lys Pro Gly Met Thr 405 410 415 ggt ctg gcc cag gtg cgc ggt ttc cgc ggg gcg acc gat cgc cgc gtc 1296Gly Leu Ala Gln Val Arg Gly Phe Arg Gly Ala Thr Asp Arg Arg Val 420 425 430 gat ctg acc aac cgg ctc cag gca gac atg gaa tat atc gac gga tgg 1344Asp Leu Thr Asn Arg Leu Gln Ala Asp Met Glu Tyr Ile Asp Gly Trp 435 440 445 gat atc tgg cgc gat atc acg atc ctg ttc aag acg ctg cgg gtg atc 1392Asp Ile Trp Arg Asp Ile Thr Ile Leu Phe Lys Thr Leu Arg Val Ile 450 455 460 gtg cat tcg aac gca ttc tga 1413Val His Ser Asn Ala Phe 465 470 35470PRTSphingomonas sp. ATCC53159 35Met Asn Ala Phe Glu Ala Gln Arg Ala Phe Glu Glu Gln Leu Arg Ala 1 5 10 15 His Ser Arg Val Thr Pro Ser Ala Ala Pro Val Trp Arg Arg Ser Thr 20 25 30 Leu Arg Met Val Leu Tyr Thr Glu Leu Leu Leu Leu Asp Ser Leu Ser 35 40 45 Ile Leu Ala Gly Phe His Val Ala Ala Gly Thr Arg Asp Gly Asn Trp 50 55 60 Leu Ser Leu Ala Gly Ile Asn Val Gly Val Phe Leu Leu Pro Ile Ala 65 70 75 80 Leu Gly Thr Ala Leu Ala Ser Gly Thr Tyr Ser Leu Asn Cys Leu Arg 85 90 95 Tyr Pro Val Ser Gly Val Lys Ser Ile Phe Ser Ala Phe Phe Phe Ser 100 105 110 Ile Phe Val Val Leu Leu Gly Ser Tyr Leu Leu Thr Ala Glu Leu Pro 115 120 125 Leu Ser Arg Val Gln Leu Ala Glu Gly Ala Ile Leu Ser Leu Val Leu 130 135 140 Leu Met Val Gly Arg Leu Met Phe Arg Arg His Val Arg Ala Val Thr 145 150 155 160 Gly Gly Arg Leu Leu Asp Glu Leu Val Ile Ile Asp Gly Val Ser Leu 165 170 175 Asp Val Ala Gly Asn Ala Val Ala Leu Asp Ala Arg Ile Ile Asn Leu 180 185 190 Ser Pro Asn Pro Arg Asp Pro Gln Met Leu His Arg Leu Gly Thr Thr 195 200 205 Val Ile Gly Phe Asp Arg Val Ile Val Ala Cys Thr Lys Glu His Arg 210 215 220 Ala Val Trp Ala Leu Leu Leu Lys Gly Met Asn Ile Lys Gly Glu Ile 225 230 235 240 Leu Val Pro Gln Phe Asn Ala Leu Gly Ala Ile Gly Val Asp Ala Phe 245 250 255 Asp Gly Lys Asp Thr Leu Val Val Ser Gln Gly Pro Leu Asn Met Pro 260 265 270 Asn Arg Ala Lys Lys Arg Ala Leu Asp Leu Ala Ile Thr Val Pro Ala 275 280 285 Val Leu Ala Leu Ala Pro Leu Met Ile Leu Val Ala Ile Leu Ile Lys 290 295 300 Leu Glu Ser Pro Gly Pro Val Leu Phe Ala Gln Asp Arg Val Gly Arg 305 310 315 320 Gly Asn Arg Leu Phe Lys Ile Met Lys Phe Arg Ser Met Arg Val Thr 325 330 335 Leu Cys Asp Ala Asn Gly Asn Val Ser Ala Ser Arg Asp Asp Asp Arg 340 345 350 Ile Thr Lys Val Gly Arg Phe Ile Arg Lys Thr Ser Ile Asp Glu Leu 355 360 365 Pro Gln Leu Leu Asn Val Leu Arg Gly Asp Met Ser Val Val Gly Pro 370 375 380 Arg Pro His Ala Leu Gly Ser Arg Ala Ala Asp His Leu Phe Trp Glu 385 390 395 400 Ile Asp Glu Arg Tyr Trp His Arg His Thr Leu Lys Pro Gly Met Thr 405 410 415 Gly Leu Ala Gln Val Arg Gly Phe Arg Gly Ala Thr Asp Arg Arg Val 420 425 430 Asp Leu Thr Asn Arg Leu Gln Ala Asp Met Glu Tyr Ile Asp Gly Trp 435 440

