WATERS TECHNOLOGIES CORPORATION Patent applications |
Patent application number | Title | Published |
20160139136 | RAPID FLUORESCENCE TAGGING OF GLYCANS AND OTHER BIOMOLECULES WITH ENHANCED MS SIGNALS - Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described. | 05-19-2016 |
20160136544 | METHODOLOGY FOR SCALING METHODS BETWEEN SUPERCRITICAL FLUID CHROMATOGRAPHY SYSTEMS - A methodology scales supercritical fluid chromatography (SFC) and/or carbon dioxide based chromatography methods between different system and/or column configurations. The methodology includes measuring an average mobile phase density during a first separation utilizing CO2 as a mo bile phase component and substantially duplicating the average density profile for a second separation. Substantial duplication of the average mobile phase density (e.g., within about 10%, 5%, 2.5%, 1%, 0.5%, 0.1 %, 0.05%) results in chromatography for both system and/or column configurations having similar selectivity and retention factors. Average mobile phase density may be, either measured directly, calculated, or approximated using average pressure or density measurements. The average pressure profile may be used as a close approximation to duplicate average density profiles between separations. | 05-19-2016 |
20160131620 | ENHANCED SENSITIVITY OF DETECTION IN ELECTROSPRAY IONIZATION MASS SPECTROMETRY USING A POST-COLUMN MODIFIER AND A MICROFLUIDIC DEVICE - A microfluidic liquid chromatography-electrospray ionization (LC-ESI) device is provided for enhancing the sensitivity of mass spectrometric detection of an analyte in a sample. The device is designed to drive effective intermixing of an analytical flow stream exiting a chromatographic stationary phase and a post-column modifier reagent. The mixed flow stream thus obtained is used for generating an electrospray containing analyte ions. Also provided are methods for enhanced sensitivity of detection of an analyte in a sample. | 05-12-2016 |
20160114262 | CHROMATOGRAPHIC FILTER - The invention provides filters and methods of assembling filters. In an exemplary embodiment, the filter includes a porous element, a compression element, and a housing. The compression element can be configured to receive the porous element, thereby forming an assembly. For example, the compression element can receive the porous element in a slip-fit relationship. The housing can have an opening formed therein that is configured to receive the assembly. In some embodiments, the assembly can be retained within the opening when the assembly is received therein. For example, the opening can receive the assembly in a press-fit relationship. | 04-28-2016 |
20160077061 | SAMPLING SYSTEMS AND METHODS OF USING THE SAME - A sampling system comprising an external sampling assembly in fluidic communication with a process sample manager is provided herein. The sampling system can automatically acquire sample from one or more sources of sample to prepare sample for injection into a column or detector. The external sampling assembly has an external sampling valve connected to an external pump and is in fluidic communication with a process sample manager. The external sampling valve has a first configuration and a second configuration useful in three steps of drawing, loading and discharging sample. Two selection valves can be connected to a plurality of external sampling valves for sequential sampling of multiple sources of sample. Sample can also be diluted in the process sample manager and then combined in a solvent composition stream for injection into the column or the detector. | 03-17-2016 |
20160077060 | PROCESS SAMPLE AND DILUTION SYSTEMS AND METHODS OF USING THE SAME - Process sample and dilution systems automatically draw sample from a reactor or other source of sample and prepare drawn sample for subsequent injection to a chromatographic column or a detector. The process sample and dilution system has an external pump valve assembly in fluidic communication with a process sample manager. The external pump valve assembly includes an external sampling valve, an external process valve, external pump and a mixing tee. The external sampling valve can be configured to automatically acquire sample from a reactor or other source of sample. The external pump discharges wash from the external process valve whereby sample is discharged from the external sampling valve to the mixing tee for quenching and dilution and subsequent processing by the process sample manager. | 03-17-2016 |
20160054275 | METHOD FOR EXTENDING THE DYNAMIC RANGE OF ABSORBANCE DETECTORS - The invention generally provides methods for improving the dynamic range of an absorbance detector and absorbance detectors having improved dynamic range. In an exemplary embodiment, the method includes receiving calibration data for a plurality of samples, the calibration data comprising an absorbance for a concentration of each of the samples, calculating a contribution of stray light to the calibration data, and correcting subsequent data by removing the contribution of stray light. | 02-25-2016 |
20160038853 | APPARATUS AND METHODS FOR CONTROLLING THE TEMPERATURE OF A CHROMATOGRAPHY COLUMN - An apparatus for controlling the temperature of a chromatography column includes a thermal-isolation vessel; a heater in thermal communication with the chromatography column and disposed on the thermal-isolation vessel; a temperature sensor disposed to directly measure the temperature of the chromatography column; and a control unit in signal communication with the temperature sensor to control the heater in response to LIGHT the direct measurement. | 02-11-2016 |
20160033599 | Devices for Use in Solid-State NMR Analysis - The present disclosure relates to devices and methods for use in SSNMR analysis of solid particulate samples. These devices and methods are configured to contain a solid particulate sample as it undergoes SSNMR analysis while also operating to attenuate peak broadening in the resulting spectrum due to anisotropic dipole coupling interactions and CSA during such analysis by generating a substantially fluidized bed of the solid particulate sample. | 02-04-2016 |
20150367326 | POROUS INORGANIC/ORGANIC HYBRID PARTICLES HAVING HIGH ORGANIC CONTENT AND ENHANCED PORE GEOMETRY FOR CHROMATOGRAPHIC SEPARATIONS - Novel particles and materials for chromatographic separations, processes for preparation and separations devices containing the chromatographic particles and materials are provided by the instant invention. In particular, the invention provides a porous inorganic/organic hybrid particle, wherein the inorganic portion of the hybrid particle is present in an amount ranging from about 0 molar % to not more than about 49 molar %, wherein the pores of the particle are substantially disordered. The invention also provides a porous inorganic/organic hybrid particle, wherein the inorganic portion of the hybrid particle is present in an amount ranging from about 25 molar % to not more than about 50 molar %, wherein the pores of the particle are substantially disordered and wherein the particle has a chromatographically enhancing pore geometry (CEPG). Methods for producing the hybrid particles, separations devices comprising the hybrid particles and kits are also provided. | 12-24-2015 |
20150338384 | IONIZATION OF ANALYTE MOLECULES COMPRISED IN A FLOW OF GAS - An apparatus for ionizing analyte molecules comprised in a flow of a first gas. The apparatus includes an inlet tube through which the first gas may be discharged into an ionization region. The apparatus also includes a nozzle electrode disposed around the inlet tube to define a substantially annular space between the exterior of the inlet tube and the interior of the nozzle electrode. The sheath tube includes an inlet for introducing a fluid into the substantially annular space and an outlet through which the fluid may be discharged into the ionization region. The apparatus is configured to ionize the analyte molecules optionally via electrospray or chemical ionization. | 11-26-2015 |
20150330956 | Flame Ionization Detection Burner Assemblies for Use in Compressible Fluid-Based Chromatography Systems - The present disclosure relates to burner assemblies of flame-based detectors. These burner assemblies are configured to deliver decompressed mobile phase of supercritical fluid chromatography systems to the flame of a flame-based detector while providing for improved optimization of analyte response as well as enhanced flame stability during operation. | 11-19-2015 |
20150301000 | FLAME IONIZATION DETECTION FOR SUPERCRITICAL FLUID CHROMATOGRAPHY EMPLOYING A MATCHED SEPARATION COLUMN AND FLAME BURNER - The present disclosure relates to flame based detection methods for compressed mobile phase chromatography. In particular, the present disclosure relates to the operation of a flame ionization detector for carbon dioxide based chromatography, such as supercritical fluid chromatography. The present disclosure includes a method of matching a chromatographic column with a flame ionization detector inner burner including providing a chromatographic column with an internal diameter, determining an optimal mobile phase flow rate for the chromatographic column, calculating an optimal inner diameter of the inner burner that combined with the internal diameter and flow rate of the column produces optimal detector performance, and providing a flame ionization detector inner burner having an inner diameter substantially equal to the calculated optimal inner diameter. | 10-22-2015 |
20150276691 | CHROMATOGRAPHY COLUMN - A chromatographic separation device includes a chromatographic separation module and a memory component. The memory component is attached to the chromatographic separation module, contains information related to the chromatographic separation module with respect to the entire lifetime thereof, and includes a write-once-read-many (WORM) area where the information is written. | 10-01-2015 |
20150276690 | RAPID ANALYSIS OF STEROIDS AND STEROID DERIVATIVES - The subject technology is directed to a CO | 10-01-2015 |
20150253206 | QUASIADIABATIC DIFFERENTIAL SCANNING CALORIMETER - A method of operating a differential scanning calorimeter wherein errors in the heat flow rate measurement are reduced by operating the calorimeter in a quasiadiabatic mode and by employing a heat flow rate measurement algorithm that includes the leakage heat flow rate. The temperature of the DSC enclosure is controlled independently of the temperature of the measuring system, which allows the temperature difference between the sample and reference containers and the enclosure to be minimized, thus minimizing leakage heat flow. | 09-10-2015 |
20150212056 | SEPARATION EFFICIENCY IN SUPERCRITICAL FLUID CHROMATOGRAPHY - The present technology uses one or more separating segments, i.e. chromatography columns, aligned in series along a flow path. The separating segments are divided by a plurality of heating elements or are heated directly. The heating elements heat the supercritical mobile phase and sample to replace heat lost due to axial expansion of the mobile phase along the mobile phase flow path. | 07-30-2015 |
20150204826 | APPARATUS AND METHODS FOR PREPARATIVE LIQUID CHROMATOGRAPHY - Embodiments of the present invention feature methods and apparatus for performing chromatographic separations. The invention feature methods and control means in the form of computer programs and software that control the formation of a concentration gradient at two or more total flow rates, with an elution period occurring in a slow flow rate and at least other portions of the gradient occurring during a fast total flow rate. | 07-23-2015 |
20150184760 | INJECTION-COMPRESSION MOLDED ROTORS - A shear valve assembly for use in a high performance liquid chromatography system. The shear valve assembly includes a stator having a plurality of first fluid-conveying features; and a rotor having one or more second fluid-conveying features. The rotor is movable, relative to the stator, between a plurality of discrete positions such that, in each of the discrete positions, at least one of the one or more second fluid-conveying features overlaps with multiple ones of the first fluid conveying features to provide for fluid communication therebetween. The rotor, including the second fluid-conveying features, is formed by injection-compression molding. | 07-02-2015 |
20150179421 | ION OPTICAL ELEMENT - An ion optical element that may be used as an ion guide in a mass spectrometer, as a reflectron in a time-of-flight mass spectrometer, as an ion mobility drift tube in an ion mobility spectrometer, or as a collision cell or reaction cell in a mass spectrometer. The ion optical element has an inner tube made of a first ceramic material within an outer ceramic tube made of a second ceramic material. The electrical resistivity of the second ceramic material is two orders of magnitude or more higher than the electrical resistivity of the first ceramic material. In certain embodiments, the thermal conductivity of the second ceramic material is at least about an order of magnitude higher than the thermal conductivity of the first ceramic material. | 06-25-2015 |
20150144782 | SYSTEMS, DEVICES, AND METHODS FOR CONNECTING A CHROMATOGRAPHY SYSTEM TO A MASS SPECTROMETER - The invention provides interfaces between analytical instruments, e.g., between chromatography systems and mass spectrometers. In an exemplary embodiment, an ion source is provided for connecting a carbon dioxide-based chromatograph device to a mass spectrometer. The ion source includes a first conduit for receiving eluent from the chromatography device, a heater for heating at least a portion of said first conduit, a second conduit in fluid communication with the first conduit, an inlet for receiving eluent from said second conduit and introducing the eluent into an ion source region to form a plume of gas and/or liquid in the ion source region, and an ionization promoting inlet for injecting an ionization promoting fluid into the ion source region to interact with the plume to promote ionization of at least some of the plume. | 05-28-2015 |
20150142367 | TECHNIQUES FOR USE WITH TEST QUALIFICATION PROTOCOLS - Described are techniques for performing qualification protocol testing. A qualification entity category is selected indicating a category of an entity for which a qualification test protocol is generated. One or more qualification tests are selected and included in the qualification test protocol. Test information is specified for the one or more qualification tests. The test information includes first information identifying one or more code modules executed to perform said one or more qualification tests, second information identifying test inputs to said one or more code modules, and third information specifying how to evaluate tests results from executing each of said one or more qualification tests. A qualification protocol structure including said test information is generated. | 05-21-2015 |
20150136700 | CHROMATOGRAPHIC MATERIALS - The present invention provides a chromatographic stationary phase material various different types of chromatography. One example chromatographic stationary phase is represented by Formula 1 [X](W) | 05-21-2015 |
20150135861 | PRE-COLUMN HEATING OF MOBILE PHASE SOLVENT IN CHROMATOGRAPHY SYSTEMS - An apparatus for heating a flowing fluid includes a tubing assembly, a heater block made of thermally conductive material, and a heater cartridge in thermal communication with the heater block. The heater cartridge is configured to provide heat to the heater block for transfer to fluid flowing through the tubing assembly. The apparatus also includes circuitry in electrical communication with the heater cartridge to control a temperature of the heater block by controlling operation of the heater cartridge. The heater block is die-cast about the tubing assembly. | 05-21-2015 |
20150133294 | CHROMATOGRAPHIC MATERIALS - In one aspect, the present invention provides a chromatographic stationary phase material for various different modes of chromatography represented by Formula 1: [X](W) | 05-14-2015 |
20150129494 | METHOD AND AN APPARATUS FOR CONTROLLING FLUID FLOWING THROUGH A CHROMATOGRAPHIC SYSTEM - A method for controlling fluid flowing through a chromatographic system includes determining a fluidic parameter related to density at a first fluidic location in the chromatographic system; and in response to the determined fluidic parameter, modifying a volumetric flow rate or a pressure at a second fluidic location in the chromatographic system to produce a selected mass flow rate of the fluid. | 05-14-2015 |
20150129474 | TECHNIQUES FOR THERMALLY INSULATING A LIQUID CHROMATOGRAPHIC COLUMN - An apparatus for performing liquid chromatography includes a chromatography column, and an insulating member surrounding the chromatography column wherein the insulating member is formed from a vacuum chamber surrounding the chromatography column. Another apparatus for performing liquid chromatography includes a chromatography column, and an insulating member surrounding the chromatography column, wherein the insulating member includes aerogel. Also described is a method of insulating a chromatography column comprising forming a jacket surrounding the chromatography column, and creating a vacuum chamber in an area between the jacket and the chromatography column. | 05-14-2015 |
