Patent application number | Description | Published |
20110224640 | CARTRIDGE AND NEEDLE SYSTEM THEREFOR - The invention refers to a cartridge ( | 09-15-2011 |
20120252852 | OTAMIXABAN FORMULATIONS WITH IMPROVED STABILITY - The invention relates to a pharmaceutical composition comprising methyl (2R,3R)-2-{3-[amino(imino)methyl]benzyl}-3-{[4-(1-oxidopyridin-4-yl)benzoyl]amino}butanoate or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable acidic reacting compound or to an aqueous solution or dispersion of the composition as well as a process for the preparation of the same, methods of using such compositions to treat subjects suffering from conditions which can be ameliorated by the administration of an inhibitor of Factor Xa. | 10-04-2012 |
20120259288 | ASSEMBLY KIT FOR PREPARING OF A PRE-FILLED SYRINGE - The present invention relates to an assembly kit for preparing of a pre-filled syringe and in particular to a method of preparing of such pre-filled syringe assemblies by means of an assembly kit, wherein the assembly kit comprises: a gripping means to be attached to a proximal end section of a cartridge which comprises an axially displaceable bung for sealing of an interior volume of the cartridge being pre-filled with an injectable medicinal product, a needle mount to be attached to a distal end section of the cartridge and providing a mount for a needle assembly to be coupled to the interior volume of the cartridge in a fluid-transferring way, a plunger rod connectable with the bung of the cartridge for applying distally directed thrust to the bung, wherein the cartridge is at least partially embedded in a protecting sleeve. | 10-11-2012 |
20130152510 | PACKAGING SYSTEM FOR MULTI-COMPONENT MEDICAL PRODUCTS - A sterile packaging system for use in the manufacture and sale of combination medical product preferably one that is attachable to an injection system to co-deliver at least two medicaments is disclosed where a tray grid comprises sterile receptacles that are removably connected, sealable and configured for use in an automated drug filling and assembly line. | 06-20-2013 |
Patent application number | Description | Published |
20080277035 | Method and Device for Quenching Flat Steel Products - An apparatus for quenching flat steel products includes a roller conveyor for such flat steel products. The roller conveyor includes upper and lower rollers. At least one nozzle device is arranged above the roller conveyor, and transversely to it, for spraying water onto the flat steel products. At least one suction removal device is arranged above the roller conveyor, and transversely to it, for removing water by suction upstream of an associated upper roller. A method of quenching flat steel products is also provided. | 11-13-2008 |
20100143080 | DEVICE AND METHOD FOR LOADING AND UNLOADING A HEAT TREATMENT FURNACE - The invention relates to a device for loading and unloading a heat treatment furnace and to a corresponding method. Heat treatment furnaces are normally loaded and unloaded by means of a so-called elevator. During unloading, a removed workpiece is usually lowered by means of the elevator into an oil bath, in which it is then removed from the elevator. Oil is then transferred into the furnace chamber via the oil-covered elevator. Such an application of oil is avoided by means of a device comprising a frame rack having a first and a second level, which are disposed parallel above each other, and are connected to each other by means of a plurality of frame braces, wherein the first and the second levels have a plurality of openings and the frame braces have guide means. The device further comprises a plurality of lifting braces that are connected by a lifting mechanism and are supported vertically movably in the guide means, wherein each lifting brace has carriers at a first and a second position, wherein the carriers are aligned on the lifting braces such that they can be moved in the associated levels in or through the openings upon a vertical movement of the lifting braces. The device further comprises a retaining means disposed on a frame brace, which limits the vertical movement of the corresponding lifting brace. | 06-10-2010 |
Patent application number | Description | Published |
20110296965 | METHOD AND DEVICE FOR STRIPPING FIBERS OF A FIBER BUNDLE - The invention relates to a method for stripping fibers of a fiber bundle, wherein each fiber comprises a core and a cladding, wherein the fibers are disposed next to each other on an underlayment such that they extend along a first direction, a cutter extending transverse to the first direction cuts into the claddings and simultaneously all fibers are rolled about the longitudinal axis thereof on the underlayment, so that the cutter generates a cut running in the circumferential direction in each cladding, the fiber bundle is then immersed in a chemical solvent for a prescribed period of time, in order to pre-weaken the bond between the cladding and the core, and the pre-weakened cladding segments are mechanically pulled off of the fiber cores. | 12-08-2011 |
