35th week of 2015 patent applcation highlights part 25 |
Patent application number | Title | Published |
20150240194 | Microfluidic System for Reproducing Functional Units of Tissues and Organs In Vitro - A microfluidic system for generating compartmentalized microenvironments of tissues and organs in vitro and for independently perfusing the compartments. A microfluidic device that includes at least a first perfusion path and a second separate perfusion path. The microfluidic device also has a chamber containing a matrix, where the matrix surrounds at least one void whose lumen is in fluidic connection exclusively with the first perfusion path, where the at least one void can be populated with at least one cell type in such way that the cells are in direct contact with the matrix and the matrix is in fluidic connection exclusively with the second separate perfusion path. | 2015-08-27 |
20150240195 | ETHANOL PRODUCTION, USE, AND WASTE RECOVERY USING AQUATIC PLANTS - Embodiments of the present disclosure relate to systems and methods for ethanol production, use, and waste recovery using aquatic plants. In certain embodiments, systems and methods are disclosed for reuse and further processing of waste alcohols, sugars, organic acids and/or byproducts produced by conversion of corn, other grains, or other biomass materials of use in biofuel production methods. | 2015-08-27 |
20150240196 | MULTI-COMPARTMENT DEVICE FOR CELL CLONING AND METHOD OF PERFORMING THE SAME - The problems associated with the traditional methods and devices in the field of cell cloning have been solved by the multi-compartment device and method in the present invention. The device combines the advantages of a traditional petri-dish and a traditional microplate. The multi-compartment device in the present invention comprises sidewalls, which are taller than openings of the multi-compartments. The cells in the suspension flow across the multi-compartments and seed inside the compartments during a plating process. The multi-compartment device in the present invention allows easier plating process, changing conditioned medium, and cell colony detachment and transfer. The multi-compartment device also minimizes the risk of cross-contamination during cloning process and during cell colony transfer. The invention also provides an exemplary method of using the multi-compartment device for cell cloning. In one aspect of the method, the multi-compartment device may be tilted before or after adding the cell suspension during plating process. | 2015-08-27 |
20150240197 | CARBONATION SUMP - Disclosed herein is a pond for cultivating a non-vascular photosynthetic organism in a liquid medium, comprising a sump comprising an inflow slope with an angle of about 20 to about 70 degrees, and optionally an outflow slope of with an angle of about 5 to about 40 degrees. The novel sump design results in good overall carbonation efficiency and does not result in a large loss of biomass of a cultivated organism. | 2015-08-27 |
20150240198 | SYSTEM FOR COOLING PRETREATED BIOMASS PRIOR TO MIXING WITH ENZYMES - A method to treat biomass, e.g., lignocellulosic material, including: performing a pretreatment step on the biomass and discharging the pretreated biomass; in at least a first enzymatic hydrolysis reactor producing a liquefaction material, recirculating at least a portion of the liquefaction material from the at least first reactor to a location upstream of the addition of the enzymes as at least a portion of the coolant for the hot pretreated biomass. | 2015-08-27 |
20150240199 | BALANCED MIXOTROPHY METHODS - Methods of culturing mixotrophic microorganisms in a state of balanced gas composition are disclosed. Parameters of a culture of mixotrophic microorganisms may be controlled to reduce the requirements of externally supplied gases and optimize the production and consumption of gases within the culture by the phototrophic and heterotrophic metabolisms of the mixotrophic microorganisms. | 2015-08-27 |
20150240200 | LACTIC ACID BACTERIUM HAVING IMMUNOMODULATORY AND ANTI-ALLERGIC EFFECTS AND PHARMACEUTICAL COMPOSITION CONTAINING THE SAME - The present invention provides an isolated lactic acid bacterium, | 2015-08-27 |
20150240201 | MODIFIED BCG STRAINS WITH REDUCED OR ELIMINATED ACTIVITY OF LSR2 AND PHARMACEUTICAL COMPOSITION COMPRISING SAME - The invention discloses a live modified | 2015-08-27 |
20150240202 | CULTURE MEDIA, CELL CULTURES AND METHODS OF CULTURING PLURIPOTENT STEM CELLS IN AN UNDIFFERENTIATED STATE - Provided are novel serum-free culture media which comprise basic fibroblast growth factor (bFGF), transforming growth factor beta-3 and ascorbic acid at a concentration of at least about 50 microgram/ml; ascorbic acid at a concentration range of about 400-600 microgram/ml, bFGF at a concentration range of about 50-200 ng/ml, xeno-free serum replacement and a lipid mixture; the IL6RIL6 chimera at a concentration range of about 50-200 picogram per milliliter (pg/ml); or leukemia inhibitory factor (LIF) at a concentration of at least 2000 units/ml; cell cultures comprising same with pluripotent stem cells such as human embryonic stem cells and induced pluripotent stem (iPS) cells, and methods of using same for expanding pluripotent stem cells in an undifferentiated state using two-dimensional or three-dimensional culture systems; and methods of expanding iPS cells in a suspension culture devoid of substrate adherence and cell encapsulation. | 2015-08-27 |
20150240203 | STEM CELL-DERIVED HEPATOCYTES IN CO-CULTURE AND USES THEREOF - The present disclosure provides co-cultures of human pluripotent stem cell derived hepatocytes and at least one non-parenchymal cell population in vitro, methods of preparing the co-cultures and methods of using the co-cultures for high throughput screening and evaluation of drug candidates. The stem cell derived hepatocyte co-culture system provides an in vitro model in which cell viability and relatively mature hepatocyte phenotype of stem cell derived hepatocytes are maintained for extended periods relative to conventional monoculture. | 2015-08-27 |
