| Entries |
| Document | Title | Date |
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| 20080216185 | Compositions and Methods for Genetic Manipulation and Monitoring of Cell Lines - The disclosure relates generally to stem cell biology and more specifically to genetic manipulation of stem cells. Methods and compositions using recombinational cloning techniques are disclosed which allow the construction and insertion of complex genetic constructs into embryonic and adult stem cells and progenitor cells. The methods disclosed will allow the harvesting of adult stem cells pre-engineered with integration sites to facilitate early passage genetic modification. | 09-04-2008 |
| 20080222743 | RNA interference and disease resistance in avians - The invention relates to transgenic avians whose genome contains nucleotide sequences which encode therapeutic polynucleotides that correspond to one or more certain sequences in the genome of an avian pathogen. | 09-11-2008 |
| 20080222744 | In vivo transfection in avians - The present invention provides for methods of producing transgenic avians which may include delivering a heterologous nucleic acid to oviduct tissue of an avian wherein the nucleic acid enters a cell of the oviduct tissue and is expressed. | 09-11-2008 |
| 20080244763 | LEPTIN PROMOTER POLYMORPHISMS AND USES THEREOF - The present invention relates to single nucleotide polymorphisms (SNPs) in the leptin promoter, and to methods for the identification of animals carrying specific alleles of these SNPs that are associated with circulating leptin levels, feed intake, growth rate, body weight, carcass merit and carcass composition. The present invention provides oligonucleotides that can be used as primers and/or probes to amplify and/or detect these SNPs, and provides methods for selecting and grouping animals, in particular bovines, according to genotype. | 10-02-2008 |
| 20080250517 | Methods - The production of genetically modified animals, in which the genetic modifications are engineered in somatic cells cultured in vitro by gene targeting, is described. Genetically modified cells may then used as nuclear donors to produce, inter alia, live animals. The methods described can also be used to validate loci in animal chromosomes which are suitable sites for transgene addition to cells. | 10-09-2008 |
| 20080263691 | Compositions And Methods For Regulating Cardiac Performance - The present invention relates to cardiac performance, in particular to regulating cardiac performance via recombinant troponin I (TnI) protein and nucleic acids encoding recombinant TnI. The present invention provides nucleic acids encoding gain of function TnI proteins (e.g., cTnlA164H), vectors containing such nucleic acids, host cells containing such vectors, transgenic animals carrying a gain of function TnI protein (e.g., a cTnlA164H transgene), and therapeutic agents (e.g., comprising recombinant TnI, TnI analogues, synthetic TnI, or the like) or agents for gene therapy of heart failure or disease for research and therapeutic uses. | 10-23-2008 |
| 20080313754 | Method for generating hypermutable organisms - Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. | 12-18-2008 |
| 20090083873 | SEQUENCE-SPECIFIC INHIBITION OF SMALL RNA FUNCTION - The present invention relates to the discovery of a method for inhibiting RNA silencing in a target sequence-specific manner. RNA silencing requires a set of conserved cellular factors to suppress expression of gene-encoded polypeptide. The invention provides compositions for sequence-specific inactivation of the RISC component of the RNA silencing pathway, and methods of use thereof. The RISC inactivators of the present invention enable a variety of methods for identifying and characterizing miRNAs and siRNAs, RISC-associated factors, and agents capable of modulating RNA silencing. Therapeutic methods and compositions incorporating RISC inactivators and therapeutic agents identified through use of RISC inactivators are also featured. | 03-26-2009 |
| 20090106853 | Methods and materials using signaling probes - The present invention relates to methods of isolating cells or generating cell lines using signaling probes that produce a signal upon hybridization to a target sequence. Other methods that utilize the signaling probe include methods of quantifying the level of RNA expression, methods for identifying genetic recombinational events in living cells and methods of generating a transgenic animal using the isolated cells. The invention also provides protease probes. Signaling probes and protease probes that form stem-loop structures, three-arm junction structures, and dumbbell structures are provided. | 04-23-2009 |
| 20090183269 | GENE EXPRESSION SYSTEM USING ALTERNATIVE SPLICING IN INSECTS - A polynucleotide expression system is provided that is capable of alternative splicing of RNA transcripts of a polynucleotide sequence to be expressed in an organism. | 07-16-2009 |
| 20090193534 | In vivo transfection in avians - The present invention provides for methods of producing transgenic avians which may include delivering a heterologous nucleic acid to oviduct tissue of an avian wherein the nucleic acid enters a cell of the oviduct tissue and is expressed. | 07-30-2009 |
| 20090271884 | ES Cell-Derived Mice From Diploid Host Embryo Injection - Genetically modified mice and nucleic acid constructs for making the genetically modified mice are described. A first mouse having a gene encoding an activator (such as a Cre recombinase) operably linked to a developmentally-regulated promoter (such as a Nanog promoter) is provided. A second mouse having a toxic responder gene (such as a gene encoding diphtheria toxin A) is provided, where the toxic gene is expressed only in the presence of an activator, Embryos from a mating of the first and the second mouse are provided as host embryos suitable for generating mice from donor cells introduced into the host embryos. Ablating the ICM of a mouse embryo physically, chemically, or genetically is described, as well as making F0 generation mice that are substantially or in full derived from donor cells, employing a host mouse embryo with an ablated or nonproliferating ICM. | 10-29-2009 |
| 20090288182 | GENE SILENCING - Methods are disclosed for screening for the occurrence of gene silencing (e.g., post transcriptional gene silencing) in an organism. Also provided are methods for isolating silencing agents so identified. | 11-19-2009 |
| 20100043083 | Insect chemosensory receptors and methods of use thereof - Methods for controlling insect attraction, as well as inhibiting, preventing and reducing the incidence of insect-borne disease in a subject, are described by inhibiting the expression, activity, dimerization or signaling by gustatory receptors that alter insect responsiveness to carbon dioxide. Methods for identifying agents for effecting the same by interfering with expression, activity, dimerization or signaling by gustatory receptors are also provided. | 02-18-2010 |
