Entries |
Document | Title | Date |
20080227170 | Use of Pit Emulsions in Biocatalytic Reactions - Oil-in-water emulsions produced by the phase inversion temperature process, with droplet sizes of 50 to 400 nm, for use as the reaction medium for biocatalytic reactions. | 09-18-2008 |
20080248546 | Mutant 2,5-diketo-L-gluconic acid reductases - Described herein are novel nucleic acids, proteins and methods that can be used to provide new catalysts with desirable traits for industrial processes. In particular, novel reductases isolated from the environment using PCR methods are described. | 10-09-2008 |
20080268517 | STABLE, FUNCTIONAL CHIMERIC CYTOCHROME P450 HOLOENZYMES - The present disclosure relates to cytochrome p450 fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides. | 10-30-2008 |
20080318295 | Ketoreductases and Uses Thereof - The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. | 12-25-2008 |
20090029437 | NOVEL GLUCOSE DEHYDROGENASE AND METHOD FOR PRODUCING THE DEHYDROGENASE - A novel glucose dehydrogenase, which is an enzyme that has high substrate specificity, can be produced at a low cost, is not affected by oxygen dissolved in a measurement sample and, in particular, has superior thermal stability is obtained by culturing a microorganism belonging to the genus | 01-29-2009 |
20090029438 | COMPOSITE MATERIALS COMPRISING SUPPORTED POROUS GELS - This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth. | 01-29-2009 |
20090042269 | Isolation of quiescin-sulfhydryl oxidase from milk - Methods for isolating a highly purified QSOX enzyme from milk or whey are described. The enzyme may be used to generate intermolecular and intramolecular disulfide bridges and to facilitate oxidative protein folding. | 02-12-2009 |
20090081760 | DMO METHODS AND COMPOSITIONS - The invention provides for identification and use of crystal structures of Dicamba monooxygenase (DMO) that may be complexed with iron or cobalt cofactor and substrate (dicamba), or product (DCSA) in order to define residues important for enzymatic structure and function. Methods of using such structures are described. Data storage media comprising the crystal structural coordinate information are also described. | 03-26-2009 |
20090087893 | Modified Expandase Enzyme and its use - The present invention relates to a mutant penicillin expandase which comprises an amino acid substitution at one or more residue positions corresponding to those of a wild-type expandase selected from the group consisting of threonine at position 42, isoleucine at position 50, histidine at position 57, threonine at position 67, valine at position 133, threonine at position 143, proline at position 145, glycine at position 148, phenyl alanine at position 152, proline at position 196, alanine at position 240, cysteine at position 281, Serine at position 309, provided that the amino acid substitution at the residue position of cysteine at position 281 is not tyrosine. | 04-02-2009 |
20090098630 | FUSION PROTEIN, GENE RELATED TO FUSION PROTEIN, VECTOR, TRANSFORMANTS, AND ANTI-INFLAMMATORY MEDICINAL COMPOSITION - Fusion protein comprises a thioredoxin and human serum albumin. The thioredoxin comprises a protein comprising the amino acid sequence shown in SEQ. ID. No. 1, or the like, the human serum albumin comprises a protein comprising the amino acid sequence shown in SEQ. ID. No. 2, or the like. The fusion protein of the invention allows the activity of thioredoxin to be maintained over a long period of time. It is thus possible to provide a protein preparation that is stable over long periods of time. | 04-16-2009 |
20090117638 | BILIRUBIN OXIDASE MUTANT HAVING THERMAL STABILITY - A heat-resistant bilirubin oxidase mutant is disclosed. The bilirubin oxidase is obtained by deletion, replacement, addition or insertion of at least one amino acid residue of a wild type amino sequence of SEQ. ID. No. 1 of a bilirubin oxidase derived from an imperfect filamentous fungus, | 05-07-2009 |
20090117639 | Neural Progenitor Cell Populations - This invention provides populations of neural progenitor cells, differentiated neurons, glial cells, and astrocytes. The populations are obtained by culturing stem cell populations (such as embryonic stem cells) in a cocktail of growth conditions that initiates differentiation, and establishes the neural progenitor population. The progenitors can be further differentiated in culture into a variety of different neural phenotypes, including dopaminergic neurons. The differentiated cell populations or the neural progenitors can be generated in large quantities for use in drug screening and the treatment of neurological disorders. | 05-07-2009 |
