37th week of 2012 patent applcation highlights part 44 |
Patent application number | Title | Published |
20120231455 | PEPTIDE NUCLEIC ACID PROBES, KIT AND METHOD FOR DETECTING HELICOBACTER PYLORI AND/OR CLARITHROMYCIN RESISTANCE PROFILE AND APPLICATIONS - Four peptide nucleic acid probes (PNA) are for the detection of | 2012-09-13 |
20120231456 | INSTRUMENTS AND METHODS FOR MIXING THE CONTENTS OF A DETECTION CHAMBER - A receptacle having interconnected chambers arranged to permit multiple process steps to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle. | 2012-09-13 |
20120231457 | COMPOSITIONS AND METHODS FOR ASSESSING A GENETIC RISK OF DEVELOPING LATE-ONSET ALZHEIMER'S DISEASE (LOAD) - The described invention provides compositions and methods for assessing a genetic risk of developing late-onset Alzheimer's disease (LOAD) in a subject by analyzing haplotypes of human Apolipoprotein E (APOE) and Translocase of Outer Mitochondrial Membrane 40 homolog (TOMM40) genes using a PCR- and restriction digest-based approach. | 2012-09-13 |
20120231458 | METHOD OF ACQUIRING STANDARD CURVE IN REAL-TIME PCR - A method of acquiring a standard curve for quantifying polynucleotide is provided. The method includes: (a) performing a real-time polynucleotide chain reaction (PCR) for plural samples having different initial polynucleotide concentrations, the PCR being performed with respect to plural amplification cycle numbers using detectable probes providing a signal according to an amount of polynucleotide; (b) acquiring plural amplification profile curves with respect to signal intensity values provided by the probes according to the amplification cycle numbers; (c) selecting one threshold from among the signal intensity values; (d) calculating amplification cycle numbers corresponding to the selected thresholds from the plural amplification profile curves, and determining the calculated amplification cycle numbers as threshold cycle (Ct) values corresponding to each of the initial polynucleotide concentrations; (e) selecting at least two Ct values among the Ct values determined in (d); and (f) acquiring a standard curve from the selected Ct values. | 2012-09-13 |
20120231459 | CHEMILUMINESCENT PROBES FOR MULTIPLEX MOLECULAR QUANTIFICATION AND USES THEREOF - A novel method is disclosed for simultaneous detection and quantification of two or more nucleic acid targets, without need for amplification. The method depends on spectral-temporal resolution of chemiluminescence emitted from independent hybridization-induced chemiluminescent signal (HICS) probes. The utility of this method has been demonstrated by use of resolvable N-linked acridinium and 2,7-dimethoxyacridinium ester labeled probes in a homogeneous assay for sensitive and simultaneous independent quantification of several bacterial and fungal target sequences. Compositions and kits for practicing the method of the present invention are also disclosed. | 2012-09-13 |
20120231460 | Method and Apparatus for Predicting Pharmacological Efficacy of Human Anti-TNFa Antibody Drug against Rheumatoid Arthritis - A method for predicting pharmacological efficacy of a human anti-TNFα antibody drug against rheumatoid arthritis, the method including: measuring a level of at least one of ADAMTS4 and ADAMTS5 in a sample derived from a subject, and determining whether or not the human anti-TNFα antibody drug is efficacious against rheumatoid arthritis of the subject, based on the level of the at least one of ADAMTS4 and ADAMTS5 serving as an index. | 2012-09-13 |
20120231461 | DETECTION OF NUCLEIC ACIDS - The present invention relates to compositions and methods for the detection and characterization of small nucleic acid molecules (e.g., RNA (e.g., small RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs)) and other short nucleic acid molecules). More particularly, the present invention relates to methods for the detection and quantification of RNA expression. The present invention further provides for the detection of miRNA and siRNA variants. | 2012-09-13 |
20120231462 | ARTIFICIAL BASE PAIR CAPABLE OF FORMING SPECIFIC BASE PAIR - The present invention provides a double-stranded nucleic acid in which at least one nucleic acid strand includes an unnatural base that forms a self-complementary base pair or an unnatural base that forms a base pair with any natural base with substantially the same thermal stability. The present invention also provides a method of hybridizing a first nucleic acid strand with a second nucleic acid strand, wherein the first nucleic acid strand includes an unnatural base that forms a self-complementary base pair or an unnatural base that forms a base pair with any natural base with substantially the same thermal stability, and a method of applying the nucleic acid to SNP detection, a DNA chip, DNA/RNA computing, or an in vitro translation system. The present invention provides a method of introducing an unnatural base into a nucleic acid strand and thereby controlling the thermodynamic stability in hybridization of the nucleic acid strand. | 2012-09-13 |
20120231463 | Primer Set for Amplification of MTHFR Gene, MTHFR Gene Amplification Reagent Containing the Same, and Use of the Same - The present invention provides a primer set for specifically amplifying a target region in a MTHFR gene by a nucleic acid amplification method, a MTHFR gene amplification reagent containing the primer set, and use of the primer set. | 2012-09-13 |
20120231464 | Heatable Droplet Device - A heatable droplet device is used to embody real-time detection by means of the device's temperature control and surface treated and trimmed. A temperature causing internal stability disturbed is immediately detected with a designed sensor while affecting a specific area. | 2012-09-13 |
20120231465 | NUCLEIC ACID QUANTITATION METHOD - The present invention relates to methods of quantifying nucleic acids and in particular to an improved universal method of quantifying nucleic acids for gene expression studies without the need for normalising data to a housekeeping gene or to a synthetic gene of interest. | 2012-09-13 |
20120231466 | METHODS AND COMPOSITIONS FOR ISOLATING NUCLEIC ACID - The present invention relates to compositions and methods for isolating and purifying nucleic acid. In particular, the present invention relates to methods of isolating nucleic acid from cells for use in further analysis. | 2012-09-13 |
20120231467 | Aptamers for C. Difficile Diagnostics - The present disclosure relates generally to the field of nucleic acids and, more particularly, to aptamers capable of binding to toxins produced by | 2012-09-13 |
20120231468 | RNA FROM CYTOLOGY SAMPLES TO DIAGNOSE DISEASE - The invention relates to methods and kits for detecting the likelihood that a subject has cancer, e.g., squamous cell carcinoma, by assaying the expression levels of tumor associated genes. More specifically, the expression levels of nucleic acids or proteins can be assayed in the tumor associated genes, e.g., over-expression of beta-2 microgobulin (B2M), keratin 17 (KRT17), interleukin 8 (IL8), or annexin A2 (ANXA2), and under-expression of cytochrome p450 1B1 (CYP1B1) or laminin gamma-2 (LAMC2) can be indicative of the likelihood a subject has squamous cell carcinoma or a precancerous squamous cell disorder. The expression levels compared to standards can be indicative of the likelihood a subject has squamous cell carcinoma. The expression levels of B2M, CYP1B1, KRT17, IL8, ANXA2, or LAMC2 can also be repeatedly assayed to monitor the progression of a squamous cell neoplasia. | 2012-09-13 |