| 20100050281 | Identification of Genes and Their Products Which Promote Hybrid Vigour or Hybrid Debility and Uses Thereof - The present invention relates to a method of identifying candidate genes and the proteins encoded by them that are useful in the inducement of hybrid vigour, hybrid debility and/or the diagnosis, prognosis and treatment of disease. In particular, the present invention relates to a method for identifying candidate genes capable of producing hybrid vigour or hybrid debility in an animal or plant, comprising the steps of: (i) comparing the mRNA sequence of alleles of candidate genes isolated from an animal or plant which exhibits hybrid vigour or hybrid debility with the nucleotide sequences from the corresponding alleles isolated from the parents of said animal or plant; (ii) identifying mRNA sequence differences in the alleles from said animal or plant which exhibits hybrid vigour or hybrid debility which codes for amino acid sequence variation; and (iii) identifying that the amino acid sequence variation between alleles of the candidate gene in said animal or plant is encoded by mRNA sequences which are located within two or more different exons within the candidate gene. | 02-25-2010 |
| 20100138947 | Method for Producing Stem Cells or Stel Cell-Like Cells from Mammalian Embryos - The present invention relates to methods and compositions for the production and derivation of pluripotent stem cells from embryos or embryo-derived cells and therapeutic uses therefor. In particular, the present invention relates to a method for producing functional stem cells or stem cell-like cells comprising the steps of culturing an embryo or embryo-derived cells in the presence of a demethylation agent and isolating functional pluripotent cells. | 06-03-2010 |
| 20100162423 | Methods and Systems for Inferring Traits to Breed and Manage Non-Beef Livestock - Methods and systems are provided for managing non-beef livestock subjects in order to maximize their individual potential performance and the value of a product from the non-beef livestock subjects, and to maximize profits obtained in marketing the non-beef livestock subjects. The methods and systems draw an inference of a trait of a non-beef livestock subject by determining the nucleotide occurrence of at least one non-beef livestock SNP that is determined to be associated with the trait. The inference is used in methods of the present invention to establish the economic value of a non-beef livestock subject, to improve profits related to selling beef from a non-beef livestock subject; to manage non-beef livestock subjects, to sort non-beef livestock subjects; to improve the genetics of a non-beef livestock population by selecting and breeding of non-beef livestock subjects, to clone a non-beef livestock subject with a specific trait, to track meat or another commercial product of a non-beef livestock subject; and to diagnose a health condition of a non-beef livestock subject. Certain embodiments of the present invention provide methods, systems, and kits are directed to inferences of a trait related to milk or a dairy product in a livestock subject. | 06-24-2010 |
| 20100169996 | METHODS AND COMPOSITIONS FOR MODULATING THE SIRNA AND RNA-DIRECTED-DNA METHYLATION PATHWAYS - Methods to identify nucleotide sequences whose expression will enhance resistance to pathogen infection are described, as is use of such nucleotide sequences to enhance resistance with minimal side effects on development. | 07-01-2010 |
| 20100205684 | CHICKEN EMBRYONIC STEM CELL AND METHOD FOR EVALUATION THEREOF - A chicken embryonic stem cell is established, which stably has pluripotency and an ability of being differentiated into a germ cell. For evaluating on whether or not the chicken embryonic stem cell can be applied to genetic modification technique, detection is made on a protein which serves as an indicator of the ability of being differentiated into a germ cell. This provides (i) a chicken embryonic stem cell applicable to genetic modification technique and (ii) a method for evaluation of the chicken embryonic stem cell. | 08-12-2010 |
| 20100212040 | ISOLATION OF LIVING CELLS AND PREPARATION OF CELL LINES BASED ON DETECTION AND QUANTIFICATION OF PRESELECTED CELLULAR RIBONUCLEIC ACID SEQUENCES - The invention is directed to reliable and efficient detection of mRNAs as well as other RNAs in living cells and its use to identify and, if desired, separate cells based on their desired characteristics. Such methods greatly simplify and reduce the time necessary to carry out previously-known procedures, and offers new approaches as well, such as selecting cells that generate a particular protein or antisense oligonucleotide, generating cell lines that express multiple proteins, generating cell lines with knock-out of one or more protein, and others. | 08-19-2010 |
| 20100229254 | METHOD FOR TARGETED CELL ABLATION - The present technology relates to a method for causing cell death. The method comprises the step of genetically manipulating chromosomal DNA of a cell such as by, for example, using a recombinase system, to lose an autosome during cell division and wherein loss of the autosome results in death of the cell. The technology also relates to a method for selective ablation of proliferative cells within a population of cells. | 09-09-2010 |
| 20100281555 | METHOD FOR MARKING BIO-INFORMATION INTO GENOME OF ORGANISM AND ORGANISM MARKED WITH THE BIO-INFORMATION - Disclosed herein is a method for marking bio-information into the genome of an organism, an organism marked with inherent bio-information by the method and a method for reading the bio-information. The method is characterized in that inherent bio-information encoded to a DNA sequence or RNA sequence is inserted into genome of organisms through a gene delivery system. Inherent bio-information is inserted into genome of organisms, thus avoiding loss by cell culture or artificial manipulation and being widely used even for organisms whose host-vector system is not provided. Based on these features, the method has the following advantages. First, inherent bio-informtion can be clearly obtained from the organisms themselves, rather than an additional means such as catalogs. Second, when organisms developed through desperate efforts are stolen, they can be tracked down and identified. Third, when serious problems occur by overuse or misuse of organisms, the origin thereof can be clearly determined. | 11-04-2010 |
| 20100287638 | NEURAL TUMOR STEM CELLS AND METHODS OF USE THEREOF - The present invention relates to the discovery that renewable stem cell lines can be derived from tumor cells and can be cultured in vitro. Accordingly, the invention provides neural tumor stem cell lines and cells from such cell lines. Because the cell lines retain characteristics of the tumors from which they are derived, the cells can be used in screening methods for identification of potential therapeutic agents and can be used to identify genetic markers which may be predictive for development of such tumors. Finally, such cells can be used to determine an appropriate therapeutic regimen for a patient suffering from a brain tumor. Cells from a patient's brain tumor can be cultured as described herein to create a cell line, and the relative effectiveness of a therapeutic agent against the cells can be tested to determine which agent or combination of agents is most effective in treating the patient's tumor. | 11-11-2010 |
