Entries |
Document | Title | Date |
20080199946 | Biochip - The present invention relates to a biochip for nucleic acid hybridization. The biochip of the present invention comprises a hybridization chamber which is in the form of a cavity, a porous matrix pressed in the hybridization chamber; and at least one first circulation hole and at least one second circulation hole which are communicated with the hybridization chamber so that the reaction solution flows in the at least one first circulation hole and flows out the at least one second circulation hole through the pores of the porous matrix. The hybridization reaction area is increased by flowing the reaction solution through the pores of the membrane, which enable the reaction sensitivity to be increased. The diffusion distance for the reaction molecules is decreased due to the limited inside space of the membrane, and thereby the hybridization time is shortened. | 08-21-2008 |
20080206848 | NUCLEIC ACID ANALYSIS DEVICE BY OPTICAL DETECTION USING DISK - There is provided a method or structure of carrying out optical measurement for the change of the state of the reactive sample solution at two or more measurement points of the channel simultaneously and continuously in a flow system reaction in which the reactive proceeds according to the elapsed time for movement. A sample containing nucleic acids is made to flow to a channel of which a temperature control means controlling the temperature of the passing area so that the temperature changes in the repetitive pattern, and the change in the state of the sample containing nucleic acids flowing on the channel is detected by optical detection means at two or more places of the channel. The channel can be placed in the analysis area on the disk driven by rotation, and information of the reactive sample solution in the channel can be detected simultaneously and continuously by optical detection means which is installed facing the disk for nucleic acid analysis. The constitution of the structure allows providing compact device for nucleic acid analysis is provided with a flow system reaction tube of the sample containing nucleic acids and an optical detection means. | 08-28-2008 |
20080206849 | Assay Device & Method - There is disclosed a lateral flow assay device and method of producing the same. The device, which is for identifying carbohydrate antigens in a biological sample comprises a substrate having, a) a sample receiving zone, b) an extraction zone for receiving the sample from said sample receiving zone and which extraction zone comprises immobilised or otherwise absorbed therein at least one or more reactants and reagents which when combined react to form an extraction reagent for a desired antigen in said sample, said sample to be added to said sample receiving zone optionally comprising the remaining reactant required to form said extraction reagent if not present in said extraction zone, c) optionally a neutralising agent capable of bringing the pH of the resulting sample to within the operational pH range of the assay and, d) a detection zone for a labelled specific binding or capture reagent for said antigen to be detected. | 08-28-2008 |
20080206850 | Methods and compositions for DNA synthesis - In some embodiments, the present disclosure relates to new phosphoramidite compositions, that have Silyl-containing carbonate or thiocarbonate or ether as 5′-hydroxyl protecting groups useful of the synthesis of DNA, and in particular for the synthesis of long sequences of DNA (e.g., >50 mer). In some embodiments, there are provided methods for simultaneous oxidation of the internucleoside phosphate triester linkages and removal of the 5′-hydroxyl-protecting group, making this process a new 2-step DNA synthesis, that involves the use of peroxyanions in combination with fluoride anions. | 08-28-2008 |
20080206851 | Methods and compositions for RNA synthesis - In some embodiments, the present disclosure relates to new phosphoramidite compositions, that have Silyl-containing carbonate or thiocarbonate or ether as 5′-hydroxyl protecting groups useful fo the syntheis of RNA, and in particular for the synthesis of long sequences of RNA (e.g., >50 mer). In some embodiments, there are provided methods for simultaneous oxidation of the internucleoside phosphate triester linkages and removal of the 5′-hydroxyl-protecting group, making this process a new 2-step RNA synthesis, that involves the use of peroxyanions in combination with fluoride anions. | 08-28-2008 |
20080206852 | SAMPLE PROCESSING APPARATUS - The present invention provides a sample processing apparatus and a sample container. In a sample processing apparatus, only after a locking member provided on a sample container engages with a sample container receiving portion, the locking member is released. Thereby, a sample fluid is transferred from the sample container to the sample processing apparatus so that various analysis processes can be performed. | 08-28-2008 |
20080206853 | SIGNAL ENHANCEMENT SYSTEM WITH MULTIPLE LABELED-MOIETIES - Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labeled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labeled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labeled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described. | 08-28-2008 |
20080206854 | Reactors (medical devices) in which chemical, biochemical, biological reactions - activations take place and their final products are used - Reactors (medical devices), which contains one or more independent of each other solutions and has two or more openings with corresponding indented hollow caps and/or pump and/or needle on the other opening as delivery system. The caps contain within them sensitive or incompatible enzymes, chemical, biological, or synthetic substances in powder or tablet form. Indicative of these caps is the fact that when impressed or twisted the protective seal within is broken, releasing their content into the solution or solutions contained within the vial in the order where the desired reactions will take place and the final products will be used by the user for pharmaceutical, cosmetic, nutritional, diagnostic, and other purposes. | 08-28-2008 |
20080220509 | Nucleic Acid Amplifier - Disclosed herein is a nucleic acid amplifier for carrying out a nucleic acid amplification reaction, the amplifier including at least: a plurality of wells configured to carry out the nucleic acid amplification reaction; a heating unit provided for every well; optical means capable of irradiating excitation light of a specified wavelength to all of the wells; and a fluorescence detection unit provided for every well. | 09-11-2008 |
20080220510 | DEVICE FOR DILUTING A FLUID AND DETECTING ANALYTES WITHIN A DILUTED FLUID - The present invention provides a device for processing, sampling, and diluting a fluid and for detecting an analyte within the processed, sampled, and diluted fluid. In the device for processing, sampling, and diluting a fluid, an amount of fluid to be processed, sampled, and diluted is accepted by a compressible porous membrane; a portion of the membrane saturated with the fluid is isolated thereby defining a predetermined sample volume of fluid; and the predetermined fluid volume sample is then released from the isolated membrane with a specified quantity of a fluid diluent to provide a diluted fluid sample. Analytes within the diluted fluid sample are detected by test strips in fluid contact with the diluted fluid sample. | 09-11-2008 |
20080227185 | Diagnostic System for Carrying Out a Nucleic Acid Sequence Amplification and Detection Process - An integrated lab-on-a-chip diagnostic system for carrying out a sample preparation process on a fluid sample containing cells and/or particles, the system comprising: (a) an inlet for a fluid sample; (b) a lysis unit for lysis of cells and/or particles contained in the fluid sample; (c) a nucleic acid extraction unit for extraction of nucleic acids from the cells and/or particles contained in the fluid sample; (d) a reservoir containing a lysis fluid; (e) a reservoir containing an eluent for removing nucleic acids collected in the nucleic acid extraction unit; wherein the sample inlet is in fluid communication with the lysis unit, an optional valve being present to control the flow of fluid therebetween; wherein the lysis unit is in fluid communication with the nucleic acid extraction unit, an optional valve being present to control the flow of fluid therebetween; wherein the reservoir containing the lysis fluid is in fluid communication with the lysis unit, an optional valve being present to control the flow of fluid therebetween; and wherein the reservoir containing the eluent is in fluid communication with the nucleic acid extraction unit, an optional valve being present to control the flow of fluid therebetween. | 09-18-2008 |
20080227186 | Active, micro-well thermal control subsystem - Devices and systems for active thermal control of sample holding devices for bDNA testing, polymerase chain reaction testing, chemiluminescent immuno-assay testing, and so forth. The thermal control subsystem includes a fluidic circuit, first and second heater assemblies, a centrifugal pump, and a heat exchange device. The first and second heater assemblies include a heat removal device and a controllable thermo-electric device. One or both of the heater assemblies can include a heat spreader. A controller actively controls the pump, the heat removal device, and the thermo-electric devices, to thermally-control sample-containing vessels retained in the holding device. | 09-18-2008 |
20080233634 | NUCLEIC ACID DETECTION DEVICE - A nucleic acid detection device is provided with a closed channel formed of a first channel portion, through which a washing reagent storage section for storing a washing reagent for washing a detection section for nucleic acid detection communicates with the detection section, and a second channel portion through which a pretreatment section for nucleic acid treatment communicates with the detection section. The closed channel is connected with a gas inlet/outlet path for communication with the outside. The gas inlet/outlet path is blocked by a sealing mechanism before nucleic acid detection. In storing a pretreatment reagent and the washing reagent frozen, the gas inlet/outlet path is kept open and connected to the channel. Thus, there is provided a nucleic acid detection device having a structure for preventing leakage of nucleic acid samples to the outside and which can be stored for a long period of time with the reagents therein. | 09-25-2008 |
20080248561 | Molecular characterization with carbon nanotube control - There is provided a first reservoir containing a liquid solution including a molecule to be characterized and a second reservoir for containing a liquid solution including a molecule that has been characterized. A solid state support structure is provided including an aperture having a molecular entrance providing a fluidic connection to the first reservoir and a molecular exit providing a fluidic connection to the second reservoir. One carbon nanotube is provided having a longitudinal sidewall disposed as a molecular contacting surface at the aperture. A voltage source is connected in series with the carbon nanotube for electrically biasing the carbon nanotube, and an electrical current monitor is connected in series with the carbon nanotube for monitoring changes in electrical current through the nanotube corresponding to translocation of a molecule through the aperture. | 10-09-2008 |
20080254530 | ISOLATION AND CHARACTERIZATION OF A SINGLE MITOCHONDRION - A system for identifying mitochondrial heteroplasmy within eukaryotic cells is provided. This system includes means for isolating and capturing a single mitochondrion from at least one eukaryotic cell, wherein the means for isolating and capturing a single mitochondrion further includes optical tweezers or a similar optical technology; means for analyzing the isolated and captured mitochondrion, wherein the means for analyzing the isolated and captured mitochondrion further includes a DNA amplification system and a sequencing system for amplifying and sequencing DNA extracted from the mitochondrion; means for identifying at least one mitochondrial heteroplasmy of interest; and means for using the DNA amplification and DNA sequencing systems to determine the presence or absence of the mitochondrial heteroplasmy within the eukaryotic cell from which the mitochondrion was obtained. | 10-16-2008 |
20080268528 | Automated analyzer for use in performing nucleic Acid-based amplification reactions - An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations, or modules, in which discrete aspects of the assay are performed on fluid samples contained in reaction receptacles. The analyzer includes stations for automatically preparing a specimen sample, incubating the sample at prescribed temperatures for prescribed periods, performing an analyte isolation procedure, and ascertaining the presence of a target analyte. An automated receptacle transporting system moves the reaction receptacles from one station to the next. The analyzer further includes devices for carrying a plurality of specimen tubes and disposable pipette tips in a machine-accessible manner, a device for agitating containers of target capture reagents comprising suspensions of solid support material and for presenting the containers for machine access thereto, and a device for holding containers of reagents in a temperature controlled environment and presenting the containers for machine access thereto. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte. The process is performed by automatically moving each of a plurality of reaction receptacles containing a solid support material and a fluid sample between stations for incubating the contents of the reaction receptacle and for separating the target analyte bound to the solid support from the fluid sample. An amplification reagent is added to the separated analyte after the analyte separation step and before a final incubation step. | 10-30-2008 |
20080280353 | Hybrid nucleic acid assembly - A detection device comprised of a hybrid nucleic acid assembly. The device further comprises a nucleic acid polymer, a first nanoparticle conjugated to the nucleic acid polymer, a second nanoparticle conjugated with the nucleic acid polymer, a means for introducing energy into the first nanoparticle, means for detecting energy from the second nanoparticle, and means for determining a physical property of the nucleic acid polymer, wherein the means of introducing energy into the first nanoparticle is a source of mechanical energy. | 11-13-2008 |
20080293128 | SENSOR OF PYROPHOSPHATE AND SNP TYPING SENSOR USING THE SAME - A sensor of pyrophosphate which can detect pyrophosphate conveniently with high sensitivity in a method for measuring pyrophosphate in SNP typing utilizing a primer extension reaction is provided. | 11-27-2008 |
20080293129 | Fluid Handling Unit and Fluid Handling Apparatus Using Same - A fluid handling unit | 11-27-2008 |
20080305537 | Microchips and its Manufacturing Methods Thereof - A microchip is provided with a lower substrate configured as the lower portion of the microchip, an intermediate section formed on the top of the lower substrate, and an upper substrate formed on the top of the intermediate section, wherein the lower substrate, the intermediate section, and the upper substrate are made of light-transmissive and cured resin. | 12-11-2008 |
20090004729 | REACTION DEVICE - A reaction device including: a plurality of reaction areas; and a plurality of heating parts configured to be each provided for a respective one of the reaction areas, wherein each of the heating parts includes: a heat source; a scan line for selecting the heating part; a data line that transmits heating amount information used for heating to the heat source; a writer that acquires the heating amount information transmitted from the data line; a holder that stores the heating amount information also after the scan line is turned to a non-selected state; and a heat generation controller that controls heat generation of the heat source based on the heating amount information. | 01-01-2009 |
20090017528 | Nucleic Acid Hybridization Device - The present invention relates to a hybridization device coupled by means of vibration to shorten the time needed for hybridization. This hybridization device of the present invention comprises an oscillation generating unit; a spacer attached to the top of the oscillation generating unit, which wraps around to form a tank, and the tank is loaded with a transmission solution; a loading plate having a loading face was paved with at least one nucleic acid probe, and the flip side of the loading face is a transmission face, which covers on top of the spacer to form a closed space, and is attached with the transmission solution. The vibration generated by the oscillation generating unit can be propagated to the loading plate through the transmission solution to promote the effective mixing of the nucleic acid to be analyzed and the probe, further to shorten the time needed for nucleic acid hybridization and to increase the detection sensitivity. | 01-15-2009 |
20090023201 | NUCLEIC ACID DETECTING CASSETTE AND NUCLEIC ACID DETECTING APPARATUS - In a nucleic acid detecting cassette in which a heating treatment chamber and a liquid delivery channel are constituted of a stationary member and flexible members, the heating treatment chamber is held by convex heating sections from both sides, whereby the heat treatment chamber and the liquid delivery channel are spatially separated from each other. Thus, it is possible to automatically execute a consistent process including nucleic acid amplification and other required treatments of the object sample as well as detection of a target nucleic acid. | 01-22-2009 |
20090042280 | FLUIDIC CARTRIDGES FOR ELECTROCHEMICAL DETECTION OF DNA - A flow cell cartridge for the detection of differences in nucleic acid sequences is disclosed. The flow cell cartridge has an electrode array and two openings, in which one opening is for the entry and exit of sample, and the other opening is for the control of the entry and exit of sample through the exertion of negative and positive pressure. The entire flow cell cartridge can be moved from sample to sample to allow different samples to be drawn into the cartridge into contact with an electrochemical electrode array, thus allowing reactions to occur in the chamber itself. | 02-12-2009 |
20090042281 | COMPOUND PROFILING DEVICES, SYSTEMS, AND RELATED METHODS - High throughput compound profiling systems, and related devices and sub-systems that can be used to perform various compound profiling processes are provided. These systems typically include work perimeters that are organized for optimum efficiency and processing accuracy. Further, these systems are readily adaptable for performing a wide array of assays, as many different system components are easily incorporated or interchangeable in a particular system. System components that are provided by the invention include cell culture dissociators, which can be used, e.g., to effect cell wetting, dissociation, and/or agitation applications. In some embodiments, these cell culture dissociators are included as components of automated cell culture passaging stations. Dispensing devices that permit on-the-fly fluid temperature regulation are also provided. In addition, various compound profiling methods, cell dissociation methods, uniform cell concentration dispensing methods, among other processes, are also provided. | 02-12-2009 |
20090053799 | TRAPPING MAGNETIC SORTING SYSTEM FOR TARGET SPECIES - A system for sorting and trapping magnetic target species includes a microfluidic trapping module designed to receive and then temporarily hold magnetic particles in place within the module. The magnetic particles flowing into the module are trapped there while the other sample components (non-magnetic) continuously flow through and out of the station, thereby separating and concentrating the species captured on the magnetic particles. The magnetic particles and/or their payloads may be released and separately collected at an outlet after the sample passes through the trapping module. | 02-26-2009 |
20090061505 | APPARATUS FOR SELECTIVE EXCITATION OF MICROPARTICLES - Nucleic acid microparticles are sequenced by performing a sequencing reaction on the microparticles using one or more selectively exciting the microparticles in an excitation pattern, optically imaging the microparticles at a resolution insufficient to resolve individual microparticles, and processing the optical images of the microparticles using information on the excitation pattern to determine the presence or absence of the optical signature, which indicates the sequence information of the nucleic acid. An apparatus for optical excitation of the microparticles comprises an optical fiber delivering a first laser beam, and an interference pattern generation module coupled to the optical fiber. The interference pattern generation module splits the first laser beam into second and third laser beams and generates the excitation pattern for selectively exciting the microparticles by interference between the second and third laser beams. | 03-05-2009 |
20090087901 | Analytical Test Element and Method for Blood Analyses - An analytical test element for blood analyses is provided having an application site and a microfluidic channel structure in fluid communication with the application site. The channel structure comprises at least first and second analytical channels for receiving first and second portions of a blood sample applied to the application site. The first analytical channel comprises a first analytical site to determine the total haemoglobin value (Hb) of the blood sample. The second analytical channel comprises a second analytical site to determine a glycohaemoglobin value (HbA1c) of the blood sample. | 04-02-2009 |
20090111170 | Nucleic acid processing methods, kits and devices - Biological samples containing nucleic acids, RNA and DNA, are freed from bound proteins by incubation with a chaotropic agent such as a guanidium salt, and the mixture is readied for further processing by dilution of such agent to a concentration below 0.05 M without physical isolation of RNA and DNA from one another or from other components of the reaction mixture. Methods include such preparation and further processing, such as amplification and detection, which may be performed in a single container. Chaotropic agent may be supplied as a dried reagent adhered to a container. Kits may include such reagents, alone or with amplification reagents. | 04-30-2009 |
20090117645 | Biochips and methods of fabricating the same - Biochips for analyzing components of a biological sample using probes and methods of fabricating the same are provided. In some embodiments, a biochip includes a substrate, a plurality of probes immobilized on a top surface of the substrate, and a capping layer formed on a bottom surface of the substrate. | 05-07-2009 |
20090117646 | DEVICE, KIT AND METHOD FOR PULSING BIOLOGICAL SAMPLES WITH AN AGENT AND STABILISING THE SAMPLE SO PULSED - A device and kit for pulsing a biological sample with a pulsing agent is disclosed. The biological sample so pulsed is subsequently stabilized and the device or kit provides a control reaction. Applications for the described device and kit are found in the field of medical diagnostics, particularly immunology. | 05-07-2009 |
20090123994 | Lockable Signalling Column - The invention relates to a signalling column with levels which are assembled by means of a relative pivoting limited in amplitude and interconnected by means of axially-extending conductors. The body of a level (A) has a locking bar ( | 05-14-2009 |
20090130745 | Integrated Apparatus for Performing Nucleic Acid Extraction and Diagnostic Testing on Multiple Biological Samples - The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides. | 05-21-2009 |
20090130746 | MICROCHANNEL SURFACE COATING - The present invention relates to a method for improving the efficiency of biochemical reactions in channels of microfluidic devices. More specifically, the present invention relates to the use of chitosan or a chitosan derivative for coating channel surfaces to reduce non-specific adsorption of reagents to microfluidic channels. This reduction of non-specific adsorption improves the efficiency and reproducibility of the reaction, e.g., amplification reactions, such as PCR, and reduces cross-contamination. | 05-21-2009 |
20090137029 | Multi-Chambered Receptacles - A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle. | 05-28-2009 |
20090148932 | USING COULOMB FORCES TO STUDY CHARATERISTICS OF FLUIDS AND BIOLOGICAL SAMPLES - A LoC (Lab on a Chip) is described to analyze surface properties of fluid drops. Substrates with cavities near the edge are filled with fluids that have a contact angle greater than 90°. The surfaces of two different drops can be brought in contact with one another by using Coulomb forces. Several experiments can be carried out while the drops are in contact: the concentration of the fluid in each drop can be altered, tangential and normal forces can be applied to the contact surface, voltage differences across and current flow through the contact surface can be monitored. MEMS pumps can be used to mix reagents or buffers with the fluid to determine the protein concentration or to extract DNA from whole cells, respectively. Substrates holding optical components can be used to align fibers with either lasers or receivers. The alignment is automatic and controlled by a control unit. | 06-11-2009 |
20090148933 | INTEGRATED NUCLEIC ACID ASSAYS - Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed. | 06-11-2009 |
20090155891 | APPARATUS FOR DETECTING NUCLEIC ACID AMPLIFICATION PRODUCT IN REAL TIME - There is provided an apparatus for detecting a nucleic acid amplification product in real time, which is capable of effectively excluding or reducing apparatus error factors without using a second fluorescence signal used for correction. A plurality of wells | 06-18-2009 |
20090176298 | Polymer Compound For Medical Material, And Biochip Substrate Using The Polymer Compound - A main object of the invention is to provide a polymer compound for medicine which has an excellent capability of fixing a biologically active substance and has such chemical/physical stability that the compound is less dissolved or deteriorated in a washing step, in particular, which can be suitably applied to a plastic substrate surface. | 07-09-2009 |
20090176299 | Species Specific DNA Detection - The present invention relates to methods and compositions for the identification of species-specific material in pharmaceutical products. In particular, the present invention relates to methods for the identification of species-specific DNA in a population of a different species or following contact (e.g., growth) with cells of a different species. | 07-09-2009 |
20090186402 | Detection instrument with the use of polynucleotides mapped on barley chromosome - As the results of mass sequencing of cDNA clones originating in barley varieties “ | 07-23-2009 |
20090186403 | Biochip cartridge - The present invention provides a biochip cartridge wherein an elastic body is used for a substrate member in order to stabilize the feeding of blood or solution and whereby it is possible to avoid the risk of accidental contact of the operator with solutions due to mishandling. | 07-23-2009 |
20090191616 | Biosensor structure and fabricating method thereof - A biosensor structure and a method for fabricating the same are described. The biosensor structure for detecting at least a single cell includes a substrate with an insulating surface, a conductive layer and a plurality of capture molecules. The conductive layer is disposed on the substrate, and has a first pattern and a second pattern separated from each other. The first pattern includes a plurality of first finger configurations, and the second pattern includes a plurality of second finger configurations, so as to form interdigitated array. The capture molecules are immobilized on the conductive layer, such that the cell that is bound specifically to the capture molecules on two adjacent first and second finger configurations is detected. The biosensor structure is feasible for real-time (<3 min), specific, and quantitative targeted cell detection down to a single cell. | 07-30-2009 |
20090197326 | Biological Analysis Arrangement and Approach Therefor - Characteristics of a chemical or biological sample are detected using an approach involving light detection. According to an example embodiment of the present invention, an assaying arrangement including a light detector is adapted to detect light from a sample, such as a biological material. A signal corresponding to the detected light is used to characterize the sample, for example, by detecting a light-related property thereof. In one implementation, the assaying arrangement includes integrated circuitry having a light detector and a programmable processor, with the light detector generating a signal corresponding to the light and sending the signal to the processor. The processor provides an output corresponding to the signal and indicative of a characteristic of the sample. | 08-06-2009 |
20090209028 | Sensing Chip - There is provided a sensing chip capable of measuring a refractive index by utilizing a long-range surface plasmon polariton, accurately measuring an accumulative refractive index in a wide range, and more easily enabling sealing for measurement. The present invention relates to the sensing chip which has a thin metal film or a strip-like metal grown on an underlayer, and has a dielectric that limits a refractive index and a dielectric buffer layer on an upper surface and a lower surface of the thin metal film or the strip-like metal. The dielectric buffer layer is attached onto the thin metal film or the strip-like metal. The thin metal film or the strip-like metal and the buffer layer are sandwiched between two dielectric layers. A hole is made in a surface of the upper dielectric layer to serve as a measurement groove. | 08-20-2009 |
20090215157 | METHODS FOR PATHOGEN DETECTION - The present disclosure relates to methods, systems, devices, and microfluidic chips that may be used for the detection of pathogens. | 08-27-2009 |
20090215158 | Micro Flow Channel Chip - A micro flow channel chip of the present invention has multiple grooves formed on a substrate to be connected with each other in parallel or in series and capillaries, chemically-modified in a manner different from each other, laid in the multiple grooves, wherein detected data can be obtained by supplying a fluid to the capillaries laid in the multiple grooves. The micro flow channel chip enables multiple items of chemical and biochemical functions to be measured simultaneously. The micro flow channel chip enables multiple items of chemical and biochemical functions to be measured simultaneously using micro flow channels formed on the substrate. | 08-27-2009 |
20090215159 | DEVICE FOR HANDLING AND ANALYSIS OF A BIOLOGICAL SAMPLE - An apparatus for detection of an analyte of interest in a solid, semi-solid, or liquid biological sample, using a lateral flow assay test strip, is described. The apparatus minimizes sample handling and manipulation upon introduction of the sample into a sample-receiving chamber and transfer of the sample to a test chamber containing the assay strip. | 08-27-2009 |
20090221059 | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples - The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides. | 09-03-2009 |
20090221060 | NUCLEIC ACID ANALYZER - It is an object of the present invention to prevent condensation occurring when reaction solutions in sealed vessels are heated in a nucleic acid analyzer for performing sequential processing in which individual reaction vessels are sequentially fed to a nucleic acid amplification unit in a given cycle. This invention relates to a nucleic acid analyzer including a vessel mounting rack capable of holding the plurality of reaction vessels, wherein the reaction vessel mounted on the vessel mounting rack is heated by an adjacent noncontact heat source and air circulation from the heat source to a reaction vessel upper portion, and a temperature of the reaction vessel upper portion is kept higher than a temperature of a reaction vessel lower portion. With this invention, condensation possibly occurring on the inner wall of the sealed vessel upper portion due to heating during nucleic acid amplification can be prevented, and more accurate nucleic acid analysis is enabled. | 09-03-2009 |
20090239290 | SINGLE-TUBE, READY-TO-USE ASSAY KITS, AND METHODS USING SAME - An assay kit is provided that includes assay reagents stored in a single-tube container, and a data storage medium containing information about the contents of the container. Methods are provided for using the data provided with the kit to direct instruments and/or processes, for example, to control an instrument to perform amplification and/or sequencing reactions. | 09-24-2009 |
20090253194 | DNA ANALYSIS APPARATUS - Accurate and sensitive sequencing in pyrosequencing is achieved by allowing complementary strand synthesis reaction to proceed homogeneously and completely in a short time while performing luminescence reaction for a sufficiently long time. DNA as a sequencing target is immobilized on the surface of a solid supporter. Nucleic acid substrates are injected from a dispenser to the supporter site where complementary strand synthesis is in turn performed rapidly and completely in a short time under a small reaction volume. Next, the supporter together with the product thereon is moved into a luminescence reaction solution where luminescence reaction is in turn performed. Thus, a DNA complementary strand synthesis reaction site and a luminescence reaction site are completely separated. The-supporter surface is also washed by dipping the supporter in the luminescence reaction solution that contains a luminescence reagent and an enzyme that degrades redundant nucleic acid substrates. | 10-08-2009 |
20090258414 | SYSTEM FOR FLUORESCENCE MONITORING - The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye. | 10-15-2009 |
20090258415 | Fluid Manipulation Assembly - Microfluidic assemblies, systems, and methods are provided for manipulating fluid samples. Assemblies include an elastically deformable cover layer and a less elastically deformable substrate. The methods include deforming the substrate through the cover layer so that when the cover layer rebounds a new communication results in the assembly between the cover layer and the substrate and/or so that a new barrier wall is formed. Systems for carrying out the methods are also provided. | 10-15-2009 |
20090263891 | DEVICE FOR ANALYZING SAMPLES INCLUDING A GAS EVOLVING MEANS - The invention relates to a device for analyzing one or more samples for the presence, amount or identity of one or more target molecules in the samples, comprising one or more capture sites whereby the device comprises a gas evolving means. The gas evolved by the gas evolving means moves unbound target molecules away from the capture site and therefore helps to increase the efficacy of the analysis. | 10-22-2009 |
20090275116 | Metallic PCR frames - The present invention is represented by a two part PCR well plate. Part one, the outer shell, is constructed from a thin metal.. Part two, the tubes, are thermoformed in an array of 96/384 or formed individually and inserted or welded into each tube cell location. The use of a rigid metal frame for the PCR array virtually eliminates all warping problems associated with temperature cycling. | 11-05-2009 |
20090275117 | SLIP COVER FOR HEATED PLATEN ASSEMBLY - A heated platen assembly for use in a biological testing device is disclosed having a heated platen defining a plurality of optical openings configured to permit radiation to pass through the heated platen, a light transmissive slip cover configured to cover at least one of the plurality of optical openings, and means for retaining the slip cover over the at least one of the plurality of optical openings. | 11-05-2009 |
20090275118 | Bacteria Detecting Instrument, Bacteria Detecting Method, and Bacteria Detecting Kit - A micro array instrument is used in which an oligonucleotide based on a species- or genus-specific nucleotide sequence of subject bacteria is immobilized on a surface of a substrate. By confirming whether the oligonucleotide immobilized on the substrate has hybridized with a probe prepared from a test sample, bacteria contained in the test sample can be detected and identified easily, quickly, and accurately. | 11-05-2009 |
20090280559 | GENETIC SEQUENCER INCORPORATING FLUORESCENCE MICROSCOPY - A fluorescence microscopy sequencer comprises a fluid transport subsystem in which reagents are pumped through a series of multi-port valves to a mixer or one or more flow cells, or directly into the flow cell(s). The one or more multi-port valves can be mounted upon a fluids manifold having syringe tubes mounted on the opposite side. Mounted on a movable support, the manifold may be brought into and out of fluid communication with a storage block comprising the plurality of reagents. In another embodiment, the sequencer comprises a beamsplitter indexer that facilitates the quick and reliable switching of filter cubes through use of a stepper motor. In yet another embodiment, a motion control system is provided in which an inertial reference is interposed between and directly coupled to a first and second axis of control, thereby minimizing any low structural resonant frequencies and enabling high performance (high frequency response) motion control. | 11-12-2009 |
20090291487 | Thermocycling Device - The invention relates to a thermocycler module for heating and/or cooling of a thermocycling device comprising a thermal switch, a heating block and a heat sink. The thermal switch comprises a thermoconducting liquid, e.g. a magnetic fluid or a liquid metal, and a stimulating unit, e.g. a magnetic unit or a Lorentz-force unit, for moving the thermoconducting liquid. The movement of the thermoconducting liquid provides, in an on-state of the thermal switch, a thermal connection between the heating block and the heat sink. | 11-26-2009 |
20090298160 | Reaction vessel and reaction controller - The invention relates to a reaction vessel and a reaction controller, wherein temperature control of a liquid stored within the vessel can be performed with a high accuracy and faithful responsiveness. The reaction container and the reaction controller comprise one or a plurality of reaction chambers in which liquid is storable, and a wall that surrounds the reaction chamber, and the entirety or a part of the wall is formed by a temperature raising and lowering body that can raise or lower the temperature thereof according to a signal from an instructing part provided outside. | 12-03-2009 |
20090298161 | Surface Treatment - A biosensor comprising surface treated with a method for producing a monolayer of molecules on a surface, the method comprising loading a stamp with seed molecules, transferring seed molecules from the stamp to the surface, wherein transferring comprises transferring a fraction of the seed molecules loaded on the stamp to the surface and adsorbing the seed molecules to the stamp and adsorbing the seed molecules to the surface, the adsorption of the seed molecules to the stamp being stronger than the adsorption of the seed molecules to the surface, self-completing amplification of the seed molecules via an amplifying reaction to produce the monolayer on a flat surface, wherein self-completing amplification comprises producing a homogeneous area, wherein the homogeneous area comprises a monolayer of molecules on the surface, and wherein the monolayer of molecules on the surface has no diffusive component that can relocate and destroy amplification accuracy. | 12-03-2009 |
20090305394 | DNA CHIP FOR DIAGNOSIS OF CORNEAL DYSTROPHY - The present invention relates to oligonucleotides for diagnosis of corneal dystrophy. More particularly, the present invention relates to oligonucleotides for detecting mutation of BIGH3 gene for diagnosis or corneal dystrophy including Avellino corneal dystrophy, which must be precisely diagnosed before vision correction surgery, and a DNA chip for diagnosis of corneal dystrophy, which has the oligonucleotides fixed thereon. According to the present invention, conventional microscopic diagnosis of corneal dystrophy can be replaced with a precise genetic method, which prevents a patient with corneal dystrophy from losing eyesight by eyesight correction surgery after erroneous diagnosis. | 12-10-2009 |