20090142821 | Peroxide-driven cytochrome P450 oxygenase variants - The invention relates to novel variants of cytochrome P450 oxygenases. These variants have an improved ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. These variants also have an improved thermostability as compared to the cytochrome P450 BM-3 F87A mutant. Preferred variants include cytochrome P450 BM-3 heme domain mutants having 158V, F87A, H100R, F107L, A135S, M145A/V, N239H, S274T, L3241, I366V, K434E, E442K, and/or V446I amino acid substitutions. | 06-04-2009 |
20090155880 | MUTANT PROTEIN HAVING DIAPHORASE ACTIVITY - A mutant protein having diaphorase activity is provided. A mutant protein includes an amino acid sequence obtained by deletion, replacement, addition, or insertion of at least one amino acid residue of a native-form amino acid sequence of SEQ. ID. No. 1, wherein the mutant protein has diaphorase activity with an enzyme activity of 245 or more. | 06-18-2009 |
20090269826 | VACCINE AGAINST STREPTOCOCCUS AGALACTIAE INFECTION USING NATIVE OR RECOMBINANT S. AGALACTIAE GLYCERALDHEYDE-3-PHOSPHATE DEHYDROGENASE (GAPDH) AS A TARGET ANTIGEN - The present invention regards a vaccine against | 10-29-2009 |
20090269827 | Thermophilic and thermoacidophilic metabolism genes and enzymes from alicyclobacillus acidocaldadarius and related organisms, methods - Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from | 10-29-2009 |
20090275102 | EHRLICHIA DISULFIDE BOND FORMATION PROTEINS AND USES THEREOF - Novel genes encoding homologous immunoreactive thio-disulfide oxidoreductases, or disulfide bond formation (Dsb) proteins from | 11-05-2009 |
20090311768 | Thermostable superoxide dismutase - The present invention provides a thermostable superoxide dismutase from a thermophile. The thermostable superoxide dismutase that is able to catalyze the conversion of superoxide ions or radicals under the moderate to high temperature environments. | 12-17-2009 |
20090311769 | Thermostable luciferases and methods of production - Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits. | 12-17-2009 |
20090325264 | Engineered Delta-15-Fatty Acid Desaturases - The present invention provides engineered fatty acid desaturase molecules preferring Gamma Linolenic Acid (GLA) over Linoleic Acid (LA) as a substrate. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds comprising the desaturase molecule, and methods for preparing and using the same. In particular, the disclosed engineered desaturase molecules are capable of altering the omega-3 fatty acid profiles in plants and plant parts. | 12-31-2009 |
20100028972 | KETOREDUCTASE POLYPEPTIDES AND RELATED POLYNUCLEOTIDES - The present invention is directed to variant polypeptides having enhanced ketoreductase activity and/or thermostability for use in the stereospecific reduction of ketones. In addition, the present invention is directed to polynucleotides that encode the ketoreductase polypeptides, including codon optimized versions of the polynucleotides which provide for enhanced expression in host cells. In another aspect, the present invention is directed to nucleotide constructs, vectors and host cells that are transformed with polynucleotides of the present invention. | 02-04-2010 |
20100129891 | BILIRUBIN OXIDASE MUTANT HAVING THERMAL STABILITY - A heat-resistant bilirubin oxidase mutant is disclosed. The bilirubin oxidase mutant is obtained by deletion, replacement, addition or insertion of at least one amino acid residue of a wild type amino sequence of SEQ. ID. No. 1 of a bilirubin oxidase derived from an imperfect filamentous fungus, | 05-27-2010 |
20100144009 | Extracting And Purifying Lipoxygenase - The present invention relates to the extraction and purification of lipoxygenase from plant matter, including soy beans. A process for purifying lipoxygenase from plant matter is provided. | 06-10-2010 |
20100151554 | ENZYME COMPOSITION AND USE THEREFORE - The present invention provides an enzyme composition and a method for treating coal using the enzyme composition. The enzyme composition includes at least one enzyme, coenzyme and ammonium acetate, wherein the enzyme can be laccase-isozyme, pyruvate dehydrogenase, dihydrolipoyl transacetylase, and dihydrolipoyl dehydrogenase, and the coenzyme can be CoA, CoA-SH, thiamine pyrophosphate, lipoic acid, flavin adenine dinucleotide, and nicotinamide adenine dinucleotide. In addition, the method of the present invention includes treating the coal with the enzyme composition for more than 72 hours. | 06-17-2010 |
20100184185 | Isolated luciferases and the use thereof - The invention relates to the nucleotide and amino acid sequences, and to the activity and use, of the luciferases LuAL, Lu164, Lu16, Lu39, Lu45, Lu52 and Lu22. | 07-22-2010 |
20100184186 | NOVEL RIGIDOPORUS MICROPORUS LACCASE - An isolated | 07-22-2010 |
20100203614 | FATTY ALDEHYDE REDUCTASE - This disclosure relates to the polynucleotide sequences of SEQ ID NO: 1 and SEQ ID NO: 4, and to the amino acid sequences of SEQ ID NO: 2 and SEQ ID NO: 3, and to novel fatty aldehyde reductase enzymes provided by the same. | 08-12-2010 |