20150129057 | STATIC BACK PRESSURE REGULATOR - The invention generally provides a static back pressure regulator. In exemplary embodiments, the static back pressure regulator includes a seat that defines part of a fluid pathway, a poppet, a spring arranged to bias the poppet toward the seat to restrict fluid flow through the fluid pathway, and a calibration element configured to adjust a force applied to the poppet by the spring. The calibration element can include a through hole that forms part of the fluid pathway. The poppet can include a first guiding portion that extends into the through hole of the calibration element and inhibits tipping of the poppet relative to the seat. | 05-14-2015 |
20150122988 | CHROMATOGRAPHY APPARATUS AND METHODS USING MULTIPLE MICROFLUIDIC SUBSTRATES - An apparatus for chemical separations includes a first substantially rigid microfluidic substrate defining a first fluidic port; a second substantially rigid microfluidic substrate defining second fluidic port; and a coupler disposed between the first and second substrates, the coupler defining a fluidic path in fluidic alignment with the ports of the first and second substrates. The coupler includes a material that is deformable relative to a material of the first substrate and a material of the second substrate. The substrates are clamped together to compress the coupler between the substrates and form a fluid-tight seal. | 05-07-2015 |
20150118762 | METHOD AND AN APPARATUS FOR IONIZING A SAMPLE - A method of ionizing a sample includes providing an aqueous liquid and directing a jet comprising carbon dioxide to interact with the provided aqueous liquid. One or both of the aqueous liquid and the jet comprise the sample. At least a portion of the sample is ionized due to the interaction. | 04-30-2015 |
20150101419 | Phase Detection in Multi-Phase Fluids - The present disclosure relates to phase detection in multi-phase fluids where two fluid phases can be present in the fluid. Phase detection apparatus and methods are disclosed for determining the phase(s) (e.g., supercritical, liquid, and/or gas) of a fluid in a multi-phase fluid system, such as carbon dioxide based separation and chromatography system. | 04-16-2015 |
20150101393 | MODULATED FLAME GAS FLOW RATES IN FLAME-BASED DETECTORS - Methods and apparatus for the modulation of flame gas stoichiometry to a flame-based detector for use in chromatographic separations are presented. As the total mass flow rate of mobile phase entering the flame-based detector changes (e.g., as a result of density programming in the separation), the mass flow rate of combustion gases to the detector are altered in proportion to the amount of mobile phase entering the detector. As a result, flame stability and sensitivity of the detector can be maintained by the methods and apparatus of the present disclosure. | 04-16-2015 |
20150083947 | BACK PRESSURE REGULATION - The invention generally provides a dynamic back pressure regulator. In an exemplary embodiment, the back pressure regulator includes an inlet, an outlet, a seat disposed between the inlet and the outlet and defining at least part of a fluid pathway, and a needle displaceable relative to the seat to form a restriction region therebetween for restricting fluid flow between the inlet and the outlet. In some embodiments, the needle can include a corrosion and/or erosion resistant polymer tip. | 03-26-2015 |
20150079691 | CATALYTIC OXIDATION OF POLAR MODIFIERS IN CHROMATOGRAPHIC MOBILE PHASES - The present disclosure relates to an oxidizer, and related methods, for oxidizing polar modifiers in chromatographic mobile phases. The oxidizer enables the use of flame-based detection in chromatographic separations, such as carbon dioxide based chromatography, which employ polar modifiers, such as methanol. Upon exiting a chromatographic column, the mobile phase containing the polar modifier is flowed through an oxidizer that contains a catalyst to oxidize at least a portion of the polar modifier to a species that does not interfere with the function of the flame-based detector. The oxidizer allows for flame-based detection, such as flame ionization detection, in applications in which a polar modifier with a reduced form of carbon is used. | 03-19-2015 |
20150078962 | MICROFLUIDIC FLAME IONIZATION DETECTOR - The present disclosure relates to a microfluidic flame ionization detector for use in small scale separations, such as, for example, microfluidic gas chromatography and microfluidic carbon dioxide based fluid chromatography. In some arrangements, the microfluidic counter-current flame ionization detector employs a non-parallel arrangement for the introduction of combustion gases into the combustion chamber. In other arrangements, the detector housing is configured to incorporate at least one of the detector electrodes within the housing using electrically isolating fittings. | 03-19-2015 |
20150059864 | METHOD, SYSTEM AND APPARATUS FOR AUTOMATIC CALIBRATION OF A NEEDLE VALVE DEVICE IN A PRESSURIZED FLOW SYSTEM - Exemplary embodiments are directed to apparatus, systems and methods used in connection with a needle valve device operating in a pressurized flow system. The apparatus, systems and methods provide for automatic positioning of a needle relative to a seat in the needle valve device to provide consistent calibration with minimal user interaction after a maintenance event or upon a start-up of the pressurized flow system. The apparatus, systems and methods utilize a calibration collar secured to a shaft of an actuator within the needle valve device. The calibration collar includes one or more locking mechanism and a spring. | 03-05-2015 |
20150047730 | METHOD AND APPARATUS FOR SAMPLE INJECTION IN LIQUID CHROMATOGRAPHY - The present invention provides a method and apparatus for substantially eliminating destructive transients of pressure or flow rate which can degrade the efficiency and useful lifetime of chromatography columns. The present invention enables a substantially constant flow of mobile phase liquid to be maintained through the chromatography system by eliminating the flow blockage interval associated with the actuation of sample injection valves. The present invention further provides a method to reduce the pressure and flow rate transients associated with pressurization of the sample loop contents when the sample loop is introduced to chromatography system delivery pressure. | 02-19-2015 |
20150027567 | BACK PRESSURE REGULATION - The invention generally provides a dynamic back pressure regulator. In exemplary embodiments, the back pressure regulator includes an inlet, an outlet, a seat disposed between the inlet and the outlet and defining at least part of a fluid pathway, and a needle displaceable relative to the seat to form a restriction region therebetween for restricting fluid flow between the inlet and the outlet. In some embodiments, the needle can be formed of a chemically resistant ceramic or have a metal plating to provide corrosion and/or erosion resistance. | 01-29-2015 |
20150024510 | PERFORMING CHEMICAL REACTIONS AND/OR IONIZATION DURING GAS CHROMATOGRAPHY-MASS SPECTROMETRY RUNS - A gas chromatography-mass spectrometry (GC-MS) method that includes performing a first GC-MS run on a sample using a gas chromatography-mass spectrometry system. Performing the first GC-MS nm includes i) passing a first flow of a carrier gas carrying a first portion of the sample through a gas chromatograph to provide a first effluent; ii) generating first ions under protonation conditions by passing the first effluent through an atmospheric pressure ionization source; iii) passing the first ions through a mass spectrometer; and iv) recording first GC-MS data for the first ions. The method also include performing a second GC-MS run on the sample using the gas chromatography-mass spectrometry system. | 01-22-2015 |
20150021265 | FLOW SPLITTING IN SUPERCRITICAL FLUID CHROMATOGRAPHY SYSTEMS - A method includes passing a mobile phase fluid flow comprising liquefied CO2 through a separation column; then introducing a makeup fluid flow into the mobile phase fluid flow to form a mixed fluid flow; and then splitting the mixed fluid flow. | 01-22-2015 |
20150014563 | SYSTEM AND METHOD FOR MINIMIZATION OF FORCE VARIATION IN A SOLENOID WITHIN A PRESSURIZED FLOW SYSTEM - Exemplary embodiments are directed to systems and methods used to minimize force variation from a solenoid through an operating stroke. The systems and methods generate a near constant or substantially constant force solenoid assembly which can be used in a force driven device, such as, for example, a pressure regulator for accurately controlling pressure in a pressurized flow system. The systems and methods are based on an initial solenoid characterization and include an application of springs and placement of the solenoid within the system at a gap distance to minimize variation in force in the operating stroke of a commercially available solenoid. | 01-15-2015 |
20140370613 | Atmospheric Pressure Chemical Ionization Detection - An atmospheric pressure chemical ionization detector includes a reaction chamber that is configured to receive gas phase analytes. An electrode is disposed within the reaction chamber and is configured to ionize the gas phase analytes via corona discharge. A collector is disposed adjacent an outlet of the reaction chamber and is configured to attract ions from the chamber such that the ions hit the collector to induce a measurable current. The detector is configured for non-mass spectrometric detection of gas phase analyte ions. | 12-18-2014 |
20140367353 | DEVICES FOR CONTAINING MATERIALS AND METHODS OF USING AND MARKING SAME - Embodiments of the present invention feature devices and methods for containing one or more compounds. The inventions feature devices having an ampoule-like closure to preserve the integrity of materials contained therein with vial-like features to allow closure after opening. The devices are sized to be received in a tray or carousal of an autosampler. | 12-18-2014 |
20140350263 | RAPID FLUORESCENCE TAGGING OF GLYCANS AND OTHER BIOMOLECULES WITH ENHANCED MS SIGNALS - Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described. | 11-27-2014 |
20140345372 | MANAGING FLUIDIC CONNECTIONS TO MICROFLUIDIC DEVICES - A system (e.g., a chromatography system) includes a rotor, a microfluidic device, a rotor driver, a clamping mechanism, and control electronics. The rotor defines a plurality of first fluid-conveying features. The micro fluidic device defines one or more channels and a plurality of second fluid-conveying features, in fluid communication with the one or more channels. The rotor driver is coupled to the rotor and is configured to rotate the rotor, relative to the microfluidic device, between a first position and a second position such that, in each of the positions, at least one of the first fluid-conveying features cooperates with at least one of the one or more channels to provide for fluid communication therebetween. The clamping mechanism is operable to provide a sealing force to establish a fluid-tight seal between the rotor and the microfluidic device. | 11-27-2014 |
20140338432 | METHODS OF SEPARATING LIPIDS - The invention provides novel, simple, reliable and effective methods for separating various lipids from biological samples. In certain embodiments, the invention provides HILIC-based UHPLC separation methods. In one embodiment, the methods of the invention offer an efficient and rapid separation of ether glycophospholipids (e.g., plasmalogens) from their diacyl or monoacyl counterparts within the same lipid class. In certain instances, the invention relates to the use of a Waters ACQUITY UPLC® system. | 11-20-2014 |
20140338429 | METHOD OF SEPARATION OF LIPID AND BIOLOGICAL MOLECULAR SPECIES USING HIGH PURITY CHROMATOGRAPHIC MATERIALS COMPRISING AN IONIZABLE MODIFIER - The present invention provides methods of separating, profiling and analyzing molecular species contained within a biological sample using a high purity chromatographic material (HPCM) comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifiers with the proviso that when the ionizable modifier does not contain a Zwitterion, the ionizable modifier does not contain a quaternary ammonium ion moiety. | 11-20-2014 |
20140330524 | TECHNIQUES FOR QUANTIFICATION OF SAMPLES - Techniques are described for quantification of molecules in a sample. Mass spectrometry is performed to obtain ionization intensities for precursor and product ions originating from a particular molecule. A first stet of precursor ions having the highest ionization intensities and originating from the particular molecule is determined. For each of the one or precursors in the first set, determined is a second set of one or more product ions that are fragments associated with said each precursor and have the highest ionization in intensities of product ions associated with said each precursor. An intensity sum is calculated for the particular molecule by adding ionization intensities of product ions included in the second sets for the one or more precursors in the first set. The intensity sum is compared to information included in a calibration standard. A quantity of the particular molecule in the sample is determined based on said comparing. | 11-06-2014 |
20140329919 | POROUS INORGANIC/ORGANIC HYBRID MATERIALS WITH ORDERED DOMAINS FOR CHROMATOGRAPHIC SEPARATIONS AND PROCESSES FOR THEIR PREPARATION - Porous hybrid inorganic/organic materials comprising ordered domains are disclosed. Methods of making the materials and use of the materials for chromatographic are also disclosed. | 11-06-2014 |
20140326649 | NOVEL NANOCOMPOSITES AND THEIR APPLICATION AS MONOLITH COLUMNS - Novel materials for chromatographic separations, processes for their preparation, and separation devices containing the chromatographic materials. In particular, hybrid inorganic/organic monolith materials comprising a polymerized scaffolding nanocomposite (PSN), wherein the nanocomposite contains a scaffolding functionally capable of chemically interacting with a surface of a second material are described. The hybrid inorganic/organic materials have enhanced wall adhesion and increased resistance to shrinkage as compared to prior art monolith materials. The improved adhesion of the monoliths enable the preparation of capillary columns with an internal diameter (I.D.)≧50 μm. | 11-06-2014 |
20140319334 | SCREENING FOR PHTHALATES IN FOOD SAMPLES - A method includes the steps of dipping a capillary (e.g., a disposable glass capillary) into a food matrix to deposit a food sample on the capillary; attaching the capillary onto an Atmospheric Solids Analysis Probe (ASAP); loading the ASAP into an atmospheric pressure ionization (API) source enclosure; ionizing the food sample on the capillary within the API source enclosure; and analyzing the ionized sample for the presence of one or more phthalates. | 10-30-2014 |
20140319057 | CHROMATOGRAPHIC MATERIALS FOR THE SEPARATION OF UNSATURATED MOLECULES - The present disclosure relates to a method of separating a compound of interest, particularly unsaturated compound(s) of interest, from a mixture. The compound is separated using a column having a chromatographic stationary phase material for various different modes of chromatography containing a first substituent and a second substituent. The first substituent minimizes compound retention variation over time under chromatographic conditions. The second substituent chromatographically and selectively retains the compound by incorporating one or more aromatic, polyaromatic, heterocyclic aromatic, or polyheterocyclic aromatic hydrocarbon groups, each group being optionally substituted with an aliphatic group. | 10-30-2014 |
20140319042 | ELECTROSPRAY INTERFACE TO A MICROFLUIDIC SUBSTRATE - An apparatus for chemical separations includes a microfluidic substrate having an outlet aperture for outputting an eluent of a sample, a spray unit having an inlet to receive the eluent and an outlet to emit a spray of the eluent, and a force-applying unit. The spray unit has a deformable portion defining the inlet and having an elastic modulus that is lower than an elastic modulus of the microfluidic substrate. The force-applying unit, such as a spring, is disposed to urge the deformable portion in contact with the substrate to form a substantially fluid-tight seal. | 10-30-2014 |
20140306105 | LIQUID CHROMATROGRAPHY SYSTEMS AND METHODS - One aspect of the invention provides a liquid chromatography system including: a first solvent manager configured to dispense various ratios of a first solvent and a second solvent; a first column in fluid communication with the first solvent manager; a mixer in fluid communication with the first column; a first valve in fluid communication with the mixer; a second column having a first end in fluid communication with a first port of the first valve and a second end in fluid communication with a second port of the first valve; a second solvent manager adapted and configured to dispense various ratios of a third solvent and a fourth solvent; and a second valve in fluid communication with the second solvent manager, the first valve, and the mixer. The first valve and the second valve are adapted and configured for actuation between and a second position. In the first position: solvent dispensed by the first solvent manager and an injected sample flow over the first column; eluent from the first column is mixed with solvent dispensed by the second solvent manager in the mixer to produce a combined mobile phase; and the combined mobile phase is passed through the first valve and over the second column in a first direction to trap analytes of interest on the first column. In the second position, solvent dispensed by the second solvent manager is passed over the second column in a second direction to release the analytes of interest from the second column. | 10-16-2014 |