20110302757 | METHOD AND DEVICE FOR STRIPPING FIBRES IN A FIBRE BUNDLE - The invention relates to a method and a device for removing the sheath or for stripping fibres of a fibre bundle. Each fibre comprises a core and a sheath, wherein the fibres are arranged adjacent to each other on a base such as to run in a first direction, a cut is made with two blades running perpendicular to the first direction in a plane from opposing sides such that the blades from a notch in each sheath running in the circumferential direction, the fibre bundle is then dipped in a chemical solvent up to the cuts in the fibres for a given period in order to pre-weaken the connection between the sheath and the core and the pre-weakened sheath sections are mechanically drawn from the fibre cores. | 12-15-2011 |
20110308364 | METHOD AND DEVICE FOR STRIPPING FIBRES IN A FIBRE BUNDLE - The invention relates to a method and a device for removing the sheath or for stripping fibres of a fibre bundle. Each fibre comprises a core and a sheath, wherein the fibres are arranged adjacent to each other on a base such as to run in a first direction, a cut is made with two blades running perpendicular to the first direction in a plane from opposing sides such that the blades from a notch in each sheath running in the circumferential direction, the fibre bundle is then dipped in a chemical solvent up to the cuts in the fibres for a given period in order to pre-weaken the connection between the sheath and the core and d) the pre-weakened sheath sections are mechanically drawn from the fibre cores. | 12-22-2011 |
Patent application number | Description | Published |
20090280189 | System for the liberation of an active principle and its use - A local system for the liberation of an active principle is described which consists of spherical bodies which are composed of polymethyl methacrylate or polymethyl methacrylate co-methyl acrylate and, if necessary, zirconium dioxide and/or barium sulphate and a pharmaceutical active principle, which contains at least one hemostyptically effective compound stable at least up to 120° C., preferably a calcium salt. The local system for the liberation of an active principle is provided as medical product or drug. | 11-12-2009 |
20110015673 | SURGICAL SUTURE MATERIAL WITH AN ANTIMICROBIAL SURFACE AND PROCESS FOR PROVIDING AN ANTIMICROBIAL COATING ON SURGICAL SUTURE MATERIAL - A surgical suture material with an antimicrobial surface is described, the surface exhibiting a coating containing a) at least one fatty acid, b) octenidine dichloride and/or dequalinium chloride and c) optionally oligomeric lactic acid esters. In addition, a process for coating surgical suture material is described which is characterized by the fact that the thread material is wetted with a homogeneous methanolic solution of octenidine dichloride and/or dequalinium chloride and subsequently the methanol is evaporated, a coating forming on the thread surface. | 01-20-2011 |
20110287064 | ANTIBIOTIC COATING - An implant is provided having an antibiotic coating that is inexpensive and easy to manufacture. The antibiotic coating adheres well and in stable manner to the surface of the implant, can be degraded by the body without forming toxic products, prevents the crystallization of the antibiotics in the coating, and ensures a high immediate locally-effective antibiotic concentration at the implant site. A coating solution useful for making the implant and a method for the manufacture of the implant are also described. | 11-24-2011 |
20120164309 | COATING METHOD AND COATING DEVICE - A device for coating at least regions of a medical implant includes an elastically deformable transfer means having a liquid that contains at least one pharmaceutically active substance. The liquid can be transferred to the medical implant when the transfer means is contacted with a surface of the medical implant. | 06-28-2012 |
20120164312 | COATING METHOD AND COATING DEVICE - A method for coating at least regions of a medical implant includes providing a medical implant having a surface to be coated, and immersing the surface of the medical implant into a liquid including at least one pharmaceutically active substance, whereby the liquid is transferred to the surface of the medical implant due to the immersing. The method also includes pulling the surface of the medical implant out of the liquid, whereby part of the liquid remains adhering to the surface of the medical implant. | 06-28-2012 |
Patent application number | Description | Published |
20110012790 | MULTI-SLOT ANTENNA AND MOBILE DEVICE - A mobile communications device having a patch antenna which has defined therein at least two slots each having two or more parts. The at least two slots may include an L-shaped slot and a C-shaped slot, wherein the slots can be open or closed. The L-shaped slot may be an open-slot projecting into the patch antenna from the edge. Ground and signal connections may be at the edge of the patch on either side of the L-shaped slot. The C-shaped slot may be nested within the L-shaped slot. | 01-20-2011 |
