Patent application title: Methods and Compositions of Nucleic Acid Ligands for Detection of Clinical Analytes Related to Human Health
Inventors:
John Bruno (Boerne, TX, US)
Assignees:
OTC BIOTECHNOLOGIES, LLC
IPC8 Class: AC12N15115FI
USPC Class:
536 231
Class name: Nitrogen containing n-glycosides, polymers thereof, metal derivatives (e.g., nucleic acids, oligonucleotides, etc.) dna or rna fragments or modified forms thereof (e.g., genes, etc.)
Publication date: 2014-10-02
Patent application number: 20140296500
Abstract:
Specific DNA sequences for binding various clinically relevant analytes
from the human body are described. Each of these sequences or their
linear, two- and three-dimensional linked sequences can function in
varying assay and sensor formats with varying degrees of success. Linkage
of the whole or partial DNA sequences (putative binding sites) can be
used to enhance specificity and affinity towards complex targets, thereby
improving assay selectivity and sensitivity in many instances. In
addition, a FRET-based quantitative method is described for normalizing
analyte data by assessing urine creatinine and urea levels. Finally, a
method is described for removing creatinine or urea by size-exclusion
chromatography prior to a FRET-based aptamer assay to avoid the
denaturing effects of these compounds.Claims:
1. A DNA ligand sequence consisting of SEQ ID NO.: 4.
2. A DNA ligand sequence consisting of SEQ ID NO.: 434.
3. A DNA ligand sequence consisting of SEQ ID No.: 462.
Description:
CLAIM OF PRIORITY TO PRIOR APPLICATION
[0001] This application claims the benefit of the filing date of U.S. Provisional Application Ser. No. 61/402,491, filed on Aug. 31, 2010; U.S. Provisional Application Ser. No. 61/463,020, filed on Feb. 10, 2011; and U.S. Non-Provisional application Ser. No. 13/199,484, filed on Aug. 31, 2011, each of which is entitled "Methods and Compositions of Nucleic Acid Ligands for Detection of Clinical Analytes Related to Human Health", the entire disclosures of which are hereby incorporated by reference into the present disclosure, to include the Sequence Listing(s) previously submitted with the subject applications.
BACKGROUND OF THE INVENTION
[0002] 1. Field of the Invention
[0003] The present invention pertains to the field of aptamer- and nucleic acid ligand (DNA and RNA ligand)-based diagnostics. More specifically, the application relates to single-stranded deoxyribonucleic acid ("DNA") and ribonucleic acid ("RNA") ligand sequences, whether individual or linked together to form longer multiple binding site "receptors," that specifically target and bind to clinically relevant analytes on one or more binding sites or "epitopes" from humans such as cardiovascular biomarkers, bone metabolism markers, glucose, natural or recombinant human growth hormone ("hGH" or somatotropin) and vitamins. The invention includes general DNA ligand or aptamer-based detection and quantitation of these analytes in body fluids such as blood plasma, serum, sputum or saliva, interstitial, synovial, or cerebrospinal fluid aspirates, mucus, and urine or solid biopsy samples.
[0004] 2. Background Information
[0005] These individual or linked DNA ligand (aptamer) sequences represent valuable target analyte-responsive components of diagnostic devices or biosensors. A "biosensor" is defined as any device that employs a biologically-derived molecule as the sensing component and transduces a target analyte binding event into a detectable physical signal (including, but not limited to, changes in light intensity, absorbance, transmittance, refraction (Surface Plasmon Resonance or "SPR"), wavelength, color, agglutination of cells or particles, fluorescence intensity, fluorescence lifetime, fluorescence polarization or anisotropy, fluorescence correlation spectroscopy ("FCS"), fluorescence or Forster resonance energy transfer ("FRET"; nonradiative dipole-dipole coupling of fluorophores or fluorophores and quenchers), upconverting phosphor, two-photon interaction phenomena, Raman spectroscopy or surface-enhanced Raman spectroscopy ("SERS"), electrical conduction, electrical resistance or other electrical properties, mass, photon or radioactive particle emissions, etc).
[0006] Once bonded with the target, these DNA ligand sequences can be used to qualitatively determine the presence of analyte, as well as to quantify or semi-quantify the target analyte amount in a sample using a broad variety of assay types and diagnostic or sensor platforms including, but not limited to, affinity-based lateral flow test strips, membrane blotting, SPR, surface acoustic waveguides ("SAW" devices), magnetic bead ("MB")-based capture, plastic-adherent sandwich assays ("PASA"), chemiluminescence ("CL"), electrochemiluminescence ("ECL"), radioisotopic, fluorescence intensity, including quantum dot ("QD") or other fluorescent nanoparticle ("FNP") of dye-based, fluorescence lifetime, and fluorescence polarization ("FP") assays or enzyme-linked ("ELISA-like") microplate assays. ELISA-like assays refer to microwell or microplate assays similar to traditional Enzyme-Linked Immunosorbent Assays ("ELISA") in which an aptamer or nucleic acid ligand is substituted for the antibody or receptor component or components, but the other components such as peroxidase or alkaline phosphatase enzymes and color-producing substrates remain the same.
[0007] In addition, these DNA ligand sequences are valuable in competitive displacement assays which are not solely dependent on high affinity (strong attractive forces between a receptor and its ligand) or high avidity (high tensile or physical strength of receptor-ligand bonds) to produce sensitive detection (sub-nanoMolar or sub-nanogram levels), because the equilibrium constant (generally Ka=106 to 108 to enable competition) must allow reasonable displacement of previously bound target materials to detect a change at or below nanogram or nanoMolar levels.
[0008] In a competitive displacement assay, labeled DNA ligand plus labeled analyte complexes compete with unlabeled analyte to bind with the labeled DNA. After allowing the labeled and unlabeled analytes to come to equilibrium with the labeled DNA, the unlabeled target analyte may be quantitatively assayed by fluorescence intensity or other methods. Such assays would include competitive displacement FRET assays or DNA ligand "beacon" FRET assays. In a competitive displacement FRET assay the fluorophore ("F") and quencher ("Q") are placed in a putative binding loop or pocket so as to reside within the Forster distance of 60-85 Angstroms to enable quenching.
[0009] In an aptamer beacon assay, the F and Q labels are placed on the 5' and 3' ends and binding of the target analyte to the beacon opens the beacon beyond the Forster quenching distance so that F is no longer quenched and emits light generally in proportion to the amount of target analyte introduced into the liquid system. Each of these types of aptamer assays and detection platforms has different applications in either central medical laboratories or in point-of-care ("POC") sensor devices for use in emergency rooms, intensive care units, cardiac care units, or physician's offices and clinics.
SUMMARY OF THE INVENTION
[0010] The DNA ligand sequences listed herein (Table 9) were derived by iterative cycles of affinity-based selection, washing, heated elution, and polymerase chain reaction ("PCR") amplification of bound DNA ligands from a randomized library using immobilized target analytes for affinity selection and PCR amplification followed by cloning and Sanger dideoxynucleotide DNA sequencing.
[0011] Sanger dideoxynucleotide sequencing refers to DNA chain termination due to a lack of a 3' hydroxyl (--OH) group to link incoming bases to during DNA synthesis followed by automated fluorescence reading of the DNA sequence from an electrophoresis gel containing all of the terminated DNA fragments. DNA sequencing may be accomplished by PCR doped with dideoxynucleotides lacking hydroxyl groups at the 2' and 3' sugar ring positions and thereby disallowing chain formation. PCR refers to the enzymatic amplification or copying of DNA molecules with a thermo-stable DNA polymerase such as Thermus aquaticus polymerase (Taq) with known "primer" regions or short oligonucleotides of known sequence that can hybridize to a longer target DNA sequence to enable priming of the chain reaction (exponential doubling of the DNA target copy number with each round of amplification).
[0012] A randomized library can be chemically synthesized by linking together the four deoxynucleotide triphosphate bases (adenine; A, cytosine; C, guanine; G, and thymine; T) in equal amounts (25% each), so that a combinatorial oligonucleotide arises with sequence diversity equal to 4 raised to the nth power (4n) where n is the desired length of the randomized region in bases. In other words, if position 1 in an oligonucleotide is allowed to consist of A, C, G, or T (diversity=4) by equal availability of all 4 bases and these 4 possibilities are multiplied by each base linking to 4 more possible bases at position 2, then this process yields 16 possible 2-base oligonucleotides (i.e., AA, AC, AG, AT, CA, CC, CG, CT, GA, GC, GG, GT, TA, TC, TG, TT) and so on for the entire chosen length (n) of the randomized region. This combinatorial progression displays immense diversity as a function of oligonucleotide chain length. For example, an oligonucleotide decamer of 10 base length could be expected to contain 4n=410 or 1,048,576 unique DNA sequences from which to chose or select one or more DNA sequences that bind a given immobilized target analyte with the strongest affinities.
[0013] The randomized oligonucleotide or DNA is designed to be flanked on either side by short primer regions of known and fixed sequences to enable PCR amplification (exponential copying) of the rare sequences that are selected from the random library by binding to the target after the non-binding members of the random library are washed away (not selected).
[0014] Additional assays, such as ELISA-like plate assays or fluorescence (e.g., intensity and FRET) assays, may be used to screen or verify the value of particular DNA and RNA ligands or aptamer sequences for detection of a given target analyte in a given assay format or type of biosensor. Some of the sequences operate (bind and transduce the binding signal) more effectively in affinity-based (ELISA-like or fluorescence intensity) assays, while other DNA ligand sequences against the same targets function better in lower affinity competitive or other assays, thereby leading to more sensitive detection with lower limits of detection (sub-nanoMolar or sub-nanogram) and less cross-reactivity or more specificity for the target analyte. "Specificity" or "selectivity" means the ability to selectively exclude molecules similar in structure to the true target analyte that may interfere with the assay and give false indications of detection. All of the listed DNA ligand nucleotide sequences have potential applications in some type of assay format, because they have survived at least 5 rounds of affinity-based selection and enrichment (by PCR amplification), although some of the sequences will undoubtedly perform better in certain assay formats or configurations (in tubes, square cuvettes, membranes, or on biochips) than others.
[0015] Combinations of the DNA ligands, whether in whole or in part (i.e., their binding sites of approximately 5-10 or more nucleotides or bases), could be linked together in a linear or 2-dimensional ("2-D") or 3-dimensional ("3-D") fashion similar to dendrimers as shown in FIG. 1B to bind multiple epitopes or binding sites on a complex target analyte (Ag or antigen) such as a virus or whole prokaryotic (bacterial) or eukaryotic (animal, fungal or plant) cell surface having numerous spatially separated epitopes of different types. The advantage of linking aptamers or their shorter binding pockets, loops or binding sites is that the nascent linear, 2-D, or 3-D aptamer construct will likely have improved affinity or "avidity" (tensile binding strength) making it more difficult to remove or dissociate from the target antigen. Higher affinity and avidity generally lead to greater assay sensitivity and specificity which are typically desirable traits in many assays.
[0016] The linked aptamer complex will be likely to gain specificity as well since the probability of binding to multiple epitopes with any degree of success is multiplicative. Thus, the ability to bind to epitopes A, B and C equals the product of the probability of binding to A with high affinity times the probability of binding to B with high affinity times the probability of binding to C with high affinity. The product of those three fractional probabilities is clearly much less than the probability of binding to only A, B, or C independently in the absence of binding the other two or any combination of the two epitopes therein in the absence of binding the third. Hence by linking two or more aptamers or their binding regions with or without DNA or other "spacer" regions, the selectivity or specificity of aptamers or DNA ligands can be increased.
[0017] This approach to binding site linkage emulates the nature of antibodies which demonstrates linkage of their "hypervariable" ("HV") regions on the antigen combining sites of the immunoglobulin ("Ig") light and heavy chains. In the HV regions, the variability of the 20 amino acid types is quite high and essentially represents a selection of one combination from a large combinatorial library in the protein realm (similar to down selection of a few candidate aptamers from a large diverse starting library). The trait of HV region linkage contributes to Ig affinity, avidity and specificity. Similarly, linking aptamers or aptamer binding sites for various epitopes in one, two or three dimensions will enhance larger aptamer or DNA ligand construct affinity, avidity, and selectivity or specificity as illustrated in FIGS. 1A and 1B.
[0018] The present invention provides specific DNA sequence information for nucleic acid ligands (aptamers) or their linked constructs selected from randomized pools to bind clinically important proteins, peptides, hormones, sugars, vitamins, post-translational N-acetylglucosamine (NAG or O-GlcNAc) modifications of key proteins, etc. in a variety of assay formats and sensor or diagnostic platforms for assessment of human health status. While all of the candidate sequences have been shown to bind their cognate targets, some are shown to function more effectively in affinity-based assays versus fluorescence resonance energy transfer (FRET) or other assay formats that rely more on physical parameters other than affinity such as fluorophore-quencher proximity (i.e., the Forster distance). Therefore, all of the sequences are potentially valuable for simple qualitative detection or quantitative assays, but some may function better in terms of sensitive and specific detection than others in particular assay formats.
BRIEF DESCRIPTION OF THE DRAWINGS
[0019] FIG. 1A illustrates an antibody, revealing the multiple hypervariable antigen combining of binding sites on both the heavy and light chains.
[0020] FIG. 1B illustrates the concept of linking aptamers or their binding sites in a linear fashion (although 2-D and 3-D linkages are also possible) to mimic the linkage of multiple hypervariable (HV) regions in antigen combining sites of antibody chains to enhance affinity, avidity, and specificity against complex target antigens (Ag) containing two or more distinct epitopes.
[0021] FIG. 2 represents secondary stem-loop structures of DNA ligands or aptamers (SEQ ID NOs 3 and 4).
[0022] FIGS. 3A-3F show how the aptamer beacons from FIG. 2 behaved as a function of decreasing CTx peptide concentration.
[0023] FIG. 4A illustrates specificity of the 15-base CTx 2R-2h aptamer beacon for the full-length 26-amino acid (AA) version of the CTx peptide.
[0024] FIG. 4B shows the same samples evaluated by a handheld fluorometer that only reports fluorescence peak height, but confirms the level of specificity.
[0025] FIG. 5 shows the relative linearity and sensitivity of the 15-base CTx 2R-2h aptamer beacon assay.
[0026] FIG. 6A illustrates the ability to normalize urine concentration readings with simple aptamer beacon fluorescence readings.
[0027] FIG. 6B shows a quantitative estimation of creatinine and urea levels in urine or serum using the aptamer beacon denaturation approach.
[0028] FIG. 7A illustrates that the 2,942 dalton (26-amino acid) CTx bone peptide can be extracted from urine to avoid the denaturing effects of creatinine and urea on the CTx 2R-2h aptamer beacon assay by use of a desalting size-exclusion polyacrylamide bead column.
[0029] FIG. 7B shows that CTx peptide extracted from human urine by means of a desalting column can still be detected to a level of at least 122 ng/ml by the CTx 2R-2h aptamer beacon.
[0030] FIG. 8 illustrates the secondary stem-loop structures of several aptamers which dominated a sequenced pool of vitamin D3 aptamers (abbreviated VD3 and from the SEQ ID NOs 429-526).
[0031] FIGS. 9A-9D illustrate the FRET responses of loops A, B, and C for the aptamers shown in FIG. 8.
[0032] FIGS. 10A-10C reveal that the VD3 Loop C beacon appears to react equally well with (A) 1-hydroxy-vitamin D2, (B) 1-hydroxy-vitamin D3 and (C) 25-hydroxy-vitamin D3.
[0033] FIG. 11 shows an assessment of the VD3 Loop C beacon.
[0034] FIGS. 12A and 12B summarize assessment of the specificity or cross-reactivity of the VD3 Loop C aptamer beacon versus a variety of potential analytes or interfering species.
[0035] FIGS. 13A and 13B show fluorometric spectra from two separate trials of a competitive displacement FRET assay.
[0036] FIG. 14 shows the same samples from FIG. 13 assessed for fluorescence peak height.
[0037] FIGS. 15A-15D show four examples of aptamers from the pool of aptamers that bind recombinant human growth hormone.
[0038] FIGS. 16A-16D show four more examples of aptamers from the pool of aptamers that bind recombinant human growth hormone.
[0039] FIG. 17A shows an experimental matrix screening scheme for capture aptamer-conjugated magnetic microbeads.
[0040] FIG. 17B shows mean bar heights of three separate measurements.
[0041] FIGS. 18A and 18B give ECL line plots for assay combinations 18 and 40 from the matrix in FIG. 17A.
[0042] FIG. 19A shows an experimental matrix screening scheme for capture aptamer-conjugated magnetic microbeads.
[0043] FIG. 19B shows mean bar heights of three separate measurements.
[0044] FIG. 20A shows the linear ECL response for the sandwich assay of FIG. 19.
[0045] FIG. 20B shows cross-reactivity of the combination number 4 BNP assay versus other peptide or protein analytes that might be found at the 100 ng/ml level in human blood.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
[0046] There is no single preferred embodiment for use of the DNA aptamer ligand sequences or linked aptamer constructs identified herein. Rather, the sequences are useful to varying extents in a variety of assay formats and sensors or diagnostic devices chosen from at least the following list: lateral flow test strips, Surface Enhanced Raman (SERS), Surface Plasmon Resonance (SPR), Surface Acoustic or Transverse Wave (SAW or STW) detection, electrical, electrochemical, colorimetric absorbance, agglutination, ELISA-like or enzyme-linked microplate assays, magnetic bead-based capture assays, ECL or other chemiluminescence assays, radioisotopic assays and a variety of fluorescence assays including, but not limited to, fluorescence intensity, fluorescence lifetime, fluorescence polarization (FP) and Fluorescence or Forster Resonance Energy Transfer (FRET) assays (both beacon and competitive FRET (Bruno et al., 2010, 2011) in round tubes, square or flat cuvettes, or immobilized on magnetic beads, other types of microbeads, or flat surfaces such as nitrocellulose, nylon, or other membranes or on glass or plastic DNA microarrays or "biochips."
[0047] While there may appear to be considerable variability among sequences that bind the same clinical analyte targets, "epitopes" and their cognate aptamer binding sites are usually quite small (e.g., 5-10 bases) and a single target may contain numerous individual binding sites or epitopes for multiple aptamer binding. In addition, however, there is often a common or consensus sequence (designated herein by slashes between clone numbers in Table 9, e.g. --aptamer clones CTx 2, 13, 19, 20, 25, 32F or R series are identical and only listed once as SEQ ID NO 3 and 4) or common segments of 5-10 or more nucleotides in a row within otherwise different aptamer sequences that can bind a specific target epitope that may dominate the other binding sites by being more physically accessible or having stronger electrostatic, hydrogen bonding, or other attractive forces (summation of van der Waals or other weak forces). Variations in nucleotide sequences around these consensus segments or common binding sequence segments may serve to modulate the binding segment's affinity or specificity or may have no effect at all. These properties must be determined by empirical comparisons.
DNA Ligand (Aptamer) Selection and Generation
[0048] General methods for developing DNA ligands or aptamers to the immobilized proteins, peptides, or small molecules (defined as less than 1,000 daltons) are as follows. The protein, peptide or an amino-derivative of the small molecule (such as glucosamine in the case of D-glucose or dextrose) is then added to 2×109 tosyl-coated magnetic beads (MBs; e.g., Dynal brand from Invitrogen Corp. Carlsbad, Calif., 2. 8 micron size) for 2 hours at 37° C. The tosyl group is a "leaving" group that allows the formation of a very stable covalent bond between primary amine groups in the target protein, peptide or amino-derivatized small molecule and therefore immobilizes the target on the surfaces of the MBs so that they can be used to probe the randomized DNA library for DNA ligands. Target molecule-conjugated MBs (or target-MBs) are collected for 2 minutes in a magnetic collection device using an external magnet and the supernate is carefully withdrawn with a pipette tip. Target-MBs are then resuspended by vortexing briefly in 1× Binding Buffer (1×BB; 0.5M NaCl, 10 mM Tris-HCl, and 1 mM MgCl2, pH 7.5-7.6,) and washed by agitation for 5 minutes. MBs are collected and washed three times in this manner and then resuspended in 1 ml of 1×BB.
[0049] MB-based DNA ligand or aptamer development is then performed using a template library sequence such as: 5'-ATCCGTCACACCTGCTCT-N36-TGGTGTTGGCTCCCGTAT-3', where N36 represents the randomized 36-base region of the DNA library (maximal sequence diversity=436 in theory). Primer sequences are: 5'-ATACGGGAGCCAACACCA-3' (designated forward) and 5'-ATCCGTCACACCTGCTCT-3' (designated reverse) to prime the template and nascent strands for PCR, respectively. The random library is reconstituted in 500 μl of sterile nuclease-free water and heated to 95° C. for 5 minutes to ensure that the DNA library is completely single-stranded and linear. The hot DNA library solution is added to 100 μl of target-MBs (2×108 beads) with 600 μl of sterile 2× Binding Buffer (2×BB). The DNA library and target-MB suspension (1.2 ml) is mixed at room temperature (RT, approximately 25° C.) for 1 hour. Target-MBs with any bound DNA (round 1 aptamers) are magnetically collected. The DNA-target-MB complexes are washed three times in 400 μl of sterile 1×BB. Following the third wash, the DNA-target-MB pellet (about 75 μl) is used in a PCR reaction to amplify the bound DNA as follows. The MB pellet is split into 15 μl aliquots and added to five pre-made PCR tubes which contain most of the nonperishable ingredients of a PCR reaction beneath a wax seal. A total of 3 μl of 1:10 primer mix (10% forward primer plus 10% reverse primer) in nuclease-free deionized water or ˜20 nanomoles of each primer per ml plus 1 μl (5 U) of Taq DNA polymerase and 5 μl of 2 mM MgCl2 are added to each of the five tubes. PCR reactions are supplemented with 0.5 μl of E. coli single-strand binding protein (SSBP, Stratagene Inc., La Jolla, Calif.) to inhibit high molecular weight concatamer (end to end aggregates of the DNA ligands) formation. PCR is carried out as follows: an initial 95° C. phase for 5 minutes, followed by 20 cycles of 1 minute at 95° C., 1 minute at 53° C., and 1 minute at 72° C. followed by a 72° C. completion stage for 7 minute, and refrigeration at 4° C. This constitutes the first of multiple rounds of MB-aptamer development. Iterations of the MB-aptamer development process are repeated until the desired affinity or assay sensitivity and specificity are achieved. Typically, 5-10 rounds of the MB-aptamer development process are required to achieve low ng/ml detection of target analytes. To begin the second round and all subsequent rounds, 4 complete tubes of the 5 original PCR tubes are heated to 95° C. for 5 minutes to release bound DNA from the target-MBs. The fifth tube is always retained and refrigerated as a back-up for that round of the aptamer generation process. All available DNA (25 μl per tube) is siphoned out of the hot tubes without removing the target-MBs before the tubes cool significantly and the DNA is pooled. The 100 μl of hot DNA is added to 100 μl of fresh target-MBs in 200 μl of 2×BB and allowed to mix for 1 hr at RT. Thereafter, the selection and amplification process are repeated for 3-8 more rounds with checking for 72 bp aptamer PCR products by ethidium bromide-stained 2% agarose electrophoresis after each round. Following the last round of aptamer development, aptamers are cloned into chemically competent E. coli using a cloning kit from Lucigen Corp. (Middleton, Wis.) and clones are sent to Sequetech, Inc. (Mountain View, Calif.) for DNA sequencing.
Screening of Aptamers for Highest Affinity, Lowest Cross-Reactivity and to Determine Lower Limit of Detection by Target Titration in ELISA-Like Plate Assay ("ELASA")
[0050] To evaluate, screen, and rank aptamers based on affinity against clinically relevant targets, an enzyme-linked plate assay is conducted by first immobilizing 100 μl of 1:10 diluted target (about 0.1 mg of total protein, peptide or small molecule) in 0.1M NaHCO3 (pH 8.5) overnight at 4° C. in a covered polystyrene 96-well plate. The plate is decanted and washed three times in 250 μl of 1×BB. Each of the different 5'-biotinylated aptamers raised against the target is dissolved in 1×BB at 1.00 nmoles to 4.50 nmoles per 100 microliters and applied to their corresponding plate wells for 1 hour at room temperature (RT; ˜25° C.) with gentle mixing on an orbital shaker. The plate is decanted and washed three times in 250 μl of 1×BB for at least 5 minutes per wash with gentle mixing. One hundred μl of a 1:2,000 dilution of streptavidin-peroxidase from a 5 mg/ml stock solution in 1×BB is added per well for 30 minutes at RT with gentle mixing. The plate is decanted and washed three times with 250 μl of 1×BB per well as before. One hundred μl of ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) substrate with stabilized hydrogen peroxide (Kirkegaard Perry Laboratories, Inc., Gaithersburg, Md.) is added per well for 10 minute at RT. Finally absorbance is quantified using a microplate reader with 405 nm optical filter.
[0051] As Tables 1-8 illustrate for several cardiovascular biomarker targets (Brain Natriuretic Peptide; BNP, D-Dimer; DD, Creatine Kinase-MB types I and II; MBI and MB2, Interleukin-18; IL18, and Troponin-T; Tpn all at (1 μg/ml) the initial ELASA screening is useful for ranking the relative affinity of aptamers for their respective targets by simple ranking of absorbance values at 405 nm from highest to lowest. Each of the Tables (1-8) illustrates general consistency between ELASA trials as well (i.e., the highest affinity aptamers consistently rank among the highest absorbance values between ELASA trials or plates 1-4 maximally).