20140301911 | MECHANICALLY SUPPORTING MICROFLUIDIC DEVICES - A microfluidic assembly includes a planar microfluidic separation device and a support body configured to receive the planar microfluidic separation device therein. The support body is configured to apply a substantially distributed compressive preload to a substrate of the planar microfluidic separation device. The compressive preload applied to the planar microfluidic separation device may increase the achievable operating pressure of the planar microfluidic separation device. | 10-09-2014 |
20140284259 | POROUS INORGANIC/ORGANIC HYBRID PARTICLES FOR CHROMATOGRAPHIC SEPARATIONS AND PROCESS FOR ITS PREPARATION - Novel material for chromatographic separations, processes for its preparation, and separations devices containing the chromatographic material. In particular, the disclosure describes porous inorganic/organic hybrid particles having a chromatographically-enhancing pore geometry, which desirably may be surface modified, and that offer more efficient chromatographic separations than that known in the art. | 09-25-2014 |
20140242709 | RAPID FLUORESCENCE TAGGING OF GLYCANS AND OTHER BIOMOLECULES WITH ENHANCED MS SIGNALS - Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described. | 08-28-2014 |
20140239171 | TECHNIQUES FOR AUTOMATED PERFORMANCE MAINTENANCE TESTING AND REPORTING FOR ANALYTICAL INSTRUMENTS - Techniques are described for performing performance maintenance on a mass spectrometer. Pre-maintenance testing is performed that automating execution of a test sequence in response to a first user interface selection. The maintenance activity performed upon completion of said pre-maintenance testing. Post-maintenance testing is preformed upon completion of said maintenance activity. The post-maintenance testing includes automating execution of the test sequence in response to a second user interface selection. A benchmark comparison is performed to determine whether performance of the mass spectrometer has degraded as a result of performing the maintenance activity, wherein said benchmark comparison is performed automatically in response to completing said post-maintenance testing. | 08-28-2014 |
20140230528 | TURBULENT FLOW MIXING DEVICE FOR USE IN A CHROMATOGRAPHY SYSTEM - A mixing device for use in a chromatography system, the device includes an exterior housing having a first end and a second end and a hydraulic flow connector at the first end of the exterior housing. A cartridge including a chamber is enclosed within the exterior housing. The chamber has at least one wall defining an interior volume having a shape, wherein the shape of the interior volume creates a turbulent flow condition to mix at least two fluids and provide flow through the cartridge during operation of the chromatography system. The chamber also retains the sample, thereby ensuring that the sample is focused in the chamber such that a more narrow bolus of sample enters the chromatography column. | 08-21-2014 |
20140220278 | Porous Material And Devices For Performing Separations, Filtrations, And Catalysis And EK Pumps, And Mthods Of Making And Using The Same - Embodiments of the present invention are directed to a porous monolith polymeric composition having utility in catalysis, chromatography, filtration, and electro-kinetic pumps, devices incorporating such composition and methods or making and using such monoliths. The monoliths are characterized by a substantially homogeneous skeletal core with little shrinkage, few voids and few channels. | 08-07-2014 |
20140217196 | ELECTROSPRAY EMITTER ASSEMBLIES FOR MICROFLUIDIC CHROMATOGRAPHY APPARATUS - An apparatus for chemical separations includes a microfluidic substrate having an outlet aperture for outputting an eluent of a sample. An emitter assembly includes having a deformable end portion, an inlet near the deformable end portion to receive the sample eluent from the microfluidic substrate, and an electrically conductive outlet portion to emit a spray of the sample eluent. A force-applying unit applies a force to the emitter assembly that urges the deformable end portion into contact with the microfluidic substrate. The deformable end portion is more elastic than the microfluidic substrate so that the contact between the microfluidic substrate and the deformable end portion produces a substantially fluid-tight seal between the outlet aperture of the microfluidic substrate and the inlet of the emitter assembly. | 08-07-2014 |
20140217031 | METHODS AND DEVICES FOR OPEN-BED ATMOSPHERIC COLLECTION FOR SUPERCRITICAL FLUID CHROMATOGRAPHY - A supercritical fluid chromatography system comprises a first pump for pumping a first flow stream comprising a compressible fluid and a second pump for pumping a second flow stream comprising a modifier fluid. The second pump is in parallel with the first pump. A column is located in a combined flow stream. The column is located downstream of the first and second pumps. The combined flow stream comprises the first flow stream, the second flow stream, and a sample. A detector is located downstream of the column. A gas-liquid separator is located downstream of the detector. The gas-liquid separator is configured to vent a majority of the compressible fluid while maintaining a majority of the sample, thus preventing aerosolization of the flow stream and minimizing sample loss as well as cross contamination. An open bed collector is located after the gas-liquid separator. | 08-07-2014 |
20140208836 | ANALYSIS OF DRIED BLOOD SPOT SAMPLES IN A MICROFLUIDIC SYSTEM WITH DILUTION OF EXTRACTED SAMPLES - An apparatus for use in a chromatography system includes a microfluidic substrate having a fluidic channel configured as an analytical chromatographic column and a fluidic port on one side of the microfluidic substrate. The fluidic port opens at a head end of the analytical chromatographic column. A dried blood spot (DBS) collection device holds one or more dried biological samples. The DBS collection device is directly coupled to the microfluidic substrate whereby one of the biological samples is placed into fluidic communication with the fluidic channel of the microfluidic substrate and an extraction of that biological sample flows toward the head end of the analytical chromatographic column. A diluent source fluidically coupled to the fluidic port supplies a solvent to the head end of the analytical column to dilute the extracted biological sample before the biological sample flows into the analytical chromatographic column. | 07-31-2014 |
20140208834 | Rotary Rheometer With Dual Read Head Optical Encoder - A rotary rheometer includes a drive shaft and a dual read head optical encoder that is configured to measure angular displacement or angular velocity of the drive shaft. | 07-31-2014 |
20140202236 | Maintaining A Measurement Gap In A Rheometer - A rheometer includes a drive shaft, a drag cup motor for rotating the drive shaft, a first measuring object supported by the drive shaft, a second measuring object, a linear position sensor, and processing and control electronics. The linear position sensor includes a target (e.g., an aluminum target) mounted to the drive shaft, and a pair of coils. The linear position sensor is configured to measure thermal expansion of the drive shaft based on a change in impedance of the coils resulting from a displacement of the target relative to the coils. The processing and control electronics are in communication with the coils and are configured to adjust a position of one of the measuring objects relative to the other based on a change in impedance of the coils resulting from a displacement of the target relative to the coils, thereby to maintain a substantially constant measurement gap therebetween. | 07-24-2014 |
20140194283 | POROUS INORGANIC/ORGANIC HOMOGENOUS COPOLYMERIC HYBRID MATERIALS FOR CHROMATOGRAPHIC SEPARATIONS AND PROCESS FOR THE PREPARATION THEREOF - The present invention relates to porous inorganic/organic homogenous copolymeric hybrid material materials, including particulates and monoliths, methods for their manufacture, and uses thereof, e.g., as chromatographic separations materials. | 07-10-2014 |
20140192350 | Flow Cells For Differential Refractive Index Detection - A flow cell for differential refractive index detection. The flow cell includes a transparent body that extends from a first end to a second end along a longitudinal axis. The transparent body defines a sample prism chamber and reference prism chamber. The sample prism chamber is configured to allow fluid flow between the first and second ends of the transparent body along the longitudinal axis. The reference prism chamber is configured to receive a reference fluid. The sample and reference prism chambers each include a grating comprising a plurality of grooves extending along the longitudinal axis in the direction of fluid flow. | 07-10-2014 |
20140191146 | Rotary Shear Valve with a Two-pin Drive Shaft for Liquid Chromatography Applications - A rotary shear valve assembly for liquid chromatography applications comprises a rotor assembly having a rotor and a drive shaft with a head portion. The rotor has a substantially planar surface with one or more rotor grooves and a pair of holes. The head portion has two pins. The pins are disposed substantially diametrically opposite of each other on a line through a center of the head portion. Each pin mates with one of the holes in the rotor. The rotor assembly can further comprise means for urging the rotor surface against the stator surface such that each rotor groove aligns with and provides a fluidic channel between two of the stator openings. | 07-10-2014 |
20140190890 | COLLECTION SYSTEM FOR PURIFICATION FLOWSTREAMS - A collection system for collecting samples from a flowstream ( | 07-10-2014 |
20140179011 | RAPID FLUORESCENCE TAGGING OF GLYCANS AND OTHER BIOMOLECULES WITH ENHANCED MS SIGNALS - Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described. | 06-26-2014 |
20140178915 | METHOD FOR SEPARATING PEPTIDES AND PROTEINS - Embodiments of the present invention are directed to articles of manufacture, devices, methods and apparatus for performing liquid chromatography featuring a chromatographic sorbent having one or more pentafluorophenyl groups, wherein said one or more pentafluorophenyl groups are a bonded phase on a sorbent selected from the group comprising silica, organic polymers or hybrid organic silane material and said pentafluorophenyl groups are in a mono-, bi-, and tridentate forms. | 06-26-2014 |
20140174541 | ROTARY SHEAR VALVE AND ASSOCIATED METHODS - Exemplary embodiments are directed to rotary shear valves which include a stator, a rotor defining a cavity extending at least partially therethrough, and a bladder. The rotor is rotatably mounted relative to the stator to create at least one fluidic path therebetween. The bladder comprises a polymer disposed inside the cavity. Exemplary embodiments are also directed to methods of operating a rotary shear valve. | 06-26-2014 |
20140174160 | MICROFLUIDIC DEVICE WITH DRIED BLOOD SPOTS (DBS) CARD INTERFACE - An apparatus for use in a chromatography system includes a first microfluidic substrate having a first fluidic channel. One end of the first fluidic channel terminates at a first fluidic port on a first side of the first microfluidic substrate and an opposite end of the first fluidic channel terminates at a second fluidic port on a second side of the first microfluidic substrate. A second microfluidic substrate has a second fluidic channel. One end of the second fluidic channel terminates at a first fluidic port on a first side of the second microfluidic substrate. The first side of the second microfluidic substrate abuts the second side of the first microfluidic substrate such that the fluidic port of the second microfluidic substrate aligns with one of the fluidic ports of the first microfluidic substrate and the alignment produces a fluidic path comprised of the first and second fluidic channels. | 06-26-2014 |
20140166581 | POROUS HYBRID MONOLITH MATERIALS WITH ORGANIC GROUPS REMOVED FROM THE SURFACE - A material for chromatographic separations, processes for its preparation, and separation devices containing the chromatographic material. In particular, porous inorganic/organic hybrid monoliths are provided with a decreased concentration of surface organic groups, and have improved pH stability, improved chromatographic separation performance, and improved packed bed stability. These monoliths may be surface modified resulting in higher bonded phase surface concentrations and have enhanced stability at low pH. | 06-19-2014 |
20140166562 | CHROMATOGRAPHY COLUMN ASSEMBLY - Described is a chromatography column assembly that includes a permanently deformable outer tube, an intermediate tube, an inner tube and a sorbent bed disposed within the inner tube. The sorbent bed may be in the form of packed chromatographic particles or a porous monolithic structure. A radial seal is provided by one or more uniform radial crimps at longitudinal locations on the assembly. The uniform radial crimp compresses the outer tube and underlying intermediate tube onto the inner tube to achieve a high pressure liquid tight seal between the three tubes. The length and depth of each crimp is accurately formed to accommodate the requirements of the particular application. Leakage along the tubing assembly is prevented and void volume is reduced or eliminated. No external ferrule or ferrule swaging mechanism is needed; therefore the chromatography column assembly is easily adapted for use in various chromatographic column configurations. | 06-19-2014 |
20140162298 | IMMOBILIZED ENZYMATIC REACTOR - An immobilized enzymatic reactor can include a wall defining a chamber having an inlet and an outlet; a solid stationary phase covalently linked to an enzyme and disposed within the chamber; and a pressure modulator in fluid communication with the chamber and adapted to support continuous flow of a liquid sample comprising a polymer analyte through the inlet, over the solid stationary phase, and out of the outlet under a pressure between about 2,500 and 35,000 psi. In one example, the solid stationary phase includes inorganic/organic hybrid particles in an ultra performance liquid chromatography system, the enzyme is a protease, and the polymer analyte is a polypeptide. The immobilized enzymatic reactor can prepare an analyte for applications such as for hydrogen deuterium exchange mass spectrometry. | 06-12-2014 |
20140162248 | Use of Aptamers in Liquid Chromatography and Liquid Chromatography - Mass Spectrometry - The present disclosure relates to the use of aptamers in solid phase extraction, chromatography and chromatography-mass spectrometry systems. More specifically, the present disclosure relates to the use of aptamers in SPE and chromatography systems to selectively retain, extract and/or pre-concentrate target molecule(s) having a specific affinity for the particular aptamer(s). The target molecule(s) can be further analyzed by mass spectrometry with limited interferences and/or enhanced sensitivity. | 06-12-2014 |
20140158881 | COMPOSITIONS, METHODS, AND KITS FOR QUANTIFYING TARGET ANALYTES IN A SAMPLE - A method of quantifying a target analyte by mass spectrometry includes obtaining a mass spectrometer signal comprising a first calibrator signal, comprising a second calibrator signal, and potentially comprising a target analyte signal from a single sample comprising a first known quantity of a first calibrator, comprising a second known quantity of a second calibrator, and potentially comprising a target analyte. The first known quantity and the second known quantity are different, and wherein the first calibrator, the second calibrator, and the target analyte are each distinguishable in the single sample by mass spectrometry. The method also includes quantifying the target analyte in the single sample using the first calibrator signal, the second calibrator signal, and the target analyte signal. | 06-12-2014 |
20140158599 | LIQUID CHROMATOGRAPHY CONDUIT ASSEMBLIES HAVING HIGH PRESSURE SEALS - Described is a tubing assembly which includes a permanently deformable outer tube, an intermediate tube and an inner tube. A radial seal is provided by a uniform radial crimp having a non-zero longitudinal length at a longitudinal location on the tubing assembly. In some embodiments one or both ends of the assembly have a uniform radial crimp and are polished so that the ends of two or more of the tubes are substantially flush with each other to thereby block the passage of fluids between the ends of the tubes. In other embodiments the uniform radial crimps are at other longitudinal locations where a high pressure seal is desired. The longitudinal length and the depth of each uniform radial crimp can be formed to accommodate the requirements of a particular application so that leakage along the tubing assembly is prevented and void volume is reduced or eliminated. | 06-12-2014 |
