Entries |
Document | Title | Date |
20080299625 | Microbial Strains Producing Sphingoid Bases or Derivatives Thereof - The present invention provides microbial strains, in particular yeast strains, that produce at least 0.1 mg per g biomass dry weight of a sphingoid base. The present invention further provides a method to obtain sphingoid base-producing microbial strains comprising incubating a population of microbial cells in the presence of a suitable concentration of a toxin, selecting cells that are resistant against said toxin, and isolating cells out of the toxin-resistant cell population that produce at least 0.1 mg per g biomass dry weight of the sphingoid base of Formula I. Optionally, the method further comprises subjecting a population of toxin-resistant microbial cells that produce at least 0.1 mg per g biomass dry weight of the sphingoid base of Formula I to DNA-mediated transformation with a polynucleotide encoding an enzyme of the sphingolipid metabolic pathway. The present invention further provides a polypeptide having dihydroceramide desaturase activity obtainable form Pichia ciferrii. | 12-04-2008 |
20080299626 | Process For the Production of an Optically Enriched Tertiary Alcohol - A process for the production of an optically enriched tertiary alcohol of the formula (2a) or (2b), by reacting an epoxide of the formula (1) with a nucleophilic agent Nu in the presence of halohydrin dehalogenase. | 12-04-2008 |
20080305530 | Method for Producing (R)-and (S)-4-(1-Ammoniumethyl)Benzoic Acid Methylester-Sulphate from Racemic 4-(1-Aminoethyl)Benzoic Acid Methylester by Lipase Catalysed Enantoselective Acylation and Subsequent Precipitation with Sulphuric Acid - A method for preparing optically active methyl 4-(1-ammoniumethyl)benzoate sulfate by reacting racemic methyl 4-(1-aminoethyl)benzoate with an acylating agent in the presence of a lipase to give methyl 4-(1-aminoethyl)benzoate and subsequently precipitating methyl 4-(1-ammoniumethyl)benzoate sulfate by adding sulfuric acid. | 12-11-2008 |
20080311635 | PROCESS FOR THE PREPARATION OF (S)(+)-3-(AMINOMETHYL)-5-METHYLHEXANOIC ACID - A process for the preparation of (S)(+)-3-(aminomethyl)-5-methylhexanoic acid (pregabalin) of formula (I) or a salt thereof, | 12-18-2008 |
20090004706 | METHODS TO ENHANCE THE ACTIVITY OF LIGNOCELLULOSE-DEGRADING ENZYMES - Methods for hydrolyzing lignocellulose are provided, comprising contacting the lignocellulose with at least one chemical treatment. Methods for pretreating a lignocellulosic material comprising contacting the material with at least one chemical are also provided. Methods for liberating a substance such as an enzyme, a pharmaceutical, or a nutraceutical from plant material are also provided. These methods are more efficient, more economical, and less toxic than current methods. | 01-01-2009 |
20090011478 | Biochemical Synthesis of 1,4-Butanediamine - The invention relates to a process for biochemical synthesis of 1,4-butanediamine in a microorganism having an increased level of an ornithine decarboxylase activity (increased ODC activity) as compared to the native level of the ornithine decarboxylase activity, wherein in the microorganism also an increased activity of N-acetylglutamate formation is present as compared to the native level of activity of N-acetylglutamate formation in the microorganism and wherein 1,4-butanediamine produced in the microorganism is excreted into a fermentation broth, and is recovered from the fermentation broth. The invention also relates to vectors, plasmids and hosts carrying a corresponding increased ODC activity and an increased activity of N-acetylglutamate formation. | 01-08-2009 |
20090035830 | Method for Preparing Optically Active Amines - The present invention relates to a method of preparing optically active amines and chiral amines prepared thereby. The method includes reacting an amine compound, a metal catalyst, a biocatalyst including a lipase, and an acyl donor compound in an organic solvent to obtain a chiral amide compound, and then hydrolyzing the chiral amide compound to obtain a chiral amine. | 02-05-2009 |
20090042262 | Preparation of Pregabalin and Related Compounds - Materials and Methods for preparing (S)-(+)-3-aminomethyl-5-methyl-hexanoic acid and structurally related compounds via enzymatic kinetic resolution are disclosed. | 02-12-2009 |
20090104670 | N-carbobenzyloxy (N-CBZ)-deprotecting enzyme and uses therefor - This invention relates to isolated or recombinant N-carbobenzyloxy-deprotecting enzyme polypeptides that catalyze the removal of carbobenzyloxy from carbobenzyloxy-protected amino acids and alcohols. Also related are isolated nucleic acids encoding N-carbobenzyloxy-deprotecting enzyme polypeptides thereof, as well as vectors and host cells comprising these nucleic acids. The invention also relates to methods of obtaining isolated nucleic acids, polypeptides, and antibodies, and methods of using the polypeptides in various reactions for industrial or pharmaceutical applications. | 04-23-2009 |
20090117626 | Process for preparing carboxylic acid using surfactant-modified enzyme - The present invention relates to a process for preparing a carboxylic acid using a surfactant-modified enzyme which comprises selectively reacting water and a carboxylic acid ester, provided that triglyceride is excluded, in an organic solvent in the presence of a surfactant-modified enzyme. | 05-07-2009 |
20090117627 | PROCESS FOR PREPARING ENANTIOMERICALLY ENRICHED AMINES - A process for preparing enantiomerically enriched amines by reacting a ketone with ammonia or an ammonium salt and a reducing agent in the presence of a catalytic system comprising the components:
| 05-07-2009 |
20090130726 | PROCESS FOR CONVERTING AROMATIC HALO-SUBSTITUTED DINITRILES INTO HALO-SUBSTITUTED CYANOCARBOXYLIC ACIDS - The present invention is directed to a process for converting aromatic halo-substituted dinitriles into the corresponding cyanocarboxylic acids in the presence of a nitrilase. | 05-21-2009 |
20090233337 | Chemoenzymatic Process for the Stereoselective Preparation of (R)-Gamma-Amino-Beta-Hydroxybutyric Acid [(R)-GABOB) and (R)-Carnitine - The present invention provides a chemoenzymatic process for the preparation of (R)-GABOB and (R)-carnitine employing lipase-mediated resolution of 3-hydroxy-4-tosyloxybutanenitrile as the key step. The drawing accompanying this specification represents the preparation of racemic 3-hydroxy-4-tosyloxybutanenitrile, its lipase-mediated kinetic resolution and its successful application in the preparation of (R)-GABOB and (R)-carnitine. | 09-17-2009 |
20090239273 | NOVEL HIGH-ACTIVITY MODIFIED S-HYDROXYNITRILE LYASE - This invention relates to a novel high-activity modified S-hydroxynitrile lyase (SHNL). More particularly, this invention relates to novel high-activity modified SHNL that is obtained by substituting amino acids at given sites (14, 44, 66, 94, 103, 118, 122, 125, 127, 129, 147, 148, 152, 212, and 216) or inserting amino acid at a given site (between amino acids 128 and 129) of the amino acid sequence (SEQ ID NO: 2) of wild-type SHNL. | 09-24-2009 |
20090246838 | Process For The Production Of Cadaverine - Process for the production of cadaverine by constructing a recombinant microorganism which has a deregulated lysine decarboxylase gene and at least one deregulated gene selected from the group (i) which consists of aspartokinase, aspartatesemialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate succinylase, succinyl-amino-ketopimelate transaminase, succinyl-diamino-pimelate desuccinylase, diaminopimelate epimerase, diaminopimelate dehydrogenase, arginyl-tRNA synthetase, diaminopimelate decarboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxylase, glucose-6-phosphate dehydrogenase, transketolase, transaldolase, 6-phosphogluconolactonase, fructose 1,6-biphosphatase, homoserine dehydrogenase, phophoenolpyruvate carboxykinase, succinyl-CoA synthetase, methylmalonyl-CoA mutase, provided that if aspartokinase is deregulated as gene (i) at least a second gene (i) other than aspartokinase has to be deregulated, and cultivating said microorganism. | 10-01-2009 |
