| Entries |
| Document | Title | Date |
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| 20080248540 | METHODS OF PRODUCING BUTANOL - A method of producing butanol from carbohydrates is provided. A feedstock comprising a carbohydrate source is fermented in the presence of bacteria to produce butyric acid and hydrogen. The butyric acid is then hydrogenated in the presence of a catalyst to produce butanol. | 10-09-2008 |
| 20080274524 | METHOD FOR THE PRODUCTION OF 1-BUTANOL - A method for the production of 1-butanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product. | 11-06-2008 |
| 20080274525 | METHOD FOR THE PRODUCTION OF 2-BUTANOL - A method for the production of 2-butanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product. | 11-06-2008 |
| 20080274526 | METHOD FOR THE PRODUCTION OF ISOBUTANOL - A method for the production of isobutanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product. | 11-06-2008 |
| 20090081746 | BIOFUEL PRODUCTION BY RECOMBINANT MICROORGANISMS - Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from a suitable substrate. | 03-26-2009 |
| 20090093035 | Blocking Sporulation by Inhibiting SPOIIE - We have shown that the control of solventogenesis and sporulation can be genetically uncoupled in | 04-09-2009 |
| 20090111154 | BUTANOL PRODUCTION BY RECOMBINANT MICROORGANISMS - Provided are microorganisms that catalyze the synthesis of biofuels from a suitable substrate such as glucose. Also provided are methods of generating such organisms and methods of synthesizing biofuels using such organisms. Provided are microorganisms comprising non-naturally occurring metabolic pathway for the production of higher alcohols. | 04-30-2009 |
| 20090137016 | Process for rapid anaerobic digestion of biomass using microbes and the production of biofuels therefrom - A process for effecting anaerobic digestion of plant biomass, which entails the step of anaerobically digesting plant biomass with at least one species of rumen microorganisms. | 05-28-2009 |
| 20090155869 | ENGINEERED MICROORGANISMS FOR PRODUCING N-BUTANOL AND RELATED METHODS - A recombinant microorganism expressing at least a heterologous enzyme of an NADH-dependent pathway for conversion of a carbon source to n-butanol, metabolic intermediate and/or a derivative thereof and capable of producing n-butanol, a metabolic intermediate and/or a derivative thereof at a high yield and related methods. The recombinant microorganism engineered to inactivate a native enzyme of one or more pathways that compete with NADH-dependent heterologous pathway, and/or to balance the NADH-dependent heterologous pathway with respect to NADH production and consumption. | 06-18-2009 |
| 20090155870 | FERMENTIVE PRODUCTION OF FOUR CARBON ALCOHOLS - Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein. Specifically disclosed herein are the use of coenzyme B | 06-18-2009 |
| 20090176288 | Methods and Compositions for Butanol Production - A butanol producing cell and a method for the production of butanol are provided. Butanol producing cells comprising a butanol synthesis system or butanol export proteins may be used to enhance the production of butanol from a carbon substrate composition. | 07-09-2009 |
| 20090203097 | STRAIN FOR BUTANOL PRODUCTION - Screening of fatty acid fed bacteria which are not natural butanol producers identified increased membrane cyclopropane fatty acid as providing improved butanol tolerance. Increasing expression of cyclopropane fatty acid synthase in the presence of the enzyme substrate that is either endogenous to the cell or fed to the cell, increased butanol tolerance. Bacterial strains with increased cyclopropane fatty acid synthase and having a butanol biosynthetic pathway are useful for production of butanol. | 08-13-2009 |
| 20090203098 | INDIRECT PRODUCTION OF BUTANOL AND HEXANOL - A process for the conversion of carbohydrates from any of a number of sources into butanol and hexanol for fuel or chemical use is disclosed. The process includes conducting a homoacetogenic fermentation to produce an acetic acid intermediate which is chemically converted to ethanol. The ethanol and a remaining portion of the acetic acid intermediate are used as a substrate in an acidogenic fermentation to produce butyric and caproic acid intermediates which are then chemically converted to butanol and hexanol. | 08-13-2009 |
| 20090215137 | METHODS FOR THE ECONOMICAL PRODUCTION OF BIOFUEL PRECURSOR THAT IS ALSO A BIOFUEL FROM BIOMASS - Methods for producing a biofuel precursor are provided. Also provided are biocatalysts that convert a feedstock to a biofuel precursor. | 08-27-2009 |
| 20090221047 | WEB COMPRISING FINE FIBER AND BIOACTIVE PARTICULATE AND USES THEREOF - The assemblies of the invention can comprise a fine fiber layer forming a multilamellar web or matrix, having dispersed within the fine fiber layer a bioactive particulate material, including cells, enzymes or microorganisms. Fluid that flows through the assemblies of the invention can have any material dispersed or dissolved in the fluid react with, be absorbed by, or adsorbed onto, the bioactive particulate within the nanofiber layer. The assemblies of the invention can be used to treat or purify fluid streams. The assemblies of the invention can be used in conjunction with a bioreactor system, a bioartificial organ, or a culture container. | 09-03-2009 |
| 20090239275 | FERMENTIVE PRODUCTION OF FOUR CARBON ALCOHOLS - Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein. | 09-24-2009 |
| 20090269823 | BUTANOL DEHYDROGENASE ENZYME FROM THE BACTERIUM ACHROMOBACTER XYLOSOXIDANS - From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of | 10-29-2009 |
| 20090275097 | PRIMARY ALCOHOL PRODUCING ORGANISMS - The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms. | 11-05-2009 |
| 20090280546 | YEAST STRAIN FOR PRODUCTION OF FOUR CARBON ALCOHOLS - Yeast cells with a reduced general control response to amino acid starvation were found to have increased tolerance to butanol in the growth medium. The reduced response was engineered by genetic modification of a gene involved in the response, a GCN gene, to eliminate activity of the encoded protein. Yeast strains with an engineered butanol biosynthetic pathway and a genetic modification in a gene involved in the general control response to amino acid starvation, which have increased butanol tolerance, are useful for production of butanol. | 11-12-2009 |
| 20090305369 | DELETION MUTANTS FOR THE PRODUCTION OF ISOBUTANOL | 12-10-2009 |
| 20090305370 | METHOD FOR PRODUCING BUTANOL USING TWO-PHASE EXTRACTIVE FERMENTATION - A method of making butanol from at least one fermentable carbon source that overcomes the issues of toxicity resulting in an increase in the effective titer, the effective rate, and the effective yield of butanol production by fermentation utilizing a recombinant microbial host wherein the butanol is extracted into specific organic extractants during fermentation | 12-10-2009 |
| 20100062505 | BUTANOL PRODUCTION BY METABOLICALLY ENGINEERED YEAST - There are disclosed metabolically-engineered yeast and methods of producing n-butanol. In an embodiment, metabolically-engineered yeast is capable of metabolizing a carbon source to produce n-butanol, at least one pathway produces increased cytosolic acetyl-CoA relative to cytosolic acetyl-CoA produced by a wild-type yeast, and at least one heterologous gene encodes and expresses at least one enzyme for a metabolic pathway capable of utilizing NADH to convert acetyl-CoA to n-butanol. In another embodiment, a method of producing n-butanol includes (a) providing metabolically-engineered yeast capable of metabolizing a carbon source to produce n-butanol, at least one pathway produces increased cytosolic acetyl-CoA relative to cytosolic acetyl-CoA produced by a wild-type yeast, and at least one heterologous gene encodes and expresses at least one enzyme for a metabolic pathway utilizing NADH to convert acetyl-CoA to n-butanol; and (b) culturing the yeast to produce n-butanol. Other embodiments are also disclosed. | 03-11-2010 |
| 20100081182 | ENHANCED IRON-SULFUR CLUSTER FORMATION FOR INCREASED DIHYDROXY-ACID DEHYDRATASE ACTIVITY IN LACTIC ACID BACTERIA - Lactic acid bacteria expressing dihydroxyacid dehydratase polypeptides with increased specific activity are disclosed. The lactic acid bacteria comprise recombinant genes encoding iron-sulfur cluster forming proteins. | 04-01-2010 |
| 20100081183 | ENHANCED DIHYDROXY-ACID DEHYDRATASE ACTIVITY IN LACTIC ACID BACTERIA - Lactic acid bacterial (LAB) cells were modified such that they have a specific activity of dihydroxy-acid dehydratase enzyme activity that is increased to about 0.1 μmol min | 04-01-2010 |
| 20100086982 | PROCESS FOR THE BIOLOGICAL PRODUCTION OF n-BUTANOL WITH HIGH YIELD - The present invention provides a method for the biological production of n-butanol at high yield from a fermentable carbon source. In one aspect of the present invention, a process for the conversion of glucose to n-butanol is achieved by the use of a recombinant organism comprising a host | 04-08-2010 |
| 20100112655 | ENHANCED PYRUVATE TO 2,3-BUTANEDIOL CONVERSION IN LACTIC ACID BACTERIA - A high flux of metabolites from pyruvate to 2,3-butanediol in | 05-06-2010 |
| 20100120106 | Process for producing lower alkyl alcohols from cellulosic biomass using microorganisms - At least one isolated microorganism, which converts at least 10% by weight, and preferably 50% by weight, of cellulosic biomass to a lower alkyl alcohol by direct digestion, and which produces at least 4% by volume of the lower alkyl alcohol in an aqueous-based digestion medium. | 05-13-2010 |
| 20100120107 | PRODUCTION OF BIOFUEL AND PROTEIN FROM A RAW MATERIAL - The present invention relates to a method for providing an isolated biofuel and a purified protein product from a raw material suitable for the production of the biofuel or a derivative of said raw material. The method comprises the steps of: (i) subjecting the raw material or a derivative of said raw material to at least one first treatment liberating the biofuel from the raw material or the derivative of said raw material, (ii) isolating the biofuel liberated in step (i) obtaining the isolated biofuel, (iii) subjecting the raw material or a derivative of said raw material to at least one second treatment providing a material suspension, and (iv) subjecting the material suspension from step (iii) to an expanded bed adsorption process obtaining the purified protein product. | 05-13-2010 |
| 20100129885 | Methods for the production of n-butanol - Embodiments of the present invention include methods for the production of four carbon alcohols, specifically n-butanol, by a consolidated bioprocessing approach for the conversion of cellulosic material to the desired end product. According to some embodiments, recombinant microbial host cells are provided, preferably | 05-27-2010 |
| 20100129886 | PRODUCTION OF ISOBUTANOL IN YEAST MITOCHONDRIA - Yeast cells with modified expression of certain enzyme activities in the mitochondria are described for isobutanol production. Modifications described provide an isobutanol biosynthesis pathway in the yeast mitochondria. | 05-27-2010 |
| 20100129887 | INCREASED PRODUCTION OF ISOBUTANOL IN YEAST WITH REDUCED MITOCHONDRIAL AMINO ACID BIOSYNTHESIS - Yeast cells with reduced activity of certain enzymes involved in branched chain amino acid biosynthesis in yeast mitochondria are described. Target enzymes include threonine deaminase, isopropylmalate synthase, and optionally branched chain amino acid transaminase. | 05-27-2010 |
| 20100136640 | ENHANCED BUTANOL PRODUCING MICROORGANISMS AND METHOD FOR PREPARING BUTANOL USING THE SAME - The present invention relates to a recombinant mutant microorganism having enhanced butanol producing capacity and a method for producing butanol using the same. In the microorganism, genes coding for enzymes responsible for the biosynthesis of lactate, ethanol and/or acetate are deleted or attenuated and genes coding for enzymes involved in butanol biosynthesis are introduced and amplified. | 06-03-2010 |
| 20100136641 | STRAIN FOR BUTANOL PRODUCTION WITH INCREASED MEMBRANE UNSATURATED TRANS FATTY ACIDS - Bacteria that are not natural butanol producers were found to have increased tolerance to butanol when the membrane content of unsaturated trans fatty acids was increased. Feeding cells with unsaturated trans fatty acids increased their concentration in the membrane, which may also be accomplished by expressing a fatty acid cistrans isomerase. | 06-03-2010 |
| 20100143992 | Process for Fermentive Preparation of Alcohols and Recovery of Product - This invention relates to a process for recovering an alcohol from a fermentation broth using liquid-liquid extraction, wherein at least one ionic liquid is used as the extractive solvent. | 06-10-2010 |
