Entries |
Document | Title | Date |
20080199843 | Bioartificial Heart Tissue Graft And Method For The Production Therefor - The invention relates to a method for producing a bioartificial heart tissue graft, which leads to excellent biomechanical properties on the product and guarantees a high cell freedom with optimal preservation of the matrix. During the method, biological cells of a heart tissue preparation, particularly a heart valve or a heart vessel adhering in and/or to an extracellular matrix, are removed in-vitro. The method comprises that following steps carried out in this sequence: a) providing the heart tissue preparation of natural origin; b) removing cells, which are located in the tissue, from the extracellular matrix with the aid of an acellularization solution consisting of an aqueous solution of at least one strong anionic detergent and at least containing sodium deoxycholate; c) osmotically treating the tissue with distilled or deionized water, and; d) treating the tissue with a physiological saline solution with continuous flow or exchanging of the rinsing solution three times. | 08-21-2008 |
20080206733 | Method of Inducing Differentiation of Embryo-Stem Cell Into Hepatocyte and Hepatocyte Induced by the Method - With respect to a method for differentially inducing embryo-stem cells into hepatocytes, in order to obtain safe hepatocytes that are adequately functionable and able to supply in large quantity, a method for differentially inducing embryo-stem cell into hepatocyte, wherein the embryo-stem cells are cultured in the presence of deletion type hepatocyte growth factor is provided. Further, a method for differentially inducing embryo-stem cells into hepatocytes comprising (a) a step of forming the embryoid body of the embryo-stem cells and (b) a step of culturing the embryoid body in the presence of deletion type hepatocyte growth factor is provided. | 08-28-2008 |
20080206734 | HIGH SURFACE CULTIVATION SYSTEM WITH FILLER - An exemplary embodiment of a reversibly closable vessel suitable for the cultivation of cells and/or tissues can be provided. The exemplary vessel can comprise at least one reversibly closable aperture in the vessel wall, at least one wall provided in the interior of the vessel, with the wall being an integral part of the vessel. The interior can be divided into at least two compartments, whereas the wall comprises at least one of a hole, tube-like hole, opening, or a partially porous structure, which facilitates the flow of fluids from one compartment to another. At least one of the compartments can comprise at least one filler. | 08-28-2008 |
20080206735 | HIGH SURFACE CULTIVATION SYSTEM WITH SURFACE INCREASING SUBSTRATE - An exemplary embodiment of a culture vessel suitable is provided for a cultivation of cells and/or tissues. The exemplary vessel comprising at least one reversibly closable aperture in the vessel wall, and at least one surface-increasing substrate within the vessel, with the substrate being made of a single mold. According to another exemplary embodiment, a system can be provided comprising at least two vessels being interconnected via at least one aperture in their vessel wall, and a cultivation process using such a vessel or system, in which at least one type of cells, tissue, tissue-like cell cultures, organs, organ-like cell cultures, or multicellular organisms may be cultivated in the presence of at least one fluid or solid medium, e.g., provided for growing and/or cultivating the culture. | 08-28-2008 |
20080213742 | HIGH SURFACE CULTIVATION SYSTEM - An exemplary embodiment of a reversibly closable vessel suitable for the cultivation of cells and/or tissues can be provided. The exemplary vessel can comprise at least one reversibly closable aperture in the vessel wall, a convection arrangement inside the vessel which can comprise at least one blade. The arrangement can be configured to generate and/or modulate a convection in a fluid within the vessel when the vessel and/or the blade is agitated The convection arrangement and/or the blade can be at least particularly made from a porous material. | 09-04-2008 |
20080220406 | PHYSIOLOGICALLY ACCEPTABLE AQUEOUS SOLUTIONS AND METHODS FOR THEIR USE - Artificial plasma-like solutions and methods for their use are provided. The subject solutions include: electrolytes and an oncotic agent. The subject solutions find use in a variety of applications, particularly in those applications where at least a portion of a host's blood volume is replaced with a blood substitute. | 09-11-2008 |
20080233552 | METHOD OF CROSS-LINKING AMNION TO BE AN IMPROVED BIOMEDICAL MATERIAL - The present invention discloses a method of cross-linking amnion to be an improved biomedical material. The present invention adopts the amnion cross-linked by EDC (N-(3-dimethylaminopropyl)-N′-ethyl-carbodiimide HCI) or NHS (N-hydroxysuccinimide), and the cross-linked amnion not only has more resistance to protease, but also binds specific extracellular matrix (ECM) such as heparin by using cross-linked functional group. Further, by using the affinity of the ECM with specific growth factors, the amnion can be an efficient carrier for specific growth factor. Hence, some specific diseases may be treated. | 09-25-2008 |
20080268419 | Supplementation for Embryo and/or Cell Manipulation Media - The question is to increase the quality and safety of the media used in embryo and cell manipulations by means of supplementation, in the manipulation medium, by one or several of the following compounds: synthetic hyaluronan ( sHA), phospholipids or unsaturated fatty acids obtained from soybean seeds (PLFA), replacing others which are habitually added to embryo manipulation media and which produce potential damage and/or contamination by viruses, prions, endotoxins, etc., this being important with regard to the quality of the embryos or cells which it is desired to generate, both for the production of transgenic animals, for animal production, and for cell therapy or assisted reproduction; achieving a reduction in adhesiveness and an increase in viscosity, without losing the fluidity of the medium; this is essential in micro-manipulations such as ICSI, nuclear transfer, embryo biopsies, the micro-injection of cells into embryos in pre-implantational stages, or cell fusion. | 10-30-2008 |
20080286745 | Methods and Solutions for Tissue Preservation - Described herein are compositions and methods particularly useful in the medical arts. The compositions and methods may be used in connection with the preservation of a portion of a mammal, for example, tissues, organs, appendages, limbs, extremities, stem cells, myocytes, bone marrow, skeletal muscle as well as an array of other medical procedures, such as cardiac surgery, transplantation and/or preservation. In various embodiments, the inventive composition may be hyperoxygenated and be formulated to resemble the biochemistries of natural intracellular fluids. The inventive composition includes active ingredients to reduce ischemic, hypothermic and reperfusion injury during transplantation, thereby resulting in improved post-transplant graft function and quality, when used in connection with organ transplantation and storage procedures, for example cardiac transplantation. | 11-20-2008 |
20080286746 | PERFUSION APPARATUS AND METHOD - A perfusion apparatus for perfusing an organ includes a pump for pumping perfusion liquid though a perfusion liquid conduit, the conduit having an outlet. A pressure sensor senses the pressure of the perfusion liquid downstream of the pump and upstream of the outlet. A controller controls the output of the pump responsive to the pressure, so as to keep the pressure of the liquid reaching the organ substantially constant. | 11-20-2008 |
20080293034 | ORGAN ARREST, PROTECTION, PRESERVATION AND RECOVERY - The present invention relates to a composition for controlling viability of a tissue including a potassium channel opener or adenosine receptor agonist a compound for inducing local anaesthesia and a compound for reducing the uptake of water by a cell in the tissue. The present invention also relates to the use of the composition according to the invention for controlling viability of a tissue. | 11-27-2008 |
20080311552 | Use of Sirnas in Organ Storage/Reperfusion Solutions - The invention is the modification of organs, tissues and cells with a storage/reperfusion solution comprising siRNAs specific for genes whose expression is associated with loss of viability or cell damage. The presence of siRNAs in the storage/reperfusion solution minimizes and/or prevents organ, tissue and cell damage such that the organs, tissues or cells can be used for in vivo transplantation. The invention is also directed generally to methods for maintaining organs, tissues and cells in a viable state using the storage/reperfusion solution. | 12-18-2008 |
20080318202 | CHIMERIC PANCREAS - Novel methods, tissues and compositions for increasing the pancreatic mass of a mammalian recipient including harvesting immature pancreatic tissue from a mammalian donor and transplanting said tissue into the peritoneal cavity of a mammalian recipient under conditions that allow the pancreatic tissue to become vascularized and mature, thereby developing a functioning chimeric, endocrine pancreas that produces at least insulin in the recipient. The invention also includes mammalian immature pancreatic tissue adapted for transplantation into the peritoneal cavity of a mammalian recipient for increasing the pancreatic mass of the mammalian recipient as well as methods and compositions for treatment of the pancreatic tissue, recipient immunosuppression and recipient co-stimulatory blockade. | 12-25-2008 |
20090011399 | METHOD AND APPARATUS FOR BIOPSY SAMPLE PROCESSING - A container for use in processing a biopsy sample. Biopsy sample and materials are received within a sample processing area of the container. A semi-permeable barrier is positioned at a downstream portion of the sample processing area and prevents the prevent passage of the biopsy sample. A cap is positioned at the downstream side of the barrier, and holds fluids within the container. | 01-08-2009 |