20090317897 | REACTION VESSEL, REACTION MEASURING DEVICE, AND LIQUID ROTATING TREATMENT DEVICE - An object is to provide a reaction vessel, a reaction measuring device, and a liquid rotating treatment device wherein temperature control of a liquid stored within the vessel can be performed with a high accuracy and faithful responsiveness. The construction is such that a vessel has: a storage chamber in which a liquid is storable, that has an opening part, and a reaction chamber that is communicated with the storage chamber and is formed thinner or narrower than the storage chamber, and the vessel is installable on a rotatable rotating body provided externally, and is formed such that when the vessel is installed on the rotating body, a rotation axis of the rotating body passes through the vessel, and the reaction chamber is positioned farther away from the rotation axis than the storage chamber. | 12-24-2009 |
20090317898 | Apparatus for Determining Gene Polymorphism - In a preferred embodiment, a gene polymorphism diagnosing reaction vessel at least having a plurality of probe arrangement parts each individually holding a probe that emits fluorescence in accordance with each of a plurality of polymorphic sites is used. A controller ( | 12-24-2009 |
20090317899 | Handling Kit for Analyzing a Liquid Sample by Nucleic Acid Amplification - The invention refers to a handling kit for analyzing a liquid sample, especially by nucleic acid amplification, comprising a disposable sample holding and processing device ( | 12-24-2009 |
20090325276 | INTEGRATED MICROFLUIDIC ASSAY DEVICES AND METHODS - Combinations of microfluidic diagnostic testing modules for simultaneous evaluations of serological and molecular biological targets are provided, and include panel testing for both antibodies (or antigens) and nucleic acid targets in one single-use device. These improvements are directed to evaluating the overall progress and activity of a pathogenic process in real time, at the point of care, not merely the presence or absence of a particular diagnostic marker, which can often be incomplete or misleading. | 12-31-2009 |
20090325277 | Thermal Device, System, and Method, for Fluid Processing Device - A device, system, and method are provided for thermally treating a fluid processing device. According to various embodiments, a system is provided that can include a thermal device and a fluid processing device holder. The thermal device can include a first block having a thermal conductivity greater than 0.5 Watt per centimeter Kelvin (W/cm·K), a second block having a thermal conductivity greater than 0.5 W/cm·K, and a heat-pump device disposed between the first block and the second block. The heat-pump device can transfer thermal energy from at least one of the first block and the second block to the other of the first block and the second block. The fluid processing device holder can hold a fluid processing device in a heat-transfer position with respect to the first block and the second block. The fluid processing device can be a microfluidic device. | 12-31-2009 |
20090325278 | Reaction System for Performing in the Amplification of Nucleic Acids - A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions. | 12-31-2009 |
20100009432 | CONDUCTIVE CARBON NANOTUBES DOTTED WITH METAL AND METHOD FOR FABRICATING A BIOSENSOR USING THE SAME - Conductive carbon nanotubes (CNTs) obtained by dotting carboxylated CNTs with metal nanocrystals by chemical functional groups, are described, as well as a method for fabricating a pattern or film of the conductive CNTs which involves repeatedly depositing conductive CNTs on a substrate to achieve high surface density. A biosensor is described, in which bioreceptors that bind to target biomolecules are selectively attached to conductive CNTs or a conductive CNT pattern or film. By use of the conductive biosensor, various target biomaterials that bind or react with the bioreceptors can be precisely measured directly or by electrochemical signals at large amounts in one step. Additionally, the biosensor can be used for an electrical detection method capable of providing precise measurement results even with a small amount of source material. | 01-14-2010 |
20100028983 | MICROWAVE ACCELERATED ASSAYS - The present invention provides for increasing fluorescence detection in surface assay systems while increasing kinetics of a bioreaction therein by providing low-power microwaves to irradiate metallic materials within the system in an amount sufficient to increase heat thereby affecting the kinetics of a bioreaction therein. | 02-04-2010 |
20100028984 | DEVICES AND METHODS FOR BIOCHIP MULTIPLEXING - The invention is directed to a method of analyzing a plurality of biochips. In particular, the method includes inserting a first biochip in to a first station of an analysis device, inserting a second biochip into a second station of the analysis device, wherein each of the first and second biochips comprise a substrate that includes an array of detection electrodes, each including a different capture binding ligand, a different target analyte, and a label, and a plurality of electrical contacts, detecting current as an indication of the presence of the labels on the first biochip and detecting current as an indication of the presence of the labels on the second biochip. | 02-04-2010 |
20100028985 | Reaction Vessel, Reaction Vessel Processing Apparatus and Diagnostic Apparatus - A reaction vessel suitable for automating of various measurements is provided. In a preferred embodiment, on the same side of plate-like substrate ( | 02-04-2010 |
20100028986 | REACTION CONTAINER PLATE AND REACTION TREATMENT APPARATUS - Disclosed herein is a reaction container plate which prevents the entry of foreign matter from the outside and the pollution of an outside environment. A reaction container plate ( | 02-04-2010 |
20100035336 | Cartridge Retaining Mechanism for Nucleic Acid Extracting Apparatus - A cartridge retaining mechanism equipped in a nucleic acid extracting apparatus for extracting a nucleic acid, the cartridge retaining mechanism comprising: a cartridge having a cylindrical shape with a bottom, and the bottom is shaped in a funnel shape; a nucleic acid-adsorbing solid carrier that traps a nucleic acid, and the nucleic acid-adsorbing solid carrier is disposed at the bottom of the cartridge; and a cartridge retaining member that retains the cartridge, wherein the cartridge retaining member comprises: a supporting part that supports the cartridge; and a pressure-proof retaining part that is attached to an open end of the cartridge, and wherein the pressure-proof retaining part has a nozzle receiving opening, onto which a pressure nozzle of the nucleic acid extracting apparatus is pressed, and wherein the cartridge is retained between the supporting part and the pressure-proof retaining part upon pressing the pressure nozzle onto the nozzle receiving opening. | 02-11-2010 |
20100041126 | METHODS FOR DIAGNOSING AND TREATING PROSTATE CANCER - Devices and methods for diagnosing and treating prostate cancer are disclosed. In practicing the method, a subject's body-fluid sample is assayed for GPC3 protein, and the GPC3 level observed is used in determining whether the subject has an elevated GPC3 level associated with prostate cancer. Patients with such elevated levels may be treated, in accordance with the present disclosure, with a variety of GPC3-related immunotherapy agents. | 02-18-2010 |
20100041127 | PREPARATION AND USE OF A REACTIVE SOLID SUPPORT SURFACE - A method of preparing a protein-resistant reactive solid support surface is disclosed. The method comprises the steps of providing a solid support having a hydrogel coating with a plurality of binding elements, coupling a protein resistant compound to the hydrogel via a first fraction of the binding elements, and coupling at least one binding agent to the hydrogel via a second fraction of the binding elements, whereby the protein resistant compound and the at least one binding agent are co-immobilized to the hydrogel. Also the use of the reactive surface in analysis, such as immunogenicity assays, is disclosed. | 02-18-2010 |
20100047901 | SYSTEM AND METHOD FOR ELECTRONIC SENSING OF BIOMOLECULES - A nanoelectronic device is combined with a cellular membrane component to provide a sensor for biomolecules or to provide information about the structure of the membrane. The nanoelectronic device may comprise a network of randomly-oriented nanotubes, or other nanostructure, arranged on a substrate with adjacent electrodes so as to operate as a field-effect transistor sensor or as a capacitive sensor. A cellular membrane is disposed over the nanostructure element. | 02-25-2010 |
20100055769 | BIOTECHNOLOGICAL DEVICE INCLUDING A STRUCTURED HYDROGEL PERMEATION LAYER - The present invention relates to a biotechnological device including a responsive hydrogel permeation layer with an intrinsic structural gradient. | 03-04-2010 |
20100055770 | HIGH-DENSITY MULTIWELL-PLATE - A high-density multiwell-plate for performing thermocycled amplification reactions of polynucleotides in liquid samples comprising a plurality of reaction wells is disclosed. In order to provide a better thermal insulation, the plate comprises a rigid well-forming structure placed above a bottom layer, wherein substantially horizontal well-covering areas cover the liquid sample comprised in the wells, and a substantially plane cover placed above the well-forming structure providing a thermal insulating air distance between the well-covering areas and the cover. | 03-04-2010 |
20100055771 | MICRO VALVE APPARATUS USING MICRO BEAD AND METHOD FOR CONTROLLING THE SAME - A micro (thin film type) valve apparatus for controlling fluid flow and its rate using microbead and a method for controlling the apparatus are provided. The microbead is moved by the magnetic forge generated by upper and lower electromagnets disposed on the top and bottom surface of the body or by the electric field generated by upper and lower electrode plates disposed on the top and bottom surface of the body, thereby interconnecting or blocking flow channels in the body. The micro valve apparatus and the method for controlling the same are suitable for thin film type diagnostic assay devices, such as lab-on-chips, protein chips, or DNA chips, for detecting small quantities of analytes in fluids, and more suitable for interconnecting or blocking channels formed in thin disc type apparatus, including general CD-ROMs, DVDs, bioCDs, and bio DVDs. | 03-04-2010 |
20100068797 | Method for the visual detection of specific antibodies by the use of lateral flow assays - This disclosure teaches a method and composition for detecting the presence of class specific antibodies reactive with analytes such as bacteria, allergens, autoimmune antigens, viral proteins, haptens, and carbohydrates by lateral flow techniques. In one embodiment of the disclosure, a test sample is added to the test strip, which then migrates to the site of the immobilized allergens, thereby forming a first antibody IgE-allergen complex. A chase release buffer is added upstream to or from the site of the labeled anti-IgE antibodies, which is itself upstream from the sample site. The anti-IgE antibodies migrate downstream to the site of immobilized first complex, thereby forming a second complex indicating the presence of class specific IgE antibodies in the test specimen. In another embodiment of the disclosure, a liquid form of the labeled anti-IgE antibodies can be added to the test strip after the first complex has been formed. | 03-18-2010 |
20100068798 | Intelligent multifunctional medical device apparatus and components - The multifunctional medical device contains system on chip (SoC) computation functionality to organize several functional modules consisting of analytical, diagnostic and therapeutic tasks in the microfluidic assembly. Micro-valves, micro-tubes, micro-wires and gates organize the chambers of the flexible modules which hold multiple chemical and biological agents for combination on demand. | 03-18-2010 |
20100068799 | ORGANIC SUBSTANCE DETECTING DEVICE AND ITS MANUFACTURE METHOD - A plurality of gold fine particles are discretely dispersed on and fixed to a principal surface of a substrate. Each of molecular probes is bonded to the gold fine particle at one end of the molecular probe. The molecular probe fixes a target capture portion at a tip thereof. The target capture portion has behavior of being specifically bonded to an organic molecule. | 03-18-2010 |
20100075407 | ULTRA-SENSITIVE DETECTION OF MOLECULES ON SINGLE MOLECULE ARRAYS - The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles to form a plurality of complexes, releasing at least a portion of some of the plurality of complexes, determining at least a portion of the plurality of complexes released, and determining a measure of the concentration of the analyte molecules or particles in a fluid sample. | 03-25-2010 |
20100086993 | CELL DETECTING SYSTEM AND QUANTUM DOT MEASURING SYSTEM - A cell detecting system is herein disclosed, wherein a complex is formed with a first bioactive ligand coupling to a quantum dot and recognizing and coupling to a first receptor of a cell and a second bioactive ligand coupling to a magnetic bead and recognizing and coupling to a second receptor of the cell. The magnet is configured for attracting the complex. A quantum dot measuring system configured for measuring the fluorescence of the complex includes an excitation light source, an optical system, a detecting sensor and a data capturing unit, wherein the detecting sensor includes a photomultiplier tube measuring florescence of the quantum dot excited by the excitation light source. The present invention achieves the goal of specific cell detection with high sensitivity without performing cell incubation. A quantum dot measuring system is also herein disclosed. | 04-08-2010 |
20100093068 | SYSTEM AND METHODS FOR NUCLEIC ACID SEQUENCING OF SINGLE MOLECULES BY POLYMERASE SYNTHESIS - This invention relates to improved methods for sequencing and genotyping nucleic acid in a single molecule configuration. The method involves single molecule detection of fluorescent labeled PPi moieties released from NTPs as a polymerase extension product is created. | 04-15-2010 |
20100093069 | Reagent Delivery System - A delivery apparatus for selectively delivering one or more liquid reagents into a reaction or test chamber ( | 04-15-2010 |
20100099175 | Two-Stage nucleic acid amplification using an amplification oligomer - This invention provides methods, compositions and systems to detect a nucleic acid of interest in a two-stage amplification. The two-stage amplification begins with a first non-enzymatic accumulation of an amplification oligomer that is the target substrate for a second nucleic acid amplification or assay. Two or more amplification oligomers can be used to allow multiplexed amplifications of two or more nucleic acids of interest with deconvolution based on unique detection signals or unique signal locations. | 04-22-2010 |
20100099176 | Systems for genome selection - Systems, methods, compositions and apparatus relating to genome selection are disclosed. | 04-22-2010 |
20100120132 | Bioassays by direct optical detection of nanoparticles - Embodiments of the invention relate to detecting biological molecules with ultra-sensitivity and convenience. The embodiments are especially directed to utilizing nanoparticles as tags and identifying the tags using dark-field microscopy. The probes containing the nanoparticles can be used in solution or attached to a substrate. | 05-13-2010 |
20100129898 | REAGENT DELIVERY SYSTEM - A delivery apparatus for selectively delivering one or more liquid reagents into a reaction or test chamber ( | 05-27-2010 |
20100136669 | MICROCHIP WITH ACCESSIBLE FRONT SIDE - The invention relates to a microchip assembly ( | 06-03-2010 |
20100144021 | Bio-driver apparatus for detecting a nucleic acid - A cleavable signal element applicable to quantitative and qualitative assay devices, using a cleavable technique specifically responsive to a complementary double strand or single strand of nucleic acids, and a nucleic acid hybridization assay method and device using the cleavable signal element are provided. Using the cleavable technique responsive to the complementary double strand or single strand of nucleic acids, detection sensitivity to a target nucleic acid can be increased, and diagnosis and detection reliability can be improved twice through in-situ determinations. Through simultaneous single nucleotide polymorphism (SNP) detection and expression profile determination, more accurate diagnosis for many diseases can be achieved. The assay device can be easily modified to be suitable for detection with general laser-based detection systems such as CD-ROM readers. Information read from the assay device is digitized as software and transmitted to and received by doctors and patients through a computer network or wirelessly, which enables construction of remote diagnosis systems. | 06-10-2010 |
20100151561 | Automatic Microfluidic Processor - In a microfluidic processor with integrated active elements for handling process media, the active elements act by changes in their volume, swelling degree, material composition, their strength and/or viscosity. The procedures to be performed are defined already by the constructive configuration of the microfluidic processor by an appropriate logic connection of the individual active elements defined in their function, by the sequence of the temporal activation of the individual elements, and with respect to their processing speed and their precision. The process is enabled by action of a substantially non-directional collectively acting environmental parameter, in particular, the presence of a solvent or environmental temperature or both. | 06-17-2010 |
20100159575 | QUANTITATIVE SAMPLER OF PATHOGENS IN EXHALED AIR - A quantitative sampler of pathogens in exhaled air is provided. The quantitative sampler includes an air inputting pipe for a sampled individual to blow exhaled air; a check valve unit for preventing the exhaled air from reversely flowing; an aerosol filtering unit having an aerosol filtering material for collecting aerosol particles in the exhaled air; and an air flow meter for measuring if total accumulated volume of the exhaled air reaches a predetermined sampling volume defined as a concentration denominator of exhaled pathogens. The quantitative sampler can be used to collect the aerosol particles, and then moves the aerosol filtering material out of the aerosol filtering unit followed by separating genetic substances in the aerosol particles from the aerosol filter, so that a real-time qPCR can be reacted by using the genetic substances for obtaining a quantitative concentration of the exhaled pathogens. | 06-24-2010 |
20100167386 | IMMUNOSENSOR SYSTEM FOR BLOOD WITH REDUCED INTERFERENCE - An electrochemical immunosensor system with reduced interference, comprising: a first immunosensor that generates an electrochemical signal based on the formation of a sandwich between an immobilized antibody, a target analyte and a labeled antibody, wherein a portion of the signal arises from non-specific binding of the labeled antibody in the region of the first immunosensor, and
| 07-01-2010 |
20100173393 | MICROFLUIDIC VALVE AND METHOD OF MAKING SAME - The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve. | 07-08-2010 |
20100173394 | Droplet-based assay system - Systems, including apparatus and methods, for performing assays. These systems may involve separating sample components by partitioning them into droplets or other partitions, amplifying or otherwise reacting the components within the droplets, detecting the amplified components, or characteristics thereof, and/or analyzing the resulting data, among others. | 07-08-2010 |
20100173395 | MICROFLUIDIC DEVICES FOR FLUID MANIPULATION AND ANALYSIS - The present invention relates to microfluidic devices and methods for manipulating and analyzing fluid samples. The disclosed microfluidic devices utilize a plurality of microfluidic channels, inlets, valves, filter, pumps, liquid barriers and other elements arranged in various configurations to manipulate the flow of a fluid sample in order to prepare such sample for analysis. | 07-08-2010 |
20100173396 | AMPEROMETRIC IMMUNOSENSOR - An electrochemical immunosensor system with reduced interference, comprising: a first immunosensor that generates an electrochemical signal based on the formation of a sandwich between an immobilized antibody, a target analyte and a labeled antibody, wherein a portion of the signal arises from non-specific binding of the labeled antibody in the region of the first immunosensor, and
| 07-08-2010 |
20100173397 | Dual Path Immunoassay Device - The systems of the invention include test cells with a first sorbent material defining a first flow path for a solution, a second sorbent material defining a second flow path distinct from the first flow path for a sample, and a test line or test site with immobilized antigens or antibodies or other ligand binding molecules such as aptamers, nucleic acids, etc. located at the junction of the first and second sorbent materials. The first and second sorbent strips touch each other at the test site location. | 07-08-2010 |
20100178690 | BIOMOLECULE DETECTION APPARATUS AND BIOMOLECULE MEASUREMENT SYSTEM - The present invention relates to a biomolecule detection apparatus and a biomolecule measurement system. A biomolecule detection apparatus according to an aspect of the invention may include: an upper disc having a fluid inlet in a thickness direction through which a fluid is introduced to the inside; a lower disc laminated to the upper disc and having a fluid outlet in a thickness direction through which the fluid exits to the outside; detection units provided on each of the upper disc and the lower disc and including spherical microbeads having surfaces coated with materials used to capture biomolecules; and via holes provided along the edge of each of the upper disc and the lower disc so that the fluid flows between the upper disc and the lower disc. | 07-15-2010 |
20100178691 | Systems, Methods, and Apparatus for Single Molecule Sequencing - An embodiment generally relates to a system for analysis of an analyte. The system can include a transparent substrate. The system also includes an excitation light source configured to induce an evanescent wave excitation of a fluorescently labeled molecule near the access to the transparent substrate and a detector for detecting the fluorescently labeled molecule. | 07-15-2010 |
20100184202 | Thermal Reaction Device and Method for Using the Same - An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block. | 07-22-2010 |
20100197000 | MEMBRANE ARRAY AND ANALYTICAL DEVICE - A membrane array used to detect one or more analytes from a small sample of fluid with high sensitivity is provided. The membrane array can be employed in various analytical devices and is especially useful for identifying analytes from whole blood with minimal or negligible background interference. | 08-05-2010 |
20100197001 | GENE DETECTION FIELD-EFFECT DEVICE AND METHOD OF ANALYZING GENE POLYMORPHISM THEREWITH - A gene detection field-effect device provided with an insulation film ( | 08-05-2010 |
20100197002 | Systems for genome selection - Systems, methods, compositions and apparatus relating to genome selection are disclosed. | 08-05-2010 |
20100203622 | Method for Determining the Phenotype of Cells - The present invention provides a system, a device, and a kit for comparing the phenotypic state of a first unknown sample material with a sample material of a known phenotypic state. The present invention also provides a method of using the system, device and kit. | 08-12-2010 |
20100210009 | OPTICAL MICROLABELS: SHAPES AND REFLECTORS - Labels and methods of producing labels for use in clinical, analytical and pharmaceutical development assays are provided. Labels may comprise shape-encoded particles which may be coupled to ligands such as DNA, RNA and antibodies, where different shapes are used to identify which ligand(s) are present. Labels may also comprise reflectors, including retroreflectors and retroreflectors susceptible to analyte-dependent assembly for efficient homogeneous assays. | 08-19-2010 |
20100216226 | Device, system, and method for controllably reducing inflammatory mediators in a subject - Devices, systems, and methods are provided for controlling an inflammatory response in a subject. Devices, systems, and methods are provided that alter the functional structure of one or more inflammatory mediators in the peripheral blood of the subject. The device or system is useful in a method for treating an inflammatory disease or condition in the subject. | 08-26-2010 |
20100216227 | Rotatable Sample Disk and Method of Loading a Sample Disk - A rotatable sample disk configured for samples of biological material. The sample disk may include a fill chamber for storing a first biological material, a plurality of first sample chambers positioned in the sample disk farther from the rotational axis of the sample disk than the fill chamber, a plurality of second sample chambers, and a plurality of circumferential fill channels. Each of the second sample chambers may be configured to permit fluid communication with a respective first sample chamber. The plurality of circumferential fill conduits may be configured to permit transfer of the first biological material from the fill chamber to the plurality of first sample chambers upon a first rotation of the sample disk about the rotational axis. Methods of loading a plurality of sample chambers in a sample disk are also provided. | 08-26-2010 |
20100221816 | REACTION KIT - Disclosed is a reaction kit for preventing the entry of foreign matter into a reaction plate from the outside and the pollution of a surrounding environment. The reaction kit includes: a reaction plate ( | 09-02-2010 |
20100227383 | GENE DETECTION AND DETERMINATION APPARATUS, GENE REACTOR, AND INCUBATOR - A genetic detection and determination apparatus which detects or determines genetic information, provided with a reaction container having a reaction vessel, including: a container set portion on which the reaction container is set; a moving mechanism which moves the container set portion along a track; a reaction vessel dividing portion which divides the reaction vessel into a plurality of reaction chambers by deforming the reaction vessel; a heating and cooling portion which heats and cools the reaction container; and a measuring portion which is movable above the container set portion in parallel to an upper surface of the reaction container provided on the container set portion and which measures a reaction within each of the reaction chambers. | 09-09-2010 |
20100227384 | Portable Genomic Analyzer - An apparatus for identifying a target portion of a sample. In some embodiments, the sample can be substantially purified and/or amplified in a test chamber. In some embodiments, the sample can be provided in an amount so that amplification is not necessary. The apparatus generally comprises a test chamber having an input region for receiving a sample and an analysis region. A sample chamber can be defined by at least a portion of the test chamber. A separation mechanism can separate a target portion of the sample from the sample and an analysis chamber can aid in analyzing at least the target portion of the sample. | 09-09-2010 |
20100240121 | Electrospun nanofibrous membrane assembly for use in capturing chemical and/or biological analytes - A membrane assembly adapted for use in capturing an analyte of interest, the membrane assembly comprising in one embodiment (a) an electrospun nanofibrous membrane, the electrospun nanofibrous membrane comprising a random mat of electrospun nanofibers, at least some of the electrospun nanofibers including one or more types of functional groups; and (b) at least one molecular recognition element immobilized on the random mat via a functional group, the molecular recognition element being adapted to selectively bind the analyte of interest. The membrane assembly may be incorporated into a sensor. | 09-23-2010 |
20100240122 | Spotter provided with spot pattern encryption function and detection device coping with spot pattern encryption - Plural probes spotted on a probe immobilization substrate are arranged such that the probes cannot be specified by a third party easily. When the plural probes are spotted on the probe immobilization substrate, a position where each probe is spotted is changed for each probe immobilization substrate to be prepared, whereby types of probes to be arranged in respective spot addresses are encrypted. The preset invention provides a spotter, a dispensing device to be used for the spotter, a probe immobilization substrate that is prepared using the spotter, and a detection device that decodes encrypted positions where the respective probes are spotted. | 09-23-2010 |
20100240123 | Apparatus, System and Method for Purifying Nucleic Acids - Methods and devices for isolating nucleic acids from a mixture containing such nucleic acids and extraneous matter are provided. In one embodiment, the method of the invention comprises passing the mixture through a glass frit under conditions effective to separate the nucleic acids from the extraneous matter. In a more specific embodiment, the glass frit is a sintered glass frit. | 09-23-2010 |
20100248348 | TARGET NUCLEIC ACID MEASURING APPARATUS - The present invention relates to calculation of an amount equivalent to an initial template amount in a test sample by fitting a theoretical expression to an amount equivalent to an amplification amount of target nucleic acid for each thermal cycle number, wherein the theoretical expression includes an environmental coefficient as an exponent parameter and a parameter of the amount equivalent to the initial template amount, and includes at least one parameter among an amount equivalent to a saturation amount upon the target nucleic acid amplification, a reaction acceleration coefficient, and a reaction inhibition coefficient. | 09-30-2010 |
20100248349 | ANIMAL BLOOD CELL MEASURING APPARATUS - An animal blood cell measuring apparatus comprising: a specimen preparation section for preparing a measurement specimen from blood of an animal; a characteristic information obtaining section for obtaining characteristic information indicating a characteristic of the measurement specimen, from the measurement specimen prepared by the specimen preparation section; and a controller configured for performing operations comprising: (a) classifying aggregate reticulocytes contained in the blood from other blood cells, based on the characteristic information obtained by the characteristic information obtaining section; and (b) outputting information regarding a number of the classified aggregate reticulocytes. | 09-30-2010 |
20100267124 | BIO-ANALYSIS SYSTEM - To improve operability from collection through to gene analysis, and enable the detection of microorganisms in a short time with good accuracy. Provided is an analysis device | 10-21-2010 |
20100273247 | SEMI-QUANTITATIVE IMMUNOLOGICAL DETECTION DEVICE WITH DIFFERENTIAL ANTIBODIES OR SUBSTRATES - A semi-quantitative immunological detection device with differential antibodies and substrates is provided. The semi-quantitative immunological detection device with differential antibodies, includes: a sample injecting portion through which a sample is injected; and a reaction portion where a plurality of reacting materials reacting with an MUT (material under test) contained in the sample with different sensitivities to generate marker materials are disposed in the same space. Accordingly, a diagnosis result can be visually identified with user's eyes, and the diagnosis result similar to that of a quantitative assay apparatus can be provided. | 10-28-2010 |
20100291666 | Molecular diagnostics system and methods - The present invention relates to automated devices and methods for the extraction of nucleic acids from cells, the amplification of segments of nucleic acid and the detection of nucleic acids, all in a convenient and portable manner. The invention is particularly suited for use in point-of-care medical diagnostic testing. | 11-18-2010 |
20100291667 | OPTICAL DETECTOR - Disclosed herein is an optical detector at least including: a first substrate in which a plurality of wells are formed; a second substrate in which a heating section is provided to heat the wells; a third substrate in which a plurality of photoirradiation sections are provided in alignment with the wells; and a fourth substrate in which a plurality of photodetection sections are provided in alignment with the wells. | 11-18-2010 |
20100291668 | Apparatus for Performing Amplicon Rescue Multiplex PCR - The present disclosure is a cassette for PCR amplification and detection of DNA amplicons, the cassette having a cassette pipette which may be moved vertically and horizontally within the cassette, at least one reagent chamber, and at least one detector, such as a microarray, for the detection of DNA amplified during the PCR amplification steps. | 11-18-2010 |
20100297744 | Multi-Channel Bioreactor with the Immobillization of Optical Sensing Membrane - The present invention relates to a multi-channel microbioreactor. More specifically, the present invention relates to a multi-channel microbioreactor having a number of wells in which an optical sensing membrane comprising a fluorescent dye and a bioconjugate, or a sensor material including a biomolecule conjugated to a fluorescent dye and a bioconjugate is formed. The multi-channel microbioreactor according to the present invention can be used for an in-situ optical detection of dissolved oxygen, carbon dioxide, pH, monosaccharides, polysaccharides, organic acids, alcohols, cholesterol, choline and xanthine, etc. | 11-25-2010 |
20100297745 | FLOW-THROUGH METHOD OF FUNCTIONALIZING INNER SURFACES OF A MICROFLUIDIC DEVICE - The present invention refers to a flow-through method of functionalizing an inner surface of a microfluidic device. The method can comprise transporting a silanizing solution through the microfluidic device to silanize the inner surface of the microfluidic device. The method can further comprise reacting the silanized surface with an oxidized polysaccharide to bind the oxidized polysaccharide to the silanized surface by transporting the oxidized polysaccharide through the microfluidic device. The present invention also refers to a microfluidic device which has been subjected to a flow-through method of the present invention so that at least a part of the inner surface or the entire inner surface of the microfluidic device is functionalized. | 11-25-2010 |
20100297746 | STANDING WAVE FIBERS FOR THE DETECTION OF NUCLEIC ACIDS - The present invention provides a standing wave fiber assembly for the collection and detection of a biological target in a complex biological fluid, including: an oscillator; and an elongated fiber coupled to the oscillator, wherein the elongated fiber is selectively exposed to a fluid potentially containing the biological target, and wherein the resonated elongated fiber attracts the biological target, and wherein a change in a response of the resonated elongated fiber indicates the presence of the biological target. The assembly also includes a top cover plate including one or more electrical connections and a port through which the fluid is introduced. The assembly further includes a bottom cover plate including a well in which the fluid is contained. Optionally, the elongated fiber includes one or more probes homogenously functionalized along its length that bind targeted biological materials. | 11-25-2010 |
20100304470 | MICROFLUIDIC CHIP - A microfluidic chip including a substrate and at least a channel set is provided. The substrate has a surface, and the channel set is formed in the substrate and includes a channel, at a least a filler fillister, and at least a well fillister. The filler fillister and the well fillister are all connected to the channel, and the channel passes through the well fillister. The depth of the well fillister relative to the surface is longer than the depth of the channel relative to the surface. Hence, the well fillister can hinder the fluid flow in the channel and be used as a valve. | 12-02-2010 |
20100304471 | ASSAY - The present invention relates to test devices, and in particular devices capable of detecting the presence or absence of an analyte in a sample, such as a liquid sample. Also provided are methods of using such devices for quantitative or qualitative measurement of one or more analytes in a liquid sample. | 12-02-2010 |
20100311152 | Real-Time Amplification and Monitoring System Incorporating Vector-Based Algorithm for Analyzing Polynucleotide-Containing Samples - System for analyzing a polynucleotide-containing sample using real-time amplification and monitoring. The system includes a computer or processing device with software for performing a vector analysis of growth curves. The vector analysis advantageously simplifies polynucleotide quantitation by circumventing the need to establish thresholds used for calculating initiation of the growth phase, or to calculate derivatives. | 12-09-2010 |
20100311153 | METHOD AND DEVICE FOR PROCESSING A BIOLOGICAL FLUID FOR ANALYTE DETERMINATION - The present invention refers to a method of processing a biological fluid which comprises cellular components by heat treatment. The method is particularly useful for preparing biological samples for analyte detection. Further, the invention refers to a processed biological fluid comprising substantially quantitatively disintegrated cellular components. | 12-09-2010 |
20100317093 | FLEXIBLE POUCH AND CARTRIDGE WITH FLUIDIC CIRCUITS - Flexible pouch and flexible cartridge devices for fluid sample processing, related methods of making and using, related manufacturing systems and related instrumentation systems are described. Flexible pouches provide broad advantage in a wide variety of fields by overcoming the need for complex instrumentation, dedicated devices, and relatively high cost in conventional fluid sample devices. Flexible cartridge devices are particularly advantageous in control of fluid handling, rapid adaptation to a number of configurations by the end user, multiple uses for a single configuration, and in cost and ease of manufacture. | 12-16-2010 |
20100317094 | MILK ANALYSIS MICROFLUIDIC APPARATUS FOR DETECTING MASTITIS IN A MILK SAMPLE - A milk analysis apparatus for detecting mastitis in a milk sample by isolating somatic cells in the form of a pellet using centrifugal sedimentation is described. The apparatus comprises a vessel for holding the milk sample, and a centrifuge for rotating the vessel. The vessel includes an inlet for facilitating charging milk into a body portion of the vessel; and a trap for capturing somatic cells suspended in the milk sample. The somatic cells are biased towards the trap upon rotation of the vessel. | 12-16-2010 |
20100323432 | SAMPLE PROCESSING DEVICE FOR MICROCHIP - A sample processing device for a microchip, including: a sample vessel for packing a sample therein; and a reaction vessel which is continuous with the sample vessel through a channel, and to which the sample is sequentially delivered to be packed and mixed therein, in which the sample is repeatedly delivered between the sample vessel and the reaction vessel through the channel so that the sample is stirred and mixed. | 12-23-2010 |
20110003371 | DIAGNOSTIC DEVICES - The present invention relates to analytical methods, platforms, and devices for the rapid and efficient immunochromatic determination of one or more components in fluid samples. The devices are especially useful for identifying analytes in small volumes of whole blood samples utilizing one membrane principally for separating particles such as red blood cells from plasma and a second membrane as the site for reactions to identify the analytes. | 01-06-2011 |
20110008878 | TARGET NUCLEIC ACID MEASURING APPARATUS - The present invention relates to calculation of an initial template amount in a test sample by fitting a theoretical expression to detection intensity of target nucleic acid corresponding to thermal cycle number, wherein the theoretical expression includes an environmental coefficient as an exponent parameter, a parameter of the initial template amount in the test sample, and terms for internal standard correction and baseline correction of the detection intensity of the amplification amount of the target nucleic acid, and includes at least one parameter among a saturation amount upon the target nucleic acid amplification, a reaction acceleration coefficient, and a reaction inhibition coefficient. | 01-13-2011 |