20100233780 | Methods for Improving Protein Properties - The present invention provides methods for engineering proteins to optimize their performance under certain environmental conditions of interest. In some embodiments, the present invention provides methods for engineering enzymes to optimize their catalytic activity under particular environmental conditions. In some preferred embodiments, the present invention provides methods for engineering enzymes to optimize their catalytic activity and/or stability under adverse environmental conditions. In some preferred embodiments, the present invention provides methods for engineering enzymes to optimize their storage stability, particularly under adverse environmental conditions. In some preferred embodiments, the present invention provides methods for altering the net surface charge and/or surface charge distribution of enzymes (e.g., metalloproteases) to obtain enzyme variants that demonstrate improved performance and/or stability in detergent formulations as compared to the starting or parent enzyme. | 09-16-2010 |
20100240113 | METHOD OF DEGUMMING JUTE FIBRES WITH COMPLEX ENZYME (1) - A method of degumming jute fibres with complex enzyme, wherein said complex enzyme comprises pectinase and laccase, wherein comprising the steps of: a. soaking the jute fibres in the water solution of said complex enzyme made from pectinase and laccase, wherein the weight proportion of said complex enzyme water solution and jute fibres ranges from 12:1 to 40:1; b. adjusting the PH value of said complex enzyme water solution to more than 5.0, but no more than 6.5, and adjusting the temperature of said complex enzyme water solution to 35° C.-65° C., then keeping said complex enzyme water solution with such temperature value for 20-120 minutes; c. adjusting the PH value of said complex enzyme water solution to 7.5-9.5, and adjusting the temperature of said complex enzyme water solution to 40° C.-70° C.; then, keeping said complex enzyme water solution with such temperature value for 20-120 minutes; d. conducting enzyme deactivation of the jute fibres processed with said complex enzyme. | 09-23-2010 |
20100248327 | CYTOCHROME P450 OXYGENASES - Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3. | 09-30-2010 |
20100261249 | RECOMBINANT C-TERMINAL ALPHA-AMIDATING ENZYME DERIVATIVE - Disclosed are: a recombinant C-terminal α-amidated enzyme derivative which lacks the formation of at least one disulfide bond among five disulfide bonds occurring in a C-terminal α-amidated enzyme derived from | 10-14-2010 |
20100267110 | LACCASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM - The invention provides laccases, polynucleotides encoding these enzymes, the methods of making and using these polynucleotides and polypeptides. | 10-21-2010 |
20100285565 | ESCHERICHIA COLI EXPRESSING THE CYTOCHROME P-450 GENE AND A METHOD FOR MICROBIAL CONVERSION USING THEM - Disclosed is a means for improving the poor conversion efficiency in a bioconversion system using an | 11-11-2010 |
20110003360 | DELTA-6 DESATURASE AND USES THEREOF - The subject invention relates to the identification of genes involved in the desaturation of polyunsaturated fatty acids at carbon 5 (i.e., “Δ5-desaturase”) and at carbon 6 (i.e., “Δ6-desaturase”) and to uses thereof. In particular, Δ5-desaturase may be utilized, for example, in the conversion of dihomo-γ-linolenic acid (DGLA) to arachidonic acid (AA) and in the conversion of 20:4n-3 to eicosapentaenoic acid (EPA). Delta-6 desaturase may be used, for example, in the conversion of linoleic (LA) to γ-linolenic acid (GLA). AA or polyunsaturated fatty acids produced therefrom may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics. | 01-06-2011 |
20110008867 | COMPOSITIONS AND METHODS FOR THE PRODUCTION OF A COMPOUND - The invention features compositions and methods that are useful for the production of a compound. | 01-13-2011 |
20110008868 | MULTIZYMES - Multizymes (i.e., single polypeptides having at least two independent and separable enzymatic activities) are disclosed for a variety of applications beyond the synthesis of long chain polyunsaturated fatty acids. | 01-13-2011 |
20110014677 | NOVEL DNA FRAGMENT, RECOMBINANT VECTOR COMPRISING SAME, TRANSFORMANT TRANSFORMED THEREWITH, AND USE THEREOF - A DNA fragment containing a gene which encodes a specific gene regulatory region alone or the gene regulatory region together with a signal peptide; a recombinant vector containing the DNA fragment; a transformant containing the recombinant vector; and a method of producing a recombinant protein by using the transformant. According to the invention, it is possible to produce a protein in a large amount at a high efficiency regardless of the kind of the recombinant protein. | 01-20-2011 |
20110059503 | Compositions of variant biocatalysts for preparing enantiopure amino acids - A composition of variant biocatalysts, specifically variants of | 03-10-2011 |