20120231469 | FORMALIN-FIXED ISOTOPE-LABELED REFERENCE STANDARDS AND METHODS FOR FABRICATION AND USE THEREOF - One or more cells are labeled with minor stable isotopes, characterized, and preserved for subsequent use as a bio-specimen reference standard. The one or more cells are grown in culture media supplied with minor stable isotopes in concentrations substantially different from normally occurring concentrations, thereby supplanting major stable isotopes that would normally be incorporated into the proteins of the cells. The proteins of the cells are thus labeled by the minor stable isotopes and can be used in proteomic characterization of the cells. The cells are preserved by fixation as a reference standard. Cells of the reference standard are mixed with the sample and subject to mass spectrometry evaluation, whereby the labeled proteins of the reference standard can be used in determining the proteome of the sample. | 2012-09-13 |
20120231470 | METHODS AND SYSTEMS ASSOCIATED WITH DETECTION OF FATTY ACID ELONGATION IN A CELL - Methods and systems to identify compounds capable of altering a fatty acid elongation pathway and for identifying conditions under which fatty acids elongation can occur in a cell are described. The methods and systems comprise labeled fatty acid precursors and cells capable of elongating fatty acids. Methods for providing suitable components of an assay for identifying compounds capable of altering a fatty acid elongation pathway are described. | 2012-09-13 |
20120231471 | MARKER ASSOCIATED WITH NON-ALCOHOLIC STEATOHEPATITIS - Disclosed is a novel NASH marker for use in a method for detecting NASH or evaluating the severity of NASH, which utilizes at least one factor selected from the group consisting of an IL-1 receptor antagonist, sCD40, HMGB1, sPLA2 group IIA and an sPLA2 activity as the marker. Also disclosed is a method for detecting NASH or evaluating the severity of NASH in a subject, which utilizes the marker. | 2012-09-13 |
20120231472 | METHODS AND COMPOSITIONS FOR DIAGNOSIS AND PROGNOSIS OF RENAL INJURY AND RENAL FAILURE - The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using a one or more assays configured to detect a kidney injury marker selected from the group consisting of C—C motif chemokine 23, Transmembrane glycoprotein NMB, Brain-derived neurotrophic factor, Cathepsin S, Transforming growth factor beta-2, Urokinase-type plasminogen activator, Angiopoietin-2, Matrilysin, Carcinoembryonic antigen-related cell adhesion molecule 1, Creatine kinase MB, Insulin, Immunoglobulin M, Immunoglobulin E, Macrophage migration inhibitory factor, Galectin-3, Transforming growth factor beta-3, Heparan sulfate, soluble Cadherin-3, Complement C5, Platelet factor 4, Platelet basic protein, and Stromelysin-2 as diagnostic and prognostic biomarkers in renal injuries. | 2012-09-13 |
20120231473 | FLOW CYTOMETRY METHOD THROUGH THE CONTROL OF FLUORESCENCE INTENSITIES - Provided is a flow cytometry method including adjusting cell populations targeted by antibodies conjugated with a same-color fluorochrome to show different fluorescence intensities according to types of the antibodies. Unlike a conventional flow cytometry method capable of classifying a positive and negative of one target using one antibody per color, the flow cytometry method adjusts several types of antibodies conjugated with a single-color fluorochrome to respectively show different fluorescence intensities, or adjusts the amounts of antibodies conjugated with a fluorochrome differently according to types of the antibodies, thereby classifying a positive and negative of multiple targets using one color. Accordingly, even when a current flow cytometer capable of classifying a limited number of colors is used, it is possible to classify a variety of cell populations to be clinically examined. | 2012-09-13 |
20120231474 | CONSTRUCTS THAT ALLOW FOR DETECTION AND QUANTITATION OF MEMBRANE PROTEINS - A construct is described that allows for detection and quantitation of membrane-bound polypeptides. | 2012-09-13 |
20120231475 | IMMUNOASSAY FOR DETERMINING THE RELEASE OF NEUROTENSIN INTO THE CIRCULATION - The invention relates to an immunodiagnostic determination method for determining the release of neurotensin into the circulation of mammals during which an immunoreactivity of the N-terminal portion of a mammal proneurotensin (PNT immunoreactivity) is selectively determined in a serum sample or plasma sample of a test mammal, this immunoreactivity not being a neurotensin or neuromedin immunoreactivity. | 2012-09-13 |
20120231476 | METHODS AND COMPOSITIONS FOR DIAGNOSIS AND PROGNOSIS OF RENAL INJURY AND RENAL FAILURE - The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using a one or more assays configured to detect a kidney injury marker selected from the group consisting of Cathepsin B, Renin, Dipeptidyl Peptidase IV, Neprilysin, Beta-2-microglobulin, Carbonic anhydrase IX, and C-X-C motif chemokine 2 as diagnostic and prognostic biomarkers in renal injuries. | 2012-09-13 |
20120231477 | Blood Biomarkers for Bone Fracture and Cartilage Injury - Blood biomarkers are described for use in methods and compositions to determine whether an individual has sustained a bone fracture or a cartilage injury. | 2012-09-13 |
20120231478 | TIVOZANIB RESPONSE PREDICTION - A diagnostic method for predicting whether a human tumor will be sensitive or resistant to treatment with tivozanib (AV-951) is disclosed. The method is based on measurement of macrophage content in a tissue sample from a tumor. Measurement of macrophage content can be based on analysis of macrophage marker gene expression, e.g., by RNA analysis or immunohistochemistry. | 2012-09-13 |
20120231479 | COMBINATION METHODS OF DIAGNOSING CANCER IN A PATIENT - The present disclosure relates to methods for determining the presence, activity, and/or concentrations of certain cancer biomarkers and their use in determining the presence of cancer. | 2012-09-13 |
20120231480 | MATERIALS AND METHODS FOR THE DIFFERENTIAL DIAGNOSIS OF PACREATIC LESIONS - Levels of VEGF-A, VEGF-C and PGE | 2012-09-13 |
20120231481 | METHODS OF EVALUATING BAFF - The present disclosure provides compositions and methods relating to the evaluation of BAFF in a biological sample from a subject. | 2012-09-13 |
20120231482 | METHOD, KIT OR DIAGNOSTIC FOR THE DETECTION OF REAGENTS WHICH INDUCE ALTERED CONTRACTILITY - A method of screening for compounds that enhance or depress contractile function, based on measuring the formation of heterodimers of contractile fibers (e.g. Tm and actin, myosin heavy and myosin light chains), for example through disulfide bond formation. Diagnostic and prognostic methods and kits are also provided. | 2012-09-13 |
20120231483 | METHOD AND APPARATUS FOR REDUCING LUMINESCENT TEST RESULT INTEREFERENCES (CONTINUATION) - This application involves detecting luminescence. Various methods and devices are described that reduce interference of ambient light, including UV radiation, on test results. Such methods and devices include using UV blocking material and covering test components prior to use. | 2012-09-13 |
