| Entries |
| Document | Title | Date |
|---|
| 20080293112 | SUCCINIC ACID-PRODUCING BACTERIUM AND PROCESS FOR PRODUCING SUCCINIC ACID - Coryneform bacterium is modified so that an activity of acetyl-CoA hydrolase is decreased, and succinic acid is produced by using the bacterium. | 11-27-2008 |
| 20080293113 | PROCESS FOR PRODUCTION OF SUCCINIC ACID - Disclosed is a process for production of succinic acid, which comprises the step of reacting a bacterium which has been modified so as to increase the expression of a sucE1 gene or a product produced by any treatment of the bacterium with an organic raw material in a reaction solution containing a carbonate ion, a bicarbonate ion or carbon dioxide gas to thereby yield the desired succinic acid. | 11-27-2008 |
| 20080305533 | Recombinant Microorganisms For Increased Production of Organic Acids - Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant | 12-11-2008 |
| 20090075352 | Method For Improving A Strain Based On In-Silico Analysis - The present invention is related to a method for improving a strain on the basis of in silico analysis, in which it compares the genomic information of a target strain for producing a useful substance to the genomic information of a strain overproducing the useful substance so as to primarily screen genes unnecessary for the overproduction of the useful substance, and then to secondarily screen genes to be deleted through performing simulation with metabolic flux analysis. According to the present invention, an improved strain can be effectively constructed by the metabolic and genetic engineering approach comprising comparatively analyzing the genomic information of a target strain for producing a useful substance and the genomic information of a strain producing a large amount of the useful substance to screen candidate genes and performing in silico simulation on the screened candidate genes to select a combination of genes to be deleted, which shows an improvement in the production of the useful substance. Accordingly, the time, effort and cost required for an actual wet test can be significantly reduced. | 03-19-2009 |
| 20090162910 | METHOD FOR MASS PRODUCTION OF PRIMARY METABOLITES, STRAIN FOR MASS PRODUCTION OF PRIMARY METABOLITES, AND METHOD FOR PREPARATION THEREOF - The present invention relates to a method for mass production of other primary metabolites by inhibiting a specific metabolite of metabolism in microorganisms, a transformant for mass production of other primary metabolites plasmid clone by modifying a specific gene relating to the metabolism, and a method for preparation thereof. The primary metabolites can contain lactate, succinate, or alcohol as ethanol, wherein each has a high industrial applicability as an environmental friendly plasmid clone biochemical material. | 06-25-2009 |
| 20090203095 | NOVEL ENGINEERED MICROORGANISM PRODUCING HOMO-SUCCINIC ACID AND METHOD FOR PREPARING SUCCINIC ACID USING THE SAME - The present invention relates to a mutant microorganism, which is selected from the group consisting of genus | 08-13-2009 |
| 20100041115 | PRODUCTION OF DICARBOXYLIC ACIDS BY IMPROVED MUTANT STRAINS OF YARROWIA LIPOLYTICA - The invention concerns a method for producing dicarboxylic acids (DCA) with long hydrocarbon chains, also called diacids, which consists in culturing a mutant strain of | 02-18-2010 |
| 20100068774 | METHOD FOR PRODUCING AN ORGANIC ACID - An organic acid is produced by allowing a bacterium which has an ability to produce an organic acid and has been modified so that expression of the sucE1 and mdh genes are enhanced, or a product obtained by processing the bacterium, to act on an organic raw material in a reaction mixture containing carbonate ions, bicarbonate ions, or carbon dioxide gas, and collecting the organic acid. | 03-18-2010 |
| 20100075388 | PLASMID, TRANSFORMANTS AND PROCESS FOR PRODUCTION OF 3- CARBOXYMUCONOLACTONE - There is provided a process for industrial production of simple 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone from low molecular mixtures derived from plant components such as vanillin, vanillic acid and protocatechuic acid, via a multistage enzyme reaction. | 03-25-2010 |
| 20100081180 | METHOD FOR PRODUCING AN ORGANIC ACID - An organic acid is produced by allowing a bacterium which has an ability to produce an organic acid and has been modified so that expression of yidE gene is enhanced, or a product obtained by processing the bacterium, to act on an organic raw material in a reaction mixture containing carbonate ions, bicarbonate ions, or carbon dioxide gas to produce the organic acid, and collecting the organic acid. | 04-01-2010 |
| 20100159542 | BACTERIAL CELLS EXHIBITING FORMATE DEHYDROGENASE ACTIVITY FOR THE MANUFACTURE OF SUCCINIC ACID - The present invention relates to a bacterial cell of the genus | 06-24-2010 |
| 20100159543 | BACTERIAL CELLS HAVING A GLYOXYLATE SHUNT FOR THE MANUFACTURE OF SUCCINIC ACID - The present invention is concerned with bacteria for the production of succinic acid. Specifically, the invention relates to a bacterial cell of the genus | 06-24-2010 |