20120088560 | MOBILE WIRELESS COMMUNICATIONS DEVICE INCLUDING SPACED APART ELONGATE MEMBERS FOR REDUCING SAR AND RELATED METHODS - A mobile wireless communications device may include a portable housing and wireless transceiver circuitry carried by the portable housing. The mobile wireless communications device may also include an antenna carried by the portable housing and coupled to the wireless transceiver circuitry, and a light source carried by the portable housing. The device may further include a keyboard assembly carried by the portable housing including a light guide coupled to the light source and having input key receiving openings therein, and a mask carried by the light guide and having input key receiving openings. The mask may include spaced apart electrically conductive elongate members positioned relative to the antenna to reduce a Specific Absorption Rate value for the mobile wireless communications device. | 04-12-2012 |
20120244919 | MOBILE WIRELESS COMMUNICATIONS DEVICE INCLUDING SPACED APART ELONGATE MEMBERS FOR REDUCING SAR AND RELATED METHODS - A mobile wireless communications device may include a portable housing and wireless transceiver circuitry carried by the portable housing. The mobile wireless communications device may also include an antenna carried by the portable housing and coupled to the wireless transceiver circuitry, and a light source carried by the portable housing. The device may further include a keyboard assembly carried by the portable housing including a light guide coupled to the light source and having input key receiving openings therein, and a mask carried by the light guide and having input key receiving openings. The mask may include spaced apart electrically conductive elongate members positioned relative to the antenna to reduce a Specific Absorption Rate value for the mobile wireless communications device. | 09-27-2012 |
20130194136 | MOBILE WIRELESS COMMUNICATIONS DEVICE WITH MULTIPLE-BAND ANTENNA AND RELATED METHODS - A mobile wireless communications device may include a housing, a wireless transceiver carried by the housing, and a multiple-band antenna carried by the housing and coupled to the wireless transceiver. The multiple-band antenna may include a first radiator comprising a radiator element and a parasitic element adjacent to the radiator element. The parasitic element may be selectively switchable between floating and grounded states. The multiple-band antenna may include a second radiator insulated from the first radiator. | 08-01-2013 |
Patent application number | Description | Published |
20110012790 | MULTI-SLOT ANTENNA AND MOBILE DEVICE - A mobile communications device having a patch antenna which has defined therein at least two slots each having two or more parts. The at least two slots may include an L-shaped slot and a C-shaped slot, wherein the slots can be open or closed. The L-shaped slot may be an open-slot projecting into the patch antenna from the edge. Ground and signal connections may be at the edge of the patch on either side of the L-shaped slot. The C-shaped slot may be nested within the L-shaped slot. | 01-20-2011 |
20130194136 | MOBILE WIRELESS COMMUNICATIONS DEVICE WITH MULTIPLE-BAND ANTENNA AND RELATED METHODS - A mobile wireless communications device may include a housing, a wireless transceiver carried by the housing, and a multiple-band antenna carried by the housing and coupled to the wireless transceiver. The multiple-band antenna may include a first radiator comprising a radiator element and a parasitic element adjacent to the radiator element. The parasitic element may be selectively switchable between floating and grounded states. The multiple-band antenna may include a second radiator insulated from the first radiator. | 08-01-2013 |
20140015718 | TRI-BAND ANTENNA FOR NONCELLULAR WIRELESS APPLICATIONS - A tri-band antenna for noncellular wireless applications is provided. The antenna comprises: a first radiating arm for generating a first resonance in a first frequency band, the first radiating arm further enabled for connection to an antenna tuning circuit; the first radiating arm comprising a capacitive coupling structure; a coupling arm separated by a gap from the first radiating arm; a second radiating arm for generating a second resonance in a second frequency band lower than the first frequency band, the second radiating arm connected to the coupling arm such that the second radiating arm is capacitively coupled to the first radiating arm; and a third radiating arm for generating a third resonance in a third frequency band lower than the second frequency band, the third radiating arm connected to the coupling arm such that the third radiating arm is capacitively coupled to the first radiating arm. | 01-16-2014 |
