38th week of 2022 patent applcation highlights part 29 |
Patent application number | Title | Published |
20220298493 | INACTIVATION PROCESS FOR VIRUSES - Described herein are methods for inactivation of viruses with high yield and recovery, and compositions produced by such methods. | 2022-09-22 |
20220298494 | ENZYMES WITH RUVC DOMAINS - The present disclosure provides for endonuclease enzymes having distinguishing domain features, as well as methods of using such enzymes or variants thereof. | 2022-09-22 |
20220298495 | NOVEL GENOME EDITING TOOL - The present invention provides a novel gene editing composition comprising at least one fusion protein, which comprises a retrotransposon-encoded protein portion linked to a CRISPR nuclease portion, and an RNA template molecule comprising an insert template sequence. | 2022-09-22 |
20220298496 | PROCESS AND SYSTEM FOR OBTAINING BOTULINUM NEUROTOXIN - Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield | 2022-09-22 |
20220298497 | HIGH EFFICIENCY OXALATE-DEGRADING ENZYMES FOR DEGRADATION OF INSOLUBLE AND SOLUBLE OXALATE - Disclosed herein are oxalate inducing enzymes with pH and thermal stability and methods of using for treatment of oxalate related conditions for in food processing. | 2022-09-22 |
20220298498 | METHOD OF SCREENING CO2-ASSIMILATING MICROORGANISM - The present invention provides a method of producing a microorganism having an ability of assimilating a first factor including: a step of subjecting a microorganism to random mutagenesis; and a step of culturing the microorganism in the presence of a highly concentrated first factor and then selecting the grown microorganism. | 2022-09-22 |
20220298499 | ARTIFICIAL GENE AND METHOD FOR MUTATING GENE - An artificial gene has a | 2022-09-22 |
20220298500 | COMPOSITIONS FOR REGULATING AND SELF-INACTIVATING ENZYME EXPRESSION AND METHODS FOR MODULATING OFF-TARGET ACTIVITY OF ENZYMES - A gene editing nuclease expression cassette is provided which comprises a nucleic acid sequence comprising an nuclease coding sequence which is operably linked to regulatory sequences which direct expression of the nuclease following delivery to a host cell having a sequence to which the nuclease is targeted and at least one nuclease modulating sequence which is selected from the target sequence for the nuclease or a mutated target sequence which is recognized by the nuclease following its expression. A vector is provided comprising the gene editing nuclease expression cassette. Also provided are compositions containing same and methods of use. | 2022-09-22 |
20220298501 | CRISPR-ASSOCIATED MU TRANSPOSASE SYSTEMS - Systems and methods for targeted gene modification, targeted insertion, perturbation of gene transcripts, and nucleic acid editing. Novel nucleic acid targeting systems comprise components of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems and transposable elements. | 2022-09-22 |
20220298502 | NUCLEASE AND NICKASE FUSION PROTEINS FOR INCREASED HOMOLOGOUS RECOMBINATION IN MAMMALIAN CELLS - The present disclosure provides compositions and methods to increase the percentage of edited cells in a cell population when employing nucleic-acid guided editing, as well as automated instruments for performing these methods. | 2022-09-22 |
20220298503 | SELECTIVE DEGRADATION OF PROTEINS - The present disclosure provides methods to identify peptides and small molecule moieties that are able to functionally bridge an interaction between a target protein and an E3 ubiquitin ligase to mediate the degradation of the target protein. Some moieties can degrade specific target variants, but not others. The moieties create a neosubstrate for an E3 ligase of interest. The methods described enable generation of compounds able to selectively degrade specific targets within cells with implications for drug development for pathological conditions. The disclosure also describes the generation of modified peptides using post-translational modification enzymes, such as N-methyltransferases, prolyloligopeptidases, lactamases, hydroxylases, and dehydratases, along with methods of using the same. | 2022-09-22 |
20220298504 | Application of Immobilized Enzymes for Nanopore Library Construction - The present disclosure relates, according to some embodiments, to methods for preparing a library for sequencing. For example, a method may comprise (a) in a coupled reaction, (i) contacting a population of nucleic acid fragments with a tailing enzyme to produce tailed fragments, and (ii) ligating to the tailed fragments a sequencing adapter with a ligase to produce adapter-tagged fragments; and/or separating adapter-tagged fragments from the tailing enzyme and the ligase to produce separated adapter-tagged fragments and, optionally, separated tailing enzyme and/or separated ligase. In some embodiments, a tailing enzyme and/or a ligase used in library preparation may be immobilized enzymes. | 2022-09-22 |
20220298505 | METHOD FOR CONSTRUCTING PACBIO SEQUENCING LIBRARY - Provided in the present invention is a method for constructing a PacBio sequencing library, comprising the following steps: (1) obtaining a target double-stranded DNA; (2) adding a thermostable RNA ligase to respectively connect two ends of the double-stranded DNA to form a closed loop to obtain a dumbbell-shaped DNA library; (3) purifying the dumbbell-shaped DNA library; and (4) binding with sequencing primers and adding a DNA polymerase to obtain a PacBio sequencing library. | 2022-09-22 |
20220298506 | Novel Retinitis Pigmentosa Treatment - An isolated or purified antisense oligomer for modifying pre-mRNA splicing in the CNOT3 gene transcript or part thereof. | 2022-09-22 |
20220298507 | COMPOSITIONS AND METHODS FOR RNA INTERFERENCE - The disclosure provides inhibitory RNA polynucleotides that have partial complementarity to a target gene. The inhibitory RNA polynucleotides have at least one mismatched nucleotide and can be designed to increase or decrease the cleavage rate when loaded onto the RNA-induced silencing complex (RISC). | 2022-09-22 |
20220298508 | GALNAC CLUSTER PHOSPHORAMIDITE AND TARGETED THERAPEUTIC NUCLEOSIDES - Provided herein are oligonucleotide agents comprising one or more therapeutic oligonucleotides such as siRNA and one or more targeting conjugate compounds. In certain embodiments, the conjugate compound comprises one or more N-Acetylgalactosamine as targeting group, branching group and linker group. By incorporating long carbon chains into the GalNAc clusters instead of using multiple amide groups to elongate the chain length, simplification of the synthesis by reducing the number of steps is achieved. | 2022-09-22 |
20220298509 | MULTI-INPUT MIRNA SENSING WITH CONSTITUTIVE ERNS TO REGULATE MULTI-OUTPUT GENE EXPRESSION IN MAMMALIAN CELLS - Provided herein are sequestrons for detecting an miRNA profile indicative of a cell state and expressing an output molecule in cells having such an miRNA profile. Also provided are methods of using sequestrons provided herein. | 2022-09-22 |