445 Asp Ile Trp Arg Asp Ile Thr Ile Leu Phe Lys Thr Leu Arg Val Ile 450 455 460 Val His Ser Asn Ala Phe 465 470 36879DNASphingomonas sp. ATCC53159CDS(1)..(879) 36atg aag ggc atc atc ctt gcg ggg ggc agc ggg acg cgc ctg tac ccc 48Met Lys Gly Ile Ile Leu Ala Gly Gly Ser Gly Thr Arg Leu Tyr Pro 1 5 10 15 gca acg cta tcg atc tcg aag cag ctg ctt ccc gtc tat gac aag ccg 96Ala Thr Leu Ser Ile Ser Lys Gln Leu Leu Pro Val Tyr Asp Lys Pro 20 25 30 atg atc ttc tat ccg ctg tcg gtg ctg atg ctc acc ggc atc cgg gac 144Met Ile Phe Tyr Pro Leu Ser Val Leu Met Leu Thr Gly Ile Arg Asp 35 40 45 atc ctg att atc tcc acc ccg cgc gac ctg ccg atg ttc cag gcg ctg 192Ile Leu Ile Ile Ser Thr Pro Arg Asp Leu Pro Met Phe Gln Ala Leu 50 55 60 ctg ggc gac ggc tcg gcc ttc ggc atc aac ctc agc tat gcc gag cag 240Leu Gly Asp Gly Ser Ala Phe Gly Ile Asn Leu Ser Tyr Ala Glu Gln 65 70 75 80 ccc tcc ccc aac ggg ctg gcc gaa gcg ttc atc atc ggc gcg gat ttc 288Pro Ser Pro Asn Gly Leu Ala Glu Ala Phe Ile Ile Gly Ala Asp Phe 85 90 95 gtc ggc aac gat ccc agc gcg ctg atc ctg ggc gac aac atc tat cac 336Val Gly Asn Asp Pro Ser Ala Leu Ile Leu Gly Asp Asn Ile Tyr His 100 105 110 ggc gaa aag atg ggc gag cgc tgc cag gca gcc gca gcg cag gca gcg 384Gly Glu Lys Met Gly Glu Arg Cys Gln Ala Ala Ala Ala Gln Ala Ala 115 120 125 cag ggc ggt gca aac gtc ttc gcc tat cat gtc gac gac ccc gag cgc 432Gln Gly Gly Ala Asn Val Phe Ala Tyr His Val Asp Asp Pro Glu Arg 130 135 140 tac ggc gtg gtc gcg ttc gac ccg gag acg ggc gtc gcc acc agc gtc 480Tyr Gly Val Val Ala Phe Asp Pro Glu Thr Gly Val Ala Thr Ser Val 145 150 155 160 gag gaa aag ccg gcc gag ccc aag tcc aac tgg gcg atc acc ggc ctg 528Glu Glu Lys Pro Ala Glu Pro Lys Ser Asn Trp Ala Ile Thr Gly Leu 165 170 175 tat ttc tac gac aag gac gtg gtc gac atc gcc aag tcg atc cag ccc 576Tyr Phe Tyr Asp Lys Asp Val Val Asp Ile Ala Lys Ser Ile Gln Pro 180 185 190 tcg gcg cgc ggc gaa ctc gag atc acc gac gtc aac cgc gtt tac atg 624Ser Ala Arg Gly Glu Leu Glu Ile Thr Asp Val Asn Arg Val Tyr Met 195 200 205 gag cgc ggc gac ctg cac atc acg cgc ctc ggc cgc ggc tat gcc tgg 672Glu Arg Gly Asp Leu His Ile Thr Arg Leu Gly Arg Gly Tyr Ala Trp 210 215 220 ctc gac acc ggc acg cat gac agc ctg cac gaa gcc ggc tcg ttc gtt 720Leu Asp Thr Gly Thr His Asp Ser Leu His Glu Ala Gly Ser Phe Val 225 230 235 240 cgc acg ctc gag cat cgg acg ggc gtg aag atc gcc tgc ccg gag gaa 768Arg Thr Leu Glu His Arg Thr Gly Val Lys Ile Ala Cys Pro Glu Glu 245 250 255 atc gcc ttc gaa agc ggc tgg ctc ggc gcc gaa gac ctg ctc aag cgc 816Ile Ala Phe Glu Ser Gly