| Entries |
| Document | Title | Date |
|---|
| 20080227169 | SURFACE-BOUND FLUORINATED ESTERS FOR AMINE CAPTURE - A method for immobilizing an amino-containing material to a substrate is described. The method involves providing a tethering compound with two reactive groups: a substrate reactive group and a fluoroalkoxycarbonyl group. The method further involves preparing a substrate-attached tethering group by reacting the substrate reactive group of the tethering compound with a complementary functional group on the surface of a substrate. The substrate-attached tethering group has a fluoroalkoxycarbonyl group that can be reacted with an amino-containing material to form an immobilization group that connects the amino-containing material to the substrate. | 09-18-2008 |
| 20080241903 | In vivo site-sepecific incorporation of N-acetyl-galactosamine amino acids in eubacteria - Methods and compositions for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid having a N-acetylgalactosamine moiety into a protein; optionally, the N-acetylgalactosamine-containing unnatural amino acid can be further modified with additional sugars. | 10-02-2008 |
| 20080248543 | Method For Removing Enzyme and Method of Base Exchange or Hydrolysis of Phospholipid Using the Same - A method of removing an enzyme from a liquid enzyme reaction mixture used in a hydrolysis reaction or a base exchange reaction of a phospholipid is provided. The method includes the step of treating the liquid enzyme reaction mixture with a solvent mixture of water and an organic solvent, wherein the solvent mixture includes an inorganic metal salt, to remove the enzyme. Enzymes included in the reaction product can be easily removed without a treatment such as heating, and thus it becomes possible to easily produce various phospholipids that have a reduced risk of inducing an allergy, that retain a high quality and that have excellent storage stability. | 10-09-2008 |
| 20080248544 | Methods And Compositions For Grafting Functional Loops Into A Protein - The present invention provides targeted enzymes that bind to targets better than the corresponding pre-targeted enzymes bind the target under like conditions, methods of making targeted enzymes, methods of using targeted enzymes to treat diseases, and pharmaceutical compositions comprising targeted enzymes. | 10-09-2008 |
| 20080248545 | Methods for Generating Novel Stabilized Proteins - The disclosure provides methods for identifying and producing stabilized chimeric proteins. | 10-09-2008 |
| 20080274530 | PEPTIDE-FORMING ENZYME GENE - DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus | 11-06-2008 |
| 20080305535 | In vitro amplification of nucleic acid molecules via circular replicons - Methods and compositions suitable for accomplishing the in vitro amplification of nucleic acid molecules via enzymatic means are provided. The preferred means employ circular rather than linear replicons. Means for producing such circular replicons from linear reactants are also provided. | 12-11-2008 |
| 20090011487 | Production of UGPPase - What are disclosed are a purified novel enzyme protein, UGPPase, which naturally occurs in animal cells, its production by means of recombinant technology, an antibody to the enzyme protein, a method of carrying out ELISA for measurement of the amount of UGPPase in an analyte and a method for determination of UDPG in a sample. The one-way enzyme protein catalyses hydrolysis of UDP-glucose, the precursor molecule of glycogen, into glucose-1-phosphate (G1P) and uridine 5′-monophosphate (UMP). | 01-08-2009 |
| 20090011488 | METHODS FOR STORING COMPOSITIONS USEFUL FOR SYNTHESIZING NUCLEIC ACID MOLECULES - In one aspect, the present invention provides methods for storing a composition useful for synthesizing nucleic acid molecules. The methods of this aspect of the invention include the steps of: (a) freezing multiple aliquots of a liquid composition comprising from 1000 units/mL to 5000 units/mL of a reverse transcriptase, or from 10,000 units/mL to 50,000 units/mL of an RNA polymerase, wherein the multiple aliquots of the liquid composition are disposed within multiple receptacles defined by a container body; and (b) a step selected from the group consisting of (1) storing the frozen aliquots at a temperature below −15° C., and (2) drying the frozen aliquots to produce dried aliquots of the composition, wherein each dried aliquot of the composition comprises an amount of water that is less than 0.1% by weight of the dried aliquot, and storing the dried aliquots at a temperature below −15° C. | 01-08-2009 |
| 20090017520 | Process and Materials for Production of Glucosamine - The present invention relates to a method and materials for producing glucosamine by fermentation of a genetically modified microorganism. Included in the present invention are genetically modified microorganisms useful in the present method for producing glucosamine, as well as recombinant nucleic acid molecules and the proteins produces by such recombinant nucleic acid molecules. | 01-15-2009 |
| 20090029436 | SUBSTANTIALLY PURE REVERSE TRANSCRIPTASES AND METHODS OF PRODUCTION THEREOF - The present invention provides substantially pure reverse transcriptases, which are preferably substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in synthesizing, amplifying or sequencing nucleic acid molecules, including through the use of the polymerase chain reaction, particularly RT-PCR. | 01-29-2009 |
| 20090068717 | In vivo incorporation of unnatural amino acids - The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids. | 03-12-2009 |
| 20090075355 | HUMAN SERUM FOR CELL CULTURE - It is intended to provide a serum which contains a large amount of growth factors capable of efficiently promoting the growth of stem cells. A human serum for cell culture which shows a residual ratio of platelets remaining within 20 minutes after blood collection in relation to the whole amount of the platelets is 0% to 20%, and a release ratio of cell growth factors is 20% to 100%. | 03-19-2009 |
| 20090081754 | GENE OF ENZYME HAVING ACTIVITY TO GENERATE LACHRYMATORY FACTOR - It is an object of the present invention to provide isozymes of the lachrymatory factor producing enzyme, the amino acid sequences of these isozymes and a gene that codes for these amino acid sequences, and the present invention relates to three types of isozymes of the lachrymatory factor producing enzyme that contributes to the production of the lachrymatory factor that is present in onions and the like, amino acid sequences indicated by SEQ ID Nos. 1 to 3 which constitute the proteins or polypeptides of these isozymes, DNA indicated by SEQ ID Nos. 4 and 5 which contains base sequences that code for the abovementioned proteins or polypeptides, a method of producing the abovementioned isozymes, a recombinant vector which contains the abovementioned DNA, a transformant formed by transforming a host cell with the abovementioned recombinant vector, a method of producing proteins or polypeptides that have lachrymatory factor producing enzyme activity by culturing the abovementioned host cell, and anti-sense RNA which has a base sequence that is complementary to that of the mRNA corresponding to the abovementioned DNA. | 03-26-2009 |