TABLE-US-00001 TABLE 1 DNA Ligand ELASA Rankings for Brain Natriuretic Peptide (BNP) Well Aptamer A 405 nm Plate 1 A12 BNP - 6R 2.766 C1 BNP - 14bF 2.283 A3 BNP - 2F 2.276 B1 BNP - 7F 2.227 E3 BNP - 25cf 2.215 A1 BNP - 1F 2.184 B4 BNP - 8R 2.176 A8 BNP - 4/9R 2.163 C3 BNP - 15aF 2.162 D4 BNP - 21bR 2.149 Plate 2 A12 BNP - 6R 2.171 A3 BNP - 2F 2.110 C1 BNP - 14bF 2.089 A8 BNP - 4/9R 1.989 A1 BNP - 1F 1.987 B9 BNP - 13F 1.986 A9 BNP - 5/11/15b/19/25bF 1.977 C2 BNP - 14bR 1.971 D9 BNP - 23bF 1.961 B4 BNP - 8R 1.953 Plate 3 A12 BNP - 6R 2.285 D5 BNP - 22F 2.272 E3 BNP - 25cF 2.240 D4 BNP - 21bR 2.212 C1 BNP - 14bF 2.206 B4 BNP - 8R 2.197 A9 BNP - 5/11/15b/19/25bF 2.196 A3 BNP - 2F 2.176 C3 BNP - 15aF 2.165 B5 BNP - 10F (70) 2.157
TABLE-US-00002 TABLE 2 DNA Ligand ELASA Rankings for D-Dimer (DD) Well Aptamer A 405 nm Plate 1 A10 DD - 5R 1.888 B10 DD - 12R 1.427 C7 DD - 17F (71) 1.261 B8 DD - 11R (71) 1.122 B12 DD - 13R (71) 1.088 B1 DD - 7F (71) 1.027 B7 DD - 11F (71) 1.021 B2 DD - 7R (71) 1.017 A12 DD - 6R (71) 0.989 C12 DD - 20R (71) 0.952 Plate 2 A10 DD - 5R 1.809 A4 DD - 2R (71) 1.620 C7 DD - 17F (71) 1.237 B10 DD - 12R 1.213 A12 DD - 6R (71) 1.005 B7 DD - 11F (71) 1.003 B8 DD - 11R (71) 0.986 B1 DD - 7F (71) 0.964 C11 DD - 20F (71) 0.932 B6 DD - 10R (71) 0.930 Plate 3 B1 DD - 7F (71) 0.803 C1 DD - 14F (71) 0.652 A10 DD - 5R 0.576 B4 DD - 9R (71) 0.545 B2 DD - 7R (71) 0.510 C4 DD - 15R 0.508 B5 DD - 10F (71) 0.418 C12 DD - 20R (71) 0.404 B10 DD - 12R 0.386 A5 DD - 3F (71) 0.370 Plate 4 C1 DD - 14F (71) 0.816 B1 DD - 7F (71) 0.806 A10 DD - 5R 0.749 B10 DD - 12R 0.732 B4 DD - 9R (71) 0.645 C3 DD - 15F 0.635 B5 DD - 10F (71) 0.563 B2 DD - 7R (71) 0.535 A5 DD - 3F (71) 0.526 C4 DD - 15R 0.512
TABLE-US-00003 TABLE 3 DNA Ligand ELASA Rankings for Creatine Kinase-MB Type I (MBI) Well Aptamer A 405 nm Plate 1 F1 MBI - 20F 2.917 D2 MBI - 1/4/5/8/9/17R (71) 2.725 D6 MBI - 3R (70) 2.668 E10 MBI - 16R 2.665 E1 MBI - 11F 2.658 E2 MBI - 11R 2.651 E11 MBI - 19F (69) 2.589 E7 MBI - 15F 2.578 D1 MBI - 1/4/5/8/9/17F (71) 2.556 D4 MBI - 2R (71) 2.522 Plate 2 F1 MBI - 20F 2.692 D2 MBI - 1/4/5/8/9/17R (71) 2.680 D6 MBI - 3R (70) 2.647 E1 MBI - 11F 2.612 E10 MBI - 16R 2.612 D3 MBI - 2F (71) 2.511 E2 MBI - 11R 2.499 D1 MBI - 1/4/5/8/9/17F (71) 2.497 F2 MBI - 20R 2.424 D4 MBI - 2R (71) 2.419 Plate 3 F1 MBI - 20F 1.863 D2 MBI - 1/4/5/8/9/17R (71) 1.858 D3 MBI - 2F (71) 1.808 E3 MBI - 12F (70) 1.753 E2 MBI - 11R 1.716 E5 MBI - 14/18F 1.692 D4 MBI - 2R (71) 1.663 E4 MBI - 12R (70) 1.642 E1 MBI - 11F 1.640 F2 MBI - 20R 1.619 Plate 4 F1 MBI - 20F 1.948 D3 MBI - 2F (71) 1.823 D6 MBI - 3R (70) 1.746 D2 MBI - 1/4/5/8/9/17R (71) 1.744 F2 MBI - 20R 1.742 D1 MBI - 1/4/5/8/9/17F (71) 1.733 E2 MBI - 11R 1.659 E3 MBI - 12F (70) 1.647 D5 MBI - 3F (70) 1.629 D4 MBI - 2R (71) 1.607
TABLE-US-00004 TABLE 4 DNA Ligand ELASA Rankings for Creatine Kinase-MB Type II (MBII) Well Aptamer A 405 nm Plate 1 G1 MBII - 6/8F 2.206 G12 MBII - 12R (71) 2.191 F12 MBII - 5R 2.173 H6 MBII - 14R 1.989 G4 MBII - 7R 1.910 H7 MBII - 15F (71) 1.838 G11 MBII - 12F (71) 1.827 H5 MBII - 14F 1.794 F10 MBII - 4R (71) 1.751 G9 MBII - 11F 1.732 Plate 2 G1 MBII - 6/8F 2.120 G4 MBII - 7R 1.931 G12 MBII - 12R (71) 1.850 H5 MBII - 14F 1.688 F7 MBII - 3F (71) 1.682 G5 MBII - 9F 1.675 G11 MBII - 12F (71) 1.640 H7 MBII - 15F (71) 1.611 F12 MBII - 5R 1.588 F9 MBII - 4F (71) 1.586
TABLE-US-00005 TABLE 5 DNA Ligand ELASA Rankings for Interleukin-18 (IL18) Aptamer Well A 405 nm Plate 1 IL18 - 29F D1 1.433 IL18 - 10F B1 1.428 IL18 - 5F A7 1.393 IL18 - 22R C4 1.373 IL18 - 21R C2 1.367 IL18 - 21F C1 1.270 IL18 - 29R D2 1.219 IL18 - 3F A3 1.217 IL18 - 15R B6 1.209 IL18 - 22F C3 1.204 Plate 2 IL18 - 22R C4 1.738 IL18 - 21R C2 1.697 IL18 - 10F B1 1.673 IL18 - 29F D1 1.655 IL18 - 5F A7 1.596 IL18 - 22F C3 1.594 IL18 - 26F (71) C7 1.553 IL18 - 21F C1 1.534 IL18 - 29R D2 1.528 IL18 - 30F (71) D3 1.525 Plate 3 IL18 - 29F D1 1.288 IL18 - 21R C2 1.202 IL18 - 22R C4 0.993 IL18 - 10F B1 0.917 IL18 - 26F (71) C7 0.886 IL18 - 22F C3 0.855 IL18 - 2F A1 0.844 IL18 - 29R D2 0.795 IL18 - 5F A7 0.791 IL18 - 4R A6 0.785 Plate 4 IL18 - 21R C2 1.060 IL18 - 26F (71) C7 0.841 IL18 - 4R A6 0.835 IL18 - 22F C3 0.677 IL18 - 22R C4 0.662 IL18 - 27/31F (70) C9 0.613 IL18 - 33R D8 0.608 IL18 - 27/31R (70) C10 0.601 IL18 - 5F A7 0.589 IL18 - 29F D1 0.550
TABLE-US-00006 TABLE 6 DNA Ligand ELASA Rankings for Troponin-T (Tpn) Aptamer Well A 405 nm Plate 1 Tpn - 20F (71) H3 2.325 Tpn - 12R (71) G2 2.070 Tpn - 20R (71) H4 2.053 Tpn - 7F (71) F3 2.030 Tpn - 18F H1 1.986 Tpn - 18R H2 1.971 Tpn - 6R (71) F2 1.962 Tpn - 1R (71) E2 1.949 Tpn - 15F (71) G7 1.898 Tpn - 1F (71) E1 1.871 Plate 2 Tpn - 20F (71) H3 2.642 Tpn - 20R (71) H4 2.479 Tpn - 1F (71) E1 2.374 Tpn - 12R (71) G2 2.357 Tpn - 6R (71) F2 2.342 Tpn - 18R H2 2.304 Tpn - 7F (71) F3 2.298 Tpn - 12F (71) G1 2.252 Tpn - 1R (71) E2 2.249 Tpn - 6F (71) F1 2.195 Plate 3 Tpn - 2OF (71) H3 2.482 Tpn - 2OR (71) H4 2.249 Tpn - 16R (71) G10 1.854 Tpn - 16F (71) G9 1.811 Tpn - 6R (71) F2 1.656 Tpn - 7F (71) F3 1.599 Tpn - 15R (71) G8 1.535 Tpn - 9R F8 1.517 Tpn - 1F (71) E1 1.487 Tpn - 4bR (71) E10 1.477 Plate 4 Tpn - 20F (71) H3 2.507 Tpn - 20R (71) H4 2.259 Tpn - 16F (71) G9 1.525 Tpn - 6R (71) F2 1.514 Tpn - 16R (71) G10 1.507 Tpn - 18F H1 1.396 Tpn - 11F (71) F11 1.386 Tpn - 4bR (71) E10 1.350 Tpn - 1F (71) E1 1.318 Tpn - 7F (71) F3 1.264
TABLE-US-00007 TABLE 7 DNA Ligand ELASA Rankings for C-Reactive Protein (CRP) Rank Aptamer Abs 405 nm 1 CRP-5R 2.332 2 CRP-10R 2.27 3 CRP-2R 2.210 4 CRP-11R 2.173 5 CRP-17R 2.165 6 CRP-23F 2.139 7 CRP-10F 2.100 8 CRP-2F 2.072 9 CRP-9R 2.066 10 CRP-9F 2.055
TABLE-US-00008 TABLE 8 DNA Ligand ELASA Rankings for Myoglobin (Myo) Rank Aptamer Abs 405 nm 1 Myo-3R/4R 2.395 2 Myo-22F 2.367 3 Myo-15F 2.344 4 Myo-3F/6F 2.337 5 Myo-5R 2.327 6 Myo-24F 2.294 7 Myo- 2.272 18R/27F 8 Myo-15R 2.269 9 Myo-28F 2.264 10 Myo-8F 2.250
Aptamer Beacons and Competitive FRET-Aptamer Assays
[0052] Once key aptamers have been identified by the commonality of their sequences or their secondary stem-loop structures, the assay developer decides upon secondary structure loops (potential binding pockets) to label with a fluorophore (F) or quencher (Q) as illustrated by dotted lines that define the borders or limits of potential binding loops in FIGS. 2 and 8. Secondary stem-loop structures are easily generated by Gibbs free energy minimization with common software such as M-fold and Vienna RNA (using DNA parameters) that is freely available for public use. The researcher or inventor simply enters the DNA sequence and selects a temperature and secondary stem-loop structures like those shown in FIG. 2 are generated. At this point, one can empirically assess candidate aptamer "beacon" potential in FRET analyte titration experiments such as those shown in FIGS. 3A-3F. The suspected short aptamer beacon loop is re-synthesized independent of the original larger parent aptamer sequence with a fluorophore (F) such as TYE 665 attached to the 5' end and a matched quencher (Q) such as Iowa Black attached to the 3' end (or vice versa), purified by HPLC or other form of chromatography and assessed for fluorescence output or intensity as a function of different levels of the target analyte (e.g., FIGS. 3A-3F and FIGS. 9A-9D). The greatest separation of fluorescence peak values or spectral emissions (e.g., FIGS. 3A-3F and FIGS. 9A-9D) is used to define the optimal beacon since separation of fluorescence values as a function of analyte concentration is essentially the definition of a good quality fluorescence assay.
[0053] Alternatively, one may label the suspected binding loops internally and place an F or a Q somewhere in the mid-section of the suspected loop other than the 3' or 5' end (i.e., intrachain FRET). Attachment of F or Q is usually accomplished via succinimide linkage of F- or Q-succinimides added to amino-modified aptamers at specifically chosen locations in the binding pockets. Primary amine linker moieties such as the "UniLink®" can be added internally at the time of chemical synthesis of aptamers. Typically 1 mg or more of an aptamer sequence is synthesized with a primary amine linker moiety (UniLink®) located at the approximate center of each loop structure (suspected binding pockets). Each of these internally amine-labeled aptamers is then labeled with 100 μl (0.1 mg) of F-succinimide (or alternatively Q-succinimide) for 2 hours in a 37° C. incubator, followed by purification through a 1×BB-equilibrated PD-10 (Sephadex G-25; GE Healthcare) column. In the meantime, an equal molar amount of primary amine-modified target molecule is labeled with 0.1 mg of spectrally matched Q-succinimide (to accept photons from F) at 37° C. for 2 hours and then washed three times by centrifugation at 14,000 rpm for 10 minutes per wash and resuspension in 1 ml of 1×BB. "Spectrally matched" means that most of the wavelengths of light emitted by F can be effectively absorbed by Q because its absorbance spectrum largely overlaps the emission spectrum of F. Naturally, if the aptamer is labeled with a Q-succinimide in the alternate form of the assay, the amine-modified target must be labeled with an appropriately matched F-succinimide to be quenched when bound to the Q-labeled aptamer. Pooled one ml fractions of purified F-labeled DNA aptamers are mixed with an equimolar amount of Q-labeled-amino-target analyte (or vice versa in the alternate embodiment) for 30 minutes at RT with mixing in 1×BB or phosphate buffered saline (PBS, 0.1M phosphates in 8.5 g/L sodium chloride at pH 7.2 to 7.4) and then purified through an appropriate size-exclusion chromatography column (according to molecular weight of the combined F-aptamer plus Q-target complex) to produce a purified "competitive FRET complex" consisting of F-aptamer conjugate bound to Q-labeled target. This competitive FRET complex can later be competed against unlabeled cognate analyte concentrations to increase the fluorescent light output of the liquid assay system and quantify the unlabeled analyte concentration.
[0054] Generally, the aptamer beacons or competitive FRET-aptamer complexes are then diluted to a final concentration of 1-5 μg/ml in 1×BB and equally dispensed to polystyrene or methacrylate cuvettes in which 1 ml of unlabeled target at various concentrations in 1×BB, PBS or diluted blood, plasma, serum, saliva, aspirate or urine has been added already. Cuvettes are gently mixed for 15 to 20 minutes at RT prior to reading their fluorescence in the homogeneous beacon or competitive-displacement FRET assay formats using a spectrofluorometer having gratings to vary the excitation wavelength and emission scanning ability or a stationary, handheld or otherwise portable fluorometer having a more restricted or fixed excitation and emission optical filter set with a range of wavelengths for excitation and emission.
Aptamer or Aptamer Binding Site Linkage in One or More Dimensions
[0055] The linkage of binding sites is beneficial in terms of enhancing receptor affinity, avidity (tensile binding strength), and selectivity versus complex targets with two or more distinct epitopes. This linkage can be sequential and linear (one-dimensional as in antibody heavy and light chain linkage of HV regions, FIG. 1A) or could be expanded into two or three dimensions much like DNA dendrimers or other more complex structures known to those skilled in the art. Linear linkage by chemical synthesis is quite facile, if one knows that aptamer DNA sequences or shorter (approximately 5-10 base) binding site sequences to be linked. One can simply design one long sequence to incorporate the desired aptamers or binding sites with repetitive poly-adenine (A), poly-cytosine (C), poly-guanine (G), poly-thymine (T), poly-uridine (U), or other intervening sequences that are unlikely to bind the target epitopes. The length of the composite aptamer construct will be limited by current chemical synthesis technology to about 200 bases. However, cellular biosynthesis or enzymatic synthesis by polymerase chain reaction (PCR) or asymmetric PCR (producing predominately single-stranded ss-DNA from a template) would not be so limited and should produce aptamer constructs up to 2,000 bases before the Taq polymerase falls off the template. The 2 kilobase Taq polymerase limit is the basis for the well-known RAPD (Random Amplification of Polymorphic DNA) method of DNA or genetic "fingerprint" analyses in which primers greater than 2 kilobases apart fail to produce a PCR product or amplicon, because Taq becomes disengaged from the template DNA before traveling 2,000 bases. In this way, lengthy aptamer constructs of less than 2 kilobases could be made from complementary DNA templates that would enable binding of different epitopes that are distal on the surface of relatively large objects such as viruses and whole bacterial or eukaryotic cells. Again, poly-A, C, G, T, or U or other linker nucleotide segments could be designed into the cDNA template to produce the resultant nascent strand to ligate aptamers or aptamer binding sites together into one contiguous linear chain with intervening linkers.
[0056] For 2-D or 3-D linked aptamer structures a variety of linker chemistries are available, but the preferred embodiment is probably addition of a UniLink® primary amine group somewhere in the mid-section of a larger multi-aptamer construct followed by covalent linkage of two or more such multi-aptamer constructs by means of bifunctional linkers such as low levels (≦1%) of glutaraldehyde, carbodiimides, sulfo-EGS, sulfo-SMCC or other such bifunctional linkers familiar to those skilled in conjugate chemistry. This strategy would result in a larger flower-like 2-D or 3-D structure consisting of two or more-lengthy multi-aptamer structures.
[0057] Referring to the figures, FIG. 1A illustrates the general structure of an IgG antibody showing the linkage of hypervariable (HV) amino acid regions used for actual binding to target epitopes on complex antigens. Linear linkage of HV binding sites adds affinity, avidity and specificity the antibody binding to complex targets. Likewise in FIG. 1B, aptamers or their shorter (5-10 base) binding sites can be linked during chemical or biochemical (enzymatic) synthesis to enhance aptamer binding affinity, avidity or specificity for improved assay sensitivity and selectivity.
[0058] FIG. 2 is a diagram of secondary structures for two "finalists" (SEQ ID NOs 3 and 4) from the pool of 24 unique candidate aptamers (SEQ ID NOs. 1-24) that bind human Type I bone collagen C-telopeptide (CTx) indicative of bone loss when found in the urine. These 2 aptamer sequences (forward and reverse or F and R-primed) dominated the aptamer pool (12 of 38 total clones=31.6%) and were therefore considered prime candidates to investigate for possible binding pockets (secondary loop structures). These potential binding pockets are shown as loops cut off from the rest of the aptamer by dotted lines and designated according to the 12 hour clock as 2 O'clock (or second hour or 2 h), 6 O'clock (6 h) and 10 O'clock (10 h). The overall 5' and 3' ends as well as a numbering system from bases 1 (at the 5' end) to base 72 (at the 3' end) are also indicated. A variation on the CTx 2R-2 h loop or beacon consisting of 13 and 15 bases (13b or 15b) is also indicated. The 2 O'clock or second hour (2 h) aptamer was subsequently synthesized to produce an aptamer beacon (3' end labeled with a quencher molecule and the 5' end labeled with a fluorophore) as demarcated by the dotted lines which is capable of detecting CTx peptide to low nanogram per ml levels.
[0059] FIGS. 3A-3F show how each of the aptamer beacons derived and defined from FIG. 2 behaved as a function of decreasing CTx peptide concentration (serial two-fold dilutions beginning with 100 micrograms per ml and ending with zero CTx peptide) in 1× binding buffer (1×BB; 0.5 M NaCl, 10 mM Tris-HCl, pH 7.5-7.6, 1 mM MgCl2). In the figures "h" means hour or O'clock so that 2 h refers to the 2 O'clock loop from FIG. 2, while F means forward-primed and R means reverse-primed by reference to FIG. 2 as well. FIGS. 3A-3F demonstrate that there is in fact a significant difference in the ability of each candidate loop or candidate beacon from FIG. 2 to bind and detect a 26-amino acid (AA; full-length) CTx peptide in 1×BB. The figures show that the 15-base CTx 2R-2h loop (5'-GGTGGTGTTGGCTCC-3') acts as a superior aptamer beacon for detection of CTx peptide, because it gives the greatest fluorescence spread as a function of CTx peptide concentration. The CTx 2R-2h loop was optimal for FRET-based detection because it gives the greatest spread or separation for various two-fold serial dilutions of the 26-AA CTx peptide beginning at 100 μg/ml. The CTx 2F-10 h loop was also noteworthy, but could not discriminate many of the different CTx concentrations. The candidate loops or beacons from this experiment were 5' labeled with TYE665 fluorophore and 3' labeled with Iowa Black RQ quencher and HPLC-purified prior to use at Integrated DNA Technologies, Inc. (Coralville, Iowa). Excitation on a spectrofluorometer was at 645 nm with 5 nm slits and a PMT (photomultiplier tube) setting of 900V. A slightly shorter 13-base version of the CTx-2R-2h beacon ((5'-GTGGTGTTGGCTC-3') has also been shown to work almost as well as the 15-base version, but has slightly higher background fluorescence. Excitation in all cases was at 645±5 nm to maximally excite TYE 665 dye (fluorophore) on the 5' end of each potential aptamer beacon with a photomultiplier tube (PMT) detector setting of 1,000 Volts.
[0060] FIGS. 4A and B demonstrate relative specificity of the CTx 2R-2h aptamer beacon. FIG. 4A illustrates specificity or preference of the beacon for binding to the full-length 26-AA version of the CTx peptide based on its more intense fluorescence with the full-length CTx peptide, but not with a shorter 8-amino acid segment of the same peptide using a spectrofluorometer. Since the 8-AA version of the CTx peptide (one letter amino acid sequence: EKAHDGGR) represents a subset of the larger 26-AA peptide (amino acid sequence: SAGFDFSFLPQPPQEKAHDGGRYYRA), this observation indicates where the aptamer does not bind on the larger CTx peptide and narrows down the possible binding sites to SAGFDFSFLPQPPQ or YYRA). The aptamer beacon does unexpectedly cross-react with and bind an epitope on bovine serum albumin (BSA) and since the 607 amino acid BSA protein shares its longest region of commonality with the CTx peptide at the amino acid sequence SFL or serine-phenylalanine-leucine, this may be the actual binding site of the CTx 2R-2h aptamer beacon to CTx bone peptide, although other sites cannot be ruled out completely. Again, intact bovine proteins and epitopes are not expected in human clinical samples, making the CTx 2R-2h aptamer beacon specific for its target bone peptide.
[0061] FIG. 4B shows the same samples FIG. 4A evaluated by a handheld fluorometer that only reports fluorescence peak height, but confirms the level of specificity seen in the spectra above. The beacon was again 5' labeled with TYE665 fluorophore and 3' labeled with Iowa Black quencher and HPLC-purified prior to use in assays. Excitation on the spectrofluorometer was at 645 nm with 5 nm slits and a PMT (photomultiplier tube) setting of 900V. Handheld fluorometer values were obtained with an optically modified (red light emitting diode with peak excitation at 650 nm with a 660-720 nm emission filter) Quantifluor® device from Promega Corp. Other abbreviations used in FIGS. 3A-3F are: NTx; N-terminal telopeptide of human Type I bone collagen, HP; helical peptide of human bone, BSA; bovine serum albumin, DPD; deoxypyridinoline, and Pyd; pyridinoline.
[0062] FIG. 5 compares the 15-base CTx 2R-2h aptamer beacon assay's titration versus different levels of the full-length CTx peptide (26 amino acids) in 1× binding buffer for 30 minutes at room temperature as assessed by the handheld Quantifluor® from 0 to 32 ng/ml of CTx peptide with a limit of detection (LOD) of 1 ng/ml with a photodiode standard value setting of 999.0. It shows the relative linearity and sensitivity of the 15-base CTx 2R-2h aptamer beacon assay.
[0063] FIGS. 6A and 6B illustrate the linear normalization response of three different aptamer beacons raised against creatinine from the SEQ ID NOs 239-294 family to the denaturing effects of creatinine across the physiologic range found in human urine. The non-specific linear response of aptamer beacons in general to the denaturing (linearizing) effects of creatinine and urea across the physiologic range of these substances found in human urine suggest that addition of almost any aptamer beacon to urine could be used to estimate creatinine and urea levels in urine and normalize the values for other analytes in urine. It is a common practice in clinical diagnostics in which the creatinine or urea level of urine is used as a divisor for the amount of other analytes detected in urine to normalize readings between different patients. In essence, the creatinine or urea levels indicate how concentrated the urine is and that information can be used to adjust or normalize (divide by) the creatinine level to place the levels of other analytes in proper perspective (i.e., high analyte levels in a dehydrated individual may be misleading). Quantitative estimation of creatinine and urea levels in urine or serum using the aptamer beacon denaturation approach is simple (one step bind and detect), rapid (within 10-15 minutes) and facile as compared to the more complicated multi-component alkaline picrate colorimetric and time-consuming Jaffe method (i.e., results obtained greater than 35 minutes after reagent and sample preparation).
[0064] FIGS. 7A and 7B illustrate that the 2,942 dalton (26 amino acid) CTx peptide can be extracted from urine to avoid the denaturing effects of creatinine and urea on the CTx 2R-2h aptamer beacon assay (demonstrated in FIG. 5) by use of a desalting size-exclusion polyacrylamide column. FIG. 7A shows fractions collected from a desalting column (with a 1,800 dalton molecular weight cut off) and run in an 8% polyacrylamide-sodium dodecyl sulfate (SDS) electrophoresis gel which was run against molecular weight protein standards (last lane) and Coomassie blue stained to reveal the location of the 2.9 kilodalton CTx peptide which emerged in the void volume fractions 4 and 5 (boxed). The far right lane of this gel shows molecular weight protein standards beginning at 5 kD. FIG. 7B shows that CTx peptide extracted from human urine by means of a desalting column can still be sensitively detected to a level of at least 122 ng/ml by the CTx 2R-2h aptamer beacon.
[0065] FIG. 8 illustrates the secondary stem-loop structures of several candidate aptamers (SEQ ID NOs 433 and 434) which dominated the sequenced pool of vitamin D3 aptamers (abbreviated VD3 and from the SEQ ID NOs 429-526). Again, the lines and capital letters demarcate the loops which were considered further as binding pockets or for aptamer beacons to detect vitamin D and its isoforms or congeners.
[0066] FIGS. 9A-9D illustrate the FRET responses of loops A, B, and C for the VD3 aptamers shown in FIG. 8 as well as handheld (Quantifluor®-P) fluorometer peak fluorescence values as a function of 25-hydroxy-vitamin D3 (calcidiol) level in 1×BB. Loop C (5'-ACTATGGT-3') proved to be the optimal aptamer beacon based on its maximal spread or separation of fluorescence spectra as a function of vitamin D concentration as shown in the upper right quadrant after Loop C was separately synthesized with 5'-TYE 665 dye and 3'-Iowa Black quencher to convert it into a beacon for the titration experiment. The spectra were obtained by serial two-fold dilutions of calcidiol beginning at 100 μg/ml in 1×BB. The candidate VD3 loop beacons were 5' labeled with TYE665 fluorophore and 3' labeled with Iowa Black quencher and HPLC-purified prior to use in assays. Excitation on a spectrofluorometer was at 645 nm with 5 nm slits and a PMT setting of 900 V. Handheld fluorometer values (lower right quadrant) were obtained with an optically modified (red light emitting diode with peak excitation at 650 nm with a 660-720 nm emission filter) Quantifluor®-P device from Promega Corp. and show a lower limit of detection of about 195 ng/ml in 1×BB.
[0067] FIGS. 10A-10C show that the VD3 Loop C beacon appears to react equally well with 1-hydroxy vitamin D2, 1-hydroxy-vitamin D3 and 25-hydroxy-vitamin D3 as assessed by spectrofluorometry in 1×BB (binding buffer). Hence the Loop C beacon is only specific for the vitamin D family and cannot discriminate individual congeners. Cross-reactivity is not problematic since a number of immunoassays for vitamin D detect the total of the major forms of vitamin D2 and D3 together. Hence, the inability of the Loop C VD3 aptamer to discriminate the minor variants of vitamin D is not viewed as a limiting factor. The figures again show serial two-fold dilutions for each of the types of vitamin D beginning at 100 μg/ml in 1×BB with 1-hydroxy-vitamin D2 spectra in the top panel, 1-hydroxy-vitamin D3 spectra in the middle panel, and 25-hydroxy-vitamin D3 spectra in the bottom panel.
[0068] FIG. 11 shows a graphical assessment of the VD3 Loop C beacons by the handheld fluorometer (Quantifluor®-P) in 1×BB across a range of various vitamin D concentrations and for various forms of vitamin D. The handheld reader was modified with a red-emitting (650 nm) LED light source and 660-720 nm emission filter to better work in serum where the red region optics (>600 nm) can avoid much of the blue-green (<600 nm) autofluorescence background of blood, serum or urine. The range shown on the x-axis spans much higher levels than previously shown. Some of the higher levels are not physiological, but might be relevant to detection of vitamin D is some vitamin-rich foods or dairy products. Lines of best fit, whether linear or exponential, are shown for the three different types of vitamin D congeners as well. The highest standard value photodetector setting of 999.0 was used in all cases.
[0069] FIGS. 12A and 12B further assess the specificity or cross-reactivity of the VD3 Loop C aptamer beacon versus a variety of potential analytes or interfering species commonly found in blood and urine by the customized handheld fluorometer (FIG. 12A) and by spectrofluorometry (FIG. 12B) H-DPD; hydroxy-deoxypyridinoline and H-Pyd; hydroxyl-pyridinoline cross linkers from bone.
[0070] FIGS. 13A and 13B show fluorometric spectra from two separate trials of a competitive displacement FRET assay using the VD3 Loop C beacon versus various levels of 25-hydroxy-vitamin D3 (two-fold serial dilutions starting at 100 μg/ml) in 1×BB. In this case, 25-hydroxy-vitamin D3 was labeled with carboxyfluorescein by Fisher esterification (reaction of a hydroxyl group on the vitamin with a carboxyl group on the fluorescein to form a covalent ester bond at acidic pH of approximately 5) followed by binding to appropriately quencher-labeled VD3 aptamer Loop C and competition against levels of calcidiol≦100 micrograms per ml).
[0071] FIG. 14 shows the same samples from FIGS. 13A and 13B assessed for fluorescence peak height by the customized red-emitting handheld fluorometer assessed with the highest standard value photodetector setting of 999.0. The competitive FRET-aptamer assay appears to have a detection limit between 156 to 312 ng/ml in these experiments.
[0072] FIGS. 15A-15D show four examples of aptamers from the SEQ ID NOs 325-526 that bind recombinant human growth hormone (r-hGH; >95% pure research grade) better than the natural form of hGH or somatotropin in ELISA-like assays to help discriminate the artificial form of hGH in potential drug or doping tests for athletes, or to monitor serum levels of exogendus doses of r-hGH administered to children with growth deficits. The ELISA-like assays were conducted with the named aptamers (SEQ ID NOs 336, 338, 343 and 345) instead of antibodies in each case and absorbances were red at 405 nm with an automated microplate reader after a 15 minute development time in the presence of ABTS substrate as is standard in the diagnostics industry.
