Entries |
Document | Title | Date |
20080274535 | Hepatocyte Bioreactor System For Long Term Culture of Functional Hepatocyte Spheroids - A rotating wall vessel is used as a culture vessel and bioreactor for the cultivation of hepatocytes in the form of spheroids to generate a culture with many properties of the intact liver. These properties include enzyme activity comparable to fresh cells and long-term maintenance of viability and cellular function for periods on the order of months. The cultures may be used to produce hepatocyte products, evaluate-metabolism of an agent, propagate Hepatitis C virus and test agents as inhibitors of this virus. Thus, the culture system disclosed herein makes long term functional cultivation of human hepatocytes feasible. | 11-06-2008 |
20080286850 | MDCK CELL LINES SUPPORTING VIRAL GROWTH TO HIGH TITERS AND BIOREACTOR PROCESS USING THE SAME - The present invention relates to novel MDCK cells which can be to grow viruses, e.g., influenza viruses, in cell culture to higher titer than previously possible. The MDCK cells can be adapted to serum-free culture medium. The present invention further relates to cell culture compositions comprising the MDCK cells and cultivation methods for growing the MDCK cells. The present invention further relates to methods for producing influenza viruses in cell culture using the MDCK cells of the invention. | 11-20-2008 |
20080293123 | COMPOSITIONS COMPRISING VIRUSES AND METHODS FOR CONCENTRATING VIRUS PREPARATIONS - A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed. | 11-27-2008 |
20080318301 | Methods for Preparing Cells and Viruses - Methods for preparing cells and viruses such as poxviruses are provided herein. | 12-25-2008 |
20090017523 | Virus purification methods - Provided is a method for purifying a virus from a host cell, the method comprising: a) culturing host cells, b) infecting the host cells with a virus, c) treating the cell culture with nuclease, and d) lysing the host cells to provide a lysate comprising the virus. The virus may be recombinant adenovirus. Further provided are methods for purifying a recombinant virus expressing a heterologous protein capable of binding nucleic acid, comprising: a) culturing host cells, b) infecting the host cells with recombinant virus, c) lysing the host cells to provide a lysate comprising the recombinant virus, d) subjecting the recombinant virus to anion exchange chromatography and size exclusion chromatography, wherein the virus-containing mixture is buffer exchanged at least once with a solution comprising at least 2 M NaCl, or another salt providing an equivalent ionic strength. | 01-15-2009 |
20090023197 | Method for purifying a viral suspension - The invention relates to a method for obtaining viruses from a liquid viral suspension, according to which:
| 01-22-2009 |
20090035837 | Virus Production - An improved process for recovery of virus from allantoic fluid of virus-infected chick embryos. Virus associated with granular and fibrous debris in the allantoic fluid can be disassociated from the debris and recovered, thereby increasing viral yield. Dissociation can be achieved by subjecting the virus-debris complex to conditions of increased salt concentrations, e.g., 0.5 M or greater. | 02-05-2009 |
20090035838 | Microfabricated Crossflow Devices and Methods - A microfluidic device for analyzing and/or sorting biological materials (e.g., molecules such as polynucleotides and polypeptides, including proteins and enzymes; viruses and cells) and methods for its use are provided. The device and methods of the invention are useful for sorting particles, e.g. virions. The invention is also useful for high throughput screening, e.g. combinatorial screening. The microfluidic device comprises a main channel and an inlet region in communication with the main channel at a droplet extrusion region. Droplets of solution containing the biological material are deposited into the main channel through the droplet extrusion region. A fluid different from and incompatible with the solution containing the biological material flows through the main channel so that the droplets containing the biological material do not diffuse or mix. Biological material within the droplets can be analyzed and/or sorted by detecting a predetermined characteristic of the biological sample in each droplet and sorting the droplet accordingly. | 02-05-2009 |
20090042274 | Method of Purifying Virus Envelope - An industrial purification method of a virus (e.g., Hemagglutinating Virus of Japan, HVJ) envelope is provided. To be specific, a method of purifying an inactivated virus envelope at a high recovery rate by ion exchange chromatography and hydrophobic chromatography, while maintaining the cell fusion activity of the virus, is provided. The purified virus envelope can be used as a vector for introducing a biopolymer such as gene and the like into a cell or a living organism. In addition, this method can be used for purification of an attenuated envelope virus. | 02-12-2009 |
20090123988 | METHOD FOR THE SPECIFIC OR NON-SPECIFIC SEPARATION OF CELLS AND/OR VIRUSES FROM LIQUID MEDIA AND THE USE THEREOF - The invention relates to a method for the specific or non-specific separation of cells and/or viruses from liquid media which is based on adsorption of the species on a correspondingly functionalised carrier material. The method according to the invention is used for separation and also isolation of any species of cells and viruses. | 05-14-2009 |
20090123989 | VIRUS PURIFICATION USING ULTRAFILTRATION - The invention provides a method for the purification, of a virus comprising a step of ultrafiltration wherein the reteniate contains the virus, wherein back pressure of at least 5 kPa is applied on the permeate side. The invention also provides a method for purification of a recombinant adenovirus, said method consisting essentially of: a) culturing cells that are infected with said recombinant adenovirus, b) lysing said cells and removing free nucleic acid, to provide a lysate comprising the recombinant adenovirus, c) clarifying the lysate to obtain an adenovirus preparation, d) subject the adenovirus preparation to ultrafiltration, wherein the adenovirus preparation is in the retentate, to concentrate the adenovirus preparation, e) subjecting the adenovirus preparation of step d) to ultrafiltration, wherein the adenovirus preparation is in the retenate and exchanging it with at least 5 diafiltration volumes (DFVs) of buffer, wherein in steps d) and e) back pressure of at least 5 kPa is applied on the permeate side. | 05-14-2009 |
20090130738 | Media for membrane ion exchange chromatography - Media for chromatographic applications, wherein the media is a membrane having a surface coated with a polymer such as a polyethyleneimine. The immobilized polymer coating is modified with a charge-modifying agent to impart quaternary ammonium functionality to the media. The media is well suited for chromatographic purification of virus. | 05-21-2009 |
20090275107 | Scalable Production Method for AAV - A method for producing AAV, without requiring cell lysis, is described. The method involves harvesting AAV from the supernatant. For AAV having capsids with a heparin binding site, the method involves modifying the AAV capsids and/or the culture conditions to ablate the binding between the AAV heparin binding site and the cells, thereby allowing the AAV to pass into the supernatant, i.e., media. Thus, the method of the invention provides supernatant containing high yields of AAV which have a higher degree of purity from cell membranes and intracellular materials, as compared to AAV produced using methods using a cell lysis step. | 11-05-2009 |
