Patent application number | Description | Published |
20080299625 | Microbial Strains Producing Sphingoid Bases or Derivatives Thereof - The present invention provides microbial strains, in particular yeast strains, that produce at least 0.1 mg per g biomass dry weight of a sphingoid base. The present invention further provides a method to obtain sphingoid base-producing microbial strains comprising incubating a population of microbial cells in the presence of a suitable concentration of a toxin, selecting cells that are resistant against said toxin, and isolating cells out of the toxin-resistant cell population that produce at least 0.1 mg per g biomass dry weight of the sphingoid base of Formula I. Optionally, the method further comprises subjecting a population of toxin-resistant microbial cells that produce at least 0.1 mg per g biomass dry weight of the sphingoid base of Formula I to DNA-mediated transformation with a polynucleotide encoding an enzyme of the sphingolipid metabolic pathway. The present invention further provides a polypeptide having dihydroceramide desaturase activity obtainable form Pichia ciferrii. | 12-04-2008 |
20100190219 | PRODUCTION OF SPHINGOID BASES USING GENETICALLY ENGINEERED MICROBIAL STRAINS - The present invention provides genetically engineered microbial strains, in particular genetically engineered yeast strains, that produce at least 0.5 mg per g CDW of a sphingoid base according to Formula I or a salt or ester thereof. The present invention provides a method to obtain genetically engineered microbial strains producing at least 0.5 mg per g CDW of a sphingoid base according to Formula I or a salt or ester thereof. The method comprises the steps of: a) increasing the expression of a polynucleotide encoding an enzyme having ceramide synthase activity and/or an enzyme having ceramidase activity, the latter being capable of preferentially, or even specifically, hydrolyzing ceramides containing a sphingoid base according to Formula I, and/or b) decreasing the expression of a polynucleotide encoding an enzyme having sphingolipid Δ8-desaturase activity and/or an enzyme having ceramidase activity, the latter being capable of preferentially, or even specifically, hydrolyzing ceramides containing phytosphingosine or dihydrosphingosine as sphingoid base, and isolating strains with the required productivity. | 07-29-2010 |
20100261237 | FERMENTATIVE PRODUCTION OF ACETONE FROM RENEWABLE RESOURCES BY MEANS OF NOVEL METABOLIC PATHWAY - The invention describes a process for preparing acetone starting from acetyl-coenzyme A comprising process steps A. enzymatic conversion of acetyl-CoA into acetoacetyl-CoA B. enzymatic conversion of acetoacetyl-CoA into acetoacetate and CoA and C. decarboxylation of acetoacetate to acetone and CO | 10-14-2010 |
20110118433 | CANDIDA TROPICALIS CELLS AND USE THEREOF - The invention relates to genetically engineered | 05-19-2011 |
20110171702 | RECOMBINANT CELL PRODUCING 2-HYDROXYISOBUTYRIC ACID - The invention relates to a cell which has been genetically modified so as to be capable of producing more 2-hydroxyisobutyric acid or more polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units than its wild type, characterized in that 2-hydroxyisobutyric acid or polyhydroxyalkanoates containing 2-hydroxyisobutyric acid monomer units are produced via acetoacetyl-coenzyme A as intermediate and 3-hydroxybutyryl-coenzyme A as precursor. | 07-14-2011 |
20120034665 | ENZYMATIC METHOD FOR PRODUCING ALDEHYDES - The invention relates to methods for producing aldehydes and the oxidation and reduction products thereof. | 02-09-2012 |
20120264182 | USE OF A PROTEIN HOMOLOGOUS TO A MEAB PROTEIN FOR INCREASING THE ENZYMATIC ACTIVITY OF A 3-HYDROXYCARBOXYLIC ACID-COA MUTASE - The invention relates to the use of a protein homologous to a MeaB protein for increasing the enzymatic activity of a 3-hydroxycarboxylic acid-CoA mutase, a fusion protein comprising a 3-hydroxycarboxylic acid-CoA mutase and a protein sequence homologous to a MeaB protein and an enzymatic method for producing 2-hydroxyisobutryric acid. | 10-18-2012 |
20130035403 | CELLS, NUCLEIC ACIDS, ENZYMES AND USE THEREOF, AND METHODS FOR THE PRODUCTION OF SOPHOROLIPIDS - The invention relates to cells, nucleic acids, and enzymes, the use thereof for producing sophorolipids, and methods for producing sophorolipids. | 02-07-2013 |