| 20100293626 | METHODS FOR IMPROVEMENT OF BIRTH RATES IN CANIDAE ON SOMATIC CELL NUCLEAR TRANSFER - The present invention relates to a method for increasing the efficiency of offspring production in producing animals belonging to the family Canidae (canines) by somatic cell nuclear transfer. More specifically, relates to a method for increasing the efficiency of production of cloned canines by a method for cloning canines comprising enucleating the oocyte of a canine to prepare an enucleated oocyte, fusing a nuclear donor cell with the enucleated oocyte to prepare a nuclear transfer embryo and transferring the nuclear transfer embryo into the oviduct of a surrogate mother, wherein the nuclear donor cell is cultured in a medium containing a specific cell cycle synchronization-inducing substance such as roscovitine in the preparation thereof. The method enables to clone canines with high efficiency, and thus can contribute to the development of studies in the fields of veterinary medicine, anthropology and medical science such as the propagation of superior canines, the conservation of rare or nearly extinct canines, xenotransplantation and disease animal models. | 11-18-2010 |
| 20100319079 | ISOLATED PROLIFERATING CELLS WITH STEM CELL PROPERTIES FROM ADULT TISSUE OF POIKILOTHERMIC VERTEBRATES, STABLE CELL CULTURES THEREOF, AND METHODS FOR THEIR PREPARATION - A method of preparing adult proliferating cells with stem cell properties includes removing tissue from pronephros or pyloric appendates of an intestine of poikilothermic vertebrates, comminuting removed tissue, cultivating comminuted tissue, and propagating cells persisting in a culture. | 12-16-2010 |
| 20110041196 | miRNA-Regulated Differentiation-Dependent Self-Deleting Cassette - Targeting constructs and methods of using them are provided for differentiation-dependent modification of nucleic acid sequences in cells and in non-human animals. Targeting constructs comprising a promoter operably linked to a recombinase are provided, wherein the promoter drives transcription of the recombinase in an differentiated cell but not an undifferentiated cell. Promoters include Blimp1, Prm1, Gata6, Gata4, Igf2, Lhx2, Lhx5, and Pax3. Targeting constructs with a cassette flanked on both sides by recombinase sites can be removed using a recombinase gene operably linked to a 3′-UTR that comprises a recognition site for an miRNA that is transcribed in undifferentiated cells but not in differentiated cells. The constructs may be included in targeting vectors, and can be used to automatically modify or excise a selection cassette from an ES cell, a non-human embryo, or a non-human animal. | 02-17-2011 |
| 20110088107 | COMPOSITIONS AND METHODS FOR DERIVING OR CULTURING PLURIPOTENT CELLS - The invention provides compositions and methods useful for deriving or culturing vertebrate ES cells. Certain inventive methods comprise deriving or culturing vertebrate ES cells using medium that comprises a compound that replaces Klf4 or c-Myc in generating iPS cells. The invention provides NOD ES cells and methods of deriving or culturing them. | 04-14-2011 |
| 20110099649 | Method for performing genetic modification under a drug-free environment and components thereof - The present invention provides a method and components thereof of performing genetic modification under a drug-free environment. The method comprises the steps of generating a trapped mammalian cell library by trapper constructs (including the element of piggyBac terminal inverted repeats (TIRs)), reporter constructs, and helper constructs (including a sequence of an internal ribosomal entry site (IRES)). The present art allows: (1) to target & identify the silenced loci; (2) to separate genes with low-level expression at certain differentiation stages; (3) to evaluate the efficiency of gene targeting in the silent or repressed loci. The present invention avoids the biased gene targeting observed in the prior arts, and eliminates the needs of introducing antibiotic genes into the host genome which may lead to a potential threat of drifting antibiotic resistant genes into environment. | 04-28-2011 |
| 20110126305 | Mutant blue fluorescent protein and method of using the same for fluorescence resonance energy transfer and blue fluorescent fish - The present invention discloses a mutant blue fluorescent protein (BFP) exhibiting, at an aerobic or anaerobic system, larger fluorescent intensity than a BFPvv D7 of SEQ ID NO:2 derived from a wild type BFP, BfgV of SEQ ID NO:1, obtained from | 05-26-2011 |
| 20110126306 | Circular Nucleic Acid Vectors, and Methods for Making and Using the Same - Circular nucleic acid vectors that provide for persistently high levels of protein expression are provided. The circular vectors of the subject invention are to characterized by being devoid of expression-silencing bacterial sequences, where in many embodiments the subject vectors include a unidirectional site-specific recombination product hybrid sequence in addition to an expression cassette. Also provided are methods of using the subject vectors for introduction of a nucleic acid, e.g., an expression cassette, into a target cell, as well as preparations for use in practicing such methods. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications. Also provided is a highly efficient and readily scalable method for producing the vectors employed in the subject methods, as well as reagents and kits/systems for practicing the same. | 05-26-2011 |
| 20110167508 | PUFA POLYKETIDE SYNTHASE SYSTEMS AND USES THEREOF - The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system. | 07-07-2011 |
| 20110179510 | METHOD FOR CULTURING AVIAN GONOCYTES - A sustained culture of isolated avian gonocytes is provided, as well as a method of making and using the same. A chimeric avian containing an isolated gonocyte and a transgenic avian produced using the chimeric avian are also provided. The cell and method may be employed to make, among other things, transgenic avian that produce a heterologous protein, e.g., a therapeutic protein. | 07-21-2011 |
| 20110209231 | TREATMENT AND PREVENTION OF INFLUENZA - The present invention relates to nucleic acid molecules comprising a double-stranded region, and nucleic acid constructs encoding therfor, that are useful for the treatment and/or prevention of influenza. In particular, the present invention relates to nucleic acid constructs encoding a double stranded RNA molecule(s) that can be used to produce transgenic poultry, for example chickens, such that they are at least less susceptible to an avian influenza infection. Also provided are nucleic acid molecules comprising a double-stranded region that can be used as a therapeutic to treat and/or prevent, for example, avian influenza in poultry. | 08-25-2011 |