[0073] FIGS. 16A-16D show four more examples of aptamers (from the SEQ ID NOs 325-526) that bind recombinant human growth hormone ("r-hGH") better than the natural form of hGH or somatotropin to help discriminate the artificial form in potential drug or doping tests for atheletes and growth-challenged children. The ELISA-like assays were conducted with the named aptamers (SEQ ID NOs 348, 377, 379 and 388) instead of antibodies in each case and absorbances were red at 405 nm with an automated microplate reader after a 15 minute development time in the presence of ABTS substrate as is standard in the diagnostics industry.
[0074] FIG. 17A shows an experimental matrix screening scheme for capture aptamer-conjugated magnetic microbeads to be mixed with ruthenium trisbipyridine (Ru(bpy)32+)-labeled reporter aptamers in a sandwich assay scheme to determine which of the 7×7 top hGH and r-hGH aptamer combinations (numbered 1-49 in the scheme table) gave the strongest electrochemiluminescence ("ECL") signal versus 10 pg/ml of r-hGH using an IGEN International Origen® ECL analyzer in phosphate buffered saline containing 0.2 M tripropylamine ("TPA"). ECL was induced by ramping the electrode voltage to 1.25 V.
[0075] FIG. 17B shows the results of this ECL matrix screening process. Mean bar heights of three separate measurements are plotted with standard deviation error bars and the best (most intense ECL) combinations are marked with asterisks.
[0076] FIGS. 18A and 18B give ECL line plots for assay combinations 18 and 40 from the matrix in FIG. 17A as a function of natural hGH concentrations showing sub-picogram and sub-nanogram per ml detection limits and relative linearity over the hGH ranges indicated in 50% human serum (serum diluted 1:1 in 1×BB buffer). The difference in assay regression line slope is due to doubling of the amount of aptamer-coated magnetic beads and reporter aptamer per tube in two different assay trials.
[0077] FIG. 19A shows an experimental matrix screening scheme for capture aptamer-conjugated magnetic microbeads to be mixed with ruthenium trisbipyridine (Ru(bpy)32+)-labeled reporter aptamers in a sandwich assay scheme to determine which of the 4×4 top Brain or B-type Natriuretic Peptide ("BNP") aptamer combinations numbered 1-16 (and selected from the SEQ ID Nos. 527-562) gave the strongest ECL signal versus 100 ng of BNP using an IGEN International Origen® ECL analyzer in phosphate buffered saline containing 0.2 M TPA.
[0078] FIG. 19B shows the results of this ECL matrix screening process. Bar heights represent the means of three separate measurements plotted with standard deviation error bars and the best (most intense ECL) combinations are marked with asterisks.
[0079] FIG. 20A shows the linear ECL response for combination 4 (see FIG. 19A) sandwich assay versus picogram per ml levels of BNP in a 50% human serum and 1×BB diluent environment.
[0080] FIG. 20B shows cross-reactivity of the combination number 4 BNP assay versus other peptide or protein analytes that might be found at the 100 ng/ml level in human blood.
[0081] Although the invention and DNA ligand (aptamer) sequences have been described with reference to specific embodiments, these descriptions are not meant to be construed in a limited sense. Various modifications of the disclosed embodiments, as well as alternative embodiments of the inventions will become apparent to persons skilled in the art upon reference to the description of the invention. It is, therefore, contemplated that the appended claims will cover such modifications that fall within the scope of the invention.
Sequence CWU
1
1
906159DNAArtificial Sequencechemically synthesized 1atacgggagc caacaccact
aacttgttgc tgatcttatc cagagcaggt gtgacggat 59259DNAArtificial
Sequencechemically synthesized 2atccgtcaca cctgctctgg ataagatcag
caacaagtta gtggtgttgg ctcccgtat 59372DNAArtificial
Sequencechemically synthesized 3atacgggagc caacaccacc cgtttttgat
ctaatgagga tacaatattc gtctagagca 60ggtgtgacgg at
72472DNAArtificial Sequencechemically
synthesized 4atccgtcaca cctgctctag acgaatattg tatcctcatt agatcaaaaa
cgggtggtgt 60tggctcccgt at
72572DNAArtificial Sequencechemically synthesized
5atacgggagc caacaccaat cgatggttag actattacac tagatggaat tcatagagca
60ggtgtgacgg at
72672DNAArtificial Sequencechemically synthesized 6atccgtcaca cctgctctat
gaattccatc tagtgtaata gtctaaccat cgattggtgt 60tggctcccgt at
72772DNAArtificial
Sequencechemically synthesized 7atacgggagc caacaccaat ctgccgacta
ggccaagtaa ttatattcag ctggagagca 60ggtgtgacgg at
72872DNAArtificial Sequencechemically
synthesized 8atccgtcaca cctgctctcc agctgaatat aattacttgg cctagtcggc
agattggtgt 60tggctcccgt at
72959DNAArtificial Sequencechemically synthesized
9atacgggagc caacaccaca gctgatattg gatggtccgg cagagcaggt gtgacggat
591059DNAArtificial Sequencechemically synthesized 10atccgtcaca
cctgctctgc cggaccatcc aatatcagct gtggtgttgg ctcccgtat
591172DNAArtificial Sequencechemically synthesized 11atacgggagc
caacaccaca ttacaataga tgtattgaca tatccggaca gtcgagagca 60ggtgtgacgg
at
721272DNAArtificial Sequencechemically synthesized 12atccgtcaca
cctgctctcg actgtccgga tatgtcaata catctattgt aatgtggtgt 60tggctcccgt
at
721372DNAArtificial Sequencechemically synthesized 13atacgggagc
caacaccact cgtgtagtgc tgtctttgtg gaatccttgc atcgagagca 60ggtgtgacgg
at
721472DNAArtificial Sequencechemically synthesized 14atccgtcaca
cctgctctcg atgcaaggat tccacaaaga cagcactaca cgagtggtgt 60tggctcccgt
at
721572DNAArtificial Sequencechemically synthesized 15atacgggagc
caacaccacc acgtgaccca tacgatacaa caaataattg ctcaagagca 60ggtgtgacgg
at
721672DNAArtificial Sequencechemically synthesized 16atccgtcaca
cctgctcttg agcaattatt tgttgtatcg tatgggtcac gtggtggtgt 60tggctcccgt
at
721772DNAArtificial Sequencechemically synthesized 17atacgggagc
caacaccatc catagctcat ctataccctc ttccgagtcc caccagagca 60ggtgtgacgg
at
721872DNAArtificial Sequencechemically synthesized 18atccgtcaca
cctgctctgg tgggactcgg aagagggtat agatgagcta tggatggtgt 60tggctcccgt
at
721972DNAArtificial Sequencechemically synthesized 19atacgggagc
caacaccaga cgcggaacga ctcatcgcaa aatgtcgtga tgcaagagca 60ggtgtgacgg
at
722072DNAArtificial Sequencechemically synthesized 20atccgtcaca
cctgctcttg catcacgaca ttttgcgatg agtcgttccg cgtctggtgt 60tggctcccgt
at
722172DNAArtificial Sequencechemically synthesized 21atacgggagc
caacaccatg gttaggctgc tccatatatt cccgccccgc acgtagagca 60ggtgtgacgg
at
722272DNAArtificial Sequencechemically synthesized 22atccgtcaca
cctgctctac gtgcggggcg ggaatatatg gagcagccta accatggtgt 60tggctcccgt
at
722369DNAArtificial Sequencechemically synthesized 23atacgggagc
caacaccata gtgttgggcc aatacggtaa cgtgtccttg gagagcaggt 60gtgacggat
692469DNAArtificial Sequencechemically synthesized 24atccgtcaca
cctgctctcc aaggacacgt taccgtattg gcccaacact atggtgttgg 60ctcccgtat
692572DNAArtificial Sequencechemically synthesized 25atacgggagc
caacaccatg ctgaactaac agatacaaga tacagatccg agttagagca 60ggtgtgacgg
at
722675DNAArtificial Sequencechemically synthesized 26atccgtcaca
cctgctctaa ctcggatctg tatcttgtat ctgttagttc agcatggtgt 60tggctcccgt
atata
752772DNAArtificial Sequencechemically synthesized 27atacgggagc
caacaccatg tcgctggtca catccctagt taacaaactt ggatagagca 60ggtgtgacgg
at
722872DNAArtificial Sequencechemically synthesized 28atccgtcaca
cctgctctat ccaagtttgt taactaggga tgtgaccagc gacatggtgt 60tggctcccgt
at
722972DNAArtificial Sequencechemically synthesized 29atacgggagc
caacaccatt ggcgttacta gaggattact gaaagccata aactagagca 60ggtgtgacgg
at
723072DNAArtificial Sequencechemically synthesized 30atccgtcaca
cctgctctag tttatggctt tcagtaatcc tctagtaacg ccaatggtgt 60tggctcccgt
at
723172DNAArtificial Sequencechemically synthesized 31atacgggagc
caacaccatc actataattc cttcgaacct tacttgtgtt agctagagca 60ggtgtgacgg
at
723272DNAArtificial Sequencechemically synthesized 32atccgtcaca
cctgctctag ctaacacaag taaggttcga aggaattata gtgatggtgt 60tggctcccgt
at
723372DNAArtificial Sequencechemically synthesized 33atacgggagc
caacaccact atcgactttt acattcgtgt acgattcccg cttcagagca 60ggtgtgacgg
at
723472DNAArtificial Sequencechemically synthesized 34atccgtcaca
cctgctctga agcgggaatc gtacacgaat gtaaaagtcg atagtggtgt 60tggctcccgt
at
723572DNAArtificial Sequencechemically synthesized 35atacgggagc
caacaccatc ccctcgggcg agctatacta aaccagatcc taagagagca 60ggtgtgacgg
at
723672DNAArtificial Sequencechemically synthesized 36atccgtcaca
cctgctctct taggatctgg tttagtatag ctcgcccgag gggatggtgt 60tggctcccgt
at
723772DNAArtificial Sequencechemically synthesized 37atacgggagc
caacaccaag ctacacaacg aggcgtataa tcagcatgtt ggatagagca 60ggtgtgacgg
at
723872DNAArtificial Sequencechemically synthesized 38atccgtcaca
cctgctctat ccaacatgct gattatacgc ctcgttgtgt agcttggtgt 60tggctcccgt
at
723972DNAArtificial Sequencechemically synthesized 39atacgggagc
caacaccagg aaactactaa acgaacatga gctgtatcaa aaacagagca 60ggtgtgacgg
at
724072DNAArtificial Sequencechemically synthesized 40atccgtcaca
cctgctctgt ttttgataca gctcatgttc gtttagtagt ttcctggtgt 60tggctcccgt
at
724172DNAArtificial Sequencechemically synthesized 41atacgggagc
caacaccatc cagagtaaac gataaggggg aatctcaatg aaccagagca 60ggtgtgacgg
at
724272DNAArtificial Sequencechemically synthesized 42atccgtcaca
cctgctctgg ttcattgaga ttccccctta tcgtttactc tggatggtgt 60tggctcccgt
at
724372DNAArtificial Sequencechemically synthesized 43atacgggagc
caacaccaat actctcaaaa cctaacaaga caacccgagg gcccagagca 60ggtgtgacgg
at
724472DNAArtificial Sequencechemically synthesized 44atccgtcaca
cctgctctgg gccctcgggt tgtcttgtta ggttttgaga gtattggtgt 60tggctcccgt
at
724572DNAArtificial Sequencechemically synthesized 45atacgggagc
caacaccatg agactgggtc gatccaggac atacatttgt actaagagca 60ggtgtgacgg
at
724672DNAArtificial Sequencechemically synthesized 46atccgtcaca
cctgctctta gtacaaatgt atgtcctgga tcgacccagt ctcatggtgt 60tggctcccgt
at
724772DNAArtificial Sequencechemically synthesized 47atacgggagc
caacaccaga gccagaataa tggccccctt tacgagacga ctgtagagca 60ggtgtgacgg
at
724872DNAArtificial Sequencechemically synthesized 48atccgtcaca
cctgctctac agtcgtctcg taaagggggc cattattctg gctctggtgt 60tggctcccgt
at
724972DNAArtificial Sequencechemically synthesized 49atacgggagc
caacaccatc aggccgaaat gtatacgaag gtaagcgata aagtagagca 60ggtgtgacgg
at
725072DNAArtificial Sequencechemically synthesized 50atccgtcaca
cctgctctac tttatcgctt accttcgtat acatttcggc ctgatggtgt 60tggctcccgt
at
725172DNAArtificial Sequencechemically synthesized 51atacgggagc
caacaccagt cgtacgacac ccatccaaga ctttattccg cctaagagca 60ggtgtgacgg
at
725272DNAArtificial Sequencechemically synthesized 52atccgtcaca
cctgctctta ggcggaataa agtcttggat gggtgtcgta cgactggtgt 60tggctcccgt
at
725372DNAArtificial Sequencechemically synthesized 53atacgggagc
caacaccacg gtacaaactc tcaacaagct accactttag tccaagagca 60ggtgtgacgg
at
725472DNAArtificial Sequencechemically synthesized 54atccgtcaca
cctgctcttg gactaaagtg gtagcttgtt gagagtttgt accgtggtgt 60tggctcccgt
at
725572DNAArtificial Sequencechemically synthesized 55atacgggagc
caacaccact cgtttggccc acgagtcaat catatatcag ctaaagagca 60ggtgtgacgg
at
725672DNAArtificial Sequencechemically synthesized 56atccgtcaca
cctgctcttt agctgatata tgattgactc gtgggccaaa cgagtggtgt 60tggctcccgt
at
725772DNAArtificial Sequencechemically synthesized 57atacgggagc
caacaccatt attatcaaga cgctatctac agagattcac ggacagagca 60ggtgtgacgg
at
725872DNAArtificial Sequencechemically synthesized 58atccgtcaca
cctgctctgt ccgtgaatct ctgtagatag cgtcttgata ataatggtgt 60tggctcccgt
at
725972DNAArtificial Sequencechemically synthesized 59atacgggagc
caacaccagt gcgtgcgtgg cgacggtact actgactatg tcgtagagca 60ggtgtgacgg
at
726072DNAArtificial Sequencechemically synthesized 60atccgtcaca
cctgctctac gacatagtca gtagtaccgt cgccacgcac gcactggtgt 60tggctcccgt
at
726172DNAArtificial Sequencechemically synthesized 61atacgggagc
caacaccatg ccattcctgt gttagtctgt ccgttcgaca aggcagagca 60ggtgtgacgg
at
726272DNAArtificial Sequencechemically synthesized 62atccgtcaca
cctgctctgc cttgtcgaac ggacagacta acacaggaat ggcatggtgt 60tggctcccgt
at
726372DNAArtificial Sequencechemically synthesized 63atacgggagc
caacaccaga gtgttggcga taaacacaat gacccacagc cggaagagca 60ggtgtgacgg
at
726472DNAArtificial Sequencechemically synthesized 64atccgtcaca
cctgctcttc cggctgtggg tcattgtgtt tatcgccaac actctggtgt 60tggctcccgt
at
726572DNAArtificial Sequencechemically synthesized 65atacgggagc
caacaccatt ctggtgcgcg tgcaatgaga gaaatggctc gtacagagca 60ggtgtgacgg
at
726672DNAArtificial Sequencechemically synthesized 66atccgtcaca
cctgctctgt acgagccatt tctctcattg cacgcgcacc agaatggtgt 60tggctcccgt
at
726772DNAArtificial Sequencechemically synthesized 67atacgggagc
caacaccacc gcgttaaaga ggcgtacgat gtctcagtct cgccagagca 60ggtgtgacgg
at
726872DNAArtificial Sequencechemically synthesized 68atccgtcaca
cctgctctgg cgagactgag acatcgtacg cctctttaac gcggtggtgt 60tggctcccgt
at
726972DNAArtificial Sequencechemically synthesized 69atacgggagc
caacaccatt ctggtgcgcg tgcaatgaga gaaatggctc gtacagagca 60ggtgtgacgg
at
727072DNAArtificial Sequencechemically synthesized 70atccgtcaca
cctgctctgt acgagccatt tctctcattg cacgcgcacc agaatggtgt 60tggctcccgt
at
727172DNAArtificial Sequencechemically synthesized 71atacgggagc
caacaccacc gcgttaaaga ggcgtacgat gtctcagtct cgccagagca 60ggtgtgacgg
at
727272DNAArtificial Sequencechemically synthesized 72atccgtcaca
cctgctctgg cgagactgag acatcgtacg cctctttaac gcggtggtgt 60tggctcccgt
at
727372DNAArtificial Sequencechemically synthesized 73atacgggagc
caacaccatc taactgatgt gttgcgaagg gtctgctgtg tgatagagca 60ggtgtgacgg
at
727472DNAArtificial Sequencechemically synthesized 74atccgtcaca
cctgctctat cacacagcag acccttcgca acacatcagt tagatggtgt 60tggctcccgt
at
727572DNAArtificial Sequencechemically synthesized 75atacgggagc
caacaccagg cgggagaata gatagagtta gtcgtagtta gagcagagca 60ggtgtgacgg
at
727672DNAArtificial Sequencechemically synthesized 76atccgtcaca
cctgctctgc tctaactacg actaactcta tctattctcc cgcctggtgt 60tggctcccgt
at
727772DNAArtificial Sequencechemically synthesized 77atacgggagc
caacaccaga agaaccgcta aagcctatga ggaaaggatc atgcagagca 60ggtgtgacgg
at
727872DNAArtificial Sequencechemically synthesized 78atccgtcaca
cctgctctgc atgatccttt cctcataggc tttagcggtt cttctggtgt 60tggctcccgt
at
727972DNAArtificial Sequencechemically synthesized 79atacgggagc
caacaccagc cacgtcgaac cgggtgtcca tatacgtaat atgcagagca 60ggtgtgacgg
at
728072DNAArtificial Sequencechemically synthesized 80atccgtcaca
cctgctctgc atattacgta tatggacacc cggttcgacg tggctggtgt 60tggctcccgt
at
728172DNAArtificial Sequencechemically synthesized 81atacgggagc
caacaccatg ccatgtaccg gtacctactc tatcaggata taggagagca 60ggtgtgacgg
at
728272DNAArtificial Sequencechemically synthesized 82atccgtcaca
cctgctctcc tatatcctga tagagtaggt accggtacat ggcatggtgt 60tggctcccgt
at
728372DNAArtificial Sequencechemically synthesized 83atacgggagc
caacaccaga gtattccgct cacctatcgc caccatgtac accaagagca 60ggtgtgacgg
at
728472DNAArtificial Sequencechemically synthesized 84atccgtcaca
cctgctcttg gtgtacatgg tggcgatagg tgagcggaat actctggtgt 60tggctcccgt
at
728572DNAArtificial Sequencechemically synthesized 85atacgggagc
caacaccaac attgagagtg tggacatagt ggaacgcgaa cctgagagca 60ggtgtgacgg
at
728672DNAArtificial Sequencechemically synthesized 86atccgtcaca
cctgctctca ggttcgcgtt ccactatgtc cacactctca atgttggtgt 60tggctcccgt
at
728772DNAArtificial Sequencechemically synthesized 87atacgggagc
caacaccaag cgacaaggaa gaattatcaa ggtacgaacc ggatagagca 60ggtgtgacgg
at
728872DNAArtificial Sequencechemically synthesized 88atccgtcaca
cctgctctat ccggttcgta ccttgataat tcttccttgt cgcttggtgt 60tggctcccgt
at
728972DNAArtificial Sequencechemically synthesized 89atacgggagc
caacaccatg tcatcatata ccaacactgg cgcggatagt ccagagagca 60ggtgtgacgg
at
729072DNAArtificial Sequencechemically synthesized 90atccgtcaca
cctgctctct ggactatccg cgccagtgtt ggtatatgat gacatggtgt 60tggctcccgt
at
729172DNAArtificial Sequencechemically synthesized 91atacgggagc
caacaccagc ctcgtgattt gatgaagttt cttgatctgc acgtagagca 60ggtgtgacgg
at
729272DNAArtificial Sequencechemically synthesized 92atccgtcaca
cctgctctac gtgcagatca agaaacttca tcaaatcacg aggctggtgt 60tggctcccgt
at
729372DNAArtificial Sequencechemically synthesized 93atacgggagc
caacaccatc gatgaacggc tcaccctcgt ccctatataa cactagagca 60ggtgtgacgg
at
729472DNAArtificial Sequencechemically synthesized 94atccgtcaca
cctgctctag tgttatatag ggacgagggt gagccgttca tcgatggtgt 60tggctcccgt
at
729572DNAArtificial Sequencechemically synthesized 95atacgggagc
caacaccaaa ggacacaaga agcttgaatc tccagttagt ggggagagca 60ggtgtgacgg
at
729672DNAArtificial Sequencechemically synthesized 96atccgtcaca
cctgctctcc ccactaactg gagattcaag cttcttgtgt cctttggtgt 60tggctcccgt
at
729772DNAArtificial Sequencechemically synthesized 97atacgggagc
caacaccacg ctccccgaga tcacgtacgc tcgccccaga ctggagagca 60ggtgtgacgg
at
729872DNAArtificial Sequencechemically synthesized 98atccgtcaca
cctgctctcc agtctggggc gagcgtacgt gatctcgggg agcgtggtgt 60tggctcccgt
at
729972DNAArtificial Sequencechemically synthesized 99atacgggagc
caacaccatg gctcaccagg accattattt tcagctcccc cgccagagca 60ggtgtgacgg
at
7210072DNAArtificial Sequencechemically synthesized 100atccgtcaca
cctgctctgg cgggggagct gaaaataatg gtcctggtga gccatggtgt 60tggctcccgt
at
7210172DNAArtificial Sequencechemically synthesized 101atacgggagc
caacaccagt gcaccaaata aaaagctgtc ccagactcga gcgaagagca 60ggtgtgacgg
at
7210272DNAArtificial Sequencechemically synthesized 102atccgtcaca
cctgctcttc gctcgagtct gggacagctt tttatttggt gcactggtgt 60tggctcccgt
at
7210372DNAArtificial Sequencechemically synthesized 103atacgggagc
caacaccaat cgtgtcacaa tggcatatcc tacgtcataa gccaagagca 60ggtgtgacgg
at
7210472DNAArtificial Sequencechemically synthesized 104atccgtcaca
cctgctcttg gcttatgacg taggatatgc cattgtgaca cgattggtgt 60tggctcccgt
at
7210572DNAArtificial Sequencechemically synthesized 105atacgggagc
caacaccata ataccagccg acagccttgg tgctatgatt tgacagagca 60ggtgtgacgg
at
7210672DNAArtificial Sequencechemically synthesized 106atccgtcaca
cctgctctgt caaatcatag caccaaggct gtcggctggt attatggtgt 60tggctcccgt
at
7210772DNAArtificial Sequencechemically synthesized 107atacgggagc
caacaccatc gagtgcgtgg aactcaaggg cacaatagct acacagagca 60ggtgtgacgg
at
7210872DNAArtificial Sequencechemically synthesized 108atccgtcaca
cctgctctgt gtagctattg tgcccttgag ttccacgcac tcgatggtgt 60tggctcccgt
at
7210972DNAArtificial Sequencechemically synthesized 109atacgggagc
caacaccaca tagtccctgt cgcacccctc ccgtgaccgt tatcagagca 60ggtgtgacgg
at
7211072DNAArtificial Sequencechemically synthesized 110atccgtcaca
cctgctctga taacggtcac gggaggggtg cgacagggac tatgtggtgt 60tggctcccgt
at
7211172DNAArtificial Sequencechemically synthesized 111atacgggagc
caacaccagg cgttctagag accactctta ataaccttga tgtgagagca 60ggtgtgacgg
at
7211272DNAArtificial Sequencechemically synthesized 112atccgtcaca
cctgctctca catcaaggtt attaagagtg gtctctagaa cgcctggtgt 60tggctcccgt
at
7211372DNAArtificial Sequencechemically synthesized 113atacgggagc
caacaccagt ttctcagtat ataagatatt tcatcgcagg tagaagagca 60ggtgtgacgg
at
7211472DNAArtificial Sequencechemically synthesized 114atccgtcaca
cctgctcttc tacctgcgat gaaatatctt atatactgag aaactggtgt 60tggctcccgt
at
7211572DNAArtificial Sequencechemically synthesized 115atacgggagc
caacaccaat gtgacagtcg cgactcctgt cgtttgcata gggtagagca 60ggtgtgacgg
at
7211672DNAArtificial Sequencechemically synthesized 116atccgtcaca
cctgctctac cctatgcaaa cgacaggagt cgcgactgtc acattggtgt 60tggctcccgt
at
7211772DNAArtificial Sequencechemically synthesized 117atacgggagc
caacaccaaa aaatcaacat ggtgaaacat tcgaatgcag ttgaagagca 60ggtgtgacgg
at
7211872DNAArtificial Sequencechemically synthesized 118atccgtcaca
cctgctcttc aactgcattc gaatgtttca ccatgttgat tttttggtgt 60tggctcccgt
at
7211972DNAArtificial Sequencechemically synthesized 119atacgggagc
caacaccatg cgcggctcat agactgtaac ccggatggga acctagagca 60ggtgtgacgg
at
7212072DNAArtificial Sequencechemically synthesized 120atccgtcaca
cctgctctag gttcccatcc gggttacagt ctatgagccg cgcatggtgt 60tggctcccgt
at
7212172DNAArtificial Sequencechemically synthesized 121atacgggagc
caacaccaag tcccgttacc aaacagaacc atggagagcg atggagagca 60ggtgtgacgg
at
7212272DNAArtificial Sequencechemically synthesized 122atccgtcaca
cctgctctcc atcgctctcc atggttctgt ttggtaacgg gacttggtgt 60tggctcccgt
at
7212372DNAArtificial Sequencechemically synthesized 123atacgggagc
caacaccatt gacccgagac tccagctgag tgacacctga tgtgagagca 60ggtgtgacgg
at
7212472DNAArtificial Sequencechemically synthesized 124atccgtcaca
cctgctctca catcaggtgt cactcagctg gagtctcggg tcaatggtgt 60tggctcccgt
at
7212572DNAArtificial Sequencechemically synthesized 125atacgggagc
caacaccagt gacaccaaac catgctcagg ccattcggtt atatagagca 60ggtgtgacgg
at
7212672DNAArtificial Sequencechemically synthesized 126atccgtcaca
cctgctctat ataaccgaat ggcctgagca tggtttggtg tcactggtgt 60tggctcccgt
at
7212772DNAArtificial Sequencechemically synthesized 127atacgggagc
caacaccagc caacggacac caggttgatc aatccccagt cctgagagca 60ggtgtgacgg
at
7212872DNAArtificial Sequencechemically synthesized 128atccgtcaca
cctgctctca ggactgggga ttgatcaacc tggtgtccgt tggctggtgt 60tggctcccgt
at
7212972DNAArtificial Sequencechemically synthesized 129atacgggagc
caacaccagc cctaccgtac cccctcaaga cagaacctgc atcaagagca 60ggtgtgacgg
at
7213072DNAArtificial Sequencechemically synthesized 130atccgtcaca
cctgctcttg atgcaggttc tgtcttgagg gggtacggta gggctggtgt 60tggctcccgt
at
