COMPOSITIONS AND METHODS FOR IMPROVING POD SHATTER TOLERANCE IN CANOLA

Abstract

Genome edited plants, plant cells, seeds and plant parts of Brassica are provided where expression levels and/or activities of pod dehiscence genes are modulated to improve one or more agronomic characteristics such as pod shatter. Also provided are compositions comprising polynucleotides encoding polypeptides and guide RNAs targeted to endogenous Brassica proteins involved in pod dehiscence including for example, targeted site-directed mutagenesis using CRISPR-associated nucleases. Additionally, various methods of employing the polynucleotides and genetic modifications in plants, such as methods for modulating expression level in a Brassica plant and methods for increasing pod shatter tolerance of a Brassica plant are also provided herein.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This Application claims priority from, and benefit of, U.S. Provisional Application 62/863,551 filed on June 19, 2019. The entire contents of the provisional application are hereby incorporated by reference herein.

REFERENCE TO SEQUENCE LISTING SUBMITTED ELECTRONICALLY

[0002] The official copy of the sequence listing is submitted electronically via EFS-Web as an ASCII formatted sequence listing with a file named 8043WOPCT_ST25.txt created on Jun. 13, 2020 and having a size of 368 kilobytes and is filed concurrently with the specification. The sequence listing comprised in this ASCII formatted document is part of the specification and is herein incorporated by reference in its entirety.

FIELD

[0003] This disclosure relates to compositions and methods for improving agronomic traits in plants, specifically improving pod shatter tolerance in Brassica plants.

BACKGROUND

[0004] Brassica napus (also referred to as canola or oilseed rape) is one of the most important vegetable oilseed crops in the world, especially in China, Canada, the European Union and Australia, where the oils are used extensively in the food industry and for biodiesel production. Oilseed rape is a recently domesticated plant and retains some of the traits of its wild ancestors which were useful in the wild but are not useful in commercial crop plants. One example of such a trait is fruit dehiscence, which refers to the natural opening of reproductive structures to disperse seeds. In species that disperse their fruit through dehiscense, siliques or pods are composed of two carpels that are held together by a central replum via a valve margin. Where the valve margin connects to the replum is called the dehiscence zone (DZ). When the pod is ripe, the valve margin detaches from the replum and the pod splits open, releasing the seeds inside. The DZ demarcates the precise location where the valves detach.

[0005] During crop domestication, farmers and breeders have selected for Brassica plants that avoid releasing their seeds early, before the crop is harvested. However, such early pod dehiscence (also known as "pod shatter", "seed shatter" or "seed shedding") has not been fully eliminated. Therefore, B. napus plants remain prone to seed losses due to pod shatter prior to harvest. Pod shatter poses significant problems for commercial production of canola seeds and adverse weather conditions can exacerbate the process resulting in an increase in shatter-related losses of 25% or more. This loss of seed not only has a dramatic effect on yield but can also result in the emergence of the crop as a weed in the subsequent growing season.

[0006] In addition to direct losses of income from reduced seed yield, increased input costs and reduced price paid for low oil content seeds, pod shatter also results in additional indirect costs to the grower. The shed seed results in self-sown or volunteer B. napus plants growing in the next year's crop, which creates further expense due to the need for increased herbicide use. Such self-sown B. napus plants cause losses due to competition with subsequent crop and can cause problems for farmers using reduced-tillage strategies such as no-till, zone-till, and strip tillage.

[0007] Resistance to pod shatter (indehiscent phenotype) is a key trait that has been selected during crop domestication. Plants have been also generated using Ethyl Methane Sulfonate (EMS) mutagenesis or through single guide gene editing. Rajani and Sundaresan, 2001, Current Biology, 11(24), 1914-1922; Liljegren et al., 2004, Cell, 116(6), 843-853; Braatz et al., 2017, Plant Physiology, 174(2), 935-942; Braatz et al., 2018, Euphytica, 214(2), 29; Braatz et al., 2018, Theor. Applied Genetics, 131(4), 959-971. However, some of these approaches have produced plants with "huge background mutations" and plants that are otherwise "unsuitable for agronomic purposes" (Zhai et al., 2019, Theor. Applied Genetics, 132: 2111-2123 at 2112 and 2121). Thus, there remain varieties of B. napus that are still dehiscent and prone to pod shatter. In view of the foregoing, there is a need for more B. napus lines having a pod shatter tolerance, i.e., indehiscent phenotype, and new approaches for generating such plants. There is also a need for pod-shatter phenotypes that permit plant seeds to be collected at harvest by threshing pods, e.g., using a combine harvester, with minimal damage to the seed.

SUMMARY

[0008] Brassica napus (2n =38) is an amphidiploid species that originated from the spontaneous hybridization between B. oleracea (2n =18) and B. rapa (2n =20). The B. napus genome comprises an A and a C genome.

[0009] Provided herein are methods for introducing a targeted genomic modification in a B. napus plant that contributes to an increasing pod shatter tolerance phenotype in the plant or in progeny from the plant. Generally, the method includes introducing a targeted modification into the genome of a B. napus plant, plant cell, or seed thereof, wherein the targeted modification includes excising endogenous genomic sequence of an INDEHISCENT (BnIND) gene, ALCATRAZ (BnALC) gene, POLYGALACTURONASE (BnPGAZ) gene, or any combination thereof. Each of these excisions or targeted deletions of genomic sequence is referred to herein as a "dropout" that inactivates the targeted gene.

[0010] In one aspect, the disclosure provides a method for increasing pod shatter tolerance in a B. napus plant (or in the plant's progeny) by introducing a targeted genomic modification in a B. napus plant, plant cell, or seed thereof. The disclosure also provides the modified B. napus plant, plant cell, or seed thereof, which is produced by the disclosed method. Thus a modified B. napus plant, plant cell, or seed thereof includes a targeted genomic modification that is a deletion or dropout of at least one allele of the following genes: the A genome BnIND-A (e.g., gene encoding SEQ ID NO:1, 2 or 3), C genome BnIND-C (e.g., gene encoding SEQ ID No:5, 6, or 7), A genome BnALC-A (e.g., gene encoding SEQ ID No:16, 17, or 18), C genome BnALC-C (e.g., gene encoding SEQ ID No:19, 20, or 21), A genome BnPGAZ-A (e.g., gene encoding SEQ ID NO:8, 9, 10, or 11), or C genome BnPGAZ-C gene (e.g., gene encoding SEQ ID NO:12, 13, 14, or 15). For example, the method can include introducing a dropout of at least one allele of BnIND-A (SEQ ID NO:56, 69, 105, 106, or 107); BnIND-C (SEQ ID NO:57, 67, 70, 108, 109, 110, or 111); BnALC-A (SEQ ID No:71, 73, 120, 122, or 124); BnALC-C (SEQ ID No:72, 74, 121, 123, or 125); BnPGAZ-A (SEQ ID No:60, 112, 113, 114, 115, 126, or 128); or BnPGAZ-C (SEQ ID No:61, 116, 117, 118, 119, 127, or 128). The method can further include introducing one or more combinations of the foregoing targeted dropouts. Thus, the modified B. napus plant, plant cell, or seed thereof can comprise one, two, three or four excised alleles of the BnIND gene; one, two, three or four excised alleles of the BnALC gene; or one, two, three or four excised alleles of the BnPGAZ gene.

[0011] In some examples, the modified B. napus plant is homozygous for gene edited deletions and includes a targeted dropout at both alleles of the BnIND-A gene, BnIND-C gene, BnALC-A gene, BnALC-C gene, BnPGAZ-A gene, BnPGAZ-C gene, or any combination of the foregoing. In other examples, the modified B. napus plant is heterozygous for the dropout and includes a dropout at only a single allele of the BnIND-A gene, BnIND-C gene, BnALC-A gene, BnALC-C gene, BnPGAZ-A gene, BnPGAZ-C gene, or any combination of the foregoing. In still other examples, the modified B. napus plant combines heterozygous and homozygous targeted excisions at BnIND-A gene, BnIND-C gene, BnALC-A gene, BnALC-C gene, BnPGAZ-A gene, or BnPGAZ-C gene. Thus, a modified B. napus plant can have one or a combination of the genotypes shown in Table 1 (wherein, in accordance with convention, superscript+indicates" wildtype allele and-indicates an allele modified by targeted excision of endogenous genomic sequence).