| 20090081755 | FRAGRANT CONSUMER PRODUCTS COMPRISING OXIDIZING AGENTS - Fragrant consumer products comprising oxidizing agents are described, which may be, for example, detergents or cleaners or, for example, also cosmetics. These consumer products comprise certain minimum amounts of fragrances of certain classes of substances. They are characterized by very good (storage) stability, both with regard to the fragrance of the product, and also with regard to the potency of the oxidizing agent. | 03-26-2009 |
| 20090098629 | NOVEL GENE EXPRESSED IN PROSTATE CANCER - A novel testis-specific gene expressed in human prostate cancer, designated 22P4F11, is described. Analysis of 22P4F11 mRNA expression in normal prostate, prostate tumor xenografts, and a variety of normal tissues indicates that the expression of this gene is testis specific in normal tissues. The 22P4F11 gene is also expressed in human prostate tumors, in some cases at high levels. A full length cDNA encoding 22P4F11 is provided. The 22P4F11 transcript and/or protein may represent a useful diagnostic marker and/or therapeutic target for prostate cancer. | 04-16-2009 |
| 20090111157 | Protein and peptide fragments from mouse telomerase reverse transcriptase - This invention provides for murine telomerase reverse transcriptase (mTERT) enzyme proteins and nucleic acids, including methods for isolating and expressing these nucleic acids and proteins, which have application to the control of cell proliferation and aging, including the control of age-related diseases, such as cancer. | 04-30-2009 |
| 20090137019 | Thermostable luciferases and methods of production - Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits. | 05-28-2009 |
| 20090137020 | METHOD OF REVERSE TRANSCRIPTION - The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. | 05-28-2009 |
| 20090142820 | Directed evolution methods for improving polypeptide folding, solubility and stability - The invention provides directed evolution methods for improving the folding, solubility and stability (including thermostability) characteristics of polypeptides. In one aspect, the invention provides a method for generating folding and stability-enhanced variants of proteins, including but not limited to fluorescent proteins, chromophoric proteins and enzymes. In another aspect, the invention provides methods for generating thermostable variants of a target protein or polypeptide via an internal destabilization baiting strategy. Internally destabilization a protein of interest is achieved by inserting a heterologous, folding-destabilizing sequence (folding interference domain) within DNA encoding the protein of interest, evolving the protein sequences adjacent to the heterologous insertion to overcome the destabilization (using any number of mutagenesis methods), thereby creating a library of variants. The variants in the library are expressed, and those with enhanced folding characteristics selected. | 06-04-2009 |
| 20090148921 | Compositions and methods for enhancing apoptosis - The present invention is directed to compositions of matter useful for the enhancement of apoptosis in mammals and to methods of using those compositions of matter for the same. | 06-11-2009 |
| 20090148922 | Pancreatic Cancer Genes - The present invention provides the art with the DNA coding sequences of polynucleotides that are up-or-down-regulated in cancer and dysplasia. These polynucleotides and encoded proteins or polypeptides can be used in the diagnosis or identification of cancer and dysplasia. Inhibitors of the up-regulated polynucleotides and proteins can decrease the abnormality of cancer and dysplasia. Enhancing the expression of down-regulated polynucleotides or introducing down-regulated proteins to cells can decrease the growth and/or abnormal characteristics of cancer and dysplasia. | 06-11-2009 |
| 20090155879 | RECONSTITUTION OF 5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE ACTIVITY BY FRAGMENT COMPLEMENTATION - The present invention relates to protein fragments of 5-enolpyruvylshikimate-3-phosphate synthase, which are selected from the protein fragment pairs of EPSPS, two such protein fragments can make up full length EPSPS and reconstitute EPSPS activities by complementation without help of any joint structure. The present invention also relates to nucleic acid molecules encoding the protein fragments, expression vectors and cells comprising such nucleic acid molecules. The present invention also relates to methods for reconstituting EPSPS activities by using the fragments or the nucleic acid molecules or the expression vectors of the present invention, as well as methods for dividing the protein fragments of the present invention. | 06-18-2009 |
| 20090197315 | FULLERENE-BASED AMINO ACIDS - The present invention is directed to a series of new compounds, combining the unique properties of fullerenes and bio-active amino acid residues, and to methods for making such compounds. The present invention is directed toward fullerene-based amino acids, and to amino acid residues, peptide chains, proteins, and polypeptides made from such fullerene-based amino acids. The present invention is further directed to amino acid residues, peptide chains, proteins, and polypeptides comprising such fullerene-based amino acids and into which such fullerene-based amino acids have been incorporated. Exemplary compounds have been prepared, and these compounds have been characterized and confirmed with infrared (IR) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry (MS), etc. These new compounds, which are additions to the existing amino acid residue family, may potentially possess useful pharmaceutical application and may provide a new platform for further exploration in cancer therapy, and peptide and protein engineering. | 08-06-2009 |
| 20090239283 | PYROPHOSPHOROLYSIS ACTIVATED POLYMERIZATION (PAP) - A novel method of pyrophosphorolysis activated polymerization (PAP) has been developed. In PAP, pyrophosphorolysis and polymerization by DNA polymerase are coupled serially for each amplification by using an activatable oligonucleotide P* that has a non-extendible 3′-deoxynucleotide at its 3′ terminus. PAP can be applied for exponential amplification or for linear amplification. PAP can be applied to amplification of a rare allele in admixture with one or more wild-type alleles by using an activatable oligonucleotide P* that is an exact match at its 3′ end for the rare allele but has a mismatch at or near its 3′ terminus for the wild-type allele. PAP is inhibited by a mismatch in the 3′ specific sequence as far as 16 nucleotides away from the 3′ terminus. PAP can greatly increase the specificity of detection of an extremely rare mutant allele in the presence of the wild-type allele. Specificity results from both pyrophosphorolysis and polymerization since significant nonspecific amplification requires the combination of mismatch pyrophosphorolysis and misincorporation by the DNA polymerase, an extremely rare event. Using genetically engineered DNA polymerases greatly improves the efficiency of PAP. | 09-24-2009 |