| 20100159544 | HOMO-SUCCINIC ACID PRODUCING MICROORGANISM VARIANT AND PROCESS FOR PREPARING SUCCINIC ACID USING THE SAME - The present invention relates to microbial variants producing homo-succinic acid at high yields and a method for producing homo-succinic acid using the same, more particularly, to a microbial variant constructed by disrupting a lactate dehydro-genase-encoding gene (idhA) and an acetate kinase-encoding gene (ackA), as well as a method for producing homo-succinic acid at high concentration, which comprises culturing such variants using glucose as a carbon source in anaerobic conditions. | 06-24-2010 |
| 20100184171 | Materials and Methods for Efficient Succinate and Malate Production - Genetically engineered microorganisms have been constructed to produce succinate and malate in mineral salt media in pH-controlled batch fermentations without the addition of plasmids or foreign genes. The subject invention also provides methods of producing succinate and malate comprising the culture of genetically modified microorganisms. | 07-22-2010 |
| 20100285546 | Genetically Modified Microorganisms for Producing Itaconic Acid with High Yields - Genetically modified microorganisms that produce itaconic acid at high yields and uses thereof. | 11-11-2010 |
| 20100297715 | METHOD FOR PRODUCING SUCCINIC ACID - The invention relates to methods for producing succinic acid and/or succinate ions by fermentation under anaerobic conditions. | 11-25-2010 |
| 20100297716 | METHOD FOR PRODUCING AN ORGANIC ACID - An organic acid is produced by allowing a bacterium belonging to the family Enterobacteriaceae, which has an ability to produce an organic acid and has been modified so that the phosphoenolpyruvate carboxykinase activity is enhanced, which is selected from | 11-25-2010 |
| 20100311132 | PRODUCTION OF ITACONIC ACID - The invention relates to the production of itaconic acid in micro-organisms by introducting into a suitable host cell a gene coding for the enzyme cis-aconitic acid decarboxylase, preferably derived from | 12-09-2010 |
| 20100330631 | Cis-aconitate Decarboxylase Mutants Having Improved Enzymatic Activity - Cis-aconitate decarboxylase mutants having one or more mutations in a C-terminal region as compared with a wild-type cis-aconitate decarboxylase of | 12-30-2010 |
| 20100330632 | Cis-aconitate Decarboxylase Mutants Having Improved Enzymatic Activity - Cis-aconitate decarboxylase mutants having one or more mutations in a C-terminal region as compared with a wild-type cis-aconitate decarboxylase of | 12-30-2010 |
| 20110045559 | MALIC ACID PRODUCTION IN RECOMBINANT YEAST - The present disclosure relates to modified yeast, wherein the yeast has reduced pyruvate decarboxylase polypeptide (PDC) activity and methods of using such yeast to produce malic and/or succinic acid. | 02-24-2011 |
| 20110053233 | Methods for improving malic acid production in filamentous fungi - The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants. | 03-03-2011 |
| 20110104771 | PROCESS FOR THE PRODUCTION OF A DICARBOXYLIC ACID - The present invention relates to a process for the production of a dicarboxylic acid wherein a eukaryotic cell is fermented in a suitable fermentation medium. The invention further relates to a eukaryotic cell comprising a nucleotide sequence encoding an enzyme which catalyses the conversion of isocitric acid to succinic acid, and a nucleotide sequence encoding an enzyme which catalyses the conversion of glyoxylic acid to malic acid. | 05-05-2011 |
| 20110124066 | PRODUCTION OF ITACONIC ACID - The invention relates to a nucleic acid sequence encoding an | 05-26-2011 |
| 20110143405 | DICARBOXYLIC ACID PRODUCTION IN A RECOMBINANT YEAST - The present invention relates to a recombinant yeast comprising a nucleotide sequence encoding a heterologous enzyme that catalyses the conversion of malic acid to fumaric acid. The invention further relates to a process for the production of a dicarboxylic acid wherein the yeast according to the present invention is used. | 06-16-2011 |
| 20110177570 | BACTERIAL MUTANTS FOR ENHANCED SUCCINATE PRODUCTION - The present invention relates to a method for obtaining enhanced metabolite production in micro-organisms, and to mutants and/or transformants obtained with said method. More particularly, it relates to bacterial mutants and/or transformants for enhanced succinate production, especially mutants and/or transformants that are affected in the import and export of succinate. | 07-21-2011 |
| 20110201069 | Method for Producing Oligomer and/or Monomer by Degrading Biodegradable Resin - An object of the present invention is to provide a method for efficiently producing an oligomer or a monomer by degrading a biodegradable resin using an enzyme, so that the oligomer or the monomer can be recovered. | 08-18-2011 |
