03rd week of 2013 patent applcation highlights part 33 |
Patent application number | Title | Published |
20130017512 | DENTAL IMPLANT SYSTEM FOR PROMOTING OSSEOINTEGRATIONAANM Tzeng; Yu-HongAACI Taipei CityAACO TWAAGP Tzeng; Yu-Hong Taipei City TW - A dental implant system for promoting osseointegration comprises an implant in cylindrical shape having a thread division at bottom, which can be screwed in the oral alveolar bone of a patient at the location of the implant: where the implant has a neck part on top being located at the same level as the cortical bone of the alveolar bone, as the implant is screwed in the alveolar bone; characterized in that: the surface of the neck part has uneven lines, of which the gapes keep the bone blood on the neck part surface and therefore promote cortical bone growing. | 2013-01-17 |
20130017513 | DENTAL IMPLANTAANM Lutz; WalterAACI WertheimAACO DEAAGP Lutz; Walter Wertheim DEAANM Ries; StefanAACI WertheimAACO DEAAGP Ries; Stefan Wertheim DE - A dental implant comprises an implant screw ( | 2013-01-17 |
20130017514 | Method of Manufacturing Ceramic Primary Crown and Ceramic Primary Crown Manufactured by the SameAANM Kim; Yong-SuAACI BusanAACO KRAAGP Kim; Yong-Su Busan KRAANM Jeon; Hyun-JunAACI BusanAACO KRAAGP Jeon; Hyun-Jun Busan KRAANM Oh; Kyung SikAACI IncheonAACO KRAAGP Oh; Kyung Sik Incheon KRAANM Kim; Sung KiAACI BusanAACO KRAAGP Kim; Sung Ki Busan KRAANM Kim; Se-HoonAACI Gyeonggi-doAACO KRAAGP Kim; Se-Hoon Gyeonggi-do KRAANM Kim; Joon HyungAACI Gyeonggi-doAACO KRAAGP Kim; Joon Hyung Gyeonggi-do KRAANM Hong; Young PyoAACI Gangwon-doAACO KRAAGP Hong; Young Pyo Gangwon-do KR - A method of manufacturing a ceramic primary crown including mixing a source material including zirconia or alumina that configures a frame of a primary crown, a polymer for reducing viscosity and applying ductility upon injection molding, and a toner for providing the same or similar color as baby teeth, heating the polymer contained in the mixed source material to have ductility, injection molding the heated source material, extracting the polymer to reduce brittleness and increase ductility with respect to the injection-molded matter, removing fat to completely eliminate the polymer element from the injection-molded matter, from which the polymer is partially extracted through the polymer extraction, sintering the fat-removed body, from which the polymer is removed, to improve mechanical properties, and polishing an outer surface of the sintered body to provide gloss, and performing barrel-finishing to remove a burr. | 2013-01-17 |
20130017515 | LASER TRAINER TARGET - A target senses whether and where it has been struck by a laser light. The target includes a screen for allowing laser light to pass through, a plurality of light sensors behind the screen, and an optical display associated with one or more of the light sensors. When the target is struck by a laser light, a sensor records the strike, and the target may be struck with laser light multiple times, whereby different sensors may record the different strikes. To determine whether and where the target has been struck, the user activates a display mode that causes the optical displays in the target to illuminate. The user can then reset the target so the user can begin again. | 2013-01-17 |
20130017516 | ACTIVE BRAILLE TIMEPIECE & RELATED METHODSAANM Tyler; KellyAACI JamulAAST CAAACO USAAGP Tyler; Kelly Jamul CA US - A timepiece adapted for efficient mechanical display of time and date according to a condensed Braille number format, the timepiece including a Braille actuation mechanism comprising a plurality of servo motors, translational pins, and similar components. The timepiece is adapted to display time (hour:minute), and date (month:day) using Braille character cells in accordance with a condensed Braille format. Additionally, the timepiece is adapted for scrollable Braille representations such that a user can set the time, date, and an optional alarm. Certain methods for providing a Braille display of numeric information are further disclosed. | 2013-01-17 |
20130017517 | METHOD AND APPARATUS FOR LEAVING MESSAGES FOR PASSED LOVED ONESAANM SCIANNA; CARL ROYAACI LisleAAST ILAACO USAAGP SCIANNA; CARL ROY Lisle IL US - An apparatus and method for people to leave a message to a passed loved one includes a web site established to provide potential users with a description of the service available. The website may explain the website's owner, a therapist's credentials, and may explain the concept of providing closure with the methods and apparatus of the present invention. The website may provide information regarding a cost to the user. The website will direct the user to call a certain phone number to leave a message for their passed loved one. | 2013-01-17 |
20130017518 | Method of Permanently Removing Repeating Upsetting Emotions and Behaviors Associated with a Person, Persons, Events, and Situations - The present invention is a method to permanently remove repeating upsetting emotions and behaviors that are associated with a person, persons, events, and situations. This method is intended for use as an application with electronic devices in which instructions can be displayed to a user through a graphic user interface. The user performs an emotional transformation process by following the instructions which can be in the form of steps, processes, or imagination exercises. The emotional transformation process eliminates the repeating patterns that cause repeating upsetting emotions and replaces the upsetting emotions with positive emotions. The emotional transformation process takes place within the user's imagination in which feelings, thoughts, and emotions are formed into images within the imagination. The user is not required to input specific details about a troubling issue causing the upsetting emotions, nor is the user required to analyze the specific details and troubling issues. | 2013-01-17 |
20130017519 | SYSTEM AND METHODS FOR MONITORING AND ADJUSTING HUMAN BEHAVIORAL PATTERNS AND CONDITIONS - A system and methods for delivering “behavioral modification content” to a user. Behavioral modification content includes any information that can be perceived by the user, including perceived visually, aurally, tangibly, or aromatically by the user for the purpose of reminding the user to temporarily remove himself or herself from the context in the person is operating and attempt to better understand that context. Embodiments of the present invention also permit the user to create and save reflections about the behavioral modification content. | 2013-01-17 |
20130017520 | Multi-Sensory ManipulationAANM Yoo; HerbAACI BeavertonAAST ORAACO USAAGP Yoo; Herb Beaverton OR USAANM Reichow; Alan W.AACI BeavertonAAST MOAACO USAAGP Reichow; Alan W. Beaverton MO US - Aspects of the present invention relate to a multi-sensory manipulation system. The multi-sensory manipulation system is useable to train one or more senses through the manipulation of one or more sensory inputs as perceived by a user. The multi-sensory system may be used train a variety of senses, such as vision, hearing, olfactory, taste, touch, and the like. Consequently, the multi-sensory system may be comprised of a first sensory vitiation device that vitiates a sensory input for the first sense. The multi-sensory system may be comprised of a first sensory vitiation driver that generates vitiations instructions useable by the first sensory vitiation device. The multi-sensory system may also be comprised of a controller to coordinate one or more sensory drivers and/or one or more sensory vitiation devices to allow for the training of one or more senses through the manipulation of multiple sensory inputs. | 2013-01-17 |
20130017521 | Swim trainer vestAANM Sykes; MarvinAACI CliftonAAST NJAACO USAAGP Sykes; Marvin Clifton NJ US - A swim trainer vest comprising a swimmer support handle to hold the child up horizontally in water and instruct the child to perform swim strokes so that a child can become a better swimmer. The swim trainer vest has adjustable safety straps and safety latches to fit the swim trainer vest on children with different body circumferences and weights. | 2013-01-17 |
