Class / Patent application number | Description | Number of patent applications / Date published |
435476000 | The polynucleotide is a plasmid or episome | 48 |
20080220528 | HIV GENE CLONING STRATEGY - The invention relates to methods for cloning Human Immunodeficiency Virus (HIV) genes, in particular HIV envelope genes. The invention also relates to cloning strategies for mapping resistance determinants for HIV genes, in particular HIV envelope genes. | 09-11-2008 |
20090004744 | MINICELLS AS VACCINES - The disclosed invention relates to immunogenic minicells cells (anucleated) and their use to induce an immune response from a subject. | 01-01-2009 |
20090035864 | PLASMIDS FOR TRANSFORMING BACTERIA OF THE ACIDITHIOBACILLUS SPP. GENUS, AND TRANSFORMATION METHOD - The present invention discloses functional plasmids in bacteria of the | 02-05-2009 |
20100167405 | PROCESSES FOR IMPROVED STRAIN ENGINEERING - Improvements in strain engineering technology are needed to insure the economic feasibility of future engineered recombinant organisms for industrial biotechnology. Disclosed herein are rapid, efficient methods (Genome Mass Transfer) that facilitate introduction of new selectable traits into a target microbial host. In one preferred embodiment, methods for high efficiency electroporation mediated transfer of donor DNA into a recipient microbial cell are disclosed. | 07-01-2010 |
20100197024 | APPARATUS AND METHOD FOR GENETICALLY TRANSFORMING CELLS - A fluid containing cells and free genetic material is acoustically coupled to a propulsion surface of a diaphragm. A blast-receiving surface of the diaphragm is acoustically coupled to an explosion chamber in which an explosive material is disposed. An ignition system ignites the explosive material in the explosion chamber to create a blast wave. The diaphragm transfers momentum from the blast wave to the fluid containing cells and free genetic material sufficient to cause the cells to take up the free genetic material. | 08-05-2010 |
20100210018 | MICROBIAL HOST-VECTOR COMPLEMENTATION SYSTEM - This invention provides a host-vector complementation system, which permits selection of vector-carrying host cells, without requiring an antibiotic resistance gene. In some embodiments, this system utilizes a host which is guaB deficient and vectors that carry and express the guaB gene. The invention also discloses methods of making and using the system. | 08-19-2010 |
20100233814 | Bacterial strains with improved plasmid stability - The present invention relates to the propagation of covalently closed circular recombinant DNA molecules such as plasmids, cosmids, bacterial artificial chromosomes (BACs), bacteriophages, viral vectors and hybrids thereof, and more particularly is strain modifications that improve strain viability, plasmid stability, plasmid production yield, and plasmid-directed protein production yield, using said DNA molecules in fermentation culture. | 09-16-2010 |
20120208281 | METHOD FOR INCORPORATION OF RECOMBINANT DNA - The invention provides methods of preparing bacteria of interest with a stably integrated nucleic acid sequence of interest. | 08-16-2012 |
20130280810 | METHOD FOR THE SELECTION OF RECOMBINANT CLONES COMPRISING A SEQUENCE ENCODING AN ANTIDOTE PROTEIN TO TOXIC MOLECULE - The present invention is related to a method for the selection of recombinant clones having integrated a gene of interest and a nucleotide sequence encoding a functional antidote protein to a toxic molecule, wherein said recombinant clones are the ones which survive following their integration into a host cell comprising in its genome a nucleotide sequence encoding said toxic molecule. The present invention is also related to a nucleic acid construct, a vector comprising said nucleic acid construct, a host cell and a cloning and/or sequencing kit for performing said method. | 10-24-2013 |
20140120623 | Method for Enhancing Cell Growth of Microalgae - Microalgae are potential energy resources for production of biofuels, such as biodiesel, ethanol, and butanol. A method for enhancing cell growth of microalgae enhances transgenic expression of a bicarbonate transporter (HCO | 05-01-2014 |
20140199767 | USE - The present invention relates to the use of one or more cas genes for modulating resistance in a cell against a target nucleic acid or a transcription product thereof. | 07-17-2014 |
20140335623 | METHODS AND COMPOSITIONS FOR GENERATING SPORULATION DEFICIENT BACTERIA - The present invention relates to methods and compositions for engineering sporulating bacterial cells, particularly a cell of the class Clostridia. In particular, the present invention relates to the generation of sporulation deficient bacteria for the generation of industrial superior phenotypes. | 11-13-2014 |