20140015720 | TRI-BAND ANTENNA FOR NONCELLULAR WIRELESS APPLICATIONS - A tri-band antenna for noncellular wireless applications is provided. The antenna comprises: a first radiating arm for generating a first resonance in a first frequency band, the first radiating arm further enabled for connection to an antenna tuning circuit; the first radiating arm comprising a capacitive coupling structure; a coupling arm separated by a gap from the first radiating arm; a second radiating arm for generating a second resonance in a second frequency band lower than the first frequency band, the second radiating arm connected to the coupling arm such that the second radiating arm is capacitively coupled to the first radiating arm; and a third radiating arm for generating a third resonance in a third frequency band lower than the second frequency band, the third radiating arm connected to the coupling arm such that the third radiating arm is capacitively coupled to the first radiating arm. | 01-16-2014 |
Patent application number | Description | Published |
20100242127 | METHODS TO IDENTIFY MODULATORS OF B-RAF PROTEIN KINASE AND THEIR USE FOR THE TREATMENT OF ANXIETY AND DEPRESSION - The present invention relates to a method for identifying a compound capable of modulating an anxiety or depression disorder comprising the steps of: (a) contacting a composition comprising a B-Raf protein or a B-Raf gene in expressible form or a transcript thereof with a compound under conditions that allow for an interaction of the B-Raf protein or the B-Raf gene or a transcript thereof and the compound; and (b) measuring whether said interaction, if any, results in (i) a change of B-Raf kinase activity compared to B-Raf kinase activity in the absence of said compound; (ii) a modulation of the expression of the B-Raf gene compared to B-Raf gene expression in the absence of said compound; or (iii) the formation of a complex between the compound and the B-Raf protein, wherein such a change in activity, modulation of expression or the formation of a complex is indicative of the compound being a modulator of an anxiety or depression disorder. Further, the invention relates to a method for treating an anxiety or depression disorder in an individual comprising administering to the individual an effective amount of a compound inhibiting B-Raf kinase activity or gene expression and to a use of a compound that inhibits B-Raf kinase activity or gene expression in the manufacture of a pharmaceutical composition for treating an anxiety or depression disorder. Moreover, the invention relates to a method of diagnosing a B-Raf associated anxiety or depression disorder and to a genetically engineered mouse. Finally, the invention also relates to a method of identifying another gene contributing to the pathophysiology of an anxiety or depression disorder apart from B-Raf. | 09-23-2010 |
20100299771 | MEANS AND METHODS FOR shRNA MEDIATED CONDITIONAL KNOCKDOWN OF GENES - The present invention relates to a combination of DNA segments comprising: (a) a first segment comprising in 5′ to 3′ or 3′ to 5′ order: (aa) a promoter; (ab) a first DNA sequence comprising: (i) a DNA sequence giving rise upon transcription to the sense strand of an shRNA molecule; (ii) a transcriptional stop element which is flanked by a first type of recombinase recognition sequences; and (iii) a DNA sequence giving rise upon transcription to the antisense strand of an shRNA molecule; (b) a second segment comprising in 5′ to 3′ or 3′ to 5′ order: (ba) a promoter; (bb) a second DNA sequence comprising: (i) a DNA sequence giving rise upon transcription to the sense strand of an shRNA molecule; (ii) a transcriptional stop element which is flanked by a second type of recombinase recognition sequences; and (iii) a DNA sequence giving rise upon transcription to the antisense strand of an shRNA molecule; wherein (i) said first type of recombinase recognition sequences are recognized and recombined by a recombinase but not recombined with said second type of recombinase recognition sequences; (ii) said second type of recombinase recognition sequences are recognized and recombined by the recombinase of (i) but not recombined with said first type of recombinase recognition sequences; and (iii) said DNA sequence of (ab) and (bb) is expressed under the control of said promoters of (aa) and (ba) upon removal of said transcriptional stop elements of (ab) and (bb) by the activity of a recombinase, resulting in transcription of said shRNA molecule in a cell. Further, the invention relates to a genetically engineered non-human animal and a method to produce said transgenic non-human animal. Also, the invention relates to a cell genetically engineered with the DNA molecule of the invention and a method of simultaneously knocking down two genes in a cell. Furthermore, envisaged is a method of identifying a combination of two target genes as a potential drug target and the use of the DNA molecule of the invention for the preparation of a composition for gene therapy. | 11-25-2010 |
