32nd week of 2013 patent applcation highlights part 42 |
Patent application number | Title | Published |
20130203025 | PERFORMANCE AND LEARNING ANALYSIS TOOL - Apparatus for calculating a value relating to the desirability of using a teaching method to train a group of employees. The apparatus may include a receiver configured to receive information relating to a first response selected from a first group of predetermined responses. The first group of predetermined responses may relate to responses to a first question. The apparatus may also include a processor configured to determine, based on the received first response, if the teaching method is a desirable teaching method. In the event that the teaching method is determined to be a desirable teaching method, the processor may assign a first value to the teaching method. In the event that the teaching method is determined to be an undesirable teaching method, the processor may assign a null value to the teaching method. | 2013-08-08 |
20130203026 | System and Method for Virtual Training Environment - Embodiments of the invention are directed to a computer-implemented interactive virtual training system and method for facilitating the virtual interactive training of an employee over a network. The system may include computer storage components for storing training application program modules, a delivery engine for delivering a virtual interactive training environment, the environment including visual and audio components, computer processing components for accessing and executing the training application program modules, and a user input sensing mechanism for accepting and interpreting user input received in the virtual interactive training environment enabling the user to perform training tasks in the interactive virtual training environment. Each training application program module includes training material for one of multiple career paths, wherein the training material is divided into multiple levels, such that successful employee completion of an initial level for one career path allows access to a subsequent level for the career path. | 2013-08-08 |
20130203027 | Adaptive Set Discrimination Procedure - The present disclosure relates to methods and tools to enhance cognition in an individual. The methods involve presenting to the individual multiple sets of stimuli (e.g. two or more sets). Each set of the multiple sets contains two or more background stimuli. Out of the multiple sets presented to the individual, at least one set also contains at least one anomalous stimulus and such set is referred to as the target set. The method then receives an input from the individual that has been instructed to respond to the anomalous stimulus in the target set. | 2013-08-08 |
20130203028 | COORDINATION AND CONTROL TEST DEVICE - A device measuring the control of the individual on the hand and eye coordination and the control of the individual on the hand and arm muscles during the response the individual gives after he/she senses the stimulus visually, in the area of measurement and evaluation of the psychomotor and cognitive processes. | 2013-08-08 |
20130203029 | Virtual Simulator Method and System for Neuromuscular Training and Certification via a Communication Network - A system for neuromuscular training via a communication network, including a computer processor connectable to the communication network, the processor capable of performing operations including: retrieving data representative of a training scenario from a database in response to a user selection on an input device, generating test elements, parameters and controls based on the retrieved data, monitoring use of the input device by the user, performing calculations of a simulated environment in response to the use of the input device by the user and the test elements, parameters and controls, generating real time images replicating the simulated environment of management of the test elements by the user and recording results of the simulation in the database. | 2013-08-08 |
20130203030 | Training Device for Rugby Players - A training device for rugby players, notably for a phase of the game known as the scrum, comprises: a simulation platform of the Stewart platform type, onto which a training yoke is mounted; an electronic device for closed-loop control of said simulation platform; a computer device for controlling the training device. The present invention is mainly applicable to the training, the development and the improvement of rugby players at a high level. | 2013-08-08 |
20130203031 | SYSTEMS AND METHODS FOR OPTIMIZING PARAMETERS OF ORTHOPAEDIC PROCEDURES - Systems and methods for optimizing parameters of an orthopaedic procedure for a particular patient, including parameters relating to the anatomic and biomechanic fit of an implant or implant system implanted into the patient's joint. These systems and methods may utilize patient-specific information gathered pre-operatively in conjunction with optimization algorithms to determine an optimal implant design and an optimal position and orientation for implantation of the implant into the particular patient's joint. | 2013-08-08 |
20130203032 | Methods and Apparatus for Providing Realistic Medical Training - Method and apparatus to provide simulation of a human casualty. In one embodiment an autonomous casualty simulator includes a processing module having a scenario progression controller and a physiological modeling system to receive sensor input and to control effectors. The autonomous casualty simulator can be contained in a nominal human mannequin form. | 2013-08-08 |
20130203033 | EDUCATIONAL GAMING DEVICE FOR LEARNING EVOLUTIONISM - An educational gaming device for learning evolutionism comprises a display device, a storage device and an operation unit. The storage device stores at least one scene and a plurality of corresponding representative creatures. The storage device and the display device are coupled to the operation unit. The operation unit selects at least two creatures from the representative creatures to respectively function as at least one player character and at least one non-player character. The operation unit drives the display device to show the process of interactivity and evolution of the player character and the non-player character in the scene. The game of the present invention features clear rules, instant feedbacks and competitions, and has much more interaction with the users than common instructional activities or common educational animations. | 2013-08-08 |
20130203034 | Computer System and Method for Training, Certifying or Monitoring Human Clinical Raters - A method and system are disclosed for computerized training, monitoring, certification or re-certification of human raters in clinical trials. The invention provides an interactive computerized interview that can be compared scores obtained by human raters to monitor and train clinical raters on a continuing basis in order to reduce rater drift and variance during clinical trials. Remediation can be automatically flagged or provided to improve a deviating rater's performance. Computerized assessment of symptom severity is provided without the need for human clinical raters. The system and method enable screening of prospective patients for inclusion or exclusion from a clinical trial by automatically obtaining computerized ratings of symptom severity. | 2013-08-08 |
