Entries |
Document | Title | Date |
20080207889 | Method for the Isolation of Nucleic Acids - The present invention concerns an improved method for the isolation of nucleic acids such as DNA and RNA from bacterial, plant, animal or human cells as well as from cell cultures and virus cultures. | 08-28-2008 |
20080227969 | Nucleic acid adsorbent; methods for adsorbing, removing, dissociating, and recovering nucleic acids using the same; and method for regenerating the same - A nucleic acid adsorbent in which a polymer having a constitutional unit represented by the following formula (I) is immobilized on a water-insoluble carrier is used as a nucleic acid adsorbent capable of selectively and efficiently adsorbing nucleic acids from complex mixtures, such as cell extract, | 09-18-2008 |
20080262213 | Processing Polynucleotide-Containing Samples - Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that regain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed. | 10-23-2008 |
20080269475 | Sorbent for Nucleic Acids, Comprising Acid-Activated Layer Silicate - The invention relates to a method for removing or recovering at least one nucleic acid molecule from an aqueous or alcoholic medium with the aid of a sorbent, where the sorbent includes at least one acid-activated sheet silicate, to a composition comprising the aforementioned sorbent, and to the preferred uses thereof. | 10-30-2008 |
20080281088 | Method And Device For The Isolation Of Nucleic Acid From Nucleic Acid-Comprising Material - Method for the isolation of nucleic acid from nucleic acid comprising material, comprising the following steps: a) provision of a container suitable for the isolation of nucleic acid; b) introducing the nucleic acid comprising material and a desiccant into the container; c) drying the nucleic acid comprising material in the container; d) subjecting the nucleic acid comprising material in the container to a method for the isolation of nucleic acid from the nucleic acid comprising material; e) isolation of nucleic acid. | 11-13-2008 |
20080319182 | ISOLATION AND PURIFICATION OF NUCLEIC ACIDS WITH A SOLID PHASE - Disclosed are water-soluble ionic liquids suitable for promoting adsorption of nucleic acids to a solid phase. The use thereof, particularly methods for the isolation of nucleic acids from an aqueous solution, as well as kits for performing those methods are disclosed. | 12-25-2008 |
20090018322 | Separation of different isoforms of plasmid DNA using ultrafiltration - A method of using pressure-driven ultrafiltration efficiently to separate isoforms of plasmid DNA such as is created in bioreactors known in the art. | 01-15-2009 |
20090048437 | METHOD OF PURIFYING RNA USING KOSMOTROPIC SALT - The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less. | 02-19-2009 |
20090093624 | Techniques for Linking Non-Coding and Gene-Coding Deoxyribonucleic Acid Sequences and Applications Thereof - Techniques for linking non-coding and gene coding regions of a genome are provided. In one aspect, a method of determining associations between non-coding sequences and gene coding sequences in a genome of an organism comprises the following steps. At least one conserved region is identified from one or more non-coding sequences. Additional instances of the conserved region are located in the untranslated or amino acid coding regions of one or more genes in the organism under consideration, and the conserved region is associated with the one or more biological processes in which these one or more genes participate. | 04-09-2009 |
20090093625 | APPARATUS FOR AND METHOD OF SYNTHESIZING BIOPOLYMER AND METHOD OF RECOVERING REAGENT FOR SYNTHESIZING BIOPOLYMER - An apparatus for synthesizing a biopolymer includes a reaction chamber, an outlet tube connected to the reaction chamber, a plurality of recovery tanks connected to the outlet tube, and a plurality of recovery valves configured to open or block the passageway between the outlet tube and each of the recovery tanks. | 04-09-2009 |
20090099350 | Instrument and method for nucleic acid isolation - According to this invention, an instrument and a method for nucleic acid isolation is realized, whereby it is possible to prevent, for example, adherence of a discharged solution upon dispersion of such solution to the vicinity of a nucleic acid isolation column outlet. | 04-16-2009 |
20090131649 | METHOD OF SEPARATING SMALL RNA MOLECULES USING KOSMOTROPIC SALT - Disclosed is a method of separating small RNAs of 200 nucleotides or less from larger RNAs on a solid support, using a kosmotropic salt of different concentrations. | 05-21-2009 |
20090131650 | Polynucleotide Capture Materials, and Methods of Using Same - Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as RNA and/or DNA from such samples. The RNA and/or DNA is captured by polyamidoamine (PAMAM(Generation 0)) bound to a surface, such as the surface of magnetic particles. The methods and materials have high efficiency of binding RNA and of DNA, and of release, and thereby permit quantitative determinations. | 05-21-2009 |
