42nd week of 2009 patent applcation highlights part 34 |
Patent application number | Title | Published |
20090258326 | Siwak tooth cleaning instrument - The siwak tooth cleaning instrument provides a carrying, protecting and application instrument for a siwak stick, to be applied to the user's teeth for cleaning thereof. In some embodiments, the siwak tooth cleaning instrument includes a tubular housing with a siwak that may be selectively and adjustably raised and lowered to extend the siwak from the housing for cleaning one's teeth, and retracted within the housing for storage and transport, similar to a lipstick. In other embodiments, the siwak tooth cleaning instrument has tooth cleaning cartridges including a siwak that releasably engage the head portion of an elongated handle. A cover may be pivotally attached to the handle for covering the handle and attached cartridge, or a storage tube with replacement cartridges may be pivotally attached to the handle. | 2009-10-15 |
20090258327 | SURFACE AREA OF A CERAMIC BODY AND CERAMIC BODY - Disclosed is a dental implant with a post element that can be inserted into a jawbone and with a mounting element attached to the post element, to which mounting element and dental element can be affixed with the post element designed as a ceramic body of yttrium and/or aluminum oxide stabilized zirconium oxide. Said dental implant should have an even additionally improved ingrowth or integration behaviour during the osseous implant healing, compared with the mentioned known concepts. According to the invention, the surface of the dental implant is provided with at least one partial area that has nanoscopic pore or an otherwise executed nanoscopic structure that has a depletion zone with a reduced yttrium and/or aluminum oxide element, compared with the internal volume. | 2009-10-15 |
20090258328 | 5 IN 1 DENTAL IMPLANT METHOD AND APPARATUS - A dental implant method and apparatus is disclosed. A dental implant may be placed during one surgery by extracting a tooth from a socket, drilling a hole through crestal bone at the top of the socket, dissecting sinus membrane from the crestal bone by pulsing water through the hole and separating the sinus membrane from the crestal bone, then inserting bone mixture through the hole and between the sinus membrane and the crestal bone to increase the thickness of crestal bone. Then, the hole may be bored to a first diameter and a dental implant having threads and a sidecut may be placed in the hole. After the dental implant is placed, gingival tissue may be vertically translated by dissecting the gingival tissue from alveolar bone near to the socket, making lateral cuts in the gingival tissue on the alveolar bone side of the gingival tissue, and translating the gingival tissue so it is adjacent to the dental implant. In this way, a dental implant may be implanted within one surgery. | 2009-10-15 |
20090258329 | DENTAL IMPLANT - The invention provides dental implants for fixed and removable prosthetic devices, and for other devices such as orthodontic devices, and has application to single tooth replacement, e.g., caps and crowns, and multiple tooth replacement using one or more implants, e.g., bridges, and multiple implants for full and partial prosthetic devices. Dental implants according to embodiments of the invention comprise a post or posts inserted into the jawbone and a base that is loaded, in the general area of the gum line, against the jawbone using the post or posts. According to some embodiments, the base is secured to each post, loaded against the jawbone, by a fastener system. | 2009-10-15 |
20090258330 | Method of Treating Dental Periapical Lesions - A method of treating a dental periapical lesion at an apex of a tooth root canal, by accessing the dental periapical lesion via the root canal; and removing the dental periapical lesion via the root canal. In the described preferred embodiments, the dental periapical lesion is accessed via an opening made in the tooth crown leading to the root canal; and the dental periapical lesion is removed by passing a rotary ablating device through the tooth crown, the root canal, and the apex, into engagement with the periapical lesion, rotating the ablating device to ablate the dental periapical lesion, and removing debris produced by the ablation of the periapical lesion via the root canal. | 2009-10-15 |
20090258331 | INTERACTIVE RECIPE PREPARATION USING INSTRUCTIVE DEVICE WITH INTEGRATED ACTUATORS TO PROVIDE TACTILE FEEDBACK - A user selects a recipe from a list of recipes. Preparation steps corresponding to the selected recipe are retrieved from a data store, such as a database. Each of the retrieved preparation steps is displayed sequentially to the user on a horizontal surface display that is included in an interactive cooking preparation device. During at least one preparation step, a selected kitchen appliance is identified, such as a configurable burner surface. The identified preparation step also includes one or more appliance parameters that correspond to the selected kitchen appliance. The selected kitchen appliance is controlled by sending one or more signals from the interactive cooking preparation device to the selected kitchen appliance. At least one of the signals sent to the appliance includes one of the appliance parameters. | 2009-10-15 |
20090258332 | INTERACTIVE RECIPE PREPARATION USING INSTRUCTIVE DEVICE WITH INTEGRATED ACTUATORS TO PROVIDE TACTILE FEEDBACK - An interactive cooking preparation device that includes a flexible horizontal display surface is presented. A set of actuators located under the flexible horizontal display surface that, when activated, cause a tactile sensation on the display surface. A user selects a recipe from a list of recipes. A set of preparation steps that correspond to the selected recipe are retrieved from a data store. Each of the preparation steps are displayed to the user on the flexible horizontal surface display. A current preparation step that is being performed by the user is identified. The user is directed to an area of the interactive cooking preparation device using a tactile feedback mechanism. The tactile feedback mechanism causes a physical sensation on the surface of the interactive cooking preparation device. The physical sensation is felt by the user when the user is touching the surface of the interactive cooking preparation device. | 2009-10-15 |
20090258333 | SPOKEN LANGUAGE LEARNING SYSTEMS - This invention relates to systems, methods and computer program code for facilitating learning of spoken languages. We describe a computing system to facilitate learning of a spoken language, the system comprising: a user interface to prompt a user of the system to produce a spoken language goal and to capture audio data comprising speech captured from said user in response; a speech analysis system to analyse said captured audio data to determine acoustic or linguistic pattern features of said captured audio data; a pattern matching system to match one or more subsets of said pattern features to a database of pattern features and to determine feedback data responsive to said match; and a feedback system to provide feedback to said user using said feedback data to facilitate said user to achieve said spoken language goal. | 2009-10-15 |
20090258334 | Human amalgamation ratiocination process sublimation system - A computer implemented process for evaluating a person's employment potential including the steps of: | 2009-10-15 |
20090258335 | Imaging system simulator - A medical training and simulation system includes a plurality of inanimate objects, each being associated with an identification tag which stores an identification code which uniquely identifies an associated object. A database stores information of the objects. A reader reads a respective identification code of a selected object. An information retrieving processor retrieves the stored information which corresponds to the read identification code of the selected object. A device reproduces at least a portion of the retrieved information of the selected object. | 2009-10-15 |
