Patent application number | Description | Published |
20110228000 | Printhead Assembly Employing Modular Printheads And Common Substrate Channel - A printhead assembly for a pagewidth printer includes a substrate channel; a plurality of printhead modules positioned in the channel to form a pagewidth printhead module assembly; an ink hose positioned within the substrate channel to supply the printhead modules with ink; an extrusion for housing bus bars providing electrical power to the printhead modules; a cover plate securing a flex printed circuit board (PCB) in the assembly, the PCB forming a data bus to the printhead modules; and compressible conductive strips provided between the busbars abutting contacts on an upper side of parts of the flex PCB. The substrate channel defines a series of groups of holes through which the printhead modules are supplied with differently colored inks. The hose defines parallel channels extending a length of the hose, the channels connected to ink containers at one end and sealed with a channel extrusion cap at the other end. | 09-22-2011 |
20110308313 | HUMIDITY SENSOR - A humidity sensor having a pair of electrodes spaced adjacent each other and defining an air gap therebetween such that the electrodes provide a capacitor with air as dielectric material, and, circuitry for sensing capacitance connected to the electrodes such that changes in permittivity of the air in the air gap caused by changes in humidity, cause changes in capacitance, wherein, the circuitry generates a signal indicative of humidity in the air in response to sensed capacitance of the electrodes. | 12-22-2011 |
20110308945 | GENETIC ANALYSIS LOC DEVICE WITH THICK ELECTRODES FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET SEQUENCES - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, a photosensor for sensing the photons emitted from the ECL luminophore, wherein, the photosensor has a planar active surface area for receiving the light from the ECL luminophore and the electrodes are between 0.25 micron and 2 microns thick in a direction normal to the planar active surface area of the photodiodes. | 12-22-2011 |
20110309275 | FAULT-TOLERANT MULTIPLE VALVE ASSEMBLY - A fault-tolerant multiple valve assembly for a microfluidic device, the multiple valve assembly having an inlet for receiving a liquid flowing through the microfluidic device, an outlet downstream of the inlet, a plurality of flow-paths extending from the inlet to the outlet, and, a plurality of valves positioned along each of the flow-paths respectively. | 12-22-2011 |
20110309276 | FAULT-TOLERANT MULTIPLE VALVE ASSEMBLY WITH THERMAL BOILING-INITIATED VALVE - A fault-tolerant multiple valve assembly for a microfluidic device, the multiple valve assembly having an inlet for receiving a liquid flowing through the microfluidic device, an outlet downstream of the inlet, a plurality of flow-paths extending from the inlet to the outlet, and, a plurality of valves positioned along each of the flow-paths respectively, wherein each valve has, a meniscus anchor for pinning a meniscus to arrest the flow of the liquid, and a valve heater for heating the meniscus such that the meniscus unpins from the meniscus anchor such that the liquid flow towards the outlet resumes. | 12-22-2011 |
20110311393 | MICROFLUIDIC DEVICE WITH THERMAL BEND ACTUATED PRESSURE PULSE VALVE - A microfluidic device having a channel having an inlet, an outlet and a meniscus anchor between the inlet and the outlet such that liquid flow from the inlet towards the outlet stops at the meniscus anchor where the liquid forms a meniscus, and, an actuator valve with a movable member for contacting the liquid, and a thermal expansion actuator for displacing the movable member to generate a pulse in the liquid to dislodge the meniscus such that the liquid flow towards the outlet resumes. | 12-22-2011 |
20110311394 | MICROFLUIDIC DEVICE WITH THERMAL BEND ACTUATED SURFACE TENSION VALVE - A microfluidic device having a channel having an inlet, an outlet and a meniscus anchor between the inlet and the outlet such that liquid flow from the inlet towards the outlet stops at the meniscus anchor where the liquid forms a meniscus, and, an actuator valve with a movable member for contacting the liquid, and an actuator for displacing the movable member from a quiescent position to an actuated position where the meniscus is extended into contact with a surface downstream of the meniscus anchor such that the liquid flow towards the outlet resumes. | 12-22-2011 |
20110311395 | MICROFLUIDIC DEVICE WITH ACTIVE VALVE AT REAGENT RESERVOIR OUTLET - A microfluidic device having a reservoir for containing a reagent, an outlet valve in fluid communication with the reservoir, the outlet valve having a meniscus anchor configured to form a meniscus that retains the reagent in the reservoir, and an actuator for receiving an activation signal and actuating in response to the activation signal such that the meniscus unpins from the meniscus anchor and the reagent flows out of the reservoir. | 12-22-2011 |
20110311407 | MICROFLUIDIC BOILING-INITIATED VALVE - A microfluidic valve having an inlet for receiving a flow of liquid, an outlet for outputting the flow of liquid, a meniscus anchor between the inlet and the outlet such that the flow from the inlet towards the outlet stops at the meniscus anchor where the liquid forms a meniscus, and, a valve heater for heating the meniscus such that the meniscus unpins from the meniscus anchor such that the flow towards the outlet resumes. | 12-22-2011 |
20110311408 | REAGENT DISPENSING APPARATUS - A reagent dispensing apparatus for loading reagents into a microfluidic device having a digital memory for data related to the reagents loaded into the microfluidic device, the reagent dispensing apparatus having a plurality of reagent vials each of the vials having an integrated circuit with memory storing data regarding the reagent in the vial, and a droplet dispenser, a mounting surface for detachably mounting the microfluidic device for movement relative to the vials, and, a control processor for operative control of the vials and the mounting surface, wherein, the control processor is configured to activate the droplet dispenser of the vial selected, move the vial into registration with the microfluidic device and download the data from the integrated circuit to the digital memory of the microfluidic device. | 12-22-2011 |
20110311409 | REAGENT DISPENSING APPARATUS WITH AUTOMATIC COLLECTION AND STORAGE OF REAGENT DATA - A reagent dispensing apparatus for loading reagents into a microfluidic device having a digital memory for data related to the reagents loaded into the microfluidic device, the reagent dispensing apparatus having a plurality of reagent vials each of the vials having an integrated circuit with memory storing data regarding the reagent in the vial, and a droplet dispenser, a mounting surface for detachably mounting the microfluidic device for movement relative to the vials, and, a control processor for operative control of the vials and the mounting surface, wherein, the control processor is configured to automatically interrogate each of the integrated circuits to collect and store the data regarding the reagents in each of the vials. | 12-22-2011 |
20110311411 | MICROFLUIDIC THERMAL BEND ACTUATED SURFACE TENSION VALVE - A microfluidic thermal bend actuated surface tension valve having an inlet for receiving a flow of liquid, an outlet for outputting the flow of liquid, a meniscus anchor between the inlet and the outlet such that the flow from the inlet towards the outlet stops at the meniscus anchor where the liquid forms a meniscus, a movable member for contacting the liquid, and, an actuator for displacing the movable member from a quiescent position to an actuated position where the meniscus is extended into contact with a surface downstream of the meniscus anchor such that the flow towards the outlet resumes. | 12-22-2011 |
20110311412 | MICROFLUIDIC THERMAL BEND ACTUATED PRESSURE PULSE VALVE - A microfluidic thermal bend actuated pressure pulse valve having an inlet for receiving a flow of liquid, an outlet for outputting the flow of liquid, a meniscus anchor between the inlet and the outlet such that the flow from the inlet towards the outlet stops at the meniscus anchor where the liquid forms a meniscus, a movable member for contacting the liquid, and, a thermal expansion actuator for displacing the movable member to generate a pulse in the liquid to dislodge the meniscus such that the liquid flow towards the outlet resumes. | 12-22-2011 |
20110311413 | FAULT-TOLERANT MULTIPLE VALVE ASSEMBLY WITH LIQUID DETECTOR SENSOR FEEDBACK - A fault-tolerant multiple valve assembly for a microfluidic device, the multiple valve assembly having an inlet for receiving a liquid flowing through the microfluidic device, an outlet downstream of the inlet, a plurality of flow-paths extending from the inlet to the outlet, a plurality of valves positioned along each of the flow-paths respectively, and, a sensor in each of the flow-paths respectively, the sensor being responsive to contact with the liquid. | 12-22-2011 |
20110311414 | FAULT-TOLERANT MULTIPLE VALVE ASSEMBLY WITH THERMAL BEND-ACTUATOR PRESSURE PULSE VALVE - A fault-tolerant multiple valve assembly for a microfluidic device, the multiple valve assembly having an inlet for receiving a liquid flowing through the microfluidic device, an outlet downstream of the inlet, a plurality of flow-paths extending from the inlet to the outlet, and, a plurality of valves positioned along each of the flow-paths respectively, wherein, each of the valves has a movable member for contacting the liquid, and a thermal expansion actuator for displacing the movable member to generate a pulse in the liquid to dislodge the meniscus such that the liquid flow towards the outlet resumes. | 12-22-2011 |
20110311415 | FAULT-TOLERANT MULTIPLE VALVE ASSEMBLY WITH THERMAL BEND-ACTUATOR SURFACE TENSION VALVE - A fault-tolerant multiple valve assembly for a microfluidic device, the multiple valve assembly having an inlet for receiving a liquid flowing through the microfluidic device, an outlet downstream of the inlet, a plurality of flow-paths extending from the inlet to the outlet, and, a plurality of valves positioned along each of the flow-paths respectively, wherein, each of the valves has a meniscus anchor and a meniscus relocation mechanism, the meniscus anchor being configured to form a meniscus that stops the liquid flow towards the outlet, and the meniscus relocation mechanism being configured to deform the meniscus at the meniscus anchor such that the meniscus contacts a surface downstream of the meniscus anchor and the liquid flow towards the outlet resumes. | 12-22-2011 |
20110311418 | MICROVIAL WITH DIGITAL MEMORY FOR STORAGE OF REAGENT SPECIFICATION DATA - A reagent microvial for a reagent dispensing apparatus, the microvial having a container for holding a volume of reagent, an integrated circuit with digital memory storing reagent data relating to specifications characterizing the reagent, and, electrical contacts for connection to a control processor in the reagent dispensing apparatus to provide the data to the control processor for download into devices supplied with the reagent. | 12-22-2011 |
20110312067 | DIALYSIS DEVICE FOR SEPARATING PATHOGENS FROM A BIOLOGICAL SAMPLE - A dialysis device to separate pathogens from a biological sample, the dialysis device having a first channel for receiving the biological sample, a second channel, and, a plurality of apertures, wherein, the second channel is fluidically connected to the first channel via the apertures such that the pathogens flow from the first channel to the second channel and larger constituents in the biological sample remain in the first channel. | 12-22-2011 |
20110312068 | DIALYSIS DEVICE FOR SEPARATING NUCLEATED CELLS IN A BIOLOGICAL SAMPLE FROM OTHER CONSTITUENTS - A dialysis device to separate nucleated cells in a biological sample from smaller constituents, the dialysis device having a first channel for receiving the biological sample, a second channel, and, a plurality of apertures, wherein, the second channel is fluidically connected to the first channel via the apertures, the apertures being smaller than the nucleated cells such that the smaller constituents flow from the first channel to the second channel and the nucleated cells in the biological sample remain in the first channel | 12-22-2011 |
20110312069 | MICROVIAL WITH DIGITAL MEMORY FOR STORAGE OF OLIGONUCLEOTIDE SPECIFICATION DATA - An oligonucleotide microvial for an oligonucleotide dispensing apparatus, the microvial having a container for holding a volume of reagent, an integrated circuit with digital memory storing oligonucleotide data relating to specifications characterizing the oligonucleotides, and, electrical contacts for connection to a control processor in the reagent dispensing apparatus to provide the data to the control processor for download into devices supplied with the oligonucleotides. | 12-22-2011 |
20110312070 | MICROFLUIDIC DEVICE WITH PCR CHAMBER FOR HIGH RATE OF TEMPERATURE CHANGE - A microfluidic device having a supporting substrate, a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having a PCR chamber, and, a heater element for heating the nucleic acid sequences within the PCR chamber, wherein during use, the heater element heats the nucleic acid sequences at a rate more than 80 K per second. | 12-22-2011 |
20110312071 | MICROFLUIDIC DEVICE WITH LARGE CHANNELS FOR CELL TRANSPORT AND SMALL CHANNELS SUITABLE FOR BIOCHEMICAL PROCESSES - A microfluidic device for molecular diagnostic analysis of a biological sample containing cells, the microfluidic device having a supporting substrate, a plurality of microsystems technology (MST) channels each with a cross-sectional area between 1 square micron and 400 square microns for biochemical processing of constituents within the biological sample, and, a plurality of cell transport channels, each with a cross-sectional area greater than 400 square microns for receiving the biological sample and transporting cells within the biological sample to predetermined sites in the MST channels. | 12-22-2011 |
20110312072 | MICROFLUIDIC DEVICE WITH SURFACE MICRO-MACHINED CHIPS AND INTERCONNECTING CAP - A microfluidic device for diagnostic analysis of a sample fluid, the microfluidic device having a plurality of lab-on-a-chip (LOC) devices each having a supporting substrate and a microsystems technology (MST) layer for processing the sample fluid, and, a cap interconnecting the plurality of LOC devices, the cap having a cap channel for establishing fluid communication between the MST layers on at least two of the LOC devices. | 12-22-2011 |
20110312073 | MICROFLUIDIC TEST MODULE INCORPORATING SURFACE MICRO-MACHINED CHIPS AND INTERCONNECTING CAP - A test module having a casing for hand held portability, a receptacle for receiving a sample fluid, and, a microfluidic device in fluid communication with the inlet, the microfluidic device having a plurality of lab-on-a-chip (LOC) devices and a cap in engagement with each of the plurality of LOC devices, wherein, the cap is configured to establish fluid communication between at least two of the LOC devices. | 12-22-2011 |
20110312074 | MICROFLUIDIC TEST MODULE WITH SAMPLE RECEPTACLE - A test module having a receptacle for receiving an unprocessed biological sample through an opening, a cover movable between open and closed positions, the cover exposing the opening when in the open position and closing the opening when in the closed position, and, a microfluidic device for processing the biological sample, the microfluidic device having a sample inlet, wherein, the receptacle is configured for fluid communication with the sample inlet such that the biological sample flows to the sample inlet by capillary action. | 12-22-2011 |
20110312075 | LOC DEVICE WITH PARALLEL INCUBATION AND PARALLEL DNA AND RNA AMPLIFICATION FUNCTIONALITY - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample containing genetic material including DNA and RNA, a supporting substrate, a plurality of reagent reservoirs, a first incubation section, the first incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, a second incubation section, the second incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material in parallel with the first incubation section, a first nucleic acid amplification section downstream of the first incubation section for amplifying at least some of the genetic material, and, a second nucleic acid amplification section downstream of the second incubation section for amplifying at least some of the genetic material in parallel with the first nucleic acid amplification section, wherein, the first incubation section, the second incubation section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312076 | MICROFLUIDIC TEST MODULE WITH FLEXIBLE MEMBRANE FOR INTERNAL MICROENVIRONMENT PRESSURE-RELIEF - A microfluidic test module having an outer casing for hand held portability, and, a microfluidic device mounted within the outer casing for processing a biological sample, the outer casing providing a microenvironment adjacent the microfluidic device, wherein, the outer casing has a membrane seal of flexible material between the microenvironment and atmosphere for reducing pressure changes in the microenvironment resulting from atmospheric pressure fluctuations. | 12-22-2011 |
20110312077 | LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET NUCLEIC ACID SEQUENCES IN A FLUID WITH CALIBRATION CHAMBER CONTAINING PROBES DESIGNED TO BE NON-COMPLEMENTARY WITH ANY NUCLEIC ACID SEQUENCES IN THE FLUID - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having hybridization chambers containing electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, at least one calibration chamber containing ECL probes designed to be non-complementary to any nucleic acid sequence in the fluid, and, electrodes for receiving an electrical pulse to excite the ECL luminophores. | 12-22-2011 |
20110312078 | MICROFLUIDIC DEVICE FOR DETECTING TARGET NUCLEIC ACID SEQUENCES IN MITOCHONDRIAL DNA - A microfluidic device for detecting mitochondrial DNA in a sample, the microfluidic device having an inlet for receiving a sample of biological material having cells with mitochondria containing mitochondrial DNA, a lysis section for lysing the mitochondria to release the mitochondrial DNA, probes for hybridization with target nucleic acid sequences in the mitochondrial DNA to form probe-target hybrids, and, a photosensor for detecting the probe-target hybrids. | 12-22-2011 |
20110312079 | LOC WITH DIGITAL MEMORY TO STORE EPIDEMIOLOGICAL UPDATES - A lab-on-a-chip (LOC) device for analyzing a sample fluid, the LOC device having a sample inlet for receiving the sample, a microsystems technology (MST) layer with functional sections for processing and analyzing the sample, and, CMOS circuitry with digital memory for storing epidemiological data, and configured to download epidemiological data updates from an external source. | 12-22-2011 |
20110312081 | REAGENT DISPENSING APPARATUS FOR ARRAY OF MICROFLUIDIC DEVICES - A reagent dispensing apparatus for loading reagents into a fixed array of microfluidic devices, each microfluidic device having a digital memory for data related to the reagents loaded into the microfluidic device, the reagent dispensing apparatus having a plurality of reagent vials each of the vials having an integrated circuit with memory storing data regarding the reagent in the vial, and a droplet dispenser, a mounting surface for detachably mounting the fixed array of microfluidic devices for movement relative to the vials, and, a control processor for operative control of the vials and the mounting surface, wherein, the control processor is configured to activate the droplet dispenser of the vial selected, move the vial into registration within one or more of the microfluidic devices within the fixed array and download the data from the integrated circuit to the digital memory of the one or more microfluidic devices. | 12-22-2011 |
20110312082 | DISPENSING APPARATUS FOR WAFER-SCALE DISPENSING OF REAGENTS - A reagent dispensing apparatus for loading reagents into a silicon wafer on which an array of lab-on-a-chip (LOC) devices are fabricated, each LOC device having a digital memory for data related to the reagents loaded into the LOC device, the reagent dispensing apparatus having a plurality of reagent vials each of the vials having an integrated circuit with memory storing data regarding the reagent in the vial, and a droplet dispenser, a mounting surface for detachably mounting the silicon wafer for movement relative to the vials, and, a control processor for operative control of the vials and the mounting surface, wherein, the control processor is configured to activate the droplet dispenser of the vial selected, move the vial into registration within one or more of the LOC devices on the silicon wafer and download the data from the integrated circuit to the digital memory of the one or more LOC devices. | 12-22-2011 |
20110312526 | METHOD OF ANALYSING THE NUCLEIC ACID CONTENT OF A BLOOD SAMPLE - A method of analyzing the nucleic acid content of a blood sample, the method comprising the steps of: providing a test module with an outer casing configured for handheld portability, the outer casing having a receptacle for receiving blood, the test module having a lysis section mounted in the outer casing for lysing cells and organisms in the blood to release the genetic material therein, a hybridization section with an array of probes for hybridization with target nucleic acid sequences in the genetic material, and circuitry for sensing which of the probes have hybridized and generating hybridization data, providing a test module reader for reading the hybridization data from the test module, inserting a blood sample in the receptacle, interfacing the test module with the test module reader, wherein, the test module reader analyses the nucleic acid content from the hybridization data. | 12-22-2011 |
20110312527 | METHOD OF ANALYSING THE NUCLEIC ACID CONTENT OF BIOLOGICAL FLUID - A method of analyzing the nucleic acid content of a biological fluid, the method comprising the steps of: providing a test module with an outer casing configured for handheld portability, the outer casing having a receptacle for receiving the biological fluid, the test module having a hybridization section with an array of probes for hybridization with target nucleic acid sequences in the biological fluid, and circuitry for sensing which of the probes have hybridized and generating hybridization data, providing a test module reader for reading the hybridization data from the test module, inserting the biological fluid in the receptacle, interfacing the test module with the test module reader, wherein, the test module reader analyses the nucleic acid content from the hybridization data. | 12-22-2011 |
20110312537 | LOC DEVICE FOR AMPLIFYING AND DETECTING TARGET NUCLEIC ACID SEQUENCES USING ELECTROCHEMILUMINESCENT RESONANT ENERGY TRANSFER, LINEAR PROBES WITH COVALENTLY ATTACHED PRIMERS - A lab-on-a-chip (LOC) device for amplifying and detecting target nucleic acid sequences, the LOC device having electrochemiluminescent (ECL), resonant energy transfer, linear probes for hybridization with the target nucleic acid sequences, each of the probes having a linear portion containing a sequence complementary to the target nucleic acid sequence, an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and a covalently attached primer for extension along a complementary sequence denatured from the target nucleic acid sequence to replicate the target nucleic acid sequence, heaters for thermally cycling the target nucleic acid sequences through a polymerase chain reaction (PCR), in which the covalently attached primers anneal to oligonucleotides containing the target nucleic acid sequences, and, electrodes for receiving an electrical pulse to excite the ECL luminophores, wherein during use, replicating the target nucleic acid sequence causes the linear portion to dissociate from the functional moiety such that the complementary nucleic acid sequence therein hybridizes to the target nucleic acid sequence and photons emitted by the ECL luminophore are not quenched. | 12-22-2011 |
20110312538 | LOC DEVICE WITH ELECTROCHEMILUMINESCENT PROBES FOR DETECTING TARGETS IN A FLUID AND A POSITIVE CONTROL PROBE FOR DETECTING A NUCLEIC ACID SEQUENCE KNOWN TO BE PRESENT - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, at least one positive control probe for detecting a nucleic acid sequence known to be always present in the fluid, and, electrodes for receiving an electrical pulse to excite the ECL luminophores. | 12-22-2011 |
20110312539 | LOC DEVICE WITH ELECTROCHEMILUMINESCENT PROBES FOR DETECTING TARGETS IN A FLUID AND A POSITIVE CONTROL PROBE WITHOUT A QUENCHER FOR LUMINOPHORE EMISSIONS - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, at least one positive control probe that has the ECL luminophore but not the functional moiety for quenching photon emission, and, electrodes for receiving an electrical pulse to excite the ECL luminophores. | 12-22-2011 |
20110312540 | LOC DEVICE FOR DETECTING TARGET NUCLEIC ACID SEQUENCES USING ELECTROCHEMILUMINESCENT PROBES AND CALIBRATION PROBES LACKING A LUMINOPHORE - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the ECL probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, calibration probes without an ECL luminophore, and, electrodes for receiving an electrical pulse to excite the ECL luminophores. | 12-22-2011 |
20110312541 | LOC FOR DETECTION OF HYBRIDIZATION OF NUCLEIC ACID SEQUENCES WITH PRIMER-LINKED LINEAR PROBES - A LOC device having a supporting substrate, a primer-linked, linear probe with a nucleic acid sequence that matches a target nucleic acid sequence, and a primer for elongating against the target nucleic acid sequence to form a complementary sequence such that during use the nucleic acid sequence matching the target nucleic acid sequence anneals to the complementary sequence to change a fluorescence emission from the probe in response to an excitation light, and, CMOS circuitry on the supporting substrate, the CMOS circuitry having operative control of the excitation light. | 12-22-2011 |
20110312542 | GENETIC ANALYSIS LOC WITH HYBRIDIZATION ARRAY WITH CALIBRATION CHAMBER CONTAINING CHAMBER WITH A BLOCKED INLET SPOTTED WITH REPORTER - A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing target nucleic acid sequences, hybridization chambers in fluid communication with the inlet, the hybridization chambers containing probes that each have a nucleic acid sequence for hybridization with the target nucleic acid sequences to form probe-target hybrids, a fluorophore and a quencher configured such that the fluorophore emits a fluorescence signal in response to an excitation light and the quencher quenches the fluorescence signal when the probe is not hybridized, but fails to quench the fluorescence signal from the probe-target hybrid, and, a calibration chamber not in fluid communication the inlet, wherein, the calibration chamber contains a fluorophore. | 12-22-2011 |
20110312544 | GENETIC ANALYSIS LOC WITH HYBRIDIZATION ARRAY WITH CALIBRATION CHAMBER CONTAINING PROBE THAT LACKS A REPORTER - A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing target nucleic acid sequences, hybridization chambers containing probes that each have a nucleic acid sequence for hybridization with the target nucleic acid sequences to form probe-target hybrids, a fluorophore and a quencher configured such that the fluorophore emits a fluorescence signal in response to an excitation light and the quencher quenches the fluorescence signal when the probe is not hybridized, but fails to quench the fluorescence signal from the probe-target hybrid, and, a calibration chamber with no fluorophore. | 12-22-2011 |
