| Entries |
| Document | Title | Date |
|---|
| 20080213893 | ISOLATION OF NEURAL STEM CELLS USING GANGLIOSIDES AND OTHER SURFACE MARKERS - During the growth and study of NSCs, a range of molecules present on the surface of multipotent neural stem and progenitor cells (NSCs) were identified. These markers were identified using a number of human and murine neural stem cell lines, including retinal stem cells (RSCs). The NSC-specific markers identified included gene products as well as non-protein molecules and sugar epitopes not directly coded in the genome. Together with surface markers which were determined to be absent from the surface of hNSCs, the molecules described herein provide a means to enrich for neural stem cells, or neural progenitor subpopulations, particularly using combinatorial cell sorting strategies. These same molecules also represent targets for pharmacological manipulation of NSC populations and subpopulations, both in vivo and ex vivo. Furthermore, these molecules provide potential targets for therapeutic manipulation of other neural precursor-related cell types including malignant conditions as well as other diseases originating from, or preferentially affecting, various uncommitted or replication-competent cell types. | 09-04-2008 |
| 20080220522 | Methods to Control Cell Movement in Hollow Fiber Bioreactors - This invention is directed toward methods of moving cells throughout a hollow fiber bioreactor using fluid flow. | 09-11-2008 |
| 20080233649 | PANCREATIC STEM CELLS - Pancreatic progenitor cells isolated from the pancreas of a mammal. The invention also includes pancreatic cells or neural cells differentiated from the pancreatic progenitor cells. | 09-25-2008 |
| 20080261305 | TGF-beta-MEDIATED OSTEOGENIC DIFFERENTIATION OF MESENCHYMAL STEM CELLS - Methods and compositions are provided for the culture of MSC to provide osteogenic progenitor cells. | 10-23-2008 |
| 20080318317 | Kit for Preparing a Composition Comprising Fat Cells - A kit for preparing a composition including fat cells includes: a) at least one sterile tube ( | 12-25-2008 |
| 20090011506 | APPARATUS AND PROCESS FOR WASHING TISSUE AND/OR CELL - An apparatus for washing cells and/or tissue includes an outer sleeve, an inner assembly, and a filter. The outer sleeve includes a closed end and an open end. The inner assembly is axially movable in the outer sleeve. The filter is affixed to the inner assembly, wherein a chamber is formed by the filter, the outer sleeve, and the inner assembly. Furthermore, a process for washing cells and/or tissue comprising: (a) providing an apparatus as described above, wherein the cells and/or tissue and washing liquid are disposed in the chamber, and (b) axially moving the inner assembly to wash the cells and/or tissue. | 01-08-2009 |
| 20090075380 | Methods and Compositions To Enhance Efficiency Of Nuclear Transfer/Cloning - The present invention provides compositions and methods for increasing the success of assisted reproductive technology (ART). Specifically, the inventions described herein increase the survival rate of manipulated embryos for increasing post implantation numbers of viable offspring. In particular, the present invention provides for compositions and methods for allowing further embryonic development and increasing rates of embryonic maturation, such as increasing cleavage rate, TE numbers, and blastocyte formation of in vitro fertilized and nuclear transfer embryos in media comprising follistatin, thereby providing for increased survival of fertilized and manipulated embryos leading to increased numbers of live offspring from in vitro fertilized and implanted nuclear transfer embryos. Further provided are diagnostic kits for determining transplantation potential. | 03-19-2009 |
| 20090075381 | Amnion-derived cell compositions, methods of making and uses thereof - The invention is directed to substantially purified amnion-derived cell populations, compositions comprising the substantially purified amnion-derived cell populations, and to methods of creating such substantially purified amnion-derived cell populations, as well as methods of use. The invention is further directed to antibodies, in particular, monoclonal antibodies, that bind to amnion-derived cells or, alternatively, to one or more amnion-derived cell surface protein markers. The invention is further directed to methods for producing the antibodies, methods for using the antibodies, and kits comprising the antibodies. | 03-19-2009 |