20130017522 | METHOD AND APPARATUS FOR DELIVERING A LEARNING SESSIONAANM Nguyen; ChrisAACI BaltimoreAAST MDAACO USAAGP Nguyen; Chris Baltimore MD USAANM Hoehn-Saric; ChrisAACI MiamiAAST FLAACO USAAGP Hoehn-Saric; Chris Miami FL USAANM Clinton; LeeAACI BaltimoreAAST MDAACO USAAGP Clinton; Lee Baltimore MD US - A learning system includes at least one student workstation in a first learning environment which holds a learning session for a student. The system generates and displays primary instructional material to the student in accordance with student profile data for the student. The system causes supplemental material or content to be generated and displayed to the student at the student workstation when a downtime interval is triggered. The system generates the instructional material in accordance with the student profile data for the student. The system allows the student to seamlessly continue the learning session in a second, different learning environment. The system further allows the student to seamlessly complete the learning session in a third, different learning environment. The disclosed system thus enables students to work in various different learning environments and reduce the impact of interruptions in the learning experience. | 2013-01-17 |
20130017523 | UTILIZING FAILURES IN QUESTION AND ANSWER SYSTEM RESPONSES TO ENHANCE THE ACCURACY OF QUESTION AND ANSWER SYSTEMS - A method of enhancing the accuracy of a question-answer system. Missing information from a corpus of data is identified. The missing information is any information that improves a confidence for a candidate answer to a question. A follow-on inquiry is generated. The follow-on inquiry prompts for the missing information to be provided. The follow-on inquiry is output to an external source. A response to the follow-on inquiry is received from the external source. The response is added to the corpus of data. | 2013-01-17 |
20130017524 | UTILIZING FAILURES IN QUESTION AND ANSWER SYSTEM RESPONSES TO ENHANCE THE ACCURACY OF QUESTION AND ANSWER SYSTEMS - A computerized device for enhancing the accuracy of a question-answer system. The computerized device comprises a question-answer system comprising software for performing a plurality of question answering processes. A receiver receives a question into the question-answer system. A processor that generates a plurality of candidate answers to the question is connected to the question-answer system. The processor determines a confidence score for each of the plurality of candidate answers. The processor evaluates sources of evidence used to generate the plurality of candidate answers. The processor identifies missing information from a corpus of data. The missing information comprises any information that improves a confidence score for a candidate answer. The processor generates at least one follow-on inquiry based on the missing information. A network interface outputs the at least one follow-on inquiry to external sources separate from the question-answer system. | 2013-01-17 |
20130017525 | METHOD AND APPARATUS FOR SCORING A LEARNING SESSIONAANM Nguyen; ChrisAACI BaltimoreAAST MDAACO USAAGP Nguyen; Chris Baltimore MD USAANM Hoehn-Saric; ChrisAACI MiamiAAST FLAACO USAAGP Hoehn-Saric; Chris Miami FL USAANM Clinton; LeeAACI BaltimoreAAST MDAACO USAAGP Clinton; Lee Baltimore MD US - Methods and apparatus for displaying data on a multi-user display device are disclosed. A first subset of the data is displayed in a first orientation, such that the displayed data is in a proper orientation with respect to a first user located at a first side of the display device. Upon receiving a user input requesting to share the data, a second subset of the data is displayed in a second different orientation, such that the displayed data is in the proper orientation with respect to a second user located at a second different side of the display device. Which subset of the data is displayed to which user is based at least in part on identifying information associated with each user. | 2013-01-17 |
20130017526 | METHOD AND APPARATUS FOR SHARING A TABLET COMPUTER DURING A LEARNING SESSIONAANM Nguyen; ChrisAACI BaltimoreAAST MDAACO USAAGP Nguyen; Chris Baltimore MD USAANM Hoehn-Saric; ChrisAACI MiamiAAST FLAACO USAAGP Hoehn-Saric; Chris Miami FL USAANM Clinton; LeeAACI BaltimoreAAST MDAACO USAAGP Clinton; Lee Baltimore MD US - Methods and apparatus for displaying data on a multi-user display device are disclosed. A first subset of the data is displayed in a first orientation, such that the displayed data is in a proper orientation with respect to a first user located at a first side of the display device. Upon receiving a user input requesting to share the data, a second subset of the data is displayed in a second different orientation, such that the displayed data is in the proper orientation with respect to a second user located at a second different side of the display device. Which subset of the data is displayed to which user is based at least in part on identifying information associated with each user. | 2013-01-17 |
20130017527 | METHOD AND APPARATUS FOR REWARDING A STUDENTAANM Nguyen; ChrisAACI BaltimoreAAST MDAACO USAAGP Nguyen; Chris Baltimore MD USAANM Hoehn-Saric; ChrisAACI MiamiAAST FLAACO USAAGP Hoehn-Saric; Chris Miami FL USAANM Wenck; EricAACI BaltimoreAAST MDAACO USAAGP Wenck; Eric Baltimore MD USAANM Craig; JeffAACI AnnapolisAAST MDAACO USAAGP Craig; Jeff Annapolis MD USAANM Clinton; LeeAACI BaltimoreAAST MDAACO USAAGP Clinton; Lee Baltimore MD US - Methods and apparatus for rewarding students with redeemable tokens during a lesson are disclosed. As the student works through the lesson, the system determines whether any designated performance events occur based on the student's performance. Based on any occurrences of performance events, the system may accumulate one or more tokens for the student. The system may provide a visual indication of any tracked occurences of performance events. For example, for each occurrence of a performance event, the system increments a progress meter by a designated amount. When progress meter reaches a designated level, the system accumulates a designated quantity of tokens for the student. The amount by which the progress meter increments for each occurrence of a performance event may be based on the type of performance event which occurs. Different types of performance events may have different values or contribute differently toward the accumulation of tokens. | 2013-01-17 |
20130017528 | METHOD AND APPARATUS FOR MANAGING STUDENT ACTIVITIESAANM Nguyen; ChrisAACI BaltimoreAAST MDAACO USAAGP Nguyen; Chris Baltimore MD USAANM Hoehn-Saric; ChrisAACI MiamiAAST FLAACO USAAGP Hoehn-Saric; Chris Miami FL USAANM Wenck; EricAACI BaltimoreAAST MDAACO USAAGP Wenck; Eric Baltimore MD USAANM Craig; JeffAACO USAAGP Craig; Jeff USAANM Clinton; LeeAACI BaltimoreAAST MDAACO USAAGP Clinton; Lee Baltimore MD US - A learning system includes at least one student workstation which holds a learning session for a student. The system generates and displays primary instructional material to the student in accordance with student profile data for the student. The system causes supplemental material or content to be generated and displayed to the student at the student workstation when a downtime interval is triggered. The system generates the supplemental material in accordance with the student profile data for the student. The disclosed system thus enables downtime to be more appropriately utilized by the student, in a manner that accommodates the learning preferences and skill gaps of the student and/or that rewards the student for showing proficiency at a skill. | 2013-01-17 |
20130017529 | METHOD AND APPARATUS FOR GENERATING EDUCATIONAL CONTENTAANM Nguyen; ChrisAACI BaltimoreAAST MDAACO USAAGP Nguyen; Chris Baltimore MD USAANM Hoehn-Saric; ChrisAACI MiamiAAST FLAACO USAAGP Hoehn-Saric; Chris Miami FL USAANM Clinton; LeeAACI BaltimoreAAST MDAACO USAAGP Clinton; Lee Baltimore MD US - A learning system includes at least one student workstation which holds a learning session for a student. The system generates and displays primary instructional material to the student in accordance with student profile data for the student. The system causes supplemental material or content to be generated and displayed to the student at the student workstation when a downtime interval is triggered. The system generates the supplemental material in accordance with the student profile data for the student. The disclosed system thus enables downtime to be more appropriately utilized by the student, in a manner that accommodates the learning preferences and skill gaps of the student and/or that rewards the student for showing proficiency at a skill. | 2013-01-17 |