20150011008 | METHOD OF TRANSFERRING GENE INTO ALGAL CELL INVOLVING UTILIZING MULTIPLE SQUARE-WAVE PULSES IN THREE STEPS - To provide a technology that solves the above-mentioned problems, and is directly applicable to eukaryotic algal cells with cell-wall, the technology enabling gene transfer and transformation to be performed with high efficiency and good reproducibility irrespective of species of algae. Also provided is a method of transferring an exogenous gene into a eukaryotic algal cell, the method comprising performing electroporation using multiple square-wave pulses to the solution containing a cell of a green alga with cell-wall, and a nucleic acid molecule by the following three steps: applying a square-wave electric pulse (first electric pulse) with a high voltage for a short period of time under the condition that its total electric energy falls within a predetermined range; then applying a square-wave electric pulse (second electric pulse) with a low voltage for a long period of time two or more times; and then applying a square-wave electric pulse (third electric pulse) that is opposite in polarity to the second electric pulse, with a low voltage for a long period of time, two or more times. | 01-08-2015 |
435477000 | Plasmid or episome contains DNA targeting homologous recombination to bacteriophage, viral, or chromosomal DNA within a microorganism | 7 |
20100075424 | METHODS AND COMPOSITIONS FOR GENETICALLY ENGINEERING CLOSTRIDIA SPECIES - The present invention relates to methods and compositions for engineering Clostridia species. In particular, embodiments of the present invention relate to the expression of recombinant resolvase proteins in Clostridia species. | 03-25-2010 |
20110045594 | GENETIC REMODELING IN BIFIDOBACTERIUM | 02-24-2011 |
20130203170 | METHODS AND COMPOSITIONS FOR GENETICALLY MANIPULATING CLOSTRIDIA AND RELATED BACTERIA WITH HOMOLOGOUS RECOMBINATION ASSOCIATED PROTEINS - Methods for enhancing single cross-over homologous recombination in gram positive bacteria are presented. These methods provide enhanced capability to genetically modify gram positive bacteria. | 08-08-2013 |
20130280811 | PLASMIDS AND PHAGES FOR HOMOLOGOUS RECOMBINATION AND METHODS OF USE - Lambda phages that can be used to introduce recombineering functions into host cells are disclosed. Also disclosed are plasmids that can be used to confer recombineering functions to a variety of strains of | 10-24-2013 |
20140120624 | CASSETTES AND METHODS FOR TRANSFORMING AND SELECTING YEAST TRANSFORMANTS BY HOMOLOGOUS RECOMBINATION - A method for selecting a transformed yeast cell having integrated a nucleic acid fragment of interest by homologous recombination, cassettes and kits for carrying out the method are described. | 05-01-2014 |
20140154807 | METHOD FOR HOMOLOGOUS RECOMBINATION - The present invention discloses a method to construct eukaryotic cells having a target sequence in a chromosomal DNA sequence replaced by a replacement sequence of interest comprising:
| 06-05-2014 |
20140212977 | Methods For Producing Multiple Recombinant Polypeptides In A Filamentous Fungal Host Cell - The present invention relates to methods for constructing a filamentous fungal strain for production of multiple recombinant polypeptides having biological activity. The present invention also relates to methods for producing multiple recombinant polypeptides having biological activity in a filamentous fungal strain. The present invention also relates to filamentous fungal strains expressing multiple recombinant polypeptides having biological activity. | 07-31-2014 |
435478000 | Plasmid or episome contains at least part of a gene encoding a restriction endonuclease or modification enzyme | 4 |
20140120625 | Simultaneous Site-Specific Integrations of Multiple Gene-Copies in Filamentous Fungi - The invention relates to a method for the simultaneous integration of two or more copies of a polynucleotide of interest into the chromosome of a fungal host cell comprising at least two pairs of recognition sequences of a site-specific recombinase, each pair flanking a resident negative selection marker; transformation of the cell with a construct carrying a gene of interest also flanked by the recognition sequences to ensure double-crossover events after transient expression of the recombinase, followed by selection for excision of all negative selection markers from the cell. | 05-01-2014 |
20140186959 | Vectors for directional cloning - The invention provides vectors and methods for directional cloning. | 07-03-2014 |
20140315314 | ENGINEERED LISTERIA AND METHODS OF USE THEREOF - The invention provides a bacterium containing a polynucleotide comprising a nucleic acid encoding a heterologous antigen, as well as fusion protein partners. Also provided are vectors for mediating site-specific recombination and vectors comprising removable antibiotic resistance genes. | 10-23-2014 |