20130203035 | LEARNING SUPPORT SYSTEM AND METHOD - The present invention is a learning support system which supports learning on a computer comprising means for reading a question sheet on a computer as an image, means for identifying each problem from the image, means for entering whether correct answer was obtained or not for each problem on the question sheet, and means for identifying components of problems entered as incorrect in the means for entering whether correct answer was obtained or not on a database. | 2013-08-08 |
20130203036 | SYSTEM AND METHOD FOR WIRELESS COMMUNICATION TO PERMIT AUDIENCE PARTICIPATION - User equipment (UE) includes a short-range transceiver configured for communication with a plurality of wireless access points (APs) distributed throughout a venue. The individual UEs can communicate with the venue via the APs. The UEs can be configured to play interactive games with the venue, some of which may be displayed on the large screen in the venue and others that are displayed on the display of the UE. Data may be sent to the UEs individually or in groups. The data may be in the form of advertising, text messaging, images, video, multimedia, or the like. An array of UEs can receive portions of an overall image and function as individual pixels in a large display. | 2013-08-08 |
20130203037 | EXAMINATION MANGEMENT - The present subject matter discloses a method and system ( | 2013-08-08 |
20130203038 | SYSTEM, METHOD AND APPARATUS FOR MANAGING EDUCATION AND TRAINING WORKFLOWS - A method of managing an interaction of a student with a class about a subject, the subject having at least one lesson having at least one topic, the topic having at least one of exposition content, demonstration content and test content, the test content having a query and a scheme for mapping a response to a result, comprising: presenting content to the student and storing the interaction of the student with the content presented. | 2013-08-08 |
20130203039 | LOCATION-BASED INFORMATION FOR EMERGENCY MANAGEMENT - An apparatus for management of information for use in mitigating an emergency includes a data storage element configured to store registrant information obtained from members of a population residing in a particular geographic area and a set of survey questions, and a data storage system, the data storage system being programmed to implement a survey editor for enabling an emergency management official to create a survey for soliciting information from the members for inclusion in the registrant information, the survey editor providing, to the emergency management official, an interface for selecting a subset of questions from the set of questions, the subset of questions being selected from the set of questions based at least in part on the particular geographic area, and a survey portal for receiving, from the member, a response to the survey for integration into the reference data. | 2013-08-08 |
20130203040 | MINIATURE OF WASHING MACHINE FOR EXHIBITION - A miniature of a washing machine for exhibition includes: a casing having an opening at an upper portion thereof; a door for opening and closing the opening; a tub fixedly installed within the casing; a drum rotatably installed within the tub; a driving motor installed at an outer side of the tub; an electric motor unit transferring rotary force of the driving motor to the drum; a tub cover installed at an upper portion of the tub and including a guide unit for guiding water, which is discharged upwardly along an inner wall surface of the tub, to the inner side of the tub when the drum is rotated; and a light emitting unit irradiating light to the interior of the tub. | 2013-08-08 |
20130203041 | PERFUSION SOLUTION - The invention provides a perfusion stock composition, for preserving a donor organ for transplantation, comprising: a source of 60 to 100 mM Na | 2013-08-08 |
20130203042 | METHODS AND SYSTEMS FOR PROCESSING BIOLOGICAL FLUIDS - Methods and container systems for processing biological fluids are disclosed. The container systems include an inner container within an outer container. The inner container wall is made of a porous material of a selected porosity that allows certain components to pass through said porous wall but retains other components. A treating solution is introduced into the chamber of the outer container. | 2013-08-08 |
20130203043 | PORTABLE RAPID DIAGNOSTIC TEST READER - A portable rapid diagnostic test reader system includes a mobile phone having a camera and one or more processors contained within the mobile phone and a modular housing configured to mount to the mobile phone. The modular housing including a receptacle configured to receive a sample tray holding a rapid diagnostic test. At least one illumination source is disposed in the modular housing and located on one side of the rapid diagnostic test. An optical demagnifier is disposed in the modular housing interposed between the rapid diagnostic test and the mobile phone camera. | 2013-08-08 |
20130203044 | SYSTEM, METHOD AND COMPUTER PROGRAM PRODUCT FOR THE ORGANISM-SPECIFIC DIAGNOSIS OF SEPTICEMIA IN INFANTS - A method, system, and computer program product for producing an organism specific diagnosis of septicemia in infants is disclosed. The method involves measuring the levels of one or more biomarkers against redefined threshold values and interpreting these levels to arrive at the diagnosis. Other techniques may introduce a preliminary step of identifying higher risk subjects, as well as the integration of such methods into the final diagnostic methodology. One aspect of a technique of this method may involve measuring one more cytokines to detect specific classes of infective organisms, such as Gram-negative bacteria. | 2013-08-08 |
20130203045 | METHOD FOR DETECTING NUCLEIC ACIDS BASED ON AGGREGATE FORMATION - The invention provides methods to detect or determine the presence or amount of a pathogen, such as a virus or bacterium, in a sample or the amount of cells based on the detection of their genomic DNA. The method employs magnetic substrates and subjects the sample and the magnetic substrate to forms of energy so as to induce aggregate formation and detects the aggregates. | 2013-08-08 |
20130203046 | SYSTEM AND METHOD INCLUDING ANALYTICAL UNITS - Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples. | 2013-08-08 |
20130203047 | Increasing Confidence of Allele Calls with Molecular Counting - Aspects of the present invention include methods and compositions for determining the number of individual polynucleotide molecules originating from the same genomic region of the same original sample that have been sequenced in a particular sequence analysis configuration or process. In these aspects of the invention, a degenerate base region (DBR) is attached to the starting polynucleotide molecules that are subsequently sequenced (e.g., after certain process steps are performed, e.g., amplification and/or enrichment). The number of different DBR sequences present in a sequencing run can be used to determine/estimate the number of different starting polynucleotides that have been sequenced. DBRs can be used to enhance numerous different nucleic acid sequence analysis applications, including allowing higher confidence allele call determinations in genotyping applications. | 2013-08-08 |
20130203048 | Wound Healing Metakaryotic Stem Cells and Methods of Use Thereof - The invention provides methods of identifying wound healing metakaryotic stem cells, identifying molecules to modulate proliferation and/or migration of metakaryotic stem cells and molecules to treat wound healing disorders, such as blood vessel wound healing disorders, including restenosis. The invention also provides methods of diagnosing and treating wound healing disorders, such as blood vessel wound healing disorders and also of treating wounds by the use of metakaryotic stem cell transplant(s). | 2013-08-08 |
