| Entries |
| Document | Title | Date |
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| 20090221040 | ANTIBODIES ACTIVE AGAINST A FUSION POLYPEPTIDE COMPRISING A HISTIDINE PORTION - The present invention relates to an antibody active against a fusion polypeptide comprising a histidine portion, a process for the preparation thereof and its use. | 09-03-2009 |
| 20090317869 | METHODS AND COMPOSITIONS FOR MAKING ANTIBODIES AND ANTIBODY DERIVATIVES WITH REDUCED CORE FUCOSYLATION - The invention provides methods and compositions for preparing antibodies and antibody derivatives with reduced core fucosylation. | 12-24-2009 |
| 20130323790 | HUMAN LAMBDA LIGHT CHAIN MICE - Genetically modified mice are provided that express human λ variable (hVλ) sequences, including mice that express hVλ sequences from an endogenous mouse λ light chain locus, mice that express hVλ sequences from an endogenous mouse κ light chain locus, and mice that express hVλ sequences from a transgene or an episome wherein the hVλ sequence is linked to a mouse constant sequence. Mice are provided that are a source of somatically mutated human λ variable sequences useful for making antigen-binding proteins. Compositions and methods for making antigen-binding proteins that comprise human λ variable sequences, including human antibodies, are provided. | 12-05-2013 |
| 20140087423 | Method for Controlling the Main Complex N-Glycan Structures and the Acidic Variants and Variability in Bioprocesses Producing Recombinant Proteins - The present invention relates to a method of controlling quality and quantity of posttranslational modification of a recombinantly produced polypeptide/protein (glycoprotein), wherein the posttranslational modification affects the glycosylation profile and/or the acidic variants profile, as manifested in CEX profiles, wherein the polypeptide/protein (glycoprotein) production is in eukaryotic host cells, the method comprising the following steps: a) cultivating the eukaryotic cells in a suitable medium under conditions which allow the expression of the polypeptide/protein, wherein the content of the dissolved CO | 03-27-2014 |
| 20140193855 | METHODS AND COMPOSITIONS FOR DETERMINING THE PURITY OF CHEMICALLY SYNTHESIZED NUCLEIC ACIDS - This application describes an antibody that specifically binds to a synthetic oligomer (e.g., an oligonucleotide or oligopeptide) having a organic protecting group covalently bound thereto, which antibody does not bind to that synthetic oligomer when the organic protecting group is not covalently bound thereto. Methods of making and using such antibodies are also disclosed, along with cells for making such antibodies and articles carrying immobilized oligomers that can be used in assay procedures with such antibodies. | 07-10-2014 |
| 20080199883 | Schizophrenia Related Gene and Protein - The invention relates to polynucleotides of the PAPAP gene, polypeptides encoded by the PAPAP gene, and antibodies directed specifically against such polypeptides. The invention also concerns methods for the treatment or diagnosis of schizophrenia, bipolar disorder or related CNS disorder. The invention also concerns the interaction of PAPAP with schizophrenia candidate gene g34872. | 08-21-2008 |
| 20080199884 | Method For Measuring Protozoan Oocyst and Detecting Reagent - The present invention provides a method for measuring oocyst of protozoa, such as | 08-21-2008 |
| 20080206784 | Target Substance-Capturing Body, Device for Capturing Target Substance, and Instrument, Kit and Method for Detecting Target Substance by Use of Them - It is intended to provide a target substance-capturing body comprising: a base consisting of a soluble protein; and two or more functional domains capable of binding to different target substances. | 08-28-2008 |
| 20080206785 | ASSAYS FOR SENSORY MODULATORS USING A SENSORY CELL SPECIFIC G-PROTEIN ALPHA SUBUNIT - The invention identifies nucleic acid and amino acid sequences of a sensory cell specific G-protein alpha subunit that are specifically expressed in sensory cells, e.g., taste cells, antibodies to such G-protein alpha subunits, methods of detecting such nucleic acids and subunits, and methods of screening for modulators of a sensory cell specific G-protein alpha subunit. | 08-28-2008 |
| 20080206786 | Trace amine receptor 1 of the african green monkey - A trace amine receptor 1 has been isolated from the genome of | 08-28-2008 |
| 20080213798 | NOVEL C. ELEGANS P21-ACTIVATED KINASE (PAK) GENE AND ASSOCIATED LOSS-OF-FUNCTION PHENOTYPES THAT FACILITATE SCREENING FOR SMALL MOLECULE MODULATORS OF PAK ACTIVITY IN THE NEMATODE, CAENORHABDITIS ELEGANS - The invention refers to a novel | 09-04-2008 |
| 20080213799 | Method for Detection of Substance Bound to Nuclear Receptor - Provided is a detection method including exposing, to contact with a surface to which a cofactor has been bound, a nuclear receptor protein serving as a counterpart of the cofactor and a test sample; and detecting a substance which is contained in the test sample and which binds to the nuclear receptor, on the basis of a change in degree of binding between the nuclear receptor protein and the cofactor. The detection method is means for detecting a living-body-related substance, which means employs a nuclear receptor-cofactor system, exhibits detection high sensitivity, provides a convenient detection process, and realizes efficient establishment of a detection system. | 09-04-2008 |
| 20080220444 | Bone Marrow Proliferation Assay - Disclosed herein are assay methods for assessing the myelotoxicity of a pharmacologic agent or a putative pharmacologic agent. Also, disclosed are kits and mixtures of cytokines and growth factors useful in the assay methods disclosed herein. | 09-11-2008 |
| 20080220445 | Method of Screening Transmembrane Enzyme Inhibitory Substance - The present invention provides a screening method for a compound inhibiting a transmembrane enzyme activity by binding to a transmembrane region of the enzyme, characterized by using (a) a protein having a part or all of an amino acid sequence of the enzyme, comprising a region comprising an active center and a part or all of a transmembrane region of the transmembrane enzyme, and optionally (b) a protein having a part of an amino acid sequence of the transmembrane enzyme, comprising the region comprising the active center and lacking the above-mentioned part or all of the transmembrane region, and measuring the binding of a test substance to each protein and the enzyme activity of each protein, as well as a kit for screening comprising the above-mentioned protein of (a) and (b). Also, the present invention provides a β-secretase selective inhibitor comprising a β-secretase inhibiting substance binding to a transmembrane region of the enzyme, and particularly an inhibitor for prophylaxis and/or treatment of Alzheimer's disease, Down syndrome or Age-Associated Memory Impairment. | 09-11-2008 |
| 20080220446 | Method for Detecting and/or Removing Protein and/or Peptide Comprising a Cross-Beta Structure From an Aqueous Solution Comprising a Protein - The invention relates to the field of aqueous solutions comprising a protein. More specifically, the invention relates to the detection and/or removal of conformationally altered proteins and/or peptides comprising a cross-β structure from an aqueous solution comprising a protein. The invention provides methods for detecting and/or removing proteins and/or peptides comprising a cross-β structure from an aqueous solution comprising a protein, said method comprising contacting said aqueous solution comprising a protein with at least one cross-β structure-binding compound resulting in a bound protein or peptide with cross-β structure. The invention further provides a aqueous solution comprising a protein obtainable by a method of the invention, and a kit for carrying out the methods of the invention. | 09-11-2008 |
| 20080227114 | COMPOSITIONS AND METHODS FOR MODULATING C-REL-DEPENDENT CYTOKINE PRODUCTION - The present invention is directed to compositions and methods for modulating c-Rel-dependent cytokine production without materially altering the level of expression of NFκB and/or the amount of IκB. The present invention is also directed to screening for modulators of c-Rel activity as determined by assaying for altered subcellular localization of c-Rel but where the level of expression of NFκB and/or the amount of IκB is materially unaltered. | 09-18-2008 |
| 20080241858 | RAPID MICROBIAL DETECTION AND ANTIMICROBIAL SUSCEPTIBIILITY TESTING - A method for the detection of microorganisms in a sample comprising contacting said sample with a biosensor concentration module, allowing microorganisms to grow for a first period of time and detecting growth of discrete microorganisms as an indication of the presence of said microorganisms. | 10-02-2008 |
| 20080241859 | AGLYCO PRODUCTS AND METHODS OF USE - Glycoconjugates, therapeutic compositions containing the glycoconjugates and therapeutic methods of using the glycoconjugates are disclosed. In particular, peptide constituents of aglyco 10B, which are immunogenic epitopes responsible for recognition of antigens by the immune system are provided. These glycoconjugates are useful in prevention of influenza virus binding to cells, treatment of schizophrenia and diagnosing chronic viral disease associated with development of cancer. | 10-02-2008 |
| 20080241860 | METHODS AND APPARATUS TO RECOGNIZE NERVE INJURIES - Methods and apparatus are described to recognize nerve injuries, specifically brachial plexus injuries, and their severity, preferably of newborn children. To achieve an inexpensive and quick test, which can be executed without harming the patient, it is proposed to measure the motor neuron loss by measuring a biochemical marker, i.e. NSE or protein S100 in a body fluid. | 10-02-2008 |
| 20080248492 | Membrane Based Assays - Membrane-based assays using surface detector array devices suitable for use with a biosensor are disclosed. The device is formed of a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions. The bilayer-compatible surface regions carry on them, separated by an aqueous film, supported fluid bilayers. The bilayers may contain selected receptors or biomolecules. A bulk aqueous phase covers the bilayers on the substrate surface. Arrays may be engineered to display natural membrane materials in a native fluid bilayer configuration, permitting high-throughput discovery of drugs that target and affect membrane components. The membrane-based assays detect binding events by monitoring binding-induced changes in one or more physical properties of fluid bilayers. | 10-09-2008 |
| 20080261237 | Inositol-Phosphate Derivatives and Method of Detecting Inositol-1-Phosphate - The present invention relates to inositol phosphate derivatives, in which the inositol phosphate is substituted with one or two reactive groups G or one or two conjugated substances or molecules M, said reactive group(s) G or said substance(s) or molecule(s) M being linked to IP1 via a linkage group L, M being chosen from the following group: a tracer, an immunogen, a member of a binding partner pair, a solid support. | 10-23-2008 |
| 20080268465 | Process and Kit for Determining Binding Parameters of Bioaffinity Binding Reactions - A process for determining binding parameters, including dissociation constants and sorption rate constants, of the binding between a biomolecule and a binding partner thereof, both being present in a liquid phase, and comprising a marker-free binding step, characterized by the following steps: (a) attaching either (a | 10-30-2008 |
| 20080268466 | Method and Device for Trichomonas Detection - A method and kit for detecting | 10-30-2008 |
| 20080268467 | Methods and compositions for modulating telomerase reverse transcriptase (TERT) expression - Methods and compositions are provided for modulating, e.g., increasing or decreasing, the expression of telomerase reverse transcriptase (TERT). In the subject methods, the binding interaction of the TERT Site C repressor site with a Site C repressor protein complex made up of one or more proteins is modulated to achieve the desired change in TERT expression. A feature of the subject invention is that the target Site C repressor protein complex includes a MRG15 protein. The subject methods and compositions find use in a variety of different applications, including the immortalization of cells, the production of reagents for use in life science research, therapeutic applications; therapeutic agent screening applications; and the like. | 10-30-2008 |
| 20080268468 | Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells - The present disclosure relates to a method for metabolic oligosaccharide engineering that incorporates derivatized alkyne-bearing sugar analogs as “tags” into cellular glycoconjugates. The disclosed method incorporates alkynyl derivatized Fuc and alkynyl derivatized ManNAc sugars into a cellular glycoconjugate. A chemical probe comprising an azide group and a visual probe or a fluorogenic probe is used to label the alkyne-derivatized sugar-tagged glycoconjugate. In one aspect, the chemical probe binds covalently to the alkynyl group by Cu(I)-catalyzed [3+2] azide-alkyne cycloaddition and is visualized at the cell surface, intracellularly, or in a cellular extract. The labeled glycoconjugate is capable of detection by flow cytometry, SDS-PAGE, Western blot, ELISA or confocal microscopy, and mass spectrometry. | 10-30-2008 |
| 20080268469 | Systems and Methods for Analyzing a Particulate - Systems and methods are provided for analyzing particulates. A liquid having a plurality of particulates substantially linearly ordered in a streamline can be externally controlled to provide flow in first and second directions, where, generally, the first direction is opposite to the second direction. A target particulate can be measured from the plurality of particulates at or near a measurement area while the liquid flows in the first flow direction. The flow direction can be reversed and measured at the measurement area while flowing in the second direction. The particulates substantially retain the same linear order during at least one cycle, a cycle being defined by movement in the first direction followed by movement in the second direction. | 10-30-2008 |
| 20080286805 | Recombinant plasmodium falciparum merozoite surface proteins 4 and 5 and their use - Accordingly, the invention provides constructs in which the nucleic acids encoding | 11-20-2008 |
| 20080286806 | Ligand for G-protein coupled receptor GPR43 and uses thereof - The present invention is related to the G-protein coupled orphan receptor GPR | 11-20-2008 |
| 20080286807 | Methods and Reagents for Elimination or Reduction of False Positives in the Analysis of a Sample - Methods, apparatuses, and systems for eliminating or reducing false positives in assays are provided. More specifically, there is provided a method to absorb a false positive signal with an absorber in favor of detecting a true positive signal. Embodiments of the present invention include but are not limited to systems and methods for detecting antibodies with greater specificity than available assays and also for detecting a greater variety of antibodies. | 11-20-2008 |
| 20080293076 | Method to improve barrier function of cell-cell junctions - Described are methods of increasing the barrier function of a cell-cell junction, comprising activating a PDZ-GEF2 protein, wherein the PDZ-GEF2 protein comprises an amino acid sequence encoded by a gene corresponding to the RapGEF6 gene. Further described are methods for selecting an activator of PDZ-GEF2, comprising contacting a plurality of candidate compounds to at least two cells of a first cell type and at least two cells of a second cell type, both cell types capable of forming cell-cell junctions, measuring the maturation of cell-cell junctions before and after contacting the two cell types with the plurality of candidate compounds, and selecting a compound from the plurality of candidate compounds that is more capable of increasing the integrity of a cell-cell junction between at least two cells of the first cell type, as compared to its capability of increasing the integrity of a cell-cell junction between cells of the second type, wherein in the second cell type, the amount and/or activity of PDZ-GEF2 is decreased, compared to the first cell type. | 11-27-2008 |