Trp Leu Gly Ala Glu Asp Leu Leu Lys Arg 260 265 270 gcc gcc ggc ctc ggc aag acc ggc tat gcc gcc tat ctc cgc aag gtt 864Ala Ala Gly Leu Gly Lys Thr Gly Tyr Ala Ala Tyr Leu Arg Lys Val 275 280 285 gcg acc gca gca tga 879Ala Thr Ala Ala 290 37292PRTSphingomonas sp. ATCC53159 37Met Lys Gly Ile Ile Leu Ala Gly Gly Ser Gly Thr Arg Leu Tyr Pro 1 5 10 15 Ala Thr Leu Ser Ile Ser Lys Gln Leu Leu Pro Val Tyr Asp Lys Pro 20 25 30 Met Ile Phe Tyr Pro Leu Ser Val Leu Met Leu Thr Gly Ile Arg Asp 35 40 45 Ile Leu Ile Ile Ser Thr Pro Arg Asp Leu Pro Met Phe Gln Ala Leu 50 55 60 Leu Gly Asp Gly Ser Ala Phe Gly Ile Asn Leu Ser Tyr Ala Glu Gln 65 70 75 80 Pro Ser Pro Asn Gly Leu Ala Glu Ala Phe Ile Ile Gly Ala Asp Phe 85 90 95 Val Gly Asn Asp Pro Ser Ala Leu Ile Leu Gly Asp Asn Ile Tyr His 100 105 110 Gly Glu Lys Met Gly Glu Arg Cys Gln Ala Ala Ala Ala Gln Ala Ala 115 120 125 Gln Gly Gly Ala Asn Val Phe Ala Tyr His Val Asp Asp Pro Glu Arg 130 135 140 Tyr Gly Val Val Ala Phe Asp Pro Glu Thr Gly Val Ala Thr Ser Val 145 150 155 160 Glu Glu Lys Pro Ala Glu Pro Lys Ser Asn Trp Ala Ile Thr Gly Leu 165 170 175 Tyr Phe Tyr Asp Lys Asp Val Val Asp Ile Ala Lys Ser Ile Gln Pro 180 185 190 Ser Ala Arg Gly Glu Leu Glu Ile Thr Asp Val Asn Arg Val Tyr Met 195 200 205 Glu Arg Gly Asp Leu His Ile Thr Arg Leu Gly Arg Gly Tyr Ala Trp 210 215 220 Leu Asp Thr Gly Thr His Asp Ser Leu His Glu Ala Gly Ser Phe Val 225 230 235 240 Arg Thr Leu Glu His Arg Thr Gly Val Lys Ile Ala Cys Pro Glu Glu 245 250 255 Ile Ala Phe Glu Ser Gly Trp Leu Gly Ala Glu Asp Leu Leu Lys Arg 260 265 270 Ala Ala Gly Leu Gly Lys Thr Gly Tyr Ala Ala Tyr Leu Arg Lys Val 275 280 285 Ala Thr Ala Ala 290 38567DNASphingomonas sp. ATCC53159CDS(1)..(567) 38atg acc cag gtc cat cat cac gaa ctg tcc ggc gtc atc gag ttc acg 48Met Thr Gln Val His His His Glu Leu Ser Gly Val Ile Glu Phe Thr 1 5 10 15 ccg ccc aaa tat ggc gac cac cgc ggc ttc ttc tcc gaa gtg ttc aag 96Pro Pro Lys Tyr Gly Asp His Arg Gly Phe Phe Ser Glu Val Phe Lys 20 25 30 cag tcg gtg ctc gat gcc gaa ggc gtc gag gca cgc tgg gtg cag gac 144Gln Ser Val Leu Asp Ala Glu Gly Val Glu Ala Arg Trp Val Gln Asp 35 40 45 aat cag agc ttc tcg gcg gcc ccg ggc acg atc cgc ggc ctg cat ctc 192Asn Gln Ser Phe Ser Ala Ala Pro Gly Thr Ile Arg Gly Leu His Leu 50 55 60 cag gcg ccg ccc ttc gcc cag gcc aag ctg gtc cgc gtg ttg cgc ggc 240Gln Ala Pro Pro Phe Ala Gln Ala Lys Leu Val Arg Val Leu Arg Gly 65 70 75 80 gcg atc ttc gac gtc gcg gtc gac atc cgt cgc ggc tcg ccc acc tat 288Ala Ile Phe Asp Val Ala Val Asp Ile Arg Arg Gly Ser Pro Thr Tyr 85 90 95 ggc aaa tgg gtc ggc gtc gag ctc tcg gcc gag aag tgg aac cag ctg 