CANDIDA TROPICALIS CELLS AND USE THEREOF

Abstract

genetically engineered Candida tropicalis cells, use thereof and a method of production of ω-hydroxycarboxylic acids and ω-hydroxycarboxylic acid esters.

Description

FIELD OF THE INVENTION

[0001] The invention relates to genetically engineered Candida tropicalis cells, use thereof and a method of production of ω-hydroxycarboxylic acids and ω-hydroxycarboxylic acid esters.

BACKGROUND OF THE INVENTION

[0002] Owing to its ability to form dicarboxylic acids from alkanes, Candida tropicalis is a well-characterized ascomycete.

[0003] WO91/006660 describes Candida tropicalis cells that are completely inhibited in β-oxidation through interruption of the POX4 and/or POX5 genes, and achieve increased yields of α,ω-dicarboxylic acids.

[0004] WO00/020566 describes cytochrome P450 monooxygenases and NADPH cytochrome P450 oxidoreductases from Candida tropicalis and use thereof for influencing ω-hydroxylation, the first step in ω-oxidation.

[0005] WO03/089610 describes enzymes from Candida tropicalis which catalyse the second step of ω-oxidation, the conversion of a fatty alcohol to an aldehyde, and use thereof for improved production of dicarboxylic acids.

[0006] The cells and methods described so far are not suitable for the production of ω-hydroxycarboxylic acids or their esters, as the ω-hydroxycarboxylic acids are always only present as a short-lived intermediate and are immediately metabolized further.

[0007] ω-Hydroxycarboxylic acids and their esters are economically important compounds as precursors of polymers, and this forms the basis of the commercial usability of the present invention.

[0008] The task of the invention was to find a way of preparing ω-hydroxycarboxylic acids or ω-hydroxycarboxylic acid esters by fermentation in sufficient amounts, in particular in the medium surrounding the cells.

DESCRIPTION OF THE INVENTION

[0009] It was found, surprisingly, that the cells described hereunder make a contribution to solution of this task.

[0010] The object of the present invention is therefore a cell as described in claim 1.

[0011] Another object of the invention is the use of the cell according to the invention and a method of production of ω-hydroxycarboxylic acids and ω-hydroxycarboxylic acid esters using the cells according to the invention.

[0012] Advantages of the invention are the gentle conversion of the educt used to the ω-hydroxycarboxylic acids and corresponding esters and a high specificity of the method and an associated high yield based on the educt used.

[0013] One object of the present invention is a Candida tropicalis cell, in particular one from the strain ATCC 20336, which is characterized in that the cell has, compared with its wild type, a reduced activity of at least one of the enzymes that are encoded by the intron-free nucleic acid sequences selected from the two group comprising

A) Seq ID No. 1, Seq ID No. 3, Seq ID No. 5, Seq ID No. 7, Seq ID No. 9, Seq ID No. 11, Seq ID No. 13, Seq ID No. 15, Seq ID No. 17, Seq ID No. 19, Seq ID No. 21, Seq ID No. 23, Seq ID No. 25, Seq ID No. 27, Seq ID No. 29, Seq ID No. 31, Seq ID No. 33, Seq ID No. 35, Seq ID No. 37, Seq ID No. 39, Seq ID No. 41, Seq ID No. 43, Seq ID No. 45, Seq ID No. 47, Seq ID No. 49, Seq ID No. 51, Seq ID No. 53, Seq ID No. 55, Seq ID No. 57, Seq ID No. 59, Seq ID No. 61, Seq ID No. 63, Seq ID No. 65 and Seq ID No. 67; in particular Seq ID No. 1, Seq ID No. 3, Seq ID No. 5, Seq ID No. 7, Seq ID No. 9, Seq ID No. 11, Seq ID No. 13, Seq ID No. 15, Seq ID No. 17, Seq ID No. 19, Seq ID No. 21, Seq ID No. 23, Seq ID No. 25, Seq ID No. 27, Seq ID No. 29, Seq ID No. 31, Seq ID No. 33, Seq ID No. 35, Seq ID No. 37, Seq ID No. 39, Seq ID No. 41, Seq ID No. 43, Seq ID No. 45, Seq ID No. 47, Seq ID No. 49 and Seq ID No. 51; quite especially Seq ID No. 1, Seq ID No. 3, Seq ID No. 5, Seq ID No. 7, Seq ID No. 9, Seq ID No. 11, Seq ID No. 13, Seq ID No. 15, Seq ID No. 17, Seq ID No. 19, Seq ID No. 21, Seq ID No. 23, Seq ID No. 25 and Seq ID No. 27, B) a sequence that is identical to at least 80%, especially preferably to at least 90%, even more preferably to at least 95% and most preferably to at least 99% to one of the sequences Seq ID No. 1, Seq ID No. 3, Seq ID No. 5, Seq ID No. 7, Seq ID No. 9, Seq ID No. 11, Seq ID No. 13, Seq ID No. 15, Seq ID No. 17, Seq ID No. 19, Seq ID No. 21, Seq ID No. 23, Seq ID No. 25, Seq ID No. 27, Seq ID No. 29, Seq ID No. 31, Seq ID No. 33, Seq ID No. 35, Seq ID No. 37, Seq ID No. 39, Seq ID No. 41, Seq ID No. 43, Seq ID No. 45, Seq ID No. 47, Seq ID No. 49, Seq ID No. 51, Seq ID No. 53, Seq ID No. 55, Seq ID No. 57, Seq ID No. 59, Seq ID No. 61, Seq ID No. 63, Seq ID No. 65 and Seq ID No. 67; in particular to Seq ID No. 1, Seq ID No. 3, Seq ID No. 5, Seq ID No. 7, Seq ID No. 9, Seq ID No. 11, Seq ID No. 13, Seq ID No. 15, Seq ID No. 17, Seq ID No. 19, Seq ID No. 21, Seq ID No. 23, Seq ID No. 25, Seq ID No. 27, Seq ID No. 29, Seq ID No. 31, Seq ID No. 33, Seq ID No. 35, Seq ID No. 37, Seq ID No. 39, Seq ID No. 41, Seq ID No. 43, Seq ID No. 45, Seq ID No. 47, Seq ID No. 49 and Seq ID No. 51; quite especially to Seq ID No. 1, Seq ID No. 3, Seq ID No. 5, Seq ID No. 7, Seq ID No. 9, Seq ID No. 11, Seq ID No. 13, Seq ID No. 15, Seq ID No. 17, Seq ID No. 19, Seq ID No. 21, Seq ID No. 23, Seq ID No. 25 and Seq ID No. 27.

[0014] In this connection, the nucleic acid sequence group that is preferred according to the invention is group A).

[0015] A "wild type" of a cell preferably means, in connection with the present invention, the starting strain from which the cell according to the invention was derived by manipulation of the elements (for example the genes comprising the aforesaid nucleic acid sequences coding for a corresponding enzyme or the promoters contained in the corresponding gene, which are linked functionally with the aforesaid nucleic acid sequences), which influence the activities of the enzymes encoded by the stated nucleic acid Seq ID No. If for example the activity of the enzyme encoded by Seq ID No. 1 in the strain ATCC 20336 is reduced by interruption of the corresponding gene, then the strain ATCC 20336 that is unchanged and was used for the corresponding manipulation is to be regarded as the "wild type".

[0016] The term "gene" means, in connection with the present invention, not only the encoding DNA region or that transcribed to mRNA, the "structural gene", but in addition promoter, possible intron, enhancer and other regulatory sequence, and terminator, regions.

[0017] The term "activity of an enzyme" always means, in connection with the invention, the enzymatic activity that catalyses the reactions of 12-hydroxydodecanoic acid to 1,12-dodecane diacid by the entire cell. This activity is preferably determined by the following method:

[0018] Starting from a single colony, a 100-ml Erlenmeyer flask with 10 ml of YM medium (0.3% yeast extract, 0.3% malt extract, 0.5% peptone and 1.0% (w/w) glucose) is cultivated at 30° C. and 90 rpm for 24 h. Then, starting from this culture, 10 ml is inoculated into a 1-litre Erlenmeyer flask with 100 ml of production medium (for 1 litre: 25 g glucose, 7.6 g NH₄Cl, 1.5 g Na₂SO₄, 300 ml of a 1 mM potassium phosphate buffer (pH 7.0), 20 mg ZnSO₄×7H₂O, 20 mg MnSO₄×4H₂O, 20 mg nicotinic acid, 20 mg pyridoxine, 8 mg thiamine and 6 mg pantothenate). It is cultivated for 24 h at 30° C.

[0019] After 24 h, 12-hydroxydodecanoic acid is added to the cell suspension, so that the concentration is not greater than 0.5 g/l. Glucose or glycerol is also added as co-substrate, so that the concentration of the co-substrate does not drop below 0.2 g/l. After 0 h, 0.5 h, 1 h, and then hourly up to a cultivation time of 24 h, samples (1 ml) are taken for measurement of 12-hydroxydodecanoic acid, 12-oxo-dodecanoic acid and 1,12-dodecane diacid and the corresponding methyl esters, and for checking the cell count. After each measurement, the pH is kept between 5.0 and 6.5 with 6N NaOH or 4NH₂SO₄. During cultivation, cell growth is verified by checking the "colony forming units" (CFU). The decrease of 12-hydroxydodecanoic acid and the production of 1,12-dodecane diacid or the corresponding methyl esters are verified by LC-MS. For this, 500 μl of culture broth is adjusted to pH 1 and then extracted with the same volume of diethyl ether or ethyl acetate and analysed by LC-MS.

[0020] The measuring system consists of an HP1100 HPLC (Agilent Technologies, Waldbronn, Germany) with degasser, autosampler and column furnace, coupled to a mass-selective quadrupole detector MSD (Agilent Technologies, Waldbronn, Germany). Chromatographic separation is achieved on a reversed phase e.g. 125×2 mm Luna C18(2) column (Phenomenex, Aschaffenburg, Germany) at 40° C. Gradient elution is performed at a flow of 0.3 ml/min (A: 0.02% formic acid in water and B: 0.02% formic acid in acetonitrile). Alternatively, the organic extracts are analysed by GC-FID (Perkin Elmer, Rodgau-Jugesheim, Germany). Chromatographic separation is performed on a methylpolysiloxane (5% phenyl) phase e.g. Elite 5, 30 m, 0.25 mm ID, 0.25 μm FD (Perkin Elmer, Rodgau-Jugesheim, Germany). Before measurement, a methylation reagent e.g. trimethylsulphonium hydroxide "TMSH" (Macherey-Nagel GmbH & Co. KG, Duren, Germany) is added to free acids and on injection they are converted to the corresponding methyl esters.

[0021] By calculating the measured concentration of 1,12-dodecane diacid and the cell number at the time of sampling, it is possible to determine the specific production rate of 1,12-dodecane diacid from 12-hydroxydodecanoic acid and therefore the "activity of an enzyme" in a cell as defined above. The formulation "reduced activity compared with its wild type" means an activity relative to the wild-type activity preferably reduced by at least 50%, especially preferably by at least 90%, more preferably by at least 99.9%, even more preferably by at least 99.99% and most preferably by at least 99.999%.

[0022] The decrease in activity of the cell according to the invention compared with its wild type is determined by the method described above for determining activity using cell numbers/concentrations as identical as possible, the cells having been grown under the same conditions, for example medium, gassing, agitation.

[0023] "Nucleotide identity" relative to the stated sequences can be determined using known methods. Generally, special computer programs are used with algorithms taking special requirements into account. Preferred methods for determining identity first produce the greatest agreement between the sequences to be compared. Computer programs for determining identity comprise, but are not restricted to, the GCG software package, including [0024] GAP (Deveroy, J. et al., Nucleic Acid Research 12 (1984), page 387, Genetics Computer Group University of Wisconsin, Medicine (Wi), and [0025] BLASTP, BLASTN and FASTA (Altschul, S. et al., Journal of Molecular Biology 215 (1990), pages 403-410. The BLAST program can be obtained from the National Center for Biotechnology Information (NCBI) and from other sources (BLAST Manual, Altschul S. et al., NCBI NLM NIH Bethesda N. Dak. 22894; Altschul S. et al., above).

[0026] The known Smith-Waterman algorithm can also be used for determining nucleotide identity.

[0027] Preferred parameters for the determination of "nucleotide identity" are, when using the BLASTN program (Altschul, S. et al., Journal of Molecular Biology 215 (1990), pages 403-410):

TABLE-US-00001 Expect Threshold: 10 Word size: 28 Match score: 1 Mismatch score: -2 Gap costs: linear

[0028] The above parameters are the default parameters in nucleotide sequence comparison.

[0029] The GAP program is also suitable for use with the above parameters.

[0030] An identity of 80% according to the above algorithm means, in connection with the present invention, 80% identity. The same applies to higher identities.

[0031] The term "that are encoded by the intron-free nucleic acid sequences" makes clear that in a sequence comparison with the sequences given here, the nucleic acid sequences to be compared must be purified of any introns beforehand.

[0032] All stated percentages (%) are percentages by weight unless stated otherwise.

[0033] Methods of lowering enzymatic activities in microorganisms are known by a person skilled in the art.

[0034] In particular, techniques in molecular biology can be used for this. A person skilled in the art can find instructions on modification and decrease of protein expression and the associated decrease in enzyme activity especially for Candida tropicalis, in particular for interrupting specified genes, in WO91/006660; WO03/100013; Picataggio et al. Mol Cell Biol. 1991 September; 11(9):4333-9; Rohrer et al. Appl Microbiol Biotechnol. 1992 February; 36(5):650-4; Picataggio et al. Biotechnology (N Y). 1992 August; 10(8):894-8; Ueda et al. Biochim Biophys Acta. 2003 Mar. 17; 1631(2):160-8; Ko et al. Appl Environ Microbiol. 2006 June; 72(6):4207-13; Hara et al. Arch Microbiol. 2001 November; 176(5):364-9; Kanayama et al. J Bacteriol. 1998 February; 180(3): 690-8.

[0035] Cells preferred according to the invention are characterized in that the decrease in enzymatic activity is achieved by modification of at least one gene comprising one of the sequences selected from the previously stated nucleic acid sequence groups A) and B), the modification being selected from the group comprising, preferably consisting of, insertion of foreign DNA into the gene, deletion at least of parts of the gene, point mutations in the gene sequence and subjecting the gene to the influence of RNA interference or exchange of parts of the gene with foreign DNA, in particular of the promoter region.

[0036] Foreign DNA means, in this context, any DNA sequence that is "foreign" to the gene (and not to the organism), i.e. even Candida tropicalis endogenous DNA sequences can, in this context, function as "foreign DNA".

[0037] In this context, it is in particular preferable for the gene to be interrupted by insertion of a selection marker gene, therefore the foreign DNA is a selection marker gene, the insertion preferably having been effected by homologous recombination into the gene locus.

[0038] In this context, it may be advantageous if the selection marker gene is expanded with further functionalities, which in their turn make subsequent removal from the gene possible, this can be achieved for example with a Cre/loxP system, with Flippase Recognition Targets (FRT) or by homologous recombination.

[0039] Cells preferred according to the invention are characterized in that they are blocked in their β-oxidation at least partially, preferably completely, as this prevents outflow of substrate and therefore higher titres become possible.

[0040] Examples of Candida tropicalis cells partially blocked in their β-oxidation are described in EP0499622 as strains H41, H41B, H51, H45, H43, H53, H534, H534B and H435, from which a Candida tropicalis cell preferred according to the invention is derived.

[0041] Other Candida tropicalis cells blocked for β-oxidation are described for example in WO03/100013.

[0042] In this context, cells are preferred for which the β-oxidation is caused by an induced malfunction of at least one of the genes POX2, POX4 or POX5.

[0043] Therefore, in this context, cells are preferred that are characterized in that a Candida tropicalis cell preferred according to the invention is derived from strains selected from the group comprising ATCC 20962 and the Candida tropicalis HDC100 described in US2004/0014198.

[0044] The use of the cells according to the invention for the production of ω-hydroxycarboxylic acids or ω-hydroxycarboxylic acid esters also contributes to solution of the task facing the invention.

[0045] In particular, the use of the cells according to the invention for the production of ω-hydroxycarboxylic acids or ω-hydroxycarboxylic acid esters with a chain length of the carboxylic acid from 6 to 24, preferably 8 to 18 and especially preferably 10 to 16 carbon atoms, which are preferably linear, saturated and unsubstituted, and a chain length of the alcohol component of the ester from 1 to 4, in particular 1 or 2 carbon atoms, is advantageous. In this context, it is preferable for the ω-hydroxycarboxylic acids to be 12-hydroxydodecanoic acid and for the ω-hydroxycarboxylic acid ester to be 12-hydroxydodecanoic acid methyl ester.

[0046] A preferred use is characterized according to the invention in that preferred cells according to the invention as described above are used.

[0047] Another contribution to solving the task facing the invention is made by a method of production of the C. tropicalis cell according to the invention described above comprising the steps:

I) Preparation of a C. tropicalis cell, preferably a cell that is blocked in its β-oxidation at least partially, preferably completely II) Modification of at least one gene comprising one of the intron-free nucleic acid sequences selected from the previously stated nucleic acid sequence groups A) and B) by insertion of foreign DNA, in particular of DNA coding for a selection marker gene, into the gene, deletion of at least parts of the gene, point mutations in the gene sequence and subjecting the gene to the influence of RNA interference or exchange of parts of the gene with foreign DNA, in particular of the promoter region.

[0048] Another contribution to solving the task facing the invention is made by a method of production of ω-hydroxycarboxylic acids or ω-hydroxycarboxylic acid esters, in particular of ω-hydroxycarboxylic acids or ω-hydroxycarboxylic acid esters with a chain length of the carboxylic acid from 6 to 24, preferably 8 to 18 and especially preferably 10 to 16 carbon atoms, which are preferably linear, saturated and unsubstituted, and a chain length of the alcohol component of the ester from 1 to 4, in particular of 1 or 2 carbon atoms, in particular of 12-hydroxydodecanoic acid or 12-hydroxydodecanoic acid methyl ester comprising the steps

A) contacting a previously described cell according to the invention with a medium comprising a carboxylic acid or a carboxylic acid ester, in particular a carboxylic acid or a carboxylic acid ester with a chain length of the carboxylic acid from 6 to 24, preferably 8 to 18 and especially preferably 10 to 16 carbon atoms, which are preferably linear, saturated and unsubstituted, and a chain length of the alcohol component of the ester from 1 to 4 carbon atoms, in particular dodecanoic acid or dodecanoic acid methyl ester, B) cultivating the cell under conditions that enable the cell to form the corresponding ω-hydroxycarboxylic acid or ω-hydroxycarboxylic acid esters from the carboxylic acid or the carboxylic acid ester and C) optionally isolating the ω-hydroxycarboxylic acid or ω-hydroxycarboxylic acid esters that formed.

[0049] Preferred methods according to the invention use cells stated above as being preferred according to the invention.

[0050] Therefore, for example a method of production of 12-hydroxydodecanoic acid or 12-hydroxydodecanoic acid methyl ester comprising the steps

a) contacting a Candida tropicalis cell of the strain ATTC 20336 at least partially blocked in its β-oxidation, which has, compared with its wild type, a reduced activity of at least one of the enzymes, which are encoded by the intron-free nucleic acid sequences selected from the previously stated nucleic acid sequence groups A) and B), the decrease in enzymatic activity being achieved by modification of a gene comprising one of the nucleic acid sequences selected from the previously stated nucleic acid sequence groups A) and B), wherein the modification consists of insertion of a selection marker gene into the gene, with a medium comprising dodecanoic acid or dodecanoic acid methyl ester, b) cultivating the cell under conditions that enable the cell to form the corresponding ω-hydroxycarboxylic acid or ω-hydroxycarboxylic acid esters from the carboxylic acid or the carboxylic acid ester and c) optionally isolating the ω-hydroxycarboxylic acid or ω-hydroxycarboxylic acid esters that formed is quite especially preferred.

[0051] Suitable cultivation conditions for Candida tropicalis are known by a person skilled in the art. In particular, suitable conditions for step b) are those that are known by a person skilled in the art from bioconversion methods of production of dicarboxylic acids with Candida tropicalis.

[0052] These cultivation conditions are described for example in WO00/017380 and WO00/015828.

[0053] Methods for isolating the ω-hydroxycarboxylic acid or ω-hydroxycarboxylic acid esters that formed are known by a person skilled in the art. These are standard methods for isolating long-chain carboxylic acids from aqueous solution, for example distillation or extraction, and can for example also be found in WO2009/077461.

[0054] It is advantageous to use the ω-hydroxycarboxylic acids or ω-hydroxycarboxylic acid esters obtained by the method according to the invention for the production of polymers, in particular polyesters. Moreover, lactones can also be produced from the ω-hydroxy carboxylic acids, and can then for example be used in their turn for the production of polyesters.

[0055] Another advantageous use is to convert the ω-hydroxycarboxylic acids or ω-hydroxycarboxylic acid esters to ω-aminocarboxylic acids or ω-aminocarboxylic acid esters, in order to obtain polyamides as polymers. The ω-aminocarboxylic acids or ω-aminocarboxylic acid esters can also be converted first to the corresponding lactams, which can then in their turn be converted using anionic, or also acid catalysis to a polyamide.

[0056] It is quite especially advantageous, in a first reaction step, to convert the ω-hydroxycarboxylic acids or corresponding esters into the ω-oxo-carboxylic acids or the corresponding esters and then to carry out amination of the oxo-group, e.g. in the course of reductive amination.

[0057] In this context, the use of 12-hydroxy dodecanoic acid or 12-hydroxydodecanoic acid methyl ester for the production of polymers, in particular of polyamide 12, is especially preferred.

Sequence CWU 1

6811575DNACandida tropicalisCDS(1)..(1572) 1atg gcc aca caa gaa att att gat tct gca ctt ccg tac ttg aca aag 48Met Ala Thr Gln Glu Ile Ile Asp Ser Ala Leu Pro Tyr Leu Thr Lys1 5 10 15tgg tat act gtt atc act tta gca gct ttg gtt ttc tta att tca tct 96Trp Tyr Thr Val Ile Thr Leu Ala Ala Leu Val Phe Leu Ile Ser Ser 20 25 30aat att aaa aat tac gtc aag gct aag aag ttg aaa tgc aga gat cct 144Asn Ile Lys Asn Tyr Val Lys Ala Lys Lys Leu Lys Cys Arg Asp Pro 35 40 45cca tat ttc aaa gga gcc ggt tgg aca ggt att agt cca tta att gaa 192Pro Tyr Phe Lys Gly Ala Gly Trp Thr Gly Ile Ser Pro Leu Ile Glu 50 55 60att att aaa gtt aaa ggt aat ggt aga ttg gca aga ttt tgg ccg ata 240Ile Ile Lys Val Lys Gly Asn Gly Arg Leu Ala Arg Phe Trp Pro Ile65 70 75 80aaa aca ttc gac gac tat cca aac cat act ttt tac atg tct att att 288Lys Thr Phe Asp Asp Tyr Pro Asn His Thr Phe Tyr Met Ser Ile Ile 85 90 95ggt gct ttg aaa atc gtc ttg act gtg atc caa gaa aat att aaa gct 336Gly Ala Leu Lys Ile Val Leu Thr Val Ile Gln Glu Asn Ile Lys Ala 100 105 110gtt ttg gct act caa ttt act gat ttc tcc tta ggt act aga cat gcc 384Val Leu Ala Thr Gln Phe Thr Asp Phe Ser Leu Gly Thr Arg His Ala 115 120 125cat ttc tat cca tta tta ggt gat ggt att ttt act ttg gat ggt gaa 432His Phe Tyr Pro Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Glu 130 135 140ggt tgg aaa cat agt aga gct atg ttg aga cca caa ttt gct aga gat 480Gly Trp Lys His Ser Arg Ala Met Leu Arg Pro Gln Phe Ala Arg Asp145 150 155 160caa att ggt cat gtt aaa gct ttg gaa cca cat att caa atc ttg gct 528Gln Ile Gly His Val Lys Ala Leu Glu Pro His Ile Gln Ile Leu Ala 165 170 175aaa caa atc aaa ttg aat aaa ggt aaa act ttt gat att caa gaa ttg 576Lys Gln Ile Lys Leu Asn Lys Gly Lys Thr Phe Asp Ile Gln Glu Leu 180 185 190ttt ttc aga ttt act gtt gat act gct act gaa ttc ttg ttt ggt gaa 624Phe Phe Arg Phe Thr Val Asp Thr Ala Thr Glu Phe Leu Phe Gly Glu 195 200 205tct gtt cac tct ttg tat gat gaa aaa tta ggt att cct act cca aat 672Ser Val His Ser Leu Tyr Asp Glu Lys Leu Gly Ile Pro Thr Pro Asn 210 215 220gaa att cca ggt aga gat aat ttt gca act gct ttt aac act tct caa 720Glu Ile Pro Gly Arg Asp Asn Phe Ala Thr Ala Phe Asn Thr Ser Gln225 230 235 240cat tat ttg gct acc aga aca tac tcc caa act ttc tac ttt tta act 768His Tyr Leu Ala Thr Arg Thr Tyr Ser Gln Thr Phe Tyr Phe Leu Thr 245 250 255aac cct aag gaa ttt aga gac tgt aat gct aaa gtt cat tac ttg gct 816Asn Pro Lys Glu Phe Arg Asp Cys Asn Ala Lys Val His Tyr Leu Ala 260 265 270aaa tat ttt gtc aat aaa gct ttg aat ttc act ccg gaa gaa att gaa 864Lys Tyr Phe Val Asn Lys Ala Leu Asn Phe Thr Pro Glu Glu Ile Glu 275 280 285gaa aag tcc aaa tct ggt tat gtt ttc ttg tat gaa ttg gtt aaa caa 912Glu Lys Ser Lys Ser Gly Tyr Val Phe Leu Tyr Glu Leu Val Lys Gln 290 295 300acc aga gat cca aaa gtt tta caa gat caa tta ttg aac att atg gtt 960Thr Arg Asp Pro Lys Val Leu Gln Asp Gln Leu Leu Asn Ile Met Val305 310 315 320gcc ggt aga gat acc act gct ggt tta tta tca ttt gca atg ttt gaa 1008Ala Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Ala Met Phe Glu 325 330 335tta gct aga cat cca gaa att tgg tct aaa tta aga gaa gaa att gaa 1056Leu Ala Arg His Pro Glu Ile Trp Ser Lys Leu Arg Glu Glu Ile Glu 340 345 350gtt aac ttt ggt gtt ggt gaa gaa tct cgt gtt gaa gaa att act ttt 1104Val Asn Phe Gly Val Gly Glu Glu Ser Arg Val Glu Glu Ile Thr Phe 355 360 365gaa tct ttg aag aga tgt gaa tac ttg aaa gct att ctt aat gaa act 1152Glu Ser Leu Lys Arg Cys Glu Tyr Leu Lys Ala Ile Leu Asn Glu Thr 370 375 380ttg cgt atg tat cct tct gtt cca gtt aat tcc aga aca gcc act aga 1200Leu Arg Met Tyr Pro Ser Val Pro Val Asn Ser Arg Thr Ala Thr Arg385 390 395 400gat acc aca tta cca aga ggt ggt ggt cca aat ggt act gat cca att 1248Asp Thr Thr Leu Pro Arg Gly Gly Gly Pro Asn Gly Thr Asp Pro Ile 405 410 415ttt att cca aag ggt tcc act gtt gct tat att gtt tac aaa act cat 1296Phe Ile Pro Lys Gly Ser Thr Val Ala Tyr Ile Val Tyr Lys Thr His 420 425 430cgt tta gaa gaa tat tat ggt aaa gat gct gat gat ttc aga cca gaa 1344Arg Leu Glu Glu Tyr Tyr Gly Lys Asp Ala Asp Asp Phe Arg Pro Glu 435 440 445aga tgg ttt gaa cca tca act aaa aag tta ggt tgg gct tat gtt cca 1392Arg Trp Phe Glu Pro Ser Thr Lys Lys Leu Gly Trp Ala Tyr Val Pro 450 455 460ttt aat ggt ggt cca aga att tgt tta ggc caa caa ttt gct tta act 1440Phe Asn Gly Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe Ala Leu Thr465 470 475 480gaa gct tct tat gtt att acc aga ttg gta caa atg ttt gaa act gtt 1488Glu Ala Ser Tyr Val Ile Thr Arg Leu Val Gln Met Phe Glu Thr Val 485 490 495tct tct ccc cca gat gtt gaa tac cct cca cca aaa tgt att cat ttg 1536Ser Ser Pro Pro Asp Val Glu Tyr Pro Pro Pro Lys Cys Ile His Leu 500 505 510act atg agt cat gat gat ggt gtt ttc gtt aaa atg taa 1575Thr Met Ser His Asp Asp Gly Val Phe Val Lys Met 515 5202524PRTCandida tropicalis 2Met Ala Thr Gln Glu Ile Ile Asp Ser Ala Leu Pro Tyr Leu Thr Lys1 5 10 15Trp Tyr Thr Val Ile Thr Leu Ala Ala Leu Val Phe Leu Ile Ser Ser 20 25 30Asn Ile Lys Asn Tyr Val Lys Ala Lys Lys Leu Lys Cys Arg Asp Pro 35 40 45Pro Tyr Phe Lys Gly Ala Gly Trp Thr Gly Ile Ser Pro Leu Ile Glu 50 55 60Ile Ile Lys Val Lys Gly Asn Gly Arg Leu Ala Arg Phe Trp Pro Ile65 70 75 80Lys Thr Phe Asp Asp Tyr Pro Asn His Thr Phe Tyr Met Ser Ile Ile 85 90 95Gly Ala Leu Lys Ile Val Leu Thr Val Ile Gln Glu Asn Ile Lys Ala 100 105 110Val Leu Ala Thr Gln Phe Thr Asp Phe Ser Leu Gly Thr Arg His Ala 115 120 125His Phe Tyr Pro Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Glu 130 135 140Gly Trp Lys His Ser Arg Ala Met Leu Arg Pro Gln Phe Ala Arg Asp145 150 155 160Gln Ile Gly His Val Lys Ala Leu Glu Pro His Ile Gln Ile Leu Ala 165 170 175Lys Gln Ile Lys Leu Asn Lys Gly Lys Thr Phe Asp Ile Gln Glu Leu 180 185 190Phe Phe Arg Phe Thr Val Asp Thr Ala Thr Glu Phe Leu Phe Gly Glu 195 200 205Ser Val His Ser Leu Tyr Asp Glu Lys Leu Gly Ile Pro Thr Pro Asn 210 215 220Glu Ile Pro Gly Arg Asp Asn Phe Ala Thr Ala Phe Asn Thr Ser Gln225 230 235 240His Tyr Leu Ala Thr Arg Thr Tyr Ser Gln Thr Phe Tyr Phe Leu Thr 245 250 255Asn Pro Lys Glu Phe Arg Asp Cys Asn Ala Lys Val His Tyr Leu Ala 260 265 270Lys Tyr Phe Val Asn Lys Ala Leu Asn Phe Thr Pro Glu Glu Ile Glu 275 280 285Glu Lys Ser Lys Ser Gly Tyr Val Phe Leu Tyr Glu Leu Val Lys Gln 290 295 300Thr Arg Asp Pro Lys Val Leu Gln Asp Gln Leu Leu Asn Ile Met Val305 310 315 320Ala Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Ala Met Phe Glu 325 330 335Leu Ala Arg His Pro Glu Ile Trp Ser Lys Leu Arg Glu Glu Ile Glu 340 345 350Val Asn Phe Gly Val Gly Glu Glu Ser Arg Val Glu Glu Ile Thr Phe 355 360 365Glu Ser Leu Lys Arg Cys Glu Tyr Leu Lys Ala Ile Leu Asn Glu Thr 370 375 380Leu Arg Met Tyr Pro Ser Val Pro Val Asn Ser Arg Thr Ala Thr Arg385 390 395 400Asp Thr Thr Leu Pro Arg Gly Gly Gly Pro Asn Gly Thr Asp Pro Ile 405 410 415Phe Ile Pro Lys Gly Ser Thr Val Ala Tyr Ile Val Tyr Lys Thr His 420 425 430Arg Leu Glu Glu Tyr Tyr Gly Lys Asp Ala Asp Asp Phe Arg Pro Glu 435 440 445Arg Trp Phe Glu Pro Ser Thr Lys Lys Leu Gly Trp Ala Tyr Val Pro 450 455 460Phe Asn Gly Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe Ala Leu Thr465 470 475 480Glu Ala Ser Tyr Val Ile Thr Arg Leu Val Gln Met Phe Glu Thr Val 485 490 495Ser Ser Pro Pro Asp Val Glu Tyr Pro Pro Pro Lys Cys Ile His Leu 500 505 510Thr Met Ser His Asp Asp Gly Val Phe Val Lys Met 515 52031572DNACandida tropicalisCDS(1)..(1569) 3atg gcc aca caa gaa atc atc gat tct gta ctt ccg tac ttg acc aaa 48Met Ala Thr Gln Glu Ile Ile Asp Ser Val Leu Pro Tyr Leu Thr Lys1 5 10 15tgg tac act gtg att act gca gca gta tta gtc ttc ctt atc tcc aca 96Trp Tyr Thr Val Ile Thr Ala Ala Val Leu Val Phe Leu Ile Ser Thr 20 25 30aac atc aag aac tac gtc aag gca aag aaa ttg aaa tgt gtc gat cca 144Asn Ile Lys Asn Tyr Val Lys Ala Lys Lys Leu Lys Cys Val Asp Pro 35 40 45cca tac ttg aag gat gcc ggt ctc act ggt att ctg tct ttg atc gcc 192Pro Tyr Leu Lys Asp Ala Gly Leu Thr Gly Ile Leu Ser Leu Ile Ala 50 55 60gcc atc aag gcc aag aac gac ggt aga ttg gct aac ttt gcc gat gaa 240Ala Ile Lys Ala Lys Asn Asp Gly Arg Leu Ala Asn Phe Ala Asp Glu65 70 75 80gtt ttc gac gag tac cca aac cac acc ttc tac ttg tct gtt gcc ggt 288Val Phe Asp Glu Tyr Pro Asn His Thr Phe Tyr Leu Ser Val Ala Gly 85 90 95gct ttg aag att gtc atg act gtt gac cca gaa aac atc aag gct gtc 336Ala Leu Lys Ile Val Met Thr Val Asp Pro Glu Asn Ile Lys Ala Val 100 105 110ttg gcc acc caa ttc act gac ttc tcc ttg ggt acc aga cac gcc cac 384Leu Ala Thr Gln Phe Thr Asp Phe Ser Leu Gly Thr Arg His Ala His 115 120 125ttt gct cct ttg ttg ggt gac ggt atc ttc acc ttg gac gga gaa ggt 432Phe Ala Pro Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Glu Gly 130 135 140tgg aag cac tcc aga gct atg ttg aga cca cag ttt gct aga gac cag 480Trp Lys His Ser Arg Ala Met Leu Arg Pro Gln Phe Ala Arg Asp Gln145 150 155 160att gga cac gtt aaa gcc ttg gaa cca cac atc caa atc atg gct aag 528Ile Gly His Val Lys Ala Leu Glu Pro His Ile Gln Ile Met Ala Lys 165 170 175cag atc aag ttg aac cag gga aag act ttc gat atc caa gaa ttg ttc 576Gln Ile Lys Leu Asn Gln Gly Lys Thr Phe Asp Ile Gln Glu Leu Phe 180 185 190ttt aga ttt acc gtc gac acc gct act gag ttc ttg ttt ggt gaa tcc 624Phe Arg Phe Thr Val Asp Thr Ala Thr Glu Phe Leu Phe Gly Glu Ser 195 200 205gtt cac tcc ttg tac gat gaa aaa ttg ggc atc cca act cca aac gaa 672Val His Ser Leu Tyr Asp Glu Lys Leu Gly Ile Pro Thr Pro Asn Glu 210 215 220atc cca gga aga gaa aac ttt gcc gct gct ttc aac gtt tcc caa cac 720Ile Pro Gly Arg Glu Asn Phe Ala Ala Ala Phe Asn Val Ser Gln His225 230 235 240tac ttg gcc acc aga agt tac tcc cag act ttt tac ttt ttg acc aac 768Tyr Leu Ala Thr Arg Ser Tyr Ser Gln Thr Phe Tyr Phe Leu Thr Asn 245 250 255cct aag gaa ttc aga gac tgt aac gcc aag gtc cac cac ttg gcc aag 816Pro Lys Glu Phe Arg Asp Cys Asn Ala Lys Val His His Leu Ala Lys 260 265 270tac ttt gtc aac aag gcc ttg aac ttt act cct gaa gaa ctc gaa gag 864Tyr Phe Val Asn Lys Ala Leu Asn Phe Thr Pro Glu Glu Leu Glu Glu 275 280 285aaa tcc aag tcc ggt tac gtt ttc ttg tac gaa ttg gtt aag caa acc 912Lys Ser Lys Ser Gly Tyr Val Phe Leu Tyr Glu Leu Val Lys Gln Thr 290 295 300aga gat cca aag gtc ttg caa gat caa ttg ttg aac att atg gtt gcc 960Arg Asp Pro Lys Val Leu Gln Asp Gln Leu Leu Asn Ile Met Val Ala305 310 315 320gga aga gac acc act gcc ggt ttg ttg tcc ttt gct ttg ttt gaa ttg 1008Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Ala Leu Phe Glu Leu 325 330 335gct aga cac cca gag atg tgg tcc aag ttg aga gaa gaa atc gaa gtt 1056Ala Arg His Pro Glu Met Trp Ser Lys Leu Arg Glu Glu Ile Glu Val 340 345 350aac ttt ggt gtt ggt gaa gac tcc cgc gtt gaa gaa att acc ttc gaa 1104Asn Phe Gly Val Gly Glu Asp Ser Arg Val Glu Glu Ile Thr Phe Glu 355 360 365gcc ttg aag aga tgt gaa tac ttg aag gct atc ctt aac gaa acc ttg 1152Ala Leu Lys Arg Cys Glu Tyr Leu Lys Ala Ile Leu Asn Glu Thr Leu 370 375 380cgt atg tac cca tct gtt cct gtc aac ttt aga acc gcc acc aga gac 1200Arg Met Tyr Pro Ser Val Pro Val Asn Phe Arg Thr Ala Thr Arg Asp385 390 395 400acc act ttg cca aga ggt ggt ggt gct aac ggt acc gac cca atc tac 1248Thr Thr Leu Pro Arg Gly Gly Gly Ala Asn Gly Thr Asp Pro Ile Tyr 405 410 415att cct aaa ggc tcc act gtt gct tac gtt gtc tac aag acc cac cgt 1296Ile Pro Lys Gly Ser Thr Val Ala Tyr Val Val Tyr Lys Thr His Arg 420 425 430ttg gaa gaa tac tac ggt aag gac gct aac gac ttc aga cca gaa aga 1344Leu Glu Glu Tyr Tyr Gly Lys Asp Ala Asn Asp Phe Arg Pro Glu Arg 435 440 445tgg ttt gaa cca tct act aag aag ttg ggc tgg gct tat gtt cca ttc 1392Trp Phe Glu Pro Ser Thr Lys Lys Leu Gly Trp Ala Tyr Val Pro Phe 450 455 460aac ggt ggt cca aga gtc tgc ttg ggt caa caa ttc gcc ttg act gaa 1440Asn Gly Gly Pro Arg Val Cys Leu Gly Gln Gln Phe Ala Leu Thr Glu465 470 475 480gct tct tat gtg atc act aga ttg gcc cag atg ttt gaa act gtc tca 1488Ala Ser Tyr Val Ile Thr Arg Leu Ala Gln Met Phe Glu Thr Val Ser 485 490 495tct gat cca ggt ctc gaa tac cct cca cca aag tgt att cac ttg acc 1536Ser Asp Pro Gly Leu Glu Tyr Pro Pro Pro Lys Cys Ile His Leu Thr 500 505 510atg agt cac aac gat ggt gtc ttt gtc aag atg taa 1572Met Ser His Asn Asp Gly Val Phe Val Lys Met 515 5204523PRTCandida tropicalis 4Met Ala Thr Gln Glu Ile Ile Asp Ser Val Leu Pro Tyr Leu Thr Lys1 5 10 15Trp Tyr Thr Val Ile Thr Ala Ala Val Leu Val Phe Leu Ile Ser Thr 20 25 30Asn Ile Lys Asn Tyr Val Lys Ala Lys Lys Leu Lys Cys Val Asp Pro 35 40 45Pro Tyr Leu Lys Asp Ala Gly Leu Thr Gly Ile Leu Ser Leu Ile Ala 50 55 60Ala Ile Lys Ala Lys Asn Asp Gly Arg Leu Ala Asn Phe Ala Asp Glu65 70 75 80Val Phe Asp Glu Tyr Pro Asn His Thr Phe Tyr Leu Ser Val Ala Gly 85 90 95Ala Leu Lys Ile Val Met Thr Val Asp Pro Glu Asn Ile Lys Ala Val 100 105 110Leu Ala Thr Gln Phe Thr Asp Phe Ser Leu Gly Thr Arg His Ala His 115 120 125Phe Ala Pro Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Glu Gly 130 135 140Trp Lys His Ser Arg Ala Met Leu Arg Pro Gln Phe Ala Arg Asp Gln145 150 155 160Ile Gly His Val Lys Ala Leu Glu Pro His Ile Gln Ile Met Ala Lys 165 170 175Gln Ile Lys Leu Asn Gln Gly Lys Thr Phe Asp Ile Gln Glu Leu Phe 180 185 190Phe Arg Phe Thr Val Asp Thr Ala Thr Glu Phe Leu Phe Gly Glu Ser 195 200 205Val His Ser Leu Tyr Asp Glu Lys Leu Gly Ile Pro Thr Pro Asn Glu 210 215 220Ile Pro Gly Arg Glu Asn Phe Ala Ala Ala Phe Asn Val Ser Gln His225 230 235 240Tyr Leu Ala Thr Arg Ser Tyr Ser Gln Thr