20140157988 | CHROMATOGRAPHY APPARATUS HAVING DIFFUSION BONDED COUPLER - Described is a chromatography a chromatography apparatus that includes a microfluidic substrate, a coupler and a sealing fitting. The microfluidic substrate can include one or more chromatography columns, such as analytical columns or trap columns, and an inlet in communication with the one or more chromatography columns. The coupler is diffusion bonded to the surface of the microfluidic substrate so that an opening in the coupler is aligned to the inlet in the microfluidic substrate. The sealing fitting includes a heat-resistant sealant disposed in the opening of the coupler and in contact with the surface of the substrate. The coupler and sealing fitting enable a compact fluid-tight connection to the inlet of the microfluidic substrate that exhibits high tensile and torsional strength, and does not require the use of adhesives. | 06-12-2014 |
20140157871 | SYSTEM AND METHOD FOR RAPID ANALYSIS OF POLYMER ADDITIVES - The subject technology is directed to a CO | 06-12-2014 |
20140152003 | REUSABLE FITTING FOR ATTACHING A CONDUIT TO A PORT - Described is a reusable fitting for attaching a conduit to a port. The fitting includes a housing, a spring, a retainer and cap. The retainer is capable of locking and unlocking a conduit in the fitting. The fitting provides a high pressure fluid-tight seal and a substantially zero void volume at the junction of two fluidic conduits. The fitting is removable, reusable and replaceable, and allows a user to make and remake connections of a capillary to a fluidic port without permanently binding a ferrule to a specific capillary and thus to a specific port. The fitting can be replaced if part or all of the fitting is damaged during use. | 06-05-2014 |
20140151541 | Electrokinetically Controlled Calibrant Delivery - An electrokinetic pump can be used to deliver calibrant (“lock mass”) ions to a mass spectrometer for calibration of a mass spectrometry system. Electrokinetically controlled calibrant delivery can help to eliminate the need for the more cumbersome mechanisms that are often used for ion delivery. In addition, electrokinetically controlled calibrant delivery can provide for a more user-friendly system in which a calibrant solution can be packaged into a disposable cartridge. Furthermore, when implemented in a microfluidic format, electrokinetically controlled calibrant delivery can be coupled with an electrokinetically controlled separation system, such as capillary electrophoresis (CE), to allow efficient solid-state switching between analytical and calibrant sprays. | 06-05-2014 |
20140150536 | COLUMN MANAGER WITH A MULTI-ZONE THERMAL SYSTEM FOR USE IN LIQUID CHROMATOGRAPHY - A thermal system for use in a column manager of a liquid chromatography system comprises a plurality of spatially separated individually controlled thermoelectric chips. A column module houses a plurality of thermally conductive troughs. Each trough resides in a separate thermal zone to be thermally conditioned individually by one of the individually controlled thermoelectric chips. Each trough is adapted to hold one or more liquid chromatography columns therein. A plurality of spatially separated thermal bridges includes a first thermal bridge thermally coupling one of the thermoelectric chips to a first one of the plurality of troughs and a second thermal bridge of the plurality of thermal bridges thermally coupling another of the thermoelectric chips to a second one of the plurality of troughs. | 06-05-2014 |
20140138541 | Dual Source Mass Spectrometry System - A dual source mass spectrometer system ( | 05-22-2014 |
20140138312 | Reducing Dispersion Due To Vias In Planar Microfluidic Separation Devices - A planar microfluidic chemical separation device includes a separation channel that is located in the plane of the device. The device also includes one or more vias situated perpendicular to the separation channel. The vias extends between the separation channel and an outer surface of the substrate for fluid communication with the separation channel. The vias have cross-sectional areas that are substantially less than a cross-sectional area of a first region of the separation channel to inhibit band-broadening caused by passage of a sample band through the one or more vias. | 05-22-2014 |
20140137967 | Valves Having Protective Coatings - A shear valve for use in a high performance liquid chromatography system. The shear valve includes a first valve member having a plurality of first fluid-conveying features, and a second valve member having one or more second fluid-conveying features. The second valve member is movable, relative to the first valve member, between a plurality of discrete positions such that, in each of the discrete positions, at least one of the one or more second fluid-conveying features overlaps with multiple ones of the first fluid conveying features to provide for fluid communication therebetween. At least one of the first and second valve members is at least partially coated with a protective coating that includes an adhesion interlayer and a diamond-like carbon (DLC) layer. The DLC layer is deposited on the adhesion interlayer via filtered cathodic vacuum arc (FCVA) deposition. | 05-22-2014 |
20140131277 | Closed Loop Flow Control Of A HPLC Constant Flow Pump To Enable Low-Flow Operation - A method and apparatus for monitoring and controlling nano-scale flow rate of fluid in the operating flow path of a HPLC system provide fluid flow without relying on complex calibration routines to compensate for solvent composition gradients typically used in HPLC. The apparatus and method are used to correct the flow output of a typical, analytical-scale (0.1-5 mL/min) HPLC pump to enable accurate and precise flow delivery at capillary (<0.1 mL/min) and nano-scale (<1 μL/min) HPLC flow rates. | 05-15-2014 |
20140110933 | LOW CARRYOVER HIGH PRESSURE FLUIDIC FITTING - Described is a fitting for coupling fluidic paths which facilitates secure and low leak rate fluid flow through a fluidic path junction such as a junction in a liquid chromatography system or other chemical analysis instrument. Fluid containing various mixtures of chemical samples can pass through the junction at high pressure with little or no trapped residue remaining in a volume at the connection. According to various embodiments, a face seal is achieved between two separate fluidic path features. In some embodiments, the sealing of the fluidic paths is aided by the use of a compliant polymer seal. The seal facilitates intimate contact between adjacent surfaces and significantly limits or prevents the entry of fluid under high pressure into an unswept volume. Thus the risk of carryover and its adverse effect on measurements is reduced or eliminated. | 04-24-2014 |
20140096596 | POROUS MATERIALS FOR SOLID PHASE EXTRACTION AND CHROMATOGRAPHY AND PROCESSES FOR PREPARATION AND USE THEREOF - The invention provides novel porous materials that are useful in chromatographic processes, e.g., solid phase extraction, and that provide a number of advantages. Such advantages include superior wetting characteristics, selective capture of analytes of interest, and non-retention of interfering analytes. The invention advantageously provides novel porous materials having a large percentage of larger pores (i.e. wide pores). The invention advantageously provides novel porous materials that overcome the problems of SPE of biological samples. | 04-10-2014 |
20140086003 | VALVE SWITCH MODULATION FOR REDUCING ERRORS DUE TO OSCILLATIONS OF THE INLET FLUID OF A PUMP SYSTEM - Described is a method of reducing liquid composition errors in a low-pressure mixing pump system. Packets representing the switching intervals of each component of the desired fluid mixture are provided to an intake of the mixing pump system. For each packet, a switching time associated with at least one of the components in the packet is modulated. Modulated switching times are based on time offsets that are specifically selected according to the undesirable frequency characteristic of an intake response of the mixing pump system. The average of the volumes contributed by the packets thus modulated is equal to a component volume that achieves a desired proportion of the component in the output flow of the mixing pump system. Modulated switching times enable the reduction or elimination of composition error in the output flow of the mixing pump system. | 03-27-2014 |
20140079606 | SAMPLE TRAY WITH MAGNETICALLY CLOSING DRAWER - A sample manager of a liquid chromatography system uses a sample tray having a base with side walls separated by a cross wall that divides the base into two compartments. The side walls and cross wall bound each compartment on three sides. A fourth side of each compartment is open. Each compartment is sized to closely receive a sample-vial carrier. Each compartment has a magnet affixed to a bottom surface at an edge of the open fourth side. Each of two drawers slides into the open fourth side of one compartment. Each drawer has a support surface with a magnet affixed at an edge of its bottom side, which aligns with the magnet affixed to the bottom surface of the compartment. The magnet of the drawer and the magnet of the compartment bias the drawer into its compartment when the magnets are brought into proximity of each other. | 03-20-2014 |
20140065651 | DESTRUCTIBLE SURFACTANTS AND USES THEREOF - The present invention provides methods for enhancing chemical reactions of molecules, e.g., biomolecules, with destructible surfactants. The chemical reactions may involve and/or be associate with analysis, e.g., solubilizing, separating, purifying and/or characterizing the molecules. In one aspect, the anionic surfactants of the present invention may be selectively broken up at relatively low pH. The resulting breakdown products of the surfactants may be removed from the molecule/sample with relative ease. The invention has applicability in a variety of analytical techniques. | 03-06-2014 |
20140053910 | HIGH PRESSURE FLUIDIC SWITCHING VALVE HAVING VARIABLE PRESSURE LOADING - Described are a rotary shear seal valve and a method for switching a high pressure fluid. The method includes applying a fixed force between a planar surface of a rotor and a planar surface of a stator. The planar surface of the rotor includes a fluidic channel and the planar surface of the stator has a pair of ports to receive and provide the fluid. A control signal is applied to a linear actuator coupled to the rotor to generate a controllable force between the planar surfaces of the rotor and stator. The control signal is responsive to a rotational state of the rotor. The total force between the planar surfaces of the rotor and the stator is substantially equal to a sum of the fixed force and the controllable force. The method reduces the wear and extends the lifetime of components in a rotary shear seal valve. | 02-27-2014 |
20140053639 | Fitting Assemblies - A fitting assembly includes a first fitting that is configured to receive a first fluid tube and a second fitting that is configured to receive a second fluid tube. The first fitting defines a first groove. The second fitting includes a spring that is configured to engage the first groove to connect the first and second fittings such that the first and second fluid tubes are placed in fluid communication and a fluid tight seal is established between the first and second sealing fittings. | 02-27-2014 |
20140047907 | Cartridge with Multiple Electrospray Emitters - An apparatus includes a portable housing and a plurality of separately operable microfluidic emitters disposed within the housing. Each microfluidic emitter h an inlet for receiving a liquid and an outlet for emitting a spray. Each microfluidic emitter is movable within the portable housing between a first position in which the outlet of that microfluidic emitter is fully retracted within the portable housing and a second position in which the outlet of that microfluidic emitter projects out of the portable housing. The portable housing with the multiple emitters is adapted to be detachably coupled to another housing portion. This housing portion includes a microfluidic substrate with a channel for transporting an eluent and an outlet aperture for emitting the eluent. The inlet of one of the emitters is detachably coupled to the outlet aperture of the microfluidic substrate. | 02-20-2014 |
20140047905 | Electrospray Emitter Assembly - An electrospray emitter assembly ( | 02-20-2014 |
20140044577 | HIGH PRESSURE PUMP WITH REDUCED SEAL WEAR - Described are embodiments of a pump that can be used, for example, in liquid chromatography applications. The pump includes a seal wash housing, pump head and seal assembly. A wear-resistant coating applied to a sealing surface or gland of the pump head or seal wash housing improves the hardness and chemical compatibility of the sealing surface. Oxidation of the sealing surface is reduced so that the sealing surface is less abrasive to the seal assembly and the lifetime of the seal assembly is increased. | 02-13-2014 |
20140038217 | METHOD AND APPARATUS FOR FRACTIONATION-BASED CHEMICAL ANALYSES | 02-06-2014 |
20140038216 | METHOD AND APPARATUS FOR PERFORMING RETENTION TIME MATCHING - A method for matching a precursor ion with one or more related product ions includes providing input data sets obtained from sample injections, each of the data sets including a precursor ion and one or more product ions, normalizing the input data sets in accordance with a single retention time for the precursor ion, determining which product ions are within a predetermined retention time window with respect to the single retention time, and, if a product ion is within the predetermined retention time window for a specified number of the input data sets, determining that the product ion is related to the precursor having the single retention time. An apparatus for analyzing a sample includes a chromatography module, a mass-spectrometry module in communication with the chromatography module, and control unit in communication with the chromatography module and the mass-spectrometry module. | 02-06-2014 |
20140034826 | SYSTEM AND METHOD FOR GROUPING PRECURSOR AND FRAGMENT IONS USING SELECTED ION CHROMATOGRAMS - LC/MS data generated by an LC/MS system is analyzed to determine groupings of ions associated with originating molecules. Ions are grouped initially according to retention time, for example, using retention time or chromatographic peaks in mass chromatograms. After initial groupings are determined based on retention time, ion peak shapes are compared to determine whether ions should be excluded. Ions having peak shapes not matching other ions, or alternatively a reference peak shape, are excluded from the group. | 02-06-2014 |
20140033793 | CHROMATOGRAPHIC SYSTEM QUALITY CONTROL REFERENCE MATERIALS - The invention provides compositions and methods for chromatographic analysis and system quality control. Compositions can include a reference material that comprises a standardized formulation of two or more compounds that can be used for benchmarking and troubleshooting the chromatographic system (e.g., which is not simply a standard solution for a particular analyte of interest). Methods can include obtaining a chromatogram from a reference material using a chromatographic system, wherein the reference material comprises a standardized formulation of two or more compounds that can be used for benchmarking and troubleshooting the chromatographic system; evaluating a difference between the chromatogram and a benchmark for the reference material on the chromatography system; and (i) analyzing a sample potentially comprising an analyte of interest, wherein the analyte of interest does not comprise any of the two or more compounds of the standardized formulation, or compounds substantially similar thereto, if the difference between the chromatogram and the benchmark is within a predetermined tolerance, or (ii) troubleshooting the chromatography system using the chromatogram if the difference between the chromatogram and the benchmark is not within the predetermined tolerance. | 02-06-2014 |
20140025342 | ION DETECTION AND PARAMETER ESTIMATION FOR N-DIMENSIONAL DATA - Methods and apparatus for LC/IMS/MS analysis involve obtaining noisy raw data from a sample, convolving the data with an artifact-reducing filter, and locating, in retention-time, ion mobility, and mass-to-charge-ratio dimensions, one or more ion peaks of the convolved data. | 01-23-2014 |
20140014585 | CHROMATOGRAPHIC INTERFACE - An apparatus for chromatographic interfacing includes an interfacing unit, a sample acquisition unit, and a sample trapping unit. The interfacing unit includes (i) a chromatographic inflow valve adapted to receive a chromatographic sample separation flow, (ii) a chromatographic outflow valve adapted to allow discharge of the sample separation flow, (iii) a loop expulsion inflow valve adapted to receive an expulsion fluid flow, and (iv) a loop expulsion outflow valve in fluid communication with the loop expulsion inflow valve and adapted to allow discharge of a fluid. The sample acquisition unit includes (i) an inflow selector in selectable fluid communication with the chromatographic inflow valve or the loop expulsion inlet valve, (ii) an outflow selector in fluid communication with the loop expulsion outflow valve, and (iii) one or more collection loops, each loop being independently selectable to establish fluid communication between the inflow selector valve and the outflow selector valve. The sample trapping unit includes (i) a trap in selectable fluid communication with the loop expulsion outflow valve and defining a flow path from a first port to a second port and through a stationary phase disposed therebetween, the stationary phase adapted to trap an analyte of interest from a fraction of the chromatographic sample separation flow, and (ii) a scavenging gas source in selectable fluid communication with the flow path and a vacuum source in selectable fluid communication with the flow path, the scavenging gas source and vacuum source adapted to substantially dry the stationary phase. | 01-16-2014 |
