| Class / Patent application number | Description | Number of patent applications / Date published |
|---|
| 530418000 | Precipitation | 19 |
| 20080287661 | Devices and methods for reducing matrix effects - Devices and methods are provided for reducing matrix effects in protein precipitated bioanalytical samples comprising: a support, and a sorbent associated with the support capable of binding matrix interfering agents present in the bioanalytical sample, wherein the device further comprises filtering means for removing precipitated protein particles. The filtering means is a size exclusion filter or a polymeric or inorganic monolith having a maximum pore size less than or equal to the diameter of the particles to be removed from the sample, and can be integral with the sorbent or associated with the sorbent. The sorbent is characterized by sufficient selectivity between the matrix interfering agents and analytes of interest to provide retention of the matrix interfering agents while providing elution of the analytes of interest (e.g., a reversed phase or a polar modified reversed phase). Typical devices incorporating these features include luer syringe filters, individual filter cartridges, multiwell plates, pipette tips, or inline columns for multiple or single use. | 11-20-2008 |
| 20090005544 | Process for Making Soy Protein Isolates - Disclosed is a process comprising: mixing a soy protein-containing material with an aqueous medium; removing the non-solubilized solids from the slurry to form an alkaline liquid containing solubilized soy protein; adjusting the pH of the solubilized soy protein liquid to between about 3.8 and about 6.6, to precipitate soy protein from the liquid; separating the precipitated soy protein from the pH-adjusted soy protein liquid; resolubilizing the separated soy protein precipitate in an aqueous medium at a pH of from about 1.8 to 3.2; treating the resolubilized soy protein with at least one water-soluble calcium salt to form a calcium-enriched soy protein mixture; precipitating soy protein from the calcium-enriched soy protein mixture by adjusting the pH of the mixture to between about 3.8 and about 6.6; separating the precipitated soy protein from the calcium-enriched soy protein mixture; and washing to form calcium-enriched soy protein isolate having selectively reduced manganese concentrations. | 01-01-2009 |
| 20090264632 | DEVICE AND A METHOD FOR PROMOTING CRYSTALLISATION - The invention relates to a microfluidic device for promoting crystallisation of target molecules, such as proteins. The device comprises a solid structure with a top face and an opposite bottom face and with a least one liquid channel. The liquid channel comprises a target molecule solution inlet and at least two precipitant inlets. The target molecule solution inlet is in liquid communication with each of the precipitant inlets through the liquid channel. The liquid channel comprises a branching channel section adjacent to the target molecule solution inlet, crystallisation channel sections adjacent to the respective precipitant inlets and flow break channel sections arranged between the branching channel section and each of the crystallization channel sections. The liquid channel is branched from 1 to X in the branching channel section, wherein X is the number of crystallisation channel sections, and the flow break channel sections comprise a flow break arrangement capable of breaking up the liquid communication between said respective branching channel section and crystallisation channel sections. | 10-22-2009 |
| 20100087633 | Digital microfluidic method for protein extraction by precipitation from heterogeneous mixtures - A method for extracting proteins from heterogeneous fluids by precipitation using microfluidics. The method uses an automated protocol for precipitation of proteins onto surfaces, rinsing the precipitates to remove impurities, and resolubilization in buffer for further analysis. The method is compatible with proteins representing a range of different physicochemical properties, as well as with complex mixtures such as fetal bovine serum and cell lysate. In all cases, the quantitative performance (measured using a fluorescent assay for % recovery) was comparable to that of conventional techniques, which are manual and require more time. | 04-08-2010 |
| 20120116062 | TECHNOLOGY FOR PREPARATION OF MACROMOLECULAR MICROSPHERES - Microspheres are produced by contacting an aqueous solution of a protein or other macromolecule with an organic solvent and a counterion, and chilling the solution. The microspheres are useful for preparing pharmaceuticals of defined dimensions. | 05-10-2012 |
| 20120238732 | OIL EXTRACTION FROM MICROALGAE - A method of oil extraction and biomass recovery from microalgae is disclosed. Methods according to the invention extract lipids from a biomass source and concentrate protein in the solid biomass source by alcohol processing. Aqueous alcohol processing methods provide extraction and separation techniques for lipids and protein-rich biomass suitable for biofuels. | 09-20-2012 |
| 20130090459 | METAL-ASSISTED AND MICROWAVE-ACCELERATED EVAPORATIVE CRYSTALLIZATION - The present invention relates to methods for rapid crystallization of amino acids, drug molecules, proteins and DNA/peptides. One method for rapid crystallization of functional group-containing molecules selected from the group consisting of amino acids, drug molecules, proteins and DNA/peptides includes (A) providing at least one metal or metal oxide in particulate or thin film form to provide (a) selective nucleation sites for crystallization of the functional group-containing molecules due to interactions of their functional groups and metal surfaces or engineered metal surfaces and (b) a microwave-transparent medium to create a thermal gradient between the metal surfaces or engineered metal surfaces and a warmer solution containing functional group-containing molecules to be crystallized, and (B) conducting microwave heating to cause the functional group-containing molecules to be crystallized. | 04-11-2013 |
| 20150099866 | FUNCTIONAL PROTEIN DERIVED FROM ANIMAL MUSCLE TISSUE OR MECHANICALLY DEBONED MEAT AND METHOD FOR MAKING THE SAME - A process for producing a protein product for addition to raw meat wherein the source of the protein product is animal muscle or mechanically deboned meat. The animal muscle tissue is mixed with water and homogenized. Protein in the homogenate is solubilized. Solubilized homogenate is heated to a temperature required for pasteurization and/or sterilization according to known standards. The homogenate is then adjusted to a value at which the protein precipitates. The precipitate is free of bacteria and toxins and can be used as meat or added to raw meat for delivery to a consumer as uncooked meat. | 04-09-2015 |