20110014686 | Method and apparatus for imaging target components in a biological sample using permanent magnets - A system for enumeration of cells in fluids by image cytometry is described for assessment of target populations such as leukocyte subsets in different bodily fluids or bacterial contamination in environmental samples, food products and bodily fluids. Briefly, fluorescently labeled target cells are linked to magnetic particles or beads. In one embodiment, a small, permanent magnet is inserted directly into the chamber containing the labeled cells. The magnets are coated with PDMS silicone rubber to provide a smooth and even surface which allows imaging on a single focal plane. The magnet is removed from the sample and illuminated with fluorescent light emitted by the target cells captured by a CCD camera. In another embodiment, a floater having a permanent magnet allows the target cells to line up along a single imaging plane within the sample solution. Image analysis can be performed with a novel algorithm to provide a count of the cells on the surface, reflecting the target cell concentration of the original sample. | 01-20-2011 |
20110020918 | Microfluidic Assay Devices And Methods - A microfluidic device adapted to perform many simultaneous binding assays including but not limited to immunological experiments, such as ELISA assays, with minimal cross-talk between primary and secondary antibodies. | 01-27-2011 |
20110020919 | AUTOMATED LIQUID HANDLING DEVICE AND ASSOCIATED ASSAY UNIT - The present invention provides a novel system for efficiently and accurately analyzing targets in samples and for preparing samples for analysis with various analytical methods. A version of the present invention comprises a multi-function probe, configured for pipetting liquids directly, with a pipet tip, or with an assay unit described herein. Another version of the present invention includes an apparatus for conducting an immunoassay or selective adsorption separation comprising an assay unit and a multi-function probe. Another version of the present invention includes a multi-function probe and assay unit for use with an automated Cartesian robot. Other versions include one- or two-dimensional arrays comprising multi-function probes connected to syringe barrels for use with assay units. | 01-27-2011 |
20110020920 | MICROFABRICATED INTEGRATED DNA ANALYSIS SYSTEM - Methods and apparatus for genome analysis are provided. A microfabricated structure including a microfluidic distribution channel is configured to distribute microreactor elements having copies of a sequencing template into a plurality of microfabricated thermal cycling chambers. A microreactor element may include a microcarrier element carrying the multiple copies of the sequencing template. The microcarrier element may comprise a microsphere. An autovalve at an exit port of a thermal cycling chamber, an optical scanner, or a timing arrangement may be used to ensure that only one microsphere will flow into one thermal cycling chamber wherein thermal cycling extension fragments are produced. The extension products are captured, purified, and concentrated in an integrated oligonucleotide gel capture chamber. A microfabricated component separation apparatus is used to analyze the purified extension fragments. The microfabricated structure may be used in a process for performing sequencing and other genetic analysis of DNA or RNA. | 01-27-2011 |
20110020921 | Nucleic acid amplification reaction station for disposable test devices - An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber. | 01-27-2011 |
20110045579 | BIOSENSOR - Provided is a biosensor with precision, high accuracy, and high sensitivity capable of increasing measurement accuracy while allowing measurements to be performed anywhere at any time by anyone, and allowing measurement with a small amount of specimen. In a biosensor including a specimen application portion ( | 02-24-2011 |
20110059520 | Deoxyribonucleic acid measuring apparatus and method of measuring deoxyribonucleic acid - With an insulated gate field effect transistor in which deoxyribonucleic acid (DNA) probes are immobilized on a gold electrode, extension reaction on the gold electrode is performed with DNA polymerase to directly measure an increased amount of a phosphate group caused by the extension reaction, that is, negative charge, by means of a current change between a source and a drain of the insulated gate field effect transistor. Thus, presence/absence of hybridization of target DNAs with the DNA probes, and presence/absence of the extension reaction are detected. Optimum immobilization density of the DNA probes on the gold electrode is set at 4×10 | 03-10-2011 |
20110104794 | APPARATUS FOR PERFORMING BIOCHEMICAL PROCESSING USING CONTAINER HAVING WELLS - A biochemical processing apparatus includes a thermal cycle section, a processing section for performing a processing not requiring heating or cooling, and a cooling section. These sections are arranged in that order and opposed to a container with a plurality of wells. | 05-05-2011 |
20110111487 | MEHTOD AND APPARATUS FOR ASSAY BASED ON LIGHT DIFFRACTION - The present invention relates to a method and apparatus for detecting analytes in a medium, and more particularly the present invention relates to an assay based on light diffraction which appears or changes upon the binding of analytes to their specific receptors laid out in patterns on a substrate, which has high sensitivity due to the appropriate choice of such patterns. The present invention is based on the principle that the pattern of recognition elements, which gives rise to the diffraction of the incident light in a diffraction-based assay, can be chosen in such a way so as to facilitate detection, and to enhance the signal to be detected compared to known gratings such as parallel straight lines. In one aspect the substrate itself has a surface topography designed to enhance the diffraction pattern signals. In another aspect the substrate is a diffractive optic element having the analyte-specific receptors affixed to the optic element. In another aspect the diffractive optic element is used as a master stamp for producing patterns of analyte-specific receptors which give the signal enhancements. | 05-12-2011 |
20110117636 | LATERAL FLOW IMMUNOASSAY DEVICE WITH A MORE RAPID AND ACCURATE TEST RESULT - The present invention provides a lateral flow immunoassay device for qualitative or quantitative analysis of an analyte of interest in a whole blood sample with a more rapid and accurate result. This device includes a sample receiving pad, a conjugate pad, a flow delaying pad and a wicking membrane in this order. A polycation is provided within and bound to the sample receiving pad in order to separate the red blood cell from the whole blood sample. A relation between average pore size (P | 05-19-2011 |
20110117637 | ZERO-MODE WAVEGUIDES WITH NON-REFLECTING WALLS - The application relates to improved optical containment structures, methods of manufacture and use, and systems for employing same. The optical containment structures generally comprise zero-mode waveguide structures having non-reflective walls. The non-reflective walls allow the preparation of optical containment regions in which the optical containment dimensions can be decoupled from the solution containment dimensions. The application also relates to methods for producing islands of functionality within nanoscale apertures. | 05-19-2011 |
20110136216 | DIAGNOSTIC KIT FOR RESPIRATORY DISEASE - The present invention relates to a diagnostic kit for a respiratory disease that enables a preprocessing process with respect to a sample to be performed within a diagnostic kit when diagnosing respiratory disease using the diagnostic kit, and thereby enables all the operations starting from collecting the sample to verifying a diagnosis result to be automatically performed within the diagnostic kit. The diagnostic kit for a respiratory disease may perform a field diagnosis at anywhere without being restricted to a specific location, and even a general user may also easily obtain an accurate diagnosis result. | 06-09-2011 |
20110151549 | Device For Carrying Out An Individual Immunoassay In A Fully Automatic Manner - The invention relates to a device for carrying out an individual immunoassay in a fully automatic manner in order to detect the presence of a biologically active substance in a sample, and to the use of one such device. The invention also relates to a sample strip which, in order to carry out an individual immunoassay for the detection of a biologically active substance in a sample using the above-mentioned device, comprises a plurality of cavities for providing the reagents required for the assay. The sample strip is sealed with a film carrying a code specific to the assay. | 06-23-2011 |
20110151550 | System For Multi Color Real Time PCR - The invention is directed to a system for performing multi-color real time PCR, comprising a flexible real time PCR instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixture which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm. | 06-23-2011 |
20110159579 | THERMAL CYCLING REACTION BLOCK AND CONTINUOUS REAL-TIME MONITORING APPARATUS USING THE SAME - Disclosed is real-time monitoring apparatus comprising a thermal cycling reaction block having heating block which is formed of a hollow part and divided by an insulating layer, and a capillary tube through which a sample is flowed in and/or out and which is wound on the heating block so that the different temperatures are transferred and thus reaction cycle is repeatedly performed; a light source; a band pass filter; a condensing lens; a beam splitter; a reflecting mirror which is rotatably connected with a motor so as to transfer the excitation light reflected from the beam splitter to the capillary tube and reflect the fluorescence generated from the sample in the capillary tube; and a fluorescence detecting part. | 06-30-2011 |
20110165665 | APPARATUS AND METHODS FOR DETECTING DNA IN BIOLOGICAL SAMPLES - Apparatus and methods are described for detecting target DNA in a biological sample using capture probes and electrically-assisted hybridization. The reaction cell is formed with an attachment surface of aluminum oxide for better thermal and physical properties, and the aluminum oxide surface is coated with anti-DIG antibody to provide a convenient attachment layer for the capture probes allowing their correct orientation, while the capture probes are formed with a DIG-label so that they attach to the surface of the cell through an anti-DIG/DIG linkage. | 07-07-2011 |
20110177585 | RAPID DETECTION NANOSENSORS FOR BIOLOGICAL PATHOGENS - An apparatus for the rapid detection of multiple pathogens using a FRET-based phenomenon. A volume of fluid, possibly containing pathogens, is passed through an intake and combined with an assay solution of quantum dot/antibody-antigen/quencher complexes that dissociate and recombine with the pathogens into quantum dot/antibody-pathogen complexes. The quantum dot/antibody-antigen/quencher and quantum dot/antibody-pathogen complexes are captured on a detection filter which is illuminated by a light source. The quantum dot/antibody-pathogen complexes, but not the quantum dot/antibody-antigen/quencher complexes, fluoresce when excited by the light from the light source and the fluorescence is picked up by a photodetector, indicating the presence of the pathogens. | 07-21-2011 |
20110177586 | DEVICE AND METHOD FOR PRESSURE-DRIVEN PLUG TRANSPORT - The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid. | 07-21-2011 |
20110183409 | SEALED CHIP PACKAGE - The invention provides environmental packages, instruments, and methods for sealing, protecting, and providing analysis chips for processing and analysis. The analysis chips are bonded directly or indirectly to chip carriers which are held within the chambers of an environmental packaging strip. The chambers are sealed with a sealing film such that the chip carriers can be extracted using a piercing tool and an extraction tool. | 07-28-2011 |
20110189763 | KIT AND METHOD FOR EVALUATING DETECTION PROPERTIES IN AMPLIFICATION REACTIONS - The invention relates to a kit comprising, (a) a first group of containers, comprising two or more containers with human genomic DNA, wherein the human genomic DNA in each of the two or more containers is of different human origin and the amount of DNA in the container is defined, (b) a second group of containers, comprising two or more containers with human genomic DNA, wherein the concentration of the DNA varies between the containers and the concentration of the DNA in the container is defined and (c) a third group of containers, comprising two or more containers with human genomic DNA, wherein the human genomic DNA in each container is a mixture of genomic DNA and is from at least two different individuals. The invention also relates to an evaluation tool and methods of use of the kit. | 08-04-2011 |
20110195491 | DEVICES AND SYSTEMS FOR BIOMARKER DETECTION - Devices and systems for biomarker detection. In at least one embodiment of a device for analyzing the presence or absence of a diagnostic marker to be detected in a body fluid, the device comprises a container, wherein the container houses a sample reservoir having a sample input port in fluid connection with at least one detector array, wherein the detector array comprises at least one detector comprising a reaction chamber comprising an immobilized capture molecule, a stabilizing agent contained within the sample reservoir, the stabilizing agent useful to completely or substantially prevent degradation or inactivation of a diagnostic marker, and a reagent contained within the detector array, the reagent capable of undergoing a colorimetric reaction or displaying an optically detectable signal and capable of reacting with the diagnostic marker. | 08-11-2011 |
20110201099 | Assay Cartridges and Methods of Using the Same - Assay cartridges are described that have a detection chamber, preferably having integrated electrodes, and other fluidic components which may include sample chambers, waste chambers, conduits, vents, bubble traps, reagent chambers, dry reagent pill zones and the like. In certain embodiments, these cartridges are adapted to receive and analyze a sample collected on an applicator stick. Also described are kits including such cartridges and a cartridge reader configured to analyze an assay conducted using an assay cartridge. | 08-18-2011 |
20110212510 | Polarization-enhanced detector with gold nanorods for detecting nanoscale rotational motion and method therefor - A nanoscale motion detector attaches a gold nanorod ( | 09-01-2011 |
20110212511 | SYSTEM FOR DETECTING BIOMOLECULE WITH HIGH SENSITIVITY USING MICRO-CANTILEVER - Provided is a protein detection system using a micro-cantilever and based on immune responses, wherein the micro-cantilever shows significantly improved sensitivity to allow detection of a trace amount of biomolecule. To the micro-cantilever, sandwich immunoassay is applied, and the sandwich immunoassay uses a polyclonal antibody or silica nanoparticles having a monoclonal antibody bound thereto, so that variations in the output signals of the cantilever are amplified and the detection sensitivity is significantly improved. The system enables detection of disease specific antigen at several femtomolar levels, and makes it possible to detect a trace amount of protein related to diseases, particularly to cancers, with ease. | 09-01-2011 |
20110223652 | PIEZOELECTRIC-BASED NANOPORE DEVICE FOR THE ACTIVE CONTROL OF THE MOTION OF POLYMERS THROUGH THE SAME - Apparatus, system, and methods are provided for utilizing piezoelectric material for controlling a polymer through a nanopore. A reservoir is formed filled with conductive fluid. A membrane is formed that separates the reservoir. A nanopore is formed through the membrane. The membrane comprises electrical conductive layers, piezoelectric layers, and insulating layers. The piezoelectric layers are operative to control a size of the nanopore for clamping/releasing a polymer as well as to control the thickness of part of the membrane when a voltage is applied to the piezoelectric layers. Combinations of clamping/releasing the polymer and changing the thickness of part of the membrane can move a polymer through the nanopore at any electrically controlled speed and also stretch or break a polymer in the nanopore. | 09-15-2011 |
20110223653 | Method and apparatuses for sorting objects in forensic DNA analysis and medical diagnostics - The present invention relates to an apparatus and method of sorting objects and identifying the objects in a forensics sample, including using holographic optical trapping to sort objects from contaminants, and performing (single cell) PCR-based STR analysis on the objects to determine their identification. In addition, the chip used as a support for sorting the objects can also be used for performing single cell PCR-based STR analysis. In another embodiment, a microfluidics chip is used to stream the sample and sort the objects, before single cell PCR-based STR analysis is performed. The chip used for sorting utilizing HOT in the absence or presence of microfluidic streaming and sorting can also be the same as that used for the single cell PCR-based STR analysis. | 09-15-2011 |
20110236964 | DNA Sequencing System - An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector. | 09-29-2011 |
20110256618 | Nucleic acid sequencing methods and systems - Sequencing methods that use an exonuclease that comprises template dependent nucleobase binding activity are provided. Related compositions and sequencing systems are also provided. | 10-20-2011 |
20110269222 | Immunoassay Device with Improved Sample Closure - An apparatus and method for sealing a fluid sample collection device, comprising: loading a fluid sample collection device with a fluid sample, said device comprising a housing having at least one substantially planar surface that includes an orifice in fluid communication with an internal fluid sample holding chamber which terminates at an internal capillary stop; and slidably moving a sealing element over at least a portion of said substantially planar surface in a way that displaces any excess fluid sample away from the orifice, seals the fluid sample within said holding chamber, and inhibits the fluid sample from prematurely breaking through the internal capillary stop. | 11-03-2011 |
20110275144 | DEVICES AND METHODS FOR DUAL EXCITATION RAMAN SPECTROSCOPY - Spectroscopic analysis systems and methods for analyzing samples are disclosed. An analysis system may contain an electromagnetic radiation source to provide radiation, a spectroscopic analysis chamber to perform a coherent Raman spectroscopy (e.g., stimulated Raman or coherent anti-Stokes Raman spectroscopy), and a radiation detector to detect radiation based on the spectroscopy. The chamber may have a resonant cavity to contain a sample for analysis, at least one window to the cavity to transmit the first radiation into the cavity and to transmit a second radiation out, a plurality of reflectors affixed to a housing of the cavity to reflect radiation of a predetermined frequency, the plurality of reflectors separated by a distance that is sufficient to resonate the radiation. The spectroscopic analysis system may be coupled with a nucleic acid sequencing system to receive a single nucleic acid derivative in solution and identify the derivative to sequence the nucleic acid. | 11-10-2011 |
20110294199 | APPARATUS FOR POINT-OF-CARE DETECTION OF NUCLEIC ACID IN A SAMPLE - Provided herein are methods and apparatus for detecting a target nucleic acid in a sample and related methods and apparatus for diagnosing a condition in an individual. The condition is associated with presence of nucleic acid produced by certain pathogens in the individual. | 12-01-2011 |
20110294200 | VIRTUAL SEPARATION OF BOUND AND FREE LABEL IN A LIGAND ASSAY FOR PERFORMING IMMUNOASSAYS OF BIOLOGICAL FLUIDS, INCLUDING WHOLE BLOOD - Detection and characterization of immunologically detected substances are performed electronically on human and animal biological fluids such as whole blood, serum, plasma, urine, milk, pleural and peritoneal fluids, and semen, which fluids are contained in a thin chamber forming a quiescent fluid sample, which chamber has at least two parallel planar walls, at least one of which is transparent. | 12-01-2011 |
20110300620 | Combo-tip Rack - The invention relates to a three-part rack for holding re-usable pipette tips. The rack comprises an upper, a lower and an insert rack. The rack is optimized for holding first and second types of pipette tips, and comprises contamination protection. | 12-08-2011 |
20110300621 | Process head positioning - A method is disclosed for mechanically aligning a process device and a rack which comprises mechanical engagement of positioning elements on the process device and positioning elements on the consumable. | 12-08-2011 |
20110300622 | Apparatus and Method for Transporting Sample Well Trays - An apparatus for transporting sample well trays with respect to a heating device is provided. The apparatus includes a sample well tray holder, a rotational actuator, and a biasing mechanism. The sample well tray holder includes a plate in which a sample well tray may be positioned. The sample well tray holder is configured to rotate about a first rotational axis. The rotational actuator is configured to rotate the sample well tray holder about the first rotational axis. The biasing mechanism is configured to urge the sample well tray holder in a generally upward direction along the first rotational axis. | 12-08-2011 |
20110306118 | Consumable hardware coding system - An analytical system is disclosed which recognizes different types of consumables with an identical footprint based on a unique surface geometry of each consumable. The analytical system comprises stackers into which said consumables can be loaded either automatically or by the user. The stacker for one specific type of consumable recognizes said type of consumable based on its unique surface geometry. | 12-15-2011 |
20110306119 | SYSTEMS AND METHODS FOR MONITORING THE AMPLIFICATION AND DISSOCIATION BEHAVIOR OF DNA MOLECULES - The present invention relates to systems and methods for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules. The present invention in one embodiment provides a system that includes a microfluidic channel comprising a PCR processing zone and an HRTm analysis zone; and an image sensor having a first image sensor region having a first field of view and a second image sensor region having a second field of view, wherein the second field of view is different than the first field of view, wherein at least a portion of the PCR processing zone is within the first field of view; and at least a portion of the HRTm analysis zone is within the second field of view. | 12-15-2011 |
20110306120 | ASSAY DEVICE AND METHODS - The present invention relates to the use of amphipathic polymers to enhance lateral flow and reagent mixing on assay devices. More specifically, the invention relates to use of an amphipathic polymer in assay methods including a device for determining the concentration of lipids in blood serum or plasma. | 12-15-2011 |
20110312071 | MICROFLUIDIC DEVICE WITH LARGE CHANNELS FOR CELL TRANSPORT AND SMALL CHANNELS SUITABLE FOR BIOCHEMICAL PROCESSES - A microfluidic device for molecular diagnostic analysis of a biological sample containing cells, the microfluidic device having a supporting substrate, a plurality of microsystems technology (MST) channels each with a cross-sectional area between 1 square micron and 400 square microns for biochemical processing of constituents within the biological sample, and, a plurality of cell transport channels, each with a cross-sectional area greater than 400 square microns for receiving the biological sample and transporting cells within the biological sample to predetermined sites in the MST channels. | 12-22-2011 |
20110312072 | MICROFLUIDIC DEVICE WITH SURFACE MICRO-MACHINED CHIPS AND INTERCONNECTING CAP - A microfluidic device for diagnostic analysis of a sample fluid, the microfluidic device having a plurality of lab-on-a-chip (LOC) devices each having a supporting substrate and a microsystems technology (MST) layer for processing the sample fluid, and, a cap interconnecting the plurality of LOC devices, the cap having a cap channel for establishing fluid communication between the MST layers on at least two of the LOC devices. | 12-22-2011 |
20110312073 | MICROFLUIDIC TEST MODULE INCORPORATING SURFACE MICRO-MACHINED CHIPS AND INTERCONNECTING CAP - A test module having a casing for hand held portability, a receptacle for receiving a sample fluid, and, a microfluidic device in fluid communication with the inlet, the microfluidic device having a plurality of lab-on-a-chip (LOC) devices and a cap in engagement with each of the plurality of LOC devices, wherein, the cap is configured to establish fluid communication between at least two of the LOC devices. | 12-22-2011 |
20110312074 | MICROFLUIDIC TEST MODULE WITH SAMPLE RECEPTACLE - A test module having a receptacle for receiving an unprocessed biological sample through an opening, a cover movable between open and closed positions, the cover exposing the opening when in the open position and closing the opening when in the closed position, and, a microfluidic device for processing the biological sample, the microfluidic device having a sample inlet, wherein, the receptacle is configured for fluid communication with the sample inlet such that the biological sample flows to the sample inlet by capillary action. | 12-22-2011 |
20110312075 | LOC DEVICE WITH PARALLEL INCUBATION AND PARALLEL DNA AND RNA AMPLIFICATION FUNCTIONALITY - A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample containing genetic material including DNA and RNA, a supporting substrate, a plurality of reagent reservoirs, a first incubation section, the first incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, a second incubation section, the second incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material in parallel with the first incubation section, a first nucleic acid amplification section downstream of the first incubation section for amplifying at least some of the genetic material, and, a second nucleic acid amplification section downstream of the second incubation section for amplifying at least some of the genetic material in parallel with the first nucleic acid amplification section, wherein, the first incubation section, the second incubation section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate. | 12-22-2011 |
20110312076 | MICROFLUIDIC TEST MODULE WITH FLEXIBLE MEMBRANE FOR INTERNAL MICROENVIRONMENT PRESSURE-RELIEF - A microfluidic test module having an outer casing for hand held portability, and, a microfluidic device mounted within the outer casing for processing a biological sample, the outer casing providing a microenvironment adjacent the microfluidic device, wherein, the outer casing has a membrane seal of flexible material between the microenvironment and atmosphere for reducing pressure changes in the microenvironment resulting from atmospheric pressure fluctuations. | 12-22-2011 |
20110312077 | LOC DEVICE FOR ELECTROCHEMILUMINESCENT DETECTION OF TARGET NUCLEIC ACID SEQUENCES IN A FLUID WITH CALIBRATION CHAMBER CONTAINING PROBES DESIGNED TO BE NON-COMPLEMENTARY WITH ANY NUCLEIC ACID SEQUENCES IN THE FLUID - A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having hybridization chambers containing electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, at least one calibration chamber containing ECL probes designed to be non-complementary to any nucleic acid sequence in the fluid, and, electrodes for receiving an electrical pulse to excite the ECL luminophores. | 12-22-2011 |
20110312078 | MICROFLUIDIC DEVICE FOR DETECTING TARGET NUCLEIC ACID SEQUENCES IN MITOCHONDRIAL DNA - A microfluidic device for detecting mitochondrial DNA in a sample, the microfluidic device having an inlet for receiving a sample of biological material having cells with mitochondria containing mitochondrial DNA, a lysis section for lysing the mitochondria to release the mitochondrial DNA, probes for hybridization with target nucleic acid sequences in the mitochondrial DNA to form probe-target hybrids, and, a photosensor for detecting the probe-target hybrids. | 12-22-2011 |
20110312079 | LOC WITH DIGITAL MEMORY TO STORE EPIDEMIOLOGICAL UPDATES - A lab-on-a-chip (LOC) device for analyzing a sample fluid, the LOC device having a sample inlet for receiving the sample, a microsystems technology (MST) layer with functional sections for processing and analyzing the sample, and, CMOS circuitry with digital memory for storing epidemiological data, and configured to download epidemiological data updates from an external source. | 12-22-2011 |
20110318820 | Immobilized Buffer Particles and Uses Thereof - The disclosure relates to novel particle compositions and methods of making said compositions having applications in nucleic acid analysis, as well as apparatuses and systems for the same. | 12-29-2011 |
20110318821 | BIOAGENT DETECTION METHODS - The present invention relates the use of broad range primer (e.g., as broad range capture olignucleotides) immobilized in a SCODA method gel to allow, for example, selective concentration of target nucleic acids. Such concentrated target nucleic acids may, for example, be: i) eluted from the gel and analyzed (e.g., by broad range primer methods); ii) subject to in situ (e.g., in gel) PCR methods; and/or iii) analyzed in the gel (e.g., by fluorescent detection methods). | 12-29-2011 |
20120003727 | Immunoassay Device for Detecting Antibodies and Antigens - A fourth generation immunoassay device includes first, second, third, and fourth sorbent or bibulous materials defining first, second, third and fourth horizontal flow paths. The first and second flow paths are for migration of first and second conjugates while the third and fourth flow paths are for the migration of a liquid sample. A first test area for detecting the presence of one or more different antibodies is located at the juncture of the first and third flow paths, and a second test area for detecting the presence of one or more different antigens is located at the juncture of the second and fourth flow paths. A housing is optionally provided for the sorbent materials with an opening for receiving a sample, one or more openings for receiving buffer solution or a conjugate-buffer subcomplex. The housing may also have viewing windows above the detection areas. | 01-05-2012 |
20120009665 | METHODS OF PRODUCING MULTI-LAYERED MICROFLUIDIC DEVICES - Microfluidic devices are prepared by providing a substrate material having a solid adhesive thin sheet, printing solid ink on the substrate using a conventional printer, selectively etching the substrate using a wax masking layer to obtain a desired pattern, removing the masking layer from the substrate, aligning and bonding together the pattern of the substrate to a pattern of a second substrate to form a layer of substrates, and curing the layer of substrates to result in a three-dimensional microfluidic device. | 01-12-2012 |
20120009666 | METHOD AND APPARATUS FOR ANTIGEN RETRIEVAL PROCESS - The invention provides a method for antigen retrieval of a formaldehyde-fixed tissue sample comprising incubating a formaldehyde-fixed tissue sample in a first antigen retrieval solution at a temperature of greater than 90° C., transferring the tissue sample to a second antigen retrieval solution, and incubating the tissue sample in the second antigen retrieval solution at a temperature of greater than 90° C. The invention also provides a kit and sample delivery device for carrying out the method. | 01-12-2012 |
20120028344 | IMMUNODIAGNOSTIC TEST CARDS HAVING INDICATING INDICIA - An immunodiagnostic test card includes a plurality of transparent chambers wherein each chamber includes a quantity of testing material that combines with a patient sample, when mixed, to produce an agglutination reaction. A plurality of indicia are disposed to aid in the manufacture and determining the usability of the cards prior to test and also in objectively grading the agglutination reactions that are formed or lack of agglutination. | 02-02-2012 |
20120034684 | MAGNETIC IMMUNOSENSOR WITH TRENCH CONFIGURATION AND METHOD OF USE - The present invention provides apparatus and methods for the rapid determination of analytes in liquid samples by immunoassays incorporating magnetic capture of beads on a sensor capable of being used in the point-of-care diagnostic field. | 02-09-2012 |
20120034685 | ASSAY SYSTEMS FOR DETERMINATION OF SOURCE CONTRIBUTION IN A SAMPLE - The present invention provides assay systems and methods for detection of copy number variation at one or more loci and polymorphism detection at one or more loci in a mixed sample from an individual. | 02-09-2012 |
20120034686 | DEVICES AND METHODS FOR DUAL EXCITATION RAMAN SPECTROSCOPY - Spectroscopic analysis systems and methods for analyzing samples are disclosed. An analysis system may contain an electromagnetic radiation source to provide radiation, a spectroscopic analysis chamber to perform a coherent Raman spectroscopy (e.g., stimulated Raman or coherent anti-Stokes Raman spectroscopy), and a radiation detector to detect radiation based on the spectroscopy. The chamber may have a resonant cavity to contain a sample for analysis, at least one window to the cavity to transmit the first radiation into the cavity and to transmit a second radiation out, a plurality of reflectors affixed to a housing of the cavity to reflect radiation of a predetermined frequency, the plurality of reflectors separated by a distance that is sufficient to resonate the radiation. The spectroscopic analysis system may be coupled with a nucleic acid sequencing system to receive a single nucleic acid derivative in solution and identify the derivative to sequence the nucleic acid. | 02-09-2012 |
20120034687 | PROTECTION OF BIOANALYTICAL SAMPLE CHAMBERS - An apparatus for performing bioanalytic processing and analysis, a bioanalytical reaction device, and a cartridge thereof are provided. The cartridge contains a housing and at least one sample chamber in a platform for storing biological samples, which the bioanalytical reaction device can process and analyze. The platform is movably connected to the housing such that the platform is movable between a stowed position, in which the sample chamber is protected by the housing, and an extended position, in which the sample chamber is outside of the housing. The bioanalytical reaction device includes an actuation device for moving the platform between the stowed and extended positions. | 02-09-2012 |
20120040445 | GAS-FREE FLUID CHAMBER - A Gas-free Fluid chamber for PCR. The present invention relates to a device with a fluid chamber suitable for performing a polymerized chain reaction for gas-free filling. Such devices may be used in the field of e.g. molecular diagnostics. | 02-16-2012 |
20120040446 | PARTICLE AGGLUTINATION IN A TIP - An apparatus and a related method for performing particle agglutination reactions in at least one disposable probe tip are disclosed. The at least one probe tip includes a sample cavity for sample acquisition, at least one flanking cavity for the capture of particles by centrifugation or other means, a transition zone for the mixing of the sample with reagents for agglutination and a detection zone for the optical detection of particle agglutination. A mechanism may be attached to the probe tip for the controlled movement of fluids through the internal volume of the probe tip. The probe tip is particularly useful for the automation of high-throughput agglutination-type assays. | 02-16-2012 |
20120040447 | MAGNETIC IMMUNODIAGNOSTIC METHOD FOR THE DEMONSTRATION OF ANTIBODY/ANTIGEN COMPLEXES ESPECIALLY OF BLOOD GROUPS - The invention relates to a magnetic immunodiagnostic method for the demonstration of antibody-antigen complexes. One such method involves the research and/or identification of antibodies or antigens, preferably anti-antigen antibodies or antigens of a blood group, and comprises a suspension of magnetic particles coated with antigens that can be carried by cells such as erythrocytes. The invention also relates to a device and a kit for carrying out one such method. | 02-16-2012 |
20120058547 | METHOD AND SYSTEM FOR NUCLEIC ACID DETECTION USING ELECTROCONDUCTIVE OR ELECTROCHEMICALLY ACTIVE LABELS - A method for electrochemically or electrically detecting nucleic acids, utilizes electrochemically active or electrically conductive reporter materials. An electric voltage is applied and electric signals are measured to the electrodes that are suitable for detecting or quantifying the nucleic acid(s) in a sample. This technique is suitable for point-of-use applications, e.g. detecting bioanalytes in remote locations. A microchip, device, kit used adapted to be used for this method is also disclosed. | 03-08-2012 |
20120058548 | DETECTION OF BIOTARGETS USING BIORECEPTOR FUNCTIONALIZED NANOPARTICLES - A system for determining a presence of biomolecule targets includes a vessel holding a sample solution suspected of containing the biomolecule targets and a plurality of probes including bioreceptor functionalized metal nanoparticles including a plurality of metal atoms. The biomolecule targets when present in the sample solution bind to the plurality of probes. A separation device is for separating the plurality of probes into a first group of probes having the biomolecule targets bound thereto and a second group of probes not having the biomolecule targets bound thereto. A reagent is introduced via a port for breaking down the metal nanoparticles in the first group or second group of probes, wherein at least one signal moiety solution is formed. A device measures a parameter of the signal moiety solution. | 03-08-2012 |
20120064614 | DEVICE FOR PREPARING AND/OR TREATING A BIOLOGICAL SAMPLE - The invention relates to a device for preparing and/or treating a biological sample including an assembly ( | 03-15-2012 |
20120077259 | EPISTEM GENEDRIVE - We describe a thermal cycler comprising a Peltier-type thermoelectric element used for cooling a sample block, and a non-Peltier-type heating device for heating the sample block. The cycler also includes a heat sink connected to the Peltier-type element by a heat pipe, which permits thermal energy to transfer from the Peltier-type element to the heat sink. This configuration operates more efficiently than conventional thermal cyclers which use Peltier-type elements for heating and cooling, and allows a more rapid cycling time as well as operation in a wider range of ambient temperatures. Certain embodiments utilise the Peltier-type element as a thermal gate to reduce thermal loss during heating when the Peltier-type element is switched off. | 03-29-2012 |
20120077260 | RESERVOIR-BUFFERED MIXERS AND REMOTE VALVE SWITCHING FOR MICROFLUIDIC DEVICES - The present invention relates generally to the control of fluid flow rate and direction on a microfluidic device. In particular, the present invention provides an integrated valveless microfluidic device, where directional fluid control is controlled using off-chip remote valve switching and fluid flow rate changes are controlled using on-chip flow-rate changing fluid reservoirs. The present invention provides methods and systems for directional fluid control and control of fluid flow rate in an integrated microfluidic device which enables processes with different flow rates to be performed on one device without the need of on-chip valves. | 03-29-2012 |
20120077261 | DEVICE FOR A MEMBRANE ASSAY - Disclosed is a simple device for a membrane assay using the lateral flow immunoassay method, whereby a subject to be detected can be detected at a high sensitivity, provided with, as a label drying pad, a substrate which has a higher tensile strength than glass fiber and can well release a label. The present invention provides a simple membrane assay device, comprising: a supporting board, a sample supply part, a label containing a labeling component which labels a subject to be detected, a development part formed with a detection part which includes a trapping reagent for detecting or quantifying the subject to be detected, and an absorption part, wherein a non-woven fabric which includes fibers having a fiber diameter of 0.05 to 10 μm is used in the labeling component part. | 03-29-2012 |
20120083028 | DEVICE FOR FECAL OCCULT BLOOD TEST - A devise for fecal occult blood test comprises a first and a second casings, a sampling portion extending from the first casing, a specimen processing portion, at least a testing portion and a packing cylindrical member. The packing cylindrical member is coupled between the first and second casings when unused and dismantled therefrom when used. After sampling via a specimen collecting portion of the sampling portion, the specimen collecting portion is drawn into the first casing to reduce the device size. The first and second casings are fixedly coupled together in a specimen processing position thereof to mix specimens with a liquid of the specimen processing portion into a testing liquid, and then in a specimen testing position thereof to disperse onto the testing portions for testing and directly display test results. The present invention advantages high accuracy, rather hygiene, significant size reduction, real-time testing-result display and convenient use. | 04-05-2012 |