| 20080293077 | Novel genes encoding proteins having prognostic, diagnostic, preventive, therapeutic and other uses - The invention relates to the discovery and characterization of several genes and the polypeptides they encode: thymotaxin (Tango-45), Tango-63d, Tango-63e, Tango-67, and huchordin (Tango-66). Thymotaxin is a new member of the C-C family of chemokines. Tango-63d and Tango-63e are two novel polypeptides within the tumor necrosis factor (TNF) receptor superfamily. Tango-67 is related to a number of growth factors, particularly members of the connective tissue growth factor family. Huchordin is related to chordin, a known protein that is involved in the induction of twinned axes, can completely rescue axial development in ventralized embryos, is a potent dorsalizing factor, and plays a crucial role in regulating cell-cell interactions in the organizing centers of head, trunk, and tail development. The invention encompasses nucleic acid molecules encoding nucleic acids and polypeptides of the invention, or mutant forms thereof that encode dysfunctional receptor polypeptides, vectors containing these nucleic acid molecules, cells harboring recombinant DNA molecules encoding nucleic acids or polypeptides of the invention, or mutant forms thereof, host fusion proteins that include functional or dysfunctional polypeptides of the invention, transgenic animals that express nucleic acids or polypeptides of the invention, screening methods and therapeutic methods employing the nucleic acid molecules and polypeptides described above, substantially purified nucleic acids and polypeptides of the invention, and therapeutic compositions containing these nucleic acid molecules and polypeptides. | 11-27-2008 |
| 20080311590 | Portable Materials and Methods for Ultrasensitive Detection of Pathogen and Bioparticles - The present invention provides systems for ultrasensitive detection of pathogens and bioparticles. One embodiment of the system comprises an optical detection scheme that allows for the detection of the fluorescence signal of bacteria or other bioparticles in less than about 20 minutes. A microflow channel allows for an assay probing volume of as little as a few picoliters. In one embodiment, the system uses RuBpy dye-doped silica nanoparticles bioconjugated with specific monoclonal antibodies of the target bioparticles. The system allows for the rapid and highly sensitive and specific detection of bacteria or other bioparticles without the need for amplification or enrichment of the sample. | 12-18-2008 |
| 20090004674 | ACPL-related assays - The invention is directed to purified and isolated novel ACPL polypeptides, the nucleic acids encoding such polypeptides, processes for production of recombinant forms of such polypeptides, antibodies generated against these polypeptides, fragmented peptides derived from these polypeptides, and the uses of the above. | 01-01-2009 |
| 20090011430 | ELECTRICAL DETECTION USING CONFINED FLUIDS - A device having: a laminar flow channel for liquids; two or more electrodes; a confining fluid inlet; a sample inlet; and a meter for measuring the impedance of any fluid between the electrodes. The device may have one or more specific binding sites, or it may have sheathing and unsheathing fluid transporting structures. A method of: providing the device; flowing a confining fluid and a conductive liquid that may contain cells or particles through the channel as described herein; and measuring the impedance between the electrodes. | 01-08-2009 |
| 20090035788 | Genetically encoded bioindicators of calcium-ions - The present invention relates to novel types of cellular calcium probes that are based on Troponin C and two chromophors suitable for FRET (fluorescence resonance energy transfer). The Troponin C-based calcium sensors of the invention function in diverse subcellular environments, for example even when tethered to a cellular membrane. The invention further provides nucleic acid constructs encoding the calcium probes of the invention, expression constructs, host cells and transgenic animals. Furthermore, methods for the detection of changes of local calcium concentrations and for detecting the binding of a small molecule to fragments of Troponin C are provided. | 02-05-2009 |
| 20090042215 | Cell Permeability Assay in a Living Array of Multiple Cell Types and Multiple Layers of a Porous Substrate - The present invention provides a device for determining drug efficacy on target cells comprising (1) a porous support (B) having first and second surfaces and at least one area with a plurality of through-going channels, wherein said area comprises on said first surface at least one region, which can be contacted with target cells, and said area comprises on said second surface at least one region comprising a layer of barrier cells presenting a significant barrier adhered to said second surface, wherein said region on the first surface is located opposite to said region on the second surface of the porous support (B), and wherein said layer of barrier cells provides a barrier for the passage of drugs, and (2) a support (A) comprising drugs, which can be contacted with the barrier cells of the porous support (B). | 02-12-2009 |
| 20090042216 | Method for purifying proteins and/or biomolecule or protein complexes - The present invention relates to a method for detecting and/or unifying proteins and/or biomolecule or protein complexes, as well as fusion proteins, nucleic acids, vectors and cells suitable for this method. | 02-12-2009 |
| 20090047687 | Indane Acetic Acid Derivatives and Their Use as Pharmaceutical Agents, Intermediates, and Method of Preparation - This invention relates to novel indane acetic acid derivatives which are useful in the treatment of diseases such as diabetes, obesity, hyperlipidemia, and atherosclerotic diseases. The invention also relates to intermediates useful in preparation of indane acetic derivatives and to methods of preparation. | 02-19-2009 |
| 20090068681 | METHODS AND COMPOSITIONS FOR RISK STRATIFICATION - The present invention provides an approach for the simultaneous determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of potentiators of cellular activation allows for characterization of such pathways and cell populations. | 03-12-2009 |
| 20090075294 | Eukaryotic mechanosensory transduction channel - The present invention provides, for the first time, nucleic acids encoding a eukaryotic mechanosensory transduction channel (MSC) protein. The proteins encoded by these nucleic acids form channels that can directly detect mechanical stimuli and convert them into electrical signals. These nucleic acids and the proteins they encode can be used as probes for sensory cells in animals, and can be used to diagnose and treat any of a number of human conditions involving inherited, casual, or environmentally-induced loss of mechanosensory transduction activity. | 03-19-2009 |
| 20090075295 | PORPHYRINIC COMPOUNDS FOR USE IN FLOW CYTOMETRY - The present invention provides a method of detecting (e.g., by flow cytometry) a target compound, cell or particle, wherein the target is labelled with a detectable luminescent compound. The method comprises utilizing as the detectable luminescent compound a compound comprising a porphyrinic macrocycle such as a porphyrin, chlorin, bacteriochlorin, or isobacteriochlorin. In particular embodiments, the detectable luminescent compound comprises a compound of the formula A-A′-Z-B′—B, wherein: A is a targeting group or member of a specific binding pair that specifically binds the detectable luminescent compound to the target compound, cell or particle; A′ is a linker group or covalent bond; B′ is a linker group or covalent bond; B is a water-soluble group; and Z is the porphyrinic macrocycle. | 03-19-2009 |
| 20090075296 | METHODS AND COMPOSITIONS FOR ASSAYING REGULATORY T CELLS - Methods and compositions for determining whether a T cell is a regulatory T cell and for determining whether a population of cells includes at least one regulatory T cell or a cell with the potential to become a regulatory T cell. The invention includes methods and compositions for detecting TGF-β on the surface of a cell. The invention also provides methods and compositions for evaluating the suppressive activity of a regulatory T cell based on the presence and/or the amount of membrane-bound TGF-β. | 03-19-2009 |
| 20090075297 | MAMMALIAN GALANIN RECEPTORS - The present invention provides isolated mammalian GalR3 receptors, isolated or recombinant nucleic acids and recombinant vectors encoding the same, host cells comprising the nucleic acids and vectors, and methods for making the receptors using the host cells. This invention further provides antibodies and antigen binding fragments thereof which specifically bind to the receptors and are useful for treating medical conditions caused or mediated by galanin. Also provided are screening methods for identifying specific agonists and antagonists of the mammalian GalR3 receptors. | 03-19-2009 |
| 20090081698 | High throughput cell-based assays, methods of use and kits - In the present invention cells are placed in a multiwell plate and grown. When the assay is to be performed, one uses gravity to wash away any unbound ligands rather than vacuum or centrifugation. The cells are then examined to detect the bound ligand. To perform the washing step(s) the plate is placed into a carrier pate having open wells in register with the wells of the filter plate or one may use a wicking device or an underdrain attached to the bottom of the filter plate. Sufficient wash liquid is added to allow for filtration by the effect of gravity to occur. Cells are retained within the wells at a rate of 4 times that of other rapid methods. | 03-26-2009 |
| 20090081699 | METHODS AND COMPOSITIONS FOR RISK STRATIFICATION - The present invention provides an approach for the simultaneous determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of potentiators of cellular activation allows for characterization of such pathways and cell populations. | 03-26-2009 |
| 20090081700 | Methods of use of the TACI/TACI-L interaction - The invention discloses a novel interaction between a TNF receptor (TACI) and its interacting ligand (TACI-L). Also disclosed are methods of screening candidate molecules to determine potential antagonists and agonists of the TACI/TACI-L interaction. The use of the antagonists and agonists as therapeutics to treat autoimmune diseases, inflammation, and to inhibit graft vs. host rejections is further disclosed. | 03-26-2009 |
| 20090081701 | METHOD OF SELECTIVE PROTEIN ENRICHMENT AND ASSOCIATED APPLICATIONS - The present invention provides methods of selective enrichment of ligands present in a biological sample. One or a plurality of receptor carriers are used to capture ligands capable of binding to receptors immobilized on the surface of the receptor carriers. Receptor carriers bound with the ligands are separated from the remaining sample and the ligands are then eluted with a ligand elution solution to result in an enriched ligand sample. The enriched ligand sample may be used for further isolation of one or more ligands of interest, or for ligand profiling using 2-D gel electrophoresis coupled with mass spectrometry, for example. Such ligand profiling may have a number of applications, such as disease diagnosis, pathogen detection and drug screening. | 03-26-2009 |
| 20090087863 | Method For Detecting Polypeptide Toxic to Diabrotica Insects - Disclosed is a novel Lepidopteran- and Coleopteran-active δ-endotoxin polypeptide, and compositions comprising the polypeptide, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that comprise nucleic acid segments encoding the polypeptide. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising the novel sequences of the invention, as well as methods for controlling an insect population, such as the Western Corn Rootworm and Colorado potato beetle, and for conferring to a plant population resistance to the target insect species. | 04-02-2009 |
| 20090093000 | Glycoprotein and apolipoprotein biopolymer markers predictive of Alzheimers disease - The instant invention involves the use of a combination of preparatory steps in conjunction with mass spectroscopy and time-of-flight detection procedures to maximize the diversity of biopolymers which are verifiable within a particular sample. The cohort of biopolymers verified within such a sample is then viewed with reference to their ability to evidence at least one particular disease state; thereby enabling a diagnostician to gain the ability to characterize either the presence or absence of said at least one disease state relative to recognition of the presence and/or the absence of said biopolymer, predict disease risk assessment, and develop therapeutic avenues against said disease. | 04-09-2009 |
| 20090093001 | HIGH CONTENT SCREENING - A high content screening (HCS) assay for rapidly screening one or more compounds to determine functional response or pharmacological properties thereof, comprising: i) priming a cell or cell material with a sensor for a biological response; ii) contacting the compound(s) to be tested with the primed cell or cell material or contacting a cell or cell material which has been contacted with the compound(s) with the primed cell or cell material; iii) simultaneously or subsequently contacting with a fluorescent agonist or a fluorescent neutral antagonist wherein the binding of the fluorescent agonist or antagonist and its associated biological response are detected or monitored in the same cell and are distinct allowing separate readout. | 04-09-2009 |
| 20090098573 | Nucleic acids and proteins of insect Or83b odorant receptor genes and uses thereof - The present invention relates to insect odorant receptor genes and methods for identifying odorant receptor genes. The invention provides nucleotide sequences of insect odorant receptor genes Or83b, amino acid sequences of their encoded proteins (including peptides or polypeptides), and related products and methods. The nucleic acids of the invention may be operatively linked to promoter sequences and transformed into host cells. Methods of production of an Or83b odorant receptor protein (e.g., by recombinant means), and derivatives and analogs thereof, are provided. Antibodies to an Or83b odorant receptor protein, and derivatives and analogs thereof, are provided. Methods for identifying molecules that bind or modulate the activity of these Or83b odorant receptor genes are provided. Molecules found to bind or modulate the activity of Or83b genes may be formulated into pest control agents by providing a carrier. In a preferred embodiment, molecules that bind or modulate the activity of an Or83b gene from one species but not others is desired. Methods to modify the insect behavior by modifying an insect Or83b odorant are also provided. | 04-16-2009 |
| 20090098574 | FUNCTIONALIZATION OF GOLD NANOPARTICLES WITH ORIENTED PROTEINS, APPLICATION TO THE HIGH-DENSITY LABELLING OF CELL MEMBRANES - The present invention relates to nanoparticles the surface of which is modified by deposition of proteins. The invention further relates to a method for producing said nanoparticles and to their use in biological research and in the biomedical field (for example labelling and diagnosis). | 04-16-2009 |
| 20090111123 | COMPOSITIONS FOR DETECTING ANTIBODIES TO BABESIA MICROTI AND METHODS OF USES THEREOF - The present invention relates to one or more peptides which may be used to test for the presence of antibodies to | 04-30-2009 |
| 20090117590 | Human calcium sensitive potassium channel beta3 subunit proteins, encoding nucleic acid and uses thereof - The present invention is directed to novel human DNA sequences encoding calcium sensitive potassium channel subunits β2, β3a, β3b, β3c, and β3d, the proteins encoded by the DNA sequences, vectors comprising the DNA sequences, host cells containing the vectors, and methods of identifying inhibitors and agonists of calcium sensitive potassium channels containing human β2, β3a, β3b, β3c, or β3d subunits and inhibitors and agonists of β3 gene transcription. | 05-07-2009 |