336Gly Lys Trp Val Gly Val Glu Leu Ser Ala Glu Lys Trp Asn Gln Leu 100 105 110 ctg gtc ccc gcc ggc tat gcg cac ggc ttc atg acg ctc gtt ccg gat 384Leu Val Pro Ala Gly Tyr Ala His Gly Phe Met Thr Leu Val Pro Asp 115 120 125 tgc gag atc ctc tac aag gtc agc gcc aaa tat tcg aag gat tcg gag 432Cys Glu Ile Leu Tyr Lys Val Ser Ala Lys Tyr Ser Lys Asp Ser Glu 130 135 140 atg gcg atc cgt tgg gac gat ccc gat ctc gcc atc gcc tgg ccg gac 480Met Ala Ile Arg Trp Asp Asp Pro Asp Leu Ala Ile Ala Trp Pro Asp 145 150 155 160 atc ggc gtc gag ccg gtc ctc tcc gaa aag gac gcg gtc gcc acg ccc 528Ile Gly Val Glu Pro Val Leu Ser Glu Lys Asp Ala Val Ala Thr Pro 165 170 175 ttc gcc gaa ttc aac acc ccc ttc ttc tat cag ggc tga 567Phe Ala Glu Phe Asn Thr Pro Phe Phe Tyr Gln Gly 180 185 39188PRTSphingomonas sp. ATCC53159 39Met Thr Gln Val His His His Glu Leu Ser Gly Val Ile Glu Phe Thr 1 5 10 15 Pro Pro Lys Tyr Gly Asp His Arg Gly Phe Phe Ser Glu Val Phe Lys 20 25 30 Gln Ser Val Leu Asp Ala Glu Gly Val Glu Ala Arg Trp Val Gln Asp 35 40 45 Asn Gln Ser Phe Ser Ala Ala Pro Gly Thr Ile Arg Gly Leu His Leu 50 55 60 Gln Ala Pro Pro Phe Ala Gln Ala Lys Leu Val Arg Val Leu Arg Gly 65 70 75 80 Ala Ile Phe Asp Val Ala Val Asp Ile Arg Arg Gly Ser Pro Thr Tyr 85 90 95 Gly Lys Trp Val Gly Val Glu Leu Ser Ala Glu Lys Trp Asn Gln Leu 100 105 110 Leu Val Pro Ala Gly Tyr Ala His Gly Phe Met Thr Leu Val Pro Asp 115 120 125 Cys Glu Ile Leu Tyr Lys Val Ser Ala Lys Tyr Ser Lys Asp Ser Glu 130 135 140 Met Ala Ile Arg Trp Asp Asp Pro Asp Leu Ala Ile Ala Trp Pro Asp 145 150 155 160 Ile Gly Val Glu Pro Val Leu Ser Glu Lys Asp Ala Val Ala Thr Pro 165 170 175 Phe Ala Glu Phe Asn Thr Pro Phe Phe Tyr Gln Gly 180 185 401062DNASphingomonas sp. ATCC53159CDS(1)..(1062) 40atg cag cag acc ttc ctc gtc acc ggc ggc gcc ggc ttc atc ggc tcg 48Met Gln Gln Thr Phe Leu Val Thr Gly Gly Ala Gly Phe Ile Gly Ser 1 5 10 15 gcg gtg gtg cgc cac ctc gtc cgc cag ggc gcg cgc gtc atc aat ctc 96Ala Val Val Arg His Leu Val Arg Gln Gly Ala Arg Val Ile Asn Leu 20 25 30 gac aag ctc acc tat gcc ggc aac ccg gcc tcg ctg act gcg atc gag 144Asp Lys Leu Thr Tyr Ala Gly Asn Pro Ala Ser Leu Thr Ala Ile Glu 35 40 45 aac gcg ccc aac tat cgc ttc gtc cat gcc gac atc gcc gac acc gcg 192Asn Ala Pro Asn Tyr Arg Phe Val His Ala Asp Ile Ala Asp Thr Ala 50 55 60 acg atc cta ccg ctg ctg cgc gag gag cag gtc gat gtg gtg atg cac 240Thr Ile Leu Pro Leu Leu Arg Glu Glu Gln Val Asp Val Val Met His 65 70 75 80 ctc gcc gcc gag agc cat gtc gat cgc tcg atc gac ggc cct ggc gag 288Leu Ala Ala Glu Ser His Val Asp Arg Ser Ile Asp Gly Pro Gly Glu 85 90 95 ttc atc gag acc aat gtc gtc ggc