20130203049 | METHOD AND APPARATUS FOR CONDUCTING AN ASSAY - The present invention relates to methods and apparatus for conducting nucleic acid sequencing. The method comprises the steps of providing a platform having at least one well for containing at least one support surface, and providing at least one support surface within each well, wherein the support surface is adapted to immobilise a first binding partner or selectively immobilise a second binding partner. The method further comprises the steps of binding or immobilising the first or second binding partner to the support surface and dispensing into each well from a point external of said platform a reagent, wherein after the dispensing step the platform is rotated sufficiently such that any residual or unreacted said reagent is substantially centrifugally removed from each well and/or each support surface, wherein during rotation each support surface is held within each well. The invention also relates to kits and uses of the kits for conducting nucleic acid sequencing. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited to this field. | 2013-08-08 |
20130203050 | HYBRID NANOPORE DEVICE WITH OPTICAL DETECTION AND METHODS OF USING SAME - The invention is directed to a device comprising a protein nanopore immobilized in a lipid layer within an aperture of a solid phase substrate, which provides a stable platform for using first and second members of one or more FRET pairs to generate optical signals as a labeled analyte translocates through the bore of the protein nanopore. In another aspect, the invention is directed to the use of the device to determine the nucleotide sequence of a polynucleotide analyte. | 2013-08-08 |
20130203051 | Methods for prenatal diagnosis of chromosomal abnormalities - Chromosomal abnormalities are responsible for a significant number of birth defects, including mental retardation. The present invention is related to methods for non-invasive and rapid, prenatal diagnosis of chromosomal abnormalities based on analysis of a maternal blood sample. The invention exploits the differences in DNA between the mother and fetus, for instance differences in their methylation states, as a means to enrich for fetal DNA in maternal plasma sample. The methods described herein can be used to detect chromosomal DNA deletions and duplications. In a preferred embodiment, the methods are used to diagnose chromosomal aneuploidy and related disorders, such as Down's and Turner's Syndrome. | 2013-08-08 |
20130203052 | FREE CIRCULATING DNA BIO-MARKERS AND THEIR APPLICATIONS - This invention relates generally to methods for detecting cell damage as a consequence of pathophysiological or traumatic insults such as in a nuclear accident, bioterror attack, tumorigenesis, infections or in individuals with cardiovascular disease. | 2013-08-08 |
20130203053 | METHODS FOR IMPROVING INFLAMMATORY BOWEL DISEASE DIAGNOSIS - The present invention provides methods and systems to diagnose the ulcerative colitis (UC) subtype of inflammatory bowel disease (IBD) by detecting the presence or absence of one or more variant alleles in the GLI1, MDR1, and/or ATG16L1 genes. Advantageously, with the present invention, it is possible to provide a diagnosis of UC and to differentiate between UC and Crohn's disease (CD) with increased accuracy. | 2013-08-08 |
20130203054 | ANTIVIRAL TREATMENT SUSCEPTIBILITY GENE AND USES THEREOF - The present invention relates to an in vitro method for determining the likelyhood for a patient affected with a viral infection to respond to a treatment with an antiviral agent and/or an interferon, which method comprises determining alteration in CTGF gene locus or in CTGF expression or in CTGF activity in a biological sample of the patient. | 2013-08-08 |
20130203055 | METHODS AND KITS FOR IN SITU DETECTION OF NUCLEOTIDE SEQUENCES - The present invention relates to in situ hybridization methods comprising a room temperature hybridization step for detecting a target nucleic acid in a biological sample. The invention further relates to kits for performing such methods. | 2013-08-08 |
20130203056 | Detection of methicillin-resistant Staphylococcus aureus - The present invention provides improved tests for the detection of methicillin-resistant | 2013-08-08 |
20130203057 | STEP-WISE DETECTION OF MULTIPLE TARGET SEQUENCES IN ISOTHERMAL NUCLEIC ACID AMPLIFICATION REACTIONS - Compositions and methods useful in nucleic acid assays are provided. The invention permits detection of multiple target sequences and control nucleic acids using isothermal nucleic acid amplification methods and subsequent detection of amplification products at different temperature steps by at least two probes with different annealing temperatures. This method can be used in isothermal nucleic acid amplification reactions to detect multiple targets of interest. In a particular example, cycling hybridization probes with different spectral and hybridization temperatures are used to detect different target sequences. Probes become fluorescent when they are cleaved by a thermostable ribonuclease, which only acts when the probes are hybridized to their respective templates. | 2013-08-08 |
20130203058 | COMPOSITE ASSAY FOR DETECTING A CLINICAL CONDITION - The invention generally relates to methods for screening patients for one or more clinical conditions using a composite assay. According to certain aspects, methods of the invention involve isolating at least one nucleic acid from a biological sample obtained from the subject, detecting at least one sequence mutation and a chromosomal abnormality in the at least one nucleic acid in a single assay format, and identifying a clinical condition in said subject when both the sequence mutation and the chromosomal abnormality are present. | 2013-08-08 |
20130203059 | Method for Diagnosis of Bladder Cancer and Related Kits - The invention refers to a novel molecular biomarker, namely PTPD1, that is markedly increased in human bladder cancers. PTPD1 expression positively correlated with the grading and invasiveness potential of these tumors. PTPD1 can be detected at high levels in exfoliated bladder cells isolated from urine of bladder cancer patients, while no PTPD1 signal was evident in normal exfoliated bladder cells. Thus, PTPD1 detection in urine samples may represent a novel and reliable marker for non-invasive diagnosis of aggressive bladder cancer. | 2013-08-08 |
20130203060 | MAMMALIAN GENES; RELATED REAGENTS - Nucleic acids encoding a new family of small cysteine rich soluble proteins, from a mammal, reagents related thereto, including specific antibodies, and purified proteins are described. Methods of using said reagents and related diagnostic kits are also provided. | 2013-08-08 |
20130203061 | METHODS AND SYSTEMS FOR ISOLATING, STORING, AND ANALYZING VESICLES - Provided herein are methods and systems for isolating, storing, and analyzing a vesicle from a sample The vesicle can be isolated using one or more lectins that bind to a vesicle One or more additional binding agents, such as a non lectin binding agent can also be used to isolate or analyze a vesicle | 2013-08-08 |