| 20090104698 | Bioreactor System And Method For The Production And Collection Of Blood Cells From Engineered Bone Marrow Tissue - Methods and apparatus for a bioreactor system for growing cells are provided. The bioreactor system includes a reaction chamber for cultivating harvested tissue in a culture medium, and a hydrodynamic apparatus connected to the reaction chamber that generates a cyclic negative pressure differential in the bioreactor. Additionally, the apparatus includes a collection enclosure for collecting cells produced by tissue in the reaction chamber. Also encompassed are embodiments for harvesting and preparing cells and tissue sections, combining the cells and the tissue sections in a culture medium to cultivate tissue, maintaining the tissue under conditions that permit production of cell products, applying cyclic negative pressure to the culture over a period of time, and collecting cell products discharged. Further disclosed are embodiments for placing harvested tissue and cells in a diffusion chamber, creating an oscillating negative pressure differential in the bioreactor over a period of time, and collecting the cell products. | 04-23-2009 |
| 20090117653 | METHOD AND APPARATUS FOR EVALUATING THERAPEUTICS ON CANCER STEM CELLS - Embodiments provide cell culture methods to produce a 3-D model assembly that mimics in vitro the microenvironment of human bone marrow, where cells occupy distinct niches. Methods and apparatuses are provided for the efficient testing of cancer, including malignant hematopoietic cancers and metastatic spread to the bone marrow of solid tumors, and of other diseases of the blood and bone marrow. Methods and apparatuses are further provided in embodiments for the investigation of bone marrow cell biological characteristics and for the testing of therapeutics for nonmalignant diseases of the blood and bone marrow. | 05-07-2009 |
| 20090142834 | Multipotent stem cells from peripheral tissues and uses thereof - This invention relates to multipotent stem cells, purified from the peripheral tissue of mammals, and capable of differentiating into neural and non-neural cell types. These stem cells provide an accessible source for autologous transplantation into CNS, PNS, and other damaged tissues. | 06-04-2009 |
| 20090155907 | Removal of Embedding Medium - A method, apparatus and system for automated removal of an embedding medium from an embedded biological sample. The method comprising the steps of: providing an automated sample processing apparatus having an automated process operation capability that causes automated process operation events through robotic sample process functions; providing a clearing solvent, e.g. an organic solvent, capable of lowering the melting point of an embedding medium and/or dissolving an embedding medium; loading a plurality of carriers with embedded biological samples in the automated sample processing apparatus; exposing an embedded biological sample to the clearing solvent, whereby the embedding medium is liquefied; and providing a washing solution capable of removing the clearing solvent and the liquefied embedded medium from said biological sample, said clearing solvent and said washing solution being immiscible. | 06-18-2009 |
| 20090170199 | SEPARATION AND SORTING OF DIFFERENT BIOLOGICAL OBJECTS - The present invention relates to a method for the separation of biological objects in a solution which have different viscoelastic properties, wherein said method comprises a filtration step allowing the higher viscoelastic biological objects to pass through the membrane while retaining the lower viscoelastic biological objects above the membrane, and a recovery step wherein the separated lower viscoelastic biological objects are recovered above or onto the membrane and/or the separated higher viscoelastic biological objects are recovered in the filtrate. Advantageously, the biological objects are cells. More advantageously, the recovered cells are viable cells. In one preferred embodiment, the cells are tumor cells. In another preferred embodiment, the cells are fetal cells and the method finds an application in prenatal diagnosis. | 07-02-2009 |
| 20090221073 | DEVICES AND METHODS FOR CELL MANIPULATION - Devices for fluid control and biological particle manipulation (e.g., cell enrichment and blood sampling) are disclosed. The devices a based on the ability to control the flow of fluids through the use of microfluidic valves. The valves are characterized, for example, by microstructures disposed on a mobile diaphragm. | 09-03-2009 |