acc ttc aag ctg ctc cag tcg gcg 336Phe Ile Glu Thr Asn Val Val Gly Thr Phe Lys Leu Leu Gln Ser Ala 100 105 110 ctg caa tat tgg cgc gag ctg gag ggc gag aaa cgc gac gcg ttc cgc 384Leu Gln Tyr Trp Arg Glu Leu Glu Gly Glu Lys Arg Asp Ala Phe Arg 115 120 125 ttc cac cac atc tcc acc gac gaa gtg ttc ggc gac ctg ccg ttc gac 432Phe His His Ile Ser Thr Asp Glu Val Phe Gly Asp Leu Pro Phe Asp 130 135 140 agc ggc atc ttc acc gaa gag acg ccc tat gat ccc tcc tcg ccc tat 480Ser Gly Ile Phe Thr Glu Glu Thr Pro Tyr Asp Pro Ser Ser Pro Tyr 145 150 155 160 tcg gcg tcg aag gcg gcg agc gac cat ctg gtg cgc gcc tgg ggc cac 528Ser Ala Ser Lys Ala Ala Ser Asp His Leu Val Arg Ala Trp Gly His 165 170 175 acc tat ggc ctg ccg gtg gtg ctg tcg aac tgc tcg aac aat tac ggg 576Thr Tyr Gly Leu Pro Val Val Leu Ser Asn Cys Ser Asn Asn Tyr Gly 180 185 190 ccg ttc cac ttc ccc gag aag ctg atc ccg ttg acc atc ctc aac gcg 624Pro Phe His Phe Pro Glu Lys Leu Ile Pro Leu Thr Ile Leu Asn Ala 195 200 205 ctc gag ggc aag ccg ctg ccg gtc tac ggc aag ggc gag aat atc cgc 672Leu Glu Gly Lys Pro Leu Pro Val Tyr Gly Lys Gly Glu Asn Ile Arg 210 215 220 gac tgg ctg tat gtc gac gat cac gcc aag gcg ctg gcg acc atc gcc 720Asp Trp Leu Tyr Val Asp Asp His Ala Lys Ala Leu Ala Thr Ile Ala 225 230 235 240 acc acc ggc aag gtc ggc cag agc tac aat gtc ggc ggc cgc aac gag 768Thr Thr Gly Lys Val Gly Gln Ser Tyr Asn Val Gly Gly Arg Asn Glu 245 250 255 cgg acc aac ctg cag gtg gtc gag acg atc tgc gac ctg ctc gac cag 816Arg Thr Asn Leu Gln Val Val Glu Thr Ile Cys Asp Leu Leu Asp Gln 260 265 270 cgc att ccg ctg gcc gac ggt cgc aag cgc cgc gaa ctg atc acc ttc 864Arg Ile Pro Leu Ala Asp Gly Arg Lys Arg Arg Glu Leu Ile Thr Phe 275 280 285 gtc acc gat cgc ccc ggc cat gac cgc cgc tac gcg atc gac gcg acc 912Val Thr Asp Arg Pro Gly His Asp Arg Arg Tyr Ala Ile Asp Ala Thr 290 295 300 aag ctc gag acc gag ctg ggc tgg aag gct gag gag aat ttc gac acc 960Lys Leu Glu Thr Glu Leu Gly Trp Lys Ala Glu Glu Asn Phe Asp Thr 305 310 315 320 ggc atc gcc gcg acg atc gac tgg tat ctg gcg aac gag tgg tgg tgg 1008Gly Ile Ala Ala Thr Ile Asp Trp Tyr Leu Ala Asn Glu Trp Trp Trp 325 330 335 ggc ccg atc cgc tcc ggc aaa tat gcc ggc gag cgg ctg ggg cag acc 1056Gly Pro Ile Arg Ser Gly Lys Tyr Ala Gly Glu Arg Leu Gly Gln Thr 340 345 350 gcc tga 1062Ala 41353PRTSphingomonas sp. ATCC53159 41Met Gln Gln Thr Phe Leu Val Thr Gly Gly Ala Gly Phe Ile Gly Ser 1 5 10 15 Ala Val Val Arg His Leu Val Arg Gln Gly Ala Arg Val Ile Asn Leu 20 25 30 Asp Lys Leu Thr Tyr Ala Gly Asn Pro Ala Ser Leu Thr Ala Ile Glu 35 40 45 Asn Ala Pro Asn Tyr Arg Phe Val His Ala Asp Ile Ala Asp Thr Ala 50 55 