20220298510 | COMPOUNDS AND METHODS FOR INHIBITING CANCERS VIA REST INHIBITION - Compositions and methods for diagnosing and treating cancers that have developed from neuronal cells and neuronal cell lines. | 2022-09-22 |
20220298511 | APPLICATION OF COMBINED INHIBITION OF LNCRNA SAMMSON AND BRAF KINASE IN DELAYING ADAPTIVE DRUG-RESISTANCE OF MELANOMA - The present disclosure discloses use of combined inhibition of LncRNA SAMMSON and BRAF kinase for delaying adaptive drug-resistance of melanoma. The present disclosure reveals that the RAF/MEK/ERK kinase can rapidly up-regulate the RNA level of LncRNA SAMMSON in BRAF-mutated melanoma cells. Knocking down the SAMMSON can significantly facilitate the BRAF kinase inhibitor vemurafenib-induced apoptosis and increase the sensitivity of the cells to vemurafenib. | 2022-09-22 |
20220298512 | SIRNA SEQUENCES TARGETING THE EXPRESSION OF HUMAN GENES JAK1 OR JAK3 FOR A THERAPEUTIC USE - A double-stranded (ds) ribonucleic acid (RNA) comprising a sense strand and an antisense strand, wherein: the sense strand comprises the nucleotide sequence SEQ ID NO: 1 and the antisense strand comprises the nucleotide sequence SEQ ID NO: 2; or the sense stand comprises the nucleotide sequence SEQ ID NO: 3 and the antisense strand comprises the nucleotide sequence SEQ ID NO: 4; or the sense strand comprises the nucleotide sequence SEQ ID NO: 5 and the antisense strand comprises the nucleotide sequence SEQ ID NO: 6; or the sense strand comprises the nucleotide sequence SEQ ID NO: 7 and the antisense strand comprises the nucleotide sequence SEQ ID NO: 8. The dsRNA therefrom may be used as a drug. | 2022-09-22 |
20220298513 | METHOD OF IDENTIFYING INTRACELLULAR SECRETORY PROTEIN OR TISSUE-SPECIFIC SECRETORY PROTEIN - The present invention relates to a method of identifying an intracellular secretory protein or tissue-specific secretory protein, by using a proximity labeling system. When the method according to the present invention is used, it is possible to clearly identify an intracellular secretory protein and to dynamically track the spatiotemporal dynamics of a secretory protein secreted from a specific tissue in a living subject such that it can be effectively utilized for the research on endocrine signals between tissues, and particularly, since it can be applied in situ, the scope of application can be further expanded. Therefore, the present invention can be applied to various disease models or tissues to discover new biomarkers and therapeutic target proteins associated with diseases. | 2022-09-22 |
20220298514 | AAV-ZYME AND USE FOR INFUSION REPLACEMENT THERAPY - The invention described herein provides methods and compositions for using recombinant AAV-based viral vectors to express any gene-of-interest (GOI), preferably from muscle and/or liver tissues, for secretion of the polypeptides encoded by the GOI into the bloodstream, thus serving as an alternative treatment regimen for diseases caused by or characterized by lack of functional GOI, or for disease caused by or characterized by a pathological antigen that can be neutralized by a neutralizing peptide encoded by the GOI, such as an immunoglobulin, antibody, or antigen-binding fragment thereof. The method/composition of the invention can be used as an alternative for enzyme replacement therapy (ERT), or for antibody-based therapy. | 2022-09-22 |
20220298515 | ULTRASOUND-BASED THERMOGENETICS FOR IMMUNOTHERAPY - Provided are compositions, products of manufacture, kits and methods for remotely-controlled and non-invasive manipulation of intracellular nucleic acid expression, genetic processes, function and activity in live cells such as T cells in vivo, for activating, adding functions or changing or adding specificities for immune cells, for monitoring physiologic processes, for the correction of pathological processes and for the control of therapeutic outcomes. Provided are ultrasound-based stimulations and a heat-sensitive activation of proteins caused by the activation of polypeptides controlled by heat shock protein promoters to control the production of intracellular nucleic acid and gene expression, for example, for the expression of biological-active proteins such as T cell receptors, for example, chimeric antigen receptors (CARs). Thermogenetic systems as provided herein can be based on ultrasound and/or heat, allowing a deep penetration of stimulation and manipulation in vivo at centimeter-level depth with high spatiotemporal precision. | 2022-09-22 |
20220298516 | COMPOSITIONS AND METHODS FOR DELIVERY OF NUCLEIC ACIDS - The present disclosure relates to methods and compositions for modulating protein expression. In particular, the invention features methods and compositions for increasing protein expression in a cell by delivering to the cell a composition including an mRNA encoding a polypeptide and one or more oligonucleotides, wherein each of the one or more oligonucleotides includes a region of linked nucleotides complimentary to a portion of the sequence of the mRNA. The methods and compositions described herein may be used to modulate gene expression (e.g., increase gene expression), to increase the stability of the mRNA, to decrease the immunogenicity of the mRNA, to enable selective expression (e.g., in a target cell or tissue) of the mRNA, and/or to enable the delivery of two or more mRNAs in a stoichiometric ratio. | 2022-09-22 |
20220298517 | COUNTER-SELECTION BY INHIBITION OF CONDITIONALLY ESSENTIAL GENES - The present invention relates to a method for counter-selection by inhibition of conditionally essential genes. | 2022-09-22 |
20220298518 | PLANT MODIFICATION METHOD USING AXILLARY BUD MERISTEM - A method for transforming a plant includes exposing a shoot apex of an axillary bud of a plant body, and introducing, into the shoot apex, a microparticle coated with at least one kind of nucleic acid. | 2022-09-22 |
20220298519 | POD SHATTER TOLERANCE IN BRASSICA PLANTS - This disclosure provides methods and compositions for identifying | 2022-09-22 |
20220298520 | COMPOSITIONS AND METHODS FOR IMPROVING POD SHATTER TOLERANCE IN CANOLA - Genome edited plants, plant cells, seeds and plant parts of | 2022-09-22 |
20220298521 | METHOD FOR BIOTRANSFORMATION OF TRICHOTHECENES - The present invention relates to a novel method for detoxification of trichothecene contaminated material. More specifically the present invention pertains to a method for biotransformation of a trichothecene by contacting material contaminated with trichothecenes with an exogenous non-animal glutathione-S-transferase (GST) having substrate specificity for the epoxide ring of the trichothecene. The invention further relates to recombinant GSTs and transgenic plants and animals expressing said GSTs. | 2022-09-22 |
20220298522 | METHODS OF CONTROLLING CANNABINOID SYNTHESIS IN PLANTS OR CELLS AND PLANTS AND CELLS PRODUCED THEREBY - A method of controlling cannabinoid synthesis in a cell or plant or plant part comprising same is provided. The method comprising modulating expression in the cell of at least one polypeptide comprising an amino acid sequence at least 95% identical to SEQ ID NO: 1-15 and 18-86, the polypeptide modulating cannabinoid synthesis, thereby controlling cannabinoid synthesis in the cell. Also provided are methods of producing cannabinoids and selecting plants producing cannabinoids of interest. | 2022-09-22 |