20120231484 | METHOD AND MARKER FOR DETERMINATION DEGREE OF RISK OF ONSET OF HIGH-FUNCTIONING AUTISM - The present invention relates to a method for determining the degree of risk of onset of autism, comprising the step of measuring the triglyceride concentration or the cholesterol concentration in a very low-density lipoprotein fraction of plasma or serum isolated from a subject, or the triglyceride concentration or the cholesterol concentration of plasma or serum. In addition, the present invention provides a kit for determining the degree of risk of onset of autism and a method for screening for a candidate substance for agents for treating autism using a non-human mammal, in which the above described method is utilized. | 2012-09-13 |
20120231485 | METHOD FOR MONITORING ANTICOAGULANT THERAPY - A method of measuring the combined activity of both and only coagulation factors II and X for the purpose of monitoring anticoagulant therapy, and kits for using the method. The method involves mixing of test plasma from a human to be tested with specially prepared plasma deficient in both and only coagulation factors II and X but with normal levels of other factors (referred to herein as Fiix-deficient plasma or Fiix plasma), in order to correct for any possible deficiency in other coagulation factors than FII and FX in the test sample. By adding a coagulation reagent and calcium, the generation of thrombin or fibrin can be measured. Kits of the invention comprise a coagulation reagent, calcium and specially made plasma that is deficient in both and only factor II and factor FX. | 2012-09-13 |
20120231486 | METHOD AND APPARATUS TO PERFORM HYDROGEN-DEUTERIUM EXCHANGE - Apparatus and methods to perform hydrogen/deuterium exchange using semipermeable membranes are described. The system has two channels separated by a semipermeable membrane. One channel comprises a flow carrying the analyte of interest, and the second channel comprises a solution comprising a deuterated solvent (e.g. deuterium oxide). The system does not require an external electric field gradient across the membrane to perform the hydrogen-deuterium exchange procedure. The present invention facilitates sample and reagent handling as well as simplifies manufacture of devices and/or instrumentation related to deuterium exchange. | 2012-09-13 |
20120231487 | Method for preparing an invasive test of an egg and for determining a gender of an embryo in an egg - The present invention relates to a method for preparing an invasive test on an egg, such as determining the gender of an embryo in an egg, comprising the step of: providing a passage to the interior of an egg. The present invention also relates to a method for determining the gender of an embryo in an egg. | 2012-09-13 |
20120231488 | FLUID SAMPLE COLLECTION DEVICE - Disclosed herein is a sample collection device for an aqueous fluid, such as whole blood, serum, plasma and urine. The device comprises a cartridge body defining an elongate sample collection passage that has open ends. The passage is arranged to draw the fluid into the passage by capillary action. The passage is provided along a portion of its length with a sample metering stop in the form of a hydrophobic coating arranged to prevent flow of the fluid by capillary action thereacross. A sample receiving portion of the passage extending between a collection end and the metering stop is non-linear, and preferably defines a pair of straight limbs connected by a bend. By providing a non-linear passage in this way, the maximum gravitational force which can act on the collected sample is reduced as compared to a conventional linear passage, thereby reducing the tendency of the sample to leak from the device and potentially avoiding the need for one or both ends of the passage to be sealed. The sample receiving portion of the passage may be provided with a hydrophilic coating to enhance the capillary action. A particularly suitable hydrophilic coating for a whole blood sample collection device is heparin, which may also serve as an anticoagulant for the blood. | 2012-09-13 |
20120231489 | IRIDESCENT SURFACES AND APPARATUS FOR REAL TIME MEASUREMENT OF LIQUID AND CELLULAR ADHESION - Described is a method and apparatus for determining the adhesion of an object to an iridescent surface based on the detected scattered light scattered by the interface region for the iridescent surface and the object. | 2012-09-13 |
20120231490 | Method Of Differentiation From Stem Cells To Hepatocytes - Disclosed are: a gene transduction method for use in the induction of the differentiation of stem cells such as ES cells or iPS cells into hepatocytes effectively; stem cells into each of which a gene useful for the induction of the differentiation into hepatocytes is introduced; and hepatocytes produced from stem cells each having the gene introduced therein. A specific gene can be introduced into stem cells such as ES cells or iPS cells using an adenovirus vector. The effective induction of the differentiation into hepatocytes can be achieved by introducing the gene. Specifically, the effective induction of the differentiation of stem cells such as ES cells or iPS cells into hepatocytes can be achieved by introducing at least one gene selected from HEX gene, HNF4A gene, HNF6 gene and SOX17 gene into the stem cells. | 2012-09-13 |
20120231491 | METHOD FOR EVALUATING TOXICITY OF CHEMICAL USING ALGA - The present invention provides a method for evaluating the toxicity of a chemical by using an alga, comprising: (a) a thawing step of thawing frozen algal cells by heating, and diluting the obtained suspension of the cells by adding a culture medium thereto; (b) a recovery culture step of culturing the algal cells obtained in the thawing step (a) to allow the algal cells to recover from the effects of freezing and thawing; (c) a confirmation step of collecting a part of the algal cells after the recovery culture step (b), diluting the part of the algal cells by adding a culture medium thereto, and measuring the amount of the luminescence of the delayed luminescence of the algal cells as initial value data; (d) an exposure step of mixing the algal cells after the confirmation step (c) with a solution containing a test substance to prepare an exposure sample, and culturing the exposure sample; (e) a measurement step of measuring the amount of the luminescence of the delayed luminescence of the exposure sample after the exposure step (d) as exposure data; and (f) an evaluation step of calculating an evaluation value based on the initial value data and the exposure data, and evaluating the toxicity of the test substance based on the evaluation value. | 2012-09-13 |
20120231492 | SENSOR FOR DETECTING MICROORGANISMS AND CORRESPONDING PROCESS - Microbial fuel cells generate an electrical signal when microbes enter the cells through a semipermeable membrane. By reading and analyzing the signal from one or more such fuel cells can indicate infection in people or animals, indicate pathogens growing in food or show mold growth. Insofar as different microbes have specific metabolisms, the signal may be used to determine which microbe is present. | 2012-09-13 |
20120231493 | APPARATUS FOR CHEMILUMINESCENT ASSAY AND DETECTION - An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member, and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit, and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less. | 2012-09-13 |