20090275093 | Biochemical Synthesis of 1,4-Butanediamine - The invention relates to a process for biochemical synthesis of 1,4-butanediamine in a microorganism having an increased level of an ornithine decarboxylase activity (increased ODC activity) as compared to the native level of the ornithine decarboxylase activity, wherein the increased ODC activity is obtained by means of overexpression of an ornithine decarboxylase encoding gene with increased translational and/or transcriptional efficiency, and wherein 1,4-butanediamine produced in the microorganism is excreted into a fermentation broth, and is recovered from the fermentation broth. In preferred embodiments also increased enzyme activity is obtained by of overexpression of either (i) an arginine decarboxylase encoding gene speA and an agmatinase encoding gene speB; or (ii) an arginine decarboxylase encoding gene speA and an agmatine iminohydrolase encoding gene aguA, and an N-carbamoylputrescine amidohydrolase encoding gene aguB, and optionally also an agmatinase encoding gene speB. The invention also relates to vectors, plasmids and hosts carrying, at an increased level of activity, one or more of the enzyme activities as mentioned. | 11-05-2009 |
20090280544 | METHOD FOR PRODUCING OPTICALLY ACTIVE 2-BENZYLOXYCYCLOHEXYLAMINE - The present invention relates to a method for the enantioselective acylation of trans-2-benzyloxycyclohexylamine or cis-2-benzyloxycyclohexylamine, according to which an enantiomer mixture of 2-benzyloxycyclohexylamine is reacted with an acylation agent in the presence of a hydrolase. The invention also relates to a method for producing optically active trans-stereoisomers of 2-benzyloxycyclohexylamine. | 11-12-2009 |
20090325245 | Ethanolamine Production by Fermentation - The present invention provides a bacterium and a method for the biological production of ethanolamine from a fermentable carbon source. In one aspect of the present invention, a process for the conversion of glucose to ethanolamine is achieved by the use of a recombinant bacterium transformed i) to express a serine decarboxylase enzyme to convert serine to ethanolamine ii) to inactivate the ethanolamine consuming pathways and iii) to increase 3-phosphoglycerate availability. In another aspect of the present invention, the process for the production of ethanolamine from glucose using a recombinant | 12-31-2009 |
20100041110 | R-Hydroxynitrile Lyases Having Improved Substrate Acceptance and the Use Thereof - R-hydroxynitrile lyases having an improved substrate acceptance, increased activity and increased selectivity, in which there is replacement in the amino acid sequence of R-hydroxynitrile lyases from the Rosaceae family either a) of the amino acid residue which corresponds to position 360 of the mature PaHNL5 protein by another apolar amino acid or a neutral amino acid and/or b) of the amino acid residue which corresponds to position 225 of the mature PaHNL5 protein by another polar amino acid, it also being possible where appropriate for 1 to 20 further residues in the active center or in the hydrophobic channel leading to the active center to be replaced. | 02-18-2010 |
20100068771 | PROCESS FOR THE PREPARATION OF ENANTIOMERICALLY ENRICHED BETA-AMINO ALCOLHOLS STARTING FROM GLYCINE AND AN ALDEHYDE IN THE PRESENCE OF A THREONINE ALDOLASE AND A DECARBOXYLASE - The invention relates to a process for the preparation of an enantiomerically enriched β-amino alcohol, wherein glycine or a glycine salt and an aldehyde are reacted in the presence of a threonine aldolase and a decarboxylase to form the corresponding enantiomerically enriched β-aminoalcohol, and wherein at least either the threonine aldolase or the decarboxylase is β-selective. In a preferred embodiment of the invention at least either the threonine aldolase or the decarboxylase is enantioselective. | 03-18-2010 |
20100112648 | (R)-HYDROXYNITRILE LYASE FROM BRASSICACEAE - The invention concerns a polypeptide which can be isolated from the Brassicaceae family and which has at least the activity of a hydroxynitrile lyase (HNL). The hydroxynitrile lyase of the invention is the first HNL from the Brassicaceae family. The plants ( | 05-06-2010 |
20100143986 | R-HNL RANDOM VARIANTS AND THEIR USE FOR PREPARING OPTICALLY PURE, STERICALLY HINDERED CYANOHYDRINS - The invention relates to R-hydroxynitrile lyases with improved substrate acceptance, increased activity and increased selectivity, obtainable by introducing random mutations with the aid of random mutagenesis and/or saturation mutagenesis techniques, identifying by means of screening or selection and, where appropriate, subsequently combining advantageous mutations, and to the use thereof. | 06-10-2010 |
20100151534 | KETOREDUCTASE POLYPEPTIDES FOR THE PRODUCTION OF A 3-ARYL-3-HYDROXYPROPANAMINE FROM A 3-ARYL-3-KETOPROPANAMINE - The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N,N-dimethyl-3-keto-3-(2-thienyl)-1-ketopropanamine to (S)—N,N-dimethyl-3-hydroxy-3-(2-thienyl)-1-propanamine. | 06-17-2010 |
20100151535 | Renewable Chemical Production from Novel Fatty Acid Feedstocks - Disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Methods such as transesterification, hydrogenation, hydrocracking, deoxygenation, isomerization, interesterification, hydroxylation, hydrolysis and saponification are disclosed. Novel oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods of the invention. | 06-17-2010 |
20100151536 | Biological Synthesis of Difunctional Alkanes from Carbohydrate Feedstocks - Aspects of the invention relate to methods for the production of difunctional alkanes in host cells. In particular, aspects of the invention describe components of genes associated with the difunctional alkane production from carbohydrate feedstocks in host cells. More specifically, aspects of the invention describe metabolic pathways for the production of adipic acid, aminocaproic acid, caprolactam, and hexamethylenediamine via 2-ketopimelic acid. | 06-17-2010 |
20100151537 | MODIFIED ETHYLENEDIAMINE-N, N'-DISUCCINATE: ETHYLENEDIAMINE LYASE - The present invention provides a modified ethylenediamine-N,N′-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity. | 06-17-2010 |
20100173369 | KETOREDUCTASE POLYPEPTIDES FOR THE PRODUCTION OF 3-ARYL-3-HYDROXYPROPANAMINE FROM A 3-ARYL-3-KETOPROPANAMINE - The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine. | 07-08-2010 |
20100190218 | ENONE REDUCTASES - The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations. | 07-29-2010 |
20100203599 | MUTANT MICROORGANISMS HAVING A HIGH ABILITY TO PRODUCE PUTRESCINE AND METHOD FOR PRODUCING PUTRESCINE USING THE SAME - Provided are mutant microorganisms having the ability to produce a high concentration of putrescine wherein gene(s) involved in the putrescine degradation or utilization pathway is inactivated or deleted and a preparation method thereof. A method for producing putrescine in high yield by culturing the mutant microorganisms is also provided. The mutant microorganisms are useful for producing a high concentration of putrescine which can be widely used in various industrial applications. | 08-12-2010 |
20100203600 | METHOD FOR THE COPRODUCTION OF METHYL 7-OXOHEPTANOATE AND UNDECYLENIC ACID FROM RICINOLEIC ACID - The invention relates to a method for converting ricinoleic acid that comprises the following successive stages:
| 08-12-2010 |
20100209980 | METHOD FOR PRODUCING OPTICALLY ACTIVE AMINOALKYLPHENOLS - The invention relates to a method for the enantioselective N-acylation of aminoalkylphenols, and to a method for producing enantiomer-pure compounds of formulae (I-S) and/or (I-R). | 08-19-2010 |
20100209981 | TRANSAMINASE POLYPEPTIDES - The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds. | 08-19-2010 |
20100240105 | Method For Producing Glucosamine By Culturing Microorganism With Low-Cost Medium - A method for producing glucosamine with microorganism comprises of fermenting with a microorganism selected from the group consisting of | 09-23-2010 |
20100248317 | PROCESS FOR THE ENANTIOSELECTIVE ENZYMATIC REDUCTION OF INTERMEDIATES - A process for the enantioselective enzymatic reduction of a keto compound of general formula I | 09-30-2010 |