20110076739 | CATALYTIC DOMAINS FROM LYSYL OXIDASE AND LOXL2 - Disclosed herein are amino acid sequences, and encoding nucleotide sequences, of isolated catalytic domains of the LOX and LOXL2 proteins from human and mouse. Methods for the preparation and use of these isolated catalytic domains are also provided. | 03-31-2011 |
20110129895 | Method For Purifying Protein - A method is provided for purifying protein having steps of loading a sample containing proteins into a column containing an ion exchanger in a first direction to allow the ion exchanger to absorb proteins; and passing a salt solution through the column in a second direction opposite to the first direction to elute a target protein from the ion exchanger. The method is proven as capable of improving homogeneity of eluted target protein without combining minute and complicated techniques for protein purification. Therefore, the method is convenient, efficient and economic for purifying proteins. | 06-02-2011 |
20110189755 | METHOD FOR THE PRODUCTION OF AN ADDITIVE FOR THE ENZYMATIC DECOMPOSITION OF MYCOTOXINS, ADDITIVE, AND USE THEREOF - In a method for producing an additive for the enzymatic degradation of mycotoxins, in particular fumonisins, it is provided that at least one nucleic acid sequence of genes corresponding to sequences ID Nos. 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24 is provided, the at least one nucleic acid sequence is expressed in prokaryotic or eukaryotic host cells, and at least one thus prepared enzyme corresponding to sequences ID Nos. 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23 and 25, or at least one complete recombinant host organism optionally along with a cosubstrate, are used in a vegetable raw material. | 08-04-2011 |
20110201081 | Buttiauxella Sp. Phytase Variants - Provided herein are variants of | 08-18-2011 |
20110212502 | [NiFe]-Hydrogenases Having an Improved Resistance to Dioxygen, Process for Obtaining Them and Their Applications - The invention relates to [NiFe]-hydrogenases having an improved resistance to dioxygen, said [NiFe]-hydrogenases may be obtained by:—providing an initial polynucleotide comprising a sequence encoding a large subunit of a [NiFe]-hydrogenase, said large subunit comprising the following peptide motifs: L1: RGXE, wherein X=L, I, F, V or M L2: [R/K]X | 09-01-2011 |
20110212503 | METHOD FOR DESIGNING HEAT-RESISTANT TYROSINE-DEPENDENT SHORT-CHAIN DEHYDROGENASE/REDUCTASE AND HEAT-RESISTANT TYROSINE-DEPENDENT SHORT-CHAIN DEHYDROGENASE/REDUCTASE - A method for designing a heat-resistant mutant enzyme, the method including the step of reducing a distance between the α4 helix and the α6 helix in a protein three-dimensional structure to become smaller than that of a wild type enzyme through deletion, replacement, addition, or insertion of one or several amino acids in the amino acid sequence of the wild type enzyme with respect to tyrosine-dependent short-chain dehydrogenase/reductase. | 09-01-2011 |
20110217754 | KETOREDUCTASE POLYPEPTIDES FOR THE PRODUCTION OF (R)-3-HYDROXYTHIOLANE - The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds. | 09-08-2011 |
20110229955 | LUCIFERASES AND METHODS FOR MAKING AND USING THE SAME - Briefly described, embodiments of this disclosure include polynucleotides that encode mutant | 09-22-2011 |
20110275135 | Genetic elements, proteins, and associated methods including application of additional genetic information to gram (+) thermoacidophiles - Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from | 11-10-2011 |
20110281323 | Novel Fungal Laccases and Uses Thereof - Novel laccases from | 11-17-2011 |
20110294190 | MUTANT HUMAN SUPEROXIDE DISMUTASE 1 VARIANTS - The present invention in the art of biochemistry claims novel and non-naturally occurring engineered mutant “human superoxide dismutase 1” (hsod1) variant polypeptides, their encoding nucleic acids, and recombinant cells thereof. The inventor rationally engineered mutant hsod1 variants using structural observations and complimentary experimentation. The engineered mutant hsod1 variant products claimed have multiple potential industrial applications, including as novel therapeutics. | 12-01-2011 |
20120009647 | THERMOSTABLE LUCIFERASES AND METHODS OF PRODUCTION - Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits. | 01-12-2012 |
20120015420 | DELTA-8 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY ACIDS - Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding a delta-8 desaturase along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using this delta-8 desaturase in plants and oleaginous yeast are disclosed. | 01-19-2012 |
20120028333 | USE OF ENZYMES TO REDUCE ALDEHYDES FROM ALDEHYDE-CONTAINING PRODUCTS - The invention relates to the use of an enzyme preparation which catalyzes the degradation of formaldehyde for reducing the formaldehyde content in a formaldehyde-containing formulation. In a preferred embodiment, the enzyme preparation contains a formaldehyde dismutase from a | 02-02-2012 |