20220298523 | GENETICALLY MODIFIED PLANTS AND METHODS OF MAKING THE SAME - Provided herein are compositions comprising genetically modified cells, organisms, or plants described herein or extracts and products thereof and methods for making and using the same. Also provided are therapeutics derived from genetically modified cells, organisms, or plants described herein or extracts and products thereof for use in preventing, treating, or stabilizing disease and conditions. | 2022-09-22 |
20220298524 | FRUIT-BEARING PLANT EXHIBITING HIGH TEMPERATURE RESISTANCE, HIGH YIELD, AND PARTHENOCARPY - The present application provides: a fruit-bearing plant exhibiting parthenocarpy or a potion thereof including a mutation in a Solyc10g038170 gene or ortholog thereof, the mutation causing deficiency or reduction in expression of a protein encoded by the Solyc10g038170 gene or ortholog thereof; a method for producing a parthenocarpic fruit-bearing plant or a potion thereof; and a method for selecting a parthenocarpic fruit-bearing plant or a potion thereof. | 2022-09-22 |
20220298525 | MUTATED PROTOPORPHYRINOGEN IX OXIDASE (PPX) GENES - Provided are compositions and methods relating to gene and/or protein mutations in transgenic or non-transgenic plants. In certain embodiments, the disclosure relates to mutations in the protoporphyrinogen IX (PPX) gene. In some embodiments the disclosure relates to plants that are herbicide resistant. | 2022-09-22 |
20220298526 | STSCI PROTEIN FOR CHANGING SELF-INCOMPATIBILITY OF DIPLOID POTATO MATERIALS - Provided is a StSCI protein for changing the self-incompatibility of diploid potato materials, wherein the amino acid sequence of the StSCI protein includes or consists of the following sequence: 1) the amino acid sequence represented by SEQ ID NO: 1; or 2) a functional homologous sequence having at least 95% sequence identity with the amino acid sequence represented by SEQ ID NO: 1; or 3) a protein in which one or more (e.g., 1-10) amino acids are added, deleted, or replaced in the amino acid sequence represented by SEQ ID NO: 1 and has the activity of inhibiting self-incompatibility. The advantage of the application is that the StSCI protein may inhibit the cytotoxicity of multiple types of S-RNase, which is hereditary and fundamentally overcomes the defect of self-incompatibility of diploid potatoes, thereby facilitating to realize the cultivation of a high-generation homozygous inbred line of diploid potatoes. | 2022-09-22 |
20220298527 | COMPOSITIONS AND METHODS FOR IMPROVING CROP YIELDS THROUGH TRAIT STACKING - The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits and yield relative to a control plant, such as increased ear area, increased ear volume, increased single kernel weight, increased ear fresh weight, increased ear diameter, increased ear length, increased yield, increased kernels per ear, decreased ear tip void, and decreased ear void. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more CONSTANS (CO) or CONSTANS-like (COL) polypeptides and a transgene encoding one or more MADS-box polypeptides, and can also have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants. | 2022-09-22 |
20220298528 | VIRAL PARTICLES FOR USE IN TREATING SYNUCLEINOPATHIES SUCH AS PARKINSON'S DISEASES BY GENE THERAPY - The present disclosure relates to viral particles for use in treating synucleinopathies, particularly sporadic Parkinson Diseases by gene therapy. More specifically, the present invention relates to a viral particle for use in treating synucleinopathy by gene therapy in a subject in need thereof, said viral particle comprising a nucleic acid construct including a transgene encoding a glucocerebrosidase. | 2022-09-22 |
20220298529 | COMPOUND INTRODUCTION APPARATUS AND COMPOUND INTRODUCTION METHOD - Provided is a compound introduction apparatus including: a processing chamber; a first heater that introduces an introduction-target compound into a cell inside the processing chamber; a supply flow channel for supplying a cell suspension containing the cell and the introduction-target compound to the processing chamber; a discharge flow channel for discharging the cell suspension from the processing chamber; a second heater that prevents backflow of the cell suspension from the processing chamber into the supply flow channel; and a third heater that pressurizes the discharge flow channel. | 2022-09-22 |
20220298530 | Optimal Chromosomal Insertion Loci - The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention relates to a method to determine the expression stability of a heterologous gene at a chromosomal location in a cell undergoing burden and to produce mutated cells or organisms transformed with a heterologous gene at a chromosomal location, wherein the expression of said heterologous gene is not influenced by a burden or wherein the expression of said heterologous gene is reduced by a burden. The present invention describes methods to locate interesting chromosomal knock-in locations in a cell. Such engineered cells and organisms are applied for the production of bioproducts, such as but not limited to carbohydrates, lipids, proteins, organic acids, amino acids, alcohols, antibiotics and peptides. Preferably, the invention is applied in the technical field of fermentation of metabolically engineered microorganisms. | 2022-09-22 |
20220298531 | APPARATUS AND PROCESS FOR TREATING BIOMASS FOR ON-SITE PRODUCTION OF CELLULOLYTIC ENZYMES AND METHOD OF USING THE ENZYMES TO MANUFACTURE FUELS AND CHEMICALS - An integrated process and corresponding apparatus that produces a relatively clean, delignified cellulose product from lignocellulosic biomass. The method includes treating a portion of the delignified cellulose itself as a substrate to produce on-site cellulolytic enzymes, including further treating the remaining delignified cellulose with the resulting cellulolytic enzymes for in situ enzymatic hydrolysis. The process and apparatus are useful to produce fermentable sugars for cost-effective manufacturing of fermentable sugars, fuels, bioproducts and chemicals. | 2022-09-22 |
20220298532 | METHOD FOR THE PRODUCTION OF ETHANOL FROM CORN FIBRES - Method for the production of ethanol from corn fibres. Invention relates to a process for the preparation of ethanol from a corn fibre containing feedstock by using a set of process steps specifically identified for the treatment of the said feedstock. It particularly relates to the use of a soaking followed by mix acid and enzyme treatment that release fermentable C5 and C6 sugars from said feedstock. It further relates to using a recombinant yeast to convert both C5 and C6 sugars to ethanol. | 2022-09-22 |
