Class / Patent application number | Description | Number of patent applications / Date published |
530344000 | Separation or purification | 56 |
20080207876 | Triazines and Pyrimidines as Protein Binding Ligands - Compounds of the general formula (I): in which inter alia Q | 08-28-2008 |
20080275216 | Fluorous Capping Reagents and Methods for Peptide Purification - Aspects of the present invention relate to compounds for preparing fluorocarbon compounds, methods for preparing fluorocarbon compounds, and methods for purifying a mixture of compounds. One aspect of the present invention relates to a trivalent iodonium fluorocarbon. The trivalent iodonium fluorocarbon compound of the invention is useful for attaching a fluorocarbon group to a compound that has a nucleophilic functional group. Another aspect of the present invention relates to a method of preparing a trivalent iodonium fluorocarbon. Another aspect of the present invention relates to a method of preparing a fluorocarbon by treating a compound bearing a nucleophilic functional group with a trivalent iodonium fluorocarbon compound. Another aspect of the present invention relates to a method or purifying a mixture comprising a first and a second compound by treating the mixture with a trivalent iodonium fluorocarbon to attach a fluorocarbon group to the second compound leaving the first compound unchanged, and purifying the mixture by fluorous-phase purification. | 11-06-2008 |
20080281078 | Ion exchange chromatography of GLP-1, analogs and derivatives thereof - The present invention relates to an ion exchange chromatography process for purifying GLP-1 or an analog or a derivative thereof from a mixture containing said GLP-1 and related impurities, and to an industrial method including such ion exchange chromatography process. | 11-13-2008 |
20080312411 | Use of Activated Polymers for Separation of Protein and Polypeptide Multimers - The invention relates to a use of an activated polymer to separate a non-covalently associated polypeptide multimer comprising multiple polypeptide subunits into multiple polypeptide subunits. | 12-18-2008 |
20090012265 | Purification of polymers carrying a lipophilic group - This invention relates to a method of producing a purified polymer selected from the group consisting of PNAs, (poly)peptides, PNA chimera, peptide-DNA chimera, and derivatives thereof, wherein the polymer carries at least one lipophilic group and wherein the method comprises the following steps: (a) transferring a solution comprising the polymer carrying a lipophilic group to a lipophilic surface under conditions that allow binding of said lipophilic group to said lipophilic surface; (b) washing said surface under conditions that allow said binding to be maintained, wherein polymers not carrying said lipophilic group are removed; (c) washing said surface under conditions that break said binding; (d) collecting the washing solution from step (c); and (e) obtaining said purified polymer from said washing solution. Preferably, the lipophilic group is a protection group, or a label such as a fluorescent dye, or a linker, for example a linker suitable for immobilization on a support. | 01-08-2009 |
20090023897 | METHOD AND APPARATUS FOR PURIFYING AND DESALTING BIOLOGICAL SAMPLES - The subject invention provides a sample processing technique for purifying a biological or chemical sample. The invention is particularly well-suited to prepare a sample for mass spectrometry. The process is to be performed in an article having at least one well, in which the surface of the well is at least partially hydrophobic and/or modified with bio-specific ligands. Targeted solutes, such as salts or small molecule contaminants, can be removed from a solution to allow for a purified solution of a desired type of solute, such as peptides and/or proteins. | 01-22-2009 |
20090023898 | METHODS AND COMPOSITIONS FOR PROTEIN PURIFICATION - The present invention provides methods of purifying proteins that include a metal ion affinity peptide. The methods generally involve contacting a fusion protein that includes a metal ion affinity peptide with at least two different metal ion chelating resins. In certain representative embodiments, the methods include contacting a fusion protein with a first metal ion chelate resin having a first immobilized metal ion; eluting any bound protein from the first metal ion chelate resin, to produce an eluate; contacting the eluate with a second metal ion chelate resin having a second immobilized metal ion; and eluting any bound protein from the second metal ion chelate resin. Also provided are kits for use in practicing the subject methods. The subject methods find use in a variety of protein purification applications. | 01-22-2009 |