20110061118 | VECTORS AND METHODS FOR GENERATING VECTOR-FREE INDUCED PLURIPOTENT STEM (IPS) CELLS USING SITE-SPECIFIC RECOMBINATION - The present invention relates to a DNA molecule comprising: (a) a first DNA sequence comprising: (aa) a coding sequence giving rise upon transcription to a factor that contributes to the reprogramming of a somatic cell into an induced pluripotent stem (iPS) cell; (ab) a promoter mediating the transcription of said coding sequence; and (ac) two sequence motifs that mediate excision of (aa) and/or (ab) from the DNA molecule, wherein one sequence motif is positioned 5′ and the other sequence motif is positioned 3′ of the sequence to be excised; (b) a second DNA sequence comprising a sequence motif that mediates site-specific integration of (a) into another DNA molecule. Further, the invention relates to DNA molecule comprising: (a) a first DNA sequence comprising: (aa) a coding sequence giving rise upon transcription to a factor that contributes to the reprogramming of a somatic cell into an induced pluripotent stem cell; and (ab) a promoter mediating the transcription of said coding sequence; (b) a second DNA sequence comprising: (ba) a sequence motif that mediates extrachromosomal self-replication of the DNA-molecule; and (bb) two sequence motifs that mediate excision of at least said sequence motif of (ba) from the second DNA sequence (b), wherein one sequence motif is located 5′ of (ba) and the other sequence motif 3′ of (ba). Also, the invention relates to a vector comprising the DNA molecule of the invention, a method for assembly of said vector and a somatic cell comprising said DNA molecule or said vector of the invention. Furthermore, the invention relates to methods to generate an induced pluripotent stem (iPS) cell, an induced pluripotent stem cell obtainable by said methods, to a kit comprising the DNA molecule of the invention, to a cell line or cell culture collection comprising the induced pluripotent stem cell of the invention, to the use of said cell or cell line as a research tool, to a method to generate a transgenic non-human animal and to a non-human animal generated by said method. Finally, the invention relates to a composition for gene therapy, regenerative medicine, cell therapy or drug screening. | 03-10-2011 |
20130212725 | FUSION PROTEINS COMPRISING A DNA-BINDING DOMAIN OF A TAL EFFECTOR PROTEIN AND A NON-SPECIFIC CLEAVAGE DOMAIN OF A RESTRICTION NUCLEASE AND THEIR USE - The present invention relates to a method of modifying a target sequence in the genome of a eukaryotic cell, the method comprising the step: (a) introducing into the cell a fusion protein comprising a DNA-binding domain of a Tal effector protein and a non-specific cleavage domain of a restriction nuclease or a nucleic acid molecule encoding the fusion protein in expressible form, wherein the fusion protein specifically binds within the target sequence and introduces a double strand break within the target sequence. The present invention further relates to the method of the invention, wherein the modification of the target sequence is by homologous recombination with a donor nucleic acid sequence further comprising the step: (b) introducing a nucleic acid molecule into the cell, wherein the nucleic acid molecule comprises the donor nucleic acid sequence and regions homologous to the target sequence. The present invention also relates to a method of producing a non-human mammal or vertebrate carrying a modified target sequence in its genome. Furthermore, the present invention relates to a fusion protein comprising a DNA-binding domain of a Tal effector protein and a non-specific cleavage domain of a restriction nuclease. | 08-15-2013 |
20140298505 | PROTEIN HAVING NUCLEASE ACTIVITY, FUSION PROTEINS AND USES THEREOF - The present invention relates to a nucleic acid molecule encoding (I) a polypeptide having the activity of an endonuclease, which is (a) a nucleic acid molecule encoding a polypeptide comprising or consisting of the amino acid sequence of SEQ ID NO: 1; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 2; (c) a nucleic acid molecule encoding an endonuclease, the amino acid sequence of which is at least 70% identical to the amino acid sequence of SEQ ID NO: 1; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 50% identical to the nucleotide sequence of SEQ ID NO: 2; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (e) wherein T is replaced by U; (II) a fragment of the polypeptide of (I) having the activity of an endonuclease. Also, the present invention relates to a vector comprising the nucleic acid molecule and a protein encoded by said nucleic acid molecule. Further, the invention relates to a method of modifying the genome of a eukaryotic cell and a method of producing a non-human vertebrate or mammal. | 10-02-2014 |