20130340609 | PUMP HEAD OUTLET PORT - Pumps used in liquid chromatography applications employ actuators for pressurizing and moving liquid. An actuator includes a pump head having a chamber, a gland, a movable rod extending through the gland into the chamber of the pump head, and a plunger seal disposed in the gland around the rod for sealing against fluidic leakage from the chamber. The plunger seal has an annular flange that provides an OD (outer diameter) seal against the gland. The flange defines a seal cavity. The pump head further includes an outlet port and an outlet channel. One end of the outlet channel is in communication with the outlet port and the other end of the outlet channel opens into the seal cavity of the plunger seal. The outlet channel provides a flow path for liquid in the chamber to travel towards the outlet port in response to movement of the rod. | 12-26-2013 |
20130340509 | Flow Through Isolation Valve For High Pressure Fluids - A flow through isolation valve having a stationary member; a movable member, with a surface of the stationary member interfacing with a surface of the movable member; and at least one pin isolation valve. The pin isolation valve has a flow through internal conduit and is movable so that the internal conduit can fluidically communicate with at least one blank opening in the movable member and with a flow through internal conduit in the movable member. Movement is by rotation, linear or curvilinear translation. At least one pin isolation valve is fluidically coupled typically to a sample loop of a face seal valve, or to a pump supplying high pressure liquid to or to a column discharging liquid from a face seal valve of a high pressure liquid chromatography (HPLC) system or directly to the face seal valve. | 12-26-2013 |
20130340503 | Pressure Monitor Optimizaiton Of Fluid Path Utilization - A device comprising a pressure monitor and a control means that receives a signal representing measured pressure at the pressure monitor and controls the controllable elements of a fluid system is utilized to monitor a fluid system for error conditions, to optimize operations and to diagnose the fluid system. By following a testing protocol that selectively enables parts of the system, the control means narrows the list of possible failing components. Comparing the measured pressure against normal pressures allows the device to identify error conditions. Determining the volume of fluid being transported and controlling the duration of the flow optimizes operation of the fluid system. | 12-26-2013 |
20130334117 | SYSTEM AND METHOD OF COOLING A PUMP HEAD USED IN CHROMATOGRAPHY - An actuator has a support plate, a pump head in thermally conductive contact with the support plate, and an actuator body. Cooling means is disposed between the support plate and the actuator body. The cooling means is configured to remove and transfer heat from the pump head to the actuator body. In one embodiment, the cooling means includes two thermally conductive plates (called cold-side and hot-side plates) and a thermoelectric device disposed in thermally conductive contact between the cold-side and hot-side plates. The cold-side plate is in thermal communication with the pump head through the support plate. The hot-side plate is in thermal communication with the actuator body. The thermoelectric device is configured to transfer heat from the cold-side plate, which is in thermal communication with the pump head, to the hot-side plate, which is in thermal communication with the actuator body, thereby removing heat from the pump head. | 12-19-2013 |
20130324395 | POROUS INORGANIC/ORGANIC HYBRID MONOLITH MATERIALS FOR CHROMATOGRAPHIC SEPARATIONS AND PROCESS FOR THEIR PREPARATION - Novel materials for chromatographic separations, processes for their preparation, and separation devices containing the chromatographic materials. In particular, the novel materials are porous inorganic/organic hybrid monolith materials, which desirably may be surface modified, and which offer more efficient chromatographic separations than that known in the art. | 12-05-2013 |
20130323103 | SEAL ASSEMBLIES FOR RECIPROCATING AND ROTARY APPLICATIONS - Low-pressure and high-pressure reciprocating and rotary applications use seal assemblies to prevent leakage. One embodiment of a seal assembly includes a major annular body having opposing spaced-apart annular lips extending from a heel portion with a bore extending through the heel portion. A first spring is disposed between the lips, biasing the lips apart. A minor annular body extends from one of the lips of the major annular body. The minor annular body has opposing spaced-apart walls that extend from a base region and form a pocket. A second spring is disposed in the pocket between the spaced-apart walls, biasing the walls apart. During actuator operation, pressurized fluid urges one wall of the minor annular body against a pump head surface to produce a face seal and one of the lips of the major annular body against a rod surface to produce a radial seal. | 12-05-2013 |
20130319086 | HIGH PURITY CHROMATOGRAPHIC MATERIALS COMPRISING AN IONIZABLE MODIFIER - The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are high purity chromatographic materials comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifier. | 12-05-2013 |
20130308415 | VALVE SWITCH MODULATION FOR REDUCING ERRORS DUE TO OSCILLATIONS OF THE INLET FLUID OF A PUMP SYSTEM - Described is a method of reducing liquid composition errors in a low-pressure mixing pump system. Packets representing the switching intervals of each component of the desired fluid mixture are provided to an intake of the mixing pump system. For each packet, a switching time associated with at least one of the components in the packet is modulated. Modulated switching times are based on time offsets that are specifically selected according to the undesirable frequency characteristic of an intake response of the mixing pump system. The average of the volumes contributed by the packets thus modulated is equal to a component volume that achieves a desired proportion of the component in the output flow of the mixing pump system. Modulated switching times enable the reduction or elimination of composition error in the output flow of the mixing pump system. | 11-21-2013 |
20130276520 | Rotary Shear Injector Valve With Displaced Rotor Grooves - A valve includes a stator that has fluidic ports in a seal surface, and a rotor that has a seal surface contacting the stator's seal surface. The rotor has through-holes that extend from the seal surface to a back surface, providing fluidic communication with a conduit disposed adjacent to the back surface of the rotor. A chromatography apparatus includes a sample source, a solvent source having a higher operating pressure than the sample source, and an injector valve. A method of performing chromatography includes loading a sample with a valve in a load-state configuration that disposes a sample source in fluidic communication with a conduit disposed adjacent to a back surface of the rotor, and injecting the loaded sample with the valve in an inject-state configuration that disposes a solvent source in fluidic communication with a column via at grooves adjacent to a front seal surface of the rotor. | 10-24-2013 |
20130269424 | Chromatographic Optical Detection System - A chromatographic optical detection system includes an optical detector disposed to receive light scattered from a stream of particles and configured to convert the received light to an electrical signal; a signal-processing unit in signal communication with the optical detector to receive the electrical signal, and configured to convert the electrical signal to digital pulses and count the digital pulses to output a first signal corresponding to a number of particles detected in a time interval, and configured to integrate and digitize the electrical signal to output a second signal corresponding to the number of particles detected in the time interval; and a data station in signal communication with the signal-processing unit, and configured to select the first signal, if the number of particles detected in the time interval is less than a threshold criterion, and to select the second signal if the number of particles detected in the time interval exceeds the threshold criterion. The threshold criterion is associated with a saturation condition. | 10-17-2013 |
20130256231 | Device, Apparatus And Method For Performing Separations - Embodiment of the present invention feature a device having a body, a separation media and a frit element. The exterior surface of the body has a first attachment means positioned radially about at least one of the media chamber and the frit section to form a compact assembly. | 10-03-2013 |
20130232474 | TECHNIQUES FOR AUTOMATED SOFTWARE TESTING - Described are techniques for testing an application. A test script including one or more instructions is received to test an application. A user interaction with a user interface of the application is emulated. Emulating includes executing a first portion of instructions in the test script. An output is received including information displayed using the user interface in response to the user interaction. Verification processing is performed for the output. The verification processing includes executing a second portion of instructions in the test script. A first instruction in the test script references a user interface data element without specifying a complete path for the user interface data element. | 09-05-2013 |
20130228513 | GRADIENT SEPARATION METHOD TRANSFER FOR LIQUID CHROMATOGRAPHY SYSTEMS - Described is a method of transferring a gradient separation method to a liquid chromatography system. A value of a delivered gradient slope for a gradient separation method performed on a first liquid chromatography system is determined based on a value of a programmed gradient slope for the gradient separation method and a predetermined relationship between delivered gradient slope and programmed gradient slope for the first liquid chromatography system. The gradient separation method is performed on a second liquid chromatography system using a delivered gradient slope having a value equal to the value of the delivered gradient slope determined for the first liquid chromatography system | 09-05-2013 |
20130208759 | Thermal Analysis Sample Holder - A sample holder for use in a scientific instrument requiring accurate and precise measurements of temperatures. The sample holder includes a ceramic sample cup diffusion bonded to a ceramic beam or to a ceramic adapter. A thermocouple is welded to the bottom of the sample cup. | 08-15-2013 |
20130206666 | Process For Preparing Liquid Mixtures Of Known pH And Salt Concentration - A method of preparing a liquid mixture for use in a liquid chromatography system is provided. The mixture comprises one or more acids, one or more bases, one or more salts, and one or more solvents, and the method comprises the steps of: i) calculating pH and/or salt concentration at a particular time t from a user-determined gradient function; and ii) based on the values obtained in step (i), calculating percent acid, percent base, percent salt and percent solvent in the liquid mixture at time t. A liquid chromatography system incorporating such method is also provided. | 08-15-2013 |
20130206665 | SUPERFICIALLY POROUS MATERIALS COMPRISING A SUBSTANTIALLY NONPOROUS HYBRID CORE HAVING NARROW PARTICLE SIZE DISTRIBUTION; PROCESS FOR THE PREPARATION THEREOF; AND USE THEREOF FOR CHROMATOGRAPHIC SEPARATIONS - The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are chromatographic materials comprising having a narrow particle size distribution. | 08-15-2013 |
20130206240 | VARIABLE-VOLUME INJECTION VALVE - Variable-volume injection valves include a stator and a rotor. The stator has a first port, a second port, and a contact surface with a groove therein. The first port opens into the stator groove. The rotor has a contact surface with a groove therein. The contact surface of the rotor is urged against the contact surface of the stator such that the rotor groove opposes the stator groove with one end of the rotor groove overlapping the stator groove and the opposite end of the rotor groove overlapping the second port of the stator. The overlapping grooves of the rotor and stator provide a fluidic channel between the first and second ports of the stator. The rotor is movable with respect to the stator in order to vary a length of overlap between their overlapping grooves. | 08-15-2013 |
20130195949 | Antimicrobial Layer For Chromatographic Containers - A chromatographic container ( | 08-01-2013 |
20130177039 | LARGE ARRAY DIFFERENTIAL SCANNING CALORIMETER, DSC MEASURING UNIT - Embodiments of the present invention feature a method and apparatus for an energetics-based approach to screen and to characterize binding interactions between potential therapeutic (or diagnostic) agents and unknown target molecules. The methods and apparatus detect the occurrence of these reactions, the strength of the binding interaction and possibly the rate at which these processes take place. | 07-11-2013 |
20130151190 | TECHNIQUES FOR AUTOMATED INSTALLATION TESTING AND REPORTING FOR ANALYTICAL INSTRUMENTS - Automated installation processing of a mass spectrometer is described. Software is executed providing a user interface for controlling the installation process. Manual setup operations in connection with physical installation of the mass spectrometer are performed. Instrument level testing of the mass spectrometer is performed. The instrument level testing includes automating execution of a first test sequence in response to a first user interface selection. The first test sequence includes one or more performance tests whereby mass spectral data characterizing observed performance of the mass spectrometer is compared to predetermined performance criteria. System level testing of functionality of the mass spectrometer in combination with one or more other components is performed upon successful completion of said instrument level testing. The system level testing includes automating execution of a second test sequence in response to a second user interface selection. System level testing is performed after successful completion of instrument level testing. | 06-13-2013 |
20130148461 | Apparatus And Methods For Controlling The Composition Of Fluids In A Fluid Stream - Embodiments of the present invention are directed to a devices and methods for forming a desired miture of two or more fluids in a conduit. The devices and methods feature control means having error monitoring means and in response to an error event the control means organizes the packet-group of command signals to form at least one first error packet group. The error event occurs during the period of time in which the at least one error packet-group is received by a selectable value. The at least one error packet-group comprising an order or timing of first command signals and second commands signals to minimize deviation from the desired mixture in the flow of mixture-packets forming the desired mixture of two or more fluids in a conduit during the error event. | 06-13-2013 |
20130135610 | DEVICE AND METHODS FOR PERFORMING SIZE EXCLUSION CHROMATOGRAPHY - The present invention is directed to a device and a method for performing size exclusion chromatography. Embodiments of the present invention feature devices and methods for size exclusion chromatography at normal high performance liquid chromatography or ultra performance liquid chromatography pressures and above using small particles. | 05-30-2013 |
20130134083 | Liquid-Chromatography Conduit Assemblies Having High-Pressure Seals - A method for making a liquid-chromatography apparatus includes inserting two inner conduits into an intermediate tube, inserting the intermediate tube into an outer tube, forming a proximal seal between the intermediate tube and at least one of the inner conduits, and forming a distal seal between the intermediate tube and at least one of the inner conduits. A liquid-chromatography apparatus includes an outer tube, an intermediate tube disposed in the outer tube, two inner conduits disposed in the intermediate tube, a proximal seal between the intermediate tube and at least one of the inner conduits, and a distal seal between the intermediate tube and at least one of the inner conduits. | 05-30-2013 |
20130134079 | MOBILE PHASE SOLVENT DELIVERY MODULE - Described is a mobile phase solvent delivery module for liquid chromatography and supercritical fluid chromatography systems. The module includes a pump disposed in a fluid path that conducts a mobile phase solvent. The pump provides the mobile phase solvent at an increased pressure. The module also includes a purification cartridge disposed in the fluid path either before or after the pump. The purification cartridge has an inlet to receive the mobile phase solvent and an outlet to provide a purified mobile phase solvent, and includes a packing material for removing an impurity from the mobile phase solvent. In various embodiments, the module also includes one or more additional components to allow various techniques for regeneration or replacement of spent purification cartridges. For example, the module may also include one or more of a temperature control module, a fluidic switch and one or more additional purification cartridges. | 05-30-2013 |