20090043075 | RECOMBINANT PEPTIDE PRODUCTION USING A CROSS-LINKABLE SOLUBILITY TAG - The invention relates to the recombinant expression of a peptide of interest in the form of a fusion protein comprising a solubility tag. The fusion protein comprises at least two portions separated by a cleavable peptide sequence wherein one portion is devoid of cysteine residues and the second portion comprises an effective number of cross-linkable cysteine residues. After cell lysis and isolation of the fusion protein, the fusion protein is subsequently cleaved into a mixture of first and second portions. Oxidative cross-linking is used to selectively precipitate one of the two portions to facilitate simple and effective separation of the peptide of interest. | 02-12-2009 |
20090105450 | Use of modified metal oxides for enrichment of phosphopeptides - Use of modified metal oxides for the purification and enrichment of negatively charged biomolecules such as peptides, proteins, DNA, RNA, Lipids, carbohydrates, glyco molecules. These metal oxides are modified in such a way that the density of the Lewis acid group is reduced due to modification. | 04-23-2009 |
20090105451 | Use of aqueous wettable hydrophobic chromatographic media for the purification of peptides, and other biomolecules - The use of hydrophobic coated metal oxides particles or film for the purifications of proteins, peptides or other biomolecules. This process eliminates two steps of purification as compared to silica or polymer based particles. | 04-23-2009 |
20090182120 | SURFACE MEDIATED SELF-ASSEMBLY OF NANOPARTICLES - Materials and methods for surface mediated self assembly of nanoparticles for the isolation of biomolecules is provided. | 07-16-2009 |
20090187005 | ENHANCED PURIFICATION OF ANTIBODIES AND ANTIBODY FRAGMENTS BY APATITE CHROMATOGRAPHY - Methods are disclosed for use of apatite chromatography, particularly without reliance upon phosphate gradients, for purification or separation of at least one intact non-aggregated antibody, or at least one immunoreactive antibody fragment, from an impure preparation. Integration of such methods into multi-step procedures with other fractionation methods are additionally disclosed. | 07-23-2009 |
20090203880 | Production of Canola Protein - A canola protein isolate having a protein content of at least about 90 wt % (N×6.25), preferably at least about 100 wt %, and consisting predominantly of the 2S protein and substantially free from the 7S and 12S proteins is prepared. In one aspect, canola oil seed meal is extracted with aqueous protein solution at an elevated temperature to preferentially extract 2S protein from the meal to produce a canola protein solution containing predominantly 2S protein. The 2S canola protein is recovered as an isolate. In another aspect, canola oil seed meal is initially extracted with water to preferentially extract 7S and 12S canola proteins followed by extraction of the canola oil seed meal with aqueous saline solution to extract 2S protein from the meal. 2S canola protein isolate is recovered from the saline extract. In another aspect, the canola oil seed meal is extracted with aqueous saline solution to extract 2S, 7S and 12S proteins from the meal. The aqueous protein extract solution is heat treated at an elevated temperature to precipitate 7S and 12S proteins and leave a 2S protein solution from which the isolate may be recovered. In a further aspect, the aqueous protein solution is concentrated prior to the heat treatment. | 08-13-2009 |
20090203881 | Polymyxin B Analogs for LPS Detoxification - The invention relates to SAEP II peptide dimers that mimic polymyxin B i.a. in its ability to bind non-covalently the lipopolysaccharide (LPS) of Gram-negative bacteria with high affinity, and therefore to detoxify LPS as polymyxin B does. The dimeric structure is maintained by a pair of disulphide bonds involving the two cystein residues present in the peptide sequence, which does not exceed 17 amino acids and essentially comprises cationic and hydrophobic amino acid residues. In the dimers of the invention, peptides may have a parallel or anti-parallel orientation. As a matter of example, a dimer of the invention is constituted by a peptide of formula NH2-Lys-Thr-Lys-Cys1-Lys-Phe-Leu-Leu-Leu-Cys2-COOH, either in a parallel or antiparallel dimeric form. SAEP II dimers are useful for treating or preventing septic shock and related disorders generated by Gram-negative bacteria infection. The invention also relates to LPS-peptide complexes in which LPS and SAEP II dimers are non-covalently bound together. These complexes are useful as vaccinal agents against Gram-negative bacteria infection. | 08-13-2009 |
20090234099 | Purification of Peptides from Colostrum - The invention relates to the purification of peptides from colostrum. The method involves the addition of an alcohol such as methanol or ethanol to the mixture in order to form an alcohol phase rich in the peptides, and a precipitate. The peptide-rich alcohol phase is subsequently recovered and subjected to further fractionation. The invention is particularly useful in the purification of colostrinin from colostrum. | 09-17-2009 |