20090258336 | SYSTEM AND METHOD FOR DEVELOPMENT OF INTERPERSONAL COMMUNICATION - A method for facilitating communication between participants is provided. The method includes providing a plurality of individually-identifiable tokens to at least first and second participants, associating a unique question with each token, transferring a first token from the first participant to the second participant, asking the second participant the question that is associated with the first token, transferring a second token from the second participant to the first participant, and asking the first participant the question that is associated with the second token. The method further includes recording the answers that are provided by the first and second participants in response to the questions associated with the first and second tokens. | 2009-10-15 |
20090258337 | Thawed organ or tissue or thawed cell group to be donated, transplanted, added, or administered to living body, production process thereof, supercooled solution therefor, and production apparatus of the organ or tissue - The present invention aims to freeze and store organs or tissues taken from human bodies or animals and after thawing, use the resulting organs or tissues, or cells taken and extracted therefrom for medical treatment. The present invention is characterized in that the organs or tissues taken from human bodies or animals are regenerated through the steps of freezing, storing and thawing; the cells taken from the organs or tissues undergo cell division and colonization. | 2009-10-15 |
20090258338 | SIMULATED 5-PART DIFFERENTIAL LEUKOCYTE GRANULES, THE METHOD FOR PREPARING THE SAME AND CONTROL PRODUCTS AND CALIBRATORS COMPRISING THE SAME - A method for preparing five-part differential leukocyte analogs from red blood cells includes selecting appropriate red blood cells; treating the red blood cells with a multi-functional reagent system for maintenance of a structural integrity of the membranes of the red blood cells and synchronous adjustment of cell volume, morphology and inclusions; subjecting the treated red blood cells to an intensified fixation; and washing the treated cells for preservation. The present disclosure also relates to leukocyte analogs prepared by the above method, a reagent system used for preparing the analogs, and a quality control and a calibrator including the analogs for use in a hematology analyzer. | 2009-10-15 |
20090258339 | SYSTEMS, METHODS AND COMPOSITIONS FOR DETECTION OF HUMAN PAPILLOMA VIRUS IN BIOLOGICAL SAMPLES - The present invention comprises, without limitation, systems, methods, and compositions for the detection, identification, and quantification, down to the single copy level, of human papillomavirus (HPV) in biological samples, including but not limited to, mammalian body fluids and cervix scrapings, for purposes of detection, treatment and/or management of cancer and dysplasia. | 2009-10-15 |
20090258340 | Assay for SARS coronavirus by amplification and detection of the replicase sequence - Primers and probes derived from SARS-CoV nucleic acid that facilitate detection and/or quantification of the replicase gene are disclosed. The disclosed sequences may be used in a variety of amplification and non-amplification formats for detection of SARS-CoV infection. | 2009-10-15 |
20090258341 | Compositions and Methods for Detecting Bacteria - Genetically modified bacteriophage and methods of using the same to detect bacterial types of interest are provided. | 2009-10-15 |
20090258342 | OPTIMIZED PROBES AND PRIMERS AND METHODS OF USING SAME FOR THE DETECTION, QUANTIFICATION AND GROUPING OF HIV-1 - Described herein are optimized primers and probes useful for detecting, quantitating and grouping variant HIV-1 strains, and methods and kits of using the described primers and probes. | 2009-10-15 |
20090258343 | Semi-quantitative immunochromatographic device and method for the determination of HIV/AIDS immune-status via measurement of soluble CD40 Ligand/CD154, A CD4+ T cell equivalent and the simultaneous detection of HIV infection via HIV antibody detection - A semi-quantitative, immunochromatographic dual test device for the simultaneous detection of HIV/AIDS immune status CD4+ T cell equivalents, such as soluble CD40 ligand/CD 154, and the detection of an HIV antibody, includes one or more support materials capable of providing lateral flow. The one or more support materials include at least one sample receiving area for receiving a biological sample containing a first target analyte, the first target analyte being a CD4+ T cell equivalent, such as soluble CD40 ligand/CD 154, and a second target analyte, the second target analyte being an HIV antibody. A second area, situated on the one or more support materials, has a movably contained detector ligand and or detector antigens, wherein the detector ligand and or detector antigens is capable of forming a mobile complex with the soluble CD40 ligand/CD 154 and or HIV antibodies, and at least a first capture area having a predetermined amount of a first immobile capture reagent, the first immobile capture reagent capable of specifically binding to the mobile complex formed by the soluble CD40 ligand/CD 154 protein and the detector ligand and providing a visible signal. The one or more support materials further have situated thereon at least a second capture area having a predetermined amount of a second immobile capture reagent that is capable of specifically binding to HIV antibodies present in the biological sample and providing a visible signal. | 2009-10-15 |
20090258344 | METHODS FOR IDENTIFYING RISK OF BREAST CANCER AND TREATMENTS THEREOF - Provided herein are methods for identifying risk of breast cancer in a subject and/or a subject at risk of breast cancer, reagents and kits for carrying out the methods, methods for identifying candidate therapeutics for treating breast cancer, and therapeutic methods for treating breast cancer in a subject. These embodiments are based upon an analysis of polymorphic variations in nucleotide sequences within the human genome. | 2009-10-15 |
20090258345 | PROTEIN ASSOCIATED WITH COLORECTAL CANCER, POLYNUCLEOTIDE INCLUDING SINGLE-NUCLEOTIDE POLYMORPHISM ASSOCIATED WITH COLORECTAL CANCER, MICROARRAY AND DIAGNOSTIC KIT INCLUDING THE SAME, AND METHOD OF DIAGNOSING COLORECTAL CANCER USING THE SAME - Provided are an isolated nucleolar protein having an amino acid sequence of NCBI GenBank Accession No. XP_033371, a method of diagnosing colorectal cancer in an individual, including measuring an expression level of a protein having an amino acid sequence of NCBI GenBank Accession No. XP_033371 in the individual, and a polynucleotide for diagnosis or treatment of colorectal cancer including at least 10 contiguous nucleotides of a nucleotide sequence selected from the group consisting of nucleotide sequences of SEQ ID NOS: 1-5 and including a nucleotide at position 101 of the nucleotide sequence, or a complementary polynucleotide thereof. | 2009-10-15 |
20090258346 | Method of selecting sunflower genotypes with high oleic acid content in seed oil - The present invention relates to the selection of sunflower genotypes with high oleic acid content in seed oil. The invention concerns more particularly molecular markers useful for a rapid and easy selection of sunflower lines and then sunflower hybrids capable of producing seeds having high oleic acid content. | 2009-10-15 |
