Patent application number | Description | Published |
20080213894 | Disposable Tubing Set for Use with a Cell Expansion Apparatus and Method for Sterile Sampling - A disposable apparatus for cell expansion, having at least one bioreactor. The bioreactor has a cellular growth area and a supply area, the cellular growth area being separated from said supply area by a membrane. A fluid recirculation path in fluid communication with the cellular growth area allows for hermetically removing a sample containing cellular matter. This may comprise an elongated tube, or a plurality of parallel tube segments. The parallel tube segments have inflow ends and outflow ends, and the inflow ends are joined at a first common juncture and the outflow ends are joined at a second common juncture. The common junctures may comprise valves. | 09-04-2008 |
20080220522 | Methods to Control Cell Movement in Hollow Fiber Bioreactors - This invention is directed toward methods of moving cells throughout a hollow fiber bioreactor using fluid flow. | 09-11-2008 |
20080220523 | CELL EXPANSION SYSTEM AND METHODS OF USE - Cell expansion systems and methods of use are provided. The cell expansion systems generally include a hollow fiber cell growth chamber, and first and second circulation loops (intracapillary loops and extracapillary loops) associated with the interior of the hollow fibers and exterior of the hollow fibers, respectively. Detachable flow circuits and methods of expanding cells are also provided. | 09-11-2008 |
20080227190 | Cell Expansion Apparatus with Plate Bioreactor - A disposable apparatus for cell expansion, having at least one bioreactor. The bioreactor has a cellular growth area and a supply area, the cellular growth area being adjacent a plate and separated from said supply area by a membrane. The bioreactor comprises a plurality of plates. The plates have a roughened surface or a corrugated surface. At least a sheet of membrane separates adjacent plates. Two sheets between adjacent plates may be separated by a mesh. | 09-18-2008 |
20080248572 | Bioreactor Surfaces - The present invention relates to treatment of polymeric bioreactor surfaces, to promote the proliferation of adherent cells. | 10-09-2008 |
20080254533 | Cell Expansion System and Methods of Use - Cell expansion systems are provided. The cell expansion systems generally include a hollow fiber cell growth chamber, and first and second fluid flow paths associated with the interior of the hollow fibers and exterior of the hollow fibers, respectively. The hollow fibers have a hydrophilic interior surface and a hydrophobic exterior surface. Detachable flow circuits are also provided. | 10-16-2008 |
20100042260 | Predictor of When to Harvest Cells Grown in a Bioreactor - This invention relates to a cell expansion system and to a method of determining when to harvest cells from the cell expansion system. | 02-18-2010 |
20100144037 | Disposable Tubing Set for Use with a Cell Expansion Apparatus and Method for Sterile Sampling - A disposable apparatus for cell expansion, having at least one bioreactor. The bioreactor has a cellular growth area and a supply area, the cellular growth area being separated from said supply area by a membrane. A fluid recirculation path in fluid communication with the cellular growth area allows for hermetically removing a sample containing cellular matter. This may comprise an elongated tube, or a plurality of parallel tube segments. The parallel tube segments have inflow ends and outflow ends, and the inflow ends are joined at a first common juncture and the outflow ends are joined at a second common juncture. The common junctures may comprise valves. | 06-10-2010 |
20110263019 | Method of Reseeding Adherent Cells Grown in a Hollow Fiber Bioreactor System - This invention is directed to methods of directly reseeding harvested adherent cells grown in a hollow fiber bioreactor. Also disclosed is a novel harvest media for use in directly reseeding adherent cells into a hollow fiber bioreactor. | 10-27-2011 |
20110275056 | Method and Apparatus for Controlling a Cell Expansion Apparatus - A method for controlling cell expansion comprising conducting a fluid containing cellular matter into a cellular growth area; providing oxygenated fluid to said cellular growth area to maintain conditions conducive for cell growth in said cellular growth area; monitoring inflow and outflow conditions; determining rates of change (first derivative) for selected conditions and calculating cell numbers therefrom; determining a rate of change of a rate of change (second derivative) for the selected conditions and calculating a predicted process time; and terminating the growth process when adequate cell numbers have been produced and an apparatus for performing the method. | 11-10-2011 |
20120086657 | Configurable Methods and Systems of Growing and Harvesting Cells in a Hollow Fiber Bioreactor System - Embodiments described herein generally relate to methods and systems for configuring settings of a cell expansion system including a bioreactor. Through a user interface, a user may configure display settings, system settings, and settings associated with protocols for loading, growing and/or harvesting cells. In configuring settings for protocols and associated processes, a diagram view or window of the cell expansion system is displayed in embodiments. The diagram view displays the process settings as graphical user interface elements. Settings available for configuration are enabled for selection in the diagram view. The diagram view allows the user to visualize the settings available for task configuration and to configure enabled settings. Configured settings are stored and capable of retrieval for subsequent execution or modification of the applicable protocol. | 04-12-2012 |