60 Thr Ile Leu Pro Leu Leu Arg Glu Glu Gln Val Asp Val Val Met His 65 70 75 80 Leu Ala Ala Glu Ser His Val Asp Arg Ser Ile Asp Gly Pro Gly Glu 85 90 95 Phe Ile Glu Thr Asn Val Val Gly Thr Phe Lys Leu Leu Gln Ser Ala 100 105 110 Leu Gln Tyr Trp Arg Glu Leu Glu Gly Glu Lys Arg Asp Ala Phe Arg 115 120 125 Phe His His Ile Ser Thr Asp Glu Val Phe Gly Asp Leu Pro Phe Asp 130 135 140 Ser Gly Ile Phe Thr Glu Glu Thr Pro Tyr Asp Pro Ser Ser Pro Tyr 145 150 155 160 Ser Ala Ser Lys Ala Ala Ser Asp His Leu Val Arg Ala Trp Gly His 165 170 175 Thr Tyr Gly Leu Pro Val Val Leu Ser Asn Cys Ser Asn Asn Tyr Gly 180 185 190 Pro Phe His Phe Pro Glu Lys Leu Ile Pro Leu Thr Ile Leu Asn Ala 195 200 205 Leu Glu Gly Lys Pro Leu Pro Val Tyr Gly Lys Gly Glu Asn Ile Arg 210 215 220 Asp Trp Leu Tyr Val Asp Asp His Ala Lys Ala Leu Ala Thr Ile Ala 225 230 235 240 Thr Thr Gly Lys Val Gly Gln Ser Tyr Asn Val Gly Gly Arg Asn Glu 245 250 255 Arg Thr Asn Leu Gln Val Val Glu Thr Ile Cys Asp Leu Leu Asp Gln 260 265 270 Arg Ile Pro Leu Ala Asp Gly Arg Lys Arg Arg Glu Leu Ile Thr Phe 275 280 285 Val Thr Asp Arg Pro Gly His Asp Arg Arg Tyr Ala Ile Asp Ala Thr 290

295 300 Lys Leu Glu Thr Glu Leu Gly Trp Lys Ala Glu Glu Asn Phe Asp Thr 305 310 315 320 Gly Ile Ala Ala Thr Ile Asp Trp Tyr Leu Ala Asn Glu Trp Trp Trp 325 330 335 Gly Pro Ile Arg Ser Gly Lys Tyr Ala Gly Glu Arg Leu Gly Gln Thr 340 345 350 Ala 42867DNASphingomonas sp. ATCC53159CDS(1)..(867) 42atg cgt atc ctc gtc acc ggg cat gac ggc cag gtc gcc cag tcg ctg 48Met Arg Ile Leu Val Thr Gly His Asp Gly Gln Val Ala Gln Ser Leu 1 5 10 15 gcc gag cag gcg gtg ggc cac gag ctg gtc ttc acc acc tac ccc gaa 96Ala Glu Gln Ala Val Gly His Glu Leu Val Phe Thr Thr Tyr Pro Glu 20 25 30 ttc gat ctc tcc aag ccg gag acg atc gag gcc ggt gtg gcg cgg gtg 144Phe Asp Leu Ser Lys Pro Glu Thr Ile Glu Ala Gly Val Ala Arg Val 35 40 45 cac ccg gac ctg atc gtc tcc gcc gcc gcc tac acg gcg gtc gac aag 192His Pro Asp Leu Ile Val Ser Ala Ala Ala Tyr Thr Ala Val Asp Lys 50 55 60 gcg gaa agc gaa ccc gag ctg gcg atg gcg atc aac ggc gac ggt ccc 240Ala Glu Ser Glu Pro Glu Leu Ala Met Ala Ile Asn Gly Asp Gly Pro 65 70 75 80 ggc gtg ctg gcg cgc gcg ggc gcg aag atc ggc gcg ccg atc atc cac 288Gly Val Leu Ala Arg Ala Gly Ala Lys Ile Gly Ala Pro Ile Ile His 85 90 95 ctg tcg acc gat tat gtg ttc gac ggc agt ctc gac cgc cct tgg cgc 336Leu Ser Thr Asp Tyr Val Phe Asp Gly Ser Leu Asp Arg Pro Trp Arg 100 105 110 gag gac gat ccc acc ggc ccg ctc ggc gtc tat ggc gcg acc aag ctg 384Glu Asp Asp Pro Thr Gly Pro Leu Gly Val Tyr Gly Ala Thr Lys Leu 115 120 125 gcc ggc gag cag gcg gtg cag gcc tcg ggt gcc acc