20090240031 | Process and intermediate products for preparing cardiodilatin fragments, and highly purified cardiodilatin fragments - The invention relates to a process for the preparation of cardiodilatin fragments, to highly purified cardiodilatin fragments, and to appropriate intermediates for the preparation of said fragments. Furthermore, the invention relates to highly purified cardiodilatin fragments which are free of peptide impurities and exhibit a single migration peak in capillary electrophoresis, as well as to appropriate processes for the preparation of same. | 09-24-2009 |
20090240032 | Novel sugar-immobilized metal nanoparticle, method for measuring sugar-protein interaction using the same and method for recovering protein from sugar-protein interactant - It is intended to provide a stable novel sugar-immobilized metal nanoparticle capable of easily immobilizing a sugar chain, a method for measuring sugar-protein interaction easily and at a low cost using the same without labeling, and a method for simply recovering a protein from a sugar-protein interactant. A maltose-immobilized gold nanoparticle was obtained by binding a ligand complex, in which maltose and a linker compound had been bound to each other, to a gold nanoparticle. By adding this maltose-immobilized gold nanoparticle to a dilution series of concanavalin A, a sugar-protein interactant of maltose and ConA was formed, and red-purple color derived from a colloidal solution of maltose-immobilized gold nanoparticle disappeared. That is, sugar-protein interaction could be confirmed by visual observation without labeling. | 09-24-2009 |
20090240033 | AFFINITY MATRIX LIBRARY AND ITS USE - The invention relates, at least in part, to an affinity matrix library and the construction and use thereof. The library may be used, for example, for the enrichment of low-abundance proteins and depletion of abundant proteins in the search for biologically important proteins. The present invention also relates to a synthetic affinity matrix library comprising one or more ligand compounds with groups selected from amino, sulfhydryl, hydroxyl, carbonyl, and/or active hydrogen. The ligand compound may be attached to a base matrix. | 09-24-2009 |
20090286955 | Methods of Separating Biopolymer Conjugated Molecules From Unconjugated Molecules - The invention relates to methods for separating or purifying biopolymer conjugated molecules from unconjugated molecules. In particular, methods are described for purifying a PEGylated protein or oligonucleotide from an unPEGylated protein or oligonucleotide, respectively. The methods are quick and efficient separation methods because they do not require gradient chromatography, fractionation of an eluent or analysis of the eluted fractions. Further, the methods increase yield and purity of the biopolymer conjugated molecule. | 11-19-2009 |
20090292109 | Method of Isolating Biomacromolecules Using Polyalkylene Glycol and Transition Metals - The present invention is related to a method of isolating a biological macromolecule in a composition. Specifically, the present invention is related to a method of isolating a biomacromolecule in a composition containing an impurity, the method comprising adding a polyalkylene glycol to the composition, adding a transition metal to the composition, and separating said biomacromolecule from said impurity. | 11-26-2009 |
20090306341 | LC/MS Blends Containing Ionizing Additives - The invention relates to the preparation of a salt in acetonitrile, characterised in that the acid component of the salt is an organic acid which boils at less than 300° C. under normal pressure, the base component of the salt is a base which boils at less than 300° C. under normal pressure, an organic acid which boils at less than 300° C. under normal pressure is added in the quantity of up to 1 vol. %, in relation to the volume of acetonitrile, and the water content, that can be determined by Karl Fischer titration, is below 5%. | 12-10-2009 |
20090306342 | BINDING OF MOLECULES - A method of binding a target molecule from an analyte containing the target molecule in admixture with at least one non-target molecule, which method comprises exposing the analyte to a substrate comprising first and second surface binding components, wherein the first surface binding component has specific binding affinity with respect to the target molecule but is independently unable to provide effective binding of the target molecule to the substrate, wherein the second surface binding component has non-specific binding affinity with respect to the target molecule and is independently unable to provide effective specific binding of the target molecule to the substrate, and wherein the target molecule is immobilized on the substrate by the combined binding effect of the first and second surface binding components. | 12-10-2009 |