20130017530 | METHOD AND APPARATUS FOR TESTING STUDENTSAANM Nguyen; ChrisAACI BaltimoreAAST MDAACO USAAGP Nguyen; Chris Baltimore MD USAANM Hoehn-Saric; ChrisAACI MiamiAAST FLAACO USAAGP Hoehn-Saric; Chris Miami FL USAANM Wenck; EricAACI BaltimoreAAST MDAACO USAAGP Wenck; Eric Baltimore MD USAANM Craig; JeffAACI AnnapolisAAST MDAACO USAAGP Craig; Jeff Annapolis MD USAANM Nyugen; ChrisAACI BaltimoreAAST MDAACO USAAGP Nyugen; Chris Baltimore MD USAANM Fontana; LynnAACI BaltimoreAAST MDAACO USAAGP Fontana; Lynn Baltimore MD USAANM Clinton; LeeAACI BaltimoreAAST MDAACO USAAGP Clinton; Lee Baltimore MD US - The learning system disclosed herein enables a performance assessment to be incorporated or integrated into instructional materials being administered to a student as part of the student's current learning prescription. As the student progresses through the instructional materials, the system collects and maintains data regarding the student's performance. When data indicate that the student is not meeting certain educational goals, the system adjusts or alters the student's learning prescription to accommodate the student's current needs. | 2013-01-17 |
20130017531 | TESTING SYSTEM AND METHOD USING AN IPSATIVE SCALEAANM Sung; Yao-TingAACI Taipei CityAACO TWAAGP Sung; Yao-Ting Taipei City TW - A testing system using an ipsative scale is applicable to a data processing device. The testing system includes a test question module for generating test questions comprising questions, a measuring scale and a plurality of labels, and a responding module for moving each of the labels to a corresponding position on the measuring scale in response and according to the question, and a recording module for recording information about the position of each label as a fall point, wherein the fall point information compares one or more test-takers for obtaining a correlation between the test questions of each label, thereby analyzing test results of one or more test-takers and reducing occurrence of faked test results. | 2013-01-17 |
20130017532 | Method and device for preserving the vitality and function of a harvested blood vessel - Blood vessel preservation technology is disclosed wherein harvested blood vessels are attached to stationary attachment fixtures at one end, and to a non-stationary (freely extending) fixture at the other end. The vessel and fixtures are placed within a container. Thereafter, a stream of pulsatile blood is directed through the fixtures and the lumen of the vessel causing the vessel to assume full natural length and aligning the vessel with the container. The pulsatile blood then completely bathes the outside surface of the vessel before being re-circulated. The methods disclosed include those wherein the patient's own circulatory system is part of the circulatory path flowing through the vessel and methods in which the circulatory path is outside and apart from the patient. A pulsatile pump, blood heater and pressure regulator may all be utilized in preferred embodiments of the disclosed methods and devices. | 2013-01-17 |
20130017533 | Perfusion Culture Method and Perfusion Culture Device for Organ or TissueAANM Tsuji; TakashiAACI Shinjuku-kuAACO JPAAGP Tsuji; Takashi Shinjuku-ku JPAANM Nakao; KazuhisaAACI Shinjuku-kuAACO JPAAGP Nakao; Kazuhisa Shinjuku-ku JP - Disclosed are a perfusion culture method and a perfusion culture device whereby a perfusate can be delivered to all parts of an organ or tissue so that the organ or tissue can be preserved while sufficiently maintaining the function thereof. Specifically disclosed is a method for perfusion culture of an organ or tissue removed from a mammal, said organ or tissue having been removed together with a second organ or tissue connected to the aforesaid organ or tissue in vivo, which comprises a step for fixing said second organ or tissue so as to hang the aforesaid organ or tissue and a step for perfusing blood vessels in the aforesaid organ or tissue with a perfusate. | 2013-01-17 |
20130017534 | DEVICE AND METHOD FOR IDENTIFYING MICROBES AND COUNTING MICROBES AND DETERMINING ANTIMICROBIAL SENSITIVITY - A method of determining antimicrobial activity of an agent can include providing a well, wherein the well contains at least one antimicrobial agent, the well further including at least two electrodes. A sample of a microbe can be added into the well and a voltage pulsed between the electrodes. An electrical property can be sampled and recorded. In another aspect, a method of identifying at least one microbe includes taking a sample containing the at least one microbe, isolating the at least one microbe from the sample, dividing the at least one microbe into a at least one well, wherein each well contains at least one antimicrobial agent and at least two electrodes. A voltage is pulsed between the at least two electrodes, an electrical property is sampled during the pulsing and recorded. In another aspect, a diagnostic device for detecting at least one microbe is presented. | 2013-01-17 |
20130017535 | INSTRUMENT AND PROCESS FOR THE AUTOMATED PROCESSING OF LIQUID SAMPLESAANM Frey; RolfAACI EbikonAACO CHAAGP Frey; Rolf Ebikon CHAANM Schacher; GottliebAACI KriensAACO CHAAGP Schacher; Gottlieb Kriens CH - An automated instrument and process for processing samples is presented. The instrument comprises a sampling area for receiving samples and reaction vessels; an analytical area with a first device resource comprising at least one analyzer; a reaction area comprising a conveyor for reagent containers; an incubator; a second device resource comprising first functional devices, the first functional devices having access to the sampling area and the incubator such as to transfer reaction vessels from the sampling area to the incubator and/or to pipette samples and/or reagents into the reaction vessels; a third device resource comprising second functional devices, the second functional devices having access to the incubator and the analytical area such as to transfer the reaction vessels from the incubator to the analytical area and/or to dispense liquids or withdraw liquids from the reaction vessels. | 2013-01-17 |
20130017536 | WESTERN BLOT KIT FOR DETECTION OF VACCINATED POULTRYAANM Madani; RasoolAACI KarajAACO IRAAGP Madani; Rasool Karaj IRAANM Rezayat; Seyed MahdiAACI TehranAACO IRAAGP Rezayat; Seyed Mahdi Tehran IRAANM Sarkar; SaeedAACI TehranAACO IRAAGP Sarkar; Saeed Tehran IRAANM Emami; TaraAACI KarajAACO IRAAGP Emami; Tara Karaj IR - Modified western blot membranes with silver nanoparticle allow the small peptides of the NS1 protein of the poultry influenza virus to be kept in the membrane and not to diffuse during transferring from the Tricine SDS PAGE. These peptides may differentiate infected from vaccinated poultry. | 2013-01-17 |
20130017537 | METHOD OF IN-VITRO IMMUNOASSAY - The present invention relates to a novel method of in-vitro assay of the molecules of the extracellular matrix synthesized by cells in culture and uses thereof in the form of a kit for in-vitro measurement and/or for a method of screening of cosmetic and/or pharmaceutical ingredients. | 2013-01-17 |
20130017538 | DEVICES, SYSTEMS, AND METHODS FOR MAGNETIC SEPARATIONAANM Ionescu-Zanetti; CristianAACI BerkeleyAAST CAAACO USAAGP Ionescu-Zanetti; Cristian Berkeley CA USAANM Nevill; Joshua TannerAACI El CerritoAAST CAAACO USAAGP Nevill; Joshua Tanner El Cerrito CA USAANM Schwartz; MichaelAACI OaklandAAST CAAACO USAAGP Schwartz; Michael Oakland CA USAANM Conant; Carolyn G.AACI San FranciscoAAST CAAACO USAAGP Conant; Carolyn G. San Francisco CA USAANM Rudoff; RogerAACI CupertinoAAST CAAACO USAAGP Rudoff; Roger Cupertino CA US - Methods, microfluidic devices, and instruments for magnetic separation of particles from a fluid are described. Examples include microfluidic devices having a removable portion. Examples include microfluidic devices having one or more regions of reduced fluid velocity. Examples further including instruments having pneumatic interfaces. Examples further includes instruments having controllable magnets, imaging components, or combinations thereof. | 2013-01-17 |
20130017539 | METHODS AND COMPOSITIONS FOR CHLAMYDIA TRACHOMATIS DIAGNOSTIC TESTING - The invention provides methods, reagent, and kits for detecting the presence of | 2013-01-17 |
20130017540 | IDENTIFICATION OF MUTATION TYPES ASSOCIATED WITH ACQUIRED RESISTANCE AND METHODS FOR USING SAME - Methods for identifying or classifying a gene mutation type associated with acquired drug resistance of cancer is provided. Said methods may include determining a total copy number (N) of a susceptible gene in a cancer cell, identifying a mutant copy number of the susceptible gene, determining a mutant copy number sufficient to cause acquired drug resistance (M); and comparing N with M to identify or classify the mutation type in the cancer cell. | 2013-01-17 |