| 20110219465 | Suppression of B-Cell Apoptosis in Transgenic Animals Expressing Humanized Immunoglobulin - The invention provides a novel approach to increase immunoglobulin expression in non-human transgenic animals. For instance, the invention provides a method to increase humanized immunoglobulin production in animals genetically engineered to express one or several human or humanized immunoglobulin transloci. This can be done by overexpressing the apoptosis inhibitor, i.e. a rabbit bcl-2, whose expression is driven by a B-cell specific promoter specifically in the B-cell of the animal, thereby enhancing the survival of B-cells. This invention further relates to a method for selectively enhancing the survival of exogenous B-cells, that is B-cells expressing any immunoglobulin transgene locus, over the survival of endogenous B-cells that do not express the transgene locus. Selectivity is achieved by expressing the apoptosis-inhibitor only within exogenous B-cells, that is, by coupling exogenous immunoglobulin expression with apoptosis inhibitor expression. This latter method allows for increased expression and production of the transgene encoded product(s) over the endogenously produced immunoglobulin of the transgenic animal. The invention also provides a novel apoptosis-inhibitor, rabbit bcl-2. | 09-08-2011 |
| 20110239319 | Use of Endonucleases for Inserting Transgenes Into Safe Harbor Loci - The present invention concerns the endonucleases capable of cleaving a target sequence located in a “safe harbor loci”, i.e. a loci allowing safe expression of a transgene. The present invention further concerns the use of such endonucleases for inserting transgenes into a cell, tissue or individual. | 09-29-2011 |
| 20110265198 | Genome editing of a Rosa locus using nucleases - Disclosed herein are methods and compositions for genome editing of a Rosa locus, using fusion proteins comprising a DNA binding domain and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins. | 10-27-2011 |
| 20110277049 | PRODUCTION OF CLONED OFFSPRING FROM COOLED CARCASSES - Genetic material is derived from animals post-mortem, and used in nuclear transfer processes to produce cloned embryos and live cloned animals having genetic make-ups identical to the post mortem animals. The method has particular applicability to the management and breeding of livestock, to the production of animals having desired genetic traits, and to the integration of those genetic traits into selective breeding operations. | 11-10-2011 |
| 20110289611 | RNA SEQUENCE-SPECIFIC MEDIATORS OF RNA INTERFERENCE - The present invention relates to a Drosophila in vitro system which was used to demonstrate that dsRNA is processed to RNA segments 21-23 nucleotides (nt) in length. Furthermore, when these 21-23 nt fragments are purified and added back to Drosophila extracts, they mediate RNA interference in the absence of long dsRNA. Thus, these 21-23 nt fragments are the sequence-specific mediators of RNA degradation. A molecular signal, which may be their specific length, must be present in these 21-23 nt fragments to recruit cellular factors involved in RNAi. This present invention encompasses these 21-23 nt fragments and their use for specifically inactivating gene function. The use of these fragments (or chemically synthesized oligonucleotides of the same or similar nature) enables the targeting of specific mRNAs for degradation in mammalian cells, where the use of long dsRNAs to elicit RNAi is usually not practical, presumably because of the deleterious effects of the interferon response. This specific targeting of a particular gene function is useful in functional genomic and therapeutic applications. | 11-24-2011 |
| 20120017289 | RAPID METHOD FOR GENERATING GENE KNOCK DOWN MODEL - The present invention provides a novel method of generating knock down models by electroporation of shRNA construct into the testis. The present invention provides an ethically superior, non-surgical, user friendly rapid method for the generation of permanent lines of shRNA knock down non human vertebrates. This invention is ethically superior as it does not involve any loss of animal life and drastically minimizes the production time and use of animals. Current techniques for making knockout models are cumbersome, require trained personnel, costly infrastructure and require hundreds of eggs collected after killing several females. In contrast, this method neither involves any costly infrastructure nor requires trained personnel. The invention also relates to the quick incorporation of shRNA gene construct into the germline of a species so that shRNA is inheritable. The present invention also generates in a single go a variety of knock down models differentially expressing gene specific shRNA, depending on differential shRNA gene incorporation in native genome of various male germ cells, so that there is no restriction in the choice of the gene knock down. | 01-19-2012 |
| 20120017290 | Genome editing of a Rosa locus using zinc-finger nucleases - Disclosed herein are methods and compositions for genome editing of a | 01-19-2012 |
| 20120036591 | METHODS FOR MITOCHONDRIAL DNA REPLACEMENT IN OOCYTES - Methods are provided for producing a primate oocyte in vitro. The methods include removing nuclear DNA from a recipient primate oocyte from a first primate in a manner that does not lower levels of maturation promoting factor (MPF) to form an enucleated recipient primate oocyte. The recipient primate oocyte is enucleated using a non-UV-based spindle imaging system. Nuclear genetic material or DNA including chromosomes from a donor primate oocyte arrested at metaphase II from a second primate is isolated in the form of the karyoplast and introduced into the enucleated recipient primate oocyte. Introduction of the chromosomes is performed using a fusogenic agent or electroporation to produce a hybrid oocyte. | 02-09-2012 |
| 20120042401 | METHOD FOR INTRODUCING MUTANT GENE, GENE HAVING MUTATION INTRODUCED THEREIN, CASSETTE FOR INTRODUCING MUTATION, VECTOR FOR INTRODUCING MUTATION, AND KNOCK-IN NON-HUMAN MAMMALIAN ANIMAL - Disclosed is a method for introducing a mutation into a gene, which comprises the following steps: a homologous recombination step of carrying out the homologous recombination between a target gene into which the mutation is to be introduced and a target recombinant vector, thereby substituting an exon in the target gene into which the mutation is to be introduced by a target DNA sequence in the target recombinant vector; and a mutation introduction step of carrying out the specific recombination between the target DNA sequence in the resulting target recombinant gene and a mutation introduction cassette of a mutation introduction vector carrying a mutated DNA sequence containing a mutant exon by the intervening action of Cre recombinase to substitute the target DNA sequence by the mutated DNA in the mutation introduction cassette, thereby producing a mutation-introduced gene into which the mutant DNA sequence has been introduced. The method enables the production of a knock-in non-human mammalian animal, such as a knock-in mouse, which carries the mutation-introduced gene. | 02-16-2012 |
| 20120047589 | METHOD OF DELIVERY OF NUCLEIC ACIDS TO A DEVELOPING EMBRYO - Methods to introduce genetic material, such as DNA, to embryos, are disclosed. In some embodiments, the method involves preparing the pregnant mother to receive the genetic material into a blood transport vessel which passes to the embryo, avoiding a maternal capillary bed and introducing the material under low pressure so as not to kill the pregnant animal. The effectiveness of the method is such that the nucleic acid has been expressed in all the cells of the embryo and in the postnatal mouse, including the primordial germ cells, thus making the nucleic acid germ line heritable. | 02-23-2012 |
