| Entries |
| Document | Title | Date |
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| 20080213841 | Novel Method for Assembling DNA Metasegments to use as Substrates for Homologous Recombination in a Cell - The invention relates to a novel method for obtaining DNA metasegment comprising ligating adjoining DNA fragments at least 10 Kb in size containing at least one overlapping region. | 09-04-2008 |
| 20080241889 | METHODS AND COMPOSITIONS FOR SYNTHESIS OF NUCLEIC ACID MOLECULES USING MULTIPLE RECOGNITION SITES - The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention. | 10-02-2008 |
| 20080261275 | Cdna Production from Cells After Laser Microdissection - The present invention provides a new procedure for the synthesis of CDNA from single cells after microdissection. It has the advantage that it is cost-efficient and can be carried out quickly with only few steps, even by less skilled laboratory employees. For the first time, the time-consuming and risky step of RNA isolation is omitted during cDNA synthesis from single cells by performing lysis and cDNA synthesis in the same reaction tube and in one buffer solution, which provides reliable and contamination-free results. The buffer is composed of NP40, carrier-RNA and Super RNAsin, as well as dNTPs and cDNA synthesis primers. | 10-23-2008 |
| 20080274510 | Synthetic genes - The invention provides strategies, methods, vectors, reagents, and systems for production of synthetic genes, production of libraries of such genes, and manipulation and characterization of the genes and corresponding encoded polypeptides. In one aspect, the synthetic genes can encode polyketide synthase polypeptides and facilitate production of therapeutically or commercially important polyketide compounds. | 11-06-2008 |
| 20080280329 | Enzymatic synthesis of deoxyribonucleosides - The present invention relates to a method for the in vitro synthesis of deoxyribonucleosides and enzymes suitable for this method. | 11-13-2008 |
| 20080299621 | MINIPREP SYSTEM FOR SIMPLE AND RAPID PLASMID DNA EXTRACTION - The invention relates to a modified microspin system for the isolation and purification of plasmid DNA. A disposable pre-filter column is used in combination with the traditional microspin column for increase speed and quality of plasmid DNA preparation. The disposable pre-filter column includes a pre-filter and a depth filter for optimal result. During plasmid DNA isolation, the lysate can be loaded directly to the assembly including the disposable pre-filter column and the microspin column. A quick spin causes the plasmid DNA to bind to the microspin column while the flocculents remain on top of the disposable pre-filter column, eliminates the need to first remove the flocculants containing cellular debris. This results in a much shortened process. Also provided are kits for isolation of plasmid DNA including the pre-filter column and the microspin columns. | 12-04-2008 |
| 20080318279 | APPARATUS AND METHOD FOR PURIFICATION OF NUCLEIC ACIDS BY PHASE SEPARATION USING LASER AND BEADS - An apparatus and method for purification of nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples and magnetic beads are introduced; a vibrator attached to the capillary and mixing the samples and the magnetic beads in the capillary; a laser generator attached to the capillary and supplying a laser to the capillary; and a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall. According to the method and apparatus, PCR yield can be increased since PCR inhibitors can be readily removed by means of a phase separation in a capillary. The use of an electromagnet ensures the removal of the PCR inhibitors. In addition, since cell lysis and DNA purification process can be simultaneously performed, LOC steps can be reduced. | 12-25-2008 |
| 20090004700 | Chemical Induction in Quiescence in Bacteria - Quiescence is induced in cells using indole compounds. Expression continues from extra-chromosomal vectors within the cells during quiescence, while chromosomal expression is suppressed. The cells may be used as factories for the production of large amounts of polypeptides of interest, particularly polypeptides which normally have an adverse effect on cell viability or growth. Expression from an extra-chromosomal vector of interest may be monitored, in view of the reduced background expression from the chromosome. Vector copy number may be amplified. Cell cycles may be synchronized. | 01-01-2009 |
| 20090011469 | METHOD AND FORMULATION FOR THE EXTRACTION OF NUCLEIC ACIDS FROM ANY COMPLEX STARTING MATERIALS - The invention relates to a universal and greatly simplified method as well as a composition for isolating nucleic acids from different starting materials containing nucleic acids. The composition contains at least one buffer solution for proteolytically solubilizing biological samples, the buffer containing no chaotropic or antichaotropic component, at least one alcoholic component and/or a detergent, a solid phase, and a wash and elution buffer. | 01-08-2009 |
| 20090061487 | SIRNA AND METHODS OF MANUFACTURE - Double-stranded RNA of about 19 to about 25 nucleotides in length capable of regulating gene expression by RNA interference is provided. Such double-stranded RNA are particularly useful for treating disease or conditions associated with a target mRNA or gene. Methods of manufacture and methods of use of the double-stranded RNA are also provided. | 03-05-2009 |
| 20090068707 | PLASMID DNA PREPARATIONS AND METHODS FOR PRODUCING SAME - The various embodiments of the present invention relate generally to plasmid DNA preparations and to methods for producing and using such preparations. | 03-12-2009 |
| 20090081736 | Methods For Using Mutant RNA Polymerases Wtih Reduced Discrimination Between Non-Canonical Nucleoside Triphosphates - A method for synthesizing a nucleic acid molecule comprising at least one non-canonical nucleoside triphosphate using a mutant polymerase having a reduced discrimination between canonical and non-canonical substrates is disclosed. The method comprises incubating a template nucleic acid in a reaction mixture comprising the mutant nucleic acid polymerase and the appropriate canonical and non-canonical nucleoside triphosphates which are desired substrates for the mutant nucleic acid polymerase. The present invention is also a method of determining the sequence of a nucleic acid molecule using the mutant polymerase to create a nucleic acid molecule comprising at least one non-canonical nucleoside triphosphate. | 03-26-2009 |
| 20090081737 | METHODS AND COMPOSITIONS FOR REDUCING THE COMPLEXITY OF A NUCLEIC ACID SAMPLE - Aspects of the present invention are drawn to methods and compositions for sorting nucleic acid molecules into physically separate compartments according to the identity of a nucleotide base or sequence of bases at a specific location, resulting in the production of reduced complexity samples that find use in any number of downstream genetic analyses. Aspects of the methods of the invention include fragmenting a nucleic acid sample, e.g., with a restriction enzyme, ligating an adaptor (or adaptors), and sorting the fragments based on the identity of the nucleotide base(s) positioned adjacent to the fragmentation site (e.g., the restriction enzyme cut site/or recognition site). Each round of sorting produces binned samples having reduced complexity over the parent sample. | 03-26-2009 |