7213172DNAArtificial Sequencechemically synthesized 131atacgggagc
caacaccact agacaaggtc aaaccgcata tggacccgtg ctatagagca 60ggtgtgacgg
at
7213272DNAArtificial Sequencechemically synthesized 132atccgtcaca
cctgctctat agcacgggtc catatgcggt ttgaccttgt ctagtggtgt 60tggctcccgt
at
7213372DNAArtificial Sequencechemically synthesized 133atacgggagc
caacaccaca acatgagctg aatctgtccc taatgcgtgt gcatagagca 60ggtgtgacgg
at
7213472DNAArtificial Sequencechemically synthesized 134atccgtcaca
cctgctctat gcacacgcat tagggacaga ttcagctcat gttgtggtgt 60tggctcccgt
at
7213572DNAArtificial Sequencechemically synthesized 135atacgggagc
caacaccacg gtgaagcatg gcaatattaa ccatgatcac cttcagagca 60ggtgtgacgg
at
7213672DNAArtificial Sequencechemically synthesized 136atccgtcaca
cctgctctga aggtgatcat ggttaatatt gccatgcttc accgtggtgt 60tggctcccgt
at
7213772DNAArtificial Sequencechemically synthesized 137atacgggagc
caacaccacc acttgtcacg accgtgtctc tgtggcgctt ggggagagca 60ggtgtgacgg
at
7213872DNAArtificial Sequencechemically synthesized 138atccgtcaca
cctgctctcc ccaagcgcca cagagacacg gtcgtgacaa gtggtggtgt 60tggctcccgt
at
7213972DNAArtificial Sequencechemically synthesized 139atacgggagc
caacaccata tagccgcgcc tgtgagtttt gtgggagcaa gagtagagca 60ggtgtgacgg
at
7214072DNAArtificial Sequencechemically synthesized 140atccgtcaca
cctgctctac tcttgctccc acaaaactca caggcgcggc tatatggtgt 60tggctcccgt
at
7214172DNAArtificial Sequencechemically synthesized 141atacgggagc
caacaccagc tacagtgtca gacggttcca ccttaacctc gtcaagagca 60ggtgtgacgg
at
7214272DNAArtificial Sequencechemically synthesized 142atccgtcaca
cctgctcttg acgaggttaa ggtggaaccg tctgacactg tagctggtgt 60tggctcccgt
at
7214372DNAArtificial Sequencechemically synthesized 143atacgggagc
caacaccatt gactaagcga ttagtcccac aggtgaccgg ggagagagca 60ggtgtgacgg
at
7214472DNAArtificial Sequencechemically synthesized 144atccgtcaca
cctgctctct tcccggnctc ctgtgtgatt aatctgttat tctatggtgt 60tggctcccgt
at
7214572DNAArtificial Sequencechemically synthesized 145atacgggagc
caacaccaat tctaacacag gtttctccgt ttcgttagct gctaagagca 60ggtgtgacgg
at
7214672DNAArtificial Sequencechemically synthesized 146atccgtcaca
cctgctctta gcagctaacg aaacggagaa acctgtgtta gaattggtgt 60tggctcccgt
at
7214772DNAArtificial Sequencechemically synthesized 147atacgggagc
caacaccact catcccgttg gaacacttta atatggccca ctctagagca 60ggtgtgacgg
at
7214872DNAArtificial Sequencechemically synthesized 148atccgtcaca
cctgctctag agtgggccat attaaagtgt tccaacggga tgagtggtgt 60tggctcccgt
at
7214972DNAArtificial Sequencechemically synthesized 149atacgggagc
caacaccact actgtgcaga tccagcatct acggtgaaat ttgcagagca 60ggtgtgacgg
at
7215072DNAArtificial Sequencechemically synthesized 150atccgtcaca
cctgctctgc aaatttcacc gtagatgctg gatctgcaca gtagtggtgt 60tggctcccgt
at
7215172DNAArtificial Sequencechemically synthesized 151atacgggagc
caacaccatg cggaaagccc ataccaaccc agaagccagc gctaagagca 60ggtgtgacgg
at
7215272DNAArtificial Sequencechemically synthesized 152atccgtcaca
cctgctctta gcgctggctt ctgggttggt atgggctttc cgcatggtgt 60tggctcccgt
at
7215372DNAArtificial Sequencechemically synthesized 153atacgggagc
caacaccagc caactagcct gaaacccata attatacagc ttagagagca 60ggtgtgacgg
at
7215472DNAArtificial Sequencechemically synthesized 154atccgtcaca
cctgctctct aagctgtata attatgggtt tcaggctagt tggctggtgt 60tggctcccgt
at
7215572DNAArtificial Sequencechemically synthesized 155atacgggagc
caacaccact gtatccgttg atgacaaaca gtcgcagccc acttagagca 60ggtgtgacgg
at
7215672DNAArtificial Sequencechemically synthesized 156atccgtcaca
cctgctctcc tcgactcgat ttcacttagt ctaacactaa ttggtggtgt 60tggctcccgt
at
7215772DNAArtificial Sequencechemically synthesized 157atacgggagc
caacaccaag gtaatgaagg gggaaaatgg agttttgccc ttaaagagca 60ggtgtgacgg
at
7215872DNAArtificial Sequencechemically synthesized 158atccgtcaca
cctgctcttt aagggcaaaa ctccattttc ccccttcatt accttggtgt 60tggctcccgt
at
7215972DNAArtificial Sequencechemically synthesized 159atacgggagc
caacaccaag ctcgtagaag catcttacgc ggcgcccttg caccagagca 60ggtgtgacgg
at
7216072DNAArtificial Sequencechemically synthesized 160atccgtcaca
cctgctctgg tgcaagggcg ccgcgtaaga tgcttctacg agcttggtgt 60tggctcccgt
at
7216172DNAArtificial Sequencechemically synthesized 161atacgggagc
caacaccacg atcattgaca tacttctcta tcccgtctgt ctgtagagca 60ggtgtgacgg
at
7216272DNAArtificial Sequencechemically synthesized 162atccgtcaca
cctgctctac agacagacgg gatagagaag tatgtcaatg atcgtggtgt 60tggctcccgt
at
7216372DNAArtificial Sequencechemically synthesized 163atacgggagc
caacaccacc acagagtcta cccaaggcag gctgtcaatc aacaagagca 60ggtgtgacgg
at
7216472DNAArtificial Sequencechemically synthesized 164atccgtcaca
cctgctcttg ttgattgaca gcctgccttg ggtagactct gtggtggtgt 60tggctcccgt
at
7216572DNAArtificial Sequencechemically synthesized 165atacgggagc
caacaccagg ctacgacaaa ggctatgaca tgaatgggtc aactagagca 60ggtgtgacgg
at
7216672DNAArtificial Sequencechemically synthesized 166atccgtcaca
cctgctctag ttgacccatt catgtcatag cctttgtcgt agcctggtgt 60tggctcccgt
at
7216772DNAArtificial Sequencechemically synthesized 167atacgggagc
caacaccagg gccggagggt tcttcttgag ccttttcgcc aggcagagca 60ggtgtgacgg
at
7216872DNAArtificial Sequencechemically synthesized 168atccgtcaca
cctgctctgc ctggcgaaaa ggctcaagaa gaaccctccg gccctggtgt 60tggctcccgt
at
7216972DNAArtificial Sequencechemically synthesized 169atacgggagc
caacaccaaa tgtcttggaa ggcagatatc ggaacgagtg gaccagagca 60ggtgtgacgg
at
7217072DNAArtificial Sequencechemically synthesized 170atccgtcaca
cctgctctgg tccactcgtt ccgatatctg ccttccaaga catttggtgt 60tggctcccgt
at
7217172DNAArtificial Sequencechemically synthesized 171atacgggagc
caacaccaga cactcaggta tggagcactc gatccacgcg tgtgagagca 60ggtgtgacgg
at
7217272DNAArtificial Sequencechemically synthesized 172atccgtcaca
cctgctctca cacgcgtgga tcgagtgctc catacctgag tgtctggtgt 60tggctcccgt
at
7217372DNAArtificial Sequencechemically synthesized 173atacgggagc
caacaccatc cgcgaccaga atctgtaaag gcgttagata tgccagagca 60ggtgtgacgg
at
7217472DNAArtificial Sequencechemically synthesized 174atccgtcaca
cctgctctgg catatctaac gcctttacag attctggtcg cggatggtgt 60tggctcccgt
at
7217572DNAArtificial Sequencechemically synthesized 175atacgggagc
caacaccact caacgtaacg ggtggagacc ctcacatcga tatgagagca 60ggtgtgacgg
at
7217672DNAArtificial Sequencechemically synthesized 176atccgtcaca
cctgctctca tatcgatgtg agggtctcca cccgttacgt tgagtggtgt 60tggctcccgt
at
7217772DNAArtificial Sequencechemically synthesized 177atacgggagc
caacaccaga tggtgaccgg attacgagaa ccagagctga tagaagagca 60ggtgtgacgg
at
7217872DNAArtificial Sequencechemically synthesized 178atccgtcaca
cctgctcttc tatcagctct ggttctcgta atccggtcac catctggtgt 60tggctcccgt
at
7217972DNAArtificial Sequencechemically synthesized 179atacgggagc
caacaccaaa ccagggaata cacagcaaaa ctacggtgtc ctgtagagca 60ggtgtgacgg
at
7218072DNAArtificial Sequencechemically synthesized 180atccgtcaca
cctgctctac aggacaccgt agttttgctg tgtattccct ggtttggtgt 60tggctcccgt
at
7218172DNAArtificial Sequencechemically synthesized 181atacgggagc
caacaccagc cgataaagaa cgcagctaat ttgctctccc gagcagagca 60ggtgtgacgg
at
7218272DNAArtificial Sequencechemically synthesized 182atccgtcaca
cctgctctgc tcgggagagc aaattagctg cgttctttat cggctggtgt 60tggctcccgt
at
7218372DNAArtificial Sequencechemically synthesized 183atacgggagc
caacaccagc atatgtagtg aagcgacata caccggggca acgtagagca 60ggtgtgacgg
at
7218472DNAArtificial Sequencechemically synthesized 184atccgtcaca
cctgctctac gttgccccgg tgtatgtcgc ttcactacat atgctggtgt 60tggctcccgt
at
7218572DNAArtificial Sequencechemically synthesized 185atacgggagc
caacaccacc gaactaagag gaaagtctcg gagtgcggct agttagagca 60ggtgtgacgg
at
7218672DNAArtificial Sequencechemically synthesized 186atccgtcaca
cctgctctaa ctagccgcac tccgagactt tcctcttagt tcggtggtgt 60tggctcccgt
at
7218772DNAArtificial Sequencechemically synthesized 187atacgggagc
caacaccaag gccacccagt taatgcagtc tcgttatgtt ctgtagagca 60ggtgtgacgg
at
7218872DNAArtificial Sequencechemically synthesized 188atccgtcaca
cctgctctac agaacataac gagactgcat taactgggtg gccttggtgt 60tggctcccgt
at
7218972DNAArtificial Sequencechemically synthesized 189atacgggagc
caacaccaat accatacctg acttatgacc tgatcatacc agtgagagca 60ggtgtgacgg
at
7219072DNAArtificial Sequencechemically synthesized 190atccgtcaca
cctgctctca ctggtatgat caggtcataa gtcaggtatg gtattggtgt 60tggctcccgt
at
7219171DNAArtificial Sequencechemically synthesized 191atacgggagc
cagcaccaca gattcttcaa ctttgctgcc atattccaac aacagagcag 60gcgtgacgga t
7119271DNAArtificial Sequencechemically synthesized 192atccgtcacg
cctgctctgt tgttggaata tggcagcaaa gttgaagaat ctgtggtgct 60ggctcccgta t
7119372DNAArtificial Sequencechemically synthesized 193atacgggagc
caacaccaca tgccataatt ggaccctcat cgccccgtcg tcccagagca 60ggtgtgacgg
at
7219472DNAArtificial Sequencechemically synthesized 194atccgtcaca
cctgctctgg gacgacgggg cgatgagggt ccaattatgg catgtggtgt 60tggctcccgt
at
7219572DNAArtificial Sequencechemically synthesized 195atacgggagc
caacaccaaa ccgcaccagt cacacaccag ttcgcctcca tgttagagca 60ggtgtgacgg
at
7219672DNAArtificial Sequencechemically synthesized 196atccgtcaca
cctgctctaa catggaggcg aactggtgtg tgactggtgc ggtttggtgt 60tggctcccgt
at
7219772DNAArtificial Sequencechemically synthesized 197atacgggagc
caacaccatt ttacagtact aaagtatgtg atatgccgcg ccggagagca 60ggtgtgacgg
at
7219872DNAArtificial Sequencechemically synthesized 198atccgtcaca
cctgctctcc ggcgcggcat atcacatact ttagtactgt aaaatggtgt 60tggctcccgt
at
7219972DNAArtificial Sequencechemically synthesized 199atacgggagc
caacaccaaa agaacgcact actttcggaa tcgaatctat ccccagagca 60ggtgtgacgg
at
7220072DNAArtificial Sequencechemically synthesized 200atccgtcaca
cctgctctgg ggatagattc gattccgaaa gtagtgcgtt cttttggtgt 60tggctcccgt
at
7220172DNAArtificial Sequencechemically synthesized 201atccgtcaca
cctgctctag acgaatattg tatcctcatt agatcaaaaa cgggtggtgt 60tggctcccgt
at
7220272DNAArtificial Sequencechemically synthesized 202atacgggagc
caacaccagc ttttcctaga atgattttct ttagctacct gagaagagca 60ggtgtgacgg
at
7220372DNAArtificial Sequencechemically synthesized 203atccgtcaca
cctgctcttc tcaggtagct aaagaaaatc attctaggaa aagctggtgt 60tggctcccgt
at
7220458DNAArtificial Sequencechemically synthesized 204atacgggagc
caacaccacg cttagatatt atccttgtcc agagcaggtg tgacggat
5820558DNAArtificial Sequencechemically synthesized 205atccgtcaca
cctgctctgg acaaggataa tatctaagcg tggtgttggc tcccgtat
5820672DNAArtificial Sequencechemically synthesized 206acacgggagc
caacaccatc catagctcat ctataccctc ttccgagtcc caccagagca 60ggtgtgacgg
at
7220772DNAArtificial Sequencechemically synthesized 207atccgtcaca
cctgctctgg tgggactcgg aagagggtat agatgagcta tggatggtgt 60tggctcccgt
gt
7220872DNAArtificial Sequencechemically synthesized 208atacgggagc
caacaccacc ctacaccagc gccctacact tttgtagcac ttcgagagca 60ggtgtgacgg
at
7220972DNAArtificial Sequencechemically synthesized 209atccgtcaca
cctgctctcg aagtgctaca aaagtgtagg gcgctggtgt agggtggtgt 60tggctcccgt
at
7221069DNAArtificial Sequencechemically synthesized 210atacgggagc
caacaccata gtgttgggcc aatacggtaa cgtgtccttg gagagcaggt 60gtgacggat
6921169DNAArtificial Sequencechemically synthesized 211atccgtcaca
cctgctctcc aaggacacgt taccgtattg gcccaacact atggtgttgg 60ctcccgtat
6921272DNAArtificial Sequencechemically synthesized 212atacgggagc
caacaccacc cgtttttgat ctaatgagga tacaatattc gtctagagca 60ggtgtgacgg
at
7221372DNAArtificial Sequencechemically synthesized 213atacgggagc
caacaccact atcaatttgt tggcgacact tcaacccaca cgttagagca 60ggtgtgacgg
at
7221472DNAArtificial Sequencechemically synthesized 214atccgtcaca
cctgctctaa cgtgtgggtt gaagtgtcgc caacaaattg atagtggtgt 60tggctcccgt
at
7221572DNAArtificial Sequencechemically synthesized 215atacgggagc
caacaccatc agcatgggtt agagctgggg gaaatatttg gtcgagagca 60ggtgtgacgg
at
7221672DNAArtificial Sequencechemically synthesized 216atccgtcaca
cctgctctcg accaaatatt tcccccagct ctaacccatg ctgatggtgt 60tggctcccgt
at
7221769DNAArtificial Sequencechemically synthesized 217atacgggagc
caacaccata gtgttgggcc aatacggtaa cgtgtccttg gagagcaggt 60gtgacggat
6921869DNAArtificial Sequencechemically synthesized 218atccgtcaca
cctgctctcc aaggacacgt taccgtattg gcccaacact atggtgttgg 60ctcccgtat
6921972DNAArtificial Sequencechemically synthesized 219atacgggagc
caacaccaac aaaacaacag gaatatcgtt cccaagcgga ccccagagca 60ggtgtgacgg
at
7222072DNAArtificial Sequencechemically synthesized 220atccgtcaca
cctgctctgg ggtccgcttg ggaacgatat tcctgttgtt ttgttggtgt 60tggctcccgt
at
7222172DNAArtificial Sequencechemically synthesized 221atacgggagc
caacaccata caaagtgttg ttagatttaa cccatgttgc catcagagca 60ggtgtgacgg
at
7222272DNAArtificial Sequencechemically synthesized 222atccgtcaca
cctgctctga tggcaacatg ggttaaatct aacaacactt tgtatggtgt 60tggctcccgt
at
7222372DNAArtificial Sequencechemically synthesized 223atacgggagc
caacaccaag ggtgttcaca ctggcaggcg acgccctcgt gttgagagca 60ggtgtgacgg
at
7222473DNAArtificial Sequencechemically synthesized 224atccgtcaca
cctgctctca acacgagggc gtcgcctgcc agtgtgaaca cccttggtgt 60tggctcccgt
atn
7322572DNAArtificial Sequencechemically synthesized 225atacgggagc
caacaccaca atagtcgatt agtaatgatc cacacattgg tcggagagca 60ggtgtgacgg
at
7222672DNAArtificial Sequencechemically synthesized 226atccgtcaca
cctgctctcc gaccaatgtg tggatcatta ctaatcgact attgtggtgt 60tggctcccgt
at
7222769DNAArtificial Sequencechemically synthesized 227atacgggagc
caacaccata gttttgggcc aatacggtaa cgtgtccttg gagagcaggt 60gtgacggat
6922869DNAArtificial Sequencechemically synthesized 228atccgtcaca
cctgctctcc aaggacacgt taccgtattg gcccaaaact atggtgttgg 60ctcccgtat
6922969DNAArtificial Sequencechemically synthesized 229atacgggagc
caacaccata gtgctggacc aatacggtaa cgtgtccttg gagagcaggt 60gtgacggat
6923069DNAArtificial Sequencechemically synthesized 230atccgtcaca
cctgctctcc aaggacacgt taccgtattg gtccagcact atggtgttgg 60ctcccgtat
6923158DNAArtificial Sequencechemically synthesized 231atacgggagc
caacaccacg attagcaatg aattatctac agagcaggtg tgacggat
5823258DNAArtificial Sequencechemically synthesized 232atccgtcaca
cctgctctgt agataattca ttgctaatcg tggtgttggc tcccgtat
5823372DNAArtificial Sequencechemically synthesized 233atacgggagc
caacaccaat tctaacacag gtttctccgt ttcgttagct gctaagagca 60ggtgtgacgg
at
7223472DNAArtificial Sequencechemically synthesized 234atccgtcaca
cctgctctta gcagctaacg aaacggagaa acctgtgtta gaattggtgt 60tggctcccgt
at
7223572DNAArtificial Sequencechemically synthesized 235atacgggagc
caacaccatt ctatcgttcc ggacgcttat gccttgccat ctacagagca 60ggtgtgacgg
at
7223672DNAArtificial Sequencechemically synthesized 236atccgtcaca
cctgctctgt agatggcaag gcataagcgt ccggaacgat agaatggtgt 60tggctcccgt
at
7223772DNAArtificial Sequencechemically synthesized 237atacgggagc
caacaccaac tgactcagtc tgctggtggg ctatattttt gcggagagca 60ggtgtgacgg
at
7223872DNAArtificial Sequencechemically synthesized 238atccgtcaca
cctgctctcc gcaaaaatat agcccaccag cagactgagt cagttggtgt 60tggctcccgt
at
7223972DNAArtificial Sequencechemically synthesized 239atacgggagc
caacaccaga ccgggcggta aaataacaaa ttacatcccc accgagagca 60ggtgtgacgg
at
7224072DNAArtificial Sequencechemically synthesized 240atccgtcaca
cctgctctcg gtggggatgt aatttgttat tttaccgccc ggtctggtgt 60tggctcccgt
at
7224172DNAArtificial Sequencechemically synthesized 241atacgggagc
caacaccaat gaccatatca aaccacaccg gccccccact gatgagagca 60ggtgtgacgg
at
7224272DNAArtificial Sequencechemically synthesized 242atccgtcaca
cctgctctca tcagtggggg gccggtgtgg tttgatatgg tcattggtgt 60tggctcccgt
at
7224372DNAArtificial Sequencechemically synthesized 243atacgggagc
caacaccaat gaccatatca aaccacaccg gccccccact gatgagagca 60ggtgtgacgg
at
7224472DNAArtificial Sequencechemically synthesized 244atccgtcaca
cctgctctca tcagtggggg gccggtgtgg tttgatatgg tcattggtgt 60tggctcccgt
at
7224572DNAArtificial Sequencechemically synthesized 245atacgggagc
caacaccact caagtccaga tcacactcgt gctgctaccg gttcagagca 60ggtgtgacgg
at
7224672DNAArtificial Sequencechemically synthesized 246atccgtcaca
cctgctctga accggtagca gcacgagtgt gatctggact tgagtggtgt 60tggctcccgt
at
7224772DNAArtificial Sequencechemically synthesized 247atacgggagc
caacaccatt tctaactaat cgagtcggta ccactgggcc cctgagagca 60ggtgtgacgg
at
7224872DNAArtificial Sequencechemically synthesized 248atccgtcaca
cctgctctca ggggcccagt ggtaccgact cgattagtta gaaatggtgt 60tggctcccgt
at
7224972DNAArtificial Sequencechemically synthesized 249atacgggagc
caacaccatg gctaggaaat aatgtccatc ggcacatcct cccgagagca 60ggtgtgacgg
at
7225072DNAArtificial Sequencechemically synthesized 250atccgtcaca
cctgctctcg ggaggatgtg ccgatggaca ttatttccta gccatggtgt 60tggctcccgt
at
7225172DNAArtificial Sequencechemically synthesized 251atacgggagc
caacaccaac cgtacaaaca agctagtgtg gttaatactg cataagagca 60ggtgtgacgg
at
7225272DNAArtificial Sequencechemically synthesized 252atccgtcaca
cctgctctta tgcagtatta accacactag cttgtttgta cggttggtgt 60tggctcccgt
at
7225372DNAArtificial Sequencechemically synthesized 253atacgggagc
caacaccaga ggctaccggt aaatgggata tcattgcgtt tgcaagagca 60ggtgtgacgg
at
7225472DNAArtificial Sequencechemically synthesized 254atccgtcaca
cctgctcttg caaacgcaat gatatcccat ttaccggtag cctctggtgt 60tggctcccgt
at
7225572DNAArtificial Sequencechemically synthesized 255atacgggagc
caacaccact caaagggtta ccaatccgtt ggacacagta ctgtagagca 60ggtgtgacgg
at
7225672DNAArtificial Sequencechemically synthesized 256atccgtcaca
cctgctctac agtactgtgt ccaacggatt ggtaaccctt tgagtggtgt 60tggctcccgt
at
7225772DNAArtificial Sequencechemically synthesized 257atacgggagc
caacaccatg aacatacaag aggtagacct ggtagtcgta ttctagagca 60ggtgtgacgg
at
7225872DNAArtificial Sequencechemically synthesized 258atccgtcaca
cctgctctag aatacgacta ccaggtctac ctcttgtatg ttcatggtgt 60tggctcccgt
at
7225972DNAArtificial Sequencechemically synthesized 259atacgggagc
caacaccatc tgagccgtaa ttggttattt tccagtgaag tgcgagagca 60ggtgtgacgg
at
7226072DNAArtificial Sequencechemically synthesized 260atccgtcaca
cctgctctcg cacttcactg gaaaataacc aattacggct cagatggtgt 60tggctcccgt
at
7226172DNAArtificial Sequencechemically synthesized 261atacgggagc
caacaccatg actacgtggg caagacatcc cgcgcgtcca tcccagagca 60ggtgtgacgg
at
7226272DNAArtificial Sequencechemically synthesized 262atccgtcaca
cctgctctgg gatggacgcg cgggatgtct tgcccacgta gtcatggtgt 60tggctcccgt
at
7226371DNAArtificial Sequencechemically synthesized 263atacgggagc
caacaccaac atggcgtggc aggtaatcta acctccctac ccaagagcag 60gtgtgacgga t
7126471DNAArtificial Sequencechemically synthesized 264atccgtcaca
cctgctcttg ggtagggagg ttagattacc tgccacgcca tgttggtgtt 60ggctcccgta t
7126572DNAArtificial Sequencechemically synthesized 265atacgggagc
caacaccaca cgacccaagg aattggaaaa acaccgacat tccaagagca 60ggtgtgacgg
at
7226672DNAArtificial Sequencechemically synthesized 266atccgtcaca
cctgctcttg gaatgtcggt gtttttccaa ttccttgggt cgtgtggtgt 60tggctcccgt
at
7226772DNAArtificial Sequencechemically synthesized 267atacgggagc
caacaccaag actgataacc ttacgtccag taggggcaag ctatagagca 60ggtgtgacgg
at
7226872DNAArtificial Sequencechemically synthesized 268atccgtcaca
cctgctctat agcttgcccc tactggacgt aaggttatca gtcttggtgt 60tggctcccgt
at
7226972DNAArtificial Sequencechemically synthesized 269atacgggagc
caacaccaat aaccgttttc ggccatgaga atactgtcac ttacagagca 60ggtgtgacgg
at
7227072DNAArtificial Sequencechemically synthesized 270atccgtcaca
cctgctctgt aagtgacagt attctcatgg ccgaaaacgg ttattggtgt 60tggctcccgt
at
7227172DNAArtificial Sequencechemically synthesized 271atacgggagc
caacaccaag acgaatagct cactacctcg tgaactatcc cctgagagca 60ggtgtgacgg
at
7227272DNAArtificial Sequencechemically synthesized 272atccgtcaca
cctgctctca ggggatagtt cacgaggtag tgagctattc gtcttggtgt 60tggctcccgt
at
7227372DNAArtificial Sequencechemically synthesized 273atacgggagc
caacaccata caggatctat tcccagaaga gttggcatat accaagagca 60ggtgtgacgg
at
7227472DNAArtificial Sequencechemically synthesized 274atccgtcaca
cctgctcttg gtatatgcca actcttctgg gaatagatcc tgtatggtgt 60tggctcccgt
at
7227572DNAArtificial Sequencechemically synthesized 275atacgggagc
caacaccatg gagaacattt ccatgttggc gaccatgggc ctcgagagca 60ggtgtgacgg
at
7227672DNAArtificial Sequencechemically synthesized 276atccgtcaca
cctgctctcg aggcccatgg tcgccaacat ggaaatgttc tccatggtgt 60tggctcccgt
at
7227772DNAArtificial Sequencechemically synthesized 277atacgggagc
caacaccagt acgaattata ctacagaggg cgtccgcgtt ggggagagca 60ggtgtgacgg
at
7227872DNAArtificial Sequencechemically synthesized 278atccgtcaca
cctgctctcc ccaacgcgga cgccctctgt agtataattc gtactggtgt 60tggctcccgt
at
7227972DNAArtificial Sequencechemically synthesized 279atacgggagc
caacaccacg caacctacct ctcacggctg ttcatgacgg acttagagca 60ggtgtgacgg
at
7228072DNAArtificial Sequencechemically synthesized 280atccgtcaca
cctgctctaa gtccgtcatg aacagccgtg agaggtaggt tgcgtggtgt 60tggctcccgt
at
7228172DNAArtificial Sequencechemically synthesized 281atacgggagc
caacaccatg ccaggcaagt cattgtgagt atagaatcac ctttagagca 60ggtgtgacgg
at
7228272DNAArtificial Sequencechemically synthesized 282atccgtcaca
cctgctctaa aggtgattct atactcacaa tgacttgcct ggcatggtgt 60tggctcccgt
at
7228372DNAArtificial Sequencechemically synthesized 283atacgggagc
caacaccagg accgtcgatg attgcaggtg cgtggtaaat acgcagagca 60ggtgtgacgg
at
7228472DNAArtificial Sequencechemically synthesized 284atccgtcaca
cctgctctgc gtatttacca cgcacctgca atcatcgacg gtcctggtgt 60tggctcccgt
at
7228572DNAArtificial Sequencechemically synthesized 285atacgggagc
caacaccatg ctacatcggt cagaagatcc gagcctgccc gcgtagagca 60ggtgtgacgg
at
7228672DNAArtificial Sequencechemically synthesized 286atccgtcaca
