| Entries |
| Document | Title | Date |
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| 20080214792 | Systems and Methods For Separating Proteins From Connective Tissue - Methods and systems for separating muscle tissue from connective tissue are provided, in which animal tissue containing both muscle tissue and connective tissue is subjected to stress, and muscle proteins are separated from the connective tissue. Slurries of separated myofibrillar protein are also provided. | 09-04-2008 |
| 20080214793 | Method for extracting a biosubstance from hair and hair sampling device useful in the method - Disclosed herein is a method for extracting a biosubstance from a root of a hair, including the step of using as the hair a hair that has pulling force of at least a predetermined reference value to pull out the hair. | 09-04-2008 |
| 20080221310 | Extraction of Cellular Components with Fatty Acid Derivatives - A method of treating biologic material with a composition containing at least one fatty acid derivative and/or at least one fatty alcohol, a buffer, and a salt to result in protein partitioning into an aqueous phase and an organic phase. The protein may be a membrane protein. The cells may be plant cells and/or animal cells. The proteins can be thereafter be used for downstream applications. | 09-11-2008 |
| 20080221311 | Preparation method of insect cell extract solution for cell-free protein synthesis, the insect cell extract solution and cell-free synthesis method of protein using the insect cell extract solution - The present invention provides a preparation method of an insect cell extract solution for cell-free protein synthesis, the insect cell extract solution, a protein synthesis method in a cell-free system, which uses the insect cell extract solution, and a kit for cell-free protein synthesis containing the insect cell extract solution. The extract solution is easily prepared by the method of the present invention and can synthesize a higher amount of protein than by extract solutions prepared by conventional methods. | 09-11-2008 |
| 20080242848 | Commercial production of insulin and insulin-like proteins in plants - Plants are engineered to express proinsulin or insulin. The plants can be used as a source of the protein for a variety of purposes. Plant tissue can be orally administered to animals to replace the necessity of injection of insulin. Alternatively, the protein can be extracted from plant tissue and delivered to animals. Plant produced proteins can also provide a less expensive and more readily available source of the protein as reagents or in other experimentation involving insulin proteins. | 10-02-2008 |
| 20080262205 | Inhibitors of GSK-3 and crystal structures of GSK-3beta protein and protein complexes - The present invention relates to inhibitors of GSK-3 and methods for producing these inhibitors. The invention also provides pharmaceutical compositions comprising the inhibitors and methods of utilizing those compositions in the treatment and prevention of various disorders, such as diabetes and Alzheimer's disease. In addition, the invention relates to molecules or molecular complexes which comprise binding pockets of GSK-3β or its homologues. The invention relates to a computer comprising a data storage medium encoded with the structure coordinates of such binding pockets. The invention also relates to methods of using the structure coordinates to solve the structure of homologous proteins or protein complexes. The invention relates to methods of using the structure coordinates to screen for and design compounds that bind to GSK-3β protein or homologues thereof. The invention also relates to crystallizable compositions and crystals comprising GSK-3β protein or GSK-3β protein complexes. | 10-23-2008 |
| 20080275223 | Method of Preparing a Liquid, Containing Proteins for Subsequent Separation, by Using One or More Protein-Complexing Agents - The invention relates to a method of preparing a liquid, which contains proteins (esp. haze sensitive proteins), for subsequent separation of at least haze-forming material, when predetermined filter aids are used. The method comprises the addition of protein-complexing agent to the liquid, which agent is capable of forming a complex with at least some sensitive proteins of the liquid, to result in a limitation of the residual haze of the liquid, obtained after the separation (e.g. filtration), step. | 11-06-2008 |
| 20090023902 | Method for the extraction of intracellular proteins from a fermentation broth - A method for extracting an intracellular peptide, protein or other polypeptide from a whole fermentation broth using a water miscible alcohol, or a water miscible or partially water miscible glycol ether. | 01-22-2009 |
| 20090069549 | Protein Extraction buffer, a kit comprising it and method of its use - Disclosed are detergent-free protein extract buffers to extract proteins from formalin-fixed paraffin-embedded tissue sections, kit comprising the buffer, and methods for using the buffer to extract proteins from formalin-fixed, paraffin-embedded tissue sections. | 03-12-2009 |