20090311770 | Method of collecting microorganisms using fine particles, method of collecting nucleic acids using fine particles, and kits for use in the these methods - The present invention provides a method of collecting microorganisms and a method of collecting nucleic acids, which both can be carried out easily and can achieve a high collection rate. A method of collecting microorganisms according to the present invention includes a microorganism adsorption step of bringing a sample into contact with fine particles so as to cause microorganisms contained in the sample to be adsorbed onto the fine particles. In this method, the fine particles have a particle diameter of 6 μm or less and a specific surface area of 50 m | 12-17-2009 |
20100055763 | METHOD FOR THE PRODUCTION AND PURIFICATION OF RETROVIRAL VECTORS - The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production with cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge. | 03-04-2010 |
20100093059 | Method for the Preparation of Sulfated Cellulose Membranes and Sulfated Cellulose Membranes - The present invention relates to methods for the preparation of functionalized sulfated cellulose membranes. In particular, the present invention relates to the preparation of sulfated cellulose membranes under specific reaction conditions allowing to provide a sulfated cellulose membrane useful for pseudo-affinity purification. In another aspect, the present invention relates to the sulfated cellulose membrane itself as well as its use for isolation of proteinaceous compositions. Finally, the present invention provides a method for isolating whole virus, virus proteins or heparin binding molecues comprising the step of affinity purification using the sulfated cellulose membrane according to the present invention. | 04-15-2010 |
20100099163 | SEPARATION MATRIX FOR VIRAL PURIFICATION - The present invention relates to a method of preparing a separation matrix comprising at least one insoluble carrier to which sulphate ligands have been attached via extenders, which method comprises coupling, in a first step, of the sulphate ligands to the extenders and, in a subsequent step, attaching the extenders to an insoluble carrier. The invention also relates to a separation matrix comprised of at least one insoluble carrier to which sulphate ligands have been attached via extenders. Advantageously, no sulphate ligands are directly attached to the insoluble carrier. The extenders may be natural polymers, such as dextran, and the insoluble carrier may be made from natural polymers, such as agarose, or synthetic polymers. The invention also relates to a method of purifying virus, such as influenza virus, using a separation matrix according to the invention. | 04-22-2010 |
20100105124 | NOVEL METHOD FOR THE PRODUCTION AND PURIFICATION OF ADENOVIRAL VECTORS - The present invention relates to improved methods for producing adenovirus compositions wherein host cells are grown in a bioreactor and purified by size partitioning purification to provide purified adenovirus compositions. | 04-29-2010 |
20100112669 | MDCK CELLS LINES SUPPORTING VIRAL GROWTH TO HIGH TITERS AND BIOREACTOR PROCESS USING THE SAME - The present invention relates to novel MDCK cells which can be to grow viruses, e.g., influenza viruses, in cell culture to higher titer than previously possible. The MDCK cells can be adapted to serum-free culture medium. The present invention further relates to cell culture compositions comprising the MDCK cells and cultivation methods for growing the MDCK cells. The present invention further relates to methods for producing influenza viruses in cell culture using the MDCK cells of the invention. | 05-06-2010 |
20100144016 | APPARATUS FOR THE DISRUPTION OF ANIMAL CELLS - The present invention relates to the field of purifying recombinant virus propagated in animal cells. More particularly, it relates to a method for extracting virus from virus-infected cells that have been grown in culture in order to release virus and to apparatus for extracting virus from virus-infected cultured cells using the methods as described herein. | 06-10-2010 |
20100227376 | METHOD OF OBTAINING BACTERIOPHAGE PREPARATION - New method of removing pyrogens from bacteriophage preparations based on the natural processes of endotoxin degradation, and consisting of several stages, including in particular the addition of metal ions, the heterophase extraction, and chromatography. | 09-09-2010 |
20100261254 | BACULOVIRAL VECTORS COMPRISING REPEATED CODING SEQUENCES WITH DIFFERENTIAL CODON BIASES - The present invention relates to production of proteins in insect cells whereby repeated coding sequences are used in baculoviral vectors. In particular the invention relates to the production of parvoviral vectors that may be used in gene therapy and to improvements in expression of the viral rep proteins that increase the productivity of parvoviral vectors. | 10-14-2010 |
20100273238 | Cellular Permissivity Factor for Viruses and Uses Thereof - The present invention provides methods and compositions related to the generation of host cells permissive for virus growth, particularly Porcine Reproductive and Respiratory Syndrome (PRRS) virus. | 10-28-2010 |
20100279385 | PURIFICATION OF ADENOVIRUS AND AAV - The present invention relates to the purification of large scale quantities of active (infectious) adenovirus and AAV, especially for use in therapeutic applications. In particular, the invention provides improved methods for contacting such viruses with suitable chromatographic materials in a fashion such that any damage to the virus, particularly to surface components thereof, resulting from contact with such chromatographic materials is minimized or eliminated. The result is the ability to rapidly and efficiently purify commercial level quantities of active (infectious) virus suitable for use in therapeutic applications, e.g. gene transfer/therapy procedures. | 11-04-2010 |
20100279386 | MODIFIED VACCINIA ANKARA VIRUS VARIANT AND CULTIVATION METHOD - The present invention provides an attenuated virus, which is derived from Modified Vaccinia Ankara virus and characterized by the loss of its capability to reproductively replicate in human cell lines. It further describes recombinant viruses derived from this virus and the use of the virus, or its recombinants, as a medicament or vaccine. A method is provided for inducing an immune response in individuals who may be immune-compromised, receiving antiviral therapy, or have a pre-existing immunity to the vaccine virus. In addition, a method is provided for the administration of a therapeutically effective amount of the virus, or its recombinants, in a vaccinia virus prime/vaccinia virus boost innoculation regimen. The present invention relates to a method of virus amplification in primary cells which are cultivated in a serum free medium. Viruses produced by this method are advantageously free of any infectious agents comprised in animal sera. | 11-04-2010 |