Phe Tyr Phe Leu Thr Asn 245 250 255Pro Lys Glu Phe Arg Asp Cys Asn Ala Lys Val His His Leu Ala Lys 260 265 270Tyr Phe Val Asn Lys Ala Leu Asn Phe Thr Pro Glu Glu Leu Glu Glu 275 280 285Lys Ser Lys Ser Gly Tyr Val Phe Leu Tyr Glu Leu Val Lys Gln Thr 290 295 300Arg Asp Pro Lys Val Leu Gln Asp Gln Leu Leu Asn Ile Met Val Ala305 310 315 320Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Ala Leu Phe Glu Leu 325 330 335Ala Arg His Pro Glu Met Trp Ser Lys Leu Arg Glu Glu Ile Glu Val 340 345 350Asn Phe Gly Val Gly Glu Asp Ser Arg Val Glu Glu Ile Thr Phe Glu 355 360 365Ala Leu Lys Arg Cys Glu Tyr Leu Lys Ala Ile Leu Asn Glu Thr Leu 370 375 380Arg Met Tyr Pro Ser Val Pro Val Asn Phe Arg Thr Ala Thr Arg Asp385 390 395 400Thr Thr Leu Pro Arg Gly Gly Gly Ala Asn Gly Thr Asp Pro Ile Tyr 405 410 415Ile Pro Lys Gly Ser Thr Val Ala Tyr Val Val Tyr Lys Thr His Arg 420 425 430Leu Glu Glu Tyr Tyr Gly Lys Asp Ala Asn Asp Phe Arg Pro Glu Arg 435 440 445Trp Phe Glu Pro Ser Thr Lys Lys Leu Gly Trp Ala Tyr Val Pro Phe 450 455 460Asn Gly Gly Pro Arg Val Cys Leu Gly Gln Gln Phe Ala Leu Thr Glu465 470 475 480Ala Ser Tyr Val Ile Thr Arg Leu Ala Gln Met Phe Glu Thr Val Ser 485 490 495Ser Asp Pro Gly Leu Glu Tyr Pro Pro Pro Lys Cys Ile His Leu Thr 500 505 510Met Ser His Asn Asp Gly Val Phe Val Lys Met 515 52051569DNACandida tropicalisCDS(1)..(1566) 5atg act gta cac gat att atc gcc aca tac ttc acc aaa tgg tac gtg 48Met Thr Val His Asp Ile Ile Ala Thr Tyr Phe Thr Lys Trp Tyr Val1 5 10 15ata gta cca ctc gct ttg att gct tat aga gtc ctc gac tac ttc tat 96Ile Val Pro Leu Ala Leu Ile Ala Tyr Arg Val Leu Asp Tyr Phe Tyr 20 25 30ggc aga tac ttg atg tac aag ctt ggt gct aaa cca ttt ttc cag aaa 144Gly Arg Tyr Leu Met Tyr Lys Leu Gly Ala Lys Pro Phe Phe Gln Lys 35 40 45cag aca gac ggc tgt ttc gga ttc aaa gct ccg ctt gaa ttg ttg aag 192Gln Thr Asp Gly Cys Phe Gly Phe Lys Ala Pro Leu Glu Leu Leu Lys 50 55 60aag aag agc gac ggt acc ctc ata gac ttc aca ctc cag cgt atc cac 240Lys Lys Ser Asp Gly Thr Leu Ile Asp Phe Thr Leu Gln Arg Ile His65 70 75 80gat ctc gat cgt ccc gat atc cca act ttc aca ttc ccg gtc ttt tcc 288Asp Leu Asp Arg Pro Asp Ile Pro Thr Phe Thr Phe Pro Val Phe Ser 85 90 95atc aac ctt gtc aat acc ctt gag ccg gag aac atc aag gcc atc ttg 336Ile Asn Leu Val Asn Thr Leu Glu Pro Glu Asn Ile Lys Ala Ile Leu 100 105 110gcc act cag ttc aac gat ttc tcc ttg ggt acc aga cac tcg cac ttt 384Ala Thr Gln Phe Asn Asp Phe Ser Leu Gly Thr Arg His Ser His Phe 115 120 125gct cct ttg ttg ggt gat ggt atc ttt acg ttg gat ggc gcc ggc tgg 432Ala Pro Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Ala Gly Trp 130 135 140aag cac agc aga tct atg ttg aga cca cag ttt gcc aga gaa cag att 480Lys His Ser Arg Ser Met Leu Arg Pro Gln Phe Ala Arg Glu Gln Ile145 150 155 160tcc cac gtc aag ttg ttg gag cca cac gtt cag gtg ttc ttc aaa cac 528Ser His Val Lys Leu Leu Glu Pro His Val Gln Val Phe Phe Lys His 165 170 175gtc aga aag gca cag ggc aag act ttt gac atc cag gaa ttg ttt ttc 576Val Arg Lys Ala Gln Gly Lys Thr Phe Asp Ile Gln Glu Leu Phe Phe 180 185 190aga ttg acc gtc gac tcc gcc acc gag ttt ttg ttt ggt gaa tcc gtt 624Arg Leu Thr Val Asp Ser Ala Thr Glu Phe Leu Phe Gly Glu Ser Val 195 200 205gag tcc ttg aga gat gaa tct atc ggc atg tcc atc aat gcg ctt gac 672Glu Ser Leu Arg Asp Glu Ser Ile Gly Met Ser Ile Asn Ala Leu Asp 210 215 220ttt gac ggc aag gct ggc ttt gct gat gct ttt aac tat tcg cag aat 720Phe Asp Gly Lys Ala Gly Phe Ala Asp Ala Phe Asn Tyr Ser Gln Asn225 230 235 240tat ttg gct tcg aga gcg gtt atg caa caa ttg tac tgg gtg ttg aac 768Tyr Leu Ala Ser Arg Ala Val Met Gln Gln Leu Tyr Trp Val Leu Asn 245 250 255ggg aaa aag ttt aag gag tgc aac gct aaa gtg cac aag ttt gct gac 816Gly Lys Lys Phe Lys Glu Cys Asn Ala Lys Val His Lys Phe Ala Asp 260 265 270tac tac gtc aac aag gct ttg gac ttg acg cct gaa caa ttg gaa aag 864Tyr Tyr Val Asn Lys Ala Leu Asp Leu Thr Pro Glu Gln Leu Glu Lys 275 280 285cag gat ggt tat gtg ttt ttg tac gaa ttg gtc aag caa acc aga gac 912Gln Asp Gly Tyr Val Phe Leu Tyr Glu Leu Val Lys Gln Thr Arg Asp 290 295 300aag caa gtg ttg aga gac caa ttg ttg aac atc atg gtt gct ggt aga 960Lys Gln Val Leu Arg Asp Gln Leu Leu Asn Ile Met Val Ala Gly Arg305 310 315 320gac acc acc gcc ggt ttg ttg tcg ttt gtt ttc ttt gaa ttg gcc aga 1008Asp Thr Thr Ala Gly Leu Leu Ser Phe Val Phe Phe Glu Leu Ala Arg 325 330 335aac cca gaa gtt acc aac aag ttg aga gaa gaa att gag gac aag ttt 1056Asn Pro Glu Val Thr Asn Lys Leu Arg Glu Glu Ile Glu Asp Lys Phe 340 345 350gga ctc ggt gag aat gct agt gtt gaa gac att tcc ttt gag tcg ttg 1104Gly Leu Gly Glu Asn Ala Ser Val Glu Asp Ile Ser Phe Glu Ser Leu 355 360 365aag tcc tgt gaa tac ttg aag gct gtt ctc aac gaa acc ttg aga ttg 1152Lys Ser Cys Glu Tyr Leu Lys Ala Val Leu Asn Glu Thr Leu Arg Leu 370 375 380tac cca tcc gtg cca cag aat ttc aga gtt gcc acc aag aac act acc 1200Tyr Pro Ser Val Pro Gln Asn Phe Arg Val Ala Thr Lys Asn Thr Thr385 390 395 400ctc cca aga ggt ggt ggt aag gac ggg ttg tct cct gtt ttg gtg aga 1248Leu Pro Arg Gly Gly Gly Lys Asp Gly Leu Ser Pro Val Leu Val Arg 405 410 415aag ggt cag acc gtt att tac ggt gtc tac gca gcc cac aga aac cca 1296Lys Gly Gln Thr Val Ile Tyr Gly Val Tyr Ala Ala His Arg Asn Pro 420 425 430gct gtt tac ggt aag gac gct ctt gag ttt aga cca gag aga tgg ttt 1344Ala Val Tyr Gly Lys Asp Ala Leu Glu Phe Arg Pro Glu Arg Trp Phe 435 440 445gag cca gag aca aag aag ctt ggc tgg gcc ttc ctc cca ttc aac ggt 1392Glu Pro Glu Thr Lys Lys Leu Gly Trp Ala Phe Leu Pro Phe Asn Gly 450 455 460ggt cca aga atc tgt ttg gga cag cag ttt gcc ttg aca gaa gct tcg 1440Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe Ala Leu Thr Glu Ala Ser465 470 475 480tat gtc act gtc agg ttg ctc cag gag ttt gca cac ttg tct atg gac 1488Tyr Val Thr Val Arg Leu Leu Gln Glu Phe Ala His Leu Ser Met Asp 485 490 495cca gac acc gaa tat cca cct aag aaa atg tcg cat ttg acc atg tcg 1536Pro Asp Thr Glu Tyr Pro Pro Lys Lys Met Ser His Leu Thr Met Ser 500 505 510ctt ttc gac ggt gcc aat att gag atg tat tag 1569Leu Phe Asp Gly Ala Asn Ile Glu Met Tyr 515 5206522PRTCandida tropicalis 6Met Thr Val His Asp Ile Ile Ala Thr Tyr Phe Thr Lys Trp Tyr Val1 5 10 15Ile Val Pro Leu Ala Leu Ile Ala Tyr Arg Val Leu Asp Tyr Phe Tyr 20 25 30Gly Arg Tyr Leu Met Tyr Lys Leu Gly Ala Lys Pro Phe Phe Gln Lys 35 40 45Gln Thr Asp Gly Cys Phe Gly Phe Lys Ala Pro Leu Glu Leu Leu Lys 50 55 60Lys Lys Ser Asp Gly Thr Leu Ile Asp Phe Thr Leu Gln Arg Ile His65 70 75 80Asp Leu Asp Arg Pro Asp Ile Pro Thr Phe Thr Phe Pro Val Phe Ser 85 90 95Ile Asn Leu Val Asn Thr Leu Glu Pro Glu Asn Ile Lys Ala Ile Leu 100 105 110Ala Thr Gln Phe Asn Asp Phe Ser Leu Gly Thr Arg His Ser His Phe 115 120 125Ala Pro Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Ala Gly Trp 130 135 140Lys His Ser Arg Ser Met Leu Arg Pro Gln Phe Ala Arg Glu Gln Ile145 150 155 160Ser His Val Lys Leu Leu Glu Pro His Val Gln Val Phe Phe Lys His 165 170 175Val Arg Lys Ala Gln Gly Lys Thr Phe Asp Ile Gln Glu Leu Phe Phe 180 185 190Arg Leu Thr Val Asp Ser Ala Thr Glu Phe Leu Phe Gly Glu Ser Val 195 200 205Glu Ser Leu Arg Asp Glu Ser Ile Gly Met Ser Ile Asn Ala Leu Asp 210 215 220Phe Asp Gly Lys Ala Gly Phe Ala Asp Ala Phe Asn Tyr Ser Gln Asn225 230 235 240Tyr Leu Ala Ser Arg Ala Val Met Gln Gln Leu Tyr Trp Val Leu Asn 245 250 255Gly Lys Lys Phe Lys Glu Cys Asn Ala Lys Val His Lys Phe Ala Asp 260 265 270Tyr Tyr Val Asn Lys Ala Leu Asp Leu Thr Pro Glu Gln Leu Glu Lys 275 280 285Gln Asp Gly Tyr Val Phe Leu Tyr Glu Leu Val Lys Gln Thr Arg Asp 290 295 300Lys Gln Val Leu Arg Asp Gln Leu Leu Asn Ile Met Val Ala Gly Arg305 310 315 320Asp Thr Thr Ala Gly Leu Leu Ser Phe Val Phe Phe Glu Leu Ala Arg 325 330 335Asn Pro Glu Val Thr Asn Lys Leu Arg Glu Glu Ile Glu Asp Lys Phe 340 345 350Gly Leu Gly Glu Asn Ala Ser Val Glu Asp Ile Ser Phe Glu Ser Leu 355 360 365Lys Ser Cys Glu Tyr Leu Lys Ala Val Leu Asn Glu Thr Leu Arg Leu 370 375 380Tyr Pro Ser Val Pro Gln Asn Phe Arg Val Ala Thr Lys Asn Thr Thr385 390 395 400Leu Pro Arg Gly Gly Gly Lys Asp Gly Leu Ser Pro Val Leu Val Arg 405 410 415Lys Gly Gln Thr Val Ile Tyr Gly Val Tyr Ala Ala His Arg Asn Pro 420 425 430Ala Val Tyr Gly Lys Asp Ala Leu Glu Phe Arg Pro Glu Arg Trp Phe 435 440 445Glu Pro Glu Thr Lys Lys Leu Gly Trp Ala Phe Leu Pro Phe Asn Gly 450 455 460Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe Ala Leu Thr Glu Ala Ser465 470 475 480Tyr Val Thr Val Arg Leu Leu Gln Glu Phe Ala His Leu Ser Met Asp 485 490 495Pro Asp Thr Glu Tyr Pro Pro Lys Lys Met Ser His Leu Thr Met Ser 500 505 510Leu Phe Asp Gly Ala Asn Ile Glu Met Tyr 515 52071569DNACandida tropicalisCDS(1)..(1566) 7atg act gca cag gat att atc gcc aca tac atc acc aaa tgg tac gtg 48Met Thr Ala Gln Asp Ile Ile Ala Thr Tyr Ile Thr Lys Trp Tyr Val1 5 10 15ata gta cca ctc gct ttg att gct tat agg gtc ctc gac tac ttt tac 96Ile Val Pro Leu Ala Leu Ile Ala Tyr Arg Val Leu Asp Tyr Phe Tyr 20 25 30ggc aga tac ttg atg tac aag ctt ggt gct aaa ccg ttt ttc cag aaa 144Gly Arg Tyr Leu Met Tyr Lys Leu Gly Ala Lys Pro Phe Phe Gln Lys 35 40 45caa aca gac ggt tat ttc gga ttc aaa gct cca ctt gaa ttg tta aaa 192Gln Thr Asp Gly Tyr Phe Gly Phe Lys Ala Pro Leu Glu Leu Leu Lys 50 55 60aag aag agt gac ggt acc ctc ata gac ttc act ctc gag cgt atc caa 240Lys Lys Ser Asp Gly Thr Leu Ile Asp Phe Thr Leu Glu Arg Ile Gln65 70 75 80gcg ctc aat cgt cca gat atc cca act ttt aca ttc cca atc ttt tcc 288Ala Leu Asn Arg Pro Asp Ile Pro Thr Phe Thr Phe Pro Ile Phe Ser 85 90 95atc aac ctt atc agc acc ctt gag ccg gag aac atc aag gct atc ttg 336Ile Asn Leu Ile Ser Thr Leu Glu Pro Glu Asn Ile Lys Ala Ile Leu 100 105 110gcc acc cag ttc aac gat ttc tcc ttg ggc acc aga cac tcg cac ttt 384Ala Thr Gln Phe Asn Asp Phe Ser Leu Gly Thr Arg His Ser His Phe 115 120 125gct cct ttg ttg ggc gat ggt atc ttt acc ttg gac ggt gcc ggc tgg 432Ala Pro Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Ala Gly Trp 130 135 140aag cac agc aga tct atg ttg aga cca cag ttt gcc aga gaa cag att 480Lys His Ser Arg Ser Met Leu Arg Pro Gln Phe Ala Arg Glu Gln Ile145 150 155 160tcc cac gtc aag ttg ttg gag cca cac atg cag gtg ttc ttc aag cac 528Ser His Val Lys Leu Leu Glu Pro His Met Gln Val Phe Phe Lys His 165 170 175gtc aga aag gca cag ggc aag act ttt gac atc caa gaa ttg ttt ttc 576Val Arg Lys Ala Gln Gly Lys Thr Phe Asp Ile Gln Glu Leu Phe Phe 180 185 190aga ttg acc gtc gac tcc gcc act gag ttt ttg ttt ggt gaa tcc gtt 624Arg Leu Thr Val Asp Ser Ala Thr Glu Phe Leu Phe Gly Glu Ser Val 195 200 205gag tcc ttg aga gat gaa tct att ggg atg tcc atc aat gca ctt gac 672Glu Ser Leu Arg Asp Glu Ser Ile Gly Met Ser Ile Asn Ala Leu Asp 210 215 220ttt gac ggc aag gct ggc ttt gct gat gct ttt aac tac tcg cag aac 720Phe Asp Gly Lys Ala Gly Phe Ala Asp Ala Phe Asn Tyr Ser Gln Asn225 230 235 240tat ttg gct tcg aga gcg gtt atg caa caa ttg tac tgg gtg ttg aac 768Tyr Leu Ala Ser Arg Ala Val Met Gln Gln Leu Tyr Trp Val Leu Asn 245 250 255ggg aaa aag ttt aag gag tgc aac gct aaa gtg cac aag ttt gct gac 816Gly Lys Lys Phe Lys Glu Cys Asn Ala Lys Val His Lys Phe Ala Asp 260 265 270tat tac gtc agc aag gct ttg gac ttg aca cct gaa caa ttg gaa aag 864Tyr Tyr Val Ser Lys Ala Leu Asp Leu Thr Pro Glu Gln Leu Glu Lys 275 280 285cag gat ggt tat gtg ttc ttg tac gag ttg gtc aag caa acc aga gac 912Gln Asp Gly Tyr Val Phe Leu Tyr Glu Leu Val Lys Gln Thr Arg Asp 290 295 300agg caa gtg ttg aga gac cag ttg ttg aac atc atg gtt gcc ggt aga 960Arg Gln Val Leu Arg Asp Gln Leu Leu Asn Ile Met Val Ala Gly Arg305 310 315 320gac acc acc gcc ggt ttg ttg tcg ttt gtt ttc ttt gaa ttg gcc aga 1008Asp Thr Thr Ala Gly Leu Leu Ser Phe Val Phe Phe Glu Leu Ala Arg 325 330 335aac cca gag gtg acc aac aag ttg aga gaa gaa atc gag gac aag ttt 1056Asn Pro Glu Val Thr Asn Lys Leu Arg Glu Glu Ile Glu Asp Lys Phe 340 345 350ggt ctt ggt gag aat gct cgt gtt gaa gac att tcc ttt gag tcg ttg 1104Gly Leu Gly Glu Asn Ala Arg Val Glu Asp Ile Ser Phe Glu Ser Leu 355 360 365aag tca tgt gaa tac ttg aag gct gtt ctc aac gaa act ttg aga ttg 1152Lys Ser Cys Glu Tyr Leu Lys Ala Val Leu Asn Glu Thr Leu Arg Leu 370 375 380tac cca tcc gtg cca cag aat ttc aga gtt gcc acc aaa aac act acc 1200Tyr Pro Ser Val Pro Gln Asn Phe Arg Val Ala Thr Lys Asn Thr Thr385 390 395 400ctt cca agg gga ggt ggt aag gac ggg tta tct cct gtt ttg gtc aga 1248Leu Pro Arg Gly Gly Gly Lys Asp Gly Leu Ser Pro Val Leu Val Arg 405 410 415aag ggt caa acc gtt atg tac ggt gtc tac gct gcc cac aga aac cca 1296Lys Gly Gln Thr Val Met Tyr Gly Val Tyr Ala Ala His Arg Asn Pro 420 425 430gct gtc tac ggt aag gac gcc ctt gag ttt aga cca gag agg tgg ttt 1344Ala Val Tyr Gly Lys Asp Ala Leu Glu Phe Arg Pro Glu Arg Trp Phe 435 440 445gag cca gag aca aag aag ctt ggc tgg gcc ttc ctt cca ttc aac ggt 1392Glu Pro Glu Thr Lys Lys Leu Gly Trp Ala Phe Leu Pro Phe Asn Gly 450 455 460ggt cca aga att tgc ttg gga cag cag ttt gcc ttg aca gaa gct tcg 1440Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe Ala Leu Thr Glu Ala Ser465 470 475 480tat gtc act gtc aga ttg ctc caa gag ttt gga cac ttg tct atg gac 1488Tyr Val Thr Val Arg Leu Leu Gln Glu Phe Gly His Leu Ser Met Asp 485 490 495ccc aac acc gaa tat cca cct agg aaa atg tcg cat ttg acc atg tcc 1536Pro Asn Thr Glu Tyr Pro Pro Arg Lys Met Ser His Leu Thr Met Ser 500

505 510ctt ttc gac ggt gcc aac att gag atg tat tag 1569Leu Phe Asp Gly Ala Asn Ile Glu Met Tyr 515 5208522PRTCandida tropicalis 8Met Thr Ala Gln Asp Ile Ile Ala Thr Tyr Ile Thr Lys Trp Tyr Val1 5 10 15Ile Val Pro Leu Ala Leu Ile Ala Tyr Arg Val Leu Asp Tyr Phe Tyr 20 25 30Gly Arg Tyr Leu Met Tyr Lys Leu Gly Ala Lys Pro Phe Phe Gln Lys 35 40 45Gln Thr Asp Gly Tyr Phe Gly Phe Lys Ala Pro Leu Glu Leu Leu Lys 50 55 60Lys Lys Ser Asp Gly Thr Leu Ile Asp Phe Thr Leu Glu Arg Ile Gln65 70 75 80Ala Leu Asn Arg Pro Asp Ile Pro Thr Phe Thr Phe Pro Ile Phe Ser 85 90 95Ile Asn Leu Ile Ser Thr Leu Glu Pro Glu Asn Ile Lys Ala Ile Leu 100 105 110Ala Thr Gln Phe Asn Asp Phe Ser Leu Gly Thr Arg His Ser His Phe 115 120 125Ala Pro Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Ala Gly Trp 130 135 140Lys His Ser Arg Ser Met Leu Arg Pro Gln Phe Ala Arg Glu Gln Ile145 150 155 160Ser His Val Lys Leu Leu Glu Pro His Met Gln Val Phe Phe Lys His 165 170 175Val Arg Lys Ala Gln Gly Lys Thr Phe Asp Ile Gln Glu Leu Phe Phe 180 185 190Arg Leu Thr Val Asp Ser Ala Thr Glu Phe Leu Phe Gly Glu Ser Val 195 200 205Glu Ser Leu Arg Asp Glu Ser Ile Gly Met Ser Ile Asn Ala Leu Asp 210 215 220Phe Asp Gly Lys Ala Gly Phe Ala Asp Ala Phe Asn Tyr Ser Gln Asn225 230 235 240Tyr Leu Ala Ser Arg Ala Val Met Gln Gln Leu Tyr Trp Val Leu Asn 245 250 255Gly Lys Lys Phe Lys Glu Cys Asn Ala Lys Val His Lys Phe Ala Asp 260 265 270Tyr Tyr Val Ser Lys Ala Leu Asp Leu Thr Pro Glu Gln Leu Glu Lys 275 280 285Gln Asp Gly Tyr Val Phe Leu Tyr Glu Leu Val Lys Gln Thr Arg Asp 290 295 300Arg Gln Val Leu Arg Asp Gln Leu Leu Asn Ile Met Val Ala Gly Arg305 310 315 320Asp Thr Thr Ala Gly Leu Leu Ser Phe Val Phe Phe Glu Leu Ala Arg 325 330 335Asn Pro Glu Val Thr Asn Lys Leu Arg Glu Glu Ile Glu Asp Lys Phe 340 345 350Gly Leu Gly Glu Asn Ala Arg Val Glu Asp Ile Ser Phe Glu Ser Leu 355 360 365Lys Ser Cys Glu Tyr Leu Lys Ala Val Leu Asn Glu Thr Leu Arg Leu 370 375 380Tyr Pro Ser Val Pro Gln Asn Phe Arg Val Ala Thr Lys Asn Thr Thr385 390 395 400Leu Pro Arg Gly Gly Gly Lys Asp Gly Leu Ser Pro Val Leu Val Arg 405 410 415Lys Gly Gln Thr Val Met Tyr Gly Val Tyr Ala Ala His Arg Asn Pro 420 425 430Ala Val Tyr Gly Lys Asp Ala Leu Glu Phe Arg Pro Glu Arg Trp Phe 435 440 445Glu Pro Glu Thr Lys Lys Leu Gly Trp Ala Phe Leu Pro Phe Asn Gly 450 455 460Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe Ala Leu Thr Glu Ala Ser465 470 475 480Tyr Val Thr Val Arg Leu Leu Gln Glu Phe Gly His Leu Ser Met Asp 485 490 495Pro Asn Thr Glu Tyr Pro Pro Arg Lys Met Ser His Leu Thr Met Ser 500 505 510Leu Phe Asp Gly Ala Asn Ile Glu Met Tyr 515 52091623DNACandida tropicalisCDS(1)..(1620) 9atg tcg tct tct cca tcg ttt gcc caa gag gtt ctc gct acc act agt 48Met Ser Ser Ser Pro Ser Phe Ala Gln Glu Val Leu Ala Thr Thr Ser1 5 10 15cct tac atc gag tac ttt ctt gac aac tac acc aga tgg tac tac ttc 96Pro Tyr Ile Glu Tyr Phe Leu Asp Asn Tyr Thr Arg Trp Tyr Tyr Phe 20 25 30ata cct ttg gtg ctt ctt tcg ttg aac ttt ata agt ttg ctc cac aca 144Ile Pro Leu Val Leu Leu Ser Leu Asn Phe Ile Ser Leu Leu His Thr 35 40 45agg tac ttg gaa cgc agg ttc cac gcc aag cca ctc ggt aac ttt gtc 192Arg Tyr Leu Glu Arg Arg Phe His Ala Lys Pro Leu Gly Asn Phe Val 50 55 60agg gac cct acg ttt ggt atc gct act ccg ttg ctt ttg atc tac ttg 240Arg Asp Pro Thr Phe Gly Ile Ala Thr Pro Leu Leu Leu Ile Tyr Leu65 70 75 80aag tcg aaa ggt acg gtc atg aag ttt gct tgg ggc ctc tgg aac aac 288Lys Ser Lys Gly Thr Val Met Lys Phe Ala Trp Gly Leu Trp Asn Asn 85 90 95aag tac atc gtc aga gac cca aag tac aag aca act ggg ctc agg att 336Lys Tyr Ile Val Arg Asp Pro Lys Tyr Lys Thr Thr Gly Leu Arg Ile 100 105 110gtt ggc ctc cca ttg att gaa acc atg gac cca gag aac atc aag gct 384Val Gly Leu Pro Leu Ile Glu Thr Met Asp Pro Glu Asn Ile Lys Ala 115 120 125gtt ttg gct act cag ttc aat gat ttc tct ttg gga acc aga cac gat 432Val Leu Ala Thr Gln Phe Asn Asp Phe Ser Leu Gly Thr Arg His Asp 130 135 140ttc ttg tac tcc ttg ttg ggt gac ggt att ttc acc ttg gac ggt gct 480Phe Leu Tyr Ser Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Ala145 150 155 160ggc tgg aaa cat agt aga act atg ttg aga cca cag ttt gct aga gaa 528Gly Trp Lys His Ser Arg Thr Met Leu Arg Pro Gln Phe Ala Arg Glu 165 170 175cag gtt tct cac gtc aag ttg ttg gag cca cac gtt cag gtg ttc ttc 576Gln Val Ser His Val Lys Leu Leu Glu Pro His Val Gln Val Phe Phe 180 185 190aag cac gtt aga aag cac cgc ggt caa acg ttc gac atc caa gaa ttg 624Lys His Val Arg Lys His Arg Gly Gln Thr Phe Asp Ile Gln Glu Leu 195 200 205ttc ttc agg ttg acc gtc gac tcc gcc acc gag ttc ttg ttt ggt gag 672Phe Phe Arg Leu Thr Val Asp Ser Ala Thr Glu Phe Leu Phe Gly Glu 210 215 220tct gct gaa tcc ttg agg gac gaa tct att gga ttg acc cca acc acc 720Ser Ala Glu Ser Leu Arg Asp Glu Ser Ile Gly Leu Thr Pro Thr Thr225 230 235 240aag gat ttc gat ggc aga aga gat ttc gct gac gct ttc aac tat tcg 768Lys Asp Phe Asp Gly Arg Arg Asp Phe Ala Asp Ala Phe Asn Tyr Ser 245 250 255cag act tac cag gcc tac aga ttt ttg ttg caa caa atg tac tgg atc 816Gln Thr Tyr Gln Ala Tyr Arg Phe Leu Leu Gln Gln Met Tyr Trp Ile 260 265 270ttg aat ggc tcg gaa ttc aga aag tcg att gct gtc gtg cac aag ttt 864Leu Asn Gly Ser Glu Phe Arg Lys Ser Ile Ala Val Val His Lys Phe 275 280 285gct gac cac tat gtg caa aag gct ttg gag ttg acc gac gat gac ttg 912Ala Asp His Tyr Val Gln Lys Ala Leu Glu Leu Thr Asp Asp Asp Leu 290 295 300cag aaa caa gac ggc tat gtg ttc ttg tac gag ttg gct aag caa acc 960Gln Lys Gln Asp Gly Tyr Val Phe Leu Tyr Glu Leu Ala Lys Gln Thr305 310 315 320aga gac cca aag gtc ttg aga gac cag tta ttg aac att ttg gtt gcc 1008Arg Asp Pro Lys Val Leu Arg Asp Gln Leu Leu Asn Ile Leu Val Ala 325 330 335ggt aga gac acg acc gcc ggt ttg ttg tca ttt gtt ttc tac gag ttg 1056Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Val Phe Tyr Glu Leu 340 345 350tca aga aac cct gag gtg ttt gct aag ttg aga gag gag gtg gaa aac 1104Ser Arg Asn Pro Glu Val Phe Ala Lys Leu Arg Glu Glu Val Glu Asn 355 360 365aga ttt gga ctc ggt gaa gaa gct cgt gtt gaa gag atc tcg ttt gag 1152Arg Phe Gly Leu Gly Glu Glu Ala Arg Val Glu Glu Ile Ser Phe Glu 370 375 380tcc ttg aag tct tgt gag tac ttg aag gct gtc atc aat gaa acc ttg 1200Ser Leu Lys Ser Cys Glu Tyr Leu Lys Ala Val Ile Asn Glu Thr Leu385 390 395 400aga ttg tac cca tcg gtt cca cac aac ttt aga gtt gct acc aga aac 1248Arg Leu Tyr Pro Ser Val Pro His Asn Phe Arg Val Ala Thr Arg Asn 405 410 415act acc ctc cca aga ggt ggt ggt gaa gat gga tac tcg cca att gtc 1296Thr Thr Leu Pro Arg Gly Gly Gly Glu Asp Gly Tyr Ser Pro Ile Val 420 425 430gtc aag aag ggt caa gtt gtc atg tac act gtt att gct acc cac aga 1344Val Lys Lys Gly Gln Val Val Met Tyr Thr Val Ile Ala Thr His Arg 435 440 445gac cca agt atc tac ggt gcc gac gct gac gtc ttc aga cca gaa aga 1392Asp Pro Ser Ile Tyr Gly Ala Asp Ala Asp Val Phe Arg Pro Glu Arg 450 455 460tgg ttt gaa cca gaa act aga aag ttg ggc tgg gca tac gtt cca ttc 1440Trp Phe Glu Pro Glu Thr Arg Lys Leu Gly Trp Ala Tyr Val Pro Phe465 470 475 480aat ggt ggt cca aga atc tgt ttg ggt caa cag ttt gcc ttg acc gaa 1488Asn Gly Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe Ala Leu Thr Glu 485 490 495gct tca tac gtc act gtc aga ttg ctc cag gag ttt gca cac ttg tct 1536Ala Ser Tyr Val Thr Val Arg Leu Leu Gln Glu Phe Ala His Leu Ser 500 505 510atg gac cca gac acc gaa tat cca cca aaa ttg cag aac acc ttg acc 1584Met Asp Pro Asp Thr Glu Tyr Pro Pro Lys Leu Gln Asn Thr Leu Thr 515 520 525ttg tcg ctc ttt gat ggt gct gat gtt aga atg tac taa 1623Leu Ser Leu Phe Asp Gly Ala Asp Val Arg Met Tyr 530 535 54010540PRTCandida tropicalis 10Met Ser Ser Ser Pro Ser Phe Ala Gln Glu Val Leu Ala Thr Thr Ser1 5 10 15Pro Tyr Ile Glu Tyr Phe Leu Asp Asn Tyr Thr Arg Trp Tyr Tyr Phe 20 25 30Ile Pro Leu Val Leu Leu Ser Leu Asn Phe Ile Ser Leu Leu His Thr 35 40 45Arg Tyr Leu Glu Arg Arg Phe His Ala Lys Pro Leu Gly Asn Phe Val 50 55 60Arg Asp Pro Thr Phe Gly Ile Ala Thr Pro Leu Leu Leu Ile Tyr Leu65 70 75 80Lys Ser Lys Gly Thr Val Met Lys Phe Ala Trp Gly Leu Trp Asn Asn 85 90 95Lys Tyr Ile Val Arg Asp Pro Lys Tyr Lys Thr Thr Gly Leu Arg Ile 100 105 110Val Gly Leu Pro Leu Ile Glu Thr Met Asp Pro Glu Asn Ile Lys Ala 115 120 125Val Leu Ala Thr Gln Phe Asn Asp Phe Ser Leu Gly Thr Arg His Asp 130 135 140Phe Leu Tyr Ser Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Ala145 150 155 160Gly Trp Lys His Ser Arg Thr Met Leu Arg Pro Gln Phe Ala Arg Glu 165 170 175Gln Val Ser His Val Lys Leu Leu Glu Pro His Val Gln Val Phe Phe 180 185 190Lys His Val Arg Lys His Arg Gly Gln Thr Phe Asp Ile Gln Glu Leu 195 200 205Phe Phe Arg Leu Thr Val Asp Ser Ala Thr Glu Phe Leu Phe Gly Glu 210 215 220Ser Ala Glu Ser Leu Arg Asp Glu Ser Ile Gly Leu Thr Pro Thr Thr225 230 235 240Lys Asp Phe Asp Gly Arg Arg Asp Phe Ala Asp Ala Phe Asn Tyr Ser 245 250 255Gln Thr Tyr Gln Ala Tyr Arg Phe Leu Leu Gln Gln Met Tyr Trp Ile 260 265 270Leu Asn Gly Ser Glu Phe Arg Lys Ser Ile Ala Val Val His Lys Phe 275 280 285Ala Asp His Tyr Val Gln Lys Ala Leu Glu Leu Thr Asp Asp Asp Leu 290 295 300Gln Lys Gln Asp Gly Tyr Val Phe Leu Tyr Glu Leu Ala Lys Gln Thr305 310 315 320Arg Asp Pro Lys Val Leu Arg Asp Gln Leu Leu Asn Ile Leu Val Ala 325 330 335Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Val Phe Tyr Glu Leu 340 345 350Ser Arg Asn Pro Glu Val Phe Ala Lys Leu Arg Glu Glu Val Glu Asn 355 360 365Arg Phe Gly Leu Gly Glu Glu Ala Arg Val Glu Glu Ile Ser Phe Glu 370 375 380Ser Leu Lys Ser Cys Glu Tyr Leu Lys Ala Val Ile Asn Glu Thr Leu385 390 395 400Arg Leu Tyr Pro Ser Val Pro His Asn Phe Arg Val Ala Thr Arg Asn 405 410 415Thr Thr Leu Pro Arg Gly Gly Gly Glu Asp Gly Tyr Ser Pro Ile Val 420 425 430Val Lys Lys Gly Gln Val Val Met Tyr Thr Val Ile Ala Thr His Arg 435 440 445Asp Pro Ser Ile Tyr Gly Ala Asp Ala Asp Val Phe Arg Pro Glu Arg 450 455 460Trp Phe Glu Pro Glu Thr Arg Lys Leu Gly Trp Ala Tyr Val Pro Phe465 470 475 480Asn Gly Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe Ala Leu Thr Glu 485 490 495Ala Ser Tyr Val Thr Val Arg Leu Leu Gln Glu Phe Ala His Leu Ser 500 505 510Met Asp Pro Asp Thr Glu Tyr Pro Pro Lys Leu Gln Asn Thr Leu Thr 515 520 525Leu Ser Leu Phe Asp Gly Ala Asp Val Arg Met Tyr 530 535 540111623DNACandida tropicalisCDS(1)..(1620) 11atg tcg tct tct cca tcg ttt gct cag gag gtt ctc gct acc act agt 48Met Ser Ser Ser Pro Ser Phe Ala Gln Glu Val Leu Ala Thr Thr Ser1 5 10 15cct tac atc gag tac ttt ctt gac aac tac acc aga tgg tac tac ttc 96Pro Tyr Ile Glu Tyr Phe Leu Asp Asn Tyr Thr Arg Trp Tyr Tyr Phe 20 25 30atc cct ttg gtg ctt ctt tcg ttg aac ttc atc agc ttg ctc cac aca 144Ile Pro Leu Val Leu Leu Ser Leu Asn Phe Ile Ser Leu Leu His Thr 35 40 45aag tac ttg gaa cgc agg ttc cac gcc aag ccg ctc ggt aac gtc gtg 192Lys Tyr Leu Glu Arg Arg Phe His Ala Lys Pro Leu Gly Asn Val Val 50 55 60ttg gat cct acg ttt ggt atc gct act ccg ttg atc ttg atc tac tta 240Leu Asp Pro Thr Phe Gly Ile Ala Thr Pro Leu Ile Leu Ile Tyr Leu65 70 75 80aag tcg aaa ggt aca gtc atg aag ttt gcc tgg agc ttc tgg aac aac 288Lys Ser Lys Gly Thr Val Met Lys Phe Ala Trp Ser Phe Trp Asn Asn 85 90 95aag tac att gtc aaa gac cca aag tac aag acc act ggc ctt aga att 336Lys Tyr Ile Val Lys Asp Pro Lys Tyr Lys Thr Thr Gly Leu Arg Ile 100 105 110gtc ggc ctc cca ttg att gaa acc ata gac cca gag aac atc aaa gct 384Val Gly Leu Pro Leu Ile Glu Thr Ile Asp Pro Glu Asn Ile Lys Ala 115 120 125gtg ttg gct act cag ttc aac gat ttc tcc ttg gga act aga cac gat 432Val Leu Ala Thr Gln Phe Asn Asp Phe Ser Leu Gly Thr Arg His Asp 130 135 140ttc ttg tac tcc ttg ttg ggc gat ggt att ttt acc ttg gac ggt gct 480Phe Leu Tyr Ser Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Ala145 150 155 160ggc tgg aaa cac agt aga act atg ttg aga cca cag ttt gct aga gaa 528Gly Trp Lys His Ser Arg Thr Met Leu Arg Pro Gln Phe Ala Arg Glu 165 170 175cag gtt tcc cac gtc aag ttg ttg gaa cca cac gtt cag gtg ttc ttc 576Gln Val Ser His Val Lys Leu Leu Glu Pro His Val Gln Val Phe Phe 180 185 190aag cac gtt aga aaa cac cgc ggt cag act ttt gac atc caa gaa ttg 624Lys His Val Arg Lys His Arg Gly Gln Thr Phe Asp Ile Gln Glu Leu 195 200 205ttc ttc aga ttg acc gtc gac tcc gcc acc gag ttc ttg ttt ggt gag 672Phe Phe Arg Leu Thr Val Asp Ser Ala Thr Glu Phe Leu Phe Gly Glu 210 215 220tct gct gaa tcc ttg aga gac gac tct gtt ggt ttg acc cca acc acc 720Ser Ala Glu Ser Leu Arg Asp Asp Ser Val Gly Leu Thr Pro Thr Thr225 230 235 240aag gat ttc gaa ggc aga gga gat ttc gct gac gct ttc aac tac tcg 768Lys Asp Phe Glu Gly Arg Gly Asp Phe Ala Asp Ala Phe Asn Tyr Ser 245 250 255cag act tac cag gcc tac aga ttt ttg ttg caa caa atg tac tgg att 816Gln Thr Tyr Gln Ala Tyr Arg Phe Leu Leu Gln Gln Met Tyr Trp Ile 260 265 270ttg aat ggc gcg gaa ttc aga aag tcg att gcc atc gtg cac aag ttt 864Leu Asn Gly Ala Glu Phe Arg Lys Ser Ile Ala Ile Val His Lys Phe 275 280 285gct gac cac tat gtg caa aag gct ttg gag ttg acc gac gat gac ttg 912Ala Asp His Tyr Val Gln Lys Ala Leu Glu Leu Thr Asp Asp Asp Leu 290 295 300cag aaa caa gac ggc tat gtg ttc ttg tac gag ttg gct aag caa act 960Gln Lys Gln Asp Gly Tyr Val Phe Leu Tyr Glu Leu Ala Lys Gln Thr305

310 315 320aga gac cca aag gtc ttg aga gac cag ttg ttg aac att ttg gtt gcc 1008Arg Asp Pro Lys Val Leu Arg Asp Gln Leu Leu Asn Ile Leu Val Ala 325 330 335ggt aga gac acg acc gcc ggt ttg ttg tcg ttt gtg ttc tac gag ttg 1056Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Val Phe Tyr Glu Leu 340 345 350tcg aga aac cct gaa gtg ttt gcc aag ttg aga gag gag gtg gaa aac 1104Ser Arg Asn Pro Glu Val Phe Ala Lys Leu Arg Glu Glu Val Glu Asn 355 360 365aga ttt gga ctc ggc gaa gag gct cgt gtt gaa gag atc tct ttt gag 1152Arg Phe Gly Leu Gly Glu Glu Ala Arg Val Glu Glu Ile Ser Phe Glu 370 375 380tcc ttg aag tcc tgt gag tac ttg aag gct gtc atc aat gaa gcc ttg 1200Ser Leu Lys Ser Cys Glu Tyr Leu Lys Ala Val Ile Asn Glu Ala Leu385 390 395 400aga ttg tac cca tct gtt cca cac aac ttc aga gtt gcc acc aga aac 1248Arg Leu Tyr Pro Ser Val Pro His Asn Phe Arg Val Ala Thr Arg Asn 405 410 415act acc ctt cca aga ggc ggt ggt aaa gac gga tgc tcg cca att gtt 1296Thr Thr Leu Pro Arg Gly Gly Gly Lys Asp Gly Cys Ser Pro Ile Val 420 425 430gtc aag aag ggt caa gtt gtc atg tac act gtc att ggt acc cac aga 1344Val Lys Lys Gly Gln Val Val Met Tyr Thr Val Ile Gly Thr His Arg 435 440 445gac cca agt atc tac ggt gcc gac gcc gac gtc ttc aga cca gaa aga 1392Asp Pro Ser Ile Tyr Gly Ala Asp Ala Asp Val Phe Arg Pro Glu Arg 450 455 460tgg ttc gag cca gaa act aga aag ttg ggc tgg gca tat gtt cca ttc 1440Trp Phe Glu Pro Glu Thr Arg Lys Leu Gly Trp Ala Tyr Val Pro Phe465 470 475 480aat ggt ggt cca aga atc tgt ttg ggt cag cag ttt gcc ttg act gaa 1488Asn Gly Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe Ala Leu Thr Glu 485 490 495gct tca tac gtc act gtc aga ttg ctc caa gag ttt gga aac ttg tcc 1536Ala Ser Tyr Val Thr Val Arg Leu Leu Gln Glu Phe Gly Asn Leu Ser 500 505 510ctg gat cca aac gct gag tac cca cca aaa ttg cag aac acc ttg acc 1584Leu Asp Pro Asn Ala Glu Tyr Pro Pro Lys Leu Gln Asn Thr Leu Thr 515 520 525ttg tca ctc ttt gat ggt gct gac gtt aga atg ttc taa 1623Leu Ser Leu Phe Asp Gly Ala Asp Val Arg Met Phe 530 535 54012540PRTCandida tropicalis 12Met Ser Ser Ser Pro Ser Phe Ala Gln Glu Val Leu Ala Thr Thr Ser1 5 10 15Pro Tyr Ile Glu Tyr Phe Leu Asp Asn Tyr Thr Arg Trp Tyr Tyr Phe 20 25 30Ile Pro Leu Val Leu Leu Ser Leu Asn Phe Ile Ser Leu Leu His Thr 35 40 45Lys Tyr Leu Glu Arg Arg Phe His Ala Lys Pro Leu Gly Asn Val Val 50 55 60Leu Asp Pro Thr Phe Gly Ile Ala Thr Pro Leu Ile Leu Ile Tyr Leu65 70 75 80Lys Ser Lys Gly Thr Val Met Lys Phe Ala Trp Ser Phe Trp Asn Asn 85 90 95Lys Tyr Ile Val Lys Asp Pro Lys Tyr Lys Thr Thr Gly Leu Arg Ile 100 105 110Val Gly Leu Pro Leu Ile Glu Thr Ile Asp Pro Glu Asn Ile Lys Ala 115 120 125Val Leu Ala Thr Gln Phe Asn Asp Phe Ser Leu Gly Thr Arg His Asp 130 135 140Phe Leu Tyr Ser Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Ala145 150 155 160Gly Trp Lys His Ser Arg Thr Met Leu Arg Pro Gln Phe Ala Arg Glu 165 170 175Gln Val Ser His Val Lys Leu Leu Glu Pro His Val Gln Val Phe Phe 180 185 190Lys His Val Arg Lys His Arg Gly Gln Thr Phe Asp Ile Gln Glu Leu 195 200 205Phe Phe Arg Leu Thr Val Asp Ser Ala Thr Glu Phe Leu Phe Gly Glu 210 215 220Ser Ala Glu Ser Leu Arg Asp Asp Ser Val Gly Leu Thr Pro Thr Thr225 230 235 240Lys Asp Phe Glu Gly Arg Gly Asp Phe Ala Asp Ala Phe Asn Tyr Ser 245 250 255Gln Thr Tyr Gln Ala Tyr Arg Phe Leu Leu Gln Gln Met Tyr Trp Ile 260 265 270Leu Asn Gly Ala Glu Phe Arg Lys Ser Ile Ala Ile Val His Lys Phe 275 280 285Ala Asp His Tyr Val Gln Lys Ala Leu Glu Leu Thr Asp Asp Asp Leu 290 295 300Gln Lys Gln Asp Gly Tyr Val Phe Leu Tyr Glu Leu Ala Lys Gln Thr305 310 315 320Arg Asp Pro Lys Val Leu Arg Asp Gln Leu Leu Asn Ile Leu Val Ala 325 330 335Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Val Phe Tyr Glu Leu 340 345 350Ser Arg Asn Pro Glu Val Phe Ala Lys Leu Arg Glu Glu Val Glu Asn 355 360 365Arg Phe Gly Leu Gly Glu Glu Ala Arg Val Glu Glu Ile Ser Phe Glu 370 375 380Ser Leu Lys Ser Cys Glu Tyr Leu Lys Ala Val Ile Asn Glu Ala Leu385 390 395 400Arg Leu Tyr Pro Ser Val Pro His Asn Phe Arg Val Ala Thr Arg Asn 405 410 415Thr Thr Leu Pro Arg Gly Gly Gly Lys Asp Gly Cys Ser Pro Ile Val 420 425 430Val Lys Lys Gly Gln Val Val Met Tyr Thr Val Ile Gly Thr His Arg 435 440 445Asp Pro Ser Ile Tyr Gly Ala Asp Ala Asp Val Phe Arg Pro Glu Arg 450 455 460Trp Phe Glu Pro Glu Thr Arg Lys Leu Gly Trp Ala Tyr Val Pro Phe465 470 475 480Asn Gly Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe Ala Leu Thr Glu 485 490 495Ala Ser Tyr Val Thr Val Arg Leu Leu Gln Glu Phe Gly Asn Leu Ser 500 505 510Leu Asp Pro Asn Ala Glu Tyr Pro Pro Lys Leu Gln Asn Thr Leu Thr 515 520 525Leu Ser Leu Phe Asp Gly Ala Asp Val Arg Met Phe 530 535 540131554DNACandida tropicalisCDS(1)..(1551) 13atg att gaa caa ctc cta gaa tat tgg tat gtc gtt gtg cca gtg ttg 48Met Ile Glu Gln Leu Leu Glu Tyr Trp Tyr Val Val Val Pro Val Leu1 5 10 15tac atc atc aaa caa ctc ctt gca tac aca aag act cgc gtc ttg atg 96Tyr Ile Ile Lys Gln Leu Leu Ala Tyr Thr Lys Thr Arg Val Leu Met 20 25 30aaa aag ttg ggt gct gct cca gtc aca aac aag ttg tac gac aac gct 144Lys Lys Leu Gly Ala Ala Pro Val Thr Asn Lys Leu Tyr Asp Asn Ala 35 40 45ttc ggt atc gtc aat gga tgg aag gct ctc cag ttc aag aaa gag ggc 192Phe Gly Ile Val Asn Gly Trp Lys Ala Leu Gln Phe Lys Lys Glu Gly 50 55 60agg gct caa gag tac aac gat tac aag ttt gac cac tcc aag aac cca 240Arg Ala Gln Glu Tyr Asn Asp Tyr Lys Phe Asp His Ser Lys Asn Pro65 70 75 80agc gtg ggc acc tac gtc agt att ctt ttc ggc acc agg atc gtc gtg 288Ser Val Gly Thr Tyr Val Ser Ile Leu Phe Gly Thr Arg Ile Val Val 85 90 95acc aaa gat cca gag aat atc aaa gct att ttg gca acc cag ttt ggt 336Thr Lys Asp Pro Glu Asn Ile Lys Ala Ile Leu Ala Thr Gln Phe Gly 100 105 110gat ttt tct ttg ggc aag agg cac act ctt ttt aag cct ttg tta ggt 384Asp Phe Ser Leu Gly Lys Arg His Thr Leu Phe Lys Pro Leu Leu Gly 115 120 125gat ggg atc ttc aca ttg gac ggc gaa ggc tgg aag cac agc aga gcc 432Asp Gly Ile Phe Thr Leu Asp Gly Glu Gly Trp Lys His Ser Arg Ala 130 135 140atg ttg aga cca cag ttt gcc aga gaa caa gtt gct cat gtg acg tcg 480Met Leu Arg Pro Gln Phe Ala Arg Glu Gln Val Ala His Val Thr Ser145 150 155 160ttg gaa cca cac ttc cag ttg ttg aag aag cat att ctt aag cac aag 528Leu Glu Pro His Phe Gln Leu Leu Lys Lys His Ile Leu Lys His Lys 165 170 175ggt gaa tac ttt gat atc cag gaa ttg ttc ttt aga ttt acc gtt gat 576Gly Glu Tyr Phe Asp Ile Gln Glu Leu Phe Phe Arg Phe Thr Val Asp 180 185 190tcg gcc acg gag ttc tta ttt ggt gag tcc gtg cac tcc tta aag gac 624Ser Ala Thr Glu Phe Leu Phe Gly Glu Ser Val His Ser Leu Lys Asp 195 200 205gaa tct att ggt atc aac caa gac gat ata gat ttt gct ggt aga aag 672Glu Ser Ile Gly Ile Asn Gln Asp Asp Ile Asp Phe Ala Gly Arg Lys 210 215 220gac ttt gct gag tcg ttc aac aaa gcc cag gaa tac ttg gct att aga 720Asp Phe Ala Glu Ser Phe Asn Lys Ala Gln Glu Tyr Leu Ala Ile Arg225 230 235 240acc ttg gtg cag acg ttc tac tgg ttg gtc aac aac aag gag ttt aga 768Thr Leu Val Gln Thr Phe Tyr Trp Leu Val Asn Asn Lys Glu Phe Arg 245 250 255gac tgt acc aag ctg gtg cac aag ttc acc aac tac tat gtt cag aaa 816Asp Cys Thr Lys Leu Val His Lys Phe Thr Asn Tyr Tyr Val Gln Lys 260 265 270gct ttg gat gct agc cca gaa gag ctt gaa aag caa agt ggg tat gtg 864Ala Leu Asp Ala Ser Pro Glu Glu Leu Glu Lys Gln Ser Gly Tyr Val 275 280 285ttc ttg tac gag ctt gtc aag cag aca aga gac ccc aat gtg ttg cgt 912Phe Leu Tyr Glu Leu Val Lys Gln Thr Arg Asp Pro Asn Val Leu Arg 290 295 300gac cag tct ttg aac atc ttg ttg gcc gga aga gac acc act gct ggg 960Asp Gln Ser Leu Asn Ile Leu Leu Ala Gly Arg Asp Thr Thr Ala Gly305 310 315 320ttg ttg tcg ttt gct gtc ttt gag ttg gcc aga cac cca gag atc tgg 1008Leu Leu Ser Phe Ala Val Phe Glu Leu Ala Arg His Pro Glu Ile Trp 325 330 335gcc aag ttg aga gag gaa att gaa caa cag ttt ggt ctt gga gaa gac 1056Ala Lys Leu Arg Glu Glu Ile Glu Gln Gln Phe Gly Leu Gly Glu Asp 340 345 350tct cgt gtt gaa gag att acc ttt gag agc ttg aag aga tgt gag tac 1104Ser Arg Val Glu Glu Ile Thr Phe Glu Ser Leu Lys Arg Cys Glu Tyr 355 360 365ttg aaa gcg ttc ctt aat gaa acc ttg cgt att tac cca agt gtc cca 1152Leu Lys Ala Phe Leu Asn Glu Thr Leu Arg Ile Tyr Pro Ser Val Pro 370 375 380aga aac ttc aga atc gcc acc aag aac acg aca ttg cca agg ggc ggt 1200Arg Asn Phe Arg Ile Ala Thr Lys Asn Thr Thr Leu Pro Arg Gly Gly385 390 395 400ggt tca gac ggt acc tcg cca atc ttg atc caa aag gga gaa gct gtg 1248Gly Ser Asp Gly Thr Ser Pro Ile Leu Ile Gln Lys Gly Glu Ala Val 405 410 415tcg tat ggt atc aac tct act cat ttg gac cct gtc tat tac ggc cct 1296Ser Tyr Gly Ile Asn Ser Thr His Leu Asp Pro Val Tyr Tyr Gly Pro 420 425 430gat gct gct gag ttc aga cca gag aga tgg ttt gag cca tca acc aaa 1344Asp Ala Ala Glu Phe Arg Pro Glu Arg Trp Phe Glu Pro Ser Thr Lys 435 440 445aag ctc ggc tgg gct tac ttg cca ttc aac ggt ggt cca aga atc tgt 1392Lys Leu Gly Trp Ala Tyr Leu Pro Phe Asn Gly Gly Pro Arg Ile Cys 450 455 460ttg ggt cag cag ttt gcc ttg acg gaa gct ggc tat gtg ttg gtt aga 1440Leu Gly Gln Gln Phe Ala Leu Thr Glu Ala Gly Tyr Val Leu Val Arg465 470 475 480ttg gtg caa gag ttc tcc cac gtt agg ctg gac cca gac gag gtg tac 1488Leu Val Gln Glu Phe Ser His Val Arg Leu Asp Pro Asp Glu Val Tyr 485 490 495ccg cca aag agg ttg acc aac ttg acc atg tgt ttg cag gat ggt gct 1536Pro Pro Lys Arg Leu Thr Asn Leu Thr Met Cys Leu Gln Asp Gly Ala 500 505 510att gtc aag ttt gac tag 1554Ile Val Lys Phe Asp 51514517PRTCandida tropicalis 14Met Ile Glu Gln Leu Leu Glu Tyr Trp Tyr Val Val Val Pro Val Leu1 5 10 15Tyr Ile Ile Lys Gln Leu Leu Ala Tyr Thr Lys Thr Arg Val Leu Met 20 25 30Lys Lys Leu Gly Ala Ala Pro Val Thr Asn Lys Leu Tyr Asp Asn Ala 35 40 45Phe Gly Ile Val Asn Gly Trp Lys Ala Leu Gln Phe Lys Lys Glu Gly 50 55 60Arg Ala Gln Glu Tyr Asn Asp Tyr Lys Phe Asp His Ser Lys Asn Pro65 70 75 80Ser Val Gly Thr Tyr Val Ser Ile Leu Phe Gly Thr Arg Ile Val Val 85 90 95Thr Lys Asp Pro Glu Asn Ile Lys Ala Ile Leu Ala Thr Gln Phe Gly 100 105 110Asp Phe Ser Leu Gly Lys Arg His Thr Leu Phe Lys Pro Leu Leu Gly 115 120 125Asp Gly Ile Phe Thr Leu Asp Gly Glu Gly Trp Lys His Ser Arg Ala 130 135 140Met Leu Arg Pro Gln Phe Ala Arg Glu Gln Val Ala His Val Thr Ser145 150 155 160Leu Glu Pro His Phe Gln Leu Leu Lys Lys His Ile Leu Lys His Lys 165 170 175Gly Glu Tyr Phe Asp Ile Gln Glu Leu Phe Phe Arg Phe Thr Val Asp 180 185 190Ser Ala Thr Glu Phe Leu Phe Gly Glu Ser Val His Ser Leu Lys Asp 195 200 205Glu Ser Ile Gly Ile Asn Gln Asp Asp Ile Asp Phe Ala Gly Arg Lys 210 215 220Asp Phe Ala Glu Ser Phe Asn Lys Ala Gln Glu Tyr Leu Ala Ile Arg225 230 235 240Thr Leu Val Gln Thr Phe Tyr Trp Leu Val Asn Asn Lys Glu Phe Arg 245 250 255Asp Cys Thr Lys Leu Val His Lys Phe Thr Asn Tyr Tyr Val Gln Lys 260 265 270Ala Leu Asp Ala Ser Pro Glu Glu Leu Glu Lys Gln Ser Gly Tyr Val 275 280 285Phe Leu Tyr Glu Leu Val Lys Gln Thr Arg Asp Pro Asn Val Leu Arg 290 295 300Asp Gln Ser Leu Asn Ile Leu Leu Ala Gly Arg Asp Thr Thr Ala Gly305 310 315 320Leu Leu Ser Phe Ala Val Phe Glu Leu Ala Arg His Pro Glu Ile Trp 325 330 335Ala Lys Leu Arg Glu Glu Ile Glu Gln Gln Phe Gly Leu Gly Glu Asp 340 345 350Ser Arg Val Glu Glu Ile Thr Phe Glu Ser Leu Lys Arg Cys Glu Tyr 355 360 365Leu Lys Ala Phe Leu Asn Glu Thr Leu Arg Ile Tyr Pro Ser Val Pro 370 375 380Arg Asn Phe Arg Ile Ala Thr Lys Asn Thr Thr Leu Pro Arg Gly Gly385 390 395 400Gly Ser Asp Gly Thr Ser Pro Ile Leu Ile Gln Lys Gly Glu Ala Val 405 410 415Ser Tyr Gly Ile Asn Ser Thr His Leu Asp Pro Val Tyr Tyr Gly Pro 420 425 430Asp Ala Ala Glu Phe Arg Pro Glu Arg Trp Phe Glu Pro Ser Thr Lys 435 440 445Lys Leu Gly Trp Ala Tyr Leu Pro Phe Asn Gly Gly Pro Arg Ile Cys 450 455 460Leu Gly Gln Gln Phe Ala Leu Thr Glu Ala Gly Tyr Val Leu Val Arg465 470 475 480Leu Val Gln Glu Phe Ser His Val Arg Leu Asp Pro Asp Glu Val Tyr 485 490 495Pro Pro Lys Arg Leu Thr Asn Leu Thr Met Cys Leu Gln Asp Gly Ala 500 505 510Ile Val Lys Phe Asp 515151554DNACandida tropicalisCDS(1)..(1551) 15atg att gaa caa atc cta gaa tat tgg tat att gtt gtg cct gtg ttg 48Met Ile Glu Gln Ile Leu Glu Tyr Trp Tyr Ile Val Val Pro Val Leu1 5 10 15tac atc atc aaa caa ctc att gcc tac agc aag act cgc gtc ttg atg 96Tyr Ile Ile Lys Gln Leu Ile Ala Tyr Ser Lys Thr Arg Val Leu Met 20 25 30aaa cag ttg ggt gct gct cca atc aca aac cag ttg tac gac aac gtt 144Lys Gln Leu Gly Ala Ala Pro Ile Thr Asn Gln Leu Tyr Asp Asn Val 35 40 45ttc ggt atc gtc aac gga tgg aag gct ctc cag ttc aag aaa gag ggc 192Phe Gly Ile Val Asn Gly Trp Lys Ala Leu Gln Phe Lys Lys Glu Gly 50 55 60aga gct caa gag tac aac gat cac aag ttt gac agc tcc aag aac cca 240Arg Ala Gln Glu Tyr Asn Asp His Lys Phe Asp Ser Ser Lys Asn Pro 65 70 75 80agc gtc ggc acc tat gtc agt att ctt ttt ggc acc aag att gtc gtg 288Ser Val Gly Thr Tyr Val Ser Ile Leu Phe Gly Thr Lys Ile Val Val85 90 95acc aag gat cca gag aat atc aaa gct att ttg gca acc cag ttt ggc 336Thr Lys Asp Pro Glu Asn Ile Lys Ala Ile Leu Ala Thr Gln Phe Gly 100 105 110gat ttt tct ttg ggc aag aga cac gct ctt ttt aaa cct ttg tta ggt 384Asp Phe Ser Leu Gly Lys Arg His Ala Leu Phe Lys Pro Leu Leu Gly