20100285544 | Novel aminotransferase, gene encoding the same, and method of using them - The invention relates to a novel aminotransferase, DNA encoding the enzyme, a recombinant vector into which the DNA has been introduced, and a transformant into which the vector has been introduced. Further, the invention also relates to a method for producing an optically active amino compound utilizing the enzyme or transformant. The aminotransferase of the invention has an ability of efficiently converting a ketone compound, particularly a cyclic ketone compound to an optically active amino compound. According to the invention, a method for efficiently producing an optically active amino compound, particularly an optically active cyclic amino compound is provided. | 11-11-2010 |
20100291640 | PROCESS FOR PREPARING URETHANE-CONTAINING (METH)ACRYLIC ESTERS - A process for preparing urethane-containing (meth)acrylic esters (U) by reacting a urethane-containing alcohol (A) with a (meth)acrylic ester of a saturated alcohol (G) in the presence of at least one polymerization inhibitor (P) with an enzyme (E) as a catalyst in a reactor, wherein
| 11-18-2010 |
20100291641 | PROCESS FOR CONTINUOUSLY PREPARING URETHANE-CONTAINING (METH)ACRYLIC ESTERS - A process for preparing urethane-containing (meth)acrylic esters (U) by reacting a urethane-containing alcohol (A) with a (meth)acrylic ester of a saturated alcohol (G) in the presence of at least one polymerization inhibitor (P) with an enzyme (E) as a catalyst in a reactor, wherein the (meth)acrylic ester of a saturated alcohol (G) and the urethane-containing alcohol (A) are passed continuously through at least one fixed bed reactor filled with an immobilized enzyme (E) as a catalyst. | 11-18-2010 |
20100291642 | Process For Preparing Dipeptidyl Peptidase IV Inhibitors And Intermediates Therefor - A process for production of cyclopropyl-fused pyrrolidine-based inhibitors of dipeptidyl peptidase IV is provided which employs a BOC-protected amine of the structure | 11-18-2010 |
20110008852 | CONVERGENT SYNTHESIS OF RENIN INHIBITORS AND INTERMEDIATES USEFUL THEREIN - Described is a method for the preparation of renin inhibitors such as aliskiren, and intermediates useful therein. The method introduces a nitrogen-containing intermediate such as a lactone of formula (8). with R | 01-13-2011 |
20110039313 | METHOD FOR THE FERMENTATIVE PRODUCTION OF CADAVERINE - The invention relates to recombinant microorganisms in which polynucleotides which code for lysine decarboxylase are enhanced and, using which, cadaverine (1,5-diaminopentane) is produced fermentatively, with the carbon source used preferably being renewable raw materials such as, for example, glucose, sucrose, molasses and the like. | 02-17-2011 |
20110045553 | PROCESS FOR CONVERTING TRYPTOPHAN INTO KYNURENINE - A process for converting tryptophan into kynurenine. The conversion of tryptophan into kynurenine is carried out by irradiating a substance comprising tryptophan with electromagnetic radiation comprising waves with wavelength in the range of 10 nanometer to 750 nanometer. | 02-24-2011 |
20110065154 | Deracemisation of Amines - The present invention relates to a method for the deracemisation or chiral inversion of chiral amines by enzymatic treatment. The method employs a stereoselective enzymatic conversion and either a non-selective or partially selective chemical or enzymatic conversion, simultaneously or sequentially. The invention also provides a method for selecting a suitable enzyme, particularly a suitable amine oxidase, and for the generation of novel enzymes suitable for use in the deracemisation method. | 03-17-2011 |
20110097767 | MICROORGANISMS FOR THE PRODUCTION OF ANILINE - A non-naturally occurring microbial organism having an aniline pathway includes at least one exogenous nucleic acid encoding an aniline pathway enzyme expressed in a sufficient amount to produce aniline. The aniline pathway includes (1) an aminodeoxychorismate synthase, an aminodeoxychorismate lyase, and a 4-aminobenzoate carboxylyase or (2) an anthranilate synthase and an anthranilate decarboxylase. A method for producing aniline, includes culturing these non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce aniline. | 04-28-2011 |
20110104764 | METHOD FOR SYNTHESISING OMEGA-AMINO-ALKANOIC ACIDS OR THE ESTERS THEREOF FROM NATURAL FATTY ACIDS - The invention relates to a method for synthesizing ω-amino-alkanoic acids or the esters thereof from mono-unsaturated natural fatty acids comprising at least one step of forming the unsaturated diacid corresponding to the original fatty acid. | 05-05-2011 |
20110111469 | PROCESS FOR THE PRODUCTION OF N-ACYL-PHOSPHATIDYL-ETHANOLAMINE - The document describes a process for the preparation of N-Acyl-Phosphatidyl-Ethanolamine of formula (I) on an industrial scale, In which R | 05-12-2011 |
20110151523 | Nitrogen Compound Production Method and System - A nitrogen compound production method includes the step of establishing at least one aquatic plant in a cell containing water and alternating a step of inhibiting photosynthesis inducing light from reaching the at least one aquatic plant and a step of allowing photosynthesis inducing light to reach the at least one aquatic plant. The step of inhibiting photosynthesis inducing light defines a dark phase. A concentration of oxygen is reduced in the water to define an anoxic condition. During the anoxic condition, the at least one plant releases nitrogen compounds into the water and these nitrogen compounds are collected from the water contained in the cell. | 06-23-2011 |
20110165636 | PROCESS FOR THE STEREOSELECTIVE ENZYMATIC HYDROLYSIS OF 5-METHYL-3-NITROMETHYL-HEXANOIC ACID ESTER - The present invention relates to processes for the preparation of 5-methyl-3-nitromethyl-hexanoic acid ester and its salts. Also disclosed are processes for the preparation of 5-methyl-3-nitromethyl-hexanoic acid salt and a process for the preparation of 3-(aminomethyl)-5-methylhexanoic acid. (S)-5-Methyl-3-nitromethyl-hexanoic acid or (R)-5-methyl-3-nitromethyl-hexanoic acid in enantioenriched form or enantiopure form as well as salts thereof, (S)-5-methyl-3-nitromethyl-hexanoic acid ester or (R)-5-methyl-3-nitromethyl-hexanoic acid ester in enantioenriched form or enantiopure form and a compound, namely Formula (XIII), in racemic form, enantioenriched form or enantiopure form are also disclosed. | 07-07-2011 |
20110171699 | PREPARATION OF 6-AMINOCAPROIC ACID FROM 5 -FORMYL VALERI C ACID - The invention relates to a method for preparing 6-aminocaproic acid (hereinafter also referred to as ‘6-ACA’) using a biocatalyst. The invention further relates to a method for preparing e-caprolactam (hereafter referred to as ‘caprolactam’) by cyclising such 6-ACA. The invention further relates to a host cell, a micro-organism, or a polynucleotide which may be used in the preparation of 6-ACA or caprolactam. | 07-14-2011 |
20110171700 | METHOD FOR PRODUCING L-PHENYLEPHRINE USING AN ALCOHOL DEHYDROGENASE OF AROMATOLEUM AROMATICUM EBN1 (AZOARCUS SP. EBN1) - The present invention relates to a multi-stage process for producing substituted, optically active alcohols, comprising an enzyme-catalyzed synthesis step, in particular a synthesis step which is catalyzed by an alcohol dehydrogenase. The inventive method is particularly suitable for producing phenylephrine, i.e. 3-[(1R)-1-hydroxy-2-methylamino-ethyl]-phenol. | 07-14-2011 |
20110250657 | Processes for Making [R]-Ethyl 4-Cyano-3 Hydroxybutyric Acid - The invention provides novel processes for making ethyl-4-cyano-3-hydroxybutyrate, e.g., (R)-ethyl 4-cyano-3-hydroxybutyric acid, and 4-cyano-3-hydroxybutyric acid. The invention provides protocols for making and 4-cyano-3-hydroxybutyric acid and ethyl-4-cyano-3-hydroxybutyrate by whole cell processes, cell lysate processes, “one pot processes” and “multi-pot” processes using a variety of parameters. | 10-13-2011 |
20110300590 | Method for Synthesizing an Omega-Amino Acid or Ester from a Monounsaturated Fatty Acid or Ester - The invention relates to a method for synthesizing ω-aminoalkanoic acids or esters thereof from unsaturated natural fatty acids, passing through a monounsaturated dinitrile intermediate compound. The method of the invention is simple to carry out and, compared to other known methods, avoids the environmental constraints and economic disadvantages due to reaction by-products. | 12-08-2011 |
20110300591 | Bacterial Compositions of Staphylococcus Vitulinus Having Nitrate Reductase Activity and of Lactic Acid Bacteria and Methods Using These Compositions - The invention relates to a method for converting nitrates to nitrites and to specific compositions of bacteria belonging to the | 12-08-2011 |