| 20100143993 | PROCESS FOR FERMENTIVE PREPARATIONFOR ALCOLHOLS AND RECOVERY OF PRODUCT - This invention relates to a process for recovering an alcohol from a fermentation broth using liquid-liquid extraction, wherein at least one ionic liquid is used as the extractive solvent. | 06-10-2010 |
| 20100143994 | PROCESS FOR FERMENTIVE PREPARATION OF ALCOHOLS AND RECOVERY OF PRODUCT - This invention relates to a process for recovering an alcohol from a fermentation broth using liquid-liquid extraction, wherein at least one ionic liquid is used as the extractive solvent. | 06-10-2010 |
| 20100143995 | Process for Fermentive Preparation of Alcohols and Recovery of Product - This invention relates to a process for recovering an alcohol from a fermentation broth using liquid-liquid extraction, wherein at least one ionic liquid is used as the extractive solvent. | 06-10-2010 |
| 20100143996 | Continuous Single Vessel Butanol Synthesis By Fermentation - The present invention describes a method for producing butanol by fermentation of carbohydrates using mixed populations of acidogenic-phase cells and solventogenic-phase cells of | 06-10-2010 |
| 20100143997 | ENGINEERED MICROORGANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS - The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention. | 06-10-2010 |
| 20100143998 | SYSTEMS AND METHODS FOR PRODUCING BIOFUELS AND RELATED MATERIALS - cells (American Type Culture Collection 700394 | 06-10-2010 |
| 20100151543 | RECOMBINANT MICROORGANISMS HAVING MODIFIED PRODUCTION OF ALCOHOLS AND ACIDS - Recombinant acetogenic Clostridia are engineered to modulate production of aliphatic C | 06-17-2010 |
| 20100151544 | SYSTEMS AND METHODS FOR SELECTIVE ALCOHOL PRODUCTION - The present invention relates to metabolic engineering issues related to flux determinism in core primary-metabolism pathways. In particular, the present invention relates to alcohol (e.g., butanol) production and selectivity, and related methods thereof. | 06-17-2010 |
| 20100151545 | PRODUCTION OF BRANCHED-CHAIN ALCOHOLS BY PHOTOSYNTHETIC MICROORGANISMS - The present invention provides genes, polypeptides and expression constructs therefor, recombinant photosynthetic microorganisms, and method of use thereof, such as for the production of branched-chain alcohols (including 2-methyl-1-butanol, 3-methyl-1-butanol, and isobutanol) and derivatives thereof for a variety of uses. | 06-17-2010 |
| 20100151546 | SYSTEMS AND METHODS FOR PRODUCING BIOFUELS AND RELATED MATERIALS - cells (American Type Culture Collection 700394 | 06-17-2010 |
| 20100159546 | METABOLIC ENGINEERING OF YEASTS FOR THE PRODUCTION OF 1-BUTANOL - The present invention provides genetically modified yeast cells, and methods of using those yeast cells, to produce 1-butanol. The yeast cell can be selected from the genera | 06-24-2010 |
| 20100167363 | YEAST WITH INCREASED BUTANOL TOLERANCE INVOLVING FILAMENTOUS GROWTH RESPONSE - Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the filamentous growth response. Yeast with increased expression of MSS11 | 07-01-2010 |
| 20100167364 | YEAST WITH INCREASED BUTANOL TOLERANCE INVOLVING CELL WALL INTEGRITY PATHWAY - Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the cell wall integrity pathway. Yeast with increased expression of SLT2p, a mitogen activated protein kinase of the MAPK module of the cell wall integrity pathway had increased tolerance to isobutanol. These yeast may be used for improved butanol production. | 07-01-2010 |
| 20100167365 | YEAST WITH INCREASED BUTANOL TOLERANCE INVOLVING HIGH OSMOLARITY/GLYCEROL RESPONSE PATHWAY - Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the high osmolarity/glycerol response pathway. Yeast with increased expression of PBS2p, a mitogen activated protein kinase kinase of the MAPK module of the high osmolarity/glycerol response pathway may be used for improved butanol production. | 07-01-2010 |
| 20100209986 | BIOFUEL PRODUCTION BY RECOMBINANT MICROORGANISMS - Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from a suitable substrate. | 08-19-2010 |
| 20100216200 | SYSTEMS AND METHODS FOR PRODUCING BIOFUELS AND RELATED MATERIALS - cells (American Type Culture Collection 700394 | 08-26-2010 |
| 20100221801 | YEAST WITH INCREASED BUTANOL TOLERANCE INVOLVING A MULTIDRUG EFFLUX PUMP GENE - Increasing tolerance to butanol in yeast has been accomplished by decreasing activity of Pdr5p encoded by an endogenous PDR5 gene. A deletion mutation of the PDR5 gene led to improved growth yield in the presence of butanol. Yeast cells with reduced Pdr5p activity, or other multidrug resistance ATP-binding cassette transporter protein activity encoded by CDR1 or BFR1, and a butanol biosynthetic pathway may be used for improved butanol production | 09-02-2010 |
| 20100221802 | METHOD FOR PRODUCING BUTANOL USING TWO-PHASE EXTRACTIVE FERMENTATION - A method of making butanol from at least one fermentable carbon source that overcomes the issues of toxicity resulting in an increase in the effective titer, the effective rate, and the effective yield of butanol production by fermentation utilizing a recombinant microbial host wherein the butanol is extracted into specific organic extractants during fermentation | 09-02-2010 |
| 20100255552 | Butanal Production Using Engineered Streptomyces Coelicolor - A method of producing butanal by optimizing the growth of | 10-07-2010 |
| 20100261241 | METHODS FOR THE PRODUCTION OF N-BUTANOL - Embodiments of the present invention include methods for the production of four carbon alcohols, specifically n-butanol, by a consolidated bioprocessing approach for the conversion of cellulosic material to the desired end product. According to some embodiments, recombinant microbial host cells are provided, preferably | 10-14-2010 |
| 20100279370 | METHOD FOR CONTROLLING BUTANOL CONCENTRATION IN FERMENTATION BROTH - The present invention relates to a two stage process to control the butanol concentration from fermentation broth. | 11-04-2010 |
| 20100285549 | RECOMBINANT MICROORGANISM HAVING BUTANOL PRODUCTION CAPACITY AND BUTANOL PRODUCTION METHOD - This invention relates to a recombinant microorganism having improved butanol production capacity and butanol production with the use of such recombinant microorganism with good efficiency. In this invention, the acetoacetyl-CoA synthase gene encoding an enzyme capable of synthesizing acetoacetyl-CoA from malonyl-CoA and acetyl-CoA and a group of genes involved in butanol biosynthesis that enables synthesis of butanol from acetoacetyl-CoA are introduced into a host microorganism. | 11-11-2010 |
| 20100285550 | Delignification of Lignocellulose-Containing Material - The invention relates to processes of delignifying lignocellulose-containing material, wherein the lignocellulose-containing material is treated with a delignification catalyst and a lignin solubilizing agent. | 11-11-2010 |
| 20100291646 | Process for the Recovery of a Fermentation Product - The present invention provides a process for the purification and concentration of a partially water-soluble organic product from a source containing the same at concentration C0, which process comprises the steps of: (i) concentrating said product by means of a resin to form a first aqueous solution at concentration of C1; and (ii) fractionating an aqueous solution by temperature adjustment to form a second aqueous solution and a third aqueous solution with concentrations C2 and C3, respectively, wherein C2>C3 and C2>C1>C0. | 11-18-2010 |
| 20100291647 | Microorganisms Having Enhanced Resistance To Acetate And Related Compositions And Methods of Use - The present invention provides isolated or genetically modified strains of microorganisms that display enhanced resistance to acetate as a result of increased expression of a sodium proton antiporter. The present invention also provides methods for producing such microbial strains, as well as related promoter sequences and expression vectors. Further, the present invention provides methods of producing alcohol from biomass materials by using microorganisms with enhanced resistance to acetate. | 11-18-2010 |
| 20100297717 | METHOD OF PRODUCING ALCOHOL IN THE BIOREFINERY CONTEXT - The present invention describes a method of producing alcohol from pretreated lignocellulosic biomass wherein the enzymatic hydrolysis stage is carried out with cellulolytic and/or hemicellulolytic enzymes produced using at least one effluent from another ethanol production process using a sugar plant as the feedstock. This method can be integrated in a method of producing alcohol from cellulosic or lignocellulosic materials, referred to as second-generation method, comprising the following stages:
| 11-25-2010 |
| 20100297718 | Processes of Producing Fermentation Products - The invention relates to processes of fermenting plant material into a fermentation product using a fermenting organism, wherein one or more compounds capable of activating a sirtuin protein is present during fermentation. | 11-25-2010 |
| 20100323418 | ORGANISMS FOR THE PRODUCTION OF ISOPROPANOL, n-BUTANOL, AND ISOBUTANOL - A non-naturally occurring microbial organism having an isopropanol pathway includes at least one exogenous nucleic acid encoding an isopropanol pathway enzyme. The pathway includes an enzyme selected from a 4-hydroxybutyryl-CoA dehydratase, a crotonase, a 3-hydroxybutyryl-CoA dehydrogenase, an acetoacetyl-CoA synthetase, an acetyl-CoA:acetoacetate-CoA transferase, an acetoacetyl-CoA hydrolase, an acetoacetate decarboxylase, and an acetone reductase. A non-naturally occurring microbial organism having an n-butanol pathway includes at least one exogenous nucleic acid encoding an n-butanol pathway enzyme. Other non-naturally occurring microbial organism have n-butanol or isobutanol pathways. The organisms are cultured to produce isopropanol, n-butanol, or isobutanol. | 12-23-2010 |
| 20100330636 | PROCESS FOR THE BIOLOGICAL PRODUCTION OF N-BUTANOL WITH HIGH YIELD - The present invention provides a method for the biological production of n-butanol at high yield from a fermentable carbon source. In one aspect of the present invention, a process for the conversion of glucose to n-butanol is achieved by the use of a recombinant organism comprising a host | 12-30-2010 |
| 20100330637 | Designer Organisms for Photobiological Butanol Production from Carbon Dioxide and Water - The present invention provides a biosafety-guarded photobiological butanol production technology based on designer transgenic plants, designer algae, designer blue-green algae (cyanobacteria and oxychlorobacteria), or designer plant cells. The designer photosynthetic organisms are created such that the endogenous photobiological regulation mechanism is tamed, and the reducing power (NADPH) and energy (ATP) acquired from the photosynthetic process are used for synthesis of butanol (CH | 12-30-2010 |
| 20110020888 | METHOD FOR PREPARING BUTANOL THROUGH BUTYRYL-CoA AS AN INTERMEDIATE USING BACTERIA - The present invention relates to a method for producing butanol using a bacterium capable of biosynthesizing butanol from butyryl-CoA as an intermediate. More particularly, a method for producing butanol, the method comprising generating bytyryl-CoA in a bacterium which contains a gene coding for AdhE (an enzyme responsible for the conversion of butyryl-CoA to butanol) using various methods, and converting the butyryl-CoA into butanol. | 01-27-2011 |
| 20110020889 | YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD - The present invention provides recombinant mircoorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 01-27-2011 |
| 20110027845 | ENHANCED ETHANOL AND BUTANOL PRODUCING MICROORGANISMS AND METHOD FOR PREPARING ETHANOL AND BUTANOL USING THE SAME - The present invention relates to a recombinant microorganism having an enhanced ability to produce ethanol and butanol and a method for preparing ethanol and butanol using the same, and more particularly to a recombinant microorganism having an enhanced ability to produce ethanol and butanol, into which a gene encoding CoA transferase and a gene encoding alcohol/aldehyde dehydrogenase are introduced, and to a method for preparing ethanol and butanol using the same. The recombinant microorganism according to the present invention, obtained by manipulating metabolic pathways of microorganisms, is capable of producing butanol and ethanol exclusively without producing any byproduct, and thus is useful as a microorganism producing industrial solvents and transportation fuel. | 02-03-2011 |