| 20090123938 | Mammalian monocyte chemoattractant protein receptors - Novel human chemokine receptors, MCP-1RA and MCP-1RB, and processes for producing them are disclosed. The receptors, which are alternately spliced versions of MCP-1 receptor protein may be used in an assay to identify antagonists of MCP-1 which are therapeutically useful in the treatment of atherosclerosis and other diseases characterized by monocytic infiltrates. | 05-14-2009 |
| 20090123939 | Biologically enhanced electrically-active magnetic nanoparticles for concentration, separation, and detection applications - The disclosure generally relates to a particulate composition formed from a conductive polymer (e.g., conductive polyanilines, polypyrroles, polythiophenes) bound to magnetic nanoparticles (e.g., Fe(II)- and/or Fe(III)-based magnetic metal oxides). The particulate composition can be formed into a biologically enhanced, electrically active magnetic (BEAM) nanoparticle composition by further including a binding pair member (e.g., an antibody) bound to the conductive polymer of the particulate composition. Methods and kits employing the particulate composition and the BEAM nanoparticle composition also are disclosed. | 05-14-2009 |
| 20090123940 | MOLECULES INVOLVED IN SYNAPTOGENESIS AND USES THEREFOR - The present invention is based on the discovery that neuronal pentraxins play a role in the clustering and internalization of AMPA receptors, synaptogenesis, and metabotropic glutamate receptor-mediated long term depression (LTD) of a synapse. Accordingly, there are provided methods of identifying compounds that that modulate mGluR-mediated AMPA receptor internalization and LTD. Further provided are cleavage products of a member of the neuronal pentraxin family, neuronal pentraxin receptor (NPR). Also provided are isolated peptides comprising the Narp association regions 1 and 2 (NAR1 and NAR2, respectively) and the Narp binding motif (NBM) of AMPA receptors. Finally, there are provided antibodies that block binding of neuronal pentraxins to AMPA receptors, in particular, antibodies that bind NAR1, or NAR2, or NBM. | 05-14-2009 |
| 20090130691 | SCREENING PROTEINASE MODULATORS USING A CHIMERIC PROTEIN AND SKI-I PROPROTEIN CONVERTASE SUBSTRATES AND INHIBITORS - A chimeric protein comprising in sequence a signal peptide, a first amino acid tag, a proteinase bait, a second amino acid tag, a transmembrane domain and a cytosolic domain, wherein the cytosolic (CT) domain comprises a sequence able to recycle the protein from the cellular membrane to endosomes. A cell line expressing the chimeric protein and an assay using the cell line. | 05-21-2009 |
| 20090136968 | Novel in vitro methods for studying receptors recognizing volatile compounds - The present invention in particular relates to in vitro methods to identify and/or confirm the binding and/or function of a volatile compound onto a membrane-integrated receptor using volatile-compound-Binding Protein (BP) or compositions thereof. Additionally, the present invention relates to kits comprising receptor proteins recognizing volatile compounds or a candidate receptor for said compound; and; BP, a complex or composition thereof. Said kits may be used to identify and/or confirm the binding and/or function of volatile compounds onto a membrane-integrated receptor. | 05-28-2009 |
| 20090142775 | Methods For Separating Organelles - Methods and kits for separating organelles of a specific type from a mixture of organelles of different types are described. The methods comprise providing a mixture of organelles of different types, adding probes to the mixture to form an organelle-probe complex with a distinct diffusion coefficient, and separating the organelles of the specific type from the mixture of organelles based upon the diffusion coefficient of the complex. The probes used in these methods include an affinity portion which binds selectively to the organelle of the specific type and a tag portion which imparts the distinct diffusion coefficient to the complex. | 06-04-2009 |
| 20090170122 | Human intestinal NPT2B - A novel human sodium phosphate cotransporter expressed on the apical surface of intestinal epithelial cells (huNpt2B) and polypeptides related thereto, as well as nucleic acid compositions encoding the same, are provided. The subject polypeptides and nucleic acid compositions find use in a variety of applications, including research, diagnostic, and therapeutic agent screening applications. Also provided are methods of inhibiting Npt2B activity in a host and methods of treating disease conditions associated with Npt2B activity. | 07-02-2009 |
| 20090170123 | Identification of Novel Protein Targets on the Surface of Stressed Cells - The present invention in the field of biochemistry and medicine is directed to novel methods for identifying molecules, typically proteins, that move to the cell surface when cells are stimulated or stressed can act as receptors even thought they are not transmembrane molecules and normally originate in the cytosol. Such molecules are useful targets for development of agents that can image or treat tumors or other pathologies. Methods to detect or identify such proteins that have translocated to the cell surface when cells are stressed by an angiogenic environment, environmental stresses, the stimulation of cell proliferation and differentiation, or after exposure to certain drugs such as cancer chemotherapeutics, are disclosed. | 07-02-2009 |
| 20090170124 | HYDROGEL THIN FILM FOR USE AS A BIOSENSOR - The present disclosure provides a biosensor capable of producing an indicator response upon detection of the presence of certain metabolites in a biological sample. The biosensor includes a hydrogel that is functionalized with affinity molecules specific to markers for one or more pathogens. The biosensor also includes a detection system adapted to detect the binding the pathogen-specific markers with their corresponding affinity molecules. | 07-02-2009 |
| 20090176250 | Immunoliposome-Nucleic Acid Amplification (ILNAA) Assay - Immunoliposomes and use thereof in highly specific and sensitive nucleic acid amplification assays relying on amplification of specific nucleic acid sequences released from encapsulation within a liposome after a receptor on the liposome couples with a targeted analyte/antigen immobilized on a select surface. The immunoliposome nucleic acid amplification assay permits both quantitative and qualitative analyte detection. | 07-09-2009 |
| 20090191572 | Imaging, diagnosis and treatment of disease - The present invention relates to endothelial cell-specific genes and encoded polypeptides and materials and uses thereof in the imaging, diagnosis and treatment of conditions involving the vascular endothelium. | 07-30-2009 |
| 20090191573 | METHOD AND KIT FOR DETERMINING ANTIGEN CONTENT IN A SAMPLE USING DOUBLE IMMUNOHISTOCHEMICAL DETECTION - A method for determining antigen content in a region of interest of a sample is disclosed. The method includes a sequential immunomarking resulting in two chromogenic signals, which are respectively provided by a labile and a stabile chromogenic product. The method further involves acquisition, analysis and evaluation of digital images from such signals. A kit for determining antigen content in a region of interest of a sample is also described. | 07-30-2009 |
| 20090197282 | METHOD AND SYSTEM FOR SCREENING THE RECEPTOR-LIGAND BINDING IN LIVE CELL - The invention relates to methods and system for detecting ligand binding to membrane receptors and endocytosis. A method for detecting ligand binding to a membrane receptor includes the steps of: incubating the cell with a dye and a ligand; and detecting dye-containing endocytic vesicles in the cell. A system for screening ligand binding to membrane receptors includes an automatic liquid handling device; an x-y stage for positioning a plate; a microscope configured to image endocytic vesicles of a cell; a software for automatic analysis of FM spots (endocytic vesicles); and a control unit configured to control the movement of the x-y stage and the microscope. | 08-06-2009 |
| 20090203040 | Detection of specific nitrated markers - Methods are described for improving the diagnostic possibilities of diseases where oxidative NO-modifications occur, for example inflammatory conditions, cancer, Parkinson's or Alzheimer's disease, and to provide means of monitoring the effects of therapeutical measures taken towards such diseases. The invention enables the detection of disease specific catabolic markers related to oxidative NO-modifications, utilizing an immunoassay comprising antibodies directed against nitrated and non-nitrated epitopes characteristic of a specific protein. | 08-13-2009 |
| 20090203041 | BMP4 INHIBITORS - A fragment of Gremlin having the sequence of amino acids shown in SEQ ID NO:2 or a functional subsequence of the fragment is described. The fragment is capable of altering BMP4 activity. Also provided is a polynucleotide encoding the fragment. Also disclosed is an assay for testing a molecule for Gremlin inhibitory activity. The invention also provides a method of inhibiting BMP4 activity. | 08-13-2009 |
| 20090203042 | NOVEL BT TOXIN RECEPTORS FROM LEPIDOPTERAN INSECTS AND METHODS OF USE - The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins. | 08-13-2009 |
| 20090208975 | Device and methods for detecting a target cell - The present invention relates to devices for detecting intact target cells in a sample comprising a detection zone comprising an immobilized specific binding reagent, capable of forming a complex with a target analyte on a target cell. Once labeled, detection of the label indicates the absence, presence and/or amount of the target cell in a sample. The embodiments further relate to kits comprising the devices, and methods of using the devices to screen for the presence, absence, and/or amount of a target cell in a sample. | 08-20-2009 |
| 20090215079 | Method and Devices for the Detection of Microorganisms and/or the Activity Thereof - In a method for detection of microorganisms and/or their activity with biosensors, on a surface of a substrate over portions thereof at least one ligand for binding a receptor or at least one receptor for a ligand or at least one ligand for binding a receptor and at least one receptor for either the one or a further ligand are immobilized chemically, physically, or biologically, wherein physical or physicochemical changes, caused at receptors by binding of ligands that are emitted by microorganisms in the process of quorum sensing are measured. | 08-27-2009 |
| 20090220988 | METHOD OF REVEALING A BIOLOGICAL PROCESS USING A FRET MEASUREMENT - The present invention relates to a method of revealing a biological process using a FRET measurement, which comprises the following steps:
| 09-03-2009 |
| 20090220989 | Particle-Based Analyte Characterization - Methods for assaying a sample for an analyte are provided. In various embodiments, the methods comprise contacting a sample suspected of containing the analyte with a non-uniform particle comprising a capture molecule, and further contacting the particle with a detection moiety comprising a label that permits detection of the analyte when associated with the particle. The methods may be performed to detect and/or quantitate analyte in the sample. In some embodiments, the methods may be performed in an automated manner, and may use an optical and/or cytometric apparatus for performing the method(s). The methods may further be performed with automated vessel-processing apparatus(es), such as plate loaders, plate washers, etc. Also provided are complexes containing the described materials formed by an assay of the invention, including excited state complexes. Kits useful for performing such methods are also provided. | 09-03-2009 |
| 20090233315 | MAGE-C2 antigenic peptides and uses therefor - This invention relates to isolated peptides derived from MAGE-C2, nucleic acid molecules that encode MAGE-C2 and the isolated peptides derived from MAGE-C2, expression vectors comprising the nucleic acid molecules, host cells transformed or transfected with the nucleic acid molecules or the expression vectors, and to tetramers comprising the peptides, HLA molecules, ∃ | 09-17-2009 |
| 20090239240 | Mutant Forms of Fas Ligand and Uses Thereof - The invention provides for DNA encoding Fas ligand muteins and chimeras and the proteins encoded thereby. The invention further includes the use of DNA and vectors to produce transformed cells expressing the mutant or chimeric Fas ligand. When the Fas ligand of the invention is a non cleavable form, the cells expressing the Fas ligand are useful in vitro for identifying Fas expressing cells or in vivo for reducing populations of Fas expressing cells. Thus, in other embodiments, the present invention is also directed to a method for treating a patient, for example a mammal, for autoimmune disease or transplant rejection by administering a Fas ligand therapeutic agent. The therapeutic agent is a polypeptide, a polynucleotide encoding the polypeptide or a small molecule. The polypeptides include full-length Fas ligand polypeptide, or a biologically active variant, derivative, portion, fusion or peptide thereof. | 09-24-2009 |
| 20090239241 | METHODS AND COMPOSITIONS FOR DIAGNOSIS, STRATIFICATION, AND MONITORING OF ALZHEIMER'S DISEASE AND OTHER NEUROLOGICAL DISORDERS IN BODY FLUIDS - The inventors have discovered a collection of proteinaceous biomarkers (“AD biomarkers) which can be measured in peripheral biological fluid samples to aid in the diagnosis of neurodegenerative disorders, particularly Alzheimer's disease and mild cognitive impairment (MCI). The invention further provides methods of identifying candidate agents for the treatment of Alzheimer's disease by testing prospective agents for activity in modulating AD biomarker levels. | 09-24-2009 |
| 20090246802 | ANNEXIN-BASED APOPTOSIS MARKERS - The present invention describes an annexin derivative and a method of using the annexin derivative as a biosensor for real-time visualization of phosphatidylserine exposure, apoptosis, live-cell imaging and monitoring of cell health. | 10-01-2009 |
| 20090253148 | FLUORESCENCE POLARIZATION hERG ASSAY - Disclosed are assays, methods, and kits for the screening of test compounds for their capability to induce cardiotoxicity in a subject. In particular, whether a test compound has the effect to prolong the Q-T interval as measured by an electrocardiogram in a human. The assays, methods, and kits disclosed herein make use of the binding interaction between novel fluorescent tracers and the hERG K | 10-08-2009 |
| 20090263828 | Novel assay for precursor T-cells having high proliferative capacity (PHPC-asay) - Diagnostic assay of antigen-specific T-cell precursors to determine the immunological status of patients suffering in chronic infectious diseases and to anticipate the disease progression and the response to therapy | 10-22-2009 |
| 20090269783 | B7-H2 molecules, novel members of the B7 family and uses thereof - Novel B7-like polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length B7-like proteins, the invention further provides isolated B7-like fusion proteins, antigenic peptides, and anti-B7-like antibodies. The invention also provides B7-like nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a B7-like gene has been introduced or disrupted. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided. | 10-29-2009 |
| 20090275049 | EphA4 POLYPEPTIDE HAVING A NOVEL ACTIVITY AND USE THEREOF - The present invention provides a polynucleotide which consists of a partial sequence of a polynucleotide encoding the amino acid sequence as shown in SEQ ID NO: 2, wherein the former polynucleotide encodes a polypeptide consisting of a partial amino acid sequence of SEQ ID NO: 2 whose N-terminus starts between positions 564 and 621 of SEQ ID NO: 2. | 11-05-2009 |
| 20090275050 | 14273 receptor, a novel G-protein coupled receptor - The present invention relates to a newly identified receptor belonging to the superfamily of G-protein-coupled receptors. The invention also relates to polynucleotides encoding the receptor. The invention further relates to methods using the receptor polypeptides and polynucleotides as a target for diagnosis and treatment in receptor-mediated disorders, specifically, cardiovascular diseases, including congestive heart failure. The invention further relates to drug-screening methods using the receptor polypeptides and polynucleotides to identify agonists and antagonists for diagnosis and treatment. The invention further encompasses agonists and antagonists based on the receptor polypeptides and polynucleotides. The invention further relates to procedures for producing the receptor polypeptides and polynucleotides. | 11-05-2009 |