20130130319 | CELLS AND METHODS FOR PRODUCING RHAMNOLIPIDS - The invention relates to cells and nucleic acids and also use thereof for producing rhamnolipids, and also methods for producing rhamnolipids. | 05-23-2013 |
20130164797 | PROCESS FOR THE IMPROVED SEPARATION OF A HYDROPHOBIC ORGANIC SOLUTION FROM AN AQUEOUS CULTURE MEDIUM - A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyses one reaction of β-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyses one of the reactions of the β-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof. | 06-27-2013 |
20130183725 | CANDIDA TROPICALIS CELLS AND USE THEREOF - The invention relates to genetically engineered | 07-18-2013 |
20140141478 | PROCESS FOR REACTING A CARBOXYLIC ACID ESTER - The invention provides a process for reacting a carboxylic acid ester of the formula (I) | 05-22-2014 |
20140178948 | PRODUCTION OF OMEGA-AMINO FATTY ACIDS - The invention provides a whole cell catalyst which expresses a recombinant α-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the α-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a α-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase. | 06-26-2014 |
20140186905 | BIOTECHNOLOGICAL SYNTHESIS PROCESS OF ORGANIC COMPOUNDS WITH THE AID OF AN ALKL GENE PRODUCT - Subject matter of the invention is a biotechnological process for the production of organic compounds with the aid of at least one alkL gene product. | 07-03-2014 |
20140199736 | PICHIA CIFERRII CELLS AND USE THEREOF - The invention relates to genetically modified | 07-17-2014 |
20140242646 | OXIDATION AND AMINATION OF PRIMARY ALCOHOLS - The present invention relates to a method comprising the steps a) providing a primary alcohol of the formula HO—(CH | 08-28-2014 |
20140256904 | BIOTECHNOLOGICAL SYNTHESIS PROCESS OF OMEGA-FUNCTIONALIZED CARBON ACIDS AND CARBON ACID ESTERS FROM SIMPLE CARBON SOURCES - The subject of the invention is a biotechnological process for the production of ω-functionalized carboxylic acids and of ω-functionalized carboxylate esters from simple carbon sources. | 09-11-2014 |
20140296125 | DETERGENT FORMULATION FOR TEXTILES, COMPRISING RHAMNOLIPIDS WITH A PREDOMINANT CONTENT OF DI-RHAMNOLIPIDS - The invention relates to detergent formulations for textiles, comprising rhamnolipids, where the content of di-rhamnolipids predominates, and to the use of certain rhamnolipid mixture compositions and of the aforementioned detergent formulations for increasing the rate of foam formation and/or for foam stabilization, and to the use of rhamnolipids for preventing the greying of a textile. | 10-02-2014 |
20140296168 | MIXTURE COMPOSITION COMPRISING RHAMNOLIPIDS - The invention relates to a mixture composition comprising rhamnolipids, to a process for its preparation, to its use for producing formulations and to formulations comprising this mixture composition. | 10-02-2014 |
20140308717 | OXIDATION AND AMINATION OF SECONDARY ALCOHOLS - The present invention relates to a method comprising the steps a) providing a secondary alcohol, b) oxidizing the secondary alcohol by contacting it with an NAD(P) | 10-16-2014 |
20150056658 | CELLS, NUCLEIC ACIDS, ENZYMES AND USE THEREOF, AND METHODS FOR THE PRODUCTION OF SOPHOROLIPIDS - The invention relates to cells, nucleic acids, and enzymes, the use thereof for producing sophorolipids, and methods for producing sophorolipids. | 02-26-2015 |
20150056659 | CELLS, NUCLEIC ACIDS, ENZYMES AND USE THEREOF, AND METHODS FOR THE PRODUCTION OF SOPHOROLIPIDS - The invention relates to cells, nucleic acids, and enzymes, the use thereof for producing sophorolipids, and methods for producing sophorolipids. | 02-26-2015 |
20150056660 | CELLS, NUCLEIC ACIDS, ENZYMES AND USE THEREOF, AND METHODS FOR THE PRODUCTION OF SOPHOROLIPIDS - The invention relates to cells, nucleic acids, and enzymes, the use thereof for producing sophorolipids, and methods for producing sophorolipids. | 02-26-2015 |
20150056661 | CELLS, NUCLEIC ACIDS, ENZYMES AND USE THEREOF, AND METHODS FOR THE PRODUCTION OF SOPHOROLIPIDS - The invention relates to cells, nucleic acids, and enzymes, the use thereof for producing sophorolipids, and methods for producing sophorolipids. | 02-26-2015 |