20110312545 | LOC DEVICE FOR PATHOGEN DETECTION WITH DIALYSIS, CHEMICAL LYSIS AND TANDEM NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the cells in the lysis section, a first nucleic acid amplification section downstream of the lysis section for amplifying first nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section downstream of the first nucleic acid amplification section for amplifying second nucleic acid sequences in the amplicon from the first nucleic acid amplification section, wherein, the dialysis section, the lysis section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312546 | LOC DEVICE FOR PATHOGEN DETECTION AND GENETIC ANALYSIS WITH CHEMICAL LYSIS, INCUBATION AND TANDEM NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for pathogen detection and genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a plurality of reagent reservoirs, a lysis section for lysing pathogens and leukocytes in the sample to release the genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the pathogens and leukocytes in the lysis section, an incubation section downstream of the lysis section, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, a first nucleic acid amplification section downstream of the incubation section for amplifying first nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section downstream of the first nucleic acid amplification section for amplifying second nucleic acid sequences in the amplicon from the first nucleic acid amplification section, wherein, the lysis section, the incubation section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312547 | MICROFLUIDIC DEVICE WITH REAGENT MIXING PROPORTIONS DETERMINED BY NUMBER OF ACTIVE OUTLET VALVES - A microfluidic device for testing a fluid, the microfluidic device having an inlet for receiving the fluid, a reservoir containing a reagent, a flow-path extending from the inlet, a plurality of outlet valves for fluid communication between the flow-path and the reservoir, each of the outlet valves having an actuator for opening the outlet valve in response to an activation signal, wherein during use, a number of the outlet valves are selectively opened such that the reagent flows into the flow-path to combine with the fluid from the inlet to produce a combined flow having a proportion of the reagent, the proportion of the reagent in the combined flow being determined by the number of the outlet valves opened. | 12-22-2011 |
20110312548 | TEST MODULE WITH DIFFUSIVE MIXING IN SMALL CROSS SECTIONAL AREA MICROCHANNEL - A test module for analysis of genetic material in a biological sample, the test module having an outer casing with an inlet for receiving the sample, a diffusion mixing section for diffusive mixing of the sample with at least one other liquid, the diffusion mixing section having a microchannel defining a flow-path for a combined flow of the sample and the at least one other liquid, wherein, the channel cross section transverse to the flow direction is less than 100,000 square microns. | 12-22-2011 |
20110312549 | MICROFLUIDIC DEVICE WITH MULTI-LAYER DIALYSIS SECTION - A microfluidic device for dialysis of a fluid sample, the microfluidic device having a first layer of material defining a first channel and a second channel, the first channel configured for receiving the sample which contains constituents of different sizes, a second layer having a plurality of apertures open to the first channel and at least one fluid connection leading from the apertures to the second channel for establishing fluid communication between the first channel and the second channel, wherein, the apertures are sized in accordance with a predetermined size threshold such that the constituents flowing to the second channel are smaller constituents that are smaller than the predetermined size threshold, and the constituents retained in the first channel include larger constituents which are larger than the predetermined size threshold. | 12-22-2011 |
20110312550 | LOC DEVICE FOR GENETIC ANALYSIS WHICH PERFORMS NUCLEIC ACID AMPLIFICATION AFTER SAMPLE PREPARATION IN A DIALYSIS SECTION - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating small constituents from larger constituents in the sample, a plurality of reagent reservoirs, a nucleic acid amplification section downstream of the dialysis section for amplifying nucleic acid sequences in the sample, wherein, the dialysis section and the nucleic acid amplification section are both supported on the supporting substrate. | 12-22-2011 |
20110312551 | LOC DEVICE FOR GENETIC ANALYSIS WHICH PERFORMS NUCLEIC ACID AMPLIFICATION BEFORE REMOVING NON-NUCLEIC ACID CONSTITUENTS IN A DIALYSIS SECTION - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a plurality of reagent reservoirs, a nucleic acid amplification section downstream of the incubation section for amplifying nucleic acid sequences in the sample, and, a dialysis section downstream of the nucleic acid amplification section for prehybridization filtration of amplicon produced by the nucleic acid amplification section, the dialysis section being configured to remove cell debris from the amplicon, wherein, the nucleic acid amplification section and the dialysis section are both supported on the supporting substrate. | 12-22-2011 |
20110312552 | MICROFLUIDIC DEVICE WITH CONDUCTIVITY SENSOR - A microfluidic device for processing a fluid, the microfluidic device having an inlet for receiving the fluid, functional sections for processing the fluid, a flow-path extending from the inlet into at least some of the functional sections, CMOS circuitry for operative control of the functional sections, and, a conductivity sensor with a first terminal and a second terminal spaced apart along the flow-path, and a first electrode and a second electrode positioned between the first terminal and the second terminal and spaced apart along the flow-path, wherein, the CMOS circuitry is configured to generate a current between the first terminal and the second terminal, and measure a voltage across the first electrode and the second electrode such that conductivity of the fluid in the flow-path is derived from the current and the measured voltage. | 12-22-2011 |
20110312553 | MICROFLUIDIC DEVICE WITH NON-IMAGING OPTICS FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGETS - A microfluidic device for detecting target molecules in a fluid, the microfluidic device having an array of chambers containing electrochemiluminescent (ECL) probes for reaction with the target molecules to form a probe-target complexes, electrodes positioned in each of the chambers for receiving an electrical pulse, the probe-target complexes being configured to emit a photon of light when excited by current between the electrodes, and, a photosensor for detecting the light emitted by the probes, wherein, the photosensor is less than 1600 microns from the probes. | 12-22-2011 |
20110312554 | MICROFLUIDIC DEVICE WITH DIALYSIS DEVICE, LOC AND INTERCONNECTING CAP - A microfluidic device for processing a sample fluid containing target molecules, the microfluidic device having a dialysis device for receiving the sample and concentrating the target molecules in a portion of the sample, a lab-on-a-chip (LOC) for analyzing the target molecules, and, a cap overlaying the LOC and the dialysis device for establishing fluid communication between the LOC and the dialysis device. | 12-22-2011 |
20110312555 | LOC DEVICE FOR DETECTING HYBRIDIZATION OF TARGET NUCLEIC ACID SEQUENCES WITH ELECTROCHEMILUMINESCENT RESONANT ENERGY TRANSFER, PRIMER-LINKED, STEM-AND-LOOP PROBES - A lab-on-a-chip (LOC) device for detecting hybridization of target nucleic acid sequences, the LOC device having electrochemiluminescent (ECL), resonant energy transfer, primer-linked, stem-and-loop probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, each of the probes having a loop portion containing the target nucleic acid sequence, a primer for extension along the target nucleic acid sequence to form a nucleic acid sequence complementary to the target, an ECL luminophore for emitting photons when in an excited state, and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and, electrodes for receiving an electrical pulse to excite the ECL luminophores, wherein during use, forming the complementary nucleic acid sequence causes the loop portion to open such the target nucleic acid sequence therein hybridizes to the complementary nucleic acid sequence and the ECL luminophore is moved away from the functional moiety. | 12-22-2011 |
20110312556 | MICROFLUIDIC DEVICE WITH TRIGGER PHOTODIODE IN EACH HYBRIDIZATION CHAMBER - A microfluidic device having a supporting substrate, a hybridization chamber containing a probe having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, CMOS circuitry between the supporting substrate and the hybridization chamber, the CMOS circuitry having a photodiode for generating an output signal in response to the fluorescence signal, and a trigger photodiode for generating an output in response to the excitation light, wherein, the CMOS circuitry is configured to activate the photodiode when the trigger photodiode indicates the excitation light has deactivated. | 12-22-2011 |
20110312557 | LOC DEVICE FOR PATHOGEN DETECTION WITH DIALYSIS, LYSIS AND PARALLEL NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, a first nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material in a first portion of the sample flow from the lysis section, and, a second nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material in a second portion of the sample flow from the lysis section, wherein, the dialysis section, the lysis section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312558 | LOC DEVICE FOR PATHOGEN DETECTION WITH DIALYSIS, LYSIS AND TANDEM NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, a first nucleic acid amplification section downstream of the lysis section for amplifying first nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section downstream of the first nucleic acid amplification section for amplifying second nucleic acid sequences in the amplicon from the first nucleic acid amplification section, wherein, the dialysis section, the lysis section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312559 | LOC DEVICE FOR PATHOGEN DETECTION WITH DIALYSIS, THERMAL LYSIS AND PARALLEL NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, the lysis section having a lysis chamber and a heater for lysing the pathogens while the sample is in the lysis chamber, a first nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material in a first portion of the sample flow from the lysis section, and, a second nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material in a second portion of the sample flow from the lysis section, wherein, the dialysis section, the lysis section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312560 | LOC DEVICE FOR PATHOGEN DETECTION WITH DIALYSIS, THERMAL LYSIS AND TANDEM NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, the lysis section having a lysis chamber and a heater for lysing the pathogens while the sample is in the lysis chamber, a first nucleic acid amplification section downstream of the lysis section for amplifying first nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section downstream of the first nucleic acid amplification section for amplifying second nucleic acid sequences in the amplicon from the first nucleic acid amplification section, wherein, the dialysis section, the lysis section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312561 | MICROFLUIDIC DEVICE WITH PHOTODIODES WITH CONTROLLABLE SHUNTS TO DETECT FLUORESCING HYBRIDIZED PROBES - A microfluidic device for detecting a target nucleic acid sequence, the microfluidic device having a probe for hybridization with the target nucleic acid sequence to form a probe-target hybrid, the probe having a fluorophore for generating fluorescence emissions in response to an excitation light, a photodiode for detecting the fluorescence emissions, a shunt transistor between the photodiode and a voltage source, and, CMOS circuitry for controlling the shunt transistor to remove carriers generated by absorption of photons of the excitation light in the photodiode. | 12-22-2011 |
20110312562 | LOC DEVICE FOR PATHOGEN DETECTION WITH DIALYSIS, CHEMICAL LYSIS AND PARALLEL NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the cells in the lysis section, a first nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material in a first portion of the sample flow from the lysis section, and, a second nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material in a second portion of the sample flow from the lysis section, wherein, the dialysis section, the lysis section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312563 | LOC DEVICE FOR DETECTING TARGET NUCLEIC ACID SEQUENCES IN A FLUID USING HYBRIDIZATION CHAMBER ARRAY AND NEGATIVE CONTROL CHAMBER CONTAINING ELECTROCHEMILUMINESCENT PROBE DESIGNED TO BE NON-COMPLEMENTARY TO ANY SEQUENCE IN THE FLUID - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, hybridization chambers containing the probes for detection of the targets, and a pair of the electrodes, and, at least one negative control chamber containing negative control probes that are incapable of hybridization with any nucleic acid sequences in the fluid. | 12-22-2011 |
20110312564 | LOC DEVICE FOR GENETIC ANALYSIS WITH DIALYSIS, CHEMICAL LYSIS, INCUBATION AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating cells larger than a predetermined threshold in the sample from smaller constituents, whereby the cells larger than a predetermined threshold include target cells containing genetic material for analysis, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the target cells to release the genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the target cells in the lysis section, an incubation section downstream of the lysis section, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, and, a nucleic acid amplification section downstream of the incubation section for amplifying nucleic acid sequences from the genetic material, wherein, the dialysis section, the lysis section, the incubation section and the nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312565 | LOC DEVICE FOR DETECTING TARGET NUCLEIC ACID SEQUENCES USING HYBRIDIZATION CHAMBER ARRAY AND NEGATIVE CONTROL CHAMBER CONTAINING PROBES WITHOUT ELECTROCHEMILUMINESCENT REPORTER - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, hybridization chambers containing the probes for detection of the targets, and a pair of the electrodes, and, at least one negative control chamber containing negative control probes without an ECL luminophore. | 12-22-2011 |
20110312566 | LOC DEVICE FOR GENETIC ANALYSIS WITH DIALYSIS, CHEMICAL LYSIS, INCUBATION AND PARALLEL NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating cells larger than a predetermined threshold in the sample from smaller constituents, whereby the cells larger than a predetermined threshold include target cells containing genetic material for analysis, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the target cells to release the genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the target cells in the lysis section, an incubation section downstream of the lysis section, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, a first nucleic acid amplification section downstream of the incubation section for amplifying nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section downstream of the incubation section for amplifying nucleic acid sequences in the genetic material in parallel with the first nucleic acid amplification section, wherein, the dialysis section, the lysis section, the incubation section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312567 | LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET NUCLEIC ACID SEQUENCES USING HYBRIDIZATION CHAMBER ARRAY AND NEGATIVE CONTROL CHAMBER WITHOUT PROBES - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, hybridization chambers containing the probes for detection of the targets, and a pair of the electrodes, and, at least one negative control chamber without the ECL probes. | 12-22-2011 |
20110312568 | LOC DEVICE FOR GENETIC ANALYSIS WITH DIALYSIS, CHEMICAL LYSIS, INCUBATION AND TANDEM NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating cells larger than a predetermined threshold in the sample from smaller constituents, whereby the cells larger than a predetermined threshold include target cells containing genetic material for analysis, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the target cells to release the genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the target cells in the lysis section, an incubation section downstream of the lysis section, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, a first nucleic acid amplification section downstream of the incubation section for amplifying first nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section downstream of the first nucleic acid amplification section for amplifying second nucleic acid sequences in the amplicon from the first nucleic acid amplification section, wherein, the dialysis section, the lysis section, the incubation section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312569 | MICROFLUIDIC DEVICE WITH SMALL CROSS SECTIONAL AREA MICROCHANNEL - A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section with a PCR microchannel for thermally cycling the sample to amplify the nucleic acid sequences, the PCR microchannel defining a flow-path for the sample such that flow of the sample along the PCR microchannel is driven by capillary action, and, the PCR microchannel has a cross sectional area transverse to the flow less than 100,000 square microns. | 12-22-2011 |
20110312570 | MICROFLUIDIC DEVICE FOR DETECTING TARGET NUCLEIC ACID SEQUENCES WITH PROBES HAVING LONG FLUORESCENCE LIFETIME FLUOROPHORES - A microfluidic device for detecting a target nucleic acid sequence, the microfluidic device having a probe for hybridization with the target nucleic acid sequence to form a probe-target hybrid, the probe having a fluorophore for generating fluorescence emissions in response to an excitation light, a photodiode for detecting the fluorescence emissions, and, CMOS circuitry activating the photodiode, wherein, the fluorophore has a fluorescence lifetime longer than 100 nanoseconds. | 12-22-2011 |
20110312572 | LOC DEVICE FOR PATHOGEN DETECTION AND GENETIC ANALYSIS WITH CHEMICAL LYSIS, INCUBATION AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for pathogen detection and genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a plurality of reagent reservoirs, a lysis section for lysing pathogens and leukocytes in the sample to release the genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the pathogens and leukocytes in the lysis section, an incubation section downstream of the lysis section, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, and, a nucleic acid amplification section downstream of the incubation section for amplifying nucleic acid sequences from the genetic material, wherein, the lysis section, the incubation section and the nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312573 | LOC DEVICE FOR PATHOGEN DETECTION AND GENETIC ANALYSIS WITH CHEMICAL LYSIS, INCUBATION AND PARALLEL NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for pathogen detection and genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a plurality of reagent reservoirs, a lysis section for lysing pathogens and leukocytes in the sample to release the genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the pathogens and leukocytes in the lysis section, an incubation section downstream of the lysis section, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, a first nucleic acid amplification section downstream of the incubation section for amplifying nucleic acid sequences in the genetic material in a first portion of the sample flow from the incubation section, and, a second nucleic acid amplification section downstream of the incubation section for amplifying nucleic acid sequences in the genetic material in a second portion of the sample flow from the incubation section, wherein, the lysis section, the incubation section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312574 | LOC DEVICE FOR PATHOGEN DETECTION, GENETIC ANALYSIS AND PROTEOMIC ANALYSIS WITH DIALYSIS, CHEMICAL LYSIS, INCUBATION AND PARALLEL NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for pathogen detection, genetic analysis and proteomic analysis of a biological sample, using a leukocyte dialysis section for separating out a leukocyte stream, a pathogen dialysis section for separating out a pathogen stream. A leukocyte lysis section lyses the leukocytes and a leukocyte incubation section allows enzymatic reaction of the genetic material with enzymes. A pathogen lysis section lyses the pathogens and, a pathogen incubation section allows enzymatic reaction of the genetic material with enzymes. An erythrocyte lysis section lyses the erythrocytes. First and second leukocyte nucleic acid amplification sections amplify nucleic acid sequences in parallel. First and second pathogen nucleic acid amplification sections amplify nucleic acid sequences in parallel. | 12-22-2011 |
20110312575 | GENETIC ANALYSIS LOC FOR NUCLEIC ACID AMPLIFICATION USING A NICKING ENZYME AND A DNA POLYMERASE - A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing dNTPs, primers, nicking enzymes, buffer solution and DNA polymerase for addition to the sample to form an amplification mixture, and, a nucleic acid amplification section for maintaining the amplification mixture at a predetermined temperature during isothermal amplification of the target nucleic acid sequences. | 12-22-2011 |
20110312576 | GENETIC ANALYSIS LOC DEVICE FOR MULTI-STAGE AMPLIFICATION OF NUCLEIC ACID SEQUENCES - A lab-on-a-chip (LOC) device for genetic analysis of nucleic acid sequences in a sample, the LOC device having a sample inlet for receiving the sample, a first polymerase chain reaction (PCR) section for thermal cycling a first PCR mixture of dNTP's, primers, and buffer solution together with the sample and polymerase, to amplify the nucleic acid sequences, and, a second PCR section downstream of the first PCR section for thermally cycling a second PCR mixture of dNTPs, primers, and buffer solution together with polymerase, and at least some of the amplicon from the first PCR section. | 12-22-2011 |
20110312577 | TEST MODULE WITH LOW-VOLUME HYBRIDIZATION CHAMBERS AND REAGENT RESERVOIR FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET NUCLEIC ACID SEQUENCES - A test module for detecting target nucleic acid sequences in a fluid, the test module having an outer casing having an inlet for receiving the fluid containing the target nucleic acid sequences, a hybridization chamber mounted in the casing, the hybridization chamber containing electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the ECL probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, and, a reagent reservoir containing a reagent for addition to the fluid prior to detection of the target nucleic acid sequences, wherein, the hybridization chamber has a volume less than 900,000 cubic microns, and, the reagent reservoir has a volume less than 1000,000,000 cubic microns. | 12-22-2011 |
20110312578 | GENETIC ANALYSIS LOC FOR NON-SPECIFIC NUCLEIC ACID AMPLIFICATION PRIOR TO SPECIFIC AMPLIFICATION OF PARTICULAR SEQUENCES - A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing dNTP's, primers, polymerase and buffer solution for addition to the sample, and, a nucleic acid amplification section for thermal control of the sample to perform a first stage amplification of the target nucleic acid sequences and subsequent thermal control of amplicon from the first stage amplification, to perform a second stage amplification for further amplifying the target nucleic acid sequences. | 12-22-2011 |
20110312579 | LOC DEVICE WITH PARALLEL INCUBATION AND PARALLEL NUCLEIC ACID AMPLIFICATION FUNCTIONALITY - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample containing genetic material, a supporting substrate, a plurality of reagent reservoirs, a first incubation section, the first incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, a second incubation section, the second incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material in parallel with the first incubation section, a first nucleic acid amplification section downstream of the first incubation section for amplifying nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section downstream of the second incubation section for amplifying nucleic acid sequences in the genetic material in parallel with the first nucleic acid amplification section, wherein, the first incubation section, the second incubation section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312580 | LOC DEVICE WITH NUCLEIC ACID AMPLIFICATION SECTION AND THERMAL INSULATION TRENCH - A lab-on-a-chip (LOC) device for amplifying nucleic acid sequences in a sample, the LOC device having a supporting substrate, a plurality of functional sections for processing the sample, one of the functional sections being a nucleic acid amplification section for amplifying nucleic acid sequences in the sample, wherein, the nucleic acid amplification section is supported on the supporting substrate and the supporting substrate defines a trench for thermally insulating the nucleic acid amplification section from one or more of the other functional sections. | 12-22-2011 |
20110312581 | MICROFLUIDIC DEVICE WITH NUCLEIC ACID AMPLIFICATION CHAMBER HEATER BONDED TO CHAMBER INTERIOR - A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, and, a nucleic acid amplification section for amplifying the nucleic acid sequences, the nucleic acid amplification section having an amplification chamber and a heater element, wherein, the heater element is bonded to an interior surface of the amplification chamber. | 12-22-2011 |
20110312582 | TEST MODULE WITH NUCLEIC ACID AMPLIFICATION SECTION - A test module for amplifying nucleic acid sequences, the test module having an outer casing with receptacle for receiving a sample containing genetic material, a plurality of reagent reservoirs containing reagents for addition to the sample, and, a nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material. | 12-22-2011 |
20110312583 | TEST MODULE WITH PARALLEL NUCLEIC ACID AMPLIFICATION SECTIONS - A test module for amplifying nucleic acid sequences, the test module having an outer casing with receptacle for receiving a sample containing genetic material, a plurality of reagent reservoirs containing reagents for addition to the sample, a first nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material in parallel with the first nucleic acid amplification section. | 12-22-2011 |
20110312584 | SINGLE-USE TEST MODULE WITH DRIVER FOR EXCITATION OF ELECTROCHEMILUMINESCENT LUMINOPHORES - A test module for detecting target nucleic acid sequences in a fluid, the test module having an outer casing with a receptacle for receiving a fluid containing the target nucleic acid sequences, an array of electrochemiluminescent (ECL) probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, and, electrodes positioned for receiving an electrical pulse, the probe-target hybrids being configured to emit photons when excited by current between the electrodes, and, control circuitry for providing the electrodes with the electrical pulse, wherein during use, addition of the fluid to the probes prevents subsequent addition of other fluid to the probes. | 12-22-2011 |
20110312585 | MICROFLUIDIC DEVICE WITH PARALLEL DNA AND RNA AMPLIFICATION SECTION - A microfluidic device for amplifying DNA and RNA, the microfluidic device having an inlet for receiving a sample containing genetic material including DNA and RNA, a plurality of reagent reservoirs containing reagents for addition to the sample, a first nucleic acid amplification section for amplifying at least some of the genetic material, and, a second nucleic acid amplification section for amplifying at least some of the genetic material in parallel with the first nucleic acid amplification section. | 12-22-2011 |
20110312586 | MICROFLUIDIC DEVICE FOR CHEMICALLY AND THERMALLY LYSING CELLS - A microfluidic device for lysing cells in a fluid, the microfluidic device having a supporting substrate, an inlet for receiving fluid containing cells, a lysis section in fluid communication with the inlet, the lysis section having at least one heater for heating the fluid, a reservoir containing a lysis reagent, and, CMOS circuitry between the supporting substrate and the lysis section, the CMOS circuitry being configured for selectively lysing the cells using thermal lysis in the lysis section, chemically lysing the cells using the lysis reagent, or both chemically and thermally lysing the cells using the lysis section and the lysis reagent. | 12-22-2011 |
20110312587 | LOC FOR DETECTION OF HYBRIDIZATION OF NUCLEIC ACID SEQUENCES WITH PRIMER-LINKED STEM-AND-LOOP PROBES - A LOC device having a supporting substrate, a primer-linked, stem-and-loop probe incorporating a nucleic acid sequence that matches a target nucleic acid sequence, and a primer for elongating against the target nucleic acid sequence to form a complementary sequence such that during use the probe nucleic acid sequence matching the target nucleic acid sequence anneals to the complementary sequence to change a fluorescence emission from the probe in response to an excitation light, and, CMOS circuitry on the supporting substrate, the CMOS circuitry having operative control of the excitation light. | 12-22-2011 |