20140304847 | RECOMBINATION EFFICIENCY BY INHIBITION OF NHEJ DNA REPAIR - The present invention relates to a method for modifying a target sequence in the genome of a mammalian cell, the method comprising the step of introducing into a mammalian cell: a. one or more compounds that introduce double-strand breaks in said target sequence; b. one or more DNA molecules comprising a donor DNA sequence to be incorporated by homologous recombination into the genomic DNA of said mammalian cell within said target sequence, wherein said donor DNA sequence is flanked upstream by a first flanking element and downstream by a second flanking element, wherein said first and second flanking element are different and wherein each of said first and second flanking sequence are homologous to a continuous DNA sequence on either side of the double-strand break introduced by said one or more compounds of a. within said target sequence in the genome of said mammalian cell; and c. one or more compounds that decrease the activity of the non-homologous end joining (NHEJ) DNA repair complex in said mammalian cell. Further, the invention relates to a method of producing a non-human mammal carrying a modified target sequence in its genome. | 10-09-2014 |
Patent application number | Description | Published |
20100242127 | METHODS TO IDENTIFY MODULATORS OF B-RAF PROTEIN KINASE AND THEIR USE FOR THE TREATMENT OF ANXIETY AND DEPRESSION - The present invention relates to a method for identifying a compound capable of modulating an anxiety or depression disorder comprising the steps of: (a) contacting a composition comprising a B-Raf protein or a B-Raf gene in expressible form or a transcript thereof with a compound under conditions that allow for an interaction of the B-Raf protein or the B-Raf gene or a transcript thereof and the compound; and (b) measuring whether said interaction, if any, results in (i) a change of B-Raf kinase activity compared to B-Raf kinase activity in the absence of said compound; (ii) a modulation of the expression of the B-Raf gene compared to B-Raf gene expression in the absence of said compound; or (iii) the formation of a complex between the compound and the B-Raf protein, wherein such a change in activity, modulation of expression or the formation of a complex is indicative of the compound being a modulator of an anxiety or depression disorder. Further, the invention relates to a method for treating an anxiety or depression disorder in an individual comprising administering to the individual an effective amount of a compound inhibiting B-Raf kinase activity or gene expression and to a use of a compound that inhibits B-Raf kinase activity or gene expression in the manufacture of a pharmaceutical composition for treating an anxiety or depression disorder. Moreover, the invention relates to a method of diagnosing a B-Raf associated anxiety or depression disorder and to a genetically engineered mouse. Finally, the invention also relates to a method of identifying another gene contributing to the pathophysiology of an anxiety or depression disorder apart from B-Raf. | 09-23-2010 |
20100299771 | MEANS AND METHODS FOR shRNA MEDIATED CONDITIONAL KNOCKDOWN OF GENES - The present invention relates to a combination of DNA segments comprising: (a) a first segment comprising in 5′ to 3′ or 3′ to 5′ order: (aa) a promoter; (ab) a first DNA sequence comprising: (i) a DNA sequence giving rise upon transcription to the sense strand of an shRNA molecule; (ii) a transcriptional stop element which is flanked by a first type of recombinase recognition sequences; and (iii) a DNA sequence giving rise upon transcription to the antisense strand of an shRNA molecule; (b) a second segment comprising in 5′ to 3′ or 3′ to 5′ order: (ba) a promoter; (bb) a second DNA sequence comprising: (i) a DNA sequence giving rise upon transcription to the sense strand of an shRNA molecule; (ii) a transcriptional stop element which is flanked by a second type of recombinase recognition sequences; and (iii) a DNA sequence giving rise upon transcription to the antisense strand of an shRNA molecule; wherein (i) said first type of recombinase recognition sequences are recognized and recombined by a recombinase but not recombined with said second type of recombinase recognition sequences; (ii) said second type of recombinase recognition sequences are recognized and recombined by the recombinase of (i) but not recombined with said first type of recombinase recognition sequences; and (iii) said DNA sequence of (ab) and (bb) is expressed under the control of said promoters of (aa) and (ba) upon removal of said transcriptional stop elements of (ab) and (bb) by the activity of a recombinase, resulting in transcription of said shRNA molecule in a cell. Further, the invention relates to a genetically engineered non-human animal and a method to produce said transgenic non-human animal. Also, the invention relates to a cell genetically engineered with the DNA molecule of the invention and a method of simultaneously knocking down two genes in a cell. Furthermore, envisaged is a method of identifying a combination of two target genes as a potential drug target and the use of the DNA molecule of the invention for the preparation of a composition for gene therapy. | 11-25-2010 |