| 20110229945 | DICARBOXYLIC ACID PRODUCTION BY FERMENTATION AT LOW PH - The present invention relates to a process for the production of a dicarboxylic acid. The process comprises fermenting a yeast in the presence of a carbohydrate-containing substrate and low amounts of oxygen at a pH value at which at least 50% of the dicarboxylic acid is in the acid form. The process of the present invention allows for high yields of the dicarboxylic acid product and is more cost-effective than existing processes in which the salt is produced which during recovery has to be converted to the acid. It also leads to a simpler and more convenient downstream processing. | 09-22-2011 |
| 20110244534 | PROCESS FOR THE PREPARATION OF A MONOVALENT SUCCINATE SALT - A process for the preparation of a monovalent succinate salt includes:
| 10-06-2011 |
| 20110300595 | MICROORGANISM FOR PRODUCING SUCCINIC ACID - The invention relates to an isolated, genetically modified microorganism, wherein compared to the wild type a) the idh1 and idp1 genes have been deleted or inactivated, and/or b) the sdh2 and sdh1 genes have been deleted or inactivated, and/or c) the PDC2 gene has been deleted or inactivated or is under the control of a promoter which can be suppressed or induced by exposure of the microorganism using an inductor substance, and/or d) one or more genes from the group consisting of ICL1, MLS1, ACS1 and MDH3 has been replaced or supplemented by a corresponding foreign gene or corresponding foreign genes from Crabtree-negative organisms, and to the uses thereof. | 12-08-2011 |
| 20110312047 | Polypeptides having C4 Dicarboxylic Acid Transporter Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having C4-dicarboxylic acid transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid. | 12-22-2011 |
| 20110318795 | DICARBOXYLIC ACID PRODUCTION IN A FILAMENTOUS FUNGUS - The present invention relates to a recombinant fungus comprising an enzyme which catalyses the conversion of malic acid to fumaric acid in the cytosol. The invention further relates to a process for the production of a dicarboxylic acid such as fumaric acid and succinic acid, wherein the recombinant fungus is used. | 12-29-2011 |
| 20120003708 | METHODS OF PRODUCING C4 DICARBOXYLIC ACIDS - Methods of producing C4 dicarboxylic acids are disclosed. Nucleotide sequences encoding pyruvate carboxylase and uses of such nucleotide sequences in the production of C4 dicarboxylic acids are further disclosed. | 01-05-2012 |
| 20120015415 | METHOD FOR PRODUCING SUCCINIC ACID USING A YEAST BELONGING TO THE GENUS Yarrowia - The present invention provides a method for producing succinic acid using a yeast belonging to the genus | 01-19-2012 |
| 20120021473 | PROCESSES FOR PRODUCING CARBOXYLIC ACIDS FROM FERMENTATION BROTHS CONTAINING THEIR AMMONIUM SALTS - Processes for making SA from either a clarified DAS-containing fermentation broth or a clarified MAS-containing fermentation broth that include distilling the broth under super atmospheric pressure at a temperature of >100° C. to about 300° C. to form an overhead that comprises water and ammonia, and a liquid bottoms that includes SA, and at least about 20 wt % water; cooling the bottoms to a temperature sufficient to cause the bottoms to separate into a liquid portion and a solid portion that is substantially pure SA; and separating the solid portion from the liquid portion. A method also reduces the broth distillation temperature and pressure by adding an ammonia separating and/or water azeotroping solvent to the broth. | 01-26-2012 |
| 20120021474 | BIOLOGICAL METHODS FOR PREPARING ADIPIC ACID - The technology relates in part to biological methods for producing adipic acid and engineered microorganisms capable of such production. | 01-26-2012 |
| 20120040422 | DICARBOXYLIC ACID PRODUCTION PROCESS - The present invention relates to a process for the production of a dicarboxylic acid and ethanol which comprises fermenting a genetically modified yeast in a suitable fermentation medium under anaerobic conditions at a pH value of between 1 and 5 and producing the dicarboxylic acid and ethanol. The invention also relates to a process for crystallizing succinic acid from an aqueous solution having a pH of between 1 and 5 and comprising succinic acid, comprising removing part of the aqueous solution by evaporation to obtain a concentrated solution, and bringing the temperature of the concentrated solution to a value of between 10 and 30 degrees Celsius, wherein succinic acid crystals are formed | 02-16-2012 |
| 20120040423 | Fermentation - The present invention provides a method for providing a target chemical, comprising: providing a cellulose-derived slurry comprising fermentable saccharides in one or several containers; subjecting cellulose-derived slurry from said one or several containers to separation to provide a liquid fraction comprising part of said fermentable saccharides from said cellulose-derived slurry and a suspended solids fraction, which is returned to said container; transferring said liquid fraction to a fermentor; fermenting fermentable saccharides of said liquid fraction in said fermentor to provide a fermented liquid comprising said target chemical; and returning said fermented liquid to said one or several containers. | 02-16-2012 |