| 20090246851 | METHOD FOR PRODUCTION OF ENZYME - The present invention provides a process for producing an enzyme, which includes recovering an enzyme by microfiltering an enzyme-containing solution, having a cell density of 1% (v/v) or less and an enzyme concentration of 1% (w/v) or more in terms of the amount of proteins, with a cationic surfactant added in an amount of 0.01 to 1% (w/v) to the enzyme-containing solution. | 10-01-2009 |
| 20090246852 | THERAPEUTIC USE OF ANTI-CS1 ANTIBODIES - The present invention is directed to antagonists of CS1 that bind to and neutralize at least one biological activity of CS1. The invention also includes a pharmaceutical composition comprising such antibodies or antigen-binding fragments thereof. The present invention also provides for a method of preventing or treating disease states, including autoimmune disorders and cancer, in a subject in need thereof, comprising administering into said subject an effective amount of such antagonists. | 10-01-2009 |
| 20090263879 | Immunoassay for specific determination of S-adenosylmethionine and analogs thereof in biological samples - This invention pertains to a method for detecting a compound in the presence of other compounds that are substantially similar in structure and metabolically related to the analyte. The invention is particularly suited for the detection of S-adenosylmethionine in the presence of S-adenosylhomocysteine, other nucleosides and derivatives in a biological sample. The methods of this invention involve an antibody produced specifically against S-adenosylmethionine; particularly, analogs modified strategically at the sulfonium position. An assay protocol comprises chemically modified analyte analog linked to an enzymatic reporter and the aforementioned antibody was used to demonstrate the assay specificity and sensitivity. Additional assay method with immobilized immunogen, the specific antibody, and an enzyme labeled secondary antibody was also described for illustration. The invention also features hapten design and novel compounds used as haptens to prepare immunogen and for the specific antibody production. | 10-22-2009 |
| 20090317888 | THERMUS EGERTSSONII DNA POLYMERASES - The present invention relates to a thermophilic polymerase, wherein the DNA polymerase has an in-vitro primer extension rate that is >35 bases/second and faster relative to the primer extension rate of a DNA polymerase comprising amino acid sequences SEQ ID NO: 2 or 4, when measured under identical conditions in a DNA replication assay using primed single strand M13mp18 DNA and an incubation temperature of 60° C. The invention also relates to a vector comprising the polymerase, a host cell comprising the vector. The invention relates to a nucleic acid replication kit comprising the polymerase according to the invention. | 12-24-2009 |
| 20100015683 | THERMOSTABLE REVERSE TRANSCRIPTASES AND USES THEREOF - The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions. | 01-21-2010 |
| 20100062509 | HIGHLY POROUS MAGNETIC TEMPORARY FIXED BED - The present invention relates to a fixed bed, for example for the isolation and/or purification of components originating from a biological system, which fixed bed comprises magnetic beads and a magnetizable fabric arranged at least in part in the fixed bed, a fluidized bed-fixed bed, which comprises the fixed bed of the invention after application of an alternating magnetic field, and also to a process for the isolation and/or purification of components originating from a biological system. | 03-11-2010 |
| 20100062510 | Protein exhibiting activity of pyrethrin biosynthetic enzyme, gene encoding the protein, and vector bearing the gene - The present invention relates to an enzyme determining amino acid sequences of an enzyme involved in pyrethrin biosynthesis and a base sequence of the gene thereof; constructing vectors bearing the gene and transformants; and extractable from plant bodies producing pyrethrin by applying such creative techniques to plant bodies with faster growth aiming to provide a method to efficiently produce pyrethrin; and the enzyme is a gene encoding a protein of the following (i) or (ii):
| 03-11-2010 |
| 20100068785 | SPINOSYN-PRODUCING POLYKETIDE SYNTHASES - The invention provides, biologically active spinosyns, hybrid spinosyn polyketide synthases capable of functioning in | 03-18-2010 |
| 20100086985 | NUCLEIC ACID AND CORRESPONDING PROTEIN ENTITLED 205P1B5 USEFUL IN TREATMENT AND DETECTION OF CANCER - A novel gene (designated 205P1B5) and its encoded protein are described. While 205P1B5 exhibits tissue specific expression in normal adult tissue, it is aberrantly expressed in prostate cancer. Consequently, 205P1B5 provides a diagnostic, prognostic, prophylactic and/or therapeutic target for cancer. The 205P1B5 gene or fragment thereof, or its encoded protein or a fragment thereof, can be used to elicit an immune response. | 04-08-2010 |
| 20100093054 | NOVEL METHODS FOR RECOVERY OF LEAF PROTEINS - A novel method for processing soluble plant leaf proteins is described. While leaf proteins are considered potentially the most abundant source of protein in nature, the lack of efficient processing techniques for leaf proteins has limited their commercial use. The method described in this patent provides a means of extracting and purifying leaf proteins from plants which is suitable for leaf protein production on an industrial scale. | 04-15-2010 |
| 20100093055 | REMEDY FOR DIABETES - A method of screening a compound having a hypoglycemic effect (hereinafter referred to as “hypoglycemic compound”), a remedy for diabetes which contains a compound having a novel function mechanism, etc. More specifically speaking, a method of screening a hypoglycemic compound capable of binding to the β subunit of a trimeric GTP-binding protein, a remedy for diabetes comprising a hypoglycemic compound, which is characterized by being capable of binding to the β subunit of a trimeric GTP-binding protein, as the active ingredient, etc. | 04-15-2010 |
| 20100129889 | AFFINITY SEPARATION METHODS AND SYSTEMS - An affinity matrix comprising a base matrix containing biotin; and a fusion protein attached to the base matrix, wherein the fusion protein contains a matrix binding element capable of binding to the base matrix via biotin and a target binding element capable of binding, or being bound by, at least one target component. | 05-27-2010 |
| 20100144008 | TREATMENT OF FABRY DISEASE - A pathogenic factor in plasma as an improved therapy for Fabry disease. | 06-10-2010 |
| 20100159558 | REDUCING BYPRODUCTION OF MALONATES IN A FERMENTATION PROCESS - Described are methods of reducing the amount of byproduct organic acids during fermentation of an organism, based on expression of a heterologous malonyl-CoA synthetase. A polyunsaturated fatty acid [“PUFA”]-producing strain of the oleaginous yeast | 06-24-2010 |
| 20100167374 | ULTRA-FAST CHROMATOGRAPHY - The present invention relates to a chromatographic method of separating biological material comprising, providing chromatographic media comprising inorganic oxide particles having an average diameter of about 2 microns or less and an average pore diameter of 300 Å or more; applying a solvent comprising said biological material to said media, wherein said biological material is reversibly bonded to said media; and eluting said biological material from said media with a solvent in less than about 2 minutes for biological material having a molecular weight of less than about 100,000 Daltons. | 07-01-2010 |