| 20090275051 | LUMINOGENIC AND NONLUMINOGENIC MULTIPLEX ASSAY - A method to detect the presence or amount of at least one molecule for an enzyme-mediated reaction in a multiplex luminogenic/nonluminogenic assay is provided. | 11-05-2009 |
| 20090280506 | SF, A NOVEL FAMILY OF TASTE RECEPTORS - The invention provides isolated nucleic acid and amino acid sequences of taste cell specific G-protein coupled receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of taste cell specific G-protein coupled receptors. | 11-12-2009 |
| 20090286259 | SCREENING METHOD FOR GPCR LIGANDS - This invention relates to a method of identifying a compound capable of binding to a target domain of a G-protein coupled receptor comprising the steps of: (a) providing a receptor comprising said target domain and a first group linked to said target domain; (b) bringing into contact said receptor of (a) with a test molecule comprising a second group and said compound linked to each other, wherein said first group binds said second group; and (c) determining, subsequent to the binding of said first group to said second group, whether said compound binds to said target domain. | 11-19-2009 |
| 20090291457 | RE-TARGETED TOXIN CONJUGATES - The present invention provides a method for designing a re-targeted toxin conjugate for use in treating a medical condition or disease. Also provided, is the use of said conjugates in the manufacture of a medicament for treating medical conditions or diseases. The conjugates include a Targeting Moiety, which directs the conjugate to a desired target cell, and are characterised by a Targeting Moiety that increases exocytic fusion in the target cell. The present invention also provides methods for identifying agonists suitable for use as Targeting Moieties, and methods for preparing conjugates comprising said Targeting Moieties. | 11-26-2009 |
| 20090298091 | Modulation of T Cell Recruitment - The instant invention is based, at least in part, on the discovery that T-bet controls ThI cell recruitment to sites of inflammation. This invention pertains to, inter alia, methods of identifying agents that modulate the effects of T-bet on the recruitment of T cells to sites of inflammation by modulating P-selectin-mediated T cell rolling and/or stable adherence of a T cell to a vascular endothelial cell, as well as methods of use therefore. | 12-03-2009 |
| 20090298092 | ANALYTICAL SYSTEM, AND ANALYTICAL METHOD AND FLOW STRUCTURE THEREOF - An analytical system includes a working fluid, a uniform dividing unit and a separating unit. The working fluid includes a first component and a second component with different characteristics. The uniform dividing unit is utilized to uniformly divide the working fluid and relatively rotated with respect to a reference axis. Under a capillarity force as well as the result of Coriolis force and siphon force, the first component can be separated from the second component by the separating unit. | 12-03-2009 |
| 20090305303 | IMMUNOCHROMATOGRAPHY DEVICE FOR THE DIAGNOSIS OF DISEASES IN A SAMPLE - An immunochromatography device for determining the presence of a binding analyte in a biological sample of a subject, includes the steps of a sample application site for receiving the biological sample; at least one conjugate pad allowing the binding analyte present in the biological sample to bind to a labeled agent; and at least one test pad comprising assay areas, wherein the sample application site is adapted to direct at least part of said biological sample of a subject through the at least one conjugate pad into at least three assay areas of the at least one test pad and at least one of the at least one test pad comprises two assay areas. | 12-10-2009 |
| 20090305304 | High Resolution Label Free Analysis of Cellular Properties - The invention provides methods of detecting a change in cell growth patterns. | 12-10-2009 |
| 20090305305 | Method and probes for the detection of a tumor-specific fusion protein - The invention relates to the detection of fusion proteins. Described are a set of at least a first and a second molecular probe, each probe provided with a dye wherein the dyes together allow energy transfer, at least one probe provided with a reactive group allowing juxtaposing at least the first and second probes wherein the reactive group allows modulation of juxtaposing the probes such that there is an increased likelihood of energy transfer between the dyes. A method is provided which permits detecting the presence of a fusion protein in a cell at the single cell level. | 12-10-2009 |
| 20090311717 | MICROFLUIDIC CHIP DESIGN COMPRISING CAPILLARIES - The invention provides a microfluidic device wherein capillaries are connected to the microfluidic device by a deformable penetrable substance. | 12-17-2009 |
| 20090311718 | DEGRADED AGONIST ANTIBODY - The invention relates to a modified antibody which contains two or more H chain V regions and two or more L chain V regions of monoclonal antibody and can transduce a signal into cells by crosslinking a cell surface molecule(s) to thereby serve as an agonist. The modified antibody can be used as a signal transduction agonist and, therefore, useful as a preventive and/or remedy for various diseases such as cancer, inflammation, hormone disorders and blood diseases. | 12-17-2009 |
| 20090317827 | Receptor In Dendritic Cells - The present invention provides the use of GPR91 as a target in dendritic cells. | 12-24-2009 |
| 20090317828 | Diagnostic Kits to Detect SP22 and SP22 Antibodies - Oral, topical and injectable contraceptives, which are based on sperm protein 22 kDa (SP22) polypeptides and antibodies and infertility diagnostics and kits are provided. | 12-24-2009 |
| 20090317829 | High-throughput assay for sugar-mediated drug transport - The invention provides a rapid, quantitative assay to directly assess the impact of a diverse range of sugars upon the sugar-mediated uptake of corresponding sugar-conjugates into various cell types. | 12-24-2009 |
| 20090325192 | Rapid particle detection assay - The present invention proves instruments and methods for detecting and/or quantitating an analyte in a fluid sample. The fluid sample is placed in a sample chamber having a small, shallow detection region. The analyte is magnetically labeled using magnetic particles coated with a binding reagent, and is detectably labeled using a fluorescent dye or other detection reagent. The magnetically labeled analyte is concentrated into the detection region using a focusing magnet positioned underneath the sample chamber detection region. Concentrated analyte is measured using excitation optics positioned on top of the sample chamber detection region, adapted to illuminate only the detection region, and detection optics positioned on top of the detection region, adapted to detect only light emitted from the detection region. In a preferred embodiment, the invention provides a simple, rapid assay for measuring the concentration of CD4 | 12-31-2009 |
| 20100003699 | TISSUE CARBOHYDRATE COMPOSITIONS AND ANALYSIS THEREOF - The present invention reveals novel methods for producing novel carbohydrate compositions, glycomes, from animal tissues. The tissue substrate materials can be total tissue samples and fractionated tissue parts, or artificial models of tissues such as cultivated cell lines. The invention is further directed to the compositions and compositions produced by the methods according to the invention. The invention further represent methods for analysis of the glycomes, especially mass spectrometric methods. | 01-07-2010 |
| 20100003700 | Optoelectronic sensor - A device for detecting the presence of an antigen including (1) a cell having antibodies which are expressed on the surface of the cell and are specific for the antigen to be detected, where binding of the antigen to the antibodies results in an increase in calcium concentration in the cytosol of the cell, the cell further having a emitter molecule which, in response to the increased calcium concentration in the cytosol, emits a photon; (2) a liquid medium for receiving the antigen and in which the cell is immersed; and (3) an optical detector arranged for receiving the photon emitted from the cell. | 01-07-2010 |
| 20100009383 | Method for the simple and rapid detection of cells and biomolecules by means of paramagnetic particles - The invention relates to a simple and rapid method for the detection of cells and biomolecules by means of paramagnetic particles (beads) without separating the detected biomolecules or target cells from the beads before evaluating the specific bond. | 01-14-2010 |
| 20100009384 | ACTR-1, a novel human acyltransferase and uses thereof - The invention provides isolated nucleic acid molecules, designated ACTR-1 nucleic acid molecules, which encode novel acyltransferase family members. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing ACTR-1 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which an ACTR-1 gene has been introduced or disrupted. The invention still further provides isolated ACTR-1 proteins, fusion proteins, antigenic peptides and anti-ACTR-1 antibodies. Diagnostic methods utilizing compositions of the invention are also provided. | 01-14-2010 |
| 20100009385 | ASSAYS AND ENHANCERS OF THE HUMAN DELTA ENAC SODIUM CHANNEL - This invention relates to electrophysiological assays that measure sodium conductance activity of a delta human epithelial sodium channel (ENaC) in the presence and absence of delta hENaC enhancers. Also, the invention generally relates to assays for identifying compounds that enhance the activity of delta hENaC, especially in an oocyte expression system. These compounds have potential application in modulating (enhancing) salty taste perception. | 01-14-2010 |
| 20100009386 | METHODS OF IDENTIFICATION, ASSESSMENT, PREVENTION AND THERAPY OF LUNG DISEASES AND KITS THEREOF - The invention provides biomarkers and combinations of biomarkers that are useful in diagnosing lung diseases such as non-small cell lung cancer or reactive airway disease. The invention also provides methods of differentiating lung disease, methods of monitoring therapy, and methods of predicting a subject's response to therapeutic intervention based on the extent of expression of the biomarkers and combinations of biomarkers. Kits comprising agents for detecting the biomarkers and combination of biomarkers are also provided. | 01-14-2010 |
| 20100009387 | Detection of early stages and late stages HPV infection - Embodiments of the invention provide methods, monoclonal antibodies, polyclonal antibodies, assays, and kits for detecting HPV infection and HPV related cancer diagnosis, including infection by various HPV genotypes, early and/or late stage HPV-associated or HPV-specific cancers. Various specific or pan monoclonal antibodies recognizing specific epitope for specific HPV protein or HPV type, or common epitope for various HPV proteins or HPV types are obtained. The invention also provides one or more solid surface to coat the testing cell lysate. Also, the anti-HPV antibody can be coated on the solid surface of the invention to capture HPV proteins and detect HPV infection. | 01-14-2010 |
| 20100021937 | METHOD FOR DETECTING PATHOGENS USING MICROBEADS CONJUGATED TO BIORECOGNITION MOLECULES - A method and system are provided for the simultaneous detection and identification of multiple pathogens in a patient sample. The sample is combined with microbeads, which have been injected with quantum dots or fluorescent dye and conjugated to pathogen-specific biorecognition molecules, such as antibodies and oligonucleotides. Treatment options may be determined based on the identities of the pathogens detected in the sample. | 01-28-2010 |
| 20100021938 | Diagnosis and Prediction of Alzheimer's Disease - Alzheimer's disease (AD) is characterized by the accumulation of amyloid-β peptide (Aβ) in the brain. Aβ is derived from amyloid precursor protein (APP) by β- and γ-secretases, with the β form (sAPPβ) being associated with the disease state, and the α form (sAPPα) being associated with the non-disease state. The present inventor proposes that defined the ratio of sAPPα to sAPPβ or the ratio of CTFα to CTFβ provide and accurate diagnosis of the disease, as well as a predictor for asymptomatic patients at risk of developing AD. In addition, drug screening and monitoring of treatment effectiveness can exploit this same sAPPα/sAPPβ or CTFα/CTFβ ratio. | 01-28-2010 |
| 20100035277 | METHOD AND APPARATUS FOR DETERMINING THE CELL ACTIVATION OF A TARGET CELL BY AN ACTIVATOR - The invention relates to a method and a device for determining the cell activation of a target cell by an activator, said method having the following steps: provision of a probe measuring device with a probe sample arrangement having a measuring probe and a sample holder; loading of the probe sample arrangement with a target cell and with an activator assigned to the target cell, the measuring probe being loaded with the activator, and the sample holder being loaded with the target cell, or vice versa; relative mutual displacement of the measuring probe and the sample holder until contact is made between the target cell and the activator by means of a displacement apparatus of the probe measuring device; recording of measurement values, indicating binding between the target cell and the activator, for the measuring probe with the probe measuring device during the relative displacement of the measuring probe and the sample holder; and determination of a dimension for the cell activation of the target cell from the measurement values recorded. | 02-11-2010 |
| 20100035278 | BIOLOGICAL SUBSTANCE ANALYSIS CHIP, BIOLOGICAL SUBSTANCE ANALYSIS KIT AND METHOD OF BIOLOGICAL SUBSTANCE ANALYSIS USING THEM - The present invention provides a biological substance analysis chip capable of quantitative measurement in a minute region through immobilization of a biological substance trapping probe to a substrate surface in a more uniform density, a biological substance analysis kit that uses said chip, and a biological substance analysis method that uses the above. | 02-11-2010 |
| 20100041067 | Reaction Sensing in Living Cells - Chemical reactions occurring within a living cell are measured in a manner that does not affect the viability of the cell or the reaction under study. In one embodiment, one or more sensors are introduced into the cell and/or covalently associated with the exterior cell membrane. The sensor(s) emit an observable signal indicating a value of a parameter associated with the chemical reaction, e.g., the concentration of a reaction product. Because cell viability is not compromised, the cell may be stimulated (e.g., by subjection to an agonist or antagonist, a pathogen, a pharmaceutical compound, or a potential toxin) so as to affect the reaction under study. | 02-18-2010 |
| 20100047823 | Polypeptides - The use of polypeptides capable of binding to PtdIns(3,4)P | 02-25-2010 |
| 20100055714 | IKB kinase, subunits thereof, and methods of using same - The present invention provides an isolated nucleic acid molecules encoding IκB kinase (IKK) catalytic subunit polypeptides, which are associated with an IKK serine protein kinase that phosphorylates a protein (IκB) that inhibits the activity of the NF-κB transcription factor, vectors comprising such nucleic acid molecules and host cells containing such vectors. In addition, the invention provides nucleotide sequences that can bind to a nucleic acid molecule of the invention, such nucleotide sequences being useful as probes or as antisense molecules. The invention also provides isolated IKK catalytic subunits, which can phosphorylate an IκB protein, and peptide portions of such IKK subunit. In addition, the invention provides anti-IKK antibodies, which specifically bind to an IKK complex or an IKK catalytic subunit, and IKK-binding fragments of such antibodies. The invention further provides methods of substantially purifying an IKK complex, methods of identifying an agent that can alter the association of an IKK complex or an IKK catalytic subunit with a second protein, and methods of identifying proteins that can interact with an IKK complex or an IKK catalytic subunit. | 03-04-2010 |