115 120 125gat ggg atc ttc acc ttg gac ggc gaa ggc tgg aag cat agc aga tcc 432Asp Gly Ile Phe Thr Leu Asp Gly Glu Gly Trp Lys His Ser Arg Ser 130 135 140atg tta aga cca cag ttt gcc aga gaa caa gtt gct cat gtg acg tcg 480Met Leu Arg Pro Gln Phe Ala Arg Glu Gln Val Ala His Val Thr Ser 145 150 155 160ttg gaa cca cac ttc cag ttg ttg aag aag cat atc ctt aaa cac aag 528Leu Glu Pro His Phe Gln Leu Leu Lys Lys His Ile Leu Lys His Lys165 170 175ggt gag tac ttt gat atc cag gaa ttg ttc ttt aga ttt act gtc gac 576Gly Glu Tyr Phe Asp Ile Gln Glu Leu Phe Phe Arg Phe Thr Val Asp 180 185 190tcg gcc acg gag ttc tta ttt ggt gag tcc gtg cac tcc tta aag gac 624Ser Ala Thr Glu Phe Leu Phe Gly Glu Ser Val His Ser Leu Lys Asp 195 200 205gaa act atc ggt atc aac caa gac gat ata gat ttt gct ggt aga aag 672Glu Thr Ile Gly Ile Asn Gln Asp Asp Ile Asp Phe Ala Gly Arg Lys 210 215 220gac ttt gct gag tcg ttc aac aaa gcc cag gag tat ttg tct att aga 720Asp Phe Ala Glu Ser Phe Asn Lys Ala Gln Glu Tyr Leu Ser Ile Arg 225 230 235 240att ttg gtg cag acc ttc tac tgg ttg atc aac aac aag gag ttt aga 768Ile Leu Val Gln Thr Phe Tyr Trp Leu Ile Asn Asn Lys Glu Phe Arg245 250 255gac tgt acc aag ctg gtg cac aag ttt acc aac tac tat gtt cag aaa 816Asp Cys Thr Lys Leu Val His Lys Phe Thr Asn Tyr Tyr Val Gln Lys 260 265 270gct ttg gat gct acc cca gag gaa ctt gaa aag caa ggc ggg tat gtg 864Ala Leu Asp Ala Thr Pro Glu Glu Leu Glu Lys Gln Gly Gly Tyr Val 275 280 285ttc ttg tat gag ctt gtc aag cag acg aga gac ccc aag gtg ttg cgt 912Phe Leu Tyr Glu Leu Val Lys Gln Thr Arg Asp Pro Lys Val Leu Arg 290 295 300gac cag tct ttg aac atc ttg ttg gca gga aga gac acc act gct ggg 960Asp Gln Ser Leu Asn Ile Leu Leu Ala Gly Arg Asp Thr Thr Ala Gly 305 310 315 320ttg ttg tcc ttt gct gtg ttt gag ttg gcc aga aac cca cac atc tgg 1008Leu Leu Ser Phe Ala Val Phe Glu Leu Ala Arg Asn Pro His Ile Trp325 330 335gcc aag ttg aga gag gaa att gaa cag cag ttt ggt ctt gga gaa gac 1056Ala Lys Leu Arg Glu Glu Ile Glu Gln Gln Phe Gly Leu Gly Glu Asp 340 345 350tct cgt gtt gaa gag att acc ttt gag agc ttg aag aga tgt gag tac 1104Ser Arg Val Glu Glu Ile Thr Phe Glu Ser Leu Lys Arg Cys Glu Tyr 355 360 365ttg aaa gcg ttc ctt aac gaa acc ttg cgt gtt tac cca agt gtc cca 1152Leu Lys Ala Phe Leu Asn Glu Thr Leu Arg Val Tyr Pro Ser Val Pro 370 375 380aga aac ttc aga atc gcc acc aag aat aca aca ttg cca agg ggt ggt 1200Arg Asn Phe Arg Ile Ala Thr Lys Asn Thr Thr Leu Pro Arg Gly Gly 385 390 395 400ggt cca gac ggt acc cag cca atc ttg atc caa aag gga gaa ggt gtg 1248Gly Pro Asp Gly Thr Gln Pro Ile Leu Ile Gln Lys Gly Glu Gly Val405 410 415tcg tat ggt atc aac tct acc cac tta gat cct gtc tat tat ggc cct 1296Ser Tyr Gly Ile Asn Ser Thr His Leu Asp Pro Val Tyr Tyr Gly Pro 420 425 430gat gct gct gag ttc aga cca gag aga tgg ttt gag cca tca acc aga 1344Asp Ala Ala Glu Phe Arg Pro Glu Arg Trp Phe Glu Pro Ser Thr Arg 435 440 445aag ctc ggc tgg gct tac ttg cca ttc aac ggt ggg cca cga atc tgt 1392Lys Leu Gly Trp Ala Tyr Leu Pro Phe Asn Gly Gly Pro Arg Ile Cys 450 455 460ttg ggt cag cag ttt gcc ttg acc gaa gct ggt tac gtt ttg gtc aga 1440Leu Gly Gln Gln Phe Ala Leu Thr Glu Ala Gly Tyr Val Leu Val Arg 465 470 475 480ttg gtg caa gag ttc tcc cac att agg ctg gac cca gat gaa gtg tat 1488Leu Val Gln Glu Phe Ser His Ile Arg Leu Asp Pro Asp Glu Val Tyr485 490 495cca cca aag agg ttg acc aac ttg acc atg tgt ttg cag gat ggt gct 1536Pro Pro Lys Arg Leu Thr Asn Leu Thr Met Cys Leu Gln Asp Gly Ala 500 505 510att gtc aag ttt gac tag 1554Ile Val Lys Phe Asp 51516517PRTCandida tropicalis 16Met Ile Glu Gln Ile Leu Glu Tyr Trp Tyr Ile Val Val Pro Val Leu1 5 10 15Tyr Ile Ile Lys Gln Leu Ile Ala Tyr Ser Lys Thr Arg Val Leu Met 20 25 30Lys Gln Leu Gly Ala Ala Pro Ile Thr Asn Gln Leu Tyr Asp Asn Val 35 40 45Phe Gly Ile Val Asn Gly Trp Lys Ala Leu Gln Phe Lys Lys Glu Gly 50 55 60Arg Ala Gln Glu Tyr Asn Asp His Lys Phe Asp Ser Ser Lys Asn Pro65 70 75 80Ser Val Gly Thr Tyr Val Ser Ile Leu Phe Gly Thr Lys Ile Val Val 85 90 95Thr Lys Asp Pro Glu Asn Ile Lys Ala Ile Leu Ala Thr Gln Phe Gly 100 105 110Asp Phe Ser Leu Gly Lys Arg His Ala Leu Phe Lys Pro Leu Leu Gly 115 120 125Asp Gly Ile Phe Thr Leu Asp Gly Glu Gly Trp Lys His Ser Arg Ser 130 135 140Met Leu Arg Pro Gln Phe Ala Arg Glu Gln Val Ala His Val Thr Ser145 150 155 160Leu Glu Pro His Phe Gln Leu Leu Lys Lys His Ile Leu Lys His Lys 165 170 175Gly Glu Tyr Phe Asp Ile Gln Glu Leu Phe Phe Arg Phe Thr Val Asp 180 185 190Ser Ala Thr Glu Phe Leu Phe Gly Glu Ser Val His Ser Leu Lys Asp 195 200 205Glu Thr Ile Gly Ile Asn Gln Asp Asp Ile Asp Phe Ala Gly Arg Lys 210 215 220Asp Phe Ala Glu Ser Phe Asn Lys Ala Gln Glu Tyr Leu Ser Ile Arg225 230 235 240Ile Leu Val Gln Thr Phe Tyr Trp Leu Ile Asn Asn Lys Glu Phe Arg 245 250 255Asp Cys Thr Lys Leu Val His Lys Phe Thr Asn Tyr Tyr Val Gln Lys 260 265 270Ala Leu Asp Ala Thr Pro Glu Glu Leu Glu Lys Gln Gly Gly Tyr Val 275 280 285Phe Leu Tyr Glu Leu Val Lys Gln Thr Arg Asp Pro Lys Val Leu Arg 290 295 300Asp Gln Ser Leu Asn Ile Leu Leu Ala Gly Arg Asp Thr Thr Ala Gly305 310 315 320Leu Leu Ser Phe Ala Val Phe Glu Leu Ala Arg Asn Pro His Ile Trp 325 330 335Ala Lys Leu Arg Glu Glu Ile Glu Gln Gln Phe Gly Leu Gly Glu Asp 340 345 350Ser Arg Val Glu Glu Ile Thr Phe Glu Ser Leu Lys Arg Cys Glu Tyr 355 360 365Leu Lys Ala Phe Leu Asn Glu Thr Leu Arg Val Tyr Pro Ser Val Pro 370 375 380Arg Asn Phe Arg Ile Ala Thr Lys Asn Thr Thr Leu Pro Arg Gly Gly385 390 395 400Gly Pro Asp Gly Thr Gln Pro Ile Leu Ile Gln Lys Gly Glu Gly Val 405 410 415Ser Tyr Gly Ile Asn Ser Thr His Leu Asp Pro Val Tyr Tyr Gly Pro 420 425 430Asp Ala Ala Glu Phe Arg Pro Glu Arg Trp Phe Glu Pro Ser Thr Arg 435 440 445Lys Leu Gly Trp Ala Tyr Leu Pro Phe Asn Gly Gly Pro Arg Ile Cys 450 455 460Leu Gly Gln Gln Phe Ala Leu Thr Glu Ala Gly Tyr Val Leu Val Arg465 470 475 480Leu Val Gln Glu Phe Ser His Ile Arg Leu Asp Pro Asp Glu Val Tyr 485 490 495Pro Pro Lys Arg Leu Thr Asn Leu Thr Met Cys Leu Gln Asp Gly Ala 500 505 510Ile Val Lys Phe Asp 515171539DNACandida tropicalisCDS(1)..(1536) 17atg ctc gat cag atc tta cat tac tgg tac att gtc ttg cca ttg ttg 48Met Leu Asp Gln Ile Leu His Tyr Trp Tyr Ile Val Leu Pro Leu Leu1 5 10 15gcc att atc aac cag atc gtg gct cat gtc agg acc aat tat ttg atg 96Ala Ile Ile Asn Gln Ile Val Ala His Val Arg Thr Asn Tyr Leu Met 20 25 30aag aaa ttg ggt gct aag cca ttc aca cac gtc caa cgt gac ggg tgg 144Lys Lys Leu Gly Ala Lys Pro Phe Thr His Val Gln Arg Asp Gly Trp 35 40 45ttg ggc ttc aaa ttc ggc cgt gaa ttc ctc aaa gca aaa agt gct ggg 192Leu Gly Phe Lys Phe Gly Arg Glu Phe Leu Lys Ala Lys Ser Ala Gly 50 55 60aga ctg gtt gat tta atc atc tcc cgt ttc cac gat aat gag gac act 240Arg Leu Val Asp Leu Ile Ile Ser Arg Phe His Asp Asn Glu Asp Thr65 70 75 80ttc tcc agc tat gct ttt ggc aac cat gtg gtg ttc acc agg gac ccc 288Phe Ser Ser Tyr Ala Phe Gly Asn His Val Val Phe Thr Arg Asp Pro 85 90 95gag aat atc aag gcg ctt ttg gca acc cag ttt ggt gat ttt tca ttg 336Glu Asn Ile Lys Ala Leu Leu Ala Thr Gln Phe Gly Asp Phe Ser Leu 100 105 110ggc agc agg gtc aag ttc ttc aaa cca tta ttg ggg tac ggt atc ttc 384Gly Ser Arg Val Lys Phe Phe Lys Pro Leu Leu Gly Tyr Gly Ile Phe 115 120 125aca ttg gac gcc gaa ggc tgg aag cac agc aga gcc atg ttg aga cca 432Thr Leu Asp Ala Glu Gly Trp Lys His Ser Arg Ala Met Leu Arg Pro 130 135 140cag ttt gcc aga gaa caa gtt gct cat gtg acg tcg ttg gaa cca cac 480Gln Phe Ala Arg Glu Gln Val Ala His Val Thr Ser Leu Glu Pro His145 150 155 160ttc cag ttg ttg aag aag cat atc ctt aaa cac aag ggt gag tac ttt 528Phe Gln Leu Leu Lys Lys His Ile Leu Lys His Lys Gly Glu Tyr Phe 165 170 175gat atc cag gaa ttg ttc ttt aga ttt act gtc gac tcg gcc acg gag 576Asp Ile Gln Glu Leu Phe Phe Arg Phe Thr Val Asp Ser Ala Thr Glu 180 185 190ttc tta ttt ggt gag tcc gtg cac tcc tta aag gac gag gaa att ggc 624Phe Leu Phe Gly Glu Ser Val His Ser Leu Lys Asp Glu Glu Ile Gly 195 200 205tac gac acg aaa gac atg tct gaa gaa aga cgc aga ttt gcc gac gcg 672Tyr Asp Thr Lys Asp Met Ser Glu Glu Arg Arg Arg Phe Ala Asp Ala 210 215 220ttc aac aag tcg caa gtc tac gtg gcc acc aga gtt gct tta cag aac 720Phe Asn Lys Ser Gln Val Tyr Val Ala Thr Arg Val Ala Leu Gln Asn225 230 235 240ttg tac tgg ttg gtc aac aac aaa gag ttc aag gag tgc aat gac att 768Leu Tyr Trp Leu Val Asn Asn Lys Glu Phe Lys Glu Cys Asn Asp Ile 245 250 255gtc cac aag ttt acc aac tac tat gtt cag aaa gcc ttg gat gct acc 816Val His Lys Phe Thr Asn Tyr Tyr Val Gln Lys Ala Leu Asp Ala Thr 260 265 270cca gag gaa ctt gaa aag caa ggc ggg tat gtg ttc ttg tat gag ctt 864Pro Glu Glu Leu Glu Lys Gln Gly Gly Tyr Val Phe Leu Tyr Glu Leu 275 280 285gtc aag cag acg aga gac ccc aag gtg ttg cgt gac cag tct ttg aac 912Val Lys Gln Thr Arg Asp Pro Lys Val Leu Arg Asp Gln Ser Leu Asn 290 295 300atc ttg ttg gca gga aga gac acc act gct ggg ttg ttg tcc ttt gct 960Ile Leu Leu Ala Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Ala305 310 315 320gtg ttt gag ttg gcc aga aac cca cac atc tgg gcc aag ttg aga gag 1008Val Phe Glu Leu Ala Arg Asn Pro His Ile Trp Ala Lys Leu Arg Glu 325 330 335gaa att gaa cag cag ttt ggt ctt gga gaa gac tct cgt gtt gaa gag 1056Glu Ile Glu Gln Gln Phe Gly Leu Gly Glu Asp Ser Arg Val Glu Glu 340 345 350att acc ttt gag agc ttg aag aga tgt gag tac ttg aag gcc gtg ttg 1104Ile Thr Phe Glu Ser Leu Lys Arg Cys Glu Tyr Leu Lys Ala Val Leu 355 360 365aac gaa act ttg aga tta cac cca agt gtc cca aga aac gca aga ttt 1152Asn Glu Thr Leu Arg Leu His Pro Ser Val Pro Arg Asn Ala Arg Phe 370 375 380gcg att aaa gac acg act tta cca aga ggc ggt ggc ccc aac ggc aag 1200Ala Ile Lys Asp Thr Thr Leu Pro Arg Gly Gly Gly Pro Asn Gly Lys385 390 395 400gat cct atc ttg atc agg aag gat gag gtg gtg cag tac tcc atc tcg 1248Asp Pro Ile Leu Ile Arg Lys Asp Glu Val Val Gln Tyr Ser Ile Ser 405 410 415gca act cag aca aat cct gct tat tat ggc gcc gat gct gct gat ttt 1296Ala Thr Gln Thr Asn Pro Ala Tyr Tyr Gly Ala Asp Ala Ala Asp Phe 420 425 430aga ccg gaa aga tgg ttt gaa cca tca act aga aac ttg gga tgg gct 1344Arg Pro Glu Arg Trp Phe Glu Pro Ser Thr Arg Asn Leu Gly Trp Ala 435 440 445ttc ttg cca ttc aac ggt ggt cca aga atc tgt ttg gga caa cag ttt 1392Phe Leu Pro Phe Asn Gly Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe 450 455 460gct ttg act gaa gcc ggt tac gtt ttg gtt aga ctt gtt cag gag ttt 1440Ala Leu Thr Glu Ala Gly Tyr Val Leu Val Arg Leu Val Gln Glu Phe465 470 475 480cca aac ttg tca caa gac ccc gaa acc aag tac cca cca cct aga ttg 1488Pro Asn Leu Ser Gln Asp Pro Glu Thr Lys Tyr Pro Pro Pro Arg Leu 485 490 495gca cac ttg acg atg tgc ttg ttt gac ggt gca cac gtc aag atg tca 1536Ala His Leu Thr Met Cys Leu Phe Asp Gly Ala His Val Lys Met Ser 500 505 510tag 153918512PRTCandida tropicalis 18Met Leu Asp Gln Ile Leu His Tyr Trp Tyr Ile Val Leu Pro Leu Leu1 5 10 15Ala Ile Ile Asn Gln Ile Val Ala His Val Arg Thr Asn Tyr Leu Met 20 25 30Lys Lys Leu Gly Ala Lys Pro Phe Thr His Val Gln Arg Asp Gly Trp 35 40 45Leu Gly Phe Lys Phe Gly Arg Glu Phe Leu Lys Ala Lys Ser Ala Gly 50 55 60Arg Leu Val Asp Leu Ile Ile Ser Arg Phe His Asp Asn Glu Asp Thr65 70 75 80Phe Ser Ser Tyr Ala Phe Gly Asn His Val Val Phe Thr Arg Asp Pro 85 90 95Glu Asn Ile Lys Ala Leu Leu Ala Thr Gln Phe Gly Asp Phe Ser Leu 100 105 110Gly Ser Arg Val Lys Phe Phe Lys Pro Leu Leu Gly Tyr Gly Ile Phe 115 120 125Thr Leu Asp Ala Glu Gly Trp Lys His Ser Arg Ala Met Leu Arg Pro 130 135 140Gln Phe Ala Arg Glu Gln Val Ala His Val Thr Ser Leu Glu Pro His145 150 155 160Phe Gln Leu Leu Lys Lys His Ile Leu Lys His Lys Gly Glu Tyr Phe 165 170 175Asp Ile Gln Glu Leu Phe Phe Arg Phe Thr Val Asp Ser Ala Thr Glu 180 185 190Phe Leu Phe Gly Glu Ser Val His Ser Leu Lys Asp Glu Glu Ile Gly 195 200 205Tyr Asp Thr Lys Asp Met Ser Glu Glu Arg Arg Arg Phe Ala Asp Ala 210 215 220Phe Asn Lys Ser Gln Val Tyr Val Ala Thr Arg Val Ala Leu Gln Asn225 230 235 240Leu Tyr Trp Leu Val Asn Asn Lys Glu Phe Lys Glu Cys Asn Asp Ile 245 250 255Val His Lys Phe Thr Asn Tyr Tyr Val Gln Lys Ala Leu Asp Ala Thr 260 265 270Pro Glu Glu Leu Glu Lys Gln Gly Gly Tyr Val Phe Leu Tyr Glu Leu 275 280 285Val Lys Gln Thr Arg Asp Pro Lys Val Leu Arg Asp Gln Ser Leu Asn 290 295 300Ile Leu Leu Ala Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Ala305 310 315 320Val Phe Glu Leu Ala Arg Asn Pro His Ile Trp Ala Lys Leu Arg Glu 325 330 335Glu Ile Glu Gln Gln Phe Gly Leu Gly Glu Asp Ser Arg Val Glu Glu 340 345 350Ile Thr Phe Glu Ser Leu Lys Arg Cys Glu Tyr Leu Lys Ala Val Leu 355 360 365Asn Glu Thr Leu Arg Leu His Pro Ser Val Pro Arg Asn Ala Arg Phe 370 375 380Ala Ile Lys Asp Thr Thr Leu Pro Arg Gly Gly Gly Pro Asn Gly Lys385 390 395 400Asp Pro Ile Leu Ile Arg Lys Asp Glu Val Val Gln Tyr Ser Ile Ser 405 410 415Ala Thr Gln Thr Asn Pro Ala Tyr Tyr Gly Ala Asp Ala Ala Asp Phe 420 425 430Arg Pro Glu Arg Trp Phe Glu Pro Ser Thr Arg Asn Leu Gly Trp Ala 435 440 445Phe Leu Pro Phe Asn Gly Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe 450 455 460Ala Leu Thr Glu Ala Gly Tyr Val Leu Val Arg Leu Val Gln Glu Phe465 470

475 480Pro Asn Leu Ser Gln Asp Pro Glu Thr Lys Tyr Pro Pro Pro Arg Leu 485 490 495Ala His Leu Thr Met Cys Leu Phe Asp Gly Ala His Val Lys Met Ser 500 505 510191539DNACandida tropicalisCDS(1)..(1536) 19atg ctc gac cag atc ttc cat tac tgg tac att gtc ttg cca ttg ttg 48Met Leu Asp Gln Ile Phe His Tyr Trp Tyr Ile Val Leu Pro Leu Leu1 5 10 15gtc att atc aag cag atc gtg gct cat gcc agg acc aat tat ttg atg 96Val Ile Ile Lys Gln Ile Val Ala His Ala Arg Thr Asn Tyr Leu Met 20 25 30aag aag ttg ggc gct aag cca ttc aca cat gtc caa cta gac ggg tgg 144Lys Lys Leu Gly Ala Lys Pro Phe Thr His Val Gln Leu Asp Gly Trp 35 40 45ttt ggc ttc aaa ttt ggc cgt gaa ttc ctc aaa gct aaa agt gct ggg 192Phe Gly Phe Lys Phe Gly Arg Glu Phe Leu Lys Ala Lys Ser Ala Gly 50 55 60agg cag gtt gat tta atc atc tcc cgt ttc cac gat aat gag gac act 240Arg Gln Val Asp Leu Ile Ile Ser Arg Phe His Asp Asn Glu Asp Thr65 70 75 80ttc tcc agc tat gct ttt ggc aac cat gtg gtg ttc acc agg gac ccc 288Phe Ser Ser Tyr Ala Phe Gly Asn His Val Val Phe Thr Arg Asp Pro 85 90 95gag aat atc aag gcg ctt ttg gca acc cag ttt ggt gat ttt tca ttg 336Glu Asn Ile Lys Ala Leu Leu Ala Thr Gln Phe Gly Asp Phe Ser Leu 100 105 110gga agc agg gtc aaa ttc ttc aaa cca ttg ttg ggg tac ggt atc ttc 384Gly Ser Arg Val Lys Phe Phe Lys Pro Leu Leu Gly Tyr Gly Ile Phe 115 120 125acc ttg gac ggc gaa ggc tgg aag cac agc aga gcc atg ttg aga cca 432Thr Leu Asp Gly Glu Gly Trp Lys His Ser Arg Ala Met Leu Arg Pro 130 135 140cag ttt gcc aga gag caa gtt gct cat gtg acg tcg ttg gaa cca cat 480Gln Phe Ala Arg Glu Gln Val Ala His Val Thr Ser Leu Glu Pro His145 150 155 160ttc cag ttg ttg aag aag cat att ctt aag cac aag ggt gaa tac ttt 528Phe Gln Leu Leu Lys Lys His Ile Leu Lys His Lys Gly Glu Tyr Phe 165 170 175gat atc cag gaa ttg ttc ttt aga ttt acc gtt gat tca gcg acg gag 576Asp Ile Gln Glu Leu Phe Phe Arg Phe Thr Val Asp Ser Ala Thr Glu 180 185 190ttc tta ttt ggt gag tcc gtg cac tcc tta agg gac gag gaa att ggc 624Phe Leu Phe Gly Glu Ser Val His Ser Leu Arg Asp Glu Glu Ile Gly 195 200 205tac gat acg aag gac atg gct gaa gaa aga cgc aaa ttt gcc gac gcg 672Tyr Asp Thr Lys Asp Met Ala Glu Glu Arg Arg Lys Phe Ala Asp Ala 210 215 220ttc aac aag tcg caa gtc tat ttg tcc acc aga gtt gct tta cag aca 720Phe Asn Lys Ser Gln Val Tyr Leu Ser Thr Arg Val Ala Leu Gln Thr225 230 235 240ttg tac tgg ttg gtc aac aac aaa gag ttc aag gag tgc aac gac att 768Leu Tyr Trp Leu Val Asn Asn Lys Glu Phe Lys Glu Cys Asn Asp Ile 245 250 255gtc cac aag ttc acc aac tac tat gtt cag aaa gcc ttg gat gct acc 816Val His Lys Phe Thr Asn Tyr Tyr Val Gln Lys Ala Leu Asp Ala Thr 260 265 270cca gag gaa ctt gaa aaa caa ggc ggg tat gtg ttc ttg tac gag ctt 864Pro Glu Glu Leu Glu Lys Gln Gly Gly Tyr Val Phe Leu Tyr Glu Leu 275 280 285gcc aag cag acg aaa gac ccc aat gtg ttg cgt gac cag tct ttg aac 912Ala Lys Gln Thr Lys Asp Pro Asn Val Leu Arg Asp Gln Ser Leu Asn 290 295 300atc ttg ttg gct gga agg gac acc act gct ggg ttg ttg tcc ttt gct 960Ile Leu Leu Ala Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Ala305 310 315 320gtg ttt gag ttg gcc agg aac cca cac atc tgg gcc aag ttg aga gag 1008Val Phe Glu Leu Ala Arg Asn Pro His Ile Trp Ala Lys Leu Arg Glu 325 330 335gaa att gaa tca cac ttt ggg ctg ggt gag gac tct cgt gtt gaa gag 1056Glu Ile Glu Ser His Phe Gly Leu Gly Glu Asp Ser Arg Val Glu Glu 340 345 350att acc ttt gag agc ttg aag aga tgt gag tac ttg aaa gcc gtg ttg 1104Ile Thr Phe Glu Ser Leu Lys Arg Cys Glu Tyr Leu Lys Ala Val Leu 355 360 365aac gaa acg ttg aga tta cac cca agt gtc cca aga aac gca aga ttt 1152Asn Glu Thr Leu Arg Leu His Pro Ser Val Pro Arg Asn Ala Arg Phe 370 375 380gcg att aaa gac acg act tta cca aga ggc ggt ggc ccc aac ggc aag 1200Ala Ile Lys Asp Thr Thr Leu Pro Arg Gly Gly Gly Pro Asn Gly Lys385 390 395 400gat cct atc ttg atc aga aag aat gag gtg gtg caa tac tcc atc tcg 1248Asp Pro Ile Leu Ile Arg Lys Asn Glu Val Val Gln Tyr Ser Ile Ser 405 410 415gca act cag aca aat cct gct tat tat ggc gcc gat gct gct gat ttt 1296Ala Thr Gln Thr Asn Pro Ala Tyr Tyr Gly Ala Asp Ala Ala Asp Phe 420 425 430aga ccg gaa aga tgg ttt gag cca tca act aga aac ttg gga tgg gct 1344Arg Pro Glu Arg Trp Phe Glu Pro Ser Thr Arg Asn Leu Gly Trp Ala 435 440 445tac ttg cca ttc aac ggt ggt cca aga atc tgc ttg gga caa cag ttt 1392Tyr Leu Pro Phe Asn Gly Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe 450 455 460gct ttg acc gaa gcc ggt tac gtt ttg gtt aga ctt gtt cag gaa ttc 1440Ala Leu Thr Glu Ala Gly Tyr Val Leu Val Arg Leu Val Gln Glu Phe465 470 475 480cct agc ttg tca cag gac ccc gaa act gag tac cca cca cct aga ttg 1488Pro Ser Leu Ser Gln Asp Pro Glu Thr Glu Tyr Pro Pro Pro Arg Leu 485 490 495gca cac ttg acg atg tgc ttg ttt gac ggg gca tac gtc aag atg caa 1536Ala His Leu Thr Met Cys Leu Phe Asp Gly Ala Tyr Val Lys Met Gln 500 505 510tag 153920512PRTCandida tropicalis 20Met Leu Asp Gln Ile Phe His Tyr Trp Tyr Ile Val Leu Pro Leu Leu1 5 10 15Val Ile Ile Lys Gln Ile Val Ala His Ala Arg Thr Asn Tyr Leu Met 20 25 30Lys Lys Leu Gly Ala Lys Pro Phe Thr His Val Gln Leu Asp Gly Trp 35 40 45Phe Gly Phe Lys Phe Gly Arg Glu Phe Leu Lys Ala Lys Ser Ala Gly 50 55 60Arg Gln Val Asp Leu Ile Ile Ser Arg Phe His Asp Asn Glu Asp Thr65 70 75 80Phe Ser Ser Tyr Ala Phe Gly Asn His Val Val Phe Thr Arg Asp Pro 85 90 95Glu Asn Ile Lys Ala Leu Leu Ala Thr Gln Phe Gly Asp Phe Ser Leu 100 105 110Gly Ser Arg Val Lys Phe Phe Lys Pro Leu Leu Gly Tyr Gly Ile Phe 115 120 125Thr Leu Asp Gly Glu Gly Trp Lys His Ser Arg Ala Met Leu Arg Pro 130 135 140Gln Phe Ala Arg Glu Gln Val Ala His Val Thr Ser Leu Glu Pro His145 150 155 160Phe Gln Leu Leu Lys Lys His Ile Leu Lys His Lys Gly Glu Tyr Phe 165 170 175Asp Ile Gln Glu Leu Phe Phe Arg Phe Thr Val Asp Ser Ala Thr Glu 180 185 190Phe Leu Phe Gly Glu Ser Val His Ser Leu Arg Asp Glu Glu Ile Gly 195 200 205Tyr Asp Thr Lys Asp Met Ala Glu Glu Arg Arg Lys Phe Ala Asp Ala 210 215 220Phe Asn Lys Ser Gln Val Tyr Leu Ser Thr Arg Val Ala Leu Gln Thr225 230 235 240Leu Tyr Trp Leu Val Asn Asn Lys Glu Phe Lys Glu Cys Asn Asp Ile 245 250 255Val His Lys Phe Thr Asn Tyr Tyr Val Gln Lys Ala Leu Asp Ala Thr 260 265 270Pro Glu Glu Leu Glu Lys Gln Gly Gly Tyr Val Phe Leu Tyr Glu Leu 275 280 285Ala Lys Gln Thr Lys Asp Pro Asn Val Leu Arg Asp Gln Ser Leu Asn 290 295 300Ile Leu Leu Ala Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Ala305 310 315 320Val Phe Glu Leu Ala Arg Asn Pro His Ile Trp Ala Lys Leu Arg Glu 325 330 335Glu Ile Glu Ser His Phe Gly Leu Gly Glu Asp Ser Arg Val Glu Glu 340 345 350Ile Thr Phe Glu Ser Leu Lys Arg Cys Glu Tyr Leu Lys Ala Val Leu 355 360 365Asn Glu Thr Leu Arg Leu His Pro Ser Val Pro Arg Asn Ala Arg Phe 370 375 380Ala Ile Lys Asp Thr Thr Leu Pro Arg Gly Gly Gly Pro Asn Gly Lys385 390 395 400Asp Pro Ile Leu Ile Arg Lys Asn Glu Val Val Gln Tyr Ser Ile Ser 405 410 415Ala Thr Gln Thr Asn Pro Ala Tyr Tyr Gly Ala Asp Ala Ala Asp Phe 420 425 430Arg Pro Glu Arg Trp Phe Glu Pro Ser Thr Arg Asn Leu Gly Trp Ala 435 440 445Tyr Leu Pro Phe Asn Gly Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe 450 455 460Ala Leu Thr Glu Ala Gly Tyr Val Leu Val Arg Leu Val Gln Glu Phe465 470 475 480Pro Ser Leu Ser Gln Asp Pro Glu Thr Glu Tyr Pro Pro Pro Arg Leu 485 490 495Ala His Leu Thr Met Cys Leu Phe Asp Gly Ala Tyr Val Lys Met Gln 500 505 51021390DNACandida tropicalisCDS(1)..(387) 21atg acc gac aca gac acc acg acc acc atc tac acc cac gaa gag gtt 48Met Thr Asp Thr Asp Thr Thr Thr Thr Ile Tyr Thr His Glu Glu Val1 5 10 15gcc cag cac acc acc cac gac gac ttg tgg gtt att ctc aat ggt aag 96Ala Gln His Thr Thr His Asp Asp Leu Trp Val Ile Leu Asn Gly Lys 20 25 30gtc tac aac atc tcc aac tat ata gac gag cac cca ggt ggt gaa gaa 144Val Tyr Asn Ile Ser Asn Tyr Ile Asp Glu His Pro Gly Gly Glu Glu 35 40 45gtc att ctt gat tgc gcc ggc aca gac gcc act gaa gcc ttt gac gac 192Val Ile Leu Asp Cys Ala Gly Thr Asp Ala Thr Glu Ala Phe Asp Asp 50 55 60att ggc cac tcc gac gag gcc cac gag atc ttg gaa aag ttg tac att 240Ile Gly His Ser Asp Glu Ala His Glu Ile Leu Glu Lys Leu Tyr Ile65 70 75 80ggt aac ttg aag ggc gct aag att gtt gag gcc aag cac gcg cag tcg 288Gly Asn Leu Lys Gly Ala Lys Ile Val Glu Ala Lys His Ala Gln Ser 85 90 95ttc agc acg gaa gaa gac tcg ggt atc aac ttc cca ttg att gct gtt 336Phe Ser Thr Glu Glu Asp Ser Gly Ile Asn Phe Pro Leu Ile Ala Val 100 105 110ggt gtg ttt ttg gct gct ttc ggt gtc tac tac tac aag acc aac ttt 384Gly Val Phe Leu Ala Ala Phe Gly Val Tyr Tyr Tyr Lys Thr Asn Phe 115 120 125gcc taa 390Ala 22129PRTCandida tropicalis 22Met Thr Asp Thr Asp Thr Thr Thr Thr Ile Tyr Thr His Glu Glu Val1 5 10 15Ala Gln His Thr Thr His Asp Asp Leu Trp Val Ile Leu Asn Gly Lys 20 25 30Val Tyr Asn Ile Ser Asn Tyr Ile Asp Glu His Pro Gly Gly Glu Glu 35 40 45Val Ile Leu Asp Cys Ala Gly Thr Asp Ala Thr Glu Ala Phe Asp Asp 50 55 60Ile Gly His Ser Asp Glu Ala His Glu Ile Leu Glu Lys Leu Tyr Ile65 70 75 80Gly Asn Leu Lys Gly Ala Lys Ile Val Glu Ala Lys His Ala Gln Ser 85 90 95Phe Ser Thr Glu Glu Asp Ser Gly Ile Asn Phe Pro Leu Ile Ala Val 100 105 110Gly Val Phe Leu Ala Ala Phe Gly Val Tyr Tyr Tyr Lys Thr Asn Phe 115 120 125Ala232115DNACandida tropicalisCDS(1)..(2112) 23atg gct cca ttt ttg ccc gac cag gtc gac tac aaa cac gtc gac acc 48Met Ala Pro Phe Leu Pro Asp Gln Val Asp Tyr Lys His Val Asp Thr1 5 10 15ctt atg tta tta tgt gac ggg atc atc cac gaa acc acc gtg gac gaa 96Leu Met Leu Leu Cys Asp Gly Ile Ile His Glu Thr Thr Val Asp Glu 20 25 30atc aaa gac gtc att gcc cct gac ttc ccc gcc gac aaa tac gag gag 144Ile Lys Asp Val Ile Ala Pro Asp Phe Pro Ala Asp Lys Tyr Glu Glu 35 40 45tac gtc agg aca ttc acc aaa ccc tcc gaa acc cca ggg ttc agg gaa 192Tyr Val Arg Thr Phe Thr Lys Pro Ser Glu Thr Pro Gly Phe Arg Glu 50 55 60acc gtc tac aac acc gtc aac gca aac acc atg gat gca atc cac cag 240Thr Val Tyr Asn Thr Val Asn Ala Asn Thr Met Asp Ala Ile His Gln65 70 75 80ttc att atc ttg acc aat gtt ttg gga tca agg gtc ttg gca cca gct 288Phe Ile Ile Leu Thr Asn Val Leu Gly Ser Arg Val Leu Ala Pro Ala 85 90 95ttg acc aac tcg ttg act cct atc aag gac atg agc ttg gaa gac cgt 336Leu Thr Asn Ser Leu Thr Pro Ile Lys Asp Met Ser Leu Glu Asp Arg 100 105 110gaa aag ttg tta gcc tcg tgg cgt gac tcc cct att gct gct aaa agg 384Glu Lys Leu Leu Ala Ser Trp Arg Asp Ser Pro Ile Ala Ala Lys Arg 115 120 125aag ttg ttc agg ttg gtt tct acg ctt acc ttg gtc acg ttc acg aga 432Lys Leu Phe Arg Leu Val Ser Thr Leu Thr Leu Val Thr Phe Thr Arg 130 135 140ttg gcc aat gag ttg cat ttg aaa gcc att cat tat cca gga aga gaa 480Leu Ala Asn Glu Leu His Leu Lys Ala Ile His Tyr Pro Gly Arg Glu145 150 155 160gac cgt gaa aag gct tat gaa acc cag gag att gac cct ttt aag tac 528Asp Arg Glu Lys Ala Tyr Glu Thr Gln Glu Ile Asp Pro Phe Lys Tyr 165 170 175cag ttt ttg gaa aaa ccg aag ttt tac ggc gct gag ttg tac ttg cca 576Gln Phe Leu Glu Lys Pro Lys Phe Tyr Gly Ala Glu Leu Tyr Leu Pro 180 185 190gat att gat gtg atc att att gga tct ggg gcc ggt gct ggt gtc gtg 624Asp Ile Asp Val Ile Ile Ile Gly Ser Gly Ala Gly Ala Gly Val Val 195 200 205gcc cac act ttg acc aac gac ggc ttc aag agt ttg gtt ttg gaa aag 672Ala His Thr Leu Thr Asn Asp Gly Phe Lys Ser Leu Val Leu Glu Lys 210 215 220ggc aga tac ttt agc aac tcc gag ttg aac ttt gat gac aag gac ggg 720Gly Arg Tyr Phe Ser Asn Ser Glu Leu Asn Phe Asp Asp Lys Asp Gly225 230 235 240gtt caa gaa tta tac caa agt gga ggt act ttg acc acc gtc aac cag 768Val Gln Glu Leu Tyr Gln Ser Gly Gly Thr Leu Thr Thr Val Asn Gln 245 250 255cag ttg ttt gtt ctt gct ggt tcc act ttt ggt ggt ggt acc act gtc 816Gln Leu Phe Val Leu Ala Gly Ser Thr Phe Gly Gly Gly Thr Thr Val 260 265 270aat tgg tcg gcc tgt ctt aaa acg cca ttc aag gtg cgt aag gaa tgg 864Asn Trp Ser Ala Cys Leu Lys Thr Pro Phe Lys Val Arg Lys Glu Trp 275 280 285tat gat gag ttt ggc gtt gac ttt gct gcc gat gaa gcc tac gac aaa 912Tyr Asp Glu Phe Gly Val Asp Phe Ala Ala Asp Glu Ala Tyr Asp Lys 290 295 300gca cag gat tat gtt tgg cag caa atg gga gct tct acc gaa ggc atc 960Ala Gln Asp Tyr Val Trp Gln Gln Met Gly Ala Ser Thr Glu Gly Ile305 310 315 320acc cac tct ttg gct aac gag att att att gaa ggt ggc aag aaa tta 1008Thr His Ser Leu Ala Asn Glu Ile Ile Ile Glu Gly Gly Lys Lys Leu 325 330 335ggt tac aag gcc aag gta tta gac caa aac agc ggt ggt cat cct cat 1056Gly Tyr Lys Ala Lys Val Leu Asp Gln Asn Ser Gly Gly His Pro His 340 345 350cac aga tgc ggt ttc tgt tat ttg ggt tgt aag cac ggt atc aag cag 1104His Arg Cys Gly Phe Cys Tyr Leu Gly Cys Lys His Gly Ile Lys Gln 355 360 365ggc tct gtt aat aac tgg ttt aga gac gca gct gcc cac ggt tct cag 1152Gly Ser Val Asn Asn Trp Phe Arg Asp Ala Ala Ala His Gly Ser Gln 370 375 380ttc atg caa cag gtt aga gtt ttg caa atc ctt aac aag aag ggc atc 1200Phe Met Gln Gln Val Arg Val Leu Gln Ile Leu Asn Lys Lys Gly Ile385 390 395 400gct tat ggt atc ttg tgt gag gat gtt gta acc ggt gcc aag ttc acc 1248Ala Tyr Gly Ile Leu Cys Glu Asp Val Val Thr Gly Ala Lys Phe Thr 405 410 415att act ggc ccc aaa aag ttt gtt gtt gcc gcc ggc gcc tta aac act 1296Ile Thr Gly Pro Lys Lys Phe Val Val Ala Ala Gly Ala Leu Asn Thr 420 425 430cca tct gtg ttg gtc aac tcc gga ttc aag aac aag aac atc ggt aag 1344Pro Ser Val Leu Val Asn Ser Gly Phe Lys Asn Lys Asn Ile Gly Lys 435 440 445aac tta act ttg cat cca gtt

