Patent application number | Description | Published |
20090061416 | Surfaces and methods for biosensor cellular assays - Disclosed is an apparatus for measuring ligand-induced cell activity as defined herein, the apparatus including: an optical biosensor having a contact surface including a compatibilizer zone, an optional surface modifier zone, and a live cell zone. The disclosure also provides a method of making the apparatus and methods for measuring ligand-induced live cell activity with the apparatus. | 03-05-2009 |
20090093011 | Biosensors for ligand-directed functional selectivity - A system and method for determining ligand-directed functional selectivity of a receptor in a live-cell with a biosensor are disclosed. Also disclosed is a system and method for fragment-based screening with a cell-based, label-free biosensor functional assay. | 04-09-2009 |
20090093013 | Single-Cell Label-Free Assay - The disclosure provides a system and method for characterizing a single live-cell response to a stimulus with a biosensor imaging system having cells immobilized on the biosensor at a resolution level of a single cell, as defined herein. | 04-09-2009 |
20090093371 | MEMBRANE ARRAYS AND METHODS OF MANUFACTURE - The invention relates to G protein-coupled receptor (GPCR) microarrays on porous substrates for structural or functional analyses of GPCRs, and methods of preparing porous substrate surfaces for receiving membranes that comprise GPCRs. In one embodiment, a GPCR microarray of the invention comprises a membrane adhered to an upper surface of a porous substrate, the membrane spanning across a plurality of pores on the porous substrate to form a plurality of cavities having sufficient geometry to permit entry of assay reagents into each cavity, thereby allowing access of assay reagents to both sides of GPCR in the membrane. | 04-09-2009 |
20090098645 | Cell culture article and methods thereof - Disclosed is a cell culture article including: a substrate; a tie-layer attached to at least the substrate; and a bio-compatible layer attached to at least the tie layer, the bio-compatible layer having been obtained from surface oxidation of a polymer layer. Also disclosed are methods for making the cell culture article and methods for performing an assay of a ligand with the article. | 04-16-2009 |
20090142790 | Label Free Biosensors and Cells - Disclosed are compositions and methods for using label free optical biosensors for performing cell assays. In certain embodiments the assays can be performed in high throughput methods and can be multiplexed. | 06-04-2009 |
20090215650 | SUBSTRATES WITH STABLE SURFACE CHEMISTRY FOR BIOLOGICAL MEMBRANE ARRAYS AND METHOD FOR FABRICATING THEREOF - The present invention provides a method for preparing a physically stable array of biological membranes, including membrane proteins, on a surface, and the resultant article of manufacture. The method comprises providing a substrate; creating either a polar surface or reactive surface by coating the substrate with a material that either: (1) enhances the stability of lipid spots during withdrawing through a water/air interface and washing and drying protocols; or (2) gives rise to minimal non-specific binding of a labeled target to a background surface, and high specific binding to a probe receptor in said membrane array, or (3) both; and depositing an array of biological-membrane microspots on the substrate. The method may further comprise applying a reagent that includes a soluable protein to stabilize the biological membranes on the surface. Also provided is an article having biological-membrane microspots that are associated in a stable fashion with a substrate surface embodying these properties. | 08-27-2009 |
20090275074 | System and Method for Performing G Protein Coupled Receptor (GPCR) Cell Assays Using Waveguide-Grating Sensors - The present invention includes a system and method that uses optical LID biosensors to monitor in real time agonist-induced GPCR signaling events within living cells. Particularly, the present invention includes a system and method for using an optical LID biosensor to screen compounds against a target GPCR within living cells based on the mass redistribution due to agonist-induced GPCR activation. In an extended embodiment, the present invention discloses different ways for self-referencing the optical LID biosensor to eliminate unwanted sensitivity to ambient temperature, pressure fluctuations, and other environmental changes. In yet another extended embodiment, the present invention discloses different ways for screening multiple GPCRs in a single type of cell or multiple GPCRs in multiple types of cells within a single medium solution. In still yet another extended embodiment, the present invention discloses different ways to confirm the physiological or pharmacological effect of a compound against a specific GPCR within living cells. | 11-05-2009 |