20130133760 | PRESSURE SENSING AND FLOW CONTROL IN DIFFUSION-BONDED PLANAR DEVICES FOR FLUID CHROMATOGRAPHY - Flow through pressure sensors for use in fluid chromatography systems include a planar device formed from diffusion bonding of a plurality of metallic sheets and at least one sensing element. The planar device has a top surface, a bottom surface and a flow through channel. A diaphragm formed from a portion of one of the top or bottom surfaces is located adjacent to a sensing region of the flow through channel and is attached to the sensing element. The diaphragm is sized to deflect a distance in response to fluid pressure in the sensing region, which has an internal volume of less than about 25 microliters. The diaphragm and attached sensing element form a pressure sensor that measures strain or deflection of the diaphragm to calculate a pressure within the sensing region. | 05-30-2013 |
20130126721 | Measurement Of 25-Hydroxyvitamin D3 And C3 EP1-25-Hydroxyvitamin D3 - The invention describes a method of quantification of an analyte selected from the group consisting of 25-hydroxyvitamin D3 and C3-epi-25-hydroxyvitamin D3 in a specimen containing the analyte; comprising the steps of subjecting the specimen to UPLC reverse-phase separation; and; detecting the protonated precursor pseudo-molecular ion of the analyte using a mass spectrometry technique to determine the amount of the analyte. | 05-23-2013 |
20130118706 | Inspection Method And Device With Heat Exchangers - An inspection device and method, the device comprising a thermoelectric module ( | 05-16-2013 |
20130112605 | SUPERFICIALLY POROUS MATERIALS COMPRISING A SUBSTANTIALLY NONPOROUS CORE HAVING NARROW PARTICLE SIZE DISTRIBUTION; PROCESS FOR THE PREPARATION THEREOF; AND USE THEREOF FOR CHROMATOGRAPHIC SEPARATIONS - Novel chromatographic materials for chromatographic separations, columns, kits, and methods for preparation and separations with a superficially porous material comprising a substantially nonporous core and one or more layers of a porous shell material surrounding the core. The material of the invention is comprised of superficially porous particles and a narrow particle size distrution. The material of the invention is comprised of a superficially porous monolith, the substantially nonporous core material is silica; silica coated with an inorganic/organic hybrid surrounding material; a magnetic core material; a magnetic core material coated with silica; a high thermal conductivity core material; a high thermal conductivity core material coated with silica; a composite material; an inorganic/organic hybrid surrounding material; a composite material coated with silica; a magnetic core material coated with an inorganic/organic hybrid surrounding material; or a high thermal conductivity core material coated with an inorganic/organic hybrid surrounding material. | 05-09-2013 |
20130105682 | TECHNIQUES FOR EFFICIENT FRAGMENTATION OF PEPTIDES | 05-02-2013 |
20130102478 | INTERNAL STANDARDS AND METHODS FOR USE IN QUANTITATIVELY MEASURING ANALYTES IN A SAMPLE - The invention provides methods for quantitatively analyzing a plurality of analytes in a sample. Also described are general and specific internal standards useful in such analysis. In particular embodiments, these standards are described as useful in liquid chromatography/mass spectroscopy systems, which are also described herein. Moreover, in certain embodiments, the quantification methods of the present invention are useful in increasing the precision and/or accuracy of multiple analyte quantification for analytes contained in a single sample mixture using known analyte derivatives simultaneously analyzed, and compared to the unknown analytes. | 04-25-2013 |
20130086058 | Synonym Groups - A computer-implemented method includes receiving a search query that includes a user specified search criteria directed toward one field of a plurality of fields associated with a synonym group, and performing a search of a database of a scientific data information system based on the search query. In addition to the user specified search criteria, the search includes all of other the fields associated with the defined synonym group. | 04-04-2013 |
20130080967 | Graphical User Interfaces For Scientific Data Information System - A method for assisting a user with interacting with laboratory data in a scientific data information system. The method includes displaying a graphical user interface having a sub-item navigation bar including a plurality of selectable tabs each associated with a corresponding one of a plurality of categories, and simultaneously displaying in the graphical user interface a main work area containing information about a selected one of the categories. The user can toggle from one category to another with a single input action. | 03-28-2013 |
20130080073 | TECHNIQUES FOR MASS SPECTROMETRY PEAK LIST COMPUTATION USING PARALLEL PROCESSING - Described are techniques for processing data. Sample analysis is performed generating scans of data. Each scan comprises a set of data elements each associating an ion intensity count with a plurality of dimensions including a retention time dimension and a mass to charge ratio dimension. The scans are analyzed to identify one or more ion peaks. Analyzing includes filtering a first plurality of the scans producing a first plurality of filtered output scans. The filtering including first filtering producing a first filtering output, wherein the first filtering includes executing a plurality of threads in parallel which apply a first filter to the first plurality of scans to produce the first filtering output. Each of the plurality of threads computes at least one filtered output point for at least one corresponding input point included in the plurality of scans. Analyzing includes detecting one or more peaks using the filtered output scans. | 03-28-2013 |
20130076029 | Gasket and Seal Apparatus - Embodiments of the present invention feature devices and methods for holding fluids at high pressures. One embodiment of the present invention features a fluid containing conveying device for receiving and discharging fluids. The device comprises a housing having a chamber for containing a fluid. The housing has a first gasket receiving surface for receiving a gasket. A gasket formed of a deformable material and having a first abutment surface and second abutment surface. The first abutment surface received on the first gasket receiving surface and the second abutment surface for receiving a chamber closing piece. The chamber closing piece having a second gasket receiving surface, the chamber closing piece for closing the chamber. At least one of the first gasket receiving surface of the housing and the second gasket receiving surface of the chamber closing piece having a retaining grove, having at least one edge. Compression means for compressing the gasket, deforming the material such that the gasket is pressed into retaining groove and gripped by the edge of the cavity to prevent gasket movement. | 03-28-2013 |
20130074196 | Signature Methods For Scientific Data Information Systems - A computer-implemented method that includes providing an authorized user with an option to define one more levels of a signature method, and providing the authorized user with an option to assign at least one user whose signature is required for each of the defined levels. | 03-21-2013 |
20130071867 | Method And Apparatus For Performing Mass Spectrometry - The invention feature devices and methods for preserving and processing samples comprising a fluid having deuterated compounds. The device of the present invention comprises a housing defining a first chamber and a second chamber. The first chamber is heated to an elevated temperature and receives the sample and performs a digestion process on the sample at the elevated temperature. The second chamber is cooled to a low temperature and receives the deuterated digested sample from the first chamber and performs one or more separation steps to isolate an analyte. The device of further comprises conduit means for containing and moving the sample into the first chamber to form a digested sample. The conduit means moves the digested sample from the first chamber to the second chamber to separate the sample to form at least one analyte. The analyte is maintained at the low temperature to preserve its deuterated form. | 03-21-2013 |
20130068043 | APPARATUS AND METHODS FOR PREPARATION AND ANALYSIS OF DRIED SAMPLES OF A BIOLOGICAL FLUID - Described is a device for collecting a fluid sample, such as a biological fluid sample. The device includes a planar collection substrate having an absorbent material. The planar collection substrate includes an impermeable region and a sample collection region. The impermeable region is impermeable to the fluid sample and is embedded in the planar collection substrate in a spatial pattern. The sample collection region is in an area excluded from the spatial pattern and has a shape and a size defined by the spatial pattern. The sample collection region is configured to receive a known volume of the fluid sample. In an alternative form, the device includes a sample collection element disposed in an impermeable planar holder and, in another alternative form, the device includes an absorbent material disposed inside an impermeable tube wall. | 03-21-2013 |
20130056631 | TECHNIQUES FOR AUTOMATED PARAMETER ADJUSTMENT USING ION SIGNAL INTENSITY FEEDBACK - Described are techniques for tuning parameters of a system. For one or more values of a distance, mass spectral analysis of a liquid analyte stream is performed. A corresponding ion signal intensity of a selected ion is obtained. The distance is measured from a first end of a first tube to a second end of a second tube which surrounds said first tube. Using a computing device, a current value of the distance is automatically adjusted in accordance with corresponding ion signal intensities obtained for the selected ion. Using a computing device, a desired value for the distance is automatically determined using the corresponding ion signal intensities. The desired value results in an ion signal intensity for the selected ion which is any of more than a threshold intensity and a maximum of ion signal intensities obtained by performing mass spectral analysis using different values for the distance. | 03-07-2013 |
20130056084 | Method And Apparatus For Sample Injection In Liquid Chromatography - The present invention provides a method and apparatus for substantially eliminating destructive transients of pressure or flow rate which can degrade the efficiency and useful lifetime of chromatography columns. The present invention enables a substantially constant flow of mobile phase liquid to be maintained through the chromatography system by eliminating the flow blockage interval associated with the actuation of sample injection valves. The present invention further provides a method to reduce the pressure and flow rate transients associated with pressurization of the sample loop contents when the sample loop is introduced to chromatography system delivery pressure. | 03-07-2013 |
20130054149 | SYSTEM AND METHOD FOR ABSOLUTE QUANTIFICATION OF PROTEINS USING LC/MS - Absolute quantitation of protein in a sample is provided by comparing a sum or average of the N highest ionization intensities observed for peptides of a particular protein along with a calibration standard. The calibration standard can be in the form of a table generated by prior protein peptide analysis performed using one or more pre-determined proteins. The comparison is used to determine a corresponding absolute quantity of protein based on the observed sum or average of ionization intensities. A simple conversion factor can be applied to the calibration standard value to determine the absolute quantity of protein in the sample. | 02-28-2013 |
20130053588 | METHODS, COMPOSITIONS, DEVICES, AND KITS FOR PERFORMING PHOSPHOLIPID SEPARATION - Methods, kits and devices for separating phospholipids and proteins from small molecules in biochemical samples can feature an apparatus having a wetting barrier, at least one fit and a separation media. For example, an apparatus can include at least one wall defining a chamber having an exit and an entrance; a wetting barrier disposed between the exit and entrance, so as to define a separation media space located between the wetting barrier and the exit and a sample receiving area located between the wetting barrier and the entrance; and a separation media disposed adjacent to the wetting barrier and having a specific affinity for phospholipids. The wetting barrier is adapted to (i) retain the liquid sample and a protein precipitating agent in the sample receiving area under a first force, thereby facilitating the formation of a protein precipitate and a processed sample, and (ii) flow the processed sample through the wetting barrier and separation media under a second force, wherein the second force is greater than the first force, thereby retaining the protein precipitate in the sample receiving area, retaining phospholipids in the separation media, and eluting small molecules. | 02-28-2013 |
20130052083 | COLUMN HEATER WITH ACTIVE PRE-HEATING - To heat a flowing liquid, an apparatus includes a heater block assembly having a heater block made of thermally conductive material. The heater block assembly has a tube inlet, a tube outlet, and a tube path between the tube inlet and tube outlet. Tubing extends through the tube path from the tube inlet to the tube outlet. The tubing is in thermal communication with the heater block. A heater cartridge, in thermal communication with the heater block, is configured to provide heat to the heater block for transfer to liquid flowing through the tubing between the tube inlet and the tube outlet of the heater block assembly. Circuitry is in electrical communication with the heater cartridge to control a temperature of the heater block by controlling operation of the heater cartridge. | 02-28-2013 |
20130043672 | Fluidic Coupler Assembly With Conical Ferrule - A fluidic coupler assembly ( | 02-21-2013 |
20130020483 | APPARATUS FOR PHOTOIONIZATION OF AN ANALYTE IN AN ELUENT OF A CHROMATOGRAPHY COLUMN - Described is an apparatus for photoionization of an analyte in an eluent of a chromatography column. The apparatus includes a laser to generate a beam of optical radiation, a mass spectrometer and one or more optical components to receive the beam and to generate a concentrated region of optical radiation near an orifice of the mass spectrometer so that an analyte in the vapor phase of the eluent is ionized. Alternatively, the apparatus includes a laser driven light source, a mass spectrometer and an optical imaging system. The laser driven light source includes an optical enclosure having an ionizable medium, an ignition source and a laser for pumping optical radiation into the ionizable medium. The optical imaging system generates an image of the pumped region of the ionized medium proximate to the orifice of the mass spectrometer to thereby photoionize an analyte in the vapor phase of the eluent. | 01-24-2013 |
20130019696 | Chromatography Apparatus And Methods Using Multiple Microfluidic Substrates - An apparatus for chemical separations includes a first substantially rigid microfluidic substrate defining a first fluidic port; a second substantially rigid microfluidic substrate defining a second fluidic port; and a coupler disposed between the first and second substrates, the coupler defining a fluidic path in fluidic alignment with the ports of the first and second substrates. The coupler includes a material that is deformable relative to a material of the first substrate and a material of the second substrate. The substrates are clamped together to compress the coupler between the substrates and form a fluid-tight seal. | 01-24-2013 |
20130019695 | APPARATUS FOR REDUCING VARIATION IN SAMPLE TEMPERATURES IN A LIQUID CHROMATOGRAPHY SYSTEM - Described is an apparatus for reducing a temperature variation of a liquid chromatography sample. A tray drive using at least one of linear or rotational motion moves a sample tray along a path inside a sample compartment of the liquid chromatography system. A control module coordinates the movement of the sample tray by the tray drive and the movement of a sample needle by a needle drive so that any sample in the sample tray can be injected into the mobile phase of the system during a sample load operation. The tray drive is also used to move the sample tray along the path during a temperature averaging period when no loading occurs. Differences in sample temperatures due to variations in the air temperature at different locations inside the sample compartment are reduced, leading to more accurate and repeatable chromatographic measurement results. | 01-24-2013 |
20130014569 | THERMAL-BASED FLOW SENSING APPARATUS AND METHOD FOR HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY - One aspect of the invention provides a flow sensing apparatus for sensing fluid flow in a nano-scale high-performance liquid chromatography apparatus. The flow sensing apparatus includes: a fluid channel that allows a fluid to flow in a first direction; a first infrared sensor arranged at a first position along the fluid channel such that it senses a temperature of the fluid; a second infrared sensor arranged at a second position along the fluid channel and separated from the first sensor by a predetermined distance along the fluid channel; and a heating element arranged between the first and second infrared sensors. The heating element is equally spaced from the first and second infrared sensors. | 01-17-2013 |