20090258347 | METHOD FOR DIAGNOSING AND MONITORING CELLULAR RESERVOIRS OF DISEASE - The invention provides an assay for diagnosing and/or monitoring a viral infection or disease in a patient, the assay including the steps of mixing a sample of leucocytes with a fluorescent cell membrane-permeable dye which stains RNA or both DNA and RNA within the leucocytes; identifying from all the leucocytes at least two of the three major sub-populations of leucoytes selected from the group consisting of monocytes, granulocytes and lymphocytes; determining the fluorescence intensity for each of the identified sub-populations; and comparing the fluorescence intensity of at least two cell sub-populations to each other to obtain at least one of the following ratios: monocytes:granulocytes, monocytes:lymphocytes, and granulocytes lymphocytes. The viral infection may be HIV and the disease may be AIDS. The invention also provides a method of monitoring the cellular viral, parasitic or bacterial reservoir of a patient with a viral or bacterial infection by the steps described above. A, kit for performing the assay or method is also provided. | 2009-10-15 |
20090258348 | ESTERASES FOR MONITORING PROTEIN BIOSYNTHESIS IN VITRO - The present invention relates to the use of an esterase for monitoring and/or tracking the synthesis of a protein, polypeptide or peptide in a cell-free translation system or in an in vivo expression system in which the synthesis of a protein, polypeptide or peptide can occur, wherein said monitoring and/or tracking comprises the detection of the function of said esterase. The present invention further relates to a vector comprising a nucleic acid molecule coding for an esterase and expressing an esterase fusionprotein. Moreover, the present invention relates to a vector comprising a nucleic acid molecule coding for an esterase and comprising in frame at least one multiple cloning site for a further protein/polypeptide/peptide, to be expressed in form of a fusion protein comprising said esterase (esterase activity) and said further proteinaceous peptide structure. The present invention also provides for a protein, polypeptide or peptide encoded by the vectors of the present invention. Additionally, the present invention relates to a kit comprising a vector of the present invention or a nucleic acid molecule as comprised by the vectors of the present invention. Also disclosed is a method for monitoring and/or tracking the synthesis of a protein, polypeptide or peptide in a cell-free translation system or in an in vivo expression system, comprising the step of detecting the function of an esterase. The present invention also teaches a method for immobilising a protein, polypeptide or peptide comprising the steps of (a) tagging said protein, polypeptide or peptide with an esterase and (b) binding said esterase to an esterase inhibitor, wherein said esterase inhibitor is immobilized on a solid substrate. Moreover, the present invention relates to uses of the vectors of the present invention or the nucleic acid molecules comprised therein for the preparation of a kit or for monitoring and/or tracking the synthesis of a protein, polypeptide or peptide in a cell-free translation system, whereby the monitoring and/or tracking comprises the detection of the function of said esterase. | 2009-10-15 |
20090258349 | METHOD FOR DETECTING AND QUANTIFYING PERCHLORATE-REDUCING BACTERIA - The present invention provides methods, compositions, and kits for detecting and quantitating perchlorate-reducing bacteria in samples using reagents that hybridize to and allow amplification of the pcrA gene. The invention includes a quantitative real-time PCR assay for amplification of the pcrA gene which may be used to detect and quantitate perchlorate-reducing bacteria in samples. | 2009-10-15 |
20090258350 | Diagnostic methods for determining prognosis of non-small cell lung cancer - The invention provides methods for identifying early stage non-small cell lung cancer (NSCLC) patients who will have a favorable prognosis for the recurrence of lung cancer after surgical resection. The invention is based on the discovery that assessment of chromosomal copy number abnormalities at two or more of chromosome 5p15, 7p12, 8q24 and centromere 6 can be used for prognostic classification. The invention preferably uses fluorescence in situ hybridization with fluorescently labeled nucleic acid probes to hybridize to patient samples to quantify the chromosomal copy number of the these genetic loci. Assessment of the copy number abnormality patterns using four classifiers produced statistically significant prognostic classification for NSCLC: (i) the Range3 pattern of cells showing a difference on a cell by cell basis, of at least three FISH probe signals between the FISH signals at the chromosomal locus with the largest number of FISH signals minus the FISH signals at the chromosomal locus with the lowest number of FISH signals; (ii) the MYC/EGFR % loss pattern assessing the percentage of cells showing fewer MYC FISH probe signals than EGFR FISH probe signals; (iii) a combination of the Range3 pattern and the MYC/CEP6 ratio pattern of a percentage of cells showing a relative loss of MYC FISH probe signals to the FISH probe signal for CEP6; (iv) the combination of the MYC/5p15 ratio pattern showing the relative ratio of MYC and 5p15 locus signals of ≧0.80 and the 5p15/CEP6 ratio pattern assessing percentage of cells having a relative ratio of 5p15 FISH probe signals to CEP6 FISH probe signals ≧1.1 versus MYC/5p15 ratio of <0.80 or 5p15/CEP6<1.1; and (v) a combination of the average range of probe signal differences of equal to or greater than about 2.5 with the Range3 pattern in a percentage of the cells. The invention can be used to identify those early stage NSCLC patients at higher risk of recurrence who should be treated with neoadjuvant chemotherapy before surgery or with adjuvant chemotherapy after surgery. | 2009-10-15 |
20090258351 | Methods and Reagents for Combined PCR Amplification - An oligonucleotide probe is disclosed, the probe including an oligonucleotide, a fluorescer molecule attached to a first end of the oligonucleotide and a quencher molecule attached to the opposite end of the oligonucleotide. The probe is rendered impervious to digestion by the 5′→3′ exonuclease activity of a polymerase and the 5′→3′ extension of by a polymerase. The invention also includes methods for performing combined PCR amplification and hybridization probing, one such method including the steps of contacting a target nucleic acid sequence with PCR reagents and an oligonucleotide probe as described above, and subjecting these reagents to thermal cycling. One preferred refinement of the above method further includes the addition of a strand displacer to facilitate amplification. Additional similar combined PCR hybridization methods are disclosed, such methods not requiring probes having their 5′ ends protected, wherein (i) the polymerase lacks 5′→3′ exonuclease activity, (ii) a 5′→3′ exonuclease inhibitor is included, and (iii) an exonuclease deactivation step is performed. | 2009-10-15 |
20090258352 | Pin1 as a marker for abnormal cell growth - Methods for the use of Pin1 as a marker of abnormal cell growth are disclosed. In one embodiment, the method includes detecting a level of Pin1 to stage an abnormal cell growth, such as breast or prostate cancer. In another embodiment, the method includes evaluating the efficacy of a treatment of an abnormal cell growth, such as cancer, by monitoring the levels of Pin1. In another embodiment, the method includes evaluating the extent of metastasis of abnormal cell growth, such as cancer. The levels of Pin1 can be protein levels or nucleic acid levels. | 2009-10-15 |