20220298533 | BIOSYNTHETIC METHODS FOR THE MODIFICATION OF CANNABINOIDS - Provided is a method of modifying a first cannabinoid into a second cannabinoid or a non-cannabinoid. The method comprises combining the first cannabinoid with an enzyme that can modify the first cannabinoid into the second cannabinoid or non-cannabinoid under conditions where the first cannabinoid is modified into the second cannabinoid or non-cannabinoid. Also provided is a non-naturally occurring enzyme that can modify a first cannabinoid into a second cannabinoid or a non-cannabinoid. A nucleic acid encoding that enzyme is additionally provided. Further provided is a non-naturally occurring nucleic acid that encodes an enzyme having the enzymatic activity of the above non-naturally occurring enzyme. An expression cassette comprising that nucleic acid is additionally provided. A cell comprising the above expression cassette is further provided. Also provided is a plant expression cassette comprising the above-identified nucleic acid. | 2022-09-22 |
20220298534 | METHOD FOR INCREASING THE PRODUCTION OF SMALL MOLECULES IN SUBMERGED CORYNEBACTERIUM CULTURE - Described are methods for increasing the production of small molecules in a submerged | 2022-09-22 |
20220298535 | METHOD FOR THE IN VIVO SYNTHESIS OF 4-HYDROXYMETHYLFURFURAL AND DERIVATIVES THEREOF - The present disclosure provides recombinant microorganisms and methods for the production of 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA from a carbon source. The method provides for engineered microorganisms that express endogenous and/or exogenous nucleic acid molecules that catalyze the conversion of a carbon source into 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. The disclosure further provides methods of producing polymers derived from 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. | 2022-09-22 |
20220298536 | IMPROVED OLIGOSACCHARIDE PRODUCTION IN YEAST - Provided herein are genetically modified yeast cells capable of producing one or more human milk oligosaccharides (HMOs) and methods of making such cells. The yeast cells are engineered to comprise a heterologous nucleic acid encoding a transporter protein and one or more heterologous nucleic acids that encode enzymes of a HMO biosynthetic pathway. Also provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering HMOs generated by the yeast cells. | 2022-09-22 |
20220298537 | Method and System for Selectively Harvesting Products from Plant Cells in Culture - A method of recovering a product-of-interest from a plant or algal cell and a kit relating to the same are provided. The method of recovering a product-of-interest from a plant or algal cell includes (a) culturing a plant or algal cell that produces a product-of-interest in a culture medium; (b) adding functionalized nanoparticles to the culture medium, whereby the nanoparticles enter the plant or algal cell, bind the product-of-interest, and are extruded into the culture medium; (c) recovering the extruded nanoparticles from the culture medium; and (d) separating the product-of-interest from the extruded nanoparticles. The kit includes a plant or algal cell that produces a product-of-interest when in culture and a functionalized nanoparticle. Also provided is a method of separating plant or algal cells. | 2022-09-22 |
20220298538 | KINASE ACTIVITY SIGNATURES FOR PREDICTING THE RESPONSE OF NON-SMALL-CELL LUNG CARCINOMA PATIENTS TO A PD-1 OR PD-L1 IMMUNE CHECKPOINT INHIBITOR - The present invention relates to a method for determining or predicting the response of a patient diagnosed with non-small-cell lung carcinoma a PD-1 or PD-L1 immune checkpoint inhibitor. The present invention also aims to provide methods and devices for predicting the response of patients diagnosed with non-small-cell lung carcinoma to PD-1 or PD-L1 immune checkpoint inhibitors. More specifically, the present invention provides methods which measure kinase-activity and profiles and inhibitions thereof by drugs in blood samples of said patients. | 2022-09-22 |
20220298539 | METHODS AND KITS FOR IDENTIFYING THROMBUS ORIGIN AND OPTIMIZING POST-ISCHEMIC EVENT TREATMENT - Provided herein are method and kits for identifying thrombus origin based on temporal thrombin activity assay, and use thereof for optimizing post-ischemic event treatment, and further preventing recurrence of secondary ischemic events. | 2022-09-22 |
20220298540 | METHOD AND REAGENTS FOR DETECTING LUCIFERASE ACTIVITY - The bioluminescent system components are commonly used reagents for a variety of analyzes, including diagnostic systems, quality control systems, drug testing systems, etc. This group of inventions discloses the components of the bioluminescent system | 2022-09-22 |
20220298541 | ASSAY FOR DETERMINING TARGET ENGAGEMENT IN REAL-TIME IN LIVING SYSTEMS - The present disclosure relates to an assay for determining drug-target engagement in real-time. Specifically, the present disclosure provides a method for determining drug candidate-target interaction in real-time in living systems using Activity-based Reporter Gene Technology-Bioluminescence Resonance Energy Transfer (AbRGT-BRET) based assay. The said assay is useful for High Throughput Screening of compounds to a specific target. | 2022-09-22 |
20220298542 | MULTIPLEXED PROFILING OF RNA AND DNA MODIFICATIONS - Provided herein are compositions and methods for the multiplexed profiling of RNA and DNA modifications across transcriptomes and genomes, respectively. The methods combine molecular recognition of non-canonical features (e.g., base modifications, backbone modifications, lesions, and/or structural elements) of a target nucleic acid with a step of writing the information from this recognition event into the neighboring genetic sequence of the target nucleic acid using a barcode. The resultant barcoded nucleic acids are then converted into sequencing libraries and read by DNA/RNA sequencing methods. This step reveals the sequence of the barcode, which is correlated with the non-canonical feature in the target nucleic acid(s). The high throughput profiling methods described herein allow for localization of one or more modifications in a target nucleic acid. The methods also allow for identification of the nature and location of several or all DNA/RNA modifications in parallel. | 2022-09-22 |
20220298543 | MULTIPLEXED PROFILING OF RNA AND DNA MODIFICATIONS - Provided herein are compositions and methods for the multiplexed profiling of RNA and DNA modifications across transcriptomes and genomes, respectively. The methods combine molecular recognition of non-canonical features (e.g., base modifications, backbone modifications, lesions, and/or structural elements) of a target nucleic acid with a step of writing the information from this recognition event into the neighboring genetic sequence of the target nucleic acid using a barcode. The resultant barcoded nucleic acids are then converted into sequencing libraries and read by DNA/RNA sequencing methods. This step reveals the sequence of the barcode, which is correlated with the non-canonical feature in the target nucleic acid(s). The high throughput profiling methods described herein allow for localization of one or more modifications in a target nucleic acid. The methods also allow for identification of the nature and location of several or all DNA/RNA modifications in parallel. | 2022-09-22 |
