Pausch
Mark H. Pausch, Rocky Hill, NJ US
Patent application number | Description | Published |
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20100293118 | METHODS AND SYSTEMS FOR PREDICTING PROTEIN-LIGAND COUPLING SPECIFICITIES - The invention provides methods and systems for predicting or evaluating protein-ligand coupling specificities. A pattern recognition model can be trained by selected sequence segments of training proteins which have a specified ligand coupling specificity. Each selected sequence segment is believed to include amino acid residue(s) that may contribute to the ligand coupling specificity of the corresponding training protein. Sequence segments in a protein of interest can be similarly selected and used to query the trained model to determine if the protein of interest has the same ligand coupling specificity as the training proteins. In one embodiment, the pattern recognition model employed is a hidden Markov model which is trained by concatenated cytosolic domains of GPCRs which have interaction preference to a specified class of G proteins. This trained model can be used to evaluate G protein coupling specificity of orphan GPCRs. | 11-18-2010 |
Mark Henry Pausch, Robbinsville, NJ US
Patent application number | Description | Published |
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20090081764 | Heterologous G protein coupled receptors expressed in yeast, their fusion with G proteins and use thereof in bioassay - The present invention is directed to expression vectors and yeast cells transformed therewith containing a first heterologous nucleotide sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second nucleotide sequence which codes for all or a portion of a G protein αβγ complex. Said heterologous protein is physically expressed in a host cell membrane in proper orientation for both stereoselective binding of ligands, as well as functional interaction with G proteins on the cytoplasmic side of the cell membrane. In some embodiments, a nucleotide sequence encoding a heterologous or chimeric Gα protein is expressed in conjunction with nucleotide sequences from the yeast G protein βγ subunits. A second aspect of the present invention provides expression vectors and yeast cells transformed therewith encoding chimeric yeast/heterologous G protein coupled receptors. A third aspect of the present invention is directed to methods of assaying compounds using such expression constructs and yeast cell expression systems to determine the effects of ligand binding to the heterologous receptors expressed in the systems. | 03-26-2009 |
Tobias Pausch, Tokyo JP
Patent application number | Description | Published |
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20090115177 | Airbag and Airbag Apparatus - The area of panels used in an airbag that inflates so that a recess extends in the vertical direction between a left chamber and a right chamber. The airbag | 05-07-2009 |
20090309341 | Airbag device - An airbag is inflated by gas for deployment to the front of and for restraining an occupant. A tether allows an inner capacity of the airbag to be equal to or smaller than a predetermined value by restricting a distance between a proximal end of the airbag and a surface of the airbag at deployment. A control unit acquires information relating to at least one of an occupant seat position, an occupant position to an instrument panel, an occupant physique, an occupant weight, and a magnitude of a collision impact. A release unit controls capacity and configuration of the airbag by releasing an end of the tether at a side of the proximal end according to the information acquired by the control unit. A vent discharges the gas before activation of the release unit and is closed on activation of the release unit. | 12-17-2009 |
20100019476 | Airbag and airbag apparatus - An airbag includes a vent and a gas discharge limitation member to limit gas discharged from the vent. The limitation member includes at least one invertible portion on a surface on a side opposite the occupant-facing surface of the airbag and capable everting toward the outside of the airbag. The limitation member includes an interlocking member for interlocking movement of the occupant-facing surface in an occupant direction and an opposite direction and for interlocking movement of the invertible portion inward of the airbag and outward of the airbag when expanded. The limitation member includes a lid covering the vent and being pulled by the invertible portion when the invertible portion everts towards the outside of the airbag. The lid closes the vent or opens the vent by a small amount until the lid member is pulled by the invertible portion and opens the vent by at least a large amount. | 01-28-2010 |