| Class / Patent application number | Description | Number of patent applications / Date published |
|---|
| 435129000 | Amide (e.g., chloramphenicol, etc.) | 55 |
| 20080248538 | Cyanide-Tolerant Nitrile Hydratases - Disclosed herein are cyanide-tolerant nitrile hydratases especially from | 10-09-2008 |
| 20080261281 | Novel Carbonyl Reductase, Gene Thereof and Method of Using the Same - The present invention relates to a polypeptide having an activity to asymmetrically reduce (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone to produce (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol isolated from a microorganism belonging to the genus | 10-23-2008 |
| 20090011479 | Biotransformation of colchicinoid compounds - The present invention relates to a biotransformation process, effected by means of selected microbial strains, for the preparation of 3-O-glycosyl derivatives of colchicinoid compounds. | 01-08-2009 |
| 20090104671 | METHOD FOR PRODUCING OPTICALLY ACTIVE 2-(N-SUBSTITUTED AMINOMETHYL)-3-HYDROXYBUTYRIC ACID ESTER - The present invention relates to a method for producing optically active 2-(N-substituted aminomethyl)-3-hydroxybutyric acid esters wherein a 2-(N-substituted aminomethyl)-3-oxobutyric acid ester is treated with an enzyme source capable of stereoselectively reducing said ester to the corresponding optically active 2-(N-substituted aminomethyl)-3-hydroxybutyric acid ester having the (2S,3R) configuration. The present invention provides an efficient method for industrially producing optically active 2-(N-substituted aminomethyl)-3-hydroxybutyric acid esters, in particular such compounds having the (2S,3R) configuration, which are useful as intermediates for the production of medicinal compounds, among others. | 04-23-2009 |
| 20090104672 | Novel Microbe, Lipid Modifying Agent, Process for Producing 2-Acyl-Lysophospholipid, Process for Producing Diacylglycerol, Process for Producing Ceramide, and Method of Degumming Oil or Fat - The present invention provides a new supply source of enzymes useful for modification of phospholipids for example, and also provides a method for producing 2-acyl lysophospholipid, a method for producing monoacylglycerol, and a method for producing ceramide, as well as a new method for degumming fat and oil. | 04-23-2009 |
| 20090117628 | Enzymatic processing in deep eutectic solvents - It has surprisingly been discovered that it is possible to use enzymes in deep eutectic solvents (DES). DES's are mixtures of a nitrogen salt or a metal salt and a strong hydrogen bond donor that can be mixed in proportions that form a eutectic point. | 05-07-2009 |
| 20090148917 | METHOD FOR PRODUCING CHIRAL ALCOHOLS - The invention relates to a method for producing an enantiopure alcohol of general formula (Ia) or (Ib), wherein R | 06-11-2009 |
| 20090269822 | MICROORGANISM - A microorganism which is | 10-29-2009 |
| 20090311759 | PROCESS FOR PRODUCING (METH) ACRYLAMIDE - [Problem] To provide a process for producing (meth)acrylamide using a microbial catalyst, wherein production steps can be simplified and production cost can be reduced. | 12-17-2009 |
| 20090325246 | PROCESS FOR PRODUCTION OF BETAINE - According to the present invention, by using 4-halogeno-3-hydroxybutanamide as a substrate in quaternary amination reaction with trialkylamine which is an important step in betaine (such as carnitine) preparation processes, it becomes possible to reduce the production of crotonic acid derivatives (the major by-product) greatly compared to conventional processes. Consequently, it becomes possible to prepare a betaine, such as carnitine, at a high yield. | 12-31-2009 |