tct gtc gtg ttt ggt gat ttt ggc aaa 1392Asn Leu Thr Leu His Pro Val Ser Val Val Phe Gly Asp Phe Gly Lys 450 455 460gac gtt caa gca gat cac ttc cac aac tcc atc atg act gct ctt tgt 1440Asp Val Gln Ala Asp His Phe His Asn Ser Ile Met Thr Ala Leu Cys465 470 475 480tca gaa gcc gct gat tta gac ggc aag ggt cat gga tgc aga att gaa 1488Ser Glu Ala Ala Asp Leu Asp Gly Lys Gly His Gly Cys Arg Ile Glu 485 490 495acc atc ttg aac gct cca ttc atc cag gct tca ttc tta cca tgg aga 1536Thr Ile Leu Asn Ala Pro Phe Ile Gln Ala Ser Phe Leu Pro Trp Arg 500 505 510ggt agt aac gag gct aga cga gac ttg ttg cgt tac aac aac atg gtg 1584Gly Ser Asn Glu Ala Arg Arg Asp Leu Leu Arg Tyr Asn Asn Met Val 515 520 525gcc atg tta ctt ctt agt cgt gat acc acc agt ggt tcc gtt tcg tcc 1632Ala Met Leu Leu Leu Ser Arg Asp Thr Thr Ser Gly Ser Val Ser Ser 530 535 540cat cca act aaa cct gaa gca tta gtt gtc gag tac gac gtg aac aag 1680His Pro Thr Lys Pro Glu Ala Leu Val Val Glu Tyr Asp Val Asn Lys545 550 555 560ttt gac aga aac tcc atc ttg cag gca ttg ttg gtc act gct gac ttg 1728Phe Asp Arg Asn Ser Ile Leu Gln Ala Leu Leu Val Thr Ala Asp Leu 565 570 575ttg tac att caa ggt gcc aag aga atc ctt agt ccc caa cca tgg gtg 1776Leu Tyr Ile Gln Gly Ala Lys Arg Ile Leu Ser Pro Gln Pro Trp Val 580 585 590cca att ttt gaa tcc gac aag cca aag gat aag aga tca atc aag gac 1824Pro Ile Phe Glu Ser Asp Lys Pro Lys Asp Lys Arg Ser Ile Lys Asp 595 600 605gag gac tat gtc gaa tgg aga gcc aag gtt gcc aag att cct ttt gac 1872Glu Asp Tyr Val Glu Trp Arg Ala Lys Val Ala Lys Ile Pro Phe Asp 610 615 620acc tac ggc tcg cct tat ggt tcg gcg cat caa atg tct tct tgt cgt 1920Thr Tyr Gly Ser Pro Tyr Gly Ser Ala His Gln Met Ser Ser Cys Arg625 630 635 640atg tca ggt aag ggt cct aaa tac ggt gct gtt gat acc gat ggt aga 1968Met Ser Gly Lys Gly Pro Lys Tyr Gly Ala Val Asp Thr Asp Gly Arg 645 650 655ttg ttt gaa tgt tcg aat gtt tat gtt gct gac gct agt ctt ttg cca 2016Leu Phe Glu Cys Ser Asn Val Tyr Val Ala Asp Ala Ser Leu Leu Pro 660 665 670act gct agc ggt gct aat cct atg gtc acc acc atg act ctt gca aga 2064Thr Ala Ser Gly Ala Asn Pro Met Val Thr Thr Met Thr Leu Ala Arg 675 680 685cat gtt gcg tta ggt ttg gca gac tcc ttg aag acc aag gcc aag ttg 2112His Val Ala Leu Gly Leu Ala Asp Ser Leu Lys Thr Lys Ala Lys Leu 690 695 700tag 211524704PRTCandida tropicalis 24Met Ala Pro Phe Leu Pro Asp Gln Val Asp Tyr Lys His Val Asp Thr1 5 10 15Leu Met Leu Leu Cys Asp Gly Ile Ile His Glu Thr Thr Val Asp Glu 20 25 30Ile Lys Asp Val Ile Ala Pro Asp Phe Pro Ala Asp Lys Tyr Glu Glu 35 40 45Tyr Val Arg Thr Phe Thr Lys Pro Ser Glu Thr Pro Gly Phe Arg Glu 50 55 60Thr Val Tyr Asn Thr Val Asn Ala Asn Thr Met Asp Ala Ile His Gln65 70 75 80Phe Ile Ile Leu Thr Asn Val Leu Gly Ser Arg Val Leu Ala Pro Ala 85 90 95Leu Thr Asn Ser Leu Thr Pro Ile Lys Asp Met Ser Leu Glu Asp Arg 100 105 110Glu Lys Leu Leu Ala Ser Trp Arg Asp Ser Pro Ile Ala Ala Lys Arg 115 120 125Lys Leu Phe Arg Leu Val Ser Thr Leu Thr Leu Val Thr Phe Thr Arg 130 135 140Leu Ala Asn Glu Leu His Leu Lys Ala Ile His Tyr Pro Gly Arg Glu145 150 155 160Asp Arg Glu Lys Ala Tyr Glu Thr Gln Glu Ile Asp Pro Phe Lys Tyr 165 170 175Gln Phe Leu Glu Lys Pro Lys Phe Tyr Gly Ala Glu Leu Tyr Leu Pro 180 185 190Asp Ile Asp Val Ile Ile Ile Gly Ser Gly Ala Gly Ala Gly Val Val 195 200 205Ala His Thr Leu Thr Asn Asp Gly Phe Lys Ser Leu Val Leu Glu Lys 210 215 220Gly Arg Tyr Phe Ser Asn Ser Glu Leu Asn Phe Asp Asp Lys Asp Gly225 230 235 240Val Gln Glu Leu Tyr Gln Ser Gly Gly Thr Leu Thr Thr Val Asn Gln 245 250 255Gln Leu Phe Val Leu Ala Gly Ser Thr Phe Gly Gly Gly Thr Thr Val 260 265 270Asn Trp Ser Ala Cys Leu Lys Thr Pro Phe Lys Val Arg Lys Glu Trp 275 280 285Tyr Asp Glu Phe Gly Val Asp Phe Ala Ala Asp Glu Ala Tyr Asp Lys 290 295 300Ala Gln Asp Tyr Val Trp Gln Gln Met Gly Ala Ser Thr Glu Gly Ile305 310 315 320Thr His Ser Leu Ala Asn Glu Ile Ile Ile Glu Gly Gly Lys Lys Leu 325 330 335Gly Tyr Lys Ala Lys Val Leu Asp Gln Asn Ser Gly Gly His Pro His 340 345 350His Arg Cys Gly Phe Cys Tyr Leu Gly Cys Lys His Gly Ile Lys Gln 355 360 365Gly Ser Val Asn Asn Trp Phe Arg Asp Ala Ala Ala His Gly Ser Gln 370 375 380Phe Met Gln Gln Val Arg Val Leu Gln Ile Leu Asn Lys Lys Gly Ile385 390 395 400Ala Tyr Gly Ile Leu Cys Glu Asp Val Val Thr Gly Ala Lys Phe Thr 405 410 415Ile Thr Gly Pro Lys Lys Phe Val Val Ala Ala Gly Ala Leu Asn Thr 420 425 430Pro Ser Val Leu Val Asn Ser Gly Phe Lys Asn Lys Asn Ile Gly Lys 435 440 445Asn Leu Thr Leu His Pro Val Ser Val Val Phe Gly Asp Phe Gly Lys 450 455 460Asp Val Gln Ala Asp His Phe His Asn Ser Ile Met Thr Ala Leu Cys465 470 475 480Ser Glu Ala Ala Asp Leu Asp Gly Lys Gly His Gly Cys Arg Ile Glu 485 490 495Thr Ile Leu Asn Ala Pro Phe Ile Gln Ala Ser Phe Leu Pro Trp Arg 500 505 510Gly Ser Asn Glu Ala Arg Arg Asp Leu Leu Arg Tyr Asn Asn Met Val 515 520 525Ala Met Leu Leu Leu Ser Arg Asp Thr Thr Ser Gly Ser Val Ser Ser 530 535 540His Pro Thr Lys Pro Glu Ala Leu Val Val Glu Tyr Asp Val Asn Lys545 550 555 560Phe Asp Arg Asn Ser Ile Leu Gln Ala Leu Leu Val Thr Ala Asp Leu 565 570 575Leu Tyr Ile Gln Gly Ala Lys Arg Ile Leu Ser Pro Gln Pro Trp Val 580 585 590Pro Ile Phe Glu Ser Asp Lys Pro Lys Asp Lys Arg Ser Ile Lys Asp 595 600 605Glu Asp Tyr Val Glu Trp Arg Ala Lys Val Ala Lys Ile Pro Phe Asp 610 615 620Thr Tyr Gly Ser Pro Tyr Gly Ser Ala His Gln Met Ser Ser Cys Arg625 630 635 640Met Ser Gly Lys Gly Pro Lys Tyr Gly Ala Val Asp Thr Asp Gly Arg 645 650 655Leu Phe Glu Cys Ser Asn Val Tyr Val Ala Asp Ala Ser Leu Leu Pro 660 665 670Thr Ala Ser Gly Ala Asn Pro Met Val Thr Thr Met Thr Leu Ala Arg 675 680 685His Val Ala Leu Gly Leu Ala Asp Ser Leu Lys Thr Lys Ala Lys Leu 690 695 700252115DNACandida tropicalisCDS(1)..(2112) 25atg aat acc ttc ttg cca gac gtg ctc gaa tac aaa cac gtc gac acc 48Met Asn Thr Phe Leu Pro Asp Val Leu Glu Tyr Lys His Val Asp Thr1 5 10 15ctt ttg tta ttg tgt gac ggg atc atc cac gaa acc aca gtc gat cag 96Leu Leu Leu Leu Cys Asp Gly Ile Ile His Glu Thr Thr Val Asp Gln 20 25 30atc aag gac gcc att gct ccc gac ttc cct gag gac cag tac gag gag 144Ile Lys Asp Ala Ile Ala Pro Asp Phe Pro Glu Asp Gln Tyr Glu Glu 35 40 45tat ctc aag acc ttc acc aag cca tct gag acc cct ggg ttc aga gaa 192Tyr Leu Lys Thr Phe Thr Lys Pro Ser Glu Thr Pro Gly Phe Arg Glu 50 55 60gcc gtc tac gac acg atc aac gcc acc cca acc gat gcc gtg cac atg 240Ala Val Tyr Asp Thr Ile Asn Ala Thr Pro Thr Asp Ala Val His Met65 70 75 80tgt att gtc ttg acc acc gca ttg gac tcc aga atc ttg gcc ccc acg 288Cys Ile Val Leu Thr Thr Ala Leu Asp Ser Arg Ile Leu Ala Pro Thr 85 90 95ttg acc aac tcg ttg acg cct atc aag gat atg acc ttg aag gag cgt 336Leu Thr Asn Ser Leu Thr Pro Ile Lys Asp Met Thr Leu Lys Glu Arg 100 105 110gaa caa ttg ttg gcc tct tgg cgt gat tcc ccg att gcg gca aag aga 384Glu Gln Leu Leu Ala Ser Trp Arg Asp Ser Pro Ile Ala Ala Lys Arg 115 120 125aga ttg ttc aga ttg att tcc tcg ctt acc ttg acg acg ttt acg aga 432Arg Leu Phe Arg Leu Ile Ser Ser Leu Thr Leu Thr Thr Phe Thr Arg 130 135 140ttg gcc agc gaa ttg cac ttg aaa gcc atc cac tac cct ggc aga gac 480Leu Ala Ser Glu Leu His Leu Lys Ala Ile His Tyr Pro Gly Arg Asp145 150 155 160ttg cgt gaa aag gcg tat gaa acc cag gtg gtt gac cct ttc agg tac 528Leu Arg Glu Lys Ala Tyr Glu Thr Gln Val Val Asp Pro Phe Arg Tyr 165 170 175ctg ttt atg gag aaa cca aag ttt gac ggc gcc gaa ttg tac ttg cca 576Leu Phe Met Glu Lys Pro Lys Phe Asp Gly Ala Glu Leu Tyr Leu Pro 180 185 190gat atc gac gtc atc atc att gga tca ggc gcc ggt gct ggt gtc atg 624Asp Ile Asp Val Ile Ile Ile Gly Ser Gly Ala Gly Ala Gly Val Met 195 200 205gcc cac act ctc gcc aac gac ggg ttc aag acc ttg gtt ttg gaa aag 672Ala His Thr Leu Ala Asn Asp Gly Phe Lys Thr Leu Val Leu Glu Lys 210 215 220gga aag tat ttc agc aac tcc gag ttg aac ttt aat gac gct gat ggc 720Gly Lys Tyr Phe Ser Asn Ser Glu Leu Asn Phe Asn Asp Ala Asp Gly225 230 235 240gtg aaa gag ttg tac caa ggt aaa ggt gct ttg gcc acc acc aat cag 768Val Lys Glu Leu Tyr Gln Gly Lys Gly Ala Leu Ala Thr Thr Asn Gln 245 250 255cag atg ttt att ctt gcc ggt tcc act ttg ggc ggt ggt acc act gtc 816Gln Met Phe Ile Leu Ala Gly Ser Thr Leu Gly Gly Gly Thr Thr Val 260 265 270aac tgg tct gct tgc ctt aaa aca cca ttt aaa gtg cgt aag gag tgg 864Asn Trp Ser Ala Cys Leu Lys Thr Pro Phe Lys Val Arg Lys Glu Trp 275 280 285tac gac gag ttt ggt ctt gaa ttt gct gcc gat gaa gcc tac gac aaa 912Tyr Asp Glu Phe Gly Leu Glu Phe Ala Ala Asp Glu Ala Tyr Asp Lys 290 295 300gcg cag gat tat gtt tgg aaa caa atg ggt gct tca aca gat gga atc 960Ala Gln Asp Tyr Val Trp Lys Gln Met Gly Ala Ser Thr Asp Gly Ile305 310 315 320act cac tcc ttg gcc aac gaa gtt gtg gtt gaa gga ggt aag aag ttg 1008Thr His Ser Leu Ala Asn Glu Val Val Val Glu Gly Gly Lys Lys Leu 325 330 335ggc tac aag agc aag gaa att gag cag aac aac ggt ggc cac cct gac 1056Gly Tyr Lys Ser Lys Glu Ile Glu Gln Asn Asn Gly Gly His Pro Asp 340 345 350cac cca tgt ggt ttc tgt tac ttg ggc tgt aag tac ggt att aaa cag 1104His Pro Cys Gly Phe Cys Tyr Leu Gly Cys Lys Tyr Gly Ile Lys Gln 355 360 365ggt tct gtg aat aac tgg ttt aga gac gca gct gcc cac ggg tcc aag 1152Gly Ser Val Asn Asn Trp Phe Arg Asp Ala Ala Ala His Gly Ser Lys 370 375 380ttc atg caa caa gtc aga gtt gtg caa atc ctc aac aag aat ggc gtc 1200Phe Met Gln Gln Val Arg Val Val Gln Ile Leu Asn Lys Asn Gly Val385 390 395 400gct tat ggt atc ttg tgt gag gat gtc gaa acc gga gtc agg ttc act 1248Ala Tyr Gly Ile Leu Cys Glu Asp Val Glu Thr Gly Val Arg Phe Thr 405 410 415att agt ggc ccc aaa aag ttt gtt gtt tct gct ggt tct ttg aac acg 1296Ile Ser Gly Pro Lys Lys Phe Val Val Ser Ala Gly Ser Leu Asn Thr 420 425 430cca act gtg ttg acc aac tcc gga ttc aag aac aag cac att ggt aag 1344Pro Thr Val Leu Thr Asn Ser Gly Phe Lys Asn Lys His Ile Gly Lys 435 440 445aac ttg acg ttg cac cca gtt tcc acc gtg ttt ggt gac ttt ggc aga 1392Asn Leu Thr Leu His Pro Val Ser Thr Val Phe Gly Asp Phe Gly Arg 450 455 460gac gtg caa gcc gac cat ttc cac aaa tct att atg act tcg ctt tgt 1440Asp Val Gln Ala Asp His Phe His Lys Ser Ile Met Thr Ser Leu Cys465 470 475 480tac gag gtt gct gac ttg gac ggc aag ggc cac gga tgc aga atc gaa 1488Tyr Glu Val Ala Asp Leu Asp Gly Lys Gly His Gly Cys Arg Ile Glu 485 490 495acc atc ttg aac gct cca ttc atc caa gct tct ttg ttg cca tgg aga 1536Thr Ile Leu Asn Ala Pro Phe Ile Gln Ala Ser Leu Leu Pro Trp Arg 500 505 510gga agt gac gag gtc aga aga gac ttg ttg cgt tac aac aac atg gtg 1584Gly Ser Asp Glu Val Arg Arg Asp Leu Leu Arg Tyr Asn Asn Met Val 515 520 525gcc atg ttg ctt atc acg cgt gat acc acc agt ggt tca gtt tct gct 1632Ala Met Leu Leu Ile Thr Arg Asp Thr Thr Ser Gly Ser Val Ser Ala 530 535 540gac cca aag aag ccc gac gct ttg att gtc gac tat gag att aac aag 1680Asp Pro Lys Lys Pro Asp Ala Leu Ile Val Asp Tyr Glu Ile Asn Lys545 550 555 560ttt gac aag aat gcc atc ttg caa gct ttc ttg atc act tcc gac atg 1728Phe Asp Lys Asn Ala Ile Leu Gln Ala Phe Leu Ile Thr Ser Asp Met 565 570 575ttg tac att gaa ggt gcc aag aga atc ctc agt cca cag cca tgg gtg 1776Leu Tyr Ile Glu Gly Ala Lys Arg Ile Leu Ser Pro Gln Pro Trp Val 580 585 590cca atc ttt gag tcg aac aag cca aag gag caa aga acg atc aag gac 1824Pro Ile Phe Glu Ser Asn Lys Pro Lys Glu Gln Arg Thr Ile Lys Asp 595 600 605aag gac tat gtt gag tgg aga gcc aag gct gct aag ata cct ttc gac 1872Lys Asp Tyr Val Glu Trp Arg Ala Lys Ala Ala Lys Ile Pro Phe Asp 610 615 620acc tac ggt tct gca tat ggg tcc gca cat caa atg tcc acc tgt cgt 1920Thr Tyr Gly Ser Ala Tyr Gly Ser Ala His Gln Met Ser Thr Cys Arg625 630 635 640atg tcc gga aag ggt cct aaa tac ggt gct gtt gat act gat ggt aga 1968Met Ser Gly Lys Gly Pro Lys Tyr Gly Ala Val Asp Thr Asp Gly Arg 645 650 655ttg ttt gaa tgt tcg aat gtc tat gtt gct gat gct agt gtt ttg cct 2016Leu Phe Glu Cys Ser Asn Val Tyr Val Ala Asp Ala Ser Val Leu Pro 660 665 670act gcc agc ggt gcc aac cca atg ata tcc acc atg acc ttt gct aga 2064Thr Ala Ser Gly Ala Asn Pro Met Ile Ser Thr Met Thr Phe Ala Arg 675 680 685cag att gcg tta ggt ttg gct gac tcc ttg aag acc aaa ccc aag ttg 2112Gln Ile Ala Leu Gly Leu Ala Asp Ser Leu Lys Thr Lys Pro Lys Leu 690 695 700tag 211526704PRTCandida tropicalis 26Met Asn Thr Phe Leu Pro Asp Val Leu Glu Tyr Lys His Val Asp Thr1 5 10 15Leu Leu Leu Leu Cys Asp Gly Ile Ile His Glu Thr Thr Val Asp Gln 20 25 30Ile Lys Asp Ala Ile Ala Pro Asp Phe Pro Glu Asp Gln Tyr Glu Glu 35 40 45Tyr Leu Lys Thr Phe Thr Lys Pro Ser Glu Thr Pro Gly Phe Arg Glu 50 55 60Ala Val Tyr Asp Thr Ile Asn Ala Thr Pro Thr Asp Ala Val His Met65 70 75 80Cys Ile Val Leu Thr Thr Ala Leu Asp Ser Arg Ile Leu Ala Pro Thr 85 90 95Leu Thr Asn Ser Leu Thr Pro Ile Lys Asp Met Thr Leu Lys Glu Arg 100 105 110Glu Gln Leu Leu Ala Ser Trp Arg Asp Ser Pro Ile Ala Ala Lys Arg 115 120 125Arg Leu Phe Arg Leu Ile Ser Ser Leu Thr Leu Thr Thr Phe Thr Arg 130 135 140Leu Ala Ser Glu Leu His Leu Lys Ala Ile His Tyr Pro Gly Arg Asp145 150 155 160Leu Arg Glu Lys Ala Tyr Glu Thr Gln Val Val Asp Pro Phe Arg Tyr 165 170 175Leu Phe Met Glu Lys Pro Lys Phe Asp Gly Ala Glu Leu Tyr Leu Pro 180 185 190Asp Ile Asp Val Ile Ile Ile Gly Ser Gly Ala Gly Ala Gly Val Met 195 200

205Ala His Thr Leu Ala Asn Asp Gly Phe Lys Thr Leu Val Leu Glu Lys 210 215 220Gly Lys Tyr Phe Ser Asn Ser Glu Leu Asn Phe Asn Asp Ala Asp Gly225 230 235 240Val Lys Glu Leu Tyr Gln Gly Lys Gly Ala Leu Ala Thr Thr Asn Gln 245 250 255Gln Met Phe Ile Leu Ala Gly Ser Thr Leu Gly Gly Gly Thr Thr Val 260 265 270Asn Trp Ser Ala Cys Leu Lys Thr Pro Phe Lys Val Arg Lys Glu Trp 275 280 285Tyr Asp Glu Phe Gly Leu Glu Phe Ala Ala Asp Glu Ala Tyr Asp Lys 290 295 300Ala Gln Asp Tyr Val Trp Lys Gln Met Gly Ala Ser Thr Asp Gly Ile305 310 315 320Thr His Ser Leu Ala Asn Glu Val Val Val Glu Gly Gly Lys Lys Leu 325 330 335Gly Tyr Lys Ser Lys Glu Ile Glu Gln Asn Asn Gly Gly His Pro Asp 340 345 350His Pro Cys Gly Phe Cys Tyr Leu Gly Cys Lys Tyr Gly Ile Lys Gln 355 360 365Gly Ser Val Asn Asn Trp Phe Arg Asp Ala Ala Ala His Gly Ser Lys 370 375 380Phe Met Gln Gln Val Arg Val Val Gln Ile Leu Asn Lys Asn Gly Val385 390 395 400Ala Tyr Gly Ile Leu Cys Glu Asp Val Glu Thr Gly Val Arg Phe Thr 405 410 415Ile Ser Gly Pro Lys Lys Phe Val Val Ser Ala Gly Ser Leu Asn Thr 420 425 430Pro Thr Val Leu Thr Asn Ser Gly Phe Lys Asn Lys His Ile Gly Lys 435 440 445Asn Leu Thr Leu His Pro Val Ser Thr Val Phe Gly Asp Phe Gly Arg 450 455 460Asp Val Gln Ala Asp His Phe His Lys Ser Ile Met Thr Ser Leu Cys465 470 475 480Tyr Glu Val Ala Asp Leu Asp Gly Lys Gly His Gly Cys Arg Ile Glu 485 490 495Thr Ile Leu Asn Ala Pro Phe Ile Gln Ala Ser Leu Leu Pro Trp Arg 500 505 510Gly Ser Asp Glu Val Arg Arg Asp Leu Leu Arg Tyr Asn Asn Met Val 515 520 525Ala Met Leu Leu Ile Thr Arg Asp Thr Thr Ser Gly Ser Val Ser Ala 530 535 540Asp Pro Lys Lys Pro Asp Ala Leu Ile Val Asp Tyr Glu Ile Asn Lys545 550 555 560Phe Asp Lys Asn Ala Ile Leu Gln Ala Phe Leu Ile Thr Ser Asp Met 565 570 575Leu Tyr Ile Glu Gly Ala Lys Arg Ile Leu Ser Pro Gln Pro Trp Val 580 585 590Pro Ile Phe Glu Ser Asn Lys Pro Lys Glu Gln Arg Thr Ile Lys Asp 595 600 605Lys Asp Tyr Val Glu Trp Arg Ala Lys Ala Ala Lys Ile Pro Phe Asp 610 615 620Thr Tyr Gly Ser Ala Tyr Gly Ser Ala His Gln Met Ser Thr Cys Arg625 630 635 640Met Ser Gly Lys Gly Pro Lys Tyr Gly Ala Val Asp Thr Asp Gly Arg 645 650 655Leu Phe Glu Cys Ser Asn Val Tyr Val Ala Asp Ala Ser Val Leu Pro 660 665 670Thr Ala Ser Gly Ala Asn Pro Met Ile Ser Thr Met Thr Phe Ala Arg 675 680 685Gln Ile Ala Leu Gly Leu Ala Asp Ser Leu Lys Thr Lys Pro Lys Leu 690 695 700272115DNACandida tropicalisCDS(1)..(2112) 27atg aat acc ttc ttg cca gac gtg ctc gaa tac aaa cac gtc gat acc 48Met Asn Thr Phe Leu Pro Asp Val Leu Glu Tyr Lys His Val Asp Thr1 5 10 15ctt ttg tta tta tgt gac ggg atc atc cac gaa acc aca gtc gac cag 96Leu Leu Leu Leu Cys Asp Gly Ile Ile His Glu Thr Thr Val Asp Gln 20 25 30atc agg gac gcc att gct ccc gac ttc cct gaa gac cag tac gag gag 144Ile Arg Asp Ala Ile Ala Pro Asp Phe Pro Glu Asp Gln Tyr Glu Glu 35 40 45tat ctc aag acc ttc acc aag cca tct gag acc cct ggg ttc aga gaa 192Tyr Leu Lys Thr Phe Thr Lys Pro Ser Glu Thr Pro Gly Phe Arg Glu 50 55 60gcc gtc tac gac acg atc aac agc acc cca acc gag gct gtg cac atg 240Ala Val Tyr Asp Thr Ile Asn Ser Thr Pro Thr Glu Ala Val His Met65 70 75 80tgt att gta ttg acc acc gca ttg gac tcg aga atc ttg gcc ccc acg 288Cys Ile Val Leu Thr Thr Ala Leu Asp Ser Arg Ile Leu Ala Pro Thr 85 90 95ttg acc aac tcg ttg acg cct atc aag gat atg acc ttg aaa gag cgt 336Leu Thr Asn Ser Leu Thr Pro Ile Lys Asp Met Thr Leu Lys Glu Arg 100 105 110gaa caa ttg ttg gct gcc tgg cgt gat tcc ccg atc gcg gcc aag aga 384Glu Gln Leu Leu Ala Ala Trp Arg Asp Ser Pro Ile Ala Ala Lys Arg 115 120 125aga ttg ttc aga ttg att tcc tca ctt acc ttg acg acc ttt acg aga 432Arg Leu Phe Arg Leu Ile Ser Ser Leu Thr Leu Thr Thr Phe Thr Arg 130 135 140ttg gcc agc gac ttg cac ttg aga gcc atc cac tac cct ggc aga gac 480Leu Ala Ser Asp Leu His Leu Arg Ala Ile His Tyr Pro Gly Arg Asp145 150 155 160ttg cgt gaa aag gca tat gaa acc cag gtg gtt gac cct ttc agg tac 528Leu Arg Glu Lys Ala Tyr Glu Thr Gln Val Val Asp Pro Phe Arg Tyr 165 170 175ctg ttt atg gaa aaa cca aag ttt gac ggc acc gag ttg tac ttg cca 576Leu Phe Met Glu Lys Pro Lys Phe Asp Gly Thr Glu Leu Tyr Leu Pro 180 185 190gat atc gac gtc atc atc att gga tcc ggt gcc ggt gct ggt gtc atg 624Asp Ile Asp Val Ile Ile Ile Gly Ser Gly Ala Gly Ala Gly Val Met 195 200 205gcc cac act tta gcc aac gac ggg tac aag acc ttg gtt ttg gaa aag 672Ala His Thr Leu Ala Asn Asp Gly Tyr Lys Thr Leu Val Leu Glu Lys 210 215 220gga aag tat ttc agc aac tcc gag ttg aac ttt aat gat gcc gat ggt 720Gly Lys Tyr Phe Ser Asn Ser Glu Leu Asn Phe Asn Asp Ala Asp Gly225 230 235 240atg aaa gag ttg tac caa ggt aaa tgt gcg ttg acc acc acg aac cag 768Met Lys Glu Leu Tyr Gln Gly Lys Cys Ala Leu Thr Thr Thr Asn Gln 245 250 255cag atg ttt att ctt gcc ggt tcc act ttg ggc ggt ggt acc act gtt 816Gln Met Phe Ile Leu Ala Gly Ser Thr Leu Gly Gly Gly Thr Thr Val 260 265 270aac tgg tct gct tgt ctt aaa aca cca ttt aaa gtg cgt aag gag tgg 864Asn Trp Ser Ala Cys Leu Lys Thr Pro Phe Lys Val Arg Lys Glu Trp 275 280 285tac gac gag ttt ggt ctt gaa ttt gct gcc gac gaa gcc tac gac aaa 912Tyr Asp Glu Phe Gly Leu Glu Phe Ala Ala Asp Glu Ala Tyr Asp Lys 290 295 300gca caa gac tat gtt tgg aaa caa atg ggc gct tct acc gaa gga atc 960Ala Gln Asp Tyr Val Trp Lys Gln Met Gly Ala Ser Thr Glu Gly Ile305 310 315 320act cac tct ttg gcg aac gcg gtt gtg gtt gaa gga ggt aag aag ttg 1008Thr His Ser Leu Ala Asn Ala Val Val Val Glu Gly Gly Lys Lys Leu 325 330 335ggt tac aag agc aag gaa atc gag cag aac aat ggt ggc cat cct gac 1056Gly Tyr Lys Ser Lys Glu Ile Glu Gln Asn Asn Gly Gly His Pro Asp 340 345 350cac ccc tgt ggt ttc tgt tac ttg ggc tgt aag tac ggt att aag cag 1104His Pro Cys Gly Phe Cys Tyr Leu Gly Cys Lys Tyr Gly Ile Lys Gln 355 360 365ggt tct gtg aat aac tgg ttt aga gac gca gct gcc cac ggg tcc aag 1152Gly Ser Val Asn Asn Trp Phe Arg Asp Ala Ala Ala His Gly Ser Lys 370 375 380ttc atg caa caa gtc aga gtt gtg caa atc ctc cac aat aaa ggc gtc 1200Phe Met Gln Gln Val Arg Val Val Gln Ile Leu His Asn Lys Gly Val385 390 395 400gct tat ggc atc ttg tgt gag gat gtc gag acc gga gtc aaa ttc act 1248Ala Tyr Gly Ile Leu Cys Glu Asp Val Glu Thr Gly Val Lys Phe Thr 405 410 415atc agt ggc ccc aaa aag ttt gtt gtt tct gca ggt tct ttg aac acg 1296Ile Ser Gly Pro Lys Lys Phe Val Val Ser Ala Gly Ser Leu Asn Thr 420 425 430cca acg gtg ttg acc aac tcc gga ttc aag aac aaa cac atc ggt aag 1344Pro Thr Val Leu Thr Asn Ser Gly Phe Lys Asn Lys His Ile Gly Lys 435 440 445aac ttg acg ttg cac cca gtt tcg acc gtg ttt ggt gac ttt ggc aga 1392Asn Leu Thr Leu His Pro Val Ser Thr Val Phe Gly Asp Phe Gly Arg 450 455 460gac gtg caa gcc gac cat ttc cac aaa tct att atg act tcg ctc tgt 1440Asp Val Gln Ala Asp His Phe His Lys Ser Ile Met Thr Ser Leu Cys465 470 475 480tac gaa gtc gct gac ttg gac ggc aag ggc cac gga tgc aga atc gag 1488Tyr Glu Val Ala Asp Leu Asp Gly Lys Gly His Gly Cys Arg Ile Glu 485 490 495acc atc ttg aac gct cca ttc atc caa gct tct ttg ttg cca tgg aga 1536Thr Ile Leu Asn Ala Pro Phe Ile Gln Ala Ser Leu Leu Pro Trp Arg 500 505 510gga agc gac gag gtc aga aga gac ttg ttg cgt tac aac aac atg gtg 1584Gly Ser Asp Glu Val Arg Arg Asp Leu Leu Arg Tyr Asn Asn Met Val 515 520 525gcc atg ttg ctt atc acc cgt gac acc acc agt ggt tca gtt tct gct 1632Ala Met Leu Leu Ile Thr Arg Asp Thr Thr Ser Gly Ser Val Ser Ala 530 535 540gac cca aag aag ccc gac gct ttg att gtc gac tat gac atc aac aag 1680Asp Pro Lys Lys Pro Asp Ala Leu Ile Val Asp Tyr Asp Ile Asn Lys545 550 555 560ttt gac aag aat gcc atc ttg caa gct ttc ttg atc acc tcc gac atg 1728Phe Asp Lys Asn Ala Ile Leu Gln Ala Phe Leu Ile Thr Ser Asp Met 565 570 575ttg tac atc gaa ggt gcc aag aga atc ctc agt cca cag gca tgg gtg 1776Leu Tyr Ile Glu Gly Ala Lys Arg Ile Leu Ser Pro Gln Ala Trp Val 580 585 590cca atc ttt gag tcg aac aag cca aag gag caa aga aca atc aag gac 1824Pro Ile Phe Glu Ser Asn Lys Pro Lys Glu Gln Arg Thr Ile Lys Asp 595 600 605aag gac tat gtc gaa tgg aga gcc aag gct gcc aag ata cct ttc gac 1872Lys Asp Tyr Val Glu Trp Arg Ala Lys Ala Ala Lys Ile Pro Phe Asp 610 615 620acc tac ggt tct gcc tat ggg tcc gca cat caa atg tcc acc tgt cgt 1920Thr Tyr Gly Ser Ala Tyr Gly Ser Ala His Gln Met Ser Thr Cys Arg625 630 635 640atg tcc gga aag ggt cct aaa tac ggc gcc gtt gat acc gat ggt aga 1968Met Ser Gly Lys Gly Pro Lys Tyr Gly Ala Val Asp Thr Asp Gly Arg 645 650 655ttg ttt gaa tgt tcg aat gtc tat gtt gct gat gct agt gtt ttg cct 2016Leu Phe Glu Cys Ser Asn Val Tyr Val Ala Asp Ala Ser Val Leu Pro 660 665 670act gcc agc ggt gcc aac cca atg atc tcc acc atg acg ttt gct aga 2064Thr Ala Ser Gly Ala Asn Pro Met Ile Ser Thr Met Thr Phe Ala Arg 675 680 685cag att gcg tta ggt ttg gct gac tct ttg aag acc aaa ccc aag ttg 2112Gln Ile Ala Leu Gly Leu Ala Asp Ser Leu Lys Thr Lys Pro Lys Leu 690 695 700tag 211528704PRTCandida tropicalis 28Met Asn Thr Phe Leu Pro Asp Val Leu Glu Tyr Lys His Val Asp Thr1 5 10 15Leu Leu Leu Leu Cys Asp Gly Ile Ile His Glu Thr Thr Val Asp Gln 20 25 30Ile Arg Asp Ala Ile Ala Pro Asp Phe Pro Glu Asp Gln Tyr Glu Glu 35 40 45Tyr Leu Lys Thr Phe Thr Lys Pro Ser Glu Thr Pro Gly Phe Arg Glu 50 55 60Ala Val Tyr Asp Thr Ile Asn Ser Thr Pro Thr Glu Ala Val His Met65 70 75 80Cys Ile Val Leu Thr Thr Ala Leu Asp Ser Arg Ile Leu Ala Pro Thr 85 90 95Leu Thr Asn Ser Leu Thr Pro Ile Lys Asp Met Thr Leu Lys Glu Arg 100 105 110Glu Gln Leu Leu Ala Ala Trp Arg Asp Ser Pro Ile Ala Ala Lys Arg 115 120 125Arg Leu Phe Arg Leu Ile Ser Ser Leu Thr Leu Thr Thr Phe Thr Arg 130 135 140Leu Ala Ser Asp Leu His Leu Arg Ala Ile His Tyr Pro Gly Arg Asp145 150 155 160Leu Arg Glu Lys Ala Tyr Glu Thr Gln Val Val Asp Pro Phe Arg Tyr 165 170 175Leu Phe Met Glu Lys Pro Lys Phe Asp Gly Thr Glu Leu Tyr Leu Pro 180 185 190Asp Ile Asp Val Ile Ile Ile Gly Ser Gly Ala Gly Ala Gly Val Met 195 200 205Ala His Thr Leu Ala Asn Asp Gly Tyr Lys Thr Leu Val Leu Glu Lys 210 215 220Gly Lys Tyr Phe Ser Asn Ser Glu Leu Asn Phe Asn Asp Ala Asp Gly225 230 235 240Met Lys Glu Leu Tyr Gln Gly Lys Cys Ala Leu Thr Thr Thr Asn Gln 245 250 255Gln Met Phe Ile Leu Ala Gly Ser Thr Leu Gly Gly Gly Thr Thr Val 260 265 270Asn Trp Ser Ala Cys Leu Lys Thr Pro Phe Lys Val Arg Lys Glu Trp 275 280 285Tyr Asp Glu Phe Gly Leu Glu Phe Ala Ala Asp Glu Ala Tyr Asp Lys 290 295 300Ala Gln Asp Tyr Val Trp Lys Gln Met Gly Ala Ser Thr Glu Gly Ile305 310 315 320Thr His Ser Leu Ala Asn Ala Val Val Val Glu Gly Gly Lys Lys Leu 325 330 335Gly Tyr Lys Ser Lys Glu Ile Glu Gln Asn Asn Gly Gly His Pro Asp 340 345 350His Pro Cys Gly Phe Cys Tyr Leu Gly Cys Lys Tyr Gly Ile Lys Gln 355 360 365Gly Ser Val Asn Asn Trp Phe Arg Asp Ala Ala Ala His Gly Ser Lys 370 375 380Phe Met Gln Gln Val Arg Val Val Gln Ile Leu His Asn Lys Gly Val385 390 395 400Ala Tyr Gly Ile Leu Cys Glu Asp Val Glu Thr Gly Val Lys Phe Thr 405 410 415Ile Ser Gly Pro Lys Lys Phe Val Val Ser Ala Gly Ser Leu Asn Thr 420 425 430Pro Thr Val Leu Thr Asn Ser Gly Phe Lys Asn Lys His Ile Gly Lys 435 440 445Asn Leu Thr Leu His Pro Val Ser Thr Val Phe Gly Asp Phe Gly Arg 450 455 460Asp Val Gln Ala Asp His Phe His Lys Ser Ile Met Thr Ser Leu Cys465 470 475 480Tyr Glu Val Ala Asp Leu Asp Gly Lys Gly His Gly Cys Arg Ile Glu 485 490 495Thr Ile Leu Asn Ala Pro Phe Ile Gln Ala Ser Leu Leu Pro Trp Arg 500 505 510Gly Ser Asp Glu Val Arg Arg Asp Leu Leu Arg Tyr Asn Asn Met Val 515 520 525Ala Met Leu Leu Ile Thr Arg Asp Thr Thr Ser Gly Ser Val Ser Ala 530 535 540Asp Pro Lys Lys Pro Asp Ala Leu Ile Val Asp Tyr Asp Ile Asn Lys545 550 555 560Phe Asp Lys Asn Ala Ile Leu Gln Ala Phe Leu Ile Thr Ser Asp Met 565 570 575Leu Tyr Ile Glu Gly Ala Lys Arg Ile Leu Ser Pro Gln Ala Trp Val 580 585 590Pro Ile Phe Glu Ser Asn Lys Pro Lys Glu Gln Arg Thr Ile Lys Asp 595 600 605Lys Asp Tyr Val Glu Trp Arg Ala Lys Ala Ala Lys Ile Pro Phe Asp 610 615 620Thr Tyr Gly Ser Ala Tyr Gly Ser Ala His Gln Met Ser Thr Cys Arg625 630 635 640Met Ser Gly Lys Gly Pro Lys Tyr Gly Ala Val Asp Thr Asp Gly Arg 645 650 655Leu Phe Glu Cys Ser Asn Val Tyr Val Ala Asp Ala Ser Val Leu Pro 660 665 670Thr Ala Ser Gly Ala Asn Pro Met Ile Ser Thr Met Thr Phe Ala Arg 675 680 685Gln Ile Ala Leu Gly Leu Ala Asp Ser Leu Lys Thr Lys Pro Lys Leu 690 695 700291446DNACandida tropicalisCDS(1)..(1443) 29atg ata atg ttt gat atc gtc aaa tac acc tta att ggg tta ttt agc 48Met Ile Met Phe Asp Ile Val Lys Tyr Thr Leu Ile Gly Leu Phe Ser1 5 10 15tat ttg cta tat gta att ctc gat ata gta cta cca cca ttc aat ttt 96Tyr Leu Leu Tyr Val Ile Leu Asp Ile Val Leu Pro Pro Phe Asn Phe 20 25 30ccc aaa aat atc cca aca atc cca ttc tat gtt tcc ttc tta ggt gct 144Pro Lys Asn Ile Pro Thr Ile Pro Phe Tyr Val Ser Phe Leu Gly Ala 35 40 45tac act aac ttg gat cag agg gat att tac aat ttg tat ttg aga gag 192Tyr Thr Asn Leu Asp Gln Arg Asp Ile Tyr Asn Leu Tyr Leu Arg Glu 50 55 60aag ttg gaa aag tac ggg gca gta aag ata tat ttt gct tca aga tgg 240Lys Leu Glu Lys Tyr Gly Ala Val Lys Ile Tyr Phe Ala Ser Arg Trp65

70 75 80aac ata ctc att act agg cca gaa tat ctt ctt gaa atg ttt aga aat 288Asn Ile Leu Ile Thr Arg Pro Glu Tyr Leu Leu Glu Met Phe Arg Asn 85 90 95gaa gat gtg tac tca aaa cgg gga aac cac cta aaa atc cct ggt tca 336Glu Asp Val Tyr Ser Lys Arg Gly Asn His Leu Lys Ile Pro Gly Ser 100 105 110gtg atg gct aca tac act ggt gat aat atc att agt gct cat gga gaa 384Val Met Ala Thr Tyr Thr Gly Asp Asn Ile Ile Ser Ala His Gly Glu 115 120 125tta tgg aaa tta tat cga gaa gtt att gcc aaa agt att caa ttt ccc 432Leu Trp Lys Leu Tyr Arg Glu Val Ile Ala Lys Ser Ile Gln Phe Pro 130 135 140gat ttt gaa cca att acg aaa aat act aaa tca tta ctt gaa att att 480Asp Phe Glu Pro Ile Thr Lys Asn Thr Lys Ser Leu Leu Glu Ile Ile145 150 155 160gat ggt atg att gac agt gat aaa aat cat gca att att cca atc aca 528Asp Gly Met Ile Asp Ser Asp Lys Asn His Ala Ile Ile Pro Ile Thr 165 170 175gat tta ttt caa aaa tat tca tta gca aac gtt aca gaa tct ata ctt 576Asp Leu Phe Gln Lys Tyr Ser Leu Ala Asn Val Thr Glu Ser Ile Leu 180 185 190gga gta aat ttt aag gtt ctt gaa ggc gat caa tca atc atg cat caa 624Gly Val Asn Phe Lys Val Leu Glu Gly Asp Gln Ser Ile Met His Gln 195 200 205aaa ata aag tac gtc aag ctg caa ata ttc aaa cct ttt ttc ttg aac 672Lys Ile Lys Tyr Val Lys Leu Gln Ile Phe Lys Pro Phe Phe Leu Asn 210 215 220ttt cct tat ttt gat agc ttt cct att cca agt agg tta caa gca aga 720Phe Pro Tyr Phe Asp Ser Phe Pro Ile Pro Ser Arg Leu Gln Ala Arg225 230 235 240aag gaa gtt att aat ttt agg aat tgg tat ggt caa agt att att gac 768Lys Glu Val Ile Asn Phe Arg Asn Trp Tyr Gly Gln Ser Ile Ile Asp 245 250 255aag cat gat cca caa ctt cct aat agt gca gct aca aaa tta gtt gat 816Lys His Asp Pro Gln Leu Pro Asn Ser Ala Ala Thr Lys Leu Val Asp 260 265 270ggc ttg atg caa gaa aaa ctc act gaa aaa caa ttt ttg gat aat gcc 864Gly Leu Met Gln Glu Lys Leu Thr Glu Lys Gln Phe Leu Asp Asn Ala 275 280 285att att gtg atg att gct gga cat gaa aat cca ctt ttg tta atg tta 912Ile Ile Val Met Ile Ala Gly His Glu Asn Pro Leu Leu Leu Met Leu 290 295 300tcg ttg atg ttt gtt gct gca aaa tat cca aaa gtt cag gag gcc ata 960Ser Leu Met Phe Val Ala Ala Lys Tyr Pro Lys Val Gln Glu Ala Ile305 310 315 320cgt tca gaa ata gat cca aca aaa cct tat cta cac tct gtt att tat 1008Arg Ser Glu Ile Asp Pro Thr Lys Pro Tyr Leu His Ser Val Ile Tyr 325 330 335gaa act tta aga atg tat cca cca ttg gga tta atc att aat cgt tat 1056Glu Thr Leu Arg Met Tyr Pro Pro Leu Gly Leu Ile Ile Asn Arg Tyr 340 345 350acc acc aga cct act aaa cta ggc aac ata gta att ccc aaa ggt gtt 1104Thr Thr Arg Pro Thr Lys Leu Gly Asn Ile Val Ile Pro Lys Gly Val 355 360 365tac tgt ggc tat aat aat ttt ggt acg ggt aga gac aga aat gtt tgg 1152Tyr Cys Gly Tyr Asn Asn Phe Gly Thr Gly Arg Asp Arg Asn Val Trp 370 375 380gga cca gac tcg gat gag ttt aaa cca gag aga tgg gga agg gat aat 1200Gly Pro Asp Ser Asp Glu Phe Lys Pro Glu Arg Trp Gly Arg Asp Asn385 390 395 400att gaa gaa ata aat cgc aat tat gct aat gcc aaa aga tca gct gaa 1248Ile Glu Glu Ile Asn Arg Asn Tyr Ala Asn Ala Lys Arg Ser Ala Glu 405 410 415tta cct gcg ttt cat ggc aga aag aga gct tgt tta gga gaa aag tat 1296Leu Pro Ala Phe His Gly Arg Lys Arg Ala Cys Leu Gly Glu Lys Tyr 420 425 430gcc tta tat gaa gtt aaa gaa ttg cta act agt att tta gga cat tac 1344Ala Leu Tyr Glu Val Lys Glu Leu Leu Thr Ser Ile Leu Gly His Tyr 435 440 445aaa gtt act tta gat gca agt tgg aaa gag aaa ata acc cct gct gga 1392Lys Val Thr Leu Asp Ala Ser Trp Lys Glu Lys Ile Thr Pro Ala Gly 450 455 460cct att agt cca ttt ggt ttg aag gtg aaa ttt gaa aag ctt att gtt 1440Pro Ile Ser Pro Phe Gly Leu Lys Val Lys Phe Glu Lys Leu Ile Val465 470 475 480gca taa 1446Ala30481PRTCandida tropicalis 30Met Ile Met Phe Asp Ile Val Lys Tyr Thr Leu Ile Gly Leu Phe Ser1 5 10 15Tyr Leu Leu Tyr Val Ile Leu Asp Ile Val Leu Pro Pro Phe Asn Phe 20 25 30Pro Lys Asn Ile Pro Thr Ile Pro Phe Tyr Val Ser Phe Leu Gly Ala 35 40 45Tyr Thr Asn Leu Asp Gln Arg Asp Ile Tyr Asn Leu Tyr Leu Arg Glu 50 55 60Lys Leu Glu Lys Tyr Gly Ala Val Lys Ile Tyr Phe Ala Ser Arg Trp65 70 75 80Asn Ile Leu Ile Thr Arg Pro Glu Tyr Leu Leu Glu Met Phe Arg Asn 85 90 95Glu Asp Val Tyr Ser Lys Arg Gly Asn His Leu Lys Ile Pro Gly Ser 100 105 110Val Met Ala Thr Tyr Thr Gly Asp Asn Ile Ile Ser Ala His Gly Glu 115 120 125Leu Trp Lys Leu Tyr Arg Glu Val Ile Ala Lys Ser Ile Gln Phe Pro 130 135 140Asp Phe Glu Pro Ile Thr Lys Asn Thr Lys Ser Leu Leu Glu Ile Ile145 150 155 160Asp Gly Met Ile Asp Ser Asp Lys Asn His Ala Ile Ile Pro Ile Thr 165 170 175Asp Leu Phe Gln Lys Tyr Ser Leu Ala Asn Val Thr Glu Ser Ile Leu 180 185 190Gly Val Asn Phe Lys Val Leu Glu Gly Asp Gln Ser Ile Met His Gln 195 200 205Lys Ile Lys Tyr Val Lys Leu Gln Ile Phe Lys Pro Phe Phe Leu Asn 210 215 220Phe Pro Tyr Phe Asp Ser Phe Pro Ile Pro Ser Arg Leu Gln Ala Arg225 230 235 240Lys Glu Val Ile Asn Phe Arg Asn Trp Tyr Gly Gln Ser Ile Ile Asp 245 250 255Lys His Asp Pro Gln Leu Pro Asn Ser Ala Ala Thr Lys Leu Val Asp 260 265 270Gly Leu Met Gln Glu Lys Leu Thr Glu Lys Gln Phe Leu Asp Asn Ala 275 280 285Ile Ile Val Met Ile Ala Gly His Glu Asn Pro Leu Leu Leu Met Leu 290 295 300Ser Leu Met Phe Val Ala Ala Lys Tyr Pro Lys Val Gln Glu Ala Ile305 310 315 320Arg Ser Glu Ile Asp Pro Thr Lys Pro Tyr Leu His Ser Val Ile Tyr 325 330 335Glu Thr Leu Arg Met Tyr Pro Pro Leu Gly Leu Ile Ile Asn Arg Tyr 340 345 350Thr Thr Arg Pro Thr Lys Leu Gly Asn Ile Val Ile Pro Lys Gly Val 355 360 365Tyr Cys Gly Tyr Asn Asn Phe Gly Thr Gly Arg Asp Arg Asn Val Trp 370 375 380Gly Pro Asp Ser Asp Glu Phe Lys Pro Glu Arg Trp Gly Arg Asp Asn385 390 395 400Ile Glu Glu Ile Asn Arg Asn Tyr Ala Asn Ala Lys Arg Ser Ala Glu 405 410 415Leu Pro Ala Phe His Gly Arg Lys Arg Ala Cys Leu Gly Glu Lys Tyr 420 425 430Ala Leu Tyr Glu Val Lys Glu Leu Leu Thr Ser Ile Leu Gly His Tyr 435 440 445Lys Val Thr Leu Asp Ala Ser Trp Lys Glu Lys Ile Thr Pro Ala Gly 450 455 460Pro Ile Ser Pro Phe Gly Leu Lys Val Lys Phe Glu Lys Leu Ile Val465 470 475 480Ala311623DNACandida tropicalisCDS(1)..(1620) 31atg tcg tcg tct cca tct att gct caa gaa ttt ctc gca acc att act 48Met Ser Ser Ser Pro Ser Ile Ala Gln Glu Phe Leu Ala Thr Ile Thr1 5 10 15cca tat gtt gag tat tgt caa gag aat tat acc aaa tgg tac tac ttc 96Pro Tyr Val Glu Tyr Cys Gln Glu Asn Tyr Thr Lys Trp Tyr Tyr Phe 20 25 30att cca ttg gtg att ctt tcg ttg aat ctt atc tcc atg ctt cat aca 144Ile Pro Leu Val Ile Leu Ser Leu Asn Leu Ile Ser Met Leu His Thr 35 40 45aag tat ttg gaa cgt aaa ttt aag gct aaa ccg ctt gct gtc tat gtt 192Lys Tyr Leu Glu Arg Lys Phe Lys Ala Lys Pro Leu Ala Val Tyr Val 50 55 60caa gat tat acc ttt ggt ctt att act cca ctt gtt ttg atc tac tac 240Gln Asp Tyr Thr Phe Gly Leu Ile Thr Pro Leu Val Leu Ile Tyr Tyr65 70 75 80aag tct aaa ggt acc gtg atg caa ttt gcc tgt gat tta tgg gac aag 288Lys Ser Lys Gly Thr Val Met Gln Phe Ala Cys Asp Leu Trp Asp Lys 85 90 95aaa ctc att gtc agt gat cca aag gca aag act att ggt ctt aag att 336Lys Leu Ile Val Ser Asp Pro Lys Ala Lys Thr Ile Gly Leu Lys Ile 100 105 110tta ggt att cca ttg att gaa act aaa gac cca gag aat gtc aag gct 384Leu Gly Ile Pro Leu Ile Glu Thr Lys Asp Pro Glu Asn Val Lys Ala 115 120 125att tta gcc acc cag ttt aat gat ttt tct ttg gga act aga cat gat 432Ile Leu Ala Thr Gln Phe Asn Asp Phe Ser Leu Gly Thr Arg His Asp 130 135 140ttc ttg tat tca ttg tta gga gat ggt att ttc act tta gat ggt gct 480Phe Leu Tyr Ser Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Ala145 150 155 160ggc tgg aaa cac agc aga acc atg ttg aga cca caa ttc gct aga gag 528Gly Trp Lys His Ser Arg Thr Met Leu Arg Pro Gln Phe Ala Arg Glu 165 170 175caa gtt tcg cat gta aag ttg ttg gaa ccc cat atg caa gtt tta ttt 576Gln Val Ser His Val Lys Leu Leu Glu Pro His Met Gln Val Leu Phe 180 185 190aaa cac att aga aaa cat cat ggc caa act ttt gat atc caa gaa ttg 624Lys His Ile Arg Lys His His Gly Gln Thr Phe Asp Ile Gln Glu Leu 195 200 205ttt ttc aga tta act gtt gat tcg gct act gag ttt ttg ttt ggt gaa 672Phe Phe Arg Leu Thr Val Asp Ser Ala Thr Glu Phe Leu Phe Gly Glu 210 215 220tct gct gaa tct tta aga gac gag tct gtc ggt tta act cca aca acc 720Ser Ala Glu Ser Leu Arg Asp Glu Ser Val Gly Leu Thr Pro Thr Thr225 230 235 240aaa gat ttt gat ggt aga aat gag ttt gcc gat gcg ttc aac tat tct 768Lys Asp Phe Asp Gly Arg Asn Glu Phe Ala Asp Ala Phe Asn Tyr Ser 245 250 255caa act tac caa gcg tac aga ttc ttg ttg caa caa atg tat tgg att 816Gln Thr Tyr Gln Ala Tyr Arg Phe Leu Leu Gln Gln Met Tyr Trp Ile 260 265 270ttg aat ggt tct gaa ttt aga aaa tct att gct att gtc cac aag ttt 864Leu Asn Gly Ser Glu Phe Arg Lys Ser Ile Ala Ile Val His Lys Phe 275 280 285gct gac cac tat gtt caa aag gca ttg gaa tta act gat gaa gat ttg 912Ala Asp His Tyr Val Gln Lys Ala Leu Glu Leu Thr Asp Glu Asp Leu 290 295 300gaa aag aaa gaa ggc tat gta ttt tta ttc gag tta gcc aaa caa acc 960Glu Lys Lys Glu Gly Tyr Val Phe Leu Phe Glu Leu Ala Lys Gln Thr305 310 315 320aga gat cca aag gtt ttg aga gat caa ttg tta aat atc ttg gtt gct 1008Arg Asp Pro Lys Val Leu Arg Asp Gln Leu Leu Asn Ile Leu Val Ala 325 330 335ggt aga gat acc aca gct ggc ttg ttg tcg ttt ctt ttc ttt gaa ttg 1056Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Leu Phe Phe Glu Leu 340 345 350tct aga aac cca gaa ata ttt gca aaa ttg aga gaa gaa atc gaa aac 1104Ser Arg Asn Pro Glu Ile Phe Ala Lys Leu Arg Glu Glu Ile Glu Asn 355 360 365aag ttt ggt ctt gga caa gat gct cgt gtt gaa gag att tct ttt gaa 1152Lys Phe Gly Leu Gly Gln Asp Ala Arg Val Glu Glu Ile Ser Phe Glu 370 375 380aca ttg aaa tct tgt gaa tac ttg aag gct gtt atc aat gaa act ttg 1200Thr Leu Lys Ser Cys Glu Tyr Leu Lys Ala Val Ile Asn Glu Thr Leu385 390 395 400aga att tat cct tcc gtc cca cat aat ttt aga gtt gct act aga aac 1248Arg Ile Tyr Pro Ser Val Pro His Asn Phe Arg Val Ala Thr Arg Asn 405 410 415aca act tta cca aga ggt ggt ggt gaa ggt ggt tta tcc cca att gct 1296Thr Thr Leu Pro Arg Gly Gly Gly Glu Gly Gly Leu Ser Pro Ile Ala 420 425 430att aag aag ggc caa gtt gtt atg tac acg att ctt gct act cac aga 1344Ile Lys Lys Gly Gln Val Val Met Tyr Thr Ile Leu Ala Thr His Arg 435 440 445gat aaa gac att tat ggt gaa gat gct tat gtt ttc agg cca gaa aga 1392Asp Lys Asp Ile Tyr Gly Glu Asp Ala Tyr Val Phe Arg Pro Glu Arg 450 455 460tgg ttt gaa cct gaa acc aga aaa ttg ggc tgg gca tat gtt cca ttc 1440Trp Phe Glu Pro Glu Thr Arg Lys Leu Gly Trp Ala Tyr Val Pro Phe465 470 475 480aat ggc ggt cca aga att tgt ttg ggt caa cag ttt gct tta act gaa 1488Asn Gly Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe Ala Leu Thr Glu 485 490 495gca tca tat gtc act gtt aga ttg ctt caa gaa ttt ggt aac ttg aaa 1536Ala Ser Tyr Val Thr Val Arg Leu Leu Gln Glu Phe Gly Asn Leu Lys 500 505 510caa gat cca aat act gaa tat cca cca aaa tta caa aac aca ttg act 1584Gln Asp Pro Asn Thr Glu Tyr Pro Pro Lys Leu Gln Asn Thr Leu Thr 515 520 525ttg tct ctt ttt gaa ggt gct gaa gta caa atg tat taa 1623Leu Ser Leu Phe Glu Gly Ala Glu Val Gln Met Tyr 530 535 54032540PRTCandida tropicalis 32Met Ser Ser Ser Pro Ser Ile Ala Gln Glu Phe Leu Ala Thr Ile Thr1 5 10 15Pro Tyr Val Glu Tyr Cys Gln Glu Asn Tyr Thr Lys Trp Tyr Tyr Phe 20 25 30Ile Pro Leu Val Ile Leu Ser Leu Asn Leu Ile Ser Met Leu His Thr 35 40 45Lys Tyr Leu Glu Arg Lys Phe Lys Ala Lys Pro Leu Ala Val Tyr Val 50 55 60Gln Asp Tyr Thr Phe Gly Leu Ile Thr Pro Leu Val Leu Ile Tyr Tyr65 70 75 80Lys Ser Lys Gly Thr Val Met Gln Phe Ala Cys Asp Leu Trp Asp Lys 85 90 95Lys Leu Ile Val Ser Asp Pro Lys Ala Lys Thr Ile Gly Leu Lys Ile 100 105 110Leu Gly Ile Pro Leu Ile Glu Thr Lys Asp Pro Glu Asn Val Lys Ala 115 120 125Ile Leu Ala Thr Gln Phe Asn Asp Phe Ser Leu Gly Thr Arg His Asp 130 135 140Phe Leu Tyr Ser Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Ala145 150 155 160Gly Trp Lys His Ser Arg Thr Met Leu Arg Pro Gln Phe Ala Arg Glu 165 170 175Gln Val Ser His Val Lys Leu Leu Glu Pro His Met Gln Val Leu Phe 180 185 190Lys His Ile Arg Lys His His Gly Gln Thr Phe Asp Ile Gln Glu Leu 195 200 205Phe Phe Arg Leu Thr Val Asp Ser Ala Thr Glu Phe Leu Phe Gly Glu 210 215 220Ser Ala Glu Ser Leu Arg Asp Glu Ser Val Gly Leu Thr Pro Thr Thr225 230 235 240Lys Asp Phe Asp Gly Arg Asn Glu Phe Ala Asp Ala Phe Asn Tyr Ser 245 250 255Gln Thr Tyr Gln Ala Tyr Arg Phe Leu Leu Gln Gln Met Tyr Trp Ile 260 265 270Leu Asn Gly Ser Glu Phe Arg Lys Ser Ile Ala Ile Val His Lys Phe 275 280 285Ala Asp His Tyr Val Gln Lys Ala Leu Glu Leu Thr Asp Glu Asp Leu 290 295 300Glu Lys Lys Glu Gly Tyr Val Phe Leu Phe Glu Leu Ala Lys Gln Thr305 310 315 320Arg Asp Pro Lys Val Leu Arg Asp Gln Leu Leu Asn Ile Leu Val Ala 325 330 335Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Leu Phe Phe Glu Leu 340 345 350Ser Arg Asn Pro Glu Ile Phe Ala Lys Leu Arg Glu Glu Ile Glu Asn 355 360 365Lys Phe Gly Leu Gly Gln Asp Ala Arg Val Glu Glu Ile Ser Phe Glu 370 375 380Thr Leu Lys Ser Cys Glu Tyr Leu Lys Ala Val Ile Asn Glu Thr Leu385 390 395 400Arg Ile Tyr Pro Ser Val Pro His Asn Phe Arg Val Ala Thr Arg Asn 405 410 415Thr Thr Leu Pro Arg Gly Gly Gly Glu Gly Gly Leu Ser Pro Ile Ala 420 425 430Ile Lys Lys Gly Gln Val Val Met Tyr Thr Ile Leu Ala Thr His Arg 435 440 445Asp Lys