| 20090221074 | Materials and Procedures for the Purification of Cells - The subject invention provides new materials and methods for the efficient isolation and purification of stem cells. Specifically, conductive immunopolymers with stem cell specific antibodies can be used to remove stem cells from biological fluids. | 09-03-2009 |
| 20090317909 | SKELETAL MUSCLE PERIANGIOBLASTS AND CARDIAC MESANGIOBLASTS, METHOD FOR ISOLATION AND USES THEREOF - The present invention discloses the isolation and characterization of cells isolated either from adult skeletal muscle or from adult cardiac muscle. These cells are used for the treatment of muscular disorders including muscular dystrophy and cardiopathics, respectively. | 12-24-2009 |
| 20100015712 | SIDE POPULATION CELLS ORIGINATED FROM HUMAN AMNION AND THEIR USES - Cells which may be differentiated at least into nerve cells, which are useful for therapies of brain metabolic diseases, are disclosed. The cells are side population cell separated from human amniotic mesenchymal cell layer, in which expressions of Oct-4 gene, Sox-2 gene and Rex-1 gene are observed by RT-PCR, and which are vimentin-positive and CK19-positive in immunocytostaining. | 01-21-2010 |
| 20100087002 | Methods, Surface Modified Plates and Compositions for Cell Attachment, Cultivation and Detachment - The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity. | 04-08-2010 |
| 20100112694 | Dedifferentiation of Adult Mammalian Cardiomyocytes into Cardiac Stem Cells - Dedifferentiation is a mechanism whereby specialized cells regain properties of their ancestors, including, in the extreme, stemness. We found that highly-purified cardiomyocytes isolated from adult mammalian hearts dedifferentiated rapidly when cultured in mitogen-rich medium. Such myocytes reentered the cell cycle and proliferated, expressing stem cell surface markers such as c-kit and early cardiac transcription factors including GATA and NKx2.5. These myocyte-derived cells (MDC) were capable of re-differentiating into myocytes and endothelial cells. Contrary to prevailing dogma, cardiomyocyte dedifferentiation yields proliferative cells expressing stem cell markers and capable of multilineage differentiation. Cardiomyocyte dedifferentiation is a potential source of endogenous stem cells in the adult heart. | 05-06-2010 |
| 20100120148 | METHOD FOR DECOMPOSING BIOLOGICAL TISSUE - A biological tissue is decomposed in a short time, and cells are obtained at a high yield without interfering with the health of cells obtained through the decomposition. There is provided a method for decomposing biological tissue which decomposes a biological tissue under an action of a digestive enzyme, comprising: a decomposition starting step S | 05-13-2010 |
| 20100136689 | METHOD FOR DECOMPOSING BIOLOGICAL TISSUE - A biological tissue is decomposed in a short time, and cells are obtained at a high yield without interfering with the health of the cells obtained through the decomposition. There is provided a method for decomposing biological tissue which decomposes a biological tissue under an action of a digestive enzyme, wherein a digestive juice containing the digestive enzyme is additionally supplied with the digestive enzyme either continuously or stepwisely over a predetermined time within a period from start of the decomposition until termination of the decomposition while the biological tissue is being immersed in the digestive juice. | 06-03-2010 |
| 20100151574 | MULTIPOTENT PROGENITOR CELL DERIVED FROM ADIPOSE TISSUE - Disclosed is a cell mass containing an adipose-tissue-derived multipotent progenitor cell. Also disclosed is a method for producing an adipose-tissue-derived multipotent progenitor cell from an adipose tissue, which comprises the steps of: (a) removing erythrocytes from an adipose-tissue-derived cell mass to produce a preadipose-tissue-derived multipotent progenitor cell mass; and (b) removing cells other than the adipose-tissue-derived multipotent progenitor cell from the preadipose-tissue-derived multipotent progenitor cell mass to produce the desired adipose-tissue-derived multipotent progenitor cell. Further disclosed is an adipose-tissue-derived multipotent progenitor cell produced by the method. | 06-17-2010 |
| 20100285587 | Tissue Graft - A method of preparing a tissue graft material is disclosed. The disclosure also relates to a multipurpose tissue graft material and to methods of using same as a replacement for vascular and non-vascular tissue. | 11-11-2010 |