20150240204 | METHOD FOR POLYCLONAL STIMULATION OF T CELLS BY MOBILE NANOMATRICES - The present invention provides a method for polyclonal stimulation of T cells, the method comprising contacting a population of T cells with a nanomatrix, the nanomatrix comprising a) a matrix of mobile polymer chains, and b) attached to said matrix of mobile polymer chains one or more stimulatory agents which provide activation signals to the T cells; thereby activating and inducing the T cells to proliferate; wherein the nanomatrix is 1 to 500 nm in size. At least one first and one second stimulatory agents are attached to the same or to separate mobile matrices. If the stimulatory agents are attached to separate nanomatrices, fine-tuning of nanomatrices for the stimulation of the T cells is possible. Closed cell culture systems also benefit from this method. | 2015-08-27 |
20150240205 | CONDITIONALLY IMMORTALIZED LONG-TERM STEM CELLS AND METHODS OF MAKING AND USING SUCH CELLS - Disclosed are methods for conditionally immortalizing stem cells, including adult and embryonic stem cells, the cells produced by such methods, therapeutic and laboratory or research methods of using such cells, and methods to identify compounds related to cell differentiation and development or to treat diseases, using such cells. A mouse model of acute myeloid leukemia (AML) and cells and methods related to such mouse model are also described. | 2015-08-27 |
20150240206 | Brown Adipocyte Progenitors in Human Skeletal Muscle - This invention relates to brown adipose tissue (BAT) progenitor cells and methods for isolating BAT progenitor cells from skeletal muscle. BAT progenitor cell surface markers and medium and agents for inducing cell differentiation into brown adipocytes are also provided. In some embodiments, the BAT progenitor cell expresses a first cell surface marker associated with endothelial cells, the first cell surface marker being detectable in an antibody based assay using a first antibody. In addition, the BAT progenitor cell can be substantially free of a second cell surface marker associated with endothelial cells, the second cell surface marker being substantially undetectable in said antibody based assay using a second antibody. The BAT progenitor cell can also be substantially free of additional cell surface markers. | 2015-08-27 |
20150240207 | BONE TISSUE REGENERATION SHEET AND PRODUCTION METHOD THEREOF - To provide a bone tissue regeneration sheet having a cortical bone tissue layer of 200 μm or more in thickness, the bone tissue regeneration sheet is produced by seeding chondroblast or stem cells for differentiating to chondrocyte on one side of a porous bioabsorbable polymer sheet, taking the seeded porous body into a culture medium, applying centrifugal force of 100 to 1000 G to the culture medium for a predetermined time so as to aggregate the chondroblast or the stem cells for differentiating to chondrocyte, culturing the aggregated cells in a culture medium not containing serum but containing one or two or more kinds selected from ascorbic acid, an ascorbic acid derivative, and dexamethasone without applying centrifugal force to form a chondrocyte layer having a thickness of 200 μm or more. | 2015-08-27 |
20150240208 | Chondrogenic Progenitor Cells, Protocol For Derivation Of Cells And Uses Thereof - The present invention provides an isolated population of chondrocyte precursor cells wherein 1% or less of the cells express Oct4, Nanog and/or TRA-1-60, 7% or less of the cells express no collagen II, collagen X, CD105 or Stro-1 and 85% or more of the cells express CBFA1, methods for preparing such cells and uses of chondrocyte cells derived from said precursor cells. | 2015-08-27 |
20150240209 | METHODS FOR THE PREPARATION OF FIBROBLASTS - The invention relates to a process for generating fibroblasts, more particularly, to the culturing of fibroblasts in large numbers and of the heterogenic type. The invention is also directed to the use of fibroblasts in the preparation of heterotypic spheroids and a process for the preparation of such heterotypic spheroids. | 2015-08-27 |
20150240210 | METHOD FOR RAPID MINCING OF ADIPOSE TISSUES TO ISOLATE LIVE CELLS IN VITRO - A method for rapid mincing of adipose tissues to isolate live cells in vitro is disclosed to overcome the drawbacks of a lead procedure of isolating live cells by holding a knife to mince adipose tissues, which can only isolate a small number of live cells and obtain low cell viability. The method includes: providing an adipose tissue; mincing the adipose tissue homogeneously by a mincing device; adding a reagent into the minced adipose tissue to perform hydrolysis; performing centrifuge and isolation; and removing a supernatant to obtain a cell pellet. Therefore, the time of mincing adipose tissues can be shortened, and contaminations caused by repeated use of the knife can be avoided. The method can be used for isolating live cells of adipose tissues to improve the number of live cells per unit weight of adipose tissues without reducing the cell viability. | 2015-08-27 |
20150240211 | Media for Culturing, Preserving, and Administering Regenerative Cells - A culture media, media supplement, or soluble matrix for cryopreservation or enhanced regenerative cell growth in culture and maintenance of multi-lineage differentiation potentiation. The inventive culture media, media supplement, or soluble matrix comprises a GAG composition comprising a sulfated GAG, such as chondroitin sulfate. A soluble matrix, a cell administration package or kit comprising the soluble matrix and a device for cell administration, and a method of use thereof, for administration of regenerative cells for treating a joint disease or other weakened or damaged tissue comprising the specified GAG compositions are further provided. | 2015-08-27 |
20150240212 | SC-BETA CELLS AND COMPOSITIONS AND METHODS FOR GENERATING THE SAME - Disclosed herein are methods, compositions, kits, and agents useful for inducing β cell maturation, and isolated populations of SC-β cells for use in various applications, such as cell therapy. | 2015-08-27 |
20150240213 | COMPOSITIONS AND METHODS FOR PROMOTING THE GENERATION OF DEFINITIVE ENDODERM - Certain embodiments disclosed herein are directed to a method of producing endoderm cells, such as definitive endoderm cells by exposing stem cells such as embryonic stem cells or induced pluripotent stem (iPS) cells to an effective amount of at least one compound described herein to differentiate the stem cells into the endoderm cells such as definitive endoderm cells. Differentiated endoderm cells produced by the methods disclosed herein can be differentiated into pancreatic epithelium, and other endoderm derivatives such as | 2015-08-27 |