20130203062 | METHOD FOR DETECTING RHABDOMYOSARCOMA USING SAMPLE DERIVED FROM BODY FLUID - The present invention provides a method for detecting rhabdomyosarcoma comprising evaluating expression of at least one kind of miRNA selected from the group consisting of hsa-miR-1, hsa-miR-133a, hsa-miR-133b, and hsa-miR-206 in a sample. derived from body fluid. | 2013-08-08 |
20130203063 | Procedure for Structural Characterization of a Recombinant Polyclonal Protein or a Polyclonal Cell Line - The present invention provides a structural characterization platform that can be used to assess the stability of a polyclonal cell line during production, as well as batch-to-batch consistency of the final polyclonal products. The structural characterization platform is based on genetic analyses as well as protein characterization techniques that alone or in combination provides the necessary information to characterize the polyclonal cell line and final products. The collection of different homologous proteins to be analyzed with the platform techniques is for example a recombinant polyclonal antibody or a mixture of monoclonal antibodies. | 2013-08-08 |
20130203064 | NOVEL ALTERNATIVE SPLICING VARIANT OF OATP1B3 mRNA - [PROBLEM TO BE SOLVED] | 2013-08-08 |
20130203065 | METHOD FOR ELECTROCHEMICAL DETECTION OF BINDING REACTIONS - A method for performing homogeneous immunoassay formats with electrochemical detection in solution includes combining two different conjugates as reagents with a sample or a sample/buffer mixture, one conjugate including a redox marker and an analyte molecule and the second conjugate including an anti-redox marker antibody or a specifically binding fragment thereof and a molecule specifically binding the analyte. | 2013-08-08 |
20130203066 | METHOD FOR DETECTING INVASIVE MICROVESCLES DERIVED FROM TUMOR CELLS - The present application relates to the isolation and analysis of populations of microvesicles and the identification of invasive microvesicles in the populations such as populations of microvesicles that are shed by tumor cells. Invasive microvesicles from tumor cells contain a variety of specific proteins, such as ARF6. | 2013-08-08 |
20130203067 | DETECTION OF INTRACELLULAR BINDING EVENTS BY MEASURING PROTEIN ABUNDANCE - Methods and compositions are provided to measure the binding of a test compound to a target peptide by measuring the effect of the compound on the abundance of the target peptide inside a cell. The target peptide may bind the test compound at an active site or an allosteric site, and it has been found that such binding may stabilize the target peptide against cellular degradation. The target peptide will preferably comprise a destabilizing mutation which shortens the half life of the target peptide within the cell, typically a mammalian cell. Test compounds, including small molecules, have been found to stabilize target peptides. Also provided are systems and kits for use in practicing the methods. | 2013-08-08 |
20130203068 | DUAL-ACCEPTOR TIME-RESOLVED-FRET - Methods for determining the presence or absence of a plurality of analytes in a sample with dual-acceptor time-resolved fluorescence resonance energy transfer are provided. | 2013-08-08 |
20130203069 | Dynamic Assay for Maintenance and Disruption of Tissue Level Organization and Architecture - A micropatterning approach, systems and methods that confine cells to a specified geometry combined with an algorithm to quantify changes of cellular distribution over time to measure the ability of different cell types to self-organize relative to each other and detect loss of cellular self-organization. | 2013-08-08 |
20130203070 | METHODS OF USING IL-31 ANTAGONISTS IN DISEASES MEDIATED BY CUTANEOUS LYMPHOCYTE ANTIGEN POSITIVE T CELLS - The present invention relates to predicting therapeutic response of treating patients suffering from itching and puritis mediated by cutaneous lymphocyte antigen positive T cells in atopic dermatitis. The invention also includes methods of predicting a therapeutically responsive patient population. | 2013-08-08 |
20130203071 | PEPTIDE APTAMERS FOR MANIPULATING PROTEIN FUNCTION - Peptide aptamers and the methods to produce cassettes including the aptamers and manipulating them, are described. The peptide aptamer cassettes are useful to, e.g., inhibit protein function such as proteins necessary for the transformation of plants, or to replicate cells. | 2013-08-08 |
20130203072 | APPARATUS FOR PROCESSING BIOLOGICAL SAMPLES AND METHOD THEREOF - The present invention provides devices, apparatuses and methods for automated processing of biological samples. This invention provides automated devices and automated methods of sequentially treating a biological sample with processing fluids in more than one washing basin. In some embodiments, the invention provides automated devices and automated methods of western blot processing. In some embodiments, the invention provides automated devices and automated methods of Southern blot processing. In some embodiments, the invention provides automated devices and automated methods of northern blot processing. In some embodiments, the invention provides automated devices and automated methods of staining biomolecules on solid supports. In some embodiments, the invention provides automated devices and automated methods of nucleic acid separation and isolation. | 2013-08-08 |
20130203073 | ASSAY FOR ANALYTES BASED ON AGGREGATION - The present invention relates to compositions, assay devices, kits and methods for detecting the presence, amount and/or activity of an analyte in a sample. In particular, the present invention relates to the detection of enzymes. The present invention also relates to methods of diagnosing diseases associated with dysregulation of enzymes, screening for modulators of enzymatic activity, candidate antimicrobial peptides and toxins. | 2013-08-08 |
20130203074 | METHODS AND COMPOSITIONS FOR DIAGNOSIS AND PROGNOSIS OF RENAL INJURY AND RENAL FAILURE - The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using a one or more assays configured to detect a kidney injury marker selected from the group consisting of Tumor necrosis factor receptor superfamily member 8, Alpha-Fetoprotein, Thyroxine-binding globulin, Prostate-specific antigen (free form), Apolipoprotein A Apolipoprotein E, Thyrotropin subunit beta, Platelet-derived growth factor B/B dimer, C-C motif chemokine 7, C-C motif chemokine 26, Complement C4-B, Corticotropin, Interferon alpha-2, Interleukin-4 receptor alpha chain, Insulin-like growth factor-binding protein 4, Insulin-like growth factor-binding protein 5, Interleukin 21, Interleukin 23 alpha subunit, Interleukin-28A, Interleukin-33, Lutropin subunit beta, Matrix Metalloproteinase-1, Neural cell adhesion molecule 1, Pigment epithelium-derived factor, Vascular endothelial growth factor receptor 2, Vascular endothelial growth factor receptor 3, and IgG4 as diagnostic and prognostic biomarkers in renal injuries. | 2013-08-08 |
20130203075 | METHOD FOR DETECTING ANTI-DRUG ANTIBODIES - The present inventions relates to the monitoring or assaying of anti-bio-agent antibodies, such as anti-drug antibodies (ADA) in patients who may have developed an antibody response in treatments with immunoglobulin bio-agents. | 2013-08-08 |
20130203076 | COMPOSITIONS AND METHODS FOR DETECTION OF METHADONE METABOLITE - Methods and reagents are disclosed for conducting assays for EDDP. The reagents include a moiety selected from the group consisting of poly(amino acid) label moieties, non-poly(amino acid) label moieties, poly(amino acid) immunogenic carriers, non-poly(amino acid) immunogenic carriers, non-label poly(amino acid) moieties, and non-immunogenic carrier poly(amino acid) moieties linked to 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine at the 3-position of one of the phenyl rings. Antibodies produced from immunogenic EDDP conjugates and labeled EDDP conjugates are employed in assays for determining the presence and/or amount of EDDP in samples suspected of containing EDDP. | 2013-08-08 |