20090318670 | Separation of Proteins from Grasses Integrated with Ammonia Fiber Explosion (AFEX) Pretreatment and Cellulose Hydrolysis - A process for extracting an aqueous ammonium hydroxide solution from a plant biomass after an Ammonia Fiber Explosion (AFEX) process step, is described. The proteins can be separated before or after a hydrolysis of sugar precursors (carbohydrates) from the biomass to produce sugars for fermentation to produce ethanol. The proteins are useful as animal feeds because of their amino acid food values. | 12-24-2009 |
20100029905 | METHOD FOR PURIFYING PROTEIN - The present invention provides a method for purifying a protein, includes the step of: contacting a fusion protein of a first protein and a second protein with a bivalent cation-containing solution, the fusion protein being adsorbed to a silicon oxide-containing substance, the first protein being capable of binding to the silicon oxide-containing substance in a solution containing 0.1M sodium chloride. With this arrangement, it is possible to easily produce large quantity of proteins which are high in purity without sacrificing activity of the proteins. | 02-04-2010 |
20100048867 | DEVICE AND PROCESS FOR PURIFYING NUCLEIC ACIDS - An apparatus for purifying nucleic acids by negative chromatography is disclosed, which comprises a hollow body with an opening, respectively, at the top and at the bottom end, said hollow body containing a stationary solid phase, characterized in that the stationary phase contains at least 2 different chromatography resins, such as size e.g. exclusion gel filtration materials (SEC materials), as well as a method for purifying nucleic acids and the use of the apparatus. | 02-25-2010 |
20100087623 | Method of extracting proteins and peptides from nano pearl powder - A method of extracting proteins and peptides from nano pearl powder comprising the steps of preparing a solution containing uniformly dissolved nano pearl powder; rotating the solution to form a suspension; sifting the suspension; separating a first organic compound extract of pearl having a molecular weight more than a predetermined molecular weight and a second organic compound extract of pearl having a molecular weight less than the predetermined molecular weight from the sifted suspension respectively; and activating a first gel filter to obtain pearl proteins from the first organic compound extract of pearl, and activating a second gel filter to obtain pearl peptides from the second organic compound extract of pearl respectively. | 04-08-2010 |
20100125128 | PHOSPHOPEPTIDE ENRICHMENT OF COMPOSITIONS BY FRACTIONATION ON CERAMIC HYDROXYAPATITE - Phosphorylated peptides are extracted from digests of biological liquids and other peptide mixtures by fractionation on ceramic hydroxyapatite. The ceramic hydroxyapatite is readily usable in a centrifuge, allowing for rapid fractionations of a large number of small volume samples, and accordingly high throughput. | 05-20-2010 |
20100160605 | PACKING MATERIAL FOR LIQUID CHROMATOGRAPHY AND PROCESS FOR SEPARATION AND PURIFICATION OF BIOPOLYMER BY MEANS OF THE PACKING MATERIAL - To provide a novel packing material for liquid chromatography capable of separating and purifying, or collecting and recovering, a biopolymer such as a protein or a peptide by adsorption and desorption by a pH change without being influenced by the isoelectric point of the protein or by the salt concentration in a solvent in which the biopolymer such as the protein is dissolved, and to provide a process for concentrating and recovering a desired biopolymer such as a protein or a peptide from a large amount of dilute cell culture solution by means of such a packing material. | 06-24-2010 |
20100160606 | PROCESS FOR PURIFYING A POLYMER MIXTURE - A process of purifying copolymer peptides such as COP-1 polypeptides by ultrafiltration can be improved by treating the polypeptide solution with an acid before, or during the early stages of, the ultrafiltration. By adding the acid and/or forming an acid addition salts of the polypeptide before ultrafiltration is conducted permits for faster ultrafiltration. Likewise, adding the acid in the ultrafiltration process but before polypeptide precipitation or clogging occurs can also improve the efficiency/convenience of the ultrafiltration step. | 06-24-2010 |
20100228006 | Process for purifying lipopeptides - The present invention relates to a process for purifying lipopeptides. More particular, the invention provides an improved method for purifying daptomycin. | 09-09-2010 |