20090258353 | PCR primer capable of reducing non-specific amplification and PCR method using the PCR primer - The present invention relates to a PCR primer facilitating hot-start PCR by suppressing non-specific amplification at room temperature and at the same time capable of reducing significantly non-specific amplification by dominating the amplification of the PCR product rather than the amplification of the original template from the third PCR cycle, more precisely a PCR primer prepared by additionally inserting the reverse-complementary sequence to a certain region starting from the 5′-start site of the 5′-terminus of the original primer which is composed of priming sequence to anneal to a PCR template into the 5′-terminus of the original primer and a PCR method using the same. The primer of the present invention has a original primer sequence composed of priming sequence to anneal to a PCR template and an additional reverse-complementary sequence, which inserted into the 5′-terminus of the original primer, to a certain region starting from the 5′-start site of the 5′-terminus of the original primer sequence, suggesting that a template-specific sequence and its reverse-complementary sequence are included in the same primer. The present invention can improve PCR specificity by reducing non-specific amplification. | 2009-10-15 |
20090258354 | Methods for DNA Length and Sequence Determination - Methods for determining nucleic acid length and sequence variation are provided, for example, between an unknown sample and a reference sample. In various embodiments, a method amplifies one or more specific regions of an oligonucleotide molecule in a sample comprising oligonucleotide molecules to produce a sample of amplicons, the sample of amplicons comprising two or more different amplicons. In various embodiments, two or more specific regions are amplified. In various embodiments, the methods (i) denature the amplicons in the sample of amplicons to produce a first set of single-stranded amplicons and a second set of single-stranded amplicons, single stranded amplicons of the second set being complementary to the corresponding single stranded amplicons of the first set and (ii) subject at least the first and second set of single stranded amplicons to mass spectrometric analysis to obtain the masses of the amplicons in the first and second set of single-stranded amplicons. The masses of the amplicons are then used, at least ultimately in part, to determine nucleic acid length and sequence variation. | 2009-10-15 |
20090258355 | Nanoscale Clusters and Methods of Making Same - The present invention is a method of making a nanocluster. The method comprises providing a surface comprising at least one anchoring biomolecule, wherein the surface is in a solution; adding an initial recognition-nano-component to the solution wherein the initial recognition-nano-component comprises i) a nanoparticle and one specifically-bindable-biomolecule, or ii) a nanoparticle and two different types of specifically-bindable-biomolecules, wherein a biomolecule of the initial recognition-nano-component specifically binds to the anchoring biomolecule; and adding a releasing biomolecule to the solution, wherein the releasing biomolecule binds to the anchoring biomolecule with a greater binding strength than the anchoring biomolecule binds to the initial recognition-nano-component, or wherein the releasing biomolecule binds to the initial recognition-nano-component with a greater binding strength than anchoring biomolecule binds to the initial recognition-nano-component, thereby making a nanocluster. | 2009-10-15 |
20090258356 | Modulators of the transporter ABCD3 for treating acne or hyperseborrhea - An in vitro method for screening candidate compounds for the preventive or curative treatment of acne, includes the determination of the capacity of a compound to modulate the expression or the activity of the peroxisome membrane transporter 1 comprising a type 3 ATP-binding site of the sub-family D (ABCD3), and the use of modulators of the expression or activity of this transporter for the treatment of acne or skin disorders associated with a hyperseborrhea; methods for the in vitro diagnosis or prognosis of these pathologies are also described. | 2009-10-15 |
20090258357 | CYTOKINE RECEPTOR COMMON GAMMA CHAIN LIKE - The present invention relates to a novel human gene encoding a polypeptide which is a member of the Cytokine Receptor family. More specifically, the present invention relates to a polynucleotide encoding a novel human polypeptide named Cytokine Receptor Common Gamma Chain Like, or “CRCGCL.” This invention also relates to CRCGCL polypeptides, as well as vectors, host cells, antibodies directed to CRCGCL polypeptides, and the recombinant methods for producing the same. Also provided are diagnostic methods for detecting disorders related to the immune system, and therapeutic methods for treating diagnosing, detecting, and/or preventing such disorders. The invention further relates to screening methods for identifying agonists and antagonists of CRCGCL activity. | 2009-10-15 |
20090258358 | Methods for Diagnosing and Treating Kidney and Colorectal Cancer - Methods, reagents and kits for diagnosing and treating cancer such as kidney or colorectal cancer are disclosed. An immunoassay for detecting kidney or colorectal cancer is based on the relative change of the ADAMTSL4 protein in urine or blood compared with normal tissue. An immunohistochemical assay for detecting kidney or colorectal cancer is based on the relative absence of labeled antibody binding to cancerous tissue, compared with normal tissue. | 2009-10-15 |
20090258359 | MODULAR COMPOSITING-MULTIPLE LOT SCREENING PROTOCOLS FOR DETECTION OF PATHOGENS, MICROBIAL CONTAMINANTS AND/OR CONSTITUENTS - Provided are methods for sampling, testing and validating test lots, comprising: assembling a plurality of product portions from each of a plurality of test lots and combining the portions to provide a corresponding set of test lot samples; enriching the test lot samples; removing portions of each enriched sample, and combining the removed portions to provide a modular composite sample; and testing of the modular composite sample, and individual testing of the enriched test lot samples, using at least one suitable detection assay for a target microbe or organism, wherein when such testing is negative all test lots are validated, and wherein when such testing is positive with respect to the modular composite sample, or with respect to an individual enriched test lot sample, individual test lots may nonetheless yet be validated by further testing of a portion of respective initially-negative enriched test lot samples and obtaining negative results. | 2009-10-15 |
20090258360 | METHOD OF DETECTING EQUINE POLYSACCHARIDE STORAGE MYOPATHY - The present invention relates to diagnosing Polysaccharide Storage Myopathy (PSSM) disease in equines. | 2009-10-15 |
20090258361 | VARIANT TLR4 NUCLEIC ACID AND USES THEREOF - The invention provides methods to identify polymorphisms at the human TLR4 locus, as well as methods to identify individuals at risk of indications that increase their morbidity and mortality. | 2009-10-15 |
20090258362 | RNA Bioassay - The present invention relates to methods for characterizing the pathological state of the kidney, methods of characterizing an agent, methods of evaluating the kidney protective and therapeutic characteristics of an agent and methods of monitoring the effect of a pharmaceutical agent on the kidney of a subject, related to measuring a kidney RNA marker in the urine of a subject. | 2009-10-15 |
20090258363 | Targeted integration and expression of exogenous nucleic acid sequences - Disclosed herein are methods and compositions for targeted integration of a exogenous sequence into a predetermined target site in a genome for use, for example, in protein expression and gene inactivation. | 2009-10-15 |
20090258364 | Method of determining the sensitivity of cancer cells to EGFR inhibitors including cetuximab, panitumumab and erlotinib - A method of determining if a cancer patient is amenable to treatment with EGFR inhibitors including but not limited to cetuximab, panitumumab or erlotinib by the following steps:
| 2009-10-15 |