TABLE-US-00001 TABLE 1 W X Y Z 1 BnIND-A.sup.+/- BnIND-A.sup.-/-- BnIND-C.sup.+/- BnIND-C.sup.-/- 2 BnALC-A.sup.+/- BnALC-A.sup.-/- BnALC-C.sup.+/- BnALC-C.sup.-/- 3 BnPGAZ-A.sup.+/- BnPGAZ-A.sup.-/- BnPGAZ-C.sup.+/- BnPGAZ-C.sup.-/-

[0012] In a preferred aspect, the modified B. napus plant comprises three or four excised alleles of the BnIND gene identified by (row number column header) in Table 1: (1W, 1Z); (1X, 1Y); (1X, 1Y); or (1X, 1Z). In another preferred aspect, the modified B. napus plant comprises three or four excised alleles of the BnALC gene identified by reference to Table 1: (2W, 2Z); (2X, 2Y); (2X, 2Y); or (2X, 2Z); or three or four excised alleles of the BnPGAZ gene identified by reference to Table 1: (3W, 3Z); (3X, 3Y); (3X, 3Y); or (3X, 3Z).

[0013] Modified B. napus plant can include dropout genotypic combinations identified by reference to Table 1 (row number column header): (1W, 2W); (2W, 3W); (1W, 3W); (1W, 2W, 3W); (1X, 2X); (2X, 3X); (1X, 3X); (1X, 2X, 3X); (1Y, 2Y); (2Y, 3Y); (1Y, 3Y); (1Y, 2Y, 3Y); (1Z, 2Z); (2Z, 3Z); (1Z, 3Z); (1Z, 2Z, 3Z); (1W, 2X); (1W, 3X); (1W, 1X); (2W, 1X); (2W, 3X); (2W, 2X); (3W, 2X); (3W, 1X); (3W, 3X); (1Y, 2W, 3W); (1W, 2Y, 3W); (1W, 2W, 3Y); (1Z, 2W, 3W); (1W, 2Z, 3W); (1W, 2W, 3Z); (1X, 2W, 3W); (1W, 2X, 3W); (1W, 2W, 3X); (1W, 2X, 3X); (1X, 2W, 3X); (1X, 2X, 3W); (1W, 2Y, 3Y); (1Y, 2W, 3Y); (1Y, 2Y, 3W); (1X, 2Y, 3Y); (1Y, 2X, 3Y); (1Y, 2Y, 3X); (1X, 2Z, 3Z); (1Z, 2X, 3Z); (1Z, 2Z, 3X); (1W, 2X, 3Y); (1Y, 2X, 3W); (1X, 2W, 3Y); (1Y, 2W, 3X); (1X, 2Y, 3W); (1W, 2Y, 3X); (1W, 2Z, 3Y); (1Y, 2Z, 3W); (1Z, 2W, 3Y); (1Y, 2W, 3Z); (1Z, 2Y, 3W); (1W, 2Y, 3Z); (1Z, 2X, 3Y); (1Y, 2X, 3Z); (1X, 2Z, 3Y); (1Y, 2Z, 3X); (1X, 2Y, 3Z); (1Z, 2Y, 3X); (1W, 2X, 3Z); (1Z, 2X, 3W); (1X, 2W, 3Z); (1Z, 2W, 3X); (1X, 2Z, 3W); (1W, 2Z, 3X); (1X, 1Y); (1X, 1Z); (1X, 2Y); (1X, 2Z); (1X, 3Y); (1X, 3Z); (1W, 1Y); (1W, 1Z); (1W, 2Y); (1W, 2Z); (1W, 3Y); (1W, 3Z); (2X, 1Y); (2X, 1Z); (2X, 2Y); (2X, 2Z); (2X, 3Y); (2X, 3Z); (2W, 1Y); (2W, 1Z); (2W, 2Y); (2W, 2Z); (2W, 3Y); (2W, 3Z); (3X, 1Y); (3X, 1Z); (3X, 2Y); (3X, 2Z); (3X, 3Y); (3X, 3Z); (3W, 1Y); (3W, 1Z); (3W, 2Y); (3W, 2Z); (3W, 3Y); or (3W, 3Z).

[0014] Methods for generating the foregoing targeted modifications can include inducing double strand breaks using a TALE-nuclease (TALEN), a meganuclease, a zinc finger nuclease, or a CRISPR-associated nuclease. In a preferred aspect, the method includes introducing a CRISPR-associated nuclease and guide RNAs into a B. napus plant cell to generate one or more of the excised alleles or dropouts identified in Table 1.

[0015] The disclosure also provides a first and a second guide RNAs, which can be used in the disclosed CRISPR method. Exemplary guide RNAs can include any of the foregoing 1) a first guide RNA comprising SEQ ID NO:26 and a second guide RNA comprising SEQ ID NO:27 that catalyze targeted deletion of endogenous genomic BnIND sequence in the plant cell; 2) a first guide RNA comprising SEQ ID NO:28 and a second guide RNA comprising SEQ ID NO:29 that catalyze targeted deletion of endogenous genomic BnPGAZ sequence in the plant cell; 3) a first guide RNA comprising SEQ ID NO:30 and a second guide RNA comprising SEQ ID NO:29 that catalyze targeted deletion of endogenous genomic BnPGAZ sequence in the plant cell; 4) a first guide RNA comprising SEQ ID NO:31 and a second guide RNA comprising SEQ ID NO:32 that catalyze targeted deletion of endogenous genomic BnALC sequence in the plant cell; 5) a first guide RNA comprising SEQ ID NO:33 and a second guide RNA comprising SEQ ID NO:34 that catalyze targeted deletion of endogenous genomic BnALC sequence in the plant cell; or 6) a first guide RNA comprising SEQ ID NO:35 and a second guide RNA comprising SEQ ID NO:33 that catalyze targeted deletion of endogenous genomic BnALC sequence in the plant cell. In an aspect of the disclosure, the guide RNA is at least 99% identical to sequence encoding an amino acid sequence selected from the group consisting of SEQ ID NOs: 1-21.

[0016] Also provided herein is a modified B. napus plant, seed, or plant cell comprising a gene-edited deletion that removes one or more of the following genomic segments: (i) in BnIND-A the genomic segment corresponding to the sequence from position 7740 to position 10346 of SEQ ID NO:56 or the genomic segment corresponding to the sequence from position 2018 to position 4639 of SEQ ID NO:69; (ii) in BnIND-C the genomic segment corresponding to the sequence from position 2676 to position 5101 of SEQ ID NO:57, the genomic segment corresponding to the sequence from position 2019 to position 4441 of SEQ ID NO:67, or the genomic segment corresponding to the sequence from position 2018 to position 4446 of SEQ ID NO:70; (iii) in BnALC-A the genomic segment corresponding to the sequence from position 1723 to position 2849 of SEQ ID NO:71, the genomic segment corresponding to the sequence from position 1722 to position 2851 of SEQ ID NO:73, the genomic segment corresponding to the sequence from position 6369 to position 7511 of SEQ ID NO:72; (iv) in BnALC-C the genomic segment corresponding to the sequence from position 6368 to position 7510 of SEQ ID NO:74, the genomic segment corresponding to the sequence from position 3417 to position 6368 of SEQ ID NO:72, or the genomic segment from corresponding to position 3416 to position 6367 of SEQ ID NO:74; (v) in BnPGAZ-A the genomic segment corresponding to position 2720 to position 5866 of SEQ ID NO:60, the genomic segment corresponding to position 2720 to position 5865 of SEQ ID NO:60, the genomic segment corresponding to position 2015 to position 5235 of SEQ ID NO:126, the genomic segment corresponding to position 2015 to position 5231 of SEQ ID NO:126, the genomic segment corresponding to position 2019 to position 5235 of SEQ ID NO:128, or the genomic segment corresponding to position 2016 to position 5249 of SEQ ID NO:128; (vi) in BnPGAZ-C the genomic segment corresponding to position 2705 to position 5804 of SEQ ID NO:61, the genomic segment corresponding to position 2704 to position 5802 of SEQ ID NO:61, the genomic segment corresponding to position 2705 to position 5802 of SEQ ID NO:61, the genomic segment corresponding to position 2019 to position 5167 of SEQ ID NO:127, or the genomic segment corresponding to position 2023 to position 5192 of SEQ ID NO:129.