20110312588 | LOC DEVICE WITH ON-CHIP SEMICONDUCTOR CONTROLLED INCUBATION SECTION - A lab-on-a-chip (LOC) device having a supporting substrate, a sample inlet for receiving a fluid sample, an incubation section in fluid communication with the sample inlet, the incubation section having at least one heater, and, CMOS circuitry between the supporting substrate and the incubation section, wherein, the CMOS circuitry is connected to the at least one heater for maintaining the fluid sample at an incubation temperature for an incubation period. | 12-22-2011 |
20110312589 | GENETIC TEST MODULE WITH LOW OLIGONUCLEOTIDE PROBE MASS AND REAGENT VOLUMES - A test module for performing a genetic diagnostic assay, the test module having an outer casing dimensioned for hand-held portability, the outer casing having a receptacle for a biological sample containing target nucleic acid sequences, an array of chambers containing probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, a flow-path extending from the inlet to the probes, and, a reagent reservoir containing a reagent for addition to the sample in the flow-path upstream of the probes, wherein, each of the chambers contains less than 270 picograms of probe and the reagent reservoir has a volume less than 1000,000,000 cubic microns. | 12-22-2011 |
20110312590 | MICROFLUIDIC DEVICE WITH ELONGATE INCUBATION CHAMBER - A microfluidic device having a sample inlet for receiving a fluid sample, an incubation section having an elongate incubation chamber with a longitudinal extent much greater than the lateral dimensions, and at least one heater for maintaining the fluid sample at an incubation temperature for an incubation period, wherein, the at least one heater is also elongated with a lateral extent parallel to that of the elongate incubation chamber. | 12-22-2011 |
20110312591 | LOC WITH LOW-VOLUME HYBRIDIZATION CHAMBER AND REAGENT RESERVOIR FOR GENETIC ANALYSIS - A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing a target nucleic acid sequence, a reagent reservoir containing a reagent for addition to the biological sample, and, a hybridization chamber containing probes having a nucleic acid sequence for hybridization with the target nucleic acid sequence to form probe-target hybrids, wherein, microsystems tech the reagent reservoir has a volume less than 1,000,000,000 cubic microns and the hybridization chamber has a volume less than 900,000 cubic microns. | 12-22-2011 |
20110312592 | MICROFLUIDIC DEVICE WITH INCUBATION CHAMBER BETWEEN SUPPORTING SUBSTRATE AND HEATER - A microfluidic device having a supporting substrate, a sample inlet for receiving a fluid sample, an incubation section having an incubation chamber, and at least one heater for maintaining the fluid sample at an incubation temperature for a period, wherein, the incubation chamber is between the at least one heater element and the supporting substrate. | 12-22-2011 |
20110312593 | MICROFLUIDIC DEVICE WITH INCUBATOR HAVING TWO-DIMENSIONAL CONTROL OF INPUT HEAT FLUX - A microfluidic device having an inlet for receiving a fluid, an incubation section having a microchannel configured to have a plurality of mutually parallel, adjacent channel sections, and a plurality of elongate heaters positioned end to end along each of the channel sections, wherein, each of the heaters are independently operable for two-dimensional control of heat flux density to the incubation section. | 12-22-2011 |
20110312594 | GENETIC ANALYSIS LOC WITH HYBRIDIZATION PROBES INCLUDING POSITIVE AND NEGATIVE CONTROL PROBES - A lab-on-a-chip (LOC) device for genetic analysis of nucleic acid sequences extracted from biological material, the LOC device having probes for hybridization with target nucleic acid sequences within the nucleic acid sequences to form probe-target hybrids, the probe-target hybrids each having a reporting fluorophore for emitting a fluorescence signal in response to an excitation light, a positive control probe configured to always emit a fluorescence signal, and, a negative control probe configured to never emit a fluorescence signal, wherein during use, detecting a fluorescence signal from the negative control probe indicates a malfunction, and, not detecting a fluorescence signal from the positive control probe indicates a malfunction. | 12-22-2011 |
20110312596 | MICROFLUIDIC DEVICE WITH SURFACE TENSION VALVE AT REAGENT RESERVOIR OUTLET - A microfluidic device for processing a fluid sample, the microfluidic device having a reservoir for containing a reagent, and, a surface tension valve having an aperture configured to pin a meniscus of the reagent such that the meniscus retains the reagent in the reagent reservoir until contact with the fluid sample removes the meniscus such that the reagent flows out of the reagent reservoir. | 12-22-2011 |
20110312597 | GENETIC ANALYSIS LOC WITH HYBRIDIZATION ARRAY WITH POSITIVE CONTROL CHAMBERS INCORPORATING PROBES WITH NO QUENCHERS - A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing a target nucleic acid sequence, probes that each have a nucleic acid sequence for hybridization with the target nucleic acid sequence to form a probe-target hybrid, a fluorophore and a quencher configured such that the fluorophore emits a fluorescence signal in response to an excitation light and the quencher quenches the fluorescence signal when the probe is not hybridized, but fails to quench the fluorescence signal from the probe-target hybrid, and, a control probe with a fluorophore but no quencher, wherein, the control probe always emits the fluorescence signal in response to the excitation light. | 12-22-2011 |
20110312598 | MICROFLUIDIC DEVICE WITH REAGENT MIXING PROPORTIONS DETERMINED BY OUTLET VALVE NUMBERS - A microfluidic device for testing a fluid, the microfluidic device having an inlet for receiving the fluid, a reservoir containing a reagent, a flow-path extending from the inlet, a valve assembly for establishing a fluid connection between the flow-path and the reservoir, the valve assembly having a plurality of outlet valves and a plurality of channels from the reservoir to the flow-path, wherein during use, a number of the outlet valves open such that the reagent flows through the valve assembly to the flow-path to combine with the fluid from the inlet to produce a combined flow having a proportion of the reagent, the proportion of the reagent in the combined flow being determined by the number of the outlet valves opened. | 12-22-2011 |
20110312599 | MICROFLUIDIC DEVICE WITH A PCR SECTION WITH SINGLE ACTIVATION, OUTLET VALVE - A microfluidic device for amplifying nucleic acid sequences, the microfluidic device having a polymerase chain reaction (PCR) section for thermally cycling the nucleic acid sequences and a PCR mix of reagents through a denaturation temperature, an annealing temperature and a primer extension temperature, and, a PCR outlet valve to retain the nucleic acid sequences and a PCR mix of reagents in the PCR section during the thermal cycling, wherein, the PCR outlet valve is configured to open in response to an activation signal such that amplicon can flow from the PCR section and once open, the PCR outlet valve is unable to close. | 12-22-2011 |
20110312600 | GENETIC ANALYSIS LOC WITH THERMAL BEND ACTUATED PRESSURE PULSE VALVE - A lab-on-a-chip device (LOC) for genetic analysis having a supporting substrate, an array of probes for hybridization with target nucleic acid sequences to form probe-target hybrids, a channel for directing a flow of liquid containing the target nucleic acid sequences, the channel having an inlet, an outlet and a meniscus anchor between the inlet and the outlet such that the flow from the inlet towards the outlet stops at the meniscus anchor where the liquid forms a meniscus, and, an actuator valve with a movable member for contacting the liquid, and a thermal expansion actuator for displacing the movable member to generate a pulse in the liquid to dislodge the meniscus such that the liquid flow towards the outlet resumes. | 12-22-2011 |
20110312601 | LOC DEVICE WITH DIGITAL MEMORY FOR SECURE STORAGE OF DATA - A lab-on-a-chip (LOC) device for analyzing a sample fluid, the LOC device having a sample inlet for receiving the sample, a microsystems technology (MST) layer with functional sections for processing and analyzing the sample, and, CMOS circuitry with digital memory for storing data and operational information to operatively control the functional sections during processing and analysis of the sample, wherein, the data is encrypted for secure communication with an external device. | 12-22-2011 |
20110312602 | GENETIC ANALYSIS LOC WITH THERMAL BEND ACTUATED SURFACE TENSION VALVE - A lab-on-a-chip (LOC) device for genetic analysis having a supporting substrate, an array of probes for hybridization with target nucleic acid sequences to form probe-target hybrids, a channel for directing a flow of liquid containing the target nucleic acid sequences, the channel having an inlet, an outlet and a meniscus anchor between the inlet and the outlet such that the flow from the inlet towards the outlet stops at the meniscus anchor where the liquid forms a meniscus, and, an actuator valve with a movable member for contacting the liquid, and an actuator for displacing the movable member from a quiescent position to an actuated position where the meniscus is extended into contact with a surface downstream of the meniscus anchor such that the liquid flow towards the outlet resumes. | 12-22-2011 |
20110312603 | TEST MODULE WITH LOC HAVING ON-CHIP ELECTRONICS FOR MODULE CONTROL - A test module for analyzing a sample fluid, the test module having a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a supporting substrate, and, CMOS circuitry on the supporting substrate for operative control of the functional sections during processing and analysis of the sample. | 12-22-2011 |
20110312604 | LOC HAVING ON-CHIP ELECTRONICS FOR USE IN A TEST MODULE TO CONTROL MODULE COMMUNICATIONS - A lab-on-a-chip (LOC) device for installation in a test module for analyzing a sample fluid and communicating test results to an external device, the LOC device having a supporting substrate, a sample inlet for receiving the sample, a microsystems technology (MST) layer with functional sections for processing and analyzing the sample, and, CMOS circuitry on the supporting substrate for operative control of a communication interface in the test module for communication with the external device. | 12-22-2011 |
20110312605 | LOC DEVICE WITH INTEGRAL CONTROLLER - A lab-on-a-chip (LOC) device for analyzing a sample fluid, the LOC device having a supporting substrate, a sample inlet for receiving the sample, a microsystems technology (MST) layer with functional sections for processing and analyzing the sample, and, CMOS circuitry between the supporting substrate and the MST layer, the CMOS circuitry having a controller to control operations performed by the functional sections during processing and analysis of the sample. | 12-22-2011 |
20110312606 | LOC DEVICE WITH DIGITAL MEMORY - A lab-on-a-chip (LOC) device for analyzing a sample fluid, the LOC device having a supporting substrate, a sample inlet for receiving the sample, a microsystems technology (MST) layer with functional sections for processing and analyzing the sample, and, CMOS circuitry between the supporting substrate and the MST layer, the CMOS circuitry having digital memory for storing data and operational information to operatively control the functional sections during processing and analysis of the sample. | 12-22-2011 |
20110312607 | GENETIC ANALYSIS LOC WITH HYBRIDIZATION ARRAY WITH CALIBRATION PHOTOSENSOR OUTPUT SUBTRACTED IN A DIFFERENTIAL CIRCUIT FROM THE OUTPUT OF HYBRIDIZATION PHOTOSENSORS - A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing target nucleic acid sequences, probes that each have a nucleic acid sequence for hybridization with a respective one of the target nucleic acid sequences to form a probe-target hybrid and emit a fluorescence signal in response to an excitation light, and, photosensors corresponding to each of the probes respectively, and, a calibration photosensor not corresponding to any of the probes such that output from the calibration photosensor is subtracted in a differential circuit from each output from the photosensors. | 12-22-2011 |
20110312608 | TEST MODULE WITH LOW-VOLUME HYBRIDIZATION CHAMBER AND LOW-VOLUME REAGENT RESERVOIR - A test module having an outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, a reagent reservoir containing a reagent for addition to the biological sample, and, a hybridization chamber containing probes having a nucleic acid sequence for hybridization with the target nucleic acid sequence to form probe-target hybrids, wherein, the reagent reservoir has a volume less than 20,000,000 cubic microns and the hybridization chamber has a volume less than 9,000 cubic microns. | 12-22-2011 |
20110312609 | TEST MODULE FOR ORIENTATION-INDEPENDENT OPERATION - A test module for analyzing genetic material in a biological sample, the test module having an outer casing with a receptacle for receiving the biological sample, functional sections for processing and analyzing the biological sample, a controller for operating the functional sections, and, a flow-path from the receptacle to the functional sections, wherein, the flow-path is configured to draw the sample to be analyzed from the receptacle to the functional sections by capillary action regardless of module orientation. | 12-22-2011 |
20110312610 | TEST MODULE WITH LONG FLUORESCENCE LIFETIME PROBES - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, and, probes in the outer casing for hybridization with the target nucleic acid sequence to form probe-target hybrids, the probes each having a fluorophore such that the probe-target hybrids emit a fluorescence signal in response to exposure to an excitation light, wherein, the fluorophore has a fluorescence lifetime greater than 100 nanoseconds. | 12-22-2011 |
20110312611 | TEST MODULE WITH TRANSITION METAL-LIGAND COMPLEX FLUOROPHORE - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, and, probes in the outer casing for hybridization with the target nucleic acid sequence to form probe-target hybrids, the probes each having a fluorophore such that the probe-target hybrids emit a fluorescence signal in response to exposure to an excitation light, wherein, the fluorophore is a transition metal-ligand complex. | 12-22-2011 |
20110312612 | LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET SEQUENCES WITH PROBES BETWEEN A WORKING ELECTRODE AND A PHOTOSENSOR - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, and, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, wherein, the electrodes are arranged in pairs, one of the electrodes in each electrode pair being a working electrode which causes oxidation or reduction of the luminophore to generate an excited species that emits a photon, the working electrode being positioned such that the probes are between the photosensor and the working electrode. | 12-22-2011 |
20110312615 | MICROFLUIDIC DEVICE WITH PARALLEL NUCLEIC ACID AMPLIFICATION SECTION - A microfluidic device for amplifying nucleic acid sequences, the microfluidic device having an inlet for receiving a sample containing genetic material, a plurality of reagent reservoirs containing reagents for addition to the sample, a first nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material in parallel with the first nucleic acid amplification section. | 12-22-2011 |
20110312616 | TEST MODULE WITH PARALLEL DNA AND RNA AMPLIFICATION SECTIONS - A test module for amplifying DNA and RNA, the test module having an outer casing with receptacle for receiving a sample containing genetic material including DNA and RNA, a plurality of reagent reservoirs containing reagents for addition to the sample, a first nucleic acid amplification section for amplifying at least some of the genetic material, and, a second nucleic acid amplification section for amplifying at least some of the genetic material in parallel with the first nucleic acid amplification section. | 12-22-2011 |
20110312617 | MONOLITHIC MICROSYSTEMS TECHNOLOGY DEVICE FOR OLIGONUCLEOTIDE SPOTTING - A microsystems technology (MST) device for contactless spotting of oligonucleotide probes onto a surface, the probes having nucleic acid sequences that are complementary to target nucleic acid sequences to be identified in a biological sample, the MST device having a monolithic substrate having a reservoir side and an ejector side opposite the reservoir side, an array of reservoirs formed in the reservoir side, and, an array of ejectors formed on the ejector side, each of the ejectors being configured for fluid communication with a corresponding one of the reservoirs respectively, wherein, the ejectors are configured to eject droplets containing the oligonucleotide probes from the corresponding reservoir onto the surface. | 12-22-2011 |
20110312618 | OLIGONUCLEOTIDE SPOTTING DEVICE WITH HIGH SPOTTING RATE - An oligonucleotide spotting device for contactless spotting of probes onto a surface, the probes having nucleic acid sequences that are complementary to target nucleic acid sequences to be identified in a biological sample, the oligonucleotide spotting device having an array of ejectors, each having an actuator for ejecting droplets of liquid containing the probes, CMOS circuitry for providing each of the actuators with a drive pulse for droplet ejection, wherein during use, the array of ejectors spot the probes onto the surface at a rate greater than 100 probe spots per second. | 12-22-2011 |
20110312619 | DEVICE FOR HIGH-DENSITY DEPOSITION OF BIOCHEMICALS - A biochemical deposition device for contactless deposition of biochemicals on a surface, the biochemical deposition device having a supporting substrate, an array of reservoirs on one side of the substrate, the reservoirs being configured for containing a plurality of biochemicals, and, an array of ejectors on the other side of the supporting substrate, the ejectors being in fluid communication with the reservoirs, and configured to eject droplets containing the biochemicals onto the surface with a density greater than 1 droplet per square millimeter. | 12-22-2011 |
20110312620 | SYSTEM FOR VARIABLE MICROARRAY SPOTTING AND GENETIC ANALYSIS - A system for microarray spotting and genetic analysis, the system having containers with probes for hybridization with different target nucleic acid sequences, an integrated circuit secured to each of the containers respectively, each of the integrated circuits have a container digital memory storing probe specification data related to probes in that container, a microfluidic device for supporting an array of probes selected from the containers, the selected probes corresponding to a desired genetic test assay, the microfluidic device having a device digital memory, an oligonucleotide spotting robot for spotting the selected probes onto the microfluidic device to form the array of probes, the oligonucleotide spotting robot having a control microprocessor for downloading the specification data to the device digital memory, and, a device reader for accessing the specification data from the device digital memory in order to analyze hybridization data from the microfluidic device. | 12-22-2011 |
20110312621 | APPARATUS FOR DISPENSING REAGENTS AND LOADING OLIGONUCLEOTIDE SPOTTING DEVICES - An apparatus for dispensing reagents and loading oligonucleotide spotting devices, the apparatus having a plurality of reagent vials, each with a droplet dispenser, a plurality of oligonucleotide vials, each with a droplet dispenser, a mounting surface for detachably mounting a microfluidic device for movement relative to the reagent vials, and detachably mounting an oligonucleotide spotting device, and, a control processor for operative control of the reagent vials and oligonucleotide vials and movement of the mounting surface relative to the reagent vials and oligonucleotide vials, wherein, the control processor is configured to activate any of the droplet dispensers, move the microfluidic device into registration with the reagent vials and move the oligonucleotide spotting device into registration with the oligonucleotide vials. | 12-22-2011 |
20110312622 | MICROFLUIDIC DEVICE WITH LOW-VOLUME HYBRIDIZATION CHAMBERS FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET SEQUENCES - A microfluidic device for detecting target nucleic acid sequences in a sample, the microfluidic device having an array of hybridization chambers, each containing electrochemiluminescent (ECL) probes for hybridization with the target nucleic acid sequences, and electrode pairs for receiving an electrical pulse, the ECL probes being configured to emit a photon of light when hybridized with one of the nucleic acid targets and activated by one of the electrodes, wherein, the hybridization chambers each have a volume less than 900000 cubic microns. | 12-22-2011 |
20110312623 | LOC DEVICE FOR PATHOGEN DETECTION WITH DIALYSIS, THERMAL LYSIS, NUCLEIC ACID AMPLIFICATION AND PREHYBRIDIZATION FILTERING - A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a first dialysis section for separating pathogens from larger constituents in the sample, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, the lysis section having a lysis chamber and a heater for lysing the pathogens while the sample is in the lysis chamber, a nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material, and, a second dialysis section downstream of the nucleic acid amplification section for prehybridization filtration of amplicon produced by the nucleic acid amplification section, the second dialysis section being configured to remove cell debris from the amplicon, wherein, the first dialysis section, the lysis section, the nucleic acid amplification section and the second dialysis section are all supported on the supporting substrate. | 12-22-2011 |
20110312624 | MICROFLUIDIC DEVICE WITH FLOW-CHANNEL STRUCTURE HAVING ACTIVE VALVE FOR CAPILLARY-DRIVEN FLUIDIC PROPULSION WITHOUT TRAPPED AIR BUBBLES - A microfluidic device for processing a fluid sample, the microfluidic device having a first channel configured to fill with the sample by capillary action, a second channel configured to fill with the sample by capillary action, a plurality of fluid connections between the first channel and the second channel, one of the fluid connections having an active valve to arrest the sample flow into the second channel and the remaining fluid connections each being configured to pin a meniscus of the sample that arrests capillary flow between the first channel and the second channel, the fluid connection with the active valve being upstream of the remaining fluid connections, wherein during use, the sample flow into the second channel is initiated by activation of the active valve such that the sample flow in the second channel progressively removes the meniscus at each of the meniscus anchors. | 12-22-2011 |
20110312625 | LOC DEVICE FOR GENETIC ANALYSIS WITH DIALYSIS, CHEMICAL LYSIS AND TANDEM NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating cells larger than a predetermined threshold in the sample from smaller constituents, whereby the cells larger than a predetermined threshold include target cells containing genetic material for analysis, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the target cells to release the genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the target cells in the lysis section, a first nucleic acid amplification section downstream of the lysis section for amplifying first nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section downstream of the first nucleic acid amplification section for amplifying second nucleic acid sequences in the amplicon from the first nucleic acid amplification section, wherein, the dialysis section, the lysis section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312626 | TEST MODULE INCORPORATING SPECTROMETER - A test module having an outer casing having a receptacle for receiving a biological sample, an excitation source mounted in the outer casing for sequentially illuminating the biological sample with different wavelengths of light, and, a photosensor positioned to detect the different wavelengths of light transmitted through the biological sample, wherein during use, the photosensor output signal is used to generate a spectrogram for analysis of a characteristic of the biological sample. | 12-22-2011 |
20110312627 | TEST MODULE WITH MICROFLUIDIC DEVICE HAVING LOC AND DIALYSIS DEVICE FOR SEPARATING PATHOGENS FROM OTHER CONSTITUENTS IN A BIOLOGICAL SAMPLE - A test module for concentrating pathogens in a biological sample, the test module having an outer casing with a receptacle for receiving the sample, a dialysis device in fluid communication with the receptacle and configured to separate the pathogens from other constituents in the sample, and, a lab-on-a-chip (LOC) device being in fluid communication with the dialysis device and configured to analyze the pathogens. | 12-22-2011 |
20110312628 | MICROFLUIDIC DEVICE WITH MST LAYER AND OVERLYING CAP - A microfluidic device having a supporting substrate, a microsystems technologies (MST) layer on the supporting substrate, a cap overlying the MST layer, the cap having a plurality of fluidic connections between the cap and the MST layer for fluid flow from the MST layer to the cap and fluid flow from the cap to the MST layer. | 12-22-2011 |
20110312629 | MICROFLUIDIC DEVICE WITH DIALYSIS SECTION HAVING STOMATA TAPERING COUNTER TO FLOW DIRECTION - A microfluidic device for removing cell debris from a biological sample, the microfluidic device having a dialysis section with a large constituent channel and a small constituent channel, and a series of stoma for fluid communication between the large constituent channel and the small constituent channel, the large constituent channel having an upstream end for receiving the biological sample, the biological sample being a liquid carrying a mixture of cell debris and target molecules, the small constituent channel having a downstream end for connection to a hybridization section with an array of probes for reaction with the target molecules to form probe-target complexes, wherein, each of the stoma is tapered in a counter-flow direction such that each have a small opening to the large constituent channel and a large opening to the small constituent channel, the small openings being sized to allow the target molecules to flow into the small constituent channel but retain the cell debris larger than a threshold size in the large constituent channel. | 12-22-2011 |
20110312630 | LOC DEVICE FOR PATHOGEN DETECTION, GENETIC ANALYSIS AND PROTEOMIC ANALYSIS WITH DIALYSIS, CHEMICAL LYSIS, INCUBATION AND TANDEM NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for pathogen detection, genetic analysis and proteomic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a leukocyte dialysis section for dividing the sample into a leukocyte stream, and a pathogen and erythrocyte stream containing cells and sample constituents smaller than a first size threshold, a pathogen dialysis section for dividing the pathogen and erythrocyte stream into an erythrocyte stream and a pathogen stream containing cells and sample constituents smaller than a second size threshold, a leukocyte lysis section downstream of the leukocyte dialysis section for lysing the leukocytes with a lysis reagent to release genetic material and proteins therein, a leukocyte incubation section downstream of the leukocyte lysis section for enzymatic reaction of the genetic material with enzymes, a pathogen lysis section downstream of the pathogen dialysis section for lysing the pathogens with a lysis reagent to release genetic material and proteins therein, a pathogen incubation section downstream of the pathogen lysis section for enzymatic reaction of the genetic material with enzymes, an erythrocyte lysis section downstream of the pathogen dialysis section for lysing the erythrocytes with a lysis reagent to release proteins therein, a first leukocyte nucleic acid amplification section downstream of the leukocyte incubation section for amplifying nucleic acid sequences, a second leukocyte nucleic acid amplification section downstream of the first leukocyte nucleic acid amplification section for amplifying nucleic acid sequences, a first pathogen nucleic acid amplification section downstream of the pathogen incubation section for amplifying nucleic acid sequences, and, a second pathogen nucleic acid amplification section downstream of the first pathogen nucleic acid amplification for amplifying nucleic acid sequences, wherein, the leukocyte dialysis section, the pathogen dialysis section, the leukocyte lysis section, the pathogen lysis section, the leukocyte incubation section, the pathogen incubation section, the first and second leukocyte nucleic acid amplification sections and the first and second pathogen nucleic acid amplification sections are all supported on the supporting substrate. | 12-22-2011 |