20090258365 | METHOD FOR DETECTING IGF1R/Chr 15 in CIRCULATING TUMOR CELLS USING FISH - The present invention describes methods and probe composition for an automated FISH assay of a blood sample containing circulating tumor cells expressing the IGF-1R gene. The assay provides genetic analysis of suspect circulating tumor cells that have been identified after immunomagnetic selection and fluorescent labeling. Using unique, repeat-free probes to the IGF-1R locus and a chromosome 15 reference probe, cell lines expressing an aberrant number of IGF-1R and Chr 15 signals were detected, including one cell line with a low level of IGF-1R amplification. The ability to directly examine the genetic profile of IGF-1R on circulating tumor cells may provide an automated means for assessing disease and patient response to therapy. | 2009-10-15 |
20090258366 | pCryptoRNAi - A vector developed to transform fungi can be used to study the expression of a gene of interest. The vector can provide for the expression of signal proteins in fungi that can be observed and/or monitored. The vector can be used to investigate the effects of RNA interference on a gene of interest in pathogenic fungi. Systems and methods of using the vector are provided. | 2009-10-15 |
20090258367 | METHOD OF MAPPING OF mRNA DISTRIBUTION WITH ATOMIC FORCE MICROSCOPY - The present invention relates to a method of mapping of mRNA distribution, comprising the steps of a preparing a probe DNA attached to a apical liner region of the dendron on AFM cantilever where the probe DNA can specifically hybridize a target mRNA and measuring specific adhesive force between the probe DNA and the target mRNA on sectioned tissue at nanometer resolution. | 2009-10-15 |
20090258368 | PARAMAGNETIC NUCLEATION NANOPARTICLES - A nucleic acid binding substance which has affinity for nucleic acid polymers. The nucleic acid binding substance is comprised of a nucleic acid binding element capable of specific binding to nucleic acid molecules and connected to a nucleation nanoparticle having paramagnetic properties. | 2009-10-15 |
20090258369 | OVER-EXPRESSION AND MUTATION OF A TYROSINE KINASE RECEPTOR FGFR4 IN TUMORS - This disclosure provides tyrosine kinase protein and nucleic acid variants, particularly FGFR4 variants, which are linked to increased risk of tumor metastasis. The disclosure further provides methods of diagnosis and prognosis, and development of new therapeutic agents using these molecules and fragments thereof, and kits for employing these methods and compositions. | 2009-10-15 |
20090258370 | Acute Myelogenous Leukemia Biomarkers - The present invention provides novel compositions and their use in classifying acute myelogenous leukemia. | 2009-10-15 |
20090258371 | METHOD OF DETECTING VERY LOW LEVELS OF ANALYTE WITHIN A THIN FILM FLUID SAMPLE CONTAINED IN A THIN THICKNESS CHAMBER - A method and apparatus for the detection and quantification of very low levels of a target analyte using an imaging system is provided. In the case of some analytes such as certain hormones, for example TSH, their levels may be as low as several tens of thousands of molecules per micro liter. These extremely low levels can be measured by using the present invention to count the individual molecules of analyte. The invention also has the advantage of being a primary quantitative method, which is one which needs no standardization. | 2009-10-15 |
20090258372 | Detection Systems Utilizing Supported Lipid Bilayers - The invention relates to lipid bilayer coated beads and methods of using those beads in immunoassays, in analytical assay and the like. | 2009-10-15 |
20090258373 | METHODS OF CONTROLLING THE SENSITIVITY AND DYNAMIC RANGE OF A HOMOGENEOUS ASSAY - A method is disclosed for accurately determining the concentration of a target analyte utilizes reagent pairs having different affinity for the target. The different affinity provides distinct binding profiles that can be analyzed to absolutely determine the analyte concentration. The method provides an assay system having expanded dynamic range to cover a wider range of analyte concentration and can overcome the hook-effect that commonly exists in homogenous assay systems. The method utilizes distinguishable signals that allows for the analysis of multiple binding profiles and multiplex analysis. | 2009-10-15 |
20090258374 | Multisignal Labeling Reagents, and Processes and Uses Therefor - The present invention provides multisignal labeling reagents and these are useful in a number of biochemical applications, including the manufacture of biomolecular probes and their use in detecting or amplifying analyte-specific moieties. | 2009-10-15 |
20090258375 | ASSAYS FOR DETECTING PREGNANCY-ASSOCIATED GLYCOPROTEINS - Disclosed are methods and compositions for detecting pregnancy in an animal by means of assaying peptidase activity of one or more Pregnancy Associated Glycoproteins (PAGs). In certain aspects, methods also comprising use of an antibody that binds immunologically to a PAG that displays proteolytic activity are provided. Substrates of proteolytic PAGs are also provided, as are kits, and methods of use. Further, methods of purifying PAGs based on their proteolytic activity are also provided. | 2009-10-15 |
20090258376 | Protein isoforms and uses thereof - There is provided a method for screening for or, diagnosis or prognosis of a neurological disorder in a subject, for determining the stage or severity of such a neurological disorder in a subject, for identifying a subject at risk of developing such a neurological disorder, or for monitoring the effect of therapy administered to a subject having such a neurological disorder, said method comprising:
| 2009-10-15 |
20090258377 | Method of Measuring Enzymatic Activity of Adsorbed Allergenic Enzyme - The invention relates to a method of measuring the immunological activity of a vaccine preparation in the form of a mixture of one or more allergenic enzyme(s) and an oxygen-containing metal salt adjuvant, wherein the mixture comprises a liquid phase and a solid phase, and wherein at least a part of the allergenic enzyme(s) is adsorbed to the solid phase, the method comprising the steps of measuring the enzymatic activity of the mixture in an enzyme activity assay, and using the measurement obtained as an indication of the immunological activity of the vaccine preparation, or using the measurement obtained for quantifying the amount of allergenic enzyme. | 2009-10-15 |
20090258378 | STRESS-INDUCED PHOSPHOPROTEIN 1 AS A BIOMARKER FOR THE DETECTION OF HUMAN OVARIAN CANCERS AND ENDOMETRIOSIS - Disclosed herein is a method for the detection, preliminary screening or monitoring of a gynecological disease selected form ovarian cancers and endometriosis, in which STIP1 is used as a biomarker for the gynecological disease. | 2009-10-15 |
20090258379 | ISOLATION OF MEMBRANE VESICLES FROM BIOLOGICAL FLUIDS AND METHODS OF USING SAME - Methods of isolating membrane vesicles from a biological fluid sample are provided. In some embodiments, the methods comprise providing a biological fluid sample comprising membrane vesicles; filtering the biological fluid sample through a filtration module comprising a filter having an average pore diameter of between about 0.01 um and about 0.15 um; and collecting from the filtration module a retentate comprising the membrane vesicles, thereby isolating the membrane vesicles from the biological fluid sample. | 2009-10-15 |
20090258380 | Perhydrolase Epitopes - The present invention provides perhydrolase enzyme CD4+ T-cell epitopes, as well as variants that exhibit reduced immunogenic responses, as compared to the parental perhydrolase . The present invention further provides DNA molecules that encode perhydrolase variants, and host cells comprising DNA encoding perhydrolase variants, as well as methods for making perhydrolase enzymes less immunogenic. In addition, the present invention provides various compositions that comprise perhydrolase variants that are less immunogenic than the wild-type perhydrolase. In some specific embodiments, the present invention provides perhydrolase variants with reduced immunogenicity identified and/or characterized using the methods of the present invention. These enzymes find use in cleaning and other applications. In some preferred embodiments, the present invention finds particular use in applications involving cleaning, bleaching and disinfecting. | 2009-10-15 |