| 20120058530 | ENGINEERING THE PATHWAY FOR SUCCINATE PRODUCTION - This invention relates to the biocatalysts for the efficient production of succinic acid and/or other products from renewable biological feedstocks. The biocatalysts have a very high efficiency for the growth-coupled production of succinic acid and/or other products from carbohydrate feed stocks as a result of both genetic manipulations and metabolic evolution. More specifically, certain biocatalysts of the present invention produce succinic acid at high titers and yield in mineral salts media during simple pH-controlled, batch fermentation without the addition of any exogenous genetic material. The genetic manipulations of the present invention are concerned with the energy-conserving strategies coupled with the elimination of alternative routes for NADH oxidation other than the routes for succinic acid production. The biocatalysts contain glucose-repressed gluconeogenic phosphoenol pyruvate carboxykinase (pck) depressed by genetic modifications and a genetically-inactivated phosphotransferase system. In terms of succinic acid production efficiency, the biocatalysts of the present invention are functionally equivalent to succinate producing rumen bacteria such as | 03-08-2012 |
| 20120129233 | METHOD FOR PRODUCING AN ORGANIC ACID - An organic acid is produced by allowing a bacterium belonging to the family Enterobacteriaceae, which has an ability to produce an organic acid and has been modified so that the phosphoenolpyruvate carboxykinase activity is enhanced, and the glucose phosphotransferase activity is decreased, which is selected from | 05-24-2012 |
| 20120149079 | Polypeptides Having C4 Dicarboxylic Acid Transporter Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having C4-dicarboxylic acid transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid. | 06-14-2012 |
| 20120164703 | RECOMBINANT MICROORGANISMS FOR INCREASED PRODUCTION OF ORGANIC ACIDS - Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinant | 06-28-2012 |
| 20120219999 | Microorganisms for C4-Dicarboxylic Acid Production - The present invention relates to isolated polypeptides having bicarbonate transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid. | 08-30-2012 |
| 20120220000 | ENGINEERING MICROBES FOR EFFICIENT PRODUCTION OF CHEMICALS - This present invention relates to production of chemicals from microorganisms that have been genetically engineered and metabolically evolved. Improvements in chemical production have been established, and particular mutations that lead to those improvements have been identified. Specific examples are given in the identification of mutations that occurred during the metabolic evolution of a bacterial strain genetically engineered to produce succinic acid. This present invention also provides a method for evaluating the industrial applicability of mutations that were selected during the metabolic evolution for increased succinic acid production. This present invention further provides microorganisms engineered to have mutations that are selected during metabolic evolution and contribute to improved production of succinic acid, other organic acids and other chemicals of commercial interest. | 08-30-2012 |
| 20120225461 | ORGANIC ACID PRODUCTION IN MICROORGANISMS BY COMBINED REDUCTIVE AND OXIDATIVE TRICABOXYLIC ACID CYLCE PATHWAYS - This invention relates to succinic acid production from renewable feedstock using microbial biocatalysts genetically modified to produce succinic acid in commercially significant quantities. More specifically, this invention relates to the genetic manipulations in the pathway of carbon from renewable feedstock to succinic acid. | 09-06-2012 |
| 20130052705 | ENGINEERED BACTERIA PRODUCE SUCCINATE FROM SUCROSE - Bacteria optimized to produce succinate and other feedstocks by growing on low cost carbon sources, such as sucrose. | 02-28-2013 |
| 20130059353 | Recombinant Microorganisms for Production C4-Dicarboxylic Acids - Provided herein are host cells comprising carbonic anhydrase activity, wherein the cells are capable of producing C4-dicarboxylic acid. Also provided are methods of producing C4-dicarboxylic acid comprising (a) cultivating the host cells having carbonic anhydrase activity in a medium under suitable conditions to produce C4-dicarboxylic acid; and (b) recovering the C4-dicarboxylic acid. | 03-07-2013 |
| 20130089902 | C4 DICARBOXYLIC ACID PRODUCTION IN FILAMENTOUS FUNGI - The present invention relates to methods of producing C4 dicarboxylic acids, such as malic acid, comprising: (a) cultivating a host cell comprising a polynucleotide encoding a C4 dicarboxylic acid transporter; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, as well as host cells comprising the polynucleotides. | 04-11-2013 |