20100297733 | Systems And Methods For The Capture And Separation Of Microparticles - Systems and methods are provided for capturing and/or isolating target microparticles. In one aspect, a method for capturing target microparticles is disclosed. The method includes: forming a fluid including the target microparticles, non-target microparticles, and magnetic beads, the magnetic beads having a stronger affinity with the target microparticles than with the non-target microparticles; flowing the fluid through a multidirectional microchannel; and applying a magnetic field to the fluid while the fluid is flowing through at least a portion of the microchannel to effect capture of at least a portion of the target microparticles onto the magnetic beads. Such a method can further includes passing the fluid having exited from the microchannel through a separator while subjecting the fluid to a second magnetic field so as to isolate the target microparticles. In addition, devices and systems are disclosed for capturing and/or isolating target microparticles based on magnetic manipulation. | 11-25-2010 |
20100323429 | Methods for purifying baculovirus - The present invention provides a method for purifying baculovirus comprising: providing a baculovirus mixture containing a baculovirus and a liquid portion; replacing the liquid portion with a binding buffer by an ultrafiltration system to form a virus buffer; and purifying the baculovirus from the virus buffer using glycoprotein specific affinity chromatography. Therefore, use of the method of the present invention in the purification of baculovirus resulted in an enhanced discovery yield and improved purity of virus. | 12-23-2010 |
20100323430 | Media For Membrane Ion Exchange Chromatography - Media for chromatographic applications, wherein the media is a membrane having a surface coated with a polymer such as a polyethyleneimine. The immobilized polymer coating is modified with a charge-modifying agent to impart quaternary ammonium functionality to the media. The media is well suited for chromatographic purification of virus. | 12-23-2010 |
20100330650 | RECOMBINANT VACCINE AGAINST JAPANESE ENCEPHALITIS VIRUS [JEV] INFECTION AND A METHOD THEREOF - The present invention relates to a method for preparing recombinant adenovirus (RadEs) vaccine to protect against JEV infection. The vaccine produces secretory envelop protein (ES) of JEV. | 12-30-2010 |
20110008873 | PURIFIED BACTERIOPHAGE, ITS PREPARATION AND APPLICATION - A method of preparation of purified bacteriophage with increased antibacterial activity, in which from bacterial lysate of phages is obtained, advantageously in the presence of lysozyme, chelating factor and detergent, in a continuous manner with ultrafiltration on membranes, the phage containing high molecular mass preparation, devoid of bacterial cell wall and other contaminants, free of toxins and endotoxins, active in tests of bacterial lysis, which is characterized by chromatography HPLC, In SDS-PAGE electrophoresis, immunoblotting, biological tests of bacterial lysis, and is dedicated for phage therapy of bacterial infections and tumors and for production of phage deriving pharmaceutical preparations. | 01-13-2011 |
20110045574 | CHROMATOGRAPHY MEDIUM - The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there. | 02-24-2011 |
20110053250 | ENRICHMENT METHOD OF VIRUS - The present invention provides a novel method that can increase readily a virus or viral vector concentration in a solution having a low concentration and a kit for performing the method. Conventional methods require complicated operations, expensive equipment, or highly trained experts for efficiently concentrating viruses from low-concentration virus solutions. The method of the present invention can concentrate viral vectors readily while maintaining infection abilities of the viral vectors, and thus it can be used as a safe and simple technique for concentrating a vector useful in the field of a genetic therapy or a vaccine therapy using a viral vector. | 03-03-2011 |
20110076744 | Compositions and methods to prevent AAV vector aggregation - Compositions and methods are provided for preparation of concentrated stock solutions of AAV virions without aggregation. Formulations for AAV preparation and storage are high ionic strength solutions (e.g. μ˜500 mM) that are nonetheless isotonic with the intended target tissue. This combination of high ionic strength and modest osmolarity is achieved using salts of high valency, such as sodium citrate. AAV stock solutions up to 6.4×10 | 03-31-2011 |
20110097785 | METHOD OF REPLICATING VIRUSES IN SUSPENSION CULTURES OF DOG KIDNEY CELLS - Animal cells are described which can be infected by viruses and which are adapted to growth in suspension in medium free of animal-derived components, such as serum-free medium. Processes for the replication of viruses in cell culture using these cells are furthermore described, as well as vaccines which contain the viruses or antigenic portions thereof obtainable by the process. | 04-28-2011 |
20110117628 | METHOD FOR CONCENTRATING AND ISOLATING BIOMOLECULES OR VIRUSES - A simple and convenient method for concentrating a biomolecule, including protein or nucleic acid molecules, from a sample. Purified and isolated biomolecules obtained by this method. Methods for improving the specificity or sensitivity of detecting a biomolecule by concentration and/or purification or isolation of the biomolecule according to the method of the invention. | 05-19-2011 |
20110124086 | Hepatitis C Virus Culture System - This disclosure provides compositions and methods for producing infectious hepatitis C virus (HCV). The produced HCV can be infectious in vivo and in vitro. In one aspect, the disclosure provides an immortalized primary hepatocyte transformed with a DNA construct comprising a cDNA sequence of HCV. | 05-26-2011 |
20110151543 | CELL SEPARATION METHOD USING HYDROPHOBIC SOLID SUPPORTS - Provided is a method of separating cells using a hydrophobic solid support. The method comprises contacting a solution containing cells with a hydrophobic solid support having a water contact angle between 70 and 90 degrees. By allowing cells to be adsorbed to the hydrophobic solid substrate, the cells can be separated. Thus, the cell separation efficiency can be rapidly and simply increased. | 06-23-2011 |
20110207202 | METHOD FOR THE PRODUCTION OF ADENOVIRAL VECTORS - The invention provides methods for large-scale production of recombinant adenovirus 35, using perfusion systems and infection at very high-cell densities. | 08-25-2011 |
20110217757 | MODIFIED VACCINIA ANKARA VIRUS VARIANT AND CULTIVATION METHOD - The present invention provides an attenuated virus, which is derived from Modified Vaccinia Ankara virus and characterized by the loss of its capability to reproductively replicate in human cell lines. It further describes recombinant viruses derived from this virus and the use of the virus, or its recombinants, as a medicament or vaccine. A method is provided for inducing an immune response in individuals who may be immune-compromised, receiving antiviral therapy, or have a pre-existing immunity to the vaccine virus. In addition, a method is provided for the administration of a therapeutically effective amount of the virus, or its recombinants, in a vaccinia virus prime/vaccinia virus boost inoculation regimen. The present invention relates to a method of virus amplification in primary cells which are cultivated in a serum free medium. Viruses produced by this method are advantageously free of any infectious agents comprised in animal sera. | 09-08-2011 |