| 20100190219 | PRODUCTION OF SPHINGOID BASES USING GENETICALLY ENGINEERED MICROBIAL STRAINS - The present invention provides genetically engineered microbial strains, in particular genetically engineered yeast strains, that produce at least 0.5 mg per g CDW of a sphingoid base according to Formula I or a salt or ester thereof. The present invention provides a method to obtain genetically engineered microbial strains producing at least 0.5 mg per g CDW of a sphingoid base according to Formula I or a salt or ester thereof. The method comprises the steps of: a) increasing the expression of a polynucleotide encoding an enzyme having ceramide synthase activity and/or an enzyme having ceramidase activity, the latter being capable of preferentially, or even specifically, hydrolyzing ceramides containing a sphingoid base according to Formula I, and/or b) decreasing the expression of a polynucleotide encoding an enzyme having sphingolipid Δ8-desaturase activity and/or an enzyme having ceramidase activity, the latter being capable of preferentially, or even specifically, hydrolyzing ceramides containing phytosphingosine or dihydrosphingosine as sphingoid base, and isolating strains with the required productivity. | 07-29-2010 |
| 20100267096 | USE OF A SYNERGISTIC MIXTURE OF WATER-SOLUBLE POLYMERS AND HYDROPHOBINS FOR THICKENING AQUEOUS PHASES - The invention relates to the use of a synergistic mixture of water-soluble polymers with thickening action and hydrophobins for thickening aqueous phases, and to the degradation of the thickening action by cleaving the protein. The invention further relates to a thickening composition of water-soluble polymers, hydrophobins and water. | 10-21-2010 |
| 20100311130 | ENZYMATIC ESTER HYDROLYSIS - The present invention relates to a method for selectively hydrolysing a pendant ester bond formed by an unsubstituted or substituted hydrocarbon group—optionally comprising one or more heteroatoms—and a pendant carboxylate moiety, which carboxylate moiety is part of a polymer or a polymerisable compound, which polymer or polymerisable compound comprises at least one other hydrolysable group, wherein the method comprises contacting the polymer or polymerisable compound with a hydrolytic enzyme. | 12-09-2010 |
| 20110008853 | MANUFACTURE OF AMIDES - A method of producing an amide from the corresponding nitrile comprising the following steps,
| 01-13-2011 |
| 20110039314 | Composition for Catalytic Amide Production and Uses Thereof - A catalytic composition for the enzymatic conversion of nitriles to amides is disclosed. The composition contains a polymer gel and a nitrile hydratase (NHase). Also disclosed are methods of producing an amide from a nitrile using the catalytic composition. | 02-17-2011 |
| 20110097768 | Novel Burkholderia Multivorans LG 31-3, Amidase Produced From the Same, and Method for Resolution of Racemic Mixture Using the Same - The present invention relates to a novel | 04-28-2011 |
| 20110104765 | STEREOSELECTIVE SYNTHESIS OF METYROSINE - Provided herein are compositions including diastereomers in substantially diastereomerically pure form and enantiomers in substantially enantiomerically pure form, and processes for preparing them and converting them to metyrosine. | 05-05-2011 |
| 20110171701 | METHOD FOR PRODUCING ACRYLAMIDE - [Problem] The present invention provides a method for producing acrylamide from acrylonitrile using a biocatalyst with high productivity at low cost, in which reaction heat can be efficiently removed even at an industrial reaction scale. | 07-14-2011 |
| 20110294171 | PREPARATION OF A SATURATED ALDEHYDE - The invention relates to a compound according to Formula (IX) | 12-01-2011 |
| 20110312043 | EXTRACTION SOLVENTS DERIVED FROM OIL FOR ALCOHOL REMOVAL IN EXTRACTIVE FERMENTATION - In an alcohol fermentation process, oil derived from biomass is hydrolyzed into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be catalytically (e.g., enzymatically) hydrolyzed into free fatty acids, which form a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be hydrolyzed by contacting the feedstock including the oil with one or more enzymes whereby at least a portion of the oil is hydrolyzed into free fatty acids forming a fermentation product extractant, or the oil can be separated from the feedstock prior to the feedstock being fed to a fermentation vessel, and the separated oil can be contacted with the enzymes to form the fermentation product extractant. The fermentation product extractant can be contacted with a fermentation broth for in situ removal of a product alcohol. | 12-22-2011 |