| 20090076252 | Continuous process for production of oil seed protein isolate - Oil seed protein isolates, particularly canola protein isolate, are produced continuously from oil seed meals, preferably at a high purity level of at least about 100 wt % (N×6.25), by a process wherein oil seed protein is continuously extracted from oil seed meal, the resulting protein solution is continuously concentrated, preferably to a protein content of at least about 200 g/L, and the concentrated protein solution is continuously mixed with chilled water having a temperature below about 15° C. to form protein micellar, which are settled in the settling vessel to provide a protein micellar mass (PMM) while supernatant overflows the vessel. The PMM, when accumulated to a desired degree, may be separated from supernatant and dried. The supernatant may be processed to recover additional oil seed protein isolate. | 03-19-2009 |
| 20090099343 | Isolation of pathogenic prions - Peptide reagents that interact preferentially with the PrP | 04-16-2009 |
| 20090149638 | SYSTEMS AND METHODS FOR PURIFYING PROTEINS - Described herein are novel systems and downstream protein purification (DSP) processes that provide high quality product rapidly, and on a large scale. Many of the processes enable one chromatography step to follow another chromatography step without an intermediate ultrafiltration/diafiltration (UFDF) step. These optimized processes allow for automation on the manufacture plant floor, permitting the use of a multi-cycling strategies that can utilize smaller, less expensive columns. The processes can provide considerable advantage on production efficiency, cost saving and on waste disposal. | 06-11-2009 |
| 20090171073 | EUKARYOTIC EXPRESSION SYSTEM FOR THE INCORPORATION OF STABLE ISOTOPES INTO PROTEINS - Methods for producing stable isotope-labeled recombinant protein are provided. The methods include isolating a stable isotope-labeled recombinant protein from a | 07-02-2009 |
| 20090182130 | Methods of protein production using ovomucoid promoters - Methods of producing protein using a recombinant ovomucoid gene expression controlling region operably linked to one or more useful amino acid coding sequences. | 07-16-2009 |
| 20090275741 | PLANTS AND SEEDS OF CORN VARIETY CV678981 - According to the invention, there is provided seed and plants of the corn variety designated CV678981. The invention thus relates to the plants, seeds and tissue cultures of the variety CV678981, and to methods for producing a corn plant produced by crossing a corn plant of variety CV678981 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV678981 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV678981. | 11-05-2009 |
| 20090312528 | Method of Producing a Set of MHC Molecules - The present invention relates to a method of producing a set of MHC molecules, and more precisely to a method of producing a set of MHC molecules which differ in their peptide bound in the binding groove. The method allows for parallel and rapid synthesis of different MHC molecules at high yields for each molecule. | 12-17-2009 |
| 20100029914 | NOVEL PACKING MATERIAL WITH EXCELLENT HYDROPHILICITY AND PROCESS FOR PRODUCING THE SAME - To provide a medium suitable for high speed/high resolution, rich in hydrophilicity and resistant to a high concentration aqueous alkaline solution. | 02-04-2010 |
| 20100029915 | METHOD AND SYSTEM FOR SELECTIVE ISOLATION OF TARGET BIOLOGICAL MOLECULES IN A GENERAL PURPOSE SYSTEM - Embodiments of the present techniques provide systems and methods for isolating particular classes of biological molecules, for example, proteins or nucleic acids, from mixtures of biological components. The methods use solutions that react with the biological molecules to enhance their adsorption by substrates, allowing contaminants to be washed away from the targeted molecules. Embodiments include automated systems that can be used to implement the technique with no or minimal intervention. Other embodiments include separation column technologies that may be used in the techniques. | 02-04-2010 |
| 20100029916 | PROCESS FOR PRODUCTION OF BIVALIRUDIN - The invention relates to methods for the preparation of high purity Bivalirudin. The polypeptide is prepared in a high purity of at least 98.5% (by HPLC), wherein the total impurities amount to less than 1.5%, comprising not more than 0.5% [Asp | 02-04-2010 |
| 20100099855 | RECOMBINANT PROTEIN COMPRISING STARCH BINDING DOMAIN AND USE THEREOF - A recombinant protein is prepared comprising a polypeptide of interest and a starch binding domain (SBD). The said SBD is obtainable from glucoamylase of fungi genus | 04-22-2010 |
| 20100105882 | MOLECULAR MODULE - The present invention aims at providing a general-purpose experimental tool which specifically binds to a macromolecular substance that will be a receptor for a specific ligand such as drug, and is applicable throughout various processes to explore the nature of the macromolecular substance. In order to achieve this object, a molecular module has been developed which binds to a target compound and is used for purifying or labeling the target compound, wherein the molecular module has a rod-like spacer substance, an interacting substance that interacts with the target compound, a tag and a labeling substance, the interacting substance being positioned at one end of the rod-like spacer substance, and the tag and the labeling substance being positioned at the other end of the rod-like spacer substance. | 04-29-2010 |