Asp Ile Tyr Gly Glu Asp Ala Tyr Val Phe Arg Pro Glu Arg 450 455 460Trp Phe Glu Pro Glu Thr Arg Lys Leu Gly Trp Ala Tyr Val Pro Phe465 470 475 480Asn Gly Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe Ala Leu Thr Glu 485 490 495Ala Ser Tyr Val Thr Val Arg Leu Leu Gln Glu Phe Gly Asn Leu Lys 500 505 510Gln Asp Pro Asn Thr Glu Tyr Pro Pro Lys Leu Gln Asn Thr Leu Thr 515 520 525Leu Ser Leu Phe Glu Gly Ala Glu Val Gln Met Tyr 530 535 540331569DNACandida tropicalisCDS(1)..(1566) 33atg agt att caa gat att gtt gaa act tac agt acc aaa tgg tac gtt 48Met Ser Ile Gln Asp Ile Val Glu Thr Tyr Ser Thr Lys Trp Tyr Val1 5 10 15gtt gta ctg gtt gct ttg att gta tac aag gtt ttt gat ttc ttt tat 96Val Val Leu Val Ala Leu Ile Val Tyr Lys Val Phe Asp Phe Phe Tyr 20 25 30gct aga tat ttg atg tat aag ctt ggt gct aag cca ttc ctt caa agt 144Ala Arg Tyr Leu Met Tyr Lys Leu Gly Ala Lys Pro Phe Leu Gln Ser 35 40 45caa acc gac ggt tat ctt ggt ttc aga gtt cca ttt gaa ttg atg gga 192Gln Thr Asp Gly Tyr Leu Gly Phe Arg Val Pro Phe Glu Leu Met Gly 50 55 60aag aag agt gaa ggt aca ctt ata gac ttt aca tat caa cgt act ttg 240Lys Lys Ser Glu Gly Thr Leu Ile Asp Phe Thr Tyr Gln Arg Thr Leu65 70 75 80gag ctt gac aat ccc gat att cca aca ttt aca ttc cca ata ttt tct 288Glu Leu Asp Asn Pro Asp Ile Pro Thr Phe Thr Phe Pro Ile Phe Ser 85 90 95gtt ctg att atc tca act ctt gaa cca gac aac atc aaa gct att ttg 336Val Leu Ile Ile Ser Thr Leu Glu Pro Asp Asn Ile Lys Ala Ile Leu 100 105 110gcc aca caa ttc aat gac ttt tca tta ggt aca aga cat tca cat ttt 384Ala Thr Gln Phe Asn Asp Phe Ser Leu Gly Thr Arg His Ser His Phe 115 120 125gct cct tta tta ggt gac ggt att ttc act tta gat ggt gct ggt tgg 432Ala Pro Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Ala Gly Trp 130 135 140aaa cat agt aga tct atg ttg aga cca caa ttt gca aga gaa cag gtt 480Lys His Ser Arg Ser Met Leu Arg Pro Gln Phe Ala Arg Glu Gln Val145 150 155 160tct cac gtt aag ttg ttg gaa cca cat atg caa gtt ttt ttc aaa cat 528Ser His Val Lys Leu Leu Glu Pro His Met Gln Val Phe Phe Lys His 165 170 175att aga aaa cat cat ggc caa acc ttt gat ata caa gaa ttg ttt ttc 576Ile Arg Lys His His Gly Gln Thr Phe Asp Ile Gln Glu Leu Phe Phe 180 185 190aga tta act gtt gat tct gcc act gag ttt ttg ttt ggt gaa tct gtt 624Arg Leu Thr Val Asp Ser Ala Thr Glu Phe Leu Phe Gly Glu Ser Val 195 200 205gag tct tta aga gac gag tcc atc ggt atg tta aac gat gct ctt gat 672Glu Ser Leu Arg Asp Glu Ser Ile Gly Met Leu Asn Asp Ala Leu Asp 210 215 220ttt gat ggt aag gct gga ttt gct gat gcc ttt aac tat tct caa aac 720Phe Asp Gly Lys Ala Gly Phe Ala Asp Ala Phe Asn Tyr Ser Gln Asn225 230 235 240tat ttg gct tct cga gct ctt atg caa caa atg tac tgg att ttg aac 768Tyr Leu Ala Ser Arg Ala Leu Met Gln Gln Met Tyr Trp Ile Leu Asn 245 250 255gga aaa aag ttt aaa gaa tgt aat gcc aag gtt cac aag ttt gct gat 816Gly Lys Lys Phe Lys Glu Cys Asn Ala Lys Val His Lys Phe Ala Asp 260 265 270tat tat gtt gaa aaa gca ttg gaa tta act ccg gac caa ttg gaa aaa 864Tyr Tyr Val Glu Lys Ala Leu Glu Leu Thr Pro Asp Gln Leu Glu Lys 275 280 285caa gat ggg tat gtt ttc ttg tat gaa ttg gta aaa caa acc aga gac 912Gln Asp Gly Tyr Val Phe Leu Tyr Glu Leu Val Lys Gln Thr Arg Asp 290 295 300aga caa gtc ttg aga gat cag tta ttg aat ata tta gtt gct ggt aga 960Arg Gln Val Leu Arg Asp Gln Leu Leu Asn Ile Leu Val Ala Gly Arg305 310 315 320gat acc act gct ggt tta tta tca ttt gtg ttt ttt gaa ttg gca aga 1008Asp Thr Thr Ala Gly Leu Leu Ser Phe Val Phe Phe Glu Leu Ala Arg 325 330 335act cca aga gta gca aat aaa tta aga gaa gaa atc gaa gac aaa ttt 1056Thr Pro Arg Val Ala Asn Lys Leu Arg Glu Glu Ile Glu Asp Lys Phe 340 345 350ggc ctt gga caa gat gct cgt gtt gaa gaa att tcc ttt gaa tct tta 1104Gly Leu Gly Gln Asp Ala Arg Val Glu Glu Ile Ser Phe Glu Ser Leu 355 360 365aaa tca tgt gaa tat ttg aag gca gtg ctt aat gaa tgt tta aga tta 1152Lys Ser Cys Glu Tyr Leu Lys Ala Val Leu Asn Glu Cys Leu Arg Leu 370 375 380tac cca tct gtt cca caa aac ttt aga gtt gct acc aga aat acc aca 1200Tyr Pro Ser Val Pro Gln Asn Phe Arg Val Ala Thr Arg Asn Thr Thr385 390 395 400tta cca aga ggt ggt ggc aag gat ggt tta tca cca gta tta gtt aga 1248Leu Pro Arg Gly Gly Gly Lys Asp Gly Leu Ser Pro Val Leu Val Arg 405 410 415aag ggt caa act gtg atg tac agt gtg tat gct gcc cat aga aac aaa 1296Lys Gly Gln Thr Val Met Tyr Ser Val Tyr Ala Ala His Arg Asn Lys 420 425 430caa att tat ggt gaa gac gca ctt gaa ttc agg cca gaa aga tgg ttt 1344Gln Ile Tyr Gly Glu Asp Ala Leu Glu Phe Arg Pro Glu Arg Trp Phe 435 440 445gaa cca gag aca aag aaa ttg ggc tgg gcc ttc tta cct ttt aat ggt 1392Glu Pro Glu Thr Lys Lys Leu Gly Trp Ala Phe Leu Pro Phe Asn Gly 450 455 460ggt cca aga att tgt ttg ggt caa caa ttt gct ttg act gaa gct tct 1440Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe Ala Leu Thr Glu Ala Ser465 470 475 480tat gtt act gtt aga tta ctt caa gag ttt agc cac ttg aca atg gat 1488Tyr Val Thr Val Arg Leu Leu Gln Glu Phe Ser His Leu Thr Met Asp 485 490 495cca aac act gaa tac ccg cca aag aaa atg tcc cat ttg aca atg tct 1536Pro Asn Thr Glu Tyr Pro Pro Lys Lys Met Ser His Leu Thr Met Ser 500 505 510cta ttt gat ggt gcc aac att caa atg tat tag 1569Leu Phe Asp Gly Ala Asn Ile Gln Met Tyr 515 52034522PRTCandida tropicalis 34Met Ser Ile Gln Asp Ile Val Glu Thr Tyr Ser Thr Lys Trp Tyr Val1 5 10 15Val Val Leu Val Ala Leu Ile Val Tyr Lys Val Phe Asp Phe Phe Tyr 20 25 30Ala Arg Tyr Leu Met Tyr Lys Leu Gly Ala Lys Pro Phe Leu Gln Ser 35 40 45Gln Thr Asp Gly Tyr Leu Gly Phe Arg Val Pro Phe Glu Leu Met Gly 50 55 60Lys Lys Ser Glu Gly Thr Leu Ile Asp Phe Thr Tyr Gln Arg Thr Leu65 70 75 80Glu Leu Asp Asn Pro Asp Ile Pro Thr Phe Thr Phe Pro Ile Phe Ser 85 90 95Val Leu Ile Ile Ser Thr Leu Glu Pro Asp Asn Ile Lys Ala Ile Leu 100 105 110Ala Thr Gln Phe Asn Asp Phe Ser Leu Gly Thr Arg His Ser His Phe 115 120 125Ala Pro Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Ala Gly Trp 130 135 140Lys His Ser Arg Ser Met Leu Arg Pro Gln Phe Ala Arg Glu Gln Val145 150 155 160Ser His Val Lys Leu Leu Glu Pro His Met Gln Val Phe Phe Lys His 165 170 175Ile Arg Lys His His Gly Gln Thr Phe Asp Ile Gln Glu Leu Phe Phe 180 185 190Arg Leu Thr Val Asp Ser Ala Thr Glu Phe Leu Phe Gly Glu Ser Val 195 200 205Glu Ser Leu Arg Asp Glu Ser Ile Gly Met Leu Asn Asp Ala Leu Asp 210 215 220Phe Asp Gly Lys Ala Gly Phe Ala Asp Ala Phe Asn Tyr Ser Gln Asn225 230 235 240Tyr Leu Ala Ser Arg Ala Leu Met Gln Gln Met Tyr Trp Ile Leu Asn 245 250 255Gly Lys Lys Phe Lys Glu Cys Asn Ala Lys Val His Lys Phe Ala Asp 260 265 270Tyr Tyr Val Glu Lys Ala Leu Glu Leu Thr Pro Asp Gln Leu Glu Lys 275 280 285Gln Asp Gly Tyr Val Phe Leu Tyr Glu Leu Val Lys Gln Thr Arg Asp 290 295 300Arg Gln Val Leu Arg Asp Gln Leu Leu Asn Ile Leu Val Ala Gly Arg305 310 315 320Asp Thr Thr Ala Gly Leu Leu Ser Phe Val Phe Phe Glu Leu Ala Arg 325 330 335Thr Pro Arg Val Ala Asn Lys Leu Arg Glu Glu Ile Glu Asp Lys Phe 340 345 350Gly Leu Gly Gln Asp Ala Arg Val Glu Glu Ile Ser Phe Glu Ser Leu 355 360 365Lys Ser Cys Glu Tyr Leu Lys Ala Val Leu Asn Glu Cys Leu Arg Leu 370 375 380Tyr Pro Ser Val Pro Gln Asn Phe Arg Val Ala Thr Arg Asn Thr Thr385 390 395 400Leu Pro Arg Gly Gly Gly Lys Asp Gly Leu Ser Pro Val Leu Val Arg 405 410 415Lys Gly Gln Thr Val Met Tyr Ser Val Tyr Ala Ala His Arg Asn Lys 420 425 430Gln Ile Tyr Gly Glu Asp Ala Leu Glu Phe Arg Pro Glu Arg Trp Phe 435 440 445Glu Pro Glu Thr Lys Lys Leu Gly Trp Ala Phe Leu Pro Phe Asn Gly 450 455 460Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe Ala Leu Thr Glu Ala Ser465 470 475 480Tyr Val Thr Val Arg Leu Leu Gln Glu Phe Ser His Leu Thr Met Asp 485 490 495Pro Asn Thr Glu Tyr Pro Pro Lys Lys Met Ser His Leu Thr Met Ser 500 505 510Leu Phe Asp Gly Ala Asn Ile Gln Met Tyr 515 520351572DNACandida tropicalisCDS(1)..(1569) 35atg gcc aca caa gaa att att gat tct gca ctt ccg tac ttg aca aag 48Met Ala Thr Gln Glu Ile Ile Asp Ser Ala Leu Pro Tyr Leu Thr Lys1 5 10 15tgg tat act gtt atc act tta gca gct ttg gtt ttc tta att tca tct 96Trp Tyr Thr Val Ile Thr Leu Ala Ala Leu Val Phe Leu Ile Ser Ser 20 25 30aat att aaa aat tac gtc aag gct aag aag ttg aaa tgc aga gat cct 144Asn Ile Lys Asn Tyr Val Lys Ala Lys Lys Leu Lys Cys Arg Asp Pro 35 40 45cca tat ttc aaa gga gcc ggt tgg aca ggt att agt cca tta att gaa 192Pro Tyr Phe Lys Gly Ala Gly Trp Thr Gly Ile Ser Pro Leu Ile Glu 50 55 60att att aaa gtt aaa ggt aat ggt aga ttg gca gat ttt gcc gat aaa 240Ile Ile Lys Val Lys Gly Asn Gly Arg Leu Ala Asp Phe Ala Asp Lys65 70 75 80aca ttc gac gac tat cca aac cat act ttt tac atg tct att att ggt 288Thr Phe Asp Asp Tyr Pro Asn His Thr Phe Tyr Met Ser Ile Ile Gly 85 90 95gct ttg aaa atc gtc ttg act gtt gat cca gaa aat att aaa gct gtt 336Ala Leu Lys Ile Val Leu Thr Val Asp Pro Glu Asn Ile Lys Ala Val 100 105 110ttg gct act caa ttt act gat ttc tcc tta ggt acc aga cat gct cat 384Leu Ala Thr Gln Phe Thr Asp Phe Ser Leu Gly Thr Arg His Ala His 115 120 125ttc tat cca tta tta ggt gat ggt att ttt act ttg gat ggt gaa ggt 432Phe Tyr Pro Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Glu Gly 130 135 140tgg aaa cat agt aga gct atg ttg aga cca caa ttt gct aga gat caa 480Trp Lys His Ser Arg Ala Met Leu Arg Pro Gln Phe Ala Arg Asp Gln145 150 155 160att ggt cat gtt aaa gct ttg gaa cca cat att caa atc ttg gcc aaa 528Ile Gly His Val Lys Ala Leu Glu Pro His Ile Gln Ile Leu Ala Lys 165 170 175caa att aaa ttg aat aaa ggt aaa act ttt gat att caa gaa ttg ttt 576Gln Ile Lys Leu Asn Lys Gly Lys Thr Phe Asp Ile Gln Glu Leu Phe 180 185 190ttc aga ttt act gtt gat act gct act gaa ttc ttg ttt ggt gaa tct 624Phe Arg Phe Thr Val Asp Thr Ala Thr Glu Phe Leu Phe Gly Glu Ser 195 200 205gtt cac tct ttg tat gat gaa aaa tta ggt att cct act cca aat gaa 672Val His Ser Leu Tyr Asp Glu Lys Leu Gly Ile Pro Thr Pro Asn Glu 210 215 220att cca ggt aga gat aat ttt gca act gct ttt aac act tct caa cat 720Ile Pro Gly Arg Asp Asn Phe Ala Thr Ala Phe Asn Thr Ser Gln His225 230 235 240tat ttg gct acc aga aca tac tcc caa act ttc tac ttt tta act aac 768Tyr Leu Ala Thr Arg Thr Tyr Ser Gln Thr Phe Tyr Phe Leu Thr Asn 245 250 255cct aag gaa ttt aga gac tgt aat gct aaa gtt cat tac ttg gct aaa 816Pro Lys Glu Phe Arg Asp Cys Asn Ala Lys Val His Tyr Leu Ala Lys 260 265 270tat ttt gtc aat aaa gct ttg aat ttc act ccg gaa gaa att gaa gaa 864Tyr Phe Val Asn Lys Ala Leu Asn Phe Thr Pro Glu Glu Ile Glu Glu 275 280 285aag tcc aaa tct ggt tat gtt ttc ttg tat gaa ttg gtt aaa caa acc 912Lys Ser Lys Ser Gly Tyr Val Phe Leu Tyr Glu Leu Val Lys Gln Thr 290 295 300aga gat cca aaa gtt tta caa gat caa tta ttg aac att atg gtt gcc 960Arg Asp Pro Lys Val Leu Gln Asp Gln Leu Leu Asn Ile Met Val Ala305 310 315 320ggt aga gat acc act gct ggt tta tta tca ttt gca atg ttt gaa tta 1008Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Ala Met Phe Glu Leu 325 330 335gct aga cat cca gaa att tgg tct aaa tta aga gaa gaa att gaa gtt 1056Ala Arg His Pro Glu Ile Trp Ser Lys Leu Arg Glu Glu Ile Glu Val 340 345 350aac ttt ggt gtt ggt gaa gaa tct cgt gtt gaa gaa att act ttt gaa 1104Asn Phe Gly Val Gly Glu Glu Ser Arg Val Glu Glu Ile Thr Phe Glu 355 360 365tct ttg aag aga tgt gaa tac ttg aaa gct att ctt aat gaa act ttg 1152Ser Leu Lys Arg Cys Glu Tyr Leu Lys Ala Ile Leu Asn Glu Thr Leu 370 375 380cgt atg tat cct tct gtt cca gtc aat tcc aga aca gcc act aga gat 1200Arg Met Tyr Pro Ser Val Pro Val Asn Ser Arg Thr Ala Thr Arg Asp385 390 395 400acc aca tta cca aga ggt ggt ggt cca aat ggt act gat cca att ttt 1248Thr Thr Leu Pro Arg Gly Gly Gly Pro Asn Gly Thr Asp Pro Ile Phe 405 410 415att cca aag ggt tcc act gtt gct tat att gtt tac aaa act cat cgt 1296Ile Pro Lys Gly Ser Thr Val Ala Tyr Ile Val Tyr Lys Thr His Arg 420 425 430tta gaa gaa tat tat ggt aaa gat gct gat gat ttc aga cca gaa aga 1344Leu Glu Glu Tyr Tyr Gly Lys Asp Ala Asp Asp Phe Arg Pro Glu Arg 435 440 445tgg ttt gaa cca tca act aaa aag tta ggt tgg gct tat gtt cca ttt 1392Trp Phe Glu Pro Ser Thr Lys Lys Leu Gly Trp Ala Tyr Val Pro Phe 450 455 460aat ggt ggt cca aga att tgt tta ggc caa caa ttt gct tta act gaa 1440Asn Gly Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe Ala Leu Thr Glu465 470 475 480gct tct tat gtt att acc aga ttg gta caa atg ttt gaa act gtt tct 1488Ala Ser Tyr Val Ile Thr Arg Leu Val Gln Met Phe Glu Thr Val Ser 485 490 495tct tcc cca gat gtt gaa tac cct cca cca aaa tgt att cat ttg act 1536Ser Ser Pro Asp Val Glu Tyr Pro Pro Pro Lys Cys Ile His Leu Thr 500 505 510atg agt cat gat gat ggt gtt ttc gtt aaa atg taa 1572Met Ser His Asp Asp Gly Val Phe Val Lys Met 515 52036523PRTCandida tropicalis 36Met Ala Thr Gln Glu Ile Ile Asp Ser Ala Leu Pro Tyr Leu Thr Lys1 5 10 15Trp Tyr Thr Val Ile Thr Leu Ala Ala Leu Val Phe Leu Ile Ser Ser 20 25 30Asn Ile Lys Asn Tyr Val Lys Ala Lys Lys Leu Lys Cys Arg Asp Pro 35 40 45Pro Tyr Phe Lys Gly Ala Gly Trp Thr Gly Ile Ser Pro Leu Ile Glu 50 55 60Ile Ile Lys Val Lys Gly Asn Gly Arg Leu Ala Asp Phe Ala Asp Lys65 70 75 80Thr Phe Asp Asp Tyr Pro Asn His Thr Phe Tyr Met Ser Ile Ile Gly 85 90 95Ala Leu Lys Ile Val Leu Thr Val Asp Pro Glu Asn Ile Lys Ala Val 100 105 110Leu Ala Thr Gln Phe Thr Asp Phe Ser Leu Gly Thr Arg His Ala His 115 120 125Phe Tyr Pro Leu Leu Gly Asp Gly Ile Phe Thr Leu Asp Gly Glu Gly 130 135 140Trp Lys His Ser Arg Ala Met Leu Arg Pro Gln Phe Ala Arg Asp Gln145 150 155 160Ile Gly

His Val Lys Ala Leu Glu Pro His Ile Gln Ile Leu Ala Lys 165 170 175Gln Ile Lys Leu Asn Lys Gly Lys Thr Phe Asp Ile Gln Glu Leu Phe 180 185 190Phe Arg Phe Thr Val Asp Thr Ala Thr Glu Phe Leu Phe Gly Glu Ser 195 200 205Val His Ser Leu Tyr Asp Glu Lys Leu Gly Ile Pro Thr Pro Asn Glu 210 215 220Ile Pro Gly Arg Asp Asn Phe Ala Thr Ala Phe Asn Thr Ser Gln His225 230 235 240Tyr Leu Ala Thr Arg Thr Tyr Ser Gln Thr Phe Tyr Phe Leu Thr Asn 245 250 255Pro Lys Glu Phe Arg Asp Cys Asn Ala Lys Val His Tyr Leu Ala Lys 260 265 270Tyr Phe Val Asn Lys Ala Leu Asn Phe Thr Pro Glu Glu Ile Glu Glu 275 280 285Lys Ser Lys Ser Gly Tyr Val Phe Leu Tyr Glu Leu Val Lys Gln Thr 290 295 300Arg Asp Pro Lys Val Leu Gln Asp Gln Leu Leu Asn Ile Met Val Ala305 310 315 320Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Ala Met Phe Glu Leu 325 330 335Ala Arg His Pro Glu Ile Trp Ser Lys Leu Arg Glu Glu Ile Glu Val 340 345 350Asn Phe Gly Val Gly Glu Glu Ser Arg Val Glu Glu Ile Thr Phe Glu 355 360 365Ser Leu Lys Arg Cys Glu Tyr Leu Lys Ala Ile Leu Asn Glu Thr Leu 370 375 380Arg Met Tyr Pro Ser Val Pro Val Asn Ser Arg Thr Ala Thr Arg Asp385 390 395 400Thr Thr Leu Pro Arg Gly Gly Gly Pro Asn Gly Thr Asp Pro Ile Phe 405 410 415Ile Pro Lys Gly Ser Thr Val Ala Tyr Ile Val Tyr Lys Thr His Arg 420 425 430Leu Glu Glu Tyr Tyr Gly Lys Asp Ala Asp Asp Phe Arg Pro Glu Arg 435 440 445Trp Phe Glu Pro Ser Thr Lys Lys Leu Gly Trp Ala Tyr Val Pro Phe 450 455 460Asn Gly Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe Ala Leu Thr Glu465 470 475 480Ala Ser Tyr Val Ile Thr Arg Leu Val Gln Met Phe Glu Thr Val Ser 485 490 495Ser Ser Pro Asp Val Glu Tyr Pro Pro Pro Lys Cys Ile His Leu Thr 500 505 510Met Ser His Asp Asp Gly Val Phe Val Lys Met 515 520371512DNACandida tropicalisCDS(1)..(1509) 37atg tca tta aca gaa aca act gct acg ttt att tac aat tat tgg tac 48Met Ser Leu Thr Glu Thr Thr Ala Thr Phe Ile Tyr Asn Tyr Trp Tyr1 5 10 15atc ata ttc att ata ttc tac aca acc agc aaa atc atc aag tat cat 96Ile Ile Phe Ile Ile Phe Tyr Thr Thr Ser Lys Ile Ile Lys Tyr His 20 25 30cac acg aca tat ctt atg ata aag ttc aaa gct tct ccg cct ttg aat 144His Thr Thr Tyr Leu Met Ile Lys Phe Lys Ala Ser Pro Pro Leu Asn 35 40 45tac ata aat aaa ggt ttt ttt gga att cag gcg acg ttc acg gaa ttg 192Tyr Ile Asn Lys Gly Phe Phe Gly Ile Gln Ala Thr Phe Thr Glu Leu 50 55 60aaa cat ctt ata tgt cac aca tcg att gat tac gcc atc gat caa ttc 240Lys His Leu Ile Cys His Thr Ser Ile Asp Tyr Ala Ile Asp Gln Phe65 70 75 80aat aac gtc cca ttc cca cat gtt cat act ttt gta acc aaa gtt ctt 288Asn Asn Val Pro Phe Pro His Val His Thr Phe Val Thr Lys Val Leu 85 90 95ggt aat gag tta atc atg aca aaa gat cct gaa aat att aaa gtt tta 336Gly Asn Glu Leu Ile Met Thr Lys Asp Pro Glu Asn Ile Lys Val Leu 100 105 110ttg agt tcc agt ttt gat aag ttt gat tat gga aca cgt tca agt gcc 384Leu Ser Ser Ser Phe Asp Lys Phe Asp Tyr Gly Thr Arg Ser Ser Ala 115 120 125gtg caa cca tct tta gga atg ggg ata ttc act ctt gaa gga gaa aat 432Val Gln Pro Ser Leu Gly Met Gly Ile Phe Thr Leu Glu Gly Glu Asn 130 135 140tgg aaa gca aca aga agt gtt tta agg aac atg ttt gat aga aaa tca 480Trp Lys Ala Thr Arg Ser Val Leu Arg Asn Met Phe Asp Arg Lys Ser145 150 155 160att gac aag gta cat gat ttt gaa cca cat ttc aaa acc ctt cag aaa 528Ile Asp Lys Val His Asp Phe Glu Pro His Phe Lys Thr Leu Gln Lys 165 170 175aga ata gat ggg aag gtt gga tat ttt gac atc caa cag gag ttt tta 576Arg Ile Asp Gly Lys Val Gly Tyr Phe Asp Ile Gln Gln Glu Phe Leu 180 185 190aaa tta gga ttg gaa ttg agt att gag ttt att ttt ggt caa gtt gta 624Lys Leu Gly Leu Glu Leu Ser Ile Glu Phe Ile Phe Gly Gln Val Val 195 200 205tcg gaa gat gtc cca cat tat gat gat ttt acc cag gct tgg gat aga 672Ser Glu Asp Val Pro His Tyr Asp Asp Phe Thr Gln Ala Trp Asp Arg 210 215 220tgt caa gac tat atg atg cta aga tta ttg ttg ggg gat ttt tat tgg 720Cys Gln Asp Tyr Met Met Leu Arg Leu Leu Leu Gly Asp Phe Tyr Trp225 230 235 240ata gct aat gac tgg aga tat aaa cag tcc aat caa att gtg caa gct 768Ile Ala Asn Asp Trp Arg Tyr Lys Gln Ser Asn Gln Ile Val Gln Ala 245 250 255ttt tgt gat tat ttg gtg caa aaa tca ctt gaa aat aca tgc aac gac 816Phe Cys Asp Tyr Leu Val Gln Lys Ser Leu Glu Asn Thr Cys Asn Asp 260 265 270aaa ttt gtc ttt gta cag gaa ctt gca aaa cac acg acc aac aaa acg 864Lys Phe Val Phe Val Gln Glu Leu Ala Lys His Thr Thr Asn Lys Thr 275 280 285ttc att aga gat caa gca tta agc ttg att atg gct tca aga gac aca 912Phe Ile Arg Asp Gln Ala Leu Ser Leu Ile Met Ala Ser Arg Asp Thr 290 295 300act gct gag ttg atg gca ttc acc ata cta gaa tta tcc aga aat ccc 960Thr Ala Glu Leu Met Ala Phe Thr Ile Leu Glu Leu Ser Arg Asn Pro305 310 315 320act atc tgg gaa aga tta aga gag gaa ata gat gcc aat ttt gga ttg 1008Thr Ile Trp Glu Arg Leu Arg Glu Glu Ile Asp Ala Asn Phe Gly Leu 325 330 335gaa tca ccc gac ttg ctt aca ttt gat tca ctt cgc aag ttc aaa tat 1056Glu Ser Pro Asp Leu Leu Thr Phe Asp Ser Leu Arg Lys Phe Lys Tyr 340 345 350gtt caa gct atc ttg aat gag act ctt agg atg tac cct gga gtt cca 1104Val Gln Ala Ile Leu Asn Glu Thr Leu Arg Met Tyr Pro Gly Val Pro 355 360 365aga aat atg aaa act gct aaa tgt act act aca tta cca aaa gga gga 1152Arg Asn Met Lys Thr Ala Lys Cys Thr Thr Thr Leu Pro Lys Gly Gly 370 375 380ggg aaa gat ggt caa gat cca att cta gtt aaa aag ggt caa tct gtt 1200Gly Lys Asp Gly Gln Asp Pro Ile Leu Val Lys Lys Gly Gln Ser Val385 390 395 400ggg ttt att tcc ata gct act cat ttg gat ccg gtt tat ttt ggt agt 1248Gly Phe Ile Ser Ile Ala Thr His Leu Asp Pro Val Tyr Phe Gly Ser 405 410 415gat gcc cat gtg ttt aga cca gac cgc tgg ttt gat tct agt atg aaa 1296Asp Ala His Val Phe Arg Pro Asp Arg Trp Phe Asp Ser Ser Met Lys 420 425 430aat ttg ggg tgt aaa tac ttg ccc ttt aac gtc ggt cca aga acg tgt 1344Asn Leu Gly Cys Lys Tyr Leu Pro Phe Asn Val Gly Pro Arg Thr Cys 435 440 445ttg gga caa cag tac act ttg att gag gca agc tac ttg tta gtt cgt 1392Leu Gly Gln Gln Tyr Thr Leu Ile Glu Ala Ser Tyr Leu Leu Val Arg 450 455 460cta gcg caa aca tat gaa aca gtt gaa tca cat ccc gat tca gtt tat 1440Leu Ala Gln Thr Tyr Glu Thr Val Glu Ser His Pro Asp Ser Val Tyr465 470 475 480cca cca agg aag aaa gcg ttg atc aat atg tgt gct gca gac ggt gtt 1488Pro Pro Arg Lys Lys Ala Leu Ile Asn Met Cys Ala Ala Asp Gly Val 485 490 495gat gtc aag ttc cat aga tta taa 1512Asp Val Lys Phe His Arg Leu 50038503PRTCandida tropicalis 38Met Ser Leu Thr Glu Thr Thr Ala Thr Phe Ile Tyr Asn Tyr Trp Tyr1 5 10 15Ile Ile Phe Ile Ile Phe Tyr Thr Thr Ser Lys Ile Ile Lys Tyr His 20 25 30His Thr Thr Tyr Leu Met Ile Lys Phe Lys Ala Ser Pro Pro Leu Asn 35 40 45Tyr Ile Asn Lys Gly Phe Phe Gly Ile Gln Ala Thr Phe Thr Glu Leu 50 55 60Lys His Leu Ile Cys His Thr Ser Ile Asp Tyr Ala Ile Asp Gln Phe65 70 75 80Asn Asn Val Pro Phe Pro His Val His Thr Phe Val Thr Lys Val Leu 85 90 95Gly Asn Glu Leu Ile Met Thr Lys Asp Pro Glu Asn Ile Lys Val Leu 100 105 110Leu Ser Ser Ser Phe Asp Lys Phe Asp Tyr Gly Thr Arg Ser Ser Ala 115 120 125Val Gln Pro Ser Leu Gly Met Gly Ile Phe Thr Leu Glu Gly Glu Asn 130 135 140Trp Lys Ala Thr Arg Ser Val Leu Arg Asn Met Phe Asp Arg Lys Ser145 150 155 160Ile Asp Lys Val His Asp Phe Glu Pro His Phe Lys Thr Leu Gln Lys 165 170 175Arg Ile Asp Gly Lys Val Gly Tyr Phe Asp Ile Gln Gln Glu Phe Leu 180 185 190Lys Leu Gly Leu Glu Leu Ser Ile Glu Phe Ile Phe Gly Gln Val Val 195 200 205Ser Glu Asp Val Pro His Tyr Asp Asp Phe Thr Gln Ala Trp Asp Arg 210 215 220Cys Gln Asp Tyr Met Met Leu Arg Leu Leu Leu Gly Asp Phe Tyr Trp225 230 235 240Ile Ala Asn Asp Trp Arg Tyr Lys Gln Ser Asn Gln Ile Val Gln Ala 245 250 255Phe Cys Asp Tyr Leu Val Gln Lys Ser Leu Glu Asn Thr Cys Asn Asp 260 265 270Lys Phe Val Phe Val Gln Glu Leu Ala Lys His Thr Thr Asn Lys Thr 275 280 285Phe Ile Arg Asp Gln Ala Leu Ser Leu Ile Met Ala Ser Arg Asp Thr 290 295 300Thr Ala Glu Leu Met Ala Phe Thr Ile Leu Glu Leu Ser Arg Asn Pro305 310 315 320Thr Ile Trp Glu Arg Leu Arg Glu Glu Ile Asp Ala Asn Phe Gly Leu 325 330 335Glu Ser Pro Asp Leu Leu Thr Phe Asp Ser Leu Arg Lys Phe Lys Tyr 340 345 350Val Gln Ala Ile Leu Asn Glu Thr Leu Arg Met Tyr Pro Gly Val Pro 355 360 365Arg Asn Met Lys Thr Ala Lys Cys Thr Thr Thr Leu Pro Lys Gly Gly 370 375 380Gly Lys Asp Gly Gln Asp Pro Ile Leu Val Lys Lys Gly Gln Ser Val385 390 395 400Gly Phe Ile Ser Ile Ala Thr His Leu Asp Pro Val Tyr Phe Gly Ser 405 410 415Asp Ala His Val Phe Arg Pro Asp Arg Trp Phe Asp Ser Ser Met Lys 420 425 430Asn Leu Gly Cys Lys Tyr Leu Pro Phe Asn Val Gly Pro Arg Thr Cys 435 440 445Leu Gly Gln Gln Tyr Thr Leu Ile Glu Ala Ser Tyr Leu Leu Val Arg 450 455 460Leu Ala Gln Thr Tyr Glu Thr Val Glu Ser His Pro Asp Ser Val Tyr465 470 475 480Pro Pro Arg Lys Lys Ala Leu Ile Asn Met Cys Ala Ala Asp Gly Val 485 490 495Asp Val Lys Phe His Arg Leu 500391554DNACandida tropicalisCDS(1)..(1551) 39atg atc gaa caa gtt gtt gaa tac tgg tac gtg gtc tta cca ttg gta 48Met Ile Glu Gln Val Val Glu Tyr Trp Tyr Val Val Leu Pro Leu Val1 5 10 15ttt atc ctt cat aaa gta ttt gac atg tgg cac act cgt cgg ttg atg 96Phe Ile Leu His Lys Val Phe Asp Met Trp His Thr Arg Arg Leu Met 20 25 30aag caa ttg ggc gct gct cct gtc aca aac caa tta cac gac aat ttt 144Lys Gln Leu Gly Ala Ala Pro Val Thr Asn Gln Leu His Asp Asn Phe 35 40 45ttt ggt att atc aac gga tgg aaa gca ctt aag ttc aag aaa gaa ggt 192Phe Gly Ile Ile Asn Gly Trp Lys Ala Leu Lys Phe Lys Lys Glu Gly 50 55 60aga gct caa gaa tat aat gat tat aaa ttt gcc aat tcg aaa att cca 240Arg Ala Gln Glu Tyr Asn Asp Tyr Lys Phe Ala Asn Ser Lys Ile Pro65 70 75 80agt gtg ggt act tat gtt agt acc atc ttt gga aca aag ctc ctc gtc 288Ser Val Gly Thr Tyr Val Ser Thr Ile Phe Gly Thr Lys Leu Leu Val 85 90 95aca aaa gat ccg gag aat atc aaa gct tta tta gca acc caa ttc agt 336Thr Lys Asp Pro Glu Asn Ile Lys Ala Leu Leu Ala Thr Gln Phe Ser 100 105 110gat ttt tcc ttg ggt aag agg cat aca ctt ttc aaa cca tta tta ggt 384Asp Phe Ser Leu Gly Lys Arg His Thr Leu Phe Lys Pro Leu Leu Gly 115 120 125gac ggt att ttc act ttg gat gga gaa ggt tgg aaa cat agt cga gct 432Asp Gly Ile Phe Thr Leu Asp Gly Glu Gly Trp Lys His Ser Arg Ala 130 135 140atg ttg aga cca cag ttt gca aga gaa caa gtt gcc cat gtc act tct 480Met Leu Arg Pro Gln Phe Ala Arg Glu Gln Val Ala His Val Thr Ser145 150 155 160tta gag cca cat ttc caa ttg ttg aaa aaa cat atc ctc aag aac aaa 528Leu Glu Pro His Phe Gln Leu Leu Lys Lys His Ile Leu Lys Asn Lys 165 170 175gga ggt ttt ttt gat atc cag gaa ttg ttt ttc cga ttc act gtt gat 576Gly Gly Phe Phe Asp Ile Gln Glu Leu Phe Phe Arg Phe Thr Val Asp 180 185 190tca gct act gag ttt ttg ttt ggt gaa tca gta cac tct ttg aag gat 624Ser Ala Thr Glu Phe Leu Phe Gly Glu Ser Val His Ser Leu Lys Asp 195 200 205gaa aca att ggg tat aac caa gat gat atc gac ttt gtt ggt aga aag 672Glu Thr Ile Gly Tyr Asn Gln Asp Asp Ile Asp Phe Val Gly Arg Lys 210 215 220gat ttt gcg gaa tcg ttc aac aag gca caa gag tat ctt gct att aga 720Asp Phe Ala Glu Ser Phe Asn Lys Ala Gln Glu Tyr Leu Ala Ile Arg225 230 235 240act ttg gtg caa gat ttt tat tat ctt gta aac aat cag gaa ttc aga 768Thr Leu Val Gln Asp Phe Tyr Tyr Leu Val Asn Asn Gln Glu Phe Arg 245 250 255gat tgc aat aaa ctg gta cac aag ttt acc aac tac tat gtc caa aga 816Asp Cys Asn Lys Leu Val His Lys Phe Thr Asn Tyr Tyr Val Gln Arg 260 265 270gca ttg gat gcc act cct gaa gag ctt gaa aag caa agt gga tat gtt 864Ala Leu Asp Ala Thr Pro Glu Glu Leu Glu Lys Gln Ser Gly Tyr Val 275 280 285ttc ttg tat gaa ttg gtt aaa caa acc aga gac cct aat gta ttg aga 912Phe Leu Tyr Glu Leu Val Lys Gln Thr Arg Asp Pro Asn Val Leu Arg 290 295 300gat caa tca ttg aac atc tta tta gct ggt aga gat acc act gct ggg 960Asp Gln Ser Leu Asn Ile Leu Leu Ala Gly Arg Asp Thr Thr Ala Gly305 310 315 320ttg ttg tca ttt gcg gta ttt gaa ctt gct agg aat cca cat att tgg 1008Leu Leu Ser Phe Ala Val Phe Glu Leu Ala Arg Asn Pro His Ile Trp 325 330 335gcc aaa tta aga gaa gat gtt gaa tcc caa ttt ggt ctt ggt gaa gaa 1056Ala Lys Leu Arg Glu Asp Val Glu Ser Gln Phe Gly Leu Gly Glu Glu 340 345 350tct cgc att gaa gag att acc ttt gaa agt tta aaa cga tgt gaa tac 1104Ser Arg Ile Glu Glu Ile Thr Phe Glu Ser Leu Lys Arg Cys Glu Tyr 355 360 365ttg aaa gct ttc ctt aac gaa aca tta cgt gtt tat cca agt gtt cca 1152Leu Lys Ala Phe Leu Asn Glu Thr Leu Arg Val Tyr Pro Ser Val Pro 370 375 380aga aat ttc aga att gct acc aaa aac acc act tta cca aga ggt ggt 1200Arg Asn Phe Arg Ile Ala Thr Lys Asn Thr Thr Leu Pro Arg Gly Gly385 390 395 400ggt tca gac ggc aat tct cct gtt ttg gtc aaa aag ggc gag gct gtt 1248Gly Ser Asp Gly Asn Ser Pro Val Leu Val Lys Lys Gly Glu Ala Val 405 410 415tca tat ggt ata aat tct act cac tta gat cct gtc tat tat ggt gac 1296Ser Tyr Gly Ile Asn Ser Thr His Leu Asp Pro Val Tyr Tyr Gly Asp 420 425 430gat gct gca gaa ttt aga cca gaa aga tgg aac gag cca tca aca aga 1344Asp Ala Ala Glu Phe Arg Pro Glu Arg Trp Asn Glu Pro Ser Thr Arg 435 440 445aaa ttg gga tgg gca tat tta ccg ttc aac gga ggc cca aga att tgt 1392Lys Leu Gly Trp Ala Tyr Leu Pro Phe Asn Gly Gly Pro Arg Ile Cys 450 455 460tta ggt caa caa ttt gct tta acc gaa gcg ggt tat gta ttg gtt aga 1440Leu Gly Gln Gln Phe Ala Leu Thr Glu Ala Gly Tyr Val Leu Val Arg465 470 475 480ttg gcc caa agt ttt gac acc ttg gaa ttg aag cca cca gtt gtg tat 1488Leu Ala Gln Ser

