Class / Patent application number | Description | Number of patent applications / Date published |
435381000 | Digesting tissue with protease | 23 |
20100068811 | Cardiac Stem Cells - Human cardiac stem cells can be isolated from endomyocardial biopsies. Such cells mediate cardiac regeneration and improve heart function in a mouse infarct model. The cells can be used for autologous, allogeneic, syngeneic, or xenogeneic therapeutic applications in patients. The stem cells can be genetically modified to enhance their therapeutic activity. | 03-18-2010 |
20100112695 | Apparatus And Methods For Processing Tissue to Release Cells - An apparatus and methods for processing tissue to release biological material including cells are disclosed. | 05-06-2010 |
20100112696 | Apparatus And Methods For Processing Tissue To Release Cells - An apparatus and methods for processing tissue to release biological material including cells are disclosed. | 05-06-2010 |
20100112697 | PROCESS FOR THE HIGH-PURITY ISOLATION OF MESENCHYMAL STEM CELLS DERIVED FROM PLACENTAL CHORIONIC PLATE MEMBRANE - The present invention provides a method for isolating mesenchymal stem cells derived from a placental chorionic plate membrane, the method including: (a) harvesting a chorionic plate membrane from a detached placenta; (b) harvesting cells present in the chorionic plate membrane obtained in step (a) by scraping; (c) adding a solution containing trypsin and ethylenediaminetetraacetate to the cells obtained in step (b) to perform an enzymatic reaction and adding a fetal bovine serum thereto to terminate the enzymatic reaction; and (d) centrifuging the reaction solution obtained in step (c) and culturing the obtained cells in a medium containing a fetal bovine serum and an antibiotic. | 05-06-2010 |
20100144036 | ISOLATION OF CELLS - The invention relates to a method for isolating cells from a tissue sample. In preferred embodiments, chondrocytes are isolated from cartilage tissue in a shorter time than hitherto considered possible. | 06-10-2010 |
20100190252 | METHODS FOR THE PREPARATION OF FIBROBLASTS - The invention relates to a process for generating fibroblasts, more particularly, to the culturing of fibroblasts in large numbers and of the heterogenic type. The invention is also directed to the use of fibroblasts in the preparation of heterotypic spheroids and a process for the preparation of such heterotypic spheroids. | 07-29-2010 |
20110076769 | MAMMARY ARTERY DERIVED CELLS AND METHODS OF USE IN TISSUE REPAIR AND REGENERATION - An isolated mammalian internal mammary artery-derived cell is disclosed. Furthermore, methods of isolating the mammalian internal mammary artery-derived cell are disclosed. The cell is useful in tissue engineering technologies, specifically in vascular tissue engineering. | 03-31-2011 |
20110263019 | Method of Reseeding Adherent Cells Grown in a Hollow Fiber Bioreactor System - This invention is directed to methods of directly reseeding harvested adherent cells grown in a hollow fiber bioreactor. Also disclosed is a novel harvest media for use in directly reseeding adherent cells into a hollow fiber bioreactor. | 10-27-2011 |
20120164732 | CANCER TISSUE-DERIVED CELL MASS AND A PROCESS FOR PREPARING SAME - Disclosed is a novel cell mass derived from a cancer tissue, which can reflect the in vivo behavior of a cancer cell correctly. Also disclosed is a process for preparing the cell mass. Specifically disclosed is a cell mass derived from a cancer tissue, which is an separated product that is isolated from a cancer tissue obtained from an individual as a mass containing at least three cancer cells or a cultured product of the separated product and which can retain a proliferation ability in vitro. The cell mass derived from a cancer tissue is produced by, for example, a preparation process comprising the steps of: treating a pulverized product of a cancer tissue removed from a living body with an enzyme; and selecting and collecting a mass containing at least three cancer cells among from an enzymatic treatment product. | 06-28-2012 |