[0017] A modified B. napus plant can be generated from the modified B. napus plant cell or seed disclosed herein that comprises one or more allele of the gene-edited deletion of native BnIND sequence, native BnALC sequence, or native BnPGAZ sequence disclosed in the foregoing paragraph. The modified B. napus plant can comprise one, two, three or four excised alleles of the BnIND gene; one, two, three or four excised alleles of the BnALC gene; or one, two, three or four excised alleles of the BnPGAZ gene. For example, the modified B. napus plant can comprise three or four alleles of the gene-edited deletion of native BnIND disclosed in the foregoing paragraph that corresponds to the combination of alleles identified in Table 1 (row number column header): (1W, 1Z); (1X, 1Y): (1X, 1Y); or (1X, 1Z). In another example, the modified B. napus can comprise three or four alleles of the gene-edited deletion of native BnALC disclosed in the foregoing paragraph that can be described by reference to Table 1: (2W, 2Z); (2X, 2Y): (2X, 2Y); or (2X, 2Z). In yet another example, the modified B. napus plant can comprise three or four alleles of the gene-edited deletion of native BnPGAZ gene disclosed in the foregoing paragraph that corresponds that can be described by reference to Table 1: (3W, 3Z); (3X, 3Y): (3X, 3Y); or (3X, 3Z).

[0018] For example, the modified B. napus plants, plant cell or seed thereof can comprise one or more of the following genomic deletion or dropout sequences. BnIND dropout sequence can comprise SEQ ID NOs:58, 59, 68, 75, or 76. BnPGAZ dropout sequence can comprise SEQ ID NOs:62, 63, 64, 65, 66, 77, 78, 79, or 80. Accordingly, a B. napus plants, plant cell or seed thereof having a genomic modification that contributes to pod shatter tolerance can be identified and selected for using a method that includes isolating genomic DNA, optionally amplifying the genomic DNA, performing DNA sequencing. The presence of SEQ ID NO:58, 59, 68, 75, or 76 in the sequence indicates the B. napus plant, plant cell or seed thereof comprises a BnIND dropout that contributes to pod-shatter tolerance. The presence of a gene-edited deletion of BnALC-A genomic sequence corresponding to position 1723 to position 2849 of SEQ ID NO:71, BnALC-A genomic sequence corresponding to position 1722 to position 2851 of SEQ ID NO:73, BnALC-C genomic sequence corresponding to position 6369 to position 7511 of SEQ ID NO:72, BnALC-C genomic sequence corresponding to position 6368 to position 7510 of SEQ ID NO:74, BnALC-C genomic sequence corresponding to position 3417 to position 6368 of SEQ ID NO:72, or BnALC-C genomic sequence corresponding to position 3416 to position 6367 of SEQ ID NO:74 indicates the B. napus plant, plant cell or seed thereof comprises a BnALC dropout that contributes to pod-shatter tolerance. The presence of SEQ ID NOs:62, 63, 64, 65, 66, 77, 78, 79, or 80 in the sequence indicates the B. napus plant, plant cell or seed thereof comprises a BnPGAZ dropout that contributes to pod-shatter tolerance.

[0019] The modified B. napus plants disclosed herein are characterized by having increased pod shatter tolerant phenotype relative to an unmodified isogenic B. napus plant lacking the gene-edited deletion of BnIND gene, BnALC gene, or BnPGAZ gene disclosed herein. Alternatively, the modified B. napus plants can be used to generate, e.g., by breeding, a B. napus plant seed with increased pod shatter tolerance. Thus, when the modified B. napus plant disclosed herein is used as a first parent plant for breeding with a second parent B. napus plant, the gene-edited deletion of BnIND gene, BnALC gene, BnPGAZ, or a combination thereof can contribute to the pod shatter tolerant phenotype of resulting progeny. Such progeny plant can have increased pod shatter tolerance relative to alternative progeny produced using an unmodified, isogenic B. napus plant lacking the gene-edited deletion (instead of the first parent plant) in a breeding pair with the second parent plant.

[0020] Also provided herein is a method of introducing a natural deletion of the BnIND-A gene into a modified B. napus plant. The method includes crossing a B. napus plant comprising a native BnIND-A deletion with a modified B. napus parent plant disclosed herein having one, two, or three or four excised alleles of the BnIND, BnALC, or BnALC gene disclosed herein. For example, the modified parent plant can comprise three or four excised alleles of the BnIND gene identified by (row number column header) in Table 1: (1W, 1Z); (1X, 1Y): (1X, 1Y); or (1X, 1Z), (ii) three or four excised alleles of the BnALC gene identified by reference to Table 1: (2W, 2Z); (2X, 2Y): (2X, 2Y); or (2X, 2Z), or (iii) three or four excised alleles of the BnALC gene identified by reference to Table 1: (3W, 3Z); (3X, 3Y): (3X, 3Y); or (3X, 3Z). The cross produces hybrid progeny plants having the natural deletion of the BnIND-A gene and the one or more gene-edited targeted deletion of a BnIND, BnALC, or BnPGAZ gene allele of the modified parent plant. The method can further include selecting for one or more progeny plant(s) having both the natural deletion of the BnIND-A gene and at least one gene-edited targeted deletion of a BnIND, BnALC, or BnPGAZ gene disclosed herein. In a further aspect, the one or more progeny plant(s) can be (a) crossed with the modified B. napus parent plant to produce backcross progeny plants and (b) selecting backcross progeny plants that have the natural deletion of the BnIND-A gene and the one or targeted dropout of a BnIND, BnALC, or BnPGAZ gene disclosed herein. Further backcrossing includes using the selected backcross progeny plants to repeat steps (a) and (b) at least three or more times to produce further backcrossed progeny plants that comprise the natural deletion of the BnIND-A gene and the higher fraction of genetic material from the modified B. napus parent plant. This higher fraction of genetic material results in further backcrossed progeny plants having comparable or the same agronomic properties as the modified B. napus parent plant when grown in the same environmental conditions.

[0021] In preferred embodiments, each of the modified B. napus plant disclosed herein containing one or more targeted dropouts of a BnIND, BnALC, or BnPGAZ allele have suitable agronomic properties for commercial crop use.

[0022] Additionally, the disclosure provides Arabidopsis plants that comprise targeted dropouts of one or more alleles of the AtIND gene, AtALC gene, or a combination thereof.

[0023] The compositions and methods provided by this disclosure can be more fully understood from the following detailed description and the accompanying drawings and Sequence Listing, which form a part of this application.

BRIEF DESCRIPTION OF THE DRAWINGS AND SEQUENCE LISTING

[0024] FIG. 1 is a genomic map of AtIND Chr4:40000.45000 annotated to show CRISPR guide RNAs.

[0025] FIG. 2: is a genomic map of AtALC Chr5:2678000-2679100 annotated to show CRISPR guide RNAs.

[0026] FIG. 3: is a genomic map of IND-A and IND-C loci, which is annotated to show the position of target and cut sites of BNA-IND-CR1 and BNA-IND-CR2 in the A and C genomes of B. napus line NS1822BC. Primers used for genotyping using amplicon DNA sequencing are also indicated.

[0027] FIG. 4: is a genomic map of IND-C locus of B. napus line G00010BC annotated to show the guide RNA target sites and genotyping primers.

[0028] FIG. 5 is a genomic map of IND-A and IND-C loci B. napus line G00555MC annotated to show guide RNA target sites and genotyping primers.