| 20100304487 | SOFT TISSUE PROCESSING - The present invention is a process for preparing soft tissue such as tendons, ligaments, cartilage, fascia, dermis, human valves and human veins for implant in a human and removes cellular components and forms an decellular matrix having as major components collagens and elastins while sterilizing the tissue. The process comprises the following steps: | 12-02-2010 |
| 20110070647 | PLURIPOTENT STEM CELL THAT CAN BE ISOLATED FROM BODY TISSUE - Objects of the present invention are to provide a method for directly obtaining pluripotent stem cells from body tissue and the thus obtained pluripotent stem cells. The present invention relates to SSEA-3 (+) pluripotent stem cells that can be isolated from body tissue. | 03-24-2011 |
| 20110104803 | Method for Collecting Functional Cells In Vivo with High Efficiency - Biologically low invasive vessels are filled with biological factors that have the activity of mobilizing specific functional cells in the body. The vessels are indwelled in the body. After specific functional cells are mobilized into the vessels, the vessels are removed from the body to collect functional cell populations mobilized to the vessels. Alternatively, the cells are directly collected from the vessels indwelled in the body. | 05-05-2011 |
| 20110117650 | Vessel and method for isolating cells from tissue portions - A vessel is adapted to be used in a procedure for isolating and collecting cells from a tissue portion using a centrifuge by providing a sealable chamber separated into two sections by a mesh screen, the chamber having a conical bottom inner surface, with a port at the apex in the lowest point in the chamber and ports at the top of the chamber, the method including placing the tissue portion in the chamber above the screen, washing the tissue, repeatedly as needed, by introducing a washing solution through a top port and draining the wash solution from the chamber through the bottom port, introducing an enzyme solution through a top port, allowing the enzyme solution to partially digest the tissue, centrifuging the vessel, and draining the isolated cells through the bottom port. | 05-19-2011 |
| 20110124105 | Mixed Cell Populations For Tissue Repair And Separation Technique For Cell Processing - The present invention provides a fluid exchange cell culture technique and tissue repair cells (TRCs) made by these methods, as well as methods using these cells. The method includes a new wash step which increases the tissue repair properties of the TRCs of the invention. This wash step allows for the production of TRC populations with greater tissue repair and anti-inflammatory capabilities. Embodiments of the present invention include a post-culture process for cultured cells that preferably includes the steps of: a wash process for removing unwanted residual culture components, a volume reduction process, and a harvesting process to remove cultured cells. Preferably, all these steps are performed within a aseptically closed cell culture chamber by implementing a separation method that minimizes mechanical disruption of the cells and is simple to automate. The harvested cells may then be concentrated to a final volume for the intended use. In such embodiments, the final composition is a substantially purified and concentrated cell mixture suspended in a physiologic solution suitable for immediate use in humans without further washing, volume reduction, or processing. Embodiments are also applicable to harvesting (and/or washing) particles within a liquid or solution within a chamber. | 05-26-2011 |
| 20110143438 | Process for Decellularizing Soft-Tissue Engineered Medical Implants, and Decellularized Soft-Tissue Medical Implants Produced - The invention provides methodologies and apparatus for producing a cellular soft-tissue implants, both in small quantities and in commercializable quantities. Such soft-tissue implants include vascular graft substitutes. An acellular graft is produced by subjecting the tissue sample to an induced pressure mediated flow of an extracting solution, followed by inducing a pressure mediated flow of a treating solution, then washing the treated tissue to produce the acellular graft. The acellular grafts produced are uniform and nonimmunogenic. | 06-16-2011 |