20150240214 | INDUCTION OF PLURIPOTENT CELLS - The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (>200 fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, non-viral methods for reprogramming human somatic cells. | 2015-08-27 |
20150240215 | METHOD TO PRODUCE VIRUS IN CULTURED CELLS SUPPLEMENTED WITH ALPHA-KETOGLUTARATE - The present disclosure provides a method to enhance the production of virus in cultured fibroblasts by supplementing the cells with a TCA cycle intermediate, aketoglutarate, or a derivative thereof, wherein virus production is enhanced compared to the same method canied out in the absence of a-ketoglutarate or the derivative thereof. In view of the art, the method provides an unexpected improvement on methods routinely practiced. | 2015-08-27 |
20150240216 | NOVEL GLUCOSE DEHYDROGENASE - The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity and that is suitable for use in SMBG. The present invention provides a flavin-bound glucose dehydrogenase having the following characteristics (1) to (5): (1) Temperature stability: stable at a temperature of 45° C. or lower; (2) stable at a pH range of 4.5 to 7.5; (3) substrate specificity: the reactivity to D-xylose, maltose, or D-galactose is 2% or less, based on the reactivity to D-glucose taken as 100%; (4) optimal activity temperature: 34 to 47° C.; and (5) optimal activity pH: 6.3 to 6.7. | 2015-08-27 |
20150240217 | KETOREDUCTASE-MEDIATED STEREOSELECTIVE ROUTE TO ALPHA CHLOROALCOHOLS - The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of α-chloroalcohols from α-chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides | 2015-08-27 |
20150240218 | Polypeptides Having Peroxygenase Activity - The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from | 2015-08-27 |
20150240219 | METHODS FOR ACCELERATING BONE REPAIR - Vectors, such as retroviral vectors and transposon-based nonviral vectors, are disclosed herein that can be used to target transgene expression to the proliferating periosteal cells and cells in the marrow space after bone fracture. In one embodiment, these vectors include a human Cox-2 gene that is modified to improve mRNA stability and protein translation by truncating the 3′ untranslated region (UTR). In addition, in some embodiments, the native translation signal is replaced with an optimized Kozak sequence. These vectors can be used alone or with vectors expressing BMP2/4, FGF-2, or LMP-1 gene to repair bone fractures and increase prostaglandin secretion. Methods for identifying agents that accelerate bone repair are also disclosed. | 2015-08-27 |
20150240220 | NOVEL UDP-GLYCOSYLTRANSFERASE DERIVED FROM GINSENG AND USE THEREOF - The present invention relates to a novel uridine diphosphate (UDP)-glycosyltransferase, and particularly to a novel UDP-glycosyltransferase derived from | 2015-08-27 |
20150240221 | Recombinant Polymerases for Improved Single Molecule Sequencing - Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template. | 2015-08-27 |
20150240222 | HSP104 VARIANTS AND USES THEREOF - Protein misfolding underpins several fatal neurodegenerative disorders. The application is directed to recombinant Hsp104 proteins comprising missense mutations aimed at correcting these events, and methods for expressing and delivering same. | 2015-08-27 |
20150240223 | METHOD OF USING ALPHA-AMYLASE FROM TALAROMYCES EMERSONII FOR SACCHARIFICATION - A fungal alpha amylase is provided from | 2015-08-27 |
20150240224 | Polypeptides Having Cellulolytic Enhancing Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2015-08-27 |
20150240225 | BIOCHEMICAL STRESS RESISTANT MICROBIAL ORGANISM - It is disclosed a non-naturally occurring microbial organism comprising at least one exogenous nucleic acid encoding an enzyme, or a portion thereof, selected from the group of ammonia lyase. Preferably, the enzyme is PAL3, and the at least one exogenous nucleic acid is obtained from | 2015-08-27 |
20150240226 | NUCLEIC ACIDS AND PROTEINS AND METHODS FOR MAKING AND USING THEM - The invention provides polypeptides, including enzymes, structural proteins and binding proteins, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. Polypeptides, including enzymes and antibodies, and nucleic acids of the invention can be used in industrial, experimental, food and feed processing, nutritional and pharmaceutical applications, e.g., for food and feed supplements, colorants, neutraceuticals, cosmetic and pharmaceutical needs. | 2015-08-27 |
20150240227 | INNOVATIVE DISCOVERY OF THERAPEUTIC, DIAGNOSTIC, AND ANTIBODY COMPOSITIONS RELATED TO PROTEIN FRAGMENTS OF SERYL-TRNA SYNTHETASES - Provided are compositions comprising newly identified protein fragments of aminoacyl-tRNA synthetases, polynucleotides that encode them and complements thereof, related agents, and methods of use thereof in diagnostic, drug discovery, research, and therapeutic applications. | 2015-08-27 |
20150240228 | INNOVATIVE DISCOVERY OF THERAPEUTIC, DIAGNOSTIC, AND ANTIBODY COMPOSITIONS RELATED TO PROTEIN FRAGMENTS OF ISOLEUCYL TRNA SYNTHETASES - Provided are compositions comprising newly identified protein fragments of aminoacyl-tRNA synthetases, polynucleotides that encode them and complements thereof, related agents, and methods of use thereof in diagnostic, drug discovery, research, and therapeutic applications. | 2015-08-27 |
20150240229 | Devices and Methods for Purification of Biological Cells - This invention relates to devices and methods for purifying biological cells. For example, viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a column, including a pipette tip column. Cells can be purified in a sealed chromatographic system. Methods of the invention can aid research, diagnosis and treatment of cancer. | 2015-08-27 |
20150240230 | METHOD FOR PRODUCING AND OBTAINING VARIABLE DOMAINS OF ANTI-DIGOXIN MONOCLONAL ANTIBODY FAB FRAGMENT USING THE MOLECULAR BIOLOGY CLONING TECHNIQUE - Method for producing and obtaining variable domains of anti-digoxin monoclonal antibody Fab fragment using the molecular biology cloning technique, and more precisely the present patent of invention relates to a method for producing and obtaining clones of the anti-digoxin antibody Fab fragment using phage display technology and the characterization of the binding thereof to the antigen; the present innovation pertains to the development of the product for therapeutic use having specific potency and a more precise dose for detoxification of patients undergoing treatment with digoxin. | 2015-08-27 |