20090143572 | METHOD FOR SEPERATING AND PURIFYING RNA - A method for separating and purifying RNA including the steps of passing a sample solution containing a nucleic acid, a washing solution and a recovering solution through a nucleic acid-adsorbing porous membrane to adsorb nucleic acid, adsorbing, washing and recovering, in which the nucleic acid adsorbing porous membrane is a porous membrane capable of adsorbing a nucleic acid by interaction involving substantially no ionic bond, and the sample solution is obtained by a process, comprising the steps of (I) injecting a test sample containing at least one of blood and leukocyte, and further containing an anticoagulant to a container, (II) adding a hemolytic agent to the container to obtain a leukocyte pallet, (III) adding a nucleic acid-solubilizing reagent to the leukocyte pallet to obtain a mixture solution and (IV) adding a water-soluble organic solvent to the mixture solution to obtain the sample solution containing the nucleic acid. | 06-04-2009 |
20090221809 | METHOD FOR THE SORPTION OF AT LEAST ONE NUCLEIC ACID-ACTIVATED PHYLLOSILICATES - The invention relates to a method for the sorption of at least one nucleic acid molecule from a liquid medium, comprising the following steps: (a) providing a liquid medium comprising at least one nucleic acid molecule; (b) providing a layer comprising at least one acid-activated phyllosilicate, where the layer is permeable by the liquid medium, and the layer thickness is at least 1 mm; (c) passing the liquid medium with the at least one nucleic acid molecule from step (a) through the layer from step (b) for sorption of the at least one nucleic acid molecule in the layer. | 09-03-2009 |
20090240044 | PROCESS FOR THE REDUCTION OF ENDOTOXINS IN A PLASMID PREPARATION USING A CARBOHYDRATE NON-IONIC DETERGENT WITH SILICA CHROMATOGRAPHY - The present invention provides methods for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography. | 09-24-2009 |
20090275743 | METHOD OF PREPARING CARRIER TO SEPARATE NUCLEIC ACIDS, CARRIER AND MICRO CHANNEL TO SEPARATE NUCLEIC ACIDS, AND METHOD AND APPARATUS FOR SEPARATING NUCLEIC ACIDS - The present invention provides a method for producing a carrier for nucleic acid separation, the method including a step of: immobilizing capturing chains onto a porous carrier having cation-exchangeable groups thereon by ion exchange bonding, the capturing chains having a base sequence complementary to target nucleic acid chains and being modified with functional groups having positive charges. | 11-05-2009 |
20090299047 | Method for Activating a Nucleic Acid for a Polymerase Reaction - The present invention concerns a method for activating a nucleic acid for a polymerase reaction with the steps: (a) Heating a nucleic acid to a temperature of 55° C. to 80° C., (b) cooling the nucleic acid to a temperature at which a polymerase shows no substantial decrease in activity, and (c) starting the polymerase reaction by the addition of a heat-labile polymerase to the nucleic acid. | 12-03-2009 |
20090326211 | Process for concentrating and processing fluid samples - A method of treating a liquid sample having microbiological target species therein to concentrate the species and collect lysate is disclosed. The liquid sample comprises non-target microbiological particles, inorganic particles, and microbiological target species. The liquid is passed through a prefilter medium to allow the target species to pass through as filtrate and retain non-target microbiological products and inorganic particles thereon. The filtrate is contacted with a main filtration medium adapted to retain the target species thereon as retentate. The retentate is lysed to form a lysate containing target material that was enveloped within the microbiological target species. The microbiological species may comprise cell containing or viral material. Target materials comprise intracellular nucleic acids, or in the case of viral sampling, nucleic acids encased within the protein sheath or coating of the virus. | 12-31-2009 |
20100010208 | PURIFICATION OF A TRIPLE HELIX FORMATION WITH AN IMMOBILIZED OLIGONUCLEOTIDE - Method for double-stranded DNA purification, by which a solution containing said DNA in a mixture with other components is passed over a support on which is covalently coupled in oligonucleotide capable of hybridizing with a specific sequence present on said DNA to form a triple helix. | 01-14-2010 |
20100056769 | METHODS AND KIT FOR ISOLATING NUCLEIC ACIDS - The present invention is related to a method for isolating a target nucleic acid from a sample comprising said target nucleic acid, comprising the steps of mixing a sample containing said target nucleic acid with a binding solution and a nucleic acid binding matrix, binding at least part of said target nucleic acid to said nucleic acid binding matrix, wherein said nucleic acid binding matrix is treated simultaneously or has been previously treated with at least one compound comprising a metal substance selected from the group consisting of metals of the main groups 13 to 16, semimetals and transition metals for reducing non-target nucleic acid contaminations or wherein said nucleic acid binding matrix is modified with hydrophobic groups. Furthermore, respective kits and reagents are provided with the teaching of the present invention. | 03-04-2010 |
20100063266 | Method for Isolating Both Free and Protein Association DNA - A method is disclosed for isolating both free and protein-associated DNA from bodily fluids, such as urine, saliva, serum, tears, sweat, cerebral spinal fluid, and plasma. The method comprises as a first step concentrating and isolating both the free DNA and the proteins present in the bodily fluid. The proteins are then disgested in order to release the formerly protein-associated DNA from the isolated proteins. Lastly, the free and formerly protein-associated DNA can be isolated and purified. | 03-11-2010 |