20090017437 | FIXATIVE COMPOSITION - The invention relates to a fixative composition for preservation of tissue and biological samples. Current fixative compositions have the drawback that they do not sufficiently protect against DNA/RNA degeneration. In addition their use impairs extractability and compromises amplifiability of extracted DNA. The invention solves the combined but related problems and provides a fixative composition comprising one or more alkanols, polyethylene glycol having a molecular weight of 200-600, one or more weak organic acids in a combined concentration of 0.01 to 0.10 mole per liter of the fixative composition, and water. The fixative composition is essentially free of any cross-linking agents such as formaldehyde. | 01-15-2009 |
20090017438 | METHODS AND COMPOSITIONS FOR THE PRESERVATION OF CELLS AND TISSUES - Disclosed are methods for the preservation and storage of living biological tissues, organs, and populations of isolated cells. Also disclosed are compositions and methods to permit biological samples (including e.g., cells, cell cultures, tissues, and organs) to be harvested from suitable donor animals, stored for prolonged periods under refrigerated, cryogenic, or near-freezing, and then transported and implanted into a site within the body of a selected recipient animal, all without significant loss of cellular viability, tissue integrity, and/or biochemical function of the stored biological sample. | 01-15-2009 |
20090017439 | COMPOSITIONS AND METHODS FOR ENHANCING THE VIABILITY OF ANIMAL CELLS, TISSUES, AND ORGAN EXPLANTS - Disclosed are compositions and methods for the preservation, storage, and transport of living biological tissues, organs, and populations of isolated cells. In particular, the disclosed compositions and processes permit mammalian cells, tissues, and organs to be harvested from suitable donor animals, stored for prolonged periods, and transported to the site of recipient implantation, all without significant loss of cell viability, biological activity, and/or tissue integrity. | 01-15-2009 |
20090061410 | Multiloop Engineered Heart Muscle Tissue - The invention is directed to a method for the preparation of a multiring engineered heart tissue construct suitable for use in cardiac tissue augmentation and/or replacement therapy. The invention further refers to multiring EHT constructs which comprise at least two force-generating engineered heart tissue rings fused with each other and a device for preparing the same. Finally, the invention relates to force-generating engineered heart tissue rings derived from human cells and their use in drug screening and target validation assays. | 03-05-2009 |
20090081631 | Tissue Engineered Organ - Tissue engineered organs and methods for producing the same are provided. The tissue engineered organs are useful to replace diseased or damaged host organs. | 03-26-2009 |
20090104593 | Preparation and Use of Basement Membrane Particles - A method for supporting the in vitro growth of one or more eukaryotic cell type(s), the method comprising; seeding cells of said cell type(s) onto particles comprising basement membrane or a basement membrane-like substrate, and culturing said seeded cells in vitro under conditions suitable for expansion of said seeded cells, wherein said particles are less than about 500 μm in size. | 04-23-2009 |
20090123904 | COMPOSITION FOR PRESERVING CELLS, TISSUES, OR ORGANS, PRODUCTION PROCESS AND USES - The object of the invention is a composition for protecting and/or preserving cells, tissues or organs, characterized in that it comprises at least one cyclodextrin, preferably closely combined with an antioxidant. | 05-14-2009 |
20090136912 | CARRIER FOR CELL CULTURE - A carrier for cell culture is provided which improves the cell proliferativity in serum-free culture and which is free from risk from infection factor contamination. The gist of the features of the present invention is to be formed of a crosslinked poly (meth) acrylic acid (salt) particle (A) and an artificial polypeptide (P) having at least one cell-adhesive minimal amino acid sequence (X) in one molecule and to have a water retention value of from 2 to 50 g/g. The (A) is preferably a particle produced by reversed phase suspension polymerization of an aqueous monomer solution containing (meth)acrylic acid and/or an alkali metal salt of (meth)acrylic acid. The (P) preferably has at least one auxiliary amino acid sequence (Y) in one molecule of the (P). The (X) is preferably an Arg Gly Asp sequence. | 05-28-2009 |
20090155760 | Method of Cell Injection into Biotissue and Apparatus Therefor - A method of injecting cells into a biological tissue comprising thrusting injection needle ( | 06-18-2009 |
20090186332 | PREPARATION AND STORAGE OF STABLE, ANTIMICROBIALLY ACTIVE MATERIALS - A method for the preparation of antimicrobially active materials is presented. The invention involves taking a base material such as allografts, xenografts, polymers, metals, and ceramics and combining it with an antimicrobially active agent, such as antibiotics, antibacterials, antifungals, antivirals, disinfectants, and polypeptides, after which it is irradiated with ionizing radiation to sterilize and stabilize the combined material. The resulting antimicrobially active material may then be stored at ambient temperature while maintaining its antimicrobial activity and the structural integrity of the base material. The invention is particularly useful for both preventing and treating a variety of infections and for increased safety in reconstructive procedures. | 07-23-2009 |
20090186333 | Cyclic Implant Perfusion, Cleaning and Passivation Process and Implant Produced Thereby - This invention is a novel method for perfusion of a porous implant which achieves efficient interpenetration of desired factors into and removal of undesirable factors from the pores of the implant, cleaning of the implant, efficient passivation of the implant (inactivation of pathogens, microorganisms, cells, viruses and the like and reduction in antigenicity thereof), and the novel implant produced by such treatment. The process presents a system wherein the rate of pressure cycling, the fact of pressure cycling, and the amplitude of pressure cycling, results in highly cleaned tissues and other implants for implantation. Target decontamination goals for this process include between about a one (1) to twelve (12) log reduction in bacterial contamination, between about a one (1) to fifteen (15) log reduction in enveloped virus contamination, up to about a five (5) log reduction in non-enveloped virus contamination, between about a two (2) to ten (10) fold reduction in endotoxin, maintenance of implant or graft biologic and biomechanical properties, absence of tissue toxicity due to cleaning solutions used, and reduced implant antigenicity. | 07-23-2009 |
20090191533 | Process for Preserving Tissues - A process for preserving tissues is described which comprises transferring the tissues in a container and evacuating the container. | 07-30-2009 |
20090191534 | SYSTEM AND SEQUENTIAL CULTURE MEDIA FOR IN VITRO FERTILIZATION - Instead of immersing human reproductive cells in a single culture medium throughout the various procedures used in IVF, a process is provided by which the reproductive cells may be moved through a sequence of distinct culture media as the various IVF procedures are carried out. In one implementation, the culture media specifically formulated to provide a physical environment similar to that found within the female reproductive tract and conducive to growth and development of human reproductive cells during the various stages of the IVF process. In this regard, specifically formulated culture media can be applied to support the reproductive cells in one or more of the following procedures: oocyte retrieval and handling; oocyte maturation; ordinary fertilization; oocyte, zygote and embryo examination and biopsy; embryonic development to the eight-cell stage; embryonic development to the blastocyst stage; embryo transfer, and cryopreservation. | 07-30-2009 |
20090202976 | AMNIOPUNCH AND USES THEREOF - A tissue, such as an amniotic or chorionic membrane, harvesting device that integrates a system facilitating subsequent storage of tissue samples. The device cuts a sample of the target tissue and automatically deposits the sample in a storage vessel. A method of collecting and storing a sample from a target tissue using a tissue harvesting device. A kit for collecting and storing samples of the target tissue. | 08-13-2009 |
20090239206 | SOLUTION FOR PRESERVING LIVER - The present invention provides a liver preservation solution containing trehalose and dibutyryl-cAMP. The content of nitroglycerin in the preservation solution is preferably lower than 0.44 mM. In the liver preservation solution of the present invention, since the toxicity due to nitroglycerin, which is observed during liver preservation, has been improved, liver transplantation can be performed with a high engrafted rate. | 09-24-2009 |
20090258337 | Thawed organ or tissue or thawed cell group to be donated, transplanted, added, or administered to living body, production process thereof, supercooled solution therefor, and production apparatus of the organ or tissue - The present invention aims to freeze and store organs or tissues taken from human bodies or animals and after thawing, use the resulting organs or tissues, or cells taken and extracted therefrom for medical treatment. The present invention is characterized in that the organs or tissues taken from human bodies or animals are regenerated through the steps of freezing, storing and thawing; the cells taken from the organs or tissues undergo cell division and colonization. | 10-15-2009 |
20090275011 | SESSILE STEM CELLS - Sterile, virally safe, heterologous, homologous, isologous or autologous tissue, tissue-typed or not tissue-typed, which contains predifferentiated and/or differentiable sessile stem cells and which can be used for wound closure and/or promotion of wound healing. | 11-05-2009 |
20090280469 | CELL MASS CAPABLE OF SERVING AS A PRIMITIVE ORGAN-LIKE STRUCTURE COMPRISED OF A PLURALITY OF CELL TYPES OF SOMATIC ORIGIN - The present invention provides a method of producing a cell mass capable of serving as a primitive organ-like structure comprised of a plurality of somatic cell types of somatic origin, comprising: preparing cultures containing the plurality of types of somatic cells; mixing the plurality of types of somatic cell cultures followed by adding a Wnt signal activator to the mixed cell culture; subjecting the culture containing the Wnt signal activator to non-plate contact culturing over a predetermined time period; and replacing the medium of the culture cultured by the non-plate contact culturing with medium not containing Wnt signal activator and further culturing for a predetermined time period; wherein, at least one type of the plurality of somatic cells is maintained in an undifferentiated state. | 11-12-2009 |