20120231494 | COMBINED LIQUID TO SOLID-PHASE ANAEROBIC DIGESTION FOR BIOGAS PRODUCTION FROM MUNICIPAL AND AGRICULTURAL WASTES - Biogas is obtained in a solid-state anaerobic digester from a solid organic biomass and a digestion effluent produced in an associated liquid anaerobic digester. The solid organic biomass and digestion effluent are mixed to produce an effluent-biomass mixture. The effluent-biomass mixture is then incubated in the solid state anaerobic digester, producing a biogas and a digestate. The incubation is controlled by adjusting the composition and properties of the effluent-biomass mixture. The solid organic biomass may comprise lignocellulosic biomass, food waste, agricultural waste and the like. | 2012-09-13 |
20120231495 | PRODUCTION OF NON-YEAST STEROLS BY YEAST - This invention relates to the production of 7-dehydrocholesterol, 25-hydroxy-7-dehydrocholesterol, and 25-hydroxy ergosterol in yeast such as | 2012-09-13 |
20120231496 | COMPOSITION FOR SYNTHESIZING PROTEIN WITH REDUCED LIPOPOLYSACCHARIDE CONTAMINATION, METHOD FOR PRODUCING PROTEIN USING SAID COMPOSITION - According to the present invention, a composition possessing cell-free protein synthesis activity with reduced contaminating lipopolysaccharide, and a method for producing a protein using the same are provided. When ribosome display is performed using the composition and method for protein production of the present invention, the background that is caused by non-specific binding is reduced, so that a nucleic acid that encodes the desired polypeptide can be selected with high accuracy and high efficiency. | 2012-09-13 |
20120231497 | SELECTIVE ENZYMATIC HYDROLYSIS OF C-TERMINAL TERT-BUTYL ESTERS OF PEPTIDES - The present invention relates to a process for the selective enzymatic hydrolysis of C-terminal esters of peptide substrates in the synthesis of peptides, comprising hydrolysing C-terminal tert-butyl esters using the protease subtilisin. This process is useful in the production of protected or unprotected peptides. | 2012-09-13 |
20120231498 | PROCESSING ENZYMES FUSED TO BASIC PROTEIN TAGS - The invention is related to processing enzyme comprising an N-terminally attached tag derived from highly basic proteins from thermophilic bacteria. The processing enzymes are useful for modifying proteins. They can be produced in high yields and can be effectively separated from the modified protein after use. | 2012-09-13 |
20120231499 | HIGH-MOLECULAR-WEIGHT RECOMBINANT SILK OR SILK-LIKE PROTEIN AND MICRO- OR NANO-SIZED SPIDER SILK OR SILK-LIKE FIBER PRODUCED THEREFROM - A high-molecular-weight recombinant silk or silk-like protein having a molecular weight which is substantially similar to that of native silk protein, and a micro- or nano-sized spider silk or silk-like fiber having improved physical properties, produced therefrom. The recombinant silk or silk-like protein according to the invention has high molecular weight, like dragline silk proteins from spiders, while a fiber produced therefrom has excellent physical properties compared to a fiber produced from native silk protein. Thus, the recombinant silk or silk-like protein and the spider silk or silk-like fiber produced therefrom will be highly useful in various industrial applications, including bioengineering applications and medical applications. | 2012-09-13 |
20120231500 | Cells For Transient Expression And Uses Thereof - This invention relates to the transient expression of heterologous polypeptides in mammalian cell lines. Specifically it relates to an expression-enhanced cell line derived from a parent cell line, the expression-enhanced cell line comprising nucleic acid encoding Epstein-Barr Virus Nuclear Antigen 1 or a functional derivative, analogue, or variant thereof; and further comprising:
| 2012-09-13 |
20120231501 | Methods For Increasing Homologous Recombination Of A Nucleic Acid Sequence - The present invention relates to methods for increasing homologous recombination of a nucleic acid sequence introduced into a host cell, comprising: (a) introducing into a population of filamentous fungal host cells a first nucleic acid sequence encoding a recombination protein and a second nucleic acid sequence comprising one or more regions which are homologous with the genome of the filamentous fungal host cell, wherein (i) the recombination protein promotes the recombination of the one or more regions with the corresponding homologous region in the host's genome to incorporate the second nucleic acid sequence by homologous recombination, and (ii) the number of host cells comprising the incorporated second nucleic acid sequence in the population is increased at least 20% compared to the same population without the first nucleic acid sequence; (b) and isolating from the population a filamentous fungal cell comprising the incorporated second nucleic acid sequence. | 2012-09-13 |
20120231502 | METHOD FOR PRODUCING THERAPEUTIC PROTEINS IN PICHIA PASTORIS LACKING DIPEPTIDYL AMINOPEPTIDASE ACTIVITY - The present invention related to methods and compositions for producing therapeutic proteins in yeast cell lines, and in particular | 2012-09-13 |
20120231503 | Gene Expression Technique - The present disclosure relates to a method for producing heterologous protein including:
| 2012-09-13 |
20120231504 | MULTIUSE REACTORS AND RELATED METHODS - A septum is positioned within a disposable vessel and defines a lower chamber and an upper chamber. The septum includes a plurality of apertures that provide fluid communication between the upper chamber and lower chamber. Compressed gas is introduced in the lower chamber to produce fine bubbles rising up throughout the vessel to produce a mixing and gasification needed for the growth of a biological culture and manufacture of a biological product in a nutrient medium. Adding a binding resin to the upper chamber allows harvesting, separation and purification of biological products in the reactor as a single unit operation. | 2012-09-13 |
20120231505 | Gene Expression Technique - The present invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising:
| 2012-09-13 |
20120231506 | MULTISTEP FINAL FILTRATION - Herein is reported a method for the final filtration of concentrated polypeptide solutions comprising the combination of two immediately consecutive filtration steps with a first filter of 3.0 μm and 0.8 μm pore size and a second filter of 0.45 μm and 0.22 μm pore size. | 2012-09-13 |
20120231507 | NOVEL CELLULASES WITH HIGH ACTIVITIES - Two novel cellulases and nucleotide sequences encoding the same are disclosed. Also disclosed are compositions and methods for using the same for hydrolyzing cellulosic waste materials. | 2012-09-13 |
20120231508 | NOVEL MULTIPLEX BARCODED PAIRED-END DITAG (mbPED) SEQUENCING APPROACH AND ITS APPLICATION IN FUSION GENE IDENTIFICATION - A method of generating a barcoded Paired-End Ditag (bPED) nucleic acid fragment is disclosed. The method comprises: a) performing a first ligation by ligating a half-adaptor with one or two 3′-overhanging ends to a target nucleic acid to obtain a nucleic acid fragment with two ends each attached to one of the half-adaptor, the half adaptor comprising a half-barcode and a restriction enzyme (RE) recognition site; b) performing a second ligation by ligating two of the half-adaptor at the two ends of the nucleic acid fragment to form a circularized nucleic acid construct, wherein the circularized nucleic acid construct comprises a full-size barcoded adaptor; and c) digesting the circularized nucleic acid construct with a RE that cleaves at a defined distance from the RE recognition site, and thereby generating the bPED nucleic acid fragment. | 2012-09-13 |