| 20130130339 | FERMENTATION PROCESS FOR THE PRODUCTION OF ORGANIC ACIDS - This invention relates to improvements in the fermentation process used in the production of organic acids from biological feedstock using bacterial catalysts. The improvements in the fermentation process involve providing a fermentation medium comprising an appropriate form of inorganic carbon, an appropriate amount of aeration and a biocatalyst with an enhanced ability to uptake and assimilate the inorganic carbon into the organic acids. This invention also provides, as a part of an integrated fermentation facility, a novel process for producing a solid source of inorganic carbon by sequestering carbon released from the fermentation in an alkali solution. | 05-23-2013 |
| 20130143289 | Pre-Treatment of Cellulosic Material - A method of pre-treating a cellulosic material before hydrolysis is provided. The method comprises the steps of: impregnating the cellulosic material with a reactive water-soluble gas, such as sulphur dioxide (SO | 06-06-2013 |
| 20130157330 | L-MALATE PRODUCTION BY METABOLICALLY ENGINEERED ESCHERICHIA COLI - A process for the production of malic acid in commercially significant quantities from the carbon compounds by genetically modified bacterial strains (GMBS; also referred to as biocatalysts or genetically modified microorganisms) is disclosed. Microorganisms suitable for the production of malic acid can be cultured in one or two-step processes as disclosed herein. | 06-20-2013 |
| 20130203137 | INCREASING BACTERIAL SUCCINATE PRODUCTIVITY - Improved bacteria for making succinate and other 4 carbon dicarboxylates from the Krebs cycle have modifications to reduce acetate, lactate, EtOH and formate, as well as turn on the glyoxylate shunt, produce more NADH and overexpress In one embodiment, the bacteria are ΔadhEΔldhAΔiclRΔack-pta plus PYC | 08-08-2013 |
| 20130217087 | NOVEL MUTANT MICROORGANISM PRODUCING SUCCINIC ACID SIMULTANEOUSLY USING SUCROSE AND GLYCEROL, AND METHOD FOR PREPARING SUCCINIC ACID USING SAME - The present invention relates to a succinic acid-producing mutant microorganism that is able to utilize sucrose and glycerol simultaneously as carbon sources. More particularly, the present invention relates to a succinic acid-producing mutant microorganism that is able to utilize sucrose and glycerol simultaneously for succinic acid production, the mutant organism being obtained by relieving the mechanism of sucrose-mediated catabolite repression of glycerol in a succinic acid-producing microorganism. | 08-22-2013 |
| 20130266998 | NOVEL OXIDOREDUCTASES FOR ENANTIOSELECTIVE REACTIONS - Described herein are compositions and methods for generating oxidoreductases for enantioselective reactions. Described herein are compositions and methods for generating neomorphic (R)-2-hydroxyacid dehydrogenases capable of enzymatically converting a 1-carboxy-2-ketoacid to a 1-carboxy-(R)-2-hydroxyacid, or the reverse reaction. Illustrative examples include (a) (R)-2-hydroxyadipate dehydrogenase and uses thereof for converting 2-oxoadipate to (R)-2-hydroxyadipate, or the reverse reaction; and (b) (R)-2-hydroxyglutarate dehydrogenase and uses thereof for converting 2-oxoglutarate to (R)-2-hydroxyglutarate, or the reverse reaction. Also described herein are compositions and methods for generating non-natural microbial organisms to enzymatically convert 2-oxoadipate to (E)-2-hexenedioate or adipate, or to enzymatically convert 2-oxoglutarate to (E)-2-pentenedioate or glutarate, or the respective reverse reactions. | 10-10-2013 |
| 20130288321 | Methods for Improved C4-Dicarboxylic Acid Production in Filamentous Fungi - The present invention relates to methods of producing C4-dicarboxylic acids, such as malic acid, comprising: (a) cultivating a host cell comprising a polynucleotide encoding a C4-dicarboxylic acid transporter; and (b) recovering the C4-dicarboxylic acid. The present invention also relates to methods for increasing C4-dicarboxylic acid production, as well as host cells comprising the polynucleotides. | 10-31-2013 |
| 20130288322 | Method for Degrading Biodegradable Resin - A method for degrading a readily degradable resin composition comprising an aliphatic polyester (A) which is biodegradable, and an aliphatic polyester (B′) which releases an acid upon hydrolysis and which is biodegradable at a higher degradation rate than that of the aliphatic polyester (A), the method comprising degrading the readily degradable resin composition in an enzyme reaction liquid containing a degradation enzyme, and an acid neutralizing agent incompatible with the enzyme reaction liquid. | 10-31-2013 |
| 20130302865 | Polypeptides Having C4 Dicarboxylic Acid Transporter Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having C4-dicarboxylic acid transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid. | 11-14-2013 |