| 20100184962 | Metal oxide nano-composite magnetic material, fabrication method, and method for linkage, enrichment, and isolation of phosphorylated species - The present invention relates to a metal oxide nano-composite magnetic material, fabrication method, and method for linkage, enrichment, and isolation of phosphorylated species. The metal oxide nano-composite magnetic material comprises the magnetic iron oxide nanoparticle, a silica layer immobilized onto the magnetic iron oxide nanoparticle and a metal oxide layer coated onto the silica layer. The magnetic iron oxide nanoparticles can be used for absorbing microwave radiation to accelerate the enrichment and linkage for phosphorylated species onto the metal oxide nano-composite magnetic material. Furthermore, the magnetic property of magnetic iron oxide nanoparticles leads to isolation of the metal oxide nano-composite magnetic material-target species conjugates by simply employing an external magnetic field. The specificity of the metal oxide on the surface of the metal oxide nano-composite magnetic material for phosphorylated species also leads to the linkage/enrichment of phosphorylated species with high selectivity. | 07-22-2010 |
| 20100184963 | QUINOA PROTEIN CONCENTRATE, PRODUCTION AND FUNCTIONALITY - The present invention relates to a new source of high quality plant protein, termed, “ | 07-22-2010 |
| 20100222558 | PROCESS FOR PROTEIN EXTRACTION - The invention includes a process for extracting a target protein from | 09-02-2010 |
| 20100249388 | PLANTS AND SEEDS OF CORN VARIETY CV251054 - According to the invention, there is provided seed and plants of the corn variety designated CV251054. The invention thus relates to the plants, seeds and tissue cultures of the variety CV251054, and to methods for producing a corn plant produced by crossing a corn plant of variety CV251054 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV251054 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV251054. | 09-30-2010 |
| 20100249389 | PLANTS AND SEEDS OF CORN VARIETY CV433540 - According to the invention, there is provided seed and plants of the corn variety designated CV433540. The invention thus relates to the plants, seeds and tissue cultures of the variety CV433540, and to methods for producing a corn plant produced by crossing a corn plant of variety CV433540 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV433540. | 09-30-2010 |
| 20100273993 | METHOD FOR THE EXTRACTION OF MEMBRANE PROTEINS - The present invention relates to the use of linear, amphipathic carbohydrate polymers for the extraction of membrane proteins from biological samples, to a method for the extraction of membrane proteins, to a kit for the extraction of these proteins, and to the use thereof. | 10-28-2010 |
| 20110003976 | REMOVAL OF LIPOPOLYSACCHARIDES FROM PROTEIN-LIPOPOLYSACCHARIDE COMPLEXES BY NON FLAMMABLE SOLVENTS - During the production of recombinant proteins from gram negative bacteria, lipopolysaccharides (LPS, endotoxin) are released along with the protein of interest. In many instances, LPS will copurify with the target protein due to specific or non-specific protein-ILPS interactions. We have investigated the ability of alkanediols to effect the separation of LPS from protein-LPS complexes while the complexes are immobilized on anion or cation exchange chromatographic media. Alkanediols provide a safer alternative to the use of other organics such as alcohols or acetonitrile due to their lower toxicity and decreased flammability. In addition, they are less costly than many of the detergents that have been used for such purposes. LPS removal efficiency increased with increasing alkane chain length. 1,2-alkanediols were more effective than terminal alkanediols in the separation of LPS from protein LPS complexes. | 01-06-2011 |
| 20110060132 | SELF-CONTAINED, HIGH EFFICIENCY CELLULOSE BIOMASS PROCESSING PLANT - A self-contained, high efficiency cellulose biomass processing plant includes sources of quantum-based wave energy to facilitate the dilute acid hydrolysis of hemi-cellulose and alpha-cellulose bond. The sources of quantum-based wave energy supply one or more of, and suitably a combination of ultrasonic waves, ultraviolet waves, magnetic waves and direct current to facilitate fracture of the intermolecular bonds. An integrated plant is also provided which combines the cellulose biomass processing plant with apparatus for converting high protein residue into a finished high grade protein product. Due to the high efficiency resulting from use of quantum-based wave energy, the plant uses less energy, and can be small and portable. | 03-10-2011 |
| 20110124845 | PROCESS FOR PROTEIN EXTRACTION - The invention includes a process for extracting a target protein from | 05-26-2011 |