20110318793 | Method of Producing a Low Molecular Weight Organic Compound in a Cell - A method of producing a low molecular weight organic compound (e.g. a plant or bacteria secondary metabolite) in increased yields involving use of a microorganism cell, which comprises a gene involved in the biosynthesis pathway leading to a low molecular weight organic aglycon compound and a glycosyltransferase gene capable of glycosylating the produced aglycon. | 12-29-2011 |
20120015412 | Preparation of Pregabalin and Related Compounds - Materials and methods for preparing (S)-(+)-3-aminomethyl-5-methyl-hexanoic acid and structurally related compounds via enzymatic kinetic resolution are disclosed. | 01-19-2012 |
20120028317 | HYDROXYNITRILE LYASE - An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture. | 02-02-2012 |
20120040417 | HYDROXYNITRILE LYASE - An improved hydroxynitrile lyase characterized by having a mutation of substitution of at least one amino acid residue in the amino acid sequence of a wild-type hydroxynitrile lyase with another amino acid and by its hydroxynitrile lyase activity per transformant being higher than the hydroxynitrile lyase activity per transformant into which the wild-type hydroxynitrile lyase gene is introduced; and a method for producing a hydroxynitrile lyase, comprising expressing the improved hydroxynitrile lyase in a host and recovering the improved hydroxynitrile lyase from the resultant culture. | 02-16-2012 |
20120100580 | METHOD FOR PRODUCING PHOSPHOLIPID - Provided is a method for producing a phospholipid at low cost by reusing phospholipase A2 in a method for producing the phospholipid whereby an arbitrary fatty acid is bonded to the 2-position of a phospholipid using an esterification reaction catalyzed by phospholipase A2 in glycerol. The method for producing a phospholipid is characterized by comprising conducting an esterification reaction catalyzed by phospholipase A2 between a lysophospholipid and an acyl donor in glycerol to from a phospholipid, adding a solvent immiscible with glycerol to form a glycerol layer and a solvent layer, extracting said phospholipid into said solvent layer, allowing phospholipase A2 to migrate into said glycerol layer, and, after separating the glycerol layer and distilling off the solvent remaining therein, further adding to the residual glycerol solution the lysophospholipid and the acyl donor to thereby conduct the esterification reaction again with use of phospholipase A2 remaining in said glycerol solution. | 04-26-2012 |
20120149073 | Ketoreductase Polypeptides for the Preparation of Phenylephrine - The disclosure relates to engineered ketoreductase polypeptides and processes of using the polypeptides for production of phenylephrine. | 06-14-2012 |
20120190087 | PREPARATION OF 1,4-DIAMINOBUTANE - The present invention relates to a novel method for the preparation of 1,4-diaminobutane [DAB]. The method according to the present invention involves at least one biocatalytic step which comprises the biocatalytic production of at least one N-protected precursor of DAB. The present invention also relates to a method for the preparation of DAB involving at least one biocatalytic step, and comprising the steps of a) biocatalytically preparing an N-protected precursor of DAB yielding a—biocatalytic reaction mixture containing the N-protected precursor of DAB, b) recovering the N-protected precursor from the biocatalytic reaction mixture and c) converting the N-protected precursor into DAB. More in particular, the present invention relates to a method for the preparation of DAB, wherein the at least N-protected precursor of DAB is selected from the group consisting of N5-protected ornithine, N-protected DAB, and N-protected 4-aminobutyraldehyde. | 07-26-2012 |
20120196334 | LIGF-TYPE ENZYMES FOR BIOCONVERSION OF LIGNIN-DERIVED COMPOUNDS - The teachings provided herein are generally directed to a method of converting lignin-derived compounds to valuable aromatic chemicals using an enzymatic, bioconversion process. The teachings provide a selection of (i) host cells that are tolerant to the toxic compounds present in lignin fractions; (ii) polypeptides that can be used as enzymes in the bioconversion of the lignin fractions to the aromatic chemical products; (iii) polynucleotides that can be used to transform the host cells to express the selection of polypeptides as enzymes in the bioconversion of the lignin fractions; and (iv) the transformants that express the enzymes. | 08-02-2012 |
20120196335 | LIGF-TYPE TRANSFORMANTS FOR BIOCONVERSION OF LIGNIN-DERIVED COMPOUNDS - The teachings provided herein are generally directed to a method of converting lignin-derived compounds to valuable aromatic chemicals using an enzymatic, bioconversion process. The teachings provide a selection of (i) host cells that are tolerant to the toxic compounds present in lignin fractions; (ii) polypeptides that can be used as enzymes in the bioconversion of the lignin fractions to the aromatic chemical products; (iii) polynucleotides that can be used to transform the host cells to express the selection of polypeptides as enzymes in the bioconversion of the lignin fractions; and (iv) the transformants that express the enzymes. | 08-02-2012 |
20120202257 | LIGE-TYPE ENZYMES FOR BIOCONVERSION OF LIGNIN-DERIVED COMPOUNDS - The teachings provided herein are generally directed to a method of converting lignin-derived compounds to valuable aromatic chemicals using an enzymatic, bioconversion process. The teachings provide a selection of (i) host cells that are tolerant to the toxic compounds present in lignin fractions; (ii) polypeptides that can be used as enzymes in the bioconversion of the lignin fractions to the aromatic chemical products; (iii) polynucleotides that can be used to transform the host cells to express the selection of polypeptides as enzymes in the bioconversion of the lignin fractions; and (iv) the transformants that express the enzymes. | 08-09-2012 |
20120202258 | LIGE-TYPE TRANSFORMANTS FOR BIOCONVERSION OF LIGNIN-DERIVED COMPOUNDS - The teachings provided herein are generally directed to a method of converting lignin-derived compounds to valuable aromatic chemicals using an enzymatic, bioconversion process. The teachings provide a selection of (i) host cells that are tolerant to the toxic compounds present in lignin fractions; (ii) polypeptides that can be used as enzymes in the bioconversion of the lignin fractions to the aromatic chemical products; (iii) polynucleotides that can be used to transform the host cells to express the selection of polypeptides as enzymes in the bioconversion of the lignin fractions; and (iv) the transformants that express the enzymes. | 08-09-2012 |
20120244583 | Method for Preparing High Purity L-alpha Glycerylphosphorylcholine - Disclosed is a method for preparing L-α-Glycerylphosphorylcholine with high yields and purity. The method uses phospholipase A | 09-27-2012 |
20120258504 | BIOLOGICAL SYNTHESIS OF DIFUNCTIONAL ALKANES FROM CARBOHYDRATE FEEDSTOCKS - Aspects of the invention relate to methods for the production of difunctional alkanes in host cells. In particular, aspects of the invention describe components of genes associated with the difunctional alkane production from carbohydrate feedstocks in host cells. More specifically, aspects of the invention describe metabolic pathways for the production of adipic acid, aminocaproic acid, caprolactam, and hexamethylenediamine via 2-ketopimelic acid. | 10-11-2012 |
20120264180 | PROCESS FOR THE BIOLOGIC TREATMENT OF ORGANIC WASTES AND PLANT THEREFOR - Process for the biological treatment of organic wastes, includes a first anaerobic digestion phase and a second aerobic digestion phase in succession, a respective filtration phase of the solid substance being provided between the two digestion phases, a separation phase of the biogas released in the anaerobic digestion phase and a nitrogen recovery phase from the exhaust gases rich in ammoniac substances separated from the aerobic digestion phase. The solid substance coming from each of the filtration phases is separately returned to the respective digestion phase, while the liquid phase coming from the filtration phase downstream of the anaerobic digestion phase is sent to aerobic phase. In the nitrogen recovery phase the gaseous current consisting of exhaust gases rich in ammoniac substances is treated with a solution of carbonic acid supplied in countercurrent in a first gas/liquid contactor to obtain a mixture of nitrogen salts (ammonium bicarbonate, ammonium carbonate, etc.). | 10-18-2012 |