| 20090098611 | Self-cleaving affinity tags and methods of use - The present invention relates to compositions and methods relating to development of modified inteins comprising one or more topoisomerase recognition sequences and the corresponding topoisomerase proteins and/or one or more recombination sites and the corresponding recombination proteins systems for use in affinity-based protein expression systems. | 04-16-2009 |
| 20090117620 | AUTOMATED ANALYZER FOR CLINICAL LABORATORY - A laboratory automation system that is capable of carrying out clinical chemistry assays, immunoassays, amplification of nucleic acid assays, and any combination of the foregoing, said laboratory automation system employing at least one of micro-well plates and deep multi-well plates as reaction vessels. The use of micro-well plates as reaction vessels enables the laboratory automation system to assume a variety of arrangements, i.e., the laboratory automation system can comprise a variety of functional modules that can be arranged in various ways. In order to effectively carry out immunoassays by means of micro-well plates, a technique known as inverse magnetic particle processing can be used to transfer the product(s) of immunoassays from one micro-well of a micro-well plate to another. | 05-07-2009 |
| 20090148909 | GENE SMS 27 - The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C. | 06-11-2009 |
| 20090148910 | REUSABLE PCR AMPLIFICATION SYSTEM AND METHOD - A DNA amplification device utilizing a polydimethylsiloxane (PDMS) and silicon substrate coated with spin-on glass (SOG) is provided. This PDMS layer is irreversibly bonded to the SOG layer of the silicon substrate using oxygen plasma. The amplification device is an inexpensive, microfluidic device, which can be utilized as a portable thermo-cycler to perform PCR amplification of DNA in the field. | 06-11-2009 |
| 20090155853 | NON-VIRAL GENE DELIVERY COMPLEX - The invention relates to fusion proteins useful in delivering a targeted nucleic acid to a target cell, comprising a gene delivery fusion protein (GDFP), said GDFP comprising a nucleic acid binding domain (NBD) that binds to the targeted nucleic acid, fused to a gene delivery domain (GDD) that mediates delivery of the targeted nucleic acid to the target cell, wherein said GDD comprises one or more components that facilitate delivery of a targeted nucleic acid to a target cell, and wherein one of said components is a transport/localization component and wherein said transport/localization component is an adenovirus protein V or derivative thereof that retains protein V activity, and related methods of making and using thereof. | 06-18-2009 |
| 20090176279 | Bioreactive agents - This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. Useful compositions include biological agents such as nucleic acids, proteins, lipids and cytokines. Conjugates can also be used to monitor the pathway and half-life of pharmaceutical composition in vivo and for diagnostic, therapeutic and prophylactic purposes. The invention also relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and nearly any individual substance in a complex background of substances. | 07-09-2009 |
| 20090186385 | RECOMBINATIONAL CLONING USING NUCLEIC ACIDS HAVING RECOMBINATION SITES - Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s). | 07-23-2009 |
| 20090186386 | RECOMBINATIONAL CLONING USING NUCLEIC ACIDS HAVING RECOMBINATION SITES - Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s). | 07-23-2009 |
| 20090203081 | INDUCIBLE EXPRESSION VECTORS AND METHODS OF USE THEREOF - The present invention provides nucleic acids that include a promoter that is inducible by a transcriptional activator protein; and a nucleotide sequence that encodes the transcriptional activator protein. The present invention provides expression vectors that provide for inducible production of gene products in a host cell. The present invention further provides host cells genetically modified with a subject expression vector. The present invention further provides methods for producing a gene product in a host cell. | 08-13-2009 |
| 20090209007 | Method of Expression Cloning in a Host Cell - The invention relates to a promoter DNA sequence highly suited in an improved expression cloning method for isolation of DNA sequences comprising a DNA sequence encoding a protein of interest in a host cell and to the improved expression cloning method wherein use is made of this promoter. The isolated DNA sequences are useful in processes for producing a protein of interest. | 08-20-2009 |
| 20090215123 | Method for Producing Continuous Cell Lines - The present invention relates to a method for production of continuous cell lines comprising providing living cells of an animal or a human, irradiating said cells with UV light, proliferating said cells and selecting multiplying cells as cells of a continuous cell line. | 08-27-2009 |
| 20090239265 | VIRUS LIKE PARTICLES - An isolated polynucleotides comprising a HBsAg-S coding sequence that is adapted to receive an insert coding sequence, within a part of the HBsAg-S coding sequence that encodes an exposed site within the external loop of HBsAgS, and still encode a HBsAg-S that is able to assemble into a VLP. Proteins encoded by the polynucleotides, recombinant VLP's and various uses thereof are also described. | 09-24-2009 |
| 20090246833 | Small interfering RNAS as non-specific drugs - The present invention is directed to a method of modulating (e.g., inducing, inhibiting) activation of a double stranded RNA (dsRNA) signaling pathway, such as the dsRNA signaling pathway that accompanies RNA interference (RNAi) of a target RNA sequence, in a cell, comprising introducing into the cell small interfering RNA (siRNA) that degrades the target RNA sequence, and maintaining the cell under conditions in which RNAi of the target RNA sequence occurs and activation of the dsRNA signaling pathway is modulated in the cell. | 10-01-2009 |
| 20090253181 | Universal sample preparation system and use in an integrated analysis system - The invention provides for devices and methods for interfacing microchips to cartridges and pneumatic manifolds. The cartridges, microchips, and pneumatic manifolds can be integrated with downstream preparation devices, such as thermal regulating devices and separation and analysis devices. | 10-08-2009 |
| 20090253182 | FED-BATCH FERMENTATION PROCESS AND CULTURE MEDIUM FOR THE PRODUCTION OF PLASMID DNA IN E. COLI ON A MANUFACTURING SCALE - A process for producing plasmid DNA | 10-08-2009 |