20130203078 | NOVEL ULTRASENSITIVE CELL BASED SENSORS AND USES THEREOF - The present invention relates to a novel cell based sensor useful for drug discovery that comprises a cell line with professional regulated exocytosis of secretory granules transfected with a protease as a reporter polypeptide stored in the regulated secretory granules of the cell line with professional regulated exocytosis and having either an endogenous or a heterologous molecule as a modulator of regulated secretory granules exocytosis, such said granule stored protease reporter having at least: a high resistance to conditions already present inside the granules such as low pH and proteolysis by other proteases; enzymatic activity after exocytosis; a highly specific cleavage sequence; a very low level of secretion under unstimulated or basal conditions; and a high signal to background activity in a media compatible with cell culture viability and granule exocytosis for a high throughput robust and sensitive detection. | 2013-08-08 |
20130203079 | ANTIBODIES FOR THE DETECTION OF INTEGRIN COMPLEXES IN FFPE MATERIAL - The invention relates to antibodies that are capable to bind the extracellular domain of integrin. Another object of the invention concerns the use of said antibodies for detecting integrins in archival formalin fixed paraffin embedded (FFPE) tissue. The invention also relates to methods for preparing monoclonal rabbit antibodies, wherein the immunogen is an insect expression culture-derived recombinant extracellular integrin domain, and another method for screening anti-integrin antibodies that discriminate between closest integrin homologues and that are especially suited for immunohistochemistry in FFPE material. | 2013-08-08 |
20130203080 | NEW RECEPTOR BINDING LIGANDS, THEIR USE IN THE DETECTION OF CELLS WITH BIOLOGICAL INTEREST - A method for the identification and quantification of the expression of membrane receptors present on the surface of target cells using at least two soluble receptor binding ligands derived from the soluble part of the glycoprotein of an enveloped virus that interacts with a cellular cognate receptor, the receptor binding ligand containing a part or the totality of one of the receptor binding domains (RBD) of the glycoprotein, and the soluble receptor binding ligand being liable to interact with the at least one membrane receptor of a target cell, for the identification and quantification of the expression of membrane receptors present on the surface of target cells, the identification and quantification taking place at a given time or during a given time interval, and allowing the determination of a physiological state of the target cell. | 2013-08-08 |
20130203081 | TUMOR CELL-DERIVED MICROVESICLES - The present invention relates to a method for diagnosis of cancer and for monitoring the progression of cancer and/or the therapeutic efficacy of an anti-cancer treatment in a sample of a subject by detecting oncogenic and cancer related proteins in microvesicles, and to the use of an agent blocking exchange of microvesicles for treating cancer. | 2013-08-08 |
20130203082 | METHOD FOR DETERMINING CANCER ONSET OR CANCER ONSET RISK - A highly accurate and quantitative method for determining cancer onset or cancer onset risk by a quantitative tissue staining method in biological tissues using an antibody capable of recognizing a cancer growth regulatory factor or cancer metastasis regulatory factor such as PAR1 antibody, which inhibits the cancer cell mobility and infiltration is provided. Cancer onset or cancer onset risk is determined using the tissue staining method comprising the steps of: labeling an antibody which recognizes a cancer growth regulatory factor or cancer metastasis regulatory factor with a fluorescent material, and contacting the fluorescent-labeled antibody with a tissue sample; irradiating a tissue site in contact with the antibody with excitation light to acquire a fluorescence image; acquiring an autofluorescence image in a vicinity region of a short wavelength side or long wavelength side of an acquisition region of fluorescence wavelength emitted by the fluorescent material, in the same field of vision and in the same focal point as those of the fluorescence image; acquiring a corrected fluorescence image by image processing to eliminate a fluorescent brightness of the autofluorescence image from the fluorescent brightness of the fluorescence image; counting the number of cells at the tissue site in contact with the antibody; measuring a mean fluorescent brightness of a single fluorescent particle; and calculating the number of fluorescent particles per cell. | 2013-08-08 |
20130203083 | SERUM CLUSTERIN LEVELS IN SYSTEMIC AMYLOIDOSIS FEATURING CARDIOMYOPATHY - The present invention relates generally to diagnostic methods, systems, assays and kits for identification of subjects with cardiac amyloid deposits, where a low level of clusterin protein in a peripheral fluid sample, e.g., a serum sample from the subject, indicates the subject likely has cardiac amyloid deposits. Other aspects relate to methods of treatment of diseases or disorders characterized by cardiac amyloid deposits and transthyretin (TTR) amyloidosis, and more particularly to methods of treatment of cardiac-related amyloidosis and cardiac amyloid deposits in subjects with familial transthyretin (TTR), senile systemic amyloidosis (SSA), or familial amyloidodic polyneuropathy (FAP), or immunoglobulin light chain (AL) amyloidosis. Other aspects relate to methods and compositions comprising clusterin (CLU) or a clusterin agent (e.g. an agonist of clusterin activity or a biologically active fragment or derivative thereof), and their use in methods to treat a disease or disorder characterized by transthyretin (TTR) amyloidosis, e.g. senile systemic amyloidosis (SSA) or familial amyloidodic polyneuropathy (FAP), and their use in methods to treat amyloidotic cardiomyopathy associated with transthyretin (TTR) amyloidosis. | 2013-08-08 |
20130203084 | SPECIFIC DETECTION OF HUMAN CHORIONIC GONADOTROPIN BETA SUBUNIT TYPE II PRODUCED BY TROPHOBLASTIC AND NEOPLASTIC CELLS - The present invention provides a novel method to distinguish between HCG β type I and type II gene expression using specific antibody. The specific recognition of HCGβ encoded by type II genes and expressed by trophoblastic and neoplastic cells might improve the clinical usefulness of assays aimed at either diagnosing tumors or screening Down's syndrome. The present invention also provides a diagnostic kit for determining the amount of HCGβ type II in a biological sample. The present invention additionally provides process of preparation and screening hybridoma capable of specifically recognizing HCGβ type II and recombinant antibody thereof. Finally, the present invention provides methods for detecting trophoblast or non-trophoblast malignancy in a sample. | 2013-08-08 |
20130203085 | ANTIGENIC STRUCTURE AND USES THEREOF FOR SCREENING TRYPANOSOMIASES IN HUMANS AND ANIMALS - The invention relates to an antigenic structure that contains a tryptophan epitope, characterised in that said structure is made up of a tryptophan pattern W or a peptide of 3 or 4 amino acids comprising a pattern W, coupled with glutaraldehyde. The invention can he used for screening trypanosomiasis in humans or animals. | 2013-08-08 |