cctgctctac gcgggcaggc tcggatcttc tgaccgatgt agcatggtgt 60tggctcccgt
at
7228772DNAArtificial Sequencechemically synthesized 287atacgggagc
caacaccaac tgggaaacgt tgatcgtagc tgagtgatag tgctagagca 60ggtgtgacgg
at
7228872DNAArtificial Sequencechemically synthesized 288atccgtcaca
cctgctctag cactatcact cagctacgat caacgtttcc cagttggtgt 60tggctcccgt
at
7228972DNAArtificial Sequencechemically synthesized 289atacgggagc
caacaccaat gtttgaagca aagccgaggc atgtagaaac gtctagagca 60ggtgtgacgg
at
7229072DNAArtificial Sequencechemically synthesized 290atccgtcaca
cctgctctag acgtttctac atgcctcggc tttgcttcaa acattggtgt 60tggctcccgt
at
7229172DNAArtificial Sequencechemically synthesized 291atacgggagc
caacaccaga gggggtcaaa aatgctagag aatacaccgt cagcagagca 60ggtgtgacgg
at
7229272DNAArtificial Sequencechemically synthesized 292atccgtcaca
cctgctctgc tgacggtgta ttctctagca tttttgaccc cctctggtgt 60tggctcccgt
at
7229372DNAArtificial Sequencechemically synthesized 293atacgggagc
caacaccaaa gcgagctcgg catgtcgacg gtgtgcgtcc accgagagca 60ggtgtgacgg
at
7229472DNAArtificial Sequencechemically synthesized 294atccgtcaca
cctgctctcg gtggacgcac accgtcgaca tgccgagctc gctttggtgt 60tggctcccgt
at
7229572DNAArtificial Sequencechemically synthesized 295atacgggagc
caacaccatt cccccctctc gttctgttgc cccgtatcct aattagagca 60ggtgtgacgg
at
7229671DNAArtificial Sequencechemically synthesized 296atacgggagc
caacaccagc cggatatccg atgtgcttgt ctgacctggt ccgagagcag 60gtgtgacgga t
7129772DNAArtificial Sequencechemically synthesized 297atacgggagc
caacaccact gttatgttag ctcctcccta aactctttga ctcaagagca 60ggtgtgacgg
at
7229872DNAArtificial Sequencechemically synthesized 298atacgggagc
caacaccacc gcatgcgttc ccagcccgct gatgcattgt tgttagagca 60ggtgtgacgg
at
7229972DNAArtificial Sequencechemically synthesized 299atacgggagc
caacaccaga tcctgaaact atcactcctg cttataacct gttcagagca 60ggtgtgacgg
at
7230071DNAArtificial Sequencechemically synthesized 300atacgggagc
caacaccaac ccgggtgctg accactcggt ttgcgagccg ccgagagcag 60gtgtgacgga t
7130172DNAArtificial Sequencechemically synthesized 301atacgggagc
caacaccact atgttttcac ggtcttgtgg attacgtctt gcgcagagca 60ggtgtgacgg
at
7230270DNAArtificial Sequencechemically synthesized 302atacgggagc
caacaccaga gcaggtgtga tggatatacg ggagccaaca ccagagcagg 60tgtgacggat
7030370DNAArtificial Sequencechemically synthesized 303atacgggagc
caacaccaga gcaggtgtga tggatatacg ggagccaaca ccagagcagg 60tgtgacggat
7030470DNAArtificial Sequencechemically synthesized 304atacgggagc
caacaccaga gcaggtgtga tggatatacg ggagccaaca ccagagcagg 60tgtgacggat
7030572DNAArtificial Sequencechemically synthesized 305atccgtcaca
cctgctctgc acctgaagat caagtgtgag ttataggttg cgtttggtgt 60tggctcccgt
at
7230672DNAArtificial Sequencechemically synthesized 306atacgggagc
caacaccaat gcactcgtca cgcctttttt gctcttgcgc gttaagagca 60ggtgtgacgg
at
7230771DNAArtificial Sequencechemically synthesized 307atacgggagc
caacaccaac tggtctctac ggaaacctta cgccaatcta cacagagcag 60gtgtgacgga t
7130872DNAArtificial Sequencechemically synthesized 308atacgggagc
caacaccatt agcaacgatt cccctgttag ttccggtgca tcccagagca 60ggtgtgacgg
at
7230969DNAArtificial Sequencechemically synthesized 309atacgggagc
caacaccaga gcaggtgtga ggtcgaattc cagcacactg gcggccgtta 60ctagtggat
6931072DNAArtificial Sequencechemically synthesized 310atccgtcaca
cctgctctaa ttaggatacg gggcaacaga acgagagggg ggaatggtgt 60tggctcccgt
at
7231171DNAArtificial Sequencechemically synthesized 311atccgtcaca
cctgctctcg gaccaggtca gacaagcaca tcggatatcc ggctggtgtt 60ggctcccgta t
7131272DNAArtificial Sequencechemically synthesized 312atccgtcaca
cctgctcttg agtcaaagag tttagggagg agctaacata acagtggtgt 60tggctcccgt
at
7231372DNAArtificial Sequencechemically synthesized 313atccgtcaca
cctgctctaa caacaatgca tcagcgggct gggaacgcat gcggtggtgt 60tggctcccgt
at
7231472DNAArtificial Sequencechemically synthesized 314atccgtcaca
cctgctctga acaggttata agcaggagtg atagtttcag gatctggtgt 60tggctcccgt
at
7231571DNAArtificial Sequencechemically synthesized 315atccgtcaca
cctgctctcg gcggctcgca aaccgagtgg tcagcacccg ggttggtgtt 60ggctcccgta t
7131672DNAArtificial Sequencechemically synthesized 316atccgtcaca
cctgctctgc gcaagacgta atccacaaga ccgtgaaaac atagtggtgt 60tggctcccgt
at
7231770DNAArtificial Sequencechemically synthesized 317atccgtcaca
cctgctctgg tgttggctcc cgtatatcca tcacacctgc tctggtgttg 60gctcccgtat
7031870DNAArtificial Sequencechemically synthesized 318atccgtcaca
cctgctctgg tgttggctcc cgtatatcca tcacacctgc tctggtgttg 60gctcccgtat
7031970DNAArtificial Sequencechemically synthesized 319atccgtcaca
cctgctctgg tgttggctcc cgtatatcca tcacacctgc tctggtgttg 60gctcccgtat
7032072DNAArtificial Sequencechemically synthesized 320atccgtcaca
cctgctctgc acctgaagat caagtgtgag ttataggttg cgtttggtgt 60tggctcccgt
at
7232172DNAArtificial Sequencechemically synthesized 321atccgtcaca
cctgctctta acgcgcaaga gcaaaaaagg cgtgacgagt gcattggtgt 60tggctcccgt
at
7232271DNAArtificial Sequencechemically synthesized 322atccgtcaca
cctgctctgt gtagattggc gtaaggtttc cgtagagacc agttggtgtt 60ggctcccgta t
7132372DNAArtificial Sequencechemically synthesized 323atccgtcaca
cctgctctgg gatgcaccgg aactaacagg ggaatcgttg ctaatggtgt 60tggctcccgt
at
7232469DNAArtificial Sequencechemically synthesized 324atccactagt
aacggccgcc agtgtgctgg aattcgacct cacacctgct ctggtgttgg 60ctcccgtat
6932572DNAArtificial Sequencechemically synthesized 325atacgggagc
caacaccagg gggatggccg cttgacattg acacgtacct gtggagagca 60ggtgtgacgg
at
7232672DNAArtificial Sequencechemically synthesized 326atccgtcaca
cctgctctcc acaggtacgt gtcaatgtca agcggccatc cccctggtgt 60tggctcccgt
at
7232771DNAArtificial Sequencechemically synthesized 327atacgggagc
caacaccaca agaggccgta cgaaaaaata actatgcaat tgtagagcag 60gtgtgacgga t
7132871DNAArtificial Sequencechemically synthesized 328atccgtcaca
cctgctctac aattgcatag ttattttttc gtacggcctc ttgtggtgtt 60ggctcccgta t
7132972DNAArtificial Sequencechemically synthesized 329atacgggagc
caacaccaaa ccaaggaaga ataaaacgat cgagtgatca caccagagca 60ggtgtgacgg
at
7233072DNAArtificial Sequencechemically synthesized 330atccgtcaca
cctgctctgg tgtgatcact cgatcgtttt attcttcctt ggtttggtgt 60tggctcccgt
at
7233172DNAArtificial Sequencechemically synthesized 331atacgggagc
caacaccaca agcggagcta gctgggtccg tggatccaag catgagagca 60ggtgtgacgg
at
7233272DNAArtificial Sequencechemically synthesized 332atccgtcaca
cctgctctca tgcttggatc cacggaccca gctagctccg cttgtggtgt 60tggctcccgt
at
7233372DNAArtificial Sequencechemically synthesized 333atacgggagc
caacaccatg gcacgcagaa aacgttaaag cagaatttcc tcagagagca 60ggtgtgacgg
at
7233472DNAArtificial Sequencechemically synthesized 334atccgtcaca
cctgctctct gaggaaattc tgctttaacg ttttctgcgt gccatggtgt 60tggctcccgt
at
7233572DNAArtificial Sequencechemically synthesized 335atacgggagc
caacaccaga cttattctcg cagatccata aaaggataca ccccagagca 60ggtgtgacgg
at
7233672DNAArtificial Sequencechemically synthesized 336atccgtcaca
cctgctctgg ggtgtatcct tttatggatc tgcgagaata agtctggtgt 60tggctcccgt
at
7233772DNAArtificial Sequencechemically synthesized 337atacgggagc
caacaccaca gaatcacgtg gagtacgaac aattgattca cccaagagca 60ggtgtgacgg
at
7233872DNAArtificial Sequencechemically synthesized 338atccgtcaca
cctgctcttg ggtgaatcaa ttgttcgtac tccacgtgat tctgtggtgt 60tggctcccgt
at
7233972DNAArtificial Sequencechemically synthesized 339atacgggagc
caacaccacg ggggcatctt ccattaaccc attacctcac cccaagagca 60ggtgtgacgg
at
7234072DNAArtificial Sequencechemically synthesized 340atccgtcaca
cctgctcttg gggtgaggta atgggttaat ggaagatgcc cccgtggtgt 60tggctcccgt
at
7234172DNAArtificial Sequencechemically synthesized 341atacgggagc
caacaccaga aaaaaacaaa cccaaggaat tacaccacaa aagtagagca 60ggtgtgacgg
at
7234272DNAArtificial Sequencechemically synthesized 342atccgtcaca
cctgctctac ttttgtggtg taattccttg ggtttgtttt tttctggtgt 60tggctcccgt
at
7234371DNAArtificial Sequencechemically synthesized 343atacgggagc
caacaccata agcagagaac tacaagacga tcccatattc caaagagcag 60gtgtgacgga t
7134471DNAArtificial Sequencechemically synthesized 344atccgtcaca
cctgctcttt ggaatatggg atcgtcttgt agttctctgc ttatggtgtt 60ggctcccgta t
7134571DNAArtificial Sequencechemically synthesized 345atacgggagc
caacaccagg ttggcgtcaa aagaaataag ggatatacat tagagagcag 60gtgtgacgga t
7134671DNAArtificial Sequencechemically synthesized 346atccgtcaca
cctgctctct aatgtatatc ccttatttct tttgacgcca acctggtgtt 60ggctcccgta t
7134772DNAArtificial Sequencechemically synthesized 347atacgggagc
caacaccacg gggtccccat cgtggccgac ataacaaaaa tcctagagca 60ggtgtgacgg
at
7234872DNAArtificial Sequencechemically synthesized 348atccgtcaca
cctgctctag gatttttgtt atgtcggcca cgatggggac cccgtggtgt 60tggctcccgt
at
7234972DNAArtificial Sequencechemically synthesized 349atacgggagc
caacaccatg aggctcacag taagagaagg accttggact cgtcagagca 60ggtgtgacgg
at
7235072DNAArtificial Sequencechemically synthesized 350atccgtcaca
cctgctctga cgagtccaag gtccttctct tactgtgagc ctcatggtgt 60tggctcccgt
at
7235172DNAArtificial Sequencechemically synthesized 351atacgggagc
caacaccagc acgccctact ttaccgccga gaatatgaga atagagagca 60ggtgtgacgg
at
7235272DNAArtificial Sequencechemically synthesized 352atccgtcaca
cctgctctct attctcatat tctcggcggt aaagtagggc gtgctggtgt 60tggctcccgt
at
7235372DNAArtificial Sequencechemically synthesized 353atacgggagc
caacaccacc gatgcgggcg ccttcggcgg accgcgaggc ctcgagagca 60ggtgtgacgg
at
7235472DNAArtificial Sequencechemically synthesized 354atccgtcaca
cctgctctcg aggcctcgcg gtccgccgaa ggcgcccgca tcggtggtgt 60tggctcccgt
at
7235572DNAArtificial Sequencechemically synthesized 355atacgggagc
caacaccagg cgtaacgtat tgagcagaat aaataagaca cgatagagca 60ggtgtgacgg
at
7235672DNAArtificial Sequencechemically synthesized 356atccgtcaca
cctgctctat cgtgtcttat ttattctgct caatacgtta cgcctggtgt 60tggctcccgt
at
7235772DNAArtificial Sequencechemically synthesized 357atacgggagc
caacaccagg cggacgccga ccgggcgggc ccggtcgagg ttttagagca 60ggtgtgacgg
at
7235872DNAArtificial Sequencechemically synthesized 358atccgtcaca
cctgctctaa aacctcgacc gggcccgccc ggtcggcgtc cgcctggtgt 60tggctcccgt
at
7235972DNAArtificial Sequencechemically synthesized 359atacgggagc
caacaccagg cgggctcgcg cgtctcccag agccgagtca tatgagagca 60ggtgtgacgg
at
7236072DNAArtificial Sequencechemically synthesized 360atccgtcaca
cctgctctca tatgactcgg ctctgggaga cgcgcgagcc cgcctggtgt 60tggctcccgt
at
7236172DNAArtificial Sequencechemically synthesized 361atacgggagc
caacaccacg taccaatccc ctcggattaa ctcgaacaac ggtaagagca 60ggtgtgacgg
at
7236272DNAArtificial Sequencechemically synthesized 362atccgtcaca
cctgctctta ccgttgttcg agttaatccg aggggattgg tacgtggtgt 60tggctcccgt
at
7236372DNAArtificial Sequencechemically synthesized 363atacgggagc
caacaccact ggtgaggcgc ctgcgccgac tggccgtccc cccgagagca 60ggtgtgacgg
at
7236472DNAArtificial Sequencechemically synthesized 364atccgtcaca
cctgctctcg gggggacggc cagtcggcgc aggcgcctca ccagtggtgt 60tggctcccgt
at
7236571DNAArtificial Sequencechemically synthesized 365atacgggagc
caacaccagc agggtggcgc gaccgaccta tttgttggct attagagcag 60gtgtgacgga t
7136671DNAArtificial Sequencechemically synthesized 366atccgtcaca
cctgctctaa tagccaacaa ataggtcggt cgcgccaccc tgctggtgtt 60ggctcccgta t
7136772DNAArtificial Sequencechemically synthesized 367atacgggagc
caacaccact aacaagaaga acgacacgta cagcagtact taccagagca 60ggtgtgacgg
at
7236872DNAArtificial Sequencechemically synthesized 368atccgtcaca
cctgctctgg taagtactgc tgtacgtgtc gttcttcttg ttagtggtgt 60tggctcccgt
at
7236972DNAArtificial Sequencechemically synthesized 369atacgggagc
caacaccacc acgctagcgc accaaaatct ataaaccgaa tcctagagca 60ggtgtgacgg
at
7237072DNAArtificial Sequencechemically synthesized 370atccgtcaca
cctgctctag gattcggttt atagattttg gtgcgctagc gtggtggtgt 60tggctcccgt
at
7237162DNAArtificial Sequencechemically synthesized 371atacgggagc
caacaccaga gcaggtgtga cggatagagc aggtagagca ggtgtgacgg 60at
6237262DNAArtificial Sequencechemically synthesized 372atccgtcaca
cctgctctac ctgctctatc cgtcacacct gctctggtgt tggctcccgt 60at
6237372DNAArtificial Sequencechemically synthesized 373atacgggagc
caacaccacg aacccccggt cccccggggg actatattcc tacgagagca 60ggtgtgacgg
at
7237472DNAArtificial Sequencechemically synthesized 374atccgtcaca
cctgctctcg taggaatata gtcccccggg ggaccggggg ttcgtggtgt 60tggctcccgt
at
7237572DNAArtificial Sequencechemically synthesized 375atacgggagc
caacaccaac ggagagcttc tccgcatgtg aaccgttacc ttccagagca 60ggtgtgacgg
at
7237672DNAArtificial Sequencechemically synthesized 376atccgtcaca
cctgctctgg aaggtaacgg ttcacatgcg gagaagctct ccgttggtgt 60tggctcccgt
at
7237772DNAArtificial Sequencechemically synthesized 377atacgggagc
caacaccaca tgacgggtga ctaaaagacg aaatctccca attaagagca 60ggtgtgacgg
at
7237872DNAArtificial Sequencechemically synthesized 378atccgtcaca
cctgctctta attgggagat ttcgtctttt agtcacccgt catgtggtgt 60tggctcccgt
at
7237972DNAArtificial Sequencechemically synthesized 379atacgggagc
caacaccacc tgagcgccgg cagcactcac tatttgtccc cgcaagagca 60ggtgtgacgg
at
7238072DNAArtificial Sequencechemically synthesized 380atccgtcaca
cctgctcttg cggggacaaa tagtgagtgc tgccggcgct caggtggtgt 60tggctcccgt
at
7238172DNAArtificial Sequencechemically synthesized 381atacgggagc
caacaccaac atcctctgct gccggccgga ttcggccgag tgccagagca 60ggtgtgacgg
at
7238272DNAArtificial Sequencechemically synthesized 382atccgtcaca
cctgctctgg cactcggccg aatccggccg gcagcagagg atgttggtgt 60tggctcccgt
at
7238372DNAArtificial Sequencechemically synthesized 383atacgggagc
caacaccacg ccgccccagc gtcatcggaa gtctaatcag atccagagca 60ggtgtgacgg
at
7238472DNAArtificial Sequencechemically synthesized 384atccgtcaca
cctgctctgg atctgattag acttccgatg acgctggggc ggcgtggtgt 60tggctcccgt
at
7238572DNAArtificial Sequencechemically synthesized 385atacgggagc
caacaccagg gtggcgccgg ccagcgactc ctgccttcca agctagagca 60ggtgtgacgg
at
7238672DNAArtificial Sequencechemically synthesized 386atccgtcaca
cctgctctag cttggaaggc aggagtcgct ggccggcgcc accctggtgt 60tggctcccgt
at
7238772DNAArtificial Sequencechemically synthesized 387atacgggagc
caacaccaga cgggttggat cacgcctaat tgggcccatg ttttagagca 60ggtgtgacgg
at
7238872DNAArtificial Sequencechemically synthesized 388atccgtcaca
cctgctctaa aacatgggcc caattaggcg tgatccaacc cgtctggtgt 60tggctcccgt
at
7238972DNAArtificial Sequencechemically synthesized 389atacgggagc
caacaccact ctcaagtcga agatagacga aaagatttcg ccccagagca 60ggtgtgacgg
at
7239072DNAArtificial Sequencechemically synthesized 390atccgtcaca
cctgctctgg ggcgaaatct tttcgtctat cttcgacttg agagtggtgt 60tggctcccgt
at
7239172DNAArtificial Sequencechemically synthesized 391atacgggagc
caacaccatg cgagcatcat tacttcctac catggatccg gactagagca 60ggtgtgacgg
at
7239272DNAArtificial Sequencechemically synthesized 392atccgtcaca
cctgctctag tccggatcca tggtaggaag taatgatgct cgcatggtgt 60tggctcccgt
at
7239372DNAArtificial Sequencechemically synthesized 393atacgggagc
caacaccatc ggcgaccgtt agccttccgg ctacaatcag ggccagagca 60ggtgtgacgg
at
7239472DNAArtificial Sequencechemically synthesized 394atccgtcaca
cctgctctgg ccctgattgt agccggaagg ctaacggtcg ccgatggtgt 60tggctcccgt
at
7239570DNAArtificial Sequencechemically synthesized 395atacgggagc
caacaccaga gcgggtgtga cggatatacg ggagccaaca ccagagcagg 60tgtgacggat
7039670DNAArtificial Sequencechemically synthesized 396atccgtcaca
cctgctctgg tgttggctcc cgtatatccg tcacacccgc tctggtgttg 60gctcccgtat
7039770DNAArtificial Sequencechemically synthesized 397atacgggagc
caacaccacc gaattgattg ataaaaaggg ttagatcgct ccagagcagg 60tgtgacggat
7039870DNAArtificial Sequencechemically synthesized 398atccgtcaca
cctgctctgg agcgatctaa ccctttttat caatcaattc ggtggtgttg 60gctcccgtat
7039972DNAArtificial Sequencechemically synthesized 399atacgggagc
caacaccagc agggagcaat aaaaaccatt aaaagtcagc tgacagagca 60ggtgtgacgg
at
7240072DNAArtificial Sequencechemically synthesized 400atccgtcaca
cctgctctgt cagctgactt ttaatggttt ttattgctcc ctgctggtgt 60tggctcccgt
at
7240172DNAArtificial Sequencechemically synthesized 401atacgggagc
caacaccaca ggctcacgac cccggcttgg gccgggccct ccccagagca 60ggtgtgacgg
at
7240272DNAArtificial Sequencechemically synthesized 402atccgtcaca
cctgctctgg ggagggcccg gcccaagccg gggtcgtgag cctgtggtgt 60tggctcccgt
at
7240372DNAArtificial Sequencechemically synthesized 403atacgggagc
caacaccatc ccaagtcaat acctccgaaa taagttgaat taccagagca 60ggtgtgacgg
at
7240472DNAArtificial Sequencechemically synthesized 404atccgtcaca
cctgctctgg taattcaact tatttcggag gtattgactt gggatggtgt 60tggctcccgt
at
7240572DNAArtificial Sequencechemically synthesized 405atacgggagc
caacaccagt atctggatca agagaactga aggatatttc taacagagca 60ggtgtgacgg
at
7240672DNAArtificial Sequencechemically synthesized 406atccgtcaca
cctgctctgt tagaaatatc cttcagttct cttgatccag atactggtgt 60tggctcccgt
at
7240772DNAArtificial Sequencechemically synthesized 407atacgggagc
caacaccacg tgctgactat actattcaaa aacaacaccc taggagagca 60ggtgtgacgg
at
7240872DNAArtificial Sequencechemically synthesized 408atccgtcaca
cctgctctcc tagggtgttg tttttgaata gtatagtcag cacgtggtgt 60tggctcccgt
at
7240972DNAArtificial Sequencechemically synthesized 409atacgggagc
caacaccacg gataagaatc aataggcaat gaaagaagac cggcagagca 60ggtgtgacgg
at
7241072DNAArtificial Sequencechemically synthesized 410atccgtcaca
cctgctctgc cggtcttctt tcattgccta ttgattctta tccgtggtgt 60tggctcccgt
at
7241172DNAArtificial Sequencechemically synthesized 411atacgggagc
caacaccacg cgaagcgaga tccgccgtat tagcaatttg tgtgagagca 60ggtgtgacgg
at
7241272DNAArtificial Sequencechemically synthesized 412atccgtcaca
cctgctctca cacaaattgc taatacggcg gatctcgctt cgcgtggtgt 60tggctcccgt
at
7241372DNAArtificial Sequencechemically synthesized 413atacgggagc
caacaccacc gaccatggag cggacccatt agatcttcaa tcctagagca 60ggtgtgacgg
at
7241472DNAArtificial Sequencechemically synthesized 414atccgtcaca
cctgctctag gattgaagat ctaatgggtc cgctccatgg tcggtggtgt 60tggctcccgt
at
7241572DNAArtificial Sequencechemically synthesized 415atacgggagc
caacaccacc ccgaccagga gccggggctc ccgcccgcct ccccagagca 60ggtgtgacgg
at
7241672DNAArtificial Sequencechemically synthesized 416atccgtcaca
cctgctctgg ggaggcgggc gggagccccg gctcctggtc ggggtggtgt 60tggctcccgt
at
7241772DNAArtificial Sequencechemically synthesized 417atacgggagc
caacaccagg cgagcggtcc acggccggcc ggaccgtcaa tggtagagca 60ggtgtgacgg
at
7241872DNAArtificial Sequencechemically synthesized 418atccgtcaca
cctgctctac cattgacggt ccggccggcc gtggaccgct cgcctggtgt 60tggctcccgt
at
7241972DNAArtificial Sequencechemically synthesized 419atacgggagc
caacaccagg aggcggacga tctccctccc ggccaaaggc gcgtagagca 60ggtgtgacgg
at
7242072DNAArtificial Sequencechemically synthesized 420atccgtcaca
cctgctctac gcgcctttgg ccgggaggga gatcgtccgc ctcctggtgt 60tggctcccgt
at
7242172DNAArtificial Sequencechemically synthesized 421atacgggagc
caacaccacg gataagaatc aataggcaat gaaagaagac cggcagagca 60ggtgtgacgg
at
7242272DNAArtificial Sequencechemically synthesized 422atccgtcaca
cctgctctgc cggtcttctt tcattgccta ttgattctta tccgtggtgt 60tggctcccgt
at
7242372DNAArtificial Sequencechemically synthesized 423atacgggagc
caacaccact gtttaataaa tgcacctgaa aaaaacgtgt gtcaagagca 60ggtgtgacgg
at
7242472DNAArtificial Sequencechemically synthesized 424atccgtcaca
cctgctcttg acacacgttt ttttcaggtg catttattaa acagtggtgt 60tggctcccgt
at
7242571DNAArtificial Sequencechemically synthesized 425atacgggagc
caacaccaag cgtctagcgg gactaagctg aaacatacca gctagagcag 60gtgtgacgga t
7142671DNAArtificial Sequencechemically synthesized 426atccgtcaca
cctgctctag ctggtatgtt tcagcttagt cccgctagac gcttggtgtt 60ggctcccgta t
7142772DNAArtificial Sequencechemically synthesized 427atacgggagc
caacaccagg cgaccgacac gattataacc ttgtaaaggc cgtaagagca 60ggtgtgacgg
at
7242872DNAArtificial Sequencechemically synthesized 428atccgtcaca
cctgctctta cggcctttac aaggttataa tcgtgtcggt cgcctggtgt 60tggctcccgt
at
7242972DNAArtificial Sequencechemically synthesized 429atacgggagc
caacaccagt acggcggtgt ccgaactcac tatacccagt tgaaagagca 60ggtgtgacgg
at
7243072DNAArtificial Sequencechemically synthesized 430atccgtcaca
cctgctcttt caactgggta tagtgagttc ggacaccgcc gtactggtgt 60tggctcccgt
at
7243172DNAArtificial Sequencechemically synthesized 431atacgggagc
caacaccaga cctgacaacg aaaaccccag ttgtcgccat agccagagca 60ggtgtgacgg
at
7243272DNAArtificial Sequencechemically synthesized 432atccgtcaca
cctgctctgg ctatggcgac aactggggtt ttcgttgtca ggtctggtgt 60tggctcccgt
at
7243369DNAArtificial Sequencechemically synthesized 433atacgggagc
caacaccata gtgttgggcc aatacggtaa cgtgtccttg gagagcaggt 60gtgacggat
6943469DNAArtificial Sequencechemically synthesized 434atccgtcaca
cctgctctcc aaggacacgt taccgtattg gcccaacact atggtgttgg 60ctcccgtat
6943572DNAArtificial Sequencechemically synthesized 435atacgggagc
caacaccata agcgcaacac agtccatccc tgagtgagat agcgagagca 60ggtgtgacgg
at
7243672DNAArtificial Sequencechemically synthesized 436atccgtcaca
cctgctctcg ctatctcact cagggatgga ctgtgttgcg cttatggtgt 60tggctcccgt
at
7243758DNAArtificial Sequencechemically synthesized 437atacgggagc
caacaccacg cacatactag ctatctcatc agagcaggtg tgacggat
5843858DNAArtificial Sequencechemically synthesized 438atccgtcaca
cctgctctga tgagatagct agtatgtgcg tggtgttggc tcccgtat
5843958DNAArtificial Sequencechemically synthesized 439atacgggagc
caacaccact aacttgttgc tgatcttacc agagcaggtg tgacggat
5844058DNAArtificial Sequencechemically synthesized 440atccgtcaca
cctgctctgg taagatcagc aacaagttag tggtgttggc tcccgtat
5844172DNAArtificial Sequencechemically synthesized 441atacgggagc
caacaccacc cgtttttgat ctaatgagga tacaatattc gtcnagagca 60ggtgtgacgg