20220298544 | METHOD FOR ANALYZING TARGET PARTICLE, ANALYTICAL REAGENT, AND ANALYZER - According to one embodiment, a method includes dispensing the specimen into first | 2022-09-22 |
20220298545 | Methods and Compositions for Tracking Nucleic Acid Fragment Origin for Nucleic Acid Sequencing - The present disclosure provides methods and compositions for tracking nucleic acid fragment origin by target-specific barcode tagging when original nucleic acid targets break into small fragments. Nucleic acid targets are captured in vitro on a solid support with clonally localized nucleic acid barcode templates. Many nucleic acid targets can be processed simultaneously in a massively parallel fashion without partition. These nucleic acid target tracking methods can be used for a variety of applications in both whole genome sequencing and targeted sequencing in order to accurately identify genomic variants, haplotype phasing and assembly, for example. | 2022-09-22 |
20220298546 | NON-THERMAL CYCLING FOR POLYMERASE CHAIN REACTION - Techniques, systems, and devices are disclosed for non-thermal cycling of polymerase chain reaction (PCR). In one aspect, a method for cycling PCR includes receiving an electrolytic fluid including ions, primers, polymerase enzymes, nucleotides, and a double-stranded nucleic acid in a fluid chamber having a first electrode and a second electrode, applying an electric field across the first and the second electrodes to generate a first pH level of the electrolytic fluid to denature the double-stranded nucleic acid to at least partial single strands, and applying a second electric field across the first and second electrodes to produce a second pH level of the electrolytic fluid, in which the second pH level enables binding of a polymerase enzyme and a primer with a corresponding segment of the single strands. | 2022-09-22 |
20220298547 | Multiplex Viral Pathogen Analysis and Uses Thereof - Provided herein are methods for isolating, detecting and analyzing a virus in a sample. Generally, the virus is isolated via centrifuging, incubating, aggregating, and lysing the sample to obtain a crude neutralized lysate. The crude neutralized lysate is analyzed and the virus(es) are detected by performing one of a first polymerase chain reaction (PCR) amplification followed by a second PCR amplification, by an isothermal amplification or by the reverse transcription reaction and the first polymerase chain reaction amplification together utilizing fluorescently labeled primer pairs and hybridizing the resultant fluorescent labeled amplicons to complementary nucleic acid probes. | 2022-09-22 |
20220298548 | COMPOSITIONS, METHODS AND KITS FOR DETECTING TREPONEMA PALLIDUM - Presented are oligonucleotide hybridization probes, primer sets, methods, reaction mixtures, and kits for detecting nucleic acids of | 2022-09-22 |
20220298549 | METHOD FOR IDENTIFYING WHETHER A DIPLOID POTATO IS SELF-COMPATIBLE - The present application relates to the technical field of genetic breeding, and provides a method for identifying whether a diploid potato is self-compatible. The method relates to identifying whether a StSCI gene in the diploid potato is transcribed and expressed. Also disclosed is a method for identifying whether a StSCI gene is expressed by using molecular marker, and a method of screening for the molecular marker, which includes: obtaining the genome sequence information of parental materials, screening for difference sites of the parental materials, screening for the molecular marker, and identifying whether the screened molecular marker are usable. As for the identification of the self-compatibility of a diploid potato by using the screened molecular marker, the identification workload is small, a lot of time is saved, and the identification result is not affected by the environment, and it is accurate and reliable. | 2022-09-22 |
20220298550 | CHEMICAL SENSOR DEVICE AND METHOD FOR MANUFACTURING THE SAME - A chemical sensor device includes a sensor element; an oligopeptide mounted on a surface of the sensor element, the oligopeptide containing a first peptide sequence forming a β-sheet structure and a cysteine residue at a position different from the first peptide sequence; and a probe that is bonded to the cysteine residue and binds to or reacts with a specific substance. | 2022-09-22 |
20220298551 | COMPOSITIONS AND METHODS FOR NUCLEIC ACID ANALYSIS - Provided herein are methods, systems, and compositions for determining a base in a polynucleotide. In various aspects, the methods, systems, and compositions presented herein are useful for performing 4-base, 5-base, or 6-base sequencing of polynucleotide molecules, for example, from liquid biopsy samples or wherein the base is a low frequency mutation. | 2022-09-22 |
20220298552 | METHOD FOR IDENTIFYING HIGH-AFFINITY COMPLEXES MADE OF TWO LIGANDS AND ONE RECEPTOR, DEVICE FOR IMPLEMENTING THE METHOD AND SELF-ASSEMBLING CHEMICAL LIBRARY FOR USE IN THE METHOD - The present invention relates to a method for the sensitive identification of high-affinity complexes made of two ligands ( | 2022-09-22 |
20220298553 | METHOD AND SYSTEM EMPLOYING DISTINGUISHABLE POLYMERASES FOR DETECTING TERNARY COMPLEXES AND IDENTIFYING COGNATE NUCLEOTIDES - Method of identifying a cognate nucleotide (i.e., the “next correct nucleotide”) for a primed template nucleic acid molecule. In some embodiments, an ordered or random array of primed target nucleic acids characterized by different cognate nucleotides can be evaluated using a single imaging step to identify different cognate nucleotides for a collection of different primed template nucleic acid molecules. An optional incorporation step can follow the identifying step. A polymerase different from the ones used in the binding and examination steps can be used to incorporate a nucleotide, such as a reversible terminator nucleotide, preliminary to identification of the next cognate nucleotide. | 2022-09-22 |
20220298554 | METHOD AND KIT FOR MEASURING OF ANALYTES IN BI-COMPONENT SYSTEMS AND USES THEREOF - A method and a kit are for measuring parameters of an analyte using bi-component detection methods. The method includes measurements of dilutions of the analyte with an extended measurable concentration range of measurements. The determination of dissociation constants of components of bi-component systems is made using the measurements of dilutions of the analyte. Quantitation can be carried out in a highly parallel manner. | 2022-09-22 |
20220298555 | DETECTION OF TARGET NUCLEIC ACID BY SOLID-PHASE MOLOGRAPHY - The present invention describes methods for performing real-time PCR for detection and quantitation of target nucleic acids using tagged oligonucleotide probes and solid-phase molography. | 2022-09-22 |
20220298556 | PIRNA-54265 DETECTION KIT USED FOR EARLY SCREENING, DIAGNOSIS, CURATIVE EFFICACY MONITORING AND PROGNOSTIC EVALUATION OF COLORECTAL CANCER - The present invention relates to a piRNA-54265 detection kit used for early screening, diagnosis, efficacy monitoring and/or prognostic evaluation of colorectal cancer. The detection kit includes a primers combination, including a primer pair and a probe for specifically detecting piRNA-54265; the primer pair is a piRNA-54265 stem-loop PCR primer pair, or a piRNA-54265 PolyA tailed PCR primer pair; the primer pair includes a forward primer and a reverse primer. | 2022-09-22 |