20150240231 | ISOLATING TRAFFIC-ENHANCING MUTANTS OF DRUG DELIVERY PROTEIN - The invention relates to methods for isolating traffic-enhancing mutants of drug delivery proteins. In one embodiment, the invention provides a carrier for delivering a therapeutic agent to an organelle, comprising a polypeptide encoded by a mutant penton base gene. In another embodiment, the invention provides a method of enhancing trafficking to a cell by administering a composition comprising a penton base (PB) protein with one or more mutations that enhance cellular entry. | 2015-08-27 |
20150240232 | Deducing Exon Connectivity by RNA-Templated DNA Ligation/Sequencing - A technology is described that is capable of generating high-throughput sequencing (HTS) read length DNA products to accurately and reliably provide exon connectivity information for alternatively spliced isoforms. The method is not limited by the initial size of the isoform as the technology removes the template oligonucleotide sequence and a newly formed full length ligated product provides an HTS-compatible read length sequence that comprises information that corresponds to the consecutive order of the exons in the original template oligonucleotide. | 2015-08-27 |
20150240233 | METHODS FOR IDENTIFYING PLANT PATHOGEN RESISTANCE GENES - Methods are provided for identifying a plant disease resistance (R) gene for a plant disease of interest. The methods involve using bait sequences to select a subgroup of nucleic acids from a group of nucleic acids that are derived from a mutagenized plant that is susceptible to the plant disease of interest but that was produced by mutagenizing a plant that is resistant to the disease of interest. The bait sequences are designed to hybridize to one or more genes from at least one plant R gene family. The methods further involve sequencing the subgroup of nucleic acids to obtain a collection of nucleic acid sequences, comparing such nucleic acid sequences with corresponding sequences of one or more genes that are derived from a resistant plant, and identifying at least one nucleic acid sequence derived from the mutagenized plant that is not identical in sequence to a corresponding sequence from the resistant plant. Further provided are related methods for identifying a gene associated with a phenotypic change for a trait of interest in plants and other organisms. | 2015-08-27 |
20150240234 | METHODS AND COMPOSITIONS FOR THE SPECIFIC INHIBITION OF MYC BY DOUBLE-STRANDED RNA - This invention relates to compounds, compositions, and methods useful for reducing MYC target RNA and protein levels via use of dsRNAs, e.g., Dicer substrate siRNA (DsiRNA) agents. | 2015-08-27 |
20150240235 | METHODS FOR PRODUCING A POPULATION OF PANCREATIC BETA-CELLS - The present invention relates to an in vitro or ex vivo method for producing a population of pancreatic beta-cells, comprising the step of inhibiting the expression or the activity of Arx in a population of pancreatic alpha-cells. The present invention also relates to method for inducing the conversion of pancreatic alpha-cells in pancreatic beta-cells in a patient in need thereof. | 2015-08-27 |
20150240236 | RECOMBINANT RNA PARTICLES AND METHODS OF USE - The present invention provides compositions and methods for the production and delivery of recombinant double-stranded RNA molecules (dsRNA) targeting pathogen sequences, which can be useful as an RNA vaccine. The compositions contain engineered double-stranded RNA particles (dsRPs) that can contain a double-stranded RNA molecule that can be a genome or portion of a genome, which can be enclosed in a capsid or coat protein. The dsRNA molecule also comprises an RNA sub-sequence that binds to a target sequence of a pathogenic organism. The dsRPs can be derived from wild-type viral organisms. The delivery of the dsRPs of the invention to an organism provides a protection to the organism from the pathogen. | 2015-08-27 |
20150240237 | Compositions and Methods for Inhibiting Expression of a Gene from the JC Virus - The invention relates to a double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of a gene from the JC Virus (JC virus genome), comprising an antisense strand having a nucleotide sequence which is less that 30 nucleotides in length, generally 19-25 nucleotides in length, and which is substantially complementary to at least a part of a gene from the JC Virus. The invention also relates to a pharmaceutical composition comprising the dsRNA together with a pharmaceutically acceptable carrier; methods for treating diseases caused by JC virus expression and the expression of a gene from the JC Virus using the pharmaceutical composition; and methods for inhibiting the expression of a gene from the JC Virus in a cell. | 2015-08-27 |
20150240238 | THERAPEUTIC RNA SWITCHES COMPOSITIONS AND METHODS OF USE - The invention provides for therapeutic RNA switches comprising oligonucleotide sequences complementary to a trigger sequence, an antisense strand oligonucleotide, and a sense strand oligonucleotide complementary to a target nucleic acid molecule. | 2015-08-27 |
20150240239 | METHODS AND PHARMACEUTICAL COMPOSITIONS FOR INHIBITING MATRIX METALLOPROTEINASES 1 BY INTERFERENCE RIBONUCLEIC ACID - An interference ribonucleic acid containing specific sequence for inhibiting MMP1, which is composed of 19 to 25 nucleotides, wherein the interference ribonucleic acid has a sequence selected from the group consisted of the sequences as set forth in, or essentially identical to, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, and SEQ ID NO: 10. By means of the interference ribonucleic acid and MMP1 with high specificity binding, gene and protein expression of endogenous MMP1 of cancer cell can be inhibited, and then the interference ribonucleic acid can be used for pharmaceutical composition to prevent or treat cancer metastasis or to improve wrinkle resistance. | 2015-08-27 |
20150240240 | MSF REPROGRAMS MYOFIBROBLASTS TOWARD LACTATE PRODUCTION AND FUEL ANAERBOIC TUMOR GROWTH - Presented herein is a method for treating a subject afflicted with a tumor comprising administering to the subject a therapeutically effective amount of an agent that inhibits the MSF/Cdc42/NFkB cascade in the subject's tumor-associated fibroblasts. | 2015-08-27 |
