Patent application number | Description | Published |
20090215079 | Method and Devices for the Detection of Microorganisms and/or the Activity Thereof - In a method for detection of microorganisms and/or their activity with biosensors, on a surface of a substrate over portions thereof at least one ligand for binding a receptor or at least one receptor for a ligand or at least one ligand for binding a receptor and at least one receptor for either the one or a further ligand are immobilized chemically, physically, or biologically, wherein physical or physicochemical changes, caused at receptors by binding of ligands that are emitted by microorganisms in the process of quorum sensing are measured. | 08-27-2009 |
20100221817 | Whole-Cell Sensor - The invention relates to whole-cell sensors for monitoring bioavailable nitrogen, phosphorus and sulphur, individually or in at least one combination in a medium, and to the use thereof. The whole-cell sensors consist of genetically modified yeast cells which are immobilised in a xerogel matrix and contain at least one marker gene controlled by a promoter of a gene, the transcription of said gene being significantly increased or reduced in the absence of nitrogen, phosphorus or sulphur, and the yeast cells are at least coupled to a signal detector. | 09-02-2010 |
20100285505 | Device and Method for Detecting a Substance by Means of Particle Plasmon Resonance (PPR) or Particle-Mediated Fluorescence Based on Cell Surface Polarizations - The invention relates to devices and methods for detecting a substance by cell surface polarizations and the detection thereof by means of PPR or particle-mediated fluorescence. The device for detecting a substance by cell surface polarization according to the invention has cells, wherein a gene the expression of which leads to the polarized presentation of a protein on the surface of the cell is placed under the control of a promotor which can be regulated by the substance to be detected, nanoparticles which are functionalized with a molecule which can bind specifically to the surface-exposed protein, and at least one optical measurement device, such that an accumulation of the nanoparticles on the surface of the cells can be detected by particle plasmon resonance or particle-mediated fluorescence. | 11-11-2010 |
Patent application number | Description | Published |
20090215079 | Method and Devices for the Detection of Microorganisms and/or the Activity Thereof - In a method for detection of microorganisms and/or their activity with biosensors, on a surface of a substrate over portions thereof at least one ligand for binding a receptor or at least one receptor for a ligand or at least one ligand for binding a receptor and at least one receptor for either the one or a further ligand are immobilized chemically, physically, or biologically, wherein physical or physicochemical changes, caused at receptors by binding of ligands that are emitted by microorganisms in the process of quorum sensing are measured. | 08-27-2009 |
20100221817 | Whole-Cell Sensor - The invention relates to whole-cell sensors for monitoring bioavailable nitrogen, phosphorus and sulphur, individually or in at least one combination in a medium, and to the use thereof. The whole-cell sensors consist of genetically modified yeast cells which are immobilised in a xerogel matrix and contain at least one marker gene controlled by a promoter of a gene, the transcription of said gene being significantly increased or reduced in the absence of nitrogen, phosphorus or sulphur, and the yeast cells are at least coupled to a signal detector. | 09-02-2010 |
20100285505 | Device and Method for Detecting a Substance by Means of Particle Plasmon Resonance (PPR) or Particle-Mediated Fluorescence Based on Cell Surface Polarizations - The invention relates to devices and methods for detecting a substance by cell surface polarizations and the detection thereof by means of PPR or particle-mediated fluorescence. The device for detecting a substance by cell surface polarization according to the invention has cells, wherein a gene the expression of which leads to the polarized presentation of a protein on the surface of the cell is placed under the control of a promotor which can be regulated by the substance to be detected, nanoparticles which are functionalized with a molecule which can bind specifically to the surface-exposed protein, and at least one optical measurement device, such that an accumulation of the nanoparticles on the surface of the cells can be detected by particle plasmon resonance or particle-mediated fluorescence. | 11-11-2010 |
20110189657 | Device and a Method for the Detection and Amplification of a Signal - The invention relates to a device and a method for the detection and amplification of a primary signal, utilizing an intracellular communication system, and the use thereof for the detection of substances such as phosphorus, sulfur, nitrogen, hormones, metabolic intermediates, fermentation products, and so forth. The device according to the invention for the detection and amplification of a primary signal contains cells of a first type for which a gene, which is responsible for the synthesis of a signal molecule, is under the control of a promoter which is regulated by the primary signal, and cells of a second type for which a specific gene is under the control of a promoter which is regulated by the separated signal molecule, in such a way that the secretion of the signal molecule is induced by a primary signal taken up by a cell of the first type, and the primary signal is amplified by the cells of the second type by the expression of the specific gene under the control of the signal molecule. | 08-04-2011 |
20110256579 | Method for Verifying and/or Identifying Hormonally Effective Substances - The invention relates to a method for verifying and/or identifying hormonally effective substances using the pheromone system of yeast, to yeast cells for carrying out the method, and to the use thereof. The method according to the invention comprises the following steps: a) adding yeast culture medium or buffer to a sample which presumably contains a hormonally effective substance, b) thereafter contacting the sample with haploid yeast cells that possess features 1 through 3: 1. the DNA sequence coded for a heterologous hormone receptor is placed under the control of a constitutive promoter, 2. the DNA sequence coded for a yeast pheromone is placed under the control of a promoter that can be regulated by the hormone receptor, 3. a gene that is coded for a receptor for the yeast pheromone is constitutively expressed, wherein the activation of the hormone receptor by the hormonally effective substance leads to the expression and secretion of the yeast pheromone, and c) the physiological and/or morphological changes of haploid yeast cells are detected. | 10-20-2011 |
20140255712 | Method for coating substrates with at least one monolayer of self-assembling proteins - The invention relates to methods for coating a substrate with at least one monolayer of self-assembling proteins, using stabilized aqueous solutions with self-assembling proteins, and also to substrates obtainable as a result. Methods for stabilizing solutions with self-assembling proteins, and the stabilized solutions obtainable therefrom, are likewise provided by the invention. According to the coating method, at least one monolayer of a self-assembling protein is produced on a substrate by first providing a stabilized aqueous solution which comprises at least one self-assembling protein. To provide the coating solution, protein units aggregated from an aqueous solution of self-assembling proteins are separated off, and addition of a solution of ionic surfactants and/or of a salt-containing and/or alkaline and/or acidic solution to the protein-containing coating solution generates monomers or oligomers of the self-assembling proteins, and stabilizes them, the amount of ionic surfactant added being such that only the surface-active part of each active protein monomer or predominant protein oligomer is enveloped by surfactant particles. A substrate surface is then brought into contact with the stabilized, protein-containing solution, thus producing a protein-containing coating on the substrate. The supernatant solution is removed from the coated substrate and/or the coated substrate is dried. | 09-11-2014 |