Sigma Aldrich Company Patent applications |
Patent application number | Title | Published |
20120017290 | Genome editing of a Rosa locus using zinc-finger nucleases - Disclosed herein are methods and compositions for genome editing of a | 01-19-2012 |
20100218264 | Genome editing in rats using zinc-finger nucleases - Disclosed herein are methods and compositions for genome editing of one or more loci in a rat, using fusion proteins comprising a zinc-finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins. | 08-26-2010 |
20100009451 | COMPOSITIONS AND METHODS FOR SPECIFICALLY SILENCING A TARGET NUCLEIC ACID - The present invention provides methods modified oligonucleotides and methods of using the modified oligonucleotides for silencing nucleic acids, wherein the nonspecific effects of nucleic acid silencing are reduced. | 01-14-2010 |
20090240044 | PROCESS FOR THE REDUCTION OF ENDOTOXINS IN A PLASMID PREPARATION USING A CARBOHYDRATE NON-IONIC DETERGENT WITH SILICA CHROMATOGRAPHY - The present invention provides methods for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography. | 09-24-2009 |
20090131279 | NUCLEIC ACID FLUORESCENT STAINS - The present invention provides fluorescent dye compounds and methods of using the compounds for the staining of nucleic acids. In particular, the dye compounds comprise heterocyclic molecules with hydroxy alkyl and aromatic substituents, and the dye compounds form highly fluorescent complexes upon nucleic acid binding. | 05-21-2009 |
20090107948 | BOTTLE AND CAP DEVICE - A cap and bottle device is provided that includes a cap, a septum, and a container that individually and collectively improves the capacity of the assembly to securely contain substances. The cap includes internal threads that mate external threads of the container containing the substance. The cap further includes flexible teeth for engaging rigid lugs that are molded into the finish on the neck of the container. The septum sealably fits between the cap and within an opening of the container to provide barrier between the contained substance and the surrounding environment. | 04-30-2009 |
20090099040 | DEGENERATE OLIGONUCLEOTIDES AND THEIR USES - The present invention provides a plurality of oligonucleotides comprising a semi-random sequence, wherein the semi-random sequence comprises degenerate nucleotides that are substantially non-complementary. Also provided are methods for using the plurality of oligonucleotides to amplify a population of target nucleic acids. | 04-16-2009 |
20090061424 | UNIVERSAL LIGATION ARRAY FOR ANALYZING GENE EXPRESSION OR GENOMIC VARIATIONS - The present invention provides an array system comprising a plurality of immobilized oligonucleotides comprising artificial sequences and a plurality of complementary ligation templates, as well as methods and kits for using the array system to analyze populations of nucleic acids. In particular, target nucleic acids are ligated to the immobilized oligonucleotides on the array in the presence of the complementary ligation templates. | 03-05-2009 |
20080299023 | CONSOLIDATED VACUUM SUBLIMATION MODULE - A consolidated vacuum sublimation module (VSM) can comprise an upper body portion, a middle body portion, a lower body portion, a bushing, and a seal. A vapor director cartridge can be loaded with a crude material and manually inserted into a cartridge-holding member of the lower body portion. Assembly of the middle and upper body portions form a structural unit that can compressively and sealably connect to the lower body portion. During sublimation, heat is effectively transferred to the lower body portion that contains the crude material. A substantial fraction of the middle body portion is conically tapered about a central longitudinal axis to promote desublimation. The middle body portion is a unitary structure that consolidates valving, cooling, and desublimation functions. Following completion of an operational cycle, the vacuum sublimation module is inspected for sublimate presence, then valve-isolated, suitably cooled, detached, opened to a preferred gaseous atmosphere, disassembled, and manipulated for solids recovery. | 12-04-2008 |
20080213906 | COMPOSITIONS AND METHODS FOR COMBINING PROTEIN PRECIPITATION AND SOLID PHASE EXTRACTION - A composition, method and device for the preparation of biological samples for subsequent LC-MS analysis using a combined and concurrent protein precipitation and solid phase extraction (SPE) process is described. Through an integrated combination of protein precipitation, filtration, and SPE using a novel zirconia-coated chromatographic media, interfering compounds, such as proteins and phosphate-containing compounds, are eliminated from the biological samples, affording a higher degree of analyte response during LC-MS analysis. | 09-04-2008 |