20100063267 | BI-FUNCTIONAL COMPOUND POSITIVELY CHARGED AT FIRST PH AND NEGATIVELY CHARGED AT SECOND PH, SOLID SUPPORT COMPRISING THE BI-FUNCTIONAL COMPOUND, AND METHOD OF ISOLATING NUCLEIC ACID USING THE SAME - Provided are a bi-functional compound that is positively charged at a first pH and negatively charged at a second pH, a solid support having the bi-functional compound immobilized thereon, and a method of isolating a nucleic acid, including: binding the bi-functional compound with a nucleic acid at a first pH and isolating the nucleic acid from the bi-functional compound at a second pH. | 03-11-2010 |
20100087635 | PURIFICATION OF OLIGONUCLEOTIDES - A method for purifying a protected oligonucleotide comprising the steps of:
| 04-08-2010 |
20100121044 | SOLID PHASE TECHNIQUE FOR SELECTIVELY ISOLATING NUCLEIC ACIDS - A method of isolating target nucleic acid molecules from a solution comprising a mixture of different size nucleic acid molecules, in the presence or absence of other biomolecules, by selectively facilitating the adsorption of a particular species of nucleic acid molecule to the functional group-coated surface of magnetically responsive paramagnetic microparticles is disclosed. Separation is accomplished by manipulating the ionic strength and polyalkylene glycol concentration of the solution to selectively precipitate, and reversibly adsorb, the target species of nucleic acid molecule, characterized by a particular molecular size, to paramagnetic microparticles, the surfaces of which act as a bioaffinity adsorbent for the nucleic acids. The target nucleic acid is isolated from the starting mixture based on molecular size and through the removal of magnetic beads to which the target nucleic acid molecules have been adsorbed. The disclosed method provides a simple, robust and readily automatable means of nucleic acid isolation and purification which produces high quality nucleic acid molecules suitable for: capillary electrophoresis, nucleotide sequencing, reverse transcription cloning the transfection, transduction or microinjection of mammalian cells, gene therapy protocols, the in vitro synthesis of RNA probes, cDNA library construction and PCR amplification. | 05-13-2010 |
20100160619 | COMPOSITIONS AND METHODS FOR USING A SOLID SUPPORT TO PURIFY DNA - Reagents, methods and kits for the purification of DNA from biological materials are provided. | 06-24-2010 |
20100222562 | METHOD AND TEST KIT FOR THE SEPARATION, PURIFICATION AND RECYCLING OF LONG- AND SHORT-CHAIN NUCLEIC ACIDS - Long- and/or short-chain nucleic acids are separated, purified and recovered by binding the nucleic acid to a solid phase using a binding buffer, to obtain a bonded nucleic acid, and eluting of the bonded nucleic acid from the solid phase, wherein the binding buffer comprises at least one citric acid salt and at least one alcohol. | 09-02-2010 |
20100240882 | Apparatus, System and Method for Purifying Nucleic Acids - Methods and devices for isolating nucleic acids from a mixture containing such nucleic acids and extraneous matter are provided. In one embodiment, the method of the invention comprises passing the mixture through a glass frit under conditions effective to separate the nucleic acids from the extraneous matter. In a more specific embodiment, the glass frit is a sintered glass fit. | 09-23-2010 |
20100317841 | Polyelectrolyte-Coated Size-Exclusion Ion-Exchange Particles - A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided. | 12-16-2010 |
20110054161 | METHODS OF PURIFYING A NUCLEIC ACID AND FORMULATION AND KIT FOR USE IN PERFORMING SUCH METHODS - A formulation containing guanidine thiocyanate together with acetamide, one or more acetamide derivatives, or a combination of acetamide and one or more acetamide derivatives is used to purify one or more nucleic acids contained in a medium. In particular, a medium containing at least one nucleic acid is combined with a binding matrix and the formulation in order to cause the at least one nucleic acid to separate from its in vivo cellular environment and to bind to the binding matrix. The binding matrix with at least one nucleic acid bound thereto then is separated from substantially the rest of the combined medium and formulation, after which the at least one nucleic acid is eluted from the binding matrix to obtain the at least one nucleic acid in a substantially purified form. If different nucleic acids are to be selectively purified from a single medium, multiple binding matrices, each compatible with a different nucleic acid, can be used. | 03-03-2011 |
20110054162 | Silica Magnetic Particles Having a Spherical Form and a Process for Preparing the Same - The present invention relates to silica magnetic particles having a spherical form and a process for preparing the same. The silica magnetic particles prepared according to the present invention, which are silica particles that includes the magnetic particles and additionally have the functional group on the surfaces, has an advantage that the particle size distribution is uniform. Further, the silica magnetic particles prepared according to the present invention can be used as a reagent for separating biomaterials and a reagent for detecting biomaterials. | 03-03-2011 |
20110077388 | METHOD AND APPARATUS FOR ISOLATING NUCLEIC ACIDS - A method and apparatus for efficiently isolating nucleic acids from a nucleic acid-containing sample. | 03-31-2011 |