[0029] FIG. 6 is a schematic illustrating a KASPAR.TM.assay designed to detect a BnIND-A natural deletion on chromosome NO3 (SEQ ID NO: 130). In FIG. 6, "1" indicates a wildtype allele-specific forward primer (e.g. SEQ ID NO:131); "2" indicates a wildtype specific common or reverse primer (e.g. SEQ ID NO:132), "3" indicates a natural deletion allele-specific forward primer (e.g. SEQ ID NO:133), and "4" indicates a natural deletion allele-specific common or reverse primer (e.g. SEQ ID NO:134).

[0030] FIG. 7 is a schematic illustrating a TAQMAN.TM. assay design to detect a BnIND-A natural deletion on chromosome NO3. In FIG. 7, "star 1" indicates a wildtype specific probe (e.g. SEQ ID NO:137; "star 2" indicates a natural deletion specific probe (e.g. SEQ ID NO:139); "3" indicates a wildtype and mutant common forward primer (e.g. SEQ ID NO:135), "4" indicates a wildtype allele-specific reverse primer (e.g. SEQ ID NO:136); and "5" indicates a natural deletion allele-specific reverse primer (e.g. SEQ ID NO:138).

[0031] FIG. 8 is a bar graph showing average percentage shattered pods of B. napus inbreds G00010BC, NS1822BC, and G00555MC, which were evaluated using a method for laboratory phenotyping pod shatter tolerance as disclosed herein.

[0032] FIG. 9 is a bar graph showing the average percentage shattered pods of G00010BC plants segregating for an IND-C dropout determined using a laboratory method for phenotyping pod shatter tolerance. Asterisk indicates a significant difference (T-test, p<0.05) as compared to wild-type (WT) plants. N=number of 15 pods replications for each zygosity category.

[0033] FIG. 10 is a bar graph showing the average percentage shattered pods of NS1822BC plants having indicated gene edited deletions. Pod shatter tolerance was determined using a laboratory phenotyping method. Hom refers to plants homozygous for the indicated gene edited deletion locus (IND-A or IND-C), Het refers to plants heterozygous for the indicated gene edited deletion locus (IND-A or IND-C), WT indicates unmodified NS1822BC and Double Het refers plants heterozygous at both IND-A or IND-C loci. Asterisk indicates a significant difference (T-test, p<0.05) as compared to WT plants.

[0034] FIG. 11 is a bar graph showing the average percentage of shattered pods of a commercial pod-shatter tolerant line (PST Check 1), G00010BC plants (2 KO), and modified G00010BC plants that are either homozygous (4 KO) or heterozygous (3 KO) for gene-edited deletions of IND-C gene allele. Pod shatter tolerance was determined using a laboratory phenotyping method.

[0035] FIG. 12 is a bar graph showing the average percentage of shattered pods (SHTPC) of B. napus plants homozygous or heterozygous, as indicated, for targeted deletions of IND-A or/and IND-C as compared to wildtype unmodified NS1822BC plants (WT). Single asterisk indicates a significant difference (T-test, p<0.01) and double asterisk indicates a significant difference (T-test, p<0.05), each as compared to WT plants that were not transformed. Pod shatter tolerance was determined using a field phenotyping method.

[0036] FIG. 13 is a bar graph showing the SHTPC of WT untransformed, which are unmodified G00010BC plants (2K0), WT segregant (2K0), and G00010BC plants that are either homozygous (4 KO) or heterozygous (3 KO) for gene-edited deletions of IND-C gene allele. Pod shatter tolerance was determined using a field phenotyping method.

[0037] FIG. 14 is a bar graph showing the average percentage of shattered pods Shatter tolerance of G00555MC x G00010BC gene edited hybrids with indicated dropout allele combinations and hybrid checks calculated as average percent shattered pods +/-SE. Pod shatter tolerance was determined using a lab phenotyping method. A and C indicate functional IND-A and IND-C alleles, respectively and lower case a and c indicate deletions of IND-A and IND-C alleles, respectively. Single asterisk indicates a significant difference (T-test, p<0.05) and double asterisk indicates a significant difference (T-test, p<0.01).

[0038] FIG. 15 is a set of four genomic maps of B. napus ALC-A and ALC-C regions in G00010BC (10BC) and G00555MC (55MC), respectively.

[0039] FIG. 16 is a genomic map of the B. napus PGAZ-A and PGAZ-C loci indicating the position of the target and cut sites of BNA-PGAZ-CR1 and BNA-PGAZ-CR2 on the NS1822BC A and C genomes. Primers used for genotyping using amplicon NextGen DNA sequencing are also indicated.

[0040] Nucleic acid sequences listed in the accompanying sequence listing and referenced herein are shown using standard letter abbreviations for nucleotide bases. Only one strand of each nucleic acid sequence is shown, but the complementary strand is understood to be included by any reference to the displayed strand. Sequence listings are described in the following Table 2.