20120231509 | Primers and Probes for the Amplification and Detection of HIV GAG, REV and NEF Polynucleotides - The invention relates to improved methods and compositions for the nucleic acid amplification of one or multiple variants (strains) of Human Immunodeficiency Virus (HIV) present in a sample, and preferably in a sample from a pathogen infected individual. In particular, novel primers, methods and kits for the amplification of one or more species of HIV Rev, Gag and Nef nucleic acids are provided. The amplified HIV nucleic acid can be used to identify and/or quantitate HIV variants present in a sample. Nucleic acids produced by the methods of the invention or the proteins encoded thereby can also be used directly as vaccines or to transfect/load antigen presenting cells. The loaded antigen presenting cells can be used as a vaccine for the treatment or prevention of HIV infection. | 2012-09-13 |
20120231510 | MULTI-CELLULASE ENZYME COMPOSITIONS FOR HYDROLYSIS OF CELLULOSIC BIOMASS - The invention relates to a multi-cellulase enzyme composition for the enzymatic hydrolysis of cellulosic biomass said composition comprising a cellobiohydrolase (CBH) enzyme, an endoglucanase (EG) enzyme and a β-glucosidase (BG) enzyme. | 2012-09-13 |
20120231511 | PREPARATION METHOD FOR BIO-FUEL MATERIALS AND BIO-CHEMICALS - Disclosed is a preparation method for bio-fuel materials and bio-chemicals comprising the following steps: preparing a medium comprising fermentation waste generated in an alcohol production process; inoculating a first microorganism into the medium; and culturing the medium wherein the first microorganism was inoculated. More specifically, the preparation method for bio-fuel materials and bio-chemicals comprises the following steps: fermenting hexoses from a mixture of pentoses and hexoses to produce an ethanol fermentation broth; separating and purifying the ethanol fermentation broth; preparing a medium comprising the fermentation waste produced in the separation and purification step; inoculating a first microorganism into the medium; and culturing the medium wherein the first microorganism was inoculated. | 2012-09-13 |
20120231512 | PREPARATION OF ALPHA-KETOPIMELIC ACID - The present invention relates to a method for preparing alpha-ketopimelic acid, comprising converting 2-hydroxyheptanedioic acid into alpha-ketopimelic acid, which conversion is catalysed using a biocatalyst. Further, the invention relates to a heterologous cell, comprising a nucleic acid sequence encoding an enzyme having catalytic activity in the conversion of 2-hydroxyheptanedioic acid into alpha-ketopimelic acid. Further, the invention relates to the use of a heterologous cell according to the invention in the preparation of caprolactam, diaminohexane or adipic acid. | 2012-09-13 |
20120231513 | SYSTEMS AND METHODS FOR CULTURING ALGAE WITH BIVALVES - Provided herein are systems and methods for extracting lipids and/or producing biofuel from algae in marine and freshwater environments, wherein algae and bivalves are co-cultured in a system of enclosures comprising water that comprises recycled nutrients that are essential for algal growth. The system also include enclosures for culturing fishes which are used to harvest the algae. | 2012-09-13 |
20120231514 | FERMENTATION METHOD TO PRODUCE A LIGNOCELLULOSE-BASED SUGAR STREAM WITH ENRICHED PENTOSE CONTENT - A method for producing a fermented solution enriched in xylose is provided. The sugar stream that is fermented results from the hydrolysis of a lignocellulosic feedstock and comprises at least a hexose and a pentose. The method comprises fermenting the sugar stream resulting from the hydrolysis of the lignocellulosic feedstock with a microorganism that preferentially ferments the hexose over the pentose. The fermenting is conducted under aerobic conditions and comprises continuously feeding the sugar stream to a fermentation reactor at a dilution rate that converts the hexose to cell mass preferentially over the pentose, thereby reducing the concentration of the glucose in the sugar stream so that the fermented solution thus produced is enriched in the pentose relative to said sugar stream. | 2012-09-13 |
20120231515 | BUTANOL DEHYDROGENASE ENZYME FROM THE BACTERIUM ACHROMOBACTER XYLOSOXIDANS - From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of | 2012-09-13 |
20120231516 | ADAPTED CULTURE FOR CELLULOSIC FERMENTATION - A method for producing ethanol by fermentation includes the preparation of a starter culture, inoculation of a mash with the starter culture, fermentation of mash, and recovery of ethanol from the mash. The starter culture includes a tallow base with Chinese tallow tree parts and water which are inoculated with micro-organisms, where the micro-organisms include yeast. The micro-organisms are grown in the tallow base, and used to inoculate the mash. The mash is then fermented, and ethanol is recovered from the mash. | 2012-09-13 |
20120231517 | MINIATURIZED ELECTROPORATION-READY MICROWELL ARAY FOR HIGH-THROUGHPUT GENOMIC SCREENING - Methods of introducing exogenous molecules into cells including cell lines and primary cells are provided. Additionally, miniaturized electroporation-ready microwell arrays are provided. These tools provide a miniaturized high-throughput functional genomics screening platform to carry out genome-size screens in a variety of cell types. | 2012-09-13 |
20120231518 | COMPOSITIONS AND METHODS FOR MODIFYING A SILICONE SURFACE FOR PROLONGED INTERFERENCE AGAINST PATHOGEN COLONIZATION - According to some embodiments, the present invention provides a modified silicone surface for interference to pathogen colonization comprising: an activated silicone layer; a plurality of cross-linking dendrimers adsorbed onto to the activated silicone layer; a plurality of ligand derivatives, each bound to at least one of the plurality of cross-linking dendrimers; and a benign biofilm adhered to the plurality of ligand derivatives. According to some embodiments, the present invention provides a method for making a modified silicone surface for interference to pathogen colonization comprising activating a silicone surface; adsorbing a plurality of cross-linking dendrimers to the silicone surface; binding a plurality of ligand derivatives to the plurality of cross-linking dendrimers; and adhering a benign biofilm to the plurality of ligand derivatives. | 2012-09-13 |
20120231519 | Molecular Surface Design of Tyrosine-Derived Polycarbonates for Attachment of Biomolecules - Methods for constructing tyrosine-derived biotinylated polymers. Biotinylated polymers and polymer scaffolds constructed with the biotinylated polymers are also disclosed. | 2012-09-13 |