20110092691 | Method For Filtering Nucleic Acids, In Particular From Fixed Tissue - The invention relates to a method for filtering nucleic acids, to a kit for carrying out the method according to the invention and to a novel use of magnetic particles for filtering a biological sample. The method according to the invention comprises the following steps: a) the sample is held in an aqueous solution; b) lysing of the sample; c) separation of cellular debris; and d) the nucleic acids are isolated from the solution, steps (a) to (c) taking place under non-chaotropic conditions. | 04-21-2011 |
20110092692 | METHOD FOR SEPARATION OF DOUBLE-STRANDED AND SINGLE-STRANDED NUCLEIC ACIDS FROM THE SAME SAMPLE - The invention provides systems, methods and kits for the separation and/or purification of double-stranded and single-stranded nucleic acids from the same sample. The method includes first mixing a sample containing both double-stranded nucleic acid and single-stranded nucleic acid with a solution including a chaotropic salt and a non-ionic detergent to generate a mixture; then applying the mixture to a first mineral support for double-stranded nucleic acid to bind; and collecting the flow-through which contains unbound single-stranded nucleic acid. The method further includes diluting the non-ionic detergent of the flow-through, and applying the diluted flow-through to a second mineral support for the single-stranded nucleic acid to bind. Alternatively the flow-through can be mixed with a lower aliphatic alcohol prior to loading of the second column. The double-stranded and the single-stranded nucleic acids can be eluted from the mineral supports respectively. | 04-21-2011 |
20110118457 | METHOD FOR NUCLEIC ACIDS ISOLATION - The present invention provides a method for isolating nucleic acids. The method comprises: contacting a sample containing nucleic acids with a solid phase in a first aqueous solution to provide a loaded solid phase; washing the loaded solid phase with a second aqueous solution to provide a washed solid phase; and eluting the washed solid phase with a low ionic strength liquid to obtain the isolated nucleic acids. The present invention also provides a kit for practicing the present method. | 05-19-2011 |
20110130558 | METHOD FOR ISOLATING NUCLEIC ACIDS - The invention relates to a method and kits for isolating and/or purifying nucleic acids, in particular, short-chain nucleic acids, from a nucleic acid containing starting material, characterised by the following method steps: (a) bonding the nucleic acids to a nucleic acid bonding support material, wherein the starting material is brought into contact with the nucleic acid bonding support material in the presence of at least one chaotropic compound and preferably isopropanol, wherein the isopropanol is present in a concentration of ≧15% (v/v) and ≦35% (v/v), (b) optional elution of the bonded nucleic acids from the nucleic acid bonding support material. Said method is particularly suitable for the purification of foetal DNA from maternal blood | 06-02-2011 |
20110137020 | Method and Device for Purifying Nucleic Acids - The invention concerns a method for isolating and purifying nucleic acids from a sample and a device that is suitable therefore. | 06-09-2011 |
20110160446 | METHOD FOR ISOLATING SHORT-CHAIN NUCLEIC ACIDS - The invention relates to a method and kits for isolating and/or purifying nucleic acids, in particular, short-chain nucleic acids, from a nucleic acid containing starting material, characterised by the following method steps: (a) bonding the nucleic acids to a nucleic acid bonding support material, wherein the starting material is brought into contact with the nucleic acid bonding support material in the presence of at least one chaotropic compound and preferably isopropanol, wherein the isopropanol is present in a concentration of ≧25% (v/v) and ≦35% (v/v), (b) optional elution of the bonded nucleic acids from the nucleic acid bonding support material. Said method is particularly suitable for the purification of foetal DNA from maternal blood. | 06-30-2011 |
20110166338 | REAGENT, CHAOTROPIC AGENT, AND REAGENT KIT FOR AND APPLICATIONS OF ISOLATING NUCLEIC ACID BY USE OF MAGNETIC CELLULOSE MATERIAL - Disclosed are a chaotropic agent; a reagent including a chaotropic agent and a lithium salt; a reagent kit including a chaotropic agent; a chaotropic agent, a reagent, a reagent kit, and a method for isolating a nucleic acid by use of a magnetic cellulose material; a method for binding a nucleic acid to a magnetic cellulose material; a method for isolating a nucleic acid; and a method for purifying a chromosome DNA. It is required that each of the chaotropic agents, the reagents, and the reagent kits works with at least one solid-phase, magnetic cellulose-containing carrier to isolate a nucleic acid from non-nucleic acid substances. In addition, each chaotropic agent includes an alcohol substance and a substrate solution for adjusting the alcohol substance to an appropriate concentration and thereby promoting binding of the nucleic acid in a sample to the magnetic cellulose. | 07-07-2011 |
20110172408 | RECOVERY OF NUCLEIC ACIDS FROM MAGNETIC GLASS PARTICLES - The present invention is a method of separating nucleic acids using a solid phase capable of binding nucleic acids, such as magnetic glass particles, where binding of nucleic acids to the solid phase is enhanced by the presence of an ethylene-amine compound. The invention further includes a reaction mixture for isolating nucleic acids containing an ethylene-amine compound and kits for carrying out the method. | 07-14-2011 |