| 20090263868 | Nucleotide Compositions Comprising Photocleavable Markers And Methods Of Preparation Thereof - Labelled nucleotides and polynucleotides useful in the sequencing of nucleic acids are described. Methods of preparing photocleavable marker nucleotides and photocleavable marker-polynucleotide conjugates are described. Such photocleavable marker nucleotides can be incorporated into nucleic acid so as to create photocleavable marker-polynucleotide conjugates. | 10-22-2009 |
| 20090275086 | ASSEMBLY OF LARGE NUCLEIC ACIDS - A method to assemble any desired nucleic acid molecule by combining cassettes in vitro to form assemblies which are further combined in vivo, or by assembling large numbers of DNA fragments by recombination in a yeast culture to obtain desired DNA molecules of substantial size is described. | 11-05-2009 |
| 20090291474 | COLLAGEN-LIKE POLYPEPTIDES AND ENCODING POLYNUCLEOTIDES - Provided herein are isolated polynucleotides encoding collagen-like polypeptide domains and the encoded polypeptides. In one such polypeptide, at least a portion of the encoded collagen-like polypeptide domain is in the form of Gly-X-Y triads, where X and Y symbolize individual amino acids; X is proline in at least 20% of the triads; Y is proline in at least 20% of the triads. The encoding polynucleotide of such a polypeptide encodes a region having at least 50 consecutive amino acids, wherein the region is at least 90% identical to an amino acid sequence of a naturally occurring collagen polypeptide; and no more than 98% of the nucleotides of the collagen-like polypeptide domain-encoding portion of the polynucleotide fall into a window of 100 consecutive nucleotides in which the nucleotides of the window have greater than 98% identity to a naturally occurring collagen-encoding nucleotide sequence. | 11-26-2009 |
| 20090298128 | Method for Amplifying Unknown DNA Sequence Adjacent to Known Sequence - The present invention relates to a method for amplifying an unknown nucleotide sequence adjacent to a known nucleotide sequence, which comprises the step of (a) performing a primary amplification of the unknown nucleotide sequence using a DNA walking annealing control primer (DW-ACP) and a first target specific primer (TSP) hybridizable with a site on the known nucleotide sequence; in which the step (a) comprises: (a-1) performing a first-stage amplification of the unknown nucleotide sequence at a first annealing temperature, using a first degenerate DW-ACP containing (i) a degenerate random nucleotide sequence to hybridize with the unknown nucleotide sequence and (ii) a hybridizing nucleotide sequence substantially complementary to a site on the unknown nucleotide sequence, wherein the first annealing temperature enables the first degenerate DW-ACP to function as a primer, whereby a first degenerate DW-ACP extension product is generated; and (a-2) performing a second-stage amplification of the amplification product generated from step (a-1) at a second annealing temperature which is higher than the first annealing temperature, using the first degenerate DW-ACP as used in the step (a-1) and the first TSP, under conditions in which each primer anneals the its target nucleotide sequence, whereby a primary amplification product is generated. | 12-03-2009 |
| 20090311753 | Method for amplifying genomic DNA - A method for amplifying genomic DNA is provided. The method comprises the steps of: (1) incubating a cell-containing agarose solution at a pH of 9 to 12 and a temperature of 45 to 80° C. to produce a genomic DNA-dispersed agarose solution wherein 0.002 to 1 copies/5 microliter of single-stranded genomic DNA is dispersed; (2) solidifying the genomic DNA-dispersed agarose solution to produce a genomic DNA-dispersed agarose gel and neutralizing a pH of the gel; and (3) adding a DNA polymerase with strand displacement activity, primer and dNTP to the genomic DNA-dispersed agarose gel and incubating the gel at a temperature of 0 to 60° C. to amplify the genomic DNA. | 12-17-2009 |
| 20090317873 | Design, synthesis and assembly of synthetic nucleic acids - Methods of synthesizing oligonucleotides with high coupling efficiency (>99.5%) are provided. Methods for purification of synthetic oligonucleotides are also provided. Instrumentation configurations for oligonucleotide synthesis are also provided. Methods of designing and synthesizing polynucleotides are also provided. Polynucleotide design is optimized for subsequent assembly from shorter oligonucleotides. Modifications of phosphoramidite chemistry to improve the subsequent assembly of polynucleotides are provided. The design process also incorporates codon biases into polynucleotides that favor expression in defined hosts. Design and assembly methods are also provided for the efficient synthesis of sets of polynucleotide variants. Software to automate the design and assembly process is also provided. | 12-24-2009 |
| 20090325233 | METHOD OF PRODUCING A NUCLEIC ACID - The present invention provides a method of producing a nucleic acid at high reaction efficiency and high reproducibility with a decreased variation in yield and purity among different reaction lots. A nucleic acid synthesis reaction is carried out on a first solid phase carrier capable of supporting nucleic acid synthesis contained in a solid phase carrier mixture comprising the first solid phase carrier and a second solid phase carrier that does not support nucleic acid synthesis. | 12-31-2009 |
| 20100015666 | ISOTHERMAL DNA AMPLIFICATION - The present invention provides for amplification of one or more polynucleotides by multi-staged linear amplifications using one or more RNA polymerases. At each stage RNA transcripts are accumulated at a linear rate, so that multiple stages provide for faster than linear transcript accumulation. In one aspect, the invention provides for polynucleotide amplification by ligating hairpin adaptors to an end of polynucleotides wherein the hairpin adaptors each contain a promoter sequence oriented so that transcription proceeds in the direction of the loop of the hairpin adaptor. Upon transcription through such loop region and to the complementary strand a replicate is made of the promoter sequence as well as the polynucleotide, thereby permitting exponential amplification upon reverse transcription, second strand synthesis, and repetition of the above cycle. Preferably such amplification is carried out under isothermal reaction conditions. | 01-21-2010 |
| 20100028953 | Methods for production of oligonucleotides - The present invention relates to production of oligonucleotides using rolling circle replication, wherein synthesised multimeric oligonucleotides are reduced to mononucleotides using a nicking cassette. Thus, the invention provides a method for the production of oligonucleotides, enabling efficient amplification of oligonucleotides at lengths up to at least 1000 nucleotides and in high amounts contained within a nicking cassette. | 02-04-2010 |
| 20100047875 | Making nucleic acid sequences in parallel and use - The present invention relates generally to the fields of genomics, synthetic biology and genetic engineering. More particularly, the present invention concerns the methods that enable parallel multiplex ligation and amplification on surface for making assemblies of nucleic acids of various biological applications and for analysis of biological samples such as DNA, RNA, and proteins. | 02-25-2010 |