| 20130302866 | COMPOSITIONS AND METHODS FOR SUCCINATE PRODUCTION - The present application provides genetically modified yeast cell comprising an active succinate fermentation pathway, as well as methods of using these cells to produce succinate. | 11-14-2013 |
| 20130309736 | COMPOSITIONS AND METHODS FOR MALATE AND FUMARATE PRODUCTION - The present application provides genetically modified yeast cell comprising an active malate fermentation pathway and/or an active fumarate fermentation pathway, as well as methods of using these cells to produce malate and/or fumarate. | 11-21-2013 |
| 20130337519 | METHOD OF PRODUCING SUCCINIC ACID AND OTHER CHEMICALS USING SUCROSE-CONTAINING FEEDSTOCK - This invention relates to the production of chemicals by fermentation with a microorganism in which the fermentation medium contains the sugar sucrose. As a specific example, succinic acid is produced from a sucrose-containing renewable feedstock through fermentation using a biocatalyst. Examples of such a biocatalyst include microorganisms that have been enhanced in their ability to utilize sucrose as a carbon and energy source. The biocatalysts of the present invention are derived from the genetic manipulation of parental strains that were originally constructed with the goal to produce one or more chemicals (for example succinic acid and/or a salt of succinic acid) at a commercial scale using feedstocks other than sucrose. The genetic manipulations of the present invention involve the introduction of exogenous genes involved in the transport and metabolism of sucrose into the parental strains. The genes involved in the transport and metabolism of sucrose can also be introduced into a microorganism prior to developing the organism to produce a particular chemical. The genes involved in the transport and metabolism of sucrose can also be used to augment or improve the sucrose transport and metabolism by strains already known to have some ability for sucrose utilization in biological fermentation. | 12-19-2013 |
| 20140030778 | Bacterial Strain and Process for the Fermentative Production of Organic Acids - The present invention relates to a novel bacterial strain designated DD1, which has the ability to produce organic acids, in particular succinic acid (SA), which was originally isolated from bovine rumen, and is capable of utilizing glycerol as a carbon source; and variant strains derived there from retaining said capability; as well as to methods of producing organic acids, in particular succinic acid by making use of said microorganism. | 01-30-2014 |
| 20140045230 | DICARBOXYLIC ACID PRODUCTION IN A FILAMENTOUS FUNGUS - The present invention relates to a recombinant fungus comprising an enzyme which catalysts the conversion of malic acid to fumaric acid in the cytosol. The invention further relates to a process for the production of a dicarboxylic acid such as fumaric acid and succinic acid, wherein the recombinant fungus is used. | 02-13-2014 |
| 20140065680 | DICARBOXYLIC ACID PRODUCTION IN A RECOMBINANT YEAST - The present invention relates to a recombinant yeast comprising a nucleotide sequence encoding a heterologous enzyme that catalyses the conversion of malic acid to fumaric acid. The invention further relates to a process for the production of a dicarboxylic acid wherein the yeast according to the present invention is used. | 03-06-2014 |
| 20140093926 | INTEGRATED SYSTEMS AND METHODS FOR ORGANIC ACID PRODUCTION - Crude bio-based organic acid-producing feedstock is used to produce a bio-based organic acid. Related systems and methods are also described such as an integrated method including a step of producing a crude polyol product and then processing the crude polyol product to produce a bio-based organic acid. | 04-03-2014 |
| 20140127767 | METHODS FOR PREPARATION OF AMMONIUM SALTS OF C4 DIACIDS BY FERMENTAION AND INTEGRATED METHODS FOR MAKING C4 DERIVATIVES - Methods for forming ammonium salts of C4 diacids in a fermentation process with removal of divalent metal carbonate salts are disclosed. The pH of fermentation broths for production of C4 diacids is controlled by adding alkaline oxygen containing calcium or magnesium compounds, which forms divalent metal salts of the diacids. The divalent metal salts of the diacids are substituted with ammonium by introduction of ammonium salts at elevated temperature and pressure forming soluble ammonium salts thereof. C02 or bicarbonate is simultaneously added to the fermentation media at the elevated temperature and pressure. Reducing the temperature and pressure forms insoluble divalent metal carbonate salts that are separated from the solubilized ammonium diacid salts. The recovered carbonate salts can be recycled as pH control materials in subsequent fermentations. The solubilized ammonium diacid salts may form the derivatives N-methyl-2-pyrrolidone (NMP) gamma-butyrolactone (GBL) and 1,4-butane-diol (BDO) in single pot reactions. | 05-08-2014 |