20110061118 | VECTORS AND METHODS FOR GENERATING VECTOR-FREE INDUCED PLURIPOTENT STEM (IPS) CELLS USING SITE-SPECIFIC RECOMBINATION - The present invention relates to a DNA molecule comprising: (a) a first DNA sequence comprising: (aa) a coding sequence giving rise upon transcription to a factor that contributes to the reprogramming of a somatic cell into an induced pluripotent stem (iPS) cell; (ab) a promoter mediating the transcription of said coding sequence; and (ac) two sequence motifs that mediate excision of (aa) and/or (ab) from the DNA molecule, wherein one sequence motif is positioned 5′ and the other sequence motif is positioned 3′ of the sequence to be excised; (b) a second DNA sequence comprising a sequence motif that mediates site-specific integration of (a) into another DNA molecule. Further, the invention relates to DNA molecule comprising: (a) a first DNA sequence comprising: (aa) a coding sequence giving rise upon transcription to a factor that contributes to the reprogramming of a somatic cell into an induced pluripotent stem cell; and (ab) a promoter mediating the transcription of said coding sequence; (b) a second DNA sequence comprising: (ba) a sequence motif that mediates extrachromosomal self-replication of the DNA-molecule; and (bb) two sequence motifs that mediate excision of at least said sequence motif of (ba) from the second DNA sequence (b), wherein one sequence motif is located 5′ of (ba) and the other sequence motif 3′ of (ba). Also, the invention relates to a vector comprising the DNA molecule of the invention, a method for assembly of said vector and a somatic cell comprising said DNA molecule or said vector of the invention. Furthermore, the invention relates to methods to generate an induced pluripotent stem (iPS) cell, an induced pluripotent stem cell obtainable by said methods, to a kit comprising the DNA molecule of the invention, to a cell line or cell culture collection comprising the induced pluripotent stem cell of the invention, to the use of said cell or cell line as a research tool, to a method to generate a transgenic non-human animal and to a non-human animal generated by said method. Finally, the invention relates to a composition for gene therapy, regenerative medicine, cell therapy or drug screening. | 03-10-2011 |
20120276537 | HOMOLOGOUS RECOMBINATION IN THE OOCYTE - The present invention relates to a method of modifying a target sequence in the genome of a mammalian or avian oocyte by homologous recombination with a donor nucleic acid sequence, the method comprising the steps (a) introducing into the oocyte a zinc finger nuclease or a nucleic acid molecule encoding the zinc finger nuclease in expressible form, wherein the zinc finger nuclease specifically binds within the target sequence and introduces a double strand break within the target sequence; and (b) introducing a nucleic acid molecule into the oocyte, wherein the nucleic acid molecule comprises the donor nucleic acid sequence and regions homologous to the target sequence. The present invention further relates to a method of producing a non-human mammal or an avian carrying a modified target sequence in its genome. | 11-01-2012 |
20130212725 | FUSION PROTEINS COMPRISING A DNA-BINDING DOMAIN OF A TAL EFFECTOR PROTEIN AND A NON-SPECIFIC CLEAVAGE DOMAIN OF A RESTRICTION NUCLEASE AND THEIR USE - The present invention relates to a method of modifying a target sequence in the genome of a eukaryotic cell, the method comprising the step: (a) introducing into the cell a fusion protein comprising a DNA-binding domain of a Tal effector protein and a non-specific cleavage domain of a restriction nuclease or a nucleic acid molecule encoding the fusion protein in expressible form, wherein the fusion protein specifically binds within the target sequence and introduces a double strand break within the target sequence. The present invention further relates to the method of the invention, wherein the modification of the target sequence is by homologous recombination with a donor nucleic acid sequence further comprising the step: (b) introducing a nucleic acid molecule into the cell, wherein the nucleic acid molecule comprises the donor nucleic acid sequence and regions homologous to the target sequence. The present invention also relates to a method of producing a non-human mammal or vertebrate carrying a modified target sequence in its genome. Furthermore, the present invention relates to a fusion protein comprising a DNA-binding domain of a Tal effector protein and a non-specific cleavage domain of a restriction nuclease. | 08-15-2013 |