| 20100062493 | METHOD FOR PRODUCING AN L-AMINO ACID OR A NUCLEIC ACID - A method is described for producing an L-amino acid or a nucleic acid by culturing a microorganism having an ability to produce the L-amino acid or nucleic acid in a liquid medium in a fermentation tank containing a stirring impeller, and optionally adding seed crystals to the medium as required to produce and accumulate crystals of the L-amino acid or nucleic acid in the medium, and collecting crystals of the L-amino acid or nucleic acid from the culture. The power density of the stirring impeller is controlled to be 2.4 kW/m | 03-11-2010 |
| 20100075382 | SSB-POLYMERASE FUSION PROTEINS - Fusion proteins comprising a single strand DNA binding protein and a nucleic acid polymerase (e.g. DNA polymerase or reverse transcriptase). These high fidelity proteins are suitable for use in nucleic acid amplification methods, including the polymerase chain reaction (PCR). | 03-25-2010 |
| 20100075383 | METHODS FOR AMPLIFYING POLYMERIC NUCLEIC ACIDS - The invention provides compositions and methods for amplifying nucleic acid polymer sequences in a high complexity nucleic acid sample. The unique compositions of the invention include a primer set composed of a mixture of two types of primers for DNA synthesis. For extension in one direction, the primers all contain modifications that destroy their ability to serve as templates that can be copied by DNA polymerases. For extension in the opposite direction the set includes at least one primer that can serve as a template and be replicated by DNA polymerases throughout its length. The method can be carried out by mixing the nucleic acid polymer sequence of interest with the set of DNA synthesis primers in an amplification reaction mixture. The reaction mixture is then subjected to temperature cycling analogous to the temperature cycling in PCR reactions. At least one primer in the primer set hybridizes to the nucleic acid polymer. It is preferred that the non-replicable primer hybridizes to the nucleic acid polymer and is extended to produce an extension product that contains sequence from the nucleic acid polymer to which the replicable primer then hybridizes. Of course, if the nucleic acid polymer is double stranded, both the replicable and nonreplicable primers will hybridize and be extended by DNA polymerase. | 03-25-2010 |
| 20100120097 | METHODS AND COMPOSITIONS FOR NUCLEIC ACID SEQUENCING - The present invention includes novel compositions and method for nucleic acid sequencing. The methods and compositions permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number of different oligonucleotides with superior output and quality. | 05-13-2010 |
| 20100129870 | METHOD FOR OBTAINING OLIGONUCLEOTIDE - The present invention is to provide a method for obtaining an oligonucleotide such as RNA aptamer having high binding capacity to a target substance with easy-to-use and high purity. The method for obtaining oligonucleotide according to the present invention is as follows: | 05-27-2010 |
| 20100129871 | PYROPHOSPHOROLYSIS ACTIVATED POLYMERIZATION (PAP) - A novel method of pyrophosphorolysis activated polymerization (PAP) has been developed. In PAP, pyrophosphorolysis and polymerization by DNA polymerase are coupled serially for each amplification by using an activatable oligonucleotide P* that has a non-extendible 3′-deoxynucleotide at its 3′ terminus. PAP can be applied for exponential amplification or for linear amplification. PAP can be applied to amplification of a rare allele in admixture with one or more wild-type alleles by using an activatable oligonucleotide P* that is an exact match at its 3′ end for the rare allele but has a mismatch at or near its 3′ terminus for the wild-type allele. PAP is inhibited by a mismatch in the 3′ specific sequence as far as 16 nucleotides away from the 3′ terminus. PAP can greatly increase the specificity of detection of an extremely rare mutant allele in the presence of the wild-type allele. Specificity results from both pyrophosphorolysis and polymerization since significant nonspecific amplification requires the combination of mismatch pyrophosphorolysis and misincorporation by the DNA polymerase, an extremely rare event. Using genetically engineered DNA polymerases greatly improves the efficiency of PAP. | 05-27-2010 |
| 20100129872 | SYSTEM AND METHOD FOR MOVEMENT AND POSITIONING OF REACTION MIXTURE DURING NUCLEIC ACID AMPLIFICATION - A system for accurately positioning a reaction mixture during amplification of a nucleic acid includes a reaction vessel that contains the reaction mixture, a first heat exchanger, a second heat exchanger and a pump assembly. The reaction vessel can include .a first zone and a second zone. The first heat exchanger is positioned near the first zone, and the second heat exchanger positioned near the second zone. The first heat exchanger adjusts the temperature of the reaction mixture so the reaction mixture is at a first temperature when the reaction mixture is in the first zone. The second heat exchanger adjusts the temperature of the reaction mixture so the reaction mixture is at a second temperature. The pump assembly adjusts the pressure within the reaction vessel to selectively position the reaction mixture relative to the first zone and the second zone during amplification. The system can include a sensor that monitors the position of the reaction mixture within the reaction vessel. The system can also include a second pump that cooperates with the pump assembly to adjust the position of the reaction mixture within the reaction vessel. | 05-27-2010 |
| 20100129873 | Highly Parallel Gel-Free Cloning Method - A highly parallel method for gene cloning is presented. PCR products can be isolated using a solid phase and ligated into a positive selection vector. The cloning method has a very high success rate and can be performed entirely by a liquid handling robot with very little human intervention. | 05-27-2010 |
| 20100151530 | METHOD FOR PRODUCING PLASMID DNA ON A MANUFACTURING SCALE - A method for producing plasmid DNA on a manufacturing scale uses | 06-17-2010 |
| 20100159524 | APPARATUS AND METHODS FOR PRODUCING AND USING HIGH-DENSITY CELLS AND PRODUCTS THEREFROM - Disclosed and claimed is apparatus and methods for the growth of cells to high density, products therefrom and uses thereof. Also disclosed and claimed is the use of this method for the growth to high-density insect cells, such as the | 06-24-2010 |
| 20100159525 | Extranuclear RNA Splicing in Neuronal Dendrites - The present invention relates to methods of synaptic network remodeling by means of extranuclear RNA splicing. The present invention also provides methods of extranuclear RNA splicing, and methods of protein translation based on extranuclear RNA splicing. | 06-24-2010 |