20150240241 | PAIN TREATMENT USING ERK2 INHIBITORS - This application describes methods and compositions for reducing, inhibiting and/or treating pain that involve use of ERK2 inhibitors. | 2015-08-27 |
20150240242 | Compositions And Methods For Inhibiting Expression Of IKK-B Gene - The invention relates to a double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of the IKK-B gene, comprising an antisense strand having a nucleotide sequence which is less that 30 nucleotides in length, generally 19-25 nucleotides in length, and which is substantially complementary to at least a part of the IKK-B gene. The invention also relates to a pharmaceutical composition comprising the dsRNA together with a pharmaceutically acceptable carrier; methods for treating diseases caused by the expression or activation of the IKK-B gene using the pharmaceutical composition; and methods for inhibiting the expression of the IKK-B gene in a cell. | 2015-08-27 |
20150240243 | Generating Targeted Sequence Diversity in Fusion Proteins - Methods of generating fusion protein variants are provided that comprise introducing sequence diversity at the junction region or regions in the fusion and allows for the generation of variants having a desired activity. Examples include immunoglobulins comprising a domain or polypeptide inserted into, or replacing, a CDR. Also provided are polynucleotides encoding a fusion protein and comprising two or more RSSs, and compositions and host cells comprising same, as well as fusion proteins variants produced by the described methods. | 2015-08-27 |
20150240244 | BIOAVAILABLE DIACYLHYDRAZINE LIGANDS FOR MODULATING THE EXPRESSION OF EXOGENOUS GENES VIA AN ECDYSONE RECEPTOR COMPLEX - The present invention relates to non-steroidal ligands for use in nuclear receptor-based inducible gene expression system, and a method to modulate exogenous gene expression in which an ecdysone receptor complex comprising: a DNA binding domain; a ligand binding domain; a transactivation domain; and a ligand is contacted with a DNA construct comprising: the exogenous gene and a response element; wherein the exogenous gene is under the control of the response element and binding of the DNA binding domain to the response element in the presence of the ligand results in activation or suppression of the gene. | 2015-08-27 |
20150240245 | Human optimized Bacillus anthracis protective antigen - The invention relates to a humanized nucleic acid construct from | 2015-08-27 |
20150240246 | Temperature-dependent insertion of genetic material into genomic DNA - The present invention provides improved methods and reagents for insertion of genetic material into genomic DNA. | 2015-08-27 |
20150240247 | TROPHIC CONVERSION OF PHOTOAUTOTROPHIC BACTERIA FOR IMPROVED DIURNAL PROPERTIES - The present disclosure relates generally to the growth of recombinant bacterial cells of photoautotrophic species under diurnal conditions. In particular, the present disclosure relates to isolated bacterial cells of photoautotrophic species having increased growth under diurnal conditions by expression of a sugar transporter protein and methods of use thereof. | 2015-08-27 |
20150240248 | POLYPEPTIDE EXPRESSION IN CILIATES - This invention is directed to methods for recombinant polypeptide production and, in particular, methods and products for the production of recombinant polypeptides in ciliates. | 2015-08-27 |
20150240249 | EXPANDING THE EUKARYOTIC GENETIC CODE - This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided. | 2015-08-27 |
20150240250 | TRANSFORMANT OF SCHIZOSACCHAROMYCES POMBE MUTANT AND CLONING VECTOR - Provided is a transformant of | 2015-08-27 |
20150240251 | COMPOSITION FOR REGULATING FLOWERING OF A PLANT CONTAINING THE GENE CODING FOR ABI3 PROTEIN - Provided are a composition containing ABI3 gene and a method of delaying flowering of plants using the same. The method has an excellent effect on delaying of flowering of plants. In the previously known method of delaying the flowering of plant, since flowering is delayed by silencing the expression of a gene for promoting flowering, it is difficult to regulate a degree of delaying of flowering. Also, since a gene silencing method such as RNAi is used, it is not easy to produce an actual transgenic plant and thus it is difficult to use for industry. However, ABI3 gene acts in a manner which delays flowering when expression increases, and thus it is easy to regulate the degree of delay in flowering and to produce a transgenic plant. Thus, it is expected that the present disclosure can be effectively applied to increase the productivity of crops by delaying flowering time or blocking flowering in plants of which vegetative tissue or leaves are used. | 2015-08-27 |
20150240252 | DOWN-REGULATION OF AUXIN RESPONSIVE GENES FOR IMPROVED PLANT PERFORMANCE - Methods for modulating plants using optimized ZmSAUR down-regulation constructs are disclosed. Also disclosed are nucleotide sequences, constructs, vectors, and modified plant cells, as well as transgenic plants displaying increased seed and/or biomass yield, improved tolerance to abiotic stress such as drought or high plant density, improved nitrogen utilization efficiency, increased ear tissue growth or kernel number. | 2015-08-27 |
20150240253 | LONG INTERGENIC NON-CODING RNAS IN MAIZE - The invention provides methods and compositions useful in altering gene expression. The invention provides polynucleotide constructs comprising useful for altering gene expression, as well as cells, plants and seeds comprising the polynucleotides. The invention also provides methods for using lincRNAs to alter gene expression. | 2015-08-27 |
20150240254 | Genetic reduction of male fertility in plants - Genetic male sterile plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. | 2015-08-27 |
20150240255 | MODULATION OF SEED VIGOR - The present invention provides a polynucleotide which enables the modulation of the seed vigour, and in particular enhances the seed vigour, and more particularly enables the modification of the speed of germination. A plant seed comprising the said polynucleotide is also provided. A method of producing the plant seed, method for improving the germination and vigour of plant seed, transgenic plant and the use of the polynucleotide of the invention for producing plants growing seeds with improved germination and vigour characteristics are also provided. The invention particularly concerns | 2015-08-27 |