20110172409 | Method for nucleic acid isolation by solid phase reversible binding of nucleic acids - The invention includes a method for separating polynucleotides, such as DNA, RNA and PNA, from a solution containing polynucleotides by reversibly and non-specifically binding the polynucleotides to a solid surface having a functional amide group-coated surface. The materials containing a solid surface can be in the form of microparticles, fibers, beads, membranes, test tubes, pipette tips or microwells and can further comprise a magnetic core portion. The pH, salt and concentration of crowding reagent, such as polyethylene glycol or alcohol, of the solution is adjusted to levels which result in nucleic acid binding to the solid surface. The magnetic microparticles with bound polynucleotides are separated from the solution under mild alkaline conditions and the nucleic acids are eluted from the magnetic microparticles. Solutions having different nucleic acid concentrations can be normalized by restricting the availability of the solid phase surface. | 07-14-2011 |
20110178286 | CONSUMABLE ANALYTICAL PLASTICWARE COMPRISING HIGH-SOLUBILITY PLASTICS - Consumable analytical plasticware comprising polymers having solubility parameters of about 9.5 (cal/cm | 07-21-2011 |
20110245482 | NON-INVASIVE DETECTION OF FETAL GENETIC TRAITS - Blood plasma of pregnant women contains fetal and (generally>90%) maternal circulatory extracellular DNA. Most of said fetal DNA contains ≦500 base pairs, said maternal DNA having a greater size. Separation of circulatory extracellular DNA of <500 base pairs results in separation of fetal from maternal DNA. A fraction of a blood plasma or serum sample of a pregnant woman containing, due to size separation (e.g. by chromatography, density gradient centrifugation or nanotechnological methods), extracellular DNA substantially comprising ≦500 base pairs is useful for non-invasive detection of fetal genetic traits (including the fetal RhD gene in pregnancies at risk for HDN; fetal Y chromosome-specific sequences in pregnancies at risk for X chromosome-linked disorders; chromosomal aberrations; hereditary Mendelian genetic disorders and corresponding genetic markers; and traits decisive for paternity determination) by e.g. PCR, ligand chain reaction or probe hybridization techniques, or nucleic acid arrays. | 10-06-2011 |
20110257386 | ISOLATION OF NUCLEIC ACID - A method for isolating a nucleic acid comprises: binding the nucleic acid to a solid phase at a first pH in the presence of a binding buffer, washing the bound nucleic acid with a wash solution, and eluting the nucleic acid from the solid phase at a second pH which is higher than the first pH. The wash solution comprises a buffer with a buffering range that encompasses a pH that is higher than the first pH, and the wash solution is at a pH that is within a buffering range of the binding buffer but lower than the buffering range of the buffer of the wash solution. Solutions, compositions, and kits for use in the methods are described. | 10-20-2011 |
20110294997 | METAL ENHANCED FLUORESCENCE-BASED SENSING METHODS - The present invention relates to metallic-surface detection systems for determining target substances including free bilirubin in neonatal serum in the presence of a predominantly high background of bilirubin bound Human Serum Albumin (HSA) or sensing and isolating target nucleotide sequences wherein a fluorescence signal is enhanced by close proximity of the target substances near metallic surfaces. | 12-01-2011 |
20110301343 | METHODS AND COMPOSITIONS FOR DEPLETING ABUNDANT RNA TRANSCRIPTS - The present invention concerns a system for isolating, depleting, and/or preventing the amplification of a targeted nucleic acid, such as mRNA or rRNA, from a sample comprising targeted and nontargeted nucleic acids. | 12-08-2011 |
20120022246 | Magnetic Pigment - Magnetic particles with an outer glass surface being essentially poreless or having pores of a diameter of less then 10 nm as well as ferromagnetic particles with a glass surface are preferentially useful for the isolation of biological material from samples. They provide a quick and reliable purification. | 01-26-2012 |
20120059160 | NUCLEIC ACID PURIFICATION WITH A BINDING MATRIX - The present invention relates to methods, kits, and compositions for generating purified RNA samples and purified DNA samples. In particular, the present invention provides methods for generating a purified RNA or DNA sample from a sample containing both DNA and RNA molecules using a binding matrix that preferentially binds DNA or RNA in the presence of an acidic dilution buffer, or using a binding matrix that comprises acid zeolites, as well as compositions and kits for practicing such methods. | 03-08-2012 |
20120071643 | SYSTEM AND METHODS FOR PURIFYING BIOLOGICAL MATERIALS - Fluid sample purification systems and methods are provided for isolating molecules of interest in a fluid sample. The fluid sample purification system has a housing with a distal end and distal opening adapted for the passage of a fluid and a proximal end and proximal opening adapted for passage of a fluid. A distal retainer is located inside the housing and above the distal opening. A proximal retainer is located inside the housing between the distal retainer and the proximal opening, or is located adjacent to, in contact with, or over the proximal opening. The system also comprises adsorption material, e.g., functionalized particles, inside the housing and confined between the distal retainer and the proximal retainer. The adsorption material adsorbs undesirable material while simultaneously rejecting desirable materials. Methods are also provided for isolating molecules of interest using the fluid sample purification system. | 03-22-2012 |