| 20110165676 | METHOD FOR DECELLULARIZATION - The present invention provides for decellularized tissue and method for decellularizing tissue. The method generally comprises the steps of obtaining a harvested tissue, performing a muscle shelf debridement, treating the tissue with an enzyme, washing the tissue with a detergent, and performing an organic solvent extraction on the tissue. The tissues decellularized according to the present invention have several advantages including removing more of the residual cell debris, dsDNA, and chemicals, as well as exhibiting less calcification and better ultimate tensile strength than tissues prepared not according to the method of the present invention. | 07-07-2011 |
| 20110250687 | Cell adhesion inhibitor (CAI) with combination growth factors mobilization of peripheral blood mononuclear cells for CAI derived dendritic cell (CdDC) preparation and dendritic cell vaccine preparations generated from CdDC - Disclosed is a method to recover a dendritic cell rich mixture from peripheral blood mononuclear cells (PBMC) mobilized with one or more cell adhesion inhibitors (CAI) for the preparation of a dendritic cell vaccine. The CAI derived dendritic cell rich mixture (CdDC) from PBMC can either be used alone or better still, be induced into dendritic vaccine specific preparations with the addition or modification with different antigens and methodologies of immunological induction methods known to the art. These CAI derived dendritic vaccines can then be used, but not exclusively so, in the treatment of cancer and infectious diseases. In order to achieve the best immature and mature dendritic cell rich mixture, peripheral blood cells mobilization may be achieved by administering, simultaneously or in sequence, to an individual one or more of a combination of different chemical compounds, hormones, growth factors etc. prior to PBMC collection with one or more of cell adhesion inhibitors such as a CXCR | 10-13-2011 |
| 20120100611 | PROCEDURE AND DEVICE FOR SEPARATING ADULT STEM CELLS FROM FATTY TISSUE - The invention relates to a method for separating adult stem cells from fatty tissue, wherein the fatty tissue is mixed with a fluid and the stem cells are subsequently separated. The fatty tissue with fat cells and adult stem cells is exposed to a fluid jet ( | 04-26-2012 |
| 20120156779 | METHOD FOR CELL EXPANSION - The present invention relates to a method for cell expansion. In the method, preferably a cell culture product is used, such as a microcarrier, or other adherent cell culture surface, comprising degradable polysaccharide, preferably starch, modified with small molecular weight cell-binding ligands. This allows recovery (detachment) of adhered cells to be aided by degradation of the culture surface with enzymatic agents, such as amylase. The method for cell expansion comprises the following steps: a) adding cells, culture medium and cell culture surface comprising a degradable polysaccharide with guanidine group containing ligands to a bioreactor; b) expanding said cells by adherent cell culture; and c) aiding the detachment of said cells by exposing them to a polysaccharidase to degrade the culturing surface. | 06-21-2012 |
| 20120178164 | TREATING CARDIOVASCULAR TISSUE - This document provides methods and materials for treating cardiovascular tissue. For example, stem cells, compositions containing stem cells, methods for obtaining stem cells, compositions for generating stem cells expressing particular markers, and methods for repairing cardiovascular tissue are provided. | 07-12-2012 |
| 20120220035 | TEMPERATURE-RESPONSIVE CO-POLYMERS AND USES THEREOF - This disclosure relates to temperature-responsive co-polymers, such as poly(N-isopropylacrylamide-co-hydroxypropyl methacrylate-co-3-trimethoxysilypropyl methacrylate). The disclosure also relates to polymeric films formed from these co-polymers and particular applications of these polymeric films, particularly in the cell culture field. More specifically, the present disclosure relates to the use of these polymeric films in the cell culture field to provide a means of controlling cellular adhesion to, and detachment from, the surface of a cell culture support substrate. | 08-30-2012 |