20090298042 | THREE-DIMENSIONAL SKIN MODEL - The present invention relates to methods for cultivating dermal fibroblasts, methods for preparing in vitro dermis equivalents, methods for preparing three-dimensional in vitro skin equivalents, an in vitro dermis equivalent, a three-dimensional in vitro skin equivalent, and methods for determining the effect of a chemical substance or of an agent on human skin cells using the in vitro dermis equivalent and/or the in vitro skin equivalent. | 12-03-2009 |
20090311662 | Method for Harvesting and Preparing Porcine Hearts for Use in a Cardiac Surgical Simulator - A method is described and disclosed for harvesting and preparing porcine hearts that may be used, among other things, as an improved analog of the human heart in cardiac simulator systems for training surgical personnel for performing beating heart surgery. | 12-17-2009 |
20100028848 | Compositions and Methods of Using siRNA to Knockdown Gene Expression and to Improve Solid Organ and Cell Transplantation - This invention describes compositions and methods using siRNA to target various genes expressed in cells of transplanted organs or tissues and/or genes expressed in the host to improve the success of the transplantation. | 02-04-2010 |
20100068690 | TISSUE PRESERVATION AND FIXATION METHOD - This invention relates, e.g., to a composition that, at room temperature, when contacted with a sample comprising phosphoproteins, can fix and stabilize cellular phosphoproteins, preserve cellular morphology, and allow the sample to be frozen to generate a cryostat frozen section suitable for molecular analysis. The composition comprises (1) a fixative that is effective to fix the phosphoproteins, and that has a sufficient water content to be soluble for a stabilizer and/or a permeability enhancing agent); (2) a stabilizer, comprising (a) a kinase inhibitor and (b) a phosphatase inhibitor and, optionally, (c) a protease (e.g., proteinase) inhibitor; and (3) a permeability enhancing agent (e.g. PEG). Methods are described for preserving phosphoproteins, using such a composition. Also described are endogenous surrogate markers for monitoring protein degradation, including the loss of posttranslational modifications (such as phosphorylation), e.g. the following removal of a cell or tissue from a subject; and exogenous molecular sentinels (e.g. phosphoproteins attached to magnetic nanoparticles) that allow one to evaluate the processing history of a cellular or tissue population sample. | 03-18-2010 |
20100086907 | FLUIDICS BASED PULSATILE PERFUSION PRESERVATION DEVICE AND METHOD - In one embodiment, a preservation apparatus is described that includes a storage compartment. The storage compartment is configured to hold an organ or tissue and a preservation fluid. A cover assembly is configured to engage the storage compartment. The cover assembly includes a support element, wherein the support element together with the storage compartment define a storage chamber. The cover assembly also includes a lid and a gas permeable membrane disposed between the lid and the support element. The gas permeable membrane and the support element together define a perfusion chamber configured to hold preservation fluid and an organ or tissue during use. | 04-08-2010 |
20100092938 | Novel and Practical Serological Assay for the Clinical Diagnosis of Leishmaniasis - Methods for the diagnosis of visceral, cutaneous and canine leishmaniasis in a subject suspected of being infected with the parasitic protozoa | 04-15-2010 |
20100120013 | PROCEDURE FOR LONG TERM CORNEAL CULTURE - A method for long-term multi-species cornea culture and preservation is provided. Excised mammalian corneas are preserved with structural integrity by filling the endothelial cavity with a plug material. Plugged corneas are sterile-cultured for more than 21 days via an air-interface culture where nutrients from a culture media are supplied to corneal tissue primarily through the plug. Plugged corneas are incubated in culture dishes with a customized media continuously in contact with the ocular sclera and corneal plug, and periodically bathing the corneal surface epithelia. An animal-alternative toxicology assay is also provided using excised porcine corneas capable of assessing ocular injury reversibility within 21 days. Further provided is a method of mammalian corneal preservation for excised human corneas extending their storage life to three to four weeks. | 05-13-2010 |
20100120014 | Stability Drying - A method of formulating high ambient temperature (room temperature and above) stable biologics (biologically active macromolecules, enzymes, serums, vaccines, viruses, pesticides, drug delivery systems, liposomes, cells suspensions, sperm, erythrocytes, other blood cells, stem cells, multicellular tissues, skin, heart valves) including secondary drying comprising at least two steps of stability drying at elevated temperature: 35° C., 40° C., 45° C., 50° C., and higher temperatures. The method could be applied to stabilize biologics encapsulated in alginate gel microspheres for better oral delivery. The method encompasses the following: microspheres are formulated using a cryo-encapsulation procedure comprised of mixing drops of frozen preservation mixture (To form the preservation mixture, biologics are mixed with preservation solutions containing sodium alginate.) with frozen drops of a calcium solution (i.e. calcium gluconate) and subsequent warming to form the gel particles. | 05-13-2010 |
20100143876 | TRANSPLANT MEDIA - The present invention relates to media containing purified antimicrobial polypeptides, such as defensins, and/or cell surface receptor binding proteins. The media may also contain buffers, macromolecular oncotic agents, energy sources, impermeant anions, ATP substrates. The media find use for the storage and preservation of internal organs prior to transplant. | 06-10-2010 |
20100151435 | COMPOSITIONS AND METHODS FOR TISSUE PRESERVATION - Methods and compositions for resuscitating, storing, and preserving functional integrity of organs and tissues. Metabolic function is maintained by sustaining ATP levels, mitochondrial function, cardiomyocyte contractility, prevention of acidosis, inhibition of induction of apoptosis, maintaining ionontrophy and lusiotrophy by regulating calcium, sodium, potassium and chloride ions. | 06-17-2010 |
20100151436 | Methods for Ex Vivo Administration of Drugs to Grafts Using Polymeric Nanoparticles - Methods for ex vivo administration of drugs to grafts using polymeric micro- and nanoparticles and applications for these methods are described herein. The particles contain encapsulated molecules which are released locally at the site of implantation and function to prevent graft rejection or aid in the proper adaptation of the graft to the host. The disclosed methods may be used to inhibit or reduce hyperplasia and stenosis of vascular grafts or to prevent graft rejection. | 06-17-2010 |
20100167259 | APPARATUS AND METHOD FOR THE PRESERVATION OF PANCREATIC TISSUE AND ISLET CELLS FOR TRANSPLANTATION - The present invention includes compositions and methods for the preparation, preservation and storage of organs (e.g., pancreatic islet cells) for transplantation by storing organs in which the organs are suspended in a solution for maintaining viability and the organ or cells are cooled in a refrigeration unit for the entire duration of storage in which the average temperature in the apparatus does not vary by more than 2 degrees centigrade from the set temperature. | 07-01-2010 |
20100173277 | Agent for Suppressing Rejection in Organ Transplantation Comprising Anti-HMGB-1 Antibody - An objective of the present invention is to provide effective methods for suppressing rejection in organ transplantation, in particular, pancreatic islet transplantation which is useful in treating diabetes. The present invention demonstrated that antibodies against HMGB-1 suppressed the rejection in pancreatic islet transplantation and promoted the survival of grafted pancreatic islets. Thus, the present invention provides agents that comprise an anti-HMGB-1 antibody for suppressing rejection in organ transplantation. | 07-08-2010 |
20100196870 | STERILIZED XENOGRAFT TISSUE - The invention provides an article of manufacture comprising a substantially non-immunogenic xenograft for implantation into humans. The invention also provides methods for preparing a xenograft by removing at least a portion of a soft tissue from a non-human animal to provide a xenograft; washing the xenograft in saline and alcohol; subjecting the xenograft to cellular disruption treatment; treating the xenograft with crosslinking agents, and digesting the xenograft with a proteoglycan-depleting factor and/or glycosidase. The invention further provides a method for sterilizing xenograft material, having the steps of obtaining substantially non-immunogenic xenograft material; treating the xenograft material with at least one crosslinking agent; and subjecting the crosslinked xenograft material to radiation treatment. | 08-05-2010 |
20100196871 | APPARATUS AND METHODS FOR CULTURING AND/OR TRANSPORTING CELLULAR STRUCTURES - An apparatus for culturing and/or transporting embryos, oocytes or other cellular structures, comprising a housing ( | 08-05-2010 |
20100216109 | METHOD AND APPARATUS FOR DRUG DELIVERY TO TISSUE OR ORGAN FOR TRANSPLANT - It is intended to provide a method for delivering a drug such as a gene to a tissue or organ for transplant such as skin by use of a laser-induced stress wave (LISW) and thereby producing a high-performance tissue or organ for transplant with a high survival ability and to provide an apparatus for carrying out the method as well as a high-performance tissue or organ for transplant produced by the method. The present invention provides a method for delivering a drug to a graft of a tissue or organ for transplant, comprising applying a drug to a graft of a tissue or organ for transplant and applying, to the graft, a stress wave induced by the laser light irradiation of a light absorber provided in proximity to the graft, wherein the light absorber is made of a substance capable of absorbing a laser light and generating a stress wave. | 08-26-2010 |