20130014567 | CHROMATOGRAPHY APPARATUS HAVING DIFFUSION-BONDED AND SURFACE-MODIFIED COMPONENTS - A microfluidic device for separating a sample by chromatography includes diffusion bonded metallic sheets joined together to create a hermetically sealed interface between each adjacent metallic sheet without the introduction of a secondary material. Enclosed within the diffusion bonded sheets is a separation channel accessible by at least one of an inlet or an outlet. The separation channel is packed with micrometer-sized particles serving as a stationary phase in a chromatographic separation. Wetted surfaces of the separation channel include a coating of an organic material at least one monolayer thick. | 01-17-2013 |
20130013225 | FLOW SENSORS AND FLOW SENSING METHODS WITH EXTENDED LINEAR RANGE - One aspect of the invention provides a flow sensing apparatus including: a fluid channel that allows a fluid to flow in a first direction; a first temperature sensor arranged at a first position along the fluid channel; a second temperature sensor arranged at a second position along the fluid channel and separated from the first sensor by a predetermined distance along the fluid channel; a heating element arranged between the first and second thermoelectric sensors, the heating element being substantially equally spaced from the first and second thermoelectric sensors; a heating element temperature sensor for sensing a temperature of the heating element; and a control device configured to maintain the heating element at a substantially uniform temperature. | 01-10-2013 |
20130004388 | SELF-SEALING SAMPLE COMPARTMENT FOR A LIQUID CHROMATOGRAPHY SYSTEM - Described is a self-sealing thermal enclosure. In various embodiments, the self-sealing thermal enclosure includes an enclosure having a wall with an opening. The enclosure is configured to surround a temperature-controlled environment. The self-sealing thermal enclosure also includes a porous seal disposed adjacent to the wall at the opening. The porous seal is compressible and is fabricated from an open cell foam material. When the porous seal has absorbed a fluid such as a condensate, the temperature-controlled environment is sealed from an ambient environment such that the flow of air into or out of the enclosure is substantially reduced or eliminated. | 01-03-2013 |
20120328486 | COMPOUND SAMPLE NEEDLE FOR LIQUID CHROMATOGRAPH - Described is a compound sample needle for a liquid chromatography system. In one embodiment, the compound sample needle includes a rigid needle having a coupling end with a face and a counterbore. The compound sample needle also includes a flexible tubing having an outer surface and a coupling end disposed in the counterbore of the rigid needle. The coupling end of the flexible tubing has a face adjacent to a base of the counterbore. The coupling ends of the rigid needle and flexible tubing are secured to each other by a weld formed along a circumference of the outer surface and the face of the rigid needle. A rigid sleeve protects the welded joint. The compound sample needle is suitable for injection into the high pressure mobile phase of ultra performance liquid chromatography systems. | 12-27-2012 |
20120305464 | ON-LINE SAMPLING FROM A PROCESS SOURCE - An online sample manager of a liquid chromatography system includes a fluidic tee having a first inlet port, a second inlet port, and an outlet port. A diluent pump moves diluent from a diluent source to the first inlet port of the fluidic tee. A valve has a fluidic intake port connected to a process source for acquiring a process sample therefrom. A pumping system moves the acquired process sample from the valve into the second inlet port of the fluidic tee where the process sample merges with the diluent arriving at the first inlet port to produce a diluted process sample that flows out from the outlet port of the fluidic tee. | 12-06-2012 |
20120304746 | THERMAL-BASED FLOW SENSING APPARATUSES AND METHODS FOR HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY - One aspect of the invention provides a flow sensing apparatus including: a fluid channel that allows a fluid to flow in a first direction; a first thermoelectric sensing element arranged at a first position along the fluid channel such that it senses a temperature of the fluid; a second thermoelectric sensing element arranged at a second position along the fluid channel and separated from the first sensing element by a predetermined distance along the fluid channel; and a heating element arranged between the first and second thermoelectric sensing elements, the heating element being equally spaced from the first and second thermoelectric sensing elements. | 12-06-2012 |
20120304745 | COORDINATION OF SOLVENT DELIVERY WITH PRE-INJECTION OPERATIONS IN A LIQUID CHROMATOGRAPH - A liquid chromatography system includes an autosampler that prepares a sample for introduction to a solvent stream and a solvent delivery system that delivers a solvent stream to the autosampler. Delivery of the solvent stream occurs in parallel with the autosampler's pre-injection operations. The autosampler starts pre-injection operations to make the sample ready for injection into the mixture stream, and the solvent delivery system starts delivery of a solvent stream to the autosampler. The start of the solvent stream delivery is coordinated with the start of the pre-injection operations such that the solvent stream arrives at the autosampler approximately coincident with when the autosampler completes the pre-injection operations, making the sample ready for injection. | 12-06-2012 |
20120303167 | COORDINATING INJECTOR-VALVE SWITCHING WITH PUMP-STROKE TIMING TO MITIGATE EFFECTS OF PRESSURE PULSES - A liquid chromatography system includes an autosampler with an injector valve by which a sample at low pressure within a sample loop is introduced into a high-pressure solvent mixture stream. A solvent delivery system includes a pump in fluidic communication with the injector valve of the autosampler to deliver the solvent mixture stream thereto. The solvent delivery system further comprises a processor that calculates a number of strokes of the pump needed to deliver the solvent mixture stream to the injector valve of the autosampler. The processor counts strokes of the pump during delivery of the solvent mixture stream. In response to a stroke count equaling the calculated number of strokes, the processor signals the autosampler to switch the injector valve to introduce the sample to the solvent mixture stream during a pump transfer period within which the pump performs pressure control to compensate for a drop in pressure. | 11-29-2012 |
20120287746 | MANIFOLD FOR SOLVENT MIXING IN LIQUID CHROMATOGRAPHY SYSTEMS - Low-pressure mixing of fluids includes a pumping system having a pump and a fluidic inlet port through which fluid is introduced to the pump. A fluid proportioning system is in fluidic communication with the fluidic inlet port of the pumping system to deliver thereto a fluid stream comprised of multiple different fluids. The fluid proportioning system includes a manifold having a plurality of inlet ports, an outlet port connected by tubing to the fluidic inlet port of the pumping system, and an outlet conduit providing an internal fluidic passageway to the outlet port. Each inlet port is fluidically coupled to a fluid source to receive one of the different fluids and to the outlet conduit to deliver thereto the received fluid for delivery out of the manifold through the outlet port to the fluidic inlet port of the pumping system. | 11-15-2012 |
20120286151 | Devices and Methods for Analyzing Surfaces - Embodiments of the present invention are directed to devices and methods for performing an analysis of a sample having a sample surface. The device and method feature a frame element affixed to a sample holder, jet element and a charged particle analyzer having an ion receiving orifice. The jet element directs a jet of gas towards the sample surface held by said sample holder focused on less than 2.0 mm | 11-15-2012 |
20120285872 | THERMAL CONTROL OF THERMAL CHAMBER IN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY SYSTEMS - A sample manager of a liquid chromatography system implements a thermal system that uses a dual-loop feedback control system to control temperature within a thermal chamber. The sample manager includes an external heatsink disposed externally to the thermal chamber, an internal heatsink disposed within the thermal chamber, and one or more thermoelectric devices thermally coupled to the external and internal heatsinks to transfer heat therebetween in response to a pulse-width modulated power signal. A first temperature sensor disposed within the thermal chamber continuously measures a chamber temperature. A second temperature sensor coupled to the internal heatsink within the thermal chamber continuously measures temperature at the internal heatsink. A feedback control system controls a duty cycle of the pulse-width modulated power signal in response to a target chamber temperature and real-time temperature measurements produced by the first and second temperature sensors. | 11-15-2012 |
20120285223 | METHOD FOR DETERMINING EQUIVALENT THERMAL CONDITIONS BETWEEN LIQUID CHROMATOGRAPHY SYSTEMS - Described is a method of transferring a chromatographic method between liquid chromatography (LC) systems. The method is based on a determination of an isoretention temperature at which two solutes co-elute. The method enables separations to be performed using different LC systems with reproducible and equivalent results. For example, the method allows for a chromatography method developed for HPLC to be more readily transferred to a UPLC system and for a chromatography method developed for a UPLC system to be more readily transferred to a HPLC system. The method addresses LC systems having column ovens of different design in which the internal column temperatures are not equal although the operating temperatures of the column ovens may be accurately controlled to equal values. The retention behavior and resolution of different LC systems is caused to be substantially the same so that equivalent separation results are obtained. | 11-15-2012 |
20120273404 | HIGH PURITY CHROMATOGRAPHIC MATERIALS COMPRISING AN IONIZABLE MODIFIER - The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are high purity chromatographic materials comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifier | 11-01-2012 |
20120262178 | Apparatus And Method For Coupled LC-NMR Analysis - A device for performing chromatographic separations and nuclear magnetic resonance analysis has trapping means for holding a separated sample and to form a held separated sample and placing said held separated sample in said nuclear magnetic resonance assembly. One preferred trapping means forms a held separated sample and a passed separated sample. The passed separated sample is discharged from the device. Preferred trapping means comprise a trapping column or a separated sample loop. | 10-18-2012 |
20120254872 | Content Items for Scientific Data Information Systems - Data information systems include memory for storing a first content item representing a predefined workflow. The first content item has a data format that complies with a unified data structure. The data information system further comprises a scientific instrument configured to acquire data in accordance with the predefined workflow, and a server in communication with the scientific instrument to obtain the data acquired by the scientific instrument. The server has a processor that executes program code to convert the acquired data into a second content item with a data format that complies with the unified data structure. The unified data structure can include an instance data element with fields that are specific to a type of content item, and a catalog data element having a copy of data extracted from the type-specific fields of the instance data element. | 10-04-2012 |
20120253684 | METHODS AND APPARATUS FOR PERFORMING RETENTION-TIME MATCHING - A method for matching a precursor ion with one or more related product ions includes providing input data sets obtained from sample injections, each of the data sets including a precursor ion and one or more product ions, normalizing the input data sets in accordance with a single retention time for the precursor ion, determining which product ions are within a predetermined retention time window with respect to the single retention time, and, if a product ion is within the predetermined retention time window for a specified number of the input data sets, determining that the product ion is related to the precursor having the single retention time. An apparatus for analyzing a sample includes a chromatography module, a mass-spectrometry module in communication with the chromatography module, and control unit in communication with the chromatography module and the mass-spectrometry module. | 10-04-2012 |
20120248033 | POROUS MATERIALS FOR SOLID PHASE EXTRACTION AND CHROMATOGRAPHY AND PROCESSES FOR PREPARATION AND USE THEREOF - Embodiments of the present invention are directed to porous materials for use in solid phase extractions and chromatography. The materials feature at least one hydrophobic component, at least one hydrophilic component and at least one ion-exchange functional group. The materials exhibit superior wetting and ion-exchange performance. | 10-04-2012 |
20120227470 | Chromatography Apparatus Having An Integrated Core - A chromatography apparatus includes a sample-delivery patterned substrate and a tube-based separation column directly connected to an outlet port of the patterned substrate. The patterned substrate includes an injector valve connected to the outlet port. | 09-13-2012 |
20120225444 | METHODS AND APPARATUS FOR FRACTIONATION-BASED CHEMICAL ANALYSES - A method for analyzing chemicals includes fractionating a complex sample into at least two sample portions that each include portions of two polypeptides though in different concentration ratios, digesting and performing LC/MS on each of the sample portions, and associating precursor ions observed via LC/MS with their corresponding polypeptide in response to LC/MS-provided intensity data. A set of precursor-ions that has substantially similar intensity ratios in both sample portions is determined to be associated with the same polypeptide. | 09-06-2012 |
20120216602 | Pressure Monitor Optimization Of Fluid Path Utilization - A device comprising a pressure monitor and a control means that receives a signal representing measured pressure at the pressure monitor and controls the controllable elements of a fluid system is utilized to monitor a fluid system for error conditions, to optimize operations and to diagnose the fluid system. By following a testing protocol that selectively enables parts of the system, the control means narrows the list of possible failing components. Comparing the measured pressure against normal pressures allows the device to identify error conditions. Determining the volume of fluid being transported and controlling the duration of the flow optimizes operation of the fluid system. | 08-30-2012 |
20120205532 | Techniques For Sample Analysis - Techniques are described for performing sample analysis. Liquid chromatographic separation of a sample is performed and an eluent is generated. Mass spectrometry on said eluent is performed to detect a compound where the compound may occur in trace amounts. The compound may have a concentration, for example, of approximately less than one part per trillion. | 08-16-2012 |
20120205304 | Self-Unlocking Fluid-Tight Connector - A connector is used to connect a conduit to a threaded orifice of a fluidic component, such as a component of an apparatus for chromatography. The connector includes a flexible fitting, a deformable fitting, and a compression fitting. The flexible fitting fixes the position of the conduit with respect to the threaded orifice. The deformable fitting provides a fluid-tight seal between the fluidic conduit and the threaded orifice. The compression fitting includes an external-thread portion for attachment to the orifice, to urge the flexible fitting toward an outer surface of the fluidic conduit, and to urge the deformable fitting toward both the outer surface and the orifice, a cam portion in mechanical communication with the flexible fitting via a through-hole in the external thread portion, and a graspable portion in rotatable communication with the cam portion. | 08-16-2012 |
20120189992 | Apparatus And Method For Establishing Standards For Visual Residue Limits - Embodiments of the present invention are directed to apparatus and methods for establishing standards for visual residue limits, training individuals to visually observe and recognize surfaces exceeding or complying with residue limits, and certifying individuals for such detection limits The invention features a kit comprising an assembly of coupons with predetermined loads. | 07-26-2012 |