20100234567 | METHODS FOR SEPARATING RECOMBINANT PROTEINS IN AQUEOUS TWO-PHASE SYSTEMS - The object of the present invention is a procedure for the distribution, separation and purification in aqueous solution of recombinant proteins, based on the utilization of polypeptides with choline affinity. The invention is based on a phenomenon consisting of that two aqueous solutions with determinated components can be mixed, being distributed finally in two phases with different density. The fusionated proteins to said polypeptides with choline affinity are preferably located in one of the phases, while most of the cell extract proteins tend to go to the opposite phase. After a series of washings for removing the rest of the not desired material, this location can be inverted through the addition of a soluble molecule with affinity by the polypeptide fusionated to the protein of interest. This procedure allows modulating at convenience the presence of the protein or polypeptide of interest in one phase or another, possibiliting its purification with a high yield and purity grade. The invention represents an economic and scalable way of recombinant protein separation labelled preferably with choline-binding domains. | 09-16-2010 |
20100292440 | Binding Molecules for Human Factor VIII and Factor VIII-Like Proteins - It is an object of the present invention to provide novel binding molecules for factor VIII and factor VIII-like proteins. Preferred binding molecules of the present invention exhibit not only distinct characteristics for binding of the target factor VIII polypeptides but also specific and desirable characteristics for release (elution) of the target polypeptides. Especially preferred binding molecules according to the invention are short polypeptide sequences, characterized by a stable loop structure. | 11-18-2010 |
20100331522 | CAPTURE AND ELUTION OF BIO-ANALYTES VIA BEADS THAT ARE USED TO DISRUPT SPECIMENS - Lysing may include agitating a specimen in a chamber along with a medium that includes a particulate lysing material that has an affinity for a biological material. Lysing material may include beads or other material which may be coated that facilitates binding. The medium may include a fluid with a high salt or low pH level. The biological material may be eluted by lowering a concentration of salt or increasing a pH level. Lysing materials with two or more different affinities may be employed. Heating may be used. Lysing may be performed in a flow through apparatus. | 12-30-2010 |
20110015373 | IMPRINTED POLYMERS WITH AFFINITY FOR PHOSPHORYLATED PEPTIDES AND PROTEINS - The present invention relates to a method of separating or extracting phosphorylated amino acids, peptides or proteins with a molecularly imprinted polymer and to the preparation of said molecularly imprinted polymer as well as use of a molecularly imprinted polymer for separating or extracting phosphorylated amino acids, peptides or proteins. | 01-20-2011 |
20110065897 | METHOD FOR ISOLATING POLYPEPTIDES - The present invention relates to a method for isolating and/or purifying at least one polypeptide from a polypeptide-containing sample, characterized in that the sample is contacted with a boron carbide support material at a pH which allows the binding of the polypeptide to the boron carbide support material. Such isolating can, for example, be used to remove polypeptides from a sample or else to purify and/or to concentrate polypeptides. A matrix comprising a boron carbide support material for purification of polypeptides is further disclosed according to the invention. | 03-17-2011 |
20110071274 | IMMOBILISATION OF CHELATING GROUPS FOR IMMOBILISED METAL ION CHROMATOGRAPHY (IMAC) - The present invention refers to a method for binding a polycarboxylic acid to a solid phase. Further, the invention refers to a solid phase having a polycarboxylic acid immobilized thereto and methods of using the solid phase, e.g. for purifying His-tagged recombinant polypeptides. | 03-24-2011 |
20110098446 | Use of a tetraphenylborate (TPB) salt for the separation of biomolecules - Process for the separation of a biomolecule containing at least one cationic group from a liquid medium containing said biomolecule, which comprises the use of a tetraphenylborate (TPB) salt. | 04-28-2011 |
20110130542 | METHOD FOR THE SELECTIVE SEPARATION OF PEPTIDES AND PROTEINS BY MEANS OF A CRYSTALLIZATION PROCESS - Method for removing and selective separating peptides and proteins from a solution by controlled crystallization. | 06-02-2011 |
20110245461 | METHODS FOR DETERMINING THE RETENTION OF PEPTIDES IN REVERSE PHASE CHROMATOGRAPHY USING LINEAR SOLVENT STRENGTH THEORY - The present disclosure relates to methods for separating or isolating a peptide using reverse phase chromatography. The disclosure also relates to methods for calculating or determining the slope S of a peptide, wherein S is defined according to the Linear-Solvent-Strength equation log k=log k | 10-06-2011 |
20120041174 | Counter Current Purification of Polypeptides - The present invention relates to methods for the purification of polypeptides using counter current chromatography. In particular the present invention relates to the use of counter current chromatography for the purification of recombinant GLP-1. | 02-16-2012 |