20110229957 | POLYMER-MEDIATED ELECTROMAGNETIC FIELD-BASED PARTICLE CONCENTRATOR - There remains an unmet need for methods to efficiently concentrate and isolate small particles, such as nano-sized and micron-sized particles. The methods herein provide means to fulfill this need through the combination of two independent particle-concentrating mechanisms, yielding unprecedented control, confinement, and concentration enhancement ability. The method is carried out in a reversible and spatially controllable manner. Useful applications include diagnostics and bioparticle separation, material science and research uses, among other uses. | 09-22-2011 |
20110281329 | PROCESS AND SYSTEM FOR THE INDUSTRIAL SCALE PURIFICATION OF BACTERIOPHAGES INTENDED FOR BACTERIOPHAGE THERAPY - The present invention is directed towards bacteriophage therapy, the treatment of infectious diseases, caused by pathogenic bacteria, using specific bacteriophages. The purity of bacteriophage preparations has been a major obstacle for their therapeutic use in the past. Current purification methods are only suitable for low-scale production, as they are work and cost intensive. Disclosed is a method and machinery to purify crude bacteriophage preparations in large scale and fully automated, resulting in highly pure (toxin free) and highly concentrated bacteriophage preparations, suitable for agricultural and clinical applications. | 11-17-2011 |
20110306113 | Scalable Process for Protein Purification - The invention provides a process for the purification recombinantly expressed, self-assembled VLP from the homogenate of a bacterial host, wherein the process can be scaled up to a commercial production scale in a cost effective manner. The process comprises a first chromatography using an anion exchange matrix, a second chromatography using hydroxyapatite and, optionally, a size exclusion chromatography. VLP preparations obtained by the process of the invention are essentially free of endotoxin contaminations. | 12-15-2011 |
20110306114 | PURIFICATION OF VACCINIA VIRUSES USING HYDROPHOBIC INTERACTION CHROMATOGRAPHY - The present invention relates to methods for purification of Vaccinia viruses (VV) and/or Vaccinia virus (VV) particles, which can lead to highly pure and stable virus preparations of predominantly biologically active viruses. The invention encompasses purifying a virus preparation in a sterilized way with high efficiency and desirable yield in terms of purity, biological activity and stability, aspects advantageous for industrial production. | 12-15-2011 |
20110312060 | PURIFICATION OF VACCINIA VIRUS- AND RECOMBINANT VACCINIA VIRUS-BASED VACCINES - The present invention relates to methods for purification of Vaccinia viruses (VV) and/or Vaccinia virus (VV) particles, which can lead to highly pure and stable virus preparations of predominantly biologically active viruses. The invention encompasses purifying a virus preparation in a sterilized way with high efficiency and desirable yield in terms of purity, biological activity and stability, aspects advantageous for industrial production. | 12-22-2011 |
20110318814 | METHODS, KITS AND SYSTEMS FOR PROCESSING SAMPLES - A method for isolating microorganisms from a sample, the sample including sample matrix and microorganisms, the method including the steps of providing a receptacle, the receptacle configured to allow filtering of the sample and to reversibly contain the sample and a concentration agent; adding the sample to the receptacle, wherein a microorganism-bound composition will be formed in the receptacle, the microorganism-bound composition including concentration agent-bound microorganisms and sample matrix; and filtering the microorganism-bound composition through a filter to collect the concentration agent-bound microorganisms on the filter, wherein the filter has an average pore size that is greater than the average size of the microorganisms. Kits and systems are also disclosed herein. | 12-29-2011 |
20120015425 | Cellular Permissivity Factor for Viruses and Uses Thereof - The present invention provides methods and compositions related to the generation of host cells permissive for virus growth, particularly Porcine Reproductive and Respiratory Syndrome (PRRS) virus. | 01-19-2012 |
20120064604 | METHODS FOR ENHANCING INFECTIVITY OF RETROVIRUSES - The present invention provides methods for producing retroviruses or viral vectors with enhanced infectivity. The methods entail transfecting a retroviral vector into a packaging cell that has suppressed expression or inhibited enzymatic activity of a parvulin prolyl peptidyl isomerase (parvulin PPIase), and culturing the transfected packaging cell to allow production of viral particles. The invention also provides methods for enhancing efficiency of gene transfer with a recombinant retrovirus. These methods involve constructing a recombinant retroviral vector expressing a target gene, transfecting into a packaging cell that has suppressed expression or inhibited enzymatic activity of a parvulin prolyl peptidyl isomerase (parvulin PPIase), culturing the transfected packaging cell to allow production of recombinant retroviral particles, harvesting recombinant retroviral particles from supernatant of the cultured cell, and transducing the recombinant retroviral particles into a target cell. Kits for carrying out these methods are also provided in the invention. | 03-15-2012 |
20120077249 | Separation Of Virus And/Or Protein From Nucleic Acids By Primary Amines - A method of purifying biomolecules with an anion exchanger containing a membrane having a surface having a polymer such as a primary or secondary amine ligand formed thereon, such as polyallylamine. The feedstock is introduced to the exchanger in the presence of one or more ionic-modifiers by themselves or in combination with monovalent salt. The ionic modifier alters the binding ability of the primary amines such that they retain a significant binding capacity for highly charged species such as DNA but lose part or almost all of their binding capacity for less charged species such as viruses or proteins at pH above the pI of the virus or protein. | 03-29-2012 |
20120115207 | ENHANCED PRODUCTION OF PAPILLOMAVIRUS-LIKE PARTICLES WITH A MODIFIED BACULOVIRUS EXPRESSION SYSTEM - The present invention is concerned with the provision of a method for manufacturing papillomavirus like particles (PV-VLP), comprising the steps of a) culturing a host cell lacking protease activity and comprising an expression vector, wherein said expression vector comprises at least one polynucleotide encoding a PV L1 polypeptide, and b) obtaining VLPs from the host cell. Also proposed is a host cell lacking protease activity and comprising an expression vector, wherein said expression vector comprises a polynucleotide encoding at least one PV L1 polypeptide. Furthermore, a method for the manufacture of a pharmaceutical composition for the treatment or prevention of PV-related disease comprising the steps of manufacturing PV-VLPs and the further step of formulating the VLPs as a pharmaceutical composition is proposed as well as an expression vector comprising at least one polynucleotide encoding a PV L1 polypeptide but lacking a functional gene for a v-cath protease. | 05-10-2012 |
20120142078 | SYSTEM FOR GENERATION OF VIABLE REOVIRUS FROM CLONED CDNA - The present invention provides methods for the generation of viable reoviruses using only cloned nucleic acid segments representing the RNA segments of the reovirus genome. | 06-07-2012 |