at
7244272DNAArtificial Sequencechemically synthesized 442atccgtcaca
cctgctctng acgaatattg tatcctcatt agatcaaaaa cgggtggtgt 60tggctcccgt
at
7244372DNAArtificial Sequencechemically synthesized 443atacgggagc
caacaccagt tgtgggaaca tcaggctaag tatgagacgg aacgagagca 60ggtgtgacgg
at
7244472DNAArtificial Sequencechemically synthesized 444atccgtcaca
cctgctctcg ttccgtctca tacttagcct gatgttccca caactggtgt 60tggctcccgt
at
7244572DNAArtificial Sequencechemically synthesized 445atacgggagc
caacaccata gacaatggcg tacttttcgt aattccacaa gaatagagca 60ggtgtgacgg
at
7244672DNAArtificial Sequencechemically synthesized 446atccgtcaca
cctgctctat tcttgtggaa ttacgaaaag tacgccattg tctatggtgt 60tggctcccgt
at
7244769DNAArtificial Sequencechemically synthesized 447atacgggagc
caacaccata gtgttgggcc aatacggtaa cgtgtccttg gagagcaggt 60gtgacggat
6944869DNAArtificial Sequencechemically synthesized 448atccgtcaca
cctgctctcc aaggacacgt taccgtattg gcccaacact atggtgttgg 60ctcccgtat
6944958DNAArtificial Sequencechemically synthesized 449atacgggagc
caacaccacc acaaaagcat tcgcccttac agagcaggtg tgacggat
5845058DNAArtificial Sequencechemically synthesized 450atccgtcaca
cctgctctgt aagggcgaat gcttttgtgg tggtgttggc tcccgtat
5845172DNAArtificial Sequencechemically synthesized 451atacgggagc
caacaccagc gtgtagctag tttcaggatt gtagtatgta atatagagca 60ggtgtgacgg
at
7245272DNAArtificial Sequencechemically synthesized 452atccgtcaca
cctgctctat attacatact acaatcctga aactagctac acgctggtgt 60tggctcccgt
at
7245358DNAArtificial Sequencechemically synthesized 453atacgggagc
caacaccacg cacatactag ctatctcatc agagcaggtg tgacggat
5845458DNAArtificial Sequencechemically synthesized 454atccgtcaca
cctgctctga tgagatagct agtatgtgcg tggtgttggc tcccgtat
5845572DNAArtificial Sequencechemically synthesized 455atacgggagc
caacaccatc agagatcatc taacgaaaat catgggtctc gcccagagca 60ggtgtgacgg
at
7245672DNAArtificial Sequencechemically synthesized 456atccgtcaca
cctgctctgg gcgagaccca tgattttcgt tagatgatct ctgatggtgt 60tggctcccgt
at
7245772DNAArtificial Sequencechemically synthesized 457atacgggagc
caacaccagc aaagaatagt gagccctatg atcatctgtt cgtcagagca 60ggtgtgacgg
at
7245872DNAArtificial Sequencechemically synthesized 458atccgtcaca
cctgctctga cgaacagatg atcatagggc tcactattct ttgctggtgt 60tggctcccgt
at
7245972DNAArtificial Sequencechemically synthesized 459atacgggagc
caacaccaga catcatgtcg catatctgga tctagaggct attcagagca 60ggtgtgacgg
at
7246072DNAArtificial Sequencechemically synthesized 460atccgtcaca
cctgctctga atagcctcta gatccagata tgcgacatga tgtctggtgt 60tggctcccgt
at
7246169DNAArtificial Sequencechemically synthesized 461atacgggagc
caacaccata gtgttgggcc aatacggtaa cgtgtacttg gagagcaggt 60gtgacggat
6946269DNAArtificial Sequencechemically synthesized 462atccgtcaca
cctgctctcc aagtacacgt taccgtattg gcccaacact atggtgttgg 60ctcccgtat
6946372DNAArtificial Sequencechemically synthesized 463atacgggagc
caacaccaga gagttcatgt tgcacccggg tcgtcgcgtt atgcagagca 60ggtgtgacgg
at
7246472DNAArtificial Sequencechemically synthesized 464atccgtcaca
cctgctctgc ataacgcgac gacccgggtg caacatgaac tctctggtgt 60tggctcccgt
at
7246572DNAArtificial Sequencechemically synthesized 465atacgggagc
caacaccatc tatctgaggc ctatttagca cctgttgtca ccctagagca 60ggtgtgacgg
at
7246672DNAArtificial Sequencechemically synthesized 466atccgtcaca
cctgctctag ggtgacaaca ggtgctaaat aggcctcaga tagatggtgt 60tggctcccgt
at
7246772DNAArtificial Sequencechemically synthesized 467atacgggagc
caacaccatt ctatcgttcc ggacgcttat gccttgccat ctacagagca 60ggtgtgacgg
at
7246872DNAArtificial Sequencechemically synthesized 468atccgtcaca
cctgctctgt agatggcaag gcataagcgt ccggaacgat agaatggtgt 60tggctcccgt
at
7246972DNAArtificial Sequencechemically synthesized 469atacgggagc
caacaccaca tttcgcacac ccgagcccta gagggctagg gaccagagca 60ggtgtgacgg
at
7247072DNAArtificial Sequencechemically synthesized 470atccgtcaca
cctgctctgg tccctagccc tctagggctc gggtgtgcga aatgtggtgt 60tggctcccgt
at
7247172DNAArtificial Sequencechemically synthesized 471atacgggagc
caacaccact ggcacgaaca atcatgaaat agggcatccg agagagagca 60ggtgtgacgg
at
7247272DNAArtificial Sequencechemically synthesized 472atccgtcaca
cctgctctct ctcggatgcc ctatttcatg attgttcgtg ccagtggtgt 60tggctcccgt
at
7247372DNAArtificial Sequencechemically synthesized 473atacgggagc
caacaccatg tgtcatggtg ggcgtggcta tccgagcaag ctctagagca 60ggtgtgacgg
at
7247472DNAArtificial Sequencechemically synthesized 474atccgtcaca
cctgctctag agcttgctcg gatagccacg cccaccatga cacatggtgt 60tggctcccgt
at
7247572DNAArtificial Sequencechemically synthesized 475atacgggagc
caacaccaca tgcaaataca catcatgatg acggatccta ttgtagagca 60ggtgtgacgg
at
7247672DNAArtificial Sequencechemically synthesized 476atccgtcaca
cctgctctac aataggatcc gtcatcatga tgtgtatttg catgtggtgt 60tggctcccgt
at
7247772DNAArtificial Sequencechemically synthesized 477atacgggagc
caacaccagt agagcaagct attacgcgaa gctagaatga gatcagagca 60ggtgtgacgg
at
7247872DNAArtificial Sequencechemically synthesized 478atccgtcaca
cctgctctga tctcattcta gcttcgcgta atagcttgct ctactggtgt 60tggctcccgt
at
7247972DNAArtificial Sequencechemically synthesized 479atacgggagc
caacaccacc cattggggat gcattagatg cagttgatta tgcgagagca 60ggtgtgacgg
at
7248072DNAArtificial Sequencechemically synthesized 480atccgtcaca
cctgctctcg cataatcaac tgcatctaat gcatccccaa tgggtggtgt 60tggctcccgt
at
7248172DNAArtificial Sequencechemically synthesized 481atacgggagc
caacaccata caaccgtcca agcgttactc tttacatact ctccagagca 60ggtgtgacgg
at
7248272DNAArtificial Sequencechemically synthesized 482atccgtcaca
cctgctctgg agagtatgta aagagtaacg cttggacggt tgtatggtgt 60tggctcccgt
at
7248372DNAArtificial Sequencechemically synthesized 483atacgggagc
caacaccaat tttgactgtt ccaggctcag tattggtatt caggagagca 60ggtgtgacgg
at
7248472DNAArtificial Sequencechemically synthesized 484atccgtcaca
cctgctctcc tgaataccaa tactgagcct ggaacagtca aaattggtgt 60tggctcccgt
at
7248572DNAArtificial Sequencechemically synthesized 485atacgggagc
caacaccacg aaatgatgta tctagttcct cgtgtcctga ttcgagagca 60ggtgtgacgg
at
7248672DNAArtificial Sequencechemically synthesized 486atccgtcaca
cctgctctcg aatcaggaca cgaggaacta gatacatcat ttcgtggtgt 60tggctcccgt
at
7248772DNAArtificial Sequencechemically synthesized 487atacgggagc
caacaccacc gctgcccagg tataacaccg cggtacgatc tcatagagca 60ggtgtgacgg
at
7248872DNAArtificial Sequencechemically synthesized 488atccgtcaca
cctgctctat gagatcgtac cgcggtgtta tacctgggca gcggtggtgt 60tggctcccgt
at
7248972DNAArtificial Sequencechemically synthesized 489atacgggagc
caacaccaaa gggatctgga aaaccccgta ttgatctaag gcgcagagca 60ggtgtgacgg
at
7249072DNAArtificial Sequencechemically synthesized 490atccgtcaca
cctgctctgc gccttagatc aatacggggt tttccagatc cctttggtgt 60tggctcccgt
at
7249172DNAArtificial Sequencechemically synthesized 491atacgggagc
caacaccata cccccctctt taggccatca cgttctgtcg atttagagca 60ggtgtgacgg
at
7249272DNAArtificial Sequencechemically synthesized 492atccgtcaca
cctgctctaa atcgacagaa cgtgatggcc taaagagggg ggtatggtgt 60tggctcccgt
at
7249372DNAArtificial Sequencechemically synthesized 493atacgggagc
caacaccata cccccctctt taggccatca cgttctctcg atttagagca 60ggtgtgacgg
at
7249472DNAArtificial Sequencechemically synthesized 494atccgtcaca
cctgctctaa atcgagagaa cgtgatggcc taaagagggg ggtatggtgt 60tggctcccgt
at
7249572DNAArtificial Sequencechemically synthesized 495atacgggagc
caacaccatc atggtcgcga atcaaccgta tctcagctgc cttgagagca 60ggtgtgacgg
at
7249672DNAArtificial Sequencechemically synthesized 496atccgtcaca
cctgctctca aggcagctga gatacggttg attcgcgacc atgatggtgt 60tggctcccgt
at
7249772DNAArtificial Sequencechemically synthesized 497atacgggagc
caacaccacc cgtttttgat ctaatgagga tacaatattc gtctagagca 60ggtgtgacgg
at
7249872DNAArtificial Sequencechemically synthesized 498atccgtcaca
cctgctctag acgaatattg tatcctcatt agatcaaaaa cgggtggtgt 60tggctcccgt
at
7249972DNAArtificial Sequencechemically synthesized 499atacgggagc
caacaccata taaaaggcga acggtcactt tggtcggagt acgtagagca 60ggtgtgacgg
at
7250072DNAArtificial Sequencechemically synthesized 500atccgtcaca
cctgctctac gtactccgac caaagtgacc gttcgccttt tatatggtgt 60tggctcccgt
at
7250157DNAArtificial Sequencechemically synthesized 501atacgggagc
caacaccagt gttggcgtct tccctgatca gagcaggtgt gacggat
5750257DNAArtificial Sequencechemically synthesized 502atccgtcaca
cctgctctga tcagggaaga cgccaacact ggtgttggct cccgtat
5750372DNAArtificial Sequencechemically synthesized 503atacgggagc
caacaccaga ggtgagaagc ccattacacc gagcggacct gcagagagca 60ggtgtgacgg
at
7250472DNAArtificial Sequencechemically synthesized 504atccgtcaca
cctgctctct gcaggtccgc tcggtgtaat gggcttctca cctctggtgt 60tggctcccgt
at
7250572DNAArtificial Sequencechemically synthesized 505atacgggagc
caacaccaga cgaaatcaaa tctaaaagtg ataagccaga agtcagagca 60ggtgtgacgg
at
7250672DNAArtificial Sequencechemically synthesized 506atccgtcaca
cctgctctga cttctggctt atcactttta gatttgattt cgtctggtgt 60tggctcccgt
at
7250772DNAArtificial Sequencechemically synthesized 507atacgggagc
caacaccacc cgtttttgat ctaatgagga tacaatattc gtctagagca 60ggtgtgacgg
at
7250872DNAArtificial Sequencechemically synthesized 508atccgtcaca
cctgctctag acgaatattg tatcctcatt agatcaaaaa cgggtggtgt 60tggctcccgt
at
7250972DNAArtificial Sequencechemically synthesized 509atacgggagc
caacaccaac acgcagaaga gatgcattgt atgatcggtg tacgagagca 60ggtgtgacgg
at
7251072DNAArtificial Sequencechemically synthesized 510atccgtcaca
cctgctctcg tacaccgatc atacaatgca tctcttctgc gtgttggtgt 60tggctcccgt
at
7251172DNAArtificial Sequencechemically synthesized 511atacgggagc
caacaccacg ccaacagtgt tttagagtca tgcacaaaag tatcagagca 60ggtgtgacgg
at
7251272DNAArtificial Sequencechemically synthesized 512atccgtcaca
cctgctctga tacttttgtg catgactcta aaacactgtt ggcgtggtgt 60tggctcccgt
at
7251372DNAArtificial Sequencechemically synthesized 513atacgggagc
caacaccagg gtggtttacg atcggcaggt cgtgcgtgcg acacagagca 60ggtgtgacgg
at
7251472DNAArtificial Sequencechemically synthesized 514atccgtcaca
cctgctctgt gtcgcacgca cgacctgccg atcgtaaacc accctggtgt 60tggctcccgt
at
7251572DNAArtificial Sequencechemically synthesized 515atacgggagc
caacaccaac ggcgacgggc gagtacacag gtgtaagtcg gggtagagca 60ggtgtgacgg
at
7251672DNAArtificial Sequencechemically synthesized 516atccgtcaca
cctgctctac cccgacttac acctgtgtac tcgcccgtcg ccgttggtgt 60tggctcccgt
at
7251772DNAArtificial Sequencechemically synthesized 517atacgggagc
caacaccaag caatagaaca ccctttgtcg cactggatgc gatgagagca 60ggtgtgacgg
at
7251872DNAArtificial Sequencechemically synthesized 518atccgtcaca
cctgctctca tcgcatccag tgcgacaaag ggtgttctat tgcttggtgt 60tggctcccgt
at
7251972DNAArtificial Sequencechemically synthesized 519atacgggagc
caacaccaca aagtgttgta agggcaaaac aaatatgtac ctcgagagca 60ggtgtgacgg
at
7252072DNAArtificial Sequencechemically synthesized 520atccgtcaca
cctgctctcg aggtacatat ttgttttgcc cttacaacac tttgtggtgt 60tggctcccgt
at
7252172DNAArtificial Sequencechemically synthesized 521atacgggagc
caacaccaag ccaaagaccc ttatcaaacg gccatgcccg gggcagagca 60ggtgtgacgg
at
7252272DNAArtificial Sequencechemically synthesized 522atccgtcaca
cctgctctgc cccgggcatg gccgtttgat aagggtcttt ggcttggtgt 60tggctcccgt
at
7252372DNAArtificial Sequencechemically synthesized 523atacgggagc
caacaccaca ttaaccccaa aggatacatg tttgtcccat cgcaagagca 60ggtgtgacgg
at
7252472DNAArtificial Sequencechemically synthesized 524atccgtcaca
cctgctcttg cgatgggaca aacatgtatc ctttggggtt aatgtggtgt 60tggctcccgt
at
7252572DNAArtificial Sequencechemically synthesized 525atacgggagc
caacaccata tcacggaccc cccaggttgc cgaattactc ttacagagca 60ggtgtgacgg
at
7252672DNAArtificial Sequencechemically synthesized 526atccgtcaca
cctgctctgt aagagtaatt cggcaacctg gggggtccgt gatatggtgt 60tggctcccgt
at
7252770DNAArtificial Sequencechemically synthesized 527atacgggagc
caacaccatt cccgcatcgc gcgttttcag cctttgaccg ttagagcagg 60tgtgacggat
7052870DNAArtificial Sequencechemically synthesized 528atccgtcaca
cctgctctaa cggtcaaagg ctgaaaacgc gcgatgcggg aatggtgttg 60gctcccgtat
7052972DNAArtificial Sequencechemically synthesized 529atacgggagc
caacaccaac gtgtgctgtg ttactgccct tctctgtagc cgtgagagca 60ggtgtgacgg
at
7253072DNAArtificial Sequencechemically synthesized 530atccgtcaca
cctgctctca cggctacaga gaagggcagt aacacagcac acgttggtgt 60tggctcccgt
at
7253172DNAArtificial Sequencechemically synthesized 531atacgggagc
caacaccacc ttttaaaacg ctagccagct tagtccattc caccagagca 60ggtgtgacgg
at
7253272DNAArtificial Sequencechemically synthesized 532atccgtcaca
cctgctctgg tggaatggac taagctggct agcgttttaa aaggtggtgt 60tggctcccgt
at
7253372DNAArtificial Sequencechemically synthesized 533atacgggagc
caacaccaat ctaacagatt gcagctcgcc tgtcccggcg tactagagca 60ggtgtgacgg
at
7253472DNAArtificial Sequencechemically synthesized 534atccgtcaca
cctgctctag tacgccggga caggcgagct gcaatctgtt agattggtgt 60tggctcccgt
at
7253572DNAArtificial Sequencechemically synthesized 535atacgggagc
caacaccacc tattacagac ccaatttcca cctggcattt ctatagagca 60ggtgtgacgg
at
7253672DNAArtificial Sequencechemically synthesized 536atccgtcaca
cctgctctat agaaatgcca ggtggaaatt gggtctgtaa taggtggtgt 60tggctcccgt
at
7253772DNAArtificial Sequencechemically synthesized 537atacgggagc
caacaccaca tatcctacac tcccataccc cactgtagac acgcagagca 60ggtgtgacgg
at
7253872DNAArtificial Sequencechemically synthesized 538atccgtcaca
cctgctctgc gtgtctacag tggggtatgg gagtgtagga tatgtggtgt 60tggctcccgt
at
7253972DNAArtificial Sequencechemically synthesized 539atacgggagc
caacaccaaa ccgagtgctg gtggccctct ctgccatata agtgagagca 60ggtgtgacgg
at
7254072DNAArtificial Sequencechemically synthesized 540atccgtcaca
cctgctctca cttatatggc agagagggcc accagcactc ggtttggtgt 60tggctcccgt
at
7254173DNAArtificial Sequencechemically synthesized 541atacgggagc
caacaccacc ttttaaaacg ctagctagct tagtccaatt ccaccagagc 60aggtgtgacg
gat
7354273DNAArtificial Sequencechemically synthesized 542atccgtcaca
cctgctctgg tggaattgga ctaagctagc tagcgtttta aaaggtggtg 60ttggctcccg
tat
7354372DNAArtificial Sequencechemically synthesized 543atacgggagc
caacaccatg acattgcaac tatacgctta cccacgtcag ctccagagca 60ggtgtgacgg
at
7254472DNAArtificial Sequencechemically synthesized 544atccgtcaca
cctgctctgg agctgacgtg ggtaagcgta tagttgcaat gtcatggtgt 60tggctcccgt
at
7254572DNAArtificial Sequencechemically synthesized 545atacgggagc
caacaccatt aacctgaaag taccagtgtc agtttaccct acctagagca 60ggtgtgacgg
at
7254672DNAArtificial Sequencechemically synthesized 546atccgtcaca
cctgctctag gtagggtaaa ctgacactgg tactttcagg ttaatggtgt 60tggctcccgt
at
7254772DNAArtificial Sequencechemically synthesized 547atacgggagc
caacaccagg cattagtgta aagcactaag agtcaggctg tagcagagca 60ggtgtgacgg
at
7254872DNAArtificial Sequencechemically synthesized 548atccgtcaca
cctgctctgc tacagcctga ctcttagtgc tttacactaa tgcctggtgt 60tggctcccgt
at
7254972DNAArtificial Sequencechemically synthesized 549atacgggagc
caacaccatg acacgccgat tatggacgtt gcgaactagt tggtagagca 60ggtgtgacgg
at
7255072DNAArtificial Sequencechemically synthesized 550atccgtcaca
cctgctctac caactagttc gcaacgtcca taatcggcgt gtcatggtgt 60tggctcccgt
at
7255172DNAArtificial Sequencechemically synthesized 551atacgggagc
caacaccact caatcccacc cttatttaga gcggttacat cacaagagca 60ggtgtgacgg
at
7255272DNAArtificial Sequencechemically synthesized 552atccgtcaca
cctgctcttg tgatgtaacc gctctaaata agggtgggat tgagtggtgt 60tggctcccgt
at
7255372DNAArtificial Sequencechemically synthesized 553atacgggagc
caacaccaat ttgtgaaaat attcccgtgt tttccttgag cagcagagca 60ggtgtgacgg
at
7255472DNAArtificial Sequencechemically synthesized 554atccgtcaca
cctgctctgc tgctcaagga aaacacggga atattttcac aaattggtgt 60tggctcccgt
at
7255572DNAArtificial Sequencechemically synthesized 555atacgggagc
caacaccagc gtaaaacctt gtaccaattg atgacactag cggtagagca 60ggtgtgacgg
at
7255672DNAArtificial Sequencechemically synthesized 556atccgtcaca
cctgctctac cgctagtgtc atcaattggt acaaggtttt acgctggtgt 60tggctcccgt
at
7255772DNAArtificial Sequencechemically synthesized 557atacgggagc
caacaccaca gctcaccgcg cttgccgtgc cttacgtctg tccaagagca 60ggtgtgacgg
at
7255872DNAArtificial Sequencechemically synthesized 558atccgtcaca
cctgctcttg gacagacgta aggcacggca agcgcggtga gctgtggtgt 60tggctcccgt
at
7255971DNAArtificial Sequencechemically synthesized 559atacgggagc
caacaccatc accgtactgg agccatcgtt catccagcaa tctagagcag 60gtgtgacgga t
7156071DNAArtificial Sequencechemically synthesized 560atccgtcaca
cctgctctag attgctggat gaacgatggc tccagtacgg tgatggtgtt 60ggctcccgta t
7156172DNAArtificial Sequencechemically synthesized 561atacgggagc
caacaccacc tctcacatta tattgtgaat acttcgtgct gtttagagca 60ggtgtgacgg
at
7256272DNAArtificial Sequencechemically synthesized 562atccgtcaca
cctgctctaa acagcacgaa gtattcacaa tataatgtga gaggtggtgt 60tggctcccgt
at
7256372DNAArtificial Sequencechemically synthesized 563atacgggagc
caacaccagt gcttctgcct tccttatgtt ctatctgatt atctagagca 60ggtgtgacgg
at
7256472DNAArtificial Sequencechemically synthesized 564atccgtcaca
cctgctctag ataatcagat agaacataag gaaggcagaa gcactggtgt 60tggctcccgt
at
7256572DNAArtificial Sequencechemically synthesized 565atacgggagc
caacaccaaa attaacgtgt tgagatatgt ggaacctcta ctaaagagca 60ggtgtgacgg
at
7256672DNAArtificial Sequencechemically synthesized 566atccgtcaca
cctgctcttt agtagaggtt ccacatatct caacacgtta attttggtgt 60tggctcccgt
at
7256772DNAArtificial Sequencechemically synthesized 567atacgggagc
caacaccagt ccccattccc gtctgaatga ctgtccataa caggagagca 60ggtgtgacgg
at
7256872DNAArtificial Sequencechemically synthesized 568atccgtcaca
cctgctctcc tgttatggac agtcattcag acgggaatgg ggactggtgt 60tggctcccgt
at
7256972DNAArtificial Sequencechemically synthesized 569atacgggagc
caacaccacc tgaatactaa cactaagacc cgacttgtgt tcacagagca 60ggtgtgacgg
at
7257072DNAArtificial Sequencechemically synthesized 570atccgtcaca
cctgctctgt gaacacaagt cgggtcttag tgttagtatt caggtggtgt 60tggctcccgt
at
7257172DNAArtificial Sequencechemically synthesized 571atacgggagc
caacaccagc taggatgtca ccaccggtca cttcatgtcg agacagagca 60ggtgtgacgg
at
7257272DNAArtificial Sequencechemically synthesized 572atccgtcaca
cctgctctgt ctcgacatga agtgaccggt ggtgacatcc tagctggtgt 60tggctcccgt
at
7257372DNAArtificial Sequencechemically synthesized 573atacgggagc
caacaccata actattggcc tcgagccgct ccagtcatca tcctagagca 60ggtgtgacgg
at
7257472DNAArtificial Sequencechemically synthesized 574atccgtcaca
cctgctctag gatgatgact ggagcggctc gaggccaata gttatggtgt 60tggctcccgt
at
7257572DNAArtificial Sequencechemically synthesized 575atacgggagc
caacaccaga gcttgctttt cttacgtcgt tgccgggagc cggaagagca 60ggtgtgacgg
at
7257672DNAArtificial Sequencechemically synthesized 576atccgtcaca
cctgctcttc cggctcccgg caacgacgta agaaaagcaa gctctggtgt 60tggctcccgt
at
7257772DNAArtificial Sequencechemically synthesized 577atacgggagc
caacaccacc ggggacacta tacattctct agacaatata gtttagagca 60ggtgtgacgg
at
7257872DNAArtificial Sequencechemically synthesized 578atccgtcaca
cctgctctaa actatattgt ctagagaatg tatagtgtcc ccggtggtgt 60tggctcccgt
at
7257972DNAArtificial Sequencechemically synthesized 579atacgggagc
caacaccaga tagttgtcct gcatatgttg tcgtgtgcta atgtagagca 60ggtgtgacgg
at
7258072DNAArtificial Sequencechemically synthesized 580atccgtcaca
cctgctctac attagcacac gacaacatat gcaggacaac tatctggtgt 60tggctcccgt
at
7258172DNAArtificial Sequencechemically synthesized 581atacgggagc
caacaccaac tctacagcct aatctgcact ttacaggata cgtcagagca 60ggtgtgacgg
at
7258272DNAArtificial Sequencechemically synthesized 582atccgtcaca
cctgctctga cgtatcctgt aaagtgcaga ttaggctgta gagttggtgt 60tggctcccgt
at
7258372DNAArtificial Sequencechemically synthesized 583atacgggagc
caacaccacg cacgtggtct acacggccac ctattctcat ttgtagagca 60ggtgtgacgg
at
7258472DNAArtificial Sequencechemically synthesized 584atccgtcaca
cctgctctac aaatgagaat aggtggccgt gtagaccacg tgcgtggtgt 60tggctcccgt
at
7258572DNAArtificial Sequencechemically synthesized 585atacgggagc
caacaccatc ctctgttctt cacgcgggag cactttctat ctttagagca 60ggtgtgacgg
at
7258672DNAArtificial Sequencechemically synthesized 586atccgtcaca
cctgctctaa agatagaaag tgctcccgcg tgaagaacag aggatggtgt 60tggctcccgt
at
7258772DNAArtificial Sequencechemically synthesized 587atacgggagc
caacaccatc aatggcaagt tgttccatat tgaccatggt tcacagagca 60ggtgtgacgg
at
7258872DNAArtificial Sequencechemically synthesized 588atccgtcaca
cctgctctgt gaaccatggt caatatggaa caacttgcca ttgatggtgt 60tggctcccgt
at
7258972DNAArtificial Sequencechemically synthesized 589atacgggagc
caacaccaca ctaccgtccc accccctccc agctcctccg gccgagagca 60ggtgtgacgg
at
7259072DNAArtificial Sequencechemically synthesized 590atccgtcaca
cctgctctcg gccggaggag ctgggagggg gtgggacggt agtgtggtgt 60tggctcccgt
at
7259172DNAArtificial Sequencechemically synthesized 591atacgggagc
caacaccact caagtagtcc catacaccac gcaagcctct ctctagagca 60ggtgtgacgg
at
7259272DNAArtificial Sequencechemically synthesized 592atccgtcaca
cctgctctag agagaggctt gcgtggtgta tgggactact tgagtggtgt 60tggctcccgt
at
7259372DNAArtificial Sequencechemically synthesized 593atacgggagc
caacaccaat cttactagtt tgggaaaaaa attaaatata agcaagagca 60ggtgtgacgg
at
7259472DNAArtificial Sequencechemically synthesized 594atccgtcaca
cctgctcttg cttatattta atttttttcc caaactagta agattggtgt 60tggctcccgt
at
7259572DNAArtificial Sequencechemically synthesized 595atacgggagc
caacaccaca ctaccgtccc accccctccc agctcctccg gccgagagca 60ggtgtgacgg
at
7259672DNAArtificial Sequencechemically synthesized 596atccgtcaca
cctgctctcg gccggaggag ctgggagggg gtgggacggt agtgtggtgt 60tggctcccgt
at
7259772DNAArtificial Sequencechemically synthesized 597atacgggagc