20160138030 | COMPOSITIONS AND METHODS FOR CLOSTRIDIAL TRANSFORMATION - The invention provides compositions and methods for clostridial bacteria that have been engineered to produce and/or to improve efficiency of production of industrial bioproducts. | 05-19-2016 |
435479000 | Plasmid or episome confers the ability to utilize directly a compound which a wild type microorganism is unable to utilize | 2 |
20120309093 | METHOD FOR PREPARING XYLOSE-UTILIZING STRAIN - A method for preparing a xylose-utilizing strain of | 12-06-2012 |
20140162367 | BACTERIAL STRAINS, PLASMIDS, METHOD OF PRODUCING BACTERIAL STRAINS CAPABLE OF CHEMOLITHOTROPHIC ARSENITES OXIDATION AND USES THEREOF - The invention provides novel strains | 06-12-2014 |
435480000 | Plasmid or episome contains at least part of a gene encoding a toxin or encoding for virulence or pathogenicity | 3 |
20100129916 | Expression of Toxic Genes In Vivo in a Non-Natural Host - Compositions and methods are provided for expression of a toxic protein in a host cell preferably a bacterial host cell where at least one T7 RNA polymerase gene Is contained on the host cell chromosome and one or more genes encoding a T7 RNA polymerase inhibitor is located on an F′ plasmid or on the chromosome. | 05-27-2010 |
20110171734 | CONJUGATIVE PLASMIDS AND METHODS OF USE THEREOF - The present invention provides novel | 07-14-2011 |
20140106458 | Conjugative Plasmids and Methods of Use Thereof - The present invention provides novel | 04-17-2014 |
435483000 | Yeast is a host for the plasmid or episome | 9 |
20100304490 | METHOD FOR GENERATING A GENETICALLY MODIFIED MICROBE - Provided herein are methods of generating genetically modified microorganisms, e.g., genetically modified yeast strains, which comprise functional disruptions in one or more pheromone response genes and one or more sporulation genes, and genetically modified yeast cells, e.g., genetically modified diploid and haploid yeast cells, that lack sporulation capability and endogenous mating capability, produced thereby. | 12-02-2010 |
20120100617 | PICHIA PASTORIS LOCI ENCODING ENZYMES IN THE ADENINE BIOSYNTHETIC PATHWAY - Disclosed are the ADE3, ADE4, ADE5, 7, ADE6, ADE8, ADE12, and ADE13 genes encoding various enzymes in the adenine biosynthesis pathway of | 04-26-2012 |
20120100618 | PICHIA PASTORIS LOCI ENCODING ENZYMES IN THE HISTIDINE BIOSYNTHETIC PATHWAY - Disclosed is the HIS7 gene encoding the His7p enzyme in the histidine biosynthesis pathway of | 04-26-2012 |
20120100619 | PICHIA PASTORIS LOCI ENCODING ENZYMES IN THE METHIONINE BIOSYNTHETIC PATHWAY - Disclosed are the MET1, MET3, MET4, MET6, MET7, MET8, MET10, MET14, MET16, MET17, MET19, MET22, MET2, and MET28 genes encoding various enzymes in the methionine biosynthesis pathway of | 04-26-2012 |
20120100620 | PICHIA PASTORIS LOCI ENCODING ENZYMES IN THE LYSINE BIOSYNTHETIC PATHWAY - Disclosed are the LYS1, LYS2, LYS4, LYS5, and LYS9 genes encoding various enzymes in the lysine biosynthesis pathway of | 04-26-2012 |
20120100621 | PICHIA PASTORIS LOCI ENCODING ENZYMES IN THE ARGININE BIOSYNTHETIC PATHWAY - Disclosed are the ARG5, 6, ARG8, ARG9, ARG80, ARG81, and ARG82 genes encoding various enzymes in the arginine biosynthesis pathway of | 04-26-2012 |
20120100622 | PICHIA PASTORIS LOCI ENCODING ENZYMES IN THE URACIL BIOSYNTHETIC PATHWAY - Disclosed are the URA1, URA2, URA4, and URA6 genes encoding various enzymes in the uracil biosynthesis pathway of | 04-26-2012 |
20140073054 | RECOMBINANT THERMOTOLERANT YEAST WITH A SUBSTITUTE HEAT SHOCK PROTEIN 104 PROMOTER - The invention provides a yeast strain and a method for making the same. The method has the step of replacing the regulation region upstream of the hsp104 gene in the genome of the yeast, so as to accelerate and prolong the expression span of hsp104 gene and enhance the capability of the yeast to ferment and produce ethanol in a high-temperature environment. The yeast is capable of fermenting glucose at a temperature higher than 42° C. to produce ethanol, or biomass ethanol, wherein the ethanol production ratio based on fermentation of glucose is higher than 97%. Being able to synchronize the degradation/hydrolysis stage and fermentation stage of biomass ethanol producing process, the yeast in accordance with the present invention is able to lower the production cost of biomass ethanol and further raise the productivity with its high ethanol production ratio. | 03-13-2014 |
20160002647 | PAN-YEAST AUTONOMOUSLY REPLICATING SEQUENCE - A DNA sequence that functions as an origin in many different yeast species. From 1 to 17 mutations can be introduced into this sequence to improve its function across multiple yeasts. The resulting synthetic DNA sequence confers stable plasmid replication function in all yeast species tested, including but not limited to | 01-07-2016 |