20100233669 | BIOBURDEN-REDUCING ANTIBIOTIC COMPOSITION AND METHOD OF USE - An antibiotic composition is provided for use in decontaminating biological tissue, for example, graft tissues for transplantation or preparation for terminal sterilization. The antibiotic composition is a physiological solution includes a lantibiotic, such as nisin. The antibiotic solution is effective to substantially inhibit bacterial growth of at least one type of gram-positive bacteria while substantially maintaining the physiological characteristics of the tissue. The method may include contacting the tissue with the antimicrobial composition at a temperature and for a period of time effective to substantially inhibit bacterial growth of the at least one type of gram-positive bacteria while substantially maintaining the physiological characteristics of the tissue. | 09-16-2010 |
20100248205 | Reovirus Clearance of Ras-Mediated Neoplastic Cells from Mixed Cellular Compositions - Reovirus can be used to selectively remove ras-mediated neoplastic cells from a cellular composition. It is of particular interest to purge autographs which may contain neoplastic cells with reovirus before transplanting the autographs back into the recipient, thereby reducing the risk of introducing or reintroducing neoplastic cells into the recipient. | 09-30-2010 |
20100255457 | THIN FIBRIL COLLAGEN MATERIAL AND METHOD FOR MAKING THIN FIBRIL COLLAGEN FROM NATIVE COLLAGEN FIBERS - A collagen material having a form of thin fibrils generally free of fibril-bundling proteogylcan interactions, and a method for providing the thin fibril collagen material from native collagen fibers. The method uses proteoglycan antibodies to disassociate the proteoglycan interactions in bundled collagen fibrils to provide the constituent fibrils. The process can be used as a model for arthritis and the resulting fibrils can be used to form new extracellular matrix biomaterials and new tissues. | 10-07-2010 |
20100267002 | PEPTIDE HAVING LIFE-LENGTHENING EFFECT ON CELLS - This invention provides a substance that exhibits excellent life-lengthening effects on cells, that is excellent in terms of productivity, and that has low immunological toxicity. The present invention relates to a preservative for a biomaterial comprising (a) a peptide consisting of the amino acid sequence as shown in SEQ ID NO: 6 or (b) a peptide consisting of an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO: 6 by deletion, substitution, insertion, or addition of 1 or several amino acids and having life-lengthening effects on cells and a method for preserving a biomaterial utilizing such peptide. | 10-21-2010 |
20100291532 | PROCESS FOR STERILIZING ACELLULAR SOFT TISSUE WITH IRRADIATION - The present invention relates to a process for preparing skin removed from a human donor, removal of cellular components and sterilizing the decellularized skin. The process comprises the following steps:
| 11-18-2010 |
20100291533 | INDOLE AND INDAZOLE DERIVATIVES HAVING A CELL-, TISSUE- AND ORGAN-PRESERVING EFFECT - The present invention relates to a composition for preserving cells, tissues and organs, comprising as an active ingredient indole and indazole compounds of formula (1), or a pharmaceutically acceptable salt or isomer thereof, which are effective for preventing injury of organs, isolated cell systems or tissues caused by cold storage, transplant operation or post-transplantation reperfusion; a preservation method; and a preparation method of the composition. | 11-18-2010 |
20100297599 | TRANSPARENT COOLING GEL - For cooling organs and tissues in transplantation medicine, only ice preparations are used so far which are not soft like a gel and are brought to the necessary degree of comminution by mechanically comminuting frozen, sterile, pyrogen-free isotonic infusion solutions, such as 5% glucose or 0.9% saline, with great effort. The lack of gel-like consistency of the preparations and the expensive production process are very disadvantageous. | 11-25-2010 |
20110008762 | Methods for Preserving Organs and Tissues - The invention relates to a method for preserving an organ or tissue comprising contacting the organ or tissue with an effective amount of a kallikrein inhibitor and solutions useful for such a method. Also provided is a method for reducing reperfusion injury of an organ during surgery and/or following removal of the organ from a subject comprising placing the organ in an organ storage and preservative solution, wherein the solution comprises a kallikrein inhibitor. | 01-13-2011 |
20110065083 | Compositions and Methods for Enhancing the Viability of Animal Cells, Tissues, and Organ Explants - Disclosed are compositions and methods for the preservation, storage, and transport of living biological tissues, organs, and populations of isolated cells. In particular, the disclosed compositions and processes permit mammalian cells, tissues, and organs to be harvested from suitable donor animals, stored for prolonged periods, and transported to the site of recipient implantation, all without significant loss of cell viability, biological activity, and/or tissue integrity. | 03-17-2011 |
20110076665 | ELECTROMAGNETIC CONTROLLED BIOFABRICATION FOR MANUFACTURING OF MIMETIC BIOCOMPATIBLE MATERIALS - The precise application of an electromagnetic field controls cell motion to guide extrusion and deposition of biopolymers produced by the cells. This controlled biofabrication process is used to fabricate two- and three-dimensional networks of biocompatible nanofibrils (such as cellulose) for use as biomaterials, tissue scaffolds to be used in regenerative medicine, coatings for biomedical devices, and other health care products. | 03-31-2011 |
20110091862 | GENERATION OF AN HLA-NEGATIVE OSTEOGENIC PRECURSOR CELL LINE - The present disclosure provides methods for producing human osteogenic cell lines in which HLA Class I expression is absent or reduced. The resulting osteogenic cells can then be used, for example, to form mineralized three-dimensional bone constructs which can be used, for example, in bone grafting procedures. The lack of HLA Class I expression in the cells of the mineralized three-dimensional bone constructs reduces or eliminates the potential for host-graft rejection. | 04-21-2011 |
20110097700 | Methods for reducing levels of pro-inflammatory or anti-inflammatory stimulators or mediators in the blood - Devices, systems, and methods reduce levels of pro-inflammatory or anti-inflammatory stimulators or mediators in blood by selective adsorption. The devices, systems, and methods are useful in situations where abnormal levels of or unregulated or excessive interaction among pro-inflammatory or anti-inflammatory stimulators or mediators occur, or during events that do induce or have the potential for inducing abnormal production of pro-inflammatory or anti-inflammatory stimulators or mediators. The devices, systems, and methods serve to prevent, control, reduce, or alleviate the severity of the inflammatory response and disease states that are associated with abnormal levels of or unregulated or excessive interaction among pro-inflammatory or anti-inflammatory stimulators or mediators. | 04-28-2011 |
20110143331 | Methods and Compositions for the Preservation of Cells and Tissues - Disclosed are methods for the preservation and storage of living biological tissues, organs, and populations of isolated cells. Also disclosed are compositions and methods to permit biological samples (including e.g., cells, cell cultures, tissues, and organs) to be harvested from suitable donor animals, stored for prolonged periods under refrigerated, cryogenic, or near-freezing, and then transported and implanted into a site within the body of a selected recipient animal, all without significant loss of cellular viability, tissue integrity, and/or biochemical function of the stored biological sample. | 06-16-2011 |
20110165553 | VITRIFIED COMPOSITION WHICH PRESERVES BIOLOGICAL MATERIALS - The present invention relates to a method for preserving biological material comprising the steps of: providing a vitrification solution comprised of the biological material and a vitrification agent where the solution has a temperature in the range from 0.1° C. to 17.9° C.; microwaving the vitrification solution for a first period of time; allowing the vitrification solution to rest for a second period of time; repeating steps b and c until the vitrification solution enters into a glassy state. | 07-07-2011 |
20110171624 | Media solutions and methods for cryopreservation and thawing of in vitro fertilization specimens - A medium solution which will increase the growth, survival and ultimately the live birth rate of oocytes and embryos which have been or will be subjected to cryopreservation. The solution contains varied amounts of glucose, pyruvates, amino acids, vitamins K5 and C, antioxidants, fatty acids to supply the specimens with the chemical ingredients and uptake requirements required to recover and prosper during and after the cryopreservation process. The solution supplies nutrients to the specimens that will replenish depletion and damage to the specimens and their mitochondria, spindles and structural features, such as cell walls. One formulation addresses the additional requirements of frozen specimens as opposed to the current media solutions and methods which treat the un-frozen specimens the same as the frozen specimens when recovering them from cryopreservation. | 07-14-2011 |
20110171625 | Media solutions and methods for cryopreservation and thawing of in vitro fertilization specimens - A medium solution which will increase the growth, survival and ultimately the live birth rate of oocytes and embryos which have been or will be subjected to cryopreservation. The solution contains varied amounts of glucose, pyruvates, amino acids, vitamins K5 and C, antioxidants, fatty acids to supply the specimens with the chemical ingredients and uptake requirements required to recover and prosper during and after the cryopreservation process. The solution supplies nutrients to the specimens that will replenish depletion and damage to the specimens and their mitochondria, spindles and structural features, such as cell walls. One formulation addresses the additional requirements of frozen specimens as opposed to the current media solutions and methods which treat the un-frozen specimens the same as the frozen specimens when recovering them from cryopreservation. | 07-14-2011 |