20120276600 | Process For Preparing Dipeptidyl Peptidase IV Inhibitors And Intermediates Therefor - A process for production of cyclopropyl-fused pyrrolidine-based inhibitors of dipeptidyl peptidase IV is provided which employs a BOC-protected amine of the structure | 11-01-2012 |
20120295317 | Processes and Recombinant Microorganisms for the Production of Cadaverine - The present invention relates to the use of recombinant microorganisms comprising DNA molecules in a deregulated form which improve the production of cadaverine, as well as to recombinant DNA molecules and polypeptides used to produce the microorganism, said microorganism comprising an intracellular lysine decarboxylase activity and an enhanced lysine import activity or comprising an intracellular and an extracellular lysine decarboxylase activity or comprising an intracellular and extracellular lysine decarboxylase activity and an enhanced lysine import activity. The present invention also relates to a processes for the production of cadaverine using recombinant microorganisms. | 11-22-2012 |
20120309062 | MICROORGANISMS FOR THE PRODUCTION OF ADIPIC ACID AND OTHER COMPOUNDS - The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam. | 12-06-2012 |
20130005002 | AMIDASE AND USE THEREOF FOR PRODUCING 3-AMINOCARBOXYLIC ACID ESTERS - Process for producing optically active 3-aminocarboxylic acid ester compounds of general Formula I, and the ammonium salts thereof, | 01-03-2013 |
20130034885 | PROCESS FOR THE ENZYMATIC PRODUCTION OF CARNITINE FROM BETA-LACTONES - Subject of the invention is a process for the production of L-carnitine, wherein a β-lactone, which is a 4-(halomethyl)oxetane-2-one, is converted into L-carnitine, wherein the process comprises an enzymatic conversion of the β-lactone into (R)-4-halo-3-hydroxybutyric acid or (R)-4-halo-3-hydroxybutyric acid ester. | 02-07-2013 |
20130071888 | METHOD FOR PRODUCING 1,5-PENTANEDIAMINE - A method of producing 1,5-pentanediamine includes culturing coryneform bacterium having a gene encoding lysine decarboxylase in its chromosome, which coryneform bacterium maintains lysine decarboxylase activity of not less than 50 mU/mg protein during culturing and the gene encoding lysine decarboxylase is linked downstream of a promoter that functions during the logarithmic growth phase. | 03-21-2013 |
20130089898 | BIOCATALYSTS AND METHODS FOR THE SYNTHESIS OF (S)-3-(1-AMINOETHYL)-PHENOL - The present disclosure provides engineered transaminase polypeptides having improved properties as compared to naturally occurring transaminases including the ability of converting the substrate, 3′-hydroxyacetophenone to (S)-3-(1-aminoethyl)-phenol in enantiomeric excess and high percentage conversion. Also provided are polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to synthesize (S)-3-(1-aminoethyl)-phenol and related compounds useful in the production of active pharmaceutical ingredients. | 04-11-2013 |
20130095534 | PROCESS FOR PRODUCTION OF CADAVERINE - By a method for producing cadaverine by culturing a microorganism that extracellularly secretes lysine decarboxylase, by-production of lysine is suppressed, the yield of cadaverine relative to glucose consumption is improved compared to conventional production methods, and further, the load on the purification step in purification of cadaverine as a raw material for polyamide can be reduced. | 04-18-2013 |
20130115663 | ENONE REDUCTASES - The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations. | 05-09-2013 |
20130149752 | (R)-HYDROXYNITRILE LYASE FROM BRASSICACEAE - The invention concerns a polypeptide which can be isolated from the Brassicaceae family and which has at least the activity of a hydroxynitrile lyase (HNL). The hydroxynitrile lyase of the invention is the first HNL from the Brassicaceae family. The plants ( | 06-13-2013 |
20130157323 | MUTANT MICROORGANISM HAVING HIGH PRODUCTION OF CADAVERINE, AND PREPARATION METHOD OF CADAVERINE USING SAME - The present invention relates to a mutant microorganism having a high ability to produce cadaverine, and a method for producing cadaverine using the same. More specifically, the invention relates to a mutant microorganism having a high ability to produce cadaverine wherein a gene involved in the cadaverine degradation or utilization pathway is inactivated or deleted, and to a method for producing cadaverine in high yield by culturing the mutant microorganism under aerobic conditions. The mutant microorganism according to the present invention is useful for producing a high yield of cadaverine which can be widely used in various industrial applications. | 06-20-2013 |
20130164794 | BIOCATALYSTS AND METHODS FOR THE SYNTHESIS OF (1R,2R)-2-(3,4-DIMETHOXYPHENETHOXY)CYCLOHEXAMINE - The disclosure provides transaminase polypeptides capable of converting the substrate, 2-(3,4-dimethoxyphenethoxy)cyclohexanone to the trans diastereomer product (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine in at least a 2:1 diastereomeric ratio relative to the cis diastereomer (1R,2S)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine. The disclosure also provides polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides in processes for preparing (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine and its analogs, which can product compounds can be further used to prepare the aminocyclohexylether compound, (3R)-1-[(1R,2R)-2-[2-(3,4-dimethoxyphenyl)ethoxy]cyclohexyl]pyrrolidin-3-ol, which is an ion channel blocker. | 06-27-2013 |
20130177951 | CHEMO-ENZYMATIC PROCESS FOR PREPARING QUATERNARY AMMONIUM ESTERS - A process for producing surface-active quaternary ammonium esters is provided. These esters are advantageously prepared in high yield and purity by a chemo-enzymatic process. These compounds have excellent surfactant properties. | 07-11-2013 |
20130196389 | NOVEL TRANSAMINASE EXHIBITING HIGH ACTIVITY FOR GLUTAMIC ACID, GENE ENCODING SAME, AND METHOD FOR USING THEM - A method for inexpensively and efficiently producing an optically active amino compound useful as an intermediate for pharmaceutical preparations, agricultural chemicals, or the like, from a ketone compound is provided. Specifically, a polypeptide exhibiting higher activity for glutamic acid as an amino donor than that for L-alanine, and, having novel transaminase activity for generating (S)-1-benzyl-3-pyrrolidinone with high optical purity of 93% or more, a gene encoding the same, and a transformant expressing the gene at a high level are also provided herein. | 08-01-2013 |
20130244298 | METHOD FOR BIOCATALYTIC PRODUCTION OF NITRILES FROM OXIMES AND OXIME DEHYDRATASES USABLE THEREIN - The present invention relates to novel methods for biocatalytic production of nitriles from oximes using oxime dehydratases and novel mutants with oxime dehydratase activity and use thereof in a process for biocatalytic production of nitriles, such as in particular for the production of citral nitrile, neral nitrile, geranial nitrile or citronellyl nitrile from citral oxime, neral oxime, geranial oxime or citronellal oxime; and oxime dehydratases usable therefor, nucleotide sequences therefor and expression constructs or microorganisms comprising these. | 09-19-2013 |
20130266994 | TRANSAMINASE POLYPEPTIDES - The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds. | 10-10-2013 |
20130288315 | Nitrilases - The invention relates to a nitrilase having improved activity in the reaction of a nitrile to form the corresponding carboxylic acid, in particular with respect to reacting 2-methylglutaronitrile, 1-(cyanomethyl)cyclohexane-1-carbonitrile, and benzonitrile. The nitrilase according to the invention is related to nitrilase from | 10-31-2013 |
20130302860 | Coryneform Bacterium Transformant and Process for Producing Aniline Using The Same - Provided is an aniline-producing transformant constructed by introducing a gene which encodes an enzyme having aminobenzoate decarboxylase activity into a coryneform bacterium as a host. Also provided is a process for producing aniline, which comprises a step of allowing the transformant to react in a reaction mixture containing aminobenzoic acid, an ester thereof, and/or a salt thereof under reducing conditions, and a step of recovering aniline from the reaction mixture. | 11-14-2013 |