20120231520 | NOVEL FUSION PROTEINS AND METHOD OF EXPRESSION THEREOF - The present invention relates to novel Prolipase-Bovine trypsinogen (PLBTR) fusion proteins, the genes encoding them, and the production and uses thereof. More specifically, the present invention relates to methods of producing in optimal quantities PLBTR fusion proteins which comprise a heterologous polypeptide which is normally susceptible to autocatalytic activity. More particularly, the present invention relates to fusion proteins which comprise an heterologous polypeptide, such as a serine protease, fused to a lipase signal sequence, which can be expressed by recombinant host cells in desired amounts. The present invention further relates to polynucleotides encoding such fusion proteins, to expression vectors for expression of such fusion proteins, to host cells transformed with such polynucleotides/vectors, and to methods of generating such fusion proteins. | 2012-09-13 |
20120231521 | Transducible Polypeptides For Modifying Metabolism - Methods and compositions for modifying the metabolism of a subject are provided. One embodiment provides a recombinant polypeptide having a polynucleotide-binding domain, a protein transduction domain, and a targeting domain. In a preferred embodiment, the polynucleotide-binding domain includes one or more HMG box domains. | 2012-09-13 |
20120231522 | Amplicon Melting Analysis With Saturation Dyes - Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided. | 2012-09-13 |
20120231523 | RECOMBINANT FACTOR X WITH NO GLYCOSYLATION AND METHOD FOR PREPARING THE SAME - A Factor X (hereinafter referred to as “FX”) with a high activity is provided. The present invention relates to a method for efficiently preparing a recombinant, two-chain FX which comprises intervening glycosylation at such an amino acid sequence that is essential for glycosylation in FX to thereby allow for expression of a recombinant FX with no glycosylation, and the recombinant FX with no glycosylation obtained by said method. | 2012-09-13 |
20120231524 | CHIMERIC ADENOVIRUSES FOR USE IN CANCER TREATMENT - The present invention relates to oncolytic adenoviruses having therapeutic applications. Recombinant chimeric adenoviruses, and methods to produce them are provided. The chimeric adenoviruses of the invention comprise nucleic acid sequences derived from adenoviral serotypes classified within the subgroups B through F and demonstrate an enhanced therapeutic index. | 2012-09-13 |
20120231525 | MULTIVALENT PHAGE DISPLAY SYSTEMS AND METHODS - The present invention relates to vectors, methods and systems for polypeptide display and selection. Specifically, the present invention relates to vectors, methods, and systems for multivalent phage display using pIX protein of filamentous phage and helper phage. | 2012-09-13 |
20120231526 | Method for the Purification of Alphavirus Replicon Particles - Methods of production and purification for viruses and virus-derived vectors, including those related to alphaviruses, are disclosed. In one aspect, methods of purification that subject alphavirus replicon particle preparations to one or more steps of chromatographic purification, such as using an ion exchange resin, are provided. Also disclosed are methods of characterizing alphavirus replicon particles and utilizing these materials for vaccines and gene-based therapeutics | 2012-09-13 |
20120231527 | SYSTEM AND PROCESS FOR THE TREATMENT OF GAS EMISSIONS AND EFFLUENTS, AND PRODUCTION OF ALGAL BIOMASS - The present application generally relates to a process for the reduction of gas emissions, treatment of effluents and production of algal biomass, and to a system for the reduction of gas emissions, treatment of effluents and production of algal biomass. | 2012-09-13 |
20120231528 | REACTION CASING FOR A PHOTOSYNTHETIC REACTOR AND ASSOCIATED PHOTOSYNTHETIC REACTOR - “A reaction casing for a photosynthetic reactor designed firstly to float on a body of water and secondly to delimit a biphasic flow pathway for gas/liquid culture medium between a first and a second opening of the casing, where the casing includes two claddings, respectively outer and inner, made at least in part of a material transparent to light rays, the inner cladding extending inside the outer cladding so that the claddings delimit between them an inter-cladding space in fluid connection with the first opening of the casing, the outer cladding has an open proximal end and a closed distal end and in the inner cladding has an open proximal end in fluid connection with the second opening of the casing and a distal end provided with at least one communication orifice between the inside of the inner cladding and the inter-cladding space.” | 2012-09-13 |
20120231529 | FLUID CONCENTRATOR WITH REMOVABLE CARTRIDGE - A fluid concentrator includes a main housing and a cartridge removably engaged to the main housing. The main housing includes a first end portion having a first port and a second port, an oppositely disposed second end portion and a separation chamber that extends between the first and second end portions. The second end portion has an inlet port and defines a cartridge passage that extends through the second end portion. The separation chamber is in fluid communication with the inlet port. The cartridge includes a first axial end portion that is engaged with the first end portion and an oppositely disposed second axial end portion, a portion of the second axial end portion is disposed in the cartridge passage of the second end portion of the main housing. The cartridge is adapted to filter fluid from the separation chamber. | 2012-09-13 |
20120231530 | METHODS OF CHROMOSOME DRYING AND SPREADING - The present invention provides for a method of drying and spreading chromosomes from various biological samples to yield optimal chromosomal spreading. The method requires preparing a biological sample for treatment, providing a cytogenetic chamber capable of setting predetermined conditions, pre-testing a portion of the biological sample in the cytogenetic chamber, and finally treating the remaining biological sample. The method is useful to yield metaphase chromosomes that are small and rounded, with very few overlapping or scattered chromosomes. Furthermore, the method is uses restricted ranges of temperature and relative humidity to achieve consistent chromosomal spreading. The morphologies of the chromosomes are preserved in order to execute banding techniques at 550 bands and chromosomal analysis on high-resolution chromosomes. | 2012-09-13 |
20120231531 | BIOCHEMICAL DETECTION UNIT AND BIOCHEMICAL DEVICE HAVING THE SAME - A biochemical detection unit for detecting a sample and a biochemical device having the biochemical detection unit and a releasing unit are provided. The biochemical detection unit includes a photoconductor plate, a receptor, and a resistance sensing component. The receptor specifically binds to the sample so that the illumination projected on the photoconductor plate will change to vary the resistance value of the photoconductor of the photoconductor plate. | 2012-09-13 |
20120231532 | OPTICAL SCANNING SYSTEM - An optical scanning system including a switchable light source, a detector, a substrate and a plurality of optical sensing sites, as well as methods and kits for use thereof are provided. The substrate is coupled to and in optical communication with the switchable light source and the detector. Additionally, the substrate includes a plurality of substantially parallel excitation waveguides, and a plurality of substantially parallel collection waveguides, the excitation waveguides and collection waveguides crossing to form a two-dimensional array of intersection regions where an excitation waveguide and a collection waveguide cross and provide optical communication with the intersection region at each crossing. The plurality of optical sensing sites are each in optical communication with an intersection region. | 2012-09-13 |