20130017541 | Methods and Compositions for Segregating Target Nucleic Acid from Mixed Nucleic Acid Samples - The invention provides methods, compositions and kits for segregating a target nucleic acid from a mixed nucleic acid sample. The methods, compositions and kits comprise a non-processive endonuclease (e.g., a restriction enzyme) or an antibody that binds the target nucleic acid (e.g., has methylation specificity). The mixed nucleic acid sample can comprise prokaryotic and eukaryotic nucleic acid and/or nucleic acid from more than one prokaryotic or eukaryotic organisms. | 2013-01-17 |
20130017542 | Method and Kit for Amplifying and Detecting PolynucleotideAANM Hosomi; ToshiyaAACI Kyoto-shiAACO JPAAGP Hosomi; Toshiya Kyoto-shi JPAANM Hirai; MitsuharuAACI Kyoto-shiAACO JPAAGP Hirai; Mitsuharu Kyoto-shi JP - Disclosed is a method of amplifying a polynucleotide, comprising:
| 2013-01-17 |
20130017543 | Method and Kit for Amplifying and Detecting PolynucleotideAANM Hosomi; ToshiyaAACI Kyoto-shiAACO JPAAGP Hosomi; Toshiya Kyoto-shi JPAANM Hirai; MitsuharuAACI Kyoto-shiAACO JPAAGP Hirai; Mitsuharu Kyoto-shi JP - Disclosed is a method of amplifying a polynucleotide, comprising:
| 2013-01-17 |
20130017544 | High Resolution Melting Analysis on a Droplet Actuator - An integrated droplet actuator device and methods are provided for performing PCR amplification and high-resolution melting (HRM) analysis on a single droplet actuator. HRM analysis can be used in combination with PCR amplification for detection of sequence variations (e.g., single-nucleotide polymorphisms, nucleotide-repeat polymorphisms, mutation scanning and assessment of DNA methylation) within one or more genes of interest. The PCR amplicons can be fluorescently labeled during amplification using a saturating DNA intercalating fluorescent dye, a 5′-labeled primer, or labeled probes. Also provided are a droplet actuator device and methods for sample preparation using the droplet actuator and detection of sequence variations on the same droplet actuator. | 2013-01-17 |
20130017545 | APPARATUS AND METHODS FOR ACQUIRING ANALYTES FROM A DRIED BIOLOGICAL FLUID SAMPLEAANM Yong; BenAACI Glenn DaleAAST MDAACO USAAGP Yong; Ben Glenn Dale MD USAANM Hudson; William C.AACI TustinAAST CAAACO USAAGP Hudson; William C. Tustin CA US - An apparatus for acquiring analytes from a dried biological fluid sample includes a tube, a substrate in a proximal section of the tube, and a sorbent bed in a distal section of the tube. A biological fluid sample is dispensed on the substrate and is dried to form a dried sample. A conditioning solvent is flowed into the distal section to condition the sorbent bed. A first elution solvent is flowed through the substrate and the sorbent bed. Analytes are eluted from the dried sample and retained on the sorbent bed. A second elution solvent is flowed through the substrate and the sorbent bed. The analytes are eluted from the sorbent bed, pass through an opening, and are collected. Alternatively, an elution solvent is flowed through the substrate and the sorbent bed such that analytes eluted from the dried sample pass through the sorbent bed and the opening for collection, while non-analytes are retained on the sorbent bed. | 2013-01-17 |
20130017546 | Breast Tumor Markers and Methods of Use ThereofAANM Grifantini; RenataAACI SienaAACO ITAAGP Grifantini; Renata Siena ITAANM Pileri; PieroAACI SienaAACO ITAAGP Pileri; Piero Siena ITAANM Campagnoli; SusannaAACI SienaAACO ITAAGP Campagnoli; Susanna Siena ITAANM Grandi; AlbertoAACI SienaAACO ITAAGP Grandi; Alberto Siena ITAANM Parri; MatteoAACI SienaAACO ITAAGP Parri; Matteo Siena ITAANM Pierleoni; AndreaAACI SienaAACO ITAAGP Pierleoni; Andrea Siena ITAANM Nogarotto; RenzoAACI SienaAACO ITAAGP Nogarotto; Renzo Siena IT - Newly identified proteins as markers for the detection of breast tumors, or as therapeutic targets for treatment thereof; affinity ligands capable of selectively interacting with the newly identified markers, as well as methods for tumor diagnosis and therapy using such ligands. | 2013-01-17 |
20130017547 | ORGAN-SPECIFIC FELINE ENDOTHELIAL CELLS AND USES THEREOFAANM Kieda; ClaudineAACI OrleansAACO FRAAGP Kieda; Claudine Orleans FRAANM Paprocka; MariaAACI WroclawAACO PLAAGP Paprocka; Maria Wroclaw PLAANM Mitterand; MicheleAACI ArdonAACO FRAAGP Mitterand; Michele Ardon FRAANM Lamerant-Fayel; NathalieAACI OlivetAACO FRAAGP Lamerant-Fayel; Nathalie Olivet FRAANM Boulouis; Henri-JeanAACI La Varenne Saint HilaireAACO FRAAGP Boulouis; Henri-Jean La Varenne Saint Hilaire FRAANM Haddad; NadiaAACI Saint-MauriceAACO FRAAGP Haddad; Nadia Saint-Maurice FRAANM Monteil; MartineAACI Villeneuve-Saint-GeorgesAACO FRAAGP Monteil; Martine Villeneuve-Saint-Georges FR - The present invention relates to isolated organ-specific feline endothelial cells. Specifically, the present invention relates to established organ-specific feline endothelial cells. The present invention also relates to organ-specific feline endothelial cells derived from micro- and macro-vascularisation. The present invention also relates to methods for screening molecules, for studying pathologies and for producing pathogens using same. | 2013-01-17 |
20130017548 | SCREENING METHOD - The present invention relates to a novel method for screening for a therapeutic or prophylactic agent for an inflammatory disease. | 2013-01-17 |
20130017549 | METHOD OF AMPLIFYING TARGET NUCLEIC ACID WITH REDUCED AMPLIFICATION BIAS AND METHOD FOR DETERMINING RELATIVE AMOUNT OF TARGET NUCLEIC ACID IN SAMPLEAANM HONG; Sung-wooAACI Gwangmyeong-siAACO KRAAGP HONG; Sung-woo Gwangmyeong-si KR - A method of amplifying a target nucleic acid with reduced amplification bias and a method of determining a relative amount of a target nucleic acid in a sample. | 2013-01-17 |
20130017550 | MAP KINASE KINASE KINASE KINASE 3 (MAP4K3) AS A BIOMARKER AND THERAPETIC TARGET FOR AUTOIMMUNE DISEASE, CANCER, INFLAMMATION AND IL-17-ASSOCIATED DISEASE - Methods for identifying a therapeutic agent for treating a Germinal Center Kinase (GCK)-Like Kinase (GLK)-mediated disease are disclosed. Methods for detecting a modulation of GLK signaling by a lest compound are disclosed. Also disclosed are methods for detecting the presence and/or severity of an autoimmune disease and/or cancer. | 2013-01-17 |
20130017551 | COMPUTATION OF REAL-WORLD ERROR USING META-ANALYSIS OF REPLICATES - A system, including methods and apparatus, for performing a digital assay on a number of sample-containing replicates, each containing a plurality of sample-containing droplets, and measuring the concentration of target in the sample. Statistical meta-analysis techniques may be applied to reduce the effective variance of the measured target concentration. | 2013-01-17 |
20130017552 | METHOD AND KIT FOR SEPARATING VIRAL AND PROKARYOTIC NUCLEIC ACIDS FROM EUKARYOTIC NUCLEIC ACIDSAANM Rudorfer; WalterAACI St. Oswald/FreistadtAACO ATAAGP Rudorfer; Walter St. Oswald/Freistadt AT - The invention relates to a method for at least partially separating viral and/or prokaryotic nucleic acids from eukaryotic nucleic acids from a biological sample, in particular a eukaryotic cell suspension, comprising the following steps in the following order: a) re-suspending the cells in the presence of a chelating agent, in particular EDTA or EDTA in combination with a saccharide, b) lysis of the cells by chemical lysis, such as alkaline lysis, enzymatic lysis and/or boiling lysis and/or mechanical lysis, c) neutralizing the cell lysate and d) separating the precipitated eukaryotic nucleic acids and obtaining the viral and/or prokaryotic nucleic acids, as well as the use of a kit comprising a re-suspension buffer with chelating agent and optionally a saccharide and RNAse, lysis buffer comprising at least one base and a detergent and neutralizing buffer for at least partially separating viral and/or prokaryotic nucleic acids from eukaryotic nucleic acids from a biological sample, in particular a eukaryotic cell suspension. | 2013-01-17 |
20130017553 | PIPETTES, METHODS OF USE, AND METHODS OF STIMULATING AN OBJECT OF INTEREST - The present invention features a freestanding microfluidic pipette with both solution exchange capability and fluid re-circulation, enabling highly localized and contamination- free fluid delivery within a confined volume in the vicinity of the pipette exit. Preferably, the device features direct positioning, so the pipette can be directed at a point or object of interest, such as a biological or artificial cell, a defined surface area or a sensor element within an open volume, using micro- or nanopositioning techniques. One aspect of the invention provides a free-standing pipette. The free-standing pipette includes a microfluidic device comprising one or more channels with exits leading into an open-volume and a positioning device programmed to hold the microfluidic device at an angle from a horizontal axis. | 2013-01-17 |