20120252121 | Culture Method for Amplifying Large Numbers of Hair Follicle Stem Cells in Vitro - The present invention is a method, belonging to the field of cell culture, for amplifying a large numbers of hair follicle stem cells in vitro by using microspheres as carriers for cell culture and a revolving bottle as a fermentation tank for cell proliferation. The method is simple in its procedures, is economical and practical, and avoids side effects associated with the traditional cell amplification method, such as the weakening of cell proliferation capability, the reduction of differentiation potential and the like caused by repeated cell subculture, and also reduces the consumption of the culture solution; the hair follicle stem cells amplified by the method can still keep the original proliferation capability and differentiation potential, and can be used for: (1) establishing hair follicle stem cell bank to provide high-quality seed cells for related research on adult stem cells; (2) transplanting and repairing tissues and organs subjected to pathological changes; (3) constructing tissue-engineered organs to be used as substitute autologous organs for transplantation or repair of organs subjected to pathological changes or missing organs; and (4) serving as target cells for the genetic treatment of corresponding diseases. | 10-04-2012 |
20120276629 | METHODS FOR ISOLATING STEM CELLS - Disclosed is a method for isolating stem cells. The method uses a specially designed apparatus including: a container containing an aspirate; a piston having an outer diameter corresponding to the inner diameter of the container and having at least one through-hole; and a connection tube adapted to feed an enzyme or a washing solution into the container through the through-hole, having a tip connected to the through-hole, and connected to an external tube or another container containing the enzyme or washing solution at the other end thereof. The method includes pulling the piston backward to form a negative pressure in the container containing the aspirate and to allow the enzyme or washing solution to enter the container containing the aspirate through the connection tube and the through-hole of the piston. | 11-01-2012 |
20130065305 | MAMMARY ARTERY DERIVED CELLS AND METHODS OF USE IN TISSUE REPAIR AND REGENERATION - An isolated mammalian internal mammary artery-derived cell is disclosed. Furthermore, methods of isolating the mammalian internal mammary artery-derived cell are disclosed. The cell is useful in tissue engineering technologies, specifically in vascular tissue engineering. | 03-14-2013 |
20130130381 | Method for obtaining a population of stromal progenitor cells - The present invention relates to a technique for obtaining stromal progenitor cells (SPC) from adipose tissue (AT) using incubation of very small volumes of AT with an enzyme solution, that can obtain SPC for autologous SPC-based medical applications to a greater number of individuals, possibly having a lower Body Mass Index (B.M.I), i.e. lower than | 05-23-2013 |
20130143324 | PROCESS FOR THE PREPARATION OF DISINFECTED HUMAN CELL SUSPENSIONS - The invention relates to a process for the preparation of disinfected single-cell preparations from mammalian tissues and to the preparations thus prepared. | 06-06-2013 |
20130177986 | PANCREATIC ISLET SEPARATION METHOD, AND PROTECTIVE SOLUTION FOR PROTECTING PANCREATIC ISLET TISSUE - The disclosed islet isolation method comprises: an injection step of injecting a preservation solution into the pancreatic duct of an excised pancreas; a preservation step of immersing the pancreas into an immersion fluid for preservation; a digestion step of breaking down the pancreas to provide pancreatic tissue; and a purification step of immersing the pancreatic tissue in a purification solution to provide islets. The digestion step consists of: an enzyme injection step of injecting an enzyme solution containing a digestion enzyme into the pancreas; a digestion initiation step of activating the digestion enzyme; a digestion termination step of inactivating the digestion enzyme; and a collection step of collecting the broken-down pancreatic tissue. The islet isolation method is characterized in that, by adding a neutrophil elastase inhibitor to the system before the digestion initiation step, the neutrophil elastase inhibitor is present inside the pancreas at the time point of starting the digestion initiation step. By using the above method and a protective solution which can be used in the method, islets having a size and shape suitable for transplantation can be obtained in high yields. | 07-11-2013 |
20130183756 | MAMMARY ARTERY DERIVED CELLS AND METHODS OF USE IN TISSUE REPAIR AND REGENERATION - An isolated mammalian internal mammary artery-derived cell is disclosed. | 07-18-2013 |
20130316455 | METHOD OF ISOLATING CELLS FROM A TISSUE IN A MAMMAL - Solutions and suspensions comprising polymerized hemoglobin derived from human blood are disclosed. The solutions and suspensions may comprise cell culture medium, an enzyme (such as a protease), and/or a buffer. Processes of preparing the solutions and suspensions are also disclosed. The solutions and suspensions may be employed in methods of isolating mammalian cells, such as pancreatic islets, methods of preserving mammalian tissue and organs, methods of aiding the recovery of mammalian cells following their isolation, methods of maintaining mammalian cells, methods of propagating mammalian cells, and methods of treating a mammal with diabetes. | 11-28-2013 |