| 20100167375 | Novel Lipolytic Enzyme LIP2 - The present invention provides a novel nucleic acid sequence, designated LIP2, encoding a lipolytic enzyme and the corresponding encoded amino acid sequences. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding at least one novel lipolytic enzyme, recombinant lipolytic enzyme proteins and methods for producing the same. | 07-01-2010 |
| 20100173378 | AVIAN TELOMERASE REVERSE TRANSCIPTASE - The present invention notably relates to novel recombinant telomerase reverse transcriptases, nucleic acid molecules coding them, cells comprising said nucleic acid molecule and use of these cells for the production of substance of interest. | 07-08-2010 |
| 20100173379 | Compositions of Aminoacyl-tRNA Synthetase and Uses Thereof - Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNA's/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs. | 07-08-2010 |
| 20100173380 | Compositions of Aminoacyl-tRNA Synthetase and Uses Thereof - Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNA's, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNA's/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs. | 07-08-2010 |
| 20100203612 | SPECIFIC PEPTIDE BINDING TO GLYPICAN-3 - A specific targeting peptide binding to glypican-3 can specifically binds to glypican-3 overexpressed in carcinoma cells and includes an amino acid sequence represented by SEQ ID No. 1 or some thereof. Glypican-3 is overexpressed in malignant tumors including hepatocellular carcinoma, melanoma, germ cell tumor, etc., and may be targeted in diagnosis and treatment of tumors by marking the targeting peptide. A diagnosis using the targeting peptide may detect even small tumors more accurately than conventional methods. A treatment method using the targeting peptide may remove only carcinoma cells without harming other normal tissues. | 08-12-2010 |
| 20100209990 | 11 BETA HYDROXYSTEROID DEHYDROGENASE TYPE 1 - The invention provides gene-targeted non-human animals comprising a genetically modified 11βHSD1 gene encodes a mutant 11βHSD1 polypeptide which is modulated by human 11βHSD1 modulating compounds. The invention further provides cells expressing mutant 11βHSD1 and cells isolated from gene-targeted animals, which cells produce a mutant 11βHSD1. The invention further provides methods of identifying agents that modulate 11βHSD1 activity, and are useful to treat 11βHSD1-related metabolic disorders. | 08-19-2010 |
| 20100209991 | IONIC LIQUIDS DERIVED FROM FUNCTIONALIZED ANIONIC SURFACTANTS - A novel class of ionic liquids and methods for their preparation are disclosed. Specifically, these novel ionic liquids can be derived from anionic surfactants, such as alkyl aryl sulfonates, and mid-chain branched derivatives of alkyl sulfates, alkyl alkoxy sulfates, and alkyl aryl sulfonates. In addition, novel ionic liquids can be derived from other anionic surfactants, such as methyl ester sulfonates (MES), alkyl glycerol ether sulfonates, and alpha olefin sulfonates. Anions may be paired with a variety of cations to achieve various advantageous properties. The present invention also relates to compositions containing these novel ionic liquids and method of using the same. | 08-19-2010 |
| 20100209992 | Process for the production of a catalyst preparation and use of the catalyst preparation - A process is described for the production of a catalyst preparation, in which the catalyst containing at least one inorganic compound which is solid under standard conditions is comminuted by means of a dispersion unit into particles having a maximum average particle size d | 08-19-2010 |
| 20100216211 | FIBROUS MATS CONTAINING CHITOSAN NANOFIBERS - The invention relate to fibrous mats comprising chitosan nanofibers and, optionally, at least one filler material, at least one additive, or both. The invention also relates to methods of making same, and devices that include a fibrous mat comprising chitosan nanofibers. | 08-26-2010 |
| 20100227373 | Recombinant Carrier Molecule for Expression, Delivery and Purification of Target Polypeptides - Recombinant carrier molecules having amino acid sequences from thermostable enzymes and methods of use for expression, recovery and delivery of foreign sequences (peptides and polypeptides) produced in different systems (bacteria, yeast, DNA, cell cultures such as mammalian, plant, insect cell cultures, protoplast and whole plants in vitro or in vivo are provided. The recombinant carrier molecule using sequences from lichenase B(Lic B) were also made and used as part of carrier protein to express, recover and deliver a variety of target polypeptides of interest. | 09-09-2010 |
| 20100261245 | LONG ACTING FORMULATION OF BIOPHARMACEUTICAL - The invention relates to a long-acting formulation of biopharmaceutical, more specifically an aptamer therapeutics. A branched PEGylated aptamer or a hyaluronic acid (HA) derivative of which degradation in vivo is regulated is linked by the bioconjugation with biopharmaceutical to produce the long-action formulation. | 10-14-2010 |
| 20100267109 | Spatially Inhomogenously Functionalized Porous Media and Method for Use in Selective Removal of Contaminants - Compositions and methods for separating double-stranded nucleic acids out of a mixture comprising single-stranded nucleic acids and/or dNTPs and/or enzymes. The method uses spatially inhomogenously functionalized nanoporous materials. For example, the compositions and methods of the present invention can be used to purify DNA amplification reaction products. | 10-21-2010 |
| 20100273232 | METHODS FOR CLONING SMALL RNA SPECIES - This invention pertains to methods for cloning microRNA (miRNA) and other small ribonucleic acid (RNA) species from relevant cell sources. | 10-28-2010 |
| 20100285562 | ISOCYCLOMALTOOLIGOSACCHARIDE(S), ISOCYCLOMALTOOLIGOSACCHARIDE-FORMING ENZYME, THEIR PREPARATION AND USES - A non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents, a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof are provided by use of an isocyclomaltooligosaccharide(s) having a structure represented by Formula 1, | 11-11-2010 |
| 20100285563 | Non-Ribosomal Peptide Synthetases - Novel tailor-made artificial non-ribosomal peptide synthetases (NRPSs) for non-ribosomal synthesis and/or modification of peptides of a predetermined length and composition and/or for modification of individual amino acids are described. The fusion of building units of said peptide synthetases in particular linker regions makes it possible to specifically prepare by means of “modular molecule construction kits” NRPSs which are capable of synthesizing peptides of a desired structure. | 11-11-2010 |
| 20110008866 | Tangential Flow Filtration Apparatuses, Systems, and Processes for the Separation of Compounds - The invention relates to apparatuses, machines, systems and methods for the recovery and purification of proteins, peptides, nucleic acids, biologically produced polymers and other compounds from aqueous fluids. The aqueous fluids can comprise enzyme concentrates and or a fermentation broth with or without cells or other starting material. The fermentation broth can be produced by fermentations of fungal, yeast, bacterial, mammalian, insect or plant cells. | 01-13-2011 |