20120171773 | Temperature Control Of Enrichment And Separation Columns In Chromatography - A method of analyzing samples includes loading a sufficient quantity of the sample onto a trap column to overload the trap column; heating an analytical column and the trap column to a greater temperature than the analytical column; and pumping a solvent, to the trap column, having a solvent composition profile that, in cooperation with a temperature differential, causes at least some of the components to elute sequentially from the trap column to the analytical column and focus on the analytical column prior to eluting from the analytical column; or optionally: loading a small-molecule sample onto a cooled portion of an analytical column; heating the analytical column; and pumping a solvent, to the heated analytical column, to elute the components from the analytical column. Chromatographic separation includes: a trap column; a separation column; a trap-column heater; a separation-column heater; a solvent pump unit; and a control unit can be used. | 07-05-2012 |
20120168311 | Systems And Methods For Coupling Molecule Separation Devices To Analytical Instruments - Described is a capillary that interfaces with an analysis system. The capillary comprises a non-conductive tubing and a conductive region proximate to an output end of the non-conductive tubing. The conductive region comprises a plurality of porous regions and non-porous regions positioned about a circumference of the non-conductive tubing. The porous regions separated from each other by the non-porous regions. Advantageously, the arrangement of porous regions provides mechanical stability about the conductive region. | 07-05-2012 |
20120160690 | Capillary Column Cartridge - Described is a capillary column cartridge. The cartridge can be used to perform separations according to various techniques such as capillary gas chromatography, capillary electrophoresis and capillary liquid chromatography. The cartridge includes a capillary column secured in a cartridge body. The capillary column includes an inlet port and an outlet port that, in some embodiments, are disposed on a planar surface of the body. When the body is engaged to a separation system module, the inlet port is aligned to receive a sample to be separated and the outlet port is aligned to provide the separated sample to the separation system module. The path of the capillary through the body has a non-planar path shape such as a coil shape. Consequently, longer column lengths can be accommodated, leading to an improvement in separation resolution. The body can include a material having a high thermal conductivity to achieve improved thermal performance. | 06-28-2012 |
20120153137 | METHODS FOR SEPARATING COMPOUNDS - Methods and systems for analyzing samples using multi-dimensional chromatography are disclosed. | 06-21-2012 |
20120132794 | Apparatus And Components Thereof For Liquid Chromatography - The invention provides chromatographic apparatus and components manufactured from a corrosion resistant alloy typically comprising iron, nickel, chromium and molybdenum. The apparatus and components exhibit superior biocompatibility and corrosion resistance, especially to mobile phases containing chlorides. They are especially useful in HPLC and HPLC, and are particularly useful in conjunction with chromatographic separation media comprising particles smaller than 2 μm diameter. | 05-31-2012 |
20120125843 | METHODS AND MATERIALS FOR PERFORMING HYDROPHOBIC INTERACTION CHROMATOGRAPHY - A method for performing hydrophobic interaction chromatography includes providing at least one wall defining a chamber having an inlet and an exit, and a stationary phase disposed within the chamber. The stationary phase comprises particles or monolith having a hydrophobic surface and a hydrophilic ligand. The method also includes loading a sample onto the stationary phase in the chamber and flowing the sample over the stationary phase. The sample is separated into one or more compositions by hydrophobic interaction between the stationary phase and the one or more compositions. | 05-24-2012 |
20120119128 | Rotary Shear Injector Valve With Coated Stator Surface - A valve includes a rotor and a stator. The rotor and the stator each have seal surfaces for contacting and sliding against one another during operation of the valve. Either or both of the rotor and the stator have a seal-surface coating formed by depositing an at least partially amorphous interlayer on a substrate and depositing a surface layer, including diamond-like carbon, the interlayer. The surface layer requires no mechanical polishing. | 05-17-2012 |
20120103075 | Automated Dilution For Liquid Chromatography - Described are a method and system for diluting a sample in a liquid chromatography system. A sample flowing at a first flow rate is combined for a predetermined time with a diluent flowing at a second flow rate to generate a volume of diluted sample. The diluted sample has a dilution ratio determined according to the two flow rates. A portion of the volume is loaded into a sample loop of an injection valve and subsequently injected into a mobile phase flowing to a chromatography column. The method eliminates the need to have a technician available to perform dilutions or to transfer the sample to a remote location for dilution. Advantageously, manufacturing or processing downtime to perform the dilution is not required and the amount of sample flowing to waste is reduced. | 05-03-2012 |
20120097026 | Piston Pump With Leak Diagnostic Port - Described are embodiments of a pump having a diagnostic port that enables users and support personnel to diagnose the source of a pump leak. The diagnostic port is monitored visually or with a pressure or liquid detection sensor. In some embodiments, fluid detected at the diagnostic port corresponds to a leak around a circumference defined at an outer sealing surface of a high pressure seal. In other embodiments, fluid detected at the diagnostic port corresponds to a leak around a plunger at the inside diameter of the high pressure seal. Multiple diagnostic ports can be provided to allow separate determinations of leaks about the different portions of the high pressure seal. The embodiments may eliminate the need to disassemble the pump to determine the source of leaks. Thus the effort required to diagnose and repair the pump can be substantially reduced, and fewer misdiagnosed components are ordered or replaced. | 04-26-2012 |
20120092664 | OPAQUE ADDITIVE TO BLOCK STRAY LIGHT IN TEFLON.RTM. AF LIGHT-GUIDING FLOWCELLS - The present invention is directed to the use of a light absorbing wall material to eliminate stray light paths in light-guiding structures, such as those used for HPLC absorbance detection. More specifically, the present invention relates to the use of carbon-doped Teflon® AF, or “black Teflon® AF,” for all or part of the walls of a light-guiding flowcell adapted for use in HPLC absorbance detection. | 04-19-2012 |
20120055860 | HYBRID INORGANIC/ORGANIC MATERIALS HAVING NOVEL SURFACE MODIFICATION; PROCESS FOR THE PREPARATION OF INORGANIC/ORGANIC HYBRID MATERIALS; AND USE OF SAID PARTICLES FOR CHROMATOGRAPHIC SEPARATIONS - The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The preparation of the inorganic/organic hybrid materials of the invention wherein a surrounding material is condensed on a porous hybrid core material will allow for families of different hybrid packing materials to be prepared from a single core hybrid material. Differences in hydrophobicity, ion-exchange capacity, surface charge or silanol activity of the surrounding material may be used for unique chromatographic separations of small molecules, carbohydrates, antibodies, whole proteins, peptides, and/or DNA. | 03-08-2012 |
20120040968 | DIAMINO HETEROCYCLIC CARBOXAMIDE COMPOUND - Provided is a compound useful as an inhibitor against the kinase activity of EML4-ALK fusion protein. | 02-16-2012 |
20120037724 | Electrospray Interface To A Microfluidic Substrate - An apparatus for chemical separations includes a microfluidic substrate having an outlet aperture for outputting an eluent of a sample, a spray unit having an inlet to receive the eluent and an outlet to emit a spray of the eluent, and a force-applying unit. The spray unit has a deformable portion defining the inlet and having an elastic modulus that is lower than an elastic modulus of the microfluidic substrate. The force-applying unit, such as a spring, is disposed to urge the deformable portion in contact with the substrate to form a substantially fluid-tight seal. | 02-16-2012 |
20120032628 | Intake Profile For Optimized Utilization Of Motor Characteristics - Systems and methods for operating a stepper motor of a pump at a desired low velocity include memory for storing information corresponding to an intake velocity profile. The intake velocity profile represents an optimized acceleration curve for operating the stepper motor over a range of motor velocities during an intake cycle. A processor of a system controller dynamically accesses the memory during the intake cycle to acquire the information representing the intake velocity profile and issues a series of pulses to the stepper motor based on this information. In response to the pulses, the stepper motor accelerates in accordance with the optimized acceleration curve represented by the intake velocity profile. The optimized acceleration curve is based on the available torque of the stepper motor across a range of motor velocities and enables the motor to operate with greater torque utilization and less margin than traditional linear acceleration profiles. | 02-09-2012 |
20120024399 | Electrospray Interface To A Microfluidic Substrate - A liquid-chromatography module includes a microfluidic cartridge housing a microfluidic substrate with a channel for transporting fluid. The microfluidic substrate has fluidic apertures through which fluid is supplied to the channel. One side of the cartridge has nozzle openings, each aligning with a fluidic aperture in the microfluidic substrate and receiving a fluidic nozzle. A clamping assembly has a plunger that is movable into a clamped position and an end housing that defines a chamber. One wall of the end housing has a fluidic block with fluidic nozzles extending into the chamber. A second wall has a slot through which the cartridge enters the chamber. When moved into the clamped position while the cartridge is in the chamber, the plunger urges the cartridge against the fluidic block such that each fluidic nozzle enters one nozzle opening and establishes fluidic communication with one fluidic aperture in the microfluidic substrate. | 02-02-2012 |
20110315633 | Rotating Valve - Described is a rotary valve including a stator element and a rotor element. The stator element defines a first inlet passage, a second inlet passage, and a first outlet passage, respectively, having a first inlet port, second inlet port and first outlet port in a stator face. The rotor element has a rotor face in contact with the stator face and has a first channel and a second channel formed therein. The rotor element is rotatably movable about a rotational axis to at least a first position and a second position. In the first position, the first inlet port is fluidly coupled to the first outlet passage by the first channel and the second inlet port is fluidly coupled to the first outlet passage by the second channel. In the second position, the first inlet port is fluidly coupled to the first outlet passage by the first and second channels. | 12-29-2011 |
20110290994 | Methods And Apparatus For Performing Gas And Liquid Mass Spectrometry - Embodiments of the present invention feature devices and methods for performing gas chromatography and liquid chromatography with mass spectrometry. The device and method feature a removable housing which housing contains the gas samples when the mass spectrometry receives gas samples. The device fits an atmospheric pressure ionization housing and upon removal, the atmospheric pressure ionization housing receives liquid samples. | 12-01-2011 |
20110278214 | Liquid-Chromatography Conduit Assemblies Having High-Pressure Seals - A method for making a liquid-chromatography apparatus includes inserting two inner conduits into an intermediate tube, inserting the intermediate tube into an outer tube, forming a proximal seal between the intermediate tube and at least one of the inner conduits, and forming a distal seal between the intermediate tube and at least one of the inner conduits. A liquid-chromatography apparatus includes an outer tube, an intermediate tube disposed in the outer tube, two inner conduits disposed in the intermediate tube, a proximal seal between the intermediate tube and at least one of the inner conduits, and a distal seal between the intermediate tube and at least one of the inner conduits. | 11-17-2011 |
20110272855 | Techniques For Patterning Valve Components - Described are techniques for fabricating one or more parts of a valve used in a liquid chromatography system. At least one of a rotor and a stator are provided. The rotor is included in the valve and has a first surface facing a stator. The stator is included in the valve and has a second surface facing the rotor. A pattern is formed in at least one of the first surface and the second surface. Forming the pattern includes compressing the at least one surface by applying pressure thereto causing displacement of material to form at least one groove. | 11-10-2011 |
20110271755 | Apparatus And Methods For The Measurement Of Mass Related Parameters - Embodiments of the present invention are directed to resonating detection devices in which the sample flows through a conduit having a section in which the conduit is grouped or concentrated with the point of maximum movement. | 11-10-2011 |
20110269241 | Methods And Kits For The Determining The Presence Or Absence Of Cyanobacteria Toxins - Embodiments of the present invention are directed to kits and methods for the detection of toxins produced by cyanobacteria. The methods and kits feature sample preparation steps with weak cationic and anionic exchange resins and small particle analytical columns operating at 4,000 to 15,000 psi. | 11-03-2011 |
20110266434 | Stacked-Electrode Peptide-Fragmentation Device - A chemical processing apparatus includes a fragmentation device that includes a linear set of stacked electrodes and a voltage control module that forms DC potential wells of opposite polarity for mutual confinement of opposite polarity ions. A method of protein analysis includes confining positive peptide ions and negatively charged reagent anions in, respectively, first and second DC potential wells in a fragmentation device, mixing the ions, in the fragmentation device, and analyzing ion fragments formed in the mixture. | 11-03-2011 |
20110266433 | Apparatus And Methods For Gas Chromatography - Mass Spectrometry - The invention provides improved apparatus for, and methods of, ionizing analyte molecules in a flow of gas, typically at or around atmospheric pressure. The invention may be used to facilitate mass spectrometric analysis of analytes comprised in the effluent form a gas chromatograph. Ionization may be effected by atmospheric pressure chemical ionization or by atmospheric pressure photo ionization. | 11-03-2011 |
20110263033 | Apparatus And Methods For Performing Photoreactions And Analytical Methods And Devices To Detect Photo-Reacting Compounds - Embodiments of the present invention are directed to devices and methods for performing photoreactions of photo-reacting compounds in solution. The invention features a vessel and a light source. The vessel has at least one wall defining a chamber. The chamber, for performing photoreactions, has a chamber volume, a first window, an inlet and an outlet. The inlet and outlet are in fluid communication with a source of photo-reacting compounds in solution. The first window is transparent to light transmission and is placed in optical communication with a light source to receive photons. The chamber is for receiving a solution over time to define a dwell time. The solution potentially has one or more photo-reactive compounds having a concentration. The device further comprises a light source, in optical communication with the first window, for emitting photons which photons are received by the first window and transmitted into the chamber. | 10-27-2011 |
20110263029 | Methods And Kits For The Determining The Presence Or Absence Of Ergot Alkaloids - The present invention is directed to methods and kits for the determining the presence or absence of ergot alkaloids in sample which use chromatographic instrumentation and columns operating at a pressure of 4,000 to 15,000 psi and columns with 1-3 micron particle size to produce results by mass spectroscopy in approximately four minutes. | 10-27-2011 |
20110261642 | Valve Switch Modulation For Reducing Errors Due Oscillations Of The Inlet Fluid Of A Pump System - Described is a method of reducing liquid composition errors in a low-pressure mixing pump system. Packets representing the switching intervals of each component of the desired fluid mixture are provided to an intake of the mixing pump system. For each packet, a switching time associated with at least one of the components in the packet is modulated. Modulated switching times are based on time offsets that are specifically selected according to the undesirable frequency characteristic of an intake response of the mixing pump system. The average of the volumes contributed by the packets thus modulated is equal to a component volume that achieves a desired proportion of the component in the output flow of the mixing pump system. Modulated switching times enable the reduction or elimination of composition error in the output flow of the mixing pump system. | 10-27-2011 |