20110312631 | GENETIC ANALYSIS LOC WITH NON-SPECIFIC NUCLEIC ACID AMPLIFICATION SECTION AND SUBSEQUENT SPECIFIC AMPLIFICATION OF PARTICULAR SEQUENCES IN A SEPARATE SECTION - A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing dNTP's, primers, polymerase and buffer solution for addition to the sample, a first nucleic acid amplification section for thermal control of the sample to amplify the target nucleic acid sequences, and, a second nucleic acid amplification section for thermal control of amplicon from the first nucleic acid amplification section to further amplify the target nucleic acid sequences. | 12-22-2011 |
20110312632 | MICROFLUIDIC DEVICE WITH PCR SECTION AND DIFFUSION MIXER - A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, a reagent reservoir containing a reagent, and, a diffusion mixing section for mixing the nucleic acid sequences with the reagent, the diffusion mixing section having a microchannel defining a tortuous flow-path having a length sufficient for diffusive mixing of the reagent and the sample, wherein during use, the sample flows from the sample inlet to the PCR section via the diffusion mixing section. | 12-22-2011 |
20110312633 | MICROFLUIDIC DEVICE WITH DIALYSIS SECTION - A microfluidic device for dialysis of biological material, the microfluidic device having a sample inlet for receiving a sample of biological material that has constituents of different sizes, a dialysis section for separating smaller constituents from larger constituents, the smaller constituents being smaller than a predetermined size threshold, and the larger constituents being larger than the predetermined size threshold, and, a microsystems technology (MST) layer for separately processing the smaller constituents and/or the larger constituents. | 12-22-2011 |
20110312634 | MICROFLUIDIC DEVICE WITH LAMINAR STRUCTURE - A microfluidic device having a supporting substrate, a microsystems technology (MST) layer overlying the supporting substrate, the MST layer defining MST channels for fluid flow within the MST layer, a cap overlying the MST layer, the cap defining cap channels for fluid flow within the cap and reservoirs for holding a quantity of fluid, wherein, the MST channels and the cap channels are in fluid communication. | 12-22-2011 |
20110312635 | MICROFLUIDIC DEVICE WITH FLOW-CHANNEL STRUCTURE FOR CAPILLARY-DRIVEN FLUIDIC PROPULSION WITHOUT TRAPPED AIR BUBBLES - A microfluidic device for processing a fluid sample, the microfluidic device having a first channel configured to fill with the sample by capillary action, a second channel configured to fill with the sample by capillary action, a plurality of fluid connections between the first channel and the second channel, each of the fluid connections being configured to pin a meniscus of the sample that arrests capillary flow between the first channel and the second channel, a bypass channel between the first channel and the second channel, the bypass channel joining the second channel upstream of the plurality of fluid connections and is configured for uninterrupted capillary driven flow from the first channel to the second channel, wherein during use, flow from the bypass channel reaches the meniscus pinned at each of the fluid connections after the meniscus has formed such that the flow sequentially removes each of the menisci and sample flow from the first channel to the second channel is via the plurality of fluid connections as well as the bypass channel. | 12-22-2011 |
20110312636 | LOC DEVICE WITH DIALYSIS SECTION FOR SEPARATING LEUKOCYTES FROM BLOOD - A lab-on-a-chip (LOC) device to for analyzing leukocytes in a blood sample, the LOC device having a supporting substrate, an inlet for receiving a sample of blood including leukocytes and erythrocytes, a dialysis section for separating at least some of the erythrocytes from the leukocytes, and, a microsystems technology (MST) layer for separately analyzing the leukocytes, wherein, the inlet, the dialysis section and the MST layer are all supported on the supporting substrate. | 12-22-2011 |
20110312637 | LOC DEVICE WITH DIALYSIS SECTION FOR SEPARATING PATHOGENS FROM A BIOLOGICAL SAMPLE - A lab-on-a-chip (LOC) device to separate pathogens from a biological sample for analysis, the LOC device having a supporting substrate, an inlet for receiving the sample including pathogens and other constituents larger than the pathogens, a dialysis section for separating the pathogens into a portion of the sample, and, a microsystems technology (MST) layer for separately analyzing the pathogens, wherein, the inlet, the dialysis section and the MST layer are all supported on the supporting substrate. | 12-22-2011 |
20110312638 | LOC DEVICE FOR PATHOGEN DETECTION AND GENETIC ANALYSIS WITH DIALYSIS AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for pathogen detection and genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a plurality of reagent reservoirs, a dialysis section for separating pathogens and cells larger than a predetermined threshold in the sample from smaller constituents, whereby the pathogens and cells larger than the predetermined threshold contain genetic material for analysis, a nucleic acid amplification section downstream of the dialysis section for amplifying nucleic acid sequences from the genetic material, wherein, the dialysis section and the nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312639 | LOC DEVICE WITH DIALYSIS SECTION FOR SEPARATING LEUKOCYTES AND PATHOGENS FROM BLOOD - A lab-on-a-chip (LOC) device to separate pathogens and leukocytes from a blood sample for analysis, the LOC device having a sample inlet for receiving the blood including pathogens, leukocytes and other constituents, a dialysis section for separating the pathogens into a first portion of the sample and separating the leukocytes into a second portion of the sample such that the remainder of the sample has constituents smaller than the leukocytes and larger than the pathogens, and, a microsystems technology (MST) layer for analyzing the pathogens and the leukocytes. | 12-22-2011 |
20110312640 | MICROFLUIDIC DEVICE WITH PHOTOSENSOR - A microfluidic device having a supporting substrate, a microsystems technologies (MST) layer for processing a fluid sample, and, a photosensor adjacent the MST layer for detecting photons emitted from the MST layer. | 12-22-2011 |
20110312641 | MICROFLUIDIC DEVICE WITH SAMPLE INLET AND PROBE HYBRIDIZATION SECTION - A microfluidic device for detecting hybridization of probes with target nucleic acid sequences, the microfluidic device having a sample inlet for receiving a fluid sample with target nucleic acid sequences, an array of probes for hybridization with the target nucleic acid sequences, and, a flow-path from the sample inlet to the array, wherein, the flow-path is configured to draw the fluid sample from the sample inlet to the probes by capillary action. | 12-22-2011 |
20110312642 | MICROFLUIDIC DEVICE FOR DETECTION OF NUCLEIC ACID TARGETS WITH ELECTROCHEMILUMINESCENT PROBES - A microfluidic device for detecting a target nucleic acid sequence in a sample, the microfluidic device having a sample inlet for receiving the sample, probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, and, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, wherein, the sample inlet draws the sample along a fluid flow-path leading to the probes by capillary action. | 12-22-2011 |
20110312643 | MICROFLUIDIC DEVICE FOR DETECTION OF HYBRIDIZATION OF NUCLEIC ACID TARGETS - A microfluidic device having a supporting substrate, a microsystems technologies (MST) layer with a hybridization section that has an array of probes for hybridization with target nucleic acid sequences to form probe-target hybrids, and, a photosensor for detecting the probe-target hybrids. | 12-22-2011 |
20110312644 | MICROFLUIDIC DEVICE FOR SIMULTANEOUS DETECTION OF MULTIPLE CONDITIONS IN A PATIENT - A microfluidic device for simultaneous detection of multiple conditions in a patient, the microfluidic device having an inlet for receiving a sample of biological material drawn from the patient, a microsystems technologies (MST) layer with a detection section having an array of probes for reaction with target molecules in the sample to form probe-target complexes, the target molecules being indicative of medical conditions in the patient, and, a photosensor for detecting the probe-target complexes, wherein, the array of probes has more than 1000 probes. | 12-22-2011 |
20110312645 | MICROFLUIDIC DEVICE WITH TEMPERATURE FEEDBACK CONTROLLED HYBRIDIZATION CHAMBERS FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGETS - A microfluidic device for detecting target nucleic acid sequences in a sample, the microfluidic device having a supporting substrate, a microsystems technology (MST) layer overlying the supporting substrate for processing the sample, the MST layer having an array of hybridization chambers, each containing electrochemiluminescent (ECL) probes for hybridization with the target nucleic acid sequences, and electrode pairs for receiving an electrical pulse, the ECL probes being configured to emit a photon of light when hybridized with one of the nucleic acid targets and activated by one of the electrodes, an array of temperature sensors positioned such that at least one of the temperature sensors corresponds to each of the hybridization chambers respectively, and, heaters for heating each of the hybridization chambers, such that, output from the temperature sensors is used for feedback control of the heaters. | 12-22-2011 |
20110312646 | LOC DEVICE FOR SEPARATING CONSTITUENTS OF INTERMEDIATE SIZE FROM LARGER AND SMALLER CONSTITUENTS IN A BIOLOGICAL SAMPLE - A lab-on-a-chip (LOC) device to separate constituents of intermediate size from larger and smaller constituents in a biological sample, the LOC device having an inlet for receiving the biological sample including large constituents sized above an upper threshold, small constituents sized smaller than a lower threshold and intermediate constituents sized between the upper and the lower thresholds, a first channel in fluid communication with the inlet, first apertures sized to correspond to the upper threshold, a second channel in fluid communication with the first channel via the first apertures, second apertures sized to correspond to the lower threshold, a third channel in fluid communication with the second channel via the second apertures, and, a microsystems technology (MST) layer for analyzing the large, intermediate and or small constituents separately, wherein during use, the intermediate and the small constituents flow into the second channel via the first apertures while the large constituents are retained in the first channel, and the small constituents flow into the third channel while the intermediate constituents are retained in the second channel | 12-22-2011 |
20110312647 | MICROFLUIDIC DEVICE WITH TEMPERATURE FEEDBACK CONTROLLED HYBRIDIZATION CHAMBERS - A microfluidic device having a supporting substrate, a microsystems technology (MST) layer overlying the supporting substrate for processing a fluid containing target nucleic acid sequences, the MST layer having an array of hybridization chambers, each containing probes for hybridization with the target nucleic acid sequences, a temperature sensor for sensing the temperature at the array of hybridization chambers, and, a hybridization heater for heating the array of hybridization chambers, wherein during use, output from the temperature sensor is used for feedback control of the hybridization heater. | 12-22-2011 |
20110312648 | MICROFLUIDIC DEVICE FOR GENETIC AND MITOCHONDRIAL ANALYSIS OF A BIOLOGICAL SAMPLE - A microfluidic device for analysis of genetic DNA and mitochondrial DNA in a sample, the microfluidic device having an inlet for receiving a sample of biological material having cells with an outer cellular membrane containing mitochondria and a nucleus, the mitochondria has a mitochondrial membrane containing mitochondrial DNA and the nuclei have a nuclear membrane containing nuclear DNA, a first lysis section for lysing the outer cellular membrane and the mitochondrial membrane while the nuclear membrane remains intact, a second lysis section for lysing the nuclear membrane, mitochondrial probes downstream of the first lysis section for hybridization with target nucleic acid sequences in the mitochondrial DNA to form probe-target hybrids, and, nuclear probes downstream of the second lysis second for hybridization with target nucleic acid sequences in the nuclear DNA to form probe-target hybrids. | 12-22-2011 |
20110312649 | MICROFLUIDIC DEVICE WITH OPTICALLY TRANSPARENT HYBRIDIZATION CHAMBERS - A microfluidic device having a supporting substrate, a microsystems technology (MST) layer overlying the supporting substrate for processing a fluid containing target nucleic acid sequences, the MST layer having an array of hybridization chambers, each containing probes for hybridization with the target nucleic acid sequences, the probes being configured to emit a fluorescence signal in response to an excitation light upon hybridization, wherein, the hybridization chamber has a wall section that is optically transparent to the fluorescence signal. | 12-22-2011 |
20110312650 | MICROFLUIDIC DEVICE WITH OPTICALLY TRANSPARENT HYBRIDIZATION CHAMBERS FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGETS - A microfluidic device for detecting target nucleic acid sequences in a sample, the microfluidic device having an array of hybridization chambers, each containing electrochemiluminescent (ECL) probes for hybridization with the target nucleic acid sequences, and electrode pairs for receiving an electrical pulse, the ECL probes being configured to emit a photon of light when hybridized with one of the nucleic acid targets and activated by one of the electrodes, wherein, the hybridization chambers each have a wall section that is optically transparent to the light emitted by the ECL probes. | 12-22-2011 |
20110312651 | MICROFLUIDIC DEVICE WITH LOW MASS PROBE SPOTS - A microfluidic device having a supporting substrate, a microsystems technology (MST) layer overlying the supporting substrate for processing a fluid containing target nucleic acid sequences, the MST layer having a plurality of probe spots, each of the probe spots containing probes for hybridization with the target nucleic acid sequences, wherein, the mass of the probes in each of the probe spots is less than 270 picograms. | 12-22-2011 |
20110312652 | MICROFLUIDIC DEVICE WITH LOW-VOLUME ELECTROCHEMILUMINESCENCE-BASED PROBE SPOTS - A microfluidic device for detecting target nucleic acid sequences in a sample, the microfluidic device having an array of hybridization chambers, each of the hybridization chambers containing electrode pairs for receiving an electrical pulse and electrochemiluminescent (ECL) probes for hybridization with the target nucleic acid sequences, the ECL probes being configured to emit a photon of light when hybridized with one of the nucleic acid targets and excited by current between the electrodes, wherein, the mass of the probes in each of the hybridization chambers is less than 270 picograms. | 12-22-2011 |
20110312653 | MICROFLUIDIC DEVICE WITH LOW-VOLUME HYBRIDIZATION CHAMBERS - A microfluidic device having a supporting substrate, a microsystems technologies (MST) layer with a hybridization section that has an array of hybridization chambers, each containing a different probe type for hybridization with target nucleic acid sequences, wherein, each of the hybridization chambers has a volume less than 900,000 cubic microns. | 12-22-2011 |
20110312654 | APPARATUS FOR LOADING OLIGONUCLEOTIDE SPOTTING DEVICES AND SPOTTING OLIGONUCLEOTIDE PROBES - An apparatus for loading oligonucleotide spotting devices and spotting oligonucleotide probes, the apparatus having a plurality of oligonucleotide vials, each with a droplet dispenser, a mounting surface for detachably mounting an oligonucleotide spotting device, a chuck for detachably mounting the oligonucleotide spotting device adjacent the mounting surface, and, a control processor for operative control of the oligonucleotide vials, the oligonucleotide spotting device when mounted in the chuck and movement of the mounting surface relative to the oligonucleotide vials, and the oligonucleotide spotting device, wherein, the control processor is configured to activate the droplet dispensers, and move the oligonucleotide spotting device into registration with the oligonucleotide vials. | 12-22-2011 |
20110312655 | MICROFLUIDIC DEVICE FOR PCR, PROBE HYBRIDIZATION AND ELECTROCHEMILUMINESCENT DETECTION OF PROBE-TARGET HYBRIDS - A microfluidic device for amplifying and detecting target nucleic acid sequences in a sample, the microfluidic device having a polymerase chain reaction (PCR) section for amplifying the target nucleic acid sequences, and, a hybridization section that has an array of electrochemiluminescent (ECL) probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, and a plurality of electrodes for receiving an electrical pulse such that the probe-target hybrids emit a photon of light. | 12-22-2011 |
20110312656 | MICROFLUIDIC DEVICE FOR PCR AND PROBE HYBRIDIZATION - A microfluidic device having a sample inlet for receiving a sample of biological material containing target nucleic acid sequences, and, a microsystems technologies (MST) layer with a polymerase chain reaction (PCR) section for amplifying the target nucleic acid sequences, and an array of probes for hybridization with the target nucleic acid sequences. | 12-22-2011 |
20110312657 | MICROFLUIDIC TEST MODULE FOR INTERFACING WITH A MOBILE TELEPHONE - A microfluidic test module for analyzing a sample fluid and communicating test results to a mobile telephone, the microfluidic test module having an outer casing with a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the mobile telephone, and, a controller for operative control of the communication interface. | 12-22-2011 |
20110312658 | LOC DEVICE WITH DIALYSIS SECTION FOR CONCENTRATING NUCLEATED CELLS IN A BIOLOGICAL SAMPLE - A lab-on-a-chip (LOC) device for concentrating nucleated cells in a biological sample, the LOC device having an inlet for receiving a sample of biological material including nucleated cells and other constituents, the nucleated cells all being larger than a predetermined threshold, a dialysis section for concentrating the nucleated cells into a portion of the sample such that the remainder of the sample contains the other constituents smaller than the predetermined threshold, and, a microsystems technology (MST) layer for processing the nucleated cells. | 12-22-2011 |
20110312659 | MICROFLUIDIC DEVICE WITH HYBRIDIZATION CHAMBERS AND CORRESPONDING DIFFUSION BARRIERS - A microfluidic device having a sample flow-path for a biological sample containing target nucleic acid sequences, an array of hybridization chambers each containing probes for hybridization with the target nucleic acid sequences, each of the hybridization chambers having a chamber inlet for fluid communication between the sample flow-path and the probes, wherein, the chamber inlet is configured as a diffusion barrier to prevent diffusion of hybridized probes between the hybridization chambers to an extent that causes erroneous hybridization detection results. | 12-22-2011 |
20110312660 | MICROFLUIDIC TEST MODULE FOR INTERFACING WITH A LAPTOP COMPUTER - A microfluidic test module for analyzing a sample fluid and communicating test results to a laptop computer, the microfluidic test module having an outer casing with a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the laptop computer, and, a controller for operative control of the communication interface. | 12-22-2011 |
20110312661 | MICROFLUIDIC DEVICE WITH ARRAY OF CHAMBERS AND CORRESPONDING DIFFUSION BARRIERS FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGETS - A microfluidic device for detecting target molecules in a fluid, the microfluidic device having an array of chambers, each containing electrochemiluminescent (ECL) probes for reaction with the target molecules to form a probe-target complex, and electrodes positioned in each of the chambers for receiving an electrical pulse, the probe-target complexes being configured to emit a photon of light when excited by current between the electrodes, a flow-path for the fluid containing the targets, wherein, each of the chambers have a chamber inlet for fluid communication between the sample flow-path and the probes within the chamber, the chamber inlet being configured as a diffusion barrier to prevent diffusion of the probe-target complexes between the chambers to an extent that causes erroneous detection results. | 12-22-2011 |
20110312662 | LOC DEVICE WITH DIALYSIS SECTION FOR REMOVING CELL DEBRIS FROM A BIOLOGICAL SAMPLE - A lab-on-a-chip (LOC) device for removing cell debris from a biological sample, the LOC device having a dialysis section with a large constituent channel, a small constituent channel and a plurality of apertures for fluid communication between the large constituent channel and the small constituent channel, the large constituent channel having an upstream end for receiving the biological sample, the biological sample being a liquid carrying a mixture of cell debris and target molecules, the small constituent channel having a downstream end for connection to a hybridization section with an array of probes for reaction with the target molecules to form probe-target complexes, wherein, the apertures are sized to allow the target molecules to flow into the small constituent channel but retain the cell debris larger than a threshold size in the large constituent channel. | 12-22-2011 |
20110312663 | MICROFLUIDIC DEVICE WITH TIME DELAYED DETECTION OF FLUORESCENCE FROM HYBRIDIZED PROBES - A microfluidic device having a supporting substrate, a probe having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, CMOS circuitry on the substrate, the CMOS circuitry having a photosensor for generating an output signal in response to the fluorescence signal, wherein, the CMOS circuitry is configured to trigger a time delay when the excitation light is deactivated before activating the photosensor. | 12-22-2011 |
20110312664 | MICROFLUIDIC TEST MODULE FOR INTERFACING WITH A DEDICATED READER - A microfluidic test module for analyzing a sample fluid and communicating test results to a dedicated reader purpose built for operating with the microfluidic test module, the microfluidic test module having an outer casing with a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the dedicated reader, and, a controller for operative control of the communication interface. | 12-22-2011 |
20110312665 | LOC WITH DIALYSIS SECTION FOR REMOVING INSOLUBLE SAMPLE CONSTITUENTS FROM A NUCLEIC ACID MIXTURE - A lab-on-a-chip (LOC) device for removing cell debris from a biological sample containing target nucleic acid sequences, the LOC device having a dialysis section with a large constituent channel, a small constituent channel and a plurality of apertures for fluid communication between the large constituent channel and the small constituent channel, the large constituent channel having an upstream end for receiving the biological sample, the biological sample being a liquid carrying a mixture of cell debris and target nucleic acid sequences, and the small constituent channel having a downstream end for connection to a hybridization section with an array of probes for hybridization with the target nucleic acid sequences to form probe-target complexes, wherein, the apertures are sized to allow the target nucleic acid sequences to flow into the small constituent channel but retain the cell debris larger than a threshold size in the large constituent channel. | 12-22-2011 |
20110312666 | MICROFLUIDIC DEVICE WITH TRIGGERED PHOTODETECTION OF FLUORESCING PROBE-TARGET HYBRID - A microfluidic device having a probe for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid having a reporting fluorophore for emitting a fluorescence signal in response to an excitation light, a detection photodiode for exposure to the excitation light and the fluorescence signal, and, a trigger photodiode for exposure to the excitation light, the trigger photodiode being configured to activate the detection photodiode after the excitation light has been extinguished. | 12-22-2011 |
20110312667 | MICROFLUIDIC TEST MODULE FOR INTERFACING WITH A DESKTOP COMPUTER - A microfluidic test module for analyzing a sample fluid and communicating test results to a desktop computer, the microfluidic test module having an outer casing with a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the desktop computer, and, a controller for operative control of the communication interface. | 12-22-2011 |
20110312668 | LOC WITH DIALYSIS SECTION FOR RETAINING INSOLUBLE SAMPLE CONSTITUENTS AFTER AMPLIFICATION AND PASSING SOLUBLE CONSTITUENTS TO A DETECTION SECTION - A lab-on-a-chip (LOC) device for removing cell debris from a biological sample containing target nucleic acid sequences, the LOC device having an amplification section for amplifying the nucleic acid sequences, a dialysis section with a large constituent channel, a small constituent channel and a plurality of apertures for fluid communication between the large constituent channel and the small constituent channel, the large constituent channel configured to receive the biological sample after nucleic acid amplification in the amplification section, the biological sample being a liquid mixture of the cell debris and the target nucleic acid sequences and the apertures are sized to allow the target nucleic acid sequences to flow into the small constituent channel while the cell debris larger than a threshold size is retained in the large constituent channel, and, an array of probes in fluid communication with the small constituent channel for hybridization with the target nucleic acid sequences to form probe-target hybrids. | 12-22-2011 |
20110312669 | MICROFLUIDIC DEVICE WITH ELECTROCHEMILUMINESCENT PROBES AND PHOTOSENSOR WITH LARGE ANGLE OF COLLECTION FOR PROBE EMITTTED LIGHT - A microfluidic device for detecting target molecules in a fluid, the microfluidic device having a hybridization chamber containing electrodes and probes for hybridization with target molecules to form probe-target complexes and generate an electrochemiluminescence (ECL) signal in response to an electrical current between the electrodes, and, a photodiode with an active area and an optical axis extending normal to the active area and through the hybridization chamber, wherein, the hybridization chamber has a floor surface positioned parallel to the active area of the photodiode, the floor surface having a centroid and the active area being encompassed within a cone having the centroid at its vertex, and a vertex angle less than 174°. | 12-22-2011 |
20110312670 | MICROFLUIDIC TEST MODULE FOR INTERFACING WITH AN EBOOK READER - A microfluidic test module for analyzing a sample fluid and communicating test results to an ebook reader, the microfluidic test module having an outer casing with a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the ebook reader, and, a controller for operative control of the communication interface. | 12-22-2011 |
20110312671 | SINGLE USE MICROFLUIDIC DEVICE WITH PHOTOSENSOR FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGETS - A microfluidic device for detecting target molecules in a fluid, the microfluidic device having an array of electrochemiluminescent (ECL) probes for reaction with the target molecules to form a probe-target complexes, electrodes for receiving an electrical pulse, the probe-target complexes being configured to emit a photon of light when excited by current between the electrodes, and, a photosensor for detecting the light emitted by the probes, wherein during use, addition of the fluid to the probes prevents subsequent addition of other fluid to the probes. | 12-22-2011 |
20110312672 | MICROFLUIDIC ASSEMBLY WITH TEST MODULE AND DETACHABLE INDICATOR MODULE - A microfluidic assembly for detecting a target molecule in a sample fluid and indicating a positive or negative result, the microfluidic assembly having a test module having an outer casing with a receptacle for receiving the sample, functional sections for processing the sample and detecting the target molecules, a communication interface a controller for transmitting an output signal from one or more of the functional sections, and, an indicator module for detachable engagement with the communication interface, the indicator module having an indicator for providing an indication of the target molecule being present in the fluid sample. | 12-22-2011 |