20220298557 | METHOD FOR DESIGNING A PROBE COMBINATION - Methods for designing and producing a fluorescent-labeled detection probe and a competitive probe combination are provided to improve detection by reducing noise. A method for designing the fluorescent-labeled detection probe and a competitive probe combination includes, for example, determining the base length and the base sequence of each of the fluorescent-labeled detection probe and the competitive probe. The determining can include experimentally determining the amount to be added to the nucleic acid sample of each of the fluorescent-labeled detection probe and the competitive probe. The methods provide a functional result of a first order derivative curve for the control target reaction sample having a substantial peak (maximum value), but a first order derivative curve for the control non-target reaction sample not having a substantial peak, the functional result improving detection by reducing noise. | 2022-09-22 |
20220298558 | KIT FOR DETECTING A MUTATED TARGET BASE SEQUENCE - Kits for detecting a mutated target base sequence are provided. For example, the kits can be for detecting a target base sequence (A) containing a nucleotide with a mutated base from a nucleic acid sample. The kits contain a fluorescent-labeled detection probe and a competitive probe combination that is to improve detection by reducing noise. The probe combination is produced using a process that includes, for example, determining the base length and the base sequence of each of the fluorescent-labeled detection probe and the competitive probe. The determining can include experimentally determining the amount to be added to the nucleic acid sample of each of the fluorescent-labeled detection probe and the competitive probe. The kits provide a functional result of a first order derivative curve for the control target reaction sample having a substantial peak (maximum value), but a first order derivative curve for the control non-target reaction sample not having a substantial peak, the functional result improving detection by reducing noise. | 2022-09-22 |
20220298559 | METHODS AND SYSTEMS FOR SAMPLE PROCESSING OR ANALYSIS - The present disclosure provides methods and systems for detecting nucleic acid sequences in a biological sample having a three-dimensional matrix. The present disclosure also provides methods and systems for processing a biological sample for use in nucleic acid sequence detection. | 2022-09-22 |
20220298560 | SPATIALLY DISTINGUISHED, MULTIPLEX NUCLEIC ACID ANALYSIS OF BIOLOGICAL SPECIMENS - A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen. | 2022-09-22 |
20220298561 | SYSTEMS AND METHODS FOR SINGLE MOLECULE QUANTIFICATION - A method for quantifying labels on a substrate is performed by an electronic device with one or more processors and memory. The method includes obtaining digital data corresponding to a multi-dimensional measurement over the substrate; identifying a first set of sub-portions of the digital data; and, for a respective sub-portion of the first set of sub-portions of the digital data: increasing a quantity of labels, and subtracting a reference signal distribution from the respective sub-portion to obtain subtracted sub-portion data. The method also includes obtaining subtracted digital data. The subtracted digital data includes the subtracted sub-portion data for the respective sub-portion. The method further includes identifying a second set of one or more sub-portions of the subtracted digital data; and, for a respective sub-portion of the second set of one or more sub-portions of the subtracted digital data, increasing a quantity of labels. | 2022-09-22 |
20220298562 | METHODS AND SYSTEMS FOR SENSITIVE AND MULTIPLEXED ANALYSIS OF BIOLOGICAL SAMPLES USING HIGH-PERFORMANCE CLEAVABLE, DETECTABLY-LABELED TYRAMIDE - Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, that allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal. | 2022-09-22 |
20220298563 | MATERIALS AND METHODS FOR SERIAL MULTIPLEXED DETECTION OF RNA IN CELLS AND TISSUES - This disclosure describes materials and methods for effectively performing serial multiplexed FISH analysis. | 2022-09-22 |
20220298564 | HIGH SENSITIVITY DETECTION OF MICROSATELLITE LOCI BY BLOCKER DISPLACEMENT AMPLIFICATION WITH MULTIPLE BLOCKERS AND ITS USE IN MICROSATELLITE INSTABILITY DETECTION - This invention describes methods and compositions for selectively enriching unstable variants at a particular microsatellite locus. | 2022-09-22 |
20220298565 | Method Of Determining PIK3CA Mutational Status In A Sample - An ultra-sensitive, specific methodology for detecting PIK3CA mutations in biological samples of cancer patients, comprises a combination of allele-specific, asymmetric rapid PCR and melting analysis in a DNA sample from Circulating Tumor Cells, cell-free DNA in plasma/serum, or Formalin-Fixed Paraffin-Embedded tissues. Using the allele-specific primers for hotspot mutations in exons 9 and 20 (E545K and H1047R), detection can enhance amplification of mutant PIK3CA allele sequence, whereas presence of corresponding competitive blocking unlabeled probes for each exon can avoid non-specific amplification of wild-type PIK3CA sequence increasing the sensitivity and the specificity of method. The mutational detection is completed with melting curve analysis of the unlabeled probe and DNA template of the mutant PIK3CA sequence. Evaluation of PIK3CA mutational status on CTC in peripheral blood and cfDNA in plasma/serum of patients has potential for clinical applications and therapeutic interventions, since presence of PIK3CA mutations is associated with response to molecular targeted therapies. | 2022-09-22 |
20220298566 | METHODS FOR ACCURATE PARALLEL QUANTIFICATION OF NUCLEIC ACIDS IN DILUTE OR NON-PURIFIED SAMPLES - The present invention disclosure relates to a next generation DNA sequencing method and use for accurate and massively parallel quantification of one or more nucleic acid targets, for example in large volumes of unpurified sample material. More particularly, the invention is related to a method and a kit comprising probes for detecting and quantifying genetic targets in complex samples. The invention includes one or more target-specific nucleic acid probes per genetic target and a bridge oligo or bridge oligo complex. | 2022-09-22 |