20120156757 | PURIFICATION OF IMMUNOGENS USING A NON-POLYSACCHARIDE MATRIX - The present invention relates, at least in part, to novel and improved chromatography matrices for separating or purifying immunogens, such as, for example, viruses and viral surface proteins, from one or more contaminants in a sample, where the matrix comprises a porous non-polysaccharide solid support comprising a negatively charged, multivalent ion exchange group directly attached to the solid support. | 06-21-2012 |
20120156758 | Recombinant Influenza Viruses for Vaccines and Gene Therapy - The invention provides compositions and methods useful to prepare segmented, negative strand RNA viruses, e.g., orthomyxoviruses such as influenza A viruses, entirely from cloned cDNAs and in the absence of helper virus. | 06-21-2012 |
20120164710 | METHOD OF PREPARATION OF A BIOLOGICAL PARTICULATE STRUCTURE - The invention provides methods for the preparation of an isolated virus particle or virus-like particle by treating with an agent such that the particles are preferentially in the aqueous phase. The invention also provides methods of preparing a capsomere that is substantially-free of at least one host cell derived chaperone protein by treatment with an agent to selectively separate the capsomere from at least one chaperone protein. | 06-28-2012 |
20120171750 | PURIFICATION OF VACCINIA VIRUSES USING HYDROPHOBIC INTERACTION CHROMATOGRAPHY - The present invention relates to methods for purification of Vaccinia viruses (VV) and/or Vaccinia virus (VV) particles, which can lead to highly pure and stable virus preparations of predominantly biologically active viruses. The invention encompasses purifying a virus preparation in a sterilized way with high efficiency and desirable yield in terms of purity, biological activity and stability, aspects advantageous for industrial production. | 07-05-2012 |
20120190100 | ENZYMATIC COMPOSITION FOR THE DIGESTION OF CHICKEN EMBRYOS - The present invention relates to an enzymatic composition for the digestion of chicken embryos intended to the preparation of cells which are used for the production of viruses. The present invention also relates to a method for producing a wild type, an attenuated and/or a recombinant virus comprising a step of preparation of cells from chicken embryos using an enzymatic composition of the invention. The present invention relates to a purified wild type, attenuated and/or recombinant virus obtained and to a pharmaceutical composition, preferably a vaccine, comprising said virus for the treatment and/or the prevention a cancer, an infectious disease and/or an autoimmune disorder, and uses thereof. | 07-26-2012 |
20120202267 | METHOD FOR THE PURIFICATION OF ADENOVIRUS PARTICLES - The invention provides methods for large-scale adenovirus purification from high cell density suspensions, using host cell DNA precipitation followed by a clarification step. | 08-09-2012 |
20120202268 | PROCESS FOR ADENOVIRUS PURIFICATION FROM HIGH CELL DENSITY CULTURES - The invention provides methods for large-scale adenovirus purification from high cell density suspensions, using host cell DNA fragmentation and/or precipitation followed by a clarification step with tangential flow filtration. | 08-09-2012 |
20120231526 | Method for the Purification of Alphavirus Replicon Particles - Methods of production and purification for viruses and virus-derived vectors, including those related to alphaviruses, are disclosed. In one aspect, methods of purification that subject alphavirus replicon particle preparations to one or more steps of chromatographic purification, such as using an ion exchange resin, are provided. Also disclosed are methods of characterizing alphavirus replicon particles and utilizing these materials for vaccines and gene-based therapeutics | 09-13-2012 |
20120238001 | PRODUCTION OF VIRAL VACCINES IN SUSPENSION ON AVIAN EMBRYONIC DERIVED STEM CELL LINES - The present invention relates to the development and manufacturing of viral vaccines. In particular, the invention relates to the field of industrial production of viral vectors and vaccines, more in particular to the use of avian embryonic stem cells, preferably the EBx® cell line derived from chicken embryonic stem cells, for the production of viral vectors and viruses. The invention is particularly useful for the industrial production of viral vaccines to prevent viral infection of humans and animals. | 09-20-2012 |
20120244600 | SWINE INFLUENZA HEMAGGLUTININ VARIANTS - Polypeptides, polynucleotides, methods, compositions, and vaccines comprising influenza hemagglutinin and neuraminidase variants are provided. | 09-27-2012 |
20120252098 | Dissociation of Product-complexed Contaminants in Chromatography - The invention provides methods and materials for using apatite chromatography supports to dissociate and remove contaminants that are complexed to biological products. The invention further provides materials and methods for dissociating aggregations of target biological molecules or improperly folded target molecules to improve purification of the target molecule. | 10-04-2012 |
20120282674 | METHOD FOR THE PREPARATION OF AN INFLUENZA VIRUS - The present invention relates to a method for the preparation of a pharmaceutical composition for the prevention or/and treatment of an influenza virus infection. | 11-08-2012 |
20130023034 | MUTATED REP ENCODING SEQUENCES FOR USE IN AAV PRODUCTION - The invention relates to a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein, wherein a nuclear localization signal (NLS) in said Parvoviral Rep protein is mutated as compared with a corresponding wild type sequence. The invention also relates to a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein, wherein the zinc finger domain in said Parvoviral Rep protein is mutated as compared with a corresponding wild type sequence. Further, the invention relates to a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein, wherein an amino acid at position 43, 57, 79, 97, 120, 179, 305, 484, 493 or 571 of the said Parvoviral Rep protein is mutated in comparison to a corresponding wild type sequence, said amino acid position being defined with reference to SEQ ID NO: 2. | 01-24-2013 |
20130084620 | PROCESS FOR PRODUCING POXVIRUSES AND POXVIRUS COMPOSITIONS - The present invention relates to compositions and pharmaceutical compositions comprising poxviruses and more particularly extracellular enveloped viruses. The present invention also relates to a process for producing poxviruses and poxviruses obtained thereof. Moreover, the present invention also relates to the use of said poxvirus and said composition for the preparation of a medicament. | 04-04-2013 |
20130095558 | PROCESS FOR PRODUCING RECOMBINANT HUMAN ENDOSTATIN ADENOVIRUS - This invention discloses a production process for recombinant human endostatin adenovirus in order to optimize the procedure for small batch and mass industrialization. Exemplary process include steps of: (1) fermentation of eukaryotic cells (HEK293 cells) in the condition of 37° C. and 5% CO | 04-18-2013 |