| 20110053235 | FERMENTATIVE PRODUCTION OF ISOBUTANOL WITH YEAST - The invention relates to a yeast cell producing isobutanol, characterized in that the cell has an increased metabolic flow of material from pyruvate and acetolactate, 2,3-dihydroxy isovalerate, 2-ketoisovalerate, isobutyraldehyde to isobutanol, in that at least one of the genes coding the enzymes, which are involved in this conversion, is over-expressed, and without any of said genes being heterologous to said yeast cell, and to a method for the production of isobutanol using yeast cells, comprising the provision of the yeast cells according to the invention, and bringing the yeast cell into contact with a fermentable carbon source. | 03-03-2011 |
| 20110065157 | PHOTOBIOREACTOR FOR ALGAE GROWTH - The present invention provides a flow-through photobioreactor containing at least one thermoplastic multi-wall sheet having an upper layer and a lower layer having arranged there between at least two sidewalls, at least one inner wall and two or more end caps. Also provided is a process for the production of a biofuel with the inventive photobioreactor. The photobioreactor and process of the present invention have the following advantages: genetically engineered microbes that give higher yields cannot escape into the environment, water in the system does not evaporate, no weeding (presence of unwanted algae), UV light from the sun is filtered out by the reactor walls, temperature control is possible, CO | 03-17-2011 |
| 20110076732 | Process, Plant, and Butanol From Lignocellulosic Feedstock - This invention relates to a process, a plant, and butanol made of or derived from lignocellulosic feedstock. The process includes the step of depolymerizing lignocellulosic material to form pentose and a remainder. The process also includes the step of converting the pentose to butanol material and using the remainder for generation of power or further downstream conversion. | 03-31-2011 |
| 20110076733 | CYTOSOLIC ISOBUTANOL PATHWAY LOCALIZATION FOR THE PRODUCTION OF ISOBUTANOL - The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and/or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 03-31-2011 |
| 20110097773 | METHOD FOR PRODUCING BUTANOL USING EXTRACTIVE FERMENTATION - A method for producing butanol through microbial fermentation, in which the butanol product is removed by extraction into a water-immiscible extractant composition comprising a first solvent and a second solvent, is provided. The first solvent is selected from the group consisting of C | 04-28-2011 |
| 20110097774 | VISIBLE LIGHT-ENHANCED ENZYMATIC PROMOTION OF HYDROCARBON REACTIONS - A photocatalytic method for the hydroxylation of alkanes involving the use of a Cytochrome P450 class of enzyme as a catalyst which is activated by exposure to a pulsed blue light emitted from a light emitting diode (LED) at 450 nm±25 nm. | 04-28-2011 |
| 20110097775 | PRODUCTION OF BUTANOL - The present invention relates to methods for making recombinant thermophilic bacteria of the family Bacillaceae. In particular, recombinant thermophilic Bacillaceae are provided which have been engineered to produce butanol and/or butyrate. Preferably, heterologous nucleic acid molecules encoding one or more butanol or butyrate biosynthetic pathway enzymes are introduced into a thermophilic Bacillaceae in order to produce a recombinant thermophilic Bacillaceae which is capable of producing butanol. The Bacillaceae is preferably of the genus | 04-28-2011 |
| 20110111472 | FERMENTIVE PRODUCTION OF FOUR CARBON ALCOHOLS - Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway. | 05-12-2011 |
| 20110129887 | IMMOBILIZED PRODUCT TOLERANT MICROORGANISMS - Methods for adapting or selecting microorganisms with increased product tolerance are provided Additionally, a bioreactor capable of operation in either packed bed or fluidized bed is disclosed along with methods to use the bioreactor for culturing microorganisms adapted or selected increased product tolerance | 06-02-2011 |
| 20110136192 | FLUX TO ACETOLACTATE-DERIVED PRODUCTS IN LACTIC ACID BACTERIA - An engineering method was developed to allow genetic modification and isolation of lactic acid bacteria cells that lack lactate dehydrogenase and acetolactate decarboxylase activities. In cells with these modifications and an isobutanol biosynthetic pathway, improved production of isobutanol was observed. | 06-09-2011 |
| 20110136193 | METHOD FOR PRODUCING BUTANOL USING EXTRACTIVE FERMENTATION WITH OSMOLYTE ADDITION - A method is provided for producing butanol through microbial fermentation, in which the butanol product is removed during the fermentation by extraction into a water-immiscible organic extractant in the presence of at least one osmolyte at a concentration at least sufficient to increase the butanol partition coefficient relative to that in the presence of the osmolyte concentration of the basal fermentation medium and of an optional fermentable carbon source. The osmolyte may comprise a monosaccharide, a disaccharide, glycerol, sugarcane juice, molasses, polyethylene glycol, dextran, high fructose corn syrup, corn mash, starch, cellulose, and combinations thereof. Also provided is a method and composition for recovering butanol from a fermentation medium. | 06-09-2011 |
| 20110143407 | Continuous Single Vessel Butanol Synthesis by Fermentation - The present invention describes a method for producing butanol by fermentation of carbohydrates using mixed populations of acidogenic-phase cells and solventogenic-phase cells of | 06-16-2011 |
| 20110159558 | METHOD FOR PRODUCING BUTANOL USING EXTRACTIVE FERMENTATION WITH ELECTROLYTE ADDITION - A method for producing butanol through microbial fermentation, in which the butanol product is removed during the fermentation by extraction into a water-immiscible organic extractant in the presence of at least one electrolyte at a concentration at least sufficient to increase the butanol partition coefficient relative to that in the presence of the salt concentration of the basal fermentation medium, is provided. The electrolyte may comprise a salt which dissociates in the fermentation medium, or in the aqueous phase of a biphasic fermentation medium, to form free ions. Also provided is a method and composition for recovering butanol from a fermentation medium. | 06-30-2011 |
| 20110159559 | METHOD FOR THE COMBINED PRODUCTION OF BUTANOL AND HYDROGEN - The invention relates to a process for the combined production of butanol and hydrogen from biomass, comprising the steps of fermenting biomass to obtain butanol in a first reaction mixture; removing the butanol and hydrogen from the first reaction mixture to obtain effluent; and using the effluent as a substrate in a second reaction mixture in a process using low substrate concentrations, in particular a hydrogen production process. Preferably, the process using low substrate concentrations is a hydrogen production process and at least part of the end products of the hydrogen production process is removed from the second reaction mixture for obtaining an effluent that comprises organic acid, which effluent is returned to the first reaction mixture. | 06-30-2011 |
| 20110171706 | Integrated Process for Production of Alcohol from Syngas and Removal of CO2 - A process for producing alcohol from syngas integrates the alcohol recovery with the removal of CO | 07-14-2011 |
| 20110177571 | DESIGNER CALVIN-CYCLE-CHANNELED PRODUCTION OF BUTANOL AND RELATED HIGHER ALCOHOLS - Designer Calvin-cycle-channeled and photosynthetic NADPH-enhanced pathways, the associated designer genes and designer transgenic photosynthetic organisms for photobiological production of butanol and related higher alcohols from carbon dioxide and water are provided. The butanol and related higher alcohols include 1-butanol, 2-methyl-1-butanol, isobutanol, 3-methyl-1-butanol, 1-hexanol, 1-octanol, 1-pentanol, 1-heptanol, 3-methyl-1-pentanol, 4-methyl-1-hexanol, 5-methyl-1-heptanol, 4-methyl-1-pentanol, 5-methyl-1-hexanol, and 6-methyl-1-heptanol. The designer photosynthetic organisms such as designer transgenic oxyphotobacteria and algae comprise designer Calvin-cycle-channeled and photosynthetic NADPH-enhanced pathway gene(s) and biosafety-guarding technology for enhanced photobiological production of butanol and related higher alcohols from carbon dioxide and water. | 07-21-2011 |
| 20110183392 | YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD - There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical. | 07-28-2011 |
| 20110183393 | METHODS OF INCREASING DIHYDROXY ACID DEHYDRATASE ACTIVITY TO IMPROVE PRODUCTION OF FUELS, CHEMICALS, AND AMINO ACIDS - The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 07-28-2011 |
| 20110201072 | Modified alcohol dehydrogenases for the production of fuels and chemicals - The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 08-18-2011 |
| 20110201073 | Reduced by-product accumulation for improved production of isobutanol - The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 08-18-2011 |
| 20110207191 | METHOD FOR PRODUCING ALCOHOL USING BIOETHANOL WASTE FERMENTED SOLUTION - Provided is a method for producing alcohol or hydrogen gas comprising culturing alcohol-producing microorganisms in a waste fermented solution generated from the bioethanol production process. Glycerol included in the waste fermented solution generated from the bioethanol production process is converted by the microorganisms to butanol under an anaerobic condition. Since the waste fermented solution generated from the bioethanol production process can be utilized as a source of a biofuel, environmental and energy problems can be solved at once. | 08-25-2011 |
| 20110236941 | RECOMBINANT MICROORGANISM AND METHODS OF PRODUCTION THEREOF - A novel genetically modified microorganisms capable of using CO to produce 1-butanol and/or a precursor thereof, novel methyltransferases and nucleic acids encoding same, methods for producing genetically modified microorganisms using said novel methyltransferases, and methods of producing 1-butanol and/or a precursor thereof by microbial fermentation. | 09-29-2011 |
| 20110236942 | Reduced by-product accumulation for improved production of isobutanol - The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 09-29-2011 |
| 20110262982 | ACETOLACTATE SYNTHASE HAVING 2-KETOISOVALERATE DECARBOXYLASE ACTIVITY AND USES THEREOF - Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and 2-phenylethanol from a suitable substrate and a recombinant acetolactate synthase having both synthase and decarboxylase activity. | 10-27-2011 |
| 20110269199 | ALCOHOL DEHYDROGENASES (ADH) USEFUL FOR FERMENTIVE PRODUCTION OF LOWER ALKYL ALCOHOLS - The invention relates to suitable candidate ADH enzymes for production of lower alkyl alcohols including isobutanol. The invention also relates to recombinant host cells that comprise such ADH enzymes and methods for producing lower alkyl alcohols in the same. | 11-03-2011 |
| 20110275129 | Reduced by-product accumulation for improved production of isobutanol - The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 11-10-2011 |
| 20110281314 | METHODS, SYSTEMS AND COMPOSITIONS RELATED TO MICROBIAL BIO-PRODUCTION OF BUTANOL AND/OR ISOBUTANOL - Embodiments herein generally relate to methods, compositions, systems and uses for enabling bio-production of or increasing bio-production of alcohol molecules by microorganisms. Certain embodiments relate to compositions and methods enabling or increasing the bio-production of 4-carbon alcohol molecules by bacteria. In some embodiments, compositions and methods relate to introducing isobutyryl-CoA isomerase to a culture of microorganisms to enable or increase the bio-production of four-carbon alcohols. Variations of biosynthesis pathways for microbial bio-production of butanol and/or isobutanol are provided. | 11-17-2011 |
| 20110281315 | METHOD FOR PRODUCING 2-BUTANOL AND RECOMBINANT MICROORGANISM HAVING 2-BUTANOL PRODUCTION CAPACITY - This invention is intended to produce 2-butanol with excellent productivity via a fermentation process. Recombinant microorganisms into which the acetoacetyl-CoA synthase gene and a group of genes (i.e., genes involved in 2-propanol synthesis) encoding a set of enzymes synthesizing 2-propanol from acetoacetyl-CoA have been introduced are cultured, so that, in addition to 2-propanol, 2-butanol is produced at a high level in a medium. | 11-17-2011 |
| 20110287500 | CYTOSOLIC ISOBUTANOL PATHWAY LOCALIZATION FOR THE PRODUCTION OF ISOBUTANOL - The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and/or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 11-24-2011 |
| 20110294179 | METHOD FOR PRODUCING BUTANOL USING TWO-PHASE EXTRACTIVE FERMENTATION - A method of making butanol from at least one fermentable carbon source that overcomes the issues of toxicity resulting in an increase in the effective titer, the effective rate, and the effective yield of butanol production by fermentation utilizing a recombinant microbial host wherein the butanol is extracted into specific organic extractants during fermentation | 12-01-2011 |