20120034672 | SUPERLUMINESCENT LUCIFERASE VARIANT WITH PROLONGED BIOLUMINESCENCE - This invention provides a genetically modified marine luciferase such as | 02-09-2012 |
20120064601 | Chromatography Membranes for the Purification of Chiral Compounds - Described herein are composite materials and methods of using them for the separation or purification of enantiomers. In certain embodiments, the composite material comprises a support member, comprising a plurality of pores extending through the support member; and a macroporous cross-linked gel, comprising a plurality of macropores, and a plurality of pendant chiral moieties. In certain embodiments, the composite materials may be used in the separation or purification of a chiral small molecule. | 03-15-2012 |
20120107904 | CARBOXY TERMINAL RESIDUES AS PREDICTORS AND REGULATORS OF PROTEIN STABILITY - Compositions and methods are provided that are useful for predicting and controlling the stability of expressed polypeptides. The compositions and methods may be used to predict and as desired, increase or decrease the stability of proteins recombinantly expressed in mycobacteria, for example DesA3 expressed in | 05-03-2012 |
20120107905 | Variants of Fungal Serine Protease - The present invention relates to fungal serine protease variants, which comprise an amino acid substitution of valine at position 208 of the parent | 05-03-2012 |
20120115203 | METHOD FOR PRODUCING 4-HYDROXY-L-ISOLEUCINE - A highly active L-isoleucine dioxygenase from | 05-10-2012 |
20120122182 | GAUSSIA LUCIFERASE VARIANT FOR HIGH-THROUGHPUT SCREENING - Described herein is a variant of wild type Gaussia luciferase that catalyzes glow-type emission kinetics suited for high-throughput functional screening applications. Polypeptides, functional fragments, variants, and nucleic acids that encode the enhanced luciferase are further described. One such polypeptide corresponds to wild type Gaussia luciferase with a substitution mutation of I for M at position 43 of the mature peptide. Methods of use, assay systems and kits that contain the polypeptides and/or nucleic acids are further described. | 05-17-2012 |
20120178141 | LUCIFERASES AND METHODS FOR MAKING AND USING THE SAME - Briefly described, embodiments of this disclosure include polynucleotides that encode mutant | 07-12-2012 |
20120184015 | CYTOCHROME P450 OXYGENASES - Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3. | 07-19-2012 |
20120196347 | DELTA-8 DESATURASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS - Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) and using these delta-8 desaturases in plants. | 08-02-2012 |
20120220010 | PHOTOSYNTHETIC HYDROGEN PRODUCTION FROM THE GREEN ALGA CHLAMYDOMONAS REINHARDII - The present invention relates generally to hydrogen production for use in fuel cells, foodstuffs and chemical production, and more particularly, to biologically and photosynthetically produced hydrogen. Specifically, disclosed is a method for producing bacteria and green alga that can produce hydrogen in quantities that exceed four hundred percent of the hydrogen produced by green alga in nature; thus, producing organisms which can serve as hydrogen generators for fuel cells, chemical production and numerous other applications. | 08-30-2012 |
20120276610 | Isolation of A Protein Responsible For Uranium (VI) Reduction - The present invention relates to the isolation and characterization of a protein responsible for the reduction of uranium (VI) to uranium (IV). The present invention extends to the use of the isolated protein in the reduction of uranium (VI) to uranium (IV) and further extends to a process for the bioremediation, or at least partial remediation, of a site contaminated with a source of U (VI). According to a first aspect thereof, the present invention provides an isolated polypeptide derived from | 11-01-2012 |
20120295324 | OVERPRODUCTION OF LIGNINOLYTIC ENZYMES - Methods, compositions, and systems for overproducing ligninolytic enzymes from the basidiomycetous fungus are described herein. As described, the method can include incubating a fungal strain of | 11-22-2012 |
20120295325 | FIREFLY LUCIFERASE, ITS GENE, AND METHOD FOR PRODUCING FIREFLY LUCIFERASE - A firefly luciferase having the amino acid sequence of firefly luciferase, wherein the amino acid corresponding to position 287 of Heike firefly luciferase is replaced with alanine, or wherein the amino acid corresponding to position 392 is replaced with isoleucine. The firefly luciferase has improved thermostability and storage stability. A firefly luciferase gene coding for the firefly luciferase. By utilizing this gene, it is possible to efficiently produce firefly luciferase with increased stability. It is also possible to obtain firefly luciferase with further increased stability by combination with a mutation in which the amino acid at position 326 is replaced with serine, and/or a mutation in which the amino acid at position 467 is replaced with isoleucine. | 11-22-2012 |