| 20120066783 | AAV CAPSID LIBRARY AND AAV CAPSID PROTEINS - Recombinant adeno-associated viral (AAV) capsid proteins are provided. Methods for generating a library of recombinant adeno-associated viral capsid proteins are also provided. | 03-15-2012 |
| 20120102583 | RESISTANCE TO BACTERIAL INFECTION - The present invention provides a method of identifying an animal having a genotype associated with resistance to bacterial infection comprising the steps of: (a) providing a sample from said mammal; (b) determining the alleles at one or more markers of the SAL1 locus to identify the genotype of the marker, wherein said SAL1 locus lies between 54.0 MB to 54.8 MB of chicken Chromosome 5 or an equivalent thereof; and (c) determining whether the genotype is a genotype associated with resistance to bacterial infection. | 04-26-2012 |
| 20120151614 | VECTOR UTILIZING BORNA DISEASE VIRUS AND USE THEREOF - Disclosed is a viral vector comprising
| 06-14-2012 |
| 20120174245 | TRANSPOSITION OF MAIZE AC/DS ELEMENTS IN VERTEBRATES - The present invention is directed to the use of the maize Ac/Ds transposable elements in vertebrates. | 07-05-2012 |
| 20120192301 | METHODS AND COMPOSITIONS FOR GENE CORRECTION - Disclosed herein are methods and compositions for correction and/or mutation of genes associated with Parkinson's Disease as well as clones and animals derived therefrom. | 07-26-2012 |
| 20120204282 | METHODS AND COMPOSITIONS FOR TREATING OCCULAR DISORDERS - Disclosed herein are methods and compositions for treating ocular disorders. | 08-09-2012 |
| 20120233717 | METHOD FOR PREPARING A TRANSGENIC ANIMAL OF SIMULTANEOUS MULTIPLE-GENE EXPRESSION - A method for preparing a transgenic animal of simultaneous multiple-gene expression is provided. Additionally, a method for preparing a transgenic embryo, which introduces both phytase gene and human myxovirus resistant gene A into a target embryo, to obtain a transgenic embryo is provided. The transgenic animal of simultaneous multiple-gene expression can be achieved by transplanting the transgenic embryo into the body of a female target animal. A significant advantage of the foregoing methods, among many others, exists in that the simultaneous expression of multiple genes can be achieved in one transgenosis, which provides a convenient mean for the preparation of combined-gene transferred animals etc. | 09-13-2012 |
| 20120266266 | METHODS OF PRODUCING GENETICALLY-MODIFIED EUKARYOTIC CELLS WITH RATIONALLY-DESIGNED MEGANUCLEASES - Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research. | 10-18-2012 |
| 20120278913 | Ribozyme Effector Gene in Dengue Fever Transmission and Disease Control - Disclosed are anto-DENV ribozyme based methods and compositions useful in the inhibition and control of all Dengue fever serotypes (designated DENV 1 through 4). A group of anti-DENV Group 1 trans-splicing introns (αDENV-GrpIa) are presented that target DENV-2 NGC genomes in situ. Methods for specifically targeting a highly conserved 5′-3′ cyclization sequence (CS) region that is common to all serotypes of the DENV are provided. The anti-DENV Group 1 trans-splicing introns (αDENV-GrpIa) specifically target two different uracil bases on the positive sense genomic strand. The invention provides an RNA based approach for transgeneic suppression of DENV in transformed mosquitoes using a group of specifically designed introns that trans-splice a new RNA sequence downstream of a targeted site. The aDENV-GrpIs target DENV infected genomes and thus provide a method for inhibiting the spread of Dengue fever. An αDENV-GrpI 9v1 is presented that is designed to be active against all forms of Dengue virus, and to effectively target the DENV-2 NGC genome in a sequence specific manner | 11-01-2012 |
| 20120304323 | MATERNALLY INDUCED STERILITY IN ANIMALS - The present invention provides Maternal Sterility Constructs (MSC) and methods of producing sterile progeny lacking germ cells. Female animals carrying the MSC transgene will give rise to a sterile generation, as the MSC specifically eliminates Progenitor Germ Cells (PGCs) of her progeny. These females are called lineage ending females. Male animals carrying the MSC transgene, however, give rise to fertile progeny (assuming the male is not derived from an MSC-transgenic female). Thus, MSC transgenic males can be used to propagate the transgenic line. The invention can be advantageously applied to eliminate pest or invasive species, or to provide effective population control and improve culture performance of farmed species, such as fish and shellfish. | 11-29-2012 |
| 20130007904 | PRODUCTION OF CLONED OFFSPRING FROM COOLED CARCASSES - Genetic material is derived from animals post-mortem, and used in nuclear transfer processes to produce cloned embryos and live cloned animals having genetic make-ups identical to the post mortem animals. The method has particular applicability to the management and breeding of livestock, to the production of animals having desired genetic traits, and to the integration of those genetic traits into selective breeding operations. | 01-03-2013 |
| 20130061343 | IN VIVO GENE REGULATION BY THE COMBINATION OF KNOCK-IN-TETO SEQUENCE INTO THE GENOME AND TETRACYCLINE-CONTROLLED TRANS-SUPPRESSOR (TTS) PROTEIN - Disclosed is a FAST (Flexible Accelerated STOP TetO-knockin) system, an efficient method for manipulating gene expression in vivo to rapidly screen animal models of disease. This invention further discloses a single gene targeting event yielding 2 distinct knockin mice—STOP-tetO and tetO knockin—which permit generation of multiple strains with variable expression patterns: 1) knockout, 2) Cre-mediated rescue; 3) tTA-mediated misexpression; 4) tTA-mediated overexpression; and 5) tTS-mediated conditional knockout/knockdown. Using the FAST system, multiple gain- and loss-of-function strains can therefore be generated on a timescale not previously achievable. These strains can then be screened for clinically-relevant abnormalities. The flexibility and broad applicability of the FAST system is demonstrated by targeting several genes encoding proteins implicated in neuropsychiatric disorders: Mlc1, Neuroligin 3, the serotonin 1A receptor, and the serotonin 1B receptor. | 03-07-2013 |
| 20130104253 | CYTOPLASMIC TRANSFER TO DE-DIFFERENTIATE RECIPIENT CELLS - Methods for de-differentiating or altering the life-span of desired “recipient” cells, e.g., human somatic cells, by the introduction of cytoplasm from a more primitive, less differentiated cell type, e.g., oocyte or blastomere are provided. These methods can be used to produce embryonic stem cells and to increase the efficiency of gene therapy by allowing for desired cells to be subjected to multiple genetic modifications without becoming senescent. Such cytoplasm may be fractionated and/or subjected to subtractive hybridization and the active materials (sufficient for de-differentiation) identified and produced by recombinant methods. | 04-25-2013 |