20110200981 | Polymerized Hemoglobin Media And Its Use In Isolation And Transplantation of Islet Cells - Solutions and suspensions comprising polymerized hemoglobin derived from human blood are disclosed. The solutions and suspensions may comprise cell culture medium, an enzyme (such as a protease), and/or a buffer. Processes of preparing the solutions and suspensions are also disclosed. The solutions and suspensions may be employed in methods of isolating mammalian cells, such as pancreatic islets, methods of preserving mammalian tissue and organs, methods of aiding the recovery of mammalian cells following their isolation, methods of maintaining mammalian cells, methods of propagating mammalian cells, and methods of treating a mammal with diabetes. | 08-18-2011 |
20110236874 | METHOD FOR FORMATION OF RENAL TUBULES - There is provided a method of making a renal tubule. The method comprises seeding renal tubule cells onto a solid surface; culturing the renal tubule cells in a liquid growth medium to form a monolayer on the solid surface; and continuing culturing the renal tubule cells to form a tubule. | 09-29-2011 |
20110287403 | Graft Tensioning Device - A graft tensioning device comprising a basin capable of containing a fluid, a heating element capable of heating the fluid to an appropriate temperature, a tray disposed within the basin, and a graft holding fixture for maintaining a tissue graft in tension, permanently or removably secured to the tray, wherein the graft holding fixture and tissue graft may be submersed within the fluid. The device may further comprise a base, a shelf and/or a cover. The device may be reusable, disposable or some combination thereof. | 11-24-2011 |
20110287404 | Stationary Bubble Reactors - Reactors that allow mixing and gasification by converting the entire floor of the reactor vessel to a sparge filter is described. | 11-24-2011 |
20110311959 | SOLUTION FOR PRESERVING LIVER - The present invention provides a liver preservation solution containing trehalose and dibutyryl-cAMP. The content of nitroglycerin in the preservation solution is preferably lower than 0.44 mM. In the liver preservation solution of the present invention, since the toxicity due to nitroglycerin, which is observed during liver preservation, has been improved, liver transplantation can be performed with a high engrafted rate. | 12-22-2011 |
20120028234 | TISSUE AND ORGAN GRAFT BIOREACTOR AND METHOD OF OPERATION - A single-use, single or multiple tissue, organ, and graft bioreactor and environmental control system is designed to replicate the necessary conditions for growth of tissues, organs, or grafts, while addressing problems in scaling up the tissue growth; adaptation to a single-use or disposable format; and operation as a stand-alone unit that provides full environmental control of cell culture conditions. | 02-02-2012 |
20120034593 | CYCLIC PEPTIDES WITH AN ANTI-PARASITIC ACTIVITY - The present invention relates to a method for preparing a cyclic peptide with antiparasite activity and anticancer activity. The invention also relates to this peptide as an antiparasite agent, for example in the treatment of toxoplasmosis and as an anticancer agent. The invention also relates to the use of this cyclic peptide for treating organs ex vivo before transplantation. | 02-09-2012 |
20120135390 | PLASMA TREATMENT FOR GROWTH FACTOR RELEASE FROM CELLS AND TISSUES - Aspects of the present invention are related to methods comprising contacting an endothelial cell in an endothelial cell population with a non-thermal plasma to release an angiogenic growth factor. The released angiogenic growth factor may induce endothelial cell proliferation. In certain embodiments, the angiogenic growth factor is fibroblast growth factor-2. Preferably, the non-thermal plasma may be an atmospheric pressure dielectric barrier discharge. Additional aspects of the present invention are directed to methods for treating a disease comprising promoting angiogenesis by contacting an endothelial cell in a endothelial cell population with a non-thermal plasma to release an angiogenic growth factor. The angiogenic growth factor may induce endothelial cell proliferation. Further aspects of the present invention are directed to methods for treating a disease comprising inhibiting angiogenesis by contacting an endothelial cell in a endothelial cell population with a non-thermal plasma to reduce the number of endothelial cells in the population. | 05-31-2012 |
20120141973 | PERFUSION AND/OR PRESERVATION SOLUTION FOR ORGANS - The present invention relates to a solution for preservation, perfusion, and/or reperfusion of an organ, especially the heart, for transplantation. The solution contains peptide inhibitor(s) of protein kinase C βII (PKC βII) and/or of protein kinase C ζ (PKC ζ) and/or peptide activator(s) of protein kinase C δ (PKCδ). Methods for using the inventive solution are also disclosed, including methods for preserving an organ for transplantation, for protecting an ischemic organ from damage, for attenuating organ dysfunction after ischemia, for maintaining nitric oxide release and/or inhibiting superoxide release in an ischemic organ, and for protecting an organ from damage when isolated from the circulatory system. | 06-07-2012 |
20120149003 | NERVE GRAFT - A nerve graft includes a lyophobic substrate, a carbon nanotube film structure, a protein layer, and a nerve network. The carbon nanotube film structure is located on a surface of the lyophobic substrate. The protein layer is located on a surface of the carbon nanotube film structure away from the lyophobic substrate. The nerve network is positioned on a surface of the protein layer away from the lyophobic substrate. | 06-14-2012 |
20120164622 | Method for Harvesting and Preparing Porcine Hearts for Use in a Cardiac Surgical Simulator - A method is described and disclosed for harvesting and preparing porcine hearts that may be used, among other things, as an improved analog of the human heart in cardiac simulator systems for training surgical personnel for performing beating heart surgery. | 06-28-2012 |
20120178075 | EMULSION AND MOLD-RELEASING AGENT USING THE SAME - Disclosed is an emulsion formed using, as an emulsifier, 0.01 to 30 parts by weight of a perfluoroalkylethyl phosphonic acid salt represented by the general formula: C | 07-12-2012 |
20120190003 | ORGAN ARREST, PROTECTION, PRESERVATION AND RECOVERY - The present invention relates to a composition for controlling viability of a tissue including a potassium channel opener or adenosine receptor agonist, a compound for inducing local anaesthesia and a compound for reducing the uptake of water by a cell in the tissue. The present invention also relates to the use of the composition according to the invention for controlling viability of a tissue. | 07-26-2012 |
20120196270 | METHODS FOR PRESERVING AN ORGAN FOR TRANSPLANTATION USING A HEMOGLOBIN-CARBON MONOXIDE COMPLEX - The present invention relates generally to methods of preserving an organ for transplantation. Specifically, the present invention is directed towards treating an organ with a hemoglobin-carbon monoxide complex to preserve the organ before transplantation. | 08-02-2012 |
20120208172 | Methods of Increasing Liver Proliferation - The present invention is directed to methods of enhancing liver repair after injury, resection or transplantation using antagonists of the bone morphogenetic protein (BMP) signaling pathway in the liver. | 08-16-2012 |
20120214148 | POLYFLUOROALKYLPHOSPHONIC ACID SALT EMULSIFIER AND MOLD-RELEASING AGENT COMPRISING THE SAME AS ACTIVE INGREDIENT - Disclosed is an emulsifier comprising, as an active ingredient, a polyfluoroalkylphosphonic acid salt represented by the general formula: C | 08-23-2012 |
20120244518 | ORGAN COLD STORAGE COMPOSITION AND METHODS OF USE - The present invention provides compositions and methods for decreasing oxidative damage to an organ during cold storage. | 09-27-2012 |
20120258439 | Protein-Free Culture Media Products - Disclosed herein are compositions and methods for a substantially protein-free media (PFM) optimized for stem cell research. | 10-11-2012 |
20120270202 | Pre-Transplant Accommodated Organs Resistant To Anti-Donor Immunity - This invention includes the composition of organ grafts accommodated prior to transplantation and therefore resistant to rejection by preformed antibodies. Accommodation is achieved within the donor animal by administration of sub-lethal levels of accommodation inducing factors derived from animals sensitized to the donor. | 10-25-2012 |
20120270203 | INDOLE AND INDAZOLE DERIVATIVES HAVING A CELL-, TISSUE- AND ORGAN-PRESERVING EFFECT - The present invention relates to a composition for preserving cells, tissues and organs, comprising as an active ingredient indole and indazole compounds of formula (1), or a pharmaceutically acceptable salt or isomer thereof, which are effective for preventing injury of organs, isolated cell systems or tissues caused by cold storage, transplant operation or post-transplantation reperfusion; a preservation method; and a preparation method of the composition. | 10-25-2012 |
20120276518 | Method and Device for Perfusing Tissue by ExVivo Attachment to a Living Organism - The present invention is a holding vessel that has bioreactor and perfusion bioreactor components, a temperature specific environment and holes for transporting substances from a living organism. | 11-01-2012 |
20120288848 | Multi-Dimensional Flow Pad Technology for Covering Three-Dimensional Dome Shaped Anatomies - A three dimensional dome wrap or pad for direct patient surface tissue applications for symmetrical dome shaped anatomy and extremities is disclosed herein. The pad of the present invention comprises a system of smooth streamline mathematically configured channels throughout one or more similarly shaped panels or lobes bonded distinctively to make one unit for the purpose of temperature management of three dimensional objects or subjects when it is applied or inserted. | 11-15-2012 |