| 20140134687 | MODIFIED MICROORGANISMS AND METHODS OF USING SAME FOR PRODUCING BUTADIENE AND SUCCINATE - The present disclosure generally relates to microorganisms (e.g., non-naturally occurring microorganisms) that comprise one or more polynucleotides coding for enzymes in a pathway that catalyze a conversion of a carbon source (e.g., a fermentable carbon source) to butadiene and succinate and the use of such microorganisms for the production of butadiene and succinate. | 05-15-2014 |
| 20140134688 | Microorganisms for C4-Dicarboxylic Acid Production - The present invention relates to isolated polypeptides having bicarbonate transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid. | 05-15-2014 |
| 20140234925 | Methods for improving malic acid production in filamentous fungi - The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants. | 08-21-2014 |
| 20140295509 | Pre-Treatment of Cellulosic Material - A method of pre-treating a cellulosic material before hydrolysis is provided. The method comprises the steps of: impregnating the cellulosic material with a reactive water-soluble gas, such as sulphur dioxide (SO | 10-02-2014 |
| 20140342416 | PROCESS FOR PREPARING DICARBOXYLIC ACIDS EMPLOYING FUNGAL CELLS - The present invention relates to a process for producing a dicarboxylic acid comprising fermenting a fungal cell in a vessel comprising a suitable fermentation medium, comprising adding a gas flow which comprises 20 to 35 v/v % of oxygen and less than 0.1v/v % of carbon dioxide to the fermentation medium, and maintaining an average partial carbon dioxide pressure of at least about 0.35 bar in the fermentation medium, and producing the dicarboxylic acid. | 11-20-2014 |
| 20140349356 | METHOD FOR REUSING WATER IN FERMENTED BUTANEDIOIC ACID SEPARATION PROCESS - This invention belongs to the field of biochemical engineering and relates to a method of cyclic utilization of water during separation of succinic acid made by fermentation. This invention uses water from separation process for aerobic growth of | 11-27-2014 |
| 20140363862 | Methods for Succinate Production - Provided herein are genetically modified yeast cells for the production of succinate, methods of making these yeast cells, and methods of using these cells to produce succinate. | 12-11-2014 |
| 20150010974 | Method for Degrading a Readily-degradable Resin Composition - A method for degrading a readily degradable resin composition comprising an aliphatic polyester (A) which is biodegradable, and an aliphatic polyester (B′) which releases an acid upon hydrolysis and which is biodegradable at a higher degradation rate than that of the aliphatic polyester (A), the method comprising degrading the readily degradable resin composition in an enzyme reaction liquid containing a degradation enzyme, and an acid neutralizing agent incompatible with the enzyme reaction liquid. | 01-08-2015 |
| 20150024446 | Methods for improving malic acid production in filamentous fungi - The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants. | 01-22-2015 |
| 20150031100 | COMPOSITIONS AND METHODS FOR CHEMICAL REPORTER VECTORS - Embodiments of the present invention concerns methods and compositions for the construction of a series of vectors containing a chemical sensing module to assess the production of a chemical compound by a microorganism. | 01-29-2015 |
| 20150031101 | BACTERIAL CELL HAVING ENHANCED SUCCINIC ACID PRODUCTION AND A METHOD FOR PRODUCING THE SUCCINIC ACID USING THE SAME - A genetically engineered bacterial cell wherein activity of a pathway in the cell of converting α-ketoglutarate into succinate semialdehyde; or activity of succinyl semialdehyde dehydrogenase in the cell is increased compared to the activity in a non-genetically engineered cell of the same type, and a method of producing succinic acid by using the same. | 01-29-2015 |
| 20150044745 | RECOMBINANT CORYNEBACTERIUM AND A METHOD OF PRODUCING C4 DICARBOXYLIC ACID USING THE SAME - A recombinant | 02-12-2015 |
| 20150064753 | CORYNEBACTERIUM COMPRISING NAD+ DEPENDENT FORMATE DEHYDROGENASE GENE AND METHOD FOR PRODUCING C4 DICARBOXYLIC ACID USING THE SAME | 03-05-2015 |
| 20150104840 | METHOD OF CONTINUOUS FERMENTATION PROCESS FOR SUCCINIC ACID BY MICROBIAL CELLS OF ACTINOBACILLUS SUCCINOGENES - The present invention relates to a continuous fermentation process using | 04-16-2015 |
| 20150147792 | Betaine Enhancement of Fermentation to make C4 Diacids - A method of making a C4 diacid from a sugar source by fermentation with a microorganism is described that includes supplementing the fermentation medium with relatively low amounts of betaine. The use of betaine can substantially reduce or even completely eliminate the need for complex nutrient source such as yeast extract, and eliminate a need for vitamins such as biotin, while maintaining a high level of production of the C4 diacid from a simplified sugar based media. The use of betaine improves yield from sugar, final titer, and rate of production per hour. The method is particularly suitable for microorganisms that otherwise display a dependence on yeast extract for high productivity, more particularly for any bacteria, and most particularly for the production of succinic acid from the bacterium | 05-28-2015 |