| 530419000 | With added material | 11 |
| 20090240040 | PURIFICATION OF A BIVALENTLY ACTIVE ANTIBODY USING A NON-CHROMATOGRAPHIC METHOD - The present invention discloses a method of purifying bivalent antibodies or antibody fragments that are active at both Fab sites from a source of antibodies or antibody fragments using a non-chromatographic method that includes inducing the formation of cyclic immunoglobulin aggregates by addition of multivalent hapten to a salt solution of soluble antibodies or antibody fragments, wherein the multivalent hapten possesses a linker between the two haptens effective to prevent the binding of both haptens of the ligand to the same antibody or antibody fragment. | 09-24-2009 |
| 20090286966 | Two-Phase Precipitation of Proteins - Provided are new methods for precipitating proteins comprising (a) providing a protein solution, (b) adding a salt to the solution, (c) either (i) adjusting pH of the composition to below the pI of the protein (in cases where the method is directed towards precipitation of most or all proteins in the solution) or (ii) adjusting the pH of the composition to above the pI of the protein (in cases where keeping the target protein in solution is desired), and (d) adding an organic compound to the solution, wherein (I) in the case where the method comprises step (c)(i) a two phase solution is formed wherein at least about 75% of the protein is contained in the protein phase or (II) in the case where the method comprises step (c)(ii) the method further comprises removing precipitated impurities from the protein solution. | 11-19-2009 |
| 20090286967 | Protein precipitation method and kit - A method and kit for precipitating protein from an aqueous solution consists of a quaternary ammonium cationic surfactant and a chaotropic salt, used in combination to reversibly precipitate proteins from the aqueous solution. Precipitation separates proteins that are entrapped, non-specifically, in the quaternary ammonium cationic surfactant:chaotropic salt complex as a pellet. The precipitated protein pellet is purified further by washing with an organic solvent to remove the surfactant:chaotrope complex, leaving behind pure, concentrated proteins that can be washed to purify further the protein and/or re-dissolved in whatever buffer is most suited for any successive procedure, such as analysis. | 11-19-2009 |
| 20100093982 | DEVICE AND METHOD FOR PRECIPITATION OF PEPTIDES - The present invention relates to a method for precipitation of peptide where the mixing step of the peptide with the precipitation aid and the precipitation itself are specially separated. | 04-15-2010 |
| 20130137859 | METHODS OF CRYSTALLISING PERFORIN - A method for isolating crystals of perforin including the step of: crystallising perforin from solution at pH 6.4 to 8.0 and 20±5° C. | 05-30-2013 |
| 20150051383 | Filtration and Extraction Assembly - Among other things, in general, a two-stage filtration and extraction assembly is described, as well as methods of use thereof. | 02-19-2015 |
| 530420000 | Inorganic | 1 |
| 20150291656 | METHOD FOR REMOVAL OF DNA - A method for removing residual DNA from a fermentation broth comprising a protein of interest and a microorganism producing the protein of interest, said method comprising: a) adding a poly aluminium chloride to the fermentation broth, and b) separating the flocculated microorganism from the fermentation broth. | 10-15-2015 |
| 530421000 | Polymeric, e.g., polyethylene glycol, etc. | 4 |
| 20080214795 | Method of isolating antibodies by precipitation - Methods of isolating antibodies by precipitation are disclosed. Various precipitants that can be employed in the invention are also disclosed, with PEG being a preferred precipitant. In a representative embodiment of the invention, the pH of a solution comprising an antibody of interest is adjusted to ±0.5 pH unit of the pI of the antibody, a precipitant such as PEG is added and the antibody of interest is subsequently isolated from the resulting precipitate. The antibody can be further purified if desired or it can be resuspended in a buffer. The invention can be employed as an alternative to or in addition to chromatographic isolation methods, such as methods that employ affinity chromatography. | 09-04-2008 |
| 20110257378 | AQUEOUS TWO PHASE EXTRACTION AUGMENTED PRECIPITATION PROCESS FOR PURIFICATION OF THERAPEUTIC PROTEINS - The invention relates to an aqueous two phase extraction (ATPE) augmented precipitation process, which may be used to recover and also partially purify therapeutic proteins, including monoclonal antibodies from a crude multi-component mixture. The process involves the formation of a forward extraction PEG-Phosphate ATPE system in which the target product is preferentially partitioned to the polymer rich phase. A second ATPE back extraction system is then formed by introducing the polymer rich phase from the forward extraction to a new phosphate salt rich phase, causing the product to precipitate at the interface between the two phases. This precipitate is then recovered and resolubilised in a suitable buffer and may be passed on for further purification. | 10-20-2011 |
| 20130123476 | Purification of Proteins - The present invention relates to a selectively soluble polymer capable of binding to one or more constituents in a mixture containing various biological materials and the methods of using such a polymer to purify a biomolecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and is rendered insoluble and precipitates out of solution upon a change in the process conditions. While in its solubilized state, the polymer is capable of binding to a selected entity within the stream such as impurities (DNA, RNA, host cell protein, endotoxins, etc) in a cell broth and remains capable of binding to that entity even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered and further processed. | 05-16-2013 |
| 20130137860 | Purification Of Proteins - The present invention relates to a selectively soluble polymer capable of binding to a desired biomolecules in a mixture containing various biological materials and the methods of using such a polymer to purify a biomolecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and/or salt concentration and is rendered insoluble and precipitates out of solution upon a change in the process conditions. The polymer is capable of binding to the desired biomolecule (protein, polypeptide, etc) and remains capable of binding to that biomolecule even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered such as by elution and further processed. | 05-30-2013 |