| 20110027855 | Maize Cellulose Synthases and Uses Thereof - The invention provides isolated cellulose synthase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering cellulose synthase levels in plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants comprising said nucleic acids. | 02-03-2011 |
| 20110059501 | Protein Glycosylation - The present invention relates to methods for glycosylating a protein in which the protein is modified to include an alkyne and/or an azide group. The invention further relates to a protein glycosylated by these methods. | 03-10-2011 |
| 20110070621 | Multivalent Clostridial Toxins - The present invention is directed to multivalent Clostridial toxin comprising more than one binding domain directed to a cell surface molecule of a target cell. Such modified toxins are useful as therapeutic compositions to prevent exocytosis and secretion by the target cell. Conditions in which such compositions may be useful include, without limitation, disorders of the sensory or motor nervous system, acute or chronic pain, cancer, pancreatitis, hyperhydrosis, glandular disorders, viral infections, cystic fibrosis and the like. The invention is also directed to methods of using and administering such a composition, and methods of treating a given condition using such a composition. | 03-24-2011 |
| 20110104781 | ISOLATED PHOSPHOLIPID-PROTEIN PARTICLES - Systems and methods are provided for producing a protein of interest that is typically not amenable to expression in soluble form in in vitro expression systems. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include a scaffold protein such as apolipoprotein or an amphipathic alpha helix containing (“AAHC”) protein, in which higher yields of soluble protein are produced than in the absence of the scaffold protein. The scaffold proteins may be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The scaffold protein may be provided as a protein per se or may be encoded by a nucleic acid template and co-expressed with the protein of interest. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein expression and isolation. | 05-05-2011 |
| 20110111477 | LONG ACTING FORMULATION OF BIOPHARMACEUTICAL - The invention relates to a long-acting formulation of biopharmaceutical, more specifically an aptamer therapeutics. A branched PEGylated aptamer or a hyaluronic acid (HA) derivative of which degradation in vivo is regulated is linked by the bioconjugation with biopharmaceutical to produce the long-action formulation. | 05-12-2011 |
| 20110124080 | Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells - This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation. | 05-26-2011 |
| 20110136200 | Bacterial Glutamine Synthetases and Methods of Use - Compositions and methods for conferring herbicide resistance to and improving nitrogen utilization of bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to herbicidal glutamine synthetase inhibitors are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides corresponding to herbicidal glutamine synthetase inhibitor-resistant polynucleotides are provided. Additionally, polypeptides corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising a variant of SEQ ID NO:1, wherein the variant polynucleotide encodes a polypeptide that is resistant to inhibition by herbicidal glutamine synthetase inhibitor. | 06-09-2011 |
| 20110183399 | Maize Cellulose Synthases and Uses Thereof - The invention provides isolated cellulose synthase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering cellulose synthase levels in plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants comprising said nucleic acids. | 07-28-2011 |
| 20110195475 | ARTIFICIAL ENZYMES - The present invention is directed to the use of artificial polymers in the mimetization of enzymatic active sites and the carrying-out of catalysis using these artificial enzymes. Further, as used herein, an artificial enzyme refers more generally to a polymer-based scaffold for presenting specific chemically active atoms optimally for reactions, not just those that mimic natural enzymes. Various polymers can be used for this mimetization, including polyimides, polyurea, polyurethane, polyacrylic acid, and polylactic acid, as well as other polymers having properties and functionality that enable integration with natural and artificial amino acids, other molecules having nucleophilic and electrophilic groups (akin to the amine and carboxyl functionalities, respectively, of amino acids), as well as other molecules contributing unique chemical abilities not usually associated with the orthogonal functions inherent in most amino acids, i.e., amines, carboxyls, formamides, hydroxyls, mercaptyls and saturated hydrocarbons. | 08-11-2011 |
| 20110195476 | MODIFIED CHONDROITIN SYNTHASE POLYPEPTIDE AND CRYSTAL THEREOF - Disclosed are: (A) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2, or (B) a polypeptide comprising an amino acid sequence of SEQ ID NO:2 including deletion, substitution or addition of one or several amino acid residues and having chondroitin synthase activity; a nucleic acid encoding the polypeptide; a method for producing the polypeptide, comprising at least the steps of: (1) expressing the nucleic acid to produce the polypeptide; and (2) collecting the polypeptide produced in the step (1); and a crystal of the polypeptide. The crystal may be a monoclinic or tetragonal crystal. | 08-11-2011 |
| 20110201078 | LIGAND FUNCTIONALIZED POLYMERS - Ligand functionalized substrates, methods of making ligand functionalized substrates, and methods of using functionalized substrates are disclosed. | 08-18-2011 |
| 20110201079 | IDENTIFICATION AND MODIFICATION OF DYNAMICAL REGIONS IN PROTEINS FOR ALTERATION OF ENZYME CATALYTIC EFFECT - A method for analysis, control, and manipulation for improvement of the chemical reaction rate of a protein-mediated reaction is provided. Enzymes, which typically comprise protein molecules, are very efficient catalysts that enhance chemical reaction rates by many orders of magnitude. Enzymes are widely used for a number of functions in chemical, biochemical, pharmaceutical, and other purposes. The method identifies key protein vibration modes that control the chemical reaction rate of the protein-mediated reaction, providing identification of the factors that enable the enzymes to achieve the high rate of reaction enhancement. By controlling these factors, the function of enzymes may be modulated, i.e., the activity can either be increased for faster enzyme reaction or it can be decreased when a slower enzyme is desired. This method provides an inexpensive and efficient solution by utilizing computer simulations, in combination with available experimental data, to build suitable models and investigate the enzyme activity. | 08-18-2011 |
| 20110207196 | DEPTH FILTER LAYER WITH INORGANIC LAYER DOUBLE HYDROXIDE - The present invention relates to a depth filter layer with inorganic layer double hydroxide, a method for production thereof, a filtration device containing this, a method for the separation of at least one contaminant from at least one target product in a liquid medium by means of at least one upstream depth filter layer and the use of the depth filter layer for removal of contaminants. The depth filter layer selectively retains contaminants such as nucleic acids, while target proteins of biotechnological processes are transmissible into the filtrate. | 08-25-2011 |