20130102055 | CONTINUOUS FLOCCULATION DEFLOCCULATION PROCESS FOR EFFICIENT HARVESTING OF MICROALGAE FROM AQUEOUS SOLUTIONS - A continuous process for efficiently harvesting microalgae from aqueous systems is described herein. The method and apparatus of the present invention allows continuous harvesting of algae from a variety of source waters including saltwater, brackish water, fresh water, and treated wastewater. High concentration factors are achievable and the system produces a deflocculated product that is readily processed for biofuel or pharmaceutical applications. The process of the present invention does not add contaminants that can limit the downstream usage possibilities for the algae concentrate produced. The effluent water from the process is suitable for conventional discharge or recycling to the growth system. The process of the present invention is inexpensive, scalable, and generates useful effluent water and algae concentrate as products. | 04-25-2013 |
20130115683 | METHOD FOR THE PREPARATION OF A PHARMACEUTICAL COMPOSITION - The present invention relates to a method for the preparation of a pharmaceutical composition for the prevention or/and treatment of an influenza virus infection. | 05-09-2013 |
20130164821 | METHOD FOR SEPARATING VIRUSES FROM A CONTAMINANT-CONTAINING LIQUID - The present invention relates to a method for separating viruses from a contaminant-containing liquid medium using two adsorbents having cationic groups, wherein the viruses are adsorbed to the first adsorbent and subsequently eluted and wherein the contaminants present in the resulting eluate are subsequently adsorbed to the second adsorbent. The yield and purity of the viruses obtained as per the method according to the invention is increased by the addition of multivalent anions during the adsorption of the contaminants to the second adsorbent. | 06-27-2013 |
20130183741 | MDCK-DERIVED CELL LINES ADAPTED TO SERUM-FREE CULTURE AND SUSPENSION CULTURE AND METHOD FOR PREPARING VACCINE VIRUS USING THE CELLS - Disclosed is a Madin-Darby canine kidney (MDCK)-derived cell line. The MDCK-derived cell line is derived from MDCK cells deposited under accession number ATCC CCL-34. The MDCK-derived cell line can be prepared by serum-free culture and suspension culture. Preferably, the MDCK-derived cell line has low or no tumorigenicity. The MDCK-derived cell line is preferably selected from MDCK Sky1023, MDCK Sky10234 and MDCK Sky3851. Further disclosed are a culture method for growing the MDCK-derived cells and a method for producing a vaccine virus using the MDCK-derived cells. | 07-18-2013 |
20130183742 | METHOD FOR HARVESTING EXPRESSION PRODUCTS - The present invention provides a method for recovering an essentially cell-associated expression product from a host cell comprising (a) culturing said host cell under conditions that allow expression of said expression product; (b) collecting said host cell in/on a filter unit; (c) disrupting said host cell in/on the filter unit; and (d) separating said expression product from said disrupted host cell. Said host cell is preferably a vertebrate cell, more preferably an avian cell, which is preferably cultured in suspension. Furthermore, the present invention provides for the use of a filter unit characterized in that said filter unit is (i) suitable to retain a host cell which expresses an expression product; and (ii) suitable for elution of said expression product from the filter unit after cell disruption in/on said filter unit for recovering said expression product from said host cell as well as for a system for recovering an expression product from a host cell comprising said filter unit. The present invention also provides an expression product obtainable by said method, said expression product being preferably a virus, specifically a poxvirus, in particular selected from the group consisting of fowlpoxvirus, vaccinia virus and, more preferably, modified vaccinia virus Ankara, MVA. | 07-18-2013 |
20130189762 | MULTI PLASMID SYSTEM FOR THE PRODUCTION OF INFLUENZA VIRUS - Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. In addition, the present invention includes an improved method of rescue, wherein animal cells (e.g., SF Vero cells) are electroporated with plasmids and vectors of the invention. | 07-25-2013 |
20130295647 | METHOD OF PRODUCING BACTERIOPHAGE PREPARATIONS COMPRISING PURIFICATION USING AFFINITY CHROMATOGRAPHY - The proposed method facilitates the single-stage and at the same time effective purification of phage preparations for therapeutic uses, and facilitates the maintenance of bacteriophage antibacterial activity both in the case of displacement of the bacteriophage from the resin and its proteolytic release. The protein modification of the phage capsid with appropriate binding motifs makes it possible to purify therapeutically bacteriophage strains using affinity chromatography. The proposed method is useful in the display of selected polypeptided on a bacteriophage capsid without the need to genetically modify the bacteriophage, and thus makes it possible to produce phage preparations for various uses using wild-type phages occurring naturally or others not additionally modified for phage-display purposes. | 11-07-2013 |
20140004595 | LIGAND GRAFT FUNCTIONALIZED SUBSTRATES | 01-02-2014 |
20140051148 | Cells and Methodology to Generate Non-Segmented Negative-Strand RNA Viruses - The present invention relates to recombinant cells as well as to methods for the generation of non-segmented negative-sense single-stranded RNA viruses (NNV or mononegavirales) from cloned deoxyribonucleic acid (cDNA), especially from measles virus and in particular from attenuated strains such as those approved for vaccination, in particular from the attenuated Schwarz measles virus and various recombinant Schwarz measles-based viruses expressing heterologous sequences. Such rescued viruses can be used, after amplification, as vaccines for immunization against measles and/or against the heterologous peptides or proteins expressed. | 02-20-2014 |
20140073032 | BATCHES OF RECOMBINANT ADENOVIRUS WITH ALTERED TERMINAL ENDS - Described is a composition comprising a plurality of recombinant adenovirus particles, being a recombinant human adenovirus of serotype 5, 26, 34, 35, 48, 49 or 50, or a recombinant simian adenovirus, characterized in that the genomes of essentially all adenovirus particles in the composition comprise as the 5′ terminal nucleotides the nucleotide sequence: CTATCTAT (nucleotides 1-8 of SEQ ID NO:7). Also described are methods to produce such compositions. | 03-13-2014 |
20140073033 | METHOD FOR ORTHOPOXVIRUS PRODUCTION AND PURIFICATION - The present invention relates to a method for producing and purifying a wild type, an attenuated and/or a recombinant | 03-13-2014 |
20140073034 | Liquid to Liquid Biological Particle Concentrator with Disposable Fluid Path - Highly efficient and rapid filtration-based concentration devices, systems and methods are disclosed with sample fluidic lines and a filter packaged in a disposable tip which concentrate biological particles that are suspended in liquid from a dilute feed suspension. A sample concentrate or retentate suspension is retained while eliminating the separated fluid in a separate flow stream. The concentrate is then dispensed from the disposable tip in a set volume of elution fluid. Suspended biological particles include such materials as proteins/toxins, viruses, DNA, and/or bacteria in the size range of approximately 0.001 micron to 20 microns diameter. Concentration of these particles is advantageous for detection of target particles in a dilute suspension, because concentrating them into a small volume makes them easier to detect. All conduits by which the disposable tip attaches to the instrument are combined into a single connection point on the upper end of the tip. | 03-13-2014 |