| 20110300596 | PREPARATION METHOD FOR ALCOHOL FROM CARBOXYLIC ACID BY ONE-STEP PROCESS - The present invention relates to a preparation method for alcohol by reacting carboxylic acid, alcohol, and hydrogen using hydrogenation catalysts. More specifically, the invention relates to a method for preparing alcohol by performing esterification and hydrocracking in a one-step process using hydrogenation catalysts instead of a two-step process. According to the invention, alcohol is prepared from carboxylic acid through esterification and hydrogenation in a one-step process using hydrogenation catalysts. Therefore, production costs and by-product treatment costs can be reduced in comparison to a two-step process. In addition, the invention is effective and economical since it can produce alcohol at relatively high yield by a simple process. Further, the invention allows high yield at relatively lower pressure when compared to alcohol production from carboxylic acid through hydrogenation without esterification and solves the problems of leaching by catalysts. | 12-08-2011 |
| 20110312052 | PROCESS FOR CELL-FREE PRODUCTION OF CHEMICALS - An enzymatic process is described for the production of chemicals from carbon sources. In particular, according to one aspect, a process for the production of a target organic compound from a carbon source by a cell-free enzyme system is disclosed. | 12-22-2011 |
| 20110312053 | SUPPLEMENTATION OF FATTY ACIDS FOR IMPROVING ALCOHOL PRODUCTIVITY - Fatty acids derived from biomass at a step in a fermentation process can be added to a fermentation medium comprising a recombinant microorganism that produces a product alcohol. At least one of growth rate and fermentable carbon consumption of the microorganism is greater in the presence of the fatty acids than the growth rate and the fermentable carbon consumption of the microorganism in the absence of the fatty acids. The addition of the fatty acids can increase glucose consumption, and can improve microorganism biomass production (cell growth/density) and growth rate, thereby reducing production time and increasing productivity of the fermentation process. | 12-22-2011 |
| 20110318798 | REMOVAL OF INHIBITORS OF MICROBIAL FERMENTATION FROM INHIBITOR-CONTAINING COMPOSITIONS - Methods are provided for conditioning an inhibitor-containing composition, such as a cellulosic biomass hydrolysate, to remove inhibitors of microbial growth and/or product production. The methods include precipitation of inhibitors by formation of complexes with metal salts, such as aluminum sulfate and ferric chloride. | 12-29-2011 |
| 20110318799 | YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD - There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical. | 12-29-2011 |
| 20120015416 | EXPRESSION OF HEXOSE KINASE IN RECOMBINANT HOST CELLS - The invention relates to a recombinant host cell having (a) a modification in an endogenous polynucleotide encoding a polypeptide having dual-role hexokinase activity; (b) a heterologous polynucleotide encoding a polypeptide having hexose kinase activity; and optionally (c) a modification in an endogenous polynucleotide encoding a polypeptide having pyruvate decarboxylase activity. Additionally, the invention relates to methods of making and using such recombinant host cells including, for example, methods of increasing glucose consumption, methods of improving redox balance, and/or methods of increasing the production of a product of a pyruvate-utilizing pathway. | 01-19-2012 |
| 20120015417 | METHODS OF INCREASING DIHYDROXY ACID DEHYDRATASE ACTIVITY TO IMPROVE PRODUCTION OF FUELS, CHEMICALS, AND AMINO ACIDS - The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 01-19-2012 |
| 20120028322 | METHODS OF INCREASING DIHYDROXY ACID DEHYDRATASE ACTIVITY TO IMPROVE PRODUCTION OF FUELS, CHEMICALS, AND AMINO ACIDS - The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 02-02-2012 |
| 20120028323 | YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD - The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 02-02-2012 |
| 20120028324 | ENGINEERED MICROORGANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS - The present invention is generlly provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention. | 02-02-2012 |
| 20120034666 | METHODS OF INCREASING DIHYDROXY ACID DEHYDRATASE ACTIVITY TO IMPROVE PRODUCTION OF FUELS, CHEMICALS, AND AMINO ACIDS - The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Nfs1 proteins or homologs thereof and/or one or more polynucleotides encoding one or more Isd11 proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 02-09-2012 |
| 20120040427 | Process for the production of ethanol and butanol - The present invention relates to a process for the production of ethanol and butanol from biomass, and in particular to a process for the production of ethanol and butanol using two separate fermentation steps, said process comprising: a) passing a biomass feedstock to a first fermentation step, b) in said first fermentation step subjecting the biomass feedstock to anaerobic fermentation at a pH below 6.0 and at a temperature in the range 20 to 700° C. and so as to convert the biomass to a product predominantly comprising acetic acid and butyric acid with at least a 2:1 ratio by weight of acetic acid to butyric acid, c) treating the product stream of step (b) to separate a solution comprising the acetic acid and butyric acid by: (i) separating a solution comprising the acetic acid and butyric acid from any residual solids and (ii) separating bacteria and/or pasteurising or sterilising the solution from the first fermentation step, and d) in a second fermentation step fermenting the solution comprising the acetic acid and butyric acid from step (c) to form ethanol and butanol. | 02-16-2012 |
| 20120045809 | Engineered Microorganisms for the Production of One or More Target Compounds - The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms comprise isobutanol producing metabolic pathway with one or more isobutanol pathway enzymes localized in the mitochondria. In various embodiments described herein, the recombinant microorganisms may be Crabtree-negative yeast microorganisms, microorganisms of the | 02-23-2012 |
| 20120058532 | ENGINEERED MICROORGANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS - The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention. | 03-08-2012 |
| 20120064590 | RECOVERY OF HIGHER ALCOHOLS FROM DILUTE AQUEOUS SOLUTIONS - This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs. | 03-15-2012 |
| 20120088281 | Thiolase with Improved Activity and Method of Producing Biobutanol Using the Same - The present invention relates to a modified thiolase protein with an improved activity, a polynucleotide encoding the modified protein, an expression vector including the polynucleotide, and a transformant, to a composition for producing a biobutanol including the thiolase with an improved activity or a cell expressing the thiolase, and to a method of producing the biobutanol. | 04-12-2012 |
| 20120107890 | RECOVERY OF HIGHER ALCOHOLS FROM DILUTE AQUEOUS SOLUTIONS - This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs. | 05-03-2012 |
| 20120107891 | RECOVERY OF HIGHER ALCOHOLS FROM DILUTE AQUEOUS SOLUTIONS - This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs. | 05-03-2012 |
| 20120115196 | CORYNEFORM BACTERIUM TRANSFORMANT AND PROCESS FOR PRODUCING ISOBUTANOL USING THE SAME | 05-10-2012 |
| 20120115197 | Ketol-Acid Reductoisomerase Using NADH - Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from | 05-10-2012 |
| 20120142065 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to produce ethanol and/or butanol, e.g., by fermentation. | 06-07-2012 |
| 20120149080 | YEAST PRODUCTION CULTURE FOR THE PRODUCTION OF BUTANOL - High cell density cultures of yeast were found to have higher tolerance for butanol in the medium. The high cell density yeast cultures had greater survival and higher glucose utilization than cultures with low cell densities. Production of butanol using yeast in high cell density cultures is thus beneficial for improving butanol production. | 06-14-2012 |
| 20120190089 | ENGINEERED MICROOGRANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS - The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention. | 07-26-2012 |
| 20120190090 | MICROBIAL CONVERSION OF PLANT BIOMASS TO ADVANCED BIOFUELS - The invention provides consolidated bioprocessing methods and host cells. The host cells are capable of directly converting biomass polymers or sunlight into alcohols or branched-chain hydrocarbons. In particular, the invention provides a method for producing alcohols or branched-chain hydrocarbons from a biomass polymer, including providing a genetically engineered host cell, culturing the host cell in a medium containing a biomass polymer as a carbon source such that recombinant nucleic acids in the cell are expressed, and extracting alcohols or branched-chain hydrocarbons from the culture. | 07-26-2012 |
| 20120196341 | FERMENTIVE PRODUCTION OF FOUR CARBON ALCOHOLS - Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein. | 08-02-2012 |
| 20120208250 | PROCESS FOR PRODUCING ALCOHOL AND OTHER BIOPRODUCTS FROM BIOMASS EXTRACTS IN A KRAFT PULP MILL - A method for the production of alcohol and other bioproducts from power boiler woody biomass extract containing hemicelluloses, with or without combining extract from wood prior to Kraft cooking. The process is integrated with the host Kraft pulp mill plant process to minimize the heat loss from extracting hemicelluloses and the energy used in the process. | 08-16-2012 |
| 20120220003 | METHOD FOR CONTINUOUSLY SEPARATING ORGANIC MATERIALS OF INTEREST FROM FERMENTATION - A method for continuously separating organic materials of interest from fermentation, in particular lactic or alcoholic fermentation, by flash evaporation is described. The method can make it possible to avoid inhibiting the fermentation reaction and to obtain high yields and productivity. | 08-30-2012 |
| 20120225464 | Specific Arabinose Transporter of the Plant Arabidopsis Thaliana for the Construction of Pentose-Fermenting Yeasts - The present invention relates to methods for conferring upon a cell the ability to take up L-arabinose, wherein said method comprises transforming the cell with a nucleic acid molecule comprising a nucleic acid sequence that codes for a plant pentose transporter, wherein the transformation enables the cell to take up L-arabinose. The present invention further relates to yeast cells that are transformed with a nucleic acid expression construct that codes for a plant pentose transporter, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to take up L-arabinose. Said cells are preferably utilized for the conversion/metabolization of biomaterial containing pentose(s), in particular arabinose, and particularly for the production of bio-based chemicals and biofuels. | 09-06-2012 |
| 20120231515 | BUTANOL DEHYDROGENASE ENZYME FROM THE BACTERIUM ACHROMOBACTER XYLOSOXIDANS - From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of | 09-13-2012 |
| 20120252084 | Enhanced Pyruvate to Acetolactate Conversion in Yeast - A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activity and reduced glycerol-3-phosphate dehydrogenase activity. Production of compounds having acetolactate as an upstream intermediate benefit from the increased conversion of pruvate to acetolactate in the described strains. | 10-04-2012 |
| 20120270288 | PROCESS TO PRODUCE ORGANIC COMPOUNDS FROM SYNTHESIS GASES - At least one isolated microorganism and a fermentation method to convert hydrogen gas, carbon dioxide gas, and/or carbon monoxide gas to a lower alkyl alcohol and/or carboxylic acid and to produce at least 2% by volume of the lower alkyl alcohol or carboxylic acid in an aqueous-based medium. | 10-25-2012 |
| 20120288910 | METHODS OF INCREASING DIHYDROXY ACID DEHYDRATASE ACTIVITY TO IMPROVE PRODUCTION OF FUELS, CHEMICALS, AND AMINO ACIDS - The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 11-15-2012 |