| 20110312044 | EXTRACTION SOLVENTS DERIVED FROM OIL FOR ALCOHOL REMOVAL IN EXTRACTIVE FERMENTATION - In an alcohol fermentation process, oil derived from biomass is chemically converted into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be chemically converted into a reaction product, such as fatty acids, fatty alcohols, fatty amides, fatty acid methyl esters, fatty acid glycol esters, and hydroxylated triglycerides, and mixtures thereof, which forms a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be chemically converting into the fermentation product extractant. The oil can be separated from the feedstock prior to the feedstock being fed to a fermentation vessel, and the separated oil can be chemically converted to a fermentation product extractant, which can then contacted with a fermentation product comprising a product alcohol, whereby the product alcohol is separated from the fermentation product. | 12-22-2011 |
| 20120107885 | Anaerobic Fermentation of Glycerol - The invention relates to the development of appropriate cultivation conditions for a bacteria to grow anaerobically (fermentatively) on a glycerol substrate. The method requires culturing bacteria having a functional 1,2-propanediol pathway and a functional type II glycerol dehydrogenase-dihydroxyacetone kinase pathway in a culture medium containing high concentrations of glycerol, a neutral to mildly acidic pH, low levels of potassium and phosphate, and high levels of CO2, such that glycerol is thus converted into a desirable product, such as ethanol, hydrogen, formate, succinate, or 1,2-propanediol. | 05-03-2012 |
| 20120276601 | METHOD FOR PRODUCING ACRYLAMIDE USING MICROBIAL CATALYST - A method for producing acrylamide from acrylonitrile by a biocatalyst method, wherein both evaporation of acrylonitrile into a gas phase and damaging of a catalyst by stirring are prevented, is provided. In the present invention, the production of acrylamide from acrylonitrile by the biocatalyst method comprises feeding acrylonitrile into an aqueous medium comprising a microbial catalyst. | 11-01-2012 |
| 20130059348 | NOVEL AMIDASE, GENE FOR THE SAME, VECTOR, TRANSFORMANT, AND METHOD FOR PRODUCTION OF OPTICALLY ACTIVE CARBOXYLIC ACID AMIDE AND OPTICALLY ACTIVE CARBOXYLIC ACID BY USING ANY ONE OF THOSE ITEMS - The present invention has its object to provide a novel polypeptide having amidase activity to selectively hydrolyze S-enantiomer in racemic nipecotamide, a DNA encoding the polypeptide, a vector containing the DNA, a transformant transformed with the vector, and a method for producing an optically active carboxylic acid amide and an optically active carboxylic acid in which a racemic carboxylic acid amide is hydrolyzed with the polypeptide or the transformant. | 03-07-2013 |
| 20130059349 | METHOD FOR PRODUCING ACRYLAMIDE USING MICROBIAL CATALYST - The present invention provides a more efficient method for producing acrylamide from acrylonitrile by the action of a microbially-derived enzyme, nitrile hydratase. More specifically, the present invention provides a method for producing acrylamide from acrylonitrile using a biocatalyst having nitrile hydratase, which comprises the step of keeping acrylonitrile while cooling to less than 30° C. Moreover, the present invention also provides an apparatus for producing acrylamide from acrylonitrile using a biocatalyst having nitrile hydratase. | 03-07-2013 |
| 20130078683 | Host Cells and Methods for Producing Cinnamoyl Anthranilate and Analogs Thereof - The present invention provides for a method of producing a cinnamoyl anthranilate, or analog thereof, in a genetically modified host cell. | 03-28-2013 |
| 20130095535 | ENZYMATIC RESOLUTION OF RACEMIC (2R,S)-2-(ACETYLAMINO)-3-METHOXY-N-(PHENYLMETHYL)PROPANAMIDE - The present invention is concerned with a process of preparing (R)-lacosamide. The process comprises providing an (R,S)-lacosamide precursor and contacting the same with at least an enzyme in the presence of a solvent. The enzyme either stereoselectively hydrolyzes or acetylates an (R)- or (S)-enantiomer of the (R,S)-lacosamide precursor. The process further comprises where appropriate also concurrently, or successively, employing one or more reagents capable of converting the hydrolysed or acetylated (R)- or (S)-enantiomer to (R)-lacosamide. | 04-18-2013 |