20120295326 | LUCIFERASES AND METHODS FOR MAKING AND USING THE SAME - Briefly described, embodiments of this disclosure include polynucleotides that encode mutant | 11-22-2012 |
20120322136 | POLYNUCLEOTIDES ENCODING RECOMBINANT KETOREDUCTASE POLYPEPTIDES - The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl(1,3-oxazolidin-3-yl))-1-(4-fluorophenyl)pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe. | 12-20-2012 |
20120329131 | METHOD FOR INCREASING THE ACTIVITY OF SUPEROXIDE DISMUTASE - A method for increasing the activity of superoxide dismutase comprises steps of: liquid culture, by providing a strain of | 12-27-2012 |
20130011902 | STIMULATION OF THE SYNTHESIS OF THE ACTIVITY OF AN ISOFORM OF LYSYL OXIDASE-LIKE LOXL FOR STIMULATING THE FORMATION OF ELASTIC FIBERS - The invention relates to the stimulation of the synthesis and of the activity of an isoform of lysyl oxidase, and more particularly of the LOXL (lysyl oxidase-like) isoform. The invention relates notably to a method of identifying an active principle which stimulates the formation of elastic fibers. The aim of the invention is mainly to provide such a method of identification so as to provide compositions which enable stimulating the formation of elastic fibers. | 01-10-2013 |
20130029400 | GENETIC ELEMENTS, PROTEINS, AND ASSOCIATED METHODS INCLUDING APPLICATION OF ADDIITNAL GENETIC INFORMATION TO GRAM (+) THERMOACIDOPHILES - Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from | 01-31-2013 |
20130034894 | NOVEL ALLENE OXIDE SYNTHASE DERIVED FROM LEMNA PAUCICOSTATA - A highly active allene oxide synthase that can be used in the production of a plant growth regulating agent (KODA) is provided. | 02-07-2013 |
20130040364 | POLYNUCLEOTIDES ENCODING KETOREDUCTASES FOR PRODUCING STEREOISOMERICALLY PURE STATINS AND SYNTHETIC INTERMEDIATES THEREFOR - The present disclosure provides ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, method of using the engineered ketoreductase enzymes to synthesize a variety of chirally pure compounds, and the chirally pure compounds prepared therewith. | 02-14-2013 |
20130052714 | RECOMBINANT BILIRUBIN OXIDASE AND PRODUCTION METHOD FOR THE SAME - Disclosed is a method of producing a recombinant bilirubin oxidase, including a step wherein a bilirubin oxidase gene derived from imperfect filamentous fungus | 02-28-2013 |
20130095550 | POLYNUCLEOTIDES HAVING PROMOTER ACTIVITY - The present invention relates to isolated polynucleotides having promoter activity the use of the isolated polynucleotides for the production of a polypeptide. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing a desired polypeptide using the polypeptide having promoter activity. | 04-18-2013 |
20130203149 | COLOR REACTION USING TYROSINASE AND PEPTIDE - The problem to be solved by the present invention is to provide a method and a kit for easily performing a color reaction for coloration of bright green to blue colors by using tyrosinase. The above-mentioned problem was solved by providing a coloration method comprising the steps of: (a) mixing peptide and tyrosinase; (b) incubating the mixture; and (d) cryopreserving the incubated mixture; and by providing a kit for performing a color reaction, comprising: (i) peptide; and (ii) tyrosinase. | 08-08-2013 |
20130224829 | NOVEL LIPOXYGENASE DERIVED FROM LEMNA PAUCICOSTATA - A highly active novely lypoxygenase cDNA derived from Lemna paucicostata SH strain and Lemna paucicostata 441 strain and a protein encoded by the cDNA are provided. | 08-29-2013 |
20130224830 | EGGSHELL MEMBRANE SOLUBILIZATION METHOD USING ENZYMES - The present invention addresses the problem of providing an eggshell membrane solubilization method that is capable of solving the problems associated with carrying out treatment using acids and alkalis, or problems associated with the processing methods of the conventional art that use proteases; in other words, an eggshell membrane solubilization method that is capable of solving at least one of the following problems: (1) the need for pretreatment such as pulverization, sonication or boiling; (2) the need for prolonged treatment; and (3) a low decomposition rate (approximately 20%). Eggshell membranes are efficiently solubilized by using a protease in combination with a reducing agent. | 08-29-2013 |
20130252307 | ANCESTRAL PROTEINS - The invention provides a method for increasing the stability and/or activity of a polypeptide at low pH and/or elevated temperatures. The invention further provides a method for increasing the melting temperature of a polypeptide. Also provided are paleoenzymologically reconstructed thioredoxin polypeptides having activity at higher temperatures and/or lower pH than extant thioredoxin polypepetides, as well as paleoenzymologically reconstructed thioredoxin polypeptides having higher melting temperatures than extant thioredoxin polypepetides. | 09-26-2013 |