| 20120301936 | RECOMBINANT MUTANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE ALCOHOLS AND METHOD OF PRODUCING ALCOHOLS USING THE SAME - The present invention relates to recombinant mutant microorganisms having an increased ability to produce alcohol and a method of producing alcohol using the same, and more particularly to recombinant mutant microorganisms which have an increased ability to produce butanol, ethanol, isopropanol or mixed alcohols, which can be used as fuel, while producing little or no producing acetone as a byproduct, and to a method of producing butanol, ethanol, isopropanol or mixed alcohols using the same. The inventive recombinant mutant microorganisms having an increased ability to produce butanol or mixed alcohols and to remove acetone are those in which genes that encode enzymes involved in producing butanol from butyryl-CoA or butylaldehyde and in producing isopropanol from acetone were amplified or introduced in host microorganisms. The recombinant mutant microorganisms produce little or no byproducts such as acetone and can have an increased ability to produce alcohols, as a result of manipulating metabolic pathways. Thus, the recombinant mutant microorganisms are useful for industrial production of butanol or mixed alcohols comprising butanol and isopropanol. | 11-29-2012 |
| 20120322118 | INDIRECT PRODUCTION OF BUTANOL AND HEXANOL - A process for the conversion of carbohydrates from any of a number of sources into butanol and hexanol for fuel or chemical use is disclosed. The process includes conducting a homoacetogenic fermentation to produce an acetic acid intermediate which is chemically converted to ethanol. The ethanol and a remaining portion of the acetic acid intermediate are used as a substrate in an acidogenic fermentation to produce butyric and caproic acid intermediates which are then chemically converted to butanol and hexanol. | 12-20-2012 |
| 20130017588 | RECOMBINANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE BUTANOL AND METHOD OF PRODUCING BUTANOL USING THE SAMEAANM Lee; Sang YupAACI DaejeonAACO KRAAGP Lee; Sang Yup Daejeon KRAANM Jang; Yu-SinAACI DaejeonAACO KRAAGP Jang; Yu-Sin Daejeon KRAANM Lee; Jin YoungAACI IncheonAACO KRAAGP Lee; Jin Young Incheon KRAANM Lee; Joung MinAACI Gangwon-doAACO KRAAGP Lee; Joung Min Gangwon-do KRAANM Park; Jin HwanAACI DaejeonAACO KRAAGP Park; Jin Hwan Daejeon KR - The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels. | 01-17-2013 |
| 20130023020 | COOLING AND PROCESSING MATERIALS - Systems and methods for cooling and processing materials are disclosed. | 01-24-2013 |
| 20130059354 | ALKANOL - The present invention provides a process for the production of an alkanol (e.g. butanol) comprising converting a carbon source such as a biomass into a carboxylic acid or a derivative thereof using a microorganism, and electrochemically reducing said acid to said alkanol, as well as an apparatus for carrying out said process. | 03-07-2013 |
| 20130071901 | PROCESS FOR THE CONVERSION OF A XYLAN-CONTAINING FEED - A xylan-containing feed is contacted, in the first reactor essentially operated at plug flow, with an aqueous solution of an acid at a temperature in the range from equal to or more than 140° C. to equal to or less than 210° C. to produce an intermediate product. Then, the intermediate product is contacted, in the second reactor which comprises a continuously stirred tank reactor, with an aqueous solution of an acid at a temperature in the range from more than 130° C. to equal to or less than 200° C. to produce a final product. | 03-21-2013 |
| 20130084613 | PROCESSING BIOMASS - Carbon-containing materials, such as biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) or coal are processed to produce useful products, such as fuels. For example, systems are described that can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol. | 04-04-2013 |
| 20130084614 | METHODS OF REMOVING INHIBITORS FROM CELLULOSIC BIOMASS AND PRODUCING ALCOHOLS - A method of removing a fermentation inhibitor from an aqueous mixture of hydrolysis products from cellulosic biomass is disclosed. The method includes providing an aqueous mixture of hydrolysis products from cellulosic biomass, the hydrolysis products comprising at least one of pentose or hexose sugars and a fermentation inhibitor that inhibits a microorganism otherwise capable of fermenting pentose or hexose sugars; and at least partially extracting the fermentation inhibitor from the aqueous mixture with a first extractant by a first liquid-liquid extraction through a first porous membrane to provide a first extract and a raffmate, the first extractant having a water solubility of less than one percent by weight. In some embodiments, the aqueous mixture contains an insufficient amount of the microorganism to ferment the aqueous mixture. In some embodiments, the aqueous mixture is a fermentation broth. A membrane solvent extraction system for carrying out the method is also disclosed. | 04-04-2013 |
| 20130089905 | METHOD TO REDUCE GHG EMISSIONS OF FUEL PRODUCTION - The present invention provides a method for reducing life cycle GHG emissions associated with production of a liquid fuel or fuel intermediate. The method comprises: fermenting sugar to produce biogenic carbon dioxide and the liquid fuel or fuel intermediate; collecting an amount of biogenic carbon dioxide generated from the fermentation; and supplying the biogenic carbon dioxide for use in one or more enhanced oil or gas recovery sites for displacement of geologic carbon dioxide. Further provided is a method comprising receiving an amount of carbon dioxide from an apparatus for delivering carbon dioxide to one or more enhanced oil or gas recovery sites so as to displace the use of geologic carbon dioxide at the site. The carbon dioxide received has the GHG emission attributes of the biogenic carbon dioxide introduced to the apparatus. | 04-11-2013 |
| 20130122561 | RECOVERY OF HIGHER ALCOHOLS FROM DILUTE AQUEOUS SOLUTIONS - This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allow recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs. | 05-16-2013 |
| 20130130342 | Yeast Strain for Production of Four Carbon Alcohols - Yeast cells with a reduced general control response to amino acid starvation were found to have increased tolerance to butanol in the growth medium. The reduced response was engineered by genetic modification of a gene involved in the response, a GCN gene, to eliminate activity of the encoded protein. Yeast strains with an engineered butanol biosynthetic pathway and a genetic modification in a gene involved in the general control response to amino acid starvation, which have increased butanol tolerance, are useful for production of butanol. | 05-23-2013 |
| 20130149757 | Method for Producing Butanol and Isopropanol | 06-13-2013 |
| 20130149758 | Conversion System For Biomass - The efficient production of ethanol from low-cost biomass (e.g., corn, sugar beets, sugar cane, switchgrass and/or paper) has become increasingly important in making ethanol competitive with gasoline and decreasing the United States' dependence on foreign oil. For example, to reduce the cost of transporting biomass to ethanol production facilities, mobile systems for producing ethanol from biomass are provided. Also provided are small-scale ethanol production facilities. For example, instead of transporting biomass to the production facility, the facility is transported to the biomass or is located nearby the source of the biomass. The ethanol production facilities or components thereof may be transported via land, water, or air. Production of other products, such as hydrocarbons, natural gas, hydrogen gas, plastics, polymers, and proteins, can also be made by the methods and facilities. Any product described herein can be made in finished form or un-finished form and moved, e.g., to a fixed facility, e.g., fixed production facility. | 06-13-2013 |
| 20130171706 | Fermentive Production of Four Carbon Alcohols - Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway. | 07-04-2013 |
| 20130177957 | SULFIDE GENERATION PROCESS AND SYSTEM FOR SYNGAS FERMENTATION - A process that generates sulfide in production of liquid products from syngas and a system for syngas fermentation including a sulfide generation process to convert feed gas to liquid product, the process including: partially oxidizing high-temperature gas, input gas, and molten sulfur to generate sulfide gas, the high-temperature gas containing oxygen, the input gas containing carbonaceous fuel and the sulfide gas containing hydrogen sulfide, CO, CO | 07-11-2013 |
| 20130183730 | RECOVERY OF HIGHER ALCOHOLS FROM DILUTE AQUEOUS SOLUTIONS - This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs. | 07-18-2013 |
| 20130183731 | FERMENTIVE PRODUCTION OF FOUR CARBON ALCOHOLS - Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway. | 07-18-2013 |
| 20130196398 | METHODS OF HYDROLYZING PRETREATED DENSIFIED BIOMASS PARTICULATES AND SYSTEMS RELATED THERETO - A method is provided in which pretreated and densified cellulosic biomass particulates can hydrolyzed at a high solids loading rate as compared with the solids loading rate of loose hydrolysable cellulosic biomass fibers. The resulting high concentration sugar-containing stream can be easily converted to biofuels or an entire suite of other useful bioproducts. | 08-01-2013 |
| 20130236940 | METHOD FOR THE OXIDATION OF ORGANIC COMPOUNDS - A method is described for the oxidation of organic compounds in which the organic compound that is to be oxidized is brought to reaction in an aqueous solution in the presence of cytochrome P 450, and wherein perfluorinated organic compound is added to the aqueous reaction mixture as activator. | 09-12-2013 |
| 20130244299 | Continuous Single Vessel Butanol Synthesis by Fermentation - The present invention describes a method for producing butanol by fermentation of carbohydrates using mixed populations of acidogenic-phase cells and solventogenic-phase cells of | 09-19-2013 |
| 20130252296 | ACETATE SUPPLEMENTION OF MEDIUM FOR BUTANOLOGENS - The invention relates to the fields of industrial microbiology and alcohol production. More specifically, the invention relates to improved production of butanol isomers by recombinant microorganisms containing an engineered butanol pathway and disrupted activity of the genes in pathways for the production of by-products during the fermentation when the microorganisms are grown in a fermentation medium containing acetate. In embodiments, recombinant microorganisms have an increased growth rate in a fermentation medium containing acetate as a C2 supplement. | 09-26-2013 |
| 20130252297 | Method for Controlling Butanol Concentration in Fermentation Broth - The present invention relates to a two stage process to control the butanol concentration from fermentation broth. | 09-26-2013 |
| 20130252298 | Yeast Organism Producing Isobutanol at a High Yield - The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 09-26-2013 |
| 20130295628 | PROCESSES FOR PRODUCING ENERGY-DENSE BIOMASS AND SUGARS OR SUGAR DERIVATIVES, BY INTEGRATED HYDROLYSIS AND TORREFACTION - This invention provides processes to convert biomass into energy-dense biomass for combustion, alone or in combination with another solid fuel. In some variations, biomass is extracted to produce an extract liquor containing hemicellulosic oligomers and cellulose-rich solids; hemicellulosic oligomers are removed; and the cellulose-rich solids are torrefied to produce energy-dense biomass. In some embodiments, hydrotorrefaction is employed to produce hydrophobic, energy-dense biomass in an energy-efficient process that avoids intermediate drying between extraction/hydrolysis and torrefaction. The energy-dense biomass may be pelletized or directly combusted or gasified. The hemicellulosic oligomers may be hydrolyzed to fermentable sugars and then fermented to ethanol or other products, or further reacted to produce furfural or other products. | 11-07-2013 |
| 20130295629 | CONTINUOUS OR SEMI-CONTINUOUS PROCESS FOR TREATING BIOMASS TO PRODUCE MATERIALS USEFUL FOR BIOFUELS - Fermentable sugar useful for the production of biofuels is produced from biomass in a continuous or semi-continuous manner by providing pumpable biomass. | 11-07-2013 |
| 20130302868 | Isobutanol Production Using Yeasts With Modified Transporter Expression - The present invention relates to recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise one or more modifications resulting in the reduction in the expression and/or activity of an endogenous transporter protein. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 11-14-2013 |
| 20130302869 | OPTOFLUIDIC PHOTOBIOREACTOR APPARATUS, METHOD, AND APPLICATIONS - An optofluidic photobioreactor including an optical waveguide having an input, characterized by an evanescent optical field confined along an outer surface of the optical waveguide produced by radiation propagating in the optical waveguide, means for inputting light to the input of the optical waveguide, and a selected photosynthetic microorganism disposed substantially within the evanescent field. A method for optically exciting a photosynthetic microorganism for generating a biofuel, a biofuel precursor, or a biomass from the optically-excited photosynthetic microorganism involves irradiating the photosynthetic microorganism attached to the surface of the waveguide with an evanescent optical field from optical radiation propagating in the optical waveguide, and driving photosynthesis in the microorganism by the evanescent optical field. | 11-14-2013 |