| 20130145488 | MESOPOROUS SILICA NANOPARTICLES SUITABLE FOR CO-DELIVERY - The invention provides gold-plated mesoporous silicate bodies comprising pores and at least one agent and methods of using those bodies. | 06-06-2013 |
| 20130212725 | FUSION PROTEINS COMPRISING A DNA-BINDING DOMAIN OF A TAL EFFECTOR PROTEIN AND A NON-SPECIFIC CLEAVAGE DOMAIN OF A RESTRICTION NUCLEASE AND THEIR USE - The present invention relates to a method of modifying a target sequence in the genome of a eukaryotic cell, the method comprising the step: (a) introducing into the cell a fusion protein comprising a DNA-binding domain of a Tal effector protein and a non-specific cleavage domain of a restriction nuclease or a nucleic acid molecule encoding the fusion protein in expressible form, wherein the fusion protein specifically binds within the target sequence and introduces a double strand break within the target sequence. The present invention further relates to the method of the invention, wherein the modification of the target sequence is by homologous recombination with a donor nucleic acid sequence further comprising the step: (b) introducing a nucleic acid molecule into the cell, wherein the nucleic acid molecule comprises the donor nucleic acid sequence and regions homologous to the target sequence. The present invention also relates to a method of producing a non-human mammal or vertebrate carrying a modified target sequence in its genome. Furthermore, the present invention relates to a fusion protein comprising a DNA-binding domain of a Tal effector protein and a non-specific cleavage domain of a restriction nuclease. | 08-15-2013 |
| 20130227720 | Rationally Designed Meganucleases With Altered Sequence Specificity and DNA-Binding Affinity - Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research. | 08-29-2013 |
| 20130276159 | METHOD AND CULTURE MEDIUM FOR PREPARING MAMMALIAN OVUM OR EMBRYO IN WHICH ZONA PELLUCIDA HAS BEEN THINNED OR ELIMINATED, AND METHOD FOR FERTILIZATION USING MAMMALIAN OVUM PREPARED BY SAME METHOD - Provided are a method for preparing a mammalian ovum or embryo in which zona pellucida has been thinned or eliminated, and a method for fertilization using the mammalian ovum prepared by the aforementioned method. The method for thinning or eliminating zona pellucida of a mammalian ovum or embryo involves treating or culturing the mammalian ovum or embryo (such as an unfertilized ovum, a fertilized ovum, or an embryo in the early stages of development) in a culture medium containing a reducing agent having SH groups (such as reduced glutathione or DTT). The resulting mammalian ovum or embryo (such as an unfertilized ovum, a fertilized ovum, or an embryo in the early stages of development) in which zona pellucida has been thinned or eliminated is capable of realizing an improved fertilization rate and development rate when used for in vitro fertilization, transplantation of a fertilized ovum, or preparation of an embryo in the early stages of development used in the production of a genetically modified animal. | 10-17-2013 |
| 20130318645 | Methods and Vectors for Gene Targeting With Inducible Specific Expression - A method, called GETWISE, for targeting mouse genes is described. GETWISE is designed to increase the frequency of homologous recombination, facilitate screening, widen the applicability of engineered animals and circumvent intrinsic gene targeting problems. GETWISE utilizes the principle of modulating gene expression by targeting tetracycline-responsive elements into a specific locus. In GETWISE alleles, control of gene expression is transferred from the endogenous to a tetracycline-inducible promoter. Endogenous promoters now control expression of the reporter gene luciferase. Breeding of GETWISE carriers with tTA/rtTA carriers enables investigators to modulate gene expression in a ubiquitous or tissue-specific manner, depending on the presence of doxycycline. GETWISE enables the study of loss or gain of gene expression in any tissue of choice within a single mouse strain. GETWISE enables the analysis of the gene expression pattern with the luciferase assay. | 11-28-2013 |
| 20140020126 | MIRNA EXPRESSION VECTOR - A miRNA expression vector including SEQ ID NO. 11. The vector is capable of improving the fertility of animals by inhibiting the expression of inhibin. | 01-16-2014 |
| 20140082760 | Non-Human Animals Expressing pH-Sensitive Immunoglobulin Sequences - Genetically modified non-human animals are provided that express an immunoglobulin variable domain that comprises at least one histidine, wherein the at least one histidine is encoded by a substitution of a non-histidine codon in the germline of the animal with a hisidine codon, or the insertion of a histidine codon in a germline immunoglobulin nucleic acid sequence. Immunoglobulin genes comprising histidines in one or more CDRs, in an N-terminal region, and or in a loop 4 region are also provided. Immunoglobulin variable domains comprising one or more histidines (e.g., histidine clusters) substituted for non-antigen-binding non-histidine residues. Non-human animals that are progeny of animals comprising modified heavy chain variable loci (V, D, J segments), modified light chain variable loci (V, J segments), and rearranged germline light chain genes (VJ sequences) are also provided. Non-human animals that make immunoglobulin domains that bind antigens in a pH-sensitive manner are provided. | 03-20-2014 |
| 20140189899 | Methods of producing new types of hybrid silk and fibers using insects, animals, and plants - The present invention provides a new and improved method for producing hybrid silk and like fibers. The invention provides a method of sequence of use which has many advantages over prior art. | 07-03-2014 |
| 20140189900 | miRNA-REGULATED DIFFERENTIATION-DEPENDENT SELF-DELETING CASSETTE - Targeting constructs and methods of using them are provided for differentiation-dependent modification of nucleic acid sequences in cells and in non-human animals. Targeting constructs comprising a promoter operably linked to a recombinase are provided, wherein the promoter drives transcription of the recombinase in an differentiated cell but not an undifferentiated cell. Promoters include Blimp1, Prm1, Gata6, Gata4, Igf2, Lhx2, Lhx5, and Pax3. Targeting constructs with a cassette flanked on both sides by recombinase sites can be removed using a recombinase gene operably linked to a 3′-UTR that comprises a recognition site for an miRNA that is transcribed in undifferentiated cells but not in differentiated cells. The constructs may be included in targeting vectors, and can be used to automatically modify or excise a selection cassette from an ES cell, a non-human embryo, or a non-human animal. | 07-03-2014 |
| 20140223592 | PIG MYOSTATIN GENE LOCUS AND USES THEREOF - A pig myostatin gene locus and uses thereof are provided. Also provided includes an expression cassette comprising a promoter, a foreign gene and a following terminator; the promoter is a DNA molecule as set forth in any of 1)-4): 1) nucleotides at positions 2642-3778 starting from the 5′ end of SEQ ID NO. 1 in the sequence listing; 2) nucleotides as set forth in SEQ ID NO. 1 in the sequence listing; 3) a DNA molecule, hybridizing and having the same function with the DNA sequence as defined in 1) or 2) under stringent condition. Experiments show that the pig myostatin gene locus provides a valuable gene source for gene targeting, as well as introducing and expressing a foreign gene at this site. | 08-07-2014 |