20150240256 | COMPOSITIONS AND METHODS FOR PERONOSPORA RESISTANCE IN SPINACH - The invention provides for spinach plants with broad-spectrum resistance to downy mildew disease and their progeny. Such plants may comprise unique combinations of alleles resulting in the broad-spectrum resistance to downy mildew. In certain aspects, compositions, including distinct polymorphic molecular markers, and methods for producing, using, identifying, selecting, and the like of plants or germplasm with resistance to downy mildew are provided. | 2015-08-27 |
20150240257 | Compositions and Methods Conferring Resistance of Maize to Corn Rootworm II - Methods and compositions for increasing a plant's resistance to an insect pest such as the corn rootworm are provided. Methods are provided for overexpression of Crw2, or variants thereof, in a host plant or plant cell to increase resistance to an insect pest in a plant such as maize. Methods are also provided for identifying variants of Crw2 that when incorporated into a plant via transgenic or traditional breeding means increase resistance to an insect pest in a plant such as maize. Also provided are methods for increasing resistance by overexpressing Crw1 and Crw2. | 2015-08-27 |
20150240258 | Compositions and Methods for Controlling Leptinotarsa - Disclosed herein are methods of controlling insect pests, in particular | 2015-08-27 |
20150240259 | PCP2 Mini-Promoters - Isolated polynucleotides comprising a PCP2 mini-promoter are provided. The mini-promoter may be operably linked to an expressible sequence, e.g. reporter genes, genes encoding a polypeptide of interest, regulatory RNA sequences such as miRNA, siRNA, anti-sense RNA, etc., and the like. In some embodiments a cell comprising a stable integrant of an expression vector is provided, which may be integrated in the genome of the cell. The promoter may also be provided in a vector, for example in combination with an expressible sequence. The polynucleotides find use in a method of expressing a sequence of interest, e.g. for identifying or labeling cells, monitoring or tracking the expression of cells, gene therapy, etc. | 2015-08-27 |
20150240260 | BACULOVIRAL DNA ELEMENTS FOR THE EXPRESSION OF RECOMBINANT PROTEINS IN A HOST INSECT - Reagents and methods are provided that allow for an improved expression of a recombinant protein in an insect, More specifically, the introduction of recombinant DNA elements into an insect larva allows for the increased expression of a recombinant protein, an improvement of the correct folding of said protein and an increase in the survival rate after infection of the insect These recombinant DNA elements can be introduced, for example, into insect larvae via a recombinant baculovirus, which has incorporated said elements. The recombinant DNA elements include nucleic acids encoding transcriptional regulators, such as IE-0 and IE-1, transcriptional, enhancer elements, such as the homologous region (hr) and promoters. | 2015-08-27 |
20150240261 | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX - Isolation or in vitro assembly of the Cas9-crRNA complex of the | 2015-08-27 |
20150240262 | POLYMER SYNTHESIS - A polymer for transfecting a nucleic acid into a cell, the polymer comprising a plurality of polymeric branches, wherein at least one branch comprises at least one disulfide group and at least one vinyl group, which may an unsubstituted vinyl or a functionalized vinyl group. | 2015-08-27 |
20150240263 | METHODS AND COMPOSITIONS FOR NUCLEASE-MEDIATED TARGETED INTEGRATION - Disclosed herein are methods and compositions for targeted, nuclease-mediated insertion of transgene sequences into the genome of a cell. | 2015-08-27 |
20150240264 | METHODS FOR CO-PRODUCTION OF A TERPENE, SUCCINATE AND HYDROGEN - The present disclosure generally relates to microorganisms (e.g., non-naturally occurring microorganisms) that comprise one or more polynucleotides coding for enzymes in a pathway that catalyze a conversion of a carbon source (e.g., a fermentable carbon source) to terpene, succinate, and hydrogen and the use of such microorganisms for the production of terpene, succinate, and hydrogen. | 2015-08-27 |
20150240265 | Processes of Producing a Fermentation Product - The invention relates to a process of process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of an asparaginase and/or an amino acid oxidase; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism. | 2015-08-27 |
20150240266 | DRY GRIND ETHANOL PRODUCTION PROCESS AND SYSTEM WITH FRONT END MILLING METHOD - A dry grind ethanol production process and system with front end milling method is provided for improving alcohol and/or by-product yields, such as oil and/or protein yields. In one example, the process includes grinding corn kernels into particles then mixing the corn particles with a liquid to produce a slurry including oil, protein, starch, fiber, germ, and grit. Thereafter, the slurry is subjected to a front end milling method, which includes separating the slurry into a solids portion, including fiber, grit, and germ, and a liquid portion, including oil, protein, and starch, then milling the separated solids portion to reduce the size of the germ and grit and release bound starch, oil, and protein from the solids portion. The starch is converted to sugar, and alcohol is produced therefrom then recovered. Also, the fiber can be separated and recovered. Oil and protein may be separated and recovered as well. | 2015-08-27 |
20150240267 | PRODUCTION OF RENEWABLE HYDROCARBON COMPOSITIONS - Provided herein are processes and microorganisms which utilize both protein hydrolysates and carbohydrates from biomass feedstocks to produce renewable hydrocarbon compositions. Advantages of the disclosed methods may be recognized in fuel blends comprising such hydrocarbon compositions. | 2015-08-27 |
20150240268 | METHOD FOR PRODUCING ALCOHOLS AND/OR SOLVENTS FROM A BIOMASS, INCLUDING RECIRCULATING AN INTERNAL FLOW INCLUDING ALCOHOLS AND/OR SOLVENTS UPSTREAM FROM OR WITHIN THE PRETREATMENT - The invention relates to a process for the production of alcohols from biomass that comprises a stage in which at least one aqueous internal flow that comprises at least one alcohol that has n carbon atoms, with n between 2 and 5, is recycled, provided that when the alcohol comprises 2 carbon atoms, it is mixed with at least one other alcohol in such a way as to recover a vapor phase effluent that contains alcohols exiting from said pretreatment reactor. | 2015-08-27 |