20120295245 | DERMAL MICRO-ORGANS, METHODS AND APPRATUSES FOR PRODUCING AND USING THE SAME - Embodiments of the present invention provide Dermal Micro-organs (DMOs), methods and apparatuses for producing the same. Some embodiments of the invention provide a DMO including a plurality of dermal components, which substantially retain the micro-architecture and three dimensional structure of the dermal tissue from which they are derived, having dimensions selected so as to allow passive diffusion of adequate nutrients and gases to cells of the DMO and diffusion of cellular waste out of the cells so as to minimize cellular toxicity and concomitant death due to insufficient nutrition and accumulation of waste in the DMO. Some embodiments of the invention provide methods and apparatuses for harvesting the DMO. An apparatus for harvesting the DMO may include, according to some exemplary embodiments, a support configuration to support a skin-related tissue structure from which the DMO is to be harvested, and a cutting tool able to separate the DMO from the skin-related tissue structure. Other embodiments are described and claimed. | 11-22-2012 |
20120301866 | LIQUID FORMULATION HAVING DISSOLVED GASES USEFUL FOR PRESERVING BIOLOGICAL MATERIAL - The invention relates to a liquid formulation including a liquid solution and at least one gas selected from xenon, argon, hydrogen, H | 11-29-2012 |
20130059285 | AQUEOUS SOLUTION FOR THE PRESERVATION OF TISSUES AND ORGANS - It is provided an improved aqueous solution for the preservation of tissues and organs comprising carvedilol, tacrolimus, and trimetazidine. A synergistic effect is observed for this preservation solution which is particularly effective in marginal organs, such as steatotic livers. | 03-07-2013 |
20130095467 | Method for preserving a whole mammalian organ - Solutions and suspensions comprising polymerized hemoglobin derived from human blood are disclosed. The solutions and suspensions may comprise cell culture medium, an enzyme (such as a protease), and/or a buffer. Processes of preparing the solutions and suspensions are also disclosed. The solutions and suspensions may be employed in methods of isolating mammalian cells, such as pancreatic islets, methods of preserving mammalian tissue and organs, methods of aiding the recovery of mammalian cells following their isolation, methods of maintaining mammalian cells, methods of propagating mammalian cells, and methods of treating a mammal with diabetes. | 04-18-2013 |
20130130222 | DEVICE, KIT AND METHOD FOR USE IN OPHTHALMOLOGY - The present invention refers to a device and a kit for the shipment and the preservation of corneal lenticules ready for use in a lamellar keratoplasty surgery. The present invention also refers to a method for preserving, shipping and prearranging the lenticules so as to facilitate posterior lamellar keratoplasty surgery (or endokeratoplasty). | 05-23-2013 |
20130130223 | NOVEL CONDITIONALLY IMMORTALIZED HUMAN PROXIMAL TUBULE CELL LINE EXPRESSING FUNCTIONAL INFLUX AND EFFLUX TRANSPORTERS - The invention relates to the field of biology and medicine. In particular, the invention relates to the field of pharmaceutical research and drug development, and especially to means for physiological, pharmacological and toxicological research. More in particular, the invention provides renal cell lines with proximal tubular characteristics including multiple influx and efflux transporters. These cell lines are termed ciPTEC. Four representative examples have been deposited at the Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH (DSMZ or German Collection of Microorganisms and Cell Cultures) at Inhoffenstrasse 7 B, 38124 Braun-schweig, Germany under accession number DSM ACC3019, DSM AC-C3020, DSM ACC3021 and DSM ACC3022. | 05-23-2013 |
20130130224 | Method For The Analysis of Biological Samples Through Medical Ultrasound - A method for the conservation of human and animal anatomical parts, both normal and pathological, which comprises a hydration phase, immersion phase and packing phase. The hydration phase comprises immersing the anatomical part in and preserving and hydrating substance. The Immersion and Packing phases comprise immersing the specimen in a gel substance contained in a polyurethane, gel latex or silicone container that allows its analysis and study by ultrasound. | 05-23-2013 |
20130164731 | APPARATUS AND METHODS RELATING TO COLLECTING AND PROCESSING HUMAN BIOLOGICAL MATERIAL CONTAINING ADIPOSE - A portable apparatus for collection and processing of human biological material containing adipose, such as extracted during a lipoplasty procedure, is useful for multi-step processing to prepare a concentrated product (e.g., stromal vascular fraction) or a fat graft composition. The apparatus has a container with a containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a tapered portion to a collection volume for collecting concentrate product. Inlet and suction ports provide access to the tissue retention volume and filtrate volume, respectively, and an extraction port provides versatile access for removal of target processed concentrate material or fat graft material, which access may be via a lumen through a rotatable mixer shaft. Access ports may be configured for access only from above the container. The apparatus is incorporatable into a variety of assemblies, systems, kits, methods and uses. | 06-27-2013 |
20130236879 | THREE-DIMENSIONAL, PREVASCULARIZED, ENGINEERED TISSUE CONSTRUCTS, METHODS OF MAKING AND METHODS OF USING THE TISSUE CONSTRUCTS - Embodiments of the present disclosure provide for three-dimensional (3D), prevascularized, engineered tissue constructs, 3D prevascularized engineered tissue models of cancer, and bioreactors and bioreactor arrays including the tissue constructs. The present disclosure also provides methods of making the tissue constructs, methods of using the tissue constructs, methods of drug discovery using the tissue constructs and/or cancer models, and the like. | 09-12-2013 |
20130302779 | ORGAN PROTECTION, PRESERVATION AND RECOVERY - This application describes compositions, methods of treatment, and methods of manufacturing a medicament for reducing injury or damage to cells, tissues or organs during ischemia, reperfusion, or following ischemia or trauma. The methods for reducing damage to a cell, tissue or organ comprise administering an effective amount of a composition including (i) a potassium channel opener or agonist and/or adenosine receptor agonist; and (ii) an antiarrhythmic agent. The methods may further include postconditioning the cell, tissue or organ. | 11-14-2013 |
20130330706 | Fluid for Suspended Animation - A method to increase the time a donated organ will remain viable prior to transplantation, where the method includes infusing into a human patient declared brain dead, using a first intravenous line, a fluorocarbon fluid comprising a chain length from 1 to about 20 carbon atoms, and optionally synchronously with said infusing, exsanguinating said patient using a second intravenous line. | 12-12-2013 |
20140017657 | ORGAN TRANSPORT APPARATUS WITH SAMPLE COMPARTMENTS - An apparatus for at least one of storage, treatment, assessment and transport of an organ or tissue includes a coolant container configured to cool the organ or tissue, a perfusion circuit configured to perfuse the organ or tissue, and a sample compartment for holding a biological sample. Preferred apparatus has a first internal compartment under a first cover (lid) of the apparatus that includes the coolant container and the sample compartment. The apparatus can include a second internal compartment under a second cover (lid) of the apparatus, the second internal compartment including at least part of the perfusion circuit and a sample compartment. | 01-16-2014 |
20140024010 | METHOD FOR PRODUCING ARTIFICIAL SKIN MODEL, AND ARTIFICIAL SKIN MODEL - Provided is a novel method capable of producing an artificial skin model. A method for producing an artificial skin model includes: providing coated cells ( | 01-23-2014 |
20140051059 | DEHYDRATION DEVICE AND METHODS FOR DRYING BIOLOGICAL MATERIALS - This invention relates generally to a dehydration device and methods for drying biological materials to produce dried biological materials having enhanced structural properties. More specifically, the invention relates to a dehydration device and related methods for drying biological tissue to produce enhanced tissue grafts. | 02-20-2014 |
20140065597 | APPARATUSES FOR AND METHODS OF PROCESSING CELLS AND RELATED STRUCTURES - Apparatus for processing life-based organic particles, including particles selected from the list comprising cells, cellular spheroids, tissues, eukaryotes, micro-organisms, organs or embryos, comprises a hollow volume ( | 03-06-2014 |
20140072951 | FIBER SCAFFOLDS FOR USE IN ESOPHAGEAL PROSTHESES - The development and construction of implantable artificial organs, and a process for manufacturing three-dimensional polymer microscale and nanoscale structures for use as scaffolds in the growth of biological structures such as hollow organs, luminal structures, or other structures within the body are disclosed. | 03-13-2014 |
20140080111 | GRAFT OR TISSUE RINSING SOLUTION AND METHOD FOR RINSING SAID GRAFT OR TISSUE BEFORE REVASCULARIZATION - Extracellular organ or tissue rinsing solution, comprising calcium, PEG with a molecular weight of 35 000 at a concentration of at least 4 g/l, and potassium at a concentration of greater than or equal to 1, but less than 10 mmol/l. | 03-20-2014 |
20140093863 | TRANSPARENT COOLING GEL - For cooling organs and tissues in transplantation medicine, only ice preparations are used so far which are not soft like a gel and are brought to the necessary degree of comminution by mechanically comminuting frozen, sterile, pyrogen-free isotonic infusion solutions, such as 5% glucose or 0.9% saline, with great effort. The lack of gel-like consistency of the preparations and the expensive production process are very disadvantageous. | 04-03-2014 |
20140113273 | PRESERVATION OF BIOMATERIAL PROPERTIES AND METHODS OF STORING - Described herein are enhanced compositions and methods for storing biomaterials. In certain aspects, these biomaterials include natural and engineered eukaryotic tissues. The methods described herein include storing these biomaterials in such a manner that reduces or prevents the loss of biomaterial properties (e.g., extracellular matrix integrity, cell viability, or a combination thereof) occurring either during storage or after removal of the biomaterial from storage. In certain aspects, these biomaterials will be stored in animal product-free solutions containing an agent that prevents or reduces the loss of extracellular matrix integrity. | 04-24-2014 |