20130273631 | Novel Fungal Laccases and Uses Thereof - Novel laccases from | 10-17-2013 |
20130280784 | ESCHERICHIA COLI EXPRESSION SYSTEM FOR PRODUCING MATURE HUMAN TYROSINASE AND A PRODUCING METHOD THEREOF | 10-24-2013 |
20130309749 | NUCLEIC ACID ENCODING N-METHYLPUTRESCINE OXIDASE AND USES THEREOF - The gene encoding N-methylputrescine oxidase (MPO) and constructs comprising such DNA are provided, including methods of regulating MPO expression independently or with other alkaloid biosynthesis genes to modulate alkaloid production in plants and host cells. MPO genes or fragments thereof are useful for reducing pyrrolidine or tropane alkaloid production in plants, for increasing pyrrolidine or tropane alkaloid production in plants, and for producing an MPO enzyme in host cells. | 11-21-2013 |
20130323814 | METHOD FOR HIGHLY SENSITIVE DETECTION OF PROTEIN-PROTEIN INTERACTION - The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex. | 12-05-2013 |
20140065691 | Delta-9 desaturase used in the biosynthesis of polyunsaturated fatty acids - The present invention provides novel fatty acid desaturases genes used for synthesis of polyunsaturated fatty acids, especially delta-9 desaturases (FADS9-I). The present invention also provides nucleic acid sequence coding the above-described desaturases, expression vector of the above-described desaturases and recombinant microorganism expressing above-described desaturases. | 03-06-2014 |
20140073031 | STAR-WORM LUCIFERASE - The present invention relates to a luciferase derived from a star-worm belonging to genus | 03-13-2014 |
20140080199 | LUCIFERASE DERIVED FROM LUCIDINA ACCENSA - An object of the invention is to provide a novel and useful luciferase. The luciferase according to the embodiments of the invention is derived from | 03-20-2014 |
20140186918 | LUCIFERASE MUTANT - A recombinant protein having luciferase activity and at least 60% similarity to a wild-type luciferase wherein in the sequence of the enzyme, the amino acid residue corresponding to residue 357 in | 07-03-2014 |
20140273156 | LUCIFERASE BIOSENSOR - A modified beetle luciferase protein which is an environmentally sensitive reporter protein is provided. | 09-18-2014 |
20140287477 | Variant Maltohexaose-Forming Alpha-Amylase Variants - Disclosed are compositions and methods relating to variant maltohexaose-forming alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing. | 09-25-2014 |
20140295522 | STORAGE-STABLE LIQUID DETERGENT OR CLEANING AGENT CONTAINING PROTEASE AND AMYLASE - The intention is to improve storage stability in a liquid washing or cleaning agent which contains a protease and amylase. This is achieved by using a protease which comprises an amino acid sequence which, over the entire length thereof, is at least 70% identical to the amino acid sequence stated in SEQ ID no. 1 and, in the numbering according to SEQ ID no. 1, has the amino acid substitution R99E or R99D in combination with at least two further amino acid substitutions which are selected from the group consisting of S3T, V4I and V199I. | 10-02-2014 |
20140302583 | GENETICALLY INDUCIBLE HYDROGEN PEROXIDE RESISTANCE AND PRODUCTION - DNA constructs as well as methods for the production of unicellular organisms capable of producing hydrogen peroxide resistance proteins are disclosed. DNA constructs as well as methods for integration of the DNA constructs into the genomes of unicellular organisms for the expression of hydrogen peroxide production proteins are also disclosed. In addition, DNA constructs as well as methods for integration of the DNA constructs into the genomes of unicellular organisms for the expression of hydrogen peroxide resistance and hydrogen peroxide production proteins are disclosed. | 10-09-2014 |
20140308731 | Novel Cytochrome P450 Enzymes From Sorghum Bicolor - Two novel cytochrome P450 genes are isolated from sorghum, each gene encoding a protein having pentadecatrienyl resorcinol hydroxylase activity. Expression vectors containing these sequences are made and used to elevate levels of pentadecatrienyl resorcinol hydroxylase in transgenic cells and organisms. | 10-16-2014 |
20140308732 | KETOREDUCTASE-MEDIATED STEREOSELECTIVE ROUTE TO ALPHA CHLOROALCOHOLS - The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of α-chloroalcohols from α-chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides | 10-16-2014 |
20140322792 | CYTOCHROME P450 OXYGENASES - Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3. | 10-30-2014 |
20140363874 | METHOD FOR PRODUCING LACCASE BY MEANS OF LIQUID FERMENTATION OF LENTINUS EDODES - A method for producing laccase by liquid fermentation of | 12-11-2014 |