| 20140289882 | COMPOSITIONS AND METHODS FOR RE-PROGRAMMING CELLS WITHOUT GENETIC MODIFICATION FOR REPAIRING CARTILAGE DAMAGE - The present inventions are directed to compositions and methods regarding the reprogramming of other cells (such as embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), MSCs, fibroblasts, hematopoietic stem cells, endothelian stem cells, adipocytes, chondrocytes, osteoblasts, osteoclasts and endothelial cells) into chondrogenic cells without introducing exogenous genes to the samples. In particular, the present inventions are directed to transducible materials that are capable of transducing into the biological samples but are not genes or causing genetic modifications. The present inventions also are directed to methods of reprogramming the path of biological samples or treating diseases using the tranducible compositions thereof. | 09-25-2014 |
| 20140298505 | PROTEIN HAVING NUCLEASE ACTIVITY, FUSION PROTEINS AND USES THEREOF - The present invention relates to a nucleic acid molecule encoding (I) a polypeptide having the activity of an endonuclease, which is (a) a nucleic acid molecule encoding a polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 1; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 2; (c) a nucleic acid molecule encoding an endonuclease, the amino acid sequence of which is at least 70% identical to the amino acid sequence of SEQ ID NO: 1; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 50% identical to the nucleotide sequence of SEQ ID NO: 2; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (e) wherein T is replaced by U; (II) a fragment of the polypeptide of (I) having the activity of an endonuclease. Also, the present invention relates to a vector comprising the nucleic acid molecule and a protein encoded by said nucleic acid molecule. Further, the invention relates to a method of modifying the genome of a eukaryotic cell and a method of producing a non-human vertebrate or mammal. | 10-02-2014 |
| 20140304847 | RECOMBINATION EFFICIENCY BY INHIBITION OF NHEJ DNA REPAIR - The present invention relates to a method for modifying a target sequence in the genome of a mammalian cell, the method comprising the step of introducing into a mammalian cell: a. one or more compounds that introduce double-strand breaks in said target sequence; b. one or more DNA molecules comprising a donor DNA sequence to be incorporated by homologous recombination into the genomic DNA of said mammalian cell within said target sequence, wherein said donor DNA sequence is flanked upstream by a first flanking element and downstream by a second flanking element, wherein said first and second flanking element are different and wherein each of said first and second flanking sequence are homologous to a continuous DNA sequence on either side of the double-strand break introduced by said one or more compounds of a. within said target sequence in the genome of said mammalian cell; and c. one or more compounds that decrease the activity of the non-homologous end joining (NHEJ) DNA repair complex in said mammalian cell. Further, the invention relates to a method of producing a non-human mammal carrying a modified target sequence in its genome. | 10-09-2014 |
| 20140317767 | Persistent Inheritance of Hyperdominant Traits in a Perennial Lineage - The present invention provides a perennial lineage engendered with hyperdominant traits that express consistent penetrance in all descendants of a founder organism. A genetic construct containing one or more genetic elements encoding a self-regulating feedback loop generates a regulatory RNA, polypeptide, or other gene product at or below a trigger level of concentration in a zygote and indicates with respect to the concentration level whether one or two copies of a genetic construct conferring a hyperdominant trait exist in said zygote. | 10-23-2014 |
| 20140331345 | SEQUENCE OF PORCINE ROSA26 LOCUS AND METHODS OF USING THE SAME - The present invention provides a 5 kb sequence of porcine ROSA26 locus, which can be used for site-specific integration of an exogenous gene into a pig genome. The present invention also provides a gene targeting vector with a 3′ and 5′ arm of the porcine ROSA26 sequence, wherein a target gene can be inserted between the 3′ and 5′ arm sequence. The gene targeting vector can be used to generate transgenic pigs with stable and ubiquitous expression of the target gene. | 11-06-2014 |
| 20140351966 | SHORTCUT PROCEDURE OF TRANSGENE INTEGRATION BY HYPOTONIC SHOCK INTO MALE GERMINAL CELLS FOR GENE EXPRESSION AND TRANSGENESIS - The present invention relates to a new method of gene integration in cells comprising the steps of: preparation of a suspension of poly nucleotide fragments comprising the desired gene in a hypotonic solution; administration of said suspension to cells or tissues; and maintenance of the cells or tissues in vitro or in its normal physiological condition. The invention also relates to a method of generating transgenic animal. | 11-27-2014 |
| 20140359799 | TARGETED GENE MODIFICATION USING HYBRID RECOMBINANT ADENO-ASSOCIATED VIRUS - An in vitro method of producing a mouse cell having a genetic modification at a preselected genomic target locus includes transducing into the mouse cell an effective amount of a hybrid recombinant adeno-associated virus (AAV) vector that includes an AAV targeting construct of a first serotype packaged with a variant AAV capsid protein different than a capsid protein of the first serotype. | 12-04-2014 |
| 20150040254 | Porcine CD28 receptor, gene for encoding same, and application of same - Provided is a porcine CD28 receptor molecule, which is: 1) a protein consisting of an amino acid sequence represented by SEQ ID NO:2, or 2) a protein derived from 1) by substitution, deletion or addition of one or several amino acids in the amino acid sequence represented by SEQ ID NO:2 and having equivalent activity with 1). Further provided is a gene for coding the porcine CD28 receptor, the nucleotide sequence of which is shown as SEQ ID NO:1. When the provided co-stimulating receptor CD28 is expressed specifically and highly in a T cell, the activation, proliferation and cell factor secretion activity of the T cell when stimulated by an antigen can be enhanced, thereby enhancing the acquired immune response of a host and enhancing the immune effect of a vaccine. | 02-05-2015 |
| 20150067900 | NEW REPEAT VARIABLE DIRESIDUES FOR TARGETING NUCLEOTIDES - The present invention relates to polypeptides and more particularly to Transcription Activator-Like Effector derived proteins that allow to efficiently target and/or process nucleic acids. The present invention also concerns methods to use these proteins. The present invention also relates to vectors, compositions and kits in which RVD domains and Transcription Activator-Like Effector (TALE) proteins of the present invention are used. | 03-05-2015 |
| 20150135345 | Rationally Designed Meganucleases With Altered Sequence Specificity and DNA-Binding Affinity - Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research. | 05-14-2015 |