| 20110217752 | LIGAND FUNCTIONALIZED POLYMERS - Ligand functionalized substrates, methods of making ligand functionalized substrates, and methods of using functionalized substrates are disclosed. | 09-08-2011 |
| 20110236950 | GRG23 EPSP SYNTHASES: COMPOSITIONS AND METHODS OF USE - Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34. | 09-29-2011 |
| 20110275133 | Proteinaceous Digestates - A method of aiding the digestion of a proteinaceous material, the method comprising adding to a sample of proteinaceous raw material a composition comprising fruit, fruit waste or an enzyme derived therefrom. A digestate liquor obtained by said method is also described. | 11-10-2011 |
| 20110294188 | Ideotypically Modulated Pharmacoeffectors For Selective Cell Treatment - In a method embodiment, a method includes introducing a plurality of Ideotypically Modulated Pharmacoeffectors (IMP) into a population of cells. Each IMP may include a detection domain and an activation domain. One or more epitopes is bound by the detection domain. The activation domain is activated in response to the binding. Applications may include but are not limited to viral infections, other intracellular infections, cancers, vector-borne diseases, autoimmune diseases, cellular diseases, cellular enhancement, and research. | 12-01-2011 |
| 20110318806 | SITE SPECIFIC INCORPORATION OF KETO AMINO ACIDS INTO PROTEINS - Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate keto amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with keto amino acids using these orthogonal pairs. | 12-29-2011 |
| 20120003712 | METHOD FOR PREPARING A SITE-SPECIFIC PHYSIOLOGICALLY ACTIVE POLYPEPTIDE CONJUGATE - The present invention provides a method for preparing a site-specific physiologically active polypeptide conjugate in a high yield by treating a physiologically active polypeptide with a non-peptidyl polymer in the presence of an alcohol at a specific pH, which can be desirably employed in the development of long acting formulations of various peptide drugs having high in-vivo activity and markedly prolonged in-blood half-life. | 01-05-2012 |
| 20120021484 | Recombinant FcRn and Variants Thereof for Purification of Fc-Containing Fusion Proteins - The invention is directed to methods of purifying Fc-containing molecules using a soluble neonatal Fc receptor (sFcRn). Native FcRn binds Fc-containing proteins at or below about pH 6.5 and releases them at or above about pH 7 and provides a much milder approach for capturing and purifying Fc-containing proteins, in particular, therapeutic Fc-containing proteins. Other embodiments of the invention provide modifications to alter the pH for binding and elution to the sFcRn, to modulate Fc-containing protein binding affinity, to affect sFcRn linkage to a support surface, or to improve the stability of sFcRn to conditions utilized in the methods of the invention. | 01-26-2012 |
| 20120028329 | OPTICALLY TRANSPARENT GLASS AND GLASS-CERAMIC FOAMS, METHOD FOR PRODUCTION THEREOF AND USE THEREOF - The present invention relates to an optically transparent glass foam or glass-ceramic foam as well as to a method for the production of an optically transparent glass foam or glass-ceramic foam. A method is described for the production of optically transparent foams, wherein the following steps are conducted: | 02-02-2012 |
| 20120028330 | POLYPEPTIDE HAVING ACTIVITY OF AMINOACYL-tRNA SYNTHETASE AND USE THEREOF - A polypeptide according to the present invention includes: an altered polypeptide obtained by altering an ArgRS, a CysRS, a MetRS, a GlnRS, a GluRS, a LysRS, a TyrRS, or a TrpRS so that an unnatural amino acid is recognized; and an editing polypeptide derived from a PheRS, a LeuRS, an IleRS, a ValRS, an AlaRS, a ProRS, or a ThrRS, the editing polypeptide having been either inserted between a Rossman-fold N domain and a Rossman-fold C domain that exist in the altered polypeptide, or bound to an N terminal of the altered polypeptide. Thus provided are a new aaRS that exhibits high substrate specificity to an unnatural amino acid and a technique that involves the use of such an aaRS. | 02-02-2012 |
| 20120028331 | ENZYMATIC REACTION REAGENT, ENZYMATIC REACTION REAGENT KIT AND METHOD FOR STORING LIQUID FOR ENZYMATIC REACTION - An enzymatic reaction reagent prepared by freezing a liquid for enzymatic reaction that is divided into a plurality of constituent liquids, wherein at least one of the constituent liquids contains an enzyme, each of the constituent liquids is frozen individually, and all of the constituent liquids are encased in a single container. Also, an enzymatic reaction reagent kit containing the reagent, and a method for storing a liquid for an enzymatic reaction that has been divided into a plurality of constituent liquids, wherein at least one of the constituent liquids contains an enzyme, each of the constituent liquids is frozen individually in succession, all of the constituent liquids are encased in a single container, and the container is stored in a frozen state. The invention can provide an enzymatic reaction reagent that exhibits excellent storage stability of the enzyme, can simplify the operations required during use of the reagent, and can reduce reagent loss and raw material costs, as well as providing an enzymatic reaction reagent kit that contains the reagent, and a method for storing a liquid for an enzymatic reaction. | 02-02-2012 |
| 20120045815 | CRYSTAL STRUCTURE OF GLUTAMINYL CYCLASE - A novel crystal structures of human and murine glutaminyl cyclase (QC, EC 2.3.2.5), methods of preparing the crystals, as well as the use of said crystal structures for identifying inhibitors of human and murine glutaminyl cyclase. | 02-23-2012 |
| 20120058535 | BIOFUEL PRODUCTION IN PROKARYOTES AND EUKARYOTES - Terpene synthases are enzymes that directly convert IPP & DMAPP to terpenes, such as fusicoccadiene. Described herein are methods and compositions for the production of terpenes and terpenoids for use as fuel molecules or other useful components. Genetically engineered enzymes capable of producing terpenes and terpenoids are also described. | 03-08-2012 |
| 20120064598 | Mutation of the parkin gene, compositions, methods and uses - The invention concerns nucleic acids coding for mutated or truncated forms of the human parkin gene, or forms comprising multiplication of exons, and the corresponding proteins and antibodies. The invention also concerns methods and kits for identifying mutations of the parkin gene, and for studying compounds for therapeutic purposes. | 03-15-2012 |
| 20120083024 | Materials and Methods for Synthesis of a Flavor and Aroma Volatile in Plants - The subject invention concerns polynucleotides encoding a plant 2-phenylethanol dehydrogenase enzyme. In one embodiment, the polynucleotide encodes a tomato 2-phenylethanol dehydrogenase. The subject invention also concerns polynucleotides encoding a plant phenylalanine decarboxylase enzyme. In one embodiment, the polynucleotide encodes a tomato phenylalanine decarboxylase. The subject invention also concerns 2-phenylethanol dehydrogenase polypeptides and phenylalanine decarboxylase polypeptides encoded by polynucleotides of the present invention. The subject invention also concerns methods for providing a plant with an increased flavor and aroma volatile. Plants can be transformed with one or more polynucleotide of the present invention. The subject invention also concerns these transformed plant cells, plant tissue, and plants and transgenic progeny thereof. | 04-05-2012 |