| 20130309738 | Processes and Systems for Alcohol Production and Recovery - The present invention relates to processes for recovering butanol produced in a fermentative process using, for example, an ethanol production plant which has been reversibly-retrofitted for butanol production, that is, the ethanol production plant may be converted for butanol production, but can also revert to an ethanol production. The present invention also relates to processes for recovering butanol produced in a fermentative process in a butanol production plant that may be converted to ethanol production plant. | 11-21-2013 |
| 20130323809 | METABOLIC ENGINEERING OF CLOSTRIDIUM TYROBUTYRICUM FOR BUTANOL PRODUCTION - This invention relates to compositions, systems, and methods for producing biofuels, such as butanol, and related compounds. More specifically, provided are methods of making recombinant microorganisms having non-naturally occurring metabolic pathways for the production of biofuels, and methods of producing biofuels using such organisms. Also provided are metabolically engineered microorganisms capable of producing butanol from a substrate. | 12-05-2013 |
| 20130344553 | Designer Calvin-Cycle-Channeled and Hydrogenotrophic Production of Butanol and Related Higher Alcohols - Designer Calvin-cycle-channeled and hydrogenotrophic biofuel-production pathways, the associated designer genes and designer transgenic organisms for autotrophic production of butanol and related higher alcohols from carbon dioxide, hydrogen, and/or water are provided. The butanol and related higher alcohols include 1-butanol, 2-methyl-1-butanol, isobutanol, 3-methyl-1-butanol, 1-hexanol, 1-octanol, 1-pentanol, 1-heptanol, 3-methyl-1-pentanol, 4-methyl-1-hexanol, 5-methyl-1-heptanol, 4-methyl-1-pentanol, 5-methyl-1-hexanol, and 6-methyl-1-heptanol. The designer autotrophic organisms such as designer transgenic oxyphotobacteria and algae comprise designer Calvin-cycle-channeled and hydrogenotrophic pathway gene(s) and biosafety-guarding technology for enhanced autotrophic production of butanol and related higher alcohols from carbon dioxide and water. | 12-26-2013 |
| 20140004584 | Methods And Systems For Producing Products Using Engineered Iron Oxidizing Bacteria | 01-02-2014 |
| 20140017748 | MODIFIED ALCOHOL DEHYDROGENASES FOR THE PRODUCTION OF FUELS AND CHEMICALS - The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 01-16-2014 |
| 20140024094 | INCREASED PRODUCTION OF ISOBUTANOL IN YEAST WITH REDUCED MITOCHONDRIAL AMINO ACID BIOSYNTHESIS - Yeast cells with reduced activity of certain enzymes involved in branched chain amino acid biosynthesis in yeast mitochondria are described. Target enzymes include threonine deaminase, isopropylmalate synthase, and optionally branched chain amino acid transaminase. | 01-23-2014 |
| 20140030782 | HOST CELLS AND METHODS FOR PRODUCTION OF ISOBUTANOL - Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity. | 01-30-2014 |
| 20140030783 | HOST CELLS AND METHODS FOR PRODUCTION OF ISOBUTANOL - Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity. | 01-30-2014 |
| 20140038251 | PROCESSING BIOMASS - Fructose, e.g., fructose derived from a cellulosic or lignocellulosic material, is use, e.g., fermented to produce a product, e.g., a solvent. | 02-06-2014 |
| 20140038252 | PROCESS FOR THE PRODUCTION OF ETHANOL AND BUTANOL - The present invention relates to a process for the production of ethanol and butanol from biomass, and in particular to a process for the production of ethanol and butanol using two separate fermentation step subjecting the biomass feedstock to anaerobic fermentation at a pH below 6.0 and at a temperature in the range 20 to 700° C. and so as to convert the biomass to a product predominantly comprising acetic acid and butyric acid with at least a 2:1 ratio by weight of acetic acid to butyric acid, c) treating the product of stream of step (b) to separate a solution comprising the acetic acid and butyric acid by: (i) separating a solution comprising the acetic acid and butyric acid from any residual solids and (ii) separating bacteria and/or pasteurizing or sterilizing the solution from the first fermentation step, and d) in a second fermentation step fermenting the solution comprising the acetic acid and butyric acid from the step (c) to form ethanol and butanol. | 02-06-2014 |
| 20140045234 | Applications of the rotating photobioreactor - A method to recover and harvest nutrients from a liquid stream by incorporating them into microorganisms grown in a rotating photobioreactor. The method further includes optionally integrating the rotating photobioreactor with a composting or biogenic drying process. | 02-13-2014 |
| 20140057327 | SINGLE VESSEL PRODUCTION OF BUTANOL FROM BIOMASS USING ENGINEERED THERMOPHILIC MICROORGANISMS - The present invention relates to the production of biofuels by utilizing efficient biomass digestion and fermentation by a new thermophilic microorganism generated after fusion of two different bacteria | 02-27-2014 |
| 20140065682 | PROCESSES AND APPARATUS FOR PRODUCING FERMENTABLE SUGARS, CELLULOSE SOLIDS, AND LIGNIN FROM LIGNOCELLULOSIC BIOMASS - Variations of this invention reduce or avoid lignin precipitation during acidic hydrolysis of biomass hydrolysates (such as hemicellulose-containing liquid extracts). Net acid usage and byproduct salt formation are significantly reduced. In some embodiments, hemicellulosic oligomers are hydrolyzed, in the presence of sulfur dioxide, to produce fermentable hemicellulosic sugars; the process comprising recovering and recycling at least a portion of the sulfur dioxide, wherein at least a portion of the sulfur dioxide reacts with the lignin to produce hydrophilic sulfonated lignin that has less tendency to precipitate or stick. In other embodiments, hemicellulosic oligomers are hydrolyzed, in the presence of a catalyst selected from the group consisting of sulfuric acid, sulfurous acid, sulfur dioxide, and combinations thereof, and an additive selected from metal sulfites, metal bisulfites, and combinations thereof, to produce fermentable hemicellulosic sugars, wherein at least a portion of the additive reacts with the lignin to produce sulfonated lignin. | 03-06-2014 |
| 20140065683 | METHOD FOR PRODUCING ETHANOL AND SOLVENTS FROM LIGNOCELLULOSIC BIOMASS INCLUDING THE RECIRCULATION OF A BUTYL WINE OBTAINED BY FERMENTING PENTOSES - A process for the production of alcohols and/or solvents starting from cellulosic or lignocellulosic biomass comprising at least:
| 03-06-2014 |
| 20140080188 | YEAST MICROORGANISMS WITH REDUCED 2,3-BUTANEDIOL ACCUMULATION FOR IMPROVED PRODUCTION OF FUELS, CHEMICALS, AND AMINO ACIDS - The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of an acetolactate-derived by-product such as 2,3-butanediol. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 03-20-2014 |
| 20140080189 | Method for Producing Butanol using Extractive Fermentation with Electrolyte Addition - A method for producing butanol through microbial fermentation, in which the butanol product is removed during the fermentation by extraction into a water-immiscible organic extractant in the presence of at least one electrolyte at a concentration at least sufficient to increase the butanol partition coefficient relative to that in the presence of the salt concentration of the basal fermentation medium, is provided. The electrolyte may comprise a salt which dissociates in the fermentation medium, or in the aqueous phase of a biphasic fermentation medium, to form free ions. Also provided is a method and composition for recovering butanol from a fermentation medium. | 03-20-2014 |
| 20140087436 | AUTOTROPHIC HYDROGEN BACTERIA AND USES THEREOF - In an aspect, the invention relates to compositions and methods production of n-butanol by aerobic hydrogen bacteria. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention. | 03-27-2014 |
| 20140087437 | PROCESS FOR THE CRYSTALLISATION OF A WATER-SOLUBLE COMPOUND - A process for the crystallisation of a water-soluble compound from a solution of water and a solvent is provided. A process for the manufacture of crystalline sucrose from sugar palm juice or sucrose-containing biomass comprising such crystallisation process is also disclosed. | 03-27-2014 |
| 20140093930 | Polypeptides with Ketol-Acid Reductoisomerase Activity - Polypeptides having ketol-acid reductoisomerase activity are provided. Also disclosed are recombinant host cells comprising isobutanol biosynthetic pathways employing such polypeptides. Methods for producing isobutanol employing host cells comprising the polypeptides having ketol-acid reductoisomerase activity are also disclosed. | 04-03-2014 |
| 20140093931 | PRODUCTION OF FERMENTATION PRODUCTS - The invention relates to processes for the production of fermentation products such as alcohols including ethanol and butanol, and the development of microorganisms capable of producing fermentation products via an engineered pathway in the microorganisms. | 04-03-2014 |
| 20140099688 | PROCESS TO REMOVE PRODUCT ALCOHOL FROM A FERMENTATION BY VAPORIZATION UNDER VACUUM - A fermentation liquid feed including water and a product alcohol and optionally CO | 04-10-2014 |
| 20140106424 | Reducing Carbon Dioxide Production and Increasing Ethanol Yield During Microbial Ethanol Fermentation - The present invention provides compositions and methods for producing ethanol wherein the amount of CO | 04-17-2014 |
| 20140106425 | BIOPROCESSES FOR MAKING BUTANOL - Butanol is produced by the bioconversion of substrate using a biocatalyst comprising an open, porous hydrophilic polymeric structure having microorganisms for the bioconversion irreversibly retained therein wherein the microorganisms have undergone phenotypic alterations which includes enhanced tolerance to butanol. | 04-17-2014 |
| 20140141479 | Expression of Hexose Kinase in Recombinant Host Cells - The invention relates to a recombinant host cell having (a) a modification in an endogenous polynucleotide encoding a polypeptide having dual-role hexokinase activity; (b) a heterologous polynucleotide encoding a polypeptide having hexose kinase activity; and optionally (c) a modification in an endogenous polynucleotide encoding a polypeptide having pyruvate decarboxylase activity. Additionally, the invention relates to methods of making and using such recombinant host cells including, for example, methods of increasing glucose consumption, methods of improving redox balance, and/or methods of increasing the production of a product of a pyruvate-utilizing pathway. | 05-22-2014 |
| 20140147901 | IMMOBILIZED PRODUCT TOLERANT MICROORGANISMS - Methods for adapting or selecting microorganisms with increased product tolerance are provided. Additionally, a bioreactor capable of operation in either packed bed or fluidized bed is disclosed along with methods to use the bioreactor for culturing microorganisms adapted or selected increased product tolerance. | 05-29-2014 |
| 20140162333 | Enhanced Pyruvate to Acetolactate Conversion in Yeast - A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activity and reduced glycerol-3-phosphate dehydrogenase activity. Production of compounds having acetolactate as an upstream intermediate benefit from the increased conversion of pruvate to acetolactate in the described strains. | 06-12-2014 |
| 20140170722 | METHOD FOR PRODUCING ISOBUTANOL AND RECOMBINANT MICROORGANISM CAPABLE OF PRODUCING ISOBUTANOL - This invention is intended to produce isobutanol with excellent productivity via a fermentation process. A reaction in which NADP-dependent isocitrate dehydrogenase generates NADPH from NADP is used as a source of NADPH for the reaction of converting 2-acetolactate into 2,3-dihydroxy-isovalerate in the isobutanol biosynthesis pathway. | 06-19-2014 |
| 20140178955 | BUTANOL FERMENTATION USING ACID PRETREATED BIOMASS - The present invention provides the use of acid pretreated biomass such as | 06-26-2014 |
| 20140186911 | RECOMBINANT HOST CELLS AND METHODS FOR PRODUCING BUTANOL - Provided herein are recombinant yeast cells comprising a deletion or disruption in an endogenous gene encoding Amn1 and a heterologous gene encoding Amn1. Also provided are recombinant yeast cells comprising a heterologous gene encoding Amn1 and an engineered butanol biosynthetic pathway. Further provided are methods of producing isobutanol comprising providing the recombinant yeast cells described herein and culturing the recombinant yeast cells under conditions wherein isobutanol is produced. | 07-03-2014 |