20130309732 | BIOSYNTHESIS METHODS OF NOREPHEDRINE WITH SPECIFIC OPTICAL ACTIVITIES - A biosynthesis method of norephedrine with specific optical activities is revealed to convert and generate optical isomers with specific optical activities by biocatalysis. A two-step biotransformation reaction is carried by a whole-cell biocatalyst for converting reaction substrates, benzaldehyde and pyruvate, to L-phenylacetylcarbinol (L-PAC) in the first step and the yield of the L-PAC is 99%, and then an amino donor (L-alanine) is added and the transamination of the L-PAC is catalyzed by a transaminase with optical specificity for biosynthesizing the norephedrine with high optical purity. The pyruvate is produced from the amino donor, L-alanine, by the transamination in the reaction system, so that the pyruvate is regenerated in the reaction system without being added again. | 11-21-2013 |
20130309733 | STABILIZED RECOMBINANT EXPRESSION PLASMID VECTOR IN HAFNIA ALVEI AND APPLICATIONS THEREOF - One aspect of the present disclosure relates to a stabilized recombinant expression plasmid vector comprising a polynucleotide encoding an antitoxin gene which expresses a polypeptide that neutralizes a polypeptide toxic to a host cell, the toxic polypeptide being expressed by a toxin gene in the host cell, and a polynucleotide encoding a polypeptide expression product, and the stabilized recombinant expression plasmid vector is derived from a | 11-21-2013 |
20130309734 | Engineered Amine Dehydrogenases and Methods of Use Thereof - Non-naturally occurring amine dehydrogenases (AmDH) and methods of use thereof the produce chiral amines are disclosed. The AmDH are variants of amino acid dehydrogenases. AmDH based on phenylalanine, leucine, and valine scaffolds are provided. The AmDH typically have one, two, three, four, or more amino acid alterations relative to the scaffold. The alterations to the scaffold result in an enzyme that accepts the analogous ketone, such as methyl isobutyl ketone (MIBK), instead of the wild-type α-keto acid. Chimeric AmDH are also disclosed. The chimeras are fusion proteins that include a substrate binding domain from a first AmDH and a cofactor binding domain from a second AmDH. In a preferred embodiment, one of the domains is from a PheDH-based AmDH and one of the domains is from a LeuDH-based AmDH. | 11-21-2013 |
20130316409 | Nitrilases, Nucleic Acids Encoding Them and Methods for Making and Using Them - The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature. | 11-28-2013 |
20130323800 | METHOD FOR PRODUCING CADAVERINE - A method produces cadaverine more efficiently and at a higher yield than production methods by the conventional fermentation methods. The method includes culturing coryneform bacterium/bacteria having an ability to produce cadaverine and having a resistance to 2,2′-thiobis(ethylamine). Preferably, the coryneform bacterium/bacteria has/have lysine decarboxylase activity and, preferably, the coryneform bacterium/bacteria has/have homoserine auxotrophy and/or a resistance to S-(2-aminoethyl)-L-cysteine. | 12-05-2013 |
20130337512 | Amidase and Use Thereof for Producing 3-Aminocarboxylic Acid Esters - Process for producing optically active 3-aminocarboxylic acid ester compounds of general Formula I, and the ammonium salts thereof, | 12-19-2013 |
20140004576 | METHOD FOR PRODUCING CADAVERINE | 01-02-2014 |
20140004577 | MICROORGANISMS FOR PRODUCING PUTRESCINE AND METHOD FOR PRODUCING PUTRESCINE USING SAME | 01-02-2014 |
20140080187 | SERINOL PRODUCTION IN GLYCEROL CATABOLISM DEFICIENT ESCHERICHIA COLI STRAINS - The invention is about | 03-20-2014 |
20140099682 | Enzymatic Synthesis of Optically Active Chiral Amines - The present invention relates to method of production of optically active chiral amine from alpha hydroxy ketone using enzyme transaminase as the biocatalyst. In particular the present invention relates to production of (1R, 2S)-Norephedrine and its salts from R-Phenylacetylcarbinol (R-PAC) by employing S-transaminase as the biocatalyst and Isopropylamine as the amine donor. | 04-10-2014 |
20140134681 | PREPARATION OF 6-AMINOCAPROIC ACID FROM 5-FORMYL VALERI C ACID - The invention relates to a method for preparing 6-aminocaproic acid (hereinafter also referred to as ‘6-ACA’) using a biocatalyst. The invention further relates to a method for preparing e-caprolactam (hereafter referred to as ‘caprolactam’) by cyclising such 6-ACA. The invention further relates to a host cell, a micro-organism, or a polynucleotide which may be used in the preparation of 6-ACA or caprolactam. | 05-15-2014 |
20140154754 | SYSTEMS AND METHODS FOR ANAEROBIC DIGESTION AND COLLECTION OF PRODUCTS - Described herein are methods and systems that can provide independent energy generation as well as systems for the generation of other useful products, such as chemicals. In addition, the systems and methods can provide ways of harnessing the potential of biomass feedstock to generate of multitude of products including green crude, biogas, electricity, heat, fatty acids, biodiesel, ammonia, and chemical products. In many cases, the systems and methods herein utilize anaerobic microorganisms, including aquatic and ruminant organisms, to digest material and create products. | 06-05-2014 |
20140170715 | Polypeptides Having Peroxygenase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 06-19-2014 |
20140178948 | PRODUCTION OF OMEGA-AMINO FATTY ACIDS - The invention provides a whole cell catalyst which expresses a recombinant α-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the α-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a α-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase. | 06-26-2014 |
20140186904 | Methods Of Producing 7-Carbon Chemicals Via Methyl-Ester Shielded Carbon Chain Elongation - This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on enzymes or homologs accepting methyl ester shielded dicarboxylic acid substrates. | 07-03-2014 |
20140186905 | BIOTECHNOLOGICAL SYNTHESIS PROCESS OF ORGANIC COMPOUNDS WITH THE AID OF AN ALKL GENE PRODUCT - Subject matter of the invention is a biotechnological process for the production of organic compounds with the aid of at least one alkL gene product. | 07-03-2014 |
20140193861 | Methods Of Producing 7-Carbon Chemicals Via Aromatic Compounds - This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate produced from chorismate or benzoate. These pathways, metabolic engineering and cultivation strategies described herein rely on the anaerobic benzoyl-CoA degradation pathway enzymes. | 07-10-2014 |
20140193862 | Methods Of Producing 7-Carbon Chemicals Via Carbon Chain Elongation Associated With Cyclohexane Carboxylate Synthesis - This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the carbon chain elongation enzymes or homologs thereof associated with the cyclohexane carboxylate biosynthesis from | 07-10-2014 |
20140193863 | Methods Of Producing 7-Carbon Chemicals Via C1 Carbon Chain Elongation Associated With Coenzyme B Synthesis - This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming one or two terminal functional groups, each comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the C1 elongation enzymes or homolog associated with coenzyme B biosynthesis. | 07-10-2014 |
20140193864 | Methods Of Producing 7-Carbon Chemicals Via Pyruvate And Succinate Semialdehyde Aldol Condensation - This document describes biochemical pathways for producing one or more of pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine and 1,7-heptanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C7 aliphatic backbone substrate produced from succinate semialdehyde or pyruvate. These pathways, metabolic engineering and cultivation strategies described herein rely on the aldol condensation of succinate semialdehyde and pyruvate. | 07-10-2014 |
20140193865 | Methods Of Producing 7-Carbon Chemicals From Long Chain Fatty Acids Via Oxidative Cleavage - This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the fatty acid synthesis pathway and oxidative cleavage of long chain acyl-[acp] intermediates by a monooxgenase (e.g., cytochrome P450) such as that encoded by BioI from microorganisms such as | 07-10-2014 |
20140199736 | PICHIA CIFERRII CELLS AND USE THEREOF - The invention relates to genetically modified | 07-17-2014 |