TABLE-US-00002 TABLE 2 SEQ ID NO Description SEQ ID NO: 1 BnIND A genome protein SEQ ID NO: 2 NS1822BC BnIND A genome protein SEQ ID NO: 3 G00555MC BnIND A genome protein SEQ ID NO: 4 BnIND C genome protein SEQ ID NO: 5 NS1822BC BnIND C genome protein SEQ ID NO: 6 G00010BC BnIND C genome protein SEQ ID NO: 7 G00555MC BnIND C genome protein SEQ ID NO: 8 BnPGAZ A genome protein SEQ ID NO: 9 NS1822BC BnPGAZ A genome protein SEQ ID NO: 10 G00010BC BnPGAZ A genome protein SEQ ID NO: 11 G00555MC BnPGAZ A genome protein SEQ ID NO: 12 BnPGAZ C genome protein SEQ ID NO: 13 NS1822BC BnPGAZ C genome protein SEQ ID NO: 14 G00010BCBnPGAZ C genome protein SEQ ID NO: 15 G00555MC BnPGAZ C genome protein SEQ ID NO: 16 BnALC A genome protein SEQ ID NO: 17 G00010BC BnALC A genome protein SEQ ID NO: 18 G00555MC BnALC A genome protein SEQ ID NO: 19 BnALC C genome protein SEQ ID NO: 20 G00010BC BnALC C genome protein SEQ ID NO: 21 G00555MC BnALC C genome protein SEQ ID NO: 22 G00010BC BnALC A genome promoter SEQ ID NO: 23 G00555MC BnALC A genome promoter SEQ ID NO: 24 G00010BC BnALC C genome promoter SEQ ID NO: 25 G00555MC BnALC C genome promoter SEQ ID NO: 26 BnIND guide RNA SEQ ID NO: 27 BnIND guide RNA SEQ ID NO: 28 BnPGAZ guide RNA SEQ ID NO: 29 BnPGAZ guide RNA SEQ ID NO: 30 BnPGAZ guide RNA SEQ ID NO: 31 BnALC A genome RNA guide SEQ ID NO: 32 BnALC A genome RNA guide SEQ ID NO: 33 BnALC C genome RNA guide SEQ ID NO: 34 BnALC C genome RNA guide SEQ ID NO: 35 BnALC C genome promoter RNA guide SEQ ID NO: 36 AtIND coding sequence SEQ ID NO: 37 AtIND guide RNA SEQ ID NO: 38 AtIND guide RNA SEQ ID NO: 39 AtIND guide RNA SEQ ID NO: 40 AtIND guide RNA SEQ ID NO: 41 AtIND guide RNA SEQ ID NO: 42 AtIND guide RNA SEQ ID NO: 43 AtALC coding sequence SEQ ID NO: 44 AtALC guide RNA SEQ ID NO: 45 AtALC guide RNA SEQ ID NO: 46 AtALC guide RNA SEQ ID NO: 47 AtALC guide RNA SEQ ID NO: 48 AtALC guide RNA SEQ ID NO: 49 AtALC guide RNA SEQ ID NO: 50 AtALC guide RNA targets promoter SEQ ID NO: 51 AtALC guide RNA targets promoter SEQ ID NO: 52 genotyping primer AtIND dropout SEQ ID NO: 53 genotyping primer AtIND dropout SEQ ID NO: 54 genotyping primer AtIND dropout SEQ ID NO: 55 genotyping primer AtIND dropout SEQ ID NO: 56 NS1822BC BnIND genomic seq A genome SEQ ID NO: 57 NS1822BC BnIND genomic seq C genome SEQ ID NO: 58 NS1822BC BnIND A genome dropout SEQ ID NO: 59 NS1822BC BnIND C genome dropout SEQ ID NO: 60 NS1822BC BnPGAZ genomic seq A genome SEQ ID NO: 61 NS1822BC BnPGAZ genomic seq C genome SEQ ID NO: 62 NS1822BC BnPGAZ A genome dropout 1 SEQ ID NO: 63 NS1822BC BnPGAZ A genome dropout 2 SEQ ID NO: 64 NS1822BC BnPGAZ C genome dropout 1 SEQ ID NO: 65 NS1822BC BnPGAZ C genome dropout 2 SEQ ID NO: 66 NS1822BC BnPGAZ C genome dropout 3 SEQ ID NO: 67 G00010BC BnIND genomic seq C genome SEQ ID NO: 68 G00010BC BnIND C genome dropout SEQ ID NO: 69 G00555MC BnIND genomic seq A genome SEQ ID NO: 70 G00555MC BnIND genomic seq C genome SEQ ID NO: 71 G00010BC BnALC genomic seq A genome SEQ ID NO: 72 G00010BC BnALC genomic seq C genome SEQ ID NO: 73 G00555MC BnALC genomic seq A genome SEQ ID NO: 74 G00555MC BnALC genomic seq C genome SEQ ID NO: 75 G00555MC BnIND A genome dropout SEQ ID NO: 76 G00555MC BnIND C genome dropout SEQ ID NO: 77 G00010BC BnPGAZ A genome dropout SEQ ID NO: 78 G00010BC BnPGAZ C genome dropout SEQ ID NO: 79 G00555MC BnPGAZ A genome dropout SEQ ID NO: 80 G00555MC BnPGAZ C genome dropout SEQ ID NO: 81 NS1822BC_C75 IND-C FAMProbe SEQ ID NO: 82 NS1822BC_C75 IND-C VICProbe SEQ ID NO: 83 NS1822BC_C75 IND-C FP SEQ ID NO: 84 NS1822BC_C75 IND-C RP SEQ ID NO: 85 NS1822BC_C75 IND-C FP2 SEQ ID NO: 86 NS1822BC_C75 IND-C RP2 SEQ ID NO: 87 NS1822BC_C7B IND-C FAMProbe SEQ ID NO: 88 NS1822BC_C7B IND-C VICProbe SEQ ID NO: 89 NS1822BC_C7B IND-C FP SEQ ID NO: 90 NS1822BC_C7B IND-C RP SEQ ID NO: 91 NS1822BC_C7B IND-C FP2 SEQ ID NO: 92 NS1822BC_C7B IND-C RP2 SEQ ID NO: 93 NS1822BC_C7M IND-A FAMProbe SEQ ID NO: 94 NS1822BC_C7M IND-A VICProbe SEQ ID NO: 95 NS1822BC_C7M IND-A FP SEQ ID NO: 96 NS1822BC_C7M IND-A RP SEQ ID NO: 97 NS1822BC_C7M IND-A FP2 SEQ ID NO: 98 NS1822BC_C7M IND-A RP2 SEQ ID NO: 99 NS1822BC_C7N IND-A FAMProbe SEQ ID NO: 100 NS1822BC_C7N IND-A VICProbe SEQ ID NO: 101 NS1822BC_C7N IND-A FP SEQ ID NO: 102 NS1822BC_C7N IND-A RP SEQ ID NO: 103 NS1822BC_C7N IND-A FP2 SEQ ID NO: 104 NS1822BC_C7N IND-A RP2 SEQ ID NO: 105 BnIND-A/BnaA03g27180D/LK031800.1 coding region SEQ ID NO: 106 BnIND-A/NS1822BC coding region SEQ ID NO: 107 BnIND-A/G00555MC coding region SEQ ID NO: 108 BnIND-C/BnaC03g32180D/LK031870.1 coding region SEQ ID NO: 109 BnIND-C/NS1822BC coding region SEQ ID NO: 110 BnIND-C/G00010BC coding region SEQ ID NO: 111 BnIND-C/G00555MC coding region SEQ ID NO: 112 BnPGAZ-A/BnaA04g24130D coding region SEQ ID NO: 113 BnPGAZ-A/NS1822BC coding region SEQ ID NO: 114 BnPGAZ-A/G00010BC coding region SEQ ID NO: 115 BnPGAZ-A/G00555MC coding region SEQ ID NO: 116 BnPGAZ-C/BnaC04g47880D coding region SEQ ID NO: 117 BnPGAZ-C/NS1822BC coding region SEQ ID NO: 118 BnPGAZ-C/G00010BC coding region SEQ ID NO: 119 BnPGAZ-C/G00555MC coding region SEQ ID NO: 120 BnALC-A/BnaA07g12110D coding region SEQ ID NO: 121 BnALC-C/BnaC07g16290D coding region SEQ ID NO: 122 BnALC-A/G00010BC coding region SEQ ID NO: 123 BnALC-C/G00010BC coding region SEQ ID NO: 124 BnALC-A/G00555MC coding region SEQ ID NO: 125 BnALC-C/G00555MC coding region SEQ ID NO: 126 G00010BC BnPGAZ genomic seq A genome SEQ ID NO: 127 G00010BC BnPGAZ genomic seq C genome SEQ ID NO: 128 G00555MC BnPGAZ genomic seq A genome SEQ ID NO: 129 G00555MC BnPGAZ genomic seq C genome SEQ ID NO: 130 G00010BC IND genomic seq A genome SEQ ID NO: 131 BnIND-A WT F Primer SEQ ID NO: 132 BnIND-A WT R Primer SEQ ID NO: 133 BnIND-A natural deletion Mutant F Primer SEQ ID NO: 134 BnIND-A natural deletion Mutant R Primer SEQ ID NO: 135 BnIND-A WT and mutant common F Primer SEQ ID NO: 136 BnIND-A WT R Primer SEQ ID NO: 137 BnIND-A WT Probe SEQ ID NO: 138 BnIND-A natural deletion Mutant R Primer SEQ ID NO: 139 BnIND-A natural deletion Mutant Probe SEQ ID NO: 140 G00010BC BnPGAZ A genome dropout 2 SEQ ID NO: 141 G00555MC BnPGAZ A genome dropout 2 SEQ ID NO: 142 IND_CR1-FP detection of BnIND dropout SEQ ID NO: 143 IND_CR1-RP detection of BnIND dropout SEQ ID NO: 144 IND_CR2-FP detection of BnIND dropout SEQ ID NO: 145 IND_CR2-RP detection of BnIND dropout

DETAILED DESCRIPTION

[0041] Seed yield of B. napus and related plants is limited by pod "dehiscence" a process that occurs late in fruit development whereby the pod, or "silique" is opened and the enclosed seeds released. Degradation and separation of cell walls along a discrete layer of cells dividing the two halves of the pod, termed the "dehiscence zone" result in separation of the two halves of the pod and release of the contained seeds. The dehiscence zone is a region of only one to three cells in width that extends along the entire length of the valve/replum boundary (Meakin and Roberts, 1990, Exp. Botany, 41:995-1002). As the cells in the dehiscence zone separate from one another, the valves detach from the replum, allowing seeds to be dispersed. Seed "shattering," whereby seeds are prematurely shed through dehiscence before the crop can be harvested, is a significant problem faced by commercial seed producers and represents a loss of income to the industry.

[0042] Described herein are modified Brassica napus and Arabidoposis thaliana plants that provide resistance to seed shattering, i.e., the modified plants are pod shatter tolerant or their genotypes contribute to pod shatter tolerance of their progeny. The disclosed modified B. napus plants include a targeted modification, i.e., a gene-edited excision or dropout of endogenous genomic sequence of an INDEHISCENT (BnIND), ALCATRAZ (BnALC), or POLYGALACTURONASE (BnPGAZ).