20130203086 | MICROFLUIDIC DEVICE FOR GENERATING NEURAL CELLS TO SIMULATE POST-STROKE CONDITIONS - This application provides devices for modeling ischemic stroke conditions. The devices can be used to culture neurons and to subject a first population of the neurons to low-oxygen conditions and a second population of neurons to normoxic conditions. The neurons are cultured on a porous barrier, and on the other side of the barrier run one or more fluid-filled channels. By flowing fluid with different oxygen levels through the channels, one can deliver desired oxygen concentrations to the cells nearest those channels. | 2013-08-08 |
20130203087 | DIAGNOSTIC REAGENTS - There is provided a diagnostic reagent useful to determine whether an animal has a tuberculosis infection or has been exposed to a tuberculosis agent, for example a | 2013-08-08 |
20130203088 | METHODS AND MEANS FOR DIAGNOSING SPONDYLARTHRITIS USING AUTOANTIBODY MARKERS - The present invention relates generally to methods for diagnosing the presence or the risk of development or the therapy control of spondyloarthritis (Spa), in particular, of ankylosing spondylitis (AS) and undifferentiated spondyloarthritis in a subject, in particular in mammals. In addition, the present invention relates to test kits for use in the diagnosis of the presence or the risk of development, or for the therapy control of Spa, like AS and undifferentiated spondyloarthritis, in a subject. In particular, the present invention relates to a method for diagnosing the presence or the risk of development, or for the therapy control of Spa, like AS and undifferentiated spondyloarthritis, in a subject analysing for the presence of autoantibodies against CD74 and/or IKBKB in a subject. The presence of autoantibodies against CD74 and/or IKBKB is indicative for the presence or the risk of development, or for the therapy control of Spa, like AS and undifferentiated spondyloarthritis. In particular, detection of the presence of autoantibodies against CD74 and/or IKBKB allows early diagnosis of Spa, in particular, AS and undifferentiated spondyloarthritis. | 2013-08-08 |
20130203089 | Filtered Adapter for Pipettors - The present invention relates to an adapter containing a filter that can be used with pipetting devices, particularly robotic pipetting devices, to efficiently connect the pipetting device to pipette tips with minimal distortion of the pipette tip. The present invention also relates to a process and a system of using an adapter containing a filter that can be used with pipetting devices, particularly robotic pipetting devices, to assemble the adapter together with an unfiltered pipette tip on the site of application. The adapter and pipette tip are each stackable for saving space at the work station. | 2013-08-08 |
20130203090 | SYSTEMS AND METHODS FOR DETERMINING ANTIBODY-MEDIATED RISK INDEX - Disclosed herein are systems and methods for assessing the risk of risk of hemolytic disease of the fetus or neonate, neonatal alloimmune thrombocytopenic purpura, or transfusion-associated lung injury in a patient or transfusion recipient. | 2013-08-08 |
20130203091 | MOESIN FRAGMENTS ASSOCIATED WITH APLASTIC ANEMIA - The present application provides compositions and methods useful for detecting and monitoring acquired aplastic anemia. | 2013-08-08 |
20130203092 | Lectin Assay for Assessing Glycoforms as an Early Marker in Disease - The present invention provides methods, compositions, apparatus, and kits for assessing glycoforms of proteins as a biomarker for disease. A purified recombinant form of lectin is used as a detector molecule to specifically label target sugars expressed in disease states. The high affinity of recombinant lectin allows for automation of assays that would be used in the diagnosis of diseases such as, but not limited to, cancer or liver disease. | 2013-08-08 |
20130203093 | FLAVIN-BINDING GLUCOSE DEHYDROGENASE - The invention provides a flavin-binding glucose dehydrogenase exhibiting reduced fluctuation of activity depending on temperature environment, and a method for measuring glucose concentration using the flavin-binding glucose dehydrogenase. The flavin-binding glucose dehydrogenase has the following properties (1) to (3): (1) activity: which exhibits glucose dehydrogenase activity in the presence of an electron acceptor; (2) substrate specificity: which exhibits an activity of 10% or less against maltose, D-galactose, D-fructose, sorbitol, lactose and sucrose when the activity against D-glucose is defined as 100%; and (3) temperature characteristics: which exhibits lower fluctuation of activity in a wide temperature range of 10 to 50° C. | 2013-08-08 |
20130203094 | Devices and Formulations for Detecting, Screening and Monitoring Levels of Certain Constituents in Bodily Fluids and Method - A device and method are disclosed for use in the conduct of a non-invasive analysis of a bodily fluid to determine the presence and level of a certain constituent carried by the bodily fluid, which analysis utilizes an indicator formulation that changes color in response to exposure to the constituent to provide a visible indication of the presence and level of the constituent carried by the bodily fluid. A stabilizing formulation is carried in a vessel for mixing with a sample of the bodily fluid to be analyzed. The stabilizing formulation includes a first component for promoting formation of a film, a second component for adjusting the pH of the sample and inhibiting microbial digestion of the certain constituent carried by the bodily fluid, and a third component for reducing interference of ascorbic acid present in the bodily fluid. Where the certain constituent is glucose, the stabilizing formulation includes a microbial digestion inhibitor. | 2013-08-08 |
20130203095 | METHOD OF SCREENING PLACENTAL PROTEINS RESPONSIBLE FOR PATHOPHYSIOLOGY OF PREECLAMPSIA, AND MARKER FOR EARLY DIAGNOSIS AND PREDICTION OF PREECLAMPSIA - The present invention relates to a method of screening placental proteins responsible for pathophysiology of preeclampsia, and a marker for early diagnosis and prediction of preeclampsia. In accordance with one aspect of the present invention, there is provided a method of screening placental proteins responsible for pathophysiology of preeclampsia by 2D E-proteomics analysis, comprising: isolating placental proteins from a placental tissue; separating the isolated proteins two-dimensionally through 2D electrophoresis; and comparing and analyzing the separated proteins based on scanned gel images and differences in the images between normal placental proteins and preeclamptic placental proteins, wherein the comparison and analysis of the placental proteins based on the scanned gel images and differences in the images are accomplished by selecting proteins with differences of 140% or more between two placentas. | 2013-08-08 |
20130203096 | Selected Reaction Monitoring Assays - Provided herein are methods for developing selected reaction monitoring mass spectrometry (LC-SRM-MS) assays. | 2013-08-08 |
20130203097 | SUBSTRATES THE FLUORESCENCE OF WHICH IS SUPPRESSED, PREPARATION THEREOF, AND USE THEREOF FOR IDENTIFYING, DETECTING, AND ASSAYING LEGIONELLA PNEUMOPHILIA - The present disclosure includes the use of an R—(X) | 2013-08-08 |