20130017554 | SUMO-SPECIFIC AFFINITY TAG - A new SUMO-specific affinity tag is described herein, based on the amino acid sequence from 403-621 of the SUMO protease Ulp1, and containing a crucial substitution of serine for cysteine. This affinity tag is particularly useful for a range of applications, including detection and affinity purification of sumoylated proteins from cell extracts. | 2013-01-17 |
20130017555 | FACS-BASED METHOD FOR OBTAINING AN ANTIBODY SEQUENCE - In certain embodiments, the method may comprises: a) contacting a population of permeabilized, cross-linked antibody-producing cells with a labeled antigen to produce a labeled sample in which cells that produce an antibody that specifically binds to said antigen are intracellularly labeled; b) using FACS to isolate cells that are intracellularly labeled, thereby producing labeled cells; c) uncrosslinking said labeled cells to produce uncrosslinked cells; and d) amplifying heavy and light chain-encoding nucleic acid from individual uncrosslinked cells, thereby obtaining nucleic acid that encodes the variable domain of antibody that specifically binds to said antigen. | 2013-01-17 |
20130017556 | ASSAYS FOR HDL BIOMOLECULAR INTERACTIONS - The invention relates to methods, compositions and kits for detecting molecular interactions. In one embodiment, the invention relates to methods for quantifying HDL interactions with biomolecules. In still another embodiment, the invention relates to methods for determining HDL function. | 2013-01-17 |
20130017557 | COMBINATION OF sPLA2 TYPE IIA MASS AND OXPL/APOB CARDIOVASCULAR RISK FACTORS FOR THE DIAGNOSIS/PROGNOSIS OF A CARDIOVASCULAR DISEASE/EVENTAANM Mallat; ZiadAACI ParisAACO FRAAGP Mallat; Ziad Paris FRAANM Tedgui; AlainAACI ParisAACO FRAAGP Tedgui; Alain Paris FRAANM Tsimikas; SotiriosAACI La JollaAAST CAAACO USAAGP Tsimikas; Sotirios La Jolla CA USAANM Witztum; JosephAACI La JollaAAST CAAACO USAAGP Witztum; Joseph La Jolla CA US - The present invention related to a method of identifying a subject having or at risk of having or developing a cardiovascular disease and/or a cardiovascular event, comprising: —measuring, in a sample obtained from said subject, at least two cardiovascular risk factors: a) sPLA2 type HA mass and b) oxidized phospholipids on apolipoprotein B-IOO particles (OxPL/apoB), —combining said measurements, the combined value of sPLA2 type HA mass and OxPL/apoB being indicative of having or a risk of having or developing a cardiovascular disease and/or cardiovascular event. | 2013-01-17 |
20130017558 | ADAM12 AS A BIOMARKER FOR BLADDER CANCER - The invention generally relates to isolated human or humanized antibodies or functional fragments thereof that include an antigen binding region that specifically binds a form of a disintegrin and metalloprotease 12 (ADAM12) present in a tissue or body fluid that is indicative of a cancer. | 2013-01-17 |
20130017559 | Lateral Flow Assays With Time Delayed Components - A lateral flow device may include one or more enhancement elements, where the enhancement elements bind to the analyte sandwich to increase a detection signal in the test zone. In preferred embodiments, some or all of the enhancement elements are encapsulated. | 2013-01-17 |
20130017560 | VIRAL CITRULLINATED PEPTIDES AND USES THEREOFAANM Migliorini; PaolaAACI SienaAACO ITAAGP Migliorini; Paola Siena ITAANM Pratesi; FedericoAACI SienaAACO ITAAGP Pratesi; Federico Siena IT - The present invention relates to an antigenically effective peptide comprising, from the amino to the carboxylic terminal, the amino acid sequence: G P P W W P P I C D P P Q P S K T Q G Q S X | 2013-01-17 |
20130017561 | Lateral Flow Device for Diagnosing Microbial Infections - Fungal infections are difficult to diagnose. The most common filamentous fungal infection, aspergillosis, carries with it a high mortality. Culture of the organism is difficult and obtaining samples, e.g., though a lung biopsy, sometimes causes morbidity. Biomarkers that indicate ‘early’ infection in it development are sought after. One such biomarker is detection of galactomannan (GM), a polysaccharide that is attached to hyphal cell walls and secreted during growth of the organism. Galactomannan is excreted in urine. Disclosed herein is a lateral flow assay comprising monoclonal antibodies that recognize specific residues of | 2013-01-17 |
20130017562 | Assay for Vascular CalcificationAANM Shanahan; Catherine M.AACI LondonAACO GBAAGP Shanahan; Catherine M. London GBAANM Kapustin; Alexander N.AACI LondonAACO GBAAGP Kapustin; Alexander N. London GB - An assay for identifying an individual having or at risk of developing vascular calcification, said assay comprising obtaining a blood sample from an individual and measuring the level of a vesicular compound in a matrix vesicle present in the blood sample from said individual; wherein an increased level of said compound indicates an individual at risk of developing vascular calcification. | 2013-01-17 |
20130017563 | Diagnosis of Gluten-Induced Autoimmune DiseasesAANM Korponay-Szabo; IlmaAACI BudapestAACO HUAAGP Korponay-Szabo; Ilma Budapest HUAANM Fesus; LaszloAACI DebrecenAACO HUAAGP Fesus; Laszlo Debrecen HUAANM Bagossi; PeterAACI DebrecenAACO HUAAGP Bagossi; Peter Debrecen HUAANM Csosz; EvaAACI DebrecenAACO HUAAGP Csosz; Eva Debrecen HUAANM Kiraly; RobertAACI DebrecenAACO HUAAGP Kiraly; Robert Debrecen HUAANM Simon-Vecsei; ZsofiaAACI DebrecenAACO HUAAGP Simon-Vecsei; Zsofia Debrecen HUAANM Maki; MarkkuAACI TampereAACO FIAAGP Maki; Markku Tampere FIAANM Bagossi; PeterneAACI DebrecenAACO HUAAGP Bagossi; Peterne Debrecen HU - The invention relates to selective diagnosis of gluten-induced autoimmune diseases by binding assays utilizing the main celiac epitope present on proteins of the transglutaminase family. | 2013-01-17 |
20130017564 | BIOPRINTING STATION, ASSEMBLY COMPRISING SUCH BIOPRINTING STATION AND BIOPRINTING METHODAANM Guillemot; FabienAACI BordeauxAACO FRAAGP Guillemot; Fabien Bordeaux FRAANM Catros; SylvainAACI BordeauxAACO FRAAGP Catros; Sylvain Bordeaux FRAANM Keriquel; VirginieAACI BordeauxAACO FRAAGP Keriquel; Virginie Bordeaux FRAANM Fricain; Jean-ChristopheAACI BordeauxAACO FRAAGP Fricain; Jean-Christophe Bordeaux FR - Bioprinting station (1) comprising:—a Bioprinting device (4) adapted to deposit a pattern of biological material (2) onto an area of interest (3 | 2013-01-17 |
20130017565 | METHODS AND PRODUCTS RELATED TO LOW MOLECULAR WEIGHT HEPARIN - The invention relates to methods and products for characterizing and using polysaccharides. Low molecular weight heparin products and methods of use are described. Methods for characterizing purity and activity of polysaccharide preparations including glycosaminoglycans such as heparin are also described. | 2013-01-17 |
20130017566 | METHOD FOR ASSESSING THE CONDITION OF SKIN AND/OR SCALPAANM KOSAGA; MasaruAACI SingaporeAACO SGAAGP KOSAGA; Masaru Singapore SGAANM HE; ShanAACI BeijingAACO CNAAGP HE; Shan Beijing CNAANM GONG; TianGuiAACI GuangzhouAACO CNAAGP GONG; TianGui Guangzhou CN - Method for assessing condition of skin and/or scalp by resulted color from the interaction of protein detecting composition with skin and/or scalp sample. Also disclosed is a method for comparing condition of different skin and/or scalp using the above method. Such conditions include, for example, skin healthiness and dandruff condition of scalp. | 2013-01-17 |
20130017567 | ZINC OXIDE-BASED NANOSTRUCTURE MODIFIED QCM FOR DYNAMIC MONITORING OF CELL ADHESION AND PROLIFERATION - A dynamic and noninvasive method of monitoring the adhesion and proliferation of biological cells through multimode operation (acoustic and optical) using a ZnO nanostructure-modified quartz crystal microbalance (ZnO | 2013-01-17 |
20130017568 | Inoculation Loop Devices Having an Angled End and Methods of Making and Using the Same - Inoculation loop devices having an angled end are disclosed. Methods of making and using inoculation loop devices having an angled end are also disclosed. | 2013-01-17 |
20130017569 | CULTURE MEDIUM, METHOD FOR CULTURING LISTERIA, AND METHOD FOR DETECTING LISTERIA - There are disclosed a culture media and culture supplements for cultivating microorganisms, including | 2013-01-17 |