20140212933 | OVER-EXPRESSION OF A PUTATIVE OXIDOREDUCTASE (UCPA) FOR INCREASING FURFURAL OR 5-HYDROXYMETHYLFURFURAL TOLERANCE - The subject invention pertains to overexpression of a putative oxidoreductase (ucpA) for increasing furfural tolerance in genetically modified microorganisms. Genetically modified microorganisms capable of overexpressing UcpA are also provided. Increased expression of ucpA was shown to increase furfural tolerance by 50%, and to permit the fermentation of sugars to products in the presence of 15 mM furfural. | 07-31-2014 |
20140212934 | BIOLOGICAL SYNTHESIS OF DIFUNCTIONAL HEXANES AND PENTANES FROM CARBOHYDRATE FEEDSTOCKS - Provided herein are methods for the production of difunctional alkanes in microorganisms. Also provided are enzymes and nucleic acids encoding such enzymes, associated with the difunctional alkane production from carbohydrates feedstocks in microorganisms. The invention also provides recombinant microorganisms and metabolic pathways for the production of difunctional alkanes. | 07-31-2014 |
20140242646 | OXIDATION AND AMINATION OF PRIMARY ALCOHOLS - The present invention relates to a method comprising the steps a) providing a primary alcohol of the formula HO—(CH | 08-28-2014 |
20140248673 | Methods of Producing 7-Carbon Chemicals via CoA-Dependent Carbon Chain Elongation Associated with Carbon Storage - This document describes biochemical pathways for producing pimelic acid, 7-hydroxyheptanoic acid, 7-aminoheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the CoA-dependent elongation enzymes or analog enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria. | 09-04-2014 |
20140295506 | METHOD TO PRODUCE HYDRAZIDE - A method to produce hydrazide from triacylglycerol, the method includes steps of mixing vegetable oil with an organic solvent in a reactor forming a mixture, adding hydrazinemonohydrate into the mixture, stirring the mixture, adding catalyst, stirring the mixture to form hydrazide and separating the hydrazide from the mixture. Most illustrative drawing: | 10-02-2014 |
20140363859 | RECOMBINANT MICROORGANISM HAVING IMPROVED PUTRESCINE PRODUCING ABILITY AND METHOD FOR PRODUCING PUTRESCINE BY USING SAME - The present invention relates to a recombinant microorganism having enhanced ability to produce putrescine at high yield, wherein the activity of NCgl0101 is weakened in a microorganism of genus | 12-11-2014 |
20150031094 | POLYNUCLEOTIDES ENCODING ENGINEERED KETOREDUCTASE POLYPEPTIDES - The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine. | 01-29-2015 |
20150031095 | ENONE REDUCTASES - The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations. | 01-29-2015 |
20150050706 | DILUTE CHEMICAL REACTION PROCESS - Disclosed is a process for carrying out a cyclisation reaction, a polymerization reaction, an enzymatic reaction showing substrate inhibition, an enzymatic reaction showing product inhibition, a reaction showing precipitation of the substrate or of a reactant, the process comprising the steps of
| 02-19-2015 |
20150104838 | RECOMBINANT MICROORGANISM HAVING IMPROVED PUTRESCINE PRODUCING ABILITY AND METHOD FOR PRODUCING PUTRESCINE BY USING SAME - The present invention relates to a recombinant microorganism having enhanced ability of producing putrescine at high yield, which is generated by weakening the activity of NCgl1469 in a microorganism of | 04-16-2015 |
20150111262 | METHODS AND MATERIALS FOR PRODUCING FIVE CARBON BUILDING BLOCKS FROM PROLINE - This document describes biochemical pathways for producing glutaric acid, 5-aminopentanoic acid, 5-hydroxypentanoic acid, cadaverine or 1,5-pentanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C5 backbone substrate such as D-proline. | 04-23-2015 |
20150132808 | METHOD FOR PRODUCING 1,5-PENTAMETHYLENEDIAMINE, MUTANT LYSINE DECARBOXYLASE, METHOD FOR PRODUCING 1,5-PENTAMETHYLENE DIISOCYANATE AND METHOD FOR PRODUCING POLYISOCYANATE COMPOSITION - A mutant lysine decarboxylase is produced by replacing at least one of the amino-acids in the amino acid sequence shown in sequence ID No. 4 of the sequence listing with another amino-acid. | 05-14-2015 |
20150140617 | CANDIDA TROPICALIS CELLS AND USE THEREOF - The invention relates to genetically engineered | 05-21-2015 |
20150291987 | PATHWAYS TO ADIPATE SEMIALDEHYDE AND OTHER ORGANIC PRODUCTS - Recombinant microorganisms comprising at least one exogenous nucleic acid sequence and capable of producing adipate semialdehyde are provided. Adipate semialdehyde may be produced in a synthesis pathway utilizing a single thiolase reaction. Adipate semialdehyde may also be produced from intermediates consisting of alpha, omega difunctional aliphatic organic molecules. Methods of using recombinant microorganisms to produce 6-aminocaproic acid, adipic acid, hexamethylenediamine and 1.6-hexanediol are also provided. | 10-15-2015 |
20150299635 | METHOD FOR ORGANIC WASTE HYDROLYSIS AND ACIDIFICATION AND AN APPARATUS THEREOF - The invention relates to a method and an apparatus for hydrolyzing and acidifying homogenized organic waste fed into a closed and gas tight reactor using enzymatic bacteria under thermophilic conditions where water may be added to said reactor and the content of the reactor can be mixed and subjecting during hydrolysis and acidification an adequate part of a suspension formed in the reactor to—i. a separation step isolating—a permeate comprising an aqueous solution of soluble carbon, volatile fatty acids (VFA) and valuable nutrients, —an organic slurry comprising an aqueous solution of insoluble organic solids rich in enzymatic thermophilic bacteria and non-hydrolyzed organic solids, and the remaining part of soluble carbon, VFA and valuable nutrients, thereafter to—ii. a recirculation step re-feeding said organic slurry into the reactor for further hydrolysis and acidification. | 10-22-2015 |
20150307906 | PRODUCING AMINES AND DIAMINES FROM A CARBOXYLIC ACID OR DICARBOXYLIC ACID OR A MONOESTER THEREOF - The invention relates to a whole-cell catalyst which expresses a recombinant α-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase which phosphopantetheinylates the fatty acid reductase, and which expresses, in addition to the α-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase, a transaminase, wherein the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and also to a process for converting a carboxylic acid or dicarboxylic acid or a monoester thereof to an amine or diamine, comprising the steps of contacting the carboxylic acid or dicarboxylic acid or the monoester thereof with a phosphopantetheinylated fatty acid reductase or an α-dioxygenase and contacting the product with a transaminase. | 10-29-2015 |
20150322441 | BIOLOGICAL SYNTHESIS OF DIFUNCTIONAL ALKANES FROM CARBOHYDRATE FEEDSTOCKS - Aspects of the invention relate to methods for the production of difunctional alkanes in host cells. In particular, aspects of the invention describe components of genes associated with the difunctional alkane production from carbohydrate feedstocks in host cells. More specifically, aspects of the invention describe metabolic pathways for the production of adipic acid, aminocaproic acid, caprolactam, and hexamethylenediamine via 2-ketopimelic acid. | 11-12-2015 |