20140377839 | MONOATOMIC POINT MUTANT ARTIFICIAL OXIDOREDUCTASES - This invention in biotechnology/bioinformatics claims artificial mutant polypeptides of an oxidoreductase wherein the wild-type (unmutated) oxidoreductase is human catalase (hcat), a superoxide dismutase isoenzyme of superoxide dismutase 2 (hsod2) or superoxide dismutase 3 (hsod3), or a human glutathione peroxidase isoenzyme of human glutathione peroxidase 1 isoform 1 (hgpx1-1), human glutathione peroxidase 1 isoform 2 (hgpx1-2), human glutathione peroxidase 2 (hgpx2), or human glutathione peroxidase 3 (hgpx3). Disclosed in the specification are the methods by which the monoatomic point mutant libraries have been constructed, and the claimed oxidoreductase polypeptides' encoding nucleic acids and recombinant cells thereof. The monoatomic point mutant method generates artificial polypeptides with altered function whilst limiting primary structural obfuscation. The claimed products have multiple potential industrial applications including as novel therapeutics and industrial catalysts. | 12-25-2014 |
20150072397 | GENE ENCODING CELLULASE - Polynucleotide sequences are provided encoding a thermostable cellulase and directing its increased expression are provided, and the use of the thermostable cellulase in hydraulic fracturing methods and the treatment of flowback fluids. | 03-12-2015 |
20150087047 | PROCESS FOR PRODUCTION OF RECOMBINANT PROTEINS AS A SOLUBLE FORM - A target protein is prepared as soluble protein using a recombinant protein expression system. An expression vector is used that includes (1) an expression-inducible promoter sequence; (2) a first coding sequence including a polynucleotide coding for a polypeptide that is represented by the formula (Z) | 03-26-2015 |
20150344852 | DIAPHORASE - [Object] To provide: a novel diaphorase; a method for producing the diaphorase; and use of the diaphorase. | 12-03-2015 |
20150376579 | Methods Of Stapling And Unstapling Peptides And Proteins - The present disclosure pertains to the field peptide stapling and/or macrocyclization, where a structural motif is used to improve the properties of amino acid sequences (e.g. protease resistance, cellular penetration, biological activity). Also within the scope of the disclosure are methods for unstapling the S,S-tetrazine-containing amino acid sequence. The disclosure is also directed to methods for the reductive removal of thiocyanates from an amino acid sequence with cysteine to recycle back to the native amino acid sequence. | 12-31-2015 |
20160010065 | NOVEL P450-BM3 VARIANTS WITH IMPROVED ACTIVITY | 01-14-2016 |
20160024482 | ALKANE OXIDATION BY MODIFIED HYDROXYLASES - This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells. | 01-28-2016 |
20160053236 | COMPOSITION AND METHODS FOR THE PRODUCTION OF L-HOMOALANINE - Healthcare costs are a significant worldwide, with many patients being denied medications because of their high prices. One approach to addressing this problem involves the biosynthesis of chiral drug intermediates, an environmentally friendly solution that can be used to generate pharmaceuticals at much lower costs than conventional techniques. In this context, embodiments of the invention comprise methods and materials designed to allow microorganisms to biosynthesize the nonnatural amino acid L-homoalanine. As is known in the art, L-homoalanine is a chiral precursor of a variety of pharmaceutically valuable compounds including the anticonvulsant medications levetiracetam (sold under the trade name Keppra®) and brivaracetam, as well as ethambutol, a bacteriostatic antimycobacterial drug used to treat tuberculosis. Consequently, embodiments of the invention can be used in low cost, environmentally friendly processes to generate these and other valuable compounds. | 02-25-2016 |
20160068847 | POLYNUCLEOTIDES HAVING LEADER SEQUENCE FUNCTION - The present invention relates to isolated polynucleotides having leader sequence function. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods using the polynucleotides for production of polypeptides. | 03-10-2016 |
20160132633 | METHODS AND SYSTEMS FOR PREDICTING MISFOLDED PROTEIN EPITOPES - A method and system to identify an epitope unique to a misfolded form of a protein is provided. Sets of one or more amino acid residues are selected from a model representing the structure of the protein; the free energy of unfolding of each set is determined; and the epitope is identified from the sets having a total probability of unfolding above a minimum probability or a free energy of unfolding below a minimum energy. In other aspects, the invention provides for the use of epitopes identified by the epitope prediction methods, and related antibodies, to diagnose and treat disease and to screen samples for the presence of such epitopes. | 05-12-2016 |
20160145585 | PROTEIN HAVING NADH AND/OR NADPH OXIDASE ACTIVITY - Water-forming NADH oxidase derived from | 05-26-2016 |
20160200760 | MICROPARTICLES FOR CELL DISRUPTION AND/OR BIOMOLECULE RECOVERY | 07-14-2016 |
20160251634 | MUTATED GENES FOR THE CATALYTIC PROTEIN OF OPLOPHORUS LUCIFERASE AND USE THEREOF | 09-01-2016 |