20140024115 | METHODS OF COMBINING MESENCHYMAL STEM CELLS AND CARTILAGE CONTAINING ALLOGRAFTS, AND PRODUCTS OF COMBINED MESENCHYMAL STEM CELLS AND CARTILAGE CONTAINING ALLOGRAFTS - There is disclosed a method of combining mesenchymal stem cells (MSCs) with an osteochondral allograft. In an embodiment, the method includes obtaining adipose tissue having MSCs with unwanted cells. The tissue is digested to form a cell suspension having MSCs and unwanted cells. The suspension is added to seed the allograft. The MSCs are allowed to attach to the allograft. There is disclosed an allograft product including MSCs with an osteochondral allograft. There is disclosed a method of combining MSCs with decellularized, morselized cartilage. In an embodiment, the method includes obtaining adipose tissue having MSCs with unwanted cells. The tissue is digested to form a cell suspension having MSCs and unwanted cells. The suspension is added to seed the cartilage. The MSCs are allowed to attach to the cartilage to. There is disclosed an allograft product including MSCs with morselized cartilage. Other embodiments are also disclosed. | 01-23-2014 |
20140093961 | METHOD OF WASHING ADHERENT CELL USING TREHALOSE-CONTAINING CELL-WASHING SOLUTION - Methods of washing adherent cells, capable of effectively suppressing cell death due to proteolytic enzyme treatment for detaching the adherent cell from a culture vessel and subsequent cell treatment; cell-washing solutions used for the washing method; methods of producing cell suspensions for transplantation using the cell-washing solution; and kits comprising the cell-washing solution. Trehalose or its derivative or a salt thereof is added to physiological aqueous solutions to prepare cell-washing solutions containing trehalose or its derivative or a salt thereof as an active ingredient. The cell-washing solutions can be used to wash adherent cells before detaching the adherent cells from a culture vessel by proteolytic enzyme treatment to suppress cell death due to the proteolytic enzyme treatment. The concentration of trehalose applied to the cell-washing solution may be a concentration capable of suppressing the cell death due to the proteolytic enzyme treatment, such as 0.1 to 20 (w/v)%. | 04-03-2014 |
20140302605 | Method of obtaining a population of cells - A method of obtaining stromal progenitor cells (SPC) from subcutaneous adipose tissue by incubation of a very small volume of the subcutaneous adipose tissue in an enzyme solution produces SPC that are usable in medical applications based on autologous SPC even on individuals having a body mass index lower than 18.5. | 10-09-2014 |
20150072428 | PROCESS FOR THE PREPARATION OF DISINFECTED HUMAN CELL SUSPENSIONS - In a process for preparing disinfected preparations from mammalian tissues, mammalian tissue is perfused at one step with an enzyme-free liquid antibiotic composition that includes at least one antibiotic and is contained in at least one perfusion buffer. In a step occurring after such one step, enzymatic antibiotic-free treatment of the tissue is carried out to obtain a single-cell suspension. In another step disinfected preparations are obtained. | 03-12-2015 |
20150359941 | METHOD FOR PROCESSING CANCELLOUS BONE MATERIAL AND RELATED PRODUCTS, METHODS AND USES - Cancellous bone material may be processed in a portable container apparatus to prepare a stromal vascular fraction concentrate. Cancellous bone material may be washed to remove non-bone material, digested and digested material centrifuged, with all operations being performed on cancellous bone material while disposed in the portable container apparatus. Uncultured stromal vascular fraction cells separated from enzyme-digested cancellous bone material, and which contain osteoblasts and osteoclasts, may be removed from the portable container and used without culturing for a variety of medical applications. Medical treatment compositions may be prepared including recovered stromal vascular fraction cells and scaffold material. | 12-17-2015 |
20150368615 | METHOD FOR SEPARATING CELL FROM BIOLOGICAL TISSUE - Provided is a method for efficiently and stably separating cells having a high biological activity from a biological tissue, by using a degrading-enzyme composition, which is prepared by adding an enzyme for degrading a major protein of the biological tissue in an amount determined depending on the composition of the major protein to a predetermined amount of a neutral protease and/or a protease derived from | 12-24-2015 |