Patent application number | Description | Published |
20100062495 | Homologous recombination-based DNA cloning methods and compositions - Methods and compositions for cloning a donor DNA molecule into an acceptor vector at a predetermined location are described. The methods are based on homologous recombination mediated by in vitro treatment of the donor DNA and the acceptor vector with an enzyme cocktail containing an exonuclease and a single-stranded DNA binding protein. | 03-11-2010 |
20100167356 | Homologous recombination-based DNA cloning methods and compositions - Methods and compositions for cloning a donor DNA molecule into an acceptor vector at a predetermined location are described. The methods are based on homologous recombination mediated by in vitro treatment of the donor DNA and the acceptor vector with an enzyme cocktail containing an exonuclease and a single-stranded DNA binding protein. | 07-01-2010 |
20100326828 | RAPID ELECTROPHORESIS BINDING METHOD AND RELATED KITS AND COMPOSITIONS - An improved staining method is described for staining a biopolymer such as a peptide, a protein, an RNA, a DNA, an oligosaccharide or a complex containing a peptide, a protein, an RNA, a DNA, or an oligosaccharide in a matrix. The method includes the step of moving a staining reagent into the matrix using an electric force. The staining time can be dramatically reduced relative to conventional technologies. The improved staining method can particularly be used, for example, to stain proteins after gel separation. Other related methods and related kits are also described. | 12-30-2010 |
20110081708 | Method of Sequence Optimization for Improved Recombinant Protein Expression using a Particle Swarm Optimization Algorithm - An improved gene sequence optimization method, the systematic optimization method, is described for boosting the recombinant expression of genes in bacteria, yeast, insect and mammalian cells. This general method takes into account of multiple, preferably most or all, of the parameters and factors affecting protein expression including codon usage, tRNA usage, GC-content, ribosome binding sequences, promoter, 5′-UTR, ORF and 3′-UTR sequences of the genes to improve and optimize the gene sequences to boost the protein expression of the genes in bacteria, yeast, insect and mammalian cells. In particular, the invention relates to a system and a method for sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm. The improved systematic optimization method can be incorporated into a software for more efficient optimization. | 04-07-2011 |
20120129239 | HOMOLOGOUS RECOMBINATION-BASED DNA CLONING COMPOSITIONS - Methods and compositions for cloning a donor DNA molecule into an acceptor vector at a predetermined location are described. The methods are based on homologous recombination mediated by in vitro treatment of the donor DNA and the acceptor vector with an enzyme cocktail containing an exonuclease and a single-stranded DNA binding protein. | 05-24-2012 |
20120289433 | SOLID SURFACE RESERVOIRS - An apparatus for forming a surface reservoir to hold a sample for a desirable period of time is described. The apparatus contains a platform, a solid surface disposed onto the platform, and an assembly of a bottomless vessel mounted on the solid surface. Also described is an apparatus that forms an array of surface reservoirs on a solid surface when multiple bottomless vessels are used, which can be used for high throughput applications. The apparatus can be used in applications on a solid surface, such as immunohistochemistry (IHC), oligo synthesis, peptide synthesis, ELISA, DNA array, peptide array, protein array, antibody array, tissue array, cell culturing, etc. | 11-15-2012 |
20120295294 | ENZYMATIC METHOD FOR PREPARING ASPARTAM - An improved method is described for the synthesis of aspartame using a condensation reaction between the alpha-carboxyl group of the L-aspartic acid and the amino group of the methyl L-phenylalaninate catalyzed by an enzyme. The method allowed efficient and cost effective production of aspartame. A method of identifying an enzyme useful for preparing aspartame is also described. | 11-22-2012 |
20130017574 | METHODS AND COMPOSITIONS FOR ENHANCED EXPRESSION AND SECRETION OF PROTEINS - Optimized signal peptide coding sequences for enhanced expression and secretion of protein from a cell and related compositions and methods are described. The optimized signal peptide coding sequence encodes an mRNA that contains at least one hairpin structure immediately downstream of the initiation codon. Methods for obtaining the optimized signal peptide coding sequences and methods for enhanced expression and secretion of proteins using the optimized signal peptide coding sequences are also described. | 01-17-2013 |
20130123118 | Metal Nanoparticle Structures For Enhancing Fluorescence-Based Arrays - Provided, among other things, is a multiplex assay comprising: conducting a fluorescence-developing assay on microtabs having at least one surface that shows plasmonic enhancement, wherein a plurality of the microtabs have unique probes affixed to their plasmonically enhanced surfaces; and measuring the fluorescence associated with the substrates and identifying the correlated probe by for the microtab. The microtabs can be, for example, MTPs that send a unique identifier, and the correlated probe can be identified by querying the MTPs for their identifier. | 05-16-2013 |
20130184436 | APPARATUS AND METHOD FOR SEMI-AUTOMATED PARALLEL SYNTHESIS OF PEPTIDES - An apparatus is provided for the semi-automated parallel synthesis of multiple peptides. The apparatus includes an array of nozzles, each positioned above or adjacent a separate reaction container, two or more liquid reservoirs, each reservoir coupled to the liquid dispenser(s), and base chamber(s) connected to the reaction containers for removing liquid there from. The addition and removal of liquids may be controlled by programmable electromagnetic valves. The apparatus may also be used for other parallel solid phase reactions. A method is also provided for synthesizing multiple polypeptides or other macromolecules, for example by using the above apparatus, wherein multiple common steps are performed automatically while reactants are added manually. | 07-18-2013 |
20140138249 | GEL ELECTROPHORESIS DEVICE FOR LOADING LARGE SAMPLE VOLUMES - A device for gel electrophoresis is provided having larger wells for loading an increased sample volume with improved gel resolution. The device includes a gel cassette having a front plate and a back plate, wherein at least one plate has a stepped inner surface to create a wider opening at the top of the gel cassette, a gel matrix, and a comb with teeth having a thickness substantially equal to the spacing at the top opening of the gel cassette. Using this device, the sample volume in each well can be increased and the sample height in each well can be significantly reduced, as compared to loading the same volume in the wells of a standard gel cassette. | 05-22-2014 |
Patent application number | Description | Published |
20080311602 | RAPID ELISA PROCESSES AND RELATED COMPOSITIONS - The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens. | 12-18-2008 |
20100075349 | RAPID ELISA PROCESSES AND RELATED COMPOSITIONS - The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens. | 03-25-2010 |
20110008807 | Composition Related to Rapid ELISA Process - The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens. | 01-13-2011 |