20220298567 | METHOD FOR CONTROLLING BASE SEQUENCE DETERMINATION, BASE SEQUENCE DETERMINATION SYSTEM AND CONTROL DEVICE - The present disclosure discloses a method for controlling a base sequence determination, a base sequence determination system, and a control device. The base sequence determination system includes a fluid device and an optical device, a reaction device includes a first component and a second component, and a repeated executable unit included in the base sequence determination is defined as: a second biochemical reaction—a first biochemical reaction—photographing. The method includes, after initiation steps are completed, using the fluid device to perform the second biochemical reaction and the first biochemical reaction of the sample on the first component, while using the optical device to photograph the sample on the second component. The initial steps include: a. using the fluid device to perform the first biochemical reaction of the sample on the first component, b. using the optical device to photograph the sample on the first component after the first biochemical reaction, and c. using the fluid device to perform the first biochemical reaction of the sample on the second component. The above-mentioned method can improve the efficiency of base sequence determination. | 2022-09-22 |
20220298568 | SURFACE STRUCTURING WITH COLLOIDAL ASSEMBLY - Disclosed herein include methods of specifying sites (e.g., sites for colony formation) on a surface (e.g., a planar surface) and generating a flow cell having the sites specified on a surface. Also disclosed are methods of performing sequencing (e.g., sequencing-by-synthesis and sequencing-by-binding) using the flow cell generated and processing (e.g., aligning, orienting, sorting, and assessing quality) images of the flow cell captured during sequencing. | 2022-09-22 |
20220298569 | HIGHLY SENSITIVE METHODS FOR ACCURATE PARALLEL QUANTIFICATION OF NUCLEIC ACIDS - The present disclosure relates to a next generation DNA sequencing method and use for accurate and massively parallel quantification of one or more nucleic acid targets, for example in large volumes of unpurified sample material. More particularly, the present disclosure is related to a method and a kit comprising probes for detecting and quantifying genetic targets in complex samples. The disclosed embodiments includes one or more target-specific nucleic acid probes per genetic target (left probe and right probe) and a bridge oligo or bridge oligo complex. | 2022-09-22 |
20220298570 | OPTIMIZED NUCLEIC ACID PROBES FOR ANALYTE DETECTION - The present disclosure relates to optimized nucleic acid probes, nucleic acid chips, and assays and methods for detection of an analyte of interest in a sample, for example, an allergen in a food sample. | 2022-09-22 |
20220298571 | SUPER HLA TYPING METHOD AND KIT THEREOF - The present invention method for super Human Leukocyte Antigen (HLA) Typing, gene sequencing and analysis comprising: (a) designing and synthesizing the set of forward and reverse primers for full length amplification of HLA gene class I (HLA-A, B, C) and class II (HLA-DQBI, DRBI, DPBI)selected from group consisting of SEQ ID 1 to 24; (b) amplification of the test sample HLA gene by using the set of primer synthesized at step (a) to obtain PCR amplicon; (c) separation of the PCR amplicon obtained at step (b); (d) sequencing of the separated amplicon of step (c); and (e) the sequences thus generated analyzed by matching with HLA gene from 5′ UTR to 3′ UTR. | 2022-09-22 |
20220298572 | COMPOSITIONS AND METHODS FOR DISCRIMINATING BACTERIAL AND VIRAL INFECTIONS IN A HUMAN SUBJECT - The present disclosure is directed to methods and kits for discriminating between a bacterial infection and viral infection in a human subject. More specifically, the methods can comprise detecting the expression level of a combination of ABL1, IRF9, ITGAM, LY6E, PSTPIP2 and RUNX1 in biological samples from the human subject and determining whether the human subject has a bacterial or viral infection based on those expression levels. | 2022-09-22 |
20220298573 | DETERMINATION OF AN INFECTION PROFILE IN AN INDIVIDUAL AND USES THEREOF - The present disclosure, in some aspects, is directed to methods of determining an infection profile of an individual, wherein the infection profile is based on a host response profile and a pathogen profile of the individual. In other aspects, the present disclosure is directed to uses (e.g., methods of treatment, treatment selection, patient selection), kits, and compositions of the methods described herein. | 2022-09-22 |
20220298574 | BLOOD BIOMARKERS FOR APPENDICITIS AND DIAGNOSTICS METHODS USING BIOMARKERS - The invention relates to methods and kits for diagnosing and/or treating appendicitis in a subject, comprising performing one or more assays configured to detect one or more biomarkers on a body fluid sample obtained from the subject to provide one or more assay result(s) and correlating the assay result(s) to the occurrence or nonoccurrence of appendicitis in the subject or likelihood of the future outcome to the subject. | 2022-09-22 |
20220298575 | Immunocompetence Assessment by Adaptive Immune Receptor Diversity and Clonality Characterization - Disclosed are methods for determining the immunological status of the adaptive immune system of a subject by identifying and quantifying rearranged DNA (and/or subsequently transcribed RNA) sequences encoding T cell receptor (TCR) and/or immunoglobulin (IG) polypeptides, in a lymphoid DNA-containing sample from the subject. TCR and/or IG sequence diversity and sequence distribution permit immunocompetence and immune repertoire assessment and reflect the degree of T cell or B cell clonality and clonal expansion in the sample. Methods for stratifying patient populations on the basis of immunocompetence including likelihood of responding to immunotherapy are also described. | 2022-09-22 |
20220298576 | METHODS AND COMPOSITIONS FOR PROGNOSIS AND TREATMENT OF CANCERS - The invention provides methods of using expression levels of one or more immune cell gene signatures and/or combinations of immune cell gene signatures as selection criteria for selecting a patient having cancer for treatment with an immunotherapy. The invention further provides methods for selecting a patient having cancer who may benefit from a particular immunotherapy, such as an activating immunotherapy or a suppressing immunotherapy and administering to the patient the activating immunotherapy or suppressing immunotherapy to treat the cancer. | 2022-09-22 |
20220298577 | METHYLATION-BASED MODIFIED TUMOR MARKER STAMP-EP8 AND APPLICATION THEREOF - Provided are an isolated polynucleotide and a use thereof, wherein the isolated polynucleotide is the polynucleotide shown in SEQ ID NO:1 or a fragment or complementary nucleic acid thereof at a modified CpG site. Further provided are the use of the polynucleotide for preparing a tumor detection reagent or a kit, a preparation method, a reagent and a use thereof, a kit, and a method for the in-vitro detection of the methylation profile of a sample. | 2022-09-22 |
20220298578 | DETECTION OF RELB ACTIVATION FOR PREDICTING A PROGNOSTIC IN B-CELL LYMPHOMA - The invention relates to a method for predicting the prognosis of a patient suffering from a B-cell lymphoma, through the detection of the status of activation of the RelB protein, in a biological sample of said patient. The inventors indeed identified an associated gene expression signature in the biological sample. Some genes from said signature are over-expressed and other are under-expressed and allow detecting RelB activation and predicting a worse prognosis in B-cell lymphoma. | 2022-09-22 |