20120123091 | MOVEABLE CHROMATOGRAPHY COLUMN SEPARATOR - The current invention is directed to a chromatography column separator which separates the chromatography column in an upper chromatography column chamber and a lower chromatography column chamber, and which has a variable position within the chromatography column, and which is embedded by the chromatography material. The separator allows the replacement of the chromatography material in the upper chromatography column chamber without the need to replace the chromatography column material in the lower chromatography column chamber and it allows also the combination of two different chromatography materials with different chromatographical functional groups in one chromatography column. | 05-17-2012 |
20120149872 | Channel-based purification device - The present invention relates to a device for purifying an analyte from a fluid sample. The device comprises a channel or tubing having an inner surface that binds to the analyte of interest in the fluid sample. As the fluid sample flows through the channel, the analyte of interest binds to the inner wall of the channel. The bound analyte is then eluted using a small bolus of elution buffer. The channel generates a high surface area for capturing the analyte in a large volume sample, but allows low liquid elution volume for concentrating the analyte into a small volume. | 06-14-2012 |
20120277407 | METHOD OF LIBERATING AND RECOVERING PEPTIDE - A novel method capable of liberating a peptide from a complex of peptide and albumin and the associated method of recovering the peptide are provided. When a liquid sample containing a complex of peptide and albumin undergoes heat treatment, a self-aggregate of albumin formed in the liquid sample. The peptide is simultaneously liberated from the complex and recovered by removing the self-aggregate from the liquid sample. | 11-01-2012 |
20120296068 | Peptide Chromatographic Purification Assisted by Combining of Solubility Parameter and Solution Conformation Energy Calculations - A method of purifying a compound from a mixture through a chromatographic column loaded with a column adsorbent. The method comprises:
| 11-22-2012 |
20120302731 | PROTEIN HYDROLYSATE, POLYPEPTIDE SOLUTION AND POLYPEPTIDE, PREPARATION METHOD AND USE THEREOF - The present invention provides methods for the preparation of protein hydrolysate, peptide solution and peptide from BSG. The wet BSG or BSG powder is dispersed in extract solution to prepare the crude BSG protein or the crude BSG protein solution. Preparing the crude BSG protein solution using the crude BSG protein and adjusting the pH to 6.5˜8.5, or adjusting the pH of the crude BSG protein solution to 6.5˜8.5. Then the solution is hydrolyzed with protease at 45° C. to 65° C. for 1 h to 5 h in a water bath shaker to prepare BSG protein hydrolysate. The protein hydrolysate is heated to inactivate the protease and centrifuged to obtain the peptide solution. The peptide solution is separated by gel filtration and each peak is collected and pooled together to obtain the peptide. The protein hydrolysate, peptide solution and peptide in the present invention are all prepared from BSG which is a natural product and available at low cost throughout the year. There is no harmful material used in the production process. The results of in vitro experiment suggest that BSG peptide prepared by this method shows a significantly hypoglycemic effect. | 11-29-2012 |
20120329989 | METHOD FOR PRODUCING A GRAPHITE-BASED PEPTIDE PURIFICATION MATERIAL AND METHOD FOR PEPTIDE PURIFICATION - The invention relates to a method for producing a graphite-based peptide purification material, which is characterized in that graphite is adjusted to a pH of <7 (acid) by incubation at least once in at least one organic or inorganic acid for at least one minute. The invention further relates to a method for peptide purification, wherein the peptide has a terminal planar aromatic protective group, using graphite in a packed form as the purification material, wherein the method is characterized in that previously acidified graphite (pH<7), which has been produced according to the method for producing a graphite-based peptide purification material, is used as the purification material. | 12-27-2012 |
20130035471 | Tetrameric Streptavidin Mutein With Reversible Biotin Binding Capability - The present invention relates to a new streptavidin muteins. This mutein is The muteins are capable of oligomerization to form tetramers, with relatively strong subunit interactions, dissociation constant (K | 02-07-2013 |
20130165632 | METHOD FOR SEPARATING WATER-SOLUBLE BIOLOGICAL SUBSTANCES - Provided is a novel method for separating water-soluble biological substances. A separating agent is composed by bonding a polysaccharide such as cellulose or amylose to the surface of a carrier by chemical bonding, and water-soluble biological substances are separated from a mixture of two or more types of water-soluble biological substances by chromatography using the separating agent. | 06-27-2013 |