20150240269 | 3-HYDROXYPROPIONIC ACID-PRODUCING RECOMBINANT MICROORGANISM AND METHOD OF PRODUCING 3-HYDROXYPROPIONIC ACID USING THE SAME - Disclosed is a method of producing 3-hydroxypropionic acid comprising the step of culturing recombinant microorganism comprising a nucleic acid sequence encoding | 2015-08-27 |
20150240270 | PRODUCTION OF ORGANIC ACIDS BY FERMENTATION AT LOW PH - This invention relates to the biosynthesis of organic acids in genetically modified microorganisms. More specifically, this invention provides genetically modified microorganisms that are particularly tolerant to organic acids at low pH and are capable of producing organic acids by fermentation at low pH. | 2015-08-27 |
20150240271 | METHOD FOR THE PRODUCTION OF 3-HYDROXY-3-METHYLBUTYRIC ACID FROM ACETONE AND AN ACTIVATED ACETYL COMPOUND - Described is a method for the production of 3-hydroxy-3-methylbutyric acid by enzyme-catalyzed covalent bond formation between the carbon atom of the oxo group of acetone and the methyl group of a compound which provides an activated acetyl group. Also described are recombinant organisms which produce 3-hydroxy-3-methylbutyric acid, and related compositions and methods. | 2015-08-27 |
20150240272 | LACTATE PRODUCTION PROCESS - A process for producing a compound of formula (I) is provided. The process comprises reacting a mixture comprising a compound of formula (IIA) and a compound of formula (IIB) with an alkyl alcohol in the presence of a | 2015-08-27 |
20150240273 | POLYHYDROXYALKANOATE COPOLYMER COMPOSITIONS AND METHODS OF MAKING THE SAME - A polyhydroxyalkanoate copolymer composition is provided. The composition comprises a plurality of polyhydroxyalkanoate copolymer molecules. The polyhydroxyalkanoate copolymer molecules (i) comprise 3-hydroxybutyrate monomers and 4-hydroxybutyrate monomers, (ii) have a monomeric molar percentage of 4-hydroxybutyrate monomers of 23.5 to 75%, and (iii) have a biobased content of ≧80%. Also provided is a method of making a polyhydroxyalkanoate copolymer composition. The method comprises culturing an organism in the presence of one or more carbon raw materials under conditions under which (a) the one or more carbon raw materials are converted to 3-hydroxybutyryl-CoA and 4-hydroxybutyryl-CoA and (b) the 3-hydroxybutyryl-CoA and the 4-hydroxybutyryl-CoA are polymerized to form the polyhydroxyalkanoate copolymer molecules, thereby forming the composition. The organism has been genetically engineered to comprise particular enzymatic activities, and to not comprise other particular enzymatic activities. The one or more carbon raw materials, taken together, have a biobased content of ≧80%. | 2015-08-27 |
20150240274 | REDUCING INSOLUBLE DEPOSIT FORMATION IN ETHANOL PRODUCTION - The present inventors have surprisingly discovered that phytic acid tenaciously precipitates with soluble metals in food or fuel ethanol-processing fluid, producing insoluble organometallic salt deposit or scale on the processing equipment that must be removed in order to facilitate further ethanol processing. The present invention relates to converting phytic acid salts or phytates to inorganic phosphates to improve metal solubility and reduce deposition within processing equipment. | 2015-08-27 |
20150240275 | ENZYME ASSOCIATED WITH EQUOL SYNTHESIS - An object of the present invention is to provide enzymes associated with equol synthesis, genes coding such enzymes, and a process for producing equol and its intermediates using the enzymes and genes. | 2015-08-27 |
20150240276 | VARIANT LOVD POLYPEPTIDES AND THEIR USES - The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound | 2015-08-27 |
20150240277 | Process for producing monosaccharides - The present invention relates to a process for producing a monosaccharide using a microorganism. The microorganism possesses a glycosyltransferase and a glycosidase which work together to synthesize a desired monosaccharide in free form by using an endogenous provided nucleotide activated monosaccharide, glycosylate a suitable acceptor substrate and release the desired monosaccharide by a hydrolysis reaction. The required acceptor substrate for the reaction is recycled and only needed in catalytic amounts. The monosaccharide is produced in free from and is retrieved from the supernatant of the cultivated microorganism. | 2015-08-27 |
20150240278 | ENZYMATIC HYDROLYSIS OF DISACCHARIDES AND OLIGOSACCHARIDES USING ALPHA-GLUCOSIDASE ENZYMES - A method is disclosed for hydrolyzing an alpha-1,5 glucosyl-fructose linkage in a saccharide (disaccharide or oligosaccharide) such as leucrose. This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase or glucoamylase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,5 glucosyl-fructose linkage of the saccharide. This method is useful for reducing the amount of leucrose in a filtrate isolated from a glucan synthesis reaction, for example. | 2015-08-27 |
20150240279 | ENZYMATIC HYDROLYSIS OF DISACCHARIDES AND OLIGOSACCHARIDES USING ALPHA-GLUCOSIDASE ENZYMES - A method is disclosed for hydrolyzing an alpha-1,3 or alpha-1,6 glucosyl-glucose linkage in a saccharide (disaccharide or oligosaccharide). This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,3 or alpha-1,6 glucosyl-glucose linkage of the saccharide. This method is useful for reducing the amount of oligosaccharides in a filtrate isolated from a glucan synthesis reaction, for example. | 2015-08-27 |
20150240280 | METHODS FOR IN VITRO JOINING AND COMBINATORIAL ASSEMBLY OF NUCLEIC ACID MOLECULES - The present invention relates to methods of joining two or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule of each pair share a region of sequence identity. The method allows the joining of a large number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. Kits for performing the method are also disclosed. The methods of joining DNA molecules may be used to generate combinatorial libraries useful to generate, for example, optimal protein expression through codon optimization, gene optimization, and pathway optimization. | 2015-08-27 |
20150240281 | METHODS OF CELL CULTURE - Polypeptide preparations having target levels of glycans, and methods of producing such polypeptide preparations using DMSO, are described. | 2015-08-27 |