[0043] Methods for creating such plants are also disclosed. The method for generating dropouts comprises inducing a first and second double strand break in genomic DNA using a TALE-nuclease (TALEN), a meganuclease, a zinc finger nuclease, or a CRISPR-associated nuclease. In a preferred aspect, the method comprises introducing a CRISPR-associated nuclease and guide RNAs into a B. napus plant cell. A CRISPR associated nuclease can be a CRISPR-Cas9 and guide RNAs can be one or more pairs of guide RNAs disclosed in Table 2 herein.

[0044] In some examples, the modified B. napus plants disclosed herein are characterized by having increased pod shatter tolerant phenotype relative to the same plant prior to modification (the plant lacking the gene-edited dropout of BnIND gene, BnALC gene, or BnPGAZ gene disclosed herein). In other examples, the modified B. napus plants disclosed herein can be used to generate, e.g., by breeding, a B. napus plant that has increased pod shatter tolerance. The modified B. napus plant disclosed can be used as a first parent plant for breeding with a second parent B. napus plant to create progeny that includes the targeted dropout of BnIND gene, BnALC gene, BnPGAZ, or a combination thereof. The dropout contributes to an increased pod shatter tolerant phenotype of resulting progeny having the one or more targeted dropout, as compared to progeny lacking the one or more gene-edited dropouts of BnIND gene, BnALC gene, or BnPGAZ gene allele as disclosed herein.

Terms and Definitions

[0045] "Increased pod shatter tolerance" and "reduced seed shattering", as used herein, refers to a decreased seed shatter tendency and/or a delay in the timing of seed shattering, in particular until harvest, of Brassica plants, the fruits of which normally do not mature synchronously, but sequentially, so that some pods burst open and shatter their seeds before or during harvest.

[0046] The term "ALCATRAZ gene", "ALC gene", "ALCATRAZ allele" or "ALC allele" refers herein to a gene that can contribute to pod shatter resistance in B. napus and A. thaliana (e.g. the gene encoding SEQ ID No:16, 17, 18, 19, 20, or 21). ALC gene plays a role in cell separation during fruit dehiscence by promoting the differentiation of a cell layer that is the site of separation between the valves and the replum within the dehiscence zone. Examples of ALC gene sequences include BnALC-A (SEQ ID No:71, 73, 120, 122, or 124) and BnALC-C (SEQ ID No:72, 74, 121, 123, or 125).

[0047] The term "INDEHISCENT gene", "IND gene", "INDEHISCENT allele" or "IND allele" refers herein to a gene that can contribute to pod shatter resistance in B. napus and A. thaliana. IND encodes a member of an atypical class of eukaryotic bHLH proteins (e.g., SEQ ID NO:1, 2, 3, 4, 5, 6, or 7) and is required for seed dispersal. IND is involved in the differentiation of all three cell types required for fruit dehiscence and acts as the key regulator in a network that controls specification of the valve margin. Examples of IND gene sequences include BnIND-A (SEQ ID NO:56, 69, 105, 106, or 107) and BnIND-C (SEQ ID NO:57, 67, 70, 108, 109, 110, or 111).

[0048] The term "POLYGALACTURONASE gene", "PGAZ gene", "POLYGALACTURONASE allele" or "PGAZ allele" refers herein to "polygalacturonase expressed in abscission zone" gene. PGAZ is involved in pectin degradation and subsequent loss of cell cohesion (Hadfield and Bennet 1998, Plant physiology, 117(2), 337-343.). PGAZ expression increases during a number of developmental processes thought to involve cell wall breakdown, including silique shattering (Jenkins et al., 1996, Journal of Exp. Botany, 47(1), 111-115; Jenkins et al., 1999, Plant, Cell & Environment, 22(2), 159-167; Ferrandiz, 2002, Journal of Exp. Botany, 53(377), 2031-2038). Examples of PGAZ-encoded protein products include SEQ ID NO:8, 9, 10, 11, 12, 13, 14, or 15) and examples of PGAZ genes include BnPGAZ-A (SEQ ID No:60, 112, 113, 114, 115, 126, or 128) and BnPGAZ-C (SEQ ID NO:61, 116, 117, 118, 119, 127, or 128) of SEQ ID NO:105 and SEQ ID NO:108.

[0049] In connection with pod shatter phenotypes evaluated herein, "fully shattered pods" are those with both valves detached from the replum and all seeds dispersed. "Half shattered pods" are those with one valve fully or partially detached from the replum, seeds dispersed, though the second valve is still attached and all or some seeds remain between the attached valve and the septum. "Unshattered pods" have both valves attached to the replum and seeds are contained between both valves and the septum. For laboratory phenotyping, "percent shattered pods" or "SHTPC" is the number of fully shattered +half shattered pods/total number of pods*100. For field phenotyping, "half shattered" pods are not counted, so "percent shattered pods" or "SHTPC" is the number of fully shattered/total number of pods*100. SHTPC is compared to the wildtype after the shatter inducing treatment.

[0050] "Brassica" refers to any one of Brassica napus (AACC, 2n=38), Brassica juncea (AABB, 2n=36), Brassica carinata (BBCC, 2n=34), Brassica rapa (syn. B. campestris) (AA, 2n=20), Brassica oleracea (CC, 2n=18) or Brassica nigra (BB, 2n=16).

[0051] An "allele" is one of several alternative forms of a gene occupying a given locus on a chromosome. When all the alleles present at a given locus on a chromosome are the same, that plant is "homozygous" at that locus. If the alleles present at a given locus on a chromosome differ, that plant is "heterozygous" at that locus. In B. napus, a plant can be homozygous. wildtype for the IND gene in the A genome, but heterozygous mutant for the IND gene in the C genome.

[0052] An "amplicon" is amplified nucleic acid, e.g., a nucleic acid that is produced by amplifying a template nucleic acid by any available amplification method (e.g., PCR, LCR, transcription, or the like).

[0053] "Backcrossing" refers to the process whereby hybrid progeny are repeatedly crossed back to one of the parents. In a backcrossing scheme, the "donor" parent refers to the parental plant with the desired gene or locus to be introgressed. The "recipient" parent (used one or more times) or "recurrent" parent (used two or more times) refers to the parental plant into which the gene or locus is being introgressed.

[0054] "CRISPR" (Clustered Regularly Interspaced Short Palindromic Repeats) loci refers to certain genetic loci encoding components of DNA cleavage systems, for example, used by bacterial and archaeal cells to destroy foreign DNA (Horvath and Barrangou, 2010, Science 327: 167-170; International Application Publication WO2007/025097, published 1 Mar. 2007). A CRISPR locus can consist of a CRISPR array, comprising short direct repeats (CRISPR repeats) separated by short variable DNA sequences (called spacers), which can be flanked by diverse Cas (CRISPR-associated) genes.

[0055] The term "Cas protein" refers to a polypeptide encoded by a Cas (CRISPR-associated) gene. A Cas protein includes but is not limited to: a Cas9 protein, a Cpf1 (Cas12) protein, a C2c1 protein, a C2c2 protein, a C2c3 protein, Cas3, Cas3-HD, Cas 5, Cas7, Cas8, Cas10, or combinations or complexes of these. A Cas protein may be a "Cas endonuclease" or "Cas effector protein", that when in complex with a suitable polynucleotide component, is capable of recognizing, binding to, and optionally nicking or cleaving all or part of a specific polynucleotide target sequence. A Cas endonuclease described herein comprises one or more nuclease domains. The endonucleases of the disclosure may include those having one or more RuvC nuclease domains. A Cas protein is further defined as a functional fragment or functional variant of a native Cas protein, or a protein that shares at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity with a native Cas protein, and retains at least partial activity.

[0056] A "Cas endonuclease" may comprise domains that enable it to function as a double-strand-break-inducing agent. A "Cas endonuclease" may also comprise one or more modifications or mutations that abolish or reduce its ability to cleave a double-strand polynucleotide (dCas). In some aspects, the Cas endonuclease molecule may retain the ability to nick a single-strand polynucleotide (for example, a D10A mutation in a Cas9 endonuclease molecule) (nCas9).

[0057] "Gene" includes a nucleic acid fragment that expresses a functional molecule such as, but not limited to, a specific protein, including regulatory sequences preceding (5' non-coding sequences) and following (3' non-coding sequences) the coding sequence, as well as intervening intron sequences. "Native gene" refers to a gene as found in its natural endogenous location with its own regulatory sequences.