20140087445 | METHOD OF MAKING LIGAND FUNCTIONALIZED SUBSTRATES - Ligand functionalized substrates, methods of making ligand functionalized substrates, and methods of using functionalized substrates are disclosed. | 03-27-2014 |
20140141492 | Cellular Permissivity Factor for Viruses and Uses Thereof - The present invention provides methods and compositions related to the generation of host cells permissive for virus growth, particularly Porcine Reproductive and Respiratory Syndrome (PRRS) virus. | 05-22-2014 |
20140154783 | BIOPROCESSING - The present invention relates to molecular biology, molecular genetics, and bioprocessing. The embodiments provide for compositions and methods for producing a biological product, such as an immunogenic agent, in an embryonated egg by introducing into the egg a RNA effector molecule capable of modulating expression of a target gene, wherein the modulation enhances production of the biological product in the egg. These methods provide for RNAi-based approaches to optimize the production of biologics from embryonated eggs, such as the production of viral vaccines including seasonal and pandemic flu vaccines. The invention also relates to molecules, reagents, cells, and kits useful for carrying out the methods, and biological products produced by the methods. | 06-05-2014 |
20140162342 | METHODS AND COMPOSITIONS FOR PRODUCTION OF VACCINA VIRUS - Certain embodiments are directed to viral production processes for the large scale production of vaccinia virus. | 06-12-2014 |
20140178969 | VIRAL PURIFICATION METHODS - The present invention is directed to an improved method of purifying virus, particularly reovirus. Infectious virus can be extracted from a cell culture with a detergent to produce high titers of virus, and the virus can then be purified by simple steps such as filtration and column chromatography. Viruses and compositions comprising the viruses prepared according to the present invention are also provided. | 06-26-2014 |
20140186926 | BACULOVIRAL VECTORS COMPRISING REPEATED CODING SEQUENCES WITH DIFFERENTIAL CODON BIASES - The present invention relates to production of proteins in insect cells whereby repeated coding sequences are used in baculoviral vectors. In particular the invention relates to the production of parvoviral vectors that may be used in gene therapy and to improvements in expression of the viral rep proteins that increase the productivity of parvoviral vectors. | 07-03-2014 |
20140206062 | Recombinant Influenza Viruses for Vaccines and Gene Therapy - The invention provides compositions and methods useful to prepare segmented, negative strand RNA viruses, e.g., orthomyxoviruses such as influenza A viruses, entirely from cloned cDNAs and in the absence of helper virus. | 07-24-2014 |
20140242671 | Cell Line for Production of Adeno-associated Virus - This invention relates to a HEK293 cell line that grows under animal component-free suspension conditions. The cell line is ideal for rapid and scalable production of adeno-associated virus (AAV) and supports production of all serotypes and chimera of AAV. | 08-28-2014 |
20140273159 | Staged Bacteriophage Remediation of Target Bacteria - A process for remediation of target bacteria, particularly sulfur reducing bacteria (SRB), in waters (“target water”) having a multiplicity and diverse host target bacteria by employing serial or staged bacteria culturing and lysing of dominant bacteria. Remediation of sulfur reducing bacteria (SRB) is effected by application of a series of bacteriophage isolated from the staged culturing and bacteriophage lysing of successive aliquots of waters containing a multiplicity of SRB. | 09-18-2014 |
20140342434 | Removal of contaminating viruses from AAV preparations - The present invention relates to a separation of viruses of an essentially spherical shape from viruses with a rod-like shape that are comprised in a sample, wherein the sample comprising the viruses is subjected to filtration. | 11-20-2014 |
20150010983 | COMPOSITIONS, METHODS AND USES FOR INDUCING VIRAL GROWTH - Embodiments herein report methods, compositions and uses for inducing and/or accelerating viral growth. In certain embodiments, methods, compositions and uses generally related to copolymer compositions for inducing viral growth, reducing lag time and/or increasing viral plaque size. In other embodiments, methods, compositions and uses of copolymer compositions can be for inducing flaviviral growth, reducing lag in growth and/or increasing plaque size. | 01-08-2015 |
20150024467 | METHODS FOR PURIFICATION OF RECOMBINANT AAV VECTORS - Provided herein are methods for the purification of recombinant adeno-associated virus (rAAV) vectors that can be used for gene transfer and specifically for gene therapy or vaccination. Recombinant AAV vectors of the invention are substantially free of in-process impurities, including production components such as cellular nucleic acids, cellular proteins, helper virus, and media components. | 01-22-2015 |
20150037873 | METHOD FOR ENDOTOXIN REMOVAL - The present invention relates to a method for endotoxin removal from a sample comprising the following steps: combining the sample comprising one or more target molecule(s) with a chromatography media comprising beads having an inner porous core functionalized with ligands capable of binding endotoxin and an outer porous layer without functional groups and a pore size small enough to exclude the target molecule from the inner core; and collecting the sample from the media, wherein the sample comprises an endotoxin level which is at least 75% less, preferably 90% less, than before the removal and the yield of the target molecule is at least 75%. | 02-05-2015 |
20150037874 | CHIMERIC ADENOVIRUSES FOR USE IN CANCER TREATMENT - The present invention relates to oncolytic adenoviruses having therapeutic applications. Recombinant chimeric adenoviruses, and methods to produce them are provided. The chimeric adenoviruses of the invention comprise nucleic acid sequences derived from adenoviral serotypes classified within the subgroups B through F and demonstrate an enhanced therapeutic index. | 02-05-2015 |
20150064770 | TUNING BACTERIOPHAGE HOST RANGE - Various aspects and embodiments of the invention are directed to high-throughput phage-engineering methods and recombinant bacteriophages with tunable host ranges for controlling phage specificity. | 03-05-2015 |
20150093804 | METHOD FOR PREPARING VIRUS-LIKE PARTICLE AND RECOMBINANT BACULOVIRUS USED THEREIN - A recombinant baculovirus is provided for preparing picornavirus virus-like particles (VLP), wherein Chitinase A (ChiA) and Cathepsin V (v-cath) genes of the recombinant baculovirus are functionally disrupted and the recombinant baculovirus includes a picornavirus capsid protein gene under control of a strong promoter, and includes a protease gene configured for encoding a protease for hydrolyzing the capsid protein under control of a weak promoter. The recombinant baculovirus of the present invention may adopt High Five or Sf-9 cells for manufacturing enterovirus virus-like particles with improved stability and higher yields in comparison with the conventional arts. A method for preparing virus like particles is also herein provided. | 04-02-2015 |