20120088294 | METHOD FOR SINGLE NUCLEOTIDE POLYMORPHISM AND MUTATION DETECTION USING REAL TIME POLYMERASE CHAIN REACTION MICROARRAY - A method and apparatus for real-time, simultaneous, qualitative measurement of one or more single nucleotide polymorphisms in one or more target nucleic acids is provided. This method involves combining a polymerase chain reaction (PCR) technique with an evanescent wave technique. | 04-12-2012 |
20120094368 | SPLIT SPIN CENTRIFUGATION OF TEST ELEMENTS - High-throughput centrifugation of batches of samples is achieved by dividing a single centrifugation run of a batch of samples into two or more staggered, discrete spins with fewer samples and in which the availability of centrifuge slots for loading or unloading of samples and the subsequent delivery of experimental results can be enhanced. The methodology is particularly useful in situations where multiple samples need to be processed rapidly, for example, as part of a STAT blood typing program in urgent care facilities prior to blood transfusion. | 04-19-2012 |
20120100600 | APPARATUS FOR POLYNUCLEOTIDE DETECTION AND QUANTITATION - An apparatus for expression profiling analysis, subjecting biological materials to polynucleotide extraction, amplification and analysis. The apparatus include an amplification device which permits the amplification of polynucleotides and an analysis device which quantifies the amount of the amplified polynucleotide products. The amplification device of the apparatus may further permit polynucleotide extraction to prepare the template for amplification, or sequence identification of a quantified polynucleotide product. A fraction collector may be included in the apparatus to collect a qualified polynucleotide product before its sequence is identified. The analysis device may further permit data generation, or alternatively, data can be generated by a separate data generation device provided with the apparatus. The devices within the apparatus are connected by connecting means which permit the transfer of a fluid or a signal for amplification and analysis. | 04-26-2012 |
20120115212 | Assay Tube - A non-instrumented biochemical assay device, such as a device for the processing of clinical specimens for molecular diagnostic applications. In one embodiment, an assay device comprises a lid which covers a reagent tube, which is disposed on a base. The platform utilizes chemical heating and optional temperature modulation by phase change material to prepare the sample and to amplify the target nucleic acid using Loop-Mediated Isothermal Amplification. The device features a simple indicator for a positive reaction, for example turbidity or fluorescence. | 05-10-2012 |
20120115213 | SYSTEMS FOR BIOAGENT IDENTIFICATION - The present invention provides systems and methods for analysis of samples, particularly biological and environmental sample to detect biomolecules of interest contained therein. A variety of system components are described herein, including, but not limited to, components for sample handling, mixing of materials, sample processing, transfer of materials, and analysis of materials. The invention further provides mechanisms for combining and integrating the different components and for housing, moving, and storing system components or the system as a whole. The systems may include any one or more or all of these components. The system finds particular use when employed for analysis of nucleic acid molecule using mass spectrometry, however, the invention is not limited such specific uses. | 05-10-2012 |
20120115214 | PORTABLE HIGH GAIN FLUORESCENCE DETECTION SYSTEM - An instrument for fluorometric assays in liquid samples is disclosed. The instrument may include multiple optical channels for monitoring a first fluorophore associated with a target analyte and a second fluorophore associated with a control. The disclosed instrument finds utility in any number of applications, including microfluidic molecular biological assays based on PCR amplification of target nucleic acids and fluorometric assays in general. | 05-10-2012 |
20120135510 | Electrokinetic Device for Capturing Assayable Agents in a Dielectric Fluid - Electrokinetic devices and methods are described with the purpose of collecting assayable agents from a dielectric fluid medium. Electrokinetic flow may be induced by the use of plasma generation at high voltage electrodes and consequent transport of charged particles in an electric voltage gradient. In one embodiment, the agents are directed electrokinetically to the sample collection assay device with no intermediate transfer steps. The agents are directed by creation of an electrokinetic potential well, which will effect their capture on to an assay device. Environmental agents such as biowarfare agents, pathogens, allergens or pollutants are collected autonomously on to the assay device, without any human intervention. The dielectric fluid medium, such as air, is sampled by electrokinetic propulsion with no moving parts or optionally, by transporting the dielectric fluid by a fan, pump or by breath. A further embodiment for collection of pathogen samples entails breathing into a tube where the sample is exposed to an electric plasma in the neighborhood of a high voltage electrode or electrodes, further transported by the breath through a potential well created at a sample collection device, where charge particles are electroprecipitated. The dielectric fluid medium may further include non-conductive liquids, such as oils. Oils may be sampled for the presence of contaminants, contaminating organisms or bio-degrading organisms. | 05-31-2012 |
20120135511 | PORTABLE HIGH GAIN FLUORESCENCE DETECTION SYSTEM - Disclosed is a compact, microprocessor-controlled instrument for fluorometric assays in liquid samples, the instrument having a floating stage with docking bay for receiving a microfluidic cartridge and a scanning detector head with on-board embedded microprocessor for controlling source LEDs, emission signal amplification and filtering in an isolated, low noise, high gain environment within the detector head. Multiple optical channels may be incorporated in the scanning head. In a preferred configuration, the assay is validated using dual channel optics for monitoring a first fluorophore associated with a target analyte and a second fluorophore associated with a control. Applications include molecular biological assays based on PCR amplification of target nucleic acids and fluorometric assays in general, many of which require temperature control during detection. Sensitivity and resistance to bubble interference during scanning are shown to be improved by use of a heating block with reflective mirror face in intimate contact with a thermo-optical window enclosing the liquid sample. | 05-31-2012 |
20120142085 | SYSTEMS FOR RAPID IDENTIFICATION OF PATHOGENS IN HUMANS AND ANIMALS - The present invention provides methods of: identifying pathogens in biological samples from humans and animals, resolving a plurality of etiologic agents present in samples obtained from humans and animals, determining detailed genetic information about such pathogens or etiologic agents, and rapid detection and identification of bioagents from environmental, clinical or other samples. | 06-07-2012 |
20120156766 | SAMPLE ANALYZING CHIP AND MEASUREMENT SYSTEM USING SAME - Temperature of a sensor chip itself rises with the supply of power owing to temperature dependence of the sensor chip as its basic characteristics. When a chemiluminescence reagent is added at the point of time at which the temperature rise reaches a steady state and a sensor chip photodiode dark current becomes constant, a drastic shift occurs in the sensor chip temperature. Remarkable dispersion occurs at this time in the sensor chip photodiode dark current Variance (unstability) of the sensor chip photodiode dark current can be decreased by reducing the temperature fluctuation of the sensor chip to minimum by using an exothermal effect of a thermal diffusion medium. | 06-21-2012 |
20120164717 | Identity profiling of cell surface markers - An apparatus and method of characterizing cells having surface markers includes the use of functionalized probes, the probes having absorption spectra characteristic of a probe geometry. The probes are functionalized with biomolecules (biomarkers) capable of binding to the surface markers, and cells having a particular combination of probes having specific bound biomarkers are measured using a spectrum analyzer. A plurality of surface markers may be simultaneously measured using the spectral properties of the probes to differentiate the cells. The relative abundance of a plurality of surface markers may be determined simultaneously. Functionalized probes with several different aspect ratios were used in an experiment to demonstrate the use of functionalized probes for characterizing cancer cells. The functionalized gold nanorod probes when attached to magnetic particles may be used as a dual contrast agent for magnetic resonance imaging (MRI) and optical imaging and as a targeted drug delivery system. | 06-28-2012 |
20120171758 | Device for extracting nucleic acid from a sample - The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample. | 07-05-2012 |
20120171759 | INTEGRATED APPARATUS FOR PERFORMING NUCLEIC ACID EXTRACTION AND DIAGNOSTIC TESTING ON MULTIPLE BIOLOGICAL SAMPLES - The technology described herein generally relates to systems for extracting polynucleotides from multiple samples, particularly from biological samples, and additionally to systems that subsequently amplify and detect the extracted polynucleotides. The technology more particularly relates to microfluidic systems that carry out PCR on multiple samples of nucleotides of interest within microfluidic channels, and detect those nucleotides. | 07-05-2012 |
20120184025 | NUCLEIC ACID ANALYZER - A nucleic acid analyzer includes a nucleic acid purification kit yielding a nucleic acid solution by isolating and purifying nucleic acid from a specimen; a nucleic acid analysis chip having a rotation axis positioned at a center thereof, with reaction containers at an outer portion than the rotation axis, and feeding the acid to the containers through centrifugal force; a specimen-introducing part on which the kit is placed; an analysis chip holder at the specimen-introducing part and supporting the chip; a purification treatment unit injecting the nucleic acid solution containing the nucleic acid into the chip; a centrifugal liquid feed unit feeding the nucleic acid solution to the containers by rotating the chip about the rotation axis; an analysis unit analyzing reaction products in the containers; and a transport part transporting the specimen-introducing part to the purification treatment unit, the centrifugal liquid feed unit, and the analysis unit. | 07-19-2012 |
20120202276 | Modified Proteins and Methods of Making and Using Same - Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions. | 08-09-2012 |
20120208263 | DEVICE FOR MONITORING A PLURALITY OF DISCRETE FLUORESCENCE SIGNALS - The present invention relates to a device and detector for monitoring a plurality of discrete fluorescence signals, in particular for DNA sequencing by use of fluorescently labeled nucleotides. The particular detector ( | 08-16-2012 |
20120214224 | Flow based clinical analysis - Values of clinical properties are normally measured by taking a sample from a patient, mixing an aliquot with a reagent, placing the mixture into a selected instrument, and measuring a property. If another property is required, another measurement sequence must be created. This can be efficient on a large scale, for example in a centralized laboratory, but is inefficient on a small scale. It is shown that by using measurement systems based on manipulation of flowing streams, clinical assays can be performed by a hand held device. This flow based system allows complex assays to be performed in remote locations with automated portable instruments that can be flexible enough to conduct a wide variety of assays. | 08-23-2012 |
20120220023 | ANALYSIS DEVICE WITH TRANSDUCER STIFFENING ELEMENTS - Disclosed herein is an analysis device for use with an external testing apparatus to detect the presence of an analyte in a test sample. The device comprises a transducer formed of a layer of pyroelectric or piezoelectric material sandwiched between first and second electrode layers, the transducer being arranged to produce an electrical voltage across the electrode layers in response to heating or straining of the pyroelectric or piezoelectric material layer. The device also comprises first and second stiffening elements for the transducer, the transducer being sandwiched between the stiffening elements. Each of the stiffening elements defines a planar surface for maintaining the transducer in a flat condition. Each of the stiffening elements exposes a portion of a respective electrode layer of the transducer for electrically connecting the transducer to the external text apparatus. The exposed portions are laterally offset from each other such that the exposed portions are each supported across the whole of their area by the stiffening element on the opposite side of the transducer. It has been found that such an arrangement may reduce noise caused by environmental effects. | 08-30-2012 |
20120231531 | BIOCHEMICAL DETECTION UNIT AND BIOCHEMICAL DEVICE HAVING THE SAME - A biochemical detection unit for detecting a sample and a biochemical device having the biochemical detection unit and a releasing unit are provided. The biochemical detection unit includes a photoconductor plate, a receptor, and a resistance sensing component. The receptor specifically binds to the sample so that the illumination projected on the photoconductor plate will change to vary the resistance value of the photoconductor of the photoconductor plate. | 09-13-2012 |
20120231532 | OPTICAL SCANNING SYSTEM - An optical scanning system including a switchable light source, a detector, a substrate and a plurality of optical sensing sites, as well as methods and kits for use thereof are provided. The substrate is coupled to and in optical communication with the switchable light source and the detector. Additionally, the substrate includes a plurality of substantially parallel excitation waveguides, and a plurality of substantially parallel collection waveguides, the excitation waveguides and collection waveguides crossing to form a two-dimensional array of intersection regions where an excitation waveguide and a collection waveguide cross and provide optical communication with the intersection region at each crossing. The plurality of optical sensing sites are each in optical communication with an intersection region. | 09-13-2012 |
20120244607 | Disc-Like Assay Chip - The present invention provides a disc-like assay chip, and more specifically, the present invention provides an assay chip which can effectively clean a cleaned object existing in a fluid loop, and can be appropriately applied to a detection method of a reaction system using a step of necessarily cleaning the cleaned object, such as an immunofluorescent antibody method, and applied to a detection method using an enzymatic reaction (especially, an ELISA method). The present invention further provides a disc-like assay chip which is mounted on a centrifugal device such as a rotational disc and can perform detection or quantitation on a target substance through optical measurement after a specimen and a reagent react by use of a centrifugal force generated by rotation of the centrifugal device. | 09-27-2012 |
20120252109 | REAGENT CARTRIDGE FOR AN ASSEMBLY FOR SELECTIVELY PERFORMING A CLINCIAL CHEMICAL TEST OR AN ELISA TEST, USE OF SAID REAGENT CARTRIDGE AND ASSEMBLY - The invention relates to a reagent cartridge | 10-04-2012 |
20120258524 | Annealing Curve Analysis In PCR - The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye. | 10-11-2012 |
20120264202 | SYSTEM FOR PERFORMING POLYMERASE CHAIN REACTION NUCLEIC ACID AMPLIFICATION - A printed circuit structure containing a fluidic chamber configured to receive an aqueous solution containing a sample to be analyzed and fluorophore for polymerase chain reaction analysis. The printed circuit structure also contains a heating element that provides for temperature cycling of the fluidic chamber to support polymerase chain reaction analysis. | 10-18-2012 |
20120264203 | HEAT FLOW POLYMERASE CHAIN REACTION SYSTEMS AND METHODS - Methods and systems for polymerase chain reactions (PCR) that are capable of detecting amplified DNA during or after the PCR process. The methods and systems may utilize DSC or DTA analysis techniques. | 10-18-2012 |
20120270305 | SYSTEMS, METHODS, AND APPARATUSES TO IMAGE A SAMPLE FOR BIOLOGICAL OR CHEMICAL ANALYSIS - A fluidic device holder configured to orient a fluidic device. The device holder includes a support structure configured to receive a fluidic device. The support structure includes a base surface that faces in a direction along the Z-axis and is configured to have the fluidic device positioned thereon. The device holder also includes a plurality of reference surfaces facing in respective directions along an XY-plane. The device holder also includes an alignment assembly having an actuator and a movable locator arm that is operatively coupled to the actuator. The locator arm has an engagement end. The actuator moves the locator arm between retracted and biased positions to move the engagement end away from and toward the reference surfaces. The locator arm is configured to hold the fluidic device against the reference surfaces when the locator arm is in the biased position. | 10-25-2012 |
20120270306 | METHOD AND APPARATUS FOR RAPIDLY COUNTING AND IDENTIFYING BIOLOGICAL PARTICLES IN A FLOW STREAM - A method for increasing the throughput and/or the precision of a flow cytometer, or a hematology analyzer employing a flow cytometer, and for further reducing the complexity of such a cytometer or analyzer. The system and method includes utilizing the technique of laser rastering in combination with a lysis-free single-dilution method. | 10-25-2012 |
20120282681 | Rapid Identification of Organisms in Bodily Fluids - There is provided a device that retains a collected sample for on-demand testing of a small portion of the collected sample while the rest of the sample remains for optional additional analysis. The on-demand test provides relatively immediate information about aspects of the sample, e.g. presence of microbes, chemistry, nutritional condition, presence of contaminants. | 11-08-2012 |
20120282682 | MICRO-CHANNEL STRUCTURE METHOD AND APPARATUS - A method is provided of forming a micro-channel structure for use in a biosensing device. A master structure is provided having a first configuration of micro-channels with respective first fluid flow characteristics. One or more regions of material are deposited onto the master structure using a fluidjet process so as to modify the first configuration into a second configuration having respective second fluid flow characteristics, different from the first. Functional biosensing devices formed using the method are also described. | 11-08-2012 |
20120282683 | SAMPLE ANALYSIS DEVICE - Disclosed is a sample analysis device provided with: a first sample processing portion which is disposed in a first layer and performs some of a plurality of processes on a sample in a container; a second sample processing portion which is disposed in a second layer located above or under the first layer and performs at least some other processes among the plurality of processes on the sample in the container, the some of the plurality of processes having been performed on the sample; and a container transfer portion which transfers the container, which contains the sample on which the some of the processes have been performed, from the first layer to the second layer. | 11-08-2012 |
20120282684 | AUTOMATED ANALYZER FOR CLINICAL LABORATORY - A laboratory automation system that is capable of carrying out clinical chemistry assays, immunoassays, amplification of nucleic acid assays, and any combination of the foregoing, said laboratory automation system employing at least one of micro-well plates and deep multi-well plates as reaction vessels. The use of micro-well plates as reaction vessels enables the laboratory automation system to assume a variety of arrangements, i.e., the laboratory automation system can comprise a variety of functional modules that can be arranged in various ways. In order to effectively carry out immunoassays by means of micro-well plates, a technique known as inverse magnetic particle processing can be used to transfer the product(s) of immunoassays from one micro-well of a micro-well plate to another. | 11-08-2012 |
20120288923 | Instrument and Method for Automatically Heat-Sealing a Microplate - A heating device for heating a thermally fixable sealing cover disposed over the microplate adjacent the wells, a cooling device for actively cooling the microplate and a controller set up to control activity of the heating and cooling devices in a manner to heat the sealing cover so as to thermally fix it to the microplate and to actively cool the microplate so as to keep a temperature of the samples below a predefined temperature when heating the sealing cover. It further relates to a method for automatically sealing a microplate in which the thermally fusible sealing cover is disposed over the microplate, the sealing cover is heated to thermally fix it to the microplate and the microplate is actively cooled in a manner that a temperature of the liquid reaction mixtures is kept below a predefined temperature when heating the sealing cover. | 11-15-2012 |
20120288924 | CARTRIDGE FOR CONDUCTING A CHEMICAL REACTION - A cartridge for conducting a chemical reaction includes a body having at least one flow path formed therein. The cartridge also includes a reaction vessel extending from the body for holding a reaction mixture for chemical reaction and optical detection. The vessel comprises a rigid frame defining the side walls of a reaction chamber. The frame includes at least one channel connecting the flow path to the chamber. The vessel also includes flexible films or sheets attached to opposite sides of the rigid frame to form opposing major walls of the chamber. In addition, at least two of the side walls are optically transmissive and angularly offset from each to permit real-time optical detection of analyte in the reaction chamber. | 11-15-2012 |
20120288925 | OPTICAL TRAPPING PARTICLES, ANGULAR OPTICAL TRAP SYSTEMS, METHODS OF MAKING, AND METHODS OF USE - The present invention relates to an optical trapping particle including a birefringent crystalline particle having a body and a length extending between a first end and a second end, said particle comprising an optic axis perpendicular to the length of the body, wherein the length of the body is greater than the largest width dimension of the first or second ends. The present invention also relates to an optical trapping particle including an optically isotropic particle having a body and a length extending between a first end and a second end, said particle having an asymmetric cross-section, wherein the length of the body is from about 10 nanometers to about 10 micrometers and is greater than the largest width dimension of the first or second ends. Angular optical trap systems including the optical trapping particles, methods of making, and methods of use are also disclosed. | 11-15-2012 |
20120295341 | THERMAL TREATMENT APPARATUS AND FLUID TREATMENT METHOD WITH FLUIDIC DEVICE - A thermal treatment apparatus includes a fluidic device including at least one channel, a first temperature-changing unit that changes the temperature of a fluid in part of the channel, and a second temperature-changing unit that changes the temperature of the fluid in another part of the channel. The temperature changes by the first and second temperature-changing units cause at least any one of the expansion and contraction of the fluid in the respective parts of the channel, and the at least any one of the expansion and contraction of the fluid due to the first temperature-changing unit is offset by the at least any one of the expansion and contraction of the fluid due to the second temperature-changing unit. | 11-22-2012 |
20120309079 | DETECTION OF EARLY STAGES AND LATE STAGES HPV INFECTION - Embodiments of the invention provide methods, monoclonal antibodies, polyclonal antibodies, assays, and kits for detecting HPV infection and HPV related cancer diagnosis, including infection by various HPV genotypes, early and/or late stage HPV-associated or HPV-specific cancers. Various specific or pan monoclonal antibodies recognizing specific epitope for specific HPV protein or HPV type, or common epitope for various HPV proteins or HPV types are obtained. The invention also provides one or more solid surface to coat the testing cell lysate. Also, the anti-HPV antibody can be coated on the solid surface of the invention to capture HPV proteins and detect HPV infection. | 12-06-2012 |
20120322139 | ANALYSIS DEVICE - Disclosed is an analysis device which can analyze substances with low blood concentration with high precision without having to make the device larger. The analysis device is composed of: a specimen disk equipped with specimen containers; a reagent disk equipped with reagent containers; a first disk equipped with first containers where purification of the subject component to be measured in the specimen is carried out; a second disk equipped with second containers where purification of the sample purified in the first container is carried out; and a mass spectrometry unit which measures the specimen purified in the second container. | 12-20-2012 |
20120329142 | INTEGRATED NUCLEIC ACID ASSAYS - Integrated microfluidic cartridges for nucleic acid extraction, amplification, and detection from clinical samples are disclosed. The devices are single-entry, sanitary, and disposable. The devices enable simplex or multiplex nucleic acid target detection, as for example: assay panels for multiple infectious agents, or assay panels for cancerous cell types. Methods for use of microfluidic cartridges in a fully automated, pneumatically controlled apparatus are also disclosed. | 12-27-2012 |
20120329143 | Chilled Reagent Container and Nucleic Acid Analyzer - A chilled reagent container comprises a reagent vessel containing part for containing therein a plurality of reagent vessels, a container lid including a container lid hole through which the reagent vessels contained by the reagent vessel containing part are accessible, and | 12-27-2012 |
20130005026 | CERVICAL CANCER SCREENING BY MOLECULAR DETECTION OF HUMAN PAPILLOMAVIRUS-INDUCED NEOPLASIA - Point-of-care tools for screening biological samples for markers associated with pathogenic microbial infections. In particular, devices and systems for screening cervical cells for the expression of proteins, which occur as a result of human papillomavirus infection and progression to invasive cervical cancer. | 01-03-2013 |
20130011912 | PORTABLE HIGH GAIN FLUORESCENCE DETECTION SYSTEM - An instrument for fluorometric assays in liquid samples is disclosed. The instrument may include multiple optical channels for monitoring a first fluorophore associated with a target analyte and a second fluorophore associated with a control. The disclosed instrument finds utility in any number of applications, including microfluidic molecular biological assays based on PCR amplification of target nucleic acids and fluorometric assays in general. | 01-10-2013 |
20130011913 | IMMUNOASSAYS, METHODS FOR CARRYING OUT IMMUNOASSAYS, IMMUNOASSAY KITS AND METHOD FOR MANUFACTURING IMMUNOASSAY KITS - The invention relates to immunoassays, methods for carrying out immunoassays, immunoassay kits and methods for manufacturing immunoassay kits. In particular, the invention has relevance to capillary (especially microcapillary) immunoassay technology. | 01-10-2013 |
20130023038 | Apparatus and Methods For Processing Biological Samples and a Reservoir Therefor - An apparatus for processing at least one biological sample accommodated on at least one carrier member ( | 01-24-2013 |
20130023039 | HIGH MULTIPLEX ARRAYS AND SYSTEMS - Apparatus, systems and methods for use in analyzing discrete reactions at ultra high multiplex with reduced optical noise, and increased system flexibility. Apparatus include substrates having integrated optical components that increase multiplex capability by one or more of increasing density of reaction regions, improving transmission of light to or collection of light from discrete reactions regions. Integrated optical components include reflective optical elements which re-direct illumination light and light emitted from the discrete regions to more efficiently collect emitted light. Particularly preferred applications include single molecule reaction analysis, such as polymerase mediated template dependent nucleic acid synthesis and sequence determination. | 01-24-2013 |
20130023040 | METHODS AND APPARATUS FOR SELECTIVELY PROCESSING EGGS HAVING IDENTIFIED CHARACTERISTICS - Methods and apparatus for processing eggs based upon a characteristic such as gender are provided. Material is extracted from each of a plurality of live eggs, the extracted material is assayed to identify eggs having the characteristic, and then eggs identified as having the characteristic are processed accordingly. | 01-24-2013 |
20130029407 | CELL ANALYZER - Provided is a cell concentration and purification device, having: a function of continuously concentrating cells; a function of then subsequently disposing the cells continuously in a specific region of a channel; a function of simultaneously recognizing, based on an image, the shape and fluorescence emission of each single cell; and a function of recognizing the cells and then separating and purifying the same based on the data relating to the shape and fluorescence emission thereof. | 01-31-2013 |
20130029408 | INTEGRATED SYSTEM FOR SAMPLING AND ANALYSIS - There is disclosed a device for determination of a sample from a dielectric fluid medium for a bio-specific assay device, comprising: a sampling device and a biosensor, wherein the sampling device concentrates a sample from dielectric medium by electrically focusing the sample on to a capture element and wherein said biosensor is fluidically linked to said capture element thus providing sampling and determination in a unitary device. | 01-31-2013 |
20130029409 | METHOD AND APPARATUS FOR AUTOMATIC STAINING OF TISSUE SAMPLES - The present invention concerns an apparatus for staining tissue samples, said apparatus including a reagent section or reagent containers; at least one staining section or tissue samples, a robotic head or robotic element that may move reagent to a predetermined tissue sample, said robotic element being moveable above the reagent and the staining sections, a control element that may manage a staining process, a 2-D optical sensor to detect two-dimensional image data of a relevant property and that can feed the captured image data to the control element. By providing the robotic element with a 2-D optical sensor, a common image processor may be provided having multiple functions. By using a 2-D optical image processing system, the control system of the apparatus may easily be adapted to read various types of data presentations, just as actual images for sections of the apparatus may be identified in order to assess the condition of the apparatus. The optical sensor may be used to automatically identify the slides and the reagent containers present in the apparatus, just as the optical sensor may be used for checking if a slide is misplaced at or absent from a slide position, etc. | 01-31-2013 |
20130040375 | ASSAY SYSTEMS FOR GENETIC ANALYSIS - The present invention provides assay systems and methods for detection of copy number variation at one or more loci and polymorphism detection at one or more loci in a mixed sample from an individual. | 02-14-2013 |
20130040376 | APPARATUS FOR AND METHOD OF PROCESSING BIOLOGICAL SAMPLES - The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like. | 02-14-2013 |
20130040377 | PCR DEVICE INCLUDING TWO HEATING BLOCKS - According to the present invention, a PCR device including two heating blocks which is used for nucleic acid amplification reactions is disclosed. Using the PCR device of the present invention, nucleic acid amplification reactions can be efficiently performed. | 02-14-2013 |
20130040378 | IMMUNOASSAY CUVETTES - The present invention is directed immunoassay cuvettes that comprise diffusely bound and non-diffusely bound reagents for carrying out an immunoassay. The reaction and detection are carried out in the immunoassay cuvette. The immunoassay cuvette comprises a transparent front wall, a back wall, side walls, a bottom, and a top opening. The back wall of the cuvette has a substantially planar surface made of a non-porous material and comprises a capture zone having reagents non-diffusedly bound and a signal reagent zone having reagents diffusedly bound. | 02-14-2013 |
20130052723 | LENS ASSEMBLY FOR BIOLOGICAL TESTING - The invention relates to an optical detection system for a thermal cycling device including at least one light source, a light detection device for detecting light received from a plurality of biological samples, and a lens having first and second surfaces formed on the lens, the second surface substantially opposed to the first surface. The first surface may be configured to collimate light and the second surface may be configured to direct light into each of the plurality of biological samples. | 02-28-2013 |
20130059366 | Integrated Droplet Actuator for Gel; Electrophoresis and Molecular Analysis - The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator. | 03-07-2013 |
20130059367 | ULTRASENSITIVE BIOSENSORS - The present invention is a biosensor apparatus that includes a substrate, a source on one side of the substrate, a drain spaced from the source, a conducting channel between the source and the drain, an insulator region, and receptors on a gate region for receiving target material. The receptors are contacted for changing current flow between the source and the drain. The source and the drain are relatively wide compared to length between the source and the drain through the conducting channel. | 03-07-2013 |
20130071915 | MICROWELL STRUCTURES FOR CHEMICALLY-SENSITIVE SENSOR ARRAYS - Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions. | 03-21-2013 |
20130078711 | SYSTEMS AND METHODS FOR PROVIDING MICROFLUIDIC DEVICES - A microfluidic valve system is disclosed that includes a matrix, a hydrophilic acceptor region a hydrophilic transfer region, and a hydrophobic gap between the acceptor region and the transfer region. | 03-28-2013 |
20130084628 | MICROWAVE ACCELERATED ASSAYS - The present invention provides for increasing fluorescence detection in surface assay systems while increasing kinetics of a bioreaction therein by providing low-power microwaves to irradiate metallic materials within the system in an amount sufficient to increase heat thereby affecting the kinetics of a bioreaction therein. | 04-04-2013 |
20130084629 | Nucleic Acid Analysis Reaction Cell and Nucleic Acid Analyzer - A nucleic acid analysis reaction cell and a nucleic acid analyzer are provided, in which a uniform flow rate is realised, so that a portion where a flow rate is low is removed and washing time for reagent removal is shortened. | 04-04-2013 |
20130089920 | MICROFLUIDIC FLOW CELL - A microfluidic flow cell having a body with a fluid transport channel disposed therein, the fluid transport channel having a proximal end and a distal end defining a fluid flow path, a fluid inlet port disposed at the proximal end of the fluid transport channel at a central portion of the body and an outlet port disposed at the distal end of the fluid transport channel at an outer portion of the body, and a plurality sample wells disposed in the fluid transport channel substantially perpendicular to the fluid flow path in the fluid transport channel. The microfluidic flow cell may have hundreds or thousands of individual, sub-microliter sample wells. The microfluidic flow cell can be filled by applying a flowable liquid to the inlet port and spinning the flow cell to cause fluid to flow into fluid transport channel. | 04-11-2013 |
20130089921 | DEVICE FOR DETECTING PROPERTIES OF CHEMICAL AND/OR BIOLOGICAL FLUIDS - The present invention relates to a device for investigating, measuring and/or detecting properties of chemical and/or biological fluids as well as to a device for performing these procedures and a system comprising a holder for holding at least one of said devices. A device for detecting properties of chemical and/or biological fluids may comprise a tubular structure with a cavity for accommodating the fluid, the tubular structure comprising a magnetizable and/or magnetic material, wherein the tubular structure comprises a segment that is transparent to light of wavelength 200 nm to 2000 nm. | 04-11-2013 |
20130102063 | SMALL MOLECULES AND PROTEIN ANALYSIS DEVICES BASED ON MOLECULAR IMPRINTED POLYMERS - Devices, methods and kits for rapid and simple determination of target molecules, including small molecules, polypeptides, proteins, cells and infectious disease agents in liquid samples that are capable of real-time measurement of these entities in fluid samples that are highly selective, highly sensitive, simple to operate, low cost, and portable. The devices, methods and kits also provide, in at least some embodiments, the use of MIPs in a flow through or lateral flow device. | 04-25-2013 |
20130102064 | RT-PCR CHIP WITH OPTICAL DETECTION - An apparatus ( | 04-25-2013 |
20130109082 | DEVICE FOR SEQUENTIALLY DISPENSING LIQUID REAGENTS TO A REACTION CHAMBER | 05-02-2013 |
20130115685 | POINT-OF-CARE FLUIDIC SYSTEMS AND USES THEREOF - This invention is in the field of medical devices. Specifically, the present invention provides portable medical devices that allow real-time detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications. | 05-09-2013 |
20130115686 | METHOD OF MANUFACTURING MICRO CHAMBER PLATE WITH BUILT-IN SAMPLE AND ANALYTIC MICRO CHAMBER PLATE, ANALYTIC MICRO CHAMBER PLATE AND APPARATUS SET FOR MANUFACTURING ANALYTIC MICRO CHAMBER PLATE WITH BUILT-IN SAMPLE - The present invention relates to a micro chamber plate, and more particularly, to an analytic micro chamber plate in which a plurality of reaction solutions including a primer or probe selectively reacting with a nucleic acid react with each other without cross-contamination to measure and analyze a fluorescence level in real-time so as to analyze biological sample solution including a large amount of nucleic acids. Also, the present invention relates to a method of manufacturing the analytic chamber plate. Also, the present invention relates to a method of manufacturing a micro chamber plate with a built-in sample used for manufacturing the analytic chamber plate. Also, the present invention relates to an apparatus set for manufacturing the micro chamber plate with a built-in sample. | 05-09-2013 |
20130122576 | DEVICE FOR AUTOMATICALLY ANALYZING NUCLEIC ACID - An apparatus for automatically analyzing a nucleic acid includes: a sample preprocessing device including a plurality of chambers in which reagents mixed with a sample are accommodated according to sample preprocessing process order for extracting a nucleic acid from the sample; and a nucleic amplifying and detecting device connected with the sample preprocessing device to receive the nucleic acid extracted from the sample. | 05-16-2013 |
20130122577 | KIT INCLUDING SEQUENCE SPECIFIC BINDING PROTEIN AND METHOD AND DEVICE FOR DETERMINING NUCLEOTIDE SEQUENCE OF TARGET NUCLEIC ACID - Provided are kits for determining a nucleotide sequence of a target nucleic acid, the kit including at least one sequence specific binding protein and a detectable tag. In accordance with a kit for determining a nucleotide sequence of a target nucleic acid according to one exemplary embodiment and a method and device for determining a nucleotide sequence of a target nucleic acid, the nucleotide sequence of the target nucleic acid may be more efficiently determined. | 05-16-2013 |
20130130363 | SUBSTRATES, SYSTEMS AND METHODS FOR ANALYZING MATERIALS - Substrates, systems and methods for analyzing materials that include waveguide arrays disposed upon or within the substrate such that evanescent fields emanating from the waveguides illuminate materials disposed upon or proximal to the surface of the substrate, permitting analysis of such materials. The substrates, systems and methods are used in a variety of analytical operations, including, inter alia, nucleic acid analysis, including hybridization and sequencing analyses, cellular analyses and other molecular analyses. | 05-23-2013 |