[0058] A "mutated gene" or "modified gene" is a gene that has been altered through human intervention. Such a "mutated" or "modified" gene has a sequence that differs from the sequence of the corresponding non-mutated gene by at least one nucleotide addition, deletion, or substitution. In certain embodiments of the disclosure, the mutated gene comprises an excision or deletion of a sequence of nucleotides within that results from two double strands break which are specifically targeted to a genomic sequence by guide polynucleotide/Cas endonuclease system as disclosed herein. A "mutated" or "modified" plant is a plant comprising a mutated gene or deletion.

[0059] As used herein, a "targeted mutation" is a mutation in a gene (referred to as the target gene), including a native gene, that was made by altering a target sequence within the target gene using any method known to one skilled in the art, including a method involving a guided Cas endonuclease system as disclosed herein.

[0060] The terms "dropout", "gene dropout", "knockout" and "gene knockout" refers to a DNA sequence of a cell (e.g. the BnIND gene) that has been excised from the genome by targeted deletion mediated by a Cas protein.

[0061] The term "genome" as it applies to a prokaryotic and eukaryotic cell or organism cells encompasses not only chromosomal DNA found within the nucleus, but organelle DNA found within subcellular components (e.g., mitochondria, or plastid) of the cell.

[0062] As used herein, a "genomic sequence" or "genomic region" is a segment of a chromosome in the genome of a cell that is present on either side of the target site or, alternatively, also comprises the target site or a portion thereof. An "endogenous genomic sequence" refers to genomic sequence within a plant cell, (e.g. an endogenous genomic sequence of an IND gene present within the genome of a Brassica plant cell).

[0063] A "genomic locus" as used herein refers to the genetic or physical location on a chromosome of a gene. As used herein, "gene" includes a nucleic acid fragment that expresses a functional molecule such as, but not limited to, a specific protein coding sequence and regulatory elements, such as those preceding (5' non-coding sequences) and following (3' non-coding sequences) the coding sequence.

[0064] As used herein, "genotype" is the actual nucleic acid sequence at one or more loci in an individual plant. As used herein, "phenotype" means the detectable characteristics (e.g. pod shatter tolerance) of a cell or organism which can be influenced by genotype.

[0065] As used herein, the term "guide polynucleotide", relates to a polynucleotide sequence that can form a complex with a Cas endonuclease, including the Cas endonuclease described herein, and enables the Cas endonuclease to recognize, optionally bind to, and optionally cleave a DNA target site. The guide polynucleotide sequence can be a RNA sequence, a DNA sequence, or a combination thereof (a RNA-DNA combination sequence).

[0066] The terms "single guide RNA" and "sgRNA" are used interchangeably herein and relate to a synthetic fusion of two RNA molecules, a crRNA (CRISPR RNA) comprising a variable targeting domain (linked to a tracr mate sequence that hybridizes to a tracrRNA), fused to a tracrRNA (trans-activating CRISPR RNA). The single guide RNA can comprise a crRNA or crRNA fragment and a tracrRNA or tracrRNA fragment of the type II CRISPR/Cas system that can form a complex with a type II Cas endonuclease, wherein said guide RNA/Cas endonuclease complex can direct the Cas endonuclease to a DNA target site, enabling the Cas endonuclease to recognize, optionally bind to, and optionally nick or cleave (introduce a single or double-strand break) the DNA target site.

[0067] As used herein, the terms "guide polynucleotide/Cas endonuclease complex", "guide polynucleotide/Cas endonuclease system", " guide polynucleotide/Cas complex", "guide polynucleotide/Cas system" and "guided Cas system" "Polynucleotide-guided endonuclease" , "PGEN" are used interchangeably herein and refer to at least one guide polynucleotide and at least one Cas endonuclease, that are capable of forming a complex, wherein said guide polynucleotide/Cas endonuclease complex can direct the Cas endonuclease to a DNA target site, enabling the Cas endonuclease to recognize, bind to, and optionally nick or cleave (introduce a single or double-strand break) the DNA target site. A guide polynucleotide/Cas endonuclease complex herein can comprise Cas protein(s) and suitable polynucleotide component(s) of any of the known CRISPR systems (Horvath and Barrangou, 2010, Science 327: 167-170; Makarova et al. 2015, Nature Reviews Microbiology Vol. 13: 1-15; Zetsche et al., 2015, Cell 163, 1-13; Shmakov et al., 2015, Molecular Cell 60, 1-13).

[0068] As used herein, a `nucleic acid molecule" is a polymeric form of nucleotides, which can include both sense and anti-sense strands of RNA, cDNA, genomic DNA, and synthetic forms and mixed polymers of the above. A nucleotide refers to a ribonucleotide, deoxynucleotide, or a modified form of either type of nucleotide. A "nucleic acid molecule" as used herein is synonymous with "nucleic acid", "nucleotide sequence", "nucleic acid sequence", and "polynucleotide." The term includes single- and double-stranded forms of DNA. A nucleic acid molecule can include either or both naturally occurring and modified nucleotides linked together by naturally occurring and/or non-naturally occurring nucleotide linkages.

[0069] Nucleic acid molecules may be modified chemically or biochemically, or may contain non-natural or derivatized nucleotide bases, as will be readily appreciated by those of skill in the art. Such modifications include, for example, labels, methylation, substitution of one or more of the naturally occurring nucleotides with an analog, internucleotide modifications, such as uncharged linkages (e.g., methyl phosphonates, phosphotriesters, phosphoramidates, carbamates, etc.), charged linkages (e.g., phosphorothioates, phosphorodithioates, etc.), pendent moieties (e.g., peptides), intercalators (e.g., acridine, psoralen, etc.), chelators, alkylators, and modified linkages (e.g., alpha anomeric nucleic acids, etc.). The term "nucleic acid molecule" also includes any topological conformation, including single-stranded, double-stranded, partially duplexed, triplexed, hairpinned, circular, and padlocked conformations. An "endogenous nucleic acid sequence" refers to a nucleic acid sequence within a plant cell, (e.g. an endogenous allele of an IND gene present within the genome of a Brassica plant cell).

[0070] A "protospacer adjacent motif" (PAM) herein refers to a short nucleotide sequence adjacent to a target sequence (protospacer) that is recognized (targeted) by a guide polynucleotide/Cas endonuclease system described herein. The Cas endonuclease may not successfully recognize a target DNA sequence if the target DNA sequence is not followed by a PAM sequence. The sequence and length of a PAM herein can differ depending on the Cas protein or Cas protein complex used. The PAM sequence can be of any length but is typically 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 nucleotides long.

[0071] As used herein, the term "plant material" refers to any processed or unprocessed material derived, in whole or in part, from a plant. For example, and without limitation, a plant material may be a plant part, a seed, a fruit, a leaf, a root, a plant tissue, a plant tissue culture, a plant explant, or a plant cell.

[0072] The terms "recombinant DNA molecule", "recombinant DNA construct", "expression construct", "construct", and "recombinant construct" are used interchangeably herein. A recombinant DNA construct comprises an artificial combination of nucleic acid sequences, e.g., regulatory and coding sequences that are not all found together in nature. For example, a recombinant DNA construct may comprise regulatory sequences and coding sequences that are derived from different sources, or regulatory sequences and coding sequences derived from the same source, but arranged in a manner different than that found in nature. In one aspect, a "construct" comprises a double-strand-break inducing agent (e.g. a Cas endonuclease and guide RNA complex). Such a construct may be used by itself or may be used in conjunction with a vector. If a vector is used, then the choice of vector is dependent upon the method that will be used to introduce the vector into the host cells. For example, a plasmid vector can be used that comprises the genetic elements needed to transform, select and propagate vector host cells. Different independent transformation events may result in different levels and patterns of expression (Jones et al., 1985, EMBO J4: 2411-2418; De Almeida et al., 1989, Mol Gen Genetics 218: 78-86), and thus multiple events are typically screened in order to obtain lines displaying the desired expression level and pattern. Such screening may be accomplished standard molecular biological, biochemical, and other assays including Southern analysis of DNA, Northern analysis of mRNA expression, PCR, real time quantitative PCR (qPCR), reverse transcription PCR (RT-PCR), immunoblotting analysis of protein expression, enzyme or activity assays, and/or phenotypic analysis.