20120231533 | DEVICE AND METHOD FOR THE STUDY OF CELL AND TISSUE FUNCTION - A chamber device for analyzing living cell(s). The chamber device includes a base and a lid that when reversibly pressed closed create a chamber. The base is configured with an optically transparent well to contain at least one cell. The lid has a breadth greater than the base and is configured to contain at least one sensor. The lid is further configured with a lip that when pressed between the lid and the base creates an impermeable seal. The base and the lid are configured so that, when closed and in use, the sensor remains spatially apart from the at least one cell. | 2012-09-13 |
20120231534 | Spectrometric device - The present invention provides improved spectrometric devices useful for measuring optical quantities of a component, including a solid state LED emitter, having at least one wavelength that is matched to the wavelength that is useful for the spectral analysis of the component of interest, a photodetection cell and an optimizing configuration and permits control of the LED emitter and the detector sensitivity to provide a range of detection for the signals corresponding to the optical density of a sample being analyzed and that affords sensitivity for a desired component of a sample by minimizing the error associated with electronic components and signals. Preferred embodiments also include configurations for determining an anticoagulant therapy value that may be used to determine treatment for a patient. | 2012-09-13 |
20120231535 | Organic Forward Osmosis System - An organic forward osmosis system includes an acid treatment stage comprising an acid treatment operation configured to produce an acid treated, digester centrate stream in a forward osmosis reject loop and a fertilizer stream. A forward osmosis stage is coupled to the acid treatment stage and includes a forward osmosis operation configured to remove water from the acid treated, digester centrate stream by: diverting the acid treated, digester centrate stream to one side of at least one forward osmosis membrane; and contacting an opposite side of the at least one forward osmosis membrane with a salt brine stream in a forward osmosis draw loop and osmotically pulling water across the at least one forward osmosis membrane from the acid treated, digester centrate stream to the salt brine stream using only a concentration gradient; thereby producing a concentrated, acid treated, digester centrate stream and a diluted salt brine stream. | 2012-09-13 |
20120231536 | CHIMERAL INTERNAL RIBOSOMAL ENTRY SITE SEQUENCE AND USES THEREOF - An improved baculovirus vector capable of expressing genes in mammalian or insect host cells, and the uses thereof are disclosed. The improved baculovirus vector includes in sequence: a promoter; a first nucleic acid operably linked to the promoter for expressing a first protein in the mammalian or insect host cells; a chimera internal ribosomal entry site (IRES) comprising a portion of an enterovirus (EV) IRES sequence at least 90% identical to SEQ ID NO: 1 and a portion of a | 2012-09-13 |
20120231537 | Highly Pure Plasmid DNA Preparations - The present disclosure generally relates to highly pure plasmid compositions having low, or undetectable, levels of colanic acid and other contaminants made by a process that comprises purifying plasmid DNA by chromatography, treating the purified plasmid DNA with a polypeptide that digests colanic acid under conditions that digest the colonic acid, and separating the plasmid DNA from the digested colonic acid. | 2012-09-13 |
20120231538 | LIPOPHILIC DYE-BASED FRET ASSAYS FOR CLOSTRIDIAL TOXIN ACTIVITY - Compositions useful for detecting Clostridial toxin activity comprising a cell that comprises a membrane-associated Clostridial toxin substrate comprising a first member of a fluorescence resonance energy transfer pair; and a Clostridial toxin recognition sequence including a cleavage site; and a membrane-associated second member of the FRET pair and methods useful for determining Clostridial toxin activity using such Clostridial toxin substrates. | 2012-09-13 |
20120231539 | Replication Stable and RNase Resistant Chimeras of Pestivirus with Insertion in 3' Nontranslated Region (3'NTR) - The invention relates to the field of nucleic acid amplification, particularly to quality control materials for use in viral RNA assays. It specifically relates to the construction of a recombinant Pestivirus by the identification of a region in the 3′NTR of the viral RNA genome where additional sequence elements can be stably inserted. Chimeric Pestivirus with sequence insertions in the 3′ nontranslated region (3′NTR) of the viral RNA genome were stable in replication and capable of forming infectious, RNase resistant virus particles. This chimeric Pestivirus with a 3′NTR insertion can be utilized as a quality control material in analytical assays for RNA targets, including external, internal controls, quantitative standards in PCR and NAT nucleic acid assays. | 2012-09-13 |
20120231540 | Non-Simian Cells for Growth of Porcine Reproductive and Respiratory Syndrome (PRRS) Virus - Disclosed are compositions and methods relating to growth of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) using non-simian cells. In a particular example, porcine alveolar macrophage cells are described as having a capability of supporting infectivity and reproduction by PRRSV. Cells and cell lines of the invention are disclosed in connection with applications relating to PRRS disease, including vaccine technologies. | 2012-09-13 |
20120231541 | FLUORESCENCE POLARIZATION hERG ASSAY - Disclosed are assays, methods, and kits for the screening of test compounds for their capability to induce cardiotoxicity in a subject. In particular, whether a test compound has the effect to prolong the Q-T interval as measured by an electrocardiogram in a human. The assays, methods, and kits disclosed herein make use of the binding interaction between novel fluorescent tracers and the hERG K | 2012-09-13 |
20120231542 | Biologically Active Human Umbilical Cord Blood Cell Extract Compounds and Methods - Compositions and methods for culturing therapeutic cells are provided herein. According to at least one embodiment, compositions comprising cord blood plasma and lysed platelets and methods for making and using same are provided herein. | 2012-09-13 |
20120231544 | CELL GROWTH APPARATUS AND USE OF AEROGELS FOR DIRECTED CELL GROWTH - This invention describes a cell growth apparatus, particularly neuronal printed circuit board apparatus comprising an aerogel base and a pre-printed cellular growth pattern. The cellular growth pattern is comprised of combinations of layers of cellular adhesion promoting materials, cellular adhesion inhibiting materials, and/or cellular signal promoting materials. The invention further describes methods of promoting cell growth using the neuronal printed circuit board apparatus of the invention. The invention is useful for regeneration and precise guidance of cells, particularly nerve cells, when used as an implant. | 2012-09-13 |