| 20100062460 | Novel fluorochromes for organelle tracing and multi-color imaging - Provided are compounds, methods and kits for identifying in cells of interest organelles including nuclei and a wide variety of organelles other than nuclei (non-nuclear organelles), as well as cell regions or cell domains. These compounds and methods can be used with other conventional detection reagents for identifying the location or position or quantity of organelles and even for distinguishing between organelles in cells of interest. | 03-11-2010 |
| 20100062461 | MULTIPLEX DETECTION OF CELL SURFACE RECEPTORS OR IMMOBILIZED ANTIGENS - The present invention provides an antibody-based multiplex detection method for identifying one or more epitopes in one or more samples, the method comprising the steps of: (a) contacting each of the samples comprising the epitopes with one or more antibodies specific to the epitopes to be detected; (b) washing unbound antibodies away and eluting bound antibodies from the epitope carriers to provide separate antibody eluates; and (c) detecting and quantifying antibodies in the antibody eluates to generate separate profiles of antibodies for each of the samples. The epitopes to be detected can be located intracellularly, or present on the surface of cells or an artificial surface. In general, the epitopes are derived from protein, polypeptides with or without post-translational modification, carbohydrate, nucleic acid, or lipid. | 03-11-2010 |
| 20100075341 | STANDARDIZED EVALUATION OF THERAPEUTIC EFFICACY BASED ON CELLULAR BIOMARKERS - The present invention provides materials and methods for predicting the response of a disease state to a therapeutic agent. A targeting moiety specific for a biological marker is labeled with a reporter moiety and used to analyze cells characteristic of the disease state. The output of the reporter moiety, which may be fluorescence intensity, is compared to the output of reference standard analyzed under similar or identical conditions. The use of a reference standard allows biomarker reporting to be normalized. Biomarker values can then be correlated from sample to sample and from laboratory to laboratory based on quantitative calibration on a universal reference standard. | 03-25-2010 |
| 20100086943 | METHOD FOR THE DETECTION OF POST-TRANSLATIONAL MODIFICATIONS - Method for the detection in homogeneous medium of a post-translational modification of a protein substrate catalyzed by a cell enzyme, characterized in that the post-translational modification reaction takes place in intact living cells, in that these cells comprise a heterologous expression vector coding for a fusion protein comprising the protein substrate and a first coupling domain and in that it comprises the following stages: (v) Incubation of the cells in the presence or in the absence of a compound to be tested capable of modulating the activity of said enzyme, (vi) Addition to the reaction medium of a first fluorescent compound member of a first pair of FRET partners covalently bonded to a coupling agent capable of binding specifically to the first coupling domain present on the protein substrate, (vii) Addition to the reaction medium of a second fluorescent compound member of this first pair of FRET partners, and covalently bonded to a binding domain specific to the site of the protein substrate having undergone the post-translational modification and not binding to the non-modified protein substrate, (i) Measurement of the FRET signal emitted by the sample, this signal being representative of the quantity of protein substrate having undergone said post-translational modification; and cells for the implementation of said method. | 04-08-2010 |
| 20100093000 | Tyrosine phosphorylation sites - The invention discloses novel phosphorylation sites identified in carcinoma and/or leukemia, peptides (including AQUA peptides) comprising a phosphorylation site of the invention, antibodies specifically bind to a novel phosphorylation site of the invention, and diagnostic and therapeutic uses of the above. | 04-15-2010 |
| 20100105075 | SYSTEMS AND METHODS OF VOLTAGE-GATED ION CHANNEL ASSAYS - Systems and methods are provided for optically measuring ion concentrations in biological samples. The systems and methods employ polymer-based optical ion sensors that include ion-selective ionophores and a pH sensitive chromionophore. Electrodes are providing for electrically stimulating the biological samples. | 04-29-2010 |
| 20100120060 | METHOD FOR DETERMINING MOLECULES OR MOLECULE PARTS IN BIOLOGICAL SAMPLES - The invention relates to a method for determining molecules, molecule groups, and/or molecule parts in biological samples. The method includes spiking the sample at least once with at least one light emitting marker and measuring the light emission of the marker. Herein, it is intended that the light emission inherent to the sample is reduced or eliminated by means of bleaching prior to measuring the light emission of the marker. | 05-13-2010 |
| 20100120061 | RAPID IMMUNOCHROMATOGRAPHIC DETECTION BY AMPLIFICATION OF THE COLLOIDAL GOLD SIGNAL - The present invention relates to a rapid immunochromatographic test device suitable to detect an antibody and/or antigen in a sample, uses of said device for detecting diseases in a sample, a method for the production of said device as well as a kit comprising the device. | 05-13-2010 |
| 20100120062 | RHIZOME VIABILITY DETERMINATION METHOD - A method of determining the viability of a rhizome comprises the sequential steps of: (a) sampling material from the rhizome; (b) contacting material sampled in step (a) with an effective amount of a formulation comprising a first viability indicator; and (c) observing the action of the first viability indicator on the sampled rhizome material, to determine the viability of the rhizome. The method may further comprise one or more additional sequences involving the additional steps of: (d) contacting material sampled in step (a) with an effective amount of a formulation comprising a further viability indicator different to that used in step (b); and (e) observing the action of the further viability indicator on the sampled rhizome material, to determine the viability of the rhizome. In this way, an overall assessment of the viability of the rhizome can be made based on the action of a plurality of viability indicators, each relying on an interaction with different reagents in the plant cells. | 05-13-2010 |
| 20100120063 | GPCR Arrestin Assays - Sensitive assays for candidate compounds affecting GPCR activity are provided using a cell containing fusion proteins comprising a first fusion protein comprising (a) a target GPCR fused to a small fragment of β-galactosidase through a linker comprising a phosphorylation site or (b) a GPCR or a protein of interest, where the GPCR and protein of interest form a complex and one of them is fused to the small fragment of β-galactosidase; and a second fusion protein comprising arrestin fused to a large fragment of β-galactosidase. In (a), the affinity of the small and large fragments is optimized based on the background to signal ratio and the absolute signal observed. The assay is performed using a β-galactosidase substrate that provides a detectable optical signal. | 05-13-2010 |
| 20100143939 | Intracellular antibodies - The invention related to intracellular single domain immunoglobulins, and to a method for determining the ability of an immunoglobulin single domain to bind to a target in an intracellular environment, comprising the steps of: a) providing a first molecule and a second molecule, wherein stable interaction of the first and second molecules leads to the generation of a signal; b) providing a single intracellular immunoglobulin domain which is associated with the first molecule, said single immunoglobulin domain being free of complementary immunoglobulin domains; c) providing an intracellular target which is associated with the second molecule, such that association of the immunoglobulin domain and the target leads to stable interaction of the first and second molecules and generation of the signal; and d) assessing the intracellular interaction between the immunoglobulin domain and the target by monitoring the signal. | 06-10-2010 |
| 20100151491 | CHEMICAL SURFACE NANOPATTERNS TO INCREASE ACTIVITY OF SURFACE-IMMOBILIZED BIOMOLECULES - The present invention has been achieved in order to solve the problems which may occur in the nanopatterning of biomolecules with an aim to improve the activity and bio-recognition properties of the surface-immobilized biomolecules. A structure for bio-detection according to one aspect of the present invention comprises a large-scale chemical nanopattern of fouling and non-fouling areas fabricated on a homogeneous surface of the structure; and a biomolecule confined to the fouling area. | 06-17-2010 |
| 20100151492 | METHODS FOR THE TREATMENT OF INFECTIONS AND TUMORS - PD-1 antagonists are disclosed that can be used to reduce the expression or activity of PD-1 in a subject. An immune response specific to an infectious agent or to tumor cells can be enhanced using these PD-1 antagonists in conjunction with an antigen from the infectious agent or tumor. Thus, subjects with infections, such as persistent infections can be treated using PD-1 antagonists. In addition, subjects with tumors can be treated using the PD-1 antagonists. In several examples, subjects can be treated by transplanting a therapeutically effective amount of activated T cells that recognize an antigen of interest and by administering a therapeutically effective amount of a PD-1 antagonist. Methods are also disclosed for determining the efficacy of a PD-1 antagonist in a subject administered the PD-1 antagonist. In some embodiments, these methods include measuring proliferation of memory B cells in a sample from a subject administered the PD-1 antagonist. | 06-17-2010 |
| 20100151493 | MULTICOLOR FLOW CYTOMETRY COMPOSITIONS CONTAINING UNCONJUGATED PHYCOBILIPROTEINS - Novel compositions containing (i) ligand conjugated phycobiliprotein tandem dyes and (ii) unconjugated phycobiliproteins are provided wherein the phycobiliproteins are derived from the same bacterial or eukaryotic algae species. The phycobiliproteins must be the same or different, but must contain some non-crosslinked subunits which exchange. Also provided are methods for preparing these compositions, kits containing these compositions or components of the same, and methods of using these compositions for cellular and non-cellular analysis. | 06-17-2010 |
| 20100173328 | Apparatus for measuring a cell number and a quantity of a cellular protein expression and the method thereof - The apparatus and method thereof for harmlessly and continuously measuring and recording a target protein expression and a number of growing cells are provided. By causing an AC current to flow through an electrode where cells grows thereon, the target protein expression and the number of growing cells are obtained via converting the impedance values of the electrode. | 07-08-2010 |
| 20100173329 | Exposed proliferation-related peptides, ligands and methods employing the same - Novel thymidine kinase (TK1) derived peptide consisting of the amino acid sequence of SEQ ID NO:1 or SEQ ID NO:2 is employed to obtain ligands having specificity to the peptide. The ligand may be an antibody or fragment thereof and may be used in various methods and kits for health screening and the like. | 07-08-2010 |
| 20100184087 | Haptens, hapten conjugates, compositions thereof and method for their preparation and use - A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, a thiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels. | 07-22-2010 |
| 20100190186 | Method for Pathogen Capture from Mammalian Blood - Disclosed is a method for isolating, and thereby concentrating, microorganisms from mammalian blood. The method may be used in conjunction with PCR amplification of the DNA/RNA of the microorganism(s) to identify pathogenic microorganisms within the blood without requiring that those organisms be cultured as a first step in the identification process. | 07-29-2010 |
| 20100196925 | Methods for Screening Cells and Antibodies - The invention provides methods of detecting a change in cell growth patterns, methods of screening many different antibodies in one receptacle, and methods of detecting specific binding of an antibody to a protein or cell, wherein the antibody is in a mixture of many different antibodies. | 08-05-2010 |
| 20100203552 | INTERACTION OF BIM WITH TRIM2 - Methods, compositions, and cells for drug screening based on interaction between a Bim polypeptide and a TRIM2 polypeptide. Methods and compositions for treating cancer based on tested levels of Bim and TRIM2 proteins are also provided. | 08-12-2010 |
| 20100216159 | SWITCHABLE AFFINITY BINDERS - Methods and kits for binding and releasing biological targets, comprising, a binder comprising an environmentally reactive molecular switch that can switch between a high affinity state, to bind the target, to a low affinity state, to release the target. | 08-26-2010 |
| 20100227334 | Reaction sensing in living cells - Chemical reactions occurring within a living cell are measured in a manner that does not affect the viability of the cell or the reaction under study. In one embodiment, one or more sensors are introduced into the cell and/or covalently associated with the exterior cell membrane. The sensor(s) emit an observable signal indicating a value of a parameter associated with the chemical reaction, e.g., the concentration of a reaction product. Because cell viability is not compromised, the cell may be stimulated (e.g., by subjection to an agonist or antagonist, a pathogen, a pharmaceutical compound, or a potential toxin) so as to affect the reaction under study. | 09-09-2010 |
| 20100240066 | COMPOUNDS AND METHODS FOR MODULATING CADHERIN-MEDIATED PROCESSES - Peptides comprising a cadherin cell adhesion recognition (CAR) sequence, and compositions comprising such peptides, are provided. Methods of using such peptides for modulating cadherin-mediated processes in a variety of therapeutic contexts are also provided. Methods are also provided for identifying compounds that are capable of modulating cadherin-mediated processes. | 09-23-2010 |
| 20100255499 | COMPOSITIONS AND METHODS FOR TRANSPORT OF MOLECULES WITH ENHANCED RELEASE PROPERTIES ACROSS BIOLOGICAL BARRIERS - Conjugates of a cargo molecule with a transporter molecule are disclosed, where the cargo molecule and the transporter molecule are linked covalently by a releasable linker. The cargo of the conjugate can be a biologically active agent or a reporter molecule. The transporter modulates the transport of the cargo across a biological barrier (e.g., a cell membrane) compared to the transport of the unconjugated cargo. Releasable linkers suitable for rapid and facile conjugation to various types of cargo and transporters are also disclosed, along with methods for using the linkers in the synthesis of conjugates. | 10-07-2010 |
| 20100261197 | Method, System, and Compositions for Cell Counting and Analysis - The present invention provides a low cost imaged-based system for detecting, measuring and/or counting labeled features of biological samples, particularly blood specimens. In one aspect, the invention includes a system for imaging multiple features of a specimen that includes one or more light sources capable of successively generating illumination beams each having a distinct wavelength band and a plurality of differentially excitable labels capable of labeling a specimen comprising multiple features, such that each different feature is labeled with a different differentially excitable label. System of the invention may further include a controller operationally associated with the one or more light sources for successively directing illumination beams onto the specimen so that each of the different differentially excitable labels is successively caused to emit an optical signal within the same wavelength band, an optical system capable of collecting such emitted optical signals and forming successive images corresponding to the labeled features of the specimen on a light-responsive surface to form successive sets of image data thereof, and a disposable cuvette for collection and optical analysis of non-red blood cells. | 10-14-2010 |