| 20100159526 | SELECTIVE 5' LIGATION TAGGING OF RNA - The present invention provides novel compositions, kits and methods employing RNA 5′ polyphosphatases, RNA 5′ monophosphatases, capping enzymes, decapping enzymes, nucleic acid pyrophosphatases and RNA ligases, as well as other enzymes, for selective 5′ ligation tagging of desired classes of RNA molecules that differ with respect to particular chemical moieties on their 5′ ends. The 5′tagged RNA molecules can be used for synthesis of tagged first-stand cDNA, double-stranded cDNA, and sense or antisense RNA for a variety of uses. | 06-24-2010 |
| 20100167352 | Infectious clones - The present invention relates to methods of preparing a DNA comprising steps, wherein (a) a DNA comprising a full length copy of the genomic RNA (gRNA) or an RNA virus; or (b) a DNA comprising one or several fragments of a gRNA of an RNA virus, which fragments code for an RNA dependent RNA polymerase and at least one structural or non-structural protein; or (c) a DNA having a homology of at least 60% to the sequences of (a) or (b); is cloned into a bacterial artificial chromosome (BAC). Additionally, DNAs are provided, which comprise sequences derived from the genomic RNA (gRNA) of a coronavirus which sequences have a homology of at least 60% to the natural sequence of the virus and code for an RNA dependent RNA polymerase and at least one structural or no-structural protein, wherein a fragment of said DNA is capable of being transcribed into RNA which RNA can be assembled to a virion. Further, the use of these nucleic acids for preparation of viral RNA or virions as well as pharmaceutical preparations comprising these DNAs, viral RNAs or virions is disclosed. | 07-01-2010 |
| 20100196965 | Acidophilic Enzymes - The present invention relates to enzymes having catalytic activity at a pH below 5.0. The present invention provides hydrolyzing enzymes obtainable from archaeobacteria, in detail to hydrolytic enzymes obtainable from the archaeobacterium | 08-05-2010 |
| 20100216192 | TAILORED MULTI-SITE COMBINATORIAL ASSEMBLY - The present invention provides a novel method of producing a plurality of modified polynucleotides having different combinations of various mutations at multiple sites by a tailored multi-site combinatorial assembly, comprising adding at least two or at least three primers to a double stranded template polynucleotide in a single reaction mixture, wherein the primers are not overlapping, and wherein each of the primers comprise at least one mutation different from the other primers, wherein at least one primer is a forward primer that can anneal to a minus strand of the template and at least one primer is a reverse primer that can anneal to a plus strand of the template, and subjecting the reaction mixture to a polymerase extension reaction to yield a plurality of extended modified polynucleotides from the at least three primers. The method can be performed without employing a ligation step prior to transforming the extended modified polynucleotides into a cell. The plurality of extended modified polynucleotides can be treated with an enzyme for destroying the template polynucleotide prior to transforming in to the cell. | 08-26-2010 |
| 20100255545 | INTEGRATED - LIGAND-RESPONSIVE MICRORNAS - The present application relates to nucleic acids that encode an miRNA and a sensor domain that can respond to a ligand. In some embodiments, the sensor domain is an RNA aptamer that modulates processing of the miRNA by an RNA processing enzyme, for example, Drosha. | 10-07-2010 |
| 20100261228 | MULTIPLEXED SITES FOR POLYMER SYNTHESIS - Disclosed herein are methods, devices, and other components for synthesizing polymers. In some embodiments, numerous sites can be multiplexed together to allow for effective nucleic acid synthesis. | 10-14-2010 |
| 20100291633 | METHOD OF CLONING AT LEAST ONE NUCLEIC ACID MOLECULE OF INTEREST USING TYPE IIS RESTRICTION ENDONUCLEASES, AND CORRESPONDING CLONING VECTORS, KITS AND SYSTEM USING TYPE IIS RESTRICTION ENDONUCLEASES - The present invention refers to methods of (sub)cloning at least one nucleic acid molecule of interest. One embodiment relates to a method of (sub)cloning at least one nucleic acid molecule of interest comprising a) providing at least one (replicable) Entry vector into which the at least one nucleic acid molecule of interest is to be inserted, wherein the at least one Entry vector carries two recognition sites for at least one first type IIS and/or type IIS like restriction endonuclease and wherein said at least one nucleic acid molecule of interest can be excised from the at least one Entry vector at two combinatorial sites with one (same) or more (different) cohesive ends that are formed by the at least one first type IIS or type IIS like restriction endonuclease, and b) providing an Acceptor vector, into which the at least one nucleic acid molecule of interest is transferred from the at least one Entry vector carrying the at least one nucleic acid molecule of interest, wherein said Acceptor vector comprises at least one recognition site for at least one second type IIS restriction endonuclease and/or at least one recognition sites for at least one type IIS like restriction endonuclease, and wherein said Acceptor vector provides two combinatorial sites identical to the two combinatorial sites present in the Entry vector. The inventions also relates respective cloning vector and kits. | 11-18-2010 |
| 20100317060 | METHOD FOR HIGHLY AMPLIFYING TARGET GENE IN MAMMALIAN CELL AND VECTOR THEREFOR - A vector of the present invention is a vector for amplifying a target gene in a mammalian cell, the vector including an amplification-activating fragment, which is a partial fragment of a mammalian replication initiation region and has a gene amplification activity site, and a mammalian nuclear matrix attachment region. In the case where the mammalian replication initiation region as described above derives from a c-myc locus, for example, the above-described partial fragment at least contains a duplex unwinding element and a topoisomerase II-binding domain. The vector as described above improves gene transfer efficiency and gene amplification efficiency compared with the existing high gene amplification systems. Thus, a method whereby a “high gene amplification system” developed by the inventors can amplify a target gene with better gene transfer efficiency and a vector to be used in this method are provided. | 12-16-2010 |
| 20100317061 | NOVEL DNA CLONING METHOD RELYING ON THE E.COLI recE/recT RECOMBINATION SYSTEM - The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described. | 12-16-2010 |
| 20100323403 | POLYPURINE TRACT MODIFIED RETROVIRAL VECTORS - An integration-defective retroviral vector transfer cassette lacking a functional polypurine tract (PPT) is provided. Also provided are isolated nucleic acids that include a heterologous nucleotide sequence, one or two retroviral long terminal repeats (LTRs), a packaging signal, a rev responsive element, and a eukaryotic promoter, wherein the nucleic acid lacks a functional PPT; vectors that include the disclosed isolated nucleic acids; recombinant retroviral particles and mRNAs thereof; retroviral vector kits; and methods for producing integration-defective vector particles, achieving gene expression of a nucleotide sequence of interest, and inserting a nucleotide sequence of interest into a host cell genome in a site-specific or non-specific manner. | 12-23-2010 |