caacaccagc ctagctcgct cccaaaagag tccacgcccc ggatagagca 60ggtgtgacgg
at
7259872DNAArtificial Sequencechemically synthesized 598atccgtcaca
cctgctctat ccggggcgtg gactcttttg ggagcgagct aggctggtgt 60tggctcccgt
at
7259972DNAArtificial Sequencechemically synthesized 599atacgggagc
caacaccaac aaatttccag cgctatgggc cctaattacc actaagagca 60ggtgtgacgg
at
7260072DNAArtificial Sequencechemically synthesized 600atccgtcaca
cctgctctta gtggtaatta gggcccatag cgctggaaat ttgttggtgt 60tggctcccgt
at
7260172DNAArtificial Sequencechemically synthesized 601atacgggagc
caacaccacc tgccttacac ctttacactt ccgataaatt gccgagagca 60ggtgtgacgg
at
7260272DNAArtificial Sequencechemically synthesized 602atccgtcaca
cctgctctcg gcaatttatc ggaagtgtaa aggtgtaagg caggtggtgt 60tggctcccgt
at
7260372DNAArtificial Sequencechemically synthesized 603atacgggagc
caacaccata aagtcaattc caacgcagac catcctcacc cccaagagca 60ggtgtgacgg
at
7260472DNAArtificial Sequencechemically synthesized 604atccgtcaca
cctgctcttg ggggtgagga tggtctgcgt tggaattgac tttatggtgt 60tggctcccgt
at
7260571DNAArtificial Sequencechemically synthesized 605atacgggagc
caacaccaaa ggacgccccc cttcgtcccg cgctcggtag caaagagcag 60gtgtgacgga t
7160671DNAArtificial Sequencechemically synthesized 606atccgtcaca
cctgctcttt gctaccgagc gcgggacgaa ggggggcgtc ctttggtgtt 60ggctcccgta t
7160772DNAArtificial Sequencechemically synthesized 607atacgggagc
caacaccatg tcccttgggc acacacaacc aatccccact ctccagagca 60ggtgtgacgg
at
7260872DNAArtificial Sequencechemically synthesized 608atccgtcaca
cctgctctgg agagtgggga ttggttgtgt gtgcccaagg gacatggtgt 60tggctcccgt
at
7260972DNAArtificial Sequencechemically synthesized 609atacgggagc
caacaccacc ctgctggctt cggtccgttt catggtctgc aagcagagca 60ggtgtgacgg
at
7261072DNAArtificial Sequencechemically synthesized 610atccgtcaca
cctgctctgc ttgcagacca tgaaacggac cgaagccagc agggtggtgt 60tggctcccgt
at
7261172DNAArtificial Sequencechemically synthesized 611atacgggagc
caacaccaca ctaccgtccc accccctccc agctcctccg gccgagagca 60ggtgtgacgg
ag
7261272DNAArtificial Sequencechemically synthesized 612atccgtcaca
cctgctctcg gccggaggag ctgggagggg gtgggacggt agtgtggtgt 60tggctcccgt
at
7261372DNAArtificial Sequencechemically synthesized 613atacgggagc
caacaccatg ctgcgggacc gccatctacc tgttcatgtg tctgagagca 60ggtgtgacgg
at
7261472DNAArtificial Sequencechemically synthesized 614atccgtcaca
cctgctctca gacacatgaa caggtagatg gcggtcccgc agcatggtgt 60tggctcccgt
at
7261571DNAArtificial Sequencechemically synthesized 615atacgggagc
caacaccagg atgcggctta cattacgcct gtttgtcatc tggagagcag 60gtgtgacgga t
7161671DNAArtificial Sequencechemically synthesized 616atccgtcaca
cctgctctcc agatgacaaa caggcgtaat gtaagccgca tcctggtgtt 60ggctcccgta t
7161772DNAArtificial Sequencechemically synthesized 617atacgggagc
caacaccagt gccctggaga cggactgtcg gggatcgttc actcagagca 60ggtgtgacgg
at
7261872DNAArtificial Sequencechemically synthesized 618atccgtcaca
cctgctctga gtgaacgatc cccgacagtc cgtctccagg gcactggtgt 60tggctcccgt
at
7261972DNAArtificial Sequencechemically synthesized 619atacgggagc
caacaccata ttctgcctgg agatctagtc ccctgctctc ccacagagca 60ggtgtgacgg
at
7262072DNAArtificial Sequencechemically synthesized 620atccgtcaca
cctgctctgt gggagagcag gggactagat ctccaggcag aatatggtgt 60tggctcccgt
at
7262172DNAArtificial Sequencechemically synthesized 621atacgggagc
caacaccatc aagggcttca ctcataaatt attaatgtgt ccccagagca 60ggtgtgacgg
at
7262272DNAArtificial Sequencechemically synthesized 622atccgtcaca
cctgctctgg ggacacatta ataatttatg agtgaagccc ttgatggtgt 60tggctcccgt
at
7262372DNAArtificial Sequencechemically synthesized 623atacgggagc
caacaccacc cactctcccc ccgctcccgc tcctccgctc cgcgagagca 60ggtgtgacgg
at
7262472DNAArtificial Sequencechemically synthesized 624atccgtcaca
cctgctctcg cggagcggag gagcgggagc ggggggagag tgggtggtgt 60tggctcccgt
at
7262572DNAArtificial Sequencechemically synthesized 625atacgggagc
caacaccata tctatgtact accacaatca agttcttcct cactagagca 60ggtgtgacgg
at
7262672DNAArtificial Sequencechemically synthesized 626atccgtcaca
cctgctctag tgaggaagaa cttgattgtg gtagtacata gatatggtgt 60tggctcccgt
at
7262772DNAArtificial Sequencechemically synthesized 627atacgggagc
caacaccacc ttttaaaacg ctagctagct tagtccattc caccagagca 60ggtgtgacgg
at
7262872DNAArtificial Sequencechemically synthesized 628atccgtcaca
cctgctctgg tggaatggac taagctagct agcgttttaa aaggtggtgt 60tggctcccgt
at
7262972DNAArtificial Sequencechemically synthesized 629atacgggagc
caacaccatt agtgatcggt ggctcaaact tacgctcgct ccgaagagca 60ggtgtgacgg
at
7263072DNAArtificial Sequencechemically synthesized 630atccgtcaca
cctgctcttc ggagcgagcg taagtttgag ccaccgatca ctaatggtgt 60tggctcccgt
at
7263172DNAArtificial Sequencechemically synthesized 631atacgggagc
caacaccagt cgtggttcgc cgtttactga cgttccagtt gccaagagca 60ggtgtgacgg
at
7263272DNAArtificial Sequencechemically synthesized 632atccgtcaca
cctgctcttg gcaactggaa cgtcagtaaa cggcgaacca cgactggtgt 60tggctcccgt
at
7263372DNAArtificial Sequencechemically synthesized 633atacgggagc
caacaccaat ggttttccta cgacaagaat agaaaaattg ccgaagagca 60ggtgtgacgg
at
7263472DNAArtificial Sequencechemically synthesized 634atccgtcaca
cctgctcttc ggcaattttt ctattcttgt cgtaggaaaa ccattggtgt 60tggctcccgt
at
7263572DNAArtificial Sequencechemically synthesized 635atacgggagc
caacaccact tcatcttctg tactttattt ggcagacccc tcccagagca 60ggtgtgacgg
at
7263672DNAArtificial Sequencechemically synthesized 636atccgtcaca
cctgctctgg gaggggtctg ccaaataaag tacagaagat gaagtggtgt 60tggctcccgt
at
7263771DNAArtificial Sequencechemically synthesized 637atacgggagc
caacaccacc ttttaaaacg ctagctagct tatccattcc accagagcag 60gtgtgacgga t
7163871DNAArtificial Sequencechemically synthesized 638atccgtcaca
cctgctctgg tggaatggat aagctagcta gcgttttaaa aggtggtgtt 60ggctcccgta t
7163972DNAArtificial Sequencechemically synthesized 639atacgggagc
caacaccaga tagtgttcca ccttatacgg agccccctca ctgaagagca 60ggtgtgacgg
at
7264072DNAArtificial Sequencechemically synthesized 640atccgtcaca
cctgctcttc agtgaggggg ctccgtataa ggtggaacac tatctggtgt 60tggctcccgt
at
7264172DNAArtificial Sequencechemically synthesized 641atacgggagc
caacaccaac gcacatcagg agaatgaaga actcaccccc ccccagagca 60ggtgtgacgg
at
7264272DNAArtificial Sequencechemically synthesized 642atccgtcaca
cctgctctgg gggggggtga gttcttcatt ctcctgatgt gcgttggtgt 60tggctcccgt
at
7264372DNAArtificial Sequencechemically synthesized 643atacgggagc
caacaccact aacatgcgcg agggctctgt ccccgtccga catcagagca 60ggtgtgacgg
at
7264472DNAArtificial Sequencechemically synthesized 644atccgtcaca
cctgctctga tgtcggacgg ggacagagcc ctcgcgcatg ttagtggtgt 60tggctcccgt
at
7264572DNAArtificial Sequencechemically synthesized 645atacgggagc
caacaccaac ctggctatct gcatgcggtc ggtcgccttg ttggagagca 60ggtgtgacgg
at
7264672DNAArtificial Sequencechemically synthesized 646atccgtcaca
cctgctctcc aacaaggcga ccgaccgcat gcagatagcc aggttggtgt 60tggctcccgt
at
7264772DNAArtificial Sequencechemically synthesized 647atacgggagc
caacaccacg taccctcaac ctttaacttc tcataaccgg ctgtagagca 60ggtgtgacgg
at
7264872DNAArtificial Sequencechemically synthesized 648atccgtcaca
cctgctctac agccggttat gagaagttaa aggttgaggg tacgtggtgt 60tggctcccgt
at
7264972DNAArtificial Sequencechemically synthesized 649atacgggagc
caacaccatg cgtgcctaat gccggccatt ctactgcttg gcctagagca 60ggtgtgacgg
at
7265072DNAArtificial Sequencechemically synthesized 650atccgtcaca
cctgctctag gccaagcagt agaatggccg gcattaggca cgcatggtgt 60tggctcccgt
at
7265172DNAArtificial Sequencechemically synthesized 651atacgggagc
caacaccatg tgtactaatg tagctgttgc aacgttccta acttagagca 60ggtgtgacgg
at
7265272DNAArtificial Sequencechemically synthesized 652atccgtcaca
cctgctctaa gttaggaacg ttgcaacagc tacattagta cacatggtgt 60tggctcccgt
at
7265372DNAArtificial Sequencechemically synthesized 653atacgggagc
caacaccact ggtgaggcgc ctgcgccgac tggccgtccc cccgagagca 60ggtgtgacgg
at
7265472DNAArtificial Sequencechemically synthesized 654atccgtcaca
cctgctctcg gggggacggc cagtcggcgc aggcgcctca ccagtggtgt 60tggctcccgt
at
7265572DNAArtificial Sequencechemically synthesized 655atacgggagc
caacaccagg gggcctcgtt ctacgagcct gggtgtgtcc ctccagagca 60ggtgtgacgg
at
7265672DNAArtificial Sequencechemically synthesized 656atccgtcaca
cctgctctgg agggacacac ccaggctcgt agaacgaggc cccctggtgt 60tggctcccgt
at
7265772DNAArtificial Sequencechemically synthesized 657atacgggagc
caacaccatt gctgggccca cctatcggcg taccgaaccc ccgcagagca 60ggtgtgacgg
at
7265872DNAArtificial Sequencechemically synthesized 658atccgtcaca
cctgctctgc gggggttcgg tacgccgata ggtgggccca gcaatggtgt 60tggctcccgt
at
7265972DNAArtificial Sequencechemically synthesized 659atacgggagc
caacaccacg ccgggacccc cccacgatgg ctgcctatat gtccagagca 60ggtgtgacgg
at
7266072DNAArtificial Sequencechemically synthesized 660atccgtcaca
cctgctctgg acatataggc agccatcgtg ggggggtccc ggcgtggtgt 60tggctcccgt
at
7266172DNAArtificial Sequencechemically synthesized 661atacgggagc
caacaccacc ctacttatca tgtccttatc ccgccttact ggccagagca 60ggtgtgacgg
at
7266272DNAArtificial Sequencechemically synthesized 662atccgtcaca
cctgctctgg ccagtaaggc gggataagga catgataagt agggtggtgt 60tggctcccgt
at
7266372DNAArtificial Sequencechemically synthesized 663atacgggagc
caacaccatt accactcctt acttgtacgt gaccttgtac caccagagca 60ggtgtgacgg
at
7266472DNAArtificial Sequencechemically synthesized 664atccgtcaca
cctgctctgg tggtacaagg tcacgtacaa gtaaggagtg gtaatggtgt 60tggctcccgt
at
7266572DNAArtificial Sequencechemically synthesized 665atacgggagc
caacaccatt cctgctaact tgctgcctgc cctccacggg gctcagagca 60ggtgtgacgg
at
7266672DNAArtificial Sequencechemically synthesized 666atccgtcaca
cctgctctga gccccgtgga gggcaggcag caagttagca ggaatggtgt 60tggctcccgt
at
7266772DNAArtificial Sequencechemically synthesized 667atacgggagc
caacaccatc tcatggtagc tgcgctggtt ttgtgcgtga tattagagca 60ggtgtgacgg
at
7266872DNAArtificial Sequencechemically synthesized 668atccgtcaca
cctgctctaa tatcacgcac aaaaccagcg cagctaccat gagatggtgt 60tggctcccgt
at
7266972DNAArtificial Sequencechemically synthesized 669atacgggagc
caacaccatg taaactttca ggttggatag tacgggctca cacaagagca 60ggtgtgacgg
at
7267072DNAArtificial Sequencechemically synthesized 670atccgtcaca
cctgctcttg tgtgagcccg tactatccaa cctgaaagtt tacatggtgt 60tggctcccgt
at
7267172DNAArtificial Sequencechemically synthesized 671atacgggagc
caacaccatg tgtccgccct tgtttttgtg tcgtccggca atcgagagca 60ggtgtgacgg
at
7267272DNAArtificial Sequencechemically synthesized 672atccgtcaca
cctgctctcg attgccggac gacacaaaaa caagggcgga cacatggtgt 60tggctcccgt
at
7267371DNAArtificial Sequencechemically synthesized 673atacgggagc
caacaccagc agcataacgt ccgtatccca atccatgacg atcagagcag 60gtgtgacgga t
7167471DNAArtificial Sequencechemically synthesized 674atccgtcaca
cctgctctga tcgtcatgga ttgggatacg gacgttatgc tgctggtgtt 60ggctcccgta t
7167572DNAArtificial Sequencechemically synthesized 675atacgggagc
caacaccatt taggataagt ggtcacgccg tctcgattta actgagagca 60ggtgtgacgg
at
7267672DNAArtificial Sequencechemically synthesized 676atccgtcaca
cctgctctca gttaaatcga gacggcgtga ccacttatcc taaatggtgt 60tggctcccgt
at
7267772DNAArtificial Sequencechemically synthesized 677atacgggagc
caacaccaat tccgtatcca tgctctggct gcgtatcccc tttgagagca 60ggtgtgacgg
at
7267872DNAArtificial Sequencechemically synthesized 678atccgtcaca
cctgctctca aaggggatac gcagccagag catggatacg gaattggtgt 60tggctcccgt
at
7267972DNAArtificial Sequencechemically synthesized 679atacgggagc
caacaccagg aaccggaata gggaatcccc cttaccaacc ccccagagca 60ggtgtgacgg
at
7268072DNAArtificial Sequencechemically synthesized 680atccgtcaca
cctgctctgg ggggttggta agggggattc cctattccgg ttcctggtgt 60tggctcccgt
at
7268172DNAArtificial Sequencechemically synthesized 681atacgggagc
caacaccaga acaccgcccc ctgtctgttc caattgctgt tatcagagca 60ggtgtgacgg
at
7268272DNAArtificial Sequencechemically synthesized 682atccgtcaca
cctgctctga taacagcaat tggaacagac agggggcggt gttctggtgt 60tggctcccgt
at
7268372DNAArtificial Sequencechemically synthesized 683atacgggagc
caacaccacc cggttttaac ttttgttggt atgtctcgaa tcccagagca 60ggtgtgacgg
at
7268472DNAArtificial Sequencechemically synthesized 684atccgtcaca
cctgctctgg gattcgagac ataccaacaa aagttaaaac cgggtggtgt 60tggctcccgt
at
7268571DNAArtificial Sequencechemically synthesized 685atacgggagc
caacaccaaa tagagtattc gaaagtagcg tacttcaggt cctagagcag 60gtgtgacgga t
7168671DNAArtificial Sequencechemically synthesized 686atccgtcaca
cctgctctag gacctgaagt acgctacttt cgaatactct atttggtgtt 60ggctcccgta t
7168770DNAArtificial Sequencechemically synthesized 687atacgggagc
caacaccacc gaattgattg ataaaaaggg ttagatcgct ccagagcagg 60tgtgacggat
7068870DNAArtificial Sequencechemically synthesized 688atccgtcaca
cctgctctgg agcgatctaa ccctttttat caatcaattc ggtggtgttg 60gctcccgtat
7068971DNAArtificial Sequencechemically synthesized 689atacgggagc
caacaccatt ggcgacagag ttcgacgtcc cctagtggca gagagagcag 60gtgtgacgga t
7169071DNAArtificial Sequencechemically synthesized 690atccgtcaca
cctgctctct ctgccactag gggacgtcga actctgtcgc caatggtgtt 60ggctcccgta t
7169172DNAArtificial Sequencechemically synthesized 691atacgggagc
caacaccaat cgctctcaca aatttgccct agactcatac catcagagca 60ggtgtgacgg
at
7269272DNAArtificial Sequencechemically synthesized 692atccgtcaca
cctgctctga tggtatgagt ctagggcaaa tttgtgagag cgattggtgt 60tggctcccgt
at
7269371DNAArtificial Sequencechemically synthesized 693atacgggagc
ccacaccaca actttgaaga tccttgagcc gcgctcacca cctagagcag 60gtgtgacgga t
7169471DNAArtificial Sequencechemically synthesized 694atccgtcaca
cctgctctag gtggtgagcg cggctcaagg atcttcaaag ttgtggtgtg 60ggctcccgta t
7169572DNAArtificial Sequencechemically synthesized 695atacgggagc
caacaccaca aagtttagcg ttatgcaact cccccttata ctcgagagca 60ggtgtgacgg
at
7269672DNAArtificial Sequencechemically synthesized 696atccgtcaca
cctgctctcg agtataaggg ggagttgcat aacgctaaac tttgtggtgt 60tggctcccgt
at
7269772DNAArtificial Sequencechemically synthesized 697atacgggagc
caacaccaca aagtttagcg ttatgcaact cccccttata ctcgagagca 60ggtgtgacgg
at
7269872DNAArtificial Sequencechemically synthesized 698atccgtcaca
cctgctctcg agtataaggg ggagttgcat aacgctaaac tttgtggtgt 60tggctcccgt
at
7269971DNAArtificial Sequencechemically synthesized 699atacgggagc
caacaccata cggctatacc cgcccgacgt acaccacatc cccagagcag 60gtgtgacgga t
7170071DNAArtificial Sequencechemically synthesized 700atccgtcaca
cctgctctgg ggatgtggtg tacgtcgggc gggtatagcc gtatggtgtt 60ggctcccgta t
7170172DNAArtificial Sequencechemically synthesized 701atacgggagc
caacaccagt gtaacttgga gccctgacga gctcttcccg tagcagagca 60ggtgtgacgg
at
7270272DNAArtificial Sequencechemically synthesized 702atccgtcaca
cctgctctgc tacgggaaga gctcgtcagg gctccaagtt acactggtgt 60tggctcccgt
at
7270372DNAArtificial Sequencechemically synthesized 703atacgggagc
caacaccata gtttagcatg ctcaatagta caccagatca gtggagagca 60ggtgtgacgg
at
7270472DNAArtificial Sequencechemically synthesized 704atccgtcaca
cctgctctcc actgatctgg tgtactattg agcatgctaa actatggtgt 60tggctcccgt
at
7270572DNAArtificial Sequencechemically synthesized 705atacgggagc
caacaccatt cgtgtgtctg tctaagtgga actgcctgta attaagagca 60ggtgtgacgg
at
7270672DNAArtificial Sequencechemically synthesized 706atccgtcaca
cctgctctta attacaggca gttccactta gacagacaca cgaatggtgt 60tggctcccgt
at
7270772DNAArtificial Sequencechemically synthesized 707atacgggagc
caacaccaca tgagtggcgg gcttagcggg ctgcaaggcg cgttagagca 60ggtgtgacgg
at
7270872DNAArtificial Sequencechemically synthesized 708atccgtcaca
cctgctctaa cgcgccttgc agcccgctaa gcccgccact catgtggtgt 60tggctcccgt
at
7270972DNAArtificial Sequencechemically synthesized 709atacgggagc
caacaccatg tggcataaac agacgaattt gtcctataac gagtagagca 60ggtgtgacgg
at
7271072DNAArtificial Sequencechemically synthesized 710atccgtcaca
cctgctctac tcgttatagg acaaattcgt ctgtttatgc cacatggtgt 60tggctcccgt
at
7271172DNAArtificial Sequencechemically synthesized 711atacgggagc
caacaccact tattaccgat ttagcttgag actcctcccc tacaagagca 60ggtgtgacgg
at
7271272DNAArtificial Sequencechemically synthesized 712atccgtcaca
cctgctcttg taggggagga gtctcaagct aaatcggtaa taagtggtgt 60tggctcccgt
at
7271372DNAArtificial Sequencechemically synthesized 713atacgggagc
caacaccatc gttttgtact ttatgagccg cacggagccc ccccagagca 60ggtgtgacgg
at
7271472DNAArtificial Sequencechemically synthesized 714atccgtcaca
cctgctctgg gggggctccg tgcggctcat aaagtacaaa acgatggtgt 60tggctcccgt
at
7271572DNAArtificial Sequencechemically synthesized 715atacgggagc
caacaccatc tcgctaagct ctgttgggac taacttccgc tattagagca 60ggtgtgacgg
at
7271672DNAArtificial Sequencechemically synthesized 716atccgtcaca
cctgctctaa tagcggaagt tagtcccaac agagcttagc gagatggtgt 60tggctcccgt
at
7271772DNAArtificial Sequencechemically synthesized 717atacgggagc
caacaccatc caccctacgt cataatatga aatgaaacat atatagagca 60ggtgtgacgg
at
7271872DNAArtificial Sequencechemically synthesized 718atccgtcaca
cctgctctat atatgtttca tttcatatta tgacgtaggg tggatggtgt 60tggctcccgt
at
7271972DNAArtificial Sequencechemically synthesized 719atacgggagc
caacaccacc cactctcccc ccgctcccgc tcccccgctc cgcgagagca 60ggtgtgacgg
at
7272072DNAArtificial Sequencechemically synthesized 720atccgtcaca
cctgctctcg cggagcgggg gagcgggagc ggggggagag tgggtggtgt 60tggctcccgt
at
7272172DNAArtificial Sequencechemically synthesized 721atacgggagc
caacaccaat agttgcgtct tgtcatgtac gcctccttgc cagcagagca 60ggtgtgacgg
at
7272272DNAArtificial Sequencechemically synthesized 722atccgtcaca
cctgctctgc tggcaaggag gcgtacatga caagacgcaa ctattggtgt 60tggctcccgt
at
7272372DNAArtificial Sequencechemically synthesized 723atacgggagc
caacaccagc gggtttctta gttctgattg gacgctctgt ttgcagagca 60ggtgtgacgg
at
7272472DNAArtificial Sequencechemically synthesized 724atccgtcaca
cctgctctgc aaacagagcg tccaatcaga actaagaaac ccgctggtgt 60tggctcccgt
at
7272572DNAArtificial Sequencechemically synthesized 725atacgggagc
caacaccaag agcacctgca ccctcccgcc ctctattgca ttctagagca 60ggtgtgacgg
at
7272672DNAArtificial Sequencechemically synthesized 726atccgtcaca
cctgctctag aatgcaatag agggcgggag ggtgcaggtg ctcttggtgt 60tggctcccgt
at
7272772DNAArtificial Sequencechemically synthesized 727atacgggagc
caacaccaca tcataccttg ttctaccggc agcctctcta aataagagca 60ggtgtgacgg
at
7272872DNAArtificial Sequencechemically synthesized 728atccgtcaca
cctgctctta tttagagagg ctgccggtag aacaaggtat gatgtggtgt 60tggctcccgt
at
7272972DNAArtificial Sequencechemically synthesized 729atacgggagc
caacaccatt ccaaatgaac agttctcgat cccacttctc atctagagca 60ggtgtgacgg
at
7273072DNAArtificial Sequencechemically synthesized 730atccgtcaca
cctgctctag atgagaagtg ggatcgagaa ctgttcattt ggaatggtgt 60tggctcccgt
at
7273172DNAArtificial Sequencechemically synthesized 731atacgggagc
caacaccaga cctactgccc ccctgtctcc ctttgtctgc ccttagagca 60ggtgtgacgg
at
7273272DNAArtificial Sequencechemically synthesized 732atccgtcaca
cctgctctaa gggcagacaa agggagacag gggggcagta ggtctggtgt 60tggctcccgt
at
7273372DNAArtificial Sequencechemically synthesized 733atacgggagc
caacaccaac catcccggaa tgtgggtttt atgtaaatat aagcagagca 60ggtgtgacgg
at
7273472DNAArtificial Sequencechemically synthesized 734atccgtcaca
cctgctctgc ttatatttac ataaaaccca cattccggga tggttggtgt 60tggctcccgt
at
7273572DNAArtificial Sequencechemically synthesized 735atacgggagc
caacaccacc cctaactcct atatttaatc cttatcaaat ttctagagca 60ggtgtgacgg
at
7273672DNAArtificial Sequencechemically synthesized 736atccgtcaca
cctgctctag aaatttgata aggattaaat ataggagtta ggggtggtgt 60tggctcccgt
at
7273772DNAArtificial Sequencechemically synthesized 737atacgggagc
caacaccacc agcagtaaag ttccagcatt gcgcttaata gactagagca 60ggtgtgacgg
at
7273872DNAArtificial Sequencechemically synthesized 738atccgtcaca
cctgctctag tctattaagc gcaatgctgg aactttactg ctggtggtgt 60tggctcccgt
at
7273972DNAArtificial Sequencechemically synthesized 739atacgggagc
caacaccaag tccaagccaa acaagagcat aacaccaaat ctggagagca 60ggtgtgacgg
at
7274072DNAArtificial Sequencechemically synthesized 740atccgtcaca
cctgctctcc agatttggtg ttatgctctt gtttggcttg gacttggtgt 60tggctcccgt
at
7274172DNAArtificial Sequencechemically synthesized 741atacgggagc
caacaccact caatacgtct tatcgtctct gagtacatct ggccagagca 60ggtgtgacgg
at
7274272DNAArtificial Sequencechemically synthesized 742atccgtcaca
cctgctctgg ccagatgtac tcagagacga taagacgtat tgagtggtgt 60tggctcccgt
at
7274372DNAArtificial Sequencechemically synthesized 743atacgggagc
caacaccagg gccgctgcga tttctacgct acctgccgtt ggtaagagca 60ggtgtgacgg
at
7274472DNAArtificial Sequencechemically synthesized 744atccgtcaca
cctgctctta ccaacggcag gtagcgtaga aatcgcagcg gccctggtgt 60tggctcccgt
at
7274572DNAArtificial Sequencechemically synthesized 745atacgggagc
caacaccaca accggctaat gacaaatcaa agattttttc ggcgagagca 60ggtgtgacgg
at
7274672DNAArtificial Sequencechemically synthesized 746atccgtcaca
cctgctctcg ccgaaaaaat ctttgatttg tcattagccg gttgtggtgt 60tggctcccgt
at
7274772DNAArtificial Sequencechemically synthesized 747atacgggagc
caacaccagc acaacttaag tgcaagcaaa ttcggattaa ccaaagagca 60ggtgtgacgg
at
7274872DNAArtificial Sequencechemically synthesized 748atccgtcaca
cctgctcttt ggttaatccg aatttgcttg cacttaagtt gtgctggtgt 60tggctcccgt
at
7274972DNAArtificial Sequencechemically synthesized 749atacgggagc
caacaccatc cgaacagtta gtttaagacc gtcctccttt cttaagagca 60ggtgtgacgg
at
7275072DNAArtificial Sequencechemically synthesized 750atccgtcaca
cctgctctta agaaaggagg acggtcttaa actaactgtt cggatggtgt 60tggctcccgt
at
7275172DNAArtificial Sequencechemically synthesized 751atacgggagc
caacaccact tcaaaagtca gatacaaaga cagagattgg acttagagca 60ggtgtgacgg
at