20090258381 | Methods for Determining the Cleavability of Substrates - The invention relates to methods for examining the enzymatic cleavability of substrates. In the methods, compounds, which have a cleavability of the section to be examined, are firstly synthesized on a first solid phase, separated therefrom, the cleavage reaction is carried out in solution and the cleaved and uncleaved compounds are immobilized on a second solid phase and the cleavability is determined. | 2009-10-15 |
20090258382 | Novel devices for the detection of the presence and/or activity of proteases in biological samples - The subject invention provides novel devices and methods for the detection of the presence and/or activity of proteases in biological samples. | 2009-10-15 |
20090258383 | OPTO-FLUIDIC ARCHITECTURE FOR PARTICLE MANIPULATION AND SORTING - This invention provides an apparatus for particle sorting, particle patterning, and methods of using the same. The sorting or patterning is opto-fluidics based, in that particles are applied to individual chambers in the device, detection and/or analysis of the particles is carried out, such that a cell or population whose removal or conveyance is desired is defined, and the cell or population is removed or conveyed via application of an optical force and flow-mediated conveyance or removal of the part. | 2009-10-15 |
20090258384 | Direct Antimicrobial Susceptibility Assay - An antimicrobial susceptibility assay, including the steps of: providing an assay dish; providing a growth medium in said dish; providing an antimicrobial agent sample; providing an interpretive indication located a predetermined distance from a sample location adjacent the growth medium in the assay dish; providing an interfitting element which interfits with said dish, said interfitting element configured to enable at least one the steps of: a) more accurately positioning the antimicrobial sample at the sample location in contact with the growth medium; b) providing the interpretive indication at a predetermined distance from said sample position to enable said interpretative indication to be compared with a margin of a zone of inhibition of a colony grown on said medium to determine an assay result, wherein said result can include determination of at least one of: a) “susceptible;” “intermediate;” and, “resistant;” placing a microorganism on the growth medium; placing the antimicrobial agent sample at said sample location with accuracy using said interfitting element; incubating said microorganism for a period sufficient to allow a margin of a zone of inhibition to be discernable; and, comparing the location of the margin of the zone of inhibition to the interpretive indication, to obtain a result including a determination of at least one of a) “susceptible;” b) “intermediate;” and, c) “resistant” for said microorganism with respect to the antimicrobial agent. | 2009-10-15 |
20090258385 | METHOD OF ANALYZING CELLS BY FLOW CYTOMETRY, USE OF AN ANTIOXIDANT AND/OR FREE RADICAL-SCAVENGING AGENT IN SUCH A METHOD AND INCUBATION BUFFER COMPRISING AN ANTIOXIDANT AND/OR FREE RADICAL-SCAVENGING AGENT - The invention relates to a method of analyzing cells by flow cytometry and the use of an antioxidant and/or free radical-scavenging agent in such a method. It also relates to an incubation buffer comprising an antioxidant and/or free radical-scavenging agent. The method of the invention comprises a step for labeling cells in a labeling solution comprising an incubation buffer and the labeling antibody or antibodies conjugated with at least one fluorochrome which is an APC tandem and an antioxidant and/or free radical-scavenging agent. The invention finds application in the field of the analysis of cells by flow cytometry, in particular. | 2009-10-15 |
20090258386 | METHODS FOR SOLID MUTAGENESIS AND SEMI-SOLID FLUID MUTAGENESIS FERMENTATION AND PURIFICATION OF LIPID SOLUBLE VITAMINS AND NUTRIENTS - According to the invention, Applicants have demonstrated methods for improving industrial biosynthesis of lipid soluble vitamins and nutrients. Applicants have also provided methods for cost-efficient and commercially-viable chemotherapeutic biosynthesis and purification. This invention provides novel methods for both solid mutagenesis and semi-solid fluid mutagenesis fermentation and the purification of lipid soluble vitamins and nutrients and increasing fermentation solid yields. | 2009-10-15 |
20090258387 | BIOACTIVE WHEY PROTEIN HYDROLYSATE - The invention relates to a partial hydrolysate of when protein which contains bioactive peptides but does not have a bitter flavour. The hydrolysate is carried out using selective enzymes which produce the active peptides and is terminated at a degree of hydrolysis before substantial bitter flavours are created. There are also described novel peptides and a method of reducing systolic blood pressure through the administration of the peptides. | 2009-10-15 |
20090258388 | METHODS OF ENHANCED HETEROLOGOUS PROTEIN SECRETION - A method of enhancing heterologous protein secretion in a yeast cell is disclosed. In one embodiment, the method comprising the steps of engineering a yeast cell to overexpress at last one gene selected from the group consisting of CCW12, CWP2, SED1, RPP0, ERO1 and their homologs, supplying the yeast cell with a nucleic acid encoding a heterologous protein and obtaining increased expression of the heterologous protein, wherein the expression is increased relative to the protein expression in a yeast cell that does not overexpress a gene selected from the group consisting of CCW12, CWP2, SED1, RPP0, ERO1 and their homologs. | 2009-10-15 |
20090258389 | Construction of New Variants of Dextransucrase DSR-S by Genetic Engineering - The present invention relates to a recombinant process for the production of truncated and/or mutated dextransucrases while conserving their enzymatic activity and/or their specificity in the synthesis of the α-1,6 bonds. More precisely, the present invention relates to nucleic acid sequences of truncated and/or mutated dextransucrases, vectors containing said nucleic acid sequences and host cells transformed by sequences encoding truncated and/or mutated dextransucrases. In a further aspect, the invention concerns a method for producing, in a recombinant manner, truncated and/or mutated dextransucrases which conserve their enzymatic activity and/or which conserve their specificity in the synthsis of α-1,6 bonds and however can produce, from saccharose, dextrans with high molar mass and with modified rheological properties, compared with the properties of dextran obtained with the native enzyme in the same conditions and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and IMO of the invention can be used namely as texturing agents or as prebiotics. | 2009-10-15 |
20090258390 | Prokaryotic collagen-like proteins and uses thereof - The present invention provides recombinant triple helical proteins or collagen-like proteins comprising a prokaryotic protein or one or more domains of a prokaryotic protein comprising a collagen-like peptide sequence of repeated Gly-Xaa-Yaa triplets and, optionally, one or more domains from a mammalian collagen. Also provided are expression vectors and host cells containing the expression vectors to produce these recombinant proteins and methods of producing the same. Additionally, antibodies are provided that are directed against a recombinant collagen-like protein that, preferably, binds an integrin. Furthermore, a method of screening for potential therapeutic compounds that inhibit the integrin-binding or integrin-interacting activities of recombinant collagen-like proteins. | 2009-10-15 |
20090258391 | RECOMBINANT CELL CLONES HAVING INCREASED STABILITY AND METHODS OF MAKING AND USING SAME - Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones. | 2009-10-15 |