20130281665 | PROCESS FOR PURIFYING CYCLOLIPOPEPTIDE COMPOUNDS OR THE SALTS THEREOF - A process for purifying cyclic lipopeptide compounds or salts thereof comprising the steps of: (1) charging a crude compound of Formula I onto a macroporous adsorption resin; (2) washing the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as a washing liquid; and (3) eluting the compound of Formula I from the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as an eluent. The purification method has the advantages of using a small amount of organic solvents, using no silica gel, and causing little damage to the environment; the purity of the collected compound of formula I is also improved as compared with the methods previously disclosed. | 10-24-2013 |
20140046023 | SPECIFIC SORBENT FOR BINDING PROTEINS AND PEPTIDES, AND SEPARATION METHOD USING THE SAME - Sorbent comprising a solid support material, the surface of which comprises first residues comprising a binuclear heteroaromatic structure comprising besides carbon atoms at least one of the heteroatoms N, O, S, and second residues comprising a mononuclear heteroaromatic structure comprising besides carbon atoms at least one of the heteroatoms N, O, S. | 02-13-2014 |
20140107319 | CARRIER FOR SEPARATION, METHOD FOR SEPARATION OF COMPOUND, AND METHOD FOR SYNTHESIS OF PEPTIDE USING THE CARRIER - Disclosed are a carrier for use for separation purpose and a method for separation of a compound which enable a chemical reaction to be performed in a liquid phase, enable a compound of interest to be separated from the liquid phase after the completion of the reaction readily, enable the separated compound to be evaluated by structural analysis or the like while the compound being bound to the carrier, and enable the compound to be separated from the carrier readily. A carrier for separation which has a reaction site capable of reacting with other compound on a benzene ring, and a long-chain group having a specified carbon atom(s) at each of the ortho-position and the para-position of the reaction site through an oxygen atom. | 04-17-2014 |
20140288269 | PROCESS FOR THE PREPARATION OF RANDOM POLYPEPTIDES AND EMPLOYING CIRCULAR DICHROISM AS A GUIDANCE TOOL FOR THE MANUFACTURE OF GLATIRAMER ACETATE - The present invention discloses novel process for the preparation of mixture of polypeptides comprising L-Glutamaic acid, L-Alanine, L-Tyrosine, and L-Lysine. by employing circular dichroism as a guidance tool. | 09-25-2014 |
20140296485 | ANIONIC DISPLACER MOLECULES FOR HYDROPHOBIC DISPLACEMENT CHROMATOGRAPHY - A process for separating organic compounds from a mixture by reverse-phase displacement chromatography, including providing a hydrophobic stationary phase; applying to the hydrophobic stationary phase a mixture comprising organic compounds to be separated; displacing the organic compounds from the hydrophobic stationary phase by applying thereto an aqueous composition comprising a non-surface active hydrophobic anionic displacer molecule and about 10 wt % or less of an organic solvent; and collecting a plurality of fractions eluted from the hydrophobic stationary phase containing the separated organic compounds; in which the non-surface active hydrophobic anionic displacer molecule comprises a hydrophobic anion and a counterion, CI, having the general formula A or B, as defined in the disclosure: [CM][Cl]d [CM-R*—CM′][Cl]d A B. | 10-02-2014 |
20140316108 | Protein Chromatography Matrices with Hydrophilic Copolymer Coatings - A coating of a random copolymer of acrylamide and a second monomer, e.g. glycidoxylmethacrylate, for a silica surface is described. The coating is applied to chromatographic support structures having silica based surfaces. The coating is functionalized to produce protein chromatography matrices that are particularly useful for extracting trace amounts of biomarker molecules from biological samples. | 10-23-2014 |
20140350219 | METHOD FOR SOLID PHASE SYNTHESIS OF LIRAGLUTIDE - Provided is a method for solid phase synthesis of liraglutide, comprising the following steps: A), Fmoc-Gly-resin being obtained by coupling resin solid phase carrier with glycine with N-end protected by Fmoc(Fmoc-Gly-OH) in the presence of activator system; B) according to the peptide sequence of the main chain of liraglutide, successively coupling with amino acids with N-ends protected by Fmoc and protected side chains by the method for solid phase synthesis, wherein lysine employs Fmoc-Lys(Alloc)-OH; C) removing the protective group, Alloc, from the side chain of lysine; D) couplilng the side chain of lysine with Palmitoyl-Glu-Offiu by the method for solid phase synthesis; E) cleavage, removing protection groups and resin to obtain crude liraglutide; F) purifying and lyophilizing to obtain liraglutide. | 11-27-2014 |
20190144494 | TREATMENT OF KERATIN-CONTAINING BIOLOGICAL MATERIALS | 05-16-2019 |