| 20110130551 | Lipid Extraction From Microalgae Using A Single Ionic Liquid - A one-step process for the lysis of microalgae cell walls and separation of the cellular lipids for use in biofuel production by utilizing a hydrophilic ionic liquid, 1-butyl-3-methylimidazolium. The hydrophilic ionic liquid both lyses the microalgae cell walls and forms two immiscible layers, one of which consists of the lipid contents of the lysed cells. After mixture of the hydrophilic ionic liquid with a suspension of microalgae cells, gravity causes a hydrophobic lipid phase to move to a top phase where it is removed from the mixture and purified. The hydrophilic ionic liquid is recycled to lyse new microalgae suspensions. | 06-02-2011 |
| 20110137018 | MAGNETIC SEPARATION SYSTEM WITH PRE AND POST PROCESSING MODULES - A system for sorting and trapping magnetic target species includes a microfluidic chamber designed to receive and then temporarily hold magnetic particles in place within the module. A pre-processing module may mix a sample and magnetic particles to cause certain species in the sample to be labeled. The micorfluidic chamber may include a mechanism to move magnetic particles within the chamber. A post-processing module or the microfluidic chamber may be used to separate the labeled species from the magnetic particles by adding a release reagent. The magnetic particles and/or their payloads may be released and separately collected at an outlet of the chamber or the post-processing module. | 06-09-2011 |
| 20110213133 | Device For And Method Of Extracting A Fraction From A Biological Sample - A device and method are provided for facilitating extraction of a fraction from a biological sample. The biological sample includes non-desired material and a fraction-bound solid phase substrate. The device includes an input zone for receiving the biological sample therein and a phase-gate zone for receiving an isolation buffer therein. An output zone receives a reagent therein. A force is movable between a first position adjacent the input zone and a second position adjacent the output zone. The force urges the fraction-bound solid phase substrate from the input zone, through the phase-gate zone and into the output zone. | 09-01-2011 |
| 20110230650 | Compositions and Methods for Solubilizing Membrane Proteins Without the Use of Detergent - Disclosed are compositions and methods for isolating proteins from lipids. The isolation methods may utilize derivatives of apolipoprotein A1 for isolating membrane proteins from membranes without the use of detergent. | 09-22-2011 |
| 20110269946 | PROTEIN SEPARATION DEVICE - The invention provides a protein separation device comprising a chaperone protein immobilized on a substrate. In one embodiment, the chaperone protein is an Hsp60 chaperone, preferably a group one chaperone, preferably GroEL. The invention also provides a method for isolating a protein from a biological sample using a protein separation device of the invention. | 11-03-2011 |
| 20120142904 | PROTEIN RENATURATION MICROFLUIDIC DEVICES AND METHODS OF MAKING AND USING THE SAME - Microfluidic devices having a protein renaturation component and methods for using the same are provided. Aspects of the present disclosure include microfluidic devices that include a separation medium with a first flow path and a protein renaturation component in fluid communication with the separation medium and having a second flow path. Also provided are methods of using the devices as well as systems and kits that include the devices. The devices, systems and methods find use in a variety of different applications, including diagnostic and validation assays. | 06-07-2012 |
| 20120232253 | METHOD FOR PURIFYING FACTOR B - The invention relates to a method for purifying factor B, comprising the steps consisting in:
| 09-13-2012 |
| 20130085268 | MEANS FOR THE TRANSIENT PRODUCTION IN PLANTS OF RECOMBINANT PROTEINS THAT CAN BE USED IN PARTICULAR IN PROPHYLAXIS AND IN THERAPEUTICS - The invention provides a composition for the inoculation in a plant of agrobacteria transfected by expression vectors, in order to produce in said plant a protein of interest or a derivative of said protein, by deletion or by mutation, characterised in that it comprises
| 04-04-2013 |
| 20130116414 | METHODS OF EXTRACTING CHEMICAL COMPOUNDS FROM ORGANISMS WITH RESISTANT CELL WALLS - A method is provided for extraction of chemical compounds from an organism having a cell wall that includes adding nanomaterials, which may be metallic nanofibers such as silver nanofibers, to the organism. | 05-09-2013 |
| 20130172539 | METHODS AND COMPOSITIONS OF PREPARATION FOR PROTEOME ANALYSIS - A method of extracting a polypeptide from a biological sample includes contacting the biological sample with an extraction reagent to form a solution of the biological sample and the extraction reagent. The extraction reagent includes perfluorooctanoic and can be used at a concentration effective to solubilize the polypeptides in the biological sample. | 07-04-2013 |