20090258392 | SEQUENCE DIVERSITY GENERATION IN IMMUNOGLOBULINS - Compositions and methods are disclosed for generating immunoglobulin structural diversity in vitro, and in particular, for reducing biases in V region and J segment gene utilization, and for generating immunoglobulin V-D-J recombination events in a manner that does not require D-J recombination to precede V-DJ recombination. Selection of advantageous combinations of immunoglobulin gene elements, including introduction of artificial diversity (D) segment genes and optimization of recombination signal sequence (RSS) efficiency, are disclosed. | 2009-10-15 |
20090258393 | SINGLE CHAIN CLASS I MAJOR HISTOCOMPATIBILITY COMPLEXES, CONSTRUCTS ENCODING SAME AND METHODS OF GENERATING SAME - Provided are methods of generating a functional mammalian single chain MHC class I complex in prokaryotic expression systems and a host cell transformed with expression construct(s) capable of expressing a functional human single chain MHC class I complex capable of presenting specific antigenic peptides restricted to specific CTL clones. | 2009-10-15 |
20090258394 | METHOD FOR THE PRODUCTION OF HIGH-LEVEL SOLUBLE HUMAN RECOMBINANT INTERFERON ALPHA IN E. COLI AND VECTORS USEFUL FOR SUCH A PRODUCTION - A method for the production of high-level soluble human recombinant interferon alpha protein (rhuIFNα) in | 2009-10-15 |
20090258395 | IRX-2 MODIFIED MANUFACTURING PROCESS - A highly efficient method of making a primary cell derived biologic by purifying mononuclear cells (MNCs) in a automated cell processor to remove contaminating cells by loading leukocytes onto lymphocyte separation medium (LSM) and centrifuging the medium to obtain purified MNCs, storing the MNCs overnight in a closed sterile bag system, stimulating an induction mixture of the MNCs with phytohemagglutinin (PHA) or other mitogen and ciprofloxacin in a scalable cell culture device and producing a primary cell derived biologic from the MNCs, removing the mitogen from the induction mixture by filtering, incubating the induction mixture, clarifying the induction mixture by filtering to obtain a primary cell derived biologic supernatant, and clearing the primary cell derived biologic supernatant from adventitious agents by anion exchange chromatography, filtration. A closed system prevents contamination of the resulting primary cell derived biologic. An automated method of purifying cells. A method of scalably inducing cells. | 2009-10-15 |
20090258396 | CRUSTACEANS AS PRODUCTION SYSTEMS FOR THERAPEUTIC PROTEINS - A crustacean or rotifer is infected with a recombinant infectious virus that expresses a protein exogenous to the virus. The genome of the crustacean or rotifer itself remains unaltered. Crustacean, rotifer, insect, or viral promoters drive the transcription of a gene inserted into the recombinant virus genome, and the virus replicates in the crustacean or rotifer cell cytoplasm. The infected crustacean or rotifer can be provided directly to humans or non-human animals, or, following production and harvest of the crustaceans or rotifers, purified recombinant protein or polypeptide can be provided. Large quantities of biopharmaceuticals can be produced rapidly and inexpensively using this production system. | 2009-10-15 |
20090258397 | METHOD OF PRODUCING MENINGOCOCCAL MENINGITIS VACCINE FOR NEISSERIA MENINGITIDIS SEROTYPES A, C, Y, and W-135 - Methods for producing quadrivalent meningococcal meningitis polysaccharide and conjugate vaccines for serotypes A, C, Y and W-135 disclosed. | 2009-10-15 |
20090258398 | Process for the Production of beta-Amino Acids Using Acylase - The present invention relates to a | 2009-10-15 |
20090258399 | METHOD FOR PRODUCTION OF AMINO ACID - According to the present invention, there are provided a microorganism belonging to Enterobacteriaceae wherein a function of CsrB RNA or CsrC RNA has been decreased or lost, and which has the ability to produce and accumulate an amino acid, and a process wherein the microorganism is cultured in a medium to produce and accumulate the amino acid in the culture, and the amino acid is recovered from the culture. | 2009-10-15 |
20090258400 | Mutant Tyrosine Repressor, a Gene Encoding the Same, and a Method for Producing L-Dopa - A mutant tyrosine repressor that does not require tyrosine to induce expression of tyrosine phenol-lyase gene is obtained by introducing a mutation into a tyrosine repressor. A microorganism which is able to express large amounts of tyrosine phenol-lyase is obtained by introducing the mutant tyrosine repressor into the microorganism. The microorganism is useful for producing L-DOPA. | 2009-10-15 |
20090258401 | L-AMINO ACID PRODUCING BACTERIUM AND METHOD OF PRODUCING L-AMINO ACID - L-amino acid is produced by culturing a bacterium belonging to the Enterobacteriaceae family which has L-amino acid-producing ability and is modified so that expression of the nhaA gene, nhaB gene, nhaR gene, chaA gene, mdfA gene, or combinations thereof is enhanced. | 2009-10-15 |
20090258402 | L-Glutamic Acid Producing Bacterium and a Method for Production of L-Glutamic Acid - A method for producing L-glutamic acid by culturing a coryneform bacterium which has L-glutamic acid producing ability and which has been modified so that expression of the fasR gene is enhanced in a medium to produce and accumulate L-glutamic acid in the medium or cells, and collecting L-glutamic acid from the medium or cells. | 2009-10-15 |
20090258403 | NOVEL ALDOLASE, AND METHOD FOR PRODUCING OPTICALLY ACTIVE IHOG AND MONATIN - The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and aldolase used for these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid). | 2009-10-15 |
20090258404 | PRODUCTION OF FERMENTATION PRODUCTS IN BIOFILM REACTORS USING MICROORGANISMS IMMOBILISED ON STERILISED GRANULAR SLUDGE - Production of fermentation products, such as ethanol and lactic acid in biofilm reactors by microorganisms immobilised on sterilised granular sludge. | 2009-10-15 |
20090258405 | Preparation of optically active alcohols with whole-cell catalysts - The present invention relates to a process for the preparation of optically active alcohols from ketones with the aid of whole-cell catalysts comprising an alcohol dehydrogenase and also an enzyme capable of cofactor regeneration, a substrate concentration of at least 500 mM of ketone being provided for the conversion and the conversion being carried out without the addition of an “external” cofactor. | 2009-10-15 |
20090258406 | Use of esterases for separating plastics - The invention relates to agents containing esterases, and to the use thereof for dressing fibres, in particular, artificial fibres, washing and cleaning agents comprising esterases and corresponding washing and cleaning methods, in addition to additional technical areas of application. The invention also relates to the use of esterases for protecting against or reducing and/or preventing pilling, preferably in textiles, particularly plastic fibres, more preferably polyester fibres, in addition to the use of esterases for separating the plastics, in particular, polyester compounds. The invention further relates to novel esterases and to sufficiently related proteins and to derivatives thereof, agents containing them and to the use thereof. | 2009-10-15 |
20090258407 | TEMPERATURE TRANSFER DEVICES - A portable temperature transfer device for transferring thermo energy to and/or from a laboratory culture plate is provided as well as its methods of use. The temperature transfer device comprises a base and a raised stage that comprises a thermal conductive material. The raised stage allows direct contact between individual wells of the laboratory culture plate and the temperature transfer device. | 2009-10-15 |
20090258408 | Method for the isolation of spiroplasma in mammals - The present invention is directed to a method of isolating and culturing spiroplasma by obtaining a tissue sample from a bovine; inoculating embryonated eggs with the tissue sample; mixing fluid from the inoculated egg with culture media; culturing the media mixed with the egg fluid at approximately 32° C. for preferably greater than a month to establish a spiroplasma culture. | 2009-10-15 |