7275272DNAArtificial Sequencechemically synthesized 752atccgtcaca
cctgctctaa gtccaatctc tgtctttgta tctgactttt gaagtggtgt 60tggctcccgt
at
7275371DNAArtificial Sequencechemically synthesized 753atacgggagc
caacaccact tccgttataa cgtttctgga atttactacg attagagcag 60gtgtgacgga t
7175471DNAArtificial Sequencechemically synthesized 754atccgtcaca
cctgctctaa tcgtagtaaa ttccagaaac gttataacgg aagtggtgtt 60ggctcccgta t
7175571DNAArtificial Sequencechemically synthesized 755atacgggagc
caacaccaac cacggatgtt gaatatccga cgatcacgta attagagcag 60gtgtgacgga t
7175671DNAArtificial Sequencechemically synthesized 756atccgtcaca
cctgctctaa ttacgtgatc gtcggatatt caacatccgt ggttggtgtt 60ggctcccgta t
7175771DNAArtificial Sequencechemically synthesized 757atacgggagc
caacaccata gacttaagct ctctccagga gcttgtcgct tgcagagcag 60gtgtgacgga t
7175871DNAArtificial Sequencechemically synthesized 758atccgtcaca
cctgctctgc aagcgacaag ctcctggaga gagcttaagt ctatggtgtt 60ggctcccgta t
7175972DNAArtificial Sequencechemically synthesized 759atacgggagc
caacaccacg ggggcatctt ccattaaccc attacctcac cccaagagca 60ggtgtgacgg
at
7276072DNAArtificial Sequencechemically synthesized 760atccgtcaca
cctgctcttg gggtgaggta atgggttaat ggaagatgcc cccgtggtgt 60tggctcccgt
at
7276171DNAArtificial Sequencechemically synthesized 761atacgggagc
caacaccaca cagacaacca ttgattacgg ggacaattcg ggcagagcag 60gtgtgacgga t
7176271DNAArtificial Sequencechemically synthesized 762atccgtcaca
cctgctctgc ccgaattgtc cccgtaatca atggttgtct gtgtggtgtt 60ggctcccgta t
7176371DNAArtificial Sequencechemically synthesized 763atacgggagc
caacaccact ggtaggtgag tgtgtgtctg tactcgcatc gttagagcag 60gtgtgacgga t
7176471DNAArtificial Sequencechemically synthesized 764atccgtcaca
cctgctctaa cgatgcgagt acagacacac actcacctac cagtggtgtt 60ggctcccgta t
7176570DNAArtificial Sequencechemically synthesized 765atacgggagc
caacaccacc gaattgattg ataaaaaggg ttagatcgct ccagagcagg 60tgtgacggat
7076670DNAArtificial Sequencechemically synthesized 766atccgtcaca
cctgctctgg agcgatctaa ccctttttat caatcaattc ggtggtgttg 60gctcccgtat
7076771DNAArtificial Sequencechemically synthesized 767atacgggagc
caacaccacc taggccccca acatgtgtgg tataagtttc cgtagagcag 60gtgtgacgga t
7176871DNAArtificial Sequencechemically synthesized 768atccgtcaca
cctgctctac ggaaacttat accacacatg ttgggggcct aggtggtgtt 60ggctcccgta t
7176971DNAArtificial Sequencechemically synthesized 769atacgggagc
caacaccatc agaaactata gtgccatagt tttatctttg tacagagcag 60gtgtgacgga t
7177071DNAArtificial Sequencechemically synthesized 770atccgtcaca
cctgctctgt acaaagataa aactatggca ctatagtttc tgatggtgtt 60ggctcccgta t
7177172DNAArtificial Sequencechemically synthesized 771atacgggagc
caacaccaat agatggataa gggggaaact gccattcggt tagtagagca 60ggtgtgacgg
at
7277272DNAArtificial Sequencechemically synthesized 772atccgtcaca
cctgctctac taaccgaatg gcagtttccc ccttatccat ctattggtgt 60tggctcccgt
at
7277372DNAArtificial Sequencechemically synthesized 773atacgggagc
caacaccacc aacgaatact accaggccta gcacaataca caacagagca 60ggtgtgacgg
at
7277472DNAArtificial Sequencechemically synthesized 774atccgtcaca
cctgctctgt tgtgtattgt gctaggcctg gtagtattcg ttggtggtgt 60tggctcccgt
at
7277571DNAArtificial Sequencechemically synthesized 775atacgggagc
caacaccaaa cttgagatat gggcgctatg ttccttttca tccagagcag 60gtgtgacgga t
7177671DNAArtificial Sequencechemically synthesized 776atccgtcaca
cctgctctgg atgaaaagga acatagcgcc catatctcaa gtttggtgtt 60ggctcccgta t
7177771DNAArtificial Sequencechemically synthesized 777atacgggagc
caacaccagc cgaccggtaa gtttatataa acttccgccg cctagagcag 60gtgtgacgga t
7177871DNAArtificial Sequencechemically synthesized 778atccgtcaca
cctgctctag gcggcggaag tttatataaa cttaccggtc ggctggtgtt 60ggctcccgta t
7177971DNAArtificial Sequencechemically synthesized 779atacgggagc
caacaccatg actcacgctt ctcgcccgca cgaagagtct ccaagagcag 60gtgtgacgga t
7178071DNAArtificial Sequencechemically synthesized 780atccgtcaca
cctgctcttg gagactcttc gtgcgggcga gaagcgtgag tcatggtgtt 60ggctcccgta t
7178171DNAArtificial Sequencechemically synthesized 781atacgggagc
caacaccatg acgaccatgc aagaaatccg acaattcctc acgagagcag 60gtgtgacgga t
7178271DNAArtificial Sequencechemically synthesized 782atccgtcaca
cctgctctcg tgaggaattg tcggatttct tgcatggtcg tcatggtgtt 60ggctcccgta t
7178371DNAArtificial Sequencechemically synthesized 783atacgggagc
caacaccaag catttagatt cgctccttga acccacttcc ttcagagcag 60gtgtgacgga t
7178471DNAArtificial Sequencechemically synthesized 784atccgtcaca
cctgctctga aggaagtggg ttcaaggagc gaatctaaat gcttggtgtt 60ggctcccgta t
7178571DNAArtificial Sequencechemically synthesized 785atacgggagc
caacaccagg tgcttctctt acgtggccgc tattccaaag tgaagagcag 60gtgtgacgga t
7178671DNAArtificial Sequencechemically synthesized 786atccgtcaca
cctgctcttc actttggaat agcggccacg taagagaagc acctggtgtt 60ggctcccgta t
7178771DNAArtificial Sequencechemically synthesized 787atacgggagc
caacaccagc ccgggcatgt cgtttgtatg aagctagcta actagagcag 60gtgtgacgga t
7178871DNAArtificial Sequencechemically synthesized 788atccgtcaca
cctgctctag ttagctagct tcatacaaac gacatgcccg ggctggtgtt 60ggctcccgta t
7178971DNAArtificial Sequencechemically synthesized 789atacgggagc
caacaccatg accatcaata cagacaaata gaatcgcgtt atgagagcag 60gtgtgacgga t
7179071DNAArtificial Sequencechemically synthesized 790atccgtcaca
cctgctctca taacgcgatt ctatttgtct gtattgatgg tcatggtgtt 60ggctcccgta t
7179172DNAArtificial Sequencechemically synthesized 791atacgggagc
caacaccatg agggtttcct cacatgggga ggtcctccat gtggagagca 60ggtgtgacgg
at
7279272DNAArtificial Sequencechemically synthesized 792atccgtcaca
cctgctctcc acatggagga cctccccatg tgaggaaacc ctcatggtgt 60tggctcccgt
at
7279371DNAArtificial Sequencechemically synthesized 793atacgggagc
caacaccagg actaacatta taagaattgc gaataatcat tggagagcag 60gtgtgacgga t
7179471DNAArtificial Sequencechemically synthesized 794atccgtcaca
cctgctctcc aatgattatt cgcaattctt ataatgttag tcctggtgtt 60ggctcccgta t
7179571DNAArtificial Sequencechemically synthesized 795atacgggagc
caacaccatt gtaccttaac tatactcagc tacattatgc caaagagcag 60gtgtgacgga t
7179671DNAArtificial Sequencechemically synthesized 796atccgtcaca
cctgctcttt ggcataatgt agctgagtat agttaaggta caatggtgtt 60ggctcccgta t
7179771DNAArtificial Sequencechemically synthesized 797atacgggagc
caacaccact catgttggat attacgaaca gtgcgttagg ctgagagcag 60gtgtgacgga t
7179871DNAArtificial Sequencechemically synthesized 798atccgtcaca
cctgctctca gcctaacgca ctgttcgtaa tatccaacat gagtggtgtt 60ggctcccgta t
7179971DNAArtificial Sequencechemically synthesized 799atacgggagc
caacaccatg aacgcatcac agttgcttca cactcgacta gctagagcag 60gtgtgacgga t
7180071DNAArtificial Sequencechemically synthesized 800atccgtcaca
cctgctctag ctagtcgagt gtgaagcaac tgtgatgcgt tcatggtgtt 60ggctcccgta t
7180171DNAArtificial Sequencechemically synthesized 801atacgggagc
caacaccaaa aactactctc tacggatcgt acatcatggg cgtagagcag 60gtgtgacgga t
7180271DNAArtificial Sequencechemically synthesized 802atccgtcaca
cctgctctac gcccatgatg tacgatccgt agagagtagt ttttggtgtt 60ggctcccgta t
7180372DNAArtificial Sequencechemically synthesized 803atacgggagc
caacaccacc cactctcccc ccgctcccgc tcccccgctc cgcgagagca 60ggtgtgacgg
at
7280472DNAArtificial Sequencechemically synthesized 804atccgtcaca
cctgctctcg cggagcgggg gagcgggagc ggggggagag tgggtggtgt 60tggctcccgt
at
7280571DNAArtificial Sequencechemically synthesized 805atacgggagc
caacaccata gggcaattaa ggccaagagt gttggccgtc gctagagcag 60gtgtgacgga t
7180671DNAArtificial Sequencechemically synthesized 806atccgtcaca
cctgctctag cgacggccaa cactcttggc cttaattgcc ctatggtgtt 60ggctcccgta t
7180771DNAArtificial Sequencechemically synthesized 807atacgggagc
caacaccaaa acgggaaact gcatttccca cgcctcgtac cccagagcag 60gtgtgacgga t
7180871DNAArtificial Sequencechemically synthesized 808atccgtcaca
cctgctctgg ggtacgaggc gtgggaaatg cagtttcccg ttttggtgtt 60ggctcccgta t
7180971DNAArtificial Sequencechemically synthesized 809atacgggagc
caacaccaaa ccgaaggatc gccgggtcag acctatctca agcagagcag 60gtgtgacgga t
7181071DNAArtificial Sequencechemically synthesized 810atccgtcaca
cctgctctgc ttgagatagg tctgacccgg cgatccttcg gtttggtgtt 60ggctcccgta t
7181171DNAArtificial Sequencechemically synthesized 811atacgggagc
caacaccatt tgctgacgaa ccattggcta gcttgattgt cacagagcag 60gtgtgacgga t
7181271DNAArtificial Sequencechemically synthesized 812atccgtcaca
cctgctctgt gacaatcaag ctagccaatg gttcgtcagc aaatggtgtt 60ggctcccgta t
7181371DNAArtificial Sequencechemically synthesized 813atacgggagc
caacaccaga ctttttgctg ccatcccggg tgtcgttgca agtagagcag 60gtgtgacgga t
7181471DNAArtificial Sequencechemically synthesized 814atccgtcaca
cctgctctac ttgcaacgac acccgggatg gcagcaaaaa gtctggtgtt 60ggctcccgta t
7181572DNAArtificial Sequencechemically synthesized 815atacgggagc
caacaccacg catcccccgc cgggcccgcg ccccgctcgc agacagagca 60ggtgtgacgg
at
7281672DNAArtificial Sequencechemically synthesized 816atccgtcaca
cctgctctgt ctgcgagcgg ggcgcgggcc cggcggggga tgcgtggtgt 60tggctcccgt
at
7281771DNAArtificial Sequencechemically synthesized 817atacgggagc
caacaccaca atcattaaac ctttttactc ttcctgaatc accagagcag 60gtgtgacgga t
7181871DNAArtificial Sequencechemically synthesized 818atccgtcaca
cctgctctgg tgattcagga agagtaaaaa ggtttaatga ttgtggtgtt 60ggctcccgta t
7181971DNAArtificial Sequencechemically synthesized 819atacgggagc
caacaccaac ttatgcgacc cttccggtag ctggaggtta ttaagagcag 60gtgtgacgga t
7182071DNAArtificial Sequencechemically synthesized 820atccgtcaca
cctgctctta ataacctcca gctaccggaa gggtcgcata agttggtgtt 60ggctcccgta t
7182172DNAArtificial Sequencechemically synthesized 821atacgggagc
caacaccagg gccgcctctt tccgattcgt gggagccttc ttgaagagca 60ggtgtgacgg
at
7282272DNAArtificial Sequencechemically synthesized 822atccgtcaca
cctgctcttc aagaaggctc ccacgaatcg gaaagaggcg gccctggtgt 60tggctcccgt
at
7282372DNAArtificial Sequencechemically synthesized 823atacgggagc
caacaccatg ttccgtcaca ggtctcctgt tcttaatttg ttttagagca 60ggtgtgacgg
at
7282472DNAArtificial Sequencechemically synthesized 824atccgtcaca
cctgctctaa aacaaattaa gaacaggaga cctgtgacgg aacatggtgt 60tggctcccgt
at
7282571DNAArtificial Sequencechemically synthesized 825atacgggagc
caacaccata cgacctggaa actgctcgtc agagtctgaa atgagagcag 60gtgtgacgga t
7182671DNAArtificial Sequencechemically synthesized 826atccgtcaca
cctgctctca tttcagactc tgacgagcag tttccaggtc gtatggtgtt 60ggctcccgta t
7182771DNAArtificial Sequencechemically synthesized 827atacgggagc
caacaccaag ttgtcactgg atgacattcg ggttcgcttg cacagagcag 60gtgtgacgga t
7182871DNAArtificial Sequencechemically synthesized 828atccgtcaca
cctgctctgt gcaagcgaac ccgaatgtca tccagtgaca acttggtgtt 60ggctcccgta t
7182971DNAArtificial Sequencechemically synthesized 829atacgggagc
caacaccaaa gtggattgcg acgtgcttct accagatacc cggagagcag 60gtgtgacgga t
7183071DNAArtificial Sequencechemically synthesized 830atccgtcaca
cctgctctcc gggtatctgg tagaagcacg tcgcaatcca ctttggtgtt 60ggctcccgta t
7183171DNAArtificial Sequencechemically synthesized 831atacgggagc
caacaccagc acacggcacg cccctccgaa ccacgccccc gaaagagcag 60gtgtgacgga t
7183271DNAArtificial Sequencechemically synthesized 832atccgtcaca
cctgctcttt cgggggcgtg gttcggaggg gcgtgccgtg tgctggtgtt 60ggctcccgta t
7183371DNAArtificial Sequencechemically synthesized 833atacgggagc
caacaccagc gacgacagtt atataatccg agaatagtac atgagagcag 60gtgtgacgga t
7183471DNAArtificial Sequencechemically synthesized 834atccgtcaca
cctgctctca tgtactattc tcggattata taactgtcgt cgctggtgtt 60ggctcccgta t
7183570DNAArtificial Sequencechemically synthesized 835atacgggagc
caacaccagc acacggcacg ccccccgaac cacgcccccg aaagagcagg 60tgtgacggat
7083670DNAArtificial Sequencechemically synthesized 836atccgtcaca
cctgctcttt cgggggcgtg gttcgggggg cgtgccgtgt gctggtgttg 60gctcccgtat
7083771DNAArtificial Sequencechemically synthesized 837atacgggagc
caacaccata tttcaatctg ttgaaataat tcatacatgc ttaagagcag 60gtgtgacgga t
7183871DNAArtificial Sequencechemically synthesized 838atccgtcaca
cctgctctta agcatgtatg aattatttca acagattgaa atatggtgtt 60ggctcccgta t
7183972DNAArtificial Sequencechemically synthesized 839atacgggagc
caacaccatc ctcctgcgac gtctggagaa cagcctctac tttaagagca 60ggtgtgacgg
at
7284072DNAArtificial Sequencechemically synthesized 840atccgtcaca
cctgctctta aagtagaggc tgttctccag acgtcgcagg aggatggtgt 60tggctcccgt
at
7284171DNAArtificial Sequencechemically synthesized 841atacgggagc
caacaccagg ctgcgtacat gaacaggcac tacaaatgtc ccaagagcag 60gtgtgacgga t
7184271DNAArtificial Sequencechemically synthesized 842atccgtcaca
cctgctcttg ggacatttgt agtgcctgtt catgtacgca gcctggtgtt 60ggctcccgta t
7184372DNAArtificial Sequencechemically synthesized 843atacgggagc
caacaccaca gagagaagta cgtaacatca aaaacgcggt cagaagagca 60ggtgtgacgg
at
7284472DNAArtificial Sequencechemically synthesized 844atccgtcaca
cctgctcttc tgaccgcgtt tttgatgtta cgtacttctc tctgtggtgt 60tggctcccgt
at
7284570DNAArtificial Sequencechemically synthesized 845atacgggagc
caacaccaac acaaccgagc caatagacaa tcagtgtttc acagagcagg 60tgtgacggat
7084670DNAArtificial Sequencechemically synthesized 846atccgtcaca
cctgctctgt gaaacactga ttgtctattg gctcggttgt gttggtgttg 60gctcccgtat
7084772DNAArtificial Sequencechemically synthesized 847atacgggagc
caacaccacc ctaaattcca gagtgtacaa gagaacgaac taccagagca 60ggtgtgacgg
at
7284872DNAArtificial Sequencechemically synthesized 848atccgtcaca
cctgctctgg tagttcgttc tcttgtacac tctggaattt agggtggtgt 60tggctcccgt
at
7284972DNAArtificial Sequencechemically synthesized 849atacgggagc
caacaccaat agatggataa gggggaaact gccattcggt tagtagagca 60ggtgtgacgg
at
7285072DNAArtificial Sequencechemically synthesized 850atccgtcaca
cctgctctac taaccgaatg gcagtttccc ccttatccat ctattggtgt 60tggctcccgt
at
7285172DNAArtificial Sequencechemically synthesized 851atacgggagc
caacaccaaa acccgcacta catctcctct gcccccttct gataagagca 60ggtgtgacgg
at
7285272DNAArtificial Sequencechemically synthesized 852atccgtcaca
cctgctctta tcagaagggg gcagaggaga tgtagtgcgg gttttggtgt 60tggctcccgt
at
7285369DNAArtificial Sequencechemically synthesized 853atacgggagc
caacaccatc gaatggtctc ccaactaaag aaactctcca tccagagcag 60gtgtgacgg
6985469DNAArtificial Sequencechemically synthesized 854ccgtcacacc
tgctctggat ggagagtttc tttagttggg agaccattcg atggtgttgg 60ctcccgtat
6985572DNAArtificial Sequencechemically synthesized 855atacgggagc
caacaccacg ggggcatctt ccattaaccc attacctcac cccaagagca 60ggtgtgacgg
at
7285672DNAArtificial Sequencechemically synthesized 856atccgtcaca
cctgctcttg gggtgaggta atgggttaat ggaagatgcc cccgtggtgt 60tggctcccgt
at
7285771DNAArtificial Sequencechemically synthesized 857atacgggagc
caacaccatg gacgtactga ctgccccgta ctcttttcag ttgagagcag 60gtgtgacgga t
7185871DNAArtificial Sequencechemically synthesized 858atccgtcaca
cctgctctca actgaaaaga gtacggggca gtcagtacgt ccatggtgtt 60ggctcccgta t
7185971DNAArtificial Sequencechemically synthesized 859atacgggagc
caacaccaac taactactca agatgaacac tgtcggcgtt tacagagcag 60gtgtgacgga t
7186071DNAArtificial Sequencechemically synthesized 860atccgtcaca
cctgctctgt aaacgccgac agtgttcatc ttgagtagtt agttggtgtt 60ggctcccgta t
7186171DNAArtificial Sequencechemically synthesized 861atacgggagc
caacaccagc ccttaagcgt tgcctaacgg actctgaggc aatagagcag 60gtgtgacgga t
7186271DNAArtificial Sequencechemically synthesized 862atccgtcaca
cctgctctat tgcctcagag tccgttaggc aacgcttaag ggctggtgtt 60ggctcccgta t
7186371DNAArtificial Sequencechemically synthesized 863atacgggagc
caacaccaac ctcacttaca actcgatttt ttatggacag ccgagagcag 60gtgtgacgga t
7186471DNAArtificial Sequencechemically synthesized 864atccgtcaca
cctgctctcg gctgtccata aaaaatcgag ttgtaagtga ggttggtgtt 60ggctcccgta t
7186572DNAArtificial Sequencechemically synthesized 865atacgggagc
caacaccatt tgagcggcag ctaaccggcc gaccaattcg ctacagagca 60ggtgtgacgg
at
7286672DNAArtificial Sequencechemically synthesized 866atccgtcaca
cctgctctgt agcgaattgg tcggccggtt agctgccgct caaatggtgt 60tggctcccgt
at
7286772DNAArtificial Sequencechemically synthesized 867atacgggagc
caacaccacc ttttaaaacg ctagctagct tagtccattc caccagagca 60ggtgtgacgg
at
7286872DNAArtificial Sequencechemically synthesized 868atccgtcaca
cctgctctgg tggaatggac taagctagct agcgttttaa aaggtggtgt 60tggctcccgt
at
7286972DNAArtificial Sequencechemically synthesized 869atacgggagc
caacaccagc cctgggccag cccgtgactt tcccccggcg tccaagagca 60ggtgtgacgg
at
7287072DNAArtificial Sequencechemically synthesized 870atccgtcaca
cctgctcttg gacgccgggg gaaagtcacg ggctggccca gggctggtgt 60tggctcccgt
at
7287172DNAArtificial Sequencechemically synthesized 871atacgggagc
caacaccatc ctcctgcggc gtctggagaa cagcctctac tttaagagca 60ggtgtgacgg
at
7287272DNAArtificial Sequencechemically synthesized 872atccgtcaca
cctgctctta aagtagaggc tgttctccag acgccgcagg aggatggtgt 60tggctcccgt
at
7287372DNAArtificial Sequencechemically synthesized 873atacgggagc
caacaccacc aacgaatact accaggccta gcacaataca caacagagca 60ggtgtgacgg
at
7287472DNAArtificial Sequencechemically synthesized 874atccgtcaca
cctgctctgt tgtgtattgt gctaggcctg gtagtattcg ttggtggtgt 60tggctcccgt
at
7287572DNAArtificial Sequencechemically synthesized 875atacgggagc
caacaccacc gaatgtgctg caagactaat ctggatggcc atgcagagca 60ggtgtgacgg
at
7287672DNAArtificial Sequencechemically synthesized 876atccgtcaca
cctgctctgc atggccatcc agattagtct tgcagcacat tcggtggtgt 60tggctcccgt
at
7287771DNAArtificial Sequencechemically synthesized 877atacgggagc
caacaccatt gaggggctgg gatactgcaa cttaaagtcc aggagagcag 60gtgtgacgga t
7187871DNAArtificial Sequencechemically synthesized 878atccgtcaca
cctgctctcc tggactttaa gttgcagtat cccagcccct caatggtgtt 60ggctcccgta t
7187971DNAArtificial Sequencechemically synthesized 879atacgggagc
caacaccaat aagcgactag tgtattggta ctggcctttt ccgagagcag 60gtgtgacgga t
7188071DNAArtificial Sequencechemically synthesized 880atccgtcaca
cctgctctcg gaaaaggcca gtaccaatac actagtcgct tattggtgtt 60ggctcccgta t
7188172DNAArtificial Sequencechemically synthesized 881atacgggagc
caacaccaat cttactagtt tgggaaaaaa attaaatata agcaagagca 60ggtgtgacgg
at
7288272DNAArtificial Sequencechemically synthesized 882atccgtcaca
cctgctcttg cttatattta atttttttcc caaactagta agattggtgt 60tggctcccgt
at
7288372DNAArtificial Sequencechemically synthesized 883atacgggagc
caacaccagt ccgttatgac atgtccggac ccgtacgcgt gtcaagagca 60ggtgtgacgg
at
7288472DNAArtificial Sequencechemically synthesized 884atccgtcaca
cctgctcttg acacgcgtac gggtccggac atgtcataac ggactggtgt 60tggctcccgt
at
7288571DNAArtificial Sequencechemically synthesized 885atacgggagc
caacaccagg gattgcatta cccatgacta agagtaggtc gcaagagcag 60gtgtgacgga t
7188671DNAArtificial Sequencechemically synthesized 886atccgtcaca
cctgctcttg cgacctactc ttagtcatgg gtaatgcaat ccctggtgtt 60ggctcccgta t
7188772DNAArtificial Sequencechemically synthesized 887atacgggagc
caacaccata gaagtatgtt gttattctat ggaaataaaa cgacagagca 60ggtgtgacgg
at
7288872DNAArtificial Sequencechemically synthesized 888atccgtcaca
cctgctctgt cgttttattt ccatagaata acaacatact tctatggtgt 60tggctcccgt
at
7288972DNAArtificial Sequencechemically synthesized 889atacgggagc
caacaccatc ccgttgtgat cagagagcat gaaatgatgt tttgagagca 60ggtgtgacgg
at
7289072DNAArtificial Sequencechemically synthesized 890atccgtcaca
cctgctctca aaacatcatt tcatgctctc tgatcacaac gggatggtgt 60tggctcccgt
at
7289172DNAArtificial Sequencechemically synthesized 891atacgggagc
caacaccatg catgggacct gttatcctaa caagctgtca aggcagagca 60ggtgtgacgg
at
7289272DNAArtificial Sequencechemically synthesized 892atccgtcaca
cctgctctgc cttgacagct tgttaggata acaggtccca tgcatggtgt 60tggctcccgt
at
7289371DNAArtificial Sequencechemically synthesized 893atacgggagc
caacaccaca aaacgttccg agggagtaag cacttaataa tgtagagcag 60gtgtgacgga t
7189471DNAArtificial Sequencechemically synthesized 894atccgtcaca
cctgctctac attattaagt gcttactccc tcggaacgtt ttgtggtgtt 60ggctcccgta t
7189572DNAArtificial Sequencechemically synthesized 895atacgggagc
caacaccacg tcttatagat gtctgtattg tttatcgctc gcccagagca 60ggtgtgacgg
at
7289672DNAArtificial Sequencechemically synthesized 896atccgtcaca
cctgctctgg gcgagcgata aacaatacag acatctataa gacgtggtgt 60tggctcccgt
at
7289772DNAArtificial Sequencechemically synthesized 897atacgggagc
caacaccacc atctctggtg ataaccagtg atcttaacta tagcagagca 60ggtgtgacgg
at
7289872DNAArtificial Sequencechemically synthesized 898atccgtcaca
cctgctctgc tatagttaag atcactggtt atcaccagag atggtggtgt 60tggctcccgt
at
7289972DNAArtificial Sequencechemically synthesized 899atacgggagc
caacaccacc acctcactac agtgatcttt tgctctgaat agccagagca 60ggtgtgacgg
at
7290072DNAArtificial Sequencechemically synthesized 900atccgtcaca
cctgctctgg ctattcagag caaaagatca ctgtagtgag gtggtggtgt 60tggctcccgt
at
7290172DNAArtificial Sequencechemically synthesized 901atacgggagc
caacaccatg tctcttagga tacaaagcca aactgagccc gtgcagagca 60ggtgtgacgg
at
7290272DNAArtificial Sequencechemically synthesized 902atccgtcaca
cctgctctgc acgggctcag tttggctttg tatcctaaga gacatggtgt 60tggctcccgt
at
7290372DNAArtificial Sequencechemically synthesized 903atacgggagc
caacaccacc tccaatagcc aaaagaaatc gccaactaac ggcaagagca 60ggtgtgacgg
at
7290472DNAArtificial Sequencechemically synthesized 904atccgtcaca
cctgctcttg ccgttagttg gcgatttctt ttggctattg gaggtggtgt 60tggctcccgt
at
7290572DNAArtificial Sequencechemically synthesized 905atacgggagc
caacaccatc actactttta taatttcatt cttctggcgt ccctagagca 60ggtgtgacgg
at
7290672DNAArtificial Sequencechemically synthesized 906atccgtcaca
cctgctctag ggacgccaga agaatgaaat tataaaagta gtgatggtgt 60tggctcccgt
at 72
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