| 20140206052 | Method for production of n-butanol from syngas using syntrophic co-cultures of anaerobic microorganisms - This invention provides methods and systems for the production of butanol. Specifically, the methods and systems of the present invention use syntrophic co-cultures for the production of butanol from syngas. | 07-24-2014 |
| 20140212941 | Designer Organisms for Photobiological Butanol Production from Carbon Dioxide and Water - The present invention provides a biosafety-guarded photobiological butanol production technology based on designer transgenic plants, designer algae, designer blue-green algae (cyanobacteria and oxychlorobacteria), or designer plant cells. The designer photosynthetic organisms are created such that the endogenous photobiological regulation mechanism is tamed, and the reducing power (NADPH) and energy (ATP) acquired from the photosynthetic process are used for synthesis of butanol (CH | 07-31-2014 |
| 20140220649 | METHOD FOR PRODUCING C4 OXYGENTATES BY FERMENTATION USING HIGH OXIDATION STATE SULFUR - The invention relates to improvements in the production of butanol and butyrate by microbial fermentation, particularly to production of alcohols by microbial fermentation of a substrate comprising CO and the addition of an inorganic sulfur additive. It more particularly relates to the provision of an inorganic organic sulfur source to a fermentation system such that one or more micro-organisms convert a substrate comprising CO to butanol. In one aspect the invention uses a sulfur additive comprising inorganic sulfur compounds having a +2 to a +4 sulfur oxidation state that produces sulfur oxoanions and hydrosulfur oxoanions in an aqueous fermentation medium. | 08-07-2014 |
| 20140234929 | PROCESSES AND SYSTEMS FOR ALCOHOL PRODUCTION AND RECOVERY - The present invention relates to processes for recovering butanol produced in a fermentative process using, for example, an ethanol production plant which has been reversibly-retrofitted for butanol production, that is, the ethanol production plant may be converted for butanol production, but can also revert to an ethanol production. The present invention also relates to processes for recovering butanol produced in a fermentative process in a butanol production plant that may be converted to ethanol production plant. | 08-21-2014 |
| 20140273125 | PROCESSES FOR THE ANAEROBIC BIOCONVERSION OF SYNGAS TO OXYGENATED ORGANIC COMPOUND WITH IN SITU PROTECTION FROM HYDROGEN CYANIDE - Processes are disclosed for the anaerobic bioconversion of syngas to oxygenated organic compound that use an in situ method for protecting the microorganisms from hydrogen cyanide contained in the syngas that passes to the fermentation broth. The fermentation broth is maintained at a pH of between about 4 and 6, and dissolved metal cation of one or more of iron, cobalt, nickel and zinc is provided to the fermentation broth in an amount sufficient to form, under the conditions of the fermentation broth, a substantially insoluble metal complex with the metal cation and cyanide anion. The rate of formation of the insoluble complex is sufficiently high that that the amount of cyanide that is taken up by microorganisms does not result in an undue adverse effect on the population of microorganisms. | 09-18-2014 |
| 20140273126 | METHOD FOR INCREASING PRODUCTION OF PLANT BIOMASS - It is an object of the present invention to increase the biomass quantity of plants by causing an exogenous gene to be carried in plant cells. An exogenous gene that promotes double-stranded DNA breakage is introduced into the cells of a plant, and the plant is grown. | 09-18-2014 |
| 20140273127 | PROCESSES AND SYSTEMS FOR THE PRODUCTION OF ALCOHOLS - The present invention relates to processes and systems for the production of fermentative products such as ethanol and butanol. The present invention also provides methods for separating feed stream components for improved biomass processing productivity. | 09-18-2014 |
| 20140273128 | Biological Conversion of Multi-Carbon Compounds from Methane - Multi-carbon compounds such as ethanol, n-butanol, sec-butanol, isobutanol, tert-butanol, fatty (or aliphatic long chain) alcohols, fatty acid methyl esters, 2,3-butanediol and the like, are important industrial commodity chemicals with a variety of applications. The present invention provides metabolically engineered host microorganisms which metabolize methane (CH | 09-18-2014 |
| 20140273129 | GLYCEROL 3- PHOSPHATE DEHYDROGENASE FOR BUTANOL PRODUCTION - Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased K | 09-18-2014 |
| 20140273130 | COMPETITIVE GROWTH AND/OR PRODUCTION ADVANTAGE FOR BUTANOLOGEN MICROORGANISM - Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products. Host cells provided herein comprise a pyruvate-utilizing pathway and a competitive growth advantage over other microorganisms in solution. | 09-18-2014 |
| 20140295512 | Ketol-Acid Reductoisomerases With Improved Performance Properties - The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or modified NADH-dependent variant thereof, wherein said KARI is at least about 60% identical to SEQ ID NO: 2. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol. | 10-02-2014 |
| 20140295513 | High-Performance Ketol-Acid Reductoisomerases - The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or a modified NADH-dependent variant thereof, wherein said KARI is at least about 80% identical to SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 28, SEQ ID NO: 40, or SEQ ID NO: 58. The present invention also relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or a modified NADH-dependent variant thereof, wherein said KARI is at least about 99% identical to SEQ ID NO: 64. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol. | 10-02-2014 |
| 20140302576 | Processes of Producing Fermentation Products - The invention relates to a process of fermenting plant material in a fermentation medium into a fermentation product using a fermenting organism, wherein one or more carbonic anhydrases are present in the fermentation medium. | 10-09-2014 |
| 20140308721 | Cytosolic Isobutanol Pathway Localization for the Production of Isobutanol - The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and/or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 10-16-2014 |
| 20140322780 | Methylbutanol as an Advanced Biofuel - This invention describes genes, metabolic pathways, microbial strains and methods to produce methylbutanol and other compounds of interest from renewable feedstocks. | 10-30-2014 |
| 20140335582 | FERMENTIVE PRODUCTION OF FOUR CARBON ALCOHOLS - Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway. | 11-13-2014 |
| 20140335583 | Methods and Systems for Producing Products Using Engineered Iron Oxidizing Bacteria - Methods and systems for producing a biofuel using genetically modified iron-oxidizing bacteria (IOB) are disclosed. In some embodiments, the methods include the following: providing an IOB that have been genetically modified to enable them to generate a biofuel or chemical; feeding a first source of ferrous iron to the IOB; feeding water, carbon dioxide, and oxygen to the IOB; producing at least the biofuel or chemical, ferric iron, and an IOB biomass; and preventing ferric precipitates from forming. In some embodiments, the methods and systems include the following: a bioreactor including IOB that have been genetically modified to enable them to generate a biofuel; a first source of ferrous iron; sources of water, carbon dioxide, and oxygen; a source of anti-ferric precipitating agent in fluid communication with the bioreactor; and a electrochemical reactor that is configured to electrochemically reduce ferric iron to a second source of ferrous iron. | 11-13-2014 |
| 20140342420 | PRODUCTION OF BIO-BUTANOL AND RELATED PRODUCTS - The present invention provides a method for the production of an alkanol from biomass. The method for producing an alkanol, including n-butanol, comprises fermenting a carbohydrate to produce a diacid and converting the diacid into an alkanol by solution chemistry or solid phase chemistry. The carbohydrate may be derived or extracted from a lignocellulose, an algae, a grain, or a starch containing root. | 11-20-2014 |
| 20140349357 | METHODS OF PRODUCING BUTANOL FROM NON-CELLULOSIC BIOMASS - Methods of recovering butanol, and preferably increasing the rate and/or yield of its production, from the fermentation of material derived from the digestion of non-cellulosic biomass. | 11-27-2014 |
| 20140356920 | RECOVERY OF HIGHER ALCOHOLS FROM DILUTE AQUEOUS SOLUTIONS - This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allow recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs. | 12-04-2014 |
| 20140363865 | SUPPLEMENTATION OF FATTY ACIDS FOR IMPROVING ALCOHOL PRODUCTIVITY - Fatty acids derived from biomass at a step in a fermentation process can be added to a fermentation medium comprising a recombinant microorganism that produces a product alcohol. At least one of growth rate and fermentable carbon consumption of the microorganism is greater in the presence of the fatty acids than the growth rate and the fermentable carbon consumption of the microorganism in the absence of the fatty acids. The addition of the fatty acids can increase glucose consumption, and can improve microorganism biomass production (cell growth/density) and growth rate, thereby reducing production time and increasing productivity of the fermentation process. | 12-11-2014 |
| 20140377824 | ALCOHOL DEHYDROGENASES (ADH) USEFUL FOR FERMENTIVE PRODUCTION OF LOWER ALKYL ALCOHOLS - The invention relates to suitable candidate ADH enzymes for production of lower alkyl alcohols including isobutanol. The invention also relates to recombinant host cells that comprise such ADH enzymes and methods for producing lower alkyl alcohols in the same. | 12-25-2014 |
| 20140377825 | PROCESS FOR THE BIOLOGICAL PRODUCTION OF n-BUTANOL WITH HIGH YIELD - The present invention provides a method for the biological production of n-butanol at high yield from a fermentable carbon source. In one aspect of the present invention, a process for the conversion of glucose to n-butanol is achieved by the use of a recombinant organism comprising a host | 12-25-2014 |
| 20150024448 | Integrated Processes for the Conversion of Coal to Chemicals - Integrated processes are disclosed for the conversion of coal containing mercury compounds, hydrocarbon-containing compounds, sulfur-containing compounds and nitrogen-containing compounds to chemicals in which coal is heated to remove mercury components, pyrolyzed to remove sulfur-containing and nitrogen-containing compounds and then gasified to provide syngas, and syngas is bioconverted to liquid product. | 01-22-2015 |
| 20150031102 | METHODS AND COMPOSITIONS FOR ENHANCED PRODUCTION OF BUTANOL BY CLOSTRIDIA - The invention relates generally to methods and compositions for maintaining and manipulating microbial cultures of Gram-positive bacteria. Also provided are methods for identifying quorum sensing regulatory proteins and auto-inducing peptides in Gram-positive bacteria. Also provided are methods and compositions believed to affect quorum sensing pathways of the genus | 01-29-2015 |
| 20150037855 | FERMENTATIVE PRODUCTION OF ALCOHOLS - The invention relates to the fields of industrial microbiology and alcohol production. The invention also relates to the development of a microorganism capable of producing fermentation products via an engineered pathway, and uses of the microorganism. The invention also relates to the methods to improve cell viability and productivity and the use of recycling and acid washing to increase the yield of fermentation products. | 02-05-2015 |
| 20150044747 | METHODS FOR INCREASING PRODUCTION OF 3-METHYL-2-BUTENOL USING FUSION PROTEINS - The invention relates, in part, to nucleic acid constructs, genetically modified host cells and methods employing such constructs and host cells to increase the production of 3-methyl-2-butenol from IPP. Thus, in some aspects, the invention provides a genetically modified host cell transformed with a nucleic acid construct encoding a fusion protein comprising a phosphatase capable of catalyzing the dephosphorylation of dimethylallyl diphosphate (DMAPP) linked to an IPP isomerase capable of converting IPP to DMAPP, wherein the nucleic acid construct is operably linked to a promoter. In some embodiments, the genetically modified host cell | 02-12-2015 |