| 20110014657 | Method for sequencing a polynucleotide template - The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template. | 01-20-2011 |
| 20110081684 | METHOD FOR CARRYING OUT AN ENZYMATIC REACTION - The invention relates to a method for carrying out an enzymatic reaction, in particular for carrying out a polymerase chain reaction (PCR). Said method consists of the following steps: at least one eukaryotic cell is removed from a starting material; the cell core or cores of the eukaryotic cell(s) is/are coloured; at least one eukaryotic cell is deposited on a reaction point of a solid substrate in a liquid volume of less than 10 μl; it is detected whether at least one coloured cell core is present on a reaction point of the substrate, subsequently, an enzyme and optionally a reaction buffer is added to the eukaryotic cell(s) and the enzymatic reaction is subsequently started. Preferably, the claimed invention is carried out using a flow cytometer. | 04-07-2011 |
| 20110097761 | MUTANT MUSCLE-SPECIFIC ENHANCERS - The present invention relates to nucleic acid compositions and expression systems comprising muscle-specific regulatory elements, and methods for expressing heterologous DNA sequences in cells. In particular, the present invention provides mutant muscle-specific enhancers, genetic cassettes, and vectors useful in gene therapy, diagnostic assays, and other gene expression systems. | 04-28-2011 |
| 20110117606 | NRPS-PKS Gene Cluster and its Manipulation and Utility - The present invention provides a nucleic acid molecule comprising: (a) a nucleotide sequence as shown in SEQ ID No. 1; or (b) a nucleotide sequence which is the complement of SEQ ID No. 1; or (c) a nucleotide sequence which is degenerate with SEQ ID No. 1; or (d) a nucleotide sequence having at least 85% sequence identity (preferably at least 87%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity) with SEQ ID No. 1; or (e) a part of any one of (a) to (d), wherein said nucleic acid molecule encodes or is a complementary to a nucleic acid molecule encoding one or more polypeptides, or comprises or is complementary to a nucleic acid molecule comprising one or more genetic elements, having functional activity in the synthesis of a polyketide-based or macrolactam molecule. Particularly the invention contemplates the modification of the nucleic acid of the invention, encoding the biosynthetic machinery for the synthesis of the polyketide macrolactam BE-14106, including expressing in a microorganism the modified nucleic acid molecule. In certain aspects the modification includes introducing, mutating, deleting, replacing or inactivating a sequence encoding one or more activities or proteins encoded by said nucleic acid molecule. Other aspects of the invention include a microorganism containing the modified and unmodified nucleic acid and recovering the polyketide-based or macrolactam molecule from said mircoorganism. | 05-19-2011 |
| 20110136178 | BASE-MODIFIED PRIMER OLIGOMERS FOR MULTIPLEX RT-PCR - The invention relates to a method for the amplification of nucleic acid sequences by enzymatic DNA polymerase activity, wherein synthetic oligodeoxynucleotide compounds are used as primers, comprising sequences having the formula (I), wherein the sequence SeqA-Ni-SeqC has at least a length of 8 nucleotides and can hybridize with one of the nucleic acid sequences to be amplified, SeqA and SeqC are sequences of naturally occurring deoxyribonucleotide building blocks or the synthetic analogs thereof, N1 is a phosphoribonucleotide group covalently bound to R1 or a nucleotide analog covalently bound to R1, R1 is a molecule group, comprising at least ten atoms having an atomic weight of twelve and higher, and to reaction mixtures for carrying out the method. | 06-09-2011 |
| 20110151518 | METHOD FOR MODIFYING CHROMOSOMES - The present invention relates to a method for producing a modified foreign chromosome(s) or a fragment(s) thereof, which comprises the steps of: (a) preparing a microcell comprising a foreign chromosome(s) or a fragment(s) thereof, and transferring said foreign chromosome(s) or a fragment(s) into a cell with high homologous recombination efficiency through its fusion with said microcell; (b) in said cell with high homologous recombination efficiency, inserting a targeting vector by homologous recombination into a desired site of said foreign chromosome(s) or a fragment(s) thereof, and/or a desired site of a chromosome(s) derived from said cell with high homologous recombination efficiency, thereby marking said desired site; and (c) in said cell with high homologous recombination efficiency, causing deletion and/or translocation to occur at the marked site of said foreign chromosome(s) or a fragment(s) thereof. | 06-23-2011 |
| 20110171692 | PROCESS FOR AMPLIFYING DNA IN CELLS - The present invention relates to a process for efficiently amplifying a giant DNA. More particularly, the present invention relates to a process for amplifying DNA in a cell, comprising amplifying the DNA as the target of amplification in the presence of DNAs selected from the following (i), (ii) and (iii):
| 07-14-2011 |
| 20110201055 | Engineered cleavage half-domains - Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence. | 08-18-2011 |
| 20110229938 | Method For Amplifying Locus In Bacterial Cell - Certain aspects of this disclosure relate to a method of amplifying a genomic locus. In certain embodiments, the method may comprise: a) contacting a population of bacterial host cells with an inhibitor of an essential enzyme, where the bacterial host cells comprise a genomic locus of the structure: A | 09-22-2011 |
| 20110256589 | T7 RNA POLYMERASE VARIANTS WITH ENHANCED THERMOSTABILITY - The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous. | 10-20-2011 |
| 20110269190 | BARRIERS FOR FACILITATING BIOLOGICAL REACTIONS - The present invention relates to systems, devices, and methods for performing biological reactions. In particular, the present invention relates to the use of lipophilic, water immiscible, or hydrophobic barriers in sample separation, purification, modification, and analysis processes. | 11-03-2011 |
| 20110275124 | Reagents for reversibly terminating primer extension - This invention relates to the field of nucleic acid chemistry, more specifically to the field of compositions of matter that comprise triphosphates of modified 2′-deoxynucleosides and oligonucleotides that are formed when these are appended to the 3′-end of a primer, wherein said modifications comprise NH | 11-10-2011 |
| 20110281304 | PROCESS FOR PLASMID DNA FERMENTATION - Improvements in plasmid DNA production technology are needed to insure the economic feasibility of future DNA vaccines and DNA therapeutics. General methods are described, by means of which it is possible to dramatically increase plasmid DNA productivity in a fermentor. These processes feature fed-batch fermentation strategies, combined with novel growth and induction phase temperature shifts. | 11-17-2011 |