20120083597 | Enrichment of Low Molecular Weight DNA - The present invention provides, among other things, a simple, reproducible, and cost-effective method for enriching fetal or other low molecular weight nucleic acids in a biological sample. In certain embodiments, methods are provided for enriching fetal nucleic acids (e.g., fetal DNAs), typically comprising steps of adding a polymer such as PEG to a heterogeneous biological sample containing fetal DNA and high molecular weight non-fetal DNA such that the PEG precipitates substantially the high molecular weight non-fetal DNA, and purifying the fetal DNA from supernatant, thereby enriching the fetal DNA. | 04-05-2012 |
20120108804 | COMPOSITIONS AND METHODS FOR PREPARING SHORT RNA MOLECULES AND OTHER NUCLEIC ACIDS - The invention provides methods of preparing nucleic acids, such as RNA molecules, of a defined size or range of sizes. The invention provides compositions, methods and kits for use in the production and preparation of small RNA molecules (including without limitation micro-RNA, siRNA, d-siRNA and e-siRNA) and other nucleic acids of various sizes. | 05-03-2012 |
20120190837 | Nanoparticles for Manipulation of Biopolymers - Materials and methods for manipulation of biopolymers, including the separation, purification, immobilization, and archival storage of biopolymers is disclosed. | 07-26-2012 |
20120283426 | Methods and Kit for Isolating Nucleic Acids - The present invention is related to a method for isolating a target nucleic acid from a sample comprising said target nucleic acid, comprising the steps of mixing a sample containing said target nucleic acid with a binding solution and a nucleic acid binding matrix, binding at least part of said target nucleic acid to said nucleic acid binding matrix, wherein said nucleic acid binding matrix is treated simultaneously or has been previously treated with at least one compound comprising a metal substance selected from the group consisting of metals of the main groups 13 to 16, semimetals and transition metals for reducing non-target nucleic acid contaminations or wherein said nucleic acid binding matrix is modified with hydrophobic groups. Furthermore, respective kits and reagents are provided with the teaching of the present invention. | 11-08-2012 |
20120289690 | NUCLEIC ACID ELUTION - This invention relates to the storage on a solid matrix of genetic material, in particular DNA that has been purified prior to the application to the solid matrix. More specifically, the invention relates to a solid matrix for the storage of purified DNA, which matrix has been treated with a solution comprising plant polysaccharide inulin. One advantage of the invention is that an increased amount of DNA can be stored in the solid matrix of the present invention.” | 11-15-2012 |
20130030165 | CHROMATOGRAPHIC DEVICE AND METHOD FOR ISOLATING AND PURIFYING NUCLEIC ACIDS - The present invention relates to a chromatographic device for isolating and purifying nucleic acids, preferably genomic DNA, by gel filtration chromatography, a method for isolating and purifying nucleic acids, preferably genomic DNA, using this device and a kit comprising this device. | 01-31-2013 |
20130035480 | FUNCTIONALIZATION AND PURIFICATION OF MOLECULES BY REVERSIBLE GROUP EXCHANGE - Embodiments of the present disclosure include methods and compositions for functionalizing molecules, such as oligonucleotides, with functional groups, including polyhistidine tags useful in affinity methods. Some embodiments include methods for modifying and purifying complex mixtures of molecules by exchange of functional tags. | 02-07-2013 |
20130102770 | METAL ENHANCED FLUORESCENCE-BASED SENSING METHODS - The present invention relates to metallic-surface detection systems for determining target substances including free bilirubin in neonatal serum in the presence of a predominantly high background of bilirubin bound Human Serum Albumin (HSA) or sensing and isolating target nucleotide sequences wherein a fluorescence signal is enhanced by close proximity of the target substances near metallic surfaces. | 04-25-2013 |
20130158247 | METHOD FOR ISOLATING AND/OR PURIFYING NUCLEIC ACID(S) - The present invention relates to a method for isolating and/or purifying one or more nucleic acid(s) from a sample, comprising the steps of essentially separating the nucleic acid(s) from the sample by binding the nucleic acid(s) to a solid phase by means of a non-chaotropic water-soluble binding ligand at a first pH (pH I), and essentially eluting the nucleic acid(s) from the solid phase at a second pH (pH II). The invention further relates to a kit for isolating and/or purifying nucleic acid(s) from a sample and/or for protecting nucleic acid(s). | 06-20-2013 |
20130211065 | PURIFICATION OF SYNTHETIC OLIGONUCLEOTIDES - This invention provides a method for purifying synthetic oligonucleotides comprising capping, polymerizing and separating any failure sequences produced during oligonucleotide synthesis. The invention also provides a method for purifying synthetic oligonucleotides comprising reacting a full length oligonucleotide with a compound to attach a polymerizable functional group to an end of the full length oligonucleotide, polymerizing the full length oligonucleotides and removing the failure sequences, and recovering the full length oligonucleotides. The invention also provides novel capping agents having a polymerizable functional group, and kits comprising at least one composition of the present invention. | 08-15-2013 |