20130203098 | Enzyme Sensors, Methods for Preparing and Using Such Sensors, and Methods of Detecting Protease Activity - Embodiments of the present disclosure provide for enzyme sensors, protease sensors, methods for producing and using the enzyme and protease sensors, methods of detecting and/or measuring protease activity, methods for characterizing protease cellular activity, fusion proteins, polynucleotides, and vectors corresponding to the enzyme and protease sensors, kits, and the like. | 2013-08-08 |
20130203099 | THYROGLOBULIN QUANTITATION BY MASS SPECTROMETRY - Provided are methods for determining the amount of thyroglobulin in a sample using various purification steps followed by mass spectrometry. The methods generally involve purifying thyroglobulin in a test sample, digesting thyroglobulin to form peptide T129, purifying peptide T129, ionizing peptide T129, detecting the amount of peptide T129 ion generated, and relating the amount of peptide T129 ion to the amount of thyroglobulin originally present in the sample. | 2013-08-08 |
20130203100 | OPPOSABLES AND AUTOMATED SPECIMEN PROCESSING SYSTEMS WITH OPPOSABLES - A specimen processing system is capable of processing specimens carried on slides. The specimen processing system can sequentially deliver slides and opposables to specimen processing stations. The specimen processing stations can use the opposables to apply a series of liquids to the specimens. The applied liquid can be moved along the slide using capillary action while the specimen processing stations control the processing temperatures. The applied liquid can be in a fluid-carrying gap. The opposable can contact the slide to vary a cross section of the fluid-carrying gap. | 2013-08-08 |
20130203101 | METHOD AND SYSTEM FOR PURIFYING AND QUANTITATING PROTEINS USING HEME FUSION TAGS - A method is provided for purifying a protein comprising the steps of providing a heme tag with an open coordination site and tagging the recombinant protein of interest with the heme tag. A resin framework is used, wherein a base that binds to heme is immobilized to the resin, and the open coordination site of the heme tag is capable of reversibly binding to the base immobilized to the resin. The tagged protein is reversibly bound to the resin, then eluted from the resin and quantified. The method enables the tagged protein to be tracked during protein expression or purification, and can be used to identify secretion of a protein to the periplasm or to tag proteins in the cytoplasm. | 2013-08-08 |
20130203102 | Device and Method for Transporting and Preparing Cellular Material - Embodiments of the invention relate to a device for transporting and preparing cellular material in a fluid for subsequent diagnostic purposes, comprising a cylinder having a fluid-tight screw-on cap and a fluid-tight base part that can be screwed off of the cylinder. The cap can be screwed onto the base part in a fluid-tight matter. The device provides for introducing cellular material to be diagnosed into a fluid in the cylinder, closing the cylinder by a cap for transport, removing the cap, introducing a filter device and concentrating the cellular material on a filter surface, after which the fluid level is at that part of the base part below the connection to the cylinder, disposing of the filtrate, removing the base part, pressing the filter surface onto an object carrier for a cell analysis, and closing the base part by the cap for further transport. | 2013-08-08 |
20130203103 | INFORMATION NOTIFICATION SAMPLE PROCESSING SYSTEM AND METHODS OF BIOLOGICAL SLIDE PROCESSING - A sample processing system that may be automated and methods are disclosed where samples are arranged on a carrier element and a process operation control system automatically processes the samples perhaps robotically with an operationally-influential exteriorly-consequential information monitor or a data capture element. Significant process details as well as operationally-influential exteriorly-consequential information may be monitored and an automatic notice element may cause notification of a person at some display that may be remote. Various people may be notified, such as an administrator, a supplier, or a manufacturer of an opportunity for some action such as reagent reordering or the like. A simulated motion display may be included to “watch” simulated operation in real time or long after completion of the actual processing. | 2013-08-08 |
20130203104 | FLUORESCENT PROTEINS WITH INCREASED ACTIVITY IN CELLS - The present invention relates to fluorescent proteins, in particular green fluorescent proteins (GFPs), with increased activity in cells, and thus increased signal strength. A further aspect of the present invention relates to the use of peptides for increasing the expression and/or stability of a protein in a cell. | 2013-08-08 |
20130203105 | METHOD FOR PRE-TREATING SPECIMEN AND METHOD FOR ANALYZING SPECIMEN - A pre-treatment method for analyzing a specified portion in a specimen composed of a biological tissue includes the steps of preparing a specimen to be analyzed, determining a specified portion in the specimen; and applying an analysis inhibitor to a portion except the specified portion of the specimen using a droplet spray method. | 2013-08-08 |
20130203106 | Apparatus and Method for Performing Experiments on Live Cells - An apparatus ( | 2013-08-08 |
20130203107 | METHOD FOR MEASURING THE AREA OF A SAMPLE DISPOSED WITHIN AN ANALYSIS CHAMBER - A method for determining the area of an analysis chamber covered by a biologic fluid sample quiescently residing within the chamber is provided. The chamber has a first panel with an interior surface, and a second panel with an interior surface, both of which panels are transparent. The method includes the steps of: a) illuminating the sample residing within the analysis chamber at one or more wavelengths operable to highlight interfaces between the sample and air, and to highlight a constituent within the sample; b) imaging the sample along the one or more wavelengths, and producing image signals representative of the interaction of the one or more wavelengths with the sample; c) determining a location of at least one interface between the sample and air, using the image signals; d) determining a location of one or more constituents within the sample relative to the at least one sample-air interface using the image signals; and e) determining an area of the chamber containing the sample, using the location of the one or more constituents and the at least one sample-air interface. | 2013-08-08 |
20130203109 | Methods and Compositions for Hematoxylin and Eosin Staining - The present invention provide for solutions of a defined composition useful in a staining protocol, such as a hematoxylin and eosin staining protocol, when used at certain points of the staining protocol. The formulations of these defined solutions are such that carry-over of the solutions will not negatively impact, or preferably, will stabilize or favorably modify staining reagent solutions coming in contact with the solutions. In certain embodiments of the invention, solutions are buffered to maintain a specific pH that when carried-over—such as carried-over into hematoxylin—will not significantly influence the pH of the next staining reagent. | 2013-08-08 |