20130130364 | MICRODEVICE FOR PATHOGEN DETECTION - There is provided a microdevice for biomaterial detection, including a passive micromixer to mix a biomaterial, a first probe, and a second probe; a magnetic separation chamber connected with the passive micromixer; and a capillary electrophoresis channel connected with the magnetic separation chamber. | 05-23-2013 |
20130130365 | DEVICES - A method of analysis, instrument for analysis and device for use in such an instrument are provided, which perform a number of processes need to reach a useful result in the context of a wide variety of samples. The sequence of those processes being optimised. A device, instrument using the device and method of use are also provided which offer reliable performance of a heating based process, with minimal condensation and/or sample loss issues. | 05-23-2013 |
20130130366 | IN SITU HEAT INDUCED ANTIGEN RECOVERY AND STAINING APPARATUS AND METHOD - An automated in situ heat induced antigen recovery and staining method and apparatus for treating a plurality of microscope slides. The process of heat induced antigen recovery and the process of staining the biological sample on the microscope slide are conducted in the same apparatus, wherein the microscope slides do not need to be physically removed from one apparatus to another. Each treatment step occurs within the same reaction compartment. The reaction conditions of each reaction compartment for treating a slide can preferably be controlled independently, including the individualized application of reagents to each slide and the individualized treatment of each slide. | 05-23-2013 |
20130130367 | Method And Device For Combined Detection Of Viral And Bacterial Infections - A lateral flow assay detects and differentiates between viral and bacterial infections. A combined point of care diagnostic device tests markers for viral infection and markers for bacterial infection, to effectively assist in the rapid differentiation of viral and bacterial infections. In one preferred embodiment, the bacterial marker is CRP. In another preferred embodiment, the viral marker is MxA. In some embodiments, it is unnecessary to lyse the cells in the sample prior to applying it to the device. | 05-23-2013 |
20130137165 | GENE ANALYSIS DEVICE - A gene analysis device including an illumination optical system radiating exciting light onto a sample solution; a microfluidic device for forming a bio-reaction space of the sample solution and including at least one micro-chamber in which a reflection pattern is formed; and a detection optical system for detecting a fluorescence signal generated by a bio-reaction in the micro-chamber and comprising a photodetector. | 05-30-2013 |
20130137166 | SYSTEM AND METHOD INCLUDING ANALYTICAL UNITS - Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples. | 05-30-2013 |
20130143308 | SYSTEMS AND METHODS FOR FLUORESCENCE DETECTION WITH A MOVABLE DETECTION MODULE - A fluorescence detection apparatus for analyzing samples located in a plurality of wells in a thermal cycler and methods of use are provided. In one embodiment, the apparatus includes a support structure attachable to the thermal cycler and a detection module movably mountable on the support structure. The detection module includes one or more channels, each having an excitation light generator and an emission light detector both disposed within the detection module. When the support structure is attached to the thermal cycler and the detection module is mounted on the support structure, the detection module is movable so as to be positioned in optical communication with different ones of the plurality of wells. The detection module is removable from the support structure to allow easy replacement. | 06-06-2013 |
20130143309 | DIAGNOSTIC INSTRUMENT AND FLOW PROCESS - A diagnostic instrument having a cellular analysis system capable of running standardized immune monitoring panels. The system could include an automated and integrated specimen sampling method through a continuous flow process. The instrument could include a probe washer station, scheduler, cassette autoloader, bar coding system, and/or containment area common interface. An improved optimization test is proposed for instrument and flow cytometer quality assurance. The proposed method analyzes population separation for measuring instrument performance and/or sample quality. Such a method may also use population separation for measuring sample and/or run quality. | 06-06-2013 |
20130143310 | OPTICAL INSTRUMENT COMPRISING MULTI-NOTCH BEAM SPLITTER - An instrument is provided that can monitor nucleic acid sequence amplification reactions, for example, PCR amplification of DNA and DNA fragments. The instrument includes a multi-notch filter disposed along one or both of an excitation beam path and an emission beam path. Methods are also provided for monitoring nucleic acid sequence amplifications using an instrument that includes a multi-notch filter disposed along a beam path. | 06-06-2013 |
20130143311 | Immunoassay Analyzer and Immunoassay Method - The present invention provides an immunoassay analyzer capable of discriminating between normal coloring due to a specific immunoreaction and abnormal coloring due to a cause other than the specific immunoreaction in a measurement region of a sample analysis tool. An immunoassay analyzer | 06-06-2013 |
20130149775 | DEVICE FOR RAPID DETECTION OF INFECTIOUS AGENTS - A portable system for real time detection of the presence of an infectious agent in a biological sample employs a reagent which detects the presence of a specific infectious agent in the sample, and emits a detectable signal when the reagent reacts with the sample and detects the presence of the infectious agent. A test cartridge has a reaction chamber for receiving the sample and the reagent. The reaction chamber has a predetermined internal geometry and at least one inner surface. Introducing the sample and the reagent into the test cartridge mixes the sample and the reagent. A testing unit receives the test cartridge, and includes a sensor for detecting an emitted detectable signal. The detection of the emitted detectable signal is indicative of the presence of the infectious agent in the sample. | 06-13-2013 |
20130157347 | Method of Analyzing Cardiovascular Disorders and Uses Thereof - Compositions, systems, and methods comprising circulating endothelial cells (CECs) are provided. The compositions described herein utilize isolated CECs, including compositions in a form that allows analysis of the CECs. The systems described herein utilize isolated CECs and an analytical tool or an output from an analytical tool. The methods described herein are related to the use of isolated CECs and analytical tools for providing information, to a health care provider or the CEC donor, that is relevant to the cardiovascular health of the CEC donor. Thus, the compositions, systems and methods described herein involve both a transformation (e.g., non-isolated CECs to isolated CECs, or isolated CECs to analyzed CECs) and a machine (e.g., isolation tools and analytical tools). | 06-20-2013 |
20130157348 | DEVICE FOR DETECTING NUCLEIC ACIDS - The invention relates to a device that allows a target nucleic acid to be detected in a homogeneous batch using two different detection formats. The device comprises at least two heatable sample blocks and a reaction cartridge which contains a base ( | 06-20-2013 |
20130157349 | Self-Contained Biological Analysis - Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include nucleic acid amplification and detection and immuno-PCR. | 06-20-2013 |
20130164829 | Specific Gene Polymorphisms in Breast Cancer Diagnosis, Prevention and Treatment - A method for detecting a predisposition to breast cancer in a subject is provided, The method comprises detecting in a biological sample from the subject one or more polymorphisms in the sequence of CD44 gene, The presence of one or more polymorphisms in the sequence of CD44 gene indicates that the subject has a predisposition for developing breast cancer. | 06-27-2013 |
20130177970 | METHODS AND COMPOSITIONS FOR DETECTING THE ACTIVATION STATE OF MULTIPLE PROTEINS IN SINGLE CELLS - The invention provides methods and compositions for simultaneously detecting the activation state of a plurality of proteins in single cells using flow cytometry. The invention further provides methods and compositions of screening for bioactive agents capable of coordinately modulating the activity of a plurality of proteins in single cells. The methods and compositions can be used to determine the protein activation profile of a cell for predicting or diagnosing a disease state, and for monitoring treatment of a disease state. | 07-11-2013 |
20130177971 | NON-CONTACT HEATING TYPE OF GENE AMPLIFICATION SYSTEM - Disclosed is a non-contact heating type gene amplification system. The non-contact heating type gene amplification system includes separate light sources for each step of a polymerase chain reaction in order to perform a high-speed temperature cycle by moving a rotation unit, in which a plurality of sample chambers are formed, to places at which the sample chambers are controlled at the temperatures corresponding to each step. | 07-11-2013 |
20130183750 | ULTRA-SENSITIVE PORTABLE CAPILLARY SENSOR - A portable, lightweight, rugged, easy-to-operate biosensor useful for rapidly detecting cells, viruses, antibodies, and other proteins. A capillary tube has a capture antibody immobilized on its interior surface. The specific capture antibody is selected based upon a desired target analyte to be detected. A sample potentially containing the target antigen is introduced into the capillary tube. Thereafter, a second antibody labeled with a fluorescent dye is introduced. Upon excitation by electromagnetic energy, typically supplied by a laser, the fluorescence of the sample is captured and analyzed. The apparatus is extremely compact and rugged making it ideal for field use. In addition, accurate results may be obtained by relatively unskilled operators directly from a self-contained readout. Optionally, an external device (e.g., a computer) may be connected to the apparatus via an optional interface. The analysis time provided by the biosensor system of the invention is shorter than has heretofore been possible. | 07-18-2013 |
20130189768 | APPARATUS FOR POLYNUCLEOTIDE DETECTION AND QUANTITATION - An apparatus for expression profiling analysis, subjecting biological materials to polynucleotide extraction, amplification and analysis. The apparatus include an amplification device which permits the amplification of polynucleotides and an analysis device which quantifies the amount of the amplified polynucleotide products. The amplification device of the apparatus may further permit polynucleotide extraction to prepare the template for amplification, or sequence identification of a quantified polynucleotide product. A fraction collector may be included in the apparatus to collect a qualified polynucleotide product before its sequence is identified. The analysis device may further permit data generation, or alternatively, data can be generated by a separate data generation device provided with the apparatus. The devices within the apparatus are connected by connecting means which permit the transfer of a fluid or a signal for amplification and analysis. | 07-25-2013 |
20130203158 | Monitoring Method and Apparatus - A device for remote management of patients suffering from heart failure and hypertension can measure one or more biomarker. The device aids in monitoring the efficacy and safety of treatment in such patients. | 08-08-2013 |
20130210124 | DETERMINATION OF CANCER PREDISPOSITION - Disclosed herein are systems and methods for using demethylation of genomic DNA for diagnosing, predicting, and/or monitoring the status or outcome of a neoplasm or a cancer in a subject. | 08-15-2013 |
20130210125 | MICROFLUIDIC CARTRIDGE FOR PROCESSING AND DETECTING NUCLEIC ACIDS - A microfluidic cartridge, configured to facilitate processing and detection of nucleic acids, comprising: a top layer comprising a set of cartridge-aligning indentations, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of Detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a Detection chamber, comprises a turnabout portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region. | 08-15-2013 |
20130210126 | MICROFLUIDIC CARTRIDGE FOR PROCESSING AND DETECTING NUCLEIC ACIDS - A microfluidic cartridge, configured to facilitate processing and detection of nucleic acids, comprising: a top layer comprising a set of cartridge-aligning indentations, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of Detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a Detection chamber, comprises a turnabout portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region. | 08-15-2013 |
20130210127 | SYSTEM AND METHOD FOR PROCESSING AND DETECTING NUCLEIC ACIDS - A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a capture plate and a capture plate module configured to facilitate binding of nucleic acids within the set of biological samples to magnetic beads; a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols; and an assay strip configured to combine nucleic acid samples with molecular diagnostic reagents for analysis of nucleic acids. | 08-15-2013 |
20130210128 | METHODS AND APPARATUS FOR MEASURING ANALYTES - Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions. | 08-15-2013 |
20130210129 | Unitary Biochip Providing Sample-in to Results-out Processing and Methods of Manufacture - A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided. | 08-15-2013 |
20130217103 | Droplet Operations Platform - The invention relates to a droplet actuator device and methods for integrated sample preparation and analysis of a biological sample. A droplet actuator device is provided for conducting droplet operations. The droplet actuator device may include a bottom substrate and a top substrate separated from each other to form a gap therebetween; an arrangement of droplet operations electrodes arranged on one or both of the bottom and/or top substrates for conducting droplet operations thereon; a reagent storage layer comprising one or more compartments bound to the top substrate; and one or more openings arranged to provide a fluidic path from the one or more compartments into the gap, upon breach of a breachable seal separating the one or more compartments and openings. | 08-22-2013 |
20130217104 | MICROFLUIDIC CHIP FEATURES FOR OPTICAL AND THERMAL ISOLATION - A microfluidic chip includes microfluidic channels, elements for thermally and optically isolating the microfluidic channels, and elements for enhancing the detection of optical signal emitted from the microfluidic channels. The thermal and optical isolation elements may comprise barrier channels interposed between adjacently-arranged pairs of microfluidic channels for preventing thermal and optical cross-talk between the adjacent microfluidic channels. The isolation element may alternatively comprise reflective film embedded in the microfluidic chip between the adjacent microfluidic channels. The signal enhancement elements comprise structures disposed adjacent to the microfluidic channels that reflect light passing through or emitted from the microfluidic channel in a direction toward a detector. The structures may comprise channels or a faceted surface that redirects the light by total internal reflection or reflective film material embedded in the microfluidic chip. | 08-22-2013 |
20130217105 | APPARATUS FOR MEASURING VOLUME OF A FLUID - An apparatus for transfer of a liquid using a diaphragm that separates a working fluid volume from a working air volume and can controllably induce a change pressure to draw or expel a target fluid into a tube. The apparatus is particularly suitable for automated processing of nucleic acids and other samples includes a disposable container comprising a tray and a flexible barrier. The barrier is configured to seal with a top edge of the tray, providing a closed, aseptic work area within the sealed tray. A pipette head and/or other sample manipulation device can be attached to the inside of the barrier under the diaphraghm, and the barrier can include an interface for a robotic arm or other device. When the barrier is sealed over the tray, the barrier separates the contents of the tray from the robot or other manipulation device. | 08-22-2013 |
20130217106 | BIOCHEMICAL ANALYSIS APPARATUS AND ROTARY VALVE - An analysis apparatus for performing biochemical analysis of a sample using nanopores comprises: a sensor device that supports plural nanopores, reservoirs holding material for performing the analysis; a fluidics system; and plural containers for receiving respective samples, all arranged in a cartridge that is removably attachable to an electronics unit arranged to generate drive signals to perform signal processing circuit to generate output data representing the results of the analysis. The fluidics system supplies samples selectively from the containers to the sensor device using a rotary valve. | 08-22-2013 |
20130224845 | FLUID INTERFACE CARTRIDGE FOR A MICROFLUIDIC CHIP - An interface cartridge for a microfluidic chip, with microfluidic process channels and fluidic connection holes at opposed ends of the process channels, provides ancillary fluid structure, including fluid flow channels and input and/or waste wells, which mix and/or convey reaction fluids to the fluidic connection holes and into the process channels of the microfluidic chip. | 08-29-2013 |
20130224846 | Universal Sample Preparation System and Use in an Integrated Analysis System - The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft | 08-29-2013 |
20130224847 | OPTICAL HEADS AND SEQUENCING APPARATUSES INCLUDING THE SAME - A sequencing apparatus may include a bio-disk having reaction regions in which nucleic acids are polymerized; a stage configured to revolve around an axis, the stage including optical heads for detecting lights emitted from the plurality of reaction regions due to the polymerization of the nucleic acids; and/or a control unit configured to generate data regarding base sequences of the nucleic acids in the reaction regions based on wavelengths of the lights detected by the plurality of optical heads. An optical head may include a light emitting unit configured to emit light toward a reaction region in which nucleic acids are polymerized; a light receiving unit configured to receive the light emitted by the light emitting unit; a de-multiplexing unit configured to de-multiplex the light received by the light receiving unit; and/or a plurality of photoelectric converting units configured to convert the de-multiplexed light to electrical signals. | 08-29-2013 |
20130224848 | Device and Method for Detection and Quantification of Immunological Proteins, Pathogenic and Microbial Agents and Cells - The present invention provides a method and device for detecting and quantifying the concentration of magnetic-responsive micro-beads dispersed in a liquid sample. Also provided is a method and microfluidic immunoassay pScreen™ device for detecting and quantifying the concentration of an analyte in a sample medium by using antigen-specific antibody-coated magnetic-responsive micro-beads. The methods and devices of the present invention have broad applications for point-of-care diagnostics by allowing quantification of a large variety of analytes, such as proteins, protein fragments, antigens, antibodies, antibody fragments, peptides, RNA, RNA fragments, functionalized magnetic micro-beads specific to CD | 08-29-2013 |
20130230908 | REACTION PLATE ASSEMBLY, REACTION PLATE AND NUCLEIC ACID ANALYSIS DEVICE - Provided is a technology such that, in nucleic acid analysis, a high degree of freedom in loading or unloading a reaction plate can be obtained and a sample can be efficiently analyzed. A reaction plate assembly includes a reaction plate with one or more reaction wells, a visible light transmissive cover mounted on the reaction plate and covering the reaction wells, and a visible light transmissive weight member covering the cover. The reaction wells are disposed in an arc shape along the circumference of a circle with a predetermined radius r | 09-05-2013 |
20130230909 | METHODS FOR ACCURATE SEQUENCE DATA AND MODIFIED BASE POSITION DETERMINATION - Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample. | 09-05-2013 |
20130244313 | SYSTEM AND DEVICE FOR ANALYZING A FLUIDIC SAMPLE - A device for analyzing an analyte in a sample includes a first substrate, a second substrate, a fluidic channel, an inlet port and an outlet port. Each of the first substrate and the second substrate has an inner surface and an outer surface, the inner surface of the first substrate forming, at least in part, the lower wall of the fluidic channel, and the inner surface of the second substrate forming, at least in part, the upper wall of the fluidic channel. The fluidic channel is connected to the inlet port and the outlet port. The fluidic channel includes a reagent region and a detection region, at least a portion of the reagent region being coated with one or more dried reagents. The device further includes a wicking pad located on the outer surface of the second substrate, the wicking pad being positioned at a pre-determined distance from the outlet port. | 09-19-2013 |
20130244314 | IMMUNOCHROMATOGRAPHIC TEST STRIP AND MANUFACTURING METHOD THEREOF - An immunochromatographic test strip that achieves a shorter reaction completion time and excellent sensitivity is provided. The immunochromatographic test strip comprises: (1) a conjugate pad comprising a sample-supplying section supplying a sample possibly containing an analyte and a line-shaped conjugate section containing a conjugate in which an antibody or antigen immunologically reactive with the analyte is immobilized to a label on the downstream side relative to the sample-supplying section; and (2) an insoluble membrane support having at least one detecting section to which an antibody or antigen immunologically reactive with the analyte is immobilized, the lower surface of the sample-supplying section of the conjugate pad being not in contact with the upper surface of the insoluble membrane support, the lower surface of the conjugate section of the conjugate pad being in contact with the upper surface of the insoluble membrane support. | 09-19-2013 |
20130244315 | ANALYTICAL DEVICES WITH INTEGRATED DESICCANT - An analytical device for performing an assay to determine the presence or approximate quantity of at least one analyte in a liquid sample is described. The device is manufactured to include an integrated desiccant within at least a test strip of the device. Addition of an integrated desiccant within the device improves signal to noise ratio, eases the manufacturing process, and saves in cost of production of the device. | 09-19-2013 |
20130252319 | BIOSENSOR FOR MEASURING STRESS BASED ON ELETRICAL DEVICE AND MEASUREMENT METHOD THEREOF, AND EMOTION-ON-A-CHIP AND MEASURING APPARATUS THEREOF - Biosensor and measurement method thereof, where cortisol in saliva is measured by immobilizing antibody for measuring the cortisol and reading an electrical signal generated when the antibody is bound to the cortisol using a miniaturized microwave resonant device, are provided. Emotion-on-a-chip and measurement apparatus thereof, where various emotion indexes are measured on the chip by measuring emotion index target material in real time from body fluid, are provided. Emotion diagnosis system is provided, which includes an emotion-on-a-chip where a biosensor detecting emotion signal including stress is mounted; and an emotion diagnosis apparatus converting the emotion signal from the emotion-on-a-chip into emotion level and outputting the emotion level, where the emotion-on-a-chip includes a ring resonator on dielectric layer; and a microstrip transmission line on the dielectric layer as straight signal line. | 09-26-2013 |
20130252320 | MODULAR POINT-OF-CARE DEVICES, SYSTEMS, AND USES THEREOF - The present invention provides devices and systems for use at the point of care. The methods devices of the invention are directed toward automatic detection of analytes in a bodily fluid. The components of the device are modular to allow for flexibility and robustness of use with the disclosed methods for a variety of medical applications. | 09-26-2013 |
20130252321 | STRUCTURES FOR CONTROLLING LIGHT INTERACTION WITH MICROFLUIDIC DEVICES - Systems and methods for improved measurement of absorbance/transmission through fluidic systems are described. Specifically, in one set of embodiments, optical elements are fabricated on one side of a transparent fluidic device opposite a series of fluidic channels. The optical elements may guide incident light passing through the device such that most of the light is dispersed away from specific areas of the device, such as intervening portions between the fluidic channels. By decreasing the amount of light incident upon these intervening portions, the amount of noise in the detection signal can be decreased when using certain optical detection systems. | 09-26-2013 |
20130260447 | SYSTEMS AND METHODS FOR HANDLING MICROFLUIDIC DROPLETS - The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel. | 10-03-2013 |
20130267015 | Fluidic Interfacing System and Assembly - A fluidic assay system assembly comprising: (a) a disposable fluidic cartridge ( | 10-10-2013 |
20130267016 | SYSTEM AND CARTRIDGE FOR EFFICIENT NUCLEIC ACID TESTING - A disposable and inexpensive biological diagnostic cartridge for the amplification and detection of nucleic acids includes a configuration having a reaction pouch for amplification which is compressed by a flexible pump pouch for detection of the amplified reaction. | 10-10-2013 |
20130273643 | METHODS AND APPARATUSES FOR PREDICTING RISK OF PROSTATE CANCER AND PROSTATE GLAND VOLUME - Methods and apparatuses for predicting risk of prostate cancer and/or prostate gland volume are provided. More particularly, this disclosure relates to methods and apparatuses for providing the models and employing the models for predicting risk of prostate cancer and/or predicting prostate gland volume. The methods and apparatuses for predicting risk of prostate cancer and/or prostate gland volume are provided using, at least in part, information from a panel of kallikrein markers. | 10-17-2013 |
20130273644 | NUCLEIC ACID AMPLIFICATION REACTION STATION FOR DISPOSABLE TEST DEVICES - An instrument for conducting nucleic acid amplification reactions in a disposable test device is provided. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber. | 10-17-2013 |
20130273645 | DETECTION INSTRUMENT, AND DETECTION SYSTEM - The present invention provides a detection instrument capable of easily detecting an intended detection object without any skilled technique. The detection instrument ( | 10-17-2013 |
20130280795 | SAMPLING AND TESTING DEVICE FOR THE HUMAN OR ANIMAL BODY - A test device comprises first and second indicia portions. The first indicia portion is configured to indicate the presence or absence of at least one medical condition based on identification of at least one biological entity in the biological sample. The second indicia portion is configured to display all or part of a code when the first indicia portion provides an indication of the presence of the medical condition, the code being substantially unique to the test device. The test device is employed in a medication dispensing system. | 10-24-2013 |
20130280796 | POROUS MEMBRANE WAVEGUIDE SENSORS AND SENSING SYSTEMS THEREFROM FOR DETECTING BIOLOGICAL OR CHEMICAL TARGETS - A sensor for sensing at least one biological target or chemical target is provided. The sensor includes a membrane includes a membrane material that supports generation and propagation of at least one waveguide mode, where the membrane material includes a plurality of voids having an average size <2 microns. The sensor also includes at least one receptor having structure for binding to the target within the plurality of voids, and an optical coupler for coupling light to the membrane sufficient to generate the waveguide mode in the membrane from photons incident on the optical coupler. | 10-24-2013 |
20130288347 | SCANNING ANALYZER FOR SINGLE MOLECULE DETECTION AND METHODS OF USE - The invention encompasses analyzers and analyzer systems that include a single molecule analyzer, methods of using the analyzer and analyzer systems to analyze samples, either for single molecules or for molecular complexes. The single molecule uses electromagnetic radiation that is translated through the sample to detect the presence or absence of a single molecule. The single molecule analyzer provided herein is useful for diagnostics because the analyzer detects single molecules with zero carryover between samples. | 10-31-2013 |
20130288348 | INSTRUMENTS FOR MIXING THE CONTENTS OF A DETECTION CHAMBER - A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle. | 10-31-2013 |
20130288349 | SPINNING FORCE APPARATUS - A spinning force system and methods of operation are provided for measuring a characteristic of a sample. The system includes a detection module having a light source for illuminating the sample and an objective being aligned to the light source to define a light path for producing an image of the sample. A rotor is mechanically coupled to the detection module and configured to rotate the light path for applying a force to the sample. The force may include a centrifugal force and other forms of force (such as a viscous drag force) resulted from the rotation. In some examples, the force is applied in a direction that is not parallel to a surface of the sample. | 10-31-2013 |
20130288350 | SENSOR - A sequencer that measures a nucleic acid sequence in a nucleic acid strand includes: a base material having a surface made of silicon, and a fibrous protrusion that is made of silicon dioxide and is directly joined to the surface of the base material made of silicon, wherein a plurality of the nucleic acid strands are fixed onto the fibrous protrusion. | 10-31-2013 |
20130288351 | CELL NUCLEUS OBSERVATION SUBSTRATE AND CELL NUCLEUS OBSERVATION APPARATUS - To provide a technology of staining nuclei of cells, microorganisms and the like by a simple operation and observing their forms. | 10-31-2013 |
20130295655 | MICRO-FLUIDIC DEVICE - Embodiments described herein provide micro-fluidic systems and devices for use in performing various diagnostic and analytical tests. According to one embodiment, the micro-fluidic device includes a sample chamber for receiving a sample, and a reaction chamber for performing a chemical reaction. A bubble jet pump is structured on the device to control delivery of a fluid from the sample chamber to the reaction chamber. The pump is fluidically coupled to one or more chambers of the device using a fluidic channel such as a capillary. A valve may be coupled to one or more chambers to control flow into and out of those chambers. Also, a sensor may be positioned in one or more of the chambers, such as the reactant chamber, for sensing a property of the fluid within the chamber as well as the presence of a chemical within the chamber. | 11-07-2013 |
20130295656 | Anaplasma Translocated Substrate-1 (Ats-1) and Sero-detection of Anaplasma phagocytophilum - Disclosed is the use of isolated Ats-1 protein in | 11-07-2013 |
20130295657 | Device and Method for the Detection of Particles - The present invention relates to devices and methods for the qualitative and/or quantitative detection of particles. In particular, the invention relates to devices for the detection of particles, comprising a reaction chamber formed within a chamber body between a first surface and a second surface, wherein the second surface is located opposite to the first surface, and one or more displacers, wherein the distance between the first surface and the second surface is variable via the one or more displacers at least in one or more parts of the surface area of the first surface and/or second surface. The invention also relates to corresponding methods for the detection of particles. | 11-07-2013 |
20130302883 | METHODS, SYSTEMS AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS - Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases. | 11-14-2013 |
20130302884 | METHODS, SYSTEMS AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS - Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases. | 11-14-2013 |
20130316440 | DEVICE FOR INSPECTING A BIOLOGICAL FLUID - A device for inspecting a biological fluid, including a channel through which the fluid flows, a first inspection module arranged in a first region of the channel, and a second inspection module arranged in a second region of the channel, the device configured to provide a quantity that is representative of output of the second inspection module. The first inspection module is configured to measure at least one electrical property of the fluid passing through the first region. The second inspection module is configured to measure at least one optical property of the fluid passing through the second region. The inspection device also includes a controller connected to the first inspection module and to the second inspection module and configured to control the second inspection module according to the output of the first inspection module. | 11-28-2013 |
20130316441 | GENETIC ANALYZER - A nucleic acid analysis apparatus capable of selecting an optimal analysis method for every user and improving throughput is provided. In a genetic analyzer for measuring and analyzing amplification reaction of a nucleic acid in real time, an amplification curve is analyzed and a user can select conditions for terminating the amplification reaction upon detection of amplification. Further, a user can select conditions for selecting next processing after termination of the amplification reaction. A user can select, in situ, conditions for terminating the amplification reaction and conditions for selecting next processing upon detection of amplification and after the termination of amplification reaction. Alternatively, conditions for terminating the amplification reaction and conditions for selecting the next processing are registered previously and processing is performed automatically upon detection of amplification and after termination of the amplification reaction. | 11-28-2013 |
20130323825 | INFORMATION PROCESSING APPARATUS, INFORMATION PROCESSING METHOD, AND PROGRAM - Provided is an information processing apparatus, including a testing section performing statistical testing on simple staining data obtained by performing fluorescence measurement on a particle subjected to simple staining with a staining material having a prescribed fluorescence characteristic and unstaining data obtained by performing fluorescence measurement on an unstained particle for comparison for a frequency band, a masking processing section setting, in a case where there is no significant difference between the simple staining data and the unstaining data for the frequency band, the simple staining data to 0 or a prescribed value, and an estimation section estimating, in a manner that double staining data obtained by performing fluorescence measurement on a particle stained with a plurality of staining materials is represented by a linear combination of base vectors representing a distribution of the simple staining data corresponding to each staining material, a combination coefficient of the linear combination. | 12-05-2013 |
20130323826 | AMPLIFICATION SYSTEM WITH SPATIAL SEPARATION - An automated nucleic acid analysis method and analytical system are described comprising separate modules, wherein the air flow of any one of said modules is controlled and wherein at least the air flow between the module for isolation and purification of the analyte and the module for analysis of the analyte are separated. | 12-05-2013 |
20130323827 | BIOCHIP INCLUDING CONDUCTIVE PARTICLE AND DEVICE FOR DETECTING TARGET ANTIGEN COMPRISING THE SAME - A biochip including conductive particle and a device for detecting target antigen comprising the biochip are disclosed. According to the present invention, a target antigen can be effectively detected using a small amount of target antigen alone, whereby nonspecific detection signal can be reduced and an amplified signal can be detected. | 12-05-2013 |
20130330812 | SENSOR FOR DETECTION OF A TARGET OF INTEREST - Embodiments of the present disclosure set forth a sensor for detecting a target of interest. One exemplary sensor may comprise a container; a probe disposed in the container and configured to bind to a target of interest; a circulation means configured to circulate substances in the container; a light source; a light receiver; a light selecting unit for allowing light of a predetermined wavelength be received by the light receiver; and a detector configured to generate an electrical signal. The magnitude of the electrical signal reflects the amount of light that is received by the light receiver. The container is located between the light source and the light receiver. Moreover, the container is configured such that the probe and the path of the light emitted by the light source are in different regions inside the container. | 12-12-2013 |
20130330813 | SENSOR FOR DETECTION OF A TARGET OF INTEREST - Embodiments of the present disclosure set forth a sensor for detecting a target of interest. One example sensor may comprise an apparatus for binding the target of interest and a draining unit for draining fluid from the apparatus. The apparatus may comprise a substrate, a material disposed on the substrate, and a probe disposed on the material and configured to bind to the target of interest. The probe is configured on the material to scatter light emitted from a light source when the target of interest is bound to the probe. | 12-12-2013 |
20130330814 | SENSOR FOR DETECTION OF A TARGET OF INTEREST - A sensor comprises a light source that emits light, a light receiver for receiving light, a sampling unit for binding the target of interest disposed between the light source and the light receiver, a light selecting unit for allowing light of a predetermined wavelength be received by the light receiver, and a detector configured to generate an electrical signal, and the magnitude of which reflects the amount of light that is received by the light receiver. The sampling unit comprises a substrate, a material disposed on the substrate, and a probe disposed on the material and configured to bind to the target of interest. The sampling unit is configured such that the probe is in the path of the light emitted by the light source. | 12-12-2013 |
20130344578 | METHODS AND MATERIALS FOR ASSESSING RNA EXPRESSION - This document provides methods and materials for assessing RNA expression. For example, methods and materials for detecting the presence, absence, or amount of target nucleic acid (e.g., target RNA or target cDNA produced from target RNA), kits for detecting the presence, absence, or amount of target nucleic acid (e.g., target RNA or target cDNA produced from target RNA), and methods for making such kits are provided. | 12-26-2013 |
20140017773 | MULTIPLE DISCRIMINATION DEVICE AND METHOD OF MANUFACTURING THE DEVICE - Provided are a multiple discrimination device and a method of manufacturing the same. According to the multiple discrimination device, a three-dimensional micro ferromagnetic pattern is optimally designed and arranged to allow a magnetic force applied to a discrimination-target particle to be discriminated to be well controlled to perform discrimination well. The method employs a semiconductor processing technology, thereby precisely manufacturing and allowing mass production. | 01-16-2014 |
20140017774 | PCA3, PCA3 GENES, AND METHODS OF USE - The present invention relates, in general, to a prostate-specific antigen, PCA3. In particular, the present invention relates to nucleic acid molecules coding for the PCA3 protein; purified PCA3 proteins and polypeptides; recombinant nucleic acid molecules; cells containing the recombinant nucleic acid molecules; antibodies having binding affinity specifically to PCA3 proteins and polypeptides; hybridomas containing the antibodies; nucleic acid probes for the detection of nucleic acids encoding PCA3 proteins; a method of detecting nucleic acids encoding PCA3 proteins or polypeptides in a sample; kits containing nucleic acid probes or antibodies; bioassays using the nucleic acid sequence, protein or antibodies of this invention to diagnose, assess, or prognose a mammal afflicted with prostate cancer; therapeutic uses; and methods of preventing prostate cancer in an animal. | 01-16-2014 |
20140030798 | PROCESSING POLYNUCLEOTIDE-CONTAINING SAMPLES - Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that regain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed. | 01-30-2014 |