20110312673 | DIALYSIS DEVICE WITH MULTI-LAYER STRUCTURE - A dialysis device for dialysis of a fluid containing constituents of different sizes, the dialysis device having a first layer of material defining a first channel and a second channel, the first channel configured for receiving the fluid which contains constituents of different sizes, a second layer having a plurality of apertures open to the first channel and at least one fluid connection leading from the apertures to the second channel for establishing fluid communication between the first channel and the second channel, wherein, the apertures are sized in accordance with a predetermined size threshold such that the constituents flowing to the second channel are smaller constituents that are smaller than the predetermined size threshold, and the constituents retained in the first channel include larger constituents which are larger than the predetermined size threshold. | 12-22-2011 |
20110312674 | LOC DEVICE WITH INTEGRAL PHOTOSENSOR FOR ELECTROCHEMILUMINESCENCE BASED DETECTION OF TARGETS - A lab-on-a-chip (LOC) device for detecting target molecules in a fluid, the LOC device having an array of electrochemiluminescent (ECL) probes for reaction with the target molecules to form probe-target complexes, electrodes for receiving an electrical pulse, the probe-target complexes being configured to emit a photon of light when excited by current between the electrodes, and, a photosensor for detecting light emitted by the probe-target complexes. | 12-22-2011 |
20110312675 | DIALYSIS DEVICE WITH FLOW-CHANNEL STRUCTURE FOR CAPILLARY-DRIVEN FLUIDIC PROPULSION WITHOUT TRAPPED AIR BUBBLES - A dialysis device with capillary-driven, flow-channel structure for dialysis of a fluid containing constituents of different sizes, the dialysis device having a first channel configured to fill with the fluid by capillary action, a second channel configured to fill with the fluid by capillary action, a plurality of fluid connections between the first channel and the second channel, each of the fluid connections being configured to pin a meniscus of the fluid that arrests capillary flow between the first channel and the second channel, a bypass channel between the first channel and the second channel, the bypass channel joining the second channel upstream of the plurality of fluid connections and is configured for uninterrupted capillary driven flow from the first channel to the second channel, wherein during use, flow from the bypass channel reaches the meniscus pinned at each of the fluid connections after the meniscus has formed such that the flow sequentially removes each of the menisci and sample flow from the first channel to the second channel is via the plurality of fluid connections as well as the bypass channel. | 12-22-2011 |
20110312676 | LOC DEVICE WITH INTEGRAL DRIVER FOR EXCITATION OF ELECTROCHEMILUMINESCENT LUMINOPHORES - A lab-on-a-chip (LOC) device for exciting electrochemiluminescent luminophores, the LOC device having a supporting substrate, an array of electrochemiluminescent (ECL) probes for reaction with the target molecules to form probe-target complexes, the probes each having a luminophore for emitting photons of light when in an excited state, electrodes for receiving an electrical pulse to excite the luminophores with current between the electrodes, and, CMOS circuitry for controlling the electrical pulse transmitted to the electrodes, wherein, the CMOS circuitry is between the supporting substrate and the array of probes. | 12-22-2011 |
20110312677 | LOC DEVICE FOR DETECTION OF TARGETS WITH ELECTROCHEMILUMINESCENT RESONANT ENERGY TRANSFER PROBES - A lab-on-a-chip (LOC) device for detecting target molecules in a fluid, the LOC device having an array of electrochemiluminescent (ECL) probes for reaction with the target molecules to form probe-target complexes, and, electrodes for receiving an electrical pulse, wherein, the probes each have an ECL luminophore that emits a photon when excited by current between the electrodes, and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer. | 12-22-2011 |
20110312678 | TEST MODULE WITH MICROFLUIDIC DEVICE HAVING DIALYSIS DEVICE, LOC AND INTERCONNECTING CAP - A test module for analyzing a sample fluid containing target molecules, the test module having an outer casing with a receptacle for receiving the sample fluid, and, a microfluidic device having: a dialysis device in fluid communication with the receptacle and configured to separate the target molecules from other constituents of the sample, a lab-on-a-chip (LOC) device for analyzing the target molecules, and, a cap overlaying the LOC device and the dialysis device for establishing fluid communication between the LOC device and the dialysis device. | 12-22-2011 |
20110312679 | MICROFLUIDIC DEVICE WITH SURFACE-MICROMACHINED DIALYSIS SECTION - A microfluidic device for removing cell debris from a biological sample, the microfluidic device having a dialysis section with a large constituent channel, a small constituent channel and a plurality of apertures for fluid communication between the large constituent channel and the small constituent channel, the large constituent channel having an upstream end for receiving the biological sample, the biological sample being a liquid carrying a mixture of cell debris and target molecules, the small constituent channel having a downstream end for connection to a hybridization section with an array of probes for reaction with the target molecules to form probe-target complexes, wherein, the apertures are spaced along the large constituent channel at a spacing between 1 micron and 10 microns and each of the apertures is sized to allow the target molecules to flow into the small constituent channel but retain the cell debris larger than a threshold size in the large constituent channel. | 12-22-2011 |
20110312680 | LOC DEVICE FOR DETECTING HYBRIDIZATION OF TARGET NUCLEIC ACID SEQUENCES WITH ELECTROCHEMILUMINESCENT RESONANT ENERGY TRANSFER, PRIMER-LINKED, LINEAR PROBES - A lab-on-a-chip (LOC) device for detecting hybridization of target nucleic acid sequences, the LOC device having electrochemiluminescent (ECL), resonant energy transfer, primer-linked, linear probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, each of the probes having a linear portion containing the target nucleic acid sequence, a primer for extension along the target nucleic acid sequence to form a nucleic acid sequence complementary to the target, an ECL luminophore for emitting photons when in an excited state, and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and, electrodes for receiving an electrical pulse to excite the ECL luminophores, wherein during use, replicating the target nucleic acid sequence causes the linear portion to dissociate from the functional moiety such that the complementary nucleic acid sequence therein hybridizes to the target nucleic acid sequence and photons emitted by the ECL luminophore are not quenched. | 12-22-2011 |
20110312681 | LOC DEVICE WITH DIALYSIS SECTION FOR REMOVING ERYTHROCYTES FROM BLOOD - A lab-on-a-chip (LOC) device for analyzing leukocytes in a blood sample, the LOC device having a supporting substrate, an inlet for receiving a sample of blood, a dialysis section for separating erythrocytes from leukocytes in the blood, and, a microsystems technology (MST) layer for analyzing the leukocytes, wherein, the inlet, the dialysis section and the MST layer are all supported on the supporting substrate. | 12-22-2011 |
20110312682 | LOC DEVICE FOR AMPLIFYING AND DETECTING TARGET NUCLEIC ACID SEQUENCES USING ELECTROCHEMILUMINESCENT RESONANT ENERGY TRANSFER, STEM-AND-LOOP PROBES WITH COVALENTLY ATTACHED PRIMERS - A lab-on-a-chip (LOC) device for amplifying and detecting target nucleic acid sequences, the LOC device having electrochemiluminescent (ECL), resonant energy transfer, stem-and-loop probes for hybridization with the target nucleic acid sequences, each of the probes having a loop portion containing a sequence complementary to the target nucleic acid sequence, an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and a covalently attached primer for extension along a complementary sequence denatured from the target nucleic acid sequence to replicate the target nucleic acid sequence, heaters for thermally cycling the target nucleic acid sequences through a polymerase chain reaction (PCR), in which the covalently attached primers anneal to oligonucleotides containing the target nucleic acid sequences, and, electrodes for receiving an electrical pulse to excite the ECL luminophores, wherein during use, replicating the target nucleic acid sequence causes the loop portion to open such the complementary nucleic acid sequence therein hybridizes to the target nucleic acid sequence and the ECL luminophore is moved away from the functional moiety. | 12-22-2011 |
20110312683 | MICROFLUIDIC TEST MODULE FOR INTERFACING WITH TABLET COMPUTER - A microfluidic test module for analyzing a sample fluid and communicating test results to a tablet computer, the microfluidic test module having an outer casing with a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the tablet computer, and, a controller for operative control of the communication interface. | 12-22-2011 |
20110312684 | LOC DEVICE FOR PATHOGEN DETECTION WITH DIALYSIS, LYSIS AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, and, a nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material, wherein, the dialysis section, the lysis section and the nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312685 | LOC DEVICE FOR PCR USING ADAPTOR PRIMERS AND TARGET DETECTION USING ELECTROCHEMILUMINESCENT RESONANT ENERGY TRANSFER PROBES - A lab-on-a-chip (LOC) device for amplifying and detecting target nucleic acid sequences, the LOC device having restriction enzymes for restricting double stranded oligonucleotides at known ligation sites, linker molecules for attaching to the ligated ends of the double stranded oligonucleotides, heaters for thermally cycling the oligonucleotides through a polymerase chain reaction (PCR) process, primers for annealing to the linker molecules on single strands of the oligonucleotides after denaturation, deoxyribonucleoside triphosphates (dNTPs) for extending the primers along the single strand oligonucleotides, and, electrodes for receiving an electrical pulse to excite the ECL luminophores. | 12-22-2011 |
20110312686 | MICROFLUIDIC DEVICE WITH ELONGATE PCR CHAMBERS - A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having at least one elongate PCR chamber having a longitudinal extent much greater than its lateral dimensions, and, at least one elongate heater element for heating the nucleic acid sequences within the elongate PCR chamber, wherein, the elongate heater element extends parallel with the longitudinal extent of the PCR chamber. | 12-22-2011 |
20110312687 | LOC DEVICE WITH LOW VOLUME HYBRIDIZATION CHAMBERS AND REAGENT RESERVOIRS FOR GENETIC ANALYSIS USING ELECTROCHEMILUMINESCENT TARGET DETECTION - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL), resonance energy transfer probes for hybridization with the target nucleic acid sequences, each of the probes containing a sequence complementary to the target nucleic acid sequence, and an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, hybridization chambers containing the probes for hybridization with the targets, at least one of the heaters and a pair of the electrodes, and, a reagent reservoir containing a reagent for addition to the fluid, wherein, the hybridization chambers each have a volume less than 900,000 cubic microns and the reagent reservoir has a volume less than 1,000,000,000 cubic microns. | 12-22-2011 |
20110312688 | MICROFLUIDIC DEVICE WITH PCR CHAMBER BETWEEN SUPPORTING SUBSTRATE AND HEATER - A microfluidic device having a supporting substrate, a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having a PCR chamber, and, a heater element for heating the nucleic acid sequences within the PCR chamber, wherein, the PCR chamber is between the heater element and the supporting substrate. | 12-22-2011 |
20110312689 | MICROFLUIDIC DEVICE WITH SENSOR-TRIGGERED PHOTODETECTION OF FLUORESCENT PROBE-TARGET HYBRID - A microfluidic device having a supporting substrate, an array of probes, each of the probes having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, CMOS circuitry on the substrate, the CMOS circuitry having an array of photodiodes for sensing deactivation of the excitation light and generating an output signal in response to the fluorescence signals from the probe-target hybrids within the array of probes. | 12-22-2011 |
20110312690 | MICROFLUIDIC DEVICE WITH PCR SECTION HAVING TWO-DIMENSIONAL CONTROL OF INPUT HEAT FLUX DENSITY - A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having a microchannel configured to have a plurality of mutually parallel, adjacent channel sections, and a plurality of elongate heaters positioned end to end along each of the channel sections, wherein, each of the heaters are independently operable for two-dimensional control of heat flux density to the PCR section. | 12-22-2011 |
20110312691 | LOC DEVICE WITH ELECTROCHEMILUMINESCENT PROBES INCLUDING POSITIVE AND NEGATIVE CONTROL PROBES - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, at least one positive control probe that has one of the ECL luminophores but not the functional moiety for quenching photon emission, at least one negative control probe that does not have one of the ECL luminophores, and, electrodes for receiving an electrical pulse to excite the ECL luminophores. | 12-22-2011 |
20110312692 | LOC DEVICE FOR PATHOGEN DETECTION WITH DIALYSIS, THERMAL LYSIS AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, the lysis section having a lysis chamber and a heater for lysing the pathogens while the sample is in the lysis chamber, and, a nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material, wherein, the dialysis section, the lysis section and the nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312693 | MICROFLUIDIC DEVICE WITH FEEDBACK CONTROLLED PCR SECTION - A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having at least one heater, and, at least one sensor, wherein, the at least one sensor is configured to provide output for feedback control of the at least one heater. | 12-22-2011 |
20110312694 | MICROFLUIDIC DEVICE WITH DELAY-TRIGGERED PHOTODETECTION OF FLUORESCENT PROBE-TARGET HYBRID - A microfluidic device having a supporting substrate, a probe having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, CMOS circuitry on the substrate, the CMOS circuitry having a photosensor for generating an output signal in response to the fluorescence signal, wherein, the CMOS circuitry is configured to initiate a time delay upon deactivation of the excitation light before activating the photosensor. | 12-22-2011 |
20110312695 | LOC DEVICE WITH HYBRIDIZATION CHAMBER ARRAY WITH POSITIVE CONTROL CHAMBER CONTAINING ELECTROCHEMILUMINESCENT REPORTER - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, hybridization chambers containing the probes for detection of the targets, and a pair of the electrodes, and, at least one positive control chamber containing positive control probes that have the ECL luminophore but not the functional moiety for quenching photon emission. | 12-22-2011 |
20110312696 | LOC DEVICE FOR PATHOGEN DETECTION WITH DIALYSIS, CHEMICAL LYSIS AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the cells in the lysis section, and, a nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material, wherein, the dialysis section, the lysis section and the nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312697 | MICROFLUIDIC DEVICE WITH TEMPERATURE FEEDBACK CONTROLLED PCR SECTION - A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having at least one heater, and, at least one temperature sensor, wherein, the at least one temperature sensor is configured to provide output for feedback control of the at least one heater. | 12-22-2011 |
20110312698 | MICROFLUIDIC DEVICE WITH PCR SECTION HAVING SHORT THERMAL CYCLE TIMES - A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having at least one heater for thermally cycling the nucleic acid sequences through a denaturation phase, an annealing phase and a primer extension phase, wherein during use, the PCR section has a thermal cycle time of less than 30 seconds. | 12-22-2011 |
20110312699 | MICROFLUIDIC DEVICE WITH ON-CHIP SEMICONDUCTOR CONTROLLED PCR SECTION - A microfluidic device having a supporting substrate, a sample inlet for receiving a sample of biological material having nucleic acid sequences, a microsystems technology (MST) layer having a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having at least one heater for thermally cycling the nucleic acid sequences through a denaturation phase, an annealing phase and a primer extension phase, and, CMOS circuitry between the MST layer and the supporting substrate, the CMOS circuitry being connected to the at least one heater for operative control of the at least one heater during the thermal cycling. | 12-22-2011 |
20110312700 | MICROFLUIDIC DEVICE WITH PWM CONTROLLED PCR HEATER - A microfluidic device having a supporting substrate, a sample inlet for receiving a sample of biological material having nucleic acid sequences, a microsystems technology (MST) layer having a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having at least one heater for thermally cycling the nucleic acid sequences through a denaturing phase, an annealing phase and a primer extension phase, and, CMOS circuitry between the MST layer and the supporting substrate, the CMOS circuitry configured to energize the at least one heater with a pulse width modulated (PWM) signal during the thermal cycling. | 12-22-2011 |
20110312701 | LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET NUCLEIC ACID SEQUENCES WITH CALIBRATED PHOTODETECTION OF PROBES IN HYBRIDIZATION ARRAY - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, detection photodiodes for exposure to the photons emitted by the ECL luminophores, and, at least one calibration photodiode for exposure to ambient light, wherein during use, a difference between the output from any of the detection photodiode and the output from the calibration photodiode is compared to a predetermined threshold difference such that output differences greater than the predetermined threshold indicate the target is present. | 12-22-2011 |
20110312702 | LOC DEVICE FOR GENETIC ANALYSIS WITH DIALYSIS, CHEMICAL LYSIS AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating cells larger than a predetermined threshold in the sample from smaller constituents, whereby the cells larger than a predetermined threshold include target cells containing genetic material for analysis, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the target cells to release the genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the target cells in the lysis section, and, a nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences from the genetic material, wherein, the dialysis section, the lysis section and the nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312703 | MICROFLUIDIC DEVICE FOR RAPID PCR AMPLIFICATION - A microfluidic device having a supporting substrate, a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having a PCR chamber, and, a heater element for heating the nucleic acid sequences within the PCR chamber, wherein during use, the PCR section generates sufficient amplicon for hybridization with an array of probes in less than 10 minutes. | 12-22-2011 |
20110312704 | MICROFLUIDIC DEVICE FOR PCR AMPLIFICATION USING LOW PCR MIXTURE VOLUME - A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section with a PCR microchannel for thermally cycling a PCR mixture of the sample, polymerase, dNTP's and buffer solution to amplify the nucleic acid sequences, the PCR microchannel defining a flow-path for the sample such that flow of the sample along the PCR microchannel is driven by capillary action, wherein, the PCR mixture has a volume less than 400 nanoliters. | 12-22-2011 |
20110312705 | TEST MODULE FOR PCR AMPLIFICATION USING LOW PCR MIXTURE VOLUME - A test module for amplifying nucleic acid sequences in a sample fluid, the test module having an outer casing with receptacle for receiving the sample fluid, a polymerase chain reaction (PCR) section with a PCR microchannel for thermally cycling a PCR mixture of the sample fluid, polymerase, dNTP's, primers, and buffer solution to amplify the nucleic acid sequences, wherein, the PCR mixture has a volume less than 400 nanoliters. | 12-22-2011 |
20110312706 | LOC DEVICE WITH HYBRIDIZATION CHAMBERS CONTAINING PROBES FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET NUCLEIC ACID SEQUENCES IN A FLUID AND CALIBRATION CHAMBER CONTAINING PROBES SEALED FROM THE FLUID - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having hybridization chambers containing electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the ECL probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, at least one calibration chamber containing probes sealed from contact with the fluid, and, electrodes for receiving an electrical pulse to excite the ECL luminophores. | 12-22-2011 |
20110312707 | GENETIC ANALYSIS LOC FOR PCR AMPLIFICATION OF NUCLEIC ACIDS - A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing buffer, dNTPs, primers, and polymerase for addition to the sample, and, a polymerase chain reaction (PCR) section for thermal cycling the sample, buffer, dNTPs, primers, and polymerase to amplify the target nucleic acid sequences. | 12-22-2011 |
20110312708 | GENETIC ANALYSIS LOC FOR ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS - A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing buffer, dNTPs, primers, and polymerase for addition to the sample to form an amplification mix, and, a nucleic acid amplification section for controlling the temperature of the amplification mix during isothermal amplification of the target nucleic acid sequences. | 12-22-2011 |
20110312709 | LOC DEVICE FOR DETECTING TARGET NUCLEIC ACID SEQUENCES USING ELECTROCHEMILUMINESCENT PROBES AND CALIBRATION PROBES WITH DETECTION PHOTOSENSORS AND CALIBRATION PHOTOSENSORS - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the ECL probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, at least one calibration source configured to generate a calibration emission, electrodes for receiving an electrical pulse to excite the ECL luminophores, at least one detection photodiode for sensing photon emissions from the ECL luminophores and generating a detection output, at least one calibration photodiode for sensing the calibration emission and generating a calibration output, and, CMOS circuitry having a differential circuit for subtracting the calibration output from the detection output. | 12-22-2011 |
20110312710 | LOC DEVICE FOR PATHOGEN DETECTION, GENETIC ANALYSIS AND PROTEOMIC ANALYSIS WITH DIALYSIS, CHEMICAL LYSIS, INCUBATION AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for pathogen detection, genetic analysis and proteomic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a leukocyte dialysis section for dividing the sample into a leukocyte stream, and a pathogen and erythrocyte stream containing cells and sample constituents smaller than a first size threshold, a pathogen dialysis section for dividing the pathogen and erythrocyte stream into an erythrocyte stream and a pathogen stream containing cells and sample constituents smaller than a second size threshold, a leukocyte lysis section downstream of the leukocyte dialysis section for lysing the leukocytes with a lysis reagent to release genetic material and proteins therein, a leukocyte incubation section downstream of the leukocyte lysis section for enzymatic reaction of the genetic material with enzymes, a leukocyte nucleic acid amplification section downstream of the leukocyte incubation section for amplifying nucleic acid sequences, a pathogen lysis section downstream of the pathogen dialysis section for lysing the pathogens with a lysis reagent to release genetic material and proteins therein, a pathogen incubation section downstream of the pathogen lysis section for enzymatic reaction of the genetic material with enzymes, a pathogen nucleic acid amplification section downstream of the pathogen incubation section for amplifying nucleic acid sequences, an erythrocyte lysis section downstream of the pathogen dialysis section for lysing the erythrocytes with a lysis reagent to release proteins therein, wherein, the leukocyte dialysis section, the pathogen dialysis section, the leukocyte lysis section, the pathogen lysis section, the erythrocyte lysis section, the leukocyte incubation section, the pathogen incubation section, the leukocyte nucleic acid amplification section and the pathogen nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312711 | MICROFLUIDIC DEVICE WITH CONTROLLABLE SHUNTS PERIPHERAL TO INTEGRATED PHOTODIODES - A microfluidic device having a probe for hybridization with a target nucleic acid sequence, a photodiode for detecting fluorescence emissions generated by hybridization of the probe in response to an excitation light, and, a shunt transistor between the photodiode anode and a voltage source, the shunt transistor being configured to remove carriers generated by absorption of photons of the excitation light in the photodiode, wherein, a peripheral edge of the photodiode and a peripheral edge of the shunt transistor contiguously abut each other. | 12-22-2011 |
20110312712 | GENETIC ANALYSIS LOC FOR PCR AMPLIFICATION OF NUCLEIC ACIDS FROM WHOLE BLOOD - A lab-on-a-chip (LOC) device for genetic analysis of a whole blood sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the whole blood sample, a plurality of reagent reservoirs containing buffer, dNTPs, primers, and polymerase for addition to the whole blood sample, and, a polymerase chain reaction (PCR) section for thermal cycling the whole blood sample, buffer, dNTPs, primers, and polymerase to amplify the target nucleic acid sequences. | 12-22-2011 |
20110312713 | MICROFLUIDIC TEST MODULE WITH LOW-VOLUME HYBRIDIZATION CHAMBERS FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET NUCLEIC ACID SEQUENCES IN A FLUID - A microfluidic test module for detecting target nucleic acid sequences in a fluid, the test module having an outer casing configured for hand-held portability, the outer casing having an inlet for receiving the fluid containing the target nucleic acid sequences, a hybridization chamber mounted in the casing, the hybridization chamber containing electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the ECL probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and electrodes for receiving an electrical pulse to excite the ECL luminophores, wherein, the hybridization chamber has a volume less than 900,000 cubic microns. | 12-22-2011 |
20110312714 | GENETIC ANALYSIS LOC FOR AMPLIFICATION OF NUCLEIC ACIDS USING DNA POLYMERASES OF THERMOPHILES - A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing buffer, dNTPs, primers, and polymerase derived from a thermophilic bacterium for addition to the sample to form an amplification mixture, and, a nucleic acid amplification section for controlling the temperature of the amplification mixture during amplification of the target nucleic acid sequences. | 12-22-2011 |
20110312715 | MICROFLUIDIC TEST MODULE WITH LOW MASS ELECTROCHEMILUMINESCENT PROBE SPOTS - A microfluidic test module for detecting target nucleic acid sequences in a fluid, the test module having an outer casing having an inlet for receiving the fluid containing the target nucleic acid sequences, electrode pairs for receiving an electrical pulse, electrochemiluminescent (ECL) probe spots in contact with each of the electrode pairs respectively, the ECL probe spots containing ECL probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, such that the electrical pulse to the electrode pair excites the ECL luminophores, wherein, the mass of the ECL probes in each of the probe spots is less than 270 picograms. | 12-22-2011 |
20110312716 | GENETIC ANALYSIS LOC FOR NUCLEIC ACID AMPLIFICATION USING AN ISOTHERMAL REACTION - A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing buffer solution, dNTPs, primers, and polymerase for addition to the sample to form an amplification mixture, and, a nucleic acid amplification section for maintaining the amplification mixture at a predetermined temperature during isothermal amplification of the target nucleic acid sequences. | 12-22-2011 |