| 20120276628 | AUTOMATED SYSTEMS AND METHODS FOR ISOLATING REGENERATIVE CELLS FROM ADIPOSE TISSUE - A method of processing an adipose tissue to collect adipose derived regenerative cells is provided, wherein the method comprises providing a vessel comprising a fluid jet mixer; introducing the adipose tissue into the vessel; introducing a buffer solution into the vessel; washing the adipose tissue using the fluid jet mixer; introducing an enzyme solution into the vessel; initiating jet mixing into the vessel comprising the adipose tissue, the enzyme solution, and the buffer solution using the fluid jet mixer to digest the adipose tissue to form a digestion product; phase-separating the digestion product into a digested buoyant fat layer and a non-buoyant aqueous layer; and collecting the non-buoyant aqueous layer comprising the adipose derived regenerative cells. A system of processing an adipose tissue to collect adipose derived regenerative cells is also provided. | 11-01-2012 |
| 20120309089 | SUPPORTS FOR CELL CULTURE AND CELL SHEET DETACHMENT AND METHODS FOR CELL SHEET DETACHMENT - The invention creates a support for cell culture and cell sheet detachment which has a substrate, whose surface is coated with a conjugate having a disulfide bond-containing amino acid as a spacer and a biopolymer enhancing cell attachment, migration or differentation. Unexpectedly, after being seeded on the support, the cells grow to form one or more layers of cell sheets and the cell sheets can be easily detached from the support by adding a reductant to cleave the disulfide bond. Accordingly, the invention provides a simple and non-toxic method for detachment of cell sheets. | 12-06-2012 |
| 20130017606 | METHOD OF SEPARATING PANCREATIC ISLET - The present invention provides a pancreatic islet isolation method comprising the steps of (1) injecting a protection solution containing a protease inhibitor into the pancreatic duct of an procured pancreas; (3) digesting the pancreas into which the protection solution has been injected; and (4) purifying the digested pancreatic tissue using a purification solution containing a density gradient reagent. The present invention also provides a protection solution for injection into the pancreatic duct, a pancreas preservation solution for the two-layer method, and an islet purification solution. | 01-17-2013 |
| 20130109092 | Method for obtaining pancreatic endocrine cells from adipose tissue-origin cells | 05-02-2013 |
| 20130143323 | APPARATUS AND METHOD FOR DECELLULARIZING, RECELLULARIZING OR TREATING ORGANS - A biomedical device and method provide for decullularization, recellularization or other treatment of an organ of a human or animal. To keep pressures to a minimum and to ensure that the perfusion fluid uniformly perfused the organ, the organ is supported and rotated during the perfusion process. The organ is supported by a medium, which may comprise a liquid or pallets in a vessel, with a vessel mounted for limited rotation. The perfusion tubing, for supply of perfusion fluid to inform the organ can be mounted both to a support structure and the vessel. The perfusion system can include tubing for supply of air or an air substitute. | 06-06-2013 |
| 20130171729 | METHODS AND KITS FOR CELL RELEASE - Methods and kits of releasing cells are provided. The method comprises the steps of providing cultured cells on a cell culture support comprising a multi layer polyelectrolyte coating immobilized on a substrate, and releasing the cultured cells from the cell culture support by a releasing solution comprising DMSO. The kit comprises a cell culture support and a releasing solution. The releasing solution comprises DMSO. | 07-04-2013 |
| 20130252336 | Methods for Obtaining Target Cells - Methods for recovering target cells using degradable three dimensional matrices are described. | 09-26-2013 |
| 20140087465 | CELL CULTURE METHOD AND CELL CULTURE KIT - Provided is a means suitable for mass culture of adherent cells and which allows easy and safe collection of the cultured adherent cells. A cell culture kit has a culture bag having flexibility, such a level of hydrophilicity that the culture of adherent cells can be achieved, and permeability into a bag shape and which is filled with a culture medium; a dissociation solution bag filled with a dissociation solution containing a metal chelating agent or ornithine or a derivative thereof, and a solution sending circuit or a derivative thereof, and a solution sending circuit which connects the culture bag and the dissociation solution bag to each other and can send the dissociation solution from the dissociation solution bag to the culture bag. | 03-27-2014 |