20140030799 | APPARATUS FOR DETECTING TUMOR CELLS - Among others, the present invention provides apparatus for detecting circulating tumor cells, comprising a system delivery biological subject and a probing and detecting device, wherein the probing and detecting device includes a first micro-device and a first substrate supporting the first micro-device, the first micro-device contacts a biologic material to be detected and is capable of measuring at the microscopic level an electrical, magnetic, electromagnetic, thermal, optical, acoustical, biological, chemical, electro-mechanical, electro-chemical, electro-optical, electro-thermal, electro-chemical-mechanical, bio-chemical, bio-mechanical, bio-optical, bio-thermal, bio-physical, bio-electro-mechanical, bio-electro-chemical, bio-electro-optical, bio-electro-thermal, bio-mechanical-optical, bio-mechanical thermal, bio-thermal-optical, bio-electro-chemical-optical, bio-electro-mechanical-optical, bio-electro-thermal-optical, bio-electro-chemical-mechanical, physical or mechanical property, or a combination thereof, of the biologic subject. | 01-30-2014 |
20140038272 | THERMAL CYCLING SYSTEM AND METHOD OF USE - A temperature cycling system ( | 02-06-2014 |
20140038273 | Nano Biofuel Production Processes: Using Nantechnology to Enhance Produciton fo Biofuels - Methods, systems, devices and materials for producing biofuels under nanoscale control (“nanobiofuels”) are provided. In one aspect, the invention provides method for producing a biofuel, including providing a hydrocarbon producing organism; exposing the biological hydrocarbon producing organism to conditions effective to cause substantial release of the hydrocarbon from the biological hydrocarbon producing organism; and isolating at least a portion of the hydrocarbon. At least one of the actions of providing, exposing, and isolating is performed using a corresponding nanoscale control. | 02-06-2014 |
20140038274 | NUCLEIC ACID ANALYSIS DEVICE, NUCLEIC ACID ANALYSIS APPARATUS, AND NUCLEIC ACID ANALYSIS METHOD - The present invention relates to a nucleic acid analysis device in a nucleic acid analysis apparatus, whereby waste of reaction spots on the nucleic acid analysis device is eliminated and leakage of fluorescence excitation light to unobserved nucleic acid measurement regions is minimized. Specifically, the nucleic acid analysis device has a plurality of nucleic acid measurement regions, which are characterized in that one nucleic acid measurement region is disposed at a sufficient distance from the other nucleic acid measurement regions such that the other nucleic acid measurement regions do not enter an irradiation region. | 02-06-2014 |
20140045249 | CENTRIFUGAL PARTICLE SEPARATION AND DETECTION DEVICE - The present invention provides a centrifugal particle separation and detection device, which can separate and detect particles according to the particle size. The centrifugal particle separate and detection device is practiced with a centrifuge to separate particles, and can be applied to cancer cell detection, blood lymphocyte isolation, tissue engineering, polynucleotide hybridization, microorganism separation and detection and fine chemical purification. | 02-13-2014 |
20140045250 | REAL-TIME OPTICAL SYSTEM FOR POLYMERASE CHAIN REACTION - An improved device and system for facilitating polymerase chain reaction including a light source, detector, waveguide, and filters that occupy minimal space and facilitate reduced sample read time and rapid reading of multiple light wavelengths. | 02-13-2014 |
20140051154 | SYSTEMS AND METHODS TO DETECT COPY NUMBER VARIATION - In one aspect, a system for implementing a copy number variation analysis method, is disclosed. The system can include a nucleic acid sequencer and a computing device in communications with the nucleic acid sequencer. The nucleic acid sequencer can be configured to interrogate a sample to produce a nucleic acid sequence data file containing a plurality of nucleic acid sequence reads. In various embodiments, the computing device can be a workstation, mainframe computer, personal computer, mobile device, etc. | 02-20-2014 |
20140051155 | THERMAL CYCLER APPARATUS AND RELATED METHODS - An apparatus for thermal cycling can transfer heat uniformly and efficiently. The apparatus can be used in a method that reduces condensation on sample wells. The apparatus can also be manufactured to provide uniform configurations. For example, a sample, illustratively for polymerase chain reaction (PCR), in each sample well and the components of the embodiment of the thermal cycler apparatus shown at including a well block, a base plate, a layer of adhesive, a peltier device, another layer of adhesive and a heat sink | 02-20-2014 |
20140057339 | SYSTEMS AND METHODS FOR GENETIC AND BIOLOGICAL ANALYSIS - The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices. | 02-27-2014 |
20140057340 | DEVICES FOR DETECTING OR FILTERING TUMOR CELLS - Among others, the present invention provides devices each including a micro-filter, a shutter, a cell counter, a selector, a micro-surgical kit, a timer, and a data processing circuitry, wherein the micro-filter is capable of detecting or filtering circulating tumor cells. | 02-27-2014 |
20140073042 | FLUIDIC DEVICE, CHEMICAL REACTION SYSTEM, AND NUCLEIC-ACID ANALYZING SYSTEM - A fluidic device in which a first member and a second member are connected by an expanding member is provided. The fluidic device includes a first member having a fluid channel communicating with an opening; and a second member having a supply channel supplying a fluid to the fluid channel through the opening. An expanding member having a through-hole is fit into the opening, the fluid channel and the supply channel communicate with each other through the through-hole in the expanding member, and the first member and the second member are connected to each other with at least the expanding member being in an expanded state. | 03-13-2014 |
20140080203 | Analytical Cartridge with Fluid Flow Control - Analytical cartridges, systems and methods of processing a sample for analysis using capillary flows. Vertical gradient sample filtration provides filtrate to an incubation chamber for a time controlled by a flow modulator at the outlet of the incubation chamber. The flow modulator can include a serpentine capillary flow path without side walls. Incubated filtrate can flow from the incubation chamber to a detection channel after a predetermined time. The detection chamber can include one or more analytical regions in a porous substrate for detection of two or more analytes on the same cartridge from the same sample. | 03-20-2014 |
20140080204 | Apparatus for the Rapid Diagnosis of Targets in Human Body Fluids - More particularly, the present invention relates to a method for the detection of a target, e.g. pathogen in a human body fluid wherein a body fluid sample is collected with a swab member. | 03-20-2014 |
20140087452 | IN-VITRO CONTACT LENS TESTING - Contact lens testing apparatuses and method for testing contact lenses for analytes are presented. In an aspect, a device is provided that includes a housing configured to hold one or more contact lenses, and a testing compartment provided within the housing and comprising a reagent, the reagent configured to facilitate a chemical reaction in response to the existence of a predetermined biomarker disposed on or within a contact lens placed in the testing compartment, wherein the chemical reaction produces a known result related to state information of an individual from which the biomarker was generated. | 03-27-2014 |
20140106441 | BIOAFFINITY SENSORS BASED ON SURFACE MONOLAYERS - Methods, systems, devices and materials are disclosed for implementing a bioaffinity sensor having a self-assembled monolayer interface for detection of a target molecule. In one aspect, a sensor device for detecting a target molecule includes a surface capable of attaching a thiol and a molecular monolayer formed on the surface that includes a molecular capture probe having a thiol region, a linear alkanethiol molecule having one thiol region, and a linear alkanedithiol molecule having two thiol regions, in which the molecular capture probe includes a region for receiving a target substance having a complimentary region that couples with the region of the molecular capture probe to generate a detectable signal. | 04-17-2014 |
20140106442 | NMR SYSTEMS AND METHODS FOR THE RAPID DETECTION OF ANALYTES - This invention features systems and methods for the detection of analytes, and their use in the treatment and diagnosis of disease. | 04-17-2014 |
20140113357 | REMOTE CHEMICAL ASSAY SYSTEM - A portable device for remote chemical assay classification, comprising a computer processor, and an apparatus implemented on the computer processor, the apparatus comprising: an out-of-sample data receiver, configured to receive data defining an out-of-sample extension extracted on a remote computer from classifying test assays of a chemical reaction on the remote computer into at least two groups, and an assay classifier, in communication with the out-of-sample data receiver, configured to classify a new assay of the chemical reaction into one of the groups, using the data defining the out-of-sample extension. | 04-24-2014 |
20140120603 | HIGH SENSITIVTY MEDICAL DEVICE AND MANUFACTURING THEREOF - This invention relates to a system and methods including their manufacturing technologies for enhanced sensing capability of one or more bioagents covering from HIV, Pathogens, virus, to cells detection. More particularly, this invention is related to HIV and pathogen diagnosis system and methods which may increase its sensitivity and may reduce the diagnosis time. Furthermore, the diagnosis system and method may be applicable to all early stage patients with various age groups, where early and accuracy in diagnosis, are required. | 05-01-2014 |
20140120604 | Biological Detection System and Method of Use - Provided herein is a biological detection system and method of use wherein the biological detection system comprises at least one mixer or liquid bridge for combining at least two liquid droplets and an error correction system for detecting whether or not proper mixing or combining of the two component droplets have occurred. | 05-01-2014 |
20140127792 | SEQUENCE SCHEDULING AND SAMPLE DISTRIBUTION TECHNIQUES - A technique is disclosed for sample management for use in conjunction with sequencing devices that sequence biological samples, e.g., DNA and RNA. A sequencing device or a network of sequencing devices may be scheduled according to the characteristics of the samples in queue and the capabilities and availability of sequencing devices. Biological samples may be automatically queued and loaded via a sample distribution system. A sample distribution system may be used to reduce operator intervention. | 05-08-2014 |
20140127793 | ALLELIC DISCRIMINATION ANALYSIS USING AN EFFICIENCY RELATED VALUE (EFR) - In various embodiments this invention provides novel methods for discriminating two or more different target nucleic acids. In certain embodiments the methods comprise providing data amplification reactions comprising reagents to amplify two or more different target nucleic acids where the data comprise signals comprising an amplitude measurement representing the degree of amplification of each target nucleic acid in the amplification reaction and the time point in the amplification reaction at which the amplitude is measured; determining an efficiency related transform of the data, determining an efficiency related value for each target nucleic acid that is the maximum magnitude of the efficiency related transform; and outputting the efficiency related values in the amplification reaction for each target nucleic acid, where the relative amplitudes of the efficiency related values for each target nucleic acid is an indicator of the presence of each of said nucleic acids in said sample. | 05-08-2014 |
20140141501 | Fluidic Indicator Device - Disclosed is a fluidic assay device for assaying at least one property of a liquid sample, the device comprising:
| 05-22-2014 |
20140141502 | Methods For Concurrent Identification And Quantification Of An Unknown Bioagent - The present invention provides methods for the quantification of an unknown bioagent in a sample by amplification of nucleic acid of the bioagent, and concurrent amplification of a known quantity of a calibration polynucleotide from which are obtained a bioagent identifying amplicon and a calibration amplicon. Upon molecular mass analysis, mass and abundance data are obtained. The identity of the bioagent is then determined from the molecular mass of the bioagent identifying amplicon and the quantity of the identified bioagent in the sample is determined from the abundance data of the bioagent identifying amplicon and the abundance data of the calibration amplicon. | 05-22-2014 |
20140154786 | Chemical Temperature Control - Exothermic and/or endothermic chemical reactions in combination with phase change materials can produce output temperature(s) within strict tolerances without requiring expensive and complicated external equipment to generate and maintain an output temperature. Similarly, an exothermic phase change material, which generates heat as a consequence of crystallizing a supercooled liquid, can generate heat at a constant temperature, without requiring expensive and complicated external equipment, as a consequence of the liquid form of the exothermic phase change material being in equilibrium with the solid form of the exothermic phase change material. Numerous biological and chemical processes and/or diagnostic devices require a constant temperature or temperatures for set periods of time. An example completely non-instrumented diagnostic platform based on nucleic acid amplification is described, which is particularly suited for use in developing countries that may not have access to expensive and complicated external equipment. | 06-05-2014 |
20140154787 | METHOD AND APPARATUS FOR BIOLOGICAL SAMPLE ANALYSIS - A method of adjusting amplification curves in a PCR experiment includes receiving a plurality of amplification curves for a sample and computing a first parameter for each of the plurality of amplification curves. The method also includes computing a second parameter for each of the plurality of amplification curves and computing a third parameter using at least a portion of the first or second parameters. The method further includes computing an offset for each of the plurality of amplification curves. The offset is a function of the first parameter and the third parameter. Moreover, the method includes adjusting at least one of the plurality of amplification curves by subtracting the offset. | 06-05-2014 |
20140154788 | ELECTRONIC COMPONENTS ON PAPER-BASED SUBSTRATES - The present disclosure relates to paper-based substrates and apparatus comprising such substrates. The apparatus may include a patterned conductive structure coupled to the paper-based substrate, wherein the patterned conductive structure responds to electromagnetic radiation. | 06-05-2014 |
20140154789 | TESTING APPARATUS - A testing apparatus for performing an assay, the testing apparatus comprising: a receptacle ( | 06-05-2014 |
20140154790 | BIOMOLECULE INFORMATION ANALYSIS DEVICE - Provided is a device that, on the basis of a measurement result of a current that has a low value and a wide distribution, identifies the composition of biological molecules passing through a nanoparticle path. This biomolecule information analysis device obtains a current value by applying an electrical field to biomolecules passing through a gap between a first electrode and a second electrode, and identifies the structure of the biomolecules by integrating the current value and making a comparison with a reference value (see FIG. | 06-05-2014 |
20140154791 | PROCESSING MICROTITRE PLATES FOR COVALENT IMMOBILIZATION CHEMISTRIES - Disclosed herein is a method of: treating an organic polymer with an electron beam-generated plasma; exposing the treated polymer to air or an oxygen- and hydrogen-containing gas, generating hydroxyl groups on the surface of the polymer; reacting the surface with an organosilane compound having a chloro, fluoro, or alkoxy group and a functional or reactive group that is less reactive with the surface than the chloro, fluoro, or alkoxy group; and covalently immobilizing a biomolecule to the functional or reactive group or a reaction product thereof. | 06-05-2014 |
20140154792 | READER DEVICE FOR LUMINESCENT IMMUNOASSAYS - The present disclosure, among other things, describes a reader system for analyzing one or more analytes in a fluid sample, comprising a casing ( | 06-05-2014 |
20140170736 | METHODS AND COMPOSITIONS FOR USING OILS FOR ANALYSIS AND DETECTION OF MOLECULES - Oil compositions and methods for use in droplet formation or in spacing of droplets are disclosed. The oil compositions may include a combination, such as a silicone oil and a fluorine-containing compound. The droplets can contain analytes (e.g., nucleic acids) and may be used for molecular reactions (e.g., digital PCR) and detection. | 06-19-2014 |
20140170737 | STACKABLE MICRO-FLUIDIC CELLS - An assay assembly that includes an assay bar having a plurality flow cells is disclosed. Each of the flow cells includes an inlet, an outlet, and an inside surface defining an inner volume. The outlet includes a valve that is configured to retain liquid within the inner volume of the flow cell. Each assay bar is configured to be reversibly stacked upon another assay bar, such that the flow cells of the stacked assay bars are in fluid communication with each other. This way, the outlet of a first flow cell of a first assay bar is in fluid communication with the inlet of a second flow cell of a second assay bar. The assay assembly may include a multitude of assay bars to form a composite assay assembly, with the flow cells of the stacked assay bars being in fluid communication with each other. | 06-19-2014 |
20140178975 | DETECTION OF EARLY STAGES AND LATE STAGES HPV INFECTION - Embodiments of the invention provide methods, monoclonal antibodies, polyclonal antibodies, assays, and kits for detecting HPV infection and HPV related cancer diagnosis, including infection by various HPV genotypes, early and/or late stage HPV-associated or HPV-specific cancers. Various specific or pan monoclonal antibodies recognizing specific epitope for specific HPV protein or HPV type, or common epitope for various HPV proteins or HPV types are obtained. The invention also provides one or more solid surface to coat the testing cell lysate. Also, the anti-HPV antibody can be coated on the solid surface of the invention to capture HPV proteins and detect HPV infection. | 06-26-2014 |
20140178976 | IMMUNOASSAY TEST STRIP FOR USE IN A DIAGNOSTIC SYSTEM - An immunoassay test strip includes a sample pad for receiving a liquid patient sample; a conjugate pad fluidly coupled to the sample pad, wherein the conjugate pad contains a substantially uniform application of conjugate reagent; a contact pad fluidly coupled to the conjugate pad; a porous or bibulous member, e.g., made from nitrocellulose, fluidly coupled to the contact pad which is capable or transporting a liquid sample along the test strip, wherein the porous or bibulous member serves as the solid support upon which immunoreactions occur, and an absorbent pad fluidly coupled to the porous or bibulous member, which serves to draw sample fluid introduced onto the sample pad through the respective conjugate pad, contact pad and porous or bibulous member. | 06-26-2014 |
20140178977 | SYSTEMS AND METHODS FOR DETECTING MULTIPLE OPTICAL SIGNALS - To minimize cross talk in systems and methods for detecting two or more different optical signals emitted from each of a plurality of reaction receptacles, an excitation signal associated with each of the optical signals has a known excitation frequency, and any detected signal having a frequency that is inconsistent with the excitation frequency is discarded. The receptacles are moved relative to optical sensors configured to detect each unique optical signal from an associated receptacle, and to further minimize cross talk, the optical sensors are arranged so that only one reaction receptacle at a time is in a signal detecting position with respect to one of its associated optical sensors, and the optical sensors are grouped by the optical signal they are configured to detect so that a first optical signal is detected from each of the reaction receptacles before a second optical signal is detected from the reaction receptacles. | 06-26-2014 |
20140186933 | Method and device for detecting prior drug use, taking inflammation into account to increase test's specificity and reduce false positives - This method and device detects past use of drugs of abuse when the drug substance and its metabolites are no longer present in the body, based on the detection of the antibodies formed in response to said drug, and persist even after this drug and its metabolites are cleared from the body. False positive rate is reduced by screening out subjects with heightened inflammatory state. One embodiment of the invention sets forth the method and device comprising: testing a set of samples for levels of an inflammation biomarker; determining a subset of samples with inflammation biomarker level below a first predetermined threshold; testing samples within the subset of samples for a presence of drug-specific antibodies, for a pre-specified set of drugs; determining which samples within the subset have drug-specific antibody level exceeding pre-specified threshold; and providing an output indicating the samples within the subset having the drug-specific antibodies. | 07-03-2014 |
20140186934 | Rapid Diagnostic Device, Assay and Multifunctional Buffer - An improved rapid diagnostic device, assay and multifunctional buffer reagent are provided for the detection of a target analyte in a fluid test sample. The 2-step assay utilizes a dual component flow-through device comprising a test unit and a dried indicator reagent delivery unit capable of receiving the fluid sample and multifunctional buffer, respectively. The test unit comprises a reaction zone containing immobilized capture reagent that can specifically bind to the target analyte, an absorbent zone supporting the reaction zone, and optionally, a blood separation zone in lateral fluid communication with the reaction zone. The dried indicator reagent delivery unit comprises a label zone permeated with a dried indicator reagent which is capable of being placed in transient fluid communication with the reaction zone of the test unit during the assay procedure. The rapid diagnostic assay system reduces the number of assay reagents, method steps and time required for performance compared to other conventional assays. | 07-03-2014 |
20140186935 | THIN-FILM LAYERED CENTRIFUGE DEVICE AND ANALYSIS METHOD USING THE SAME - Disclosed herein is a thin-film layered centrifuge device and an analysis method using the same. One example of an embodiment of the present invention is a thin film layered centrifuge device where a device, such as a lab on a chip, a protein chip and a DNA chip, for diagnosing and detecting a small amount of material in a fluid is integrated into a rotatable thin-film layered body, and to an analysis method using the thin-film layered centrifuge device. | 07-03-2014 |
20140186936 | DNA CHIP WITH MICRO-CHANNEL FOR DNA ANALYSIS - A DNA chip with micro-channel for DNA analysis of DNA included in an analyte according to a PCR method is a DNA chip with micro-channel for DNA analysis in which is silicon (first layer) and plastic (second layer) are laminated, wherein the second layer is formed on a partial area of the first layer, and the second layer includes: a reagent; a liquid transporting system; and a sensor, and the first layer includes a PCR reactor provided on an area on which the second layer is not formed. | 07-03-2014 |
20140193891 | METHODS FOR DETECTION OF BIOLOGICAL SUBSTANCES - Methods and compositions are provided for detection of biological substances in nasal specimen. | 07-10-2014 |
20140193892 | IMAGE ANALYSIS AND MEASUREMENT OF BIOLOGICAL SAMPLES - Methods, devices, apparatus, and systems are provided for image analysis. Methods of image analysis may include observation, measurement, and analysis of images of biological and other samples; devices, apparatus, and systems provided herein are useful for observation, measurement, and analysis of images of such samples. The methods, devices, apparatus, and systems disclosed herein provide advantages over other methods, devices, apparatus, and systems. | 07-10-2014 |
20140193893 | GENETIC TEST SYSTEM - There is provided a genetic test system provided with a dispensing means for dispensing a sample and a reagent to a reaction vessel and a vessel conveyance means for conveying the reaction vessel. The system further includes a plurality of nucleic acid amplification detection units, each including a temperature control means for accommodating a plurality of reaction vessels and performing temperature control for each accommodated position of the reaction vessel, a temperature monitoring means for monitoring a value of temperature to be controlled of the reaction vessel, and a light emission measurement means for measuring light emission of reaction liquid in the reaction vessel. At least one of the nucleic acid amplification detection units individually has a vessel conveyance means different from the above-mentioned vessel conveyance means. | 07-10-2014 |
20140206073 | MICROFLUIDIC SYSTEM FOR NUCLEIC ACID ANALYSIS - A microfluidic system for analyzing nucleic acid, the microfluidic system including a reagent supply device including a sample chamber into which a sample can be injected, one or more reagent chambers for containing one or more reagents for extracting nucleic acid from the sample, and a waste chamber in which the used reagent can be discarded; a binding-lysis chamber in which cells are captured from the sample and lysed to form a cell lysate containing nucleic acid; plurality of particles for cell binding disposed in the binding-lysis chamber; a plurality of rehydration chambers into which the cell lysate formed in the binding-lysis chamber can be distributed and mixed with a nucleic acid amplification reagent to form an amplification reaction mixture; a plurality of amplification chambers in which a nucleic acid amplification reaction is performed on the amplification reaction mixture introduced from the plurality of rehydration chambers; and a flow channel system including an outlet and a plurality of inlets connected to the reagent supply device and forming an integrated fluid flow between the binding-lysis chamber, the rehydration chambers, and the amplification chambers. | 07-24-2014 |
20140206074 | CARTRIDGE FOR CONDUCTING A CHEMICAL REACTION - A cartridge for conducting a chemical reaction includes a body having at least one flow path formed therein. The cartridge also includes a reaction vessel extending from the body for holding a reaction mixture for chemical reaction and optical detection. The vessel comprises a rigid frame defining the side walls of a reaction chamber. The frame includes at least one channel connecting the flow path to the chamber. The vessel also includes flexible films or sheets attached to opposite sides of the rigid frame to form opposing major walls of the chamber. In addition, at least two of the side walls are optically transmissive and angularly offset from each to permit real-time optical detection of analyte in the reaction chamber. | 07-24-2014 |
20140206075 | VOLTAGE GATED METAL-ENHANCED FLUORESCENCE, CHEMILUMINESCENCE OR BIOLUMINESCENCE METHODS AND SYSTEMS - In the present invention, it is demonstrated for the first time, the influence of electrical current on the ability of surface plasmons to amplify fluorescence signatures. An applied direct current across silver island films (SiFs) of low electrical resistance perturbs the fluorescence enhancement of close-proximity fluorophores. For a given applied current, surface plasmons in “just-continuous” low resistance films are sparsely available for fluorophore dipole coupling and hence the enhanced fluorescence is gated as a function of the applied current. | 07-24-2014 |
20140206076 | MICRO-FLUIDIC DEVICE - Embodiments described herein provide micro-fluidic systems and devices for use in performing various diagnostic and analytical tests. According to one embodiment, the micro-fluidic device includes a sample chamber for receiving a sample, and a reaction chamber for performing a chemical reaction. A bubble jet pump is structured on the device to control delivery of a fluid from the sample chamber to the reaction chamber. The pump is fluidically coupled to one or more chambers of the device using a fluidic channel such as a capillary. A valve may be coupled to one or more chambers to control flow into and out of those chambers. Also, a sensor may be positioned in one or more of the chambers, such as the reactant chamber, for sensing a property of the fluid within the chamber as well as the presence of a chemical within the chamber. | 07-24-2014 |
20140212958 | LYSIS AND REVERSE TRANSCRIPTION FOR MRNA QUANTIFICATION - The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA including the steps of (i) cultivation of a population of adherent cells in a cell culture vessel (ii) lysis of the population of adherent cells which is supposed to contain the target RNA in the sample vessel with a lysis buffer comprising between 0.05 M and 1 M of a chaotropic agent (iii) adding reagents to the sample vessel which are necessary to perform a reverse transcription reaction such that the chaotropic agent is present in a concentration of about 10 to 60 mM in the sample vessel, and reverse transcribing the target RNA and (iv) amplifying the first strand cDNA by means of subjecting the sample to multiple cycles of a thermocycling protocol. | 07-31-2014 |
20140220667 | Device for pathogen detection - There is provided a portable device for detecting pathogens in a sample, the detection being based on an assay involving one or more reagents and heat. The device comprises a flexible substrate having a plurality of spaced apart reservoirs along its length, each reservoir adapted for receiving reagents and the sample, the substrate being secured to a pair of spaced apart reels and movable there between by rolling. Also, the device comprises heating means which can be secured to one of the two reels. | 08-07-2014 |
20140220668 | MICROFLUIDIC DEVICE - To provide a microfluidic device which does not apply a meandering reaction channel and thus can be reduced in size, the microfluidic device comprising a reaction channel is characterized in that a plurality of thermal cycle regions which respectively comprise at least two thermal regions with different temperatures are repeatedly provided and the reaction channel passes through the plurality of thermal cycle regions. | 08-07-2014 |
20140220669 | INSTRUMENT AND METHOD FOR THE AUTOMATED THERMAL TREATMENT OF LIQUID SAMPLES - An instrument and a method for the automated thermal treatment of liquid samples are disclosed. An inter-distance between a temperature-controlled receptacle for loading with a plurality of vessels for containing the samples and end portions of optical fibers can be varied, wherein the receptacle is configured to form a thermal communication with the loaded vessels and wherein the optical fibers have first and second end portions. The first end portion and the second end portion of each optical fiber is fixed with respect to each other for transmitting light, wherein the variation of the inter-distance allows the vessels to be loaded to or unloaded from the receptacle and to enable detection of light from the samples contained in the one or more receptacle-loaded vessels. | 08-07-2014 |
20140234949 | SYSTEMS AND METHODS FOR FLUID AND COMPONENT HANDLING - Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample. | 08-21-2014 |
20140242682 | Salivary Diagnostic Systems - Salivary diagnostic systems are provided for improved disease diagnosis and overall health care. Salivary diagnostic systems are provided for the plurality of steps required in salivary diagnostic processes. The salivary diagnostic systems are interchangeably coupled by various methods and steps and include a collection system, a filter system, an evaluation system, and a reporting system. One or more salivary diagnostic systems are comprised in at least one salivary diagnostic device. Salivary diagnostic devices include a toothbrush, an implement, and a stand-alone system. The systems provide for the collection, filtration, and evaluation of saliva samples and the reporting of diagnostic data derived from the evaluation of saliva samples. | 08-28-2014 |
20140242683 | APPARATUS FOR SELECTIVE EXCITATION OF MICROPARTICLES - Nucleic acid microparticles are sequenced by performing a sequencing reaction on the microparticles using one or more reagents, selectively exciting the microparticles in an excitation pattern, optically imaging the microparticles at a resolution insufficient to resolve individual microparticles, and processing the optical images of the microparticles using information on the excitation pattern to determine the presence or absence of the optical signature, which indicates the sequence information of the nucleic acid. An apparatus for optical excitation of the microparticles comprises an interference pattern generation module that splits a first laser beam into second and third laser beams and generates the excitation pattern for selectively exciting the microparticles by interference between the second and third laser beams. | 08-28-2014 |
20140248691 | Caps for Sample Wells and Microcards for Biological Materials - A cover for a biological sample well tray, comprising a cap for sealing a sample well. The cap comprises a well lens for focusing light into the sample well and collecting light from the sample. In another aspect, the cap comprises an elongate portion configured to permit incoming light to pass into the sample well and out of the sample well. Various other aspects comprise a microcard for biological material, and an apparatus for a plurality of sample well strips. A method for testing a biological sample is also provided. | 09-04-2014 |
20140248692 | SYSTEMS AND METHODS FOR NUCLEIC ACID-BASED IDENTIFICATION - Systems and methods for calculating a predictive index of identity of a nucleic acid sample using polymorphic genetic marker data are provided. In one embodiment, a predictive index of identity of the nucleic acid sample is calculated using a value from a second set of data from a polymorphic genetic marker that is not linked to a polymorphic genetic marker used to produce a first set of data. In another embodiment, the predictive index of identity is calculated using a value from a second set of data from a polymorphic genetic marker that is linked to a polymorphic genetic marker used to produce the first set of data. Systems and methods for generating an identifier for a biological sample and for verifying a relationship between a biological sample and an identifier are also provided. The identifier is an encoding of a set of values for polymorphic genetic markers. | 09-04-2014 |
20140248693 | Optical Systems and Methods for Biological Analysis - An instrument for processing and/or measuring a biological process contains an excitation source, a sample holder, an optical sensor, an excitation optical system, and an emission optical system. The sample holder is configured to receive a plurality of biological samples. The optical sensor is configured to receive an emission from the biological samples. The excitation optical system is disposed along an excitation optical path and is configured to direct the electromagnetic radiation from the excitation source to the biological samples. The emission optical system is disposed along an emission optical path and is configured to direct electromagnetic emissions from the biological samples to the optical sensor. The instrument further contains a plurality of filter assemblies configured to be interchangeably located along at least one of the optical paths. The plurality of filter components includes a first filter assembly characterized by a first optical power and a first filter having a first filter function, the first filter function characterized by at least one of a first low-pass wavelength or a first high-pass wavelength. The second filter assembly is characterized by a second optical power and a second filter having a second filter function, the second filter function comprising at least one of a second low-pass wavelength that is different than the first low-pass wavelength or a second high-pass wavelength that is different than the first high-pass wavelength. | 09-04-2014 |
20140256029 | APPARATUS FOR POLYNUCLEOTIDE DETECTION AND QUANTITATION - An apparatus for expression profiling analysis, subjecting biological materials to polynucleotide extraction, amplification and analysis. The apparatus include an amplification device which permits the amplification of polynucleotides and an analysis device which quantifies the amount of the amplified polynucleotide products. The amplification device of the apparatus may further permit polynucleotide extraction to prepare the template for amplification, or sequence identification of a quantified polynucleotide product. A fraction collector may be included in the apparatus to collect a qualified polynucleotide product before its sequence is identified. The analysis device may further permit data generation, or alternatively, data can be generated by a separate data generation device provided with the apparatus. The devices within the apparatus are connected by connecting means which permit the transfer of a fluid or a signal for amplification and analysis. | 09-11-2014 |
20140273184 | ELECTROKINETIC DEVICES AND METHODS FOR CAPTURING ASSAYABLE AGENTS - Electrokinetic devices and methods are described with the purpose of collecting assayable agents from a dielectric fluid medium. Electrokinetic flow may be induced by the use of plasma generation at high voltage electrodes and consequent transport of charged particles in an electric voltage gradient. Pulsed DC fields applied to electrodes result in enhanced flow by synchronizing the pulses between successive electrodes. The agents are directed by creation of an electrokinetic potential well, which will effect their capture on to an assay device. | 09-18-2014 |
20140273185 | Tactical and Portable PCR/HRM Genome Identification System - The present invention relates to a Polymerase Chain Reaction and High Resolution Melt genetic identification system, and, more specifically, to a tactical and portable Polymerase Chain Reaction and High Resolution Melt genetic analysis and identification system that is configured to determine and communicate analysis and identification results and a tiered confidence/alert level related to the analysis and identification results. | 09-18-2014 |
20140273186 | METHOD AND APPARATUS FOR SEQUENCING MOLECULES - The invention comprises a polymer sequencing chip. In one embodiment, an open nano channel is used in the surface of a semiconductor material or glass as a conduit for the polymer and the resulting monomers released from the polymer. As the resulting monomers pass through the nano channel, a detection device reads the sequence of monomers. Preferably, multiple micro LEDs are used to emit a signal when different monomer units pass through a region of the nano channel, such that multiply parallel sequencing operations can be conducted at the same time on a chip. | 09-18-2014 |
20140273187 | POINT OF CARE SENSOR SYSTEMS - Provided are point of care sensor systems that include portable readers and disposable cartridges for receiving and analyzing samples. A cartridge may be equipped with one or more sensor channels, each containing one or more sensors. After providing a sample to a cartridge, the cartridge can be inserted into a reader, which can interact with the cartridge to perform on-cartridge sensing and receive signals indicating the presence and/or quantity of one or more targets in the sample. Examples of cartridges can include cardiac panels, sepsis panels and the like. In some embodiments, the same sensor hardware may be configured for multiple measurements of different targets conducted at different time frames. Also provided herein are novel on-cartridge solid and liquid reagent storage and delivery mechanisms. | 09-18-2014 |
20140273188 | IMAGE ANALYSIS AND MEASUREMENT OF BIOLOGICAL SAMPLES - Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples. | 09-18-2014 |
20140273189 | TEST STRIP DETECTION SYSTEM - A test strip detection system, comprising a test strip card ( | 09-18-2014 |