| 20120094355 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids. | 04-19-2012 |
| 20120122178 | Human Aminoacyl-tRNA Synthetase Polypeptides Useful for the Regulation of Angiogenesis - The present invention provides an isolated polypeptide comprising residues 94-471 of SEQ ID NO: 10, wherein the isolated polypeptide does not comprise amino acid residues 1-47 of SEQ ID NO: 10. Nucleic acids and expression vectors encoding the peptide also are provided. | 05-17-2012 |
| 20120208257 | ADDITIVE FOR BIOLEACHING THAT IS SUBSTANTIALLY MADE UP OF THE LICANANTASE LIPOPROTEIN, AND BIOLEACHING PROCESS TO WHICH THIS ADDITIVE IS ADDED TO INCREASE THE RECOVERY OF COPPER - The invention discloses an additive for bioleaching that makes it possible to increase the recovery of copper from sulfide ores. In which this additive is substantially made up of the Licanantase lipoprotein and a solution of sulfuric acid with a pH of 0.8 to 3. The Licanantase lipoprotein that has an amino acid sequence with at least 50% homology regarding the sequence defined in SEQ ID No. 1 or is the product of translation of a nucleotide sequence with at least 50% homology regarding the sequence defined in SEQ ID No. 2. It also protects the improved bioleaching process that includes adding the additive during the ore bioleaching process as defined in the present invention; and continuing with the habitual process, obtaining copper recoveries increased 5 to 20%. | 08-16-2012 |
| 20120220008 | NUCLEIC ACIDS AND POLYPEPTIDES INVOLVED IN THE PRODUCTION OF CRYPTOPHYCIN - The present invention provides polypeptides involved in cryptophycin biosynthesis and the nucleic acid molecules that encode such polypeptides. The nucleic acid molecules and polypeptides of the invention or variants thereof can be used in the methods of the invention to produce cryptophycins. | 08-30-2012 |
| 20120220009 | Cross-Flow Membrane Filtration-Based Process For Protein Recovery - The present methods relate to the isolation and concentration of proteins using cross-flow membrane filtration, and to proteins produced by such methods. A feature of the method is that the protein of interest is retained by a cross-flow membrane under certain conditions that promote retention, while under other condition the protein passes through the membrane. | 08-30-2012 |
| 20120237998 | Conjugation Reactions - We describe methods that allow either carbodiimides or other carboxyl-reactive substances to be mixed with solutions of carboxylic acids or phosphates or amines or combinations thereof, so as to form a homogeneous mixture which is then dried, preferably in a freeze drying process. The mixture is then contacted with an entity, which preferably involves the dissolution of the mixture with a buffered solution of the entity, so as to initiate a conjugation reaction between the entity and a component in the mixture. | 09-20-2012 |
| 20120252091 | LIGAND FUNCTIONAL SUBSTRATES - A substrate comprising a crosslinked polymer primer layer, and grafted thereto a ligand-functionalized polymer is provided. The grafted polymer has the requisite affinity for binding neutral or negatively charged biomaterials, such as cells, cell debris, bacteria, spores, viruses, nucleic acids, and proteins, at pH's near or below the pI's of the biomaterials. | 10-04-2012 |
| 20120282668 | Genetically Programmed Expression of Proteins Containing the Unnatural Amino Acid Phenylselenocysteine - The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate the unnatural amino acid phenylselenocysteine into proteins produced in eubacterial host cells such as | 11-08-2012 |
| 20130040358 | VIAL USEABLE IN TISSUE EXTRACTION PROCEDURES - Disclosed is a device useful for preserving tissue samples after extraction from human or animal patients. The device includes a circular blade for taking a tissue sample, the blade being coupled to a sealing cap which mates to one end of a container. A retrieval port is also disclosed for extracting fluids from the container. Methods are also disclosed by which tissue samples may be taken and prepared for storage, processing, culture, or other analysis. | 02-14-2013 |
| 20130078704 | Processing Chemicals - Methods of processing chemicals change their structure, and in particular increase their solubility and/or rate of dissolution, for intermediates and products made from the structurally changed materials. Many of the methods provide materials that can be more readily utilized in reactions or other processes to produce useful intermediates and products, e.g., energy, fuels, foods or materials. Chemicals that are treated using the processes described herein can be used to form highly concentrated solutions. Treatment can change the functionality of the chemical, and thus the polarity of the chemical, which may render the treated chemical soluble in solvents in which the untreated chemical is insoluble or only sparingly or partially soluble. Methods may in some cases increase the solubility of the chemical in water or aqueous media. The chemical may be, for example, a solid, liquid, or gel, or mixtures thereof. | 03-28-2013 |
| 20130171713 | WASTE DISPOSAL APPARATUS, FLUID AND METHOD - A waste disposal apparatus, for disposing of waste materials using aerobic decomposition, includes a decomposition chamber having a waste inlet for receiving waste materials, and a closure member for closing the inlet and sealing the decomposition chamber. The apparatus includes a stirrer for stirring waste materials in the chamber, and a waste outlet for discharging waste materials from the chamber after aerobic decomposition thereof. | 07-04-2013 |
| 20130183735 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids. | 07-18-2013 |
| 20130183736 | Process for Designing, Constructing, and Characterizing Fusion Enzymes for Operation in an Industrial Process - A method and a software tool is achieved that measures the distance between a parameter vector that describes an industrial process and vectors that characterize different enzymatic activities. A distance matrix is built and used to construct a hierarchical binary cluster tree of parameter vectors. A novel scoring system is used to rank the hierarchically grouped enzymes. To select the best enzymes for the chimeric fusion enzyme for a particular industrial process, the novel scoring system takes into account biochemical and biophysical variables by creating a belief system. The scoring is generated by summing the products of a biochemical/biophysical variable and its belief parameters/weights for those enzymes found by the distance matrix to have enzymatic activities closest to the enzymatic activities of the industrial process. These scores are then used to select the best enzymes for the particular industrial process. | 07-18-2013 |