20140113274 | ORGAN-PRESERVING COMPOSITION AND USES - The invention relates to the use of a composition comprising at least one globin, one globin protomer or one extracellular hemoglobin of Annelida, a stabilizing solution and/or a solution for conserving organs, said composition having a temperature of between 0° C. and 37° C., for preserving an organ in a donation after brain death donor or a donation after cardiac death donor. | 04-24-2014 |
20140134594 | METHODS FOR CROSS-LINKING BIOPROSTHETIC TISSUE USING BIO-ORTHOGONAL BINDING PAIRS - Methods for treating a bioprosthetic tissue are described. The methods comprise contacting the biological tissue with an anchor compound, the anchor compound comprising first and second functional groups. The first functional group is reactive with and couples a tissue functional group associated with the biological tissue. The second functional group is one of a bio-orthogonal binding pair. The biological tissue coupled to the anchor compound is then exposed to a linking compound. The linking compound comprises at least two functional groups, each comprising the other one of the bio-orthogonal binding pair. In a preferred embodiment, the bio-orthogonal binding pair is an azide and an acetylene. The method can be performed in the presence of a catalyst, preferably a copper catalyst. Alternatively, the method can be performed in the absence of a catalyst, wherein the acetylene is incorporated in a ring-strained cyclic compound, such as cyclooctyne. | 05-15-2014 |
20140147830 | COMPOSITIONS AND METHODS RELATED TO ORGAN OR TISSUE DECELLULARIZATION - Embodiments include methods for decellularizing an organ or tissue comprising conditioning the organ or tissue to be decellularized by exposing the organ or tissue to an electric current, as well as methods of using such decellularized organs or tissues. | 05-29-2014 |
20140147831 | METHODS FOR FORMING NORMAL REGENERATED TISSUES, THE NORMAL REGENERATED TISSUES AND METHODS FOR ASSESSING SENSITIVITIES AND SO ON - A normal regenerated tissue is formed by exposing to radiation a connective tissue or a supporting tissue originating in an organ to thereby form a feeder layer and then transplanting epithelial cells thereon to form a stratified structure. By conveniently and surely providing regenerated tissue by the 3-dimensional culture with the use of a human-origin normal tissue as a base, it is possible to construct systems for assessing effects and side effects of chemicals such as drugs or assessing sensitivities thereof with the use of regenerated tissues as models of corresponding tissues respectively. | 05-29-2014 |
20140154662 | APPARATUS AND PROCESS FOR GROWING A HEART VALVE IN THREE-DIMENSIONS - The present invention relates to a heart valve and, more particularly, to a mold and process shaping and securing cells and tissue layers as they are grown in three-dimensions into a heart valve. | 06-05-2014 |
20140220547 | Methods and Compositions for Vein Harvest and Autografting - The leading cause of graft failure is the subsequent development of intimal hyperplasia, which represents a response to injury that is thought to involve smooth muscle proliferation, migration, phenotypic modulation, and extracellular matrix (ECM) deposition. Surgical techniques typically employed for vein harvest—stretching the vein, placing the vein in low pH, solutions, and the use of toxic surgical skin markers—are shown here to cause injury. The invention therefore provides for non-toxic surgical markers than also protect against stretch-induced loss of functional viability, along with other additives. Devices and compositions for reducing physical stress or protecting from the effects flowing therefrom, also are provided. | 08-07-2014 |
20140234827 | SSAT mRNA TRANSLATION REPRESSION AND ACTIVATION - The invention provides an isolated nucleic acid having a sequence encoding a spermidine/spermine acetyltransferase (“SSAT”), wherein translation of an mRNA comprising the encoded SSAT has increased basal translation and increased stimulated translation, compared to a wild-type mRNA encoding SSAT. Methods of use for the nucleic acid are also provided. Methods and compositions are also provided for reducing ischemia-reperfusion injury in organs or tissue for transplantation. | 08-21-2014 |
20140242569 | Poultry Performance - Methods for increasing breast muscle weight in poultry are disclosed herein. These methods can include delivery of compositions via in ovo administration. The methods disclosed herein also encompass the delivery of proteins. The methods disclosed herein are effective for increasing breast muscle weight in poultry, both in the embryo, and at slaughter age. | 08-28-2014 |
20140272920 | Administration And Monitoring Of Nitric Oxide In Ex Vivo Fluids - Described are systems and methods for monitoring administration of nitric oxide (NO) to ex vivo fluids. Examples of such fluids include blood in extracorporeal membrane oxygenation (ECMO) circuits or perfusion fluids used for preserving ex vivo organs prior to transplanting in a recipient. The systems and methods described herein provide for administering nitric oxide to the fluid, monitoring nitric oxide or a nitric oxide marker in the fluid, and adjusting the nitric oxide administration. | 09-18-2014 |
20140272921 | METHODS AND REAGENTS FOR MAINTAINING THE VIABILITY OF CANCER CELLS IN SURGICALLY REMOVED TISSUE - A composition and method for generating reagents and the composition of these reagents for the stabilization and preservation of viability of cancer tissue which has been surgically excised and the suspension and/or termination of apoptosis (cell death) by significant modulation of cell metabolism by low molar concentrations of synergistic chemistries and hormonal growth enhancers while maintaining normal gene expression patterns of the surgically excised tissue. | 09-18-2014 |
20140272922 | ORGAN/TISSUE DISSECTION BASIN SYSTEM - A basin system is provided comprising a basin, a tissue supporting plate, a platform ring and a suspension rod, the basin system configured to permit a single clinician to effectively and accurately perform the dissection with minimal movement of the resected tissue within the basin system, wherein the platform ring and the suspension rod each comprise a plurality of grooves, permitting a clinician to secure resected tissue within the basin system and tie it down in three dimensions. | 09-18-2014 |
20140302480 | COMPOSITE TISSUE GRAFT AND MATERIALS AND METHODS FOR ITS PRODUCTION AND USE - The technology described herein is directed to tissue engineering, e.g. to biomimetic compositions as well as methods and devices related to producing a biomimetic composition. | 10-09-2014 |
20140329219 | CRITICAL POINT DRYING SYSTEMS AND METHODS FOR IN SITU TISSUE PRESERVATION - Methods and systems for preserving tissues in situ using critical point drying are disclosed. Such methods and systems are particularly applicable to the preservation of a deceased body, such as a deceased person or animal, with or without removal of internal tissues or organs. A fixative can be perfused through the vascular system of the body while blood is removed from the body. The exterior of the body can also be immersed in a bath of fixative. The fixative in the vascular system and the bath can be replaced by subsequent washes of buffer, de-ionized water, and/or alcohol. The alcohol-infused and fixated body can be disposed in a pressure chamber and subjected to a critical point drying process using carbon dioxide. After the critical point drying process, the body is in a preserved state. | 11-06-2014 |
20140342345 | Compositions and Methods for Refolding of Denatured Proteins - Compounds and methods for refolding of proteins in an aqueous solution. In particular, biocompatible multiblock copolymer surfactants such as poloxamers, meroxapols, poloxamines, or polyols are used to catalyze proper refolding without changing the protein composition, and restore the protein to its native conformation and native biological function. The methods can be practiced both in vivo and in vitro. The biocompatible multiblock copolymer surfactants can be used for renaturation of recombinantly expressed proteins, and for renaturation of proteins that are unfolded due to heat, irradiation, mechanical shearing, electrical shock, frostbite, chemical stress, and other abiotic or biotic stresses. | 11-20-2014 |
20140377737 | METHOD FOR CULTURING ISLET CELLS AND METHOD FOR PREPARING CARRIER FOR ISLET CELL TRANSPLANTATION USING ATELOCOLLAGEN, AND ARTIFICIAL PANCREAS PREPARED USING SAME - The present invention provides a method of culturing pancreatic islet cells using a cationized atelocollagen prepared by ionization of high purity atelocollagen, a method of preparing a carrier for pancreatic islet cell transplantation using a cationized atelocollagen, and an artificial pancreas prepared using the same. According to the present invention, it is possible to increase the viability and/or glucose-dependent insulin secretion of pancreatic islet cells during culture of the cells by the use of a cationized atelocollagen or crosslinked atelocollagen scaffold obtained by ionization of high purity atelocollagen. In addition, it is possible to increase the viability and glucose-dependent insulin secretion of cultured and transplanted pancreatic islet cells by the use of a highly stable carrier for pancreatic islet cell transplantation that comprises cationized atelocollagen and alginate. | 12-25-2014 |
20150044663 | TECHNIQUE FOR REGULATING REGENERATION OF TISSUE OR FAULTY OR ABNORMAL PART IN ORGAN USING NELL-1 - The object aims to form and maintain a cell, a tissue or an organ induced by differentiation. Disclosed is a composition for inducing the differentiation of a cell capable of being differentiated in a given direction to thereby produce a cell, a tissue or an organ through the further induction of the differentiation in the given direction. The composition comprises NELL-1 or a substance which can be altered so as to act as NELL-1 upon the differentiation. Also disclosed is a composition for maintaining a cell, a tissue or an organ produced by the induction of the differentiation. | 02-12-2015 |