| 20130324707 | SYNTHETIC AMPHIPHILES FOR MEMBRANE PROTEIN MANIPULATION - The invention provides amphiphilic compounds and methods for manipulating membrane proteins. Compounds of the invention, for example, the compounds of Formulas I-XIX, can be prepared from readily available starting materials. The amphiphilic compounds can manipulate membrane protein at relatively low concentrations compared to many known detergents. The compounds can be used to aid the isolation of membrane proteins, for example, to aid their solubilization and/or purification. The compounds can also be used to aid the functional and structural determination of membrane proteins, including their stabilization and crystallization. | 12-05-2013 |
| 20130338345 | Purification of Diphtheria Toxoid - The invention disclosed is a method of purifying Diphtheria Toxoid (DT) by Hydrophobic Interaction Chromatography (HIC). The chromatographic method of the present invention provides an effective removal of contaminating glycans present in carrier protein DT and thereby provides a highly purified form of carrier protein DT for the production or preparation of polysaccharide protein conjugate vaccines. | 12-19-2013 |
| 20140031531 | METHOD OF PROTEIN PURIFICATION - A method for producing a target protein is provided, which includes steps described below. A crude extract including a fusion protein is provided. The fusion protein includes a tag, a target protein and a linker inserted between the tag and the target protein. The fusion protein and magnetic particles are then bound to form a magnetic particle-binding fusion protein. Finally, the linker of the magnetic particle-binding fusion protein undergoes autocleavage by using a cleavage buffer solution to release the target protein. A one-pot process for producing a purified target protein is also provided. | 01-30-2014 |
| 20150315232 | Refolding Proteins Using a Chemically Controlled Redox State - A method of refolding proteins expressed in non-mammalian cells present in concentrations of 2.0 g/L or higher is disclosed. The method comprises identifying foe thiol pair ratio and the redox buffer strength to achieve conditions under which efficient folding at concentrations of 2.0 g/L or higher is achieved and can be employed over a range of volumes, including commercial scale. | 11-05-2015 |
| 20150344542 | PARTIAL PURIFICATION METHOD FOR HYDROPHILIC RECOMBINANT PROTEIN - The present invention relates to a method for partially purifying a hydrophilic recombinant protein from a host expressing a hydrophilic recombinant protein. The method includes: a solution acquisition step in which a dissolving solvent is added to the host expressing a hydrophilic recombinant protein so as to dissolve the host cell and an insoluble substance is separated therefrom so as to obtain a solution. The hydrophilic recombinant protein has a hydropathy index of 0 or less, and the dissolving solvent is an aprotic polar solvent. Preferably, the solution is subjected to solvent substitution using an aqueous solvent. | 12-03-2015 |
| 20150360180 | SINGLE-PASS FILTRATION SYSTEMS AND PROCESSES - The present invention provides, in various embodiments, hybrid single-pass tangential flow filtration assemblies, disposable single-pass tangential flow filtration assemblies, scalable single-pass tangential flow filtration assemblies and adaptable modular single-pass tangential flow filtration assemblies. In other embodiments, the invention relates to processes for recovering proteins from the surface of a filtration membrane in a single-pass tangential flow filtration assembly and for cleaning a tangential flow filtration assembly. In additional embodiments, the invention provides methods of increasing the processing capacity of a single-pass tangential flow filtration assembly. | 12-17-2015 |
| 20160159854 | Concentration and purification of hydrophobins and antibodies with a phase separation method - The present invention relates to a purification and concentration method for proteins and antibodies. Particularly the present invention relates to a continuous surfactant based phase separation method for recovering hydrophobin fusion proteins, and for recovering target molecules, such as antibodies, directly from a liquid by using phase separation and hydrophobin-Protein A fusion technologies. | 06-09-2016 |
| 20220135617 | CHROMATOGRAPHIC PURIFICATION OF AT LEAST ONE ENZYME SELECTED FROM A GROUP INCLUDING COLLAGENASE TYPE I, COLLAGENASE TYPE II, NEUTRAL PROTEASE AND CLOSTRIPAIN - The present invention relates to a method for purifying at least one enzyme selected from the group consisting of collagenase type I, collagenase type II, neutral protease and clostripain from a mixture of substances, comprising as a method step at least one hydrophobic interaction chromatography, characterized in that, in the hydrophobic interaction chromatography, the stationary phase comprises a material selected from the group consisting of polypropylene glycol and butyl sepharose. The present invention further relates to the use of an enzyme thus purified for pharmaceutical, cosmetic and/or biochemical purposes. | 05-05-2022 |