| 20130217081 | BIOCATALYTIC METHODS TO CONVERT CYCLOHEXANE OXIDATION PROCESS WASTE STREAMS TO USEFUL PRODUCTS - The invention relates to methods for enriching monomer content in a cycloalkane oxidation process mixed organic waste stream. In particular, the methods involve combining a biocatalyst with a mixed organic waste stream from a cycloalkane oxidation process, and enzymatically converting dimeric and/or oligomeric components of said waste stream into monomeric components. The methods may enrich the content of diacids, adipic acid, and/or other α,ω-difunctional C6 alkanes in the mixed organic waste stream. Additionally, the treated mixed organic waste streams may have improved burning efficiency. | 08-22-2013 |
| 20130236935 | EXTRACTION SOLVENTS DERIVED FROM OIL FOR ALCOHOL REMOVAL IN EXTRACTIVE FERMENTATION - In an alcohol fermentation process, oil derived from biomass is hydrolyzed into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be catalytically (e.g., enzymatically) hydrolyzed into free fatty acids, which form a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be hydrolyzed by contacting the feedstock including the oil with one or more enzymes whereby at least a portion of the oil is hydrolyzed into free fatty acids forming a fermentation product extractant, or the oil can be separated from the feedstock prior to the feedstock being fed to a fermentation vessel, and the separated oil can be contacted with the enzymes to form the fermentation product extractant. The fermentation product extractant can be contacted with a fermentation broth for in situ removal of a product alcohol. | 09-12-2013 |
| 20140087434 | METHOD FOR PRODUCING AQUEOUS ACRYLAMIDE SOLUTION - There is provided a method for producing an aqueous acrylamide solution by reacting a composition including acrylonitrile with water to produce acrylamide, in which the composition including acrylonitrile includes 3 to 15 mg of propionitrile per 1 kg of the total weight of the composition including acrylonitrile. According to the present invention, a production method with which it is possible to suppress acrylamide polymerization without lowering quality and thereby obtain a stable aqueous acrylamide solution can be provided. | 03-27-2014 |
| 20140106415 | METHOD FOR PRODUCING ACRYLAMIDE AQUEOUS SOLUTION - There is provided a method for producing an aqueous acrylamide solution by producing acrylamide by reacting a composition including acrylonitrile with water, in which the composition including acrylonitrile includes 20 to 80 mg of methacrylonitrile per 1 kg of the total weight of the composition including acrylonitrile. According to the present invention, a production method allowing stable obtainment of an aqueous acrylamide solution can be provided as polymerization of the acrylamide can be suppressed without causing a decreas+455e in quality. | 04-17-2014 |
| 20140120588 | NITRILE HYDRATASE - Improving wild nitrile hydratase enables the provision of a protein which has nitrile hydratase activity and which has further improved heat resistance, amide compound resistance and high temperature accumulation properties. Use protein (A) or (B), (A) being a protein characterised by having nitrile hydratase activity and by including an amino acid sequence in which a specific amino acid residue in an amino acid sequence in wild nitrile hydratase has been substituted by another amino acid residue, and (B) being a protein characterised by having nitrile hydratase activity and by including an amino acid sequence in which one or several amino acid residues in the amino acid sequence of protein (A), other than the abovementioned specific amino acid residue, is deleted, substituted and/or added. | 05-01-2014 |
| 20140134682 | PROCESSES AND RECOMBINANT MICROORGANISMS FOR THE PRODUCTION OF CADAVERINE - Recombinant microorganisms comprising DNA molecules in a deregulated form which improve the production of cadaverine or N-acetylcadaverine, as well as recombinant DNA molecules and polypeptides used to produce the microorganisms are provided. Said microorganisms comprise an intracellular lysine decarboxylase activity and a deregulated cadaverine export activity, or comprise a decreased cadaverine export activity and an enhanced N-acetylcadaverine forming activity. Processes for the production of cadaverine N-acetylcadaverine using the recombinant microorganisms are also provided. | 05-15-2014 |