aac gcc gtg atc 432Ala Gly Glu Gln Ala Val Gln Ala Ser Gly Ala Thr Asn Ala Val Ile 130 135 140 cgg ctg gcc tgg gtc tac agc ccg ttc ggc aac aat ttc gtc aag acg 480Arg Leu Ala Trp Val Tyr Ser Pro Phe Gly Asn Asn Phe Val Lys Thr 145 150 155 160 atg ctc cgc ctc gcc gag acg cgc gac gcg ctg aac gtc gtg gag gac 528Met Leu Arg Leu Ala Glu Thr Arg Asp Ala Leu Asn Val Val Glu Asp 165 170 175 cag tgg ggc tgc ccc agt tcg gcg ctg gac atc gcg acc gcg atc ctg 576Gln Trp Gly Cys Pro Ser Ser Ala Leu Asp Ile Ala Thr Ala Ile Leu 180 185 190 acg gtg gtc ggg cac tgg cag cag gac ggc gcg acg agc ggc ctc tac 624Thr Val Val Gly His Trp Gln Gln Asp Gly Ala Thr Ser Gly Leu Tyr 195 200 205 cat ttc gcc ggc acc ggc gag acc aac tgg gcc gac ttc gca tcg acg 672His Phe Ala Gly Thr Gly Glu Thr Asn Trp Ala Asp Phe Ala Ser Thr 210 215 220 atc ttc gcc gag agc gcc aag cgc ggt ggc ccc tcg gcc acc gtc acc 720Ile Phe Ala Glu Ser Ala Lys Arg Gly Gly Pro Ser Ala Thr Val Thr 225 230 235 240 ggc att ccc agc tcg ggc tat ccg act ccg gcc acg cgc ccg gcc aat 768Gly Ile Pro Ser Ser Gly Tyr Pro Thr Pro Ala Thr Arg Pro Ala Asn 245 250 255 tcg cgg ctg gac tgc acc cgc ttc gcg gag acc ttc ggc tac cgg gcg 816Ser Arg Leu Asp Cys Thr Arg Phe Ala Glu Thr Phe Gly Tyr Arg Ala 260 265 270 cct gcc tgg cag gat tcg ctg aac gtc gta ctg gat cgc ctg ctc ggc 864Pro Ala Trp Gln Asp Ser Leu Asn Val Val Leu Asp Arg Leu Leu Gly 275 280 285 tga 867 43288PRTSphingomonas sp. ATCC53159 43Met Arg Ile Leu Val Thr Gly His Asp Gly Gln Val Ala Gln Ser Leu 1 5 10 15 Ala Glu Gln Ala Val Gly His Glu Leu Val Phe Thr Thr Tyr Pro Glu 20 25 30 Phe Asp Leu Ser Lys Pro Glu Thr Ile Glu Ala Gly Val Ala Arg Val 35 40 45 His Pro Asp Leu Ile Val Ser Ala Ala Ala Tyr Thr Ala Val Asp Lys 50 55 60 Ala Glu Ser Glu Pro Glu Leu Ala Met Ala Ile Asn Gly Asp Gly Pro 65 70 75 80 Gly Val Leu Ala Arg Ala Gly Ala Lys Ile Gly Ala Pro Ile Ile His 85 90 95 Leu Ser Thr Asp Tyr Val Phe Asp Gly Ser Leu Asp Arg Pro Trp Arg 100 105 110 Glu Asp Asp Pro Thr Gly Pro Leu Gly Val Tyr Gly Ala Thr Lys Leu 115 120 125 Ala Gly Glu Gln Ala Val Gln Ala Ser Gly Ala Thr Asn Ala Val Ile 130 135 140 Arg Leu Ala Trp Val Tyr Ser Pro Phe Gly Asn Asn Phe Val Lys Thr 145 150 155 160 Met Leu Arg Leu Ala Glu Thr Arg Asp Ala Leu Asn Val Val Glu Asp 165 170 175 Gln Trp Gly Cys Pro Ser Ser Ala Leu Asp Ile Ala Thr Ala Ile Leu 180 185 190 Thr Val Val Gly His Trp Gln Gln Asp Gly Ala Thr Ser Gly Leu Tyr 195 200 205 His Phe Ala Gly Thr Gly Glu Thr Asn Trp Ala Asp Phe Ala Ser Thr 210 215 220 Ile Phe Ala Glu Ser Ala Lys Arg Gly Gly Pro Ser Ala Thr Val Thr 225 230 235 240 Gly Ile Pro Ser Ser Gly Tyr Pro Thr Pro Ala Thr Arg Pro Ala Asn 245 250 255 Ser Arg Leu Asp Cys Thr Arg Phe Ala Glu Thr Phe Gly Tyr Arg Ala 260 265 270 Pro Ala Trp Gln Asp Ser Leu Asn Val Val Leu Asp Arg Leu Leu Gly 275 280 285 44132DNASphingomonas sp. ATCC53159CDS(1)..