| 20120070861 | Human Lambda Light Chain Mice - Genetically modified mice are provided that express human λ variable (hVλ) sequences, including mice that express hVλ sequences from an endogenous mouse λ light chain locus, mice that express hVλ sequences from an endogenous mouse κ light chain locus, and mice that express hVλ sequences from a transgene or an episome wherein the hVλ sequence is linked to a mouse constant sequence. Mice are provided that are a source of somatically mutated human λ variable sequences useful for making antigen-binding proteins. Compositions and methods for making antigen-binding proteins that comprise human λ variable sequences, including human antibodies, are provided. | 03-22-2012 |
| 20120115188 | CHIMERIC DNA POLYMERASES - The present invention provides, among other things, chimeric DNA polymerases containing heterologous domains having sequences derived from at least two DNA polymerases that have at least one distinct functional characteristics (e.g., elongation rate, processivity, error rate or fidelity, salt tolerance or resistance) and methods of making and using the same. In some embodiments, the present invention can combine desired functional characteristics (e.g., high processivity; high elongation rate; thermostability; resistance to salt, PCR additives (e.g., PCR enhancers) and other impurities; and high fidelity) of different DNA polymerases in a chimeric polymerase. | 05-10-2012 |
| 20120156728 | CLONAL AMPLIFICATION OF NUCLEIC ACID ON SOLID SURFACE WITH TEMPLATE WALKING - Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided. | 06-21-2012 |
| 20120164689 | METHOD FOR ISOLATING SPECIFIC GENOMIC REGIONS - Provided is a method for specifically isolating any genomic region while maintaining interaction of the genomic region with its interacting molecule(s). According to the method comprising the following steps 1 to 5: | 06-28-2012 |
| 20120190073 | PROCESS FOR LARGE SCALE PRODUCTION OF PLASMID DNA BY E. COLI FERMENTATION - The present invention relates generally to a method for increasing the yield of plasmid DNA production. The method includes the steps of selecting a highly productive clonal subtype of a strain of | 07-26-2012 |
| 20120214206 | VECTORS FOR MOLECULE DELIVERY TO CD11b EXPRESSING CELLS - The invention relates to a novel use of a | 08-23-2012 |
| 20120231508 | NOVEL MULTIPLEX BARCODED PAIRED-END DITAG (mbPED) SEQUENCING APPROACH AND ITS APPLICATION IN FUSION GENE IDENTIFICATION - A method of generating a barcoded Paired-End Ditag (bPED) nucleic acid fragment is disclosed. The method comprises: a) performing a first ligation by ligating a half-adaptor with one or two 3′-overhanging ends to a target nucleic acid to obtain a nucleic acid fragment with two ends each attached to one of the half-adaptor, the half adaptor comprising a half-barcode and a restriction enzyme (RE) recognition site; b) performing a second ligation by ligating two of the half-adaptor at the two ends of the nucleic acid fragment to form a circularized nucleic acid construct, wherein the circularized nucleic acid construct comprises a full-size barcoded adaptor; and c) digesting the circularized nucleic acid construct with a RE that cleaves at a defined distance from the RE recognition site, and thereby generating the bPED nucleic acid fragment. | 09-13-2012 |
| 20120270271 | Toxin-Immunity System - The present invention provides host cells whose survivability can be conditionally controlled, and vectors that can be used for preparing such host cells and for selectable cloning. | 10-25-2012 |
| 20130122549 | RECOMBINANT PHAGE AND METHODS - This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making a plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided. | 05-16-2013 |
| 20130149745 | METHOD FOR PRODUCING CHEMICALS BY CONTINUOUS FERMENTATION - A method produces a chemical by fermentation including: filtering a liquid containing a feedstock, the chemical, and bacterial, microbial or cultured cells through a membrane to recover the chemical from the filtrate; retaining or refluxing unfiltered liquid in the liquid; and adding the feedstock to the liquid, wherein the membrane is a porous hollow-fiber membrane including a polyvinylidene fluoride resin, the porous hollow-fiber membrane having an average pore size of 0.001 μm to 10.0 μm, pure water permeability coefficient at 50 kPa at 25° C. of 0.5 m | 06-13-2013 |
| 20130266988 | METHOD FOR GENE AMPLIFICATION - The present invention provides a double-stranded DNA constructed specifically for high speed gene amplification, a method for gene amplification and a method for synthesizing protein. The gene amplification system of the present invention used a site-specific recombinase such as Cre-lox system and target sequence thereof to efficiently induce a type of replication referred to as a double rolling-circle replication (DRCR). Amplification unit, whose structure is shown in FIG. | 10-10-2013 |
| 20130323791 | RESTRICTED IMMUNOGLOBULIN HEAVY CHAIN MICE - Mice having a restricted immunoglobulin heavy chain locus are provided, wherein the locus is characterized by a single polymorphic human V | 12-05-2013 |
| 20130330774 | TARGETED ALTERATION OF DNA WITH OLIGONUCLEOTIDES - The current invention relates to a method for targeted alteration of acceptor DNA, for example duplex acceptor DNA. The method comprises use of at least two oligonucleotides, each oligonucleotide having at least one mismatch relative to the targeted (duplex) acceptor DNA. The mismatch of the first oligonucleotide is directed to a nucleotide at a position in the first strand of the duplex and the mismatch of the second oligonucleotide is directed to the nucleotide in the second strand that occupies the complementary position in the duplex acceptor DNA (e.g. forms a base-pair with the nucleotide in the first strand). These mismatches are located at specific positions within said oligonucleotides. Also provided is a kit that comprises instructions for performing the method according to the inventions, and in a preferred embodiment, comprises oligonucleotides suitable for use in the method. | 12-12-2013 |
| 20140045219 | Method for Producing Substance Utilizing Microorganism - In a method for producing a target substance utilizing a microorganism comprising culturing the microorganism in a medium to produce and accumulate the target substance in the medium and collecting the target substance, microorganism is employed, which is a mutant strain or a genetic recombinant strain constructed from a parent strain of the microorganism having a respiratory chain pathway of high energy efficiency and a respiratory chain pathway of low energy efficiency as respiratory chain pathways, and having either one or both of the following characteristics: (A) the respiratory chain pathway of high energy efficiency is enhanced, (B) the respiratory chain pathway of low energy efficiency is deficient. | 02-13-2014 |