| 20140134683 | METHOD FOR PRODUCING ACRYLAMIDE - The present invention relates to a method for producing acrylamide comprising: supplying a raw material water to a reactor, supplying acrylonitrile to the reactor, and hydrating acrylonitrile using a biocatalyst, wherein a temperature of the raw material water in the supplying step of the raw material water to the reactor is equal to or more than freezing point of the raw material water and lower than the reaction temperature by 5° C. or more. The present invention can be provided to the method of producing acrylamide which can be removed the reaction heat produced at the hydration reaction of acrylonitrile effectively with a low cost. | 05-15-2014 |
| 20140199737 | Methods Of Producing 6-Carbon Chemicals Via Methyl-Ester Shielded Carbon Chain Elongation - This document describes biochemical pathways for producing adipic acid, 6-aminohexanoic acid, 6-hydroxhexanoic acid, hexamethylenediamine, caprolactam, or 1,6-hexanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the enzymes or homologs accepting methyl ester shielded dicarboxylic acid substrates. | 07-17-2014 |
| 20140220644 | IMPROVED NITRILE HYDRATASE - Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 50 (GX | 08-07-2014 |
| 20140234917 | Enzymatic Preparation of Diols - The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of two ends of a substituted or unsubstituted, linear or branched aliphatic hydrocarbons. | 08-21-2014 |
| 20140234918 | Polypeptides Having Peroxygenase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 08-21-2014 |
| 20140242647 | IN VIVO AND IN VITRO OLEFIN CYCLOPROPANATION CATALYZED BY HEME ENZYMES - The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention. | 08-28-2014 |
| 20140256005 | PROCESS FOR THE PRODUCTION OF GAMMA-AMINOBUTYRIC ACID - The present invention relates to a novel method for the fermentative production of gamma-aminobutyric acid (GABA) by cultivating a recombinant microorganism expressing an enzyme having a glutamate decarboxylase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as to a method for preparing polyamides making use of GABA as obtained fermentative production. | 09-11-2014 |
| 20140273110 | FUNCTIONALIZED CARBOXYLIC ACIDS AND ALCOHOLS BY REVERSE FATTY ACID OXIDATION - Bacteria that run the beta oxidation cycle in reverse anabolic direction are provided, along with many novel primers to start the reverse cycle, pathways to make such primers, and a large variety of products produced thereby. Methods for making desired product by using such primers in the reverse pathway are also disclosed. | 09-18-2014 |
| 20140322769 | BIOCATALYSTS FOR THE PREPARATION OF HYDROXY SUBSTITUTED CARBAMATES - The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides. | 10-30-2014 |
| 20140322770 | Polypeptides Having Peroxygenase Activity And Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having peroxygenaseactivity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 10-30-2014 |
| 20140335575 | NOVEL AMIDASE - An object of the present invention is to provide a means for producing an optically active tropic acid that is a compound useful as a synthetic raw material or an intermediate for pharmaceutical products and the like. The present invention provides a novel polypeptide having activity to (R)-selectively hydrolyze a racemic tropic acid amide, DNA encoding the polypeptide, a vector containing the DNA, a transformant prepared by transformation with the vector, and a method for producing an optically active carboxylic acid amide and an optically active carboxylic acid using them. | 11-13-2014 |
| 20150079643 | MICROBIAL PRODUCTION OF ALKANOLAMIDES AND AMIDOAMINES AND USES THEREOF - The disclosure relates to a recombinant microorganism engineered to express an enzyme which catalyzes the conversion of a primary amine and an acyl thioester to a fatty amide. The disclosure further encompasses a method of producing a fatty amide by culturing the recombinant microorganism in the presence of a carbon source. | 03-19-2015 |