| 20100273184 | DEVICE FOR MAGNETIC DETECTION OF INDIVIDUAL PARTICLES IN A MICROFLUID CHANNEL - A device dynamically detects particles of a fluid. The device can be miniaturized for detecting and selecting magnetized particles, particularly cells. | 10-28-2010 |
| 20100273185 | Detection of Biased Agonist Activation - The invention provides single assay methods to detect the activity of biased agonists or agonists that are less than full agonists on cells. | 10-28-2010 |
| 20100279312 | METHOD OF SCREENING APOPTOSIS ACCELERATING COMPOUND OR ANTI-APOPTOTIC COMPOUND AND METHOD OF DETERMINING MALIGNANCY OF NEURODEGENERATIVE DISEASE - Screening methods for determining pro-apoptotic compounds or anti-apoptotic compounds comprise measuring the interaction between p53 and NEDL1 in the presence and in the absence of a test compound, and comparing the strength of interaction between p53 and NEDL1 in the presence and in the absence of the test compound. | 11-04-2010 |
| 20100279313 | Novel secreted immunomodulatory proteins and uses thereof - The invention concerns cDNA molecules encoding TANGO 191 and TANGO 195, both of which are transmembrane proteins. | 11-04-2010 |
| 20100291588 | SYSTEMS AND METHODS INCLUDING SELF-CONTAINED CARTRIDGES WITH DETECTION SYSTEMS AND FLUID DELIVERY SYSTEMS - Methods, systems, and apparatus for detecting the presence of analytes are described. A fluid or gas sample may pass through a microsieve-based detection system and/or a particle-based detection system of a cartridge. Detection and analysis techniques may be applied to determine the identity and quantity of the captured analytes. | 11-18-2010 |
| 20100291589 | COMBINATION THERAPY FOR THE TREATMENT OF DIABETES AND CONDITIONS RELATED THERETO AND FOR THE TREATMENT OF CONDITIONS AMELIORATED BY INCREASING A BLOOD GLP-1 LEVEL - The present invention concerns combination of an amount of a GPR119 agonist with an amount of a dipeptidyl peptidase IV (DPP-IV) inhibitor such that the combination provides an effect in lowering a blood glucose level or in increasing a blood GLP-1 level in a subject over that provided by the amount of the GPR119 agonist or the amount of the DPP-IV inhibitor alone and the use of such a combination for treating or preventing diabetes and conditions related thereto or conditions ameliorated by increasing a blood GLP-1 level. The present invention also relates to the use of a G protein-coupled receptor to screen for GLP-1 secretagogues. | 11-18-2010 |
| 20100297664 | PARATOPE AND EPITOPE OF ANTI-MORTALIN ANTIBODY - The amino acid sequences of paratope regions involved in internalizing function of an anti-mortalin antibody into tumor cells were determined for the L-chain and H-chain variable regions of cellular internalizing anti-mortalin antibodies and non-internalizing anti-mortalin antibodies. Cancer-cell-specific drug delivery is provided by using the mortalin-binding activity of a single-chain antibody (scFv) wherein L-chain and H-chain variable regions both having the paratope region are linked together via a peptide linker. Also, the sequence of 6 amino acids of an epitope to be recognized by an anti-mortalin antibody having the internalizing function was determined. With the use of an expression vector comprising a nucleic acid that encodes the epitope, an agent for accelerating internalization of a mortalin antibody, a drug bound thereto, and the like into cancer cells is provided. | 11-25-2010 |
| 20100304403 | MULTIPLEX ASSAY METHODS AND COMPOSITIONS - Luminescence assays and compositions for assay of biomolecular interaction and activity and detection of modulators of biomolecular interaction and activity are provided. Technology described herein has utility in a variety of assay formats and types, for example, simultaneous monitoring multiple parameters which affect interaction and activity of biological molecules. Compositions and methods are provided herein which include a first solid-phase support associated with a first specific binding agent and a photosensitizer; a second solid-phase support associated with a second specific binding agent and a first emission system; and a third solid-phase support associated with a third specific binding agent and a second emission system. | 12-02-2010 |
| 20100317028 | BIOASSAY METHOD FOR YOKUKANSAN - The invention intends to find out an in-vitro bioassay system capable of ensuring qualities of yokukansan to a higher degree, and provides a bioassay method for yokukansan, comprising competitively reacting labeled ligand and a test sample containing yokukansan with cells or cell membranes expressing serotonin 1A receptors, and measuring binding activity of yokukansan from the amount of the labeled ligand bound, and a bioassay method for yokukansan, comprising reacting labeled GTP and a test sample containing yokukansan with cells or cell membranes expressing serotonin 1A receptors, and measuring receptor-agonist activity of yokukansan from the amount of the labeled GTP bound. | 12-16-2010 |
| 20110014629 | METHODS FOR IMPROVING THE RECOVERY OF TROPONIN I AND T IN MEMBRANES, FILTERS AND VESSELS - A method to facilitate recovery troponin I and/or troponin T from a sample comprising addition of troponin C to the sample or to a surface from which the troponin I and/or troponin T are recovered. | 01-20-2011 |
| 20110014630 | Fluorescent Carbon Nanoparticles - Disclosed are photoluminescent particles. The particles include a core nano-sized particle of carbon and a passivation agent bound to the surface of the nanoparticle. The passivation agent can be, for instance, a polymeric material. The passivation agent can also be derivatized for particular applications. For example, the photoluminescent carbon nanoparticles can be derivatized to recognize and bind to a target material, for instance a biologically active material, a pollutant, or a surface receptor on a tissue or cell surface, such as in a tagging or staining protocol. | 01-20-2011 |
| 20110027800 | Derivatives of 1,2-dihydro-7-hydroxyquinolines Containing Fused Rings - The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the dyes of the invention are useful fluorescent dyes. The invention includes chemically reactive dyes, dye-conjugates, and the use of such dyes in staining samples and detecting ligands or other analytes. | 02-03-2011 |
| 20110065124 | Novel genes encoding proteins having prognostic, diagnostic, preventive, therapeutic, and other uses - The invention provides isolated TANGO 239, TANGO 219, TANGO 232, TANGO 281, A236 (INTERCEPT 236), TANGO 300, TANGO 353, TANGO 393, TANGO 402, TANGO 351 and TANGO 509 nucleic acid molecules and polypeptide molecules. The invention also provides antisense nucleic acid molecules, expression vectors containing the nucleic acid molecules of the invention, host cells into which the expression vectors have been introduced, and non-human transgenic animals in which a nucleic acid molecule of the invention has been introduced or disrupted. The invention still further provides isolated polypeptides, fusion polypeptides, antigenic peptides and antibodies. Diagnostic, screening and therapeutic methods utilizing compositions of the invention are also provided. | 03-17-2011 |
| 20110065125 | CONTROLLED PLATELET ACTIVATION TO MONITOR THERAPY OF ADP ANTAGONISTS - A method is provided of determining whether an individual has reduced ability to form platelet thrombi due to inhibition of platelet activation initiation, signal transduction and/or GPIIb/IIIa blockade. A blood sample is obtained from the individual being assessed. The blood sample is mixed in combination with 1) an anticoagulant; 2) sufficient buffer to maintain the pH and salt concentration of the anticoagulated blood within a range suitable for platelet aggregation; 3) a platelet GPIIb/IIIa receptor ligand immobilized on a solid surface; 4) one or more agents to enhance a signal transduction pathway and 5) a receptor activator. The combination is incubated under conditions for agglutinating particles. Platelet-mediated agglutination is assessed in the agitated mixture. The absence of agglutination indicates that the individual has a reduced ability to form platelet thrombi. | 03-17-2011 |
| 20110065126 | SUBSTANCE-IMMOBILIZING SUBSTRATE, SUBSTANCE-IMMOBILIZED SUBTRATE, AND ANALYSIS METHOD - The present invention relates to a substance-immobilizing substrate for immobilizing a substance to be detected by chemiluminescence. The substance-immobilizing substrate of the present invention comprises a metal portion composed of at least one metal selected from the group consisting of chromium and molybdenum, or an alloy of the metal on at least a portion of a surface of the substrate. The present invention further relates to a substance-immobilized substrate wherein a substance is immobilized on the substance-immobilizing substrate. The substance is immobilized on the metal portion and the substrate is used for detecting the immobilized substance by chemiluminescence. The present invention further relates to an analysis method comprising directly or indirectly detecting by chemiluminescence a substance that is immobilized on a substrate. The substrate comprises a metal portion composed of at least one metal selected from the group consisting of chromium and molybdenum, or an alloy of the metal on at least a portion of a surface of the substrate, and the substance is immobilized on the metal portion. | 03-17-2011 |
| 20110081658 | T1R3 BINDING ASSAYS - Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in taste signaling, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for representing taste perception of a particular tastant in a mammal are also described, as are methods for generating novel molecules or combinations of molecules that elicit a predetermined taste perception in a mammal, and methods for simulating one or more tastes. Further, methods for stimulating or blocking taste perception in a mammal are also disclosed. | 04-07-2011 |
| 20110091903 | METHOD OF ANALYZING A SAMPLE FOR A BACTERIUM USING DIACETYLENE-CONTAINING POLYMER SENSOR - The invention relates to methods of analyzing a sample for a bacterium of interest. In particular, the methods involve an initial capture process that includes the use of one or more antibodies having antigenic specificities for one or more distinct analytes characteristic of the specific bacterium. After initial capture of a specific bacterium, techniques of analyzing involve colorimetric techniques, particularly using colorimetric sensors that include polydiacetylene (PDA) materials. | 04-21-2011 |
| 20110091904 | METHOD FOR DETERMINATION OF AFFINITY AND KINETIC CONSTANTS - The invention is related to a method for quantification of a first dissociation equilibrium constant K | 04-21-2011 |
| 20110097735 | FLUORESCENT PYRENE COMPOUNDS - The present invention relates to fluorescent pyrene dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions. | 04-28-2011 |
| 20110104711 | GPR81-Ligand complexes and their preparation and use - Complexes of GPR81 receptor components and ligand components, such as L-lactate or GHB, may be used as an assay reagent for screening for modulators of GPR81 receptor activity. | 05-05-2011 |
| 20110111426 | METHOD OF BIOASSAYING YOKUKANSAN - The invention intends to find out a bioassay system with an in-vitro test capable of ensuring the higher quality of yokukansan, and provides a bioassay method for yokukansan, comprising competitively reacting a labeled ligand and yokukansan with cells or cell membranes expressing glutamate receptors, measuring the binding activity of yokukansan, and evaluating the pharmacological activity value of yokukansan from the measurement value. | 05-12-2011 |
| 20110129850 | MICROFLUIDIC PLATFORM FOR CELL CULTURE AND ASSAY - A microfluidic chip for at least one of cell culturing and cell assay has a cell culture chamber defined by the microfluidic chip, a first microchannel defined by the microfluidic chip and constructed to provide a fluid path to said cell culture chamber, the microchannel having a pneumatic valve formed therein to permit selective opening and closing of a fluid path to said cell culture chamber, and a second microchannel defined by the microfluidic chip and constructed to provide a fluid path from the cell culture chamber. | 06-02-2011 |
| 20110129851 | Methods for Obtaining Bioactive Compounds from Phytoplankton - Phytoplankton represent a potential source of bioactive compounds. The present disclosure provides, inter alia, methods for identifying glycerolipids and apoptosis-inducing sphingosine-like lipids from virally-infected phytoplankton. | 06-02-2011 |
| 20110143367 | ANTIBODIES AGAINST APRIL AS BIOMARKERS FOR EARLY PROGNOSIS OF LYMPHOMA PATIENTS - The invention relates to antibodies directed against APRIL (A Proliferation Inducing TNF Ligand, also known as TALL-2), in particular the monoclonal antibody Aprily-2, hybridoma cells producing monoclonal antibody Aprily-2, and the use of a combination of an antibody against membrane-anchored APRIL and Aprily-2 in the diagnosis of B cell lymphoma resistance to treatment and the prognosis of clinical development of Diffuse Large B-Cell (DLBCL) lymphoma from high risk patients (>60 years and International Prognostic Index >2). An amino acid sequence GTGGPSQNGEGYP called Stalk, useful in the preparation of antibodies, is described. | 06-16-2011 |
| 20110151480 | METHODS OF PURIFYING ZSIG33 - The present invention relates to a method of forming a peptide-receptor complex with zsig33 polypeptides and their receptors as well as antibodies. Methods of modulating gastric contractility, nutrient uptake, growth hormones, the secretion of digestive enzymes and hormones, and/or secretion of enzymes and/or hormones in the pancreas are also included. | 06-23-2011 |
| 20110159519 | OPTICAL BIOSENSORS - Provided are biosensors, compositions comprising biosensors, and methods of using biosensors in living cells and organisms. The biosensors are able to be selectively targeted to certain regions or structures within a cell. The biosensors may provide a signal when the biosensor is targeted and/or in response to a property of the cell or organism such as membrane potential, ion concentration or enzyme activity. | 06-30-2011 |
| 20110159520 | HYBRID PROTEINS COMPRISING MEMBRANE RECEPTOR AND ION CHANNEL, AND THEIR USE AS BIOSENSORS - The present invention relates to the use of a hybrid protein including the sequence of a first membrane receptor fused at its C-terminus to the N-terminus of an ion channel, and possibly containing a linker between the C-terminus of the first membrane receptor and the N-terminus part of the ion channel, the linker being absent in the natural configuration of the first membrane receptor and the ion channel, as a biosensor for: the screening of drugs modulating the activity of the first membrane receptor in its natural configuration, and/or for the in vitro diagnosis of pathologies associated with the presence or the variation of amount of a molecule modifying the activity of the first membrane receptor in its natural configuration. | 06-30-2011 |
| 20110177525 | ANTIBODIES AND METHODS OF DIAGNOSING DISEASES - The present invention generally relates to antibodies and use of these antibodies in diagnostic assays for various disease states, including cancer. In certain embodiments, the invention provides an isolated human or humanized antibody or functional fragment thereof including an antigen-binding region that is specific for an epitope on a protein, in which the epitope is specific to a tissue or body fluid and the epitope is indicative of a disease. | 07-21-2011 |
| 20110189695 | METHOD AND DEVICE FOR PRODUCING METAL-CONTAINING ORGANIC COMPOUNDS - The invention relates to a method and to a device for producing conjugates comprising or consisting of a metallic nanoparticulate component and an organic component. Said method enables activated or reactive nanoparticles containing metal to be produced by irradiating a metal body with a laser beam and prevents the modification and damage of organic components of said conjugates by laser irradiation. Said nanoparticulate metallic component comprises plasmon resonant metal. According to the invention, the claimed method of production enables particles having a metal centre and a metal-oxide covering to be produced, in particular when using a carrier fluid containing oxygen, e.g. alcohol or water. | 08-04-2011 |