20090275480 | UNIVERSAL READOUT FOR TARGET IDENTIFICATION USING BIOLOGICAL MICROARRAYS - A method and apparatus for implementing the method is provided. The method involves performing an indirect competitive binding assay on a microarray to identify biological or chemical targets and screen for compounds of interest. The microarray comprises a common ligand located among membrane-, lipid- or protein-associated active binding sites. The method takes advantage of known or well-characterized binding kinetics, and steric interference between biological or chemicals targets of interest and universal readout units for different binding sites within the limited confines of a microspot. The biological targets, chemicals or organisms can specifically bind to target-binding sites, while the universal readout unit binds to the ligands in the microspot. | 11-05-2009 |
20090298116 | CELL CULTURE APPARATUS HAVING DIFFERENT MICRO-WELL TOPOGRAPHY - A cell culture apparatus includes a substrate having formed therein a micro-well array, the micro-well array comprising a plurality of micro-wells. Each micro-well is defined by a curved surface which is concave. | 12-03-2009 |
20090298166 | CELL CULTURE APPARATUS HAVING VARIABLE TOPOGRAPHY - A cell culture apparatus includes a substrate having formed therein a micro-pillared well array. The micro-pillared well array includes a plurality of micro-pillared wells. Each micro-pillared structure includes a plurality of spaced-apart micro-pillars having distal ends shaped to form a well. The well is suitable for cell culture. | 12-03-2009 |
20090309617 | Biosensor antibody functional mapping - Disclosed is a system and method for measuring aspects of antibody function in live-cell systems as defined herein. The system and method also provide a method to measure prophylaxis or remedial aspects of antibody therapeutic candidates in a live-cell or a live-cell model. | 12-17-2009 |
20090325211 | System and method for dual-detection of a cellular response - A system and method as defined herein for dual-detection of evanescent-wave label-free light and evanescent-wave excited-fluorescent label-emitted light in an optical biosensor. | 12-31-2009 |
20100087332 | High-Throughput High-Information Content Label-Free Cell Biology Screening Methods - A method for hit compound identification in a high throughput, label-free biosensor cellular assay, one method including, for example:
| 04-08-2010 |
20100129854 | LIVER CELL TOXICITY ASSAY - The disclosure provides methods for characterizing the toxicity of a candidate molecule to liver cells as defined herein; methods of culturing metabolically active liver cells on a biosensor as defined herein; and biosensor liver culture systems as defined herein. | 05-27-2010 |
20100129856 | Methods and Devices for Cell Signaling Under Pulse Stimulation - The disclosure provides a device for measuring cellular responses under controlled environments. The disclosure also provides an array of devices for measuring cellular responses under controlled environments. The disclosure also provides methods to study cell signaling using the devices. By using microfluidics, the disclosure enables study of cell signaling under well-defined environment conditions. Such capability can be used for differentiating long-acting ligands from short-acting ligands, for determining the kinetics of receptor resensitization and functional recovery of receptor signaling, and for differentiating the sensitivity of a cell type to laminar flow-induced stress force. Through combination with conventional static label-free cell assays, the full spectrum of a drug compound can be studied in details, thus creating a complete representation of its pharmacology acting on living cells. | 05-27-2010 |