20140287486 | DEVICE FOR ANALYSIS OF A TARGET ANALYTE - This disclosure is directed to a slide for analyzing target analytes of a suspension. The slide can be used to collect and hold the target analyte for imaging or further processing. The slide comprises a well section, including wells into which the target analyte can be stored. The wells may be removable and sized to fit into a second apparatus, such as a PCR thermocycler, for additional processing. Alternatively, the wells may hold processing vessels, such as PCR tubes, the processing vessels being used for downstream processing and/or analysis. | 09-25-2014 |
20140295533 | Enhanced Microfluidic Valves for Media Diagnostics - The invention provides a micro-fluidic device that includes a pre-loaded sample that begins to flow only when a sample is provided in the device. In certain embodiments, the invention provides a micro-fluidic device including at least two paper flow path layers providing fluid flow in substantially parallel planar directions, and at least one valve positioned between the at least two paper flow paths, wherein the at least one valve for providing flow in a direction that is orthogonal to the substantially planar directions. | 10-02-2014 |
20140295534 | SYSTEMS AND DEVICES FOR ISOTHERMAL BIOCHEMICAL REACTIONS AND/OR ANALYSIS - An isothermal reaction and analysis system may include a receiver to receive sample holders, a thermal control subsystem to control a temperature of the receiver, an excitation subsystem, a detection subsystem and an analysis subsystem. Excitation sources and/or detectors are positioned to enhance data collection. Sample holders may include filters, selectively blocking and passing wavelengths or bands of electromagnetic radiation. | 10-02-2014 |
20140315283 | SAMPLE CARTRIDGE AND SAMPLE STAGE - A sample cartridge ( | 10-23-2014 |
20140315284 | ENHANCED DETECTION SENSITIVITY WITH PIEZOELECTRIC MICROCANTILEVER SENSORS - A method for enhancing the detection sensitivity of a piezoelectric microcantilever sensor. The method may involve providing a piezoelectric microcantilever and inducing a change in the Young's modulus during detection of a species of interest. The change in the Young's modulus may be induced or enhanced by the application of a DC bias electric field to the piezoelectric layer that enhances non-180° polarization domain switching of the piezoelectric layer. The change in the Young's modulus may also result from binding of the species of interest to the piezelectric microcantilever sensor or a combination of binding and application of a DC bias electric field. Significantly enhanced detection sensitity results from the changed Young's modulus of the piezoelectric layer. | 10-23-2014 |
20140322800 | OXIDIZED CARDIOLIPIN AND USES TO DETECT CARDIOLIPIN ANTIBODIES - Compositions, methods and devices for the detection of anti-lipoidal antibodies and the diagnosis of disease, for example, syphilis, are described. In particular, oxidized cardiolipins, which may be conjugated with a variety of attachment molecules, such as BSA, KLH, biotin, synthetic protein MAPS, IgY, streptavidin, or avidin, are described. Such oxidized cardiolipin, alone or complexed with one or more attachment molecules, are useful to detect anti-lipoidal antibodies (such as IgG and IgM antibodies) in subjects, for example, when used in ELISA plates. ELISA plates are described that permit the detection of anti-lipoidal antibodies and that permit the co-detection of nontreponemal and treponemal antibodies in biological samples. | 10-30-2014 |
20140329300 | DEVICE FOR ANALYZING A SAMPLE AND METHOD OF USING THE SAME - This disclosure is directed to a device and method of using the device to analyze a sample. The sample may be a suspension, a portion of the suspension, a particular component of the suspension, or the like. For example, the suspension may be blood, the portion may be buffy coat, and the component may be circulating tumor cells. The device may be used to hold the sample for imaging or further processing. The device comprises a cavity that may be sealed on one side by a porous membrane and on an opposite side by a non-porous cover. The porous membrane allows for reagents to be introduced to the sample without diluting the sample. The non-porous cover allows for imaging of the sample. The device further comprises a nozzle for introducing the sample into a space within the cavity. | 11-06-2014 |
20140329301 | MULTIPLEXED DIAGNOSTIC DETECTION APPARATUS AND METHODS - Disclosed herein are devices configured for the amplification and detection of multiple targets from a sample, and methods of using the same. The devices disclosed herein comprise microfluidic cartridges have a first stage (amplification) and a second (detection) stage. The two-stage design of the cartridges enables testing for multiple targets within a sample, i.e., from a single nucleic acid amplification reaction. Methods for the amplification and detection of a plurality of target nucleic acids from a sample are also disclosed herein. | 11-06-2014 |
20140329302 | WIRELESS BASED MARINE PATHOGENS DETECTION SYSTEM - Embodiments of the present invention provide antibody functionalized high electron mobility transistor (HEMT) devices for marine or freshwater pathogen sensing. In one embodiment, the marine pathogen can be | 11-06-2014 |
20140329303 | In-vitro contact lens testing - Contact lens testing apparatuses and method for testing contact lenses for analytes are presented. In an aspect, a device is provided that includes a housing configured to hold one or more contact lenses, and a testing compartment provided within the housing and comprising a reagent, the reagent configured to facilitate a chemical reaction in response to the existence of a predetermined biomarker disposed on or within a contact lens placed in the testing compartment, wherein the chemical reaction produces a known result related to state information of an individual from which the biomarker was generated. | 11-06-2014 |
20140335603 | METHOD FOR SIGNAL AMPLIFICATION IN BIOSENSOR-BASED SYSTEM FOR RAPIDLY DETECTING INFECTIOUS AGENTS - A system for detecting infectious agents in biological samples in real time that includes a sample to be tested for at least one specific infectious agent; and a biosensor, wherein the biosensor is operative to detect a specific infectious agent in the sample to be tested; and wherein the biosensor emits an amplified and detectable signal when it reacts with the specific infectious agent. Signal detection is enhanced through the release of LPS from the cells of the infectious agent. | 11-13-2014 |
20140342441 | APPARATUS FOR DISEASE DETECTION - The present invention provides, among others, apparatus for detecting a disease, comprising a system delivery biological subject and a probing and detecting device, wherein the probing and detecting device includes a first micro-device and a first substrate supporting the first micro-device, the first micro-device contacts a biologic material to be detected and is capable of measuring at the microscopic level an electric, magnetic, electromagnetic, thermal, optical, acoustical, biological, chemical, physical, or mechanical property of the biologic material. | 11-20-2014 |
20140342442 | TOUCHSCREEN DEVICE AND METHODS FOR USE IN DETECTION OF MICRORNA - A method and sensor for detecting the presence of specific target nucleic acids uses a biosensor comprising an array of single stranded probe oligonucleotides, wherein one end of each probe oligonucleotide is linked to a label and the other end of each probe oligonucleotide is attached to an electrode of a solid support. The method includes determining the capacitance at one or more of the electrodes, where a change in the capacitance of the one or more electrodes indicates the presence of target nucleic acid complementary to the single stranded oligonucleotide probe. | 11-20-2014 |
20140349380 | BIOPOLYMER OPTOFLUIDIC DEVICE AND METHOD OF MANUFACTURING THE SAME - A method of manufacturing a biopolymer optofluidic device including providing a biopolymer, processing the biopolymer to yield a biopolymer matrix solution, providing a substrate, casting the biopolymer matrix solution on the substrate, embedding a channel mold in the biopolymer matrix solution, drying the biopolymer matrix solution to solidify biopolymer optofluidic device, and extracting the embedded channel mold to provide a fluidic channel in the solidified biopolymer optofluidic device. In accordance with another aspect, an optofluidic device is provided that is made of a biopolymer and that has a channel therein for conveying fluid. | 11-27-2014 |
20140349381 | PORTABLE HIGH GAIN FLUORESCENCE DETECTION SYSTEM - An instrument for fluorometric assays in liquid samples is disclosed. The instrument may include multiple optical channels for monitoring a first fluorophore associated with a target analyte and a second fluorophore associated with a control. The disclosed instrument finds utility in any number of applications, including microfluidic molecular biological assays based on PCR amplification of target nucleic acids and fluorometric assays in general. | 11-27-2014 |
20140356935 | INFORMATION NOTIFICATION SAMPLE PROCESSING SYSTEM AND METHODS OF BIOLOGICAL SLIDE PROCESSING - A sample processing system that may be automated and methods are disclosed where samples are arranged on a carrier element and a process operation control system automatically processes the samples perhaps robotically with an operationally-influential exteriorly-consequential information monitor or a data capture element. Significant process details as well as operationally-influential exteriorly-consequential information may be monitored and an automatic notice element may cause notification of a person at some display that may be remote. Various people may be notified, such as an administrator, a supplier, or a manufacturer of an opportunity for some action such as reagent reordering or the like. A simulated motion display may be included to “watch” simulated operation in real time or long after completion of the actual processing. | 12-04-2014 |
20140356936 | METHODS FOR NUCLEIC ACID MANIPULATION - A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems. | 12-04-2014 |
20140363882 | RAPID DETECTION OF ANALYTES IN LIQUID SAMPLES - A device for detecting at least one analyte in a liquid sample generally comprises (i) a support having a chamber for receiving a biological fluid (e.g., milk) therein, wherein said chamber is an elongate chamber having a length axis; (ii) a (stationary or movable) carrier (in some embodiments in the form of an end cap, or connected to an end cap; in other embodiments in the form of an agitator in said elongate chamber). | 12-11-2014 |
20140370580 | Unitary Biochip Providing Sample-in to Results-out Processing and Methods of Manufacture - A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided. | 12-18-2014 |
20140370581 | MICROFLUIDIC DEVICES FOR FLUID MANIPULATION AND ANALYSIS - The present invention relates to microfluidic devices and methods for manipulating and analyzing fluid samples. The disclosed microfluidic devices utilize a plurality of microfluidic channels, inlets, valves, filter, pumps, liquid barriers and other elements arranged in various configurations to manipulate the flow of a fluid sample in order to prepare such sample for analysis. | 12-18-2014 |
20140370582 | SYSTEM AND METHOD FOR DETERMINING INDIVIDUALIZED MEDICAL INTERVENTION FOR A DISEASE STATE - A system and method for determining individualized medical intervention for a particular disease state, and especially for cancers, that includes the molecular profiling of a biological sample from the patient, determining whether any molecular findings including one or more genes, one or more gene expressed proteins, one or more molecular mechanisms, and/or combinations of such exhibit a change in expression compared to a reference, and identifying a non-specific disease therapy or agent capable of interacting with the genes, gene expressed proteins, molecular mechanisms, or combinations of such molecular findings that exhibited a change in expression. | 12-18-2014 |
20140377852 | Methods and Systems for Epi-Fluorescent Monitoring and Scanning for Microfluidic Assays - An operating and reading instrument for performing an assay employing a portable microfluidic assay cartridge, the instrument comprising a translatable table under automated control, the translatable table carrying a receiving region for the portable cartridge and carrying a port system connectible to the cartridge that includes at least one remotely automated valve carried by the translatable table, the valve arranged to apply pressurized flowable substance at selected times to the cartridge while the cartridge is on the translatable table. | 12-25-2014 |
20150010991 | BIO-SENSING DEVICE - A bio-sensing device includes a first platform, a second platform, at least one first magnetic element, and at least one second magnetic element. The first platform is configured to support a microplate. The microplate has a plurality of wells, each of which stores a reagent and a plurality of microbeads. The second platform is movably placed below the first platform. The first and the second magnetic elements are respectively located on the second platform. The first magnetic element moves along with the second platform to magnetically attract the microbeads in the wells, the second magnetic element moves along with the second platform to demagnetize the microbeads in the wells, and magnetism of the first magnetic element is opposite to magnetism of the second magnetic element. | 01-08-2015 |
20150010992 | OPTICAL READER SYSTEMS AND LATERAL FLOW ASSAYS - Systems and methods for determining the presence and/or amount of analytes in a fluid sample are described. | 01-08-2015 |
20150024474 | THERMAL CYCLER DEVICE - The present invention relates to a thermal cycler device for carrying reaction slides for assays with thermal cycling reactions. The thermal cycler device includes a conveyer with a plurality of slide holders for conveying slide plates through more than one temperature zones for thermal cycling reactions. | 01-22-2015 |
20150024475 | AFFINITY CAPTURE OF CIRCULATING BIOMARKERS - Methods, devices and systems for capturing biomarkers are provided. In particular, methods, compositions, and systems that utilize affinity capture devices comprising a processing chamber, affinity capture agent and porous membrane are provided. | 01-22-2015 |
20150031117 | METHODS FOR RAPID FORENSIC ANALYSIS OF MITOCHONDRIAL DNA AND CHARACTERIZATION OF MITOCHONDRIAL DNA HETEROPLASMY - The present invention provides methods for rapid forensic analysis of mitochondrial DNA and methods for characterizing heteroplasmy of mitochondrial DNA, which can be used to assess the progression of mitochondrial diseases. | 01-29-2015 |
20150037876 | MULTI-CHANNEL FLUORESCENCE DETECTING MODULE AND NUCLEIC ACID ANALYSIS SYSTEM HAVING THE SAME - Provided are a multi-channel fluorescence detecting module and a nucleic acid analysis system including the multi-channel fluorescence detecting module. The nucleic acid analysis system includes: a plurality of loaders configured to accommodate a plurality of cartridges respectively, the cartridges comprising microfluidic devices; a transfer module including a linear actuator, the linear actuator including a movable unit configured to move linearly; and a fluorescence detecting module fixed to the movable unit, the fluorescence detecting module being configured to emit excitation light to the cartridges and detect fluorescence emitted from samples on the cartridges. The loaders are arranged in a row in a linearly moving direction of the movable unit. | 02-05-2015 |
20150044758 | Apparatus for and Method of Processing Biological Samples - The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like. | 02-12-2015 |
20150044759 | BIOLOGICAL SENSING STRUCTURES - A biological sensing structure includes a mesa integrally connected a portion of a substrate, wherein the mesa has a top surface and a sidewall surface adjacent to the top surface. The biological sensing structure includes a first light reflecting layer over the top surface and the sidewall surface of the mesa. The biological sensing structure includes a filling material surrounding the mesa, wherein the mesa protrudes from the filling material. The biological sensing structure includes a stop layer over the filling material and a portion of the first light reflecting layer. The biological sensing structure includes a second light reflecting layer over a portion of the stop layer and a portion of the top surface of the mesa. The biological sensing structure includes an opening in the second light reflecting layer to partially expose the top surface of the mesa. | 02-12-2015 |
20150044760 | FLUID MIXING AND DELIVERY IN MICROFLUIDIC SYSTEMS - The specification generally discloses systems and methods for mixing and delivering fluids in microfluidic systems. The fluids can contain, in some embodiments reagents that can participate in one or more chemical or biological reactions. Some embodiments relate to systems and methods employing one or more vent valves to controllably flow and/or mix portions of fluid within the microfluidic system. Advantageously, fluid control such as a sequence of fluid flow and/or a change in flow rate, can be achieved by opening and closing one or more vent valves and by applying a single source of fluid flow (e.g., a vacuum) operated at a substantially constant pressure. This can simplify the operation and use of the device by an intended user. | 02-12-2015 |
20150050720 | DEVICE FOR DETECTION OF ANALYTES IN AFFINITY BIOASSAYS - A device for detection of analytes in affinity bioassays comprising at least one transparent wall portion, at least one test recess adapted to receive at least one test strip and arranged such that said test strip is visible through the transparent wall portion, at least one cavity adapted to receive a container containing a fluid sample, said cavity being in fluid communication with said at least one test recess, and piercing means arranged in correspondence with said at least one cavity, such that upon insertion of a container in the cavity the container is pierced and at least a portion of the sample is released from the container and reaches the test recess. The invention further relates to the use of such a device for detection of analytes of a nucleic acid amplification reaction and to a detection kit comprising such a device and a test strip. | 02-19-2015 |
20150050721 | CHIP FOR ANALYSIS OF TARGET SUBSTANCE - The present invention provides a chip for analysis of a target substance that is compact and allows analysis of a target substance with less time and effort. The chip for analysis of a target substance includes a first flexible substrate | 02-19-2015 |
20150056687 | LATERAL FLOW DEVICES AND METHODS OF MANUFACTURE AND USE - A lateral flow assay test device providing a structure for the lateral flow assay reactions provides for a continuous flow path of bibulous material provided in separate but contiguous regions of the device in which the bibulous layers are in fluid contact with each other thereby providing flow control of the timing and speed of the assay reaction. Increased flow control results in increasing reliability of use, increasing sophistication of reactions and increases the range of molecules or diagnosis that can be identified. Such flow control can extend processing times and allow users or test givers to manually delay test processing providing enhanced test results. | 02-26-2015 |
20150056688 | Dual Path Immunoassay Device - Systems include test cells with sorbent material in a T-shape and defining a first flow path for a solution and a second flow path for a sample, and a test line or test site with immobilized antigens or antibodies or other ligand binding molecules located at the junction of the T. A housing houses the sorbent material and defines a first hole adjacent an end of the first flow path for receiving the solution and a second hole adjacent an end of the second flow path for receiving the sample. | 02-26-2015 |
20150064774 | MICROFLUIDIC APPARATUS - A microfluidic apparatus mounted on a rotation driving unit for inducing a fluid to flow due to a centrifugal force includes: a target chamber that houses a first fluid; a first chamber that houses a second fluid and is disposed closer to a rotation center of the microfluidic apparatus in a radius direction than the target chamber, the first chamber being connected to the target chamber by a first channel; a first valve that prevents a flow of the second fluid through the first channel; and a second chamber disposed closer to the rotation center in the radius direction than the target chamber and connected to the target chamber by a second channel, wherein the first fluid is transported to the second chamber by supplying the second fluid to the target chamber by the centrifugal force. | 03-05-2015 |
20150064775 | RANDOM ACCESS SYSTEM AND METHOD FOR POLYMERASE CHAIN REACTION TESTING - A random access, high-throughput system and method for preparing a biological sample for polymerase chain reaction (PCR) testing are disclosed. The system includes a nucleic acid isolation/purification apparatus and a PCR apparatus. The nucleic acid isolation/purification apparatus magnetically captures nucleic acid (NA) solids from the biological sample and then suspends the NA in elution buffer solution. The PCR testing apparatus provides multiple cycles of the denaturing, annealing, and elongating thermal cycles. More particularly, the PCR testing apparatus includes a multi-vessel thermal cycler array that has a plurality of single-vessel thermal cyclers that is each individually-thermally-controllable so that adjacent single-vessel thermal cyclers can be heated or cooled to different temperatures corresponding to the different thermal cycles of the respective PCR testing process. | 03-05-2015 |
20150072403 | PORTABLE APPARATUS FOR IMPROVED SAMPLE ANALYSIS - The present invention is an improved apparatus for sample analysis. The apparatus employs an assay component containing a membrane having one or a plurality of analyte-specific binding agents attached thereto, a means for absorbing liquid, and a piston means for drawing analytes through said membrane into said means for absorbing liquid. The apparatus is configured to be portable and provide a detector for detecting binding of an analyte to an analyte-specific binding agent, a plurality of data acquisition components, and a computer for integrating, analyzing and storing the detected analyte specific binding and acquired data. | 03-12-2015 |
20150079665 | MICROFLUIDIC CHIPS WITH OPTICALLY TRANSPARENT GLUE COATING AND A METHOD OF MANUFACTURING MICROFLUIDIC CHIPS WITH OPTICALLY TRANSPARENT GLUE COATING FOR A MICROFLUIDIC DEVICE - A microfluidic chip for a microfluidic system includes a PDMS substrate having a first thickness, at least one microfluidic pathway in the substrate, a coating along the microfluidic pathway, and a glass layer having a second thickness on the substrate and above the microfluidic pathway, wherein the coating contains an optically transparent material, and the first thickness is greater than the second thickness. The coating includes cyanoacrylates, an UV curable epoxy adhesive, a gel epoxy or epoxy under trade name of EPO-TEK 0G175, MasterBond EP30LV-1 or Locite 0151. | 03-19-2015 |
20150079666 | THERMOCYCLING SYSTEM, COMPOSITION, AND MICROFABRICATION METHOD - A system and method of manufacture for the system, comprising a set of heater-sensor dies, each heater-sensor die comprising an assembly including a first insulating layer, a heating region comprising an adhesion material layer coupled to the first insulating layer and a noble material layer, and a second insulating layer coupled to the heating region and to the first insulating layer through a pattern of voids in the heating region, wherein the pattern of voids in heating region defines a coarse pattern associated with a heating element of the heating region and a fine pattern, integrated into the coarse pattern and associated with a sensing element of the heating region; an electronics substrate configured to couple heating elements and sensing elements of the set of heater-sensor dies to a controller; and a set of elastic elements configured to bias each of the set of heater-sensor dies against a detection chamber. | 03-19-2015 |
20150079667 | THERMOCYCLING SYSTEM AND MANUFACTURING METHOD - A system for thermocycling biological samples within detection chambers comprising: a set of heater-sensor dies, each heater-sensor die comprising a heating surface configured to interface with a detection chamber and a second surface, inferior to the heating surface, including a first connection point; an electronics substrate, comprising a first substrate surface coupled to the second surface of each heater-sensor die, an aperture providing access through the electronics substrate to at least one heater-sensor die, and a second substrate surface inferior to the first substrate surface, wherein the electronics substrate comprises a set of substrate connection points at least at one of the first substrate surface, an aperture surface defined within the aperture, and the second substrate surface, and wherein the electronics substrate is configured to couple heating elements and sensing elements of the set of heater-sensor dies to a controller; and a set of wire bonds, including a wire bond coupled between the first connection point of at least one of the set of heater-sensor dies and one of the set of substrate connection points. | 03-19-2015 |
20150079668 | FLUIDIC DEVICE, TESTING DEVICE, AND METHOD FOR FABRICATING FLUIDIC DEVICE - Provided is a fluidic device including: a support member; a porous layer; and an adhesive layer bonding the support member and the porous layer with each other. A partition wall made of a thermoplastic material is formed in the porous layer. The support member has a heatproof temperature that is higher than a melting start temperature of the thermoplastic material. | 03-19-2015 |
20150093815 | MICROFLUIDIC CARTRIDGE DEVICES AND METHODS OF USE AND ASSEMBLY - Methods and systems for controlling fluid flow through a cartridge for use in an assay of a fluid sample for detection of a target analyte. In one aspect, the cartridge includes a microfluidic device having a network of microfluidic channels, one or more reservoirs and one or more actuatable valves for controlling fluid flow from the reservoirs through the microfluidic channels. In some embodiments, the valve includes a deformable membrane movable between a closed configuration in which the membrane seals against the microfluidic device inhibiting fluid flow and an open configuration moved away from the microfluidic device to allow fluid flow through the microfluidic channels. Other valve embodiments utilize frangible membranes or user-removable membrane. | 04-02-2015 |
20150093816 | Compact Fluid Analysis Device and Method to Fabricate - The present disclosure relates to a device for analyzing a fluid sample. In one aspect, the device includes a fluidic substrate that comprises a micro-fluidic component embedded in the fluidic substrate configured to propagate a fluid sample via capillary force through the device and a means for providing a fluid sample connected to the micro-fluidic component. The device also includes a lid attached to the fluidic substrate at least partly covering the fluidic substrate and at least partly closing the micro-fluidic component. The fluidic substrate may be a silicon fluidic substrate and the lid may be a CMOS chip. In another aspect, embodiments of the present disclosure relate to a method for fabricating such a device, and the method may include providing a fluidic substrate, providing a lid, and attaching, through a CMOS compatible bonding process, the fluidic substrate to the lid to close the fluidic substrate at least partly. | 04-02-2015 |
20150099291 | SELF-CONTAINED BIOLOGICAL ANALYSIS - Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include nucleic acid amplification and detection and immuno-PCR. | 04-09-2015 |
20150104859 | Rapid Thermal Cycling for PCR Reactions using Enclosed Reaction Vessels and Linear Motion - A system for fast, accurate and inexpensive thermal cycling is disclosed including a set of thermally conductive plates that are maintained in a fixed spatial relationship to each other, and separated from each other by a thermal insulating space. A hole having a size approximately equal to a size of a desired test sample is formed through the set of thermally conductive plates. The test sample is placed in the hole and moved back and forth between the different temperature plates, to the desired temperature locations for desired time periods, in a pattern that is determined by the user, and repeated as many times as needed for the specific process. | 04-16-2015 |
20150104860 | Smartphone Biosensor - A mobile computing device that includes an image sensor may be used to detect the result of a biomolecular assay. The biomolecular assay may be performed in an optical assay medium that provides an optical output in response to light from a light source, with the optical output indicating result. A wavelength-dispersive element may be used to disperse the optical output into spatially-separated wavelength components. The mobile computing device may be positioned relative to the wavelength-dispersive element such that different wavelength components are received at different locations on the image sensor. With the mobile computing device positioned in this way, the image sensor may be used to obtain one or more images that include the separated wavelength components of the optical output. A wavelength spectrum of the optical output may be determined from the one or more images, and the result may be determined from the wavelength spectrum. | 04-16-2015 |
20150111287 | PORTABLE DEVICE FOR DETECTING MOLECULE(S) - An optical assembly | 04-23-2015 |
20150111288 | SYSTEM AND METHOD INCLUDING ANALYTICAL UNITS - Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples. | 04-23-2015 |
20150111289 | FLUID INTERFACE CARTRIDGE FOR A MICROFLUIDIC CHIP - An interface cartridge for a microfluidic chip, with microfluidic process channels and fluidic connection holes at opposed ends of the process channels, provides ancillary fluid structure, including fluid flow channels and input and/or waste wells, which mix and/or convey reaction fluids to the fluidic connection holes and into the process channels of the microfluidic chip. | 04-23-2015 |
20150111290 | OPTICAL DETECTOR - Disclosed herein is an optical detector at least including: a first substrate in which a plurality of wells are formed; a second substrate in which a heating section is provided to heat the wells; a third substrate in which a plurality of photoirradiation sections are provided in alignment with the wells; and a fourth substrate in which a plurality of photodetection sections are provided in alignment with the wells. | 04-23-2015 |
20150118738 | MICROFLUIDIC CHIP FEATURES FOR OPTICAL AND THERMAL ISOLATION - A microfluidic chip includes microfluidic channels, elements for thermally and optically isolating the microfluidic channels, and elements for enhancing the detection of optical signal emitted from the microfluidic channels. The thermal and optical isolation elements may comprise barrier channels interposed between adjacently-arranged pairs of microfluidic channels for preventing thermal and optical cross-talk between the adjacent microfluidic channels. The isolation element may alternatively comprise reflective film embedded in the microfluidic chip between the adjacent microfluidic channels. The signal enhancement elements comprise structures disposed adjacent to the microfluidic channels that reflect light passing through or emitted from the microfluidic channel in a direction toward a detector. The structures may comprise channels or a faceted surface that redirects the light by total internal reflection or reflective film material embedded in the microfluidic chip. | 04-30-2015 |
20150118739 | MICRO FLOW-CHANNEL CHIP, METHOD FOR MANUFACTURING THE SAME, AND DEVICE FOR ANALYSIS - The present invention provides a micro flow-channel chip formed of two or more parts, wherein a first part has a through hole that forms a flow-channel connection portion, a second part has a projecting portion, the projecting portion of the second part is inserted into the through hole of the first part, thereby a volume of the flow-channel connection portion is reduced, and furthermore, both of the first part and the second part are molded with a die. | 04-30-2015 |
20150118740 | SELF-CONTAINED CARTRIDGE AND METHODS FOR INTEGRATED BIOCHEMICAL ASSAY AT THE POINT-OF-CARE - Provided is a sample processing cartridge. The sample processing cartridge can include a housing and a channel disposed in the housing. The housing can include a sample inlet for receiving at least one biological sample. The channel can in fluidic communication with the sample inlet and can be defined by an upper surface and a lower surface. The upper surface can include a hydrophilic portion and a hydrophobic portion. The lower surface can include a hydrophilic portion and a hydrophobic portion. The hydrophilic and hydrophobic portions of the upper and lower surfaces of the channel can be configured to isolate at least one aqueous reagent. | 04-30-2015 |
20150118741 | SYSTEMS AND METHODS FOR PROVIDING MICROFLUIDIC DEVICES - A microfluidic valve system is disclosed that includes a matrix, a hydrophilic acceptor region a hydrophilic transfer region, and a hydrophobic gap between the acceptor region and the transfer region. | 04-30-2015 |
20150118742 | APPARATUS FOR EX VIVO MICROFLUIDIC ANALYSIS OF BIOLOGIC SAMPLES - Systems and methods for culturing and monitoring, ex vivo, pharmacologic and metabolic response in a biological sample, including receiving at a fluidic apparatus the biological sample retrieved from the patient, retaining the biological sample within a channel of the fluidic apparatus, providing for the culture of the biological sample within the channel of the fluidic apparatus, flowing a fluid past the biological sample, retrieving and analyzing the fluid to determine a pharmacologic and/or metabolic response of the sample. | 04-30-2015 |
20150125938 | LIQUID SAMPLE MEASURING SYSTEM AND MEASURING DEVICE - An liquid sample measuring system includes a measuring device including a measuring section which measures biological information from liquid sample of a living subject within a housing in which a biosensor, on which the liquid sample of the biological body is deposited, is detachably mounted and a movement measuring section which measures movement information of the housing within the housing, and an administrating device including a movement determining section which determines whether or not a degree of the movement of the housing is within an allowable range by analyzing the movement information received from the measuring device. | 05-07-2015 |
20150125939 | REAGENT DELIVERY SYSTEM - A delivery apparatus for selectively delivering one or more liquid reagents into a reaction or test chamber ( | 05-07-2015 |
20150125940 | CONTAINER CLEANING DEVICE, DISCHARGE MEMBER FOR CONTAINER CLEANING DEVICE, AND ANALYZER - Disclosed is a container cleaning device which cleans a container for holding a sample. The device comprises: a container holding section for holding the container which has a substantially spherical inner bottom; a discharge section which is inserted into the container; and a cleaning nozzle which discharges a cleaning liquid into the container. The discharge section comprises an aperture at the bottom, and comprises a discharge flow path which is connected to the aperture and discharges the sample or the cleaning liquid in the container, and the discharge section is insertable to the deepest part of the container, and the bottom part of the discharge section conforms to the shape of the substantially spherical inner bottom of the container. | 05-07-2015 |
20150125941 | DETECTION SYSTEM OF TEST SUBSTANCE - A detection system of a test substance, includes: a magnetic body that is modified by a first substance capable of being bound to the test substance at a first binding site of the test substance; a marker that is modified by a second substance capable of being bound to the test substance at a second binding site of the test substance, the second coupling site being a different site from the first binding site; a development medium that has a flow passage into which liquid, in which the magnetic body and the marker is dispersed, flows due to a capillary phenomenon, the development medium having a detection position for detecting the marker in a portion of the flow passage, the development medium having light transparency at least in the detection position; and a magnetic force generating portion that is equipped in the development medium. | 05-07-2015 |
20150132835 | DEVICES, SYSTEMS, AND METHODS FOR DIAGNOSTIC TESTING - The present disclosure relates to devices, systems, and methods for performing diagnostic tests. The disclosed diagnostic devices are capable of performing analytic tests and communicating with a portable multifunctional device (PMD) or other computing device. Through input and manipulation of materials within the diagnostic device, a large range of tests may be performed. For example, alteration or customization of chemical components of the diagnostic device may enable many analytic applications to be provided. These analytic tests may include, but are not limited to, sensing or quantification of chemicals from sample input, whether gaseous, liquid, or otherwise, sensing or quantification of analytes, antibodies, or antigens, sensing or quantification of genetic material, or other substances. | 05-14-2015 |
20150132836 | Method And System For Detecting Lymphosarcoma in Cats Using Biomarkers - The invention provides a method and system for developing and using diagnoses of lymphosarcoma in feline subjects using Thymidine kinase (TK) and haptoglobin (HP) as biomarkers. The invention provides a method for obtaining the level of each biomarker and computing an index for a feline subject. The invention provides predefined index ranges to which the index value may be matched in order to determine whether the subject has a high probability of being affected by lymphosarcoma, even when the subject shows apparent symptoms that may be common with inflammatory bowel disease. | 05-14-2015 |
20150140645 | Microfluidic Analysis System - A thermal cycling device ( | 05-21-2015 |
20150140646 | SYSTEMS AND METHODS FOR DETECTION AND QUANTIFICATION OF ANALYTES - Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. In some embodiments, the reader component communicates with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample. | 05-21-2015 |
20150298119 | MICROFLUIDIC CARTRIDGE FOR PROCESSING AND DETECTING NUCLEIC ACIDS - A microfluidic cartridge, configured to facilitate processing and detection of nucleic acids, comprising: a top layer comprising a set of cartridge-aligning indentations, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of Detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a Detection chamber, comprises a turnabout portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region. | 10-22-2015 |
20150301039 | Biological Sensor and a Method of the Production of Biological Sensor - The invention is related to the field of biotechnology, specifically to the investigation of biomolecular interactions and sensing of biomolecules using a surface plasmon resonance. The biological sensor and a method of its production based on the thin films of graphene, graphene oxide, or single-walled or multi-walled carbon nanotubes are described. | 10-22-2015 |
20150307927 | APPARATUS FOR CENTRIFUGE MOUNTABLE MANIFOLD FOR PROCESSING FLUIDIC ASSAYS - The disclosure generally relates to a method and apparatus for centrifuge mountable manifold for processing fluid assays. In one embodiment, the disclosure relates to a method for automatically running an assay with a radially configured flowrotor by: introducing a sample fluid to an inlet of the flowrotor; rotating the flowrotor in a first direction to provide a first radial acceleration to move the sample fluid from the inlet to a first reaction chamber, the first reaction chamber having one or more reactants; retaining the assay at the first reaction chamber for a first duration by maintain rotation at a first angular velocity to induce a reaction with the reactant and to provide a reacted assay; rapidly accelerating the flowrotor at a negative angular acceleration relative to the first angular velocity until the angular velocity is reversed thereby moving the reacted assay from the first reaction chamber to a second reaction chamber having an inlet and an outlet and containing a second reactant or reactants and retaining the assay at the second chamber for a second duration by continuing the second angular velocity to thereby induce a reaction with the second reactant. | 10-29-2015 |