20150344919 | PROCESS FOR THE PREPARATION OF PREGABALIN - The present invention provides an improved process for the preparation of a compound of formula (I), which comprises the steps of: formula (I), (a) reacting isovaleraldehyde of formula (II) and alkyl cyanoacetate of formula (III) optionally in presence of salts of weak acid and weak base or weak base in a suitable solvent to get 2-cyano-5-methyl-hex-2-enoic acid alkyl ester of formula (IV); (b) reacting 2-cyano-5-methyl-hex-2-enoic acid alkyl ester of formula (IV) with a suitable cyanide source in water or in an organic solvent or mixture thereof to get 2-isobutylsuccinonitrile of formula (V); (c) obtaining optionally 2-isobutylsuccinonitrile of formula (V) by reacting isovaleraldehyde of formula (II) and alkyl cyanoacetate of formula (III) in presence of suitable cyanide source in water or in an organic solvent or mixture thereof in single step; (d) converting 2-isobutylsuccinonitrile of formula pa (V) to racemic 3-cyano-5-methyl-hexanoic acid or salt thereof of formula (VI) with a genetically modified nitrilase enzyme (Nit pt 9N_56_2) in water or optionally with an organic co-solvent at appropriate pH and temperature; (e) converting racemic 3-cyano-5-methyl-hexanoic acid or salt thereof of formula (VI) to racemic alkyl 3-cyano-5-methyl-hexanoate of formula (VII) by treatment with alcohol (R3OH) and acidic catalyst or alkyl halide (R3X) in presence of a base in a suitable solvent or a mixture of solvents thereof; (f) obtaining (S)-alkyl 3-cyano-5-methyl-hexanoate of formula (VIII) and (R)-3-cyano-5-methyl-hexanoic acid or salt thereof of formula (X) by enzymatic enantioselective hydrolysis in water or organic solvent or a mixture thereof from racemic alkyl 3-cyano-5-methyl-hexanoate of formula (VII); (g) obtaining optionally the compound of formula (VII) by racemizing unwanted (R)-3-cyano-5-methyl-hexanoic acid or salt thereof of formula (X) or substantially enriched (R)-3-cyano-5-methyl-hexanoic acid salt thereof of formula (X) in presence of a base in organic solvent or a mixture thereof; (h) converting (S)-alkyl 3-cyano-5-methyl-hexanoate of formula (VIII) to pregabalin of formula (I) by hydrolyzing ester group with suitable alkali or alkaline earth metal base followed by hydrogenation optionally in one pot in a solvent selected from water or other organic solvents or a mixture thereof in presence of a suitable hydrogenation catalyst. | 12-03-2015 |
20150361458 | METHODS, REAGENTS AND CELLS FOR BIOSYNTHESIZING COMPOUND - This document describes biochemical pathways for producing 2(E)-heptenedioyl-CoA methyl ester from precursors such as 2-oxo-glutarate, acetyl-CoA, or succinyl-CoA using one or more of a fatty acid O-methyltransferase, a thioesterase, a CoA-transferase, a CoA ligase, as well as recombinant hosts expressing one or more of such enzymes. 2(E)-heptenedioyl-CoA methyl ester can be enzymatically converted to pimeloyl-CoA using a trans-2-enoyl-CoA reductase, and a methylesterase. Pimeloyl-CoA can be enzymatically converted to pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol. | 12-17-2015 |
20160010038 | Polypeptides With Lipase Activity and Polynucleotides Encoding Same | 01-14-2016 |
20160032328 | METHODS AND COMPOSITIONS FOR ENHANCED PRODUCTION OF FATTY ALDEHYDES AND FATTY ALCOHOLS - The invention relates to the use of EntD polypeptides, polynucleotides encoding the same, and homologues thereof to enhance the production of fatty aldehydes and fatty alcohols in a host cell. | 02-04-2016 |
20160032334 | METHODS FOR THE SYNTHESIS OF CHIRAL KYNURENINE COMPOUNDS - Provided are methods for synthesizing compounds, including chiral kynurenine compounds. The methods are suitable for large-scale manufacture and produce the chiral kynurenines compounds in high chemical purity and high chiral purity. | 02-04-2016 |
20160032335 | (R)- SELECTIVE AMINATION - The present invention relates to a method for the enzymatic synthesis of enantiomerically enriched (R)-amines of general formula [1][c] | 02-04-2016 |
20160040140 | ENGINEERED IMINE REDUCTASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND AMINE COMPOUNDS - The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds. | 02-11-2016 |
20160040199 | ENZYMATIC METHODS FOR NITROGEN-ATOM TRANSFER - The present invention provides methods for catalyzing a nitrene insertion into a C—H bond to produce a product having a new C—N bond, comprising providing a C—H containing substrate, a nitrene precursor and an engineered heme enzyme; and allowing the reaction to proceed for a time sufficient to form a regioselective product having a new C—N bond. | 02-11-2016 |
20160046969 | METHOD FOR PRODUCING 5-AMINOLEVULINIC ACID OR SALT THEREOF - Provided is a method for producing 5-aminolevulinic acid or a salt thereof at a high yield using 5-aminolevulinic acid-producing microorganisms. The method for producing 5-aminolevulinic acid or a salt thereof comprises culturing 5-aminolevulinic acid-producing microorganisms in a medium comprising one or more components selected from the group consisting of L-arginine, glutamic acid, and a salt thereof. The content of glutamic acid or the salt thereof is from 42 to 100 mM in the medium as the glutamic acid. | 02-18-2016 |
20160068876 | CORYNEFORM BACTERIUM TRANSFORMANT WITH IMPROVED ANILINE PRODUCTIVITY AND PROCESS FOR PRODUCING ANILINE USING THE SAME - An objective of the present invention is to provide a microorganism capable of efficiently producing aniline from aminobenzoic acid, and a process for efficiently producing aniline from aminobenzoic acid. To achieve the objective, provided is an aniline-producing transformant constructed by introducing a gene which encodes an enzyme having aminobenzoate decarboxylase activity into a coryneform bacterium as a host, characterized in that the enzyme having aminobenzoate decarboxylase activity is composed of an amino acid sequence which is the same as that represented by SEQ ID NO: 2 except for having a mutation of at least proline (P) at position 309 from the N terminus. | 03-10-2016 |
20160122788 | CONTINUOUS PRODUCTION METHOD FOR 5-AMINOLEVULINIC ACID BY USING PHOTOSYNTHETIC MEMBRANE VESICLE - A method of continuously producing 5-aminolevulinic acid employs the photosynthetic bacteria-derived photosynthetic membrane vesicle, succinyl-CoA synthetase, and 5-aminolevulinic acid synthase. The enzymatic synthesis of 5-aminolevulinic acid directly from succinic acid and glycine may be simple, but the synthesis is not inexpensive due to the supply of ATP and CoA, which are relatively expensive reactants. The photosynthetic membrane vesicle is used together with succinyl-CoA synthetase and 5-aminolevulinic acid synthase, thereby enabling the re-use of adenosine diphosphate or CoA in reaction. Accordingly, relatively expensive 5-aminolevulinic acid can be efficiently produced at low manufacturing costs from succinic acid and glycine. | 05-05-2016 |
20160130617 | Coryneform Bacterium Transformant and Process for Producing Aniline Using The Same - Provided is an aniline-producing transformant constructed by introducing a gene which encodes an enzyme having aminobenzoate decarboxylase activity into a coryneform bacterium as a host. Also provided is a process for producing aniline, which comprises a step of allowing the transformant to react in a reaction mixture containing aminobenzoic acid, an ester thereof, and/or a salt thereof under reducing conditions, and a step of recovering aniline from the reaction mixture. | 05-12-2016 |
20160138062 | METHOD FOR PRODUCING CATHINE - A method for producing cathine ((1S,2S)-norpseudoephedrine), in which, in a first reaction step, benzaldehyde is reacted with an acetyl donor according to formula (1), where R═H or COOH, by way of an (S)-selective lease to yield an enantiomer mixture according to formulas (2) and (3) and, in a second step, the compound according to formula (3) is reacted with an amine donor by way of an (S)-selective transaminase to yield (1S,2S)-norpseudoephedrine. | 05-19-2016 |
20160152548 | NOVEL INTERMEDIATE FOR SYNTHESIZING TREPROSTINIL DIETHANOLAMINE AND METHOD FOR PREPARING THE SAME | 06-02-2016 |
20160168545 | ENGINEERED IMINE REDUCTASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND AMINE COMPOUNDS | 06-16-2016 |
20160168605 | A NOVEL MODIFIED ORNITHINE DECARBOXYLASE PROTEIN AND A USE THEREOF | 06-16-2016 |
20160177346 | METHOD FOR TREATING SUGAR SOLUTION, HYDROGENATED SUGAR SOLUTION, METHOD FOR PRODUCING ORGANIC COMPOUND, AND METHOD FOR CULTURING MICROORGANISMS | 06-23-2016 |
20160201048 | STABILIZED RECOMBINANT EXPRESSION PLASMID VECTOR IN HAFNIA ALVEI AND APPLICATIONS THEREOF | 07-14-2016 |
20160251478 | ENZYMATIC SYNTHESIS OF POLY(AMINE-CO-ESTERS) AND METHODS OF USE THEREOF FOR GENE DELIVERY | 09-01-2016 |
20160376580 | Method of Producing 1,5-Pentadiamine Using Lysine Decarboxylase Mutant Having Improved Thermal Stability - The present invention provides methods for producing 1,5-pentamethylenediamine (“1,5-PD”) efficiently in a manner suitable for an actual production. Specifically, the present invention provides a method of producing 1,5-pentamethylenediamine including allowing a lysine decarboxylase mutant to act on L-lysine and/or a salt thereof, wherein said lysine decarboxylase mutant has an amino acid sequence selected from the group consisting of:
| 12-29-2016 |
20180023102 | MATERIALS AND METHODS UTILIZING BIOTIN PRODUCING MUTANT HOSTS FOR THE PRODUCTION OF 7-CARBON CHEMICALS | 01-25-2018 |