20100129908 | SPACED PROJECTION SUBSTRATES AND DEVICES FOR CELL CULTURE - An article for culturing cells includes a substrate on which cells can be cultured. The substrate has a base surface. An array of projections extends from the base surface. The projections have a height of about 1 micrometer to about 100 micrometers, and have a gap distance along the major surface from center to center between neighboring projections of about 10 micrometers to 80 micrometers. A plurality of arrays of projections may extend from the surface with gaps in the base surface between the arrays. Hepatocytes cultures on such microprojection array substrates maintained in vivo like morphology and membrane polarity. Hepatocytes co-cultured with helper cells on such substrates tended to grow in the area of the arrays, while the helper cells tended to grow in the areas between the arrays. | 05-27-2010 |
20100130725 | METHODS FOR CHARACTERIZING MOLECULES - Drug discovery is a complex undertaking facing many challenges, not the least of which is a high attrition rate as many promising candidates prove ineffective or toxic in the clinic owing to a poor understanding of the diseases, and thus the biological systems, they target. Therefore, it is broadly agreed that to increase the productivity of drug discovery one needs a far deeper understanding of the molecular mechanisms of diseases, taking into account the full biological context of the drug target and moving beyond individual genes and proteins. The present methods rely on the use of label-free cellular assays, particularly the DMR index, to systematically display the mode of actions, the toxicity, and the target(s) and pathway(s) of any molecules. | 05-27-2010 |
20100130736 | METHODS OF CREATING AN INDEX - Drug discovery is a complex undertaking facing many challenges, not the least of which is a high attrition rate as many promising candidates prove ineffective or toxic in the clinic owing to a poor understanding of the diseases, and thus the biological systems, they target. Therefore, it is broadly agreed that to increase the productivity of drug discovery one needs a far deeper understanding of the molecular mechanisms of diseases, taking into account the full biological context of the drug target and moving beyond individual genes and proteins. The present methods rely on the use of label-free cellular assays, particularly the DMR index, to systematically display the mode of actions, the toxicity, and the target(s) and pathway(s) of any molecules. | 05-27-2010 |
20100130829 | MATERIALS AND METHODS FOR DETECTING EARLY CELL MIGRATION EVENTS - Aspects relate to the field of label-free biosensors, including optical biosensors and electric biosensors, for studying cellular behaviour particularly early events in cell migration, under a concentration gradient of stimulus. Various embodiments include devices and methods that enable the generation of a concentration gradient of stimulus for cells contacting with the surface of a biosensor including a label free biosensor. Some aspects also disclose methods to detect cellular responses upon such concentration gradient of a stimulus, as well as methods to detect the potency and efficacy of a stimulus using a single biosensor. | 05-27-2010 |
20100152060 | ASSAY SOLUTION COMPOSITIONS AND METHODS FOR GPCR ARRAYS - Buffered assay solutions for performing 1) binding or 2) functional assays on GPCR arrays, along with methods for their use are described. The buffered assay solution has an underlying composition having: a buffer reagent with a pH in the range of about 6.5 to about 7.9; an inorganic salt of either a monovalent or divalent species, at a concentration from about 1 mM to about 500 mM; and optionally a combination of: c) a blocker reagent at a concentration of about 0.01 wt. % to about 2 wt. % of the composition, or d) protease-inhibitor at a concentration of about 0.001 mM to about 100 mM. In an embodiment for functional assay uses, the composition is modified to also include a GTP-analogue, a guanosine 5′-diphosphate (GDP) salt, and/or an anti-oxidant reagent. | 06-17-2010 |
20100184626 | ARRAYS OF BIOLOGICAL MEMBRANES AND METHODS AND USE THEREOF - The present invention overcomes the problems and disadvantages associated with prior art arrays by providing an array comprising a plurality of biological membrane microspots associated with a surface of a substrate that can be produced, used and stored, not in an aqueous environment, but in an environment exposed to air under ambient or controlled humidities. Preferably, the biological membrane microspots comprise a membrane bound protein. Most preferably, the membrane bound protein is a G-protein coupled receptor, an ion channel, a receptor serine/threonine kinase or a receptor tyrosine kinase. | 07-22-2010 |