20150240282 | MUTATED CEPHALOSPORIN HYDROXYLASE AND ITS APPLICATION IN DEACETYLCEPHALOSPORANIC ACID SYNTHESIS - A mutant hydroxylase with increased activity and greater substrate specificity towards phenylacetyl-7-ADCA derivatives for the production of phenylacetyl-7-HACA derivatives, which carries one or more amino acid modification at residue positions when compared with certain wild type hydroxylase from certain groups of residues. Also disclosed is a process for the preparation of deacetyl cephalosporanic acid from the corresponding deacetoxy cephalosporanic acid that includes an enzyme of the present invention. Also provided is a method for the production and processing of such enzymes. | 2015-08-27 |
20150240283 | Compound - The present invention concerns rhodamine based fluorescent probes which have use in detecting coagulase-producing bacterial strains. In particular, wherein the bacterial strain is MRSA or MSSA. | 2015-08-27 |
20150240284 | DISPOSABLE CONTAINER FOR BIOBURDEN SAMPLE COLLECTION AND DETECTION - A disposable container for collection of microbe containing fluid samples is useful in a variety of microbial detection methods. One end of the container is provided with a lid comprising fluid flow apertures on one surface and a membrane filter (MF) on an opposed surface. Methods are disclosed wherein the container is disposed in a holding assembly with the lid gripped in a separate platform. The assembly and platform can be displaced relative to each other to separate the lid from the container. | 2015-08-27 |
20150240285 | MUTEINS OF THE PYRROLINE-5-CARBOXYLATE REDUCTASE 1 - The invention relates to muteins of the pyrroline-5-carboxylate reductase 1 (PYCR1), to nucleic acid molecules comprising a nucleotide sequence encoding such muteins, to methods of determining in a subject a predisposition of having an age related disorder associated with PYCR1, to methods of identifying a compound capable of modifying the expression of PYCR1, and methods of treating a subject having an age-related disorder associated with PYCR1. The invention further relates to a genetically modified animal and a method of modifying the expression of the PYCR1 gene in an animal. | 2015-08-27 |
20150240286 | METHODS AND COMPOSITIONS FOR CELL-PROLIFERATION-RELATED DISORDERS - Methods of treating and evaluating subjects having neoactive mutants of IDH (e.g., IDH1 or IDH2). | 2015-08-27 |
20150240287 | METHOD FOR EVALUATING BLOOD COAGULATION REACTION - Various methods for evaluating hemostatic effect and various blood coagulation initiation reagents were studied to construct a method for evaluating blood coagulation reaction mediated by a substance having an activity of substituting for coagulation factor VIII (FVIII). As a result, it was discovered that by using a coagulation initiation reagent containing activated coagulation factor XI (FXIa) and phospholipids, the effect of a substance having an activity of substituting for coagulation factor VIII (FVIII) on blood coagulation reaction can be evaluated using the amount of thrombin generated in the blood sample as an indicator. | 2015-08-27 |
20150240288 | LYOPHILISED DABIGATRAN - The invention relates to a lyophilised form of dabigatran of formula I | 2015-08-27 |
20150240289 | SOLID GEL AMPLIFICATION METHOD AND APPARATUS FOR PLATFORM MOLECULAR DIAGNOSTICS - The present invention provides for a novel system and method for amplification and detection of nucleic acids within a miniaturized device wherein sample administration occurs via capillary forces through a channel created by drying a hydrogel containing all components needed for a cell-free, enzymatic, nucleic-acid amplification system other than the template nucleic acid or precursor thereto, and wherein an aqueous sample is provided to the desiccated hydrogel, and the hydrogel is rehydrated, through capillary forces. | 2015-08-27 |
20150240290 | METHOD FOR EFFICIENT ISOLATION OF MICROBIAL DNA FROM BLOOD - A method allows the simultaneous isolation of bacterial and fungal DNA from blood. The method uses the enzymatic, mechanical and thermal lysis. | 2015-08-27 |
20150240291 | LYOPHILIZATE OF SUBSTANCE-BINDING SOLID-PHASE CARRIER, VESSEL FOR BINDING SUBSTANCE IN SUBSTANCE-CONTAINING LIQUID TO SUBSTANCE-BINDING SOLID-PHASE CARRIER, AND METHOD OF PRODUCING LYOPHILIZATE CONTAINING SUBSTANCE-BINDING SOLID-PHASE CARRIER - A lyophilizate containing a substance-binding solid-phase carrier, which is obtained by lyophilizing the substance-binding solid-phase carrier together with an additive, a vessel for binding a substance in a substance-containing liquid to a substance-binding solid-phase carrier, in which a lyophilizate obtained by lyophilizing the substance-binding solid-phase carrier together with an additive is provided, and a method of producing a lyophilizate containing a substance-binding solid-phase carrier, which includes lyophilizing the substance-binding solid-phase carrier together with an additive, are provided. | 2015-08-27 |
20150240292 | NUCLEIC ACID AMPLIFICATION METHOD, NUCLEIC ACID EXTRACTION DEVICE, NUCLEIC ACID AMPLIFICATION REACTION CARTRIDGE, AND NUCLEIC ACID AMPLIFICATION REACTION KIT - A nucleic acid amplification method includes: adsorbing a nucleic acid on fine particles by mixing an adsorbing liquid and the fine particles with a sample containing the nucleic acid; washing the fine particles having the nucleic acid adsorbed thereon with a first washing liquid; eluting the nucleic acid adsorbed on the fine particles in an eluate by applying ultrasound to the fine particles having the nucleic acid adsorbed thereon; and performing a nucleic acid amplification reaction of the nucleic acid in the eluate. Further provided are a nucleic acid extraction device, a nucleic acid amplification reaction cartridge, and a nucleic acid amplification reaction kit, capable of realizing this method. | 2015-08-27 |
20150240293 | Detection of an Amplification Reaction Product Using pH-sensitive Dyes - Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or PCR amplification reactions to completion using pH-sensitive dyes that are either colored or fluorescent. Compositions are described that include a mixture of a DNA polymerase, deoxyribonucleotide triphosphate and a weak buffer of less than 1 mM Tris or equivalent or no buffer. | 2015-08-27 |