20120088224 | Methods and Systems of Growing and Harvesting Cells in a Hollow Fiber Bioreactor System with Control Conditions - Embodiments described herein generally relate to methods and systems for using an air removal chamber as a control for a process in a cell expansion system. The air removal chamber may be mounted on a fluid conveyance assembly for use with the system. Fluid is pumped into a fluid containment chamber of the air removal chamber, in which the level of fluid in the fluid containment chamber may be monitored through the use of one or more sensors. The sensors are capable of detecting air, a lack of fluid, fluid, and/or a gas/fluid interface, e.g., an air/fluid interface, at measuring positions within the air removal chamber. Protocols for use with the system may include one or more stop conditions. In an embodiment, the stopping of a process is automated based on the detection of air, a lack of fluid, and/or a gas/fluid interface in the air removal chamber. | 04-12-2012 |
20120089930 | Customizable Methods and Systems of Growing and Harvesting Cells in a Hollow Fiber Bioreactor System - Embodiments described herein generally relate to methods and systems for customizing protocols for use with a cell expansion system. Through a user interface, a user may create a custom task for loading, growing and/or harvesting cells. A custom task may comprise one or more steps, in which a user may add or omit steps, as desired. Data may be entered for settings associated with a custom task, in which embodiments provide for such data to be entered each time the custom task is performed. In another embodiment, the settings for a custom task may be configured, in which such settings may be stored and retrieved upon selection of the custom task. Customization and configuration of a custom task may occur using a diagram view of the cell expansion system, in which process settings are associated with graphical user interface elements. | 04-12-2012 |
20120295352 | Systems and Methods for Expanding High Density Non-Adherent Cells - Embodiments described herein generally relate to systems and methods for promoting the expansion of high density non-adherent cells through the use of a cell growth chamber, a mass transfer device, and a fluid circulation loop. Improved cell growth is achieved in the cell growth chamber by using a chamber having a particular orientation and shape, e.g., conical, to create a media-rich reservoir for growing cells. By placing the chamber in a vertical position, the force of media flow along the chamber walls is substantially equal and opposite to the gravitational force on the cells. The interaction of these forces maintains the non-adherent cells in suspension. The use of the cell growth chamber in conjunction with the mass transfer device and fluid circulation loop(s) creates efficiencies by relying on the cumulative and combined features of the devices. | 11-22-2012 |
20130102070 | Cell Expansion System and Methods of Use - Cell expansion systems and methods of use are provided. The cell expansion systems generally include a hollow fiber cell growth chamber, and first and second circulation loops (intracapillary loops and extracapillary loops) associated with the interior of the hollow fibers and exterior of the hollow fibers, respectively. Detachable flow circuits and methods of expanding cells are also provided. | 04-25-2013 |
20140178995 | Method of Reseeding Adherent Cells Grown in a Hollow Fiber Bioreactor System - This invention is directed to methods of directly reseeding harvested adherent cells grown in a hollow fiber bioreactor. Also disclosed is a novel harvest media for use in directly reseeding adherent cells into a hollow fiber bioreactor. | 06-26-2014 |
20150024492 | Cell Expansion System and Methods of Use - Cell expansion systems and methods of use are provided. The cell expansion systems generally include a hollow fiber cell growth chamber, and first and second circulation loops (intracapillary loops and extracapillary loops) associated with the interior of the hollow fibers and exterior of the hollow fibers, respectively. Detachable flow circuits and methods of expanding cells are also provided. | 01-22-2015 |
20150079664 | Methods and Systems of Growing and Harvesting Cells in a Hollow Fiber Bioreactor System with Control Conditions - Embodiments described herein generally relate to methods and systems for using an air removal chamber as a control for a process in a cell expansion system. The air removal chamber may be mounted on a fluid conveyance assembly for use with the system. Fluid is pumped into a fluid containment chamber of the air removal chamber, in which the level of fluid in the fluid containment chamber may be monitored through the use of one or more sensors. The sensors are capable of detecting air, a lack of fluid, fluid, and/or a gas/fluid interface, e.g., an air/fluid interface, at measuring positions within the air removal chamber. Protocols for use with the system may include one or more stop conditions. In an embodiment, the stopping of a process is automated based on the detection of air, a lack of fluid, and/or a gas/fluid interface in the air removal chamber. | 03-19-2015 |