20130017570 | METHOD FOR MONITORING STATE OF DIFFERENTIATION IN STEM CELL - A method for monitoring the differentiation state of a stem cell, includes a step (A-1) of culturing a stem cell into which a fusion gene of a promoter region of a differentiation state detection marker gene and a luminescent protein-coding gene is introduced, a step (A-2) of culturing the stem cell under a differentiation-inducing condition after the step (A-1), and a step (A-3) of capturing images of luminescence emitted by expression of the luminescent protein-coding gene in the stem cell, over at least a given period of the steps (A-1) to (A-2). | 2013-01-17 |
20130017571 | Pullulanase Variants and Uses Thereof - A truncated pullulanase variant of a parent pullulanase belonging to family GH57 comprising an X47 domain and the use thereof. | 2013-01-17 |
20130017572 | CODON MODIFIED POLYNUCLEOTIDE SEQUENCES FOR ENHANCED EXPRESSION IN A HOST SYSTEM - Synthetic DNA molecules encoding various HPV proteins are provided. The codons of the synthetic molecules are designed so as to use the codons that preferentially increase expression of the polypeptide in the host cell, which in preferred embodiments is a human cell. The codons are modified in order to minimize, decrease or eliminate cellular destruction of the polypeptide construct. | 2013-01-17 |
20130017573 | PREPARATION OF PROTECTIVE ANTIGEN - A polynucleotide sequence is provided comprising a nucleic acid sequence encoding recombinant Protective Antigen (rPA). | 2013-01-17 |
20130017574 | METHODS AND COMPOSITIONS FOR ENHANCED EXPRESSION AND SECRETION OF PROTEINS - Optimized signal peptide coding sequences for enhanced expression and secretion of protein from a cell and related compositions and methods are described. The optimized signal peptide coding sequence encodes an mRNA that contains at least one hairpin structure immediately downstream of the initiation codon. Methods for obtaining the optimized signal peptide coding sequences and methods for enhanced expression and secretion of proteins using the optimized signal peptide coding sequences are also described. | 2013-01-17 |
20130017575 | Nucleic acids encoding anti-IL-6 antibodies of defined epitopic specificity - The invention relates to nucleic acids encoding antibody or antibody fragments that specifically binds to an epitope on an intact human IL-6 polypeptide, which epitope when ascertained by epitopic mapping includes one or more residues comprised in each of the following IL-6 fragments (i) a fragment consisting of amino acid residues 37-51 of human Il-6, (ii) a fragment consisting of amino acid residues 70-84 of human Il-6, (iii) a fragment consisting of amino acid residues 169-183 of human Il-6, (iv) a fragment consisting of amino acid residues 31-45 of human Il-6 and (v) a fragment consisting of amino acid residues 58-72 of human IL-6. | 2013-01-17 |
20130017576 | METHOD FOR THE PRODUCTION OF PROTEINS AND PEPTIDESAANM Merk; HelmutAACI BerlinAACO DEAAGP Merk; Helmut Berlin DEAANM Stiege; WolfgangAACI BerlinAACO DEAAGP Stiege; Wolfgang Berlin DEAANM Gless; ChristineAACI BerlinAACO DEAAGP Gless; Christine Berlin DEAANM GERRITS; MichaelAACI BerlinAACO DEAAGP GERRITS; Michael Berlin DE - The invention relates to a method for producing monomeric or dimeric proteins or peptides containing internal or external disulfide bonds, comprising the following steps: a) a cell-free lysate, obtainable from eukaryotic cells, is provided, which contains functional microsomal vesicles, b) a nucleic acid coding the protein or peptide and additionally containing a signal sequence is added to the lysate, c) the lysate with the nucleic acid is held for a given time at a temperature in the range from 20 to 35° C., proteins or peptides formed with the nucleic acid being translocated into the microsomal vesicles, d) the microsomal vesicles are then dissolved, and the proteins or peptides obtained thereby are optionally separated from the lysate. | 2013-01-17 |
20130017577 | METHODS FOR ENHANCED PROTEIN PRODUCTION - The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein (e.g., the growth phase) in a perfusion cell culture to a high cell density (i.e., at least above about 40×10 | 2013-01-17 |
20130017578 | MUTEINS OF THE BACTERIOPHAGE LAMBDA INTEGRASESAANM Ghadessy; Farid JohnAACI SingaporeAACO GBAAGP Ghadessy; Farid John Singapore GBAANM Tay; Mei Sian YvonneAACI SingaporeAACO SGAAGP Tay; Mei Sian Yvonne Singapore SGAANM Drodge; PeterAACI SingaporeAACO SGAAGP Drodge; Peter Singapore SG - The present invention refers to muteins of the bacteriophage lambda integrases and to nucleic acid molecules comprising a nucleotide sequence encoding such muteins of the lambda integrases. The invention further refers to host cells containing a nucleic acid molecule comprising a nucleotide sequence encoding such muteins of the lambda integrases. The invention also refers to methods of recombining nucleic acids of interest into target nucleic acids in the presence of the muteins of the lambda integrases, as well as sequence specific recombination kits. | 2013-01-17 |
20130017579 | BIPHASIC SUPERCRITICAL CARBON DIOXIDE-WATER PRETREATMENT OF LIGNOCELLULOSIC BIOMASS - A process for pretreatment of a biomass material, comprising subjecting a biomass material to a biphasic mixture of water and supercritical CO | 2013-01-17 |
20130017580 | Synthesis of Prazole Compounds - The present disclosure relates to non-naturally occurring monooxygenase polypeptides useful for preparing prazole compounds, polynucleotides encoding the polypeptides, and methods of using the polypeptides. | 2013-01-17 |
20130017581 | NUCLEIC ACID CONSTRUCT COMPRISING PYRIPYROPENE BIOSYNTHETIC GENE CLUSTER AND MARKER GENEAANM Aihara; SatoAACI Odawara-ShiAACO JPAAGP Aihara; Sato Odawara-Shi JPAANM Sumida; NaomiAACI Odawara-ShiAACO JPAAGP Sumida; Naomi Odawara-Shi JPAANM Murashima; KoichiroAACI Odawara-ShiAACO JPAAGP Murashima; Koichiro Odawara-Shi JPAANM Yanai; KoujiAACI Odawara-ShiAACO JPAAGP Yanai; Kouji Odawara-Shi JPAANM Anzai; HiroyukiAACI Inashiki-GunAACO JPAAGP Anzai; Hiroyuki Inashiki-Gun JPAANM Yamamoto; KentaroAACI Kawasaki-ShiAACO JPAAGP Yamamoto; Kentaro Kawasaki-Shi JP - There is provided a nucleic acid construct comprising a pyripyropene biosynthetic gene cluster and a marker gene. The nucleic acid construct according to the present invention provides an inexpensive and highly productive method for producing pyripyropene. | 2013-01-17 |
20130017582 | ENHANCED PRODUCTION OF TAXOL AND TAXANES BY CELL CULTURES OF TAXUS SPECIES - This invention provides methods whereby taxol, baccatin III, and other taxol-like compounds, or taxanes, can be produced in very high yield from all known | 2013-01-17 |
20130017583 | METHODS FOR PRODUCING POLYHYDROXYALKANOATE COPOLYMER WITH HIGH MEDIUM CHAIN LENGTH MONOMER CONTENT - The invention relates to the production of polyhydroxyalkanoate copolymer with high 3-hydroxyhexanoate monomer content through recombinant gene expression. | 2013-01-17 |
20130017584 | Enzymatic Hydroxylation of Aliphatic HydrocarbonAANM Hofrichter; MartinAACI DresdenAACO DEAAGP Hofrichter; Martin Dresden DEAANM Scheibner; KatrinAACI JenaAACO DEAAGP Scheibner; Katrin Jena DEAANM Ullrich; ReneAACI ZittauAACO DEAAGP Ullrich; Rene Zittau DEAANM Kinne; MatthiasAACI GorlitzAACO DEAAGP Kinne; Matthias Gorlitz DEAANM Peter; SebastianAACI ZittauAACO DEAAGP Peter; Sebastian Zittau DEAANM Lund; HenrikAACI VaerloeseAACO DKAAGP Lund; Henrik Vaerloese DKAANM Kalum; LisbethAACI VaerloeseAACO DKAAGP Kalum; Lisbeth Vaerloese DK - The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of substituted or unsubstituted, linear or branched aliphatic hydrocarbons. | 2013-01-17 |
20130017585 | PROCESS FOR THE PRODUCTION OF ALCOHOLS AND/OR SOLVENTS FROM PAPERMAKING PULPS WITH RECYCLING OF NON-HYDROLYZED VEGETATIONAANM Ropars; MarcelAACI PalaiseauAACO FRAAGP Ropars; Marcel Palaiseau FRAANM Aymard; CarolineAACI LyonAACO FRAAGP Aymard; Caroline Lyon FR - This invention describes a process for the production of alcohols and/or solvents from cellulosic or lignocellulosic biomass that comprises at least the following stages:
| 2013-01-17 |
20130017586 | METHOD FOR PRODUCING ALCOHOLS AND/OR SOLVENTS FROM PAPER PULPS WITH RECYCLING OF THE NON-HYDROLYSATED PLANT MATERIAL IN A REGENERATION REACTORAANM Ropars; MarcelAACI PalaiseauAACO FRAAGP Ropars; Marcel Palaiseau FRAANM Aymard; CarolineAACI LyonAACO FRAAGP Aymard; Caroline Lyon FRAANM Guillaume; AnaisAACI Rueil-MalmaisonAACO FRAAGP Guillaume; Anais Rueil-Malmaison FRAANM Menir; SandraAACI GonesseAACO FRAAGP Menir; Sandra Gonesse FR - This invention describes a process for the production of alcohols and/or solvents from cellulosic or lignocellulosic biomass that comprises at least the following stages:
| 2013-01-17 |