20110312717 | LOC DEVICE FOR PATHOGEN DETECTION WITH DIALYSIS, INCUBATION AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a plurality of reagent reservoirs, a pathogen dialysis section for separating pathogens from constituents smaller than a predetermined threshold, a pathogen lysis section downstream of the pathogen dialysis section for lysing the pathogens with a lysis reagent to release genetic material therein, an incubation section downstream of the pathogen dialysis section, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, and, a nucleic acid amplification section downstream of the incubation section for amplifying nucleic acid sequences from the genetic material, wherein, the pathogen dialysis section, the pathogen lysis section, the incubation section and the nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312718 | GENETIC ANALYSIS LOC FOR NUCLEIC ACID AMPLIFICATION USING RECOMBINASE POLYMERASE AMPLIFICATION - A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing dNTPs, primers, recombinase, DNA polymerase and buffer solution for addition to the sample to form an amplification mixture, and, a nucleic acid amplification section for maintaining the amplification mixture at a predetermined temperature during recombinase polymerase amplification (RPA) of the target nucleic acid sequences. | 12-22-2011 |
20110312719 | TEST MODULE WITH INTEGRAL PHOTOSENSOR FOR ELECTROCHEMILUMINESCENT DETECTION OF HYBRIDIZATION - A test module for detecting target nucleic acid sequences in a fluid, the test module having an outer casing having a receptacle for receiving the fluid containing the target nucleic acid sequences, electrochemiluminescent (ECL) probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, and, a detection photosensor for exposure to the photons emitted by the ECL luminophores. | 12-22-2011 |
20110312720 | LOC DEVICE FOR GENETIC ANALYSIS WITH DIALYSIS AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating smaller constituents from larger constituents, the smaller constituents being smaller than a predetermined size threshold, and the larger constituents being larger than the predetermined size threshold, and, a nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material, wherein, the dialysis section and the nucleic acid amplification section are both supported on the supporting substrate. | 12-22-2011 |
20110312721 | LOC DEVICE FOR GENETIC ANALYSIS WITH DIALYSIS, INCUBATION, AND NUCLEIC ACID AMPLIFICATION - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a plurality of reagent reservoirs, a dialysis section for separating cells larger than a predetermined threshold in the sample from smaller constituents, whereby the cells larger than a predetermined threshold include target cells containing genetic material for analysis, an incubation section downstream of the dialysis section, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, and, a nucleic acid amplification section downstream of the incubation section for amplifying nucleic acid sequences from the genetic material, wherein, the dialysis section, the incubation section and the nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312722 | PORTABLE TEST MODULE FOR EXCITATION OF ELECTROCHEMILUMINESCENT PROBES - A portable test module for excitation of electrochemiluminescent probes configured to detect target nucleic acid sequences, the test module having an outer casing configured for hand-held portability, the outer casing having a receptacle for receiving a fluid containing the target nucleic acid sequences, electrochemiluminescent (ECL) probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and, electrodes for receiving an electrical pulse to excite the ECL luminophores, wherein during use, the ECL probes that have detected one of the target nucleic acid sequences reconfigure such that the functional moiety does not quench the photon emission from the ECL luminophore when excited by the electrodes. | 12-22-2011 |
20110312723 | GENETIC ANALYSIS LOC FOR NUCLEIC ACID AMPLIFICATION USING NUCLEIC ACID SEQUENCE BASED AMPLIFICATION - A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing dNTPs, primers, reverse transcriptase, RNA polymerase, and buffer solution for addition to the sample to form an amplification mixture, and, a nucleic acid amplification section for maintaining the amplification mixture at a predetermined temperature during isothermal amplification of the target nucleic acid sequences. | 12-22-2011 |
20110312724 | LOC DEVICE FOR PATHOGEN DETECTION AND GENETIC ANALYSIS WITH INCUBATION, NUCLEIC ACID AMPLIFICATION AND PREHYBRIDIZATION FILTERING - A lab-on-a-chip (LOC) device for pathogen detection and genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample containing genetic material, a supporting substrate, a plurality of reagent reservoirs, an incubation section downstream of the inlet, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, and, a nucleic acid amplification section downstream of the incubation section for amplifying nucleic acid sequences in the genetic material, and, a dialysis section downstream of the nucleic acid amplification section for prehybridization filtration of amplicon produced by the nucleic acid amplification section, the dialysis section being configured to remove cell debris from the amplicon, wherein, the incubation section, the nucleic acid amplification section and the dialysis section are all supported on the supporting substrate. | 12-22-2011 |
20110312725 | USB-INTERFACEABLE PORTABLE TEST MODULE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGETS - A test module for excitation of electrochemiluminescent probes configured to detect target nucleic acid sequences, the test module having an outer casing having a receptacle for receiving a fluid containing the target nucleic acid sequences, electrochemiluminescent (ECL) probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, a detection photosensor for exposure to the photons emitted by the ECL luminophores, control circuitry providing the electrical pulse to the electrodes, and, a universal serial bus (USB) connection such that the outer casing is configured as a USB drive for transmitting data regarding detection of the targets in the fluid to an external device, wherein during use, the ECL probes that have detected one of the target nucleic acid sequences reconfigure such that the functional moiety does not quench the photon emission from the ECL luminophore when excited by the electrodes. | 12-22-2011 |
20110312726 | MICROFLUIDIC DEVICE WITH CONTROLLABLE SHUNTS INSIDE INTEGRATED PHOTODIODES - A microfluidic device having a probe for hybridization with a target nucleic acid sequence to form a probe-target hybrid, a photodiode having an active area for detecting fluorescence emissions generated by the probe-target hybrid in response to an excitation light, and, a shunt transistor between the photodiode anode and a voltage source, the shunt transistor being configured to remove carriers generated by absorption of photons of the excitation light in the photodiode, wherein, the shunt transistor is encompassed by the active area. | 12-22-2011 |
20110312727 | LOC DEVICE WITH PARALLEL NUCLEIC ACID AMPLIFICATION FUNCTIONALITY - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample containing genetic material, a supporting substrate, a plurality of reagent reservoirs, a first nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material in parallel with the first nucleic acid amplification section, wherein, the first nucleic acid amplification section and the second nucleic acid amplification section are both supported on the supporting substrate. | 12-22-2011 |
20110312728 | MICROFLUIDIC DEVICE WITH NON-IMAGING OPTICS - A microfluidic device having a supporting substrate, a hybridization chamber containing probes having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form probe-target hybrids, the probe-target hybrids being configured to generate a fluorescence signal in response to an excitation light, and, a photodiode with an active area and an optical axis extending normal to the active area and through the hybridization chamber, wherein, the active area is less than 249 microns from the probe-target hybrids. | 12-22-2011 |
20110312729 | TEST MODULE USING TRANSITION METAL-LIGAND COMPLEX, ELECTROCHEMILUMINESCENT LUMINOPHORES - A test module for excitation of electrochemiluminescent probes configured to detect target nucleic acid sequences, the test module having an outer casing with an inlet for receiving a fluid containing the target nucleic acid sequences, electrochemiluminescent (ECL) probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and, electrodes for receiving an electrical pulse to excite the ECL luminophores, such that the ECL probes that have detected one of the target nucleic acid sequences reconfigure such that the functional moiety does not quench the photon emission from the ECL luminophore when excited by the electrodes, wherein, the ECL luminophore has a transition metal-ligand complex. | 12-22-2011 |
20110312730 | LOC DEVICE WITH PARALLEL DNA AND RNA AMPLIFICATION FUNCTIONALITY - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample containing genetic material including DNA and RNA, a supporting substrate, a plurality of reagent reservoirs, a first nucleic acid amplification section for amplifying at least some of the genetic material, and, a second nucleic acid amplification section for amplifying at least some the genetic material in parallel with the first nucleic acid amplification section, wherein, the first nucleic acid amplification section and the second nucleic acid amplification section are both supported on the supporting substrate. | 12-22-2011 |
20110312731 | MICROFLUIDIC DEVICE WITH LARGE ANGLE OF COLLECTION OF EMISSION LIGHT - A microfluidic device having a supporting substrate, a hybridization chamber containing probes having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form probe-target hybrids and generate a fluorescence signal in response to an excitation light, and, a photodiode with an active area and an optical axis extending normal to the active area and through the hybridization chamber, wherein, the hybridization chamber has a floor surface positioned parallel to the active area of the photodiode, the floor surface having a centroid and the active area being encompassed within a cone having the centroid at its vertex, and a vertex angle less than 173°. | 12-22-2011 |
20110312732 | TEST MODULE USING LANTHANIDE METAL-LIGAND COMPLEX, ELECTROCHEMILUMINESCENT LUMINOPHORES - A test module for excitation of electrochemiluminescent probes configured to detect target nucleic acid sequences, the test module having an outer casing with an inlet for receiving a fluid containing the target nucleic acid sequences, electrochemiluminescent (ECL) probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and, electrodes for receiving an electrical pulse to excite the ECL luminophores, such that the ECL probes that have detected one of the target nucleic acid sequences reconfigure such that the functional moiety does not quench the photon emission from the ECL luminophore when excited by the electrodes, wherein, the ECL luminophore has a lanthanide metal-ligand complex. | 12-22-2011 |
20110312733 | LOC DEVICE WITH NUCLEIC ACID AMPLIFICATION SECTION - A lab-on-a-chip (LOC) device for amplifying nucleic acid sequences, the LOC device having an inlet for receiving a sample containing genetic material, a supporting substrate, a plurality of reagent reservoirs, and, a nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material, wherein, the nucleic acid amplification section is supported on the supporting substrate. | 12-22-2011 |
20110312734 | TEST MODULE WITH SUSPENDED ELECTROCHEMILUMINESCENT PROBES - A test module for detecting target nucleic acid sequences in a fluid, the test module having an outer casing having an inlet for receiving the fluid containing the target nucleic acid sequences, electrode pairs for receiving an electrical pulse, electrochemiluminescent (ECL) probes adjacent each of the electrode pairs respectively, each of the ECL probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, such that the electrical pulse to the electrode pair excites the ECL luminophores, wherein, the ECL probes are not anchored to a surface and form a suspension in the fluid containing the target nucleic acid sequences during detection. | 12-22-2011 |
20110312735 | MICROFLUIDIC DEVICE WITH NUCLEIC ACID AMPLIFICATION SECTION - A microfluidic device for amplifying nucleic acid sequences, the microfluidic device having an inlet for receiving a sample containing genetic material, a plurality of reagent reservoirs containing reagents for addition to the sample, and, a nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material. | 12-22-2011 |
20110312736 | MICROFLUIDIC DEVICE WITH FLOW RATE SENSOR - A microfluidic device for processing a fluid, the microfluidic device having an inlet for receiving the fluid, functional sections for processing the fluid, a flow-path extending from the inlet into at least one of the functional sections, a flow rate sensor with a conductive element positioned in proximity of the fluid flowing along the flow-path, CMOS circuitry configured for measuring the temperature of the conductive element, wherein, the CMOS circuitry is configured to provide a predetermined current through the conductive element and measure the electrical resistance of the conductive element such that a flow speed is derived from the current, the temperature sensor output and the electrical resistance, and a flow rate is derived using the flow speed and the flow-path cross section transverse to the flow direction at the conductive element. | 12-22-2011 |
20110312737 | SINGLE-USE TEST MODULE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGETS - A test module for detecting target nucleic acid sequences in a fluid, the test module having an outer casing with an inlet for receiving a fluid containing the target nucleic acid sequences, an array of electrochemiluminescent (ECL) probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, and, electrodes positioned for receiving an electrical pulse, the probe-target hybrids being configured to emit a photon of light when excited by current between the electrodes, and, a photosensor for detecting the light emitted by the probes, wherein during use, addition of the fluid to the probes prevents subsequent addition of other fluid to the probes. | 12-22-2011 |
20110312738 | MICROFLUIDIC DEVICE WITH LIQUID SENSOR - A microfluidic device for processing a fluid, the microfluidic device having an inlet for receiving the fluid, functional sections for processing the fluid, a flow-path extending from the inlet into at least some of the functional sections, circuitry for operative control of the functional sections, and, a liquid sensor with electrodes positioned for contact with the fluid flowing along the flow-path, wherein, the circuitry is configured to provide a voltage across the electrodes such that current above a predetermined threshold is indicative of liquid at the electrodes. | 12-22-2011 |
20110312739 | SINGLE-USE TEST MODULE FOR PCR AMPLIFICATION OF TARGETS AND ELECTROCHEMILUMINESCENT DETECTION OF TARGETS - A test module for amplifying and detecting target nucleic acid sequences in a fluid, the test module having an outer casing with a receptacle for receiving a fluid containing the target nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the target nucleic acid sequences, an array of electrochemiluminescent (ECL) probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, and, electrodes positioned for receiving an electrical pulse, the probe-target hybrids being configured to emit a photon of light when excited by current between the electrodes, and, a photosensor for detecting the light emitted by the probes, wherein during use, addition of the fluid to the probes prevents subsequent addition of other fluid to the probes. | 12-22-2011 |
20110312740 | MICROFLUIDIC DEVICE WITH CAPILLARY MENISCUS MARCHING VELOCITY SENSOR - A microfluidic device for processing a fluid, the microfluidic device having an inlet for receiving the fluid, functional sections for processing the fluid, a flow-path extending from the inlet into at least some of the functional sections, circuitry for operative control of the functional sections, and, a capillary meniscus marching velocity sensor having a plurality of liquid sensors spaced along the flow-path, each of the liquid sensors having electrodes positioned for contact with the fluid flowing along the flow-path, wherein, the circuitry is configured to provide a voltage across the electrodes such that current above a predetermined threshold is indicative of liquid at the electrodes and used to derive a velocity of the liquid flow front. | 12-22-2011 |
20110312741 | MICROFLUIDIC DEVICE FOR ANALYSIS OF MITOCHONDRIAL DNA - A microfluidic device for analysis of mitochondrial DNA in a sample, the microfluidic device having an inlet for receiving a sample of biological material having cells with an outer cellular membrane containing mitochondria and a nucleus, the mitochondria having a mitochondrial membrane containing mitochondrial DNA and the nuclei having a nuclear membrane containing nuclear DNA, a lysis section for lysing the outer cellular membrane and the mitochondrial membrane while the nuclear membrane remains intact, and, probes for hybridization with target nucleic acid sequences in the mitochondrial DNA to form probe-target hybrids. | 12-22-2011 |
20110312742 | SINGLE-USE MICROFLUIDIC DEVICE - A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing a target nucleic acid sequence, a probe with a nucleic acid sequence for hybridization with the target nucleic acid sequence to form a probe-target hybrid and generate a fluorescence signal in response to an excitation light, and, a photosensor for sensing the fluorescence signal, wherein during use, addition of the biological sample to the inlet prevents fluid communication between a fluid subsequently added to the inlet and the probe. | 12-22-2011 |
20110312743 | LOC DEVICE FOR DETECTION OF TARGET NUCLEIC ACID SEQUENCES USING ELECTRODES CONFIGURED FOR ELECTROCHEMILUMINESCENCE OF LUMINOPHORES WITHOUT A COREACTANT - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and, electrodes for receiving an electrical pulse to excite the ECL luminophores, wherein, the electrodes are arranged into pairs, each having an anode and a cathode, the anode and the cathode being separated by a dielectric gap between 0.4 microns and 2 microns wide. | 12-22-2011 |
20110312744 | MICROFLUIDIC DEVICE FOR AMPLIFYING MITOCHONDRIAL DNA IN A BIOLOGICAL SAMPLE - A microfluidic device for amplifying mitochondrial DNA in a sample, the microfluidic device having an inlet for receiving a sample of biological material having cells with an outer cellular membrane containing mitochondria and a nucleus, the mitochondria having a mitochondrial membrane containing mitochondrial DNA and the nuclei having a nuclear membrane containing nuclear DNA, a lysis section for lysing the outer cellular membrane and the mitochondrial membrane while the nuclear membrane remains intact, and, a nucleic acid amplification section configured for amplifying target nucleic acid sequences in the mitochondrial DNA. | 12-22-2011 |
20110312745 | MICROFLUIDIC TEST MODULE WITH PHOTOSENSOR - A test module for analyzing a sample fluid containing target molecules, the test module having an outer casing with a receptacle for receiving the sample fluid, probes for reaction with the target molecules to form probe-target complexes, the probe-target complexes being configured to emit photons when excited, and, a photosensor for detecting photons emitted by the probe-target complexes. | 12-22-2011 |
20110312746 | MICROFLUIDIC DEVICE WITH CHEMICAL LYSIS SECTION - A microfluidic device for lysing cells in a fluid, the microfluidic device having an inlet for receiving a fluid containing cells, a lysis reagent reservoir containing a lysis reagent and having an outlet valve, and, a lysis section in fluid communication with the inlet for holding the fluid during lysis of the cells, wherein, the outlet valve is upstream of the lysis section and configured to open as the fluid flows into the lysis section thereby adding the lysis reagent to the fluid. | 12-22-2011 |
20110312748 | LOC WITH INTEGRAL PHOTOSENSOR FOR DETECTION OF HYBRIDIZATION ASSAY RESULTS - A microfluidic device having a supporting substrate, an array of probes for hybridization with a target nucleic acid sequence to form a probe-target hybrid and generate a fluorescence signal in response to an excitation light, and, a photosensor positioned adjacent the array of probes, wherein, the photosensor is configured for sensing which probes within the array of probes generated the fluorescence signal. | 12-22-2011 |
20110312749 | MICROFLUIDIC DEVICE WITH THERMAL LYSIS SECTION - A microfluidic device for lysing cells in a fluid, the microfluidic device having an inlet channel for receiving a fluid containing cells, a lysis section in fluid communication with the inlet channel for holding the fluid during lysis of the cells, and, a lysis heater for heating the fluid in the lysis section such that the cells lyse, wherein, the inlet channel is configured for filling the lysis section by capillary action. | 12-22-2011 |
20110312750 | MICROFLUIDIC DEVICE WITH TOTAL REAGENT STORAGE - A microfluidic device for biochemical processing and analysis, the microfluidic device having a supporting substrate, a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving biochemical liquid, and, a plurality of reagent reservoirs containing all reagents necessary for the biochemical processing, wherein, each of the reagent reservoirs have an outlet with a surface tension valve for retaining the reagent in the reservoir with a reagent meniscus until contact with the biochemical liquid removes the reagent meniscus. | 12-22-2011 |
20110312751 | MICROFLUIDIC DEVICE FOR DETECTION OF MITOCHONDRIAL DNA VIA FLUORESCENCE MODULATED BY HYBRIDIZATION - A microfluidic device for analysis of mitochondrial DNA in a sample, the microfluidic device having an inlet for receiving a sample of biological material having cells with mitochondria containing mitochondrial DNA, a lysis section for lysing the mitochondria to release the mitochondrial DNA, fluorescence resonance energy transfer (FRET) probes for hybridization with target nucleic acid sequences in the mitochondrial DNA to form probe-target hybrids, and, a photosensor for detecting the probe-target hybrids. | 12-22-2011 |
20110312752 | MICROFLUIDIC DEVICE WITH LOW-VOLUME REAGENT RESERVOIR - A microfluidic device for biochemical processing and analysis, the microfluidic device having a supporting substrate, a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving biochemical liquid, and, a reagent reservoir containing a reagent necessary for the biochemical processing, wherein, the reagent reservoir has a volume less than 1000,000,000 cubic microns. | 12-22-2011 |
20110312753 | LOC WITH INTEGRAL LED DRIVER FOR EXCITATION LED - A microfluidic device having a supporting substrate, a probe with a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid and generate a fluorescence signal in response to an excitation light, and, CMOS circuitry on the supporting substrate for operative control of the excitation light. | 12-22-2011 |
20110312754 | MICROFLUIDIC DEVICE FOR DETECTION OF MITOCHONDRIAL DNA VIA ELECTROCHEMILUMINESCENCE MODULATED HYBRIDIZATION - A microfluidic device for analysis of mitochondrial DNA in a sample, the microfluidic device having an inlet for receiving a sample of biological material having cells with mitochondria containing mitochondrial DNA, a lysis section for lysing the mitochondria to release the mitochondrial DNA, electrochemiluminescent (ECL) resonance energy transfer probes for hybridization with target nucleic acid sequences in the mitochondrial DNA to form probe-target hybrids, and, a photosensor for detecting the probe-target hybrids. | 12-22-2011 |
20110312755 | TEST MODULE WITH CHEMICAL LYSIS SECTION - A test module for lysing cells in a fluid, the test module having an outer casing with receptacle for receiving a fluid containing cells, a lysis reagent reservoir containing a lysis reagent and having an outlet valve, and, a lysis section in fluid communication with the receptacle for holding the fluid during lysis of the cells, wherein, the outlet valve is upstream of the lysis section and configured to open as the fluid flows into the lysis section thereby adding the lysis reagent to the fluid. | 12-22-2011 |
20110312756 | MICROFLUIDIC DEVICE WITH LOW REAGENT VOLUMES - A microfluidic device for biochemical processing and analysis, the microfluidic device having a supporting substrate, a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving biochemical liquid, and, a reagent reservoir containing a reagent necessary for the biochemical processing, wherein, the reagent reservoir contains less than 1,000,000,000 cubic microns of reagent. | 12-22-2011 |
20110312757 | REAGENT MICROVIAL WITH DIGITAL MEMORY - A reagent microvial for a reagent dispensing apparatus, the microvial having a container for holding a volume of reagent, a digital memory for data relating to the reagent, a droplet generator for ejecting droplets of the reagent from the container, and, electrical contacts for connection to a control processor in the reagent dispensing apparatus to receive drive pulses for the droplet generator and provide the data to the control processor. | 12-22-2011 |
20110312758 | TEST MODULE WITH THERMAL LYSIS SECTION - A test module for lysing cells in a fluid, the test module having an outer casing with receptacle for receiving a sample that is a fluid containing cells, a lysis section in fluid communication with the receptacle for holding the fluid during lysis of the cells, and, a lysis heater for heating the fluid in the lysis section to lyse cells, wherein, the test module is configured for drawing the fluid from the receptacle to the lysis section by capillary action. | 12-22-2011 |
20110312759 | GENETIC ANALYSIS LOC WITH REAGENT RESERVOIR - A LOC device for performing a genetic diagnostic assay, the LOC device having a supporting substrate, a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving a biological sample containing target nucleic acid sequences, probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, a flow-path extending from the inlet to the probes, and, a reagent reservoir containing a reagent for addition to the sample in the flow-path upstream of the probes. | 12-22-2011 |
20110312760 | REAGENT MICROVIAL WITH AUTHENTICATION INTEGRATED CIRCUIT - A reagent microvial for a reagent dispensing apparatus, the microvial having a container for holding a volume of reagent, an integrated circuit with digital memory for data relating to an identity of the microvial, and, electrical contacts for connection to a control processor in the reagent dispensing apparatus to provide the data to the control processor for comparison with a list of authentic microvial identities. | 12-22-2011 |
20110312761 | TEST MODULE FOR CHEMICALLY AND THERMALLY LYSING CELLS - A test module for lysing cells in a fluid, the test module having an outer casing with receptacle for receiving a fluid containing cells, a lysis reagent reservoir containing a lysis reagent and having a valve, and, a lysis section in fluid communication with the receptacle for holding the fluid during lysis of the cells, the lysis section having a lysis heater for heating the fluid in the lysis section, wherein, the valve is upstream of the lysis section and configured to open as the fluid flows into the lysis section thereby adding the lysis reagent to the fluid. | 12-22-2011 |
20110312762 | LOC FOR DETECTION OF HYBRIDIZATION OF NUCLEIC ACID SEQUENCES WITH FLUORESCENCE RESONANCE ENERGY TRANSFER (FRET) PROBES - A lab-on-a-chip (LOC) device having a supporting substrate, a microsystems technologies (MST) layer with a hybridization section that has an array of fluorescence resonance energy transfer (FRET) probes for hybridization with target nucleic acid sequences in a fluid, and, a photosensor for detecting hybridization of probes within the array of probes. | 12-22-2011 |
20110312763 | GENETIC ANALYSIS LOC WITH IN-LOC STORAGE OF ALL REQUIRED REAGENTS - A lab-on-a-chip (LOC) device for genetic analysis having a supporting substrate, a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving a biological sample containing target nucleic acid sequences, probes for hybridization with the target nucleic acid sequence to form probe-target hybrids, and, a plurality of reagent reservoirs containing all reagents necessary for performing the diagnostic assay. | 12-22-2011 |
20110312764 | MICROFLUIDIC DEVICE WITH INCUBATOR - A microfluidic device having a supporting substrate, an inlet for receiving a fluid, a microsystems technology (MST) layer for processing the fluid, the MST layer incorporating an incubation section, the incubation section having at least one heater for maintaining the fluid at a predetermined incubation temperature, and, CMOS circuitry between the MST layer and the supporting substrate, the CMOS circuitry being connected to the at least one heater for operative control of the at least one heater. | 12-22-2011 |