20100323902 | Live-cell signals of pathogen intrusion and methods thereof - Disclosed is a system and method for measuring aspects of pathogen intrusion on a live-cell as defined herein. The system and method also provide a method to measure prophylaxis or remedial aspects of a therapeutic candidates in a live-cell or a live-cell model from pathogen intrusion. | 12-23-2010 |
20110020843 | LABEL-FREE METHODS RELATED TO PHOSPHODIESTERASES - 196. Disclosed are methods of incubating cells on biosensors, and methods using the disclosed incubation techniques to identify PDE4 modulators. | 01-27-2011 |
20110028345 | METHODS TO CHARACTERIZE CELL REPROGRAMMING AND USES THEREOF - Disclosed are label free biosensors and methods using these to observe stem cells and for the analysis of stem and related cells. | 02-03-2011 |
20110207789 | METHODS RELATED TO CASEIN KINASE II (CK2) INHIBITORS AND THE USE OF PURINOSOME-DISRUPTING CK2 INHIBITORS FOR ANTI-CANCER THERAPY AGENTS - Disclosed are methods related to label-free biosensor cellular assays to classify multienzyme complex modulators in live cells. Disclosed are also methods related to the identification of purinosome disrupting Casein Kinase II (CK2) inhibitors, and methods related to the use of purinosome disrupting CK2 inhibitors as therapeutic agents for modulating CK2 activity and purine synthesis pathway, and for improving prevention and treatment of CK2 associated cancers, viral infection and inflammation conditions. | 08-25-2011 |
20110230359 | LABEL-FREE ON-TARGET PHARMACOLOGY METHODS - Disclosed are methods and machines to determine on-target pharmacology of molecules using label-free biosensor cellular assays and label-free biosensor integrative pharmacology. | 09-22-2011 |
20110231103 | METHODS FOR DETERMINING MOLECULAR PHARMACOLOGY USING LABEL-FREE INTEGRATIVE PHARMACOLOGY - Disclosed are methods and machines to perform cluster analysis on label free biosensor data. | 09-22-2011 |
20110246078 | MITOCHONDRIA KATP ION CHANNEL AS A DRUG TARGET FOR PREVENTING LIVER DISEASES AND METHODS TO SCREEN MITOCHONDRIA KATP MODULATORS - Disclosed are compositions and methods related to modulation of K | 10-06-2011 |
20120015846 | Label-free Cellular Pharmacology For Drug Antitarget Assessment - Described are methods relating to assessing antitargets of molecules. Also described are methods of screening molecules. In some aspects of the methods, the molecules are analyzed using a label free biosensor. | 01-19-2012 |
20120022116 | COMPOSITIONS AND METHODS FOR THE TREATMENT OF PATHOLOGICAL CONDITION(S) RELATED TO GPR35 AND/OR GPR35-HERG COMPLEX - Disclosed are compositions and methods for the prevention and/or treatment of diseases which are pathophysiologically related to GPR35, and/or GPR35-hERG signaling complex. For example, disclosed are compounds for preventing and/or treating diseases which are pathophysiologically related to GPR35 in a subject. The compounds having a formula (I), (II) or (III): | 01-26-2012 |
20120061258 | SURFACES AND METHODS FOR BIOSENSOR CELLULAR ASSAYS - Disclosed is an apparatus for measuring ligand-induced cell activity as defined herein, the apparatus including: an optical biosensor having a contact surface including a compatibilizer zone, an optional surface modifier zone, and a live cell zone. The disclosure also provides a method of making the apparatus and methods for measuring ligand-induced live cell activity with the apparatus. | 03-15-2012 |
20120064519 | SYSTEM AND METHOD FOR DUAL-DETECTION OF A CELLULAR RESPONSE - A system and method as defined herein for dual-detection of evanescent-wave label-free light and evanescent-wave excited-fluorescent label-emitted light in an optical biosensor. | 03-15-2012 |
20120071351 | LABEL FREE BIOSENSORS AND CELLS - Disclosed are compositions and methods for using label free optical biosensors for performing cell assays. In certain embodiments the assays can be performed in highthough put methods and can be multiplexed. | 03-22-2012 |
20120083428 | Method and Device for Protein Delivery into Cells - Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins. | 04-05-2012 |
20120088695 | Method and Device for Protein Delivery into Cells - Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins. | 04-12-2012 |
20120135423 | Methods for GPCR signaling pathway determination using biosensor-cell assays - A system and method for GPCR signaling pathway analysis and elucidation using a biosensor, a live-cell, and a pathway active compound, as defined herein. | 05-31-2012 |