Phe Asp Thr Leu Glu Leu Lys Pro Pro Val Val Tyr 485 490 495cca cca aag aga tta aca aac ttg act atg tct tta caa gac gga act 1536Pro Pro Lys Arg Leu Thr Asn Leu Thr Met Ser Leu Gln Asp Gly Thr 500 505 510att gtc aag atc gat tag 1554Ile Val Lys Ile Asp 51540517PRTCandida tropicalis 40Met Ile Glu Gln Val Val Glu Tyr Trp Tyr Val Val Leu Pro Leu Val1 5 10 15Phe Ile Leu His Lys Val Phe Asp Met Trp His Thr Arg Arg Leu Met 20 25 30Lys Gln Leu Gly Ala Ala Pro Val Thr Asn Gln Leu His Asp Asn Phe 35 40 45Phe Gly Ile Ile Asn Gly Trp Lys Ala Leu Lys Phe Lys Lys Glu Gly 50 55 60Arg Ala Gln Glu Tyr Asn Asp Tyr Lys Phe Ala Asn Ser Lys Ile Pro65 70 75 80Ser Val Gly Thr Tyr Val Ser Thr Ile Phe Gly Thr Lys Leu Leu Val 85 90 95Thr Lys Asp Pro Glu Asn Ile Lys Ala Leu Leu Ala Thr Gln Phe Ser 100 105 110Asp Phe Ser Leu Gly Lys Arg His Thr Leu Phe Lys Pro Leu Leu Gly 115 120 125Asp Gly Ile Phe Thr Leu Asp Gly Glu Gly Trp Lys His Ser Arg Ala 130 135 140Met Leu Arg Pro Gln Phe Ala Arg Glu Gln Val Ala His Val Thr Ser145 150 155 160Leu Glu Pro His Phe Gln Leu Leu Lys Lys His Ile Leu Lys Asn Lys 165 170 175Gly Gly Phe Phe Asp Ile Gln Glu Leu Phe Phe Arg Phe Thr Val Asp 180 185 190Ser Ala Thr Glu Phe Leu Phe Gly Glu Ser Val His Ser Leu Lys Asp 195 200 205Glu Thr Ile Gly Tyr Asn Gln Asp Asp Ile Asp Phe Val Gly Arg Lys 210 215 220Asp Phe Ala Glu Ser Phe Asn Lys Ala Gln Glu Tyr Leu Ala Ile Arg225 230 235 240Thr Leu Val Gln Asp Phe Tyr Tyr Leu Val Asn Asn Gln Glu Phe Arg 245 250 255Asp Cys Asn Lys Leu Val His Lys Phe Thr Asn Tyr Tyr Val Gln Arg 260 265 270Ala Leu Asp Ala Thr Pro Glu Glu Leu Glu Lys Gln Ser Gly Tyr Val 275 280 285Phe Leu Tyr Glu Leu Val Lys Gln Thr Arg Asp Pro Asn Val Leu Arg 290 295 300Asp Gln Ser Leu Asn Ile Leu Leu Ala Gly Arg Asp Thr Thr Ala Gly305 310 315 320Leu Leu Ser Phe Ala Val Phe Glu Leu Ala Arg Asn Pro His Ile Trp 325 330 335Ala Lys Leu Arg Glu Asp Val Glu Ser Gln Phe Gly Leu Gly Glu Glu 340 345 350Ser Arg Ile Glu Glu Ile Thr Phe Glu Ser Leu Lys Arg Cys Glu Tyr 355 360 365Leu Lys Ala Phe Leu Asn Glu Thr Leu Arg Val Tyr Pro Ser Val Pro 370 375 380Arg Asn Phe Arg Ile Ala Thr Lys Asn Thr Thr Leu Pro Arg Gly Gly385 390 395 400Gly Ser Asp Gly Asn Ser Pro Val Leu Val Lys Lys Gly Glu Ala Val 405 410 415Ser Tyr Gly Ile Asn Ser Thr His Leu Asp Pro Val Tyr Tyr Gly Asp 420 425 430Asp Ala Ala Glu Phe Arg Pro Glu Arg Trp Asn Glu Pro Ser Thr Arg 435 440 445Lys Leu Gly Trp Ala Tyr Leu Pro Phe Asn Gly Gly Pro Arg Ile Cys 450 455 460Leu Gly Gln Gln Phe Ala Leu Thr Glu Ala Gly Tyr Val Leu Val Arg465 470 475 480Leu Ala Gln Ser Phe Asp Thr Leu Glu Leu Lys Pro Pro Val Val Tyr 485 490 495Pro Pro Lys Arg Leu Thr Asn Leu Thr Met Ser Leu Gln Asp Gly Thr 500 505 510Ile Val Lys Ile Asp 515411539DNACandida tropicalisCDS(1)..(1536) 41atg att gaa cag gtt tta cat tat tgg tat att gtt tta cct gca ttt 48Met Ile Glu Gln Val Leu His Tyr Trp Tyr Ile Val Leu Pro Ala Phe1 5 10 15ata att ttt cat tgg att gta tct gca att cat aca aat tcc ttg cgt 96Ile Ile Phe His Trp Ile Val Ser Ala Ile His Thr Asn Ser Leu Arg 20 25 30aga aaa cta ggt gcc aaa cct ttc act cat aca caa ctt gat ggt ttt 144Arg Lys Leu Gly Ala Lys Pro Phe Thr His Thr Gln Leu Asp Gly Phe 35 40 45tat gga ttt aaa ttt ggc cgt gat ttt ctt aaa gct aaa agg att ggt 192Tyr Gly Phe Lys Phe Gly Arg Asp Phe Leu Lys Ala Lys Arg Ile Gly 50 55 60agg caa gtt gat tta atc aat tcc cgt ttc cca gac gat att gac aca 240Arg Gln Val Asp Leu Ile Asn Ser Arg Phe Pro Asp Asp Ile Asp Thr65 70 75 80ttt tca agt tat act ttc ggc aac cac gtg att ttt aca cgt gat cca 288Phe Ser Ser Tyr Thr Phe Gly Asn His Val Ile Phe Thr Arg Asp Pro 85 90 95gaa aat atc aaa gct tta ttg gca aca caa ttt aat gat ttt tca tta 336Glu Asn Ile Lys Ala Leu Leu Ala Thr Gln Phe Asn Asp Phe Ser Leu 100 105 110ggt ggt aga att aag ttt ttc aaa cca ttg ttg gga tat gga atc ttt 384Gly Gly Arg Ile Lys Phe Phe Lys Pro Leu Leu Gly Tyr Gly Ile Phe 115 120 125act ttg gat gga gaa ggt tgg aaa cat agt cga gct atg ttg aga cca 432Thr Leu Asp Gly Glu Gly Trp Lys His Ser Arg Ala Met Leu Arg Pro 130 135 140cag ttt gca aga gaa caa gtt gcc cat gtc act tct tta gaa cca cat 480Gln Phe Ala Arg Glu Gln Val Ala His Val Thr Ser Leu Glu Pro His145 150 155 160ttc caa ttg ttg aaa aag cat atc ctc aag aac aaa ggt ggg ttt ttt 528Phe Gln Leu Leu Lys Lys His Ile Leu Lys Asn Lys Gly Gly Phe Phe 165 170 175gat atc cag gaa ttg ttt ttc cga ttc acc gtt gat tca gct act gag 576Asp Ile Gln Glu Leu Phe Phe Arg Phe Thr Val Asp Ser Ala Thr Glu 180 185 190ttt ttg ttt ggt gaa tca gtg aac tct ttg aaa agt gca tca att ggt 624Phe Leu Phe Gly Glu Ser Val Asn Ser Leu Lys Ser Ala Ser Ile Gly 195 200 205tgt gac gag gaa acc gag ctt gag gaa aga aag aaa ttt gcg gaa gca 672Cys Asp Glu Glu Thr Glu Leu Glu Glu Arg Lys Lys Phe Ala Glu Ala 210 215 220ttc aat aaa gcg caa gag tat att tct act cga gtt gct ttg caa caa 720Phe Asn Lys Ala Gln Glu Tyr Ile Ser Thr Arg Val Ala Leu Gln Gln225 230 235 240tta tat tgg ttt gtt aat aat agc gaa ttc aag gaa tgt aac gaa att 768Leu Tyr Trp Phe Val Asn Asn Ser Glu Phe Lys Glu Cys Asn Glu Ile 245 250 255gtt cat aag ttt acc aat tat tat gta caa aag gca ttg gat gct act 816Val His Lys Phe Thr Asn Tyr Tyr Val Gln Lys Ala Leu Asp Ala Thr 260 265 270cct gaa gag ctt gaa aag caa agt gga tat gtt ttc ttg tat gaa ttg 864Pro Glu Glu Leu Glu Lys Gln Ser Gly Tyr Val Phe Leu Tyr Glu Leu 275 280 285gtt aaa caa acc aga gac cct aat gta ttg aga gat caa tca ttg aat 912Val Lys Gln Thr Arg Asp Pro Asn Val Leu Arg Asp Gln Ser Leu Asn 290 295 300atc tta tta gct ggt aga gat acc act gct ggg ttg ttg tca ttt gcg 960Ile Leu Leu Ala Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Ala305 310 315 320gta ttt gaa ctt gct agg aat cca cat att tgg gcc aaa tta aga gaa 1008Val Phe Glu Leu Ala Arg Asn Pro His Ile Trp Ala Lys Leu Arg Glu 325 330 335gat gtc gaa tcc caa ttt ggt ctt ggt gaa gaa tct cgc att gaa gag 1056Asp Val Glu Ser Gln Phe Gly Leu Gly Glu Glu Ser Arg Ile Glu Glu 340 345 350att acc ttt gaa agt tta aaa cga tgt gaa tat ttg aaa gcc gtg atg 1104Ile Thr Phe Glu Ser Leu Lys Arg Cys Glu Tyr Leu Lys Ala Val Met 355 360 365aat gaa aca ttg aga ttg cat cca agt gtt cca aga aat gct aga ttt 1152Asn Glu Thr Leu Arg Leu His Pro Ser Val Pro Arg Asn Ala Arg Phe 370 375 380gca ctt aag gat aca act tta cct aga ggt gga ggt cca gat gga aaa 1200Ala Leu Lys Asp Thr Thr Leu Pro Arg Gly Gly Gly Pro Asp Gly Lys385 390 395 400gac ccg att tta gtt aga aaa aat gaa gtt gtt caa tat tcc att tct 1248Asp Pro Ile Leu Val Arg Lys Asn Glu Val Val Gln Tyr Ser Ile Ser 405 410 415ggc aca caa att gat cca aaa cat tat ggc aaa gat gct aaa ttg ttt 1296Gly Thr Gln Ile Asp Pro Lys His Tyr Gly Lys Asp Ala Lys Leu Phe 420 425 430aga cca gaa aga tgg ttt gaa tca agt aca aga aat tta ggt tgg gca 1344Arg Pro Glu Arg Trp Phe Glu Ser Ser Thr Arg Asn Leu Gly Trp Ala 435 440 445tac tta cca ttc aac ggg ggt ccg aga att tgt tta ggt caa caa ttt 1392Tyr Leu Pro Phe Asn Gly Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe 450 455 460gct tta acc gaa gca ggt tac ata ttg gtt aga ttg gct caa agt ttt 1440Ala Leu Thr Glu Ala Gly Tyr Ile Leu Val Arg Leu Ala Gln Ser Phe465 470 475 480gac acc ttg gaa ttg aaa cca gat aca gaa tac cct cca cca aga tta 1488Asp Thr Leu Glu Leu Lys Pro Asp Thr Glu Tyr Pro Pro Pro Arg Leu 485 490 495gcc cat ttg act atg tgt ttg ttt gat ggt gcg ctt gtc aag atg gat 1536Ala His Leu Thr Met Cys Leu Phe Asp Gly Ala Leu Val Lys Met Asp 500 505 510taa 1539 42512PRTCandida tropicalis 42Met Ile Glu Gln Val Leu His Tyr Trp Tyr Ile Val Leu Pro Ala Phe1 5 10 15Ile Ile Phe His Trp Ile Val Ser Ala Ile His Thr Asn Ser Leu Arg 20 25 30Arg Lys Leu Gly Ala Lys Pro Phe Thr His Thr Gln Leu Asp Gly Phe 35 40 45Tyr Gly Phe Lys Phe Gly Arg Asp Phe Leu Lys Ala Lys Arg Ile Gly 50 55 60Arg Gln Val Asp Leu Ile Asn Ser Arg Phe Pro Asp Asp Ile Asp Thr65 70 75 80Phe Ser Ser Tyr Thr Phe Gly Asn His Val Ile Phe Thr Arg Asp Pro 85 90 95Glu Asn Ile Lys Ala Leu Leu Ala Thr Gln Phe Asn Asp Phe Ser Leu 100 105 110Gly Gly Arg Ile Lys Phe Phe Lys Pro Leu Leu Gly Tyr Gly Ile Phe 115 120 125Thr Leu Asp Gly Glu Gly Trp Lys His Ser Arg Ala Met Leu Arg Pro 130 135 140Gln Phe Ala Arg Glu Gln Val Ala His Val Thr Ser Leu Glu Pro His145 150 155 160Phe Gln Leu Leu Lys Lys His Ile Leu Lys Asn Lys Gly Gly Phe Phe 165 170 175Asp Ile Gln Glu Leu Phe Phe Arg Phe Thr Val Asp Ser Ala Thr Glu 180 185 190Phe Leu Phe Gly Glu Ser Val Asn Ser Leu Lys Ser Ala Ser Ile Gly 195 200 205Cys Asp Glu Glu Thr Glu Leu Glu Glu Arg Lys Lys Phe Ala Glu Ala 210 215 220Phe Asn Lys Ala Gln Glu Tyr Ile Ser Thr Arg Val Ala Leu Gln Gln225 230 235 240Leu Tyr Trp Phe Val Asn Asn Ser Glu Phe Lys Glu Cys Asn Glu Ile 245 250 255Val His Lys Phe Thr Asn Tyr Tyr Val Gln Lys Ala Leu Asp Ala Thr 260 265 270Pro Glu Glu Leu Glu Lys Gln Ser Gly Tyr Val Phe Leu Tyr Glu Leu 275 280 285Val Lys Gln Thr Arg Asp Pro Asn Val Leu Arg Asp Gln Ser Leu Asn 290 295 300Ile Leu Leu Ala Gly Arg Asp Thr Thr Ala Gly Leu Leu Ser Phe Ala305 310 315 320Val Phe Glu Leu Ala Arg Asn Pro His Ile Trp Ala Lys Leu Arg Glu 325 330 335Asp Val Glu Ser Gln Phe Gly Leu Gly Glu Glu Ser Arg Ile Glu Glu 340 345 350Ile Thr Phe Glu Ser Leu Lys Arg Cys Glu Tyr Leu Lys Ala Val Met 355 360 365Asn Glu Thr Leu Arg Leu His Pro Ser Val Pro Arg Asn Ala Arg Phe 370 375 380Ala Leu Lys Asp Thr Thr Leu Pro Arg Gly Gly Gly Pro Asp Gly Lys385 390 395 400Asp Pro Ile Leu Val Arg Lys Asn Glu Val Val Gln Tyr Ser Ile Ser 405 410 415Gly Thr Gln Ile Asp Pro Lys His Tyr Gly Lys Asp Ala Lys Leu Phe 420 425 430Arg Pro Glu Arg Trp Phe Glu Ser Ser Thr Arg Asn Leu Gly Trp Ala 435 440 445Tyr Leu Pro Phe Asn Gly Gly Pro Arg Ile Cys Leu Gly Gln Gln Phe 450 455 460Ala Leu Thr Glu Ala Gly Tyr Ile Leu Val Arg Leu Ala Gln Ser Phe465 470 475 480Asp Thr Leu Glu Leu Lys Pro Asp Thr Glu Tyr Pro Pro Pro Arg Leu 485 490 495Ala His Leu Thr Met Cys Leu Phe Asp Gly Ala Leu Val Lys Met Asp 500 505 510431572DNACandida tropicalisCDS(1)..(1569) 43atg att aaa gaa ata gtg tat ttt gta tat tat tat gga att acc cac 48Met Ile Lys Glu Ile Val Tyr Phe Val Tyr Tyr Tyr Gly Ile Thr His1 5 10 15tca gtt tca gtg cag gtc acc gct ctt gtc ctt atc gtg aca tac ttt 96Ser Val Ser Val Gln Val Thr Ala Leu Val Leu Ile Val Thr Tyr Phe 20 25 30ttt gtt att cgt cca att aac tca cct tta tgg aga gtt ccc gga cca 144Phe Val Ile Arg Pro Ile Asn Ser Pro Leu Trp Arg Val Pro Gly Pro 35 40 45tat tta cat cgg gta act tac ttt cca tgt tta aat gcc caa cga aag 192Tyr Leu His Arg Val Thr Tyr Phe Pro Cys Leu Asn Ala Gln Arg Lys 50 55 60gga gaa tgg atc tcc aaa gtt tat gat ttg cac aag aaa tat ggt gat 240Gly Glu Trp Ile Ser Lys Val Tyr Asp Leu His Lys Lys Tyr Gly Asp65 70 75 80gta gtt tta ctt tct cca aat gaa atc agc gtc aat ggt gac cca aaa 288Val Val Leu Leu Ser Pro Asn Glu Ile Ser Val Asn Gly Asp Pro Lys 85 90 95tat ttg act gat att tat gta aag aac ctc cca aag tca aag ttt tat 336Tyr Leu Thr Asp Ile Tyr Val Lys Asn Leu Pro Lys Ser Lys Phe Tyr 100 105 110gaa aac ttt aga aat cat gga ttc cag gat aat att ttt gcc agt ttg 384Glu Asn Phe Arg Asn His Gly Phe Gln Asp Asn Ile Phe Ala Ser Leu 115 120 125gaa aat gat aga cat atc aag tat aaa aga atg ata aat aac ttg tac 432Glu Asn Asp Arg His Ile Lys Tyr Lys Arg Met Ile Asn Asn Leu Tyr 130 135 140agt aaa tct tcc atc ttc tcc aaa gaa aac cac aca aga tca gtt tta 480Ser Lys Ser Ser Ile Phe Ser Lys Glu Asn His Thr Arg Ser Val Leu145 150 155 160ttt gac acc aca aaa aca tta gtc gat gca gtt gct aga gaa tct ccg 528Phe Asp Thr Thr Lys Thr Leu Val Asp Ala Val Ala Arg Glu Ser Pro 165 170 175tca att gat gtg ttt acg tta ttt ggt tcc ttg gcc atg gat gtg gtt 576Ser Ile Asp Val Phe Thr Leu Phe Gly Ser Leu Ala Met Asp Val Val 180 185 190tca agg ttt gaa ctt ggt aga gac aat gga acg gat tta ttg aat cat 624Ser Arg Phe Glu Leu Gly Arg Asp Asn Gly Thr Asp Leu Leu Asn His 195 200 205cca caa gaa agg cat att att gaa tct cat aga aag gtg tct tgt atg 672Pro Gln Glu Arg His Ile Ile Glu Ser His Arg Lys Val Ser Cys Met 210 215 220ggg ttc tgg aca aca ttg atg cca agc ttc ttt tgg aat ttg gca gca 720Gly Phe Trp Thr Thr Leu Met Pro Ser Phe Phe Trp Asn Leu Ala Ala225 230 235 240acc aag gcc acc ttg caa gct gtt gat gat att tgc aat ttc caa ttg 768Thr Lys Ala Thr Leu Gln Ala Val Asp Asp Ile Cys Asn Phe Gln Leu 245 250 255ggt tta tat aaa att gca gaa tcc aat ctt gtg tcc aat ggt aaa aac 816Gly Leu Tyr Lys Ile Ala Glu Ser Asn Leu Val Ser Asn Gly Lys Asn 260 265 270cta act acg ata caa aca ctc aag aag tac gga ttg gaa gga aat tct 864Leu Thr Thr Ile Gln Thr Leu Lys Lys Tyr Gly Leu Glu Gly Asn Ser 275 280 285gcg tat tct ttt ctc acg gat aat tta ttt gct gga cat gaa act aca 912Ala Tyr Ser Phe Leu Thr Asp Asn Leu Phe Ala Gly His Glu Thr Thr 290 295 300gct gtt caa ttg aca tat ttg tgt tat gaa tta tca aga cca gct aat 960Ala Val Gln Leu Thr Tyr Leu Cys Tyr Glu Leu Ser Arg Pro Ala Asn305 310 315 320tac aaa att cag aat aga tta aga tat gag ctt caa gaa gca ttt cca 1008Tyr Lys Ile Gln Asn Arg Leu Arg Tyr Glu Leu Gln Glu Ala Phe Pro 325 330 335agt ggt caa att gaa

gat ttg gaa gtg gtt gat aat ctt ccg tat ctt 1056Ser Gly Gln Ile Glu Asp Leu Glu Val Val Asp Asn Leu Pro Tyr Leu 340 345 350aat gcg ttg ttg tcc gaa aat ggt cga att cac acc tca att cct gga 1104Asn Ala Leu Leu Ser Glu Asn Gly Arg Ile His Thr Ser Ile Pro Gly 355 360 365gct gag cca cgt gtg gtt gca aaa ccg tat act att ggc aaa ttg ctt 1152Ala Glu Pro Arg Val Val Ala Lys Pro Tyr Thr Ile Gly Lys Leu Leu 370 375 380att cca gtt ggt acg gtt atc tct tgt ctt cct tat gcg tat cat aga 1200Ile Pro Val Gly Thr Val Ile Ser Cys Leu Pro Tyr Ala Tyr His Arg385 390 395 400aat ccg tcg gta ttt acc aat cct gat aaa ttt atc ccc gaa aga tgg 1248Asn Pro Ser Val Phe Thr Asn Pro Asp Lys Phe Ile Pro Glu Arg Trp 405 410 415tta gtt gac aac gaa gaa gac aag aaa cga gtc aaa caa caa gcg aag 1296Leu Val Asp Asn Glu Glu Asp Lys Lys Arg Val Lys Gln Gln Ala Lys 420 425 430tat atg atg cca ttt ggt aaa ggt gta aga atg tgt ctt ggg atg aac 1344Tyr Met Met Pro Phe Gly Lys Gly Val Arg Met Cys Leu Gly Met Asn 435 440 445ttg gca ctc att gaa atg aag ttg gcc att gca agt ttg tat tta aac 1392Leu Ala Leu Ile Glu Met Lys Leu Ala Ile Ala Ser Leu Tyr Leu Asn 450 455 460ttt tct tct tcc att gac gaa gac tgg tgt ggc aaa gta tta gaa aat 1440Phe Ser Ser Ser Ile Asp Glu Asp Trp Cys Gly Lys Val Leu Glu Asn465 470 475 480gat gac ccc ata ggt atc ggg aat tca tgc act cat gag act gat cag 1488Asp Asp Pro Ile Gly Ile Gly Asn Ser Cys Thr His Glu Thr Asp Gln 485 490 495gat aaa atg aaa atg tac gat gct tat act acg aga cca atg cta gat 1536Asp Lys Met Lys Met Tyr Asp Ala Tyr Thr Thr Arg Pro Met Leu Asp 500 505 510gaa tgt tac ttg aag tgg aca aga tta act cct tga 1572Glu Cys Tyr Leu Lys Trp Thr Arg Leu Thr Pro 515 52044523PRTCandida tropicalis 44Met Ile Lys Glu Ile Val Tyr Phe Val Tyr Tyr Tyr Gly Ile Thr His1 5 10 15Ser Val Ser Val Gln Val Thr Ala Leu Val Leu Ile Val Thr Tyr Phe 20 25 30Phe Val Ile Arg Pro Ile Asn Ser Pro Leu Trp Arg Val Pro Gly Pro 35 40 45Tyr Leu His Arg Val Thr Tyr Phe Pro Cys Leu Asn Ala Gln Arg Lys 50 55 60Gly Glu Trp Ile Ser Lys Val Tyr Asp Leu His Lys Lys Tyr Gly Asp65 70 75 80Val Val Leu Leu Ser Pro Asn Glu Ile Ser Val Asn Gly Asp Pro Lys 85 90 95Tyr Leu Thr Asp Ile Tyr Val Lys Asn Leu Pro Lys Ser Lys Phe Tyr 100 105 110Glu Asn Phe Arg Asn His Gly Phe Gln Asp Asn Ile Phe Ala Ser Leu 115 120 125Glu Asn Asp Arg His Ile Lys Tyr Lys Arg Met Ile Asn Asn Leu Tyr 130 135 140Ser Lys Ser Ser Ile Phe Ser Lys Glu Asn His Thr Arg Ser Val Leu145 150 155 160Phe Asp Thr Thr Lys Thr Leu Val Asp Ala Val Ala Arg Glu Ser Pro 165 170 175Ser Ile Asp Val Phe Thr Leu Phe Gly Ser Leu Ala Met Asp Val Val 180 185 190Ser Arg Phe Glu Leu Gly Arg Asp Asn Gly Thr Asp Leu Leu Asn His 195 200 205Pro Gln Glu Arg His Ile Ile Glu Ser His Arg Lys Val Ser Cys Met 210 215 220Gly Phe Trp Thr Thr Leu Met Pro Ser Phe Phe Trp Asn Leu Ala Ala225 230 235 240Thr Lys Ala Thr Leu Gln Ala Val Asp Asp Ile Cys Asn Phe Gln Leu 245 250 255Gly Leu Tyr Lys Ile Ala Glu Ser Asn Leu Val Ser Asn Gly Lys Asn 260 265 270Leu Thr Thr Ile Gln Thr Leu Lys Lys Tyr Gly Leu Glu Gly Asn Ser 275 280 285Ala Tyr Ser Phe Leu Thr Asp Asn Leu Phe Ala Gly His Glu Thr Thr 290 295 300Ala Val Gln Leu Thr Tyr Leu Cys Tyr Glu Leu Ser Arg Pro Ala Asn305 310 315 320Tyr Lys Ile Gln Asn Arg Leu Arg Tyr Glu Leu Gln Glu Ala Phe Pro 325 330 335Ser Gly Gln Ile Glu Asp Leu Glu Val Val Asp Asn Leu Pro Tyr Leu 340 345 350Asn Ala Leu Leu Ser Glu Asn Gly Arg Ile His Thr Ser Ile Pro Gly 355 360 365Ala Glu Pro Arg Val Val Ala Lys Pro Tyr Thr Ile Gly Lys Leu Leu 370 375 380Ile Pro Val Gly Thr Val Ile Ser Cys Leu Pro Tyr Ala Tyr His Arg385 390 395 400Asn Pro Ser Val Phe Thr Asn Pro Asp Lys Phe Ile Pro Glu Arg Trp 405 410 415Leu Val Asp Asn Glu Glu Asp Lys Lys Arg Val Lys Gln Gln Ala Lys 420 425 430Tyr Met Met Pro Phe Gly Lys Gly Val Arg Met Cys Leu Gly Met Asn 435 440 445Leu Ala Leu Ile Glu Met Lys Leu Ala Ile Ala Ser Leu Tyr Leu Asn 450 455 460Phe Ser Ser Ser Ile Asp Glu Asp Trp Cys Gly Lys Val Leu Glu Asn465 470 475 480Asp Asp Pro Ile Gly Ile Gly Asn Ser Cys Thr His Glu Thr Asp Gln 485 490 495Asp Lys Met Lys Met Tyr Asp Ala Tyr Thr Thr Arg Pro Met Leu Asp 500 505 510Glu Cys Tyr Leu Lys Trp Thr Arg Leu Thr Pro 515 520451728DNACandida tropicalisCDS(1)..(1725) 45atg ctt ctg agc ata cca tgg gat caa tca ttg cta acg ttg cta aca 48Met Leu Leu Ser Ile Pro Trp Asp Gln Ser Leu Leu Thr Leu Leu Thr1 5 10 15tat ctt gat acc cat cca ata gct acg ata ttc acc att att tta acc 96Tyr Leu Asp Thr His Pro Ile Ala Thr Ile Phe Thr Ile Ile Leu Thr 20 25 30att tta aca att gga att tta ttt gat tat tgt cta tca cct aaa gag 144Ile Leu Thr Ile Gly Ile Leu Phe Asp Tyr Cys Leu Ser Pro Lys Glu 35 40 45att gcc aat att ttc agt att cct gga gat ttg cca ttt att ggt cat 192Ile Ala Asn Ile Phe Ser Ile Pro Gly Asp Leu Pro Phe Ile Gly His 50 55 60tta cat tta ata cta gat aat cca gca ttg att tat tta aca tgg tat 240Leu His Leu Ile Leu Asp Asn Pro Ala Leu Ile Tyr Leu Thr Trp Tyr65 70 75 80aaa tta tat aat aaa ctg gtt ttt caa att cgt att gga aat aaa cgt 288Lys Leu Tyr Asn Lys Leu Val Phe Gln Ile Arg Ile Gly Asn Lys Arg 85 90 95gta gtt gtt gtt aat tca ttt gat gat gtt gtt gga tta tgg ata aat 336Val Val Val Val Asn Ser Phe Asp Asp Val Val Gly Leu Trp Ile Asn 100 105 110cat agt tgt caa aat aat tca aga cct tta agt tat act ttt cat gga 384His Ser Cys Gln Asn Asn Ser Arg Pro Leu Ser Tyr Thr Phe His Gly 115 120 125tta gtt tca gca tta caa ggt ttt aca gtt ggt tct act cct gca agt 432Leu Val Ser Ala Leu Gln Gly Phe Thr Val Gly Ser Thr Pro Ala Ser 130 135 140tta aca ttt ctg aga aag aag aaa gtt att tca ctg tgt tta aga aag 480Leu Thr Phe Leu Arg Lys Lys Lys Val Ile Ser Leu Cys Leu Arg Lys145 150 155 160aaa gaa att gat gaa aaa gta tgt ctc ata gat aat gaa att tgt gtt 528Lys Glu Ile Asp Glu Lys Val Cys Leu Ile Asp Asn Glu Ile Cys Val 165 170 175atg att aaa gaa ata att aaa aag aag gat ata tca act gat gta aat 576Met Ile Lys Glu Ile Ile Lys Lys Lys Asp Ile Ser Thr Asp Val Asn 180 185 190atg tta cct tat tta caa aaa ttc ata tta aaa aca gct att ctt atg 624Met Leu Pro Tyr Leu Gln Lys Phe Ile Leu Lys Thr Ala Ile Leu Met 195 200 205agt tat gga att gaa ttg gat tgt tat aat aaa gat gtt aaa tta tgt 672Ser Tyr Gly Ile Glu Leu Asp Cys Tyr Asn Lys Asp Val Lys Leu Cys 210 215 220caa gag att atc acc gtg gag aat aat atc ata aga tta aga tca cca 720Gln Glu Ile Ile Thr Val Glu Asn Asn Ile Ile Arg Leu Arg Ser Pro225 230 235 240ata tct aat ctt caa gat tct gta cct ttc tta aga cta ata cca tgg 768Ile Ser Asn Leu Gln Asp Ser Val Pro Phe Leu Arg Leu Ile Pro Trp 245 250 255ttt aat aat cga gaa ttt gcc ctt cgt tgt gga aat aga agg aat aaa 816Phe Asn Asn Arg Glu Phe Ala Leu Arg Cys Gly Asn Arg Arg Asn Lys 260 265 270tat atg gat caa tta tat aat agg tta caa aat gga tta gct gaa aat 864Tyr Met Asp Gln Leu Tyr Asn Arg Leu Gln Asn Gly Leu Ala Glu Asn 275 280 285gat cca aat ata gct aat agt att ctt gga caa tta att ctt aat aat 912Asp Pro Asn Ile Ala Asn Ser Ile Leu Gly Gln Leu Ile Leu Asn Asn 290 295 300gat aat aat aat tct aac agc tta act agt caa gaa ata caa agt att 960Asp Asn Asn Asn Ser Asn Ser Leu Thr Ser Gln Glu Ile Gln Ser Ile305 310 315 320tgt tta aca tta gta agt gct gga tta gat aat acc cca ctt aat ttg 1008Cys Leu Thr Leu Val Ser Ala Gly Leu Asp Asn Thr Pro Leu Asn Leu 325 330 335aat tat ctt att gga ata tta tca caa cca aga ata ggt aag ata ttt 1056Asn Tyr Leu Ile Gly Ile Leu Ser Gln Pro Arg Ile Gly Lys Ile Phe 340 345 350caa gat aaa gct ata aaa gat att tta aat cat gca aat gga gat att 1104Gln Asp Lys Ala Ile Lys Asp Ile Leu Asn His Ala Asn Gly Asp Ile 355 360 365att caa gca tgg aat caa ctg aat gaa gaa aat cga gat tgt aaa tat 1152Ile Gln Ala Trp Asn Gln Leu Asn Glu Glu Asn Arg Asp Cys Lys Tyr 370 375 380att caa gct tta att ctt gaa act tta aga cat ttt aca gta tta cca 1200Ile Gln Ala Leu Ile Leu Glu Thr Leu Arg His Phe Thr Val Leu Pro385 390 395 400tta agt tta cct aga tta act aca aaa cca ata tat tat aaa aat ttt 1248Leu Ser Leu Pro Arg Leu Thr Thr Lys Pro Ile Tyr Tyr Lys Asn Phe 405 410 415atg att cct aaa aat act cat atg ttt atg aat gca tat tct gca aat 1296Met Ile Pro Lys Asn Thr His Met Phe Met Asn Ala Tyr Ser Ala Asn 420 425 430cat gat gaa tta ata ttc aaa aat cca ttt aaa ttt gat cca gaa aga 1344His Asp Glu Leu Ile Phe Lys Asn Pro Phe Lys Phe Asp Pro Glu Arg 435 440 445tgg tta gat tca gaa act aat gaa att aaa tca aaa ata ctt gct act 1392Trp Leu Asp Ser Glu Thr Asn Glu Ile Lys Ser Lys Ile Leu Ala Thr 450 455 460act tct tcg tct tct tct tcg aca cat cat ggt ggt gga aat gga ata 1440Thr Ser Ser Ser Ser Ser Ser Thr His His Gly Gly Gly Asn Gly Ile465 470 475 480aat gta cag aat ttt cat ttt gca ttt ggt gct gga tca aga atg tgt 1488Asn Val Gln Asn Phe His Phe Ala Phe Gly Ala Gly Ser Arg Met Cys 485 490 495tca ggt tat aat cta gtt atg aaa gaa atg tat atg atg ata att aaa 1536Ser Gly Tyr Asn Leu Val Met Lys Glu Met Tyr Met Met Ile Ile Lys 500 505 510tta tta tta tta ttt gaa att aat cct cct gat aat aat aat aat aat 1584Leu Leu Leu Leu Phe Glu Ile Asn Pro Pro Asp Asn Asn Asn Asn Asn 515 520 525ggg aaa tat tta atg gaa atg aat cct ttt gtt aat aat ctg aat cct 1632Gly Lys Tyr Leu Met Glu Met Asn Pro Phe Val Asn Asn Leu Asn Pro 530 535 540aga ggt act tca ttt gaa cca cga ata cat aat att aaa tta caa tat 1680Arg Gly Thr Ser Phe Glu Pro Arg Ile His Asn Ile Lys Leu Gln Tyr545 550 555 560aga aaa cta cct aat tat gaa act cta cac gaa ata gtt ctc aaa tag 1728Arg Lys Leu Pro Asn Tyr Glu Thr Leu His Glu Ile Val Leu Lys 565 570 57546575PRTCandida tropicalis 46Met Leu Leu Ser Ile Pro Trp Asp Gln Ser Leu Leu Thr Leu Leu Thr1 5 10 15Tyr Leu Asp Thr His Pro Ile Ala Thr Ile Phe Thr Ile Ile Leu Thr 20 25 30Ile Leu Thr Ile Gly Ile Leu Phe Asp Tyr Cys Leu Ser Pro Lys Glu 35 40 45Ile Ala Asn Ile Phe Ser Ile Pro Gly Asp Leu Pro Phe Ile Gly His 50 55 60Leu His Leu Ile Leu Asp Asn Pro Ala Leu Ile Tyr Leu Thr Trp Tyr65 70 75 80Lys Leu Tyr Asn Lys Leu Val Phe Gln Ile Arg Ile Gly Asn Lys Arg 85 90 95Val Val Val Val Asn Ser Phe Asp Asp Val Val Gly Leu Trp Ile Asn 100 105 110His Ser Cys Gln Asn Asn Ser Arg Pro Leu Ser Tyr Thr Phe His Gly 115 120 125Leu Val Ser Ala Leu Gln Gly Phe Thr Val Gly Ser Thr Pro Ala Ser 130 135 140Leu Thr Phe Leu Arg Lys Lys Lys Val Ile Ser Leu Cys Leu Arg Lys145 150 155 160Lys Glu Ile Asp Glu Lys Val Cys Leu Ile Asp Asn Glu Ile Cys Val 165 170 175Met Ile Lys Glu Ile Ile Lys Lys Lys Asp Ile Ser Thr Asp Val Asn 180 185 190Met Leu Pro Tyr Leu Gln Lys Phe Ile Leu Lys Thr Ala Ile Leu Met 195 200 205Ser Tyr Gly Ile Glu Leu Asp Cys Tyr Asn Lys Asp Val Lys Leu Cys 210 215 220Gln Glu Ile Ile Thr Val Glu Asn Asn Ile Ile Arg Leu Arg Ser Pro225 230 235 240Ile Ser Asn Leu Gln Asp Ser Val Pro Phe Leu Arg Leu Ile Pro Trp 245 250 255Phe Asn Asn Arg Glu Phe Ala Leu Arg Cys Gly Asn Arg Arg Asn Lys 260 265 270Tyr Met Asp Gln Leu Tyr Asn Arg Leu Gln Asn Gly Leu Ala Glu Asn 275 280 285Asp Pro Asn Ile Ala Asn Ser Ile Leu Gly Gln Leu Ile Leu Asn Asn 290 295 300Asp Asn Asn Asn Ser Asn Ser Leu Thr Ser Gln Glu Ile Gln Ser Ile305 310 315 320Cys Leu Thr Leu Val Ser Ala Gly Leu Asp Asn Thr Pro Leu Asn Leu 325 330 335Asn Tyr Leu Ile Gly Ile Leu Ser Gln Pro Arg Ile Gly Lys Ile Phe 340 345 350Gln Asp Lys Ala Ile Lys Asp Ile Leu Asn His Ala Asn Gly Asp Ile 355 360 365Ile Gln Ala Trp Asn Gln Leu Asn Glu Glu Asn Arg Asp Cys Lys Tyr 370 375 380Ile Gln Ala Leu Ile Leu Glu Thr Leu Arg His Phe Thr Val Leu Pro385 390 395 400Leu Ser Leu Pro Arg Leu Thr Thr Lys Pro Ile Tyr Tyr Lys Asn Phe 405 410 415Met Ile Pro Lys Asn Thr His Met Phe Met Asn Ala Tyr Ser Ala Asn 420 425 430His Asp Glu Leu Ile Phe Lys Asn Pro Phe Lys Phe Asp Pro Glu Arg 435 440 445Trp Leu Asp Ser Glu Thr Asn Glu Ile Lys Ser Lys Ile Leu Ala Thr 450 455 460Thr Ser Ser Ser Ser Ser Ser Thr His His Gly Gly Gly Asn Gly Ile465 470 475 480Asn Val Gln Asn Phe His Phe Ala Phe Gly Ala Gly Ser Arg Met Cys 485 490 495Ser Gly Tyr Asn Leu Val Met Lys Glu Met Tyr Met Met Ile Ile Lys 500 505 510Leu Leu Leu Leu Phe Glu Ile Asn Pro Pro Asp Asn Asn Asn Asn Asn 515 520 525Gly Lys Tyr Leu Met Glu Met Asn Pro Phe Val Asn Asn Leu Asn Pro 530 535 540Arg Gly Thr Ser Phe Glu Pro Arg Ile His Asn Ile Lys Leu Gln Tyr545 550 555 560Arg Lys Leu Pro Asn Tyr Glu Thr Leu His Glu Ile Val Leu Lys 565 570 575471518DNACandida tropicalisCDS(1)..(1515) 47atg tat caa tta ttt tgt ttt ctt gct ggt ata att gtt gta tat aaa 48Met Tyr Gln Leu Phe Cys Phe Leu Ala Gly Ile Ile Val Val Tyr Lys1 5 10 15gca gca caa tac tac aag aga agg aca ctt gtg act aaa ttt cat tgc 96Ala Ala Gln Tyr Tyr Lys Arg Arg Thr Leu Val Thr Lys Phe His Cys 20 25 30aaa caa gca cgt att tcc cca aac aag tca tgg ttg gaa tat tta ggc 144Lys Gln Ala Arg Ile Ser Pro Asn Lys Ser Trp Leu Glu Tyr Leu Gly 35 40 45att gcc tcc gtt gta cat gcc aat gaa atg att aga aaa gga gga ttg 192Ile Ala Ser Val Val His Ala Asn Glu Met Ile Arg Lys Gly Gly Leu 50 55 60tat tca gaa att gat gga aga ttt aaa tcc ctt gat gtt tca aca ttc 240Tyr Ser Glu Ile Asp Gly Arg Phe Lys Ser Leu Asp Val Ser Thr Phe65 70 75 80aag tct ata act ttg gga aag aca acg tat gtt

acc aaa gat att gaa 288Lys Ser Ile Thr Leu Gly Lys Thr Thr Tyr Val Thr Lys Asp Ile Glu 85 90 95aac atc cgt cat atc ttg agt gca acg gaa atg aac tca tgg aat ctt 336Asn Ile Arg His Ile Leu Ser Ala Thr Glu Met Asn Ser Trp Asn Leu 100 105 110ggt gcc cgt cca att gcg tta aga ccc ttg att ggt gat gga att ttt 384Gly Ala Arg Pro Ile Ala Leu Arg Pro Leu Ile Gly Asp Gly Ile Phe 115 120 125gct agt gaa ggt caa tct tgg aaa cat agt cga atc atg ctt aga cca 432Ala Ser Glu Gly Gln Ser Trp Lys His Ser Arg Ile Met Leu Arg Pro 130 135 140gta ttt gca aaa gaa cac gtt aaa caa atc act tca atg gaa cca tat 480Val Phe Ala Lys Glu His Val Lys Gln Ile Thr Ser Met Glu Pro Tyr145 150 155 160gta caa ctg ttg atc aaa atc atc aag aac cat gaa ggg gaa cca tta 528Val Gln Leu Leu Ile Lys Ile Ile Lys Asn His Glu Gly Glu Pro Leu 165 170 175gag ttt caa acc tta gcc cat ctt ttt aca ata gat tat tct act gat 576Glu Phe Gln Thr Leu Ala His Leu Phe Thr Ile Asp Tyr Ser Thr Asp 180 185 190ttc cta tta ggt gaa agt tgt gat agt ttg aag gat ttc cta gga gaa 624Phe Leu Leu Gly Glu Ser Cys Asp Ser Leu Lys Asp Phe Leu Gly Glu 195 200 205gag tcc aat tcc aca tta gat aca tcg ttg aga ctg gca ttt gca tca 672Glu Ser Asn Ser Thr Leu Asp Thr Ser Leu Arg Leu Ala Phe Ala Ser 210 215 220cag ttt aat aaa acc cag cag caa atg aca att cga ttc atg ttg gga 720Gln Phe Asn Lys Thr Gln Gln Gln Met Thr Ile Arg Phe Met Leu Gly225 230 235 240aaa ttg gcc ttt ctc atg tat cca aag agt ttt caa aat agt att caa 768Lys Leu Ala Phe Leu Met Tyr Pro Lys Ser Phe Gln Asn Ser Ile Gln 245 250 255atg caa aag gac ttt gtt gat gaa tat atc gac aga gta gta ggt atg 816Met Gln Lys Asp Phe Val Asp Glu Tyr Ile Asp Arg Val Val Gly Met 260 265 270tcc gaa gaa gaa ttg aac aat cat cca aag agc tat gtt ttg ttg tac 864Ser Glu Glu Glu Leu Asn Asn His Pro Lys Ser Tyr Val Leu Leu Tyr 275 280 285caa tta gca aga caa act aag aat cgt gat ata tta caa gat gaa ttg 912Gln Leu Ala Arg Gln Thr Lys Asn Arg Asp Ile Leu Gln Asp Glu Leu 290 295 300atg tcc att tta ctt gca ggt aga gac acc act gcc agt ttg ttg act 960Met Ser Ile Leu Leu Ala Gly Arg Asp Thr Thr Ala Ser Leu Leu Thr305 310 315 320ttt ttg ttt ttc gaa tta agt cac cat cca gaa gta ttt aac aaa tta 1008Phe Leu Phe Phe Glu Leu Ser His His Pro Glu Val Phe Asn Lys Leu 325 330 335aaa gag gaa atc gaa aga cac ttt cct gat gtt gaa tcc gtt aca ttt 1056Lys Glu Glu Ile Glu Arg His Phe Pro Asp Val Glu Ser Val Thr Phe 340 345 350gga act atc cag aga tgc gac tat ctt caa tgg tgt att aac gaa act 1104Gly Thr Ile Gln Arg Cys Asp Tyr Leu Gln Trp Cys Ile Asn Glu Thr 355 360 365atg aga ctc cat cca tca gtt cct ttt aat ttc aga act gca gcc aat 1152Met Arg Leu His Pro Ser Val Pro Phe Asn Phe Arg Thr Ala Ala Asn 370 375 380gac aca gta ata cca aga ggt gga ggt aaa tcc tgt aca gat cct att 1200Asp Thr Val Ile Pro Arg Gly Gly Gly Lys Ser Cys Thr Asp Pro Ile385 390 395 400ctt gtc cat aag ggt gaa caa gta tta ttc agt ttc tat tct gta aac 1248Leu Val His Lys Gly Glu Gln Val Leu Phe Ser Phe Tyr Ser Val Asn 405 410 415aga gaa gaa aag tat ttt ggt aca aat acc gac aag ttt gct cca gaa 1296Arg Glu Glu Lys Tyr Phe Gly Thr Asn Thr Asp Lys Phe Ala Pro Glu 420 425 430aga tgg agt gaa tca tta agg aga act gag ttc ata cca ttt tct gct 1344Arg Trp Ser Glu Ser Leu Arg Arg Thr Glu Phe Ile Pro Phe Ser Ala 435 440 445gga cct cgt gcc tgt ttg ggt caa cag tta gct aga gtt gaa gct tca 1392Gly Pro Arg Ala Cys Leu Gly Gln Gln Leu Ala Arg Val Glu Ala Ser 450 455 460tat gtt act att aga ttg ctt caa acc ttt cat ggg ttg cat aat gcc 1440Tyr Val Thr Ile Arg Leu Leu Gln Thr Phe His Gly Leu His Asn Ala465 470 475 480agt aaa caa tac cca cca aat aga gtg gtt gca gct aca atg aga ttg 1488Ser Lys Gln Tyr Pro Pro Asn Arg Val Val Ala Ala Thr Met Arg Leu 485 490 495act gac ggt tgt aac gtt tgt ttt atc tag 1518Thr Asp Gly Cys Asn Val Cys Phe Ile 500 50548505PRTCandida tropicalis 48Met Tyr Gln Leu Phe Cys Phe Leu Ala Gly Ile Ile Val Val Tyr Lys1 5 10 15Ala Ala Gln Tyr Tyr Lys Arg Arg Thr Leu Val Thr Lys Phe His Cys 20 25 30Lys Gln Ala Arg Ile Ser Pro Asn Lys Ser Trp Leu Glu Tyr Leu Gly 35 40 45Ile Ala Ser Val Val His Ala Asn Glu Met Ile Arg Lys Gly Gly Leu 50 55 60Tyr Ser Glu Ile Asp Gly Arg Phe Lys Ser Leu Asp Val Ser Thr Phe65 70 75 80Lys Ser Ile Thr Leu Gly Lys Thr Thr Tyr Val Thr Lys Asp Ile Glu 85 90 95Asn Ile Arg His Ile Leu Ser Ala Thr Glu Met Asn Ser Trp Asn Leu 100 105 110Gly Ala Arg Pro Ile Ala Leu Arg Pro Leu Ile Gly Asp Gly Ile Phe 115 120 125Ala Ser Glu Gly Gln Ser Trp Lys His Ser Arg Ile Met Leu Arg Pro 130 135 140Val Phe Ala Lys Glu His Val Lys Gln Ile Thr Ser Met Glu Pro Tyr145 150 155 160Val Gln Leu Leu Ile Lys Ile Ile Lys Asn His Glu Gly Glu Pro Leu 165 170 175Glu Phe Gln Thr Leu Ala His Leu Phe Thr Ile Asp Tyr Ser Thr Asp 180 185 190Phe Leu Leu Gly Glu Ser Cys Asp Ser Leu Lys Asp Phe Leu Gly Glu 195 200 205Glu Ser Asn Ser Thr Leu Asp Thr Ser Leu Arg Leu Ala Phe Ala Ser 210 215 220Gln Phe Asn Lys Thr Gln Gln Gln Met Thr Ile Arg Phe Met Leu Gly225 230 235 240Lys Leu Ala Phe Leu Met Tyr Pro Lys Ser Phe Gln Asn Ser Ile Gln 245 250 255Met Gln Lys Asp Phe Val Asp Glu Tyr Ile Asp Arg Val Val Gly Met 260 265 270Ser Glu Glu Glu Leu Asn Asn His Pro Lys Ser Tyr Val Leu Leu Tyr 275 280 285Gln Leu Ala Arg Gln Thr Lys Asn Arg Asp Ile Leu Gln Asp Glu Leu 290 295 300Met Ser Ile Leu Leu Ala Gly Arg Asp Thr Thr Ala Ser Leu Leu Thr305 310 315 320Phe Leu Phe Phe Glu Leu Ser His His Pro Glu Val Phe Asn Lys Leu 325 330 335Lys Glu Glu Ile Glu Arg His Phe Pro Asp Val Glu Ser Val Thr Phe 340 345 350Gly Thr Ile Gln Arg Cys Asp Tyr Leu Gln Trp Cys Ile Asn Glu Thr 355 360 365Met Arg Leu His Pro Ser Val Pro Phe Asn Phe Arg Thr Ala Ala Asn 370 375 380Asp Thr Val Ile Pro Arg Gly Gly Gly Lys Ser Cys Thr Asp Pro Ile385 390 395 400Leu Val His Lys Gly Glu Gln Val Leu Phe Ser Phe Tyr Ser Val Asn 405 410 415Arg Glu Glu Lys Tyr Phe Gly Thr Asn Thr Asp Lys Phe Ala Pro Glu 420 425 430Arg Trp Ser Glu Ser Leu Arg Arg Thr Glu Phe Ile Pro Phe Ser Ala 435 440 445Gly Pro Arg Ala Cys Leu Gly Gln Gln Leu Ala Arg Val Glu Ala Ser 450 455 460Tyr Val Thr Ile Arg Leu Leu Gln Thr Phe His Gly Leu His Asn Ala465 470 475 480Ser Lys Gln Tyr Pro Pro Asn Arg Val Val Ala Ala Thr Met Arg Leu 485 490 495Thr Asp Gly Cys Asn Val Cys Phe Ile 500 505491584DNACandida tropicalisCDS(1)..(1581) 49atg agt gtc aat act act tct cca gtt gtg gat agt att agt gat aat 48Met Ser Val Asn Thr Thr Ser Pro Val Val Asp Ser Ile Ser Asp Asn1 5 10 15gct act aca tat ttg agt aca aaa tat atc atc gat agt tta tat tca 96Ala Thr Thr Tyr Leu Ser Thr Lys Tyr Ile Ile Asp Ser Leu Tyr Ser 20 25 30act tat caa caa gct tca tgg tta caa atc att tta acc tca atc att 144Thr Tyr Gln Gln Ala Ser Trp Leu Gln Ile Ile Leu Thr Ser Ile Ile 35 40 45ctc att ttg act tat gat caa atc ctg tat caa atc aat aaa ggt tca 192Leu Ile Leu Thr Tyr Asp Gln Ile Leu Tyr Gln Ile Asn Lys Gly Ser 50 55 60att gct ggt cca aaa ttc aaa ttt tgg cca att att ggt cca ttc ttg 240Ile Ala Gly Pro Lys Phe Lys Phe Trp Pro Ile Ile Gly Pro Phe Leu65 70 75 80gaa tct tta gat cca aaa ttc gaa gaa tat aaa gct aaa tgg gat tct 288Glu Ser Leu Asp Pro Lys Phe Glu Glu Tyr Lys Ala Lys Trp Asp Ser 85 90 95ggt gaa ttg agt tgt gtt tcc att ttc cat aaa ttc gtt gtt att gct 336Gly Glu Leu Ser Cys Val Ser Ile Phe His Lys Phe Val Val Ile Ala 100 105 110tct tct cgt gat tta gct aga aag att tta gca tct cca aaa tac gtt 384Ser Ser Arg Asp Leu Ala Arg Lys Ile Leu Ala Ser Pro Lys Tyr Val 115 120 125aaa cct tgt gtt gtt gat gtt gct gtt aaa att tta aga cct tca aat 432Lys Pro Cys Val Val Asp Val Ala Val Lys Ile Leu Arg Pro Ser Asn 130 135 140tgg gtt ttc tta gac ggt aaa gca cat act gat tac cgt cgt tct ttg 480Trp Val Phe Leu Asp Gly Lys Ala His Thr Asp Tyr Arg Arg Ser Leu145 150 155 160aat ggt tta ttc tcc caa aga gct ttg gaa att tac att cca gtt caa 528Asn Gly Leu Phe Ser Gln Arg Ala Leu Glu Ile Tyr Ile Pro Val Gln 165 170 175gaa aaa tac atg gat att tat tta gat aga ttc tgt aag tac gac ggt 576Glu Lys Tyr Met Asp Ile Tyr Leu Asp Arg Phe Cys Lys Tyr Asp Gly 180 185 190cca cgt gaa ttc ttc cca gaa ttt aga gaa ttg ttg tgt gct tta tct 624Pro Arg Glu Phe Phe Pro Glu Phe Arg Glu Leu Leu Cys Ala Leu Ser 195 200 205ttg aga act ttc tgt ggg gat tac atc act gaa gat caa att gct tta 672Leu Arg Thr Phe Cys Gly Asp Tyr Ile Thr Glu Asp Gln Ile Ala Leu 210 215 220gtt gct gat aac tat tac aga gtc act gct gct ttg gaa ttg gtc aat 720Val Ala Asp Asn Tyr Tyr Arg Val Thr Ala Ala Leu Glu Leu Val Asn225 230 235 240ttc cca atc att att cct tac act aaa act tgg tac ggt aag aag att 768Phe Pro Ile Ile Ile Pro Tyr Thr Lys Thr Trp Tyr Gly Lys Lys Ile 245 250 255gct gat gat acc atg aag att ttt gaa aat tgt gct gct atg tcc aag 816Ala Asp Asp Thr Met Lys Ile Phe Glu Asn Cys Ala Ala Met Ser Lys 260 265 270aaa cac att aat gaa aat aat ggt act cca ggt tgt gtt atg gat gaa 864Lys His Ile Asn Glu Asn Asn Gly Thr Pro Gly Cys Val Met Asp Glu 275 280 285tgg att tac ttg atg aaa gaa gct aaa gaa aaa cac tct gat gat cca 912Trp Ile Tyr Leu Met Lys Glu Ala Lys Glu Lys His Ser Asp Asp Pro 290 295 300gat tcc aaa tta ttg att aga gaa ttc tcc aac cgt gaa att tcc gaa 960Asp Ser Lys Leu Leu Ile Arg Glu Phe Ser Asn Arg Glu Ile Ser Glu305 310 315 320gcc att ttc act ttc ttg ttt gct tct caa gat gcc tct tct tct ttg 1008Ala Ile Phe Thr Phe Leu Phe Ala Ser Gln Asp Ala Ser Ser Ser Leu 325 330 335gct tgt tgg tta ttc caa att gtc gcc gac aga cca gat gtt gtt gcc 1056Ala Cys Trp Leu Phe Gln Ile Val Ala Asp Arg Pro Asp Val Val Ala 340 345 350aag att aga gaa gaa caa ttg aga gtt aga aac aat gac cca tct gtt 1104Lys Ile Arg Glu Glu Gln Leu Arg Val Arg Asn Asn Asp Pro Ser Val 355 360 365aaa ttg tcc ttg gat ttg att aat gaa atg act tac acc aat gat gtt 1152Lys Leu Ser Leu Asp Leu Ile Asn Glu Met Thr Tyr Thr Asn Asp Val 370 375 380gtt aaa gaa tct ttg aga tac cgt cca cca gtc ttg atg gtt cca tat 1200Val Lys Glu Ser Leu Arg Tyr Arg Pro Pro Val Leu Met Val Pro Tyr385 390 395 400gtt gtt aag aaa gct ttc cca gtt act gaa aaa tac act gct cca aag 1248Val Val Lys Lys Ala Phe Pro Val Thr Glu Lys Tyr Thr Ala Pro Lys 405 410 415ggt tct atg ctt atc cca act ttg tac cct gct tta cat gat cct gaa 1296Gly Ser Met Leu Ile Pro Thr Leu Tyr Pro Ala Leu His Asp Pro Glu 420 425 430gtt tat gat gaa cca gat tct ttc att cca gaa aga tgg gcc act gct 1344Val Tyr Asp Glu Pro Asp Ser Phe Ile Pro Glu Arg Trp Ala Thr Ala 435 440 445tct ggt gat atg tac aaa cgt aac tgg ttg gtc ttc ggt act ggt cca 1392Ser Gly Asp Met Tyr Lys Arg Asn Trp Leu Val Phe Gly Thr Gly Pro 450 455 460cac gtt tgt ttg ggt aag aac tat gtc atg atg ttg ttt act ggt atg 1440His Val Cys Leu Gly Lys Asn Tyr Val Met Met Leu Phe Thr Gly Met465 470 475 480ttg ggt aaa ttt gtc atg aac tct gat atc att cat cac aaa act gca 1488Leu Gly Lys Phe Val Met Asn Ser Asp Ile Ile His His Lys Thr Ala 485 490 495tta tct gaa gaa atc aaa gtt ttc gct act att ttc cct aag gac gat 1536Leu Ser Glu Glu Ile Lys Val Phe Ala Thr Ile Phe Pro Lys Asp Asp 500 505 510gtt att tta gaa tgg aaa aag aga gat ccg tta gct gct tct aat taa 1584Val Ile Leu Glu Trp Lys Lys Arg Asp Pro Leu Ala Ala Ser Asn 515 520 52550527PRTCandida tropicalis 50Met Ser Val Asn Thr Thr Ser Pro Val Val Asp Ser Ile Ser Asp Asn1 5 10 15Ala Thr Thr Tyr Leu Ser Thr Lys Tyr Ile Ile Asp Ser Leu Tyr Ser 20 25 30Thr Tyr Gln Gln Ala Ser Trp Leu Gln Ile Ile Leu Thr Ser Ile Ile 35 40 45Leu Ile Leu Thr Tyr Asp Gln Ile Leu Tyr Gln Ile Asn Lys Gly Ser 50 55 60Ile Ala Gly Pro Lys Phe Lys Phe Trp Pro Ile Ile Gly Pro Phe Leu65 70 75 80Glu Ser Leu Asp Pro Lys Phe Glu Glu Tyr Lys Ala Lys Trp Asp Ser 85 90 95Gly Glu Leu Ser Cys Val Ser Ile Phe His Lys Phe Val Val Ile Ala 100 105 110Ser Ser Arg Asp Leu Ala Arg Lys Ile Leu Ala Ser Pro Lys Tyr Val 115 120 125Lys Pro Cys Val Val Asp Val Ala Val Lys Ile Leu Arg Pro Ser Asn 130 135 140Trp Val Phe Leu Asp Gly Lys Ala His Thr Asp Tyr Arg Arg Ser Leu145 150 155 160Asn Gly Leu Phe Ser Gln Arg Ala Leu Glu Ile Tyr Ile Pro Val Gln 165 170 175Glu Lys Tyr Met Asp Ile Tyr Leu Asp Arg Phe Cys Lys Tyr Asp Gly 180 185 190Pro Arg Glu Phe Phe Pro Glu Phe Arg Glu Leu Leu Cys Ala Leu Ser 195 200 205Leu Arg Thr Phe Cys Gly Asp Tyr Ile Thr Glu Asp Gln Ile Ala Leu 210 215 220Val Ala Asp Asn Tyr Tyr Arg Val Thr Ala Ala Leu Glu Leu Val Asn225 230 235 240Phe Pro Ile Ile Ile Pro Tyr Thr Lys Thr Trp Tyr Gly Lys Lys Ile 245 250 255Ala Asp Asp Thr Met Lys Ile Phe Glu Asn Cys Ala Ala Met Ser Lys 260 265 270Lys His Ile Asn Glu Asn Asn Gly Thr Pro Gly Cys Val Met Asp Glu 275 280 285Trp Ile Tyr Leu Met Lys Glu Ala Lys Glu Lys His Ser Asp Asp Pro 290 295 300Asp Ser Lys Leu Leu Ile Arg Glu Phe Ser Asn Arg Glu Ile Ser Glu305 310 315 320Ala Ile Phe Thr Phe Leu Phe Ala Ser Gln Asp Ala Ser Ser Ser Leu 325 330 335Ala Cys Trp Leu Phe Gln Ile Val Ala Asp Arg Pro Asp Val Val Ala 340 345 350Lys Ile Arg Glu Glu Gln Leu Arg Val Arg Asn Asn Asp Pro Ser Val 355 360 365Lys Leu Ser Leu Asp Leu Ile Asn Glu Met Thr Tyr Thr Asn Asp Val 370 375 380Val Lys Glu Ser Leu Arg Tyr Arg Pro Pro Val Leu Met Val Pro Tyr385 390 395 400Val Val Lys Lys Ala Phe Pro Val Thr Glu Lys Tyr Thr Ala Pro Lys 405 410 415Gly Ser Met Leu Ile Pro Thr Leu Tyr Pro Ala Leu His Asp Pro Glu 420

