Patent application number | Description | Published |
20090055939 | Probe for detection and quantification of nitric oxide, and method for detecting and quantifying nitric oxide using the same | 02-26-2009 |
20090113563 | Probe for detecting nuclear receptor agonist or antagonist and method for screening agonist or antagonist to nuclear receptor with the use of the same - A probe for detecting an agonist or an antagonist to a nuclear receptor, in which, at least, a ligand-recognition site containing a ligand-binding domain of the nuclear receptor is connected with a binding-responsive site containing a peptide chain that specifically binds to a coactivator-binding site in the ligand-binding domain by a flexible linker to construct a fusion structure [ligand-recognition site/linker/binding-responsive site], and two reporters are connected with the respective ends of the fusion structure. | 04-30-2009 |
20090208970 | Probe for detection and quantification of inositol-1,4,5-trisphosphate and a method for detecting and quantifying inositol-1,4,5-trisphosphate using the same - A probe for detection and quantification with high accuracy in a noninvasive manner as to where and when inositol-1,4,5-trisphosphate is generated in living cells, and a method for detecting and quantifying inositol-1,4,5-trisphosphate using the probe. | 08-20-2009 |
20100184619 | METHOD FOR ANALYZING ORGANELLE-LOCALIZED PROTEIN AND MATERIAL FOR ANALYSIS - A method for analyzing an organelle-localized protein, which enables one to determine whether or not a test protein localizes to an organelle, comprising the steps of: (a) a step of introducing a fusion peptide (a), which comprises one half-peptide of an intein, one half-peptide of a fluorescent protein and an organelle-targeting signal peptide, into a eukaryotic cell; (b) a step of introducing a test protein bound to a fusion peptide (b), which comprises the other half-peptide of the fluorescent protein and the other half-peptide of the intein, into the eukaryotic cell; and (c) a step of detecting fluorescence signal emitted by the fluorescent protein, and a material for analysis to be used in such method are provided. | 07-22-2010 |
20100221719 | Probes for detecting protein nuclear transport and method for detecting and quantifying protein nuclear transport using the same - A convenient and highly accurate method for detecting protein nuclear transport induced by an endogenous or exogenous substance in local areas of living cells or animals is provided. The method uses a pair of probes for detecting protein nuclear transport, comprising Probe I and Probe II. In Probe I, a protein whose nuclear transport is to be detected or quantified is connected to an N-terminal end or a C-terminal end of a fusion protein [intein-C/reporter protein-C] wherein at least a C-terminal side polypeptide of an intein and a C-terminal side polypeptide of a reporter protein are connected in this order, and in Probe II, a nuclear localization signal is connected to an N-terminal end or a C-terminal end of a fusion protein [reporter protein-N/intein-N] wherein at least the remaining N-terminal side polypeptide of the reporter protein and the remaining N-terminal side polypeptide of the intein are connected in this order. | 09-02-2010 |
20100273150 | SINGLE MOLECULE-FORMAT BIOLUMINESCENT PROBE - This application aims to provide a single molecule-format probe for detecting a target-specific ligand easily and accurately as an index of the presence or absence of a signal. | 10-28-2010 |
20100297620 | ACTIVATED PROTEASE INDICATOR - It is an object of the invention to provide novel approaches capable of detecting activated protease and also detecting protease activation on real time at a high sensitivity in a noninvasive manner. By the method for detecting activated protease of the invention, an indicator in a circular form comprising the C-half fragment of luciferase (Luc-C) and the N-half fragment of luciferase (Luc-N) linked together through a substrate peptide for a protease is introduced in an in vitro assay system or in cells. Upon digestion of the substrate peptide by the protease, Luc-N and Luc-C together reconstructs active luciferase, so that the activated protease can be detected by assaying the luminescence signal from the luciferase. | 11-25-2010 |
20110312014 | PROBE FOR DETECTION AND QUANTIFICATION OF NITRIC OXIDE, AND METHOD FOR DETECTING AND QUANTIFYING NITRIC OXIDE USING THE SAME | 12-22-2011 |