| 20130210112 | BIOREMEDIATION METHOD FOR ACCELERATED BIOLOGICAL DECOMPOSITION OF PETROLEUM HYDROCARBONS IN SEA ICE-COVERED POLAR REGIONS, AND BACTERIA AND ENZYME MIXTURES AS AGENTS FOR CARRYING OUT SAID METHOD - A bacterial mixture usable in an inoculum usable in a bioremediateion method for accelerated biological degradation of petroleum hydrocarbons in a sea ice-covered polar region includes a plurality of isolated cold-adapted autochthonous bacterial strains. Each of the bacterial strains has petroleum hydrocarbons degrading activity at an ambient temperature of −3° C. and each has a different temperature tolerance range, a different salinity tolerance range, a different petroleum hydrocarbons degradation spectrum, and a different capacity to emulsify oil. | 08-15-2013 |
| 20130267005 | MICROFLUIDIC FLUID SEPARATOR AND RELATED METHODS - A microfluidic fluid separator for separating target components of a fluid by filtration is described. Methods for separating target components of a fluid by filtration and methods for processing blood on a large scale with the microfluidic fluid separator are provided. | 10-10-2013 |
| 20130344562 | PROTEIN SYNTHESIS MODULATORS - The invention provides a high resolution three-dimensional structure of cycloheximide, either alone or in association with a large ribosomal subunit. The invention provides methods for designing and/or identifying cycloheximide analogs and derivatives that bind and/or modulate the protein biosynthetic activity of the ribosome. | 12-26-2013 |
| 20140065690 | Avian Transgenesis and Exogenous Proteins - This invention provides vectors and methods for the stable introduction of exogenous nucleic acid sequences into the genome of a bird and for expressing said exogenous sequences to alter the phenotype of the bird or to produce desired proteins. In particular, transgenic chickens are produced which express exogenous sequences in their oviducts. Eggs which contain exogenous proteins are also produced. | 03-06-2014 |
| 20140106431 | HIGH FIDELITY THERMOSTABLE LIGASE AND USES THEREOF - The present invention is directed to a mutant thermostable ligase having substantially higher fidelity than either T4 ligase or | 04-17-2014 |
| 20140147904 | FILTERING APPARATUS AND METHOD FOR MIXING, EXTRACTION AND/OR SEPARATION - The present invention relates to a filtering apparatus and method for mixing a compound of solid and fluid phases, separating the phases and/or extracting fluid from the compound. One embodiment of the invention discloses a filtering apparatus comprising a first filter section accommodating a first group of filter members, and a second filter section accommodating a second group of filter members, a piping system providing pipelined fluid communication between the filter sections and between a filter section and said filter section's corresponding group of filter members, the piping system configured such that the filter members form filtered fluid openings of the filtering apparatus, and circulation means, such as a pump, configured for passing fluid in a forward flow and/or in a reverse flow between the filter sections. In particular the invention may be used for the mashing process in a beer brewing procedure. | 05-29-2014 |
| 20140302581 | METHODS FOR ENHANCED PROTEIN PRODUCTION - The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein (e.g., the growth phase) in a perfusion cell culture to a high cell density (i.e., at least above about 40×10 | 10-09-2014 |
| 20140308729 | Novel Method of Protein Purification - The present disclosure provides methods for releasing intracellular proteins. The method allows isolation of the protein of interest from the cell without the requirement for mechanical disruption of the cells, without the need for isolation of the cells from the culture media, and without the need for removal of the cells from the culture media. | 10-16-2014 |
| 20140335589 | SEPARATION OF A TARGET SUBSTANCE FROM A FLUID OR MIXTURE USING ENCAPSULATED SORBENTS - Method and apparatus for separating a target substance from a fluid or mixture. Capsules having a coating and stripping solvents encapsulated in the capsules are provided. The coating is permeable to the target substance. The capsules having a coating and stripping solvents encapsulated in the capsules are exposed to the fluid or mixture. The target substance migrates through the coating and is taken up by the stripping solvents. The target substance is separated from the fluid or mixture by driving off the target substance from the capsules. | 11-13-2014 |
| 20140377835 | METHODS AND COMPOSITIONS FOR ISOLATING, IDENTIFYING AND CHARACTERIZING MONOCOT PLASTIDIC ACCASE HERBICIDE TOLERANT MUTATIONS USING A MODEL SYSTEM - The present invention relates compositions and methods for identifying, isolating, and characterizing herbicide tolerant mutations in monocot plastidic acetyl-CoA carboxylases using a model system. | 12-25-2014 |
| 20150037866 | Composition, Method and Kit for Obtaining Purified Recombinant Proteins - Compositions relating to a combination of two types of separation matrix; and to variant host cells which contain at least one essential host protein that is fused to an affinity binding tag or has been mutated to replace at least two of a plurality of histidines or basic amino acids are provided. Methods are also provided that relate to isolating a recombinant protein from a lysate. | 02-05-2015 |
| 20150037867 | PURIFICATION METHOD - The invention provides methods for purifying protein products. | 02-05-2015 |
| 20150044753 | Expression Vector Comprising a Polynucleotide Encoding a Modified Glutamine Synthetase and a Method for Preparing a Target Protein Employing the Same - The present invention relates to a vector comprising a polynucleotide encoding a modified glutamine synthetase (GS), and a method for preparing a target protein employing the same. More particularly, the present invention relates to a modified GS having an increased sensitivity to a glutamine synthetase (GS) inhibitor, a polynucleotide encoding the modified GS, a vector comprising the polynucleotide, a transformant comprising the vector, and a method for preparing a target protein using the transformant. | 02-12-2015 |
| 20150072395 | RECOMBINANT MICROORGANISMS AND METHODS OF USE THEREOF - The present provides selection markers, methods, nucleic acids, and vectors of use in the preparation of recombinant | 03-12-2015 |
| 20150093799 | HISTIDYL-TRNA SYNTHETASES FOR TREATING AUTOIMMUNE AND INFLAMMATORY DISEASES - The present invention relates generally to compositions and methods comprising histidyl-tRNA synthetase polypeptides or other specific blocking agents for the treatment autoimmune diseases and other inflammatory diseases, including those related to Jo-1 antibodies. | 04-02-2015 |
| 20150307526 | Guanidine-Functionalized Particles and Methods of Making and Using - Guanidine-functionalized particles and methods of making and using such particles. | 10-29-2015 |
| 20150353896 | Seed Train Processes and Uses Thereof - Provided herein are seed train processes and methods of producing a recombinant protein that include the use of these seed train processes. | 12-10-2015 |
| 20160108106 | GENERATION OF HIGHLY POTENT ANTIBODIES NEUTRALIZING THE LUKGH (LUKAB) TOXIN OF STAPHYLOCOCCUS AUREUS - The subject relates to an isolated | 04-21-2016 |
| 20160152662 | LIGAND FUNCTIONAL SUBSTRATES | 06-02-2016 |