| 20140315307 | MIXED POPULATION OF CELLS DERIVING FROM ADIPOSE TISSUE AND METHODS OF ISOLATING AND USING THE SAME - Adipose tissue-derived stromal cells and methods of isolating and using the same. In at least one embodiment of isolated adipose tissue-derived stromal cells of the present disclosure, the cells are isolated by performing adipose tissue resection or suction on a mammalian patient, dissecting tissue obtained from said tissue resection or suction and dissociating said tissue into a cell suspension, removing adipocytes from the cell suspension, culturing the adipocyte-depleted cell suspension in EGM-2-MV media, and isolating adipose tissue-derived stromal cells secreting vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and granulocyte-colony stimulating factor (G-CSF). | 10-23-2014 |
| 20140363891 | METHOD AND APPARATUS FOR PROCESSING ADIPOSE TISSUE - A portable apparatus for collection and processing of human biological material, such as containing adipose extracted during a lipoplasty procedure, is useful for multi-step processing to prepare a concentrated product (e.g., stromal vascular fraction) or a fat graft composition. The apparatus has a container with a containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a tapered portion to a collection volume for collecting concentrate product. Inlet and suction ports provide access to the tissue retention volume and filtrate volume, respectively, and an extraction port provides versatile access for removal of target processed concentrate material or fat graft material, which access may be via a lumen through a rotatable mixer shaft. Access ports may be configured for access only from above the container. A method of processing adipose tissue to concentrate leuko stromal vascular cells includes multi-step processing using a portable container. | 12-11-2014 |
| 20150024490 | CHEMICALLY LABILE PEPTIDE-PRESENTING SURFACES FOR CELLULAR SELF-ASSEMBLY - Methods of cell culture using patterned SAM arrays are disclosed. Advantageously, the disclosed methods use SAM arrays presenting adhesion peptides to grow confluent monolayers that can invaginate to form an embryoid body. | 01-22-2015 |
| 20150118751 | Tissue regenerative composition, method of making, and method of use thereof - A matrix, including epithelial basement membrane, for inducing repair of mammalian tissue defects and in vitro cell propagation derived from epithelial tissues of a warm-blooded vertebrate. | 04-30-2015 |
| 20150315540 | Presaturation of Supercritical CO2 with Water for Decellularization of Matrices - Decellularization methods for tissue are provided. The method can include: exposing a tissue to a water-saturated, supercritical CO | 11-05-2015 |
| 20150337263 | AUTOMATED METHODS FOR ISOLATING REGENERATIVE CELLS FROM ADIPOSE TISSUE - A method of processing an adipose tissue to collect adipose derived regenerative cells is provided, wherein the method comprises providing a vessel comprising a fluid jet mixer, introducing the adipose tissue into the vessel, introducing a buffer solution into the vessel; washing the adipose tissue using the fluid jet mixer; introducing an enzyme solution into the vessel; initiating jet mixing into the vessel comprising the adipose tissue, the enzyme solution, and the buffer solution using the fluid jet mixer to digest the adipose tissue to form a digestion product; phase-separating the digestion product into a digested buoyant fat layer and a non-buoyant aqueous layer; and collecting the non-buoyant aqueous layer comprising the adipose derived regenerative cells. A system of processing an adipose tissue to collect adipose derived regenerative cells is also provided. | 11-26-2015 |
| 20150344847 | Method For Production Of Large Numbers Of Cartilage Cells With Phenotype Retention - A method for production of large numbers of cartilage cells with phenotype retention intended for treatment of articular cartilage lesions or preparing viable cartilage tissue by propagation of chondrocytes from cartilage explants, human or animal, with the retention of phenotypes of the cartilage cells, in which cells are chondrocytes retaining morphologic attributes of the same or chondrocyte progenitor cells. The method includes culturing the cells in which cultured cells are organized into mature hyaline cartilage on the surface of biologic structures such as cancellous or cortical bone lamina. The method for culturing chondrocytes to produce three dimensional cellular structures consisting of cartilage cells and cell produced extracellular cartilage matrix. | 12-03-2015 |