20130017587 | Method for Recovery of Organic Components from Dilute Aqueous SolutionsAANM Nierlich; FranzAACI MarlAACO DEAAGP Nierlich; Franz Marl DEAANM Burger-Kley; WalterAACI KlagenfurtAACO ATAAGP Burger-Kley; Walter Klagenfurt ATAANM Mikenberg; IljaAACI BochumAACO DEAAGP Mikenberg; Ilja Bochum DEAANM Kneissel; BernhardAACI BonnAACO DEAAGP Kneissel; Bernhard Bonn DE - The present invention relates to a method for recovering an organic component from an aqueous medium such as a fermentation broth containing microorganism producing said organic component. The method includes increasing the activity of the organic component in the aqueous medium by increasing the concentration of at least one hydrophilic solute in the medium leading to salting-out of the organic component. The microorganisms are genetically modified to be capable of tolerating higher concentrations in the medium in comparison to their unmodified counterparts. | 2013-01-17 |
20130017588 | RECOMBINANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE BUTANOL AND METHOD OF PRODUCING BUTANOL USING THE SAMEAANM Lee; Sang YupAACI DaejeonAACO KRAAGP Lee; Sang Yup Daejeon KRAANM Jang; Yu-SinAACI DaejeonAACO KRAAGP Jang; Yu-Sin Daejeon KRAANM Lee; Jin YoungAACI IncheonAACO KRAAGP Lee; Jin Young Incheon KRAANM Lee; Joung MinAACI Gangwon-doAACO KRAAGP Lee; Joung Min Gangwon-do KRAANM Park; Jin HwanAACI DaejeonAACO KRAAGP Park; Jin Hwan Daejeon KR - The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels. | 2013-01-17 |
20130017589 | FRACTIONATION OF LIGNOCELLULOSIC BIOMASS FOR CELLULOSIC ETHANOL AND CHEMICAL PRODUCTION - A process is defined for the continuous steam pretreatment and fractionation of corn cobs and low lignin lignocellulosic biomass to produce a concentrated cellulose solid stream that is sensitive to enzymatic hydrolysis. Valuable chemicals are recovered by fractionating the liquid and vapor stream composed of hydrolysis and degradation products of the hemicellulose. Cellulosic derived glucose is produced for fermentation to biofuels. A hemicellulose concentrate is recovered that can be converted to value added products including ethanol. | 2013-01-17 |
20130017590 | PREPARATION METHOD OF TRANSPORTATION FUEL OR LUBRICATING BASE OIL USING BIOMASSAANM Chung; Young MinAACI Yuseong-guAACO KRAAGP Chung; Young Min Yuseong-gu KRAANM Kim; Ok YounAACI Yuseong-guAACO KRAAGP Kim; Ok Youn Yuseong-gu KRAANM Jeon; Hee JungAACI Yuseong-guAACO KRAAGP Jeon; Hee Jung Yuseong-gu KRAANM Yoon; Young SeekAACI Buk-guAACO KRAAGP Yoon; Young Seek Buk-gu KRAANM Lee; Seong HoAACI Gangseo-guAACO KRAAGP Lee; Seong Ho Gangseo-gu KRAANM Kim; Hee SooAACI Yuseong-guAACO KRAAGP Kim; Hee Soo Yuseong-gu KRAANM Oh; Seung HoonAACI Gangnam-guAACO KRAAGP Oh; Seung Hoon Gangnam-gu KRAANM Yim; Yoon JaeAACI Yeongi-gunAACO KRAAGP Yim; Yoon Jae Yeongi-gun KR - The present invention relates to a method of economically preparing paraffin compounds corresponding to gasoline fuel or lubricating base oil using volatile fatty acids (VFAs) derived from biomass. | 2013-01-17 |
20130017591 | Method for the Preparation of Recombinant Human Thrombin and Fibrinogen - The present invention discloses methods for the preparation of recombinant human proteins expressed in human cells. The present invention relates to methods for the preparation of human recombinant thrombin and human recombinant fibrinogen. The method employs serum-free culturing conditions and provides recombinant human proteins expressed in human cells of increased safety to the patient when used in human medical treatments. The immunogenic response to the recombinant human proteins expressed in human cells may be lower. Human recombinant thrombin is expressed in the human embryonic kidney 293 cell line and the protein can be prepared using two different routes, one starting from a point mutated prothrombin with gla and Kringle 1 and 2 domains, and maintaining these domains during the process; the other one starting with prothrombin (non-mutated), via a prethrombin with a HPC4-Kringle 2 domain and subjecting this prethrombin to a point mutation. | 2013-01-17 |
20130017592 | CRYSTAL METABOLITE RECOVERYAANM Pind; Peter FrodeAACI HerlevAACO DKAAGP Pind; Peter Frode Herlev DKAANM Glanville; SimonAACI KalundborgAACO DKAAGP Glanville; Simon Kalundborg DKAANM Jakobsen; SuneAACI VaerloeseAACO DKAAGP Jakobsen; Sune Vaerloese DKAANM Kaasgaard; SvendAACI SkovlundeAACO DKAAGP Kaasgaard; Svend Skovlunde DK - A method for producing a crystalline and/or amorphous metabolite suspension from a cell fermentation broth in a continuous centrifuge process comprising (a) adding at a separate inlet to the centrifuge the fermentation broth comprising the cells and the metabolite of interest, wherein the metabolite is partly or wholly on crystalline and/or amorphous form; (b) adding at another inlet to the centrifuge an aqueous liquid comprising a salt and/or a carbohydrate having a higher density than the cells and a lower density than the metabolite of interest in its precipitated form; (c) removing the cells at a separate outlet to the centrifuge; and (d) removing the suspension comprising the crystalline and/or amorphous metabolite of interest at another outlet to the centrifuge. | 2013-01-17 |
20130017593 | Biological Synthesis of Difunctional Hexanes and Pentanes from Carbohydrate Feedstocks - Provided herein are methods for the production of difunctional alkanes in microorganisms. Also provided are enzymes and nucleic acids encoding such enzymes, associated with the difunctional alkane production from carbohydrates feedstocks in microorganisms. The invention also provides recombinant microorganisms and metabolic pathways for the production of difunctional alkanes. | 2013-01-17 |
20130017594 | ALGAL LIPID COMPOSITIONS AND METHODS OF PREPARING AND UTILIZING THE SAME - This invention relates to compositions comprising high lipid content algae and methods of making and utilizing the same. In particular, the invention relates to high lipid content algae biomass and algal lipid materials derived from the same, methods of making the same, as well as to biofuels (e.g., biodiesel) and dietary compositions (e.g., animal feeds) comprising or made from the same. Compositions and methods of the invention find use in a variety of applications including biofuel, dietary (e.g., human and animal nutrition), therapeutic as well as research applications. | 2013-01-17 |
20130017595 | ANALYTICAL APPARATUS AND DETECTION METHOD EMPLOYED IN ANALYTICAL APPARATUSAANM Yamamoto; ShuheiAACI MitoAACO JPAAGP Yamamoto; Shuhei Mito JPAANM Sano; MinoruAACI HitachinakaAACO JPAAGP Sano; Minoru Hitachinaka JPAANM Nemoto; AkiyukiAACI MitoAACO JPAAGP Nemoto; Akiyuki Mito JPAANM Fujioka; MichiruAACI HitachinakaAACO JPAAGP Fujioka; Michiru Hitachinaka JP - Disclosed is an analytical apparatus wherein a container is automatically transferred and the container is automatically closed with a cap member, said analytical apparatus being provided with a suitable mechanism that reliably closes the container. The analytical apparatus is provided with an optical sensor composed of at least one pair of a light source ( | 2013-01-17 |
20130017596 | Polycistronic Vector for Human Induced Pluripotent Stem Cell Production - Methods of producing induced pluripotent stem (iPS) cells are provided. For example, a method of producing an iPS cell from a differentiated cell, which includes transforming the differentiated cell with a first vector comprising a nucleic acid sequence comprising a nucleic acid sequence encoding an Oct4, a nucleic acid sequence encoding a Sox2, and a nucleic acid sequence encoding a Klf4. Each of the nucleic acid sequences are separated from each other by a first and second viral 2A sequence. The method described can further comprise culturing the transformed cell under conditions that allow for the production of an iPS cell and isolating the cultured iPS cell. | 2013-01-17 |
20130017597 | USE OF BIOLOGICAL PHOTORECEPTORS AS DIRECTLY LIGHT-ACTIVATED ION CHANNELS - Use of a biological photoreceptor as light-controlled ion channel for the alteration of the ion conductivity of a membrane by means of light. The photoreceptor used comprises an apoprotein and a light-sensitive polyene covalently bound to the apoprotein, said polyene interacting with the apoprotein and functioning as a light-sensitive gate. | 2013-01-17 |
20130017598 | PREVENTION OF DISULFIDE BOND REDUCTION DURING RECOMBINANT PRODUCTION OF POLYPEPTIDES - The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures. | 2013-01-17 |