20110312765 | GENETIC ANALYSIS LOC WITH LOW OLIGONUCLEOTIDE PROBE MASS AND LOW REAGENT VOLUME - A lab-on-a-chip (LOC) device for genetic analysis having a supporting substrate, a microsystems technology (MST) layer supported on the substrate, the MST layer having an inlet for receiving a biological sample containing target nucleic acid sequences, a hybridization section comprising an array of chambers with probes for hybridization with the target nucleic acid sequences to form probe-target hybrids, and, a reagent reservoir containing a reagent necessary for processing the biological sample prior to hybridization, wherein, the hybridization section contains less than 270 picograms of each probe per chamber and the reagent reservoir containing less than 1000,000,000 cubic microns of the reagent. | 12-22-2011 |
20110312766 | MICROFLUIDIC DEVICE WITH FEEDBACK CONTROLLED INCUBATION SECTION - A microfluidic device having a sample inlet for receiving a fluid sample, an incubation section for maintaining the fluid sample at an incubation temperature, the incubation section having at least one heater, and, at least one sensor, wherein, the at least one sensor is configured to provide output for feedback control of the at least one heater. | 12-22-2011 |
20110312767 | MICROFLUIDIC DEVICE WITH INCUBATION SECTION HAVING TEMPERATURE FEEDBACK - A microfluidic device having a sample inlet for receiving a fluid sample, an incubation section for maintaining the fluid sample at an incubation temperature, the incubation section having at least one heater and at least one temperature sensor, wherein, the at least one temperature sensor is configured to provide output for feedback control of the at least one heater. | 12-22-2011 |
20110312768 | LOC FOR DETECTION OF HYBRIDIZATION OF NUCLEIC ACID SEQUENCES WITH PCR AMPLIFICATION USING PRIMERS COVALENTLY ATTACHED TO STEM-AND-LOOP PROBES - A LOC device having a supporting substrate, primer-linked, stem-and-loop probes, each with a nucleic acid sequence that matches one of a plurality of target nucleic acid sequences, and a primer for elongating against the target nucleic acid sequence to form a complementary sequence such that during use the nucleic acid sequence matching the target nucleic acid sequence anneals to the complementary sequence to change a fluorescence emission from the probe in response to an excitation light, a polymerase chain reaction (PCR) section for amplifying the target nucleic acid sequences in the fluid prior to hybridization with the FRET probes, and, CMOS circuitry on the supporting substrate, the CMOS circuitry having operative control of the excitation light. | 12-22-2011 |
20110312769 | TEST MODULE WITH LOW-VOLUME REAGENT RESERVOIR - A test module for biochemical processing and analysis, the test module having an outer casing dimensioned for hand-held portability, the outer casing having a receptacle for receiving biochemical liquid, and, a reagent reservoir containing a reagent for addition to the biochemical liquid for use in chemical analysis of the biochemical liquid, wherein, the reagent reservoir has a volume less than 1000,000,000 cubic microns. | 12-22-2011 |
20110312770 | LOC FOR DETECTION OF HYBRIDIZATION OF NUCLEIC ACID SEQUENCES WITH NUCLEIC ACID AMPLIFICATION USING PRIMERS COVALENTLY ATTACHED TO LINEAR PROBES - A LOC device having a supporting substrate, a primer-linked, linear probe with a nucleic acid sequence that matches a target nucleic acid sequence, and a primer for elongating against the target nucleic acid sequence to form a complementary sequence such that during use the nucleic acid sequence matching the target nucleic acid sequence anneals to the complementary sequence to change a fluorescence emission from the probe in response to an excitation light, and, CMOS circuitry on the supporting substrate, the CMOS circuitry having operative control of the excitation light. | 12-22-2011 |
20110312771 | MICROFLUIDIC DEVICE WITH PWM CONTROLLED INCUBATION SECTION - A microfluidic device having a sample inlet for receiving a fluid sample, an incubation section in fluid communication with the sample inlet, the incubation section having at least one heater for maintaining the fluid sample at an incubation temperature for an incubation period, and, CMOS circuitry to energize the at least one heater with a pulse width modulated (PWM) signal. | 12-22-2011 |
20110312772 | LOC FOR DETECTION OF HYBRIDIZATION OF NUCLEIC ACID SEQUENCES WITH PCR AMPLIFICATION USING LINKER PRIMERS - A LOC device for amplifying nucleic acid sequences and detecting target nucleic acid sequences, the LOC device having a supporting substrate, restriction enzymes for digesting double stranded nucleic acid sequences at known restriction sites, linker primers for ligation to the restricted ends of the double stranded nucleic acid sequences, heaters for thermally cycling the nucleic acid sequences through a polymerase chain reaction (PCR) process, deoxyribonucleoside triphosphates (dNTPs) for extending the linker primers along the nucleic acid sequences, and, probes for hybridizing with target nucleic acid sequences. | 12-22-2011 |
20110312773 | MICROFLUIDIC DEVICE WITH FAULT-TOLERANT MULTIPLE VALVE ASSEMBLY - A microfluidic device having an inlet for receiving a liquid flowing through the microfluidic device, an outlet downstream of the inlet, and, a fault-tolerant multiple valve assembly having a plurality of flow-paths extending from the inlet to the outlet and a plurality of valves positioned along each of the flow-paths respectively. | 12-22-2011 |
20110312774 | MICROFLUIDIC DEVICE FOR DIFFUSIVE MIXING IN SMALL CROSS SECTIONAL AREA MICROCHANNEL - A microfluidic device having a supporting substrate, a microsystems technologies (MST) layer on the supporting substrate, the MST layer being configured to combine at least two liquids to form a flow of combined liquids, the MST layer having a diffusion mixing section for diffusive mixing of the at least two liquids, the mixing section having a microchannel defining a flow-path for the combined liquids, wherein, the channel cross section transverse to the flow direction is less than 100,000 square microns. | 12-22-2011 |
20110312775 | MICROFLUIDIC DEVICE WITH DIGITAL MEMORY - A microfluidic device for analyzing a sample fluid, the microfluidic device having a sample inlet for receiving the sample, a microsystems technology (MST) layer with functional sections for processing and analyzing the sample, and, CMOS circuitry with digital memory for storing data and operational information to operatively control the functional sections during processing and analysis of the sample. | 12-22-2011 |
20110312776 | GENETIC ANALYSIS LOC WITH HYBRIDIZATION ARRAY WITH POSITIVE CONTROL CHAMBERS INCORPORATING REPORTERS - A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing a target nucleic acid sequence, probes that each have a nucleic acid sequence for hybridization with the target nucleic acid sequence to form a probe-target hybrid, a fluorophore and a quencher configured such that the fluorophore emits a fluorescence signal in response to an excitation light and the quencher quenches the fluorescence signal when the probe is not hybridized, but fails to quench the fluorescence signal from the probe-target hybrid, and, a reporter with a fluorophore positioned for exposure to the excitation light simultaneously with the probes, wherein, the reporter always emits the fluorescence signal in response to the excitation light. | 12-22-2011 |
20110312777 | TEST MODULE WITH DIGITAL MEMORY - A test module for analyzing a sample fluid, the test module having a receptacle for receiving the sample, functional sections for processing and analyzing the sample, and, digital memory for storing data and operational information to operatively control the functional sections during processing and analysis of the sample. | 12-22-2011 |
20110312778 | GENETIC ANALYSIS LOC WITH HYBRIDIZATION ARRAY WITH NEGATIVE CONTROL CHAMBERS INCORPORATING PROBES DESIGNED TO BE NONCOMPLEMENTARY TO NUCLEIC ACID SEQUENCES IN THE AMPLICON - A lab-on-a-chip (LOC) device for genetic analysis having a supporting substrate, an inlet for receiving a biological sample containing target nucleic acid sequences, an array of probes each having a nucleic acid sequence for hybridization with the target nucleic acid sequences to form probe-target hybrids, and, a control probe designed to be complementary to sequences known to be absent from the biological sample. | 12-22-2011 |
20110312779 | OLIGONUCLEOTIDE SPOTTING DEVICE FOR OPERATION UNDER EXTERNAL MICROPROCESSOR CONTROL - An oligonucleotide spotting device for contactless spotting of probes onto a surface, the probes having nucleic acid sequences that are complementary to target nucleic acid sequences to be identified in a biological sample, the oligonucleotide spotting device having a supporting substrate, an array of ejectors, each having an actuator for ejecting droplets of liquid containing the oligonucleotide probes, CMOS circuitry for providing each of the drop ejection actuators with a drive pulse for droplet ejection, and, bond-pads for electrically connecting the CMOS circuitry and an external microprocessor controller for operative control of the array of ejectors. | 12-22-2011 |
20110312780 | GENETIC ANALYSIS LOC WITH HYBRIDIZATION ARRAY WITH NEGATIVE CONTROL CHAMBERS INCORPORATING PROBES WITH NO REPORTERS - A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing target nucleic acid sequences, probes that each have a nucleic acid sequence for hybridization with the target nucleic acid sequences to form probe-target hybrids, a fluorophore and a quencher configured such that the fluorophore emits a fluorescence signal in response to an excitation light and the quencher quenches the fluorescence signal when the probe is not hybridized, but fails to quench the fluorescence signal from the probe-target hybrid, and, a control probe with no fluorophore such that the control probe never emits the fluorescence signal in response to the excitation light. | 12-22-2011 |
20110312781 | LOC WITH DIGITAL MEMORY TO STORE GENETIC DATA UPDATES - A lab-on-a-chip (LOC) device for analyzing a sample fluid, the LOC device having a sample inlet for receiving the sample, a microsystems technology (MST) layer with functional sections for processing and analyzing the sample, and, CMOS circuitry with digital memory for storing genetic data, and configured to download genetic data updates from an external source. | 12-22-2011 |
20110312782 | OLIGONUCLEOTIDE SPOTTING DEVICE WITH DIGITAL MEMORY - An oligonucleotide spotting device for contactless spotting of probes onto a surface, the probes having nucleic acid sequences that are complementary to target nucleic acid sequences to be identified in a biological sample, the oligonucleotide spotting device having a supporting substrate, an array of ejectors, each having an actuator for ejecting droplets of liquid containing the oligonucleotide probes, and, CMOS circuitry for providing each of the drop ejection actuators with a drive pulse for droplet ejection, wherein, the CMOS circuitry has a digital memory for storing data related to the device. | 12-22-2011 |
20110312783 | GENETIC ANALYSIS LOC WITH HYBRIDIZATION ARRAY WITH NEGATIVE CONTROL COMPRISING EMPTY CHAMBERS - A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing target nucleic acid sequences, probes that each have a nucleic acid sequence for hybridization with a respective one of the target nucleic acid sequences to form a probe-target hybrid and emit a fluorescence signal in response to an excitation light, and, photosensors corresponding to each of the probes respectively, and, a control photosensor not corresponding to any of the probes such that the control photosensor does not sense the fluorescence signal of a corresponding probe-target hybrid during correct operation of the microfluidic device. | 12-22-2011 |
20110312784 | MICROFLUIDIC DEVICE FOR DETECTING TARGETS WITH PROBES, DETECTION PHOTODIODES AND A CALIBRATION PHOTODIODE - A microfluidic device for detecting target molecules in a sample, the microfluidic device having probes for reaction with the target molecules to form probe-target complexes, the probe-target complexes being configured to emit photons when excited, photodiodes for detecting photons emitted by the probe-target complexes and a calibration photodiode for exposure to a calibration source to generate a calibration signal, wherein during use, output from the photodiodes for detecting photons emitted by the probe-target complexes is calibrated by the calibration signal. | 12-22-2011 |
20110312785 | SPOTTING DEVICE FOR SPOTTING FIXED ARRAY OF LOCS - A spotting device for contactless spotting of lab-on-a-chip (LOC) devices with oligonucleotide probes, the LOC devices being held in a fixed array on a printed circuit board (PCB) and each having an array of hybridization chambers for receiving the oligonucleotide probes, the probes having nucleic acid sequences that are complementary to target nucleic acid sequences to be identified in a biological sample, the spotting device having a monolithic supporting substrate, an array of reservoirs on one side of the supporting substrate, the reservoirs containing sufficient amount of the oligonucleotide probes suspended in a liquid to spot all the LOC devices on the PCB, and, an array of ejectors on the other side of the supporting substrate, each of the ejectors being configured for fluid communication with a corresponding one of the reservoirs respectively such that the ejectors eject droplets containing the oligonucleotide probes from the corresponding reservoir into one of the hybridization chambers. | 12-22-2011 |
20110312786 | GENETIC ANALYSIS LOC WITH HYBRIDIZATION ARRAY WITH CALIBRATION CHAMBER CONTAINING PROBE DESIGNED TO BE NONCOMPLEMENTARY TO NUCLEIC ACID SEQUENCES IN THE AMPLICON - A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing a target nucleic acid sequences, a hybridization chamber containing probes each having a nucleic acid sequence for hybridization with the target nucleic acid sequences to form probe-target hybrids, and, a calibration chamber containing a control probe incapable of hybridization with any sequence in the biological sample. | 12-22-2011 |
20110312787 | LOC HAVING USB DEVICE DRIVER FOR USE IN A TEST MODULE TO CONTROL USB CONNECTION - A lab-on-a-chip (LOC) device for installation in a test module for analyzing a sample fluid and communicating test results to an external device, the LOC device having a supporting substrate, a sample inlet for receiving the sample, a microsystems technology (MST) layer with functional sections for processing and analyzing the sample, and, CMOS circuitry on the supporting substrate, the CMOS circuitry having a universal serial bus (USB) device driver for operative control of a USB plug in the test module for communication with the external device. | 12-22-2011 |
20110312788 | GENETIC ANALYSIS LOC WITH THERMAL BOILING-INITIATED VALVE - A lab-on-a-chip (LOC) device for genetic analysis having a supporting substrate, an array of probes for hybridization with target nucleic acid sequences to form probe-target hybrids, a channel for directing a flow of liquid containing the target nucleic acid sequences, the channel having an inlet, an outlet and a meniscus anchor between the inlet and the outlet such that the flow from the inlet towards the outlet stops at the meniscus anchor where the liquid forms a meniscus, and, an actuator valve with a valve heater for heating the meniscus such that the meniscus unpins from the meniscus anchor such that the liquid flow towards the outlet resumes. | 12-22-2011 |
20110312789 | LOC DEVICE WITH FLASH MEMORY - A lab-on-a-chip (LOC) device for analyzing a sample fluid, the LOC device having a supporting substrate, a sample inlet for receiving the sample, a microsystems technology (MST) layer with functional sections for processing and analyzing the sample, and, CMOS circuitry between the supporting substrate and the MST layer, the CMOS circuitry having flash memory for storing program data to operate the functional sections during processing and analysis of the sample. | 12-22-2011 |
20110312790 | MICROFLUIDIC TEST MODULE WITH LOW-VOLUME HYBRIDIZATION CHAMBER - A microfluidic test module having an outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, and, a hybridization chamber mounted in the casing, the hybridization chamber containing probes having a nucleic acid sequence for hybridization with the target nucleic acid sequence to form probe-target hybrids, wherein, the hybridization chamber has a volume less than 900,000 cubic microns. | 12-22-2011 |
20110312791 | TEST MODULE WITH FAULT-TOLERANT MULTIPLE VALVE ASSEMBLY - A test module for genetic analysis having a casing for hand held portability, an inlet for receiving a liquid containing nucleic acid sequences, an outlet downstream of the inlet, and, a fault-tolerant multiple valve assembly having a plurality of flow-paths extending from the inlet to the outlet and a plurality of valves positioned along each of the flow-paths respectively. | 12-22-2011 |
20110312792 | TEST MODULE THAT UPDATES EPIDEMIOLOGICAL DATABASES - A test module for analyzing a sample fluid and communicating epidemiological data to a database, the test module having a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the epidemiological database, and, a controller for operative control of the communication interface. | 12-22-2011 |
20110312793 | MICROFLUIDIC TEST MODULE WITH LOW MASS OF PROBES - A microfluidic test module having an outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, and, a hybridization chamber mounted in the casing, the hybridization chamber holding a volume of fluid containing probes, the probes each having a nucleic acid sequence for hybridization with the target nucleic acid sequence to form probe-target hybrids, wherein, the mass of the probes in each of the hybridization chambers is less than 270 picograms. | 12-22-2011 |
20110312794 | TEST MODULE THAT UPDATES EPIDEMIOLOGICAL DATABASES WITH LOCATION DATA - A test module for analyzing a sample fluid and communicating location data to an epidemiological database, the test module having a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the epidemiological database, and, a controller for operative control of the communication interface, wherein, the controller is configured to associate location data with epidemiological data sent to the communication interface for communication with the epidemiological database. | 12-22-2011 |
20110312795 | DIAGNOSTIC TEST MODULE WITH A LOC WITH INTEGRAL PHOTOSENSOR AND EXCITATION LED FOR DETECTION OF HYBRIDIZATION ASSAY RESULTS - A test module for detecting hybridization of probes with target nucleic acid sequences, the test module having a receptacle for receiving a biological sample with target nucleic acid sequences, an array of probes for hybridization with the target nucleic acid sequences, an excitation light emitting diode (LED) for illuminating the array of probes, CMOS circuitry having a photosensor for sensing a fluorescence emission from any of the probes in the array that have hybridized with the target nucleic acid sequences such that the CMOS circuitry is configured to use outputs from the photosensor to generate a signal indicative of the probes that have hybridized, wherein, the array of probes and the CMOS circuitry are integrated on a lab-on-a-chip (LOC) device contained within the test module. | 12-22-2011 |
20110312796 | TEST MODULE THAT UPDATES MEDICAL DATABASES - A test module for analyzing a sample fluid and communicating data to a medical database, the test module having a receptacle for receiving the sample, functional sections for processing and analyzing the sample, a communication interface for communication with the medical database, and, a controller for operative control of the communication interface. | 12-22-2011 |
20110312797 | PORTABLE TEST MODULE FOR FLUORESCENCE EXCITATION OF PROBE NUCLEIC ACID SEQUENCES - A test module for detecting hybridization of probes with target nucleic acid sequences, the test module having a casing configured for hand held portability, the casing having a receptacle for receiving a biological sample with target nucleic acid sequences, an array of probes for hybridization with the target nucleic acid sequences, an excitation light source for illuminating the array of probes, and a photosensor for sensing a fluorescence emission from any of the probes in the array that have hybridized with the target nucleic acid sequences. | 12-22-2011 |
20110312798 | TEST MODULE WITH INBUILT LANCET - A test module for processing and analyzing a blood sample, the test module having an outer casing with a receptacle for receiving blood, functional sections for processing and analyzing the sample, and, a lancet for collecting the blood sample from a patient. | 12-22-2011 |
20110312799 | USB-INTERFACEABLE PORTABLE TEST MODULE FOR DETECTION OF HYBRIDIZED PROBES - A test module for detecting hybridization of probes with target nucleic acid sequences, the test module having a receptacle for receiving a biological sample with target nucleic acid sequences, an array of probes for hybridization with the target nucleic acid sequences, an excitation light source for illuminating the array of probes, circuitry incorporating a photosensor for sensing a fluorescence emission from any of the probes in the array that have hybridized with the target nucleic acid sequences, wherein, the circuitry is configured to use outputs from the photosensor to generate a signal indicative of the probes that have hybridized and transmit the signal to an external device. | 12-22-2011 |
20110312800 | TEST MODULE FOR GRAVITY-INDEPENDENT OPERATION - A test module for analyzing genetic material in a biological sample, the test module having an outer casing with a receptacle for receiving the biological sample, functional sections for processing and analyzing the biological sample, a controller for operating the functional sections, and, a flow-path from the receptacle to the functional sections, wherein, the flow-path is configured to draw the sample to be analyzed from the receptacle to the functional sections by capillary action regardless of module orientation relative to gravity. | 12-22-2011 |
20110312801 | TEST MODULE WITH LANTHANIDE METAL-LIGAND COMPLEX FLUOROPHORE - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, and, probes in the outer casing for hybridization with the target nucleic acid sequence to form probe-target hybrids, the probes each having a fluorophore such that the probe-target hybrids emit a fluorescence signal in response to exposure to an excitation light, wherein, the fluorophore is a lanthanide metal-ligand complex. | 12-22-2011 |
20110312802 | TEST MODULE WITH PROBES SUSPENDED IN FLUID - A test module having an outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, and, probes in the outer casing for hybridization with the target nucleic acid sequence to form probe-target hybrids, the probes each having a fluorophore such that the probe-target hybrids emit a fluorescence signal in response to exposure to an excitation light, wherein, the probes are suspended in a fluid. | 12-22-2011 |
20110312803 | SYSTEM FOR VARIABLE LOADING OF REAGENTS INTO MICROFLUIDIC DEVICE FOR GENETIC ANALYSIS - A system for loading reagents into a microfluidic device for genetic analysis, the system having containers with reagents for processing a biological sample in the microfluidic device, an integrated circuit secured to each of the containers respectively, each of the integrated circuits having a container digital memory storing reagent specification data related to the reagent in that container, a microfluidic device for performing a desired genetic test assay, the microfluidic device having a device digital memory, a reagent dispensing apparatus for loading a selection of the reagents into the microfluidic device, the reagent dispensing apparatus having a control microprocessor for accessing the container digital memory and transmitting the specification data to the device digital memory, and, a device reader for accessing the reagent specification data from the device digital memory in order to analyze test assay results from the microfluidic device. | 12-22-2011 |
20110312804 | MICROFLUIDIC DEVICE WITH APERTURE WITH GEOMETRY TO PROMOTE UNPINNED FLOW-THROUGH OF FLUID - A microfluidic device having a channel having an inlet, an outlet and an aperture between the inlet and the outlet, wherein, the aperture has a geometry that does not allow a meniscus of the fluid flow to anchor to the aperture, such that fluid flow from the inlet towards the outlet is uninterrupted. | 12-22-2011 |
20110312805 | TEST MODULE WITH TIME DELAYED DETECTION OF FLUORESCENCE FROM HYBRIDIZED PROBE - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, control circuitry having a photosensor for generating an output signal in response to the fluorescence signal, wherein, the control circuitry is configured to initiate a time delay upon deactivation of the excitation light before activating the photosensor. | 12-22-2011 |
20110312806 | MICROFLUIDIC DEVICE WITH HUMIDIFIER - A microfluidic device for analyzing a fluid sample containing target molecules, the microfluidic device having a supporting substrate, a microsystems technologies (MST) layer on the supporting substrate for processing the fluid sample, the MST layer having an array of probes configured to combine with the target molecules to form probe-target complexes, and, a humidifier configured to heat water for localized humidification of an area encompassing the MST layer. | 12-22-2011 |
20110312807 | MICROFLUIDIC TEST MODULE WITH A MEMBRANE SEAL TO PREVENT DEHUMIDIFICATION OF THE MIXTURE - A microfluidic test module for analyzing genetic material, the test module having a casing configured for hand-held portability, an array of probes housed in the casing, the probes being configured to hybridize with target nucleic acid sequences to form probe-target hybrids, wherein, the casing has a membrane seal for reducing dehumidification within the casing and providing pressure relief from small air pressure fluctuations. | 12-22-2011 |
20110312808 | TEST MODULE WITH CONTROLLED EXPOSURE OF FLUOROPHORES TO EXCITATION LIGHT SOURCE - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, and, probes in the outer casing for hybridization having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, control circuitry having a photosensor for generating an output signal in response to the fluorescence signal, wherein, the control circuitry is configured to initiate a time delay upon deactivation of the excitation light before activating the photosensor. | 12-22-2011 |
20110312809 | TEST MODULE WITH HUMIDIFIER - A test module for analyzing genetic material, the test module having a casing configured for hand-held portability, an array of probes housed in the casing, the probes being configured to hybridize with target nucleic acid sequences to form probe-target hybrids, and, a humidifier for increasing the humidity within the casing. | 12-22-2011 |
20110312810 | SINGLE-USE TEST MODULE FOR DETECTION OF HYBRIDIZATION OF TARGETS WITH OLIGONUCLEOTIDE PROBES - A single-use test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, a photosensor to detect the fluorescence signal. | 12-22-2011 |
20110312811 | LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET SEQUENCES WITH ELECTRODES PROFILED FOR GREATER PERIPHERAL EDGE LENGTH - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, a photosensor for sensing the photons emitted from the ECL luminophore, wherein, the electrodes are plates of conductive material, the plates having edge profiles configured such that the length of peripheral edge of each of the plates is greater than 128 microns. | 12-22-2011 |
20110312812 | GENETIC TEST MODULE WITH FEEDBACK-CONTROLLED HUMIDIFIER - A test module for processing genetic material in a biological sample, the test module having a casing, a microfluidic device housed in the casing, the microfluidic device having reagents for processing the genetic material, a humidifier for increasing the humidity within the casing, a humidity sensor for sensing the humidity within the casing, wherein, the humidity sensor generates an output used for control of the humidifier. | 12-22-2011 |
20110312813 | SINGLE-USE GENETIC TEST MODULE - A single-use test module for detecting hybridization of probes with nucleic acid sequences, the test module having a sample input for receiving a biological sample with target nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the target nucleic acid sequences in the biological sample, an array of probes for hybridization with target nucleic acid sequences in the biological sample, and, circuitry having a photosensor for sensing hybridization between the probes and target nucleic acid sequences in the biological sample, wherein, the circuitry is configured to use outputs from the photosensor to generate a signal indicative of the probes that have hybridized. | 12-22-2011 |