| 20150050709 | PROCESSING BIOMASS - Some products such as solvents, e.g., butanol, are toxic to solvent-producing microorganisms, yet fructose metabolism can facilitate the increase of protective substrates such as triglycerides, which can protect the microorganism from the toxic effect of the product. Methods are provided for producing a product, e.g., a solvent from fructose derived from a cellulosic or lignocellulosic material. Using the methods herein, a fructose solution can be fermented to a solvent more rapidly, and with better yields, than a glucose solution. | 02-19-2015 |
| 20150064761 | PROCESS OF PRODUCING BIOENERGY WITH LOW CARBON DIOXIDE EMISSIONS AND ZERO-WASTE OF BIOMASS - Disclosed herein is a bioenergy production system with reduced carbon dioxide emissions and process wastes; including a process for producing a bioalcohol and a biogas by subjecting a biomass, such as: herbaceous and woody plants, fruit pulp, freshwater and sea algae, grains, aerobic and anaerobic sludge, saccharides, polyols and carbohydrates, to a combined process of a biosaccharification/alcohol fermentation, including a biomass pretreatment process; and a process for producing a methane biogas with a reduced level of carbon dioxide and hydrogen sulfide, via an algae cultivation process with a view to purifying the carbon dioxide and hydrogen sulfide contained in the biogas; wherein, when the algae to be cultivated is microalgae, biodiesel is produced by subjecting the harvested microalgae to a biodiesel manufacturing process while recycling the glycerol and the saccharide-containing waste produced as byproducts to the biosaccharification/alcohol fermentation process, and when the algae to be cultivated is macroalgae the harvested macroalgae is recycled to the biosaccharification/alcohol fermentation process. The method of the present invention is effective in reducing carbon dioxide emissions, a representative green house gas contributing to the global warming, and also in optimizing a zero-waste bioenergy production system. | 03-05-2015 |
| 20150079651 | Applications of the rotating photobioreactor - A method to recover and harvest nutrients and volatile gases such as alcohols from a liquid stream using a fixed film bioreactor. The method includes a means of concentrating product gas stripped from a bioreactor. | 03-19-2015 |
| 20150111269 | KETOL-ACID REDUCTOISOMERASE USING NADH - Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from | 04-23-2015 |
| 20150125920 | ENHANCED PYRUVATE TO ACETOLACTATE CONVERSION IN YEAST - A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activity and reduced glycerol-3-phosphate dehydrogenase activity. Production of compounds having acetolactate as an upstream intermediate benefit from the increased conversion of pruvate to acetolactate in the described strains. | 05-07-2015 |
| 20150299740 | RECOMBINANT MICROORGANISM HAVING ENHANCED BUTANOL PRODUCING ABILITY AND METHOD FOR PRODUCING BUTANOL USING THE SAME - The present invention relates to a microorganism having an acetyl CoA biosynthesis pathway and a butyryl CoA biosynthesis pathway; the microorganism being a recombinant microorganism having an increased ability to produce butanol, wherein a pathway for converting acetyl CoA into acetate is suppressed, and a pathway for converting acetate into acetyl CoA and a pathway for converting butyryl CoA into butanol are promoted. Also, the present invention concerns a method for producing butanol by using the recombinant microorganism. | 10-22-2015 |
| 20150307904 | Methods for the Economical Production of Biofuel from Biomass - Methods for producing a biofuel are provided. Also provided are biocatalysts that convert a feedstock to a biofuel. | 10-29-2015 |
| 20150353444 | PROCESS TO MAKE OLEFINS FROM ISOBUTANOL - A process for the conversion of an alcohol mixture may include introducing into a reactor a stream including the alcohol mixture mixed with a stream including olefins having 4 carbon atoms or more. The process may include contacting the stream with a single catalyst at a temperature above 500° C. in the reactor at conditions effective to dehydrate isobutanol, forming C | 12-10-2015 |
| 20150353961 | Designer Photoautotrophic and Hydrogenotrophic Production of Alcohols and Biodiesel - Designer Calvin-cycle-channeled and hydrogenotrophic biofuel-production pathways, the associated designer genes and designer transgenic organisms for autotrophic production of alcohols and biodiesel from carbon dioxide, hydrogen, and/or water are disclosed. The alcohols include methanol, ethanol, propanol, 1-butanol, 2-methyl-1-butanol, isobutanol, 3-methyl-1-butanol, 1-hexanol, 1-octanol, 1-pentanol, 1-heptanol, 3-methyl-1-pentanol, 4-methyl-1-hexanol, 5-methyl-1-heptanol, 4-methyl-1-pentanol, 5-methyl-1-hexanol, and 6-methyl-1-heptanol. The designer autotrophic organisms such as designer transgenic oxyphotobacteria and algae comprise designer Calvin-cycle-channeled and hydrogenotrophic pathway gene(s) and biosafety-guarding technology for enhanced autotrophic production of alcohols and biodiesel from carbon dioxide and water; wherein the designer transgenic cells in their mass liquid culture can inducibly self-flocculate for enhanced harvesting of their biomass upon the expression of the designer cell surface-linked positively charged polypeptides. | 12-10-2015 |
| 20150376655 | RECOMBINANT MICROORGANISM WITH IMPROVED BUTANOL PRODUCTION ABILITY AND METHOD FOR PRODUCING BUTANOL BY USING THE SAME - The present invention relates to a recombinant microorganism with improved butanol production ability which has an acetyl-CoA synthesis pathway and a butyryl-CoA synthesis pathway, wherein a pathway converting acetyl-CoA to acetate is inhibited and a pathway converting acetyl-CoA to butyryl-CoA is promoted. In addition, the present invention relates to a method for producing butanol using the recombinant microorganism. | 12-31-2015 |
| 20160010118 | METHODS FOR SUSTAINING THE VIABILITY OF ACETOGENS DURING A DECREASE OR CESSATION OF SYNGAS FLOW | 01-14-2016 |
| 20160010119 | PROCESSES FOR THE ACIDIC, ANAEROBIC CONVERSION OF HYDROGEN AND CARBON OXIDES TO OXYGENATED ORGANIC COMPOUND | 01-14-2016 |
| 20160010120 | METHOD OF PREPARING BUTANOL FROM A BUTYRIC ACID-CONTAINING AQUEOUS FERMENTATIVE LIQUID | 01-14-2016 |
| 20160010121 | COOLING AND PROCESSING MATERIALS | 01-14-2016 |
| 20160017378 | Process for producing lower alkyl alcohols from cellulosic biomass using microorganisms - At least one isolated microorganism, which converts at least 10% by weight, and preferably 50% by weight, of cellulosic biomass to a lower alkyl alcohol by direct digestion, and which produces at least 4% by volume of the lower alkyl alcohol in an aqueous-based digestion medium. | 01-21-2016 |
| 20160024532 | ATP DRIVEN DIRECT PHOTOSYNTHETIC PRODUCTION OF FUELS AND CHEMICALS - Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-l-butanol, 3-methyl-1-butanol and 2-phenylethanol from a suitable substrate. | 01-28-2016 |
| 20160046966 | METHODS AND SYSTEMS FOR PRODUCING FERMENTATION PRODUCTS FROM CARBOHYDRATE-RICH SUBSTRATES - A method for producing fermentation products from lignocellulosic biomass is provided. Lignocellulosic biomass is composed of lignocellulosic fibers which are hollow and primarily contain cellulose, hemicellulose and lignin. Lignin is concentrated in the outer fiber wall and glues the fibers into bundles, but the inner fiber wall has a much lower concentration of lignin and has more easily accessible cellulose and hemicellulose. This method uses vacuum infusion to infuse enzymes into the lumen (hollow center) of lignocellulosic fibers to hydrolyze the hemicellulose and cellulose to produce sugars and oligomers, and then uses cycles of vacuum pressure to pump these homogeneous reagents and sugars and oligomers into and out of the lumen. These reagents are homogenized by mixing the reagents with process water using turbulent mixing to produce a homogeneous reagent. The sugars may be fermented, such as with yeast, to a fermentation product, such as ethanol or butanol. | 02-18-2016 |
| 20160053285 | GENETICALLY MODIFIED CLOSTRIDIUM SACCHAROPERBUTYLACETONICUM - The present invention provides a means for producing butanol in butanol fermentation with high efficiency. The present invention relates to a genetically modified microorganism of | 02-25-2016 |
| 20160083808 | CONTROLLED FLOCCULATION OF LIGNOCELLULOSIC HYDROLYZATES - A method of flocculating a lignin-containing hydrolyzate solution, comprising adding at least two agents, comprising at least one flocculant, effective for flocculating the hydrolyzate, resulting in a cake having a solids content greater than a solids content of a cake obtained from a hydrolyzate treated with an optimum flocculating amount of the flocculant alone | 03-24-2016 |
| 20160108439 | METHODS FOR CONTROLLING ACETOCLASTIC MICROORGANISMS IN ACETOGENIC SYNGAS FERMENTATION PROCESSES - Methods are disclosed for controlling the population of acetoclastic microorganisms in a process for the bioconversion of gas substrate comprising at least one of CO and a mixture of CO | 04-21-2016 |
| 20160108441 | ISOBUTANOL PRODUCTION USING YEASTS WITH MODIFIED TRANSPORTER EXPRESSION - The present invention relates to recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise one or more modifications resulting in the reduction in the expression and/or activity of an endogenous transporter protein. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 04-21-2016 |
| 20160130611 | Microbial Synthesis Of Aldehydes And Corresponding Alcohols - An improved process for alcohol production includes microbial fermentation using a genetically modified microorganism to produce substantial quantities of aldehydes that are stripped from the fermentation medium and condensed. So produced aldehydes are converted in an ex vivo process to corresponding alcohols. | 05-12-2016 |
| 20160130612 | Integration of a Polynucleotide Encoding a Polypeptide that Catalyzes Pyruvate to Acetolactate Conversion - The invention relates to recombinant host cells having at least one integrated polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway, e.g., pyruvate to acetolactate conversion. The invention also relates to methods of increasing the biosynthetic production of isobutanol, 2,3-butanediol, 2-butanol or 2-butanone using such host cells. | 05-12-2016 |
| 20160138049 | OXYGEN-TOLERANT CoA-ACETYLATING ALDEHYDE DEHYDROGENASE CONTAINING PATHWAY FOR BIOFUEL PRODUCTION - Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from a suitable substrate. | 05-19-2016 |
| 20160138050 | YEAST WITH INCREASED BUTANOL TOLERANCE INVOLVING CELL WALL PROTEINS - Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products from a pyruvate utilizing pathway. The yeast host cells provided herein comprise at least one genetic modification in a pyruvate decarboxylase gene and at least one genetic modification in an endogenous cell wall protein, which confers resistance to butanol and increased glucose utilization. | 05-19-2016 |
| 20160138051 | ACETOLACTATE SYNTHASE HAVING 2-KETOISOVALERATE DECARBOXYLASE ACTIVITY AND USES THEREOF - Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and 2-phenylethanol from a suitable substrate and a recombinant acetolactate synthase having both synthase and decarboxylase activity. | 05-19-2016 |
| 20160160242 | METHOD OF PRODUCING ALCOHOL FROM CELLULOSE-CONTAINING BIOMASS - A method of producing alcohol from cellulose-containing biomass includes steps (1) to (8): step (1): pretreating a cellulose-containing biomass; step (2): saccharifying the pretreated cellulose-containing biomass obtained in step (1) with a saccharification enzyme; step (3): removing a saccharification residual solid from a saccharification treated product obtained in step (2); step (4): culturing an alcohol fermentation microorganism with an aqueous sugar solution obtained in step (3) as a fermentation raw material; step (5): removing the alcohol fermentation microorganism from a culture liquid containing the alcohol fermentation microorganism obtained in step (4); step (6): distilling the alcohol fermentation liquid obtained in step (5) to recover alcohol; step (7): passing and filtering a distillation residual liquid obtained in step (6) through a reverse osmosis membrane; and step (8): performing treatment of discharging a retentate obtained in step (7). | 06-09-2016 |
| 20160168597 | Process for the Bioconversion of Butane to 1-Butanol | 06-16-2016 |
| 20160201093 | Process for the Bioconversion of C3-C13 Alkanes to C3-C13 Primary Alcohols | 07-14-2016 |
| 20160376613 | Glycerol 3-Phosphate Dehydrogenase for Butanol Production - Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased K | 12-29-2016 |
| 20170233768 | IMPROVED BATCH TIME IN FERMENTATION PROCESSES | 08-17-2017 |
| 20170233770 | PROCESS TO PRODUCE ORGANIC COMPOUNDS FROM SYNTHESIS GASES | 08-17-2017 |
| 20170233771 | YEAST PREPARATIONS AND METHODS OF MAKING THE SAME | 08-17-2017 |