435484000 | Mycelial fungus is a host for the plasmid or episome | 3 |
20120276640 | Polypeptides Having Cellobiohydrolase Activity And Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 11-01-2012 |
20150307871 | METHOD FOR GENERATING SITE-SPECIFIC MUTATIONS IN FILAMENTOUS FUNGI - The present invention provides methods of making site-directed mutations in a gene encoding a polypeptide of interest to be transformed directly into a filamentous fungal host, without having to rely an intermediate host like | 10-29-2015 |
20190144877 | TRANSGENIC STRAIN CONTAINING AN ISOLATED PROMOTER | 05-16-2019 |
435485000 | Microorganism of the genus Bacillus is a host for the plasmid or episome | 3 |
20090098654 | Selectable genetic marker for use in pasteurellaceae species - The present invention provides a nucleic acid encoding nicotinamide phosphoribosyltransferase (NadV) from a V-factor independent bacterium and provides methods for using the gene as a selection marker for constructing recombinant bacteria from V-factor dependent bacteria. The method is an improvement over methods which rely on nucleic acids which confer antibiotic resistance for constructing recombinant bacteria. Methods for constructing attenuated recombinant | 04-16-2009 |
20110020938 | RECOMBINANT EXPRESSION PLASMID VECTOR AND RECOMBINANT STRAIN TO BE USED IN PRODUCING OXALATE DECARBOXYLASE, AND METHOD OF PRODUCING RECOMBINANT OXALATE DECARBOXYLASE - An object is to provide a means of highly producing an oxalate decarboxylase originating in a microorganism. A recombinant expression plasmid vector, which contains an α-amylase promoter belonging to the genus | 01-27-2011 |
20130210152 | Chloramphenicol Resistance Selection in Bacillus Licheniformis - The present invention relates to a modified | 08-15-2013 |
435487000 | Microorganism of the genus Brevibacterium or the genus Corynebacterium is a host for the plasmid or episome | 1 |
20090004745 | P19 Expression Units - The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms. | 01-01-2009 |
435488000 | Microorganism of the genus Escherichia is a host for the plasmid or episome | 2 |
20120238024 | Anti-Microbial Biotherapeutic Agents: Alternatives to Conventional Pharmaceutical Antibiotics - Novel antimicrobial agents that can serve as replacements to conventional pharmaceutical antibiotics are disclosed. The antimicrobial agents comprise conjugatively transmissible plasmids that kill targeted pathogenic bacteria, but are not harmful to donor bacteria. Two types of lethal transmissible plasmids are disclosed. One type kills recipient bacteria by unchecked (“runaway”) replication in the recipient cells and is prevented from occurring in donor cells. Another type kills recipient bacteria by expressing a gene that produces a product detrimental or lethal to recipient bacterial cells, that gene being prevented from expression in donor cells. | 09-20-2012 |
20130224865 | ANTI-MICROBIAL BIOTHERAPEUTIC AGENTS: ALTERNATIVES TO CONVENTIONAL PHARMACEUTICAL ANTIBIOTICS - Novel antimicrobial agents that can serve as replacements to conventional pharmaceutical antibiotics are disclosed. The antimicrobial agents comprise conjugatively transmissible plasmids that kill targeted pathogenic bacteria, but are not harmful to donor bacteria. Two types of lethal transmissible plasmids are disclosed. One type kills recipient bacteria by unchecked (“runaway”) replication in the recipient cells and is prevented from occurring in donor cells. Another type kills recipient bacteria by expressing a gene that produces a product detrimental or lethal to recipient bacterial cells, that gene being prevented from expression in donor cells. | 08-29-2013 |
435489000 | Plural nonidentical plasmids are introduced into a host microorganism or culture thereof (e.g., plasmid is part of a library, etc.) | 1 |
20130115702 | TRANSGENIC PHOTOSYNTHETIC MICROORGANISMS - Provided herein is a transgenic bacteria engineered to accumulate carbohydrates, for example disaccharides. Also provided is a photobioreactor for cultivating photosynthetic microorganisms comprising a non-gelatinous, solid cultivation support suitable for providing nutrients and moisture to photosynthetic microorganisms and a physical barrier covering at least a portion of the surface of the cultivation support. Devices for the large scale and continuous cultivation of photosynthetic microorganisms incorporating photobioreactors and methods of use are disclosed. Also disclosed are methods of producing fermentable sugar from photosynthetic microorganisms using a photobioreactor of the invention. | 05-09-2013 |