20110312814 | SINGLE-USE TEST MODULE WITH EXCITATION SOURCE - A single-use test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, an excitation light source. | 12-22-2011 |
20110312815 | MICROFLUIDIC DEVICE WITH HUMIDITY SENSOR - A microfluidic device having a supporting substrate, a microsystems technologies (MST) layer on the supporting substrate for processing a fluid sample, and, a humidity sensor for sensing the humidity of an area encompassing the MST layer, wherein, the humidity sensor generates an output for humidity control of the area encompassing the MST layer. | 12-22-2011 |
20110312816 | TEST MODULE WITH LED FOR SIMULTANEOUS EXCITATION OF OLIGONUCLEOUTIDE PROBES - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, an excitation LED for generating the excitation light, the LED positioned in the outer casing for simultaneously illuminating all the probes. | 12-22-2011 |
20110312817 | MICROFLUIDIC TEST MODULE WITH HUMIDITY SENSOR - A test module for analyzing a sample containing nucleic acid sequences, the test module having a casing configured for hand-held portability, an array of probes housed in the casing, the probes being configured to hybridize with target nucleic acid sequences in the sample to form probe-target hybrids, a humidity sensor for sensing the humidity within the casing, wherein, the humidity sensor generates an output for humidity control within the casing. | 12-22-2011 |
20110312818 | TEST MODULE WITH EXCITATION LIGHT AND LENS FOR SIMULTANEOUS EXCITATION OF OLIGONUCLEOUTIDE PROBES - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, an excitation light for generating the excitation light, and, a lens positioned in the outer casing for directing light from the excitation light to simultaneously illuminate the probes. | 12-22-2011 |
20110312819 | LOC DEVICE FOR DETECTING TARGET NUCLEIC ACID SEQUENCES USING ELECTROCHEMILUMINESCENCE OF A LUMINOPHORE IN THE PRESENCE OF AN ELECTROCHEMICAL COREACTANT - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, an electrochemical coreactant that is present with the luminophore during electrochemiluminescence. | 12-22-2011 |
20110312820 | TEST MODULE WITH EXCITATION LIGHT AND PRISMS FOR SIMULTANEOUS EXCITATION OF OLIGONUCLEOUTIDE PROBES - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, an excitation light for generating the excitation light, and, prisms positioned in the outer casing for redirecting light from the excitation light to simultaneously illuminate the probes. | 12-22-2011 |
20110312821 | MICROFLUIDIC DEVICE WITH WASTE STORAGE - A microfluidic device having a supporting substrate, a microsystems technologies (MST) layer on the supporting substrate for analyzing a fluid, and, a waste reservoir for storage of waste material separated from the fluid. | 12-22-2011 |
20110312822 | GENETIC ANALYSIS LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET NUCLEIC ACID SEQUENCES - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having a supporting substrate, probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, CMOS circuitry between the supporting substrate and the probes for applying a voltage across the electrodes. | 12-22-2011 |
20110312823 | TEST MODULE WITH EXCITATION LIGHT AND MIRRORS FOR SIMULTANEOUS EXCITATION OF OLIGONUCLEOUTIDE PROBES - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, an excitation light for generating the excitation light, and, mirrors positioned in the outer casing for redirecting light from the excitation light to simultaneously illuminate the probes. | 12-22-2011 |
20110312824 | TEST MODULE WITH WASTE STORAGE INCORPORATING POROUS ELEMENT - A test module for analyzing fluid containing target nucleic acid sequences, the test module having a casing for hand-held portability, the casing having a receptacle for receiving the fluid, an array of probes for hybridization with target nucleic acid sequences to form probe-target hybrids, and, a waste accumulator for storage of waste material separated from the fluid, wherein, the waste accumulator has a waste reservoir and a porous element to draw the waste material into the waste reservoir by capillary action. | 12-22-2011 |
20110312825 | LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION WITH ELECTRODE PAIRS HAVING COMPLEMENTARY AND MUTUALLY INTERDIGITATED FINGER FORMATIONS - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, and, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, wherein, the electrodes have an anode and a cathode, each having fingers configured for mutual interdigitation. | 12-22-2011 |
20110312826 | TEST MODULE WITH LASER FOR EXCITATION OF OLIGONUCLEOUTIDE PROBES - A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization with a target nucleic acid sequence to form probe-target hybrids, the probe-target hybrids being configured to generate a fluorescence signal in response to an excitation light, a laser positioned in the outer casing for generating the excitation light. | 12-22-2011 |
20110312827 | GENETIC ANALYSIS LOC DEVICE FOR DETECTION OF TARGET SEQUENCES WITH ELECTROCHEMILUMINESCENT LUMINOPHORE AND FUNCTIONAL MOIETY FOR QUENCHING PHOTON EMISSIONS - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having a supporting substrate, probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, an electrochemiluminescent (ECL) luminophore, and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, CMOS circuitry between the supporting substrate and the probes for applying a voltage across the electrodes. | 12-22-2011 |
20110312828 | LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION USING ELECTRODE PAIRS OPTICALLY COUPLED TO PHOTODIODE - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrode pairs in which one electrode in each of the electrode pairs is a working electrode which causes oxidation or reduction of the luminophore to generate an excited species that emits a photon, and, photodiodes corresponding to each of the electrode pairs respectively, wherein, the photodiodes have a planar active surface area for receiving the light from the luminophore, and the working electrode has a surface area optically coupled to the active surface area of the photodiode, the working electrode being configured such that the optically coupled surface area is greater than 50% of the active surface area of the photodiode. | 12-22-2011 |
20110312829 | LOC DEVICE FOR GENETIC ANALYSIS AND ELECTROCHEMILUMINESCENT DETECTION OF TARGET SEQUENCES - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, and, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light. | 12-22-2011 |
20110312830 | GENETIC ANALYSIS LOC DEVICE WITH ELECTROCHEMILUMINESCENT PROBES HAVING A FUNCTIONAL MOIETY FOR QUENCHING PHOTON EMISSIONS CONFIGURED TO CHANGE PROXIMITY TO A LUMINOPHORE UPON FORMING A PROBE-TARGET HYBRID - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, an electrochemiluminescent (ECL) luminophore, and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, wherein during use, the functional moiety changes proximity to the luminophore upon formation of a probe-target hybrid. | 12-22-2011 |
20110312831 | LOC DEVICE FOR DETECTING TARGET NUCLEIC ACID SEQUENCE WITH ELECTROCHEMILUMINESCENT METALORGANIC COMPLEX - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having a supporting substrate, probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) metalorganic complex, and, electrodes for generating an excited state in the metalorganic complex in which the metalorganic complex emits photons of light, and, CMOS circuitry between the supporting substrate and the probes for applying a voltage across the electrodes. | 12-22-2011 |
20110312832 | LOC DEVICE FOR DETECTION OF TARGET SEQUENCES WITH ELECTROCHEMILUMINESCENT PROBES IN HYBRIDIZATION CHAMBERS - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, an array of hybridization chambers, each of the hybridization chambers having a pair of the electrodes respectively and containing a plurality of the probes, the nucleic acid sequence in the probes in each of the hybridization chambers being different to the nucleic acid sequence in at least one other hybridization chamber in the array such that a plurality of target nucleic acid sequences are detectable. | 12-22-2011 |
20110312833 | LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET SEQUENCES USING TRANSPARENT ELECTRODES - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, and, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, wherein, the electrodes are arranged in pairs, one of the electrodes in each electrode pair being a working electrode which causes oxidation or reduction of the luminophore to generate an excited species that emits a photon, the working electrode being transparent to light at the wavelength of the photons emitted by the ECL luminophores. | 12-22-2011 |
20110312834 | LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION USING A RUTHENIUM ORGANIC COMPLEX - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having a supporting substrate, probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and a ruthenium organic complex, electrodes for generating an excited state in the ruthenium organic complex in which the ruthenium organic complex emits photons of light, and, CMOS circuitry for applying a voltage across the electrodes. | 12-22-2011 |
20110312835 | GENETIC ANALYSIS LOC DEVICE WITH ELECTROCHEMILUMINESCENT PROBES AND INTEGRATED PHOTOSENSOR FOR DETECTION OF TARGET SEQUENCES - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, a photosensor for sensing the photons emitted from the ECL luminophore. | 12-22-2011 |
20110312836 | MICROFLUIDIC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET SEQUENCES - A microfluidic device for detecting target nucleic acid sequences in a sample, the microfluidic device having reagent reservoirs for adding reagents to the sample prior to detection of the target nucleic acid sequences, probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, and, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light. | 12-22-2011 |
20110312837 | LOC DEVICE FOR GENETIC ANALYSIS AND ELECTROCHEMILUMINESCENT DETECTION OF TARGET SEQUENCES - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, a photosensor for sensing the photons emitted from the ECL luminophore, wherein, the electrodes are plates of conductive material, the plates having peripheral edges optically coupled to the photosensor. | 12-22-2011 |
20110312838 | MICROFLUIDIC DEVICE WITH ELECTROCHEMILUMINESCENT PROBES AND INTEGRATED PHOTOSENSOR FOR DETECTION OF TARGET MOLECULES - A microfluidic device for detecting target molecules in a fluid, the microfluidic device having reagent reservoirs for adding reagents to the fluid prior to detection of the target molecules, probes for reaction with the target molecules to form probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, a photosensor for sensing the photons emitted from the ECL luminophore. | 12-22-2011 |
20110312839 | GENETIC ANALYSIS LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET SEQUENCES WITH WORKING ELECTRODE IN CONTACT WITH PHOTOSENSOR - A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and an electrochemiluminescent (ECL) luminophore, electrodes for generating an excited state in the ECL luminophore in which the ECL luminophore emits photons of light, and, a photosensor for sensing the photons emitted from the ECL luminophore, wherein, the electrodes have at least one working electrode which causes oxidation or reduction of the luminophore to generate an excited species that emits the photons, the working electrode being positioned immediately adjacent the photosensor. | 12-22-2011 |
20110312840 | MICROFLUIDIC DEVICE WITH SAMPLE INLET, ELECTROCHEMILUMINESCENT PROBES AND INTEGRATED PHOTOSENSOR FOR DETECTION OF TARGET SEQUENCES - A microfluidic device having a supporting substrate, a microsystems technologies (MST) layer with a hybridization section that has an array of electrochemiluminescent (ECL) probes for hybridization with target nucleic acid sequences in a fluid, and electrode pairs for receiving an electrical pulse, the ECL probes being configured to emit a photon of light when hybridized with one of the nucleic acid targets and activated by one of the electrodes, and, a photosensor for detecting photons of light from the ECL probes that have hybridized. | 12-22-2011 |
20110312841 | FABRICATION SYSTEM FOR LAB-ON-A-CHIP (LOC) DEVICES WITH DIFFERING APPLICATION SPECIFIC FUNCTIONALITY - A fabrication system for lab-on-a-chip (LOC) devices with differing application specific functionality, the fabrication system having a database of different functional categories, each of the functional categories having a plurality of functional section designs, means for selecting a compilation of the functional section designs to generate a LOC design in accordance with a specific functionality intended for LOC devices fabricated in accordance with the LOC design, and, a MST (microsystems technology) fabrication facility for fabricating LOC devices in accordance with the LOC design, wherein, the functional section designs in any one of the functional categories are functionally compatible with at least one of the functional section designs in the other functional categories. | 12-22-2011 |
20110312842 | OLIGONUCLEOTIDE SPOTTING DEVICE - An oligonucleotide spotting device for contactless spotting of oligonucleotide probes onto a surface, the probes having nucleic acid sequences that are complementary to target nucleic acid sequences to be identified in a biological sample, the oligonucleotide spotting device having a supporting substrate, an array of reservoirs for containing the oligonucleotide probes suspended in a liquid, and, an array of ejectors, each of the ejectors being configured for fluid communication with a corresponding one of the reservoirs respectively, wherein, the ejectors are configured to eject droplets containing the oligonucleotide probes from the corresponding reservoir onto the surface. | 12-22-2011 |
20110312843 | SPOTTING DEVICE FOR COMPLETE ASSAY SPOTTING OF LOCS - A spotting device for contactless spotting of a lab-on-a-chip (LOC) device with oligonucleotide probes, the LOC device having an array of hybridization chambers for receiving the oligonucleotide probes, the probes having nucleic acid sequences that are complementary to target nucleic acid sequences to be identified in a biological sample and the array of hybridization chambers being configured to hold a complete assay of oligonucleotide probes necessary for a predetermined analysis of the biological sample, the spotting device having an array of reservoirs for containing the oligonucleotide probes suspended in a liquid, and, an array of ejectors, each of the ejectors being configured for fluid communication with a corresponding one of the reservoirs respectively such that the ejectors eject droplets containing the oligonucleotide probes from the corresponding reservoir into one of the hybridization chambers, wherein, the array of reservoirs is configured to contain the complete assay of oligonucleotide probes necessary for the predetermined analysis to be performed by the LOC device. | 12-22-2011 |
20110312844 | BIOCHEMICAL DEPOSITION DEVICE - A biochemical deposition device for contactless deposition of biochemicals on a surface, the biochemical deposition device having an array of reservoirs for containing a plurality of biochemicals, and, an array of ejectors, each of the ejectors being configured for fluid communication with a corresponding one of the reservoirs respectively, wherein, the ejectors are configured to eject droplets containing the biochemical from the corresponding reservoir onto the surface. | 12-22-2011 |
20110312845 | OLIGONUCLEOTIDE SPOTTING DEVICE WITH LAMINAR STRUCTURE - An oligonucleotide spotting device for contactless spotting of oligonucleotide probes onto a surface, the probes having nucleic acid sequences that are complementary to target nucleic acid sequences to be identified in a biological sample, the oligonucleotide spotting device having an array of reservoirs for containing the oligonucleotide probes suspended in a liquid, an array of ejectors overlaying the array of reservoirs, each of the ejectors being configured for fluid communication with a corresponding one of the reservoirs respectively, wherein, the ejectors are configured to eject droplets containing the oligonucleotide probes from the corresponding reservoir onto the surface. | 12-22-2011 |
20110312846 | OLIGONUCLEOTIDE SPOTTING DEVICE WITH FLUIDICS ON BOTH SIDES OF SUPPORTING SUBSTRATE - An oligonucleotide spotting device for contactless spotting of oligonucleotide probes onto a surface, the probes having nucleic acid sequences that are complementary to target nucleic acid sequences to be identified in a biological sample, the oligonucleotide spotting device having a supporting substrate, an array of reservoirs on one side of the supporting substrate, the reservoirs configured for containing the oligonucleotide probes suspended in a liquid, an array of ejectors on the other side of the supporting substrate, and, a plurality of inlet channels for fluid communication between the reservoirs and the ejectors, wherein, the ejectors are configured to eject droplets containing the oligonucleotide probes from the corresponding reservoir onto the surface. | 12-22-2011 |
20110312847 | SPOTTING DEVICE WITH STORED OLIGONUCLEOTIDE SPECIFICATION DATA - A spotting device for contactless spotting of oligonucleotide probes onto a surface, the probes having nucleic acid sequences that are complementary to target nucleic acid sequences to be identified in a biological sample, the spotting device having an array of reservoirs for containing the oligonucleotide probes suspended in a liquid, an array of ejectors in fluid communication with the reservoirs, each having an actuator for ejecting droplets of liquid containing the oligonucleotide probes, and, CMOS circuitry for providing each of the drop ejection actuators with a drive pulse for droplet ejection, wherein, the CMOS circuitry has a digital memory. | 12-22-2011 |
20110312848 | OLIGONUCLEOTIDE SPOTTING DEVICE FOR WAFER-SCALE SPOTTING OF LOCS - An oligonucleotide spotting device for contactless spotting of a silicon wafer on which an array of lab-on-a-chip (LOC) devices are fabricated, the LOC devices being configured to use the oligonucleotide probes to detect target nucleic acid sequences in a biological sample and each having an array of hybridization chambers for receiving the oligonucleotide probes, the oligonucleotide spotting device having an array of reservoirs on one side of the supporting substrate, the reservoirs containing sufficient amount of the oligonucleotide probes suspended in a liquid to spot all the LOC devices on the wafer, and, an array of ejectors overlaying the array of reservoirs for fixed movement therewith, such that the ejectors eject droplets containing the oligonucleotide probes from the corresponding reservoir into one of the hybridization chambers. | 12-22-2011 |
20110312849 | OLIGONUCLEOTIDE SPOTTING DEVICE FOR EJECTING LOW VOLUME DROPLETS - An oligonucleotide spotting device for contactless spotting of probes onto a surface, the probes having nucleic acid sequences that are complementary to target nucleic acid sequences to be identified in a biological sample, the oligonucleotide spotting device having a monolithic supporting substrate, an array of ejectors formed on one side of the supporting substrate such that the ejectors eject droplets containing the oligonucleotide probes onto the surface, wherein during use, each of the ejectors in the array is configured to eject droplets having a volume less than 100 picoliters. | 12-22-2011 |
20110312850 | BIOCHEMICAL DEPOSITION DEVICE WITH HIGH DEPOSITION RATE - A biochemical deposition device for contactless deposition of biochemicals on a surface, the biochemical deposition device having a supporting substrate, an array of reservoirs on one side of the substrate, the reservoirs being configured for containing a plurality of biochemicals, and, an array of ejectors on the other side of the supporting substrate, each of the ejectors being configured for fluid communication with the reservoirs, wherein during use, the array of ejectors eject droplets containing the biochemicals onto the surface at a rate greater than 100 droplets per second. | 12-22-2011 |
20110312851 | DEVICE FOR HIGH DENSITY SPOTTING OF OLIGONUCLEOTIDES - An oligonucleotide spotting device for contactless spotting of probes onto a surface, the probes having nucleic acid sequences that are complementary to target nucleic acid sequences to be identified in a biological sample, the oligonucleotide spotting device having an array of ejectors, each having an actuator for ejecting droplets of liquid containing the probes, CMOS circuitry for providing each of the actuators with a drive pulse for droplet ejection, wherein, the array of ejectors is configured to spot the probes onto the surface at a density more than 1 probe spot per square millimeter. | 12-22-2011 |
20110312852 | ROBOTIC SYSTEM FOR LOADING OLIGONUCLEOTIDES INTO SPOTTING DEVICES - A robotic system for spotting oligonucleotides having an oligonucleotide spotting device for contactless spotting of oligonucleotides onto a surface, the oligonucleotide spotting device having an array of ejectors, each having a drop ejection actuator for ejecting droplets of liquid containing the oligonucleotides onto a surface, at least one reservoir in fluid communication with one or more of the ejectors and CMOS drive circuitry for providing each of the drop ejection actuators with a drive pulse for droplet ejection, and, an apparatus for loading oligonucleotides into the oligonucleotide spotting device, the apparatus having a stage for detachably mounting a plurality of the oligonucleotide spotting devices, and a plurality of oligonucleotide containers mounted for movement relative to the stage, each of the oligonucleotide containers having a droplet dispenser for ejecting droplets of fluid containing oligonucleotides into the reservoirs of the oligonucleotide spotting devices. | 12-22-2011 |
20110312853 | OLIGONUCLEOTIDE SPOTTING ROBOT - An oligonucleotide spotting robot for spotting oligonucleotide probes into a microfluidic device having a digital memory for data related to the oligonucleotide probes loaded into the microfluidic device, the oligonucleotide dispensing robot having an array of reservoirs for containing the oligonucleotide probes suspended in a liquid, an array of ejectors, each of the ejectors being configured for fluid communication with a corresponding one of the reservoirs respectively, a mounting surface for detachably mounting the microfluidic device for movement relative to the ejectors, and, a control processor for operative control of the ejectors and the mounting surface, wherein, the control processor is configured to activate the ejectors, move the ejectors selected for activation into registration with the microfluidic device and download the data to the digital memory. | 12-22-2011 |
20110312854 | OLIGONUCLEOTIDE SPOTTING ROBOT FOR SPOTTING ARRAYS OF LOCS - An oligonucleotide spotting robot for spotting oligonucleotide probes into an array of lab-on-a-chip (LOC) devices, each having a digital memory for data related to the oligonucleotide probes loaded into that LOC device, the oligonucleotide dispensing robot having an array of reservoirs for containing the oligonucleotide probes suspended in a liquid, an array of ejectors, each of the ejectors being configured for fluid communication with a corresponding one of the reservoirs respectively, a mounting surface for detachably mounting the array of LOC devices for movement relative to the ejectors, and, a control processor for operative control of the ejectors and the mounting surface, wherein, the control processor is configured to activate the ejectors, move the ejectors selected for activation into registration with one or more of the LOC devices and download the data specifically relevant to each of the LOC devices into the digital memory of that LOC device. | 12-22-2011 |
20110312855 | OLIGONUCLEOTIDE SPOTTING ROBOT FOR HIGH DENSITY SPOTTING OF OLIGONUCLEOTIDES - An oligonucleotide spotting robot for spotting oligonucleotide probes onto a substrate, the oligonucleotide dispensing robot having an array of reservoirs for containing the oligonucleotide probes suspended in a liquid, an array of ejectors, each of the ejectors being configured for fluid communication with a corresponding one of the reservoirs respectively, a mounting surface for detachably mounting the substrate for movement relative to the ejectors, and, a control processor for operative control of the ejectors and the mounting surface, wherein, the array of ejectors is configured to spot the probes onto the surface at a density more than 1 probe spot per square millimeter. | 12-22-2011 |
20110312856 | APPARATUS FOR DISPENSING REAGENTS, LOADING OLIGONUCLEOTIDE SPOTTING DEVICES AND SPOTTING OLIGONUCLEOTIDE PROBES - An apparatus for dispensing reagents, loading oligonucleotide spotting devices and spotting oligonucleotide probes, the apparatus having a plurality of reagent vials, each with a droplet dispenser, a plurality of oligonucleotide vials, each with a droplet dispenser, a mounting surface for detachably mounting a microfluidic device for movement relative to the reagent vials, and detachably mounting an oligonucleotide spotting device, a chuck for detachably mounting the oligonucleotide spotting device adjacent the mounting surface, and, a control processor for operative control of the reagent and oligonucleotide vials, the oligonucleotide spotting device when mounted in the chuck and movement of the mounting surface relative to the reagent and oligonucleotide vials, and the oligonucleotide spotting device, wherein, the control processor is configured to activate any of the droplet dispensers, move the microfluidic device into registration with the reagent vials and move the oligonucleotide spotting device into registration with the oligonucleotide vials. | 12-22-2011 |
20120004145 | OLIGONUCLEOTIDE SPOTTING ROBOT FOR WAFER-SCALE SPOTTING OF LOCS - An oligonucleotide spotting robot for spotting oligonucleotide probes into a silicon wafer on which an array of lab-on-a-chip (LOC) devices are fabricated, each LOC device having a digital memory for data related to the reagents loaded into the LOC device, the oligonucleotide dispensing robot having an array of reservoirs for containing the oligonucleotide probes suspended in a liquid, an array of ejectors, each of the ejectors being configured for fluid communication with a corresponding one of the reservoirs respectively, a mounting surface for detachably mounting the array of LOC devices for movement relative to the ejectors, and, a control processor for operative control of the ejectors and the mounting surface, wherein, the control processor is configured to activate the ejectors, move the ejectors selected for activation into registration with one or more of the LOC devices and download the data specifically relevant to each of the LOC devices into the digital memory of that LOC device. | 01-05-2012 |
20120028842 | GENETIC ANALYSIS LOC WITH HYBRIDIZATION ARRAY WITH POSITIVE CONTROL CHAMBERS INCORPORATING PROBES THAT HYBRIDIZE FOR ANY AMPLICON - A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing a target nucleic acid sequence, and, an array of probes each having a nucleic acid sequence for hybridization with the target nucleic acid sequence to form a probe-target hybrid, wherein, the array of probes includes a control probe for hybridization with a target sequence known to be always present in the biological sample. | 02-02-2012 |
20130079254 | Microfluidic dialysis device - A microfluidic dialysis device that has a first channel for receiving a biological sample, a second channel, and a series of adjacent channels extending between the first channel and the second channel. Each of the adjacent channels pin a meniscus of the sample that arrests capillary flow between the first channel and the second channel. A bypass channel between the first channel and the second channel, the bypass channel joining the second channel upstream of the adjacent channels and is configured for uninterrupted capillary driven flow from the first channel to the second channel. Flow from the bypass channel reaches the meniscus pinned at each of the adjacent channels after the meniscus has formed to sequentially remove each of the menisci and allow flow from the first channel to the second channel via the adjacent channels as well as the bypass channel. | 03-28-2013 |