| 20150079644 | Method for Producing 3-Acetylamino-4-Hydroxybenzoic Acid - The present invention provides a method for conveniently and efficiently producing a 3-acetylamino-4-hydroxybenzoic acid-type compound that is a stable compound by a process using a microorganism. Specifically the present invention provides a microorganism having an ability to produce 3-amino-4-hydroxybenzoic acid, that is modified so as to increase an activity to form 3-amino-4-hydroxybenzoic acid from dihydroxyacetone phosphate and aspartate semialdehyde, wherein the microorganism is modified so as to increase an N-hydroxyarylamine O-acetyltransferase (NhoA) activity, as well as a method for producing the 3-acetylamino-4-hydroxybenzoic acid-type compound using such a microorganism. | 03-19-2015 |
| 20150118721 | ACETYLTRANSFERASE FROM WICKERHAMOMYCES CIFERRII - The invention relates to novel enzymes that provide acetylated sphingoid bases. | 04-30-2015 |
| 20150315620 | METHOD FOR PRODUCING ACRYLAMIDE - Provided is a method that is for producing acrylamide, continuously produces acrylamide from acrylonitrile using a biocatalyst, and is characterized in that a continuous reaction is started by means of introducing acrylamide into a reaction vessel and then causing acrylonitrile to contact a biocatalyst. The method for producing acrylamide has superior operability and a low cost, and is capable of continuously extracting an aqueous solution of acrylamide at a target concentration in a short period of time after starting the continuous reaction. | 11-05-2015 |
| 20150376661 | 5-AMINOLEVULINIC ACID HIGH-YIELD BACTERIAL STRAIN, PREPARATION METHOD AND USE THEREOF - A method for constructing an ALA production bacterial strain, the method enhances the activity of related enzymes promoting the synthesis of oxaloacetate and in the 5-aminolevulinic acid (ALA) production bacterial strain, or introducing exogenous related enzymes promoting the synthesis of oxaloacetate, such as phosphoenolpyruvate carboxylase or pyruvate carboxylase, and/or reducing the activity of related enzymes in the downstream metabolic pathway of succinyl coenzyme A in the bacterial strain, such as succinyl coenzyme A synthetase or succinate dehydrogenase, and/or reducing the activity of phosphoenolpyruvate carboxylated kinase and/or malic enzyme. An ALA high-yield bacterial strain constructed by utilizing the method, and method for utilizing the bacterial strain to prepare ALA. | 12-31-2015 |
| 20160002682 | IN VIVO AND IN VITRO OLEFIN CYCLOPROPANATION CATALYZED BY HEME ENZYMES - The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention. | 01-07-2016 |
| 20160002683 | Polypeptides Having Peroxygenase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 01-07-2016 |
| 20160024001 | NOVEL COMPOUNDS - Disclosed are compounds of the general formula I and II (as further defined herein) are useful in the production of inhibitors of sphingolipid synthesis the production of sphingolipids. Suitable sphingolipids, include, but not limited to, sphingosine and compounds incorporating sphingosine or that may use sphingosine as an intermediate or a starting material in their synthesis (including, but not limited to, sphingosine-1-P, ceramide, gangliosides and sphigomyelin). In one contemplated use, compounds of the general formula I and II are useful in the production of sphingosine. In another contemplated use, compounds of the general formula I and II are useful in the production of a sphingofugin. Methods of manufacturing each of the above compounds are also provided. | 01-28-2016 |
| 20160068833 | NITRILE HYDRATASE - Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 50 (GX | 03-10-2016 |
| 20160097046 | NITRILE HYDRATASE - Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 50 (GX | 04-07-2016 |
| 20160160248 | METHOD FOR PRODUCING PULVERULENT CERAMIDE - Disclosed herein is a pulverulent ceramide producing method that enables easily and efficiently extracting and separating ceramide from sugar beet pulp, and efficiently pulverizing the ceramide by spray drying. The pulverulent ceramide can be efficiently obtained by a process that includes concentrating, with and/or without adding water, a sugar beet pulp ethanol extract obtained by extraction of a sugar beet pulp (for example, such as a beet fiber) with ethanol, adding pectinase to the resulting concentrate and performing an enzymatic reaction, performing emulsification after inactivating the enzyme, and pulverizing the resulting emulsion using spray drying. | 06-09-2016 |