| 20140154747 | METHODS AND DEVICES FOR PRODUCING BIOMOLECULES - A scalable process and device for producing a bio molecule, in particular pharmaceutical grade plasmid DNA is described. The process includes the steps of alkaline lysis, neutralization and clarification and can be further extended. For separating the lysate and the precipitate an improved floatation method is disclosed. This method is based on attachment of CO | 06-05-2014 |
| 20140193856 | CONTROL OF GENE EXPRESSION - The present invention relates generally to a method of modifying gene expression and to synthetic genes for modifying endogenous gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the present invention utilizes recombinant DNA technology to post-transcriptionally modify or modulate the expression of a target gene in a cell, tissue organ or whole organism, thereby producing novel phenotypes. Novel synthetic genes and genetic constructs which are capable of repressing delaying or otherwise reducing the expression of an endogenous gene or target gene in an organism when introduced thereto are also provided. | 07-10-2014 |
| 20140212928 | METHODS AND COMPOSITIONS FOR TARGETED SINGLE-STRANDED CLEAVAGE AND TARGETED INTEGRATION - Disclosed herein are methods and compositions for generating a single-stranded break in a target sequence, which facilitates targeted integration of one or more exogenous sequences. | 07-31-2014 |
| 20140234908 | USE OF NON-SUBTYPE B GAG PROTEINS FOR LENTIVIRAL PACKAGING - The invention encompasses a lentiviral packaging vector comprising a non-subtype B gag-pol sequence, particularly a subtype D gag-pol sequence. The invention further encompasses methods for making and using these vectors. The invention further encompasses lentiviral vector particles comprising HIV-1 non-subtype B Gag and/or Pol proteins. | 08-21-2014 |
| 20140335568 | NUCLEIC ACID ASSEMBLY, VECTOR, CELL, METHODS AND KIT THEREOF - The present disclosure relates to a nucleic acid assembly (NAA), comprising sensor domain and handle domain; an assembly interfaceable motif (AIM) sequence optionally along with intracellular targeting motif (ITM) sequence; and an AIM-NAA complex. It also relates to a vector comprising assembly interfaceable motif sequence optionally along with intracellular targeting motif sequence and a cell comprising the vector. Further, the instant disclosure also provides a method to obtain the nucleic acid assembly, method of intracellular targeting and kit thereof. | 11-13-2014 |
| 20150118713 | METHOD FOR CONSTRUCTING FUNCTIONAL NUCLEIC ACID MOLECULE, AND NUCLEIC ACID COMBINATION TO BE USED IN SAID METHOD - A method for constructing a functional nucleic acid molecule comprising 1 or 2 nucleic acid strands, wherein 2 or more fragments having at corresponding ends a functional group pair that can mutually couple through a chemical reaction are introduced into a cell, and a functional nucleic acid molecule comprising 1 or 2 nucleic acid strands is formed by ligating mutually the fragments through a reaction between the functional groups in the cell. | 04-30-2015 |
| 20150118714 | VECTORS FOR TRANSGENE EXPRESSION - The present invention relates to a vector system involving replacement of a Woodchuck Hepatitis Virus Post-Transcriptional Regulatory Element (WPRE) sequence with an unrelated short spacer sequence for efficient expression of nucleotides of interest in a retroviral vector system and methods of delivering and expressing nucleotides of interest to target cells. | 04-30-2015 |
| 20150299728 | TC-83-DERIVED ALPHAVIRUS VECTORS, PARTICLES AND METHODS - The present disclosure provides TC-83 VEE-derived replicons, alphaviral replicon particles and immunogenic compositions containing TC-83 alphaviral replicon particles which direct the expression of at least one antigen when introduced into a suitable host cell. The TC-83 VEE-derived ARPs described herein are improved in that they are subject to a lower vector-specific immune response than prior art ARPs. | 10-22-2015 |
| 20150307826 | Method and Microfluidic System for Processing Organic Cells and Manufacturing Method for Producing a Microfluidic System for Processing Organic Cells - A method for processing organic cells includes providing a microfluidic system having a chamber comprising a stationary phase in the form of a plurality of microparticles. The method further includes letting a plurality of organic cells in a mobile phase into the chamber across a first opening of the microfluidic system. The method further includes accumulating the organic cells in a tapered section of the chamber that is upstream of a filter of the microfluidic system that is impermeable to the microparticles. The method further includes flushing a lysis agent into the chamber to resuspend the microparticles and the organic cells in the chamber for a disruption of the organic cells. | 10-29-2015 |
| 20150307911 | METHODS AND DEVICES FOR PRODUCING BIOMOLECULES - A scalable process and device for producing a bio molecule, in particular pharmaceutical grade plasmid DNA is described. The process includes the steps of alkaline lysis, neutralization and clarification and can be further extended. For separating the lysate and the precipitate an improved floatation method is disclosed. This method is based on attachment of CO | 10-29-2015 |
| 20150329874 | VACCINE PREPARED UTILIZING HUMAN PARAINFLUENZA VIRUS TYPE 2 VECTOR - Disclosed are: a virus vector in which a gene encoding an antigenic polypeptide is integrated in human parainfluenza virus type 2 gene, wherein the antigenic polypeptide is expressed in the form of a fusion protein with a viral structural protein; and a method for producing the same. The virus vector of the present invention contains a quantitatively large amount of the antigenic peptide on the virus particle and can efficiently deliver the antigenic polypeptide to a target cell. | 11-19-2015 |
| 20150329891 | ANALYSIS OF NUCLEIC ACIDS ASSOCIATED WITH SINGLE CELLS USING NUCLEIC ACID BARCODES - Provided herein are methods and compositions for analyzing nucleic acids associated with single cells using nucleic acid barcodes. According to some embodiments, a method for producing one or more polynucleotides of interest comprises: obtaining a plurality of RNAs associated with one or more samples, wherein the samples are obtained from one or more subjects, each RNA is associated with a single sample, and the RNAs associated with each sample are present in a separate reaction volume; adding an adapter molecule to the RNAs associated with each sample, wherein the adapter molecule is generated using an enzymatic reaction and comprises a universal priming sequence, a barcode sequence, and a binding site; and incorporating the barcode sequence into one or more polynucleotides associated with each sample, thereby producing the one or more polynucleotides of interest. | 11-19-2015 |
| 20190144482 | Reversibly Blocked Nucleoside Analogues And Their Use | 05-16-2019 |
| 20190144490 | COMPOSITIONS AND METHODS FOR SYNTHESIZING 5'-CAPPED RNAS | 05-16-2019 |