| 20110189696 | MONOCLONAL ANTIBODY STRO-4 - The present invention relates to a monoclonal antibody designated STRO-4 which specifically binds human and ovine HSP-90beta and its use for enriching multipotential cells such a mesenchymal precursor cells (MPCs). | 08-04-2011 |
| 20110195431 | HUMAN ORPHAN G PROTEIN-COUPLED RECEPTORS - The invention disclosed in this patent document relates to transmembrane receptors, more particularly to endogenous, human orphan G protein-coupled receptors. | 08-11-2011 |
| 20110201020 | T1R HETERO-OLIGOMERIC TASTE RECEPTORS AND CELL LINES THAT EXPRESS SAID RECEPTORS AND USE THEREOF FOR IDENTIFICATION OF TASTE COMPOUNDS - The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners. | 08-18-2011 |
| 20110207146 | METHODS AND COMPOSITIONS FOR DETECTING RECEPTOR-LIGAND INTERACTIONS IN SINGLE CELLS - The invention provides methods and compositions for simultaneously detecting the activation state of a plurality of proteins in single cells using flow cytometry. The invention further provides methods and compositions of screening for bioactive agents capable of coordinately modulating the activity of a plurality of proteins in single cells. The methods and compositions can be used to determine the protein activation profile of a cell for predicting or diagnosing a disease state, and for monitoring treatment of a disease state. | 08-25-2011 |
| 20110229910 | Fluorescent Substrates for Neurotransmitter Transporters - The invention is based on the finding that IDT307 and analogs thereof are fluorescent substrates transported by several neurotransmitter transporters. Provided are methods for the analysis of neurotransmitter transport and binding using IDT307 and its analogs. The invention also provides rapid methods for screening for modulators of neurotransmitter transport. | 09-22-2011 |
| 20110236907 | RADIOLABLED CYCLOPAMINE ASSAY FOR THE SMOOTHENED RECEPTOR - The present invention discloses novel methods for screening compositions for both agonist and antagonist activity on the smoothened receptor. The assay tests ligands on the activity of the receptor using a cyclopamine binding and ultra high receptor expression. | 09-29-2011 |
| 20110250616 | ELECTROACTIVE SURFACE-CONFINABLE MOLECULES - The invention provides compositions, kits, methods, and species that include electroactive entities which can serve as signaling entities in chemical and/or biochemical assays. The electroactive species can be metallocenes, such as ferrocenes, including substituents that affect the oxidation/reduction potential (redox potential) of the species. By controlling the redox potential of the species, multiple species can be used in a single assay, each species having a different redox potential, for simultaneous signaling of different binding events. Additionally, species having redox potentials lower than 490 mV can be provided, allowing signaling within a potential range easily detectable in the presence of biological fluids. | 10-13-2011 |
| 20110250617 | DEVICE AND ANALYZING SYSTEM FOR CONDUCTING AGGLUTINATION ASSAYS - A device for conducting an agglutination assay comprising several reaction vessels, each reaction vessel comprising an upper chamber having an opening for accepting reactants and/or a sample; and a lower chamber comprising an end in communication with the upper chamber for receiving fluids from the upper chamber, a closed end opposite to the end, and a matrix for separating agglutinates from non-agglutinates; wherein the device further comprises a rotating support able to rotate around an axis and holding pivotally the reaction vessels in a way to allow the reaction vessels to pivot about an axis essentially perpendicular to the rotation axis of the support when the latter is rotated, such that the fluids remain in the upper chamber when the support is not rotated, and can flow from the upper chamber to the lower chamber and into the matrix when the support is rotated. | 10-13-2011 |
| 20110256552 | METHOD FOR PREPARING A SUBSTRATE FOR IMMOBILIZING A CELL, SAID SUBSTRATE AND USES THEREOF - The present invention relates to a method of preparation of a solid substrate capable of immobilizing at least one cell and/or at least one part of a cell, said method comprising a step consisting of fixing, to said solid substrate, a fusogenic compound capable of being inserted in cell membranes. The present invention also relates to a method for immobilizing at least one cell and/or at least one part of a cell using the solid substrate thus prepared, said solid substrate and its uses in the area of biomedical diagnostics or health monitoring of biological fluids or intended for human or animal use. | 10-20-2011 |
| 20120015376 | Free Solution Measurement Of Molecular Interactions By Backscattering Interferometry - Disclosed are methods, systems, and apparatuses for the free solution measurement of molecular interactions by backscattering interferometry (BSI). Molecular interaction can be detected between analytes in free-solution wherein at least one of the analytes is label-free and detection is performed by back-scattering interferometry. Further, molecular interaction can be detected between analytes in free-solution, wherein at least one of the analytes is label-free, wherein one of the analytes is present in a concentration of less than about 5.0×10 | 01-19-2012 |
| 20120034622 | ACTIVATION AND MONITORING OF CELLULAR TRANSMEMBRANE POTENTIALS - The use of nanostructures to monitor or modulate changes in cellular membrane potentials is disclosed. | 02-09-2012 |
| 20120034623 | UP-CONCENTRATION OF ORGANIZ MICROOBJECTS FOR MICROSCOPIC IMAGING - A method of analyzing a sample fluid containing organic microobjects is proposed. The method comprises the steps of: up-concentrating (S | 02-09-2012 |
| 20120040370 | SYSTEMS AND METHODS FOR RAPIDLY CHANGING THE SOLUTION ENVIRONMENT AROUND SENSORS - The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention also provides a system and methods for modulating, controlling, preparing, and studying receptors. | 02-16-2012 |
| 20120077207 | CD86 and CD80 receptor competition assays - The present invention discloses a method for assaying the binding of L104EA29YIg to a receptor. The receptor is preferably CD86 or CD80. The present invention also discloses antibodies to be used in the assay, as well as hybridomas expressing the antibodies. | 03-29-2012 |
| 20120122115 | Bacterial quorum sensing biosensor - The present invention generally relates to fluorescent resonance energy transfer protein compounds and methods for using such compounds as biosensors. The present invention also relates to one or more nucleic acids for encoding the protein compounds, vectors containing the nucleic acids, cells transformed by the vectors, and methods for making and using the foregoing compositions. | 05-17-2012 |
| 20120156689 | METHOD AND APPARATUS FOR DETERMINING AN ANALYTE PARAMETER - A method of determining an analyte parameter is described for an analyte in a detection region having at least one analyte binding zone. The method includes detecting presence of an analyte element bound at the binding zone, incrementing or decrementing a count and preventing recount of the element to obtain absolute quantification. | 06-21-2012 |
| 20120231474 | CONSTRUCTS THAT ALLOW FOR DETECTION AND QUANTITATION OF MEMBRANE PROTEINS - A construct is described that allows for detection and quantitation of membrane-bound polypeptides. | 09-13-2012 |
| 20120270230 | MUTANT G-PROTEIN COUPLED RECEPTORS AND METHODS FOR SELECTING THEM - The invention relates to mutant G-protein coupled receptors with increased conformational stability, and methods of use thereof. In some aspects, polynucleotides encoding the mutant G-protein coupled receptors are provided. In some aspects, host cells comprising the polynucleotides are provided. In some aspects, the invention relates to crystallized forms of the mutant G-protein coupled receptors, and methods of preparing the same. | 10-25-2012 |
| 20120270231 | LATTICE-MISMATCHED CORE-SHELL QUANTUM DOTS - The disclosure relates to lattice-mismatched core-shell quantum dots (QDs). In certain embodiments, the lattice-mismatched core-shell QDs are used in methods for photovoltaic or photoconduction applications. They are also useful for multicolor molecular, cellular, and in vivo imaging. | 10-25-2012 |
| 20120270232 | CHARACTERIZATION OF GRANULOCYTIC EHRLICHIA AND METHODS OF USE - The present invention relates, in general, to methods of screening a sample obtained from a subject for antibodies relating to granulocytic ehrlichia (GE) infection. | 10-25-2012 |
| 20120329069 | METHODS FOR ANTIBODY ENGINEERING - The invention provides a method for identifying positions of an antibody that can be modified without significantly reducing the binding activity of the antibody. In many embodiments, the method involves identifying a substitutable position in a parent antibody by comparing its amino acid sequence to the amino acid sequences of a number of related antibodies that each bind to the same antigen as the parent antibody. The amino acid at the substitutable position may be substituted for a different amino acid without significantly affecting the activity of the antibody. The subject methods may be employed to change the amino acid sequence of a CDR without significantly reducing the affinity of the antibody of the antibody, in humanization methods, or in other antibody engineering methods. The invention finds use in a variety of therapeutic, diagnostic and research applications. | 12-27-2012 |
| 20130078644 | Post-Translational Modifications Identified by Elemental Analysis - Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enyzmes | 03-28-2013 |
| 20130084581 | Method of Identifying Transmembrane Protein-Interacting Compounds - A method for screening compounds for their ability to interact with transmembrane proteins is provided. Also provided is a method for determining whether proteins such as transmembrane proteins are able to oligomerise. | 04-04-2013 |
| 20130210028 | Biological Assays Using Microparticles - Encoded microparticles described are useful in the study of many different biological agents in multiplex assays. For instance, the encoded microparticles may be employed in various co-precipitation assays to purify and/or isolate various analytes of interest. Encoded microparticles may also be used as real-time detectors in many different situations whereby binding of a secreted analyte or contaminating analyte may be detected using various labeling techniques. Further, encoded microparticles may be attached in a specific manner to particular cell types, for instance in a heterogeneous mixture of cells, either fixed in tissue or circulating, to allow identification, localization and/or sorting of the cells in the context of various biological events under various environments or conditions. | 08-15-2013 |
| 20130230864 | METHOD FOR AND USE OF DIGITAL HOLOGRAPHIC MICROSCOPY AND IMAGING ON LABELLED CELL SAMPLES - The present invention relates to use of a digital holographic microscopy and imaging setup and a method of digital holographic microscopy and imaging for detecting molecules or structures stained or labelled to at least one cell or conjugated to antibodies which are bound either directly to said at least one cell or indirectly via another or several antibodies in a chain bound to said at least one cell. | 09-05-2013 |
| 20130273561 | LIPID ENCAPSULATION OF SURFACE ENHANCED RAMAN SCATTERING (SERS) NANOPARTICLES - Phospholipid-microvesicle-encapsulated surface enhanced Raman scattering (SERS) nanoparticles and methods for making the encapsulated particles are described. The encapsulated particles can be used in nanomedicine. Four Raman-active species were used. A bilayer was observed by TEM, and the SERS spectrum of each dye species (SERS reporter) was confirmed. | 10-17-2013 |
| 20130288268 | SULFENIC ACID-REACTIVE COMPOUNDS AND THEIR METHODS OF SYNTHESIS AND USE IN DETECTION OR ISOLATION OF SULFENIC ACID-CONTAINING COMPOUNDS - The present invention provides compounds of Formula I: | 10-31-2013 |
| 20130309689 | METHODS AND REAGENTS FOR BIOMOLECULE LABELING, ENRICHMENT AND GENTLE ELUTION - Methods and sets of non-biological reagents (elution reagents, tag isomers, tag reactive groups, crosslinkers) for single or multiplexed capture and gentle elution of biomolecules. Examples are provided using amine- and cysteine-reactive reagents for enrichment of proteins, peptides, and rare peptide modifications. | 11-21-2013 |
| 20130309690 | TETRAMERIC ALPHA-SYNUCLEIN AS BIOMARKERS - The present invention provides the surprising finding that alpha-synuclein exists in vivo as a folded tetramer. Provided are various methods and technologies that arise from this finding, including methods and kits for identifying individuals susceptible to or suffering from certain diseases, disorders or conditions associated with stability of alpha-synuclein tetramers, and/or individuals likely (or not) to respond to therapy with agents that alter level and/or stability of alpha-synuclein tetramers. | 11-21-2013 |
| 20130316366 | EXPRESSION OF SECRETED AND CELL-SURFACE POLYPEPTIDES - Some embodiments herein provide compositions and methods for expressing secreted and cell-surface-bound polypeptides in a single cell. In some embodiments, secreted and cell-surface polypeptide are produced from a single polynucleotide. The polynucleotide can comprise a sequence (or sequence encoding a polypeptide) that mediates separation of a membrane anchor from the polypeptide. In some embodiments, a desired ratio of secreted to surface-bound polypeptide is obtained by selecting a sequence that mediates a desired level of separation of the membrane anchor from the polypeptide. | 11-28-2013 |
| 20130316367 | Discrete Contact MR Bio-Sensor with Magnetic Label Field Alignment - The invention describes a family of sensors for assaying macro-molecules and/or biological cells in solution. The invention also describes methods of making and using the sensors. Each sensor has the form of a well (a hollow cylinder having a floor but no lid) or a trench whose walls comprise a plurality of GMR or TMR devices. Suitably shaped magnets located below each well's floor pull labeled particles into the well/trench and up against the inner wall where a field gradient orients them for optimum detection. Any unattached labels that happen to also be in the well/trench are removed through suitably sized holes in the floor. | 11-28-2013 |
| 20130316368 | MULTI-TARGET PHOTONIC BIOSENSOR, AND METHOD FOR MANUFACTURING AND PREPARING SAME - A component or device is provided for the detection or the measurement in parallel of one or more specific types of biological or chemical target products. This component includes a group of nanotubes selected and/or functionalized to interact with the target product, around an optical waveguide. Thus, an optical coupling is produced between the optical waveguide and one or more optical characteristics of these nanotubes, the modifications of which are evaluated in the presence of the target product. In addition, a method is provided for manufacturing and preparing such a component or device, and a detection method using them, as well as a post-manufacture preparation method comprising a specific functionalization for different target products starting from the same type of pluripotent generic component. Also provided is a family of PFO-based functionalization polymers. | 11-28-2013 |