(132) 44atc cgg ctg tgc ctg ggg tgc tgg cgg tcg ccc aag gaa atc gcc ggc 48Ile Arg Leu Cys Leu Gly Cys Trp Arg Ser Pro Lys Glu Ile Ala Gly 1 5 10 15 tgg agc gag ctg agt cct aag gga aag cgc gcg gtg cta gag gca ttg 96Trp Ser Glu Leu Ser Pro Lys Gly Lys Arg Ala Val Leu Glu Ala Leu 20 25 30 ccg gcg cgc gaa cgg gag cat ggc ggg ggg cgc tga 132Pro Ala Arg Glu Arg Glu His Gly Gly Gly Arg 35 40 4543PRTSphingomonas sp. ATCC53159 45Ile Arg Leu Cys Leu Gly Cys Trp Arg Ser Pro Lys Glu Ile Ala Gly 1 5 10 15 Trp Ser Glu Leu Ser Pro Lys Gly Lys Arg Ala Val Leu Glu Ala Leu 20 25 30 Pro Ala Arg Glu Arg Glu His Gly Gly Gly Arg 35 40

Claims

1-16. (canceled)

17. An isolated nucleic acid molecule which encodes at least one diutan biosynthetic enzyme as shown in SEQ ID NO: 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, and 43, or an enzyme which is at least 95% identical to SEQ ID NO: 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, and 43.

18. The isolated nucleic acid molecule of claim 17 which encodes a diutan polymerase.

19. The isolated nucleic acid molecule of claim 17 which encodes a diutan polymerase and a polysaccharide export protein.

20. The isolated nucleic acid molecule of claim 17 which encodes a diutan polymerase and a rhamnosyl transferase IV; a glucosyl-isoprenylphosphate transferase I; a beta-1,4-glucuronosyl transferase II; and a glucosyl transferase III.

21. The isolated nucleic acid molecule of claim 17 which encodes a diutan polymerase and a glucose-1-phosphate thyrnidylyltransferase; a dTDP-6-deoxy-D-glucose-3-5-epimerase; a dTDP-D-glucose-4,6-dehydratase; and a dTDP-6-deoxy-L-mannose-dehydrogenase.

22. The isolated nucleic acid molecule of claim 17 which encodes a diutan polymerase; lyase; rhamnosyl transferase IV; beta-1,4-glucuronosyl transferase II; glucosyl transferase III; polysaccharide export protein; secretion protein; glucosyl-isoprenylphosphate transferase I; glucose-1-phosphate thymidylyltransferase; dTDP-6-deoxy-D-glucose-3-5-epimerase; dTDP-D-glucose-4,6-dehydratase; and dTDP-6-deoxy-L-mannose-dehydrogenase.

23. The isolated nucleic acid molecule of claim 17 which comprises the nucleic acid sequence according to SEQ ID NO: 1.

24. A Sphingomonas strain comprising a nucleic acid molecule according to claim 17.

25. The strain of claim 19 which comprises the nucleic acid sequence according to SEQ ID NO:1.

Images