425 430Val Tyr Asp Glu Pro Asp Ser Phe Ile Pro Glu Arg Trp Ala Thr Ala 435 440 445Ser Gly Asp Met Tyr Lys Arg Asn Trp Leu Val Phe Gly Thr Gly Pro 450 455 460His Val Cys Leu Gly Lys Asn Tyr Val Met Met Leu Phe Thr Gly Met465 470 475 480Leu Gly Lys Phe Val Met Asn Ser Asp Ile Ile His His Lys Thr Ala 485 490 495Leu Ser Glu Glu Ile Lys Val Phe Ala Thr Ile Phe Pro Lys Asp Asp 500 505 510Val Ile Leu Glu Trp Lys Lys Arg Asp Pro Leu Ala Ala Ser Asn 515 520 525511587DNACandida tropicalisCDS(1)..(1584) 51atg gct att gtt gat act gcc att gat ggc atc aat tat ttc tta tcc 48Met Ala Ile Val Asp Thr Ala Ile Asp Gly Ile Asn Tyr Phe Leu Ser1 5 10 15tta tca tta act caa caa atc acc atc ttg gtt gtt ttc cca ttc atc 96Leu Ser Leu Thr Gln Gln Ile Thr Ile Leu Val Val Phe Pro Phe Ile 20 25 30tac aac ata gca tgg caa tta ctt tac tcc tta aga aaa gat aga gtt 144Tyr Asn Ile Ala Trp Gln Leu Leu Tyr Ser Leu Arg Lys Asp Arg Val 35 40 45cca atg gtt ttc tac tgg atc cca tgg ttt ggt tct gct gct agt tat 192Pro Met Val Phe Tyr Trp Ile Pro Trp Phe Gly Ser Ala Ala Ser Tyr 50 55 60ggt atg caa cca tac gaa ttc ttt gaa aag tgc aga ttg aaa tat ggt 240Gly Met Gln Pro Tyr Glu Phe Phe Glu Lys Cys Arg Leu Lys Tyr Gly65 70 75 80gat gtt ttt tca ttt atg tta tta ggt aaa gtt atg act gtt tat ttg 288Asp Val Phe Ser Phe Met Leu Leu Gly Lys Val Met Thr Val Tyr Leu 85 90 95ggt cca aaa ggt cac gaa ttc att tac aat gct aaa tta tcc gat gtt 336Gly Pro Lys Gly His Glu Phe Ile Tyr Asn Ala Lys Leu Ser Asp Val 100 105 110tct gct gaa gaa gct tat acc cat ttg act act cct gtt ttt ggt aaa 384Ser Ala Glu Glu Ala Tyr Thr His Leu Thr Thr Pro Val Phe Gly Lys 115 120 125ggt gtt att tat gat tgt cca aac tct aga tta atg gaa caa aag aag 432Gly Val Ile Tyr Asp Cys Pro Asn Ser Arg Leu Met Glu Gln Lys Lys 130 135 140ttt gct aaa ttt gct ttg act act gat tct ttc aaa acc tat gtt cca 480Phe Ala Lys Phe Ala Leu Thr Thr Asp Ser Phe Lys Thr Tyr Val Pro145 150 155 160aag atc aga gaa gaa gtt ttg aat tat ttt gtt aac gat gtt agt ttc 528Lys Ile Arg Glu Glu Val Leu Asn Tyr Phe Val Asn Asp Val Ser Phe 165 170 175aaa acc aag gaa aga gac cat ggt gtt gct agt gtt atg aaa act caa 576Lys Thr Lys Glu Arg Asp His Gly Val Ala Ser Val Met Lys Thr Gln 180 185 190cca gaa atc act att ttc act gct tct cgt tgt tta ttt ggt gat gaa 624Pro Glu Ile Thr Ile Phe Thr Ala Ser Arg Cys Leu Phe Gly Asp Glu 195 200 205atg aga aag agt ttc gac aga tca ttt gct caa ttg tat gct gac ttg 672Met Arg Lys Ser Phe Asp Arg Ser Phe Ala Gln Leu Tyr Ala Asp Leu 210 215 220gat aaa ggt ttc acc cca atc aac ttt gtt ttc cca aac ttg cca tta 720Asp Lys Gly Phe Thr Pro Ile Asn Phe Val Phe Pro Asn Leu Pro Leu225 230 235 240cct cat tac tgg aga cgt gac gct gct caa aga aag ata tct gct cat 768Pro His Tyr Trp Arg Arg Asp Ala Ala Gln Arg Lys Ile Ser Ala His 245 250 255tac atg aag gaa att aag aga aga aga gaa agc ggt gat att gat cca 816Tyr Met Lys Glu Ile Lys Arg Arg Arg Glu Ser Gly Asp Ile Asp Pro 260 265 270aag aga gat ttg att gat tcc ttg ttg gtt aac tct act tat aaa gat 864Lys Arg Asp Leu Ile Asp Ser Leu Leu Val Asn Ser Thr Tyr Lys Asp 275 280 285ggt gtt aaa atg act gat caa gaa att gct aac ctt tta att ggt gtt 912Gly Val Lys Met Thr Asp Gln Glu Ile Ala Asn Leu Leu Ile Gly Val 290 295 300ttg atg ggt ggt caa cat act tct gct tcc act tct gcc tgg ttc ttg 960Leu Met Gly Gly Gln His Thr Ser Ala Ser Thr Ser Ala Trp Phe Leu305 310 315 320ttg cat ttg gct gaa caa cca caa tta caa gat gat ctt tac gaa gaa 1008Leu His Leu Ala Glu Gln Pro Gln Leu Gln Asp Asp Leu Tyr Glu Glu 325 330 335ttg acc aac ttg ttg aaa gaa aag ggt ggt gac ttg aac gat ttg act 1056Leu Thr Asn Leu Leu Lys Glu Lys Gly Gly Asp Leu Asn Asp Leu Thr 340 345 350tac gaa gac ttg caa aaa tta cca ttg gtt aac aac act att aaa gaa 1104Tyr Glu Asp Leu Gln Lys Leu Pro Leu Val Asn Asn Thr Ile Lys Glu 355 360 365act ttg aga atg cac atg cca ttg cat tct att ttc aga aaa gtt atg 1152Thr Leu Arg Met His Met Pro Leu His Ser Ile Phe Arg Lys Val Met 370 375 380aac cca ttg aga gtc cca aat acc aaa tat gtt att cca aaa ggt cac 1200Asn Pro Leu Arg Val Pro Asn Thr Lys Tyr Val Ile Pro Lys Gly His385 390 395 400tat gtc tta gtt tct gcc ggt tat gct cat acc agt gat aga tgg ttt 1248Tyr Val Leu Val Ser Ala Gly Tyr Ala His Thr Ser Asp Arg Trp Phe 405 410 415gaa cac cca gaa cat ttc aac cca aga aga tgg gaa tct gat gat acc 1296Glu His Pro Glu His Phe Asn Pro Arg Arg Trp Glu Ser Asp Asp Thr 420 425 430aag gct agt gct gtt tct ttc aat tct gaa gat act gtt gat tat ggt 1344Lys Ala Ser Ala Val Ser Phe Asn Ser Glu Asp Thr Val Asp Tyr Gly 435 440 445ttc ggt aaa att tcc aaa ggt gtc tcc tct cca tac ttg cca ttc ggt 1392Phe Gly Lys Ile Ser Lys Gly Val Ser Ser Pro Tyr Leu Pro Phe Gly 450 455 460ggt ggt aga cac aga tgt att ggt gaa caa ttt gct tat gtt caa ttg 1440Gly Gly Arg His Arg Cys Ile Gly Glu Gln Phe Ala Tyr Val Gln Leu465 470 475 480gga act att ttg acc act tat atc tac aac ttc aaa tgg aga tta aac 1488Gly Thr Ile Leu Thr Thr Tyr Ile Tyr Asn Phe Lys Trp Arg Leu Asn 485 490 495ggt gat aag gtt cca gat gtt gat tac caa tcc atg gtt acc tta cca 1536Gly Asp Lys Val Pro Asp Val Asp Tyr Gln Ser Met Val Thr Leu Pro 500 505 510tta gaa cct gct gaa atc gtt tgg gaa aag aga gat act tgt atg gtt 1584Leu Glu Pro Ala Glu Ile Val Trp Glu Lys Arg Asp Thr Cys Met Val 515 520 525tag 1587 52528PRTCandida tropicalis 52Met Ala Ile Val Asp Thr Ala Ile Asp Gly Ile Asn Tyr Phe Leu Ser1 5 10 15Leu Ser Leu Thr Gln Gln Ile Thr Ile Leu Val Val Phe Pro Phe Ile 20 25 30Tyr Asn Ile Ala Trp Gln Leu Leu Tyr Ser Leu Arg Lys Asp Arg Val 35 40 45Pro Met Val Phe Tyr Trp Ile Pro Trp Phe Gly Ser Ala Ala Ser Tyr 50 55 60Gly Met Gln Pro Tyr Glu Phe Phe Glu Lys Cys Arg Leu Lys Tyr Gly65 70 75 80Asp Val Phe Ser Phe Met Leu Leu Gly Lys Val Met Thr Val Tyr Leu 85 90 95Gly Pro Lys Gly His Glu Phe Ile Tyr Asn Ala Lys Leu Ser Asp Val 100 105 110Ser Ala Glu Glu Ala Tyr Thr His Leu Thr Thr Pro Val Phe Gly Lys 115 120 125Gly Val Ile Tyr Asp Cys Pro Asn Ser Arg Leu Met Glu Gln Lys Lys 130 135 140Phe Ala Lys Phe Ala Leu Thr Thr Asp Ser Phe Lys Thr Tyr Val Pro145 150 155 160Lys Ile Arg Glu Glu Val Leu Asn Tyr Phe Val Asn Asp Val Ser Phe 165 170 175Lys Thr Lys Glu Arg Asp His Gly Val Ala Ser Val Met Lys Thr Gln 180 185 190Pro Glu Ile Thr Ile Phe Thr Ala Ser Arg Cys Leu Phe Gly Asp Glu 195 200 205Met Arg Lys Ser Phe Asp Arg Ser Phe Ala Gln Leu Tyr Ala Asp Leu 210 215 220Asp Lys Gly Phe Thr Pro Ile Asn Phe Val Phe Pro Asn Leu Pro Leu225 230 235 240Pro His Tyr Trp Arg Arg Asp Ala Ala Gln Arg Lys Ile Ser Ala His 245 250 255Tyr Met Lys Glu Ile Lys Arg Arg Arg Glu Ser Gly Asp Ile Asp Pro 260 265 270Lys Arg Asp Leu Ile Asp Ser Leu Leu Val Asn Ser Thr Tyr Lys Asp 275 280 285Gly Val Lys Met Thr Asp Gln Glu Ile Ala Asn Leu Leu Ile Gly Val 290 295 300Leu Met Gly Gly Gln His Thr Ser Ala Ser Thr Ser Ala Trp Phe Leu305 310 315 320Leu His Leu Ala Glu Gln Pro Gln Leu Gln Asp Asp Leu Tyr Glu Glu 325 330 335Leu Thr Asn Leu Leu Lys Glu Lys Gly Gly Asp Leu Asn Asp Leu Thr 340 345 350Tyr Glu Asp Leu Gln Lys Leu Pro Leu Val Asn Asn Thr Ile Lys Glu 355 360 365Thr Leu Arg Met His Met Pro Leu His Ser Ile Phe Arg Lys Val Met 370 375 380Asn Pro Leu Arg Val Pro Asn Thr Lys Tyr Val Ile Pro Lys Gly His385 390 395 400Tyr Val Leu Val Ser Ala Gly Tyr Ala His Thr Ser Asp Arg Trp Phe 405 410 415Glu His Pro Glu His Phe Asn Pro Arg Arg Trp Glu Ser Asp Asp Thr 420 425 430Lys Ala Ser Ala Val Ser Phe Asn Ser Glu Asp Thr Val Asp Tyr Gly 435 440 445Phe Gly Lys Ile Ser Lys Gly Val Ser Ser Pro Tyr Leu Pro Phe Gly 450 455 460Gly Gly Arg His Arg Cys Ile Gly Glu Gln Phe Ala Tyr Val Gln Leu465 470 475 480Gly Thr Ile Leu Thr Thr Tyr Ile Tyr Asn Phe Lys Trp Arg Leu Asn 485 490 495Gly Asp Lys Val Pro Asp Val Asp Tyr Gln Ser Met Val Thr Leu Pro 500 505 510Leu Glu Pro Ala Glu Ile Val Trp Glu Lys Arg Asp Thr Cys Met Val 515 520 525532097DNACandida cloacaeCDS(1)..(2094) 53atg tcc cat caa gtt gaa gac cac gac tta gac gtg ttc tgt tta ttg 48Met Ser His Gln Val Glu Asp His Asp Leu Asp Val Phe Cys Leu Leu1 5 10 15gcc gat gcc gtg ctc cat gaa att cct ccc agc gaa atc gtg gag tac 96Ala Asp Ala Val Leu His Glu Ile Pro Pro Ser Glu Ile Val Glu Tyr 20 25 30ctt cat cct gac ttc ccc aaa gat aag atc gaa gag tat ttg aca ggc 144Leu His Pro Asp Phe Pro Lys Asp Lys Ile Glu Glu Tyr Leu Thr Gly 35 40 45ttt tcc cgt ccg tct gct gtt cct cag ttt aga caa tgt gcc aag aag 192Phe Ser Arg Pro Ser Ala Val Pro Gln Phe Arg Gln Cys Ala Lys Lys 50 55 60ctt atc aac aga ggc tcc gag ctg tcg atc aag ttg ttt ttg tac ttg 240Leu Ile Asn Arg Gly Ser Glu Leu Ser Ile Lys Leu Phe Leu Tyr Leu65 70 75 80acc act gcg ttg gac tca aga atc ctt gca cct gcc ttg acc aat tcg 288Thr Thr Ala Leu Asp Ser Arg Ile Leu Ala Pro Ala Leu Thr Asn Ser 85 90 95ttg act ttg atc agg gat atg gat ctt tcc caa aga gag gag ttg ttg 336Leu Thr Leu Ile Arg Asp Met Asp Leu Ser Gln Arg Glu Glu Leu Leu 100 105 110aga tca tgg aga gac tct cct tta act gca aaa aga aga tta ttt aga 384Arg Ser Trp Arg Asp Ser Pro Leu Thr Ala Lys Arg Arg Leu Phe Arg 115 120 125gtg tat gcc tct ttt acc ttg tct act ttt aac aag ttg gga aca gac 432Val Tyr Ala Ser Phe Thr Leu Ser Thr Phe Asn Lys Leu Gly Thr Asp 130 135 140ttg cac ttt aag gcg ttg ggc tac cca ggt aga gag ctc aga acg caa 480Leu His Phe Lys Ala Leu Gly Tyr Pro Gly Arg Glu Leu Arg Thr Gln145 150 155 160att caa gac tac gaa gtc gac cct ttt aga tat tcg ttt atg gag aaa 528Ile Gln Asp Tyr Glu Val Asp Pro Phe Arg Tyr Ser Phe Met Glu Lys 165 170 175ctt aaa cac gag ggc cac gaa ttg ttc ctt cct gat att gac gtt tta 576Leu Lys His Glu Gly His Glu Leu Phe Leu Pro Asp Ile Asp Val Leu 180 185 190atc atc ggg tcg gga tca gga gca ggt gtg gtt gca caa act ctt act 624Ile Ile Gly Ser Gly Ser Gly Ala Gly Val Val Ala Gln Thr Leu Thr 195 200 205gaa agt ggc ctc aaa tca ttg gtt ttg gaa aag ggc aaa tac ttt gcc 672Glu Ser Gly Leu Lys Ser Leu Val Leu Glu Lys Gly Lys Tyr Phe Ala 210 215 220agt gaa gaa ttg tgc atg acg gac ttg gac ggt aac gag gca tta ttc 720Ser Glu Glu Leu Cys Met Thr Asp Leu Asp Gly Asn Glu Ala Leu Phe225 230 235 240gaa agt gga gga aca att cct tcc acc aac caa caa ttg ttc atg att 768Glu Ser Gly Gly Thr Ile Pro Ser Thr Asn Gln Gln Leu Phe Met Ile 245 250 255gca ggt tcg act ttt ggt ggt ggt tct aca gtt aat tgg tct gcc tgt 816Ala Gly Ser Thr Phe Gly Gly Gly Ser Thr Val Asn Trp Ser Ala Cys 260 265 270ttg aag acc cca ttc aaa gta aga aag gaa tgg tat gac gat ttc gga 864Leu Lys Thr Pro Phe Lys Val Arg Lys Glu Trp Tyr Asp Asp Phe Gly 275 280 285ctt gat ttt gtc gct act caa caa tac gac gat tgt atg gat tac gtg 912Leu Asp Phe Val Ala Thr Gln Gln Tyr Asp Asp Cys Met Asp Tyr Val 290 295 300tgg aag aaa atg ggt gct tcg acc gaa cat atc gaa cat tct gct gca 960Trp Lys Lys Met Gly Ala Ser Thr Glu His Ile Glu His Ser Ala Ala305 310 315 320aat gcc gtc atc atg gac ggg gca gca aaa ctt ggc tac gca cac aga 1008Asn Ala Val Ile Met Asp Gly Ala Ala Lys Leu Gly Tyr Ala His Arg 325 330 335gca ctt gag cag aat acc ggg ggc cat gtt cac gac tgt ggg atg tgc 1056Ala Leu Glu Gln Asn Thr Gly Gly His Val His Asp Cys Gly Met Cys 340 345 350cac ttg gga tgt aga ttc ggt atc aaa caa ggt ggt gta aat tgc tgg 1104His Leu Gly Cys Arg Phe Gly Ile Lys Gln Gly Gly Val Asn Cys Trp 355 360 365ttc cgt gaa cct agt gaa aag ggt tct aag ttc atg gaa caa gtt gtt 1152Phe Arg Glu Pro Ser Glu Lys Gly Ser Lys Phe Met Glu Gln Val Val 370 375 380gtt gaa aag att ttg cag cac aag ggt aaa gct act ggg att ttg tgt 1200Val Glu Lys Ile Leu Gln His Lys Gly Lys Ala Thr Gly Ile Leu Cys385 390 395 400aga gat act gaa agt ggg att aaa ttc aaa atc act gga cca aag aaa 1248Arg Asp Thr Glu Ser Gly Ile Lys Phe Lys Ile Thr Gly Pro Lys Lys 405 410 415tac gtt gtt tcc ggt ggt tct ttg caa acc cca gtt ttg tta caa aaa 1296Tyr Val Val Ser Gly Gly Ser Leu Gln Thr Pro Val Leu Leu Gln Lys 420 425 430tct ggt ttc aag aat aaa cat att gga gct aac tta aaa ctt cac cca 1344Ser Gly Phe Lys Asn Lys His Ile Gly Ala Asn Leu Lys Leu His Pro 435 440 445gtc tcg gtt gcc ctt ggg gac ttt ggt aat gaa gtg gac ttt gaa gcc 1392Val Ser Val Ala Leu Gly Asp Phe Gly Asn Glu Val Asp Phe Glu Ala 450 455 460tac aag aga cca ctt atg acc gcc gtt tgt aat gcc gtc gat gat tta 1440Tyr Lys Arg Pro Leu Met Thr Ala Val Cys Asn Ala Val Asp Asp Leu465 470 475 480gat ggc aag gcc cat gga aca aga att gaa gcc att ttg cat gct cca 1488Asp Gly Lys Ala His Gly Thr Arg Ile Glu Ala Ile Leu His Ala Pro 485 490 495tac gtc act gcc cca ttt tac cca tgg caa tca ggt gct caa gca aga 1536Tyr Val Thr Ala Pro Phe Tyr Pro Trp Gln Ser Gly Ala Gln Ala Arg 500 505 510aag aac ctc ttg aaa tat aaa caa act gtg ccg tta tta ctt ctt tct 1584Lys Asn Leu Leu Lys Tyr Lys Gln Thr Val Pro Leu Leu Leu Leu Ser 515 520 525aga gat aca tca tca ggt acc gtt aca tat gat aaa caa aag cct gac 1632Arg Asp Thr Ser Ser Gly Thr Val Thr Tyr Asp Lys Gln Lys Pro Asp 530 535 540gta ttg gta gtt gac tac act gtt aac aag ttt gac aga aat tcg att 1680Val Leu Val Val Asp Tyr Thr Val Asn Lys Phe Asp Arg Asn Ser Ile545 550 555 560tta caa ggg ttt ttg gtt gct tcc gac atc ttg tat att gaa ggt gct 1728Leu Gln Gly Phe Leu Val Ala Ser Asp Ile Leu Tyr Ile Glu Gly Ala 565 570 575aaa gag att ttg tca cca caa gct tgg gta cca acc ttc aag agc aac 1776Lys Glu Ile Leu Ser Pro Gln Ala Trp Val Pro Thr Phe Lys Ser Asn 580 585 590aaa cca aaa cat gct aga tcg atc aaa gac gaa gat tac gtc aaa tgg 1824Lys Pro Lys His Ala Arg Ser Ile Lys Asp Glu Asp Tyr Val Lys Trp 595 600

605aga gaa acc gtg gcc aag atc cca ttt gac tcc tac ggt tcg cca tac 1872Arg Glu Thr Val Ala Lys Ile Pro Phe Asp Ser Tyr Gly Ser Pro Tyr 610 615 620ggt tct gct cat caa atg agt tcg tgt aga atg tct ggt aag gga cca 1920Gly Ser Ala His Gln Met Ser Ser Cys Arg Met Ser Gly Lys Gly Pro625 630 635 640gga tac ggt gct tgt gac act aaa gga aga tta ttt gaa tgt aac aac 1968Gly Tyr Gly Ala Cys Asp Thr Lys Gly Arg Leu Phe Glu Cys Asn Asn 645 650 655gtt tac gtt gct gat gct tcg gtt atg cct act gca tcg gga gtc aat 2016Val Tyr Val Ala Asp Ala Ser Val Met Pro Thr Ala Ser Gly Val Asn 660 665 670cct atg atc act aca atg gct ttt gca aga cat gtg gcc tta tgt ctt 2064Pro Met Ile Thr Thr Met Ala Phe Ala Arg His Val Ala Leu Cys Leu 675 680 685gct aaa gac ttg caa cca caa act aaa ctt tag 2097Ala Lys Asp Leu Gln Pro Gln Thr Lys Leu 690 69554698PRTCandida cloacae 54Met Ser His Gln Val Glu Asp His Asp Leu Asp Val Phe Cys Leu Leu1 5 10 15Ala Asp Ala Val Leu His Glu Ile Pro Pro Ser Glu Ile Val Glu Tyr 20 25 30Leu His Pro Asp Phe Pro Lys Asp Lys Ile Glu Glu Tyr Leu Thr Gly 35 40 45Phe Ser Arg Pro Ser Ala Val Pro Gln Phe Arg Gln Cys Ala Lys Lys 50 55 60Leu Ile Asn Arg Gly Ser Glu Leu Ser Ile Lys Leu Phe Leu Tyr Leu65 70 75 80Thr Thr Ala Leu Asp Ser Arg Ile Leu Ala Pro Ala Leu Thr Asn Ser 85 90 95Leu Thr Leu Ile Arg Asp Met Asp Leu Ser Gln Arg Glu Glu Leu Leu 100 105 110Arg Ser Trp Arg Asp Ser Pro Leu Thr Ala Lys Arg Arg Leu Phe Arg 115 120 125Val Tyr Ala Ser Phe Thr Leu Ser Thr Phe Asn Lys Leu Gly Thr Asp 130 135 140Leu His Phe Lys Ala Leu Gly Tyr Pro Gly Arg Glu Leu Arg Thr Gln145 150 155 160Ile Gln Asp Tyr Glu Val Asp Pro Phe Arg Tyr Ser Phe Met Glu Lys 165 170 175Leu Lys His Glu Gly His Glu Leu Phe Leu Pro Asp Ile Asp Val Leu 180 185 190Ile Ile Gly Ser Gly Ser Gly Ala Gly Val Val Ala Gln Thr Leu Thr 195 200 205Glu Ser Gly Leu Lys Ser Leu Val Leu Glu Lys Gly Lys Tyr Phe Ala 210 215 220Ser Glu Glu Leu Cys Met Thr Asp Leu Asp Gly Asn Glu Ala Leu Phe225 230 235 240Glu Ser Gly Gly Thr Ile Pro Ser Thr Asn Gln Gln Leu Phe Met Ile 245 250 255Ala Gly Ser Thr Phe Gly Gly Gly Ser Thr Val Asn Trp Ser Ala Cys 260 265 270Leu Lys Thr Pro Phe Lys Val Arg Lys Glu Trp Tyr Asp Asp Phe Gly 275 280 285Leu Asp Phe Val Ala Thr Gln Gln Tyr Asp Asp Cys Met Asp Tyr Val 290 295 300Trp Lys Lys Met Gly Ala Ser Thr Glu His Ile Glu His Ser Ala Ala305 310 315 320Asn Ala Val Ile Met Asp Gly Ala Ala Lys Leu Gly Tyr Ala His Arg 325 330 335Ala Leu Glu Gln Asn Thr Gly Gly His Val His Asp Cys Gly Met Cys 340 345 350His Leu Gly Cys Arg Phe Gly Ile Lys Gln Gly Gly Val Asn Cys Trp 355 360 365Phe Arg Glu Pro Ser Glu Lys Gly Ser Lys Phe Met Glu Gln Val Val 370 375 380Val Glu Lys Ile Leu Gln His Lys Gly Lys Ala Thr Gly Ile Leu Cys385 390 395 400Arg Asp Thr Glu Ser Gly Ile Lys Phe Lys Ile Thr Gly Pro Lys Lys 405 410 415Tyr Val Val Ser Gly Gly Ser Leu Gln Thr Pro Val Leu Leu Gln Lys 420 425 430Ser Gly Phe Lys Asn Lys His Ile Gly Ala Asn Leu Lys Leu His Pro 435 440 445Val Ser Val Ala Leu Gly Asp Phe Gly Asn Glu Val Asp Phe Glu Ala 450 455 460Tyr Lys Arg Pro Leu Met Thr Ala Val Cys Asn Ala Val Asp Asp Leu465 470 475 480Asp Gly Lys Ala His Gly Thr Arg Ile Glu Ala Ile Leu His Ala Pro 485 490 495Tyr Val Thr Ala Pro Phe Tyr Pro Trp Gln Ser Gly Ala Gln Ala Arg 500 505 510Lys Asn Leu Leu Lys Tyr Lys Gln Thr Val Pro Leu Leu Leu Leu Ser 515 520 525Arg Asp Thr Ser Ser Gly Thr Val Thr Tyr Asp Lys Gln Lys Pro Asp 530 535 540Val Leu Val Val Asp Tyr Thr Val Asn Lys Phe Asp Arg Asn Ser Ile545 550 555 560Leu Gln Gly Phe Leu Val Ala Ser Asp Ile Leu Tyr Ile Glu Gly Ala 565 570 575Lys Glu Ile Leu Ser Pro Gln Ala Trp Val Pro Thr Phe Lys Ser Asn 580 585 590Lys Pro Lys His Ala Arg Ser Ile Lys Asp Glu Asp Tyr Val Lys Trp 595 600 605Arg Glu Thr Val Ala Lys Ile Pro Phe Asp Ser Tyr Gly Ser Pro Tyr 610 615 620Gly Ser Ala His Gln Met Ser Ser Cys Arg Met Ser Gly Lys Gly Pro625 630 635 640Gly Tyr Gly Ala Cys Asp Thr Lys Gly Arg Leu Phe Glu Cys Asn Asn 645 650 655Val Tyr Val Ala Asp Ala Ser Val Met Pro Thr Ala Ser Gly Val Asn 660 665 670Pro Met Ile Thr Thr Met Ala Phe Ala Arg His Val Ala Leu Cys Leu 675 680 685Ala Lys Asp Leu Gln Pro Gln Thr Lys Leu 690 695552097DNACandida cloacaeCDS(1)..(2094) 55atg aat ccc gtt gtt gaa gac agc cat tta gat gtg ttc tgc ttg tta 48Met Asn Pro Val Val Glu Asp Ser His Leu Asp Val Phe Cys Leu Leu1 5 10 15gcc gat gct gtg gtc cat gag ata cct cct agt gag atc gta gag tac 96Ala Asp Ala Val Val His Glu Ile Pro Pro Ser Glu Ile Val Glu Tyr 20 25 30tta cat cct gat ttc cca aag gac aag gtt gaa gag tat ttg gct gag 144Leu His Pro Asp Phe Pro Lys Asp Lys Val Glu Glu Tyr Leu Ala Glu 35 40 45ttt tct cat cct tca gca att ccc gaa ttt aga gaa gtt gca aaa aga 192Phe Ser His Pro Ser Ala Ile Pro Glu Phe Arg Glu Val Ala Lys Arg 50 55 60att att aac aaa ggg act gtg ctg tca ata aag ttg ttt ttg ctc ttg 240Ile Ile Asn Lys Gly Thr Val Leu Ser Ile Lys Leu Phe Leu Leu Leu65 70 75 80gca act gct cta gac tcg aga atc ctt gct cct gcg ttg acc aac tcg 288Ala Thr Ala Leu Asp Ser Arg Ile Leu Ala Pro Ala Leu Thr Asn Ser 85 90 95acg acg tta atc aga gat atg gat ctt tct caa aga gaa gaa tta ttg 336Thr Thr Leu Ile Arg Asp Met Asp Leu Ser Gln Arg Glu Glu Leu Leu 100 105 110aga tca tgg aga gac tct cca ttc act aca aaa agg aaa ttg ttc agg 384Arg Ser Trp Arg Asp Ser Pro Phe Thr Thr Lys Arg Lys Leu Phe Arg 115 120 125gtg tat aat tca ttc acc ttg aac gcg ttt agt aag acc gca aca gat 432Val Tyr Asn Ser Phe Thr Leu Asn Ala Phe Ser Lys Thr Ala Thr Asp 130 135 140ttg cac ttc aaa gcg ttg gga tat cct ggt aga gag ctc agg act caa 480Leu His Phe Lys Ala Leu Gly Tyr Pro Gly Arg Glu Leu Arg Thr Gln145 150 155 160att cag gac tat gag gtc gat cct ttc aga tat acg ttc ttg gaa aaa 528Ile Gln Asp Tyr Glu Val Asp Pro Phe Arg Tyr Thr Phe Leu Glu Lys 165 170 175ccc caa caa gac ggc cag gag tta cat ttt ccc gac att gat gtc ttg 576Pro Gln Gln Asp Gly Gln Glu Leu His Phe Pro Asp Ile Asp Val Leu 180 185 190att atc ggg tct ggt tcg gga gca gga gtg gtt gct caa act ctt tcg 624Ile Ile Gly Ser Gly Ser Gly Ala Gly Val Val Ala Gln Thr Leu Ser 195 200 205gaa aac gga ctt aaa tca ttg gtg ttg gaa aaa ggt aaa tac ttt tcc 672Glu Asn Gly Leu Lys Ser Leu Val Leu Glu Lys Gly Lys Tyr Phe Ser 210 215 220aat gat gag ttg acc atg aat gat ttg gaa ggt agt gag gca tta ttc 720Asn Asp Glu Leu Thr Met Asn Asp Leu Glu Gly Ser Glu Ala Leu Phe225 230 235 240gaa aat gga ggt gcc ctc agt agt acc aac caa cag ata ttt ata att 768Glu Asn Gly Gly Ala Leu Ser Ser Thr Asn Gln Gln Ile Phe Ile Ile 245 250 255gca ggt tcg act ttt ggg ggt ggt tct aca gtt aat tgg tct gcc tgt 816Ala Gly Ser Thr Phe Gly Gly Gly Ser Thr Val Asn Trp Ser Ala Cys 260 265 270tta aaa act ccg ttc aaa gta aga aaa gag tgg tat gac aac ttc gga 864Leu Lys Thr Pro Phe Lys Val Arg Lys Glu Trp Tyr Asp Asn Phe Gly 275 280 285ctt gat ttc gtc gca acc caa tac tac gaa gat tgt atg gat tat gtt 912Leu Asp Phe Val Ala Thr Gln Tyr Tyr Glu Asp Cys Met Asp Tyr Val 290 295 300tgg aag aaa atg ggt gct tcg aac gaa aat atc gac cat tct ggt gct 960Trp Lys Lys Met Gly Ala Ser Asn Glu Asn Ile Asp His Ser Gly Ala305 310 315 320aat agt gtt ata ttg gaa ggg tcc aaa aaa ctt ggc tac cct cac agg 1008Asn Ser Val Ile Leu Glu Gly Ser Lys Lys Leu Gly Tyr Pro His Arg 325 330 335gcc gtt gaa caa aat aat ggg ggc aaa att cat gac tgt ggt atg tgt 1056Ala Val Glu Gln Asn Asn Gly Gly Lys Ile His Asp Cys Gly Met Cys 340 345 350cac ttg ggt tgt aga ttt ggt att aaa cag gga agt gta aat tgc tgg 1104His Leu Gly Cys Arg Phe Gly Ile Lys Gln Gly Ser Val Asn Cys Trp 355 360 365ttc cgt ggt cca agt gaa aac gga tcc aag ttt atg caa caa gtt ctc 1152Phe Arg Gly Pro Ser Glu Asn Gly Ser Lys Phe Met Gln Gln Val Leu 370 375 380gtg gat aag ata ttg caa cgt gac ggt aaa gca gtc ggt gtt ttg tgt 1200Val Asp Lys Ile Leu Gln Arg Asp Gly Lys Ala Val Gly Val Leu Cys385 390 395 400aga gat gtt gta acc ggt gtt aag ttc aag atc act gga cca aag aaa 1248Arg Asp Val Val Thr Gly Val Lys Phe Lys Ile Thr Gly Pro Lys Lys 405 410 415att gtt gtt ttc tgg tgg ttc ttt gca aac tcc ggt ttt gtt aca aaa 1296Ile Val Val Phe Trp Trp Phe Phe Ala Asn Ser Gly Phe Val Thr Lys 420 425 430tca ggt ttc aag aat aaa cac att ggt gct aac ttg aag ctc cat cca 1344Ser Gly Phe Lys Asn Lys His Ile Gly Ala Asn Leu Lys Leu His Pro 435 440 445gtt tca ctt acg ctt gga gac ttc ggt aac aac gtg gat ttc gaa gct 1392Val Ser Leu Thr Leu Gly Asp Phe Gly Asn Asn Val Asp Phe Glu Ala 450 455 460tac agg aaa cca att atg aca tca att tgt aat aaa gtc gaa gat tta 1440Tyr Arg Lys Pro Ile Met Thr Ser Ile Cys Asn Lys Val Glu Asp Leu465 470 475 480gat gga aag gcc cat ggt aca aga att gaa gct atg ttg aat gct cca 1488Asp Gly Lys Ala His Gly Thr Arg Ile Glu Ala Met Leu Asn Ala Pro 485 490 495tat ggt gtt gca cca ttt ttc ccc tgg aaa tca ggc gct gaa tca aga 1536Tyr Gly Val Ala Pro Phe Phe Pro Trp Lys Ser Gly Ala Glu Ser Arg 500 505 510aag gat ctc ttg aga tac aaa caa act gtg cct ata tta ctt ctt tcc 1584Lys Asp Leu Leu Arg Tyr Lys Gln Thr Val Pro Ile Leu Leu Leu Ser 515 520 525aga gat act act tca ggg tct gtc aca tat gat aaa caa aaa cca gat 1632Arg Asp Thr Thr Ser Gly Ser Val Thr Tyr Asp Lys Gln Lys Pro Asp 530 535 540gca ttg gtg att gat tac ctg tta aac aag ttt gac aga aac tca att 1680Ala Leu Val Ile Asp Tyr Leu Leu Asn Lys Phe Asp Arg Asn Ser Ile545 550 555 560tta caa ggg ttt ttg ata gct tca gac ctt tta tat att gaa ggt gcc 1728Leu Gln Gly Phe Leu Ile Ala Ser Asp Leu Leu Tyr Ile Glu Gly Ala 565 570 575agt aga gat cat gtc acc tac aag ctt ggg tac caa tgg ttc aag agt 1776Ser Arg Asp His Val Thr Tyr Lys Leu Gly Tyr Gln Trp Phe Lys Ser 580 585 590tct aaa cct aaa cat gcc aga tcg att gaa gat gaa gac tac gta aac 1824Ser Lys Pro Lys His Ala Arg Ser Ile Glu Asp Glu Asp Tyr Val Asn 595 600 605tgg aga gct aaa gtt gca aag att cca ttt gat tcc tac gga tca cca 1872Trp Arg Ala Lys Val Ala Lys Ile Pro Phe Asp Ser Tyr Gly Ser Pro 610 615 620tac ggt tca gct cac caa atg agt aca tgc aga atg tca ggt aag gga 1920Tyr Gly Ser Ala His Gln Met Ser Thr Cys Arg Met Ser Gly Lys Gly625 630 635 640cca gga tat gga gct tgt gat acc aaa gga aaa tta ttt gaa tgt agc 1968Pro Gly Tyr Gly Ala Cys Asp Thr Lys Gly Lys Leu Phe Glu Cys Ser 645 650 655aat gtt tat gtt gca gat gct tcc act ttg cct act gca tca ggg gct 2016Asn Val Tyr Val Ala Asp Ala Ser Thr Leu Pro Thr Ala Ser Gly Ala 660 665 670aat cca atg gtt agt acc atg tca ttt gca agg cac gtg tcc tta gga 2064Asn Pro Met Val Ser Thr Met Ser Phe Ala Arg His Val Ser Leu Gly 675 680 685att gtt aaa gaa ttg caa caa agt aaa ctt tag 2097Ile Val Lys Glu Leu Gln Gln Ser Lys Leu 690 69556698PRTCandida cloacae 56Met Asn Pro Val Val Glu Asp Ser His Leu Asp Val Phe Cys Leu Leu1 5 10 15Ala Asp Ala Val Val His Glu Ile Pro Pro Ser Glu Ile Val Glu Tyr 20 25 30Leu His Pro Asp Phe Pro Lys Asp Lys Val Glu Glu Tyr Leu Ala Glu 35 40 45Phe Ser His Pro Ser Ala Ile Pro Glu Phe Arg Glu Val Ala Lys Arg 50 55 60Ile Ile Asn Lys Gly Thr Val Leu Ser Ile Lys Leu Phe Leu Leu Leu65 70 75 80Ala Thr Ala Leu Asp Ser Arg Ile Leu Ala Pro Ala Leu Thr Asn Ser 85 90 95Thr Thr Leu Ile Arg Asp Met Asp Leu Ser Gln Arg Glu Glu Leu Leu 100 105 110Arg Ser Trp Arg Asp Ser Pro Phe Thr Thr Lys Arg Lys Leu Phe Arg 115 120 125Val Tyr Asn Ser Phe Thr Leu Asn Ala Phe Ser Lys Thr Ala Thr Asp 130 135 140Leu His Phe Lys Ala Leu Gly Tyr Pro Gly Arg Glu Leu Arg Thr Gln145 150 155 160Ile Gln Asp Tyr Glu Val Asp Pro Phe Arg Tyr Thr Phe Leu Glu Lys 165 170 175Pro Gln Gln Asp Gly Gln Glu Leu His Phe Pro Asp Ile Asp Val Leu 180 185 190Ile Ile Gly Ser Gly Ser Gly Ala Gly Val Val Ala Gln Thr Leu Ser 195 200 205Glu Asn Gly Leu Lys Ser Leu Val Leu Glu Lys Gly Lys Tyr Phe Ser 210 215 220Asn Asp Glu Leu Thr Met Asn Asp Leu Glu Gly Ser Glu Ala Leu Phe225 230 235 240Glu Asn Gly Gly Ala Leu Ser Ser Thr Asn Gln Gln Ile Phe Ile Ile 245 250 255Ala Gly Ser Thr Phe Gly Gly Gly Ser Thr Val Asn Trp Ser Ala Cys 260 265 270Leu Lys Thr Pro Phe Lys Val Arg Lys Glu Trp Tyr Asp Asn Phe Gly 275 280 285Leu Asp Phe Val Ala Thr Gln Tyr Tyr Glu Asp Cys Met Asp Tyr Val 290 295 300Trp Lys Lys Met Gly Ala Ser Asn Glu Asn Ile Asp His Ser Gly Ala305 310 315 320Asn Ser Val Ile Leu Glu Gly Ser Lys Lys Leu Gly Tyr Pro His Arg 325 330 335Ala Val Glu Gln Asn Asn Gly Gly Lys Ile His Asp Cys Gly Met Cys 340 345 350His Leu Gly Cys Arg Phe Gly Ile Lys Gln Gly Ser Val Asn Cys Trp 355 360 365Phe Arg Gly Pro Ser Glu Asn Gly Ser Lys Phe Met Gln Gln Val Leu 370 375 380Val Asp Lys Ile Leu Gln Arg Asp Gly Lys Ala Val Gly Val Leu Cys385 390 395 400Arg Asp Val Val Thr Gly Val Lys Phe Lys Ile Thr Gly Pro Lys Lys 405 410 415Ile Val Val Phe Trp Trp Phe Phe Ala Asn Ser Gly Phe Val Thr Lys 420 425 430Ser Gly Phe Lys Asn Lys His Ile Gly Ala Asn Leu Lys Leu His Pro 435 440 445Val Ser Leu Thr Leu Gly Asp Phe Gly Asn Asn Val Asp Phe Glu Ala 450 455 460Tyr Arg Lys Pro Ile Met Thr Ser Ile Cys Asn Lys Val Glu Asp Leu465 470 475 480Asp Gly Lys Ala His Gly Thr Arg Ile Glu Ala Met Leu Asn Ala Pro 485 490