| 20150368603 | TISSUE PROCESSING APPARATUS WITH FILTER PIERCEABLE TO REMOVE PRODUCT AND METHOD FOR PROCESSING ADIPOSE TISSUE - A portable apparatus for collection and processing of human biological material, such as containing adipose extracted during a lipoplasty procedure, is useful for multi-step processing to prepare a concentrated product (e.g., stromal vascular fraction). The apparatus has a container with a containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a tapered portion to a collection volume for collecting concentrate product. Inlet and suction ports provide access to the tissue retention volume and filtrate volume, respectively, and an extraction port provides versatile access for removal of target processed concentrate material. Advancing a hollow member such as a hypodermic needle through the extraction port and into the collection volume pierces the filter with the hollow member, providing a safety feature to prevent reuse of the apparatus. Access ports may be configured for access only from above the container. A method of processing adipose tissue to concentrate stromal vascular cells includes multi-step processing using a portable container. | 12-24-2015 |
| 20160017272 | Devices and Methods for Purification, Detection and Use of Biological Cells - This invention relates to devices and methods for purifying, detecting and using biological cells. A variety of cell types including viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a column, including a pipette tip column. Methods of the invention can aid research, diagnosis and treatment of cancer. Purified viable cells can be detected on the column or eluted from the column and detected. Cells on a column can be used as a stationary phase for liquid chromatography. Cells may be removed, recovered and analyzed. | 01-21-2016 |
| 20160122704 | METHOD FOR SEPARATION OF SPORADIC CELLS FROM BODY FLUIDS, AND APPARATUS FOR CARRYING OUT SAID METHOD - Method for gentle separation of viable sporadic cells from body fluids such as blood, from malignant effusions, bronchoalveolar lavage fluid, peritoneal lavage fluid and amniotic fluid, based on a filter membrane which is in an intimate contact with an absorbent material. Using the present method it is possible to isolate for example circulating and disseminated tumor cells, endometrial cells and circulating trophoblast cells, allowing subsequent detection, quantification, characterization and especially culturing of said cells. An apparatus for carrying out the method is further disclosed. | 05-05-2016 |
| 20160123848 | SEMI-AUTOMATED SAMPLING SYSTEM FOR ASEPTIC SAMPLING - A semi-automated sampling assembly configured for aseptic sampling at one or more instances from a sample source having a biological inoculum is provided. The semi-automated sampling assembly includes a sampling conduit, a recovery conduit, one or more sampling kits, and a pumping device. The sampling conduit includes a first port and a second port, where the first port of the sampling conduit is configured to be operatively coupled to the sample source. Further, the recovery conduit includes a first port and a second port, where the first port of the recovery conduit is configured to be operatively coupled to the sample source. Also, the second port of the recovery conduit is operatively coupled to at least a portion of the sampling conduit. Moreover, the one or more sampling kits are operatively coupled to the sampling conduit, and the pumping device is operatively coupled to the sampling conduit. | 05-05-2016 |
| 20160168531 | RECOVERY AND PURITY OF MAGNETICALLY TARGETED CELLS | 06-16-2016 |
| 20160186132 | PROCESS FOR PRODUCING MULTIPOTENT STEM CELLS AND PROGENITORS - The present invention relates to a non-enzymatic process for producing multipotent stem cells and progenitors from the cultivation of stem cell niches. | 06-30-2016 |
| 20160376551 | METHOD OF MAKING BIOLOGICAL TISSUE COMPONENTS - A method of preparing a biological composite matrix system includes combining tissue microparticles with a curable medium. The tissue microparticles are formed by pulverizing tissue, sorting the pulverized tissue particles, and decellularizing the pulverized tissue particles. The tissue microparticles are then mixed with the curable medium and the resulting composite is cured to form a microparticle-medium composite matrix. The microparticle-medium composite matrix is compressed to increase the strength of the matrix and to increase the density of the microparticles to a predetermined density. | 12-29-2016 |
| 20190144813 | PARTICLES FOR USE IN ACOUSTIC STANDING WAVE PROCESSES | 05-16-2019 |