20090258409 | PURIFIED SR-P70 PROTEIN - The invention relates to new nucleic acid sequences of the family of tumor-suppressing genes related to the gene for the p53 protein, and to corresponding protein sequences. | 2009-10-15 |
20090258410 | METHODS FOR PRODUCING SOLUBLE MEMBRANE SPANNING PROTEINS - Methods for producing membrane-spanning polypeptides in high yields, with native conformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents, as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in bacterial cell hosts. | 2009-10-15 |
20090258411 | CENTRIFUGAL DEVICE AND METHOD FOR OVA DETECTION - A centrifugal device and method are provided for the separation of buoyant material such as parasitic ova from fecal matter. A rotor assembly, rotatable about its central axis in a centrifuge, includes a housing with a centrally located top opening leading to a centrally located mixing chamber. An annular sediment chamber is provided, also coaxial about the central axis, connected by a passage with the mixing chamber. A coring assembly is used to retrieve and insert a fecal sample into the mixing chamber for mixing with a flotation fluid. During centrifugation, heavier fecal components pass radially outwardly to the sediment chamber while the ova collect on the inward surface of the flotation fluid. After centrifugation, more flotation fluid is added, if needed, until a meniscus forms at the top opening. A coverslip is placed over the top opening and the ova float to the surface of the fluid and adhere to the coverslip. The coverslip is removed and the ova detected using standard microscopy procedures. In another aspect, a centrifugal device is provided in which the ova are delivered through centrifugation to a pipette tip for dispensing onto a microscope slide or coverslip. | 2009-10-15 |
20090258412 | REACTOR - Disclosed herein is a reactor, including, a plurality of reaction regions, a plurality of heating elements, each arranged in each of the reaction regions, and cooling elements that cool other regions than reaction regions which are heated by the heating elements, wherein the heating element including a heater and a temperature detecting element and having a detection section configured to detect temperature from the temperature detecting element and a temperature control section configured to control the heater's temperature according to the detected temperature information. | 2009-10-15 |
20090258413 | REACTOR - Disclosed herein is a reactor including, a plurality of reaction regions, and a plurality of heaters, each arranged in each of the reaction regions, wherein the heater including a semiconductor heat generating element and a semiconductor temperature detecting element and being capable of independent temperature control, and the temperature detecting element having a heat conduction region of metal thin film in its surrounding region. | 2009-10-15 |
20090258414 | SYSTEM FOR FLUORESCENCE MONITORING - The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye. | 2009-10-15 |
20090258415 | Fluid Manipulation Assembly - Microfluidic assemblies, systems, and methods are provided for manipulating fluid samples. Assemblies include an elastically deformable cover layer and a less elastically deformable substrate. The methods include deforming the substrate through the cover layer so that when the cover layer rebounds a new communication results in the assembly between the cover layer and the substrate and/or so that a new barrier wall is formed. Systems for carrying out the methods are also provided. | 2009-10-15 |
20090258416 | POLYMER MICELLE COMPLEX INCLUDING NUCLEIC ACID - It is an object of the present invention to provide a polyion complex used as a non-viral gene vector, which achieves sufficiently high gene expression efficiency to a target cell. The polyion complex of the present invention comprises a block copolymer formed by binding polyethylene glycol to polycation via a disulfide group and a nucleic acid. | 2009-10-15 |
20090258417 | Culture Vessel and a Method of Culture - A culture vessel comprising a gas permeable film which has oxygen permeability of P | 2009-10-15 |
20090258418 | Reactive 1,3'-crosslinked carbocyanines and their bioconjugates - Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3′-position, their bioconjugates and their uses are described. 1,3′-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1′-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens. | 2009-10-15 |
20090258419 | METHOD FOR REMOVING PRION PROTEIN - The present invention relates to a method for removing prion PrP | 2009-10-15 |
20090258420 | Altered polypeptides, immunoconjugates thereof, and methods related thereto - The present invention features inter alia altered binding polypeptides having engineered cysteine residues or analogs thereof at a predetermined site within, for example, a constant region domain or a portion thereof. The engineered cysteine residues or analogs thereof provide sites for conjugating effector moieties (e.g. diagnostic or therapeutic agents) that impart novel functionality to the binding polypeptide, preferably without interfering with a desirable property (e.g. an Fc-mediated effector function). The invention includes methods for the rational design of such altered polypeptides, as well as methods for modifying (ie. conjugating) the altered polypeptides with desirable effector moieties. Particular modified binding polypeptides (ie. immunoconjugates) of altered binding polypeptides and methods for utilizing such modified binding polypeptides as protein-based therapeutics are also provided. | 2009-10-15 |
20090258421 | EMBRYONIC STEM CELLS AND NEURAL PROGENITOR CELLS DERIVED THEREFROM - The present invention provides undifferentiated human embryonic stem cells, methods of cultivation and propagation and production of differentiated cells. In particular it relates to the production of human ES cells capable of yielding somatic differentiated cells in vitro, and committed progenitor cells such as neural progenitor cells capable of giving rise to mature somatic cells including neural cells and/or glial cells and uses thereof. The invention also provides methods that generate in vitro and in vivo models of controlled differentiation of ES cells towards the neural lineage. The model, and the cells that are generated along the pathway of neural differentiation may be used for the study of the cellular and molecular biology of human neural development, for the discovery of genes, growth factors, and differentiation factors that play a role in neural differentiation and regeneration, for drug discovery and for the development of screening assays for teratogenic, toxic and neuroprotective effects. | 2009-10-15 |
20090258422 | OPTICALLY DETECTABLE ORGANOPHOSPHONATES - The invention relates to compounds having general formula I | 2009-10-15 |
20090258423 | Cell cycle regulation and differentiation - The present invention provides compositions and methods for regulating neural cell proliferation or differentiation. The present invention also provides methods for selecting for bioactive agents effective in regulating proliferation or differentiation. | 2009-10-15 |
20090258424 | Cellular Delivery of siRNA - The invention provides a method for delivering a nucleic acid to a cell using a targeting molecule that is bound non-covalently to the nucleic acid. Compositions and kits are also provided. | 2009-10-15 |
20090258425 | GENE KNOCK-DOWN BY INTRACELLULAR EXPRESSION OF APTAMERS - Materials and Methods are provided for target validation by gene knock-down with intracellularly expressed aptamers and siRNAs. The aptamers produced by the materials and methods of the invention are useful in target validation for therapeutics development. | 2009-10-15 |