| 20150329882 | MICROORGANISM HAVING ENHANCED PRODUCTIVITY OF SUCCINATE AND METHOD OF PRODUCING SUCCINATE USING THE SAME - Provided is a recombinant microorganism comprising an exogenous pyruvate dehydrogenase E1 protein, and/or increased expression of alpha-ketoglutarate dehydrogenase E1. | 11-19-2015 |
| 20150329883 | GENETICALLY ENGINEERED BACTERIAL CELL AND METHOD OF PRODUCING SUCCINIC ACID USING THE SAME - Provided are a genetically engineered bacterial cell and a method of producing succinic acid by using the cell. | 11-19-2015 |
| 20160002677 | STRAIN FOR PRODUCING SUCCINATE FROM CARBON DIOXIDE AND METHOD FOR SUCCINATE PRODUCTION USING THE STRAIN - The present disclosure relates to a strain capable of producing succinate using starch accumulated in microalgae which grow using carbon dioxide as a direct carbon source without converting it to glucose and a method for producing succinate using the same. The present disclosure provides a strain producing succinate from carbon dioxide, selected from a group consisting of | 01-07-2016 |
| 20160017382 | MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF FUMARATE, MALATE, AND ACRYLATE - A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms. | 01-21-2016 |
| 20160068872 | COMPOSITIONS AND METHODS FOR MALATE AND FUMARATE PRODUCTION - The present application provides genetically modified yeast cell comprising an active malate fermentation pathway and/or an active fumarate fermentation pathway, as well as methods of using these cells to produce malate and/or fumarate. | 03-10-2016 |
| 20160083753 | METABOLIC EVOLUTION OF ESCHERICHIA COLI STRAINS THAT PRODUCE ORGANIC ACIDS - This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids. | 03-24-2016 |
| 20160097064 | RECOMBINANT ESCHERICHIA COLI FOR PRODUCING SUCCINIC ACID AND APPLICATION THEREOF - The invention relates to the field of producing succinate by | 04-07-2016 |
| 20160145648 | ESCHERICHIA COLI CONTAINING MUTATED LPDA GENE AND APPLICATION THEREOF - The invention relates to the field of modifying | 05-26-2016 |
| 20160160245 | METHOD OF PRODUCING SUCCINIC ACID AND OTHER CHEMICLAS USING FACILITATED DIFFUSION FOR SUGAR IMPORT - This invention relates to the production of succinic acid and other chemicals derived from phosphoenolpyruvate (PEP) by fermentation with a microorganism in which the fermentation medium contains one or more sugars, and in which one or more of the sugars is imported into the cell by facilitated diffusion. As a specific example, succinic acid is produced from a glucose-containing renewable feedstock through fermentation using a biocatalyst. Examples of such a biocatalyst include microorganisms that have been enhanced in their ability to utilize glucose as a carbon and energy source. The biocatalysts of the present invention are derived from the genetic manipulation of parental strains that were originally constructed with the goal to produce one or more chemicals (for example succinic acid and/or a salt of succinic acid) at a commercial scale using feedstocks that include, for example, glucose, fructose, or sucrose. The genetic manipulations of the present invention involve the introduction of exogenous genes involved in the transport and metabolism of glucose or fructose into the parental strains. The genes involved in the transport and metabolism of glucose or fructose can also be introduced into a microorganism prior to developing the organism to produce a particular chemical. The genes involved in the transport and metabolism of sucrose can also be used to augment or improve the efficiency of sugar transport and metabolism by strains already known to have some ability for glucose utilization in biological fermentations. | 06-09-2016 |
| 20160177345 | RECOMBINANT HOST CELLS FOR THE PRODUCTION OF MALONATE | 06-23-2016 |
| 20160194670 | FERMENTATION PROCESS | 07-07-2016 |
| 20160376616 | TREATMENT METHOD OF SACCHARIDE SOLUTION, PRODUCTION METHOD OF TREATED SACCHARIDE SOLUTION, TREATED SACCHARIDE SOLUTION, PRODUCTION METHOD OF ORGANIC COMPOUND AND CULTURE METHOD OF MICROORGANISM - The invention relates to a method for treating a solution containing a saccharide comprising a heat treatment step of heating the saccharide solution at a temperature of 100° C. or higher and 180° C. or lower and a reduction step of reacting a reducing agent with the saccharide solution heated in the heat treatment step, a method for producing a treated saccharide solution using the treatment method, a treated saccharide solution obtained through treatment by the treatment method, a method for producing an organic compound comprising an organic-matter production step of obtaining an organic compound by causing a microorganism capable of producing organic matter to act on an organic raw material containing the treated saccharide solution and a method for culturing a microorganism using the treated saccharide solution as a carbon source. | 12-29-2016 |