| 20130344502 | VIBRATING MICROPLATE BIOSENSING FOR CHARACTERISING PROPERTIES OF BEHAVIOUR BIOLOGICAL CELLS - There is described a system for testing a sample. The system comprises a microplate, at least one actuator, a plurality of mutually spaced sensors, and a processor. The microplate has a test portion including an interactive substance. The interactive substance is inherently interactive with a specified test substance. The microplate is arranged such that at least the test portion of the microplate may be brought into contact with the sample. The at least one actuator is operable to vibrate the microplate. The plurality of mutually spaced sensors are coupled to the microplate. Each sensor is operable to provide a respective sensory data time series during vibration of the microplate. The microplate and the sensors are arranged such that the provided sensory data time series are not independent from one another. The processor is operable to receive the sensory data time series from the sensors and to process the received sensory data time series so as to provide information about the test substance in the sample based on the sensed interaction between the test substance and the interactive substance on the test portion of the microplate. A corresponding method of testing a sample is also described. | 12-26-2013 |
| 20140057283 | IMPEDANCE BASED DEVICES AND METHODS FOR USE IN ASSAYS - A method for assaying target molecules in a sample liquid, the method comprising: providing an impedance monitoring device operably connected to an impedance analyzer; adding a sample liquid suspected of having target molecules to the well thereby permitting binding of target molecules to the capture molecules; monitoring impedance of the well; and determining the presence, amount or concentration of target molecules in the liquid sample from the monitored impedance. The device includes a nonconducting substrate having a well, at least two electrodes fabricated on a bottom of the well and on a same plane, wherein the surfaces of the at least two electrodes are modified with capture molecules configured to bind target molecules in a liquid sample, and at least two connection pads electrically connected to the at least two electrodes. | 02-27-2014 |
| 20140057284 | PHOTOLUMINESCENT MOLECULAR COMPLEX AND METHOD FOR DETERMINING OF THE CONCENTRATION OF SAID MOLECULAR COMPLEX - The invention concerns novel molecular complexes with photoluminescent probes whose specific association with purine-binding proteins leads to increased emission of long lifetime luminescence, and the application of the probes for monitoring activity of protein kinases (PKs) and other purine-binding proteins, screening of compounds as inhibitors of PKs and characterization of inhibitors targeted to the kinase, and methods of manufacturing of such probes. The invention concerns also the use of the improved method for monitoring activity of protein kinases in living cells, characterization of inhibitors of protein kinases, analysis of protein kinase-based disease biomarkers and other tasks of biological and medical importance. | 02-27-2014 |
| 20140065640 | Interferometric Detection Using Nanoparticles - This invention provides methods and systems for detecting interaction between members of a binding pair. The method involves associating one member of the binding pair with a nanoparticle and detecting the interaction between the two molecules by back-scattering interferometry. | 03-06-2014 |
| 20140113309 | METHOD AND APPARATUS FOR ANALYZING PROTEIN-PROTEIN INTERACTION ON SINGLE-MOLECULE LEVEL WITHIN THE CELLULAR ENVIRONMENT - A method of analyzing protein-protein interactions includes binding the first proteins to the substrate where the first proteins are tagged with the first markers which bind specifically to the biomolecules immobilized on the substrate or the first proteins bind specifically to the biomolecules immobilized on the substrate; incubating the substrate bound first proteins with cell lysate containing the second proteins which are tagged with second markers; analyzing the interactions between the first proteins and the second proteins in the cell lysate, and obtaining the first analytic value representing the kinetic picture of the interactions; incubating the substrate bound first proteins with cell lysate mixture of a cell lysate consisting of the second markers-tagged second proteins and another cell lysate comprising other proteins including unlabelled second proteins and obtaining the second analytic value; comparing and analyzing the first and the second analytic values. | 04-24-2014 |
| 20140127710 | BACKGROUND-FREE MAGNETIC FLOW CYTOMETRY - The invention relates to an apparatus and a method for magnetic flow cytometry, wherein magnetic units ( | 05-08-2014 |
| 20140170673 | UNITARY CARTRIDGE FOR PARTICLE PROCESSING - A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles. | 06-19-2014 |
| 20140220592 | METHOD OF CALIBRATION - The following processes are performed to improve the accuracy of the process of estimating the volume of a cell clump from an image including the cell clump. First, the image including the cell clump is acquired, and the optical density of the cell clump in the image is measured. Cross-section information about the cell clump is acquired by observation using a confocal microscope or by physical cutting. Based on the cross-section information, the vertical height of the cell clump is determined. Thereafter, data representing a relationship between the aforementioned optical density and the height is acquired. This improves the accuracy of the process of converting the optical density into the height to thereby achieve the accurate estimation of the volume of the cell clump. | 08-07-2014 |
| 20140234859 | CONTROLLED PLATELET ACTIVATION TO MONITOR THERAPY OF ADP ANTAGONISTS - A method is provided of determining whether an individual has reduced ability to form platelet thrombi due to inhibition of platelet activation initiation, signal transduction and/or GPIIb/IIIa blockade. A blood sample is obtained from the individual being assessed. The blood sample is mixed in combination with 1) an anticoagulant; 2) sufficient buffer to maintain the pH and salt concentration of the anticoagulated blood within a range suitable for platelet aggregation; 3) a platelet GPIIb/IIIa receptor ligand immobilized on a solid surface; 4) one or more agents to enhance a signal transduction pathway and 5) a receptor activator. The combination is incubated under conditions for agglutinating particles. Platelet-mediated agglutination is assessed in the agitated mixture. The absence of agglutination indicates that the individual has a reduced ability to form platelet thrombi. | 08-21-2014 |
| 20140302523 | Small-Molecule Hydrophobic Tagging of Fusion Proteins and Induced Degradation of Same - The present invention includes compounds that are useful in perturbing or disrupting the function of a transmembrane or intracellular protein, whereby binding of a compound to the transmembrane or intracellular protein induces proteasomal degradation of the transmembrane or intracellular protein. The present invention further includes a method of inducing proteasomal degradation of a transmembrane or intracellular protein. The present invention further includes a method of identifying or validating a protein of interest as a therapeutic target for treatment of a disease state or condition. | 10-09-2014 |
| 20150037813 | ANTIBODY AGAINST AFFINITY COMPLEX - The present invention provides a means and a method for specifically measuring a substance such as a small substance with high sensitivity by a sandwich method. Specifically, the present invention provides an antibody capable of specifically binding to an affinity complex and a method of measuring of the affinity complex comprising measuring the affinity complex using the antibody capable of specifically binding to the affinity complex. The antibody of the present invention may be a full-length antibody. The antibody of the present invention may also have a region derived from an immunoglobulin from an animal having an ability of gene conversion (e.g., a complementarity-determining region, a framework region, or a variable region). Examples of at least one factor that constitutes the affinity complex include a small substance or a protein (e.g., antibody). | 02-05-2015 |
| 20150056632 | FLUORESCENT DYES, FLUORESCENT DYE KITS, AND METHODS OF PREPARING LABELED MOLECULES - The present invention provides methods, compositions, and kits useful in preparing labeled molecules, which are useful in the detection of binding partners. | 02-26-2015 |
| 20150064713 | METHODS, KITS AND MEANS FOR DETERMINING INTRACELLULAR INTERACTIONS - Methods, kits and systems for determining whether a reaction occurs between a chimeric transmembrane receptor and an intracellular interaction partner thereof within a cell. | 03-05-2015 |
| 20150111222 | GENETICALLY ENCODED BIOSENSORS - The present disclosure provides, inter alia, genetically encoded recombinant peptide biosensors comprising analyte-binding framework portions and signaling portions, wherein the signaling portions are present within the framework portions at sites or amino acid positions that undergo a conformational change upon interaction of the framework portion with an analyte. | 04-23-2015 |
| 20150301048 | METHODS FOR DETECTING ALLOSTERIC MODULATORS OF PROTEIN - The present invention discloses, inter alia, methods for labeling a target protein with an SHG-active probe for detection by second harmonic or sum-frequency generation in order to identify agents which bind to an allosteric site on the target protein thereby altering its structural conformation | 10-22-2015 |
| 20150316481 | ENCAPSULATED DYE COATED NOBLE METAL NANOPARTICLES WITH INCREASED SURFACE ENHANCED RAMAN SCATTERING PROPERTIES AS CONTRAST AGENTS - The present disclosure provides semiconductor-metal composite nanoparticles with optical properties that are superior to those of pure materials for use as contrast agents. The composites include noble metal nanoparticles having a layer of linker molecules being bound to the surface of the noble metal nanoparticle and a layer of dye molecules bound to the layer of linker molecules. The dye molecules are selected such that they form an ordered structure that exhibits a collective absorption band shift, compared to the individual dye molecule, when bound to the noble metal nanoparticle. This structure is encapsulated in a stabilizing coating layer forming a multi-shell structure with properties suitable for biosensing and other detection applications which exhibit enhanced Raman scattering compared to nanoparticles having dye molecules bound thereto not in the ordered structure. | 11-05-2015 |
| 20150316557 | COUMARIN-BASED FLUOROGENIC AGENTS AND USES THEREOF FOR SPECIFIC PROTEIN LABELLING - There are provided fluorescent labelling agents comprising a dimaleimide core connected to a fluorophore, processes for preparation thereof, and uses thereof for labelling and/or detection of specific protein targets. Fluorescent labelling agents comprising a compound having the structure of Formula I, and salts thereof, are described. | 11-05-2015 |
| 20150355072 | Method for Enriching and Isolating Cells Having Concentrations Over Several Logarithmic Steps - For flow cytometric measurement, a channel includes a magnetic sensor and is disposed downstream of a chamber. The chamber and the channel form a closed system, wherein an axis of the channel extends along a flow direction of the channel. A closed system is present if the chamber merges directly into the channel. A magnet, more particularly a permanent magnet, and a deflection device, are both arranged at a predefined side of the channel. Here, the deflection device has at least one segment. Each segment is arranged in a concentration region of the channel. Each segment has a guide for guiding cells toward the axis. The deflection device thus enables an enrichment of magnetically marked cells, which are pulled to the channel side by the magnet in the respective concentration region, on the axis and guidance of these cells along the axis to the magnetic sensor. | 12-10-2015 |
| 20150377896 | Method of Identifying Transmembrane Protein-interacting Compounds - A method for screening compounds for their ability to interact with transmembrane proteins is provided. Also provided is a method for determining whether proteins such as transmembrane proteins are able to oligomerise. | 12-31-2015 |
| 20160033519 | BROMODOMAIN BINDING REAGENTS AND USES THEREOF - The present invention provides compounds of the Formula (I) and (II), and salts thereof, wherein L | 02-04-2016 |
| 20160041188 | PEPTIDE WITH GOLD BINDING AND EGFR RECEPTOR AFFINITY AND SAME ATTACHED TO GOLD NANOSTRUCTURE - An embodiment of the invention is a peptide comprising four domains, wherein domain I consists of thioctyl or monocytl, domain II consists of 2 to 3 positively charged amino acids selected from the group consisting of lysine and arginine, domain III consists of a dimeric ethylene unit; and domain IV comprises the peptide with SEQ ID No. 1 or a sequence having at least 90% identity to SEQ ID No. I. The peptide is preferably attached to a gold nanostructure, preferably a gold nanorod to provide an EFGR kit. An EFGR detection kit of the invention employs a gold nanostructure attached to a peptide sequence, the peptide sequence includes a binding sequence with an affinity toward EGFR, a ligand bound to gold atoms of the nanorod, a positively charged amino acid that maintains activity of the binding sequence, and a unit that increases hydrophilicity of the peptide sequence. | 02-11-2016 |
| 20160061847 | T2R Taste Receptors and Genes Encoding Same - Newly identified mammalian taste-cell-specific G Protein-Coupled Receptors and the genes encoding said receptors are described. Specifically, T2R taste G Protein-Coupled Receptors that are believed to be involved in bitter taste sensation, and the genes encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for representing taste perception of a particular tastant in a mammal are also described, as are methods for generating a novel molecules or combinations of molecules that elicit a predetermined taste perception in a mammal, and methods for simulating one or more tastes. | 03-03-2016 |
| 20160108015 | Carbofluorescein Lactone Ion Indicators and Their Applications - Fluorescent dyes useful for preparing fluorescent metal ion indicators, the fluorescent indicators themselves, and the use of the fluorescent indicators for the detection, discrimination and quantification of metal cations are provided. | 04-21-2016 |
| 20160123970 | MOLECULAR CYTOMETRY - The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions. | 05-05-2016 |
| 20160146782 | Diagnostic Method and System - The present invention relates to a method of diagnosis which identifies one or more individual cells and comprises determining at least one of a cell dimension and a cell area, and determining at least one of dark/light cell contrast characteristics, cell area characteristics, cell colour characteristics, cell roughness characteristics, distances between cell nuclei and cell convexity. A computer readable medium, a computer apparatus and a diagnostic system is also provided. | 05-26-2016 |
| 20160153952 | DETECTION AND RECOVERY OF CHEMICAL ELEMENTS FROM FLUIDS WITH TECTRABRACHION | 06-02-2016 |
| 20160161415 | METAL-ANTIBODY TAGGING AND PLASMA-BASED DETECTION - A target within a sample can be characterized using an energy source configured to transform a metal in the sample into a plasma and an optical spectroscopic detector configured to detect electromagnetic radiation emitted by the plasma to provide an optical-spectrum signal. A processor can determine presence of the metal in the sample using the optical-spectrum signal. The target can include a microbe or biological toxin. A recognition construct comprising a metal and a scaffold can be applied to the sample. The scaffold can bind to the target. Energy can be applied to transform at least some of the sample into a plasma. Electromagnetic radiation emitted by the plasma can be detected to provide an optical-spectrum signal of the sample. A preparation subsystem can add the recognition construct to the sample and a washing subsystem can wash unbound recognition construct out of the sample. | 06-09-2016 |
| 20160169876 | EXOSOME ANALYSIS METHOD, EXOSOME ANALYSIS CHIP, AND EXOSOME ANALYSIS DEVICE | 06-16-2016 |
| 20160169921 | MODULATING BACTERIAL MAM POLYPEPTIDES IN PATHOGENIC DISEASE | 06-16-2016 |
| 20160200785 | ENGINEERED OPSONIN FOR PATHOGEN DETECTION AND TREATMENT | 07-14-2016 |
| 20160376338 | HEAT-SENSING PROTEIN SWITCHES AND USES THEREOF - A region of the TRPV1 protein that functions as a temperature switch (FIG. | 12-29-2016 |