20130017599 | METHOD OF DIMINISHING THE SYMPTOMS OF NEURODEGENERATIVE DISEASE - A method of diminishing the symptoms of neurodegenerative disease in a patient is disclosed. In one embodiment, the method comprises the steps of: (a) identifying a patient with a neurodegenerative disease, (b) producing a cell culture, wherein the cell culture comprises cells with induced antioxidant response element (ARE) mediated transcription, and (c) transplanting at least a portion of the cell culture into the brain of the patient, wherein symptoms of neurodegenerative disease are diminished. | 2013-01-17 |
20130017600 | ISOLATION AND/OR PRESERVATION OF DENDRITIC CELLS FOR PROSTATE CANCER IMMUNOTHERAPY - Methods and compositions for use of human dendritic cells to activate T cells for immunotherapeutic responses against primary and metastatic prostate cancer are disclosed. In one embodiment, human dendritic cells, after exposure to a prostate cancer antigen or specific antigenic peptide, are administered to a prostate cancer patient to activate the relevant T cell responses in vivo. In an alternate embodiment, human dendritic cells are exposed to a prostate cancer antigen or specific antigenic peptide in vitro and incubated or cultured with primed or unprimed T cells to activate the relevant T cell responses in vitro. The activated T cells are then administered to a prostate cancer patient. Methods and compositions for human dendritic cells with extended life span and cryopreserved dendritic cells are disclosed. | 2013-01-17 |
20130017601 | MODULATION OF PLASMA MEMBRANE HUMAN LEUKOCYTE ELASTASE - A method for modulation of plasma membrane associated Human Leukocyte Elastase (HLE) to inflammatory states by interaction of HLE with an antagonist to inhibit HLE and thereby interruption in plasma associated events (e.g. HIV disease progression, bacterial infections and autoimmune diseases), which are responsive/sensitive to such inflammation. The antagonist suitable for use in this invention is designed to interact with each of the catalytic triad of the HLE plasma membranes protein and the lipid interactive amino acids of the HLE plasma membrane protein. | 2013-01-17 |
20130017602 | MODULATION OF OOCYTE MEIOTIC PROGRESSION AND OOCYTE ACTIVATION - The present invention provides methods, compositions, and systems for reinitiating meiosis in cells in meiotic arrest and oocyte activation in fertilized, but un-activated, oocytes. In certain embodiments, Zn-binding moieties (e.g., zinc chelators) are used for reinitiating meiosis or oocyte activation. | 2013-01-17 |
20130017603 | GLUTAMATE DERIVATIVES OR SALTS THEREOF - Compounds having an excellent CaSR agonist activity are in demand. The invention provides glutamate derivatives or salts thereof, pharmaceutical compositions comprising the glutamate derivatives, preventive or therapeutic agents for diarrhea, hyperparathyroidism or peptic ulcer. | 2013-01-17 |
20130017604 | USE OF ADIPOSE TISSUE-DERIVED STROMAL CELLS FOR CHONDROCYTE DIFFERENTIATION AND CARTILAGE REPAIR - Methods and compositions for directing adipose-derived stromal cells cultivated in vitro to differentiate into cells of the chondrocyte lineage are disclosed. The invention further provides a variety of chondroinductive agents which can be used singly or in combination with other nutrient components to induce chondrogenesis in adipose-derived stromal cells either in cultivating monolayers or in a biocompatible lattice or matrix in a three-dimensional configuration. Use of the differentiated chondrocytes for the therapeutic treatment of a number of human conditions and diseases including repair of cartilage in vivo is disclosed. | 2013-01-17 |
20130017605 | ORALLY BIOAVAILABLE STILBENOIDS- COMPOSITIONS AND THERAPEUTIC APPLICATIONS THEREOF - A novel, bioavailable and safe stilbenoid 3,5-dimethoxy-3,4′-dihydroxystilbene represented by SIR#1 with an unexpected enhanced ability to prevent the accumulation of lipids accompanying the terminal differentiation of adipocytes, thereby inhibiting adipogenesis, and nutraceutical and cosmeceutical compositions comprising, dimethoxy-3,4′-dihydroxystilbene useful for anti-obesity and anti-cellulite therapy, are disclosed. Further the enhanced SIRT-1 activation ability of 3,5-dimethoxy-3,4′-dihydroxystilbene represented by STR#1 and 2,3′,5′,6-tetrahydroxy-trans-stilbene represented by STR#II are disclosed. The enhancement of SIRT-1 polypeptide activity of the said compounds is unexpectedly much higher than resveratrol or its natural analog pterostilbene. Sirtuin modulating compositions comprising an orally bioavailable SIRT-1 enhancing compounds (i) 3,5-dimethoxy-3,4′-dihydroxystilbene represented by STR#I and (ii) 2,3′,5′,6-tetrahydroxy-trans-stilbene represented by are also disclosed. An additional embodiment, also discloses the enhanced anti- | 2013-01-17 |
20130017606 | METHOD OF SEPARATING PANCREATIC ISLET - The present invention provides a pancreatic islet isolation method comprising the steps of (1) injecting a protection solution containing a protease inhibitor into the pancreatic duct of an procured pancreas; (3) digesting the pancreas into which the protection solution has been injected; and (4) purifying the digested pancreatic tissue using a purification solution containing a density gradient reagent. The present invention also provides a protection solution for injection into the pancreatic duct, a pancreas preservation solution for the two-layer method, and an islet purification solution. | 2013-01-17 |
20130017607 | Nanotube carrier substrate for primary tissue cultureAANM Mayr; StefanAACI LeipzigAACO DEAAGP Mayr; Stefan Leipzig DEAANM Zink; MareikeAACI LeipzigAACO DEAAGP Zink; Mareike Leipzig DEAANM Dallacasagrande; ValentinaAACI LeipzigAACO DEAAGP Dallacasagrande; Valentina Leipzig DEAANM Kas; JosefAACI LeipzigAACO DEAAGP Kas; Josef Leipzig DEAANM Reichenbach; AndreasAACI LeipzigAACO DEAAGP Reichenbach; Andreas Leipzig DE - A carrier substrate for primary tissue culture has a nanotube array. A tissue culture vessel has an outer vessel and a nanotube carrier substrate with a nanotube array, located within the outer vessel, wherein the surface roughness of the nanotube array is 1 nm to 100 nm. The nanotube array is used for in vitro culturing of primary tissue in connection with a tissue culture vessel for in vitro culturing of primary tissue and a method for in vitro culturing primary tissue, wherein a nanotube array is arranged essentially horizontal inside an outer cell culture vessel, so that openings of the nanotubes point at least in upward direction, the nanotube array is contacted with cell culture medium and an isolated primary tissue sample is placed on top-side on said nanotube array. | 2013-01-17 |
20130017608 | METHODS OF INCREASING YIELDS OF PLEUROMUTILINS - This invention relates to a novel Pleuromutilin gene cluster and methods of increasing yields of Pleuromutilin produced by | 2013-01-17 |
20130017609 | APPARATUS AND METHOD FOR INDICATING A PHYSICAL OR CHEMICAL PHENOMENONAANM Kansakoski; MarkkuAACI OuluAACO FIAAGP Kansakoski; Markku Oulu FIAANM Hurme; EeroAACI EspooAACO FIAAGP Hurme; Eero Espoo FIAANM Hakalahti; LeenaAACI OuluAACO FIAAGP Hakalahti; Leena Oulu FIAANM Korhonen; RaimoAACI TampereAACO FIAAGP Korhonen; Raimo Tampere FIAANM Kemppainen; AnttiAACI OuluAACO FIAAGP Kemppainen; Antti Oulu FI - The invention relates to an apparatus ( | 2013-01-17 |
20130017610 | ENCAPSULATED TRACERS AND CHEMICALS FOR RESERVOIR INTERROGATION AND MANIPULATION - An apparatus, method, and system of reservoir interrogation. A tracer is encapsulating in a receptacle. The receptacle containing the tracer is injected into the reservoir. The tracer is analyzed for reservoir interrogation. | 2013-01-17 |
20130017611 | SENSOR MATERIAL AND METHOD FOR FABRICATING THE SAME AND DETECTING METHODAANM LI; Shou-NanAACI Nantou CountyAACO TWAAGP LI; Shou-Nan Nantou County TWAANM LIN; Hong-PingAACI Taipei CityAACO TWAAGP LIN; Hong-Ping Taipei City TWAANM SHIH; Hui-YaAACI Changhua CountyAACO TWAAGP SHIH; Hui-Ya Changhua County TWAANM HSIEH; Wen-AnAACI New Taipei CityAACO TWAAGP HSIEH; Wen-An New Taipei City TWAANM LIN; Jhih-JieAACI New Taipei CityAACO TWAAGP LIN; Jhih-Jie New Taipei City TW - A method for fabricating a sensor material for detecting molecular contaminants, including the steps of: providing an aqueous solution of a metal oxide precursor; mixing titanium dioxide nanotubes with the aqueous solution of the metal oxide to form a mixture; adjusting a pH value of the mixture with a weak base until the pH value is neutral; dispersing the mixture in water and heating the mixture; and filtering the mixture to retain a solid part, and calcining the solid part under a continuous flow of oxygen to form metal oxide loaded titanium dioxide nanotubes. The disclosure also provides a sensor material and a detecting method using the sensor material for ppm-ppb-ppt concentration level detection of molecular contaminants. | 2013-01-17 |