20130237699 | METHOD AND DEVICE FOR ISOLATING AND PURIFYING DOUBLE-STRANDED NUCLEIC ACIDS - The invention relates to a chromatographic device for isolating and/or purifying double-stranded nucleic acids, preferably double-stranded DNA, from a mixture of such nucleic acids with single-stranded nucleic acids, oligonucleotides, mononucleotides, salts and/or other such impurities. The invention also relates to a method for chromatographically isolating and/or purifying same, and to a kit for this purpose. | 09-12-2013 |
20140024822 | METHOD AND SYSTEM FOR SAMPLE PREPARATION - A method for preparing a sample by utilizing a shearing force in the presence of a size stabilizer to break apart the sample to obtain nucleic acid molecules in a usable size range. Once nucleic acid molecules are obtained, magnetic entanglement particles are used to concentrate and clean the nucleic acid molecules for further testing. | 01-23-2014 |
20140094596 | PROCESS FOR MANUFACTURING A COMPOSITE SORBENT MATERIAL FOR CHROMATOGRAPHICAL SEPARATION OF BIOPOLYMERS - The present invention relates to a sorbent material for separation and purification of biopolymers, particularly nucleic acids, having a solid support substantially modified with a copolymer coating comprising aromatic monomers and crosslinking compounds and unsaturated esters or ethers preferably attached to the support via a vinylchlorsilane. The use of these materials for separation of nucleic acids, particularly a one-step isolation of DNA from lysates of different biological sources, is an object of the invention as well as a chromatographic column or cartridge at least partially filled with the sorbent material of the invention, a membrane-like device comprising the sorbent material of the invention, and a kit comprising the sorbent material of the invention in bulk or packed in chromatographic devices as well as other devices necessary for performing sample preparations. | 04-03-2014 |
20140135489 | DEVICE FOR PREPARING A SAMPLE - The invention provides a device for preparing a fluid sample, including but not limited to a sample comprising genomic DNA. | 05-15-2014 |
20140142290 | COMPOSITIONS AND METHODS FOR PREPARING SHORT RNA MOLECULES AND OTHER NUCLEICACIDS - The invention provides methods of preparing nucleic acids, such as RNA molecules, of a defined size or range of sizes. The invention provides compositions, methods and kits for use in the production and preparation of small RNA molecules (including without limitation micro-RNA, siRNA, d-siRNA and e-siRNA) and other nucleic acids of various sizes. | 05-22-2014 |
20140179908 | Chromatography column and method for isolating nucleic acid - The present invention provides a chromatography column and a method for isolating nucleic acid molecules. In one embodiment, the present invention provides a double-layer column of a first anion exchange membrane and a second serially coupled silica membrane. Upon flowing a nucleic acid-containing solution through the first anion exchange membrane, the nucleic acid binds to and then elutes from the first membrane. The eluted solution then flows serially through the second silica membrane, which the nucleic acid binds to and then elutes from. Due to this novel serial coupled double-layer principle, the present invention is particularly suitable for co-isolating RNA and DNA, for isolating nucleic acid embraced by proteins, e.g., viruses, and for isolating diluted nucleic acid in a large volume, e.g., plasma. In addition, the eluted nucleic acid is ready for downstream applications. | 06-26-2014 |
20140213780 | PROCESS FOR SEPARATION OF OLIGONUCLEOTIDE OF INTEREST FROM A MIXTURE - The present invention is directed to a method for separation of an oligonucleotide from a mixture using a biphasic mobile phase/stationary phase liquid-liquid chromatography system. A first mobile phase contains the oligonucleotide and the stationary phase contains an exchanger substance that removably binds to the target oligonucleotide. The mobile phase is caused to flow in contact with the stationary phase in a liquid-liquid chromatography apparatus such that the oligonucleotide becomes bound to the exchanger substance in the liquid stationary phase. The oligonucleotide is then displaced from the liquid stationary phase into a second liquid mobile phase by means of a displacer substance able to displace the oligonucleotide from the stationary phase into the second mobile phase. | 07-31-2014 |
20140221638 | MATRIX AND METHOD FOR PURIFYING AND/OR ISOLATING NUCLEIC ACIDS - The present invention relates to matrix materials suitable for use in purifying and/or isolating nucleic acids from a biological sample, which matrix comprises a surface comprising at least one element selected from the group consisting of Germanium, Tin and/or Lead, or at least one salt thereof, and methods related therewith. | 08-07-2014 |
20140235846 | Polyelectrolyte-Coated Ion-Exchange Particles - A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided. | 08-21-2014 |