| 20150135346 | MATERIALS AND METHODS FOR MAKING A RECESSIVE GENE DOMINANT - The subject invention provides materials and method for making a recessive gene dominant. This is accomplished by interfering with the natural mechanisms that inhibit expression of the recessive gene and/or by interfering with the expression of the naturally dominant gene. In a preferred embodiment, the method of the subject invention comprises both reducing inhibition of expression of the recessive gene and increasing inhibition of the dominant gene. | 05-14-2015 |
| 20150143563 | ISOLATION OF LIVING CELLS AND PREPARATION OF CELL LINES BASED ON DETECTION AND QUANTIFICATION OF PRESELECTED CELLULAR RIBONUCLEIC ACID SEQUENCES - The invention is directed to reliable and efficient detection of mRNAs as well as other RNAs in living cells and its use to identify and, if desired, separate cells based on their desired characteristics. Such methods greatly simplify and reduce the time necessary to carry out previously-known procedures, and offers new approaches as well, such as selecting cells that generate a particular protein or antisense oligonucleotide, generating cell lines that express multiple proteins, generating cell lines with knock-out of one or more protein, and others. | 05-21-2015 |
| 20150291978 | miRNA-REGULATED DIFFERENTIATION-DEPENDENT SELF-DELETING CASSETTE - Targeting constructs and methods of using them are provided for differentiation-dependent modification of nucleic acid sequences in cells and in non-human animals. Targeting constructs comprising a promoter operably linked to a recombinase are provided, wherein the promoter drives transcription of the recombinase in an differentiated cell but not an undifferentiated cell. Promoters include Blimp1, Prm1, Gata6, Gata4, Igf2, Lhx2, Lhx5, and Pax3. Targeting constructs with a cassette flanked on both sides by recombinase sites can be removed using a recombinase gene operably linked to a 3′-UTR that comprises a recognition site for an miRNA that is transcribed in undifferentiated cells but not in differentiated cells. The constructs may be included in targeting vectors, and can be used to automatically modify or excise a selection cassette from an ES cell, a non-human embryo, or a non-human animal. | 10-15-2015 |
| 20150337335 | Rationally-Designed Single-Chain Meganucleases With Non-Palindromic Recognition Sequences - Disclosed are rationally-designed, non-naturally-occurring meganucleases in which a pair of enzyme subunits having specificity for different recognition sequence half-sites are joined into a single polypeptide to form a functional heterodimer with a non-palindromic recognition sequence. The invention also relates to methods of producing such meganucleases, and methods of producing recombinant nucleic acids and organisms using such meganucleases. | 11-26-2015 |
| 20150353885 | METHOD TO COUNTER-SELECT CELLS OR ORGANISMS BY LINKING LOCI TO NUCLEASE COMPONENTS - The present invention relates to the field of genetic selection, where particular genetic traits or loci combinations are sought in a progeny resulting from genetic breeding. The invention provides genetic engineering solutions to select or counter-select the occurrence of genetic events. | 12-10-2015 |
| 20160002615 | RATIONALLY-DESIGNED SINGLE-CHAIN MEGANUCLEASES WITH NON-PALINDROMIC RECOGNITION SEQUENCES - Disclosed are rationally-designed, non-naturally-occurring meganucleases in which a pair of enzyme subunits having specificity for different recognition sequence half-sites are joined into a single polypeptide to form a functional heterodimer with a non-palindromic recognition sequence. The invention also relates to methods of producing such meganucleases, and methods of producing recombinant nucleic acids and organisms using such meganucleases. | 01-07-2016 |
| 20160002671 | RATIONALLY-DESIGNED SINGLE-CHAIN MEGANUCLEASES WITH NON-PALINDROMIC RECOGNITION SEQUENCES - Disclosed are rationally-designed, non-naturally-occurring meganucleases in which a pair of enzyme subunits having specificity for different recognition sequence half-sites are joined into a single polypeptide to form a functional heterodimer with a non-palindromic recognition sequence. The invention also relates to methods of producing such meganucleases, and methods of producing recombinant nucleic acids and organisms using such meganucleases. | 01-07-2016 |
| 20160046683 | METHODS AND COMPOSITIONS FOR GENE CORRECTION - Disclosed herein are methods and compositions for correction and/or mutation of genes associated with Parkinson's Disease as well as clones and animals derived therefrom. | 02-18-2016 |
| 20160046960 | METHODS AND COMPOSITIONS FOR TARGETED GENETIC MODIFICATIONS AND METHODS OF USE - Methods and compositions are provided for generating targeted genetic modifications on the Y chromosome or a challenging target locus. Compositions include an in vitro culture comprising an XY pluripotent and/or totipotent animal cell (i.e., XY ES cells or XY iPS cells) having a modification that decreases the level and/or activity of an Sry protein; and, culturing these cells in a medium that promotes development of XY F0 fertile females. Such compositions find use in various methods for making a fertile female XY non-human mammal in an F0 generation. | 02-18-2016 |
| 20160068865 | GENOME EDITING IN RATS USING ZINC-FINGER NUCLEASES - Disclosed herein are methods and compositions for genome editing of one or more loci in a rat, using fusion proteins comprising a zinc-finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins. | 03-10-2016 |
| 20160102324 | NEW COMPACT SCAFFOLD OF CAS9 IN THE TYPE II CRISPR SYSTEM - The present invention is in the field of CRISPR-Cas system for genome targeting. The present invention relates to new engineered Cas9 scaffolds and uses thereof. More particularly, the present invention relates to methods for genome targeting, cell engineering and therapeutic application. The present invention also relates to vectors, compositions and kits in which the new Cas9 scaffolds of the present invention are used. | 04-14-2016 |
| 20160122774 | A METHOD FOR PRODUCING PRECISE DNA CLEAVAGE USING CAS9 NICKASE ACTIVITY - The present invention is in the field of a method for genome engineering based on the type II CRISPR system, particularly a method for improving specificity and reducing potential off-site. The method is based on the use of nickase architectures of Cas9 and single or multiple crRNA(s) harboring two different targets lowering the risk of producing off-site cleavage. The present invention also relates to polypeptides, polynucleotides, vectors, compositions, therapeutic applications related to the method described here. | 05-05-2016 |
| 20160122780 | GENE EXPRESSION SYSTEM USING ALTERNATIVE SPLICING IN INSECTS - A polynucleotide expression system is provided that is capable of alternative splicing of RNA transcripts of a polynucleotide sequence to be expressed in an organism. | 05-05-2016 |
| 20160153005 | DELIVERY AND USE OF THE CRISPR-CAS SYSTEMS, VECTORS AND COMPOSITIONS FOR HEPATIC TARGETING AND THERAPY | 06-02-2016 |
| 20160177339 | METHODS AND COMPOSITIONS FOR TARGETED GENETIC MODIFICATION THROUGH SINGLE-STEP MULTIPLE TARGETING | 06-23-2016 |
