Patent application number | Description | Published |
20110287500 | CYTOSOLIC ISOBUTANOL PATHWAY LOCALIZATION FOR THE PRODUCTION OF ISOBUTANOL - The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and/or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 11-24-2011 |
20120288910 | METHODS OF INCREASING DIHYDROXY ACID DEHYDRATASE ACTIVITY TO IMPROVE PRODUCTION OF FUELS, CHEMICALS, AND AMINO ACIDS - The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 11-15-2012 |
20130288377 | COMPOSITIONS AND METHODS FOR MODULATING THE SENSITIVITY OF CELL TO AHAS INHIBITORS - Methods and materials useful for modulating the sensitivity of cells to an inhibitor of acetohydroxyacid synthase (AHAS) are disclosed. For example, nucleic acid molecules encoding AHAS large subunits are disclosed as well as methods for using such nucleic acid molecules to transform microbial cells and plant cells, and to confer modulated sensitivity to AHAS-inhibiting compounds onto such cells. Further provided are materials and methods useful for modulating growth, development, activity, and characteristics of host cells and organisms. | 10-31-2013 |
20140308710 | PEG-MEDIATED ASSEMBLY OF NUCLEIC ACID MOLECULES - The present invention discloses methods for assembling a nucleic acid molecule from a set of overlapping oligonucleotides. The method involves contacting a set of overlapping oligonucleotides with a DNA polymerase, a mixture of dNTPs, and a crowding agent to form an assembly mixture. In one embodiment the crowding agent is polyethylene glycol (PEG). The presence of the crowding agent facilitates the nucleic acid assembly process of the invention. The assembly mixture is then subjected to multiple cycles, each cycle comprising an annealing phase, an extension phase, and a denaturation phase, and the desired nucleic acid molecule is thereby assembled. In some embodiments one or more of the phases are time varied. | 10-16-2014 |
20140308721 | Cytosolic Isobutanol Pathway Localization for the Production of Isobutanol - The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and/or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 10-16-2014 |
20140363892 | REGULATORY ELEMENTS AND USES THEREOF - The present application provides novel regulatory elements including promoter sequences from marine microorganisms. The application further discloses DNA constructs containing these novel regulatory elements; transgenic cells, transgenic non-human organisms, and progeny containing these novel regulatory elements. Methods of modifying, producing, and using the regulatory elements are also disclosed. The regulatory elements disclosed herein are particularly suited for use in | 12-11-2014 |
Patent application number | Description | Published |
20100143997 | ENGINEERED MICROORGANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS - The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention. | 06-10-2010 |
20110020889 | YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD - The present invention provides recombinant mircoorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 01-27-2011 |
20110183392 | YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD - There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical. | 07-28-2011 |
20110201073 | Reduced by-product accumulation for improved production of isobutanol - The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 08-18-2011 |
20110201090 | Yeast microorganisms with reduced by-product accumulation for improved production of fuels, chemicals, and amino acids - The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 08-18-2011 |
20110275129 | Reduced by-product accumulation for improved production of isobutanol - The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 11-10-2011 |
20110318799 | YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD - There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical. | 12-29-2011 |
20120028323 | YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD - The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 02-02-2012 |
20120028324 | ENGINEERED MICROORGANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS - The present invention is generlly provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention. | 02-02-2012 |
20120045809 | Engineered Microorganisms for the Production of One or More Target Compounds - The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms comprise isobutanol producing metabolic pathway with one or more isobutanol pathway enzymes localized in the mitochondria. In various embodiments described herein, the recombinant microorganisms may be Crabtree-negative yeast microorganisms, microorganisms of the | 02-23-2012 |
20120058532 | ENGINEERED MICROORGANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS - The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention. | 03-08-2012 |
20120190089 | ENGINEERED MICROOGRANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS - The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention. | 07-26-2012 |
20130183730 | RECOVERY OF HIGHER ALCOHOLS FROM DILUTE AQUEOUS SOLUTIONS - This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs. | 07-18-2013 |
20130252298 | Yeast Organism Producing Isobutanol at a High Yield - The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 09-26-2013 |
20140212953 | YEAST MICROORGANISMS WITH REDUCED BY-PRODUCT ACCUMULATION FOR IMPROVED PRODUCTION OF FUELS, CHEMICALS, AND AMINO ACIDS - The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 07-31-2014 |
Patent application number | Description | Published |
20090106256 | VIRTUAL COMPUTING ENVIRONMENTS - Methods, systems, and apparatus, including computer program products, for discovering entities in a first portion of a data center network, examining information associated with the discovered entities to determine relationships that exist between pairs of entities in the first portion of the data center network, and generating a specification of the relationships that exist between pairs of entities in the first portion of the data center network based on results of the examining. | 04-23-2009 |
20090106424 | PROCESSING REQUESTS IN VIRTUAL COMPUTING ENVIRONMENTS - Methods, systems, and apparatus, including computer program products, for processing read and write requests received from a cloned instance of a server application, the cloned instance of the server application having a corresponding instance that is hosted in a production portion of a computing environment. | 04-23-2009 |
20090150885 | APPLIANCES IN VIRTUAL COMPUTING ENVIRONMENTS - Methods, systems, and apparatus, including computer program products, for reconciling data on a set of virtual machines, generating a virtual machine snapshot of the set of shadow virtual machines, identifying a set of virtual disks and containers of the set of virtual disks, wherein the set of virtual disks operates as storage for the set of shadow virtual machines, producing a set of storage snapshots of the containers of the set of virtual disks, identifying data that has changed within the set of storage snapshots, and reconciling the data back to when the virtual machine snapshot was generated. Using the reconciled data to test a computer application having a first instance, generate a cloned instance of the application, wherein the cloned instance sources storage shared with the first instance, interact with the cloned instance to cause a result without interacting with the first instance, and monitor the result. | 06-11-2009 |
20130091275 | VIRTUAL COMPUTING ENVIRONMENTS - Methods, systems, and apparatus, including computer program products, for discovering entities in a first portion of a data center network, examining information associated with the discovered entities to determine relationships that exist between pairs of entities in the first portion of the data center network, and generating a specification of the relationships that exist between pairs of entities in the first portion of the data center network based on results of the examining. | 04-11-2013 |
20130151679 | HYBRID VIRTUAL COMPUTING ENVIRONMENTS - A computer-implemented method involves two phases. In a first phase (e.g., during a development or testing phase) a secondary computing environment is formed with secondary instances of one or more servers of a primary environment. A communication module configured to establish communication between the secondary instances of the servers in the secondary computing environment and remote computing resources (e.g., “cloud” based servers) via the communication module. The secondary instances of the servers of the primary environment are then operated in conjunction with the remote computing resources. In a second phase (e.g., a production phase), the communication module is reconfigured to establish communication between the servers of the primary environment and the remote computing resources via the communication module. The servers of the primary environment are then operated in conjunction with the remote computing resources. | 06-13-2013 |
20130166716 | VIRTUAL COMPUTING ENVIRONMENTS - Methods, systems, and apparatus, including computer program products, for discovering entities in a first portion of a data center network, examining information associated with the discovered entities to determine relationships that exist between pairs of entities in the first portion of the data center network, and generating a specification of the relationships that exist between pairs of entities in the first portion of the data center network based on results of the examining | 06-27-2013 |