Patent application number | Description | Published |
20110193187 | ELECTRODE MEMBER FOR SPECIFIC DETECTION OF ANALYTE USING PHOTOCURRENT - Disclosed is an electrode member for specific detection of an analyte using a photocurrent. The electrode member has at least a conductive substrate and an electron-accepting substance provided on said conductive substrate. The aforementioned electron-accepting substance consists at least of a first substance layer that is made of a semiconductor and a second substance that is made of a semiconductor of a kind different from that of the aforementioned semiconductor, a metal or a metal oxide, and is carried on the surface of said first substance layer. With the electrode member, improved detection sensitivity for the test substance and improved measurement precision can be achieved with specific detection of an analyte using a photocurrent. | 08-11-2011 |
20110220517 | METHOD FOR SPECIFICALLY DETECTING TEST SUBSTANCE USING PHOTOCURRENT, SENSOR UNIT USED THEREFOR, AND MEASURING DEVICE - This invention provides a method for detecting an analyte, which, in specific detection of an analyte using photocurrent that is generated by photoexcitation of a sensitizing dye, can significantly simplify the structure and a detection procedure of a sensor unit and a device using the sensor unit and can realize detection with high accuracy, and also provides a sensor unit and a device using the sensor unit. According to the method, an analyte-containing sample solution and a sensitizing dye are brought into contact with the surface of a working electrode to immobilize the sensitizing dye onto the working electrode through specific binding. Subsequently, an electrolyte is supplied in situ while allowing the sample solution to be held without being removed to bring the working electrode and a counter electrode into contact with the electrolyte. The working electrode is irradiated with light to photoexcite the sensitizing dye and thus to detect photocurrent that flows across the working electrode and the counter electrode. | 09-15-2011 |
20120100627 | MEASUREMENT DEVICE USED FOR SPECIFICALLY DETECTING SUBSTANCE TO BE EXAMINED USING PHOTOCURRENT, SENSOR UNIT USED FOR SAME, AND METHOD FOR SPECIFICALLY DETECTING SUBSTANCE TO BE EXAMINED USING PHOTOCURRENT - In utilizing photocurrent generated in the photoexcitation of a dye in specific detection of an analyte, highly accurate detection can be realized by discharging charged current generated in the formation of a sensor unit and, in the detection of photocurrent of a plurality of detection spots provided on a working electrode, discharging photocurrent which is derived from a detection spot subjected to the latest photocurrent measurement and becomes noise current. The present invention provides a measuring apparatus comprising a sensor unit comprising a working electrode, a counter electrode, and an electrolyte-containing substance, a single or plurality of light sources that apply light to the working electrode, an XY moving device provided when the light source is moved relatively in an XY direction relative to the working electrode, an ammeter that measures current which flows across the working electrode and the counter electrode, and a discharge device that discharges charged current and photocurrent derived from a detection spot subjected to the latest photocurrent measurement. The specific detection method using the measuring apparatus is carried out by controlling the timing of light irradiation and the timing of connection to the ammeter and the discharge device. | 04-26-2012 |
Patent application number | Description | Published |
20090294305 | Method for Specifically Detecting Analyte Using Photocurrent, and Electrode, Measuring Cell and Measuring Device for Use Therein - A method, an electrode, a measuring cell, and a measuring device are disclosed which can detect and quantitatively determine an analyte having specific bonding properties, in a highly sensitive, simple and accurate manner using photocurrent. This method comprises contacting a working electrode and a counter electrode with an electrolyte medium, wherein the working electrode has an analyte immobilized thereon through a probe substance and wherein the analyte is bonded to a sensitizing dye; irradiating the working electrode with light to photoexcite the sensitizing dye; and detecting photocurrent flowing between the working electrode and the counter electrode, wherein the photocurrent is generated by transfer of electrons from the photoexcited sensitizing dye to the working electrode. The working electrode comprises an electron accepting layer comprising an electron accepting substance capable of accepting electrons released from the sensitizing dye in response to photoexcitation, wherein the probe substance is supported on a surface of the electron accepting layer. The electron accepting substance is an oxide semiconductor having an energy level lower than that of a lowest unoccupied molecular orbit (LUMO) of the sensitizing dye. The electrolyte medium comprises an electrolyte and at least one solvent selected from an aprotic solvent and a protic solvent, wherein the electrolyte comprises a salt capable of providing an oxidized sensitizing dye with electrons. | 12-03-2009 |
20140238875 | METHOD FOR SPECIFICALLY DETECTING TEST SUBSTANCE - In detection of a test substance including a biomolecule by utilizing photocurrent detection of dye sensitization, disclosed is a detection method in which process of the photocurrent detection is carried out by using an electrolyte solution not necessarily requiring an organic solvent. By making the electrolyte medium an aqueous system not containing an organic solvent, not only its usability can be enhanced but also measurement values with less dispersion can be obtained. Therefore, detection of the test substance including a biomolecule by utilizing the photocurrent detection of dye sensitization according to the present invention is characterized by that the processes from a reaction process of a test substance till detection of the photocurrent are carried out in a single apparatus, and that process of the photocurrent detection is carried out by using an electrolyte solution not necessarily requiring an aprotic solvent. | 08-28-2014 |