20120231545 | METHOD FOR MODIFYING BOVINE EMBRYO STEM CELLS AND METHOD FOR PURIFYING PROTEINS PRODUCED BY MODIFIED BOVINE EMBRYO STEM CELLS - The present invention relates to the modification process of bovine embryonic stem cells and purification process of proteins generated by modified stem cells. In particular, the present invention lies in the field of medicine and veterinary. | 2012-09-13 |
20120231546 | NOVEL ACETYL CoA CARBOXYLASES - Provided herein are novel ACCases and nucleotides encoding the same, that when introduced into a cell or organism results in an increase and/or accumulation of fatty acids, glycerol lipids, and/or oils in the cell or organism, and/or a change in the types of fatty acids, glycerol lipids, and/or oils that are normally present in the cell or organism. Also provided herein are organisms transformed with the novel ACCases. | 2012-09-13 |
20120231547 | Reusable End-Capped Bipyridine Compound Useful For Selective Detection Of Cyanide Anions And Process For Preparation Thereof - The present invention describes the development of a end-capped bipyridine compound having formula A and the zinc complex having formula B. The assay having formula 1 can be used to estimate and quantify the amount of zinc ions by monitoring the fluorescence changes. The assay with formula 1 can be use to image and detect Zn | 2012-09-13 |
20120231548 | BREATH KETONE DETECTOR - Ketoacidosis is an extreme and uncontrolled form of ketosis, which is a normal response to prolonged fasting. Embodiments of this invention test the ketone level of a patient by measuring the ketone bodies in breath condensation. Some embodiments include a device for medical testing comprising a hollow container, comprising powder mixture of sodium nitroferricyanide, ammonium sulfate and silica and a liquid including an ammonium hydroxide solution. | 2012-09-13 |
20120231549 | SENSING HYDROXYL RADICALS IN OZONE WASHING SYSTEMS - Implementations and techniques for sensing hydroxyl radicals in ozone washing systems are generally disclosed. | 2012-09-13 |
20120231550 | APPARATUS AND METHOD FOR PRECONCENTRATING AND TRANSFERRING ANALYTES FROM SURFACES AND MEASUREMENT THEREOF USING SPECTROSCOPY - A system and method for capturing a target analyte in advance of performing spectroscopic analysis to determine the existence of the target analyte from a source contacted with a collection substrate. The collection substrate is fabricated of a material selected to have an affinity for the target analyte, sufficiently transparent in a spectral region of interest and capable of immobilizing the target analyte thereon in a manner that limits scattering sufficient to obscure spectral analysis. The collection substrate may be coated with a material selected to react with, bind to, or absorb the target analyte. The method optionally includes the step of transferring the captured target analyte to a second substrate, which may be an optical substrate. The target analyte may be captured to the collection substrate by one or more of wiping, dabbing or swabbing a target analyte carrier with the collection substrate. | 2012-09-13 |
20120231551 | Methods and Compositions to Detect Nucleic Acids in a Biological Sample - Kits, reaction mixtures and methods for separating a target nucleic acid from a sample by using at least one hairpin capture probe oligonucleotide that has the structure 5′-X.sub.n a′ b′ c′ Y.sub.n-3′, wherein X and Y each comprise nucleic acid sequences that can form a double stranded stem portion, one of X or Y is a capture sequence that is a first member of a specific binding pair and the other of X or Y is a terminal sequence of the hairpin capture probe, and a′ b′ c′ comprises a target-complementary sequence flanked by X and Y to thereby form a loop portion of the hairpin, thus forming a capture hybrid that is separated from other sample components before the target nucleic acid is released from the capture support and hybridized to a detection probe that hybridizes specifically to the same sequence that is at least partially hybridized by the a′ b′ c′ portion of the capture probe, thus forming a detectable detection hybrid to indicate the presence of the target nucleic acid in the sample. | 2012-09-13 |
20120231552 | DEVICE FOR A TEST STRIP HOLDER, METHOD AND ARRANGEMENT - For the simplified use of a test strip holder, a device having a holding or positioning device is provided, comprising a mixing chamber, a lid for closing the mixing chamber, and an obvious fluid opening for a fluid passage from the mixing chamber to the test strip holder. This allows for the test strip holder to be inserted into the device in a simple way and a sample can be prepared in the mixing chamber of the device. The device is provided with a mixing chamber that can be closed, wherein a reaction partner, for example a gold conjugate, can be dissolved in the sample for increasing the sensitivity of the test strip. | 2012-09-13 |
20120231553 | SUBSTRATE PROCESSING APPARATUS AND FABRICATION PROCESS OF A SEMICONDUCTOR DEVICE - A substrate processing apparatus includes a processing vessel evacuated by an evacuation system and including therein a stage for holding thereon a substrate to be processed, the processing vessel defining therein a processing space, a processing gas supply path that introduces an etching gas into the processing vessel, a plasma source that forms plasma in the processing space, and a high-frequency source connected to the stage. The processing vessel includes therein a shielding plate dividing the processing space into a first processing space part including a surface of the substrate to be processed and a second processing space part corresponding to a remaining part of the processing space, wherein the shielding plate is formed with an opening having a size larger than a size of the substrate to be processed. | 2012-09-13 |
20120231554 | METHOD AND STRUCTURE FOR REWORKING ANTIREFLECTIVE COATING OVER SEMICONDUCTOR SUBSTRATE - A method and a structure for reworking an antireflective coating (ARC) layer over a semiconductor substrate. The method includes providing a substrate having a material layer, forming a planarization layer on the material layer, forming an organic solvent soluble layer on the planarization layer, forming an ARC layer on the organic solvent soluble layer, forming a pattern in the ARC layer, and removing the organic solvent soluble layer and the ARC layer with an organic solvent while leaving the planarization layer unremoved. The structure includes a substrate having a material layer, a planarization layer on the material layer, an organic solvent soluble layer on the planarization layer, and an ARC layer on the organic solvent soluble layer. | 2012-09-13 |
20120231555 | ADAPTIVE ENDPOINT METHOD FOR PAD LIFE EFFECT ON CHEMICAL MECHANICAL POLISHING - The present disclosure provides a semiconductor manufacturing method. The method includes defining a plurality of time regions of pad life for a polishing pad in a chemical mechanical polishing (CMP) system; assigning a ladder coefficient to the polishing pad according to the plurality of time regions of pad life; defining a plurality of endpoint windows to the plurality of time regions, respectively, according to pad life effect; applying a CMP process to a wafer positioned on the polishing pad; determining a time region of a polishing signal of the wafer based on the ladder coefficient; associating one of the endpoint windows to the polishing signal according to the time region; and ending the CMP process at an endpoint determined by the endpoint window. | 2012-09-13 |