495Tyr Gly Val Ala Pro Phe Phe Pro Trp Lys Ser Gly Ala Glu Ser Arg 500 505 510Lys Asp Leu Leu Arg Tyr Lys Gln Thr Val Pro Ile Leu Leu Leu Ser 515 520 525Arg Asp Thr Thr Ser Gly Ser Val Thr Tyr Asp Lys Gln Lys Pro Asp 530 535 540Ala Leu Val Ile Asp Tyr Leu Leu Asn Lys Phe Asp Arg Asn Ser Ile545 550 555 560Leu Gln Gly Phe Leu Ile Ala Ser Asp Leu Leu Tyr Ile Glu Gly Ala 565 570 575Ser Arg Asp His Val Thr Tyr Lys Leu Gly Tyr Gln Trp Phe Lys Ser 580 585 590Ser Lys Pro Lys His Ala Arg Ser Ile Glu Asp Glu Asp Tyr Val Asn 595 600 605Trp Arg Ala Lys Val Ala Lys Ile Pro Phe Asp Ser Tyr Gly Ser Pro 610 615 620Tyr Gly Ser Ala His Gln Met Ser Thr Cys Arg Met Ser Gly Lys Gly625 630 635 640Pro Gly Tyr Gly Ala Cys Asp Thr Lys Gly Lys Leu Phe Glu Cys Ser 645 650 655Asn Val Tyr Val Ala Asp Ala Ser Thr Leu Pro Thr Ala Ser Gly Ala 660 665 670Asn Pro Met Val Ser Thr Met Ser Phe Ala Arg His Val Ser Leu Gly 675 680 685Ile Val Lys Glu Leu Gln Gln Ser Lys Leu 690 69557948DNACandida tropicalisCDS(1)..(948) 57cca ttg caa tac acc gat atc cca gtt cca gtc cct aag cca aac gaa 48Pro Leu Gln Tyr Thr Asp Ile Pro Val Pro Val Pro Lys Pro Asn Glu1 5 10 15ttg ctc gtc cac gtc aaa tac tcc ggt gtt tgt cac tca gat ata cac 96Leu Leu Val His Val Lys Tyr Ser Gly Val Cys His Ser Asp Ile His 20 25 30gtc tgg aag ggt gac tgg ttc cca gca tcg aaa ttg ccc gtt gtt ggt 144Val Trp Lys Gly Asp Trp Phe Pro Ala Ser Lys Leu Pro Val Val Gly 35 40 45ggt cac gaa ggt gcc ggt gtt gtc gtt gcc att ggt gaa aac gtc caa 192Gly His Glu Gly Ala Gly Val Val Val Ala Ile Gly Glu Asn Val Gln 50 55 60ggc tgg aaa gta ggt gac ttg gca ggt ata aag atg ttg aat ggt tcc 240Gly Trp Lys Val Gly Asp Leu Ala Gly Ile Lys Met Leu Asn Gly Ser65 70 75 80tgt atg aac tgt gaa tac tgt caa caa ggt gct gaa cca aac tgt ccc 288Cys Met Asn Cys Glu Tyr Cys Gln Gln Gly Ala Glu Pro Asn Cys Pro 85 90 95cac gct gat gtc tcg ggt tac tcc cac gac ggt act ttc caa cag tac 336His Ala Asp Val Ser Gly Tyr Ser His Asp Gly Thr Phe Gln Gln Tyr 100 105 110gct acc gcc gat gct gtt caa gct gct aaa ttc cca gct ggt tct gat 384Ala Thr Ala Asp Ala Val Gln Ala Ala Lys Phe Pro Ala Gly Ser Asp 115 120 125tta gct agc atc gca cct ata tcc tgc gcc ggt gtt act gtt tac aaa 432Leu Ala Ser Ile Ala Pro Ile Ser Cys Ala Gly Val Thr Val Tyr Lys 130 135 140gca ttg aaa act gca ggc ttg cag cca ggt caa tgg gtt gcc atc tct 480Ala Leu Lys Thr Ala Gly Leu Gln Pro Gly Gln Trp Val Ala Ile Ser145 150 155 160ggt gca gct ggt ggt ttg ggt tct ttg gct gtg caa tac gcc aag gcc 528Gly Ala Ala Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Lys Ala 165 170 175atg ggt ttg aga gtc gtg gcc att gac ggt ggt gac gaa aga gga gtg 576Met Gly Leu Arg Val Val Ala Ile Asp Gly Gly Asp Glu Arg Gly Val 180 185 190ttt gtc aaa tcg ttg ggt gct gaa gtt ttc gtt gat ttc acc aaa gag 624Phe Val Lys Ser Leu Gly Ala Glu Val Phe Val Asp Phe Thr Lys Glu 195 200 205gcc aat gtc tct gag gct atc atc aag gct acc gac ggt ggt gcc cat 672Ala Asn Val Ser Glu Ala Ile Ile Lys Ala Thr Asp Gly Gly Ala His 210 215 220ggc gtc atc aac gtt tcc att tct gaa aaa gcc atc aac cag tct gtt 720Gly Val Ile Asn Val Ser Ile Ser Glu Lys Ala Ile Asn Gln Ser Val225 230 235 240gaa tat gtt aga act ttg gga act gtt gtc ttg gtt ggt ttg cca gct 768Glu Tyr Val Arg Thr Leu Gly Thr Val Val Leu Val Gly Leu Pro Ala 245 250 255ggt gca aag ctc gaa gct cct atc ttc aat gcc gtt gcc aaa tcc atc 816Gly Ala Lys Leu Glu Ala Pro Ile Phe Asn Ala Val Ala Lys Ser Ile 260 265 270caa atc aaa ggt tct tac gtg gga aac aga aga gac act gct gag gct 864Gln Ile Lys Gly Ser Tyr Val Gly Asn Arg Arg Asp Thr Ala Glu Ala 275 280 285gtt gat ttc ttc gct aga ggt ttg gtc aaa tgt cca att aag gtt gtt 912Val Asp Phe Phe Ala Arg Gly Leu Val Lys Cys Pro Ile Lys Val Val 290 295 300ggg ttg agt gaa ttg cca gag att ttc aaa ttg ttg 948Gly Leu Ser Glu Leu Pro Glu Ile Phe Lys Leu Leu305 310 31558316PRTCandida tropicalis 58Pro Leu Gln Tyr Thr Asp Ile Pro Val Pro Val Pro Lys Pro Asn Glu1 5 10 15Leu Leu Val His Val Lys Tyr Ser Gly Val Cys His Ser Asp Ile His 20 25 30Val Trp Lys Gly Asp Trp Phe Pro Ala Ser Lys Leu Pro Val Val Gly 35 40 45Gly His Glu Gly Ala Gly Val Val Val Ala Ile Gly Glu Asn Val Gln 50 55 60Gly Trp Lys Val Gly Asp Leu Ala Gly Ile Lys Met Leu Asn Gly Ser65 70 75 80Cys Met Asn Cys Glu Tyr Cys Gln Gln Gly Ala Glu Pro Asn Cys Pro 85 90 95His Ala Asp Val Ser Gly Tyr Ser His Asp Gly Thr Phe Gln Gln Tyr 100 105 110Ala Thr Ala Asp Ala Val Gln Ala Ala Lys Phe Pro Ala Gly Ser Asp 115 120 125Leu Ala Ser Ile Ala Pro Ile Ser Cys Ala Gly Val Thr Val Tyr Lys 130 135 140Ala Leu Lys Thr Ala Gly Leu Gln Pro Gly Gln Trp Val Ala Ile Ser145 150 155 160Gly Ala Ala Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Lys Ala 165 170 175Met Gly Leu Arg Val Val Ala Ile Asp Gly Gly Asp Glu Arg Gly Val 180 185 190Phe Val Lys Ser Leu Gly Ala Glu Val Phe Val Asp Phe Thr Lys Glu 195 200 205Ala Asn Val Ser Glu Ala Ile Ile Lys Ala Thr Asp Gly Gly Ala His 210 215 220Gly Val Ile Asn Val Ser Ile Ser Glu Lys Ala Ile Asn Gln Ser Val225 230 235 240Glu Tyr Val Arg Thr Leu Gly Thr Val Val Leu Val Gly Leu Pro Ala 245 250 255Gly Ala Lys Leu Glu Ala Pro Ile Phe Asn Ala Val Ala Lys Ser Ile 260 265 270Gln Ile Lys Gly Ser Tyr Val Gly Asn Arg Arg Asp Thr Ala Glu Ala 275 280 285Val Asp Phe Phe Ala Arg Gly Leu Val Lys Cys Pro Ile Lys Val Val 290 295 300Gly Leu Ser Glu Leu Pro Glu Ile Phe Lys Leu Leu305 310 31559948DNACandida tropicalisCDS(1)..(948) 59aaa tta gaa tac aag gac atc cca gtt cca aag cca aag cca aac gaa 48Lys Leu Glu Tyr Lys Asp Ile Pro Val Pro Lys Pro Lys Pro Asn Glu1 5 10 15ttg ctc atc aac gtc aag tac tcc ggt gtc tgc cac act gat tta cac 96Leu Leu Ile Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu His 20 25 30gcc tgg aag ggt gac tgg cca ttg gac acc aag ttg cca ttg gtg ggt 144Ala Trp Lys Gly Asp Trp Pro Leu Asp Thr Lys Leu Pro Leu Val Gly 35 40 45ggt cac gaa ggt gct ggt gtt gtt gtt gcc att ggt gac aat gtc aag 192Gly His Glu Gly Ala Gly Val Val Val Ala Ile Gly Asp Asn Val Lys 50 55 60gga tgg aag gtc ggt gat ttg gcc ggt gtc aag tgg ttg aac ggt tcc 240Gly Trp Lys Val Gly Asp Leu Ala Gly Val Lys Trp Leu Asn Gly Ser65 70 75 80tgt atg aac tgt gag tac tgt caa cag ggt gcc gaa cca aac tgt cca 288Cys Met Asn Cys Glu Tyr Cys Gln Gln Gly Ala Glu Pro Asn Cys Pro 85 90 95cag gct gac ttg tct ggt tac acc cac gac ggt tct ttc cag caa tac 336Gln Ala Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Gln Tyr 100 105 110gcc act gca gat gcc gtg caa gcc gct aga att cca gct ggt act gat 384Ala Thr Ala Asp Ala Val Gln Ala Ala Arg Ile Pro Ala Gly Thr Asp 115 120 125tta gcc aac gtt gcc ccc atc ttg tgt gct ggt gtc act gtt tac aag 432Leu Ala Asn Val Ala Pro Ile Leu Cys Ala Gly Val Thr Val Tyr Lys 130 135 140gcc ttg aag acc gcc gac ttg cag cca ggt caa tgg gtc gcc att tcc 480Ala Leu Lys Thr Ala Asp Leu Gln Pro Gly Gln Trp Val Ala Ile Ser145 150 155 160ggt gcc gct ggt ggt ttg ggt tct ttg gcc gtt caa tac gcc aag gcc 528Gly Ala Ala Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Lys Ala 165 170 175atg ggc tac aga gtt gtc gcc atc gat ggt ggt gcc gac aag ggt gag 576Met Gly Tyr Arg Val Val Ala Ile Asp Gly Gly Ala Asp Lys Gly Glu 180 185 190ttc gtc aag tct ttg ggc gct gag gtc ttt gtt gat ttc ctc aag gaa 624Phe Val Lys Ser Leu Gly Ala Glu Val Phe Val Asp Phe Leu Lys Glu 195 200 205aag gac att gtt ggt gct gtc aag aag gca acc gat ggt ggc cca cac 672Lys Asp Ile Val Gly Ala Val Lys Lys Ala Thr Asp Gly Gly Pro His 210 215 220ggt gcc gtt aac gtt tcc atc tcc gaa aag gcc atc aac caa tct gtc 720Gly Ala Val Asn Val Ser Ile Ser Glu Lys Ala Ile Asn Gln Ser Val225 230 235 240gac tac gtt aga acc ttg ggt aag gtt gtc ttg gtc ggt ttg cca gct 768Asp Tyr Val Arg Thr Leu Gly Lys Val Val Leu Val Gly Leu Pro Ala 245 250 255ggc tcc aag gtt tct gct cca gtc ttt gac tcc gtc gtc aag tcc atc 816Gly Ser Lys Val Ser Ala Pro Val Phe Asp Ser Val Val Lys Ser Ile 260 265 270caa atc aag ggt tcc tat gtc ggt aac aga aag gac act gcc gaa gct 864Gln Ile Lys Gly Ser Tyr Val Gly Asn Arg Lys Asp Thr Ala Glu Ala 275 280 285gtt gac ttt ttc tcc aga ggc ttg atc aag tgt cca atc aag gtt gtc 912Val Asp Phe Phe Ser Arg Gly Leu Ile Lys Cys Pro Ile Lys Val Val 290 295 300ggt ttg agt gaa ttg cca gaa gtc tac aag ttg atg 948Gly Leu Ser Glu Leu Pro Glu Val Tyr Lys Leu Met305 310 31560316PRTCandida tropicalis 60Lys Leu Glu Tyr Lys Asp Ile Pro Val Pro Lys Pro Lys Pro Asn Glu1 5 10 15Leu Leu Ile Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu His 20 25 30Ala Trp Lys Gly Asp Trp Pro Leu Asp Thr Lys Leu Pro Leu Val Gly 35 40 45Gly His Glu Gly Ala Gly Val Val Val Ala Ile Gly Asp Asn Val Lys 50 55 60Gly Trp Lys Val Gly Asp Leu Ala Gly Val Lys Trp Leu Asn Gly Ser65 70 75 80Cys Met Asn Cys Glu Tyr Cys Gln Gln Gly Ala Glu Pro Asn Cys Pro 85 90 95Gln Ala Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Gln Tyr 100 105 110Ala Thr Ala Asp Ala Val Gln Ala Ala Arg Ile Pro Ala Gly Thr Asp 115 120 125Leu Ala Asn Val Ala Pro Ile Leu Cys Ala Gly Val Thr Val Tyr Lys 130 135 140Ala Leu Lys Thr Ala Asp Leu Gln Pro Gly Gln Trp Val Ala Ile Ser145 150 155 160Gly Ala Ala Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Lys Ala 165 170 175Met Gly Tyr Arg Val Val Ala Ile Asp Gly Gly Ala Asp Lys Gly Glu 180 185 190Phe Val Lys Ser Leu Gly Ala Glu Val Phe Val Asp Phe Leu Lys Glu 195 200 205Lys Asp Ile Val Gly Ala Val Lys Lys Ala Thr Asp Gly Gly Pro His 210 215 220Gly Ala Val Asn Val Ser Ile Ser Glu Lys Ala Ile Asn Gln Ser Val225 230 235 240Asp Tyr Val Arg Thr Leu Gly Lys Val Val Leu Val Gly Leu Pro Ala 245 250 255Gly Ser Lys Val Ser Ala Pro Val Phe Asp Ser Val Val Lys Ser Ile 260 265 270Gln Ile Lys Gly Ser Tyr Val Gly Asn Arg Lys Asp Thr Ala Glu Ala 275 280 285Val Asp Phe Phe Ser Arg Gly Leu Ile Lys Cys Pro Ile Lys Val Val 290 295 300Gly Leu Ser Glu Leu Pro Glu Val Tyr Lys Leu Met305 310 31561456DNACandida tropicalisCDS(1)..(456) 61aac tgt gag ttt tgc caa cag ggc gct gaa cct aat tgt cca aga gcc 48Asn Cys Glu Phe Cys Gln Gln Gly Ala Glu Pro Asn Cys Pro Arg Ala1 5 10 15gac atg tct gga tat acc cac gat ggg act ttc caa caa tat gct acc 96Asp Met Ser Gly Tyr Thr His Asp Gly Thr Phe Gln Gln Tyr Ala Thr 20 25 30gcc gat gcc gtc caa gct gcc aag atc cca gaa ggc gca gac atg gct 144Ala Asp Ala Val Gln Ala Ala Lys Ile Pro Glu Gly Ala Asp Met Ala 35 40 45agt atc gcc ccg atc ttg tgt gct ggt gtg acc gtg tac aag gct ttg 192Ser Ile Ala Pro Ile Leu Cys Ala Gly Val Thr Val Tyr Lys Ala Leu 50 55 60aag aac gcc gac ttg ttg gct ggc caa tgg gtg gct atc tct ggt gct 240Lys Asn Ala Asp Leu Leu Ala Gly Gln Trp Val Ala Ile Ser Gly Ala65 70 75 80ggt ggt ggt ttg ggc tcc ttg ggt gtg cag tac gct aaa gcc atg ggt 288Gly Gly Gly Leu Gly Ser Leu Gly Val Gln Tyr Ala Lys Ala Met Gly 85 90 95tac aga gtg tta gcc atc gat ggt ggt gat gag aga gga gag ttt gtc 336Tyr Arg Val Leu Ala Ile Asp Gly Gly Asp Glu Arg Gly Glu Phe Val 100 105 110aag tca ttg ggc gcc gaa gtg tac att gac ttc ctt aag gaa cag gac 384Lys Ser Leu Gly Ala Glu Val Tyr Ile Asp Phe Leu Lys Glu Gln Asp 115 120 125att gtt agt gcc att aga aag gca act ggt ggt ggc cca cac ggt gtt 432Ile Val Ser Ala Ile Arg Lys Ala Thr Gly Gly Gly Pro His Gly Val 130 135 140att aac gtc tcg gtg tcc gaa aag 456Ile Asn Val Ser Val Ser Glu Lys145 15062152PRTCandida tropicalis 62Asn Cys Glu Phe Cys Gln Gln Gly Ala Glu Pro Asn Cys Pro Arg Ala1 5 10 15Asp Met Ser Gly Tyr Thr His Asp Gly Thr Phe Gln Gln Tyr Ala Thr 20 25 30Ala Asp Ala Val Gln Ala Ala Lys Ile Pro Glu Gly Ala Asp Met Ala 35 40 45Ser Ile Ala Pro Ile Leu Cys Ala Gly Val Thr Val Tyr Lys Ala Leu 50 55 60Lys Asn Ala Asp Leu Leu Ala Gly Gln Trp Val Ala Ile Ser Gly Ala65 70 75 80Gly Gly Gly Leu Gly Ser Leu Gly Val Gln Tyr Ala Lys Ala Met Gly 85 90 95Tyr Arg Val Leu Ala Ile Asp Gly Gly Asp Glu Arg Gly Glu Phe Val 100 105 110Lys Ser Leu Gly Ala Glu Val Tyr Ile Asp Phe Leu Lys Glu Gln Asp 115 120 125Ile Val Ser Ala Ile Arg Lys Ala Thr Gly Gly Gly Pro His Gly Val 130 135 140Ile Asn Val Ser Val Ser Glu Lys145 15063948DNACandida tropicalisCDS(1)..(948) 63aag tta gaa tac aaa gac gtg ccg gtc cct gtc cct aaa ccc aac gaa 48Lys Leu Glu Tyr Lys Asp Val Pro Val Pro Val Pro Lys Pro Asn Glu1 5 10 15ttg ctt gtc aac gtc aag tac tcg ggt gtg tgt cat tct gac ttg cat 96Leu Leu Val Asn Val Lys Tyr Ser Gly Val Cys His Ser Asp Leu His 20 25 30gtc tgg aaa ggc gac tgg ccc att cct gcc aag ttg ccc ttg gtg gga 144Val Trp Lys Gly Asp Trp Pro Ile Pro Ala Lys Leu Pro Leu Val Gly 35 40 45ggt cac gaa ggt gct ggt gtc gtt gtc ggc atg ggt gac aac gtc aag 192Gly His Glu Gly Ala Gly Val Val Val Gly Met Gly Asp Asn Val Lys 50 55 60ggc tgg aag gtg ggg gac ttg gct ggt atc aag tgg ttg aat ggt tcg 240Gly Trp Lys Val Gly Asp Leu Ala Gly Ile Lys Trp Leu Asn Gly Ser65 70 75 80tgt atg aac tgt gag ttt tgc caa cag ggc gca gaa cct aac tgt tca 288Cys Met Asn Cys Glu Phe Cys Gln Gln Gly Ala Glu Pro Asn Cys Ser 85 90 95aga gcc gac atg tct ggg tat acc cac gat gga act ttc caa caa tac 336Arg Ala Asp Met Ser Gly Tyr Thr His Asp Gly Thr Phe Gln Gln Tyr 100 105 110gcc act gct gat gct gtc caa gct gcc aag atc cca gaa ggc gcc gac 384Ala

Thr Ala Asp Ala Val Gln Ala Ala Lys Ile Pro Glu Gly Ala Asp 115 120 125atg gct agt atc gcc ccg atc ttg tgc gct ggt gtg acc gtg tac aag 432Met Ala Ser Ile Ala Pro Ile Leu Cys Ala Gly Val Thr Val Tyr Lys 130 135 140gct ttg aag aac gcc gac ttg ttg gct ggc caa tgg gtg gct atc tct 480Ala Leu Lys Asn Ala Asp Leu Leu Ala Gly Gln Trp Val Ala Ile Ser145 150 155 160ggt gct ggt ggt ggt ttg ggc tcc ttg ggt gtg cag tac gct aaa gcc 528Gly Ala Gly Gly Gly Leu Gly Ser Leu Gly Val Gln Tyr Ala Lys Ala 165 170 175atg ggt tac aga gtg ttg gct atc gac ggt ggt gac gag aga gga gag 576Met Gly Tyr Arg Val Leu Ala Ile Asp Gly Gly Asp Glu Arg Gly Glu 180 185 190ttt gtc aag tcc ttg ggc gcc gaa gtg tac att gac ttc ctt aag gaa 624Phe Val Lys Ser Leu Gly Ala Glu Val Tyr Ile Asp Phe Leu Lys Glu 195 200 205cag gac atc gtt agt gct atc aga aag gca act ggt ggt ggt cca cac 672Gln Asp Ile Val Ser Ala Ile Arg Lys Ala Thr Gly Gly Gly Pro His 210 215 220ggt gtt att aac gtg tca gtg tcc gaa aag gca atc aac cag tcg gtg 720Gly Val Ile Asn Val Ser Val Ser Glu Lys Ala Ile Asn Gln Ser Val225 230 235 240gag tac gtc aga act ttg ggg aaa gtg gtt tta gtt agc ttg ccg gca 768Glu Tyr Val Arg Thr Leu Gly Lys Val Val Leu Val Ser Leu Pro Ala 245 250 255ggt ggt aaa ctc act gct cct ctt ttc gag tct gtt gct aga tca atc 816Gly Gly Lys Leu Thr Ala Pro Leu Phe Glu Ser Val Ala Arg Ser Ile 260 265 270cag att aga act acg tgt gtt ggc aac aga aag gat act act gaa gct 864Gln Ile Arg Thr Thr Cys Val Gly Asn Arg Lys Asp Thr Thr Glu Ala 275 280 285att gat ttc ttt gtt aga ggg ttg atc gat tgc cca att aaa gtc gct 912Ile Asp Phe Phe Val Arg Gly Leu Ile Asp Cys Pro Ile Lys Val Ala 290 295 300ggt tta agt gaa gtg cca gag att ttt gac ttg atg 948Gly Leu Ser Glu Val Pro Glu Ile Phe Asp Leu Met305 310 31564316PRTCandida tropicalis 64Lys Leu Glu Tyr Lys Asp Val Pro Val Pro Val Pro Lys Pro Asn Glu1 5 10 15Leu Leu Val Asn Val Lys Tyr Ser Gly Val Cys His Ser Asp Leu His 20 25 30Val Trp Lys Gly Asp Trp Pro Ile Pro Ala Lys Leu Pro Leu Val Gly 35 40 45Gly His Glu Gly Ala Gly Val Val Val Gly Met Gly Asp Asn Val Lys 50 55 60Gly Trp Lys Val Gly Asp Leu Ala Gly Ile Lys Trp Leu Asn Gly Ser65 70 75 80Cys Met Asn Cys Glu Phe Cys Gln Gln Gly Ala Glu Pro Asn Cys Ser 85 90 95Arg Ala Asp Met Ser Gly Tyr Thr His Asp Gly Thr Phe Gln Gln Tyr 100 105 110Ala Thr Ala Asp Ala Val Gln Ala Ala Lys Ile Pro Glu Gly Ala Asp 115 120 125Met Ala Ser Ile Ala Pro Ile Leu Cys Ala Gly Val Thr Val Tyr Lys 130 135 140Ala Leu Lys Asn Ala Asp Leu Leu Ala Gly Gln Trp Val Ala Ile Ser145 150 155 160Gly Ala Gly Gly Gly Leu Gly Ser Leu Gly Val Gln Tyr Ala Lys Ala 165 170 175Met Gly Tyr Arg Val Leu Ala Ile Asp Gly Gly Asp Glu Arg Gly Glu 180 185 190Phe Val Lys Ser Leu Gly Ala Glu Val Tyr Ile Asp Phe Leu Lys Glu 195 200 205Gln Asp Ile Val Ser Ala Ile Arg Lys Ala Thr Gly Gly Gly Pro His 210 215 220Gly Val Ile Asn Val Ser Val Ser Glu Lys Ala Ile Asn Gln Ser Val225 230 235 240Glu Tyr Val Arg Thr Leu Gly Lys Val Val Leu Val Ser Leu Pro Ala 245 250 255Gly Gly Lys Leu Thr Ala Pro Leu Phe Glu Ser Val Ala Arg Ser Ile 260 265 270Gln Ile Arg Thr Thr Cys Val Gly Asn Arg Lys Asp Thr Thr Glu Ala 275 280 285Ile Asp Phe Phe Val Arg Gly Leu Ile Asp Cys Pro Ile Lys Val Ala 290 295 300Gly Leu Ser Glu Val Pro Glu Ile Phe Asp Leu Met305 310 31565948DNACandida tropicalisCDS(1)..(948) 65gaa tta gaa tac aaa gat atc cca gtg cca acc cca aag gcc aac gaa 48Glu Leu Glu Tyr Lys Asp Ile Pro Val Pro Thr Pro Lys Ala Asn Glu1 5 10 15ttg ctc atc aac gtc aaa tac tcg ggt gtc tgc cac act gat ttg cac 96Leu Leu Ile Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu His 20 25 30gcc tgg aag ggt gac tgg cca ttg gcc acc aag ttg cca ttg gtt ggt 144Ala Trp Lys Gly Asp Trp Pro Leu Ala Thr Lys Leu Pro Leu Val Gly 35 40 45ggt cac gaa ggt gct ggt gtc gtt gtc ggc atg ggt gaa aac gtc aag 192Gly His Glu Gly Ala Gly Val Val Val Gly Met Gly Glu Asn Val Lys 50 55 60ggc tgg aag att ggt gac ttc gcc ggt atc aaa tgg ttg aac ggt tcc 240Gly Trp Lys Ile Gly Asp Phe Ala Gly Ile Lys Trp Leu Asn Gly Ser65 70 75 80tgt atg tcc tgt gag ttc tgt caa caa ggt gct gaa cca aac tgt ggt 288Cys Met Ser Cys Glu Phe Cys Gln Gln Gly Ala Glu Pro Asn Cys Gly 85 90 95gag gcc gac ttg tct ggt tac acc cac gat ggt tct ttc gaa caa tac 336Glu Ala Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe Glu Gln Tyr 100 105 110gcc act gct gat gct gtt caa gcc gcc aga atc cca gct ggt act gat 384Ala Thr Ala Asp Ala Val Gln Ala Ala Arg Ile Pro Ala Gly Thr Asp 115 120 125ttg gcc gaa gtt gcc cca atc ttg tgt gcg ggt gtc acc gtc tac aaa 432Leu Ala Glu Val Ala Pro Ile Leu Cys Ala Gly Val Thr Val Tyr Lys 130 135 140gcc ttg aag act gcc gac ttg gcc gct ggt caa tgg gtc gct atc tcc 480Ala Leu Lys Thr Ala Asp Leu Ala Ala Gly Gln Trp Val Ala Ile Ser145 150 155 160ggt gct ggt ggt ggt ttg ggt tcc ttg gct gtc caa tac gcc gtc gcc 528Gly Ala Gly Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Val Ala 165 170 175atg ggc ttg aga gtc gtt gcc att gac ggt ggt gac gaa aag ggt gcc 576Met Gly Leu Arg Val Val Ala Ile Asp Gly Gly Asp Glu Lys Gly Ala 180 185 190ttt gtc aag tcc ttg ggt gct gaa gcc tac att gat ttc ctc aag gaa 624Phe Val Lys Ser Leu Gly Ala Glu Ala Tyr Ile Asp Phe Leu Lys Glu 195 200 205aag gac att gtc tct gct gtc aag aag gcc acc gat gga ggt cca cac 672Lys Asp Ile Val Ser Ala Val Lys Lys Ala Thr Asp Gly Gly Pro His 210 215 220ggt gct atc aat gtt tcc gtt tcc gaa aaa gcc att gac caa tcc gtc 720Gly Ala Ile Asn Val Ser Val Ser Glu Lys Ala Ile Asp Gln Ser Val225 230 235 240gag tac gtt aga cca ttg ggt aag gtt gtt ttg gtt ggt ttg cca gct 768Glu Tyr Val Arg Pro Leu Gly Lys Val Val Leu Val Gly Leu Pro Ala 245 250 255ggc tcc aag gtc act gct ggt gtt ttc gaa gcc gtt gtc aag tcc att 816Gly Ser Lys Val Thr Ala Gly Val Phe Glu Ala Val Val Lys Ser Ile 260 265 270gaa atc aag ggt tcc tat gtc ggt aac aga aag gat acc gcc gaa gcc 864Glu Ile Lys Gly Ser Tyr Val Gly Asn Arg Lys Asp Thr Ala Glu Ala 275 280 285gtt gac ttt ttc tcc aga ggc ttg atc aag tgt cca atc aag att gtt 912Val Asp Phe Phe Ser Arg Gly Leu Ile Lys Cys Pro Ile Lys Ile Val 290 295 300ggc ttg agt gaa ttg cca cag gtc ttc aag ttg atg 948Gly Leu Ser Glu Leu Pro Gln Val Phe Lys Leu Met305 310 31566316PRTCandida tropicalis 66Glu Leu Glu Tyr Lys Asp Ile Pro Val Pro Thr Pro Lys Ala Asn Glu1 5 10 15Leu Leu Ile Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu His 20 25 30Ala Trp Lys Gly Asp Trp Pro Leu Ala Thr Lys Leu Pro Leu Val Gly 35 40 45Gly His Glu Gly Ala Gly Val Val Val Gly Met Gly Glu Asn Val Lys 50 55 60Gly Trp Lys Ile Gly Asp Phe Ala Gly Ile Lys Trp Leu Asn Gly Ser65 70 75 80Cys Met Ser Cys Glu Phe Cys Gln Gln Gly Ala Glu Pro Asn Cys Gly 85 90 95Glu Ala Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe Glu Gln Tyr 100 105 110Ala Thr Ala Asp Ala Val Gln Ala Ala Arg Ile Pro Ala Gly Thr Asp 115 120 125Leu Ala Glu Val Ala Pro Ile Leu Cys Ala Gly Val Thr Val Tyr Lys 130 135 140Ala Leu Lys Thr Ala Asp Leu Ala Ala Gly Gln Trp Val Ala Ile Ser145 150 155 160Gly Ala Gly Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Val Ala 165 170 175Met Gly Leu Arg Val Val Ala Ile Asp Gly Gly Asp Glu Lys Gly Ala 180 185 190Phe Val Lys Ser Leu Gly Ala Glu Ala Tyr Ile Asp Phe Leu Lys Glu 195 200 205Lys Asp Ile Val Ser Ala Val Lys Lys Ala Thr Asp Gly Gly Pro His 210 215 220Gly Ala Ile Asn Val Ser Val Ser Glu Lys Ala Ile Asp Gln Ser Val225 230 235 240Glu Tyr Val Arg Pro Leu Gly Lys Val Val Leu Val Gly Leu Pro Ala 245 250 255Gly Ser Lys Val Thr Ala Gly Val Phe Glu Ala Val Val Lys Ser Ile 260 265 270Glu Ile Lys Gly Ser Tyr Val Gly Asn Arg Lys Asp Thr Ala Glu Ala 275 280 285Val Asp Phe Phe Ser Arg Gly Leu Ile Lys Cys Pro Ile Lys Ile Val 290 295 300Gly Leu Ser Glu Leu Pro Gln Val Phe Lys Leu Met305 310 315671992DNACandida tropicalisCDS(1)..(1992) 67ctt atg tta tta tgt gac ggg atc atc cac gaa acc acc gtc gac caa 48Leu Met Leu Leu Cys Asp Gly Ile Ile His Glu Thr Thr Val Asp Gln1 5 10 15atc aaa gac gtt att gct cct gac ttc cct gct gac aag tac gaa gag 96Ile Lys Asp Val Ile Ala Pro Asp Phe Pro Ala Asp Lys Tyr Glu Glu 20 25 30tac gtc agg aca ttc acc aaa ccc tcc gaa acc cca ggg ttc agg gaa 144Tyr Val Arg Thr Phe Thr Lys Pro Ser Glu Thr Pro Gly Phe Arg Glu 35 40 45acc gtc tac aac aca gtc aac gca aac acc acg gac gca atc cac cag 192Thr Val Tyr Asn Thr Val Asn Ala Asn Thr Thr Asp Ala Ile His Gln 50 55 60ttc att atc ttg acc aat gtt ttg gca tcc agg gtc ttg gct cca gct 240Phe Ile Ile Leu Thr Asn Val Leu Ala Ser Arg Val Leu Ala Pro Ala65 70 75 80ttg acc aac tcg ttg acg cct atc aag gac atg agc ttg gaa gac cgt 288Leu Thr Asn Ser Leu Thr Pro Ile Lys Asp Met Ser Leu Glu Asp Arg 85 90 95gaa aaa ttg ttg gcc tcg tgg cgc gac tcc cca atc gct gcc aaa agg 336Glu Lys Leu Leu Ala Ser Trp Arg Asp Ser Pro Ile Ala Ala Lys Arg 100 105 110aaa ttg ttc agg ttg gtt tcc acg ctt acc ttg gtt act ttc acg aga 384Lys Leu Phe Arg Leu Val Ser Thr Leu Thr Leu Val Thr Phe Thr Arg 115 120 125ttg gcc aat gag ttg cat ttg aaa gcc att cac tat cca gga aga gaa 432Leu Ala Asn Glu Leu His Leu Lys Ala Ile His Tyr Pro Gly Arg Glu 130 135 140gac cgt gaa aag gct tat gaa acc cag gag att gac cct ttc aag tac 480Asp Arg Glu Lys Ala Tyr Glu Thr Gln Glu Ile Asp Pro Phe Lys Tyr145 150 155 160cag ttt atg gaa aag cca aag ttt gac ggc gct gag ttg tac ttg cca 528Gln Phe Met Glu Lys Pro Lys Phe Asp Gly Ala Glu Leu Tyr Leu Pro 165 170 175gat att gat gtt atc att att gga tct ggt gcc ggt gct ggt gtt gtg 576Asp Ile Asp Val Ile Ile Ile Gly Ser Gly Ala Gly Ala Gly Val Val 180 185 190gcc cac act ttg gcc aac gat ggc ttc aag agt ttg gtt ttg gaa aag 624Ala His Thr Leu Ala Asn Asp Gly Phe Lys Ser Leu Val Leu Glu Lys 195 200 205ggc aaa tac ttt agc aac tcc gag ttg aac ttt gat gac aag gac ggc 672Gly Lys Tyr Phe Ser Asn Ser Glu Leu Asn Phe Asp Asp Lys Asp Gly 210 215 220gtt caa gaa tta tac caa agt gga ggt act ttg act aca gtc aac caa 720Val Gln Glu Leu Tyr Gln Ser Gly Gly Thr Leu Thr Thr Val Asn Gln225 230 235 240cag ttg ttt gtt ctt gct ggt tcc act ttt ggt ggc ggt acc act gtc 768Gln Leu Phe Val Leu Ala Gly Ser Thr Phe Gly Gly Gly Thr Thr Val 245 250 255aat tgg tca gcc tgt ctt aag acg cca ttc aag gtg cgt aag gaa tgg 816Asn Trp Ser Ala Cys Leu Lys Thr Pro Phe Lys Val Arg Lys Glu Trp 260 265 270tat gat gag ttt ggt gtt gac ttt gct gct gat gaa gca tac gat aaa 864Tyr Asp Glu Phe Gly Val Asp Phe Ala Ala Asp Glu Ala Tyr Asp Lys 275 280 285gcg cag gat tat gtt tgg cag caa atg gga gct tct acc gaa ggc atc 912Ala Gln Asp Tyr Val Trp Gln Gln Met Gly Ala Ser Thr Glu Gly Ile 290 295 300acc cac tct ttg gct aac gag att att att gaa ggt ggt aag aaa tta 960Thr His Ser Leu Ala Asn Glu Ile Ile Ile Glu Gly Gly Lys Lys Leu305 310 315 320ggt tac aag gcc aag gta tta gac caa aac agc ggt ggt cat cct cag 1008Gly Tyr Lys Ala Lys Val Leu Asp Gln Asn Ser Gly Gly His Pro Gln 325 330 335cac aga tgc ggt ttc tgt tat ttg ggc tgt aag cac ggt atc aag cag 1056His Arg Cys Gly Phe Cys Tyr Leu Gly Cys Lys His Gly Ile Lys Gln 340 345 350ggt tct gtt aat aac tgg ttt aga gac gca gct gcc cac ggt tcc cag 1104Gly Ser Val Asn Asn Trp Phe Arg Asp Ala Ala Ala His Gly Ser Gln 355 360 365ttc atg caa cag gtt aga gtt ttg caa ata ctt aac aag aag ggg atc 1152Phe Met Gln Gln Val Arg Val Leu Gln Ile Leu Asn Lys Lys Gly Ile 370 375 380gct tac ggt atc ttg tgt gag gat gtt gta acc ggc gcc aag ttc acc 1200Ala Tyr Gly Ile Leu Cys Glu Asp Val Val Thr Gly Ala Lys Phe Thr385 390 395 400att act ggc ccc aaa aag ttt gtt gtt gct gcc ggt gct ttg aac act 1248Ile Thr Gly Pro Lys Lys Phe Val Val Ala Ala Gly Ala Leu Asn Thr 405 410 415cca tct gtg ttg gtc aac tcc ggc ttc aag aac aag aac atc ggt aag 1296Pro Ser Val Leu Val Asn Ser Gly Phe Lys Asn Lys Asn Ile Gly Lys 420 425 430aac tta act ttg cac cca gtt tct gtc gtg ttt ggt gat ttt ggc aaa 1344Asn Leu Thr Leu His Pro Val Ser Val Val Phe Gly Asp Phe Gly Lys 435 440 445gac gtt caa gca gac cac ttc cac aac tcc atc atg act gcc ctt tgt 1392Asp Val Gln Ala Asp His Phe His Asn Ser Ile Met Thr Ala Leu Cys 450 455 460tca gaa gcc gct gat tta gac ggc aag ggc cat gga tgc aga att gaa 1440Ser Glu Ala Ala Asp Leu Asp Gly Lys Gly His Gly Cys Arg Ile Glu465 470 475 480acc atc ttg aac gct cca ttc atc cag gct tca ttc tta cca tgg aga 1488Thr Ile Leu Asn Ala Pro Phe Ile Gln Ala Ser Phe Leu Pro Trp Arg 485 490 495ggt agt aac gag gct aga cga gac ttg ttg cgt tac aac aac atg gtg 1536Gly Ser Asn Glu Ala Arg Arg Asp Leu Leu Arg Tyr Asn Asn Met Val 500 505 510gcg atg ttg ctc ctt agt cgt gac acc acc agt ggt tcc gtt tct gct 1584Ala Met Leu Leu Leu Ser Arg Asp Thr Thr Ser Gly Ser Val Ser Ala 515 520 525cat cca acc aaa cct gaa gct ttg gtt gtc gag tac gac gtg aac aag 1632His Pro Thr Lys Pro Glu Ala Leu Val Val Glu Tyr Asp Val Asn Lys 530 535 540ttt gac aga aac tcg atc ttg cag gca ttg ttg gtc act gct gac ttg 1680Phe Asp Arg Asn Ser Ile Leu Gln Ala Leu Leu Val Thr Ala Asp Leu545 550 555 560ttg tat atc caa ggt gcc aag aga atc ctt agt cca cag gca tgg gtg 1728Leu Tyr Ile Gln Gly Ala Lys Arg Ile Leu Ser Pro Gln Ala Trp Val 565 570 575cca att ttt gaa tcc gac aag cca aag gat aag aga tca atc aag gac 1776Pro Ile Phe Glu Ser Asp Lys Pro Lys Asp Lys Arg Ser Ile Lys Asp 580 585 590gag gac tat gtc gaa tgg aga gcc aag gtt gcc aag att cct ttc gac 1824Glu Asp Tyr Val Glu Trp Arg Ala Lys Val Ala Lys Ile Pro Phe Asp 595 600 605acc tac ggc tca cct tat ggt tcg gca cat caa atg tct tct tgc cgt 1872Thr Tyr Gly Ser Pro Tyr Gly Ser Ala His Gln Met Ser Ser Cys Arg 610

615 620atg tca ggt aag ggt cct aaa tac ggt gct gtt gac acc gat ggt aga 1920Met Ser Gly Lys Gly Pro Lys Tyr Gly Ala Val Asp Thr Asp Gly Arg625 630 635 640ttg ttt gaa tgt tcg aat gtt tat gtt gcc gat gca agt ctt ttg cca 1968Leu Phe Glu Cys Ser Asn Val Tyr Val Ala Asp Ala Ser Leu Leu Pro 645 650 655act gca agc ggt gct aat cct atg 1992Thr Ala Ser Gly Ala Asn Pro Met 66068664PRTCandida tropicalis 68Leu Met Leu Leu Cys Asp Gly Ile Ile His Glu Thr Thr Val Asp Gln1 5 10 15Ile Lys Asp Val Ile Ala Pro Asp Phe Pro Ala Asp Lys Tyr Glu Glu 20 25 30Tyr Val Arg Thr Phe Thr Lys Pro Ser Glu Thr Pro Gly Phe Arg Glu 35 40 45Thr Val Tyr Asn Thr Val Asn Ala Asn Thr Thr Asp Ala Ile His Gln 50 55 60Phe Ile Ile Leu Thr Asn Val Leu Ala Ser Arg Val Leu Ala Pro Ala65 70 75 80Leu Thr Asn Ser Leu Thr Pro Ile Lys Asp Met Ser Leu Glu Asp Arg 85 90 95Glu Lys Leu Leu Ala Ser Trp Arg Asp Ser Pro Ile Ala Ala Lys Arg 100 105 110Lys Leu Phe Arg Leu Val Ser Thr Leu Thr Leu Val Thr Phe Thr Arg 115 120 125Leu Ala Asn Glu Leu His Leu Lys Ala Ile His Tyr Pro Gly Arg Glu 130 135 140Asp Arg Glu Lys Ala Tyr Glu Thr Gln Glu Ile Asp Pro Phe Lys Tyr145 150 155 160Gln Phe Met Glu Lys Pro Lys Phe Asp Gly Ala Glu Leu Tyr Leu Pro 165 170 175Asp Ile Asp Val Ile Ile Ile Gly Ser Gly Ala Gly Ala Gly Val Val 180 185 190Ala His Thr Leu Ala Asn Asp Gly Phe Lys Ser Leu Val Leu Glu Lys 195 200 205Gly Lys Tyr Phe Ser Asn Ser Glu Leu Asn Phe Asp Asp Lys Asp Gly 210 215 220Val Gln Glu Leu Tyr Gln Ser Gly Gly Thr Leu Thr Thr Val Asn Gln225 230 235 240Gln Leu Phe Val Leu Ala Gly Ser Thr Phe Gly Gly Gly Thr Thr Val 245 250 255Asn Trp Ser Ala Cys Leu Lys Thr Pro Phe Lys Val Arg Lys Glu Trp 260 265 270Tyr Asp Glu Phe Gly Val Asp Phe Ala Ala Asp Glu Ala Tyr Asp Lys 275 280 285Ala Gln Asp Tyr Val Trp Gln Gln Met Gly Ala Ser Thr Glu Gly Ile 290 295 300Thr His Ser Leu Ala Asn Glu Ile Ile Ile Glu Gly Gly Lys Lys Leu305 310 315 320Gly Tyr Lys Ala Lys Val Leu Asp Gln Asn Ser Gly Gly His Pro Gln 325 330 335His Arg Cys Gly Phe Cys Tyr Leu Gly Cys Lys His Gly Ile Lys Gln 340 345 350Gly Ser Val Asn Asn Trp Phe Arg Asp Ala Ala Ala His Gly Ser Gln 355 360 365Phe Met Gln Gln Val Arg Val Leu Gln Ile Leu Asn Lys Lys Gly Ile 370 375 380Ala Tyr Gly Ile Leu Cys Glu Asp Val Val Thr Gly Ala Lys Phe Thr385 390 395 400Ile Thr Gly Pro Lys Lys Phe Val Val Ala Ala Gly Ala Leu Asn Thr 405 410 415Pro Ser Val Leu Val Asn Ser Gly Phe Lys Asn Lys Asn Ile Gly Lys 420 425 430Asn Leu Thr Leu His Pro Val Ser Val Val Phe Gly Asp Phe Gly Lys 435 440 445Asp Val Gln Ala Asp His Phe His Asn Ser Ile Met Thr Ala Leu Cys 450 455 460Ser Glu Ala Ala Asp Leu Asp Gly Lys Gly His Gly Cys Arg Ile Glu465 470 475 480Thr Ile Leu Asn Ala Pro Phe Ile Gln Ala Ser Phe Leu Pro Trp Arg 485 490 495Gly Ser Asn Glu Ala Arg Arg Asp Leu Leu Arg Tyr Asn Asn Met Val 500 505 510Ala Met Leu Leu Leu Ser Arg Asp Thr Thr Ser Gly Ser Val Ser Ala 515 520 525His Pro Thr Lys Pro Glu Ala Leu Val Val Glu Tyr Asp Val Asn Lys 530 535 540Phe Asp Arg Asn Ser Ile Leu Gln Ala Leu Leu Val Thr Ala Asp Leu545 550 555 560Leu Tyr Ile Gln Gly Ala Lys Arg Ile Leu Ser Pro Gln Ala Trp Val 565 570 575Pro Ile Phe Glu Ser Asp Lys Pro Lys Asp Lys Arg Ser Ile Lys Asp 580 585 590Glu Asp Tyr Val Glu Trp Arg Ala Lys Val Ala Lys Ile Pro Phe Asp 595 600 605Thr Tyr Gly Ser Pro Tyr Gly Ser Ala His Gln Met Ser Ser Cys Arg 610 615 620Met Ser Gly Lys Gly Pro Lys Tyr Gly Ala Val Asp Thr Asp Gly Arg625 630 635 640Leu Phe Glu Cys Ser Asn Val Tyr Val Ala Asp Ala Ser Leu Leu Pro 645 650 655Thr Ala Ser Gly Ala Asn Pro Met 660

Claims

1. A mutant Candida tropicalis cell, which has, compared with a wild type Candida tropicalis cell, a reduced activity of at least one of enzyme that is encoded by an intron-free nucleic acid sequence selected from groups A) and B) A) Seq ID No. 1, Seq ID No. 3, Seq ID No. 5, Seq ID No. 7, Seq ID No. 9, Seq ID No. 11, Seq ID No. 13, Seq ID No. 15, Seq ID No. 17, Seq ID No. 19, Seq ID No. 21, Seq ID No. 23, Seq ID No. 25, Seq ID No. 27, Seq ID No. 29, Seq ID No. 31, Seq ID No. 33, Seq ID No. 35, Seq ID No. 37, Seq ID No. 39, Seq ID No. 41, Seq ID No. 43, Seq ID No. 45, Seq ID No. 47, Seq ID No. 49, Seq ID No. 51, Seq ID No. 53, Seq ID No. 55, Seq ID No. 57, Seq ID No. 59, Seq ID No. 61, Seq ID No. 63, Seq ID No. 65 and Seq ID No. 67 B) a sequence that is 80% identical to at least to one sequence selected from the group consisting of Seq ID No. 1, Seq ID No. 3, Seq ID No. 5, Seq ID No. 7, Seq ID No. 9, Seq ID No. 11, Seq ID No. 13, Seq ID No. 15, Seq ID No. 17, Seq ID No. 19, Seq ID No. 21, Seq ID No. 23, Seq ID No. 25, Seq ID No. 27, Seq ID No. 29, Seq ID No. 31, Seq ID No. 33, Seq ID No. 35, Seq ID No. 37, Seq ID No. 39, Seq ID No. 41, Seq ID No. 43, Seq ID No. 45, Seq ID No. 47, Seq ID No. 49, Seq ID No. 51, Seq ID No. 53, Seq ID No. 55, Seq ID No. 57, Seq ID No. 59, Seq ID No. 61, Seq ID No. 63, Seq ID No. 65 and Seq ID No. 67.

2. The Candida tropicalis cell according to claim 1, wherein the decrease in enzymatic activity is achieved by modification of the nucleic acid sequence in the cell, wherein the modification is selected from the group consisting of insertion of foreign DNA into the nucleic acid sequence in the cell, deletion of at least parts of the nucleic acid sequence in the cell, a point mutation in the nucleic acid sequence in the cell, subjecting the nucleic acid sequence in the cell to RNA interference and exchanging a part of the nucleic acid sequence in the cell with foreign DNA.

3. The Candida tropicalis cell according to claim 2, wherein the foreign DNA is a selection marker gene.

4. The Candida tropicalis cell according to claim 1, wherein the cell is blocked at least partially in its β-oxidation.

5. The Candida tropicalis cell according to claim 1, which is derived from a strain selected from the group consisting of Candida tropicalis H41, Candida tropicalis H41B, Candida tropicalis H51, Candida tropicalis H45, Candida tropicalis H43, Candida tropicalis H53, Candida tropicalis H534, Candida tropicalis 534B, Candida tropicalis H435, Candida tropicalis ATCC20962 and Candida tropicalis HDC100.

6. The Candida tropicalis cell according to claim 5, which is derived from Candida tropicalis ATCC20962 or Candida tropicalis HDC100.

7. A method for producing ω-hydroxycarboxylic acid or ω-hydroxycarboxylic acid ester, the method comprising a) contacting the Candida tropicalis cell according to claim 1 with a medium comprising a carboxylic acid or a carboxylic acid ester, b) cultivating the cell under conditions to form the corresponding ω-hydroxycarboxylic acid or ω-hydroxycarboxylic acid esters from the carboxylic acid or the carboxylic acid ester and c) optionally isolating the ω-hydroxycarboxylic acid or ω-hydroxycarboxylic acid esters that formed.

8. A method of producing a C. tropicalis cell according to claim 1, the method comprising: I) preparing a C. tropicalis cell and II) modifying at least one gene comprising one of the sequences selected from the nucleic acid sequence groups A) and B) stated in claim 1 by insertion of foreign DNA into the gene, deletion at least of a part of the gene, a point mutation in the gene sequence, subjecting the gene to RNA interference and exchanging a part of the gene with foreign DNA.

9. The method according to claim 7, wherein the ω-hydroxycarboxylic acid or ω-hydroxycarboxylic acid ester is a ω-hydroxycarboxylic acid or ω-hydroxycarboxylic acid ester with a chain length of the carboxylic acid from 6 to 24 carbon atoms and a chain length of the alcohol component of the ester from 1 to 4 carbon atoms

10. The method according to claim 7, wherein the ω-hydroxycarboxylic acid or ω-hydroxycarboxylic acid ester is a 12-hydroxydodecanoic acid or 12-hydroxydodecanoic acid methyl ester.

11. A method according to claim 7, wherein the Candida tropicalis cells are derived from a strain selected from the group consisting of Candida tropicalis H41, Candida tropicalis H41B, Candida tropicalis H51, Candida tropicalis H45, Candida tropicalis H43, Candida tropicalis H53, Candida tropicalis H534, Candida tropicalis 534B, Candida tropicalis H435, Candida tropicalis ATCC20962 and Candida tropicalis HDC100 and wherein the cells are at least partially blocked in their β-oxidation.

12. A method of manufacturing a polymer, the method comprising polymerizing the ω-hydroxycarboxylic acid or of the ω-hydroxycarboxylic acid ester obtained by the method according to claim 7.

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