20220298579 | COMPOSITIONS AND METHODS FOR PREDICTING SUSCEPTIBILITY OF CANCERS TO SYNTHETIC-LETHAL THERAPIES - STAT3 (Signal Transducer and Activator of Transcription), which is overactive in two-thirds of human cancers, is shown herein to inhibit HR repair. This newly identified linkage of STAT3 to HR impairment forecasts that many more cancers, beyond just breast and ovarian cancers, are likely to be susceptible to PARP inhibitors and other synthetic lethal therapies. Also disclosed herein is a gene signature that can predict which cancers are likely to respond to therapy with PARP inhibitors and other synthetic lethal therapies. | 2022-09-22 |
20220298580 | IMMUNE SIGNATURES PREDICTIVE OF RESPONSE TO PD-1 BLOCKADE IN RICHTER'S TRANSFORMATION - The present invention relates to methods for identifying Richter's transformation patients who benefit from PD-1 blockade. | 2022-09-22 |
20220298581 | COMPOSITIONS AND METHODS OF USING HisGTG TRANSFER RNAS (tRNAs) - The present invention includes a method for analyzing tRNA | 2022-09-22 |
20220298582 | METHODS FOR DETECTING SIGNATURES OF DISEASE OR CONDITIONS IN BODILY FLUIDS - This invention provides methods of using cell free bodily fluid and blood cells in the diagnosis, prognosis, or monitoring of diseases or conditions. The invention also relates to methods of using cell free bodily fluid and blood cells to identify markers of diseases or conditions. | 2022-09-22 |
20220298583 | METHODS FOR DETECTING SIGNATURES OF DISEASE OR CONDITIONS IN BODILY FLUIDS - This invention provides methods of using cell free bodily fluid and blood cells in the diagnosis, prognosis, or monitoring of diseases or conditions. The invention also relates to methods of using cell free bodily fluid and blood cells to identify markers of diseases or conditions. | 2022-09-22 |
20220298584 | Identification of Host RNA Biomarkers of Infection - The inventive technology includes novel systems, method and compositions for the identification and classification of host-derived RNA biomarkers produced in response to an infection. | 2022-09-22 |
20220298585 | DETECTION OF INFECTIOUS AGENTS FROM ENVIRONMENTAL AIR DUST - Embodiments of the present disclosure are directed to systems and methods for collection and analysis of environmental air dust (EAD) within an individually ventilated cage rack (IVR) environment for detecting pathogens. The method includes collection of an EAD sample by a collection media, isolation of a plurality of nucleic acids (e.g., RNA and/or DNA) representative of one or more infectious agents from the EAD sample, optional reverse transcription of RNA to cDNA if the isolated nucleic acids contain RNA, amplification of the cDNA and/or DNA (e.g., by polymerase chain reaction (PCR)), and assay interpretation. Optionally, the EAD sample may be analyzed with one or more other sample types (e.g., fecal pellets, oral swabs, body swabs, tissue, etc.) to improve detection of low-copy organisms. | 2022-09-22 |
20220298586 | PRIMER PAIR FOR DETECTING PNEUMOCYSTIS JIROVECII, METHOD FOR DETECTING PNEUMOCYSTIS JIROVECII USING SAME AND REAGENT KIT THEREFOR - The invention provides a primer pair for detecting | 2022-09-22 |
20220298587 | NOVEL CONSTRUCT - The invention relates to an NHEJ or MMEJ detection construct, to NHEJ or MMEJ detection assays comprising said construct and to a method of screening for an NHEJ or MMEJ modulator comprising said construct. | 2022-09-22 |
20220298588 | DEVELOPMENT OF A NUCLEIC ACID BASED LATERAL FLOW IMMUNOASSAY FOR BK VIRUS DETECTION FROM ESRD URINES AND CONTAMINATED SEWAGE SAMPLES - A nucleic acid probe set is disclosed. The nucleic acid probe set comprises a detection probe and a capture probe, and the detection probe and the capture probe both include a nucleotide sequence that is extracted from a conserved region of a genome sequence belong to a BK virus. A nucleic acid lateral flow immunoassay for using in detection of BK virus is also disclosed. The nucleic acid lateral flow immunoassay comprises: the forgoing nucleic acid probe set, a test strip, and a streptavidin (SA) solution. Experimental data have proved that, the nucleic acid lateral flow immunoassay can be adopted for conducting a BK virus detection on a sample that is collected from environmental water, sewage water, drinking water, urine, or serum. | 2022-09-22 |
20220298589 | RNA SEPARATION AND RELATED TECHNIQUES FOR DETERMINING VIRUSES SUCH AS CORONAVIRUSES - Certain aspects of the present disclosure generally relate to systems and methods for determining viruses such as coronaviruses. For instance, some aspects are directed to systems and methods for determining viruses using a partitioning system. Within the partitioning system, free RNA or other nucleic acids may preferentially partition into one phase, while intact viruses may be present in the other phase or in both phases. Accordingly, in some cases, free RNA or other nucleic acids may be preferentially removed, e.g., as compared to intact RNA or other nucleic acids present within a virus. In some cases, the phase containing intact viruses can be determined to determine the infectiousness, e.g., of a sample arising from a subject. This may be useful, for example, for distinguishing subjects who are capable of spreading an infection from those who are not infectious. | 2022-09-22 |
20220298590 | PROCESS FOR TANNING LEATHERS, PRE-TANNING PRODUCTS AND USE THEREOF - A process for tanning leathers including a pre-tanning step and a tanning step is provided. In the pre-tanning step leathers are treated with a cationic active agent including a quatemized ester of a fatty acid and triethanolamine, or mixtures thereof and, subsequently, with an anionic active agent including tannins vegetable extracts and a natural or synthetic dispersant selected from the group consisting of lignosulfonates, distillation stillages, sulfonated disulfonic polymers, sulfonated naphthalene polymers or mixtures thereof, to obtain leathers with a shrinkage temperature of at least 65° C. The pre-tanning and tanning steps do not include treatment with aldehydes or aldehyde donor compounds and/or with metal salts. | 2022-09-22 |
20220298591 | TUYERE STOCK, METHOD OF FABRICATING THE SAME, AND AIR BLAST SYSTEM FOR MELTING FURNACE - An air blast system for melting furnaces includes: a supply unit supplying hot air into a melting furnace; a bustle pipe connected to the supply unit; a tuyere stock connecting the bustle pipe to the melting furnace to supply hot air from the bustle pipe to the melting furnace in a distributed manner; and a temperature management module determining whether the tuyere stock is damaged by comparing a temperature of the tuyere stock with a preset reference temperature. | 2022-09-22 |
20220298592 | LOW-STRENGTH STEEL SHEET FOR HOT STAMPING, HOT-STAMPED COMPONENT, AND METHOD FOR MANUFACTURING HOT-STAMPED COMPONENT - A low-strength steel sheet for hot stamping according to an aspect of the present invention satisfies a predetermined chemical composition, the Ac | 2022-09-22 |