20150308958 | OPTICAL INTERROGATION DEVICE - An interrogation device for detecting luminescent light produced by analytes in a sample excited by multiple excitation light beams each having individual spectral contents, comprising a plurality of light sources each generating an excitation light beam; at least one detector for detecting the luminescent light produced by the sample; and an optical assembly defining distinct and fixed excitation light paths for each of the excitation light beams from the light sources to a common excitation site on the sample and defining a shared luminescence light path for the luminescent light from the excitation site on sample to the at least one detector, the excitation light paths and the luminescence light path being on a same side of the sample, the optical assembly including sample-side optics projecting the excitation light towards the sample and collecting luminescent light from the sample. | 10-29-2015 |
20150308959 | BASE SEQUENCE ANALYSIS APPARATUS - There is provided a base sequence analysis method including a nucleic acid amplification procedure of obtaining an amplification product by a nucleic acid amplification reaction, a turbidity measurement procedure of measuring turbidity of a reaction solution of the nucleic acid amplification reaction, and a melting curve analysis procedure of performing melting curve analysis of a probe nucleic acid chain and the amplification product at a reaction site of the nucleic acid amplification reaction. | 10-29-2015 |
20150309058 | SEQUENCE SCHEDULING AND SAMPLE DISTRIBUTION TECHNIQUES - A technique is disclosed for sample management for use in conjunction with sequencing devices that sequence biological samples, e.g., DNA and RNA. A sequencing device or a network of sequencing devices may be scheduled according to the characteristics of the samples in queue and the capabilities and availability of sequencing devices. Biological samples may be automatically queued and loaded via a sample distribution system. A sample distribution system may be used to reduce operator intervention. | 10-29-2015 |
20150322488 | Instrument for Monitoring Polymerase Chain Reaction of DNA - An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes. | 11-12-2015 |
20150323527 | Methods and Devices for Using Mucolytic Agents Including N-Acetyl Cysteine (NAC) - Devices and methods incorporate mucolytic agents into a point-of-care testing device. The sample is loaded, and then the sample travels until it encounters one or more lysis agents and/or mucolytic agents. The mucolytic agent is preferably pre-loaded onto the collection device. In a preferred embodiment, the mucolytic agent is localized between the sample application zone and the conjugate zone. In embodiments with a sample compressor, one or more mucolytic agents may be pre-loaded and dried on the sample compressor, the sample collector, in various locations on the test strip, or in the running buffer. | 11-12-2015 |
20150330878 | SAMPLE ANALYZER AND A SAMPLE ANALYZING METHOD - Herewith disclosed is a sample analyzer comprising: a measurement section configured to perform a measurement on a sample and generate a measurement value according to the concentration of an analyte in the sample; a memory storing a calibration curve; an analysis section; an output section; and an instruction receiver. When the instruction receiver receives an instruction to perform a diluting measurement on a calibration sample, the measurement section dilutes the calibration sample by a predetermined ratio and performs a measurement on the diluted calibration sample, and the analysis section determines the concentration of the analyte in the diluted calibration sample by applying a measurement value obtained from the diluted calibration sample to the calibration curve. Information generated based on the determined concentration and the known concentration is output. | 11-19-2015 |
20150330971 | SYSTEMS AND METHODS FOR DETECTING A BIOLOGICAL CONDITION - The present invention provides self-contained systems, apparatus and methods for determining a chemical state, the system includes a stationary cartridge for performing the assay therein, the cartridge adapted to house at least one reagent adapted to react with a sample; and at least one reporter functionality adapted to report a reaction of the at least one reagent with the sample to report a result of the assay, a mechanical controller including a first urging means adapted to apply a force externally onto the cartridge to release the at least one reagent; and at least one second urging means adapted to apply a removable force to induce fluidic movement in a first direction in the cartridge and upon removal of the force causing fluidic movement in an opposite direction to the first direction, an optical reader adapted to detect the reaction and a processor adapted to receive data from the optical reader and to process the data to determine said chemical state. | 11-19-2015 |
20150338346 | Method For Highly Sensitive Detection of Biomarkers for Diagnostics - This disclosure provides, among other things, a nanosensor comprising a substrate and one or a plurality of pillars extending from a surface of the substrate, where the pillars comprise a metallic dot structure, a metal disc, and a metallic back plane. The nanosensor comprises a molecular adhesion layer that covers at least a part of the metallic dot structure, the metal disc, and/or the metallic back plane and a capture agent bound to the molecular adhesion layer. The nanosensor amplifies a light signal from an analyte, when the analyte is specifically bound to the capture agent. | 11-26-2015 |
20150346105 | DEVICE AND APPARATUS - There is provided a device ( | 12-03-2015 |
20150346212 | SYSTEM FOR DETECTION OF A COMPONENT IN A FLUID - The invention provides a system for detection of a component in a fluid, the system comprising a detector configured to detect presence of the component in the fluid, the detector being partially covered by a fluid-protective seal wherein only a specimen sampling region is uncovered by the fluid-protective seal. The system can be fully submerged in water and still retain its functionality. | 12-03-2015 |
20150360227 | Pinch Flow Regulator - A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. The cover fluid inlet is in fluidic communication with the base fluid outlet between the upper cavity and the lower cavity, and the cover fluid outlet provides external access from the upper cavity. A diaphragm is disposed between the housing base and the housing cover. A pinch plate is disposed in the lower cavity and comprises a pinch point disposed opposite the pinch structure. A pinch tube is in fluidic communication between the base fluid inlet and the base fluid outlet in the lower cavity. | 12-17-2015 |
20150367314 | ANALYSIS DEVICE, GENETIC ANALYSIS METHOD, ANALYSIS RECEPTACLE, AND CONTROL METHOD FOR FUZZY CONTROL - With this analysis device, air that has been heated by a heater is blown by a fan in the direction of an analysis receptacle rotary driver and an analysis receptacle that is rotationally driven by the analysis receptacle rotary driver. The analysis receptacle rotary driver rotates the analysis receptacle within the analysis chamber in the same direction as the direction of air flow produced by the fan. | 12-24-2015 |
20160003814 | BIOANALYSIS DEVICE AND BIOMOLECULE ANALYZER - Paramagnetic fine particles of 1 micron or less used under a strong magnetic field were shown to form beads-like aggregates along the magnetic flux, and become irregularly shaped as such a mass of particles combines with a flat particle layer. This phenomenon becomes a factor that degrades the quality of quantification in bioanalysis. By confining a solution of microscopic magnetic fine particles between flat substrates of high wettability as thin a vertical thickness as possible and attracting the magnetic fine particles under a magnetic field applied from the side of one of the flat substrates, the magnetic fine particles can be evenly immobilized in the form of a film on the substrate surface in a dispersion state, and the quality of the biomolecule quantification can be improved. | 01-07-2016 |
20160010149 | INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS | 01-14-2016 |
20160017404 | SYSTEMS AND METHODS FOR MONITORING THE AMPLIFICATION AND DISSOCIATION BEHAVIOR OF DNA MOLECULES - The present invention relates to systems and methods for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules. | 01-21-2016 |
20160018313 | Method for Hematology Analysis - A method whereby one or more fluorescent dyes are used to bind and stain nucleic acids in certain blood cells, such as, for example, white blood cells, nucleated red blood cells, and reticulocytes, and to induce fluorescent emissions upon excitation of photons from a given source of light, such as, for example, a laser, at an appropriate wavelength. More particularly, this invention provides a method whereby a fluorescent trigger is used in a data collection step for collecting events that emit strong fluorescence, in order to separate white blood cells and nucleated red blood cells from red blood cells and platelets without the need for using a lysing agent. | 01-21-2016 |
20160025722 | METHOD FOR PRETREATING SPECIMEN AND METHOD FOR ASSAYING BIOLOGICAL SUBSTANCE - This invention relates to an assay device and a method for pretreating a specimen containing a biologically-relevant substance and then assaying the biologically-relevant substance. Biologically-relevant substances used in the assay method include microorganisms, cells, viruses, nucleic acids, polysaccharides, proteins (including antigens, antibodies, chromoproteins, and enzymes), peptides, nucleic acids, and small molecules. This pretreatment method removes contaminants from the biologically-relevant substances using supports such as magnetic particles, gels, resins, membranes, and solid-phased reagents. Therefore, in the assay method the following steps are generally carried out (i) a pretreatment step of treating a specimen using one or more first supports and then one or more second supports, and (ii) the assay step carried out after the pretreatment step. In particular, the pretreatment method reduces false positives and false negatives in the assay. | 01-28-2016 |
20160032361 | CENTRIFUGE CONFIGURATIONS - Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample. | 02-04-2016 |
20160033448 | ACTIVE CHEMICALLY-SENSITIVE SENSORS WITH RESET SWITCH - Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions. | 02-04-2016 |
20160033486 | SAMPLE ANALYSIS DEVICE AND CAPTURING METHOD FOR EXOSOMES - A recessed portion and a protruding portion arranged periodically are formed on a base portion. In the recessed portion, an antibody that binds to an antigen existing on a surface of each exosome to be detected is immobilized and then caused to bind to the exosomes. The width of the protruding portion is smaller than the average particle diameter of the exosomes. | 02-04-2016 |
20160033487 | DISPENSING DEVICE, IMMUNOASSAY ANALYZER AND METHOD THEREOF - Dispensing devices and methods are provided. The dispensing devices can include a first dispensing station arranged on a periphery of a holding unit for dispensing a first reaction component, and a second dispensing station arranged on the holding unit for dispensing a second reaction component. The first reaction component and the second reaction component are dispensed. Operations on the holding unit are not affected by dispensing of the first reaction component, improving test efficiency. Because reaction containers do not need to be transferred out of the holding unit to dispense the second reaction component, test processes are simplified, In addition, the dispensing unit is not restricted to being arranged around the holding unit, and the first dispensing unit and second dispensing unit are arranged separately, so restriction of and interference with the dispensing units are avoided. | 02-04-2016 |
20160040213 | SYSTEMS AND METHODS FOR DETECTING THE PRESENCE OF A SELECTED VOLUME OF MATERIAL IN A SAMPLE PROCESSING DEVICE - Systems and methods for processing sample processing devices. The system can include a sample processing device comprising a detection chamber, a motor configured to rotate the sample processing device about an axis of rotation, and an optical module operatively positioned relative to the sample processing device and configured to determine whether a selected volume of material is present in the detection chamber of the sample processing device. The method can include rotating the sample processing device about an axis of rotation, and determining whether a selected volume of material is present in the detection chamber, while rotating the sample processing device. In some embodiments, determining whether a selected volume of material is present can be performed by optically interrogating the detection chamber for an optical property of the material. | 02-11-2016 |
20160047832 | SYSTEM FOR OPERATING A SYSTEM FOR THE INTEGRATED AND AUTOMATED ANALYSIS OF DNA OR PROTEIN - A system for the integrated and automated analysis of DNA or protein, including a single-use cartridge, with an analysis device having a control device, and devices for capturing and processing signals, the control device carrying out a completely automatic process and evaluation of molecular diagnostic analysis via single-use cartridges (Lab-on-a-Chip). Controlling of an analysis process, which occurs in the cartridge, involves the subsequent displacement and thermostatisation of liquids with a first device, and with a second device the signals which are obtained during the analysis are processed. The first and the second devices are synchronized in such a manner that the analysis process of the sample can be carried out in a totally integrated manner thus producing an immediate result. | 02-18-2016 |
20160054315 | NANOELECTRONIC-ENZYME LINKED IMMUNOSORBENT ASSAY SYSTEM AND METHOD - The present invention relates to a device and method for determining the presence of a specific compound in solution. The device includes a nanosensor having an electrically conducting pathway between at least a first and second contact. The device also includes a first receptor, suitable for binding a specific compound in the solution, attached to the nanosensor, and a second receptor also suitable for binding the specific compound while the specific compound is bound to the first receptor. The second receptor is attached to an enzyme added to the solution. When the solution having the second receptor is added to the device, and a second compound that is a substrate for the enzyme is subsequently added to the solution, a measured difference in an electrical property in the device before and after the application of the second compound is indicative of the presence of the specific compound in the solution. | 02-25-2016 |
20160059233 | MICROFLUIDIC CHIPS WITH OPTICALLY TRANSPARENT GLUE COATING AND A METHOD OF MANUFACTURING MICROFLUIDIC CHIPS WITH OPTICALLY TRANSPARENT GLUE COATING FOR A MICROFLUIDIC DEVICE - A microfluidic chip for a microfluidic system includes a PDMS substrate having a first thickness, at least one microfluidic pathway in the substrate, a coating along the microfluidic pathway, and a glass layer having a second thickness on the substrate and above the microfluidic pathway, wherein the coating contains an optically transparent material, and the first thickness is greater than the second thickness. The coating includes cyanoacrylates, an UV curable epoxy adhesive, a gel epoxy or epoxy under trade name of EPO-TEK OG175, MasterBond EP30LV-1 or Locite 0151. | 03-03-2016 |
20160059235 | Reagent-Loaded Pencils and Methods - A reagent-loaded pencil, method of preparing a reagent-loaded pencil, and a system that uses reagent-loaded pencils are disclosed. The reagent-loaded pencil or pencils include a matrix of at least a soluble carrier, a hardening substance, and a reagent. The pencils also include a sheath disposed around at least a portion of the matrix. The soluble carrier may include poly(ethylene glycol) methyl ether. The hardening substance may leave a visible mark when abraded against a writing surface. One example of such a substance includes graphite. The sheath may be the outside of a wooden pencil or a mechanical pencil. A system that uses reagent-loaded pencils or compressed matrices may include a computer-controlled plotter that holds and plots with at least one of the reagent-loaded pencils or matrices. | 03-03-2016 |
20160059237 | THERMAL CYCLING - A processing apparatus includes a carrier receiving region configured to receive a sample carrier with at least one channel that carries at least one sample. The apparatus further includes a thermal control device configured to thermal cycle the sample carrier when the sample carrier is installed in the carrier receiving region, thereby thermal cycling the sample carried therein. The apparatus further includes a thermal control system configured to control the temperature control device based on a predetermined set of target sample temperatures and a temperature map, which maps the predetermined set of target sample temperatures to a set of temperatures of the temperature control device. The set of temperatures of the temperature control device is different from the predetermined set of target sample temperatures, and the set of temperatures of the temperature control device thermal cycle the sample carrier with the temperatures of the predetermined set of target sample temperatures. | 03-03-2016 |
20160061822 | METHOD AND APPARATUS FOR BACTERIAL MONITORING - A system for detecting target elements such as bacteria in a host analyte, comprising a substrate with an ordered array of wells having diameters to fit the size of the targets. The substrate may be a periodic macro-PSi array structure (MPSiAS) illuminated with a broadband source. The reflected light spectrum diffracted from the substrate is optically analyzed to provide the effective optical depth of the wells. Fast Fourier Transform analysis may be used for the optical analysis. Entry of target elements into wells is detected by the change in the effective optical depths of the wells. Micro-organisms as large as bacteria and viruses having dimensions comparable with the wavelength of the illumination can thus be detected. Wells with an inner section impenetrable by the target cells enables compensation for environmental changes. The detection may be performed in real time, such that production line bacterial monitoring may be achieved. | 03-03-2016 |
20160074862 | CHEMICAL VAPOR DEPOSITION COATING FOR LABORATORY WARE - An article of laboratory ware is disclosed comprising a body and a chemical vapor deposition coating. The body is made of thermoplastic material (“thermoplastic” includes glass or polymer resins) having a support surface. The chemical vapor deposition coating is applied directly or indirectly on the support surface. The coating defines a contact surface having a wetting tension of from 20 to 75 dyne/cm, for example. Useful wetting tensions beyond this range are contemplated as well. | 03-17-2016 |
20160077006 | OPTICAL DETECTOR - Disclosed herein is an optical detector at least including: a first substrate in which a plurality of wells are formed; a second substrate in which a heating section is provided to heat the wells; a third substrate in which a plurality of photoirradiation sections are provided in alignment with the wells; and a fourth substrate in which a plurality of photodetection sections are provided in alignment with the wells. | 03-17-2016 |
20160077086 | Systems and Methods of Sample Processing and Fluid Control in a Fluidic System - This invention is in the field of medical devices. Specifically, the present invention provides portable medical devices that allow real-time detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications. | 03-17-2016 |
20160084762 | Methods and Compositions for Highly Sensitive Detection of Molecules - Disclosed are methods, kits, and compositions for the highly sensitive detection of molecules. The methods, kits, and compositions are useful in determining concentrations of molecules in samples to levels of 1 femtomolar, 1 attomolar, or lower. The methods, kits, and compositions also allow the determination of concentration over a wide range, e.g., 7-log range, without need for sample dilution. | 03-24-2016 |
20160084831 | FLOW THROUGH TESTING SYSTEM WITH PRESSURE INDICATOR - A device for performing immunoassays on analytes is provided. The device includes an immunosorbent membrane, an absorbent material, a piston component located below said absorbent material to draw analytes in a sample through the immunosorbent membrane into the absorbent material, and discrete groups of pressure-sensitive microcapsules located on the immunosorbent membrane. | 03-24-2016 |
20160091479 | METHOD AND DEVICES FOR ELECTRONIC SENSING - The presence of a detectable entity within a detection volume of a microfabricated elastomeric structure is sensed through a change in the electrical or magnetic environment of the detection volume. In embodiments utilizing electronic detection, an electric field is applied to the detection volume and a change in impedance, current, or combined impedance and current due to the presence of the detectable entity is measured. In embodiments utilizing magnetic detection, the magnetic properties of a magnetized detected entity alter the magnetic field of the detection volume. This changed magnetic field induces a current which can reveal the detectable entity. The change in resistance of a magnetoresistive element may also reveal the passage of a magnetized detectable entity. | 03-31-2016 |
20160091518 | SYSTEMS AND METHODS FOR DETECTION AND QUANTIFICATION OF ANALYTES - Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. In some embodiments, the reader component communicates with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample. | 03-31-2016 |
20160102354 | Systems For Biomarker Detection - Biomarker associated with Alzheimer's Disease (AD) and/or Mild Cognitive Impairment (MCI), where the biomarker is an intracellular or circulating microRNA and/or target protein thereof, and use thereof in methods for determining, diagnosing, monitoring AD, mild AD, moderate Ad, severe AD, MCI, early MCI, late MCI, and the like, as well as in methods for selecting candidate therapeutic compounds for treating same. | 04-14-2016 |
20160103094 | ELECTRO-DIFFUSION ENHANCED BIO-MOLECULE CHARGE DETECTION USING ELECTROSTATIC INTERACTION - According to one aspect, the disclosure is directed to an example embodiment in which a circuit-based arrangement includes a circuit-based substrate securing a channel, with an effective width that is not limited by the Debye screening length, along a surface of the substrate. A pair of reservoirs are included in or on the substrate and configured for containing and presenting a sample having bio-molecules for delivery in the channel. A pair of electrodes electrically couple a charge in the sample to enhance ionic current flow therein (e.g., to overcome the electrolyte screening), and a sense electrode is located along the channel for sensing a characteristic of the biological sample by using the electrostatic interaction between the enhanced ionic current flow of the sample and the sense electrode. Actual detection occurs by using a charge-signal processing circuit to process the sensed charge signal and, therefrom, provide an output indicative of a signature for the bio-molecules delivered in the channel. | 04-14-2016 |
20160109055 | Analyzer Assembly Platform - A combination of an analytical apparatus with a platform for mounting such an apparatus comprising at least two units, and a platform on which said apparatus is mounted is disclosed, said platform comprising at least one platform element, and multiple feet for placing the platform elements on a surface. The feet are individually adjustable in height. | 04-21-2016 |
20160109435 | FABRIC-BASE BIOCHEMICAL DETECTING DEVICE AND THE FABRICATING METHOD THEREOF - The present invention provides a fabric-base biochemical detecting device and the fabricating method thereof. The device comprises a fabric-base material, a detecting material, and glue. The fabricating method comprises the following steps of: (S | 04-21-2016 |
20160114319 | MICROFLUIDIC SENSING DEVICE AND SYSTEM - A microfluidic device can include: a channel; a fluid inlet to pass fluid into the channel from a reservoir; a sensor disposed in the channel; and a pump actuator disposed in the channel apart from the sensor to induce fluid flow into the channel. | 04-28-2016 |
20160123976 | MULTIPLE HYBRID IMMUNOASSAY - The invention relates to compositions and methods for the immunoassay of an analyte of interest. The analyte is detected in an immunoassay using three or more antibodies, where in each antibody specifically binds to a different epitope on the analyte. When the analyte of interest in a clinical marker for an acute disease, the detection of the analyte by immunoassay is a diagnosis of the occurrence of the disease. | 05-05-2016 |
20160123977 | Assay Device With Shared Zones - Disclosed is an assay device for determining the presence and/or extent of one or more analytes in liquid sample containing a) first and second assays each comprising a flow-path having a detection zone for immobilising a labelled binding reagent, wherein detection of a labelled binding reagent at one or both detection zones is indicative of the presence and/or extent of one or more analytes; b) a shared reference zone; c) one or more light sources to illuminate the detection zones and the reference zone; d) one or more photodetectors to detect light from the detection zones and the reference zone, which photodetector/s generate a signal, the magnitude of which signal is related to the amount of light detected; and e) signal processing means for processing signals from the photodetector/s. | 05-05-2016 |
20160131645 | Rapid Lateral Flow Assay Method for Low Quantity Liquid or Dry Samples - This invention describes a device for assaying a dry or small volume liquid sample on a lateral flow capillary assay by utilizing a supplementary non-sample aqueous solution that will push the sample up the assay without diluting or significantly diluting the original sample. This invention enables a sample to be assayed rapidly and/or on-site when the sample is in dried or solid form, or when the liquid volume is too small to complete an assay. As an option, the device can display a colored signal when a sufficient amount of liquid sample has been collected or added to the device. The minimal liquid volume requirement of the device significantly reduces the sample collection time to a matter of seconds and resolves situations in which fluids available for collection are not abundant. Therefore, this invention allows the lateral flow test device to be a more rapid, efficient, and versatile apparatus. | 05-12-2016 |
20160139159 | DEVICES, SYSTEMS AND METHODS FOR EVALUATION OF HEMOSTASIS - Provided are devices, systems and methods for evaluation of hemostasis. Also provided are sound focusing assemblies. | 05-19-2016 |
20160144358 | Dynamic Lab on a Chip Based Point-Of-Care Device For Analysis of Pluripotent Stem Cells, Tumor Cells, Drug Metabolites, Immunological Response, Glucose Monitoring, Hospital Based Infectious Diseases, and Drone Delivery Point-of-Care Systems - The invention provides for a novel dynamically configurable point-of-care device for clinical diagnostics and research for analysis of activity associated with pluripotent stem cells, tumor cells, drug metabolites, immunological response, glucose monitoring, cardiovascular diseases, liver cell therapy, cell-cell signaling, epidemic outbreaks, hospital based infectious diseases, pathogens, germ cells, pharmacological compounds, oxidation reduction, microscopy, tomography, flow cytometry, clinical lab testing, and for providing immunoassays, ELISA, electrophoresis, PCR, chromatography, and other laboratory functions. The device comprises a biochemical processing module further comprising a processor and at least one controller, receiving microfluidic elements, sensors, software scripts, an electrically operated interface, flow ports, a user interface, memory, and a communications link, configurable based on analysis of patient data. The invention further provides for multiple-criteria decision analysis for hospital administrators, a wearable device, mobile medical device, molecular electronics configuration, touchscreen recognition, data analytics application, and a drone delivery based point-of-care system. | 05-26-2016 |
20160144366 | NUCLEIC ACID AMPLIFICATION/DETECTION DEVICE AND NUCLEIC ACID INSPECTION DEVICE USING SAME - When taking in outside air with a fan to control the temperature inside a covered portion where a specimen is installed, the internal temperature may change depending on an environment temperature to cause a difference in temperature control. The wind generated by the fan may blow against a reaction container depending on its loading position and it may influence the temperature control over individual specimens and lead to problems with temperature accuracy, temperature rise speed, and temperature drop speed A reaction container avoiding these problems has a covered portion which performs temperature control on the reaction container and in which a cover and a fin cover have a heat insulating structure. A heat source is provided for controlling an internal temperature of an internal covered space. The internal temperature is kept constant and the influence of the environment temperature over the temperature control of the reaction container is minimized. | 05-26-2016 |
20160146735 | FIBER-OPTIC MICRO-PROBES FOR MEASURING ACIDITY LEVEL, TEMPERATURE, AND ANTIGENS - A pH micro-probe, a temperature micro-probe, and an immuno-based micro-probe each include a shaft for transmitting an input light signal and a tip for inserting into a cell or other substance for measuring pH, temperature, and/or antigens. The pH micro-probe and the temperature micro-probe each include a luminescent material positioned on the tip of the micro-probe. The light signal excites the luminescent material so that the luminescent material emits a luminescent light signal. The luminescent light signal has a property value dependent on the pH or temperature being measured and reflects back through the shaft for being measured by a light signal measuring device. The immuno-based micro-probe includes a reflective material that has an effective refractive index dependent on the number of antigen-antibody bonds present on the reflective material. | 05-26-2016 |
20160146793 | KITS, COMPOSITIONS AND METHODS FOR DETECTING A BIOLOGICAL CONDITION - The present invention provides kits, apparatus and methods for determining a biological condition in a mammalian subject, the method includes incubating a specimen from a patient with at least one composition in a kit for a predetermined period of time to form at least one reaction product, when the subject has said biological condition, and receiving an indication of the at least one reaction product responsive to at least one reporter element in the kit thereby providing the indication of the biological condition in the subject. | 05-26-2016 |
20160146803 | HETEROGENOUS ASSAY - The invention relates to a sample testing device for conducting a heterogenous assay, for example an ELISA, in a capillary lumen, using one way flow of sample and wash buffer to move the reaction through the binding, separation and signal measurement steps, thus minimising external intervention. The capillary passage is configured to allow time within different zones for reaction, capture, separation of bound and free fractions, and signal measurement. A combined capture-signal read zone is provided to maximise the capture of signal linked binding member, and signal measurement within the capture zone. | 05-26-2016 |
20160151785 | SYSTEMS AND DEVICES FOR ISOTHERMAL BIOCHEMICAL REACTIONS AND/OR ANALYSIS | 06-02-2016 |
20160153031 | INSPECTION TOOL FOR NUCLEIC ACID CHROMATOGRAPHY | 06-02-2016 |
20160154930 | METHODS FOR IDENTIFICATION OF INDIVIDUALS | 06-02-2016 |
20160158749 | CAPS FOR SAMPLE WELLS AND MICROCARDS FOR BIOLOGICAL MATERIALS - A cover for a biological sample well tray, comprising a cap for sealing a sample well. The cap comprises a well lens for focusing light into the sample well and collecting light from the sample. In another aspect, the cap comprises an elongate portion configured to permit incoming light to pass into the sample well and out of the sample well. Various other aspects comprise a microcard for biological material, and an apparatus for a plurality of sample well strips. A method for testing a biological sample is also provided. | 06-09-2016 |
20160158760 | DISPOSABLE THERMAL IN-VITRO DIAGNOSTIC APPARATUS - A portable, disposable in-vitro diagnostic apparatus is provided. The apparatus includes a body configured to be hand held. The body has a reaction medium supply chamber configured in selective fluid communication with a reaction chamber via a fluid conveying channel. The reaction chamber is located beneath a sample reaction chamber. The reaction medium supply chamber contains a reaction fluid therein and the reaction chamber contains a thermal reaction medium therein. The reaction fluid is selectively reactive with the thermal reaction medium to produce one of an endothermic or exothermic reaction beneath the sample reaction chamber. | 06-09-2016 |
20160161517 | WORKFLOW TIMING BETWEEN MODULES - The invention relates to a method of isolating and analyzing an analyte using an analytical apparatus which comprises modules of different types, wherein any one module of one type has a specific, pre-defined timing for carrying out its workflow. | 06-09-2016 |
20160169879 | TEST APPARATUS AND METHODS FOR ST2 CARDIAC BIOMARKER | 06-16-2016 |
20160169882 | TEST APPARATUS AND METHODS FOR ST2 CARDIAC BIOMARKER | 06-16-2016 |
20160175836 | System and Method for Processing Fluid in a Fluidic Cartridge | 06-23-2016 |
20160175838 | DEVICES, SYSTEMS, METHODS, AND KITS FOR RECEIVING A SWAB | 06-23-2016 |
20160177384 | SEQUENCING SYSTEMS WITH TWO SLOW-STEP POLYMERASE ENZYMES | 06-23-2016 |
20160178521 | INSTRUMENT FOR MONITORING POLYMERASE CHAIN REACTION OF DNA | 06-23-2016 |
20160178522 | INSTRUMENT FOR MONITORING POLYMERASE CHAIN REACTION OF DNA | 06-23-2016 |
20160184818 | MICRO-FLUIDIC DEVICE - Embodiments described herein provide micro-fluidic systems and devices for use in performing various diagnostic and analytical tests. According to one embodiment, the micro-fluidic device includes a sample chamber for receiving a sample, and a reaction chamber for performing a chemical reaction. A bubble jet pump is structured on the device to control delivery of a fluid from the sample chamber to the reaction chamber. The pump is fluidically coupled to one or more chambers of the device using a fluidic channel such as a capillary. A valve may be coupled to one or more chambers to control flow into and out of those chambers. Also, a sensor may be positioned in one or more of the chambers, such as the reactant chamber, for sensing a property of the fluid within the chamber as well as the presence of a chemical within the chamber. | 06-30-2016 |
20160186240 | DEVICES AND METHODS FOR MOLECULAR DIAGNOSTIC TESTING - A hand-held molecular diagnostic test device includes a housing, an amplification (or PCR) module, and a detection module. The amplification module is configured to receive an input sample, and defines a reaction volume. The amplification module includes a heater such that the amplification module can perform a polymerase chain reaction (PCR) on the input sample. The detection module is configured to receive an output from the amplification module and a reagent formulated to produce a signal that indicates a presence of a target amplicon within the input sample. The amplification module and the detection module are integrated within the housing. | 06-30-2016 |
20160187360 | REAGENT PREPARATION SUPPORT EQUIPMENT, PROGRAM FOR IMPLEMENTATION THEREOF, AND COMPUTER-READABLE RECORDING MEDIUM ON WHICH THIS PROGRAM IS RECORDED - Disclosed herein is a reagent preparation supporting device including: reagent kit information storing means that stores amounts of reagents required for one analysis and a composition of a reaction liquid to be prepared; an input unit that inputs the number of samples to be analyzed; reagent amount calculating means that calls up, from the reagent kit information storing means, the data regarding the reagent kit to calculate required amounts of the reagents included in the reagent kit and a composition of the reaction liquid according to the number of samples inputted through the input unit; and a display unit that displays, as a reagent preparation table, the required amounts of the reagents included in the reagent kit and the composition of the reaction liquid to be prepared based on results calculated by the reagent amount calculating means. | 06-30-2016 |
20160193603 | SAMPLE-TO-ANSWER MICROFLUIDIC CARTRIDGE | 07-07-2016 |
20160199835 | MICROFLUIDIC DEVICE | 07-14-2016 |
20160199838 | SYSTEM AND METHOD FOR CAPTURING AND ANALYZING CELLS | 07-14-2016 |
20160202184 | Devices for Real-Time Polymerase Chain Reaction | 07-14-2016 |
20160202254 | NANOSTRUCTURED DEVICES FOR DETECTING AND ANALYZING BIOMOLECULES | 07-14-2016 |
20160377593 | SINGLE-USE HANDHELD DIAGNOSTIC TEST DEVICE, AND AN ASSOCIATED SYSTEM AND METHOD FOR TESTING BIOLOGICAL AND ENVIRONMENTAL TEST SAMPLES - In a single-use handheld diagnostic test device and an associated system and method, a biological and/or environmental test sample is received and reacted with reagents. The test device tests a single sample and includes a sensor to detect test data. The test device mates with, and transmits the test data to, an electronic device. A processor of the electronic device applies algorithms to the test data to generate highly sensitive and accurate quantitative test results. A presentation element of the electronic device presents the test results to a user. The test device is adapted for disposal, or for sterilization and re-use, after the electronic device receives the test data. The electronic device may be, for example, a mobile communications device, a personal digital assistant, a laptop computer, a navigation device, a digital audio player, a camera, or a gaming device. | 12-29-2016 |
20160377614 | Process and Materials for the Rapid Detection of Streptococcus Pneumoniae Employing Purified Antigen-Specific Antibodies - Disclosed is a cell wall C-polysaccharide antigen of | 12-29-2016 |
20160378953 | GENETIC CHROMOSOME TEST MANAGEMENT SYSTEM, TEST MANAGEMENT SERVER, CLIENT TERMINAL, GENETIC CHROMOSOME TEST MANAGEMENT METHOD, AND PROGRAM - The genetic chromosome test management system ( | 12-29-2016 |
20160378954 | GENETIC CHROMOSOME TEST MANAGEMENT SYSTEM, TEST MANAGEMENT SERVER, CLIENT TERMINAL, GENETIC CHROMOSOME TEST MANAGEMENT METHOD, AND PROGRAM - A genetic chromosome test management system used for a genetic chromosome test for testing an abnormality of a chromosome contained in fetus-derived nucleated red blood cells includes: a test progress detection unit which detects a progress condition of a test until at least a specific step one step before a step which is determined as not being affected by removal of nuclei is completed, with respect to a plurality of specimens; a test progress management unit which manages progress information based on the detected progress condition; and a reception unit, wherein, in a case where the test request is received from the reception unit, the test progress management unit transmits at least one of information pieces of whether the test request is acceptable, the progress condition of the test, or completion expectation of the test, to the reception unit based on the progress information. | 12-29-2016 |
20170232441 | APPARATUS AND METHOD FOR THERMOCYCLIC BIOCHEMICAL OPERATIONS | 08-17-2017 |
20170234803 | IMAGE CAPTURE DEVICE AND ELECTRONIC APPARATUS | 08-17-2017 |
20180021783 | INSTRUMENT FOR PERFORMING A DIAGNOSTIC TEST ON A FLUIDIC CARTRIDGE | 01-25-2018 |
20180023121 | SYSTEM AND CARTRIDGE FOR EFFICIENT NUCLEIC ACID TESTING | 01-25-2018 |
20180024057 | MEASUREMENT DEVICE | 01-25-2018 |
20180024128 | IMMUNOASSAY APPARATUS | 01-25-2018 |
20190145864 | IMMUNOCHROMATOGRAPHIC TEST PIECE AND SPECIMEN ADDING DEVICE FOR EXTRACTING AND MEASURING SUGAR CHAIN ANTIGEN, AND IMMUNOCHROMATOGRAPHY METHOD USING SAME | 05-16-2019 |
20220137044 | METHOD FOR FUNCTIONALIZING A SURFACE, PRODUCT HAVING A SURFACE FUNCTIONALIZED ACCORDING TO SAID METHOD, AND USES THEREOF - A method functionalizes a surface, wherein a polyphenol layer is formed on the surface by applying a polyphenol compound, and the surface is subsequently functionalized by binding a functional molecule to the polyphenol layer. In addition, the polyphenol layer is treated with an oxidizing agent before, during, or after binding of the functional molecule. An associated article can be prepared and used. A kit can be used for performing the method or producing the article. | 05-05-2022 |