20130203110 | SYSTEM FOR THE IN VITRO TRANSCRIPTION AND TRANSLATION OF MEMBRANE PROTEINS - System for the in vitro transcription and translation of membrane proteins comprising i) a micro-fluidic chip having at least one micro-fluidic reaction chamber and micro-fluidic channels to allow fluid to flow through the chip and into and from the at least one reaction chamber, ii) the at least one micro-fluidic reaction chamber being provided with at least one electrode base plate of conductive or semi-conductive material, and iii) lipid vesicles or a lipid membrane being bound or tethered to the at least one electrode base plate either directly or through spacer molecules. | 2013-08-08 |
20130203111 | Aldehyde Tags, Uses Thereof in Site-Specific Protein Modification - The invention features compositions and methods for site-specific modification of proteins by incorporation of an aldehyde tag. Enzymatic modification at a sulfatase motif of the aldehyde tag through action of a formylglycine generating enzyme (FGE) generates a formylglycine (FGly) residue. The aldehyde moiety of FGly residue can be exploited as a chemical handle for site-specific attachment of a moiety of interest to a polypeptide. | 2013-08-08 |
20130203112 | Site-Specific Incorporation of Phosphoserine Into Proteins in Escherichia Coli - Nucleic acids encoding mutant elongation factor proteins (EF-Sep), phosphoseryl-tRNA synthetase (SepRS), and phosphoseryl-tRNA (tRNA | 2013-08-08 |
20130203113 | METHOD OF STABILIZING mRNA - There is provided a method to increase the production of a desired protein in a microorganism by introduction of slowly translated codons in the encoding DNA gene sequence capable of slowing down the translation speed of the ribosomes moving along the mRNA, whereby the ribosomes protect the mRNA from being enzymatically degraded. This increases the stability of the mRNA transcript and thus results in an increased production of the desired protein. Moreover, there is provided a method of decreasing the half-life of a mRNA transcript from a gene encoding a peptide. | 2013-08-08 |
20130203114 | Regulation of Inducible Promoters - The present invention relates to the production of heterologous polypeptides in a recombinant bacterial host cell, wherein the bacterial host cell is rendered inable to deactivate the promoter controlling the expression of the heterologous polypeptide in the absence of an inducer. | 2013-08-08 |
20130203115 | MODIFIED NUCLEOSIDES, NUCLEOTIDES, AND NUCLEIC ACIDS, AND USES THEREOF - The present disclosure provides modified nucleosides, nucleotides, and nucleic acids, and methods of using thereof. | 2013-08-08 |
20130203116 | EXPRESSION AND PURIFICATION OF FUSION PROTEIN WITH MULTIPLE MBP TAGS - The present invention provides a new method for recombinantly expressing a protein of interest, such as the human BR-CA2 protein, BLM protein, CtIP protein, or EXOI protein, by expressing the protein in the form of a fusion protein comprising two maltose-binding protein (MBP) or glutathione-S-transferase (GST) tags. The expression cassette useful for this method and the fusion protein produced by this method are also described. | 2013-08-08 |
20130203117 | MANUFACTURING METHOD FOR SUGAR SOLUTION AND DEVICE FOR SAME - A method produces a sugar liquid by repeating a sugar liquid production process including (1) to (3): (1) adding a filamentous fungus-derived cellulase to cellulose to perform primary hydrolysis; (2) adding a fresh filamentous fungus-derived cellulase to the hydrolysate in Step (1) to perform secondary hydrolysis; and (3) subjecting the hydrolysate in Step (2) to solid-liquid separation to obtain a sugar liquid, from which a recovered enzyme is obtained; wherein the recovered enzyme obtained in Step (3) is used for Step (1) of the next and later sugar liquid production processes. | 2013-08-08 |
20130203118 | Whole-Cell Biocatalyst - The invention relates to a nucleic acid molecule comprising a section that encodes a signal peptide, a section that comprises a heterologous redox factor-regenerating polypeptide, an optional section that encodes a protease detection site, a section that encodes a transmembrane linker, and a section that encodes a transporter domain of an autotransporter or a variant thereof. The nucleic acid molecule enables the expression of redox factor-regenerating enzymes. | 2013-08-08 |
20130203119 | NUCLEIC ACID MODIFYING ENZYMES - This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid. | 2013-08-08 |
20130203120 | METHOD FOR CELL LYSIS AND PCR WITHIN THE SAME REACTION CHAMBER - A method for amplification of a target DNA, comprising the steps of (i) transferring a liquid with a first volume comprising at least one or more living cells into a vessel (ii) adding to said vessel a PCR reaction buffer with a second volume, whereas said second volume is at least 2× as large as said first volume (iii) lysing said at least one or more living cells within said vessel by means of incubation for at least 1 Minute at at least 90° C., and (iv) amplifying said target by means of a polymerase chain reaction without performance of an intermediate purification step. | 2013-08-08 |
20130203121 | METHODS FOR THE SEMI-SYNTHETIC PRODUCTION OF HIGH PURITY "MINICIRCLE" DNA VECTORS FROM PLASMIDS - The invention relates to methods and reagents for producing DNA vectors, in particular minicircle (MC) DNA vectors, in superhelical form. The invention further relates to highly pure preparations of circular DNA vectors, in particular MC DNA vectors. | 2013-08-08 |
20130203122 | Reduced Inhibition of One-Step RT-PCR - The present invention provides a method for amplifying a nucleic acid molecule. The method involves mixing an RNA template with a composition having a reverse transcriptase, a DNA polymerase and a RT inhibition reducer. The RT inhibition reducer can be Sso7d, Sac7d, Sac7e, Sso7e, AluI methylase, suramin, a phosphorothioate oligodeoxycytosine, a phosphorothioate oligodeoxyadenine, a phosphorothioate oligodeoxythymine or poly(rA)(dT). The mixing forms a mixture that is incubated under conditions sufficient to synthesize a DNA molecule complementary to all or a portion of the RNA template, thereby amplifying the nucleic acid molecule. | 2013-08-08 |
20130203123 | CLOSED NUCLEIC ACID STRUCTURES - The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications. | 2013-08-08 |
20130203124 | Alpha-Amylase Variant With Altered Properties - The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase. | 2013-08-08 |
20130203125 | CELLULOSIC PROCESSING TRAIT DEVELOPMENT USING A THERMOREGULATED, INTEIN-MODIFIED XYLANASE - In planta consolidated bioprocessing has the advantages of decreasing biomass pretreatment costs, utilizing excess plant protein production capacity for enzyme production, and decreasing mass transfer resistance of enzyme diffusion to its substrate. However, in planta expression of cell wall degrading (CWD) enzymes often leads to detrimental plant phenotypes that impact crop yield. To provide in planta CWD enzyme activity without any adverse phenotype, a thermostable xylanase, XynB (EC 3.2.1.8), was engineered with a thermoregulated intein, Tth-HB27 DnaE-1 (Tth intein), that controls its hydrolytic activity through conditional intein splicing. Maize plants expressing the heat inducible Tth intein-modified XynB developed normally, yet possessed enhanced post harvest glucose production from dried corn stover. Expression of CWD enzymes as dormant, intein-modified proteins that can be activated by heat treatment after harvest provides the basis for developing a novel cellulosic processing trait in plants. | 2013-08-08 |
20130203126 | Alpha-Amylases - The present invention relates to alpha-amylases, nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, and methods of using the alpha-amylases. | 2013-08-08 |