20150125929 | USE OF CHARGED FLUOROCARBON COMPOSITIONS IN METHODS FOR PURIFICATION OF BIOMOLECULES - The present invention relates to novel and improved methods for the purification of biomolecules. In particular, the present invention relates to methods of protein purification which employ a porous solid support modified with a charged fluorocarbon composition. | 05-07-2015 |
20150299668 | METHOD FOR PRODUCING PARVOVIRUS HAVING HIGH INFECTIVITY TITER - A method for stably and easily producing a parvovirus having a high infectivity titer is provided. The problem is solved by a method for producing a parvovirus having an infectivity titer as high as 10 | 10-22-2015 |
20150322411 | Method of Replicating Viruses in Suspension Cultures of Dog Kidney Cells - Animal cells are described which can be infected by viruses and which are adapted to growth in suspension in medium free of animal-derived components, such as serum-free medium. Processes for the replication of viruses in cell culture using these cells are furthermore described, as well as vaccines which contain the viruses or antigenic portions thereof obtainable by the process. | 11-12-2015 |
20150352465 | Chromatography Media For Purifying Vaccines And Viruses - Adsorptive media for chromatography, particularly ionexchange chromatography, derived from a shaped fiber, useful for purifying viruses. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Surface pendant functional groups can be added that provides ion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of viruses, such as influenza. | 12-10-2015 |
20160002605 | CHROMATOGRAPHIC PURIFICATION OF VIRUS PREPARATIONS WITH NEGATIVELY CHARGED PARTICLES - A method of purifying a sample that includes a desired virus includes the steps of (i) providing a packed chromatographic column having negatively charged porous particles, (ii) equilibrating the column to the conditions to which the desired virus in the sample is to elute, (iii) contacting the sample with the packed chromatographic column such that the sample volume applied to the packed chromatographic column is less than or equal to the interparticle space of the negatively charged porous particles within the packed chromatographic column, (iv) eluting the desired virus from the packed chromatographic column, where the desired virus is in a purer state and in the conditions to which the packed chromatographic column was equilibrated. | 01-07-2016 |
20160008788 | Methods And Materials For Microorganism Capture | 01-14-2016 |
20160024480 | RECOMBINANT AAV PRODUCTION IN MAMMALIAN CELLS - The present invention includes methods and compositions for the production of high titer recombinant Adeno-Associated Virus (rAAV) in a variety of mammalian cells. The disclosed rAAV are useful in gene therapy applications. Disclosed methods based on co-infection of cells with two or more replication-defective recombinant herpes virus (rHSV) vectors are suitable for high-titer, large-scale production of infectious rAAV. | 01-28-2016 |
20160032254 | AAV VECTORS PRODUCED BY INSECT CELLS COMPRISING REP52 AND REP78 CODING SEQUENCES WITH DIFFERENTIAL CODON BIASES - The present invention relates to production of proteins in insect cells whereby repeated coding sequences are used in baculoviral vectors. In particular the invention relates to the production of parvoviral vectors that may be used in gene therapy and to improvements in expression of the viral rep proteins that increase the productivity of parvoviral vectors. | 02-04-2016 |
20160040137 | SCALABLE PRODUCTION METHOD FOR AAV - A method for producing AAV, without requiring cell lysis, is described. The method involves harvesting AAV from the supernatant. For AAV having capsids with a heparin binding site, the method involves modifying the AAV capsids and/or the culture conditions to ablate the binding between the AAV heparin binding site and the cells, thereby allowing the AAV to pass into the supernatant, i.e., media. Thus, the method of the invention provides supernatant containing high yields of AAV which have a higher degree of purity from cell membranes and intracellular materials, as compared to AAV produced using methods using a cell lysis step. | 02-11-2016 |
20160060603 | FORMULATION OF SUGAR SOLUTIONS FOR CONTINUOUS ULTRACENTRIFUGATION FOR VIRUS PURIFICATION - The present invention provides a method for purification of a virus or virus antigen comprising providing a virus preparation and centrifugation of said virus preparation in a gradient of a sugar established by the addition of two or more buffered sugar layers of different concentration. The method leads to higher yields and reduces unwanted aggregation of the virus or virus antigen by increasing the volume of the peak pool. | 03-03-2016 |
20160068565 | METHOD FOR HARVESTING CULTURE PRODUCT - The present invention provides a more productive method for culturing and a more productive method for harvesting culture product in cell culture wherein the cell produces the culture product. The present invention relates to a method for harvesting a culture product contained in a culture solution in the cell culture wherein the cell produces the culture product, comprising the following steps: B sending the culture solution to a filtration membrane; C: filtering the culture solution by alternating tangential flow filtration while changing the flow of the culture solution so as to cause a reciprocating motion thereof in a direction parallel with the surface of the filtration membrane to obtain a filtrate; D: sending back a culture solution residue that has remained without permeating the filtration membrane; and G: harvesting the culture product from the filtrate, wherein
| 03-10-2016 |
20160090574 | A PROCESS FOR THE PRODUCTION OF ADENOVIRUS - The present disclosure relates to a process for the manufacture of adenoviruses wherein the process comprises culturing mammalian cells infected with the adenovirus in the presence of media suitable for supporting the cells such that the virus replicates, wherein the cells are capable of supporting viral replication, and at the end of the culturing period isolating from the media the adenovirus by filtering wherein the isolation of virus is not subsequent to a cell lysis step and to viruses obtainable from the process. | 03-31-2016 |
20160108367 | MDCK-DERIVED CELL STRAIN SUSPENSION-CULTURED IN PROTEIN-FREE MEDIUM AND METHOD FOR PROLIFERATING VIRUS USING CELL STRAIN - The present invention relates to a novel MDCK-derived cell line capable of being suspension-cultured in a protein-free medium and a method for proliferating a virus using the MDCK-derived cell line to produce a vaccine. The novel MDCK-derived cell line exhibits high and uniform productivity for various viruses, while causing less viral antigenic variations with low tumorigenicity, and thus can be useful in producing viruses used for vaccines. | 04-21-2016 |
20160145584 | MUTATED REP ENCODING SEQUENCES FOR USE IN AAV PRODUCTION - Nucleic acids encoding Parvoviral Rep proteins with a mutated nuclear localization signal (NLS) are provided. Also provided is a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein with a mutated zinc finger domain and a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein comprising an amino acid mutation at position 43, 57, 79, 97, 120, 179, 305, 484, 493 or 571 with reference to SEQ ID NO: 2. Nucleic acid constructs and cells, such as insect cells, comprising the nucleic acids are provided as well as a method for producing a recombinant Parvoviral virion using the nucleic acids. | 05-26-2016 |
20160152955 | METHOD FOR MANUFACTURING NON-ENVELOPED VIRUS | 06-02-2016 |