20150064686 | OXYGEN UPTAKE COMPOSITIONS AND PRESERVATION OF OXYGEN PERISHABLE GOODS - The preservation or extension of the life of oxygen perishable products such as fresh or cooked meats; fresh vegetables and fruits; dried vegetables, fruits and meats; packaged pharmaceuticals or biologicals; open and enclosed marketing display cases of meats, fruits and vegetables; and packaging, warehousing and transportation of meats, fruits, and vegetables is obtained by subjecting such products to an atmosphere obtained from gas permeable packets of an oxygen scavenging composition comprising a sodium carbonate mineral and one or more carboxylic acids. When the environment surrounding the oxygen perishable product is subjected to the packets of gaseous permeable oxygen scavenging compositions, oxygen is taken in by the scavenging composition and also the presence of carbon dioxide in the surrounding environment is enhanced thereby minimizing or eliminating the effect the oxygen on the oxygen perishable products. | 03-05-2015 |
20150104781 | ORGAN STORAGE SOLUTION - The invention relates to a storage solution for the preservation of organs, tissue or organ systems to be transplanted, said solution being based on the known HTK, UW or Celsior solutions. In accordance with the invention it has been determined that ischemia and reperfusion injury could be reduced through the addition of ectoine or hydroxyectoine. | 04-16-2015 |
20150111193 | METHODS AND SOLUTIONS FOR TISSUE PRESERVATION - Described herein are compositions and methods particularly useful in the medical arts. The compositions and methods may be used in connection with the preservation of a portion of a mammal, for example, tissues, organs, appendages, limbs, extremities, stem cells, myocytes, bone marrow, skeletal muscle as well as an array of other medical procedures, such as cardiac surgery, transplantation and/or preservation. In various embodiments, the inventive composition may be hyperoxygenated and be formulated to resemble the biochemistries of natural intracellular fluids. The inventive composition includes active ingredients to reduce ischemic, hypothermic and reperfusion injury during transplantation, thereby resulting in improved post-transplant graft function and quality, when used in connection with organ transplantation and storage procedures, for example cardiac transplantation. | 04-23-2015 |
20150118673 | POLYMERIC SORBENT FOR REMOVAL OF IMPURITIES FROM WHOLE BLOOD AND BLOOD PRODUCTS - The invention concerns methods of treating stored blood and blood products maximizing shelf life and/or minimizing transfusion related complications such as non-hemolytic transfusion reactions such as fever, transfusion-related acute lung injury (TRALI), transfusion associated dyspnea (TAD), and allergic reactions by removing undesirable molecules in the blood and blood products milieu through use of a sorbent. | 04-30-2015 |
20150140543 | COMPOSITIONS AND METHODS FOR ENHANCING THE VIABILITY OF ANIMAL CELLS, TISSUES, AND ORGAN EXPLANTS - Disclosed are compositions and methods for the preservation, storage, and transport of living biological tissues, organs, and populations of isolated cells. In particular, the disclosed compositions and processes permit mammalian cells, tissues, and organs to be harvested from suitable donor animals, stored for prolonged periods, and transported to the site of recipient implantation, all without significant loss of cell viability, biological activity, and/or tissue integrity. | 05-21-2015 |
20150313209 | SOLUTION FOR PRESERVING VASCULAR CONDUITS - Organ and tissue preservation formulations with improved stability shelf life are provided by separating the formulation into a first solution having a pH of at least 7 and a second solution having a pH of less than 7. The first solution includes components with improved stability when stored at a pH of 7.0 or above, and the second solution includes components with improved stability when stored at a pH below 7.0. The first solution includes water, a balanced salt solution, a sugar, and L-arginine at a pH of at least 7.0. The second solution includes water, ascorbic acid, and reduced glutathione at a pH of less than 7.0 and preferably from pH 6.9 to about pH 2.8. During use, the first and second solutions are mixed to form a final formulation that can be used at a physiological pH to preserve the function of the tissue or organ. | 11-05-2015 |
20150327539 | METHODS AND REAGENTS FOR MAINTAINING THE VIABILITY OF CANCER CELLS IN SURGICALLY REMOVED TISSUE - A composition and method for generating reagents and the composition of these reagents for the stabilization and preservation of viability of cancer tissue which has been surgically excised and the suspension and/or termination of apoptosis (cell death) by significant modulation of cell metabolism by low molar concentrations of synergistic chemistries and hormonal growth enhancers while maintaining normal gene expression patterns of the surgically excised tissue. | 11-19-2015 |
20150342175 | ORGAN AND TISSUE PRESERVATION FORMULATIONS WITH INCREASED STABILITY AND SHELF LIFE - Organ and tissue preservation formulations with improved stability shelf life are provided by separating the formulation into a first solution having a pH of at least 7 and a second solution having a pH of less than 7. The first solution includes components with improved stability when stored at a pH of 7 or above, and the second solution includes components with improved stability when stored at a pH below 7. The first solution includes water, a balanced salt solution, a sugar, adenosine, orotic acid, malic acid, L-carnitine, and insulin. The second solution includes water, an antioxidant such as ascorbic acid, a cellular reducing agent such as reduced glutathione, L-citrulline, creatine monohydrate, L-carnosine, and L-arginine. During use, the first and second solutions are mixed to form a formulation final formulation that can be used at a physiological pH to preserve the function of the tissue or organ. | 12-03-2015 |
20150351380 | TISSUE-PRESERVING LIQUID AND TISSUE-PRESERVING METHOD - The present invention provides a tissue preservation solution that has good quality for preservation of tissues, organs and cells, and is useful in the fields of regenerative medicine, transplantation medicine, etc. The tissue preservation solution of the present invention comprises a compound having a Nrf2 activating effect. Preferable compounds having a Nrf2 activating effect are electrophiles, and in particular, ebselen and tert-butylhydroquinone. A preferable basal solution of the tissue preservation solution is Hank's balanced salt solution. | 12-10-2015 |
20160002602 | Bioengineered Liver Constructs And Methods Relating Thereto - An in vitro liver organoid is provided along with methods of making and using the organoid. The liver organoid can be manipulated to have either immature or mature characteristics. Some manipulations generate a liver organoid that supports expansion and/or differentiation of hematopoietic stem cells. | 01-07-2016 |
20160081327 | ORGAN AND TISSUE PRESERVATION SOLUTIONS HAVING INCREASED OXYGEN-CONTENT, STABILITY AND SHELF LIFE - Organ and tissue preservation solutions having improved formulations are comprised of two separate solutions. The first solution includes one or more salts, water, dissolved oxygen, lactobionic acid, mannitol, glutamic acid and histidine at a pH of at least 7, preferably from about 7.3 to about 8.3. The second solution includes water and reduced glutathione at a pH of below 7.0, preferably from about 3 to 6 wherein oxygen present in the solution is removed. The two formulations are mixed together at the point of use resulting in an organ and tissue preservation solution having improved stability and that contains oxygen to prevent ischemia in the preserved organs. The present invention also includes kits that contain the two formulations. | 03-24-2016 |
20160088832 | FORMULATIONS CONTAINING POLY (0-2 HYDROXYETHYL) STARCH FOR INCREASING THE OXYGEN-CONTENT, STABILITY AND SHELF LIFE OF AN ORGAN AND TISSUE PRESERVATION SOLUTION - Organ and tissue preservation solutions having improved formulations. The improved solutions are comprised of two separate solutions. The first solution, is comprised of one or more salts, water, dissolved oxygen, Poly (0-2-hydroxyethyl) starch, lactobionic acid, adenosine, raffinose and allopurinol and said first solution has a pH of at least 7.0;, preferably from about 7.3 to about 8.2; and a second solution comprised of water, and reduce glutathione at a pH of below 7.0, preferably from about 3 to 6 wherein oxygen present in the solution is removed. The two formulations are then mixed together at the point of use resulting in the organ and tissue preservation solution having improved stability and that contains oxygen to prevent ischemia in the preserved organs. The present invention is also comprised of kits that contain the two formulations. | 03-31-2016 |
20160088833 | TISSUE PRESERVATION SYSTEM - The present invention provides a method and apparatus for tissue, such as an allograft, storage and preservation for extended periods of time at room temperature in a sterile tissue culture chamber. The invention further provides a process for maintaining the sterility of tissue using the apparatus as described. | 03-31-2016 |
20160095308 | SYSTEMS AND METHODS FOR EX-VIVO ORGAN CARE - The invention provides, in various embodiments, systems, devices and methods relating to ex-vivo organ care. In certain embodiments, the invention relates to maintaining an organ ex-vivo at near-physiologic conditions. | 04-07-2016 |
20160120171 | ALPHA CONNEXIN C-TERMINAL (ACT) PEPTIDES FOR USE IN TRANSPLANT - The present disclosure provides compositions and methods for treating or preventing macular degeneration in a subject. The disclosure also provides compositions and methods for preserving organs and tissues for transplantation, and for preventing cellular injury in organs or in subjects. | 05-05-2016 |
20160144147 | ARRANGEMENT AND METHOD FOR THE IN-VITRO and IN-VIVO TREATMENT OF BRONCHIAL TUMORS - The invention relates to an arrangement and method for the in-vitro and in-vivo treatment of bronchial tumors. | 05-26-2016 |
20160158415 | METHOD FOR DETOXIFYING A BIOLOGICAL TISSUE - Biological tissues may be prepared for use in biological prostheses. The biological tissue may be fixated with glutaraldehyde and may be subjected to successive treatment of the tissue with a solution containing taurine to neutralize excess aldehyde groups that remain free after fixation. | 06-09-2016 |
20160192639 | Method and Composition for Hypothermic Storage of Placental Tissue | 07-07-2016 |
20160249603 | SOLUTIONS FOR INCREASING THE STABILITY AND SHELF LIFE OF AN ORGAN AND TISSUE PRESERVATION SOLUTION | 09-01-2016 |