Patent application number | Description | Published |
20100159529 | DRY COMPOSITION OF REACTION COMPOUNDS WITH STABILIZED POLMERASE - The present invention provides methods to obtain dry compositions of reaction compounds that maintain the biological activity of the compounds upon re-solubilization after a certain storage time. Preferably, the dry composition comprises a polymerase, and the dry composition is usable for polymerase chain reaction (PCR) amplification after re-solubilization. | 06-24-2010 |
20100273235 | STABILIZED AQUEOUS ALPHA-GALACTOSIDASE COMPOSITION AND METHODS RELATING THERETO - The present invention provides an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase. The present invention further provides a method of stabilizing an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase, and a method of preparing a purified aqueous composition comprising the protein with enzymatic activity of alpha-galactosidase. | 10-28-2010 |
20110250598 | DETERGENT FREE POLYMERASES - The present invention relates to a formulation of a thermostable DNA polymerase which is completely free of detergents and its particular use in real time polymerase chain reaction (PCR). Such a formulation may be obtained if the selected purification method does not require the addition of a detergent at any purification step. | 10-13-2011 |
20110256589 | T7 RNA POLYMERASE VARIANTS WITH ENHANCED THERMOSTABILITY - The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous. | 10-20-2011 |
20120252071 | T7 RNA POLYMERASE VARIANTS WITH CYSTEINE-SERINE SUBSTITUTIONS - The present disclosure provide novel variants of T7 RNA polymerase. Embodiments of T7 variants, according to the instant invention, include a Cysteine-Serine substitution on position 723 of the amino acid sequence of the T7 polypeptide. Embodiments of T7 variants according to the instant invention have a DNA-dependent RNA polymerase enzymatic activity and a reduced tendency to form intramolecular homodimers by way of oxidizing thiol groups. The amino acid substitutions within the T7 variants disclosed herein impact minimally, if at all, the RNA polymerase activity of the T7 polypeptide. Further, the mutations of the disclosed embodiments may optionally be combined with mutations which provide enhanced thermostability compared to the wild-type reference. | 10-04-2012 |
20140134633 | DETERGENT FREE POLYMERASES - The present invention relates to a formulation of a thermostable DNA polymerase which is completely free of detergents and its particular use in real time polymerase chain reaction (PCR). Such a formulation may be obtained if the selected purification method does not require the addition of a detergent at any purification step. | 05-15-2014 |
20140162267 | DRY COMPOSITION OF REACTION COMPOUNDS WITH STABILIZED POLYMERASE - The present invention provides methods to obtain dry compositions of reaction compounds that maintain the biological activity of the compounds upon re-solubilization after a certain storage time. Preferably, the dry composition comprises a polymerase, and the dry composition is usable for polymerase chain reaction (PCR) amplification after re-solubilization. | 06-12-2014 |
20160032260 | T7 RNA POLYMERASE VARIANTS WITH ENHANCED THERMOSTABILITY - The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous. | 02-04-2016 |
20160032261 | T7 RNA POLYMERASE VARIANTS WITH ENHANCED THERMOSTABILITY - The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous. | 02-04-2016 |