20140235847 | METHODS, COMPOSITIONS, AND KITS FOR GENERATING rRNA-DEPLETED SAMPLES OR ISOLATING rRNA FROM SAMPLES - The present invention provides methods, compositions, and kits for generating rRNA-depleted samples and for isolating rRNA from samples. In particular, the present invention provides compositions comprising affinity-tagged antisense rRNA molecules corresponding to substantially all of at least one rRNA molecule (e.g., 28S, 26S, 25S, 18S, 5.8S and 5S eukaryotic cytoplasmic rRNA molecules, 12S and 16S eukaryotic mitochondrial rRNA molecules, and 23S, 16S and 5S prokaryotic rRNA molecules) and methods for using such compositions to generate rRNA-depleted samples or to isolate rRNA molecules from samples. | 08-21-2014 |
20140378676 | NANOFIBRILLAR CELLULOSE AS A PHASE SEPARATION AGENT - Disclosed is the use of a separation agent including fibril cellulose for phase separation, as well as to a method for the separation of at least one liquid phase from at least one phase selected from a second liquid phase, solid phase and semi-solid phase, where the method includes the steps of incorporating a separation agent including fibril cellulose to a mixture where separation of the phases is desired, followed by formation of phases and removing the phases. | 12-25-2014 |
20150119563 | METHODS AND DEVICES FOR NUCLEIC ACID PURIFICATION - The invention provides pipette tip columns and automated methods for the purification of nucleic acids such as plasmids from unclarified cell lysates containing cell debris as well as methods for making and using such columns. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit. | 04-30-2015 |
20150133649 | System and Method for Emulsion Breaking - A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution. | 05-14-2015 |
20150307870 | PREPARATION OF SILICA PARTICLES - The present invention provides a cost-efficient method for producing particles having a SiO | 10-29-2015 |
20150337291 | SPATIALLY INHOMOGENOUSLY FUNCTIONALIZED POROUS MEDIA AND METHOD FOR USE IN SELECTIVE REMOVAL OF CONTAMINANTS - Compositions and methods for separating double-stranded nucleic acids out of a mixture comprising single-stranded nucleic acids and/or dNTPs and/or enzymes. The method uses spatially inhomogeneously functionalized nanoporous materials. For example, the compositions and methods of the present invention can be used to purify DNA amplification reaction products. | 11-26-2015 |
20150361418 | Systems and Methods for Substrate Enrichment - A method of separating bead substrates includes applying an emulsion to an emulsion-breaking solution. A dispersed phase of the emulsion includes an unbound polynucleotide, a first set of bead substrates and a second set of bead substrates. The unbound polynucleotide includes a segment complementary to a coupling oligonucleotide. The first set of bead substrates includes the coupling oligonucleotide extended to include a segment complementary to a portion of the unbound polynucleotide. The second set of bead substrates includes the coupling oligonucleotide. The emulsion-breaking solution includes an interference probe having a sequence similar to the coupling oligonucleotide or complementary to the coupling oligonucleotide. The method further includes binding beads of the first set of bead substrates to separation substrates and separating unbound beads of the second set of bead substrates from the beads of the first set of bead substrates bound to the separation substrates. | 12-17-2015 |
20160010079 | COMPOSITION FOR SYNTHESIZING PROTEIN WITH REDUCED LIPOPOLYSACCHARIDE CONTAMINATION, METHOD FOR PRODUCING PROTEIN USING SAID COMPOSITION | 01-14-2016 |
20160016147 | ABSORBENT MEDIUM FOR ISOLATION OF BIOLOGICAL MOLECULES AND METHOD FOR SYNTHESIZING SAME - An absorbent medium for biological molecules separation is provided. The absorbent medium includes a scaffold made of polymeric nanofiber. The polymeric nanofiber is decorated with silica nanoparticles. | 01-21-2016 |
20160097048 | PROCESS FOR MANUFACTURING A COMPOSITE SORBENT MATERIAL FOR CHROMATOGRAPHICAL SEPARATION OF BIOPOLYMERS - The present invention relates to a sorbent material for separation and purification of biopolymers, particularly nucleic acids, having a solid support substantially modified with a copolymer coating comprising aromatic monomers and crosslinking compounds and unsaturated esters or ethers preferably attached to the support via a vinylchlorsilane. The use of these materials for separation of nucleic acids, particularly a one-step isolation of DNA from lysates of different biological sources, is an object of the invention as well as a chromatographic column or cartridge at least partially filled with the sorbent material of the invention, a membrane-like device comprising the sorbent material of the invention, and a kit comprising the sorbent material of the invention in bulk or packed in chromatographic devices as well as other devices necessary for performing sample preparations. | 04-07-2016 |
20160152970 | METHOD FOR NUCLEI STROAGE | 06-02-2016 |
20160376636 | COMPOSITIONS AND METHODS FOR SAMPLE PREPARATION - The invention relates generally to compositions and methods for disrupting cells (e.g., disrupting cellular and nuclear membranes). In particular, the invention provides modified solid surfaces (e.g., bead surfaces) and their use in disruption of cellular membranes (e.g., during cellular lysis procedures (e.g., for recovery of nucleic acid (e.g., DNA or RNA) from mechanical cell lysis)). Compositions and methods of the invention find use in a wide range of applications including molecular biology and medical science. | 12-29-2016 |
20190144847 | NON-DESTRUCTIVE SEED GENOTYPING | 05-16-2019 |