20120190620 | METHODS TO IDENTIFY TARGETS AND MOLECULES REGULATING PURINOSOMES AND THEIR USES - The present invention discloses methods to identify targets, pathways and molecules regulating purinosomes and their uses for treating pathophysiological disorders associated with purinosomes. Disclosed are methods related to both label-free cellular assays and fluorescence imaging to confirm the regulatory roles of various targets and molecules in purinosome dynamics. Disclosed are methods to classify molecules and the uses of these molecules for different indications. Specifically, the purinosome-disrupting molecules can be used for improved prevention and treatment of cancer development. | 07-26-2012 |
20120289432 | PI3K MODULATORS, RHO KINASE MODULATORS AND METHODS OF IDENTIFYING AND USING SAME - Disclosed are methods to characterize PI3K inhibitors and Rho kinase inhibitors using label-free cellular assays. Disclosed are also methods to characterize a cell whether it has a deregulated PI3K pathway or not. | 11-15-2012 |
20120295965 | FUSED THIOPHENES AS DUAL INHIBITORS OF EGFR/VEGFR AND THEIR USE IN THE TREATMENT OF CANCER - Disclosed are compositions and methods related to identification of modulators of EGFR and VEGFR. | 11-22-2012 |
20120329865 | MOLECULES RELATED hERG ION CHANNELS AND THE USE THEREOF - Disclosed are compounds having structural formula (I, II) or a pharmaceutically acceptable sale, solvate, clathrate, or prodrug thereof, wherein R | 12-27-2012 |
20130005611 | Arrays Of Biological Membranes And Methods And Use Thereof - The present invention overcomes the problems and disadvantages associated with prior art arrays by providing an array comprising a plurality of biological membrane microspots associated with a surface of a substrate that can be produced, used and stored, not in an aqueous environment, but in an environment exposed to air under ambient or controlled humidities. Preferably, the biological membrane microspots comprise a membrane bound protein. Most preferably, the membrane bound protein is a G-protein coupled receptor, an ion channel, a receptor serine/threonine kinase or a receptor tyrosine kinase. | 01-03-2013 |
20130040331 | LABEL-FREE METHODS RELATED TO hERG POTASSIUM ION CHANNELS - Disclosed are methods to classify human ether-à-go-go related gene (hERG) ion channel modulators using label-free biosensors. Disclosed is the use of label-free resonant waveguide grating (RWG) biosensors to reveal the patterns of the DMR signals of hERG modulators across three types of cell lines (a native cell line endogenously expressing hERG, a native cell line without hERG and its engineered cell line stably expressed hERG), as well as the corresponding modulation index of the modulators molecules against a hERG activator acting on a panel of markers/cells, particularly the known hERG activator mallotoxin DMR signals in the two hERG expressing cell lines. | 02-14-2013 |
20130189775 | MODULATION OF THE MAMMALIAN RECEPTOR mTOR TO INHIBIT STEM CELL DIFFERENTIATION INTO NEURONS - The present application relates to compositions and methods for inhibiting the differentiation of isolated embryonic stem cells, induced pluripotent stem cells, parthenogentic stem cells, or isolated embryoid bodies into neuronal progenitor cells or neuron cells comprising a mTOR inhibitor and an effective amount of cell culture growth media. The mTOR inhibitor can be Rapamycin. | 07-25-2013 |
20130210057 | USE OF LABEL-FREE BIOSENSORS TO UNDERSTAND AND IDENTIFY TREATMENT FOR CANCER - The disclosure relates to methods of using dynamic mass redistribution data obtained from cancer cells cultured on waveguide grating biosensors in the presence of agonists and in the presence of chemotherapeutic agents, for predicting effective chemotherapies for the treatment of cancer. | 08-15-2013 |
20140057310 | Label Free Biosensors And Cells - Disclosed are compositions and methods for using label free optical biosensors for performing cell assays. In certain embodiments the assays can be performed in high throughput methods and can be multiplexed. | 02-27-2014 |
20140099653 | DUAL-TARGET BIOSENSOR CELL ASSAYS - A method and apparatus, as defined herein, for use in compound screening, compound profiling, or both assays, for example, against two different cellular targets in, for example, a single cell-type. | 04-10-2014 |