[0073] The terms "target site", "target sequence", "target site sequence, "target DNA", "target locus", "genomic target site", "genomic target sequence", "genomic target locus", and "target polynucleotide", can be used interchangeably herein and refer to a polynucleotide sequence such as, but not limited to, a nucleotide sequence on a chromosome, episome, a locus, or any other DNA molecule in the genome (including chromosomal, chloroplastic, mitochondrial DNA, plasmid DNA) of a cell, at which a guide polynucleotide/Cas endonuclease complex can recognize, bind to, and optionally nick or cleave . The target site can be an endogenous site in the genome of a cell, or alternatively, the target site can be heterologous to the cell and thereby not be naturally occurring in the genome of the cell, or the target site can be found in a heterologous genomic location compared to where it occurs in nature. As used herein, terms "endogenous target sequence" and "native target sequence" are used interchangeable herein to refer to a target sequence that is endogenous or native to the genome of a cell and is at the endogenous or native position of that target sequence in the genome of the cell.

[0074] A virus or vector "transforms" or "transduces" a cell when it transfers nucleic acid molecules into the cell. A cell is "transformed" by a nucleic acid molecule transduced into the cell when the nucleic acid molecule becomes stably replicated by the cell, either by incorporation of the nucleic acid molecule into the cellular genome, or by episomal replication. As used herein, the term "transformation" encompasses all techniques by which a nucleic acid molecule can be introduced into such a cell. Examples include, but are not limited to, transfection with viral vectors, transformation with plasmid vectors, electroporation (Fromm et al., 1986, Nature 319: 791-3), lipofection (Feigner et al., 1987, Proc. Natl. Acad. Sci. USA 84: 7413-7), microinjection (Mueller et al., 1978, Cell 15: 579-85), Agrobacterium-mediated transfer (Fraley et al., 1983, Proc. Natl. Acad. Sci. USA 80: 4803-7), direct DNA uptake, and microprojectile bombardment (Klein et al., 1987, Nature 327: 70).

[0075] The phrase "under stringent conditions" refers to conditions under which a probe or polynucleotide will hybridize to a specific nucleic acid sequence, typically in a complex mixture of nucleic acids, but to essentially no other sequences. Stringent conditions are sequence-dependent and will be different in different circumstances.

[0076] "Variants" is intended to mean substantially similar sequences. For polynucleotides, a variant comprises a deletion and/or addition of one or more nucleotides at one or more internal sites within the native polynucleotide and/or a substitution of one or more nucleotides at one or more sites in the native polynucleotide (e.g. an IND dropout variant, with the chromosome region containing the IND gene excised). As used herein, a "native" polynucleotide or polypeptide comprises a naturally occurring nucleotide sequence or amino acid sequence, respectively.

[0077] "Variant" protein is intended to mean a protein derived from the native protein by deletion or addition of one or more amino acids at one or more internal sites in the native protein and/or substitution of one or more amino acids at one or more sites in the native protein.

III. Double-Strand-Break (DSB) Inducing Agents (DSB Agents)

[0078] Double-strand breaks can be induced by agents such as endonucleases that cleave the phosphodiester bond within a polynucleotide chain, can result in the induction of DNA repair mechanisms, including the non-homologous end-joining pathway, and homologous recombination. Endonucleases include a range of different enzymes, including restriction endonucleases (see e.g. Roberts et al., 2003 Nucleic Acids Res 1: 418-20, Roberts et al., 2003, Nucleic Acids Res 31: 1805-12, and Belfort et al., 2002 in Mobile DNA II, pp. 761-783, Eds. Craigie et al., (ASM Press, Washington, D.C.)), meganucleases (see e.g., International Application Publication WO 2009/114321; Gao et al., 2010, Plant Journal 1: 176-187), and TAL effector nucleases or TALENs (see e.g., US Application Publication US 20110145940 and Christian et al., 2010, Genetics 186(2): 757-61). Methods of targeting DNA double-strand breaks have been described for TALENs (Christian et al., 2010, Genetics 186(2): 757-61 and Boch et al., 2009, Science 326(5959): 1509-12), zinc finger nucleases (see e.g. Kim, et al., 1996, Proc. Nat'l Acad. Sci USA 93(3)1156-1160) and CRISPR-Cas endonucleases (see e.g. International Application Publication WO2007/025097).

[0079] Any DSB or -nick or -modification inducing agent may be used for the methods described herein, including for example but not limited to: Cas endonucleases, recombinases, TALENs, zinc finger nucleases, restriction endonucleases, meganucleases, and deaminases.

[0080] Methods and compositions are provided for polynucleotide modification with a CRISPR Associated (Cas) endonuclease. Class I Cas endonucleases comprise multi-subunit effector complexes (Types I, III, and IV), while Class 2 systems comprise single protein effectors (Types II, V, and VI) (Makarova et al., 2015, Nature Reviews Microbiology 13: 1-15; Zetsche et al., 2015, Cell 163: 1-13; Shmakov et al., 2015, Molecular Cell 60, 1-13; Haft et al., 2005, Computational Biology, PLoS Comput Biol (6): e60; and Koonin et al., 2017, Curr Opinion Microbiology 37: 67-78). In Class 2 Type II systems, the Cas endonuclease acts in complex with a guide RNA (gRNA) that directs the Cas endonuclease to cleave the DNA target to enable target recognition, binding, and cleavage by the Cas endonuclease. The gRNA comprises a Cas endonuclease recognition (CER) domain that interacts with the Cas endonuclease, and a Variable Targeting (VT) domain that hybridizes to a nucleotide sequence in a target DNA. In some aspects, the gRNA comprises a CRISPR RNA (crRNA) and a trans-activating CRISPR RNA (tracrRNA) to guide the Cas endonuclease to its DNA target. The crRNA comprises a spacer region complementary to one strand of the double strand DNA target and a region that base pairs with the tracrRNA, forming an RNA duplex. In many systems, the Cas endonuclease-guide polynucleotide complex recognizes a short nucleotide sequence adjacent to the target sequence (protospacer), called a "protospacer adjacent motif" (PAM).

[0081] Examples of a Cas endonuclease include but are not limited to Cas9 and Cpf1. Cas9 (formerly referred to as Cas5, Csn1, or Csx12) is a Class 2 Type II Cas endonuclease (Makarova et al., 2015, Nature Reviews Microbiology 13: 1-15). A Cas9-gRNA complex recognizes a 3' PAM sequence (NGG for the S. pyogenes Cas9) at the target site, permitting the spacer of the guide RNA to invade the double-stranded DNA target, and, if sufficient homology between the spacer and protospacer exists, generate a DSB cleavage. Cas9 endonucleases comprise RuvC and HNH domains that together produce DSBs, and separately can produce single strand breaks. For the S. pyogenes Cas9 endonuclease, the DSB leaves a blunt end. Cpf1 is a Class 2 Type V Cas endonuclease, and comprises nuclease RuvC domain but lacks an HNH domain (Yamane et al., 2016, Cell 165: 949-962). Cpf1 endonucleases create "sticky" overhang ends.

[0082] Some uses for Cas9-gRNA systems at a genomic target site include but are not limited to insertions, deletions, substitutions, or modifications of one or more nucleotides at the target site; modifying or replacing nucleotide sequences of interest (such as a regulatory elements); insertion of polynucleotides of interest; gene dropout; gene knock-out; gene knock in; modification of splicing sites and/or introducing alternate splicing sites; modifications of nucleotide sequences encoding a protein of interest; amino acid and/or protein fusions; and gene silencing by expressing an inverted repeat into a gene of interest. Genome editing using DSB-inducing agents, such as Cas9-gRNA complexes, has been described, for example in U.S. Patent Application US 2015-0082478 A1, published on March 19, 2015, International Application Publication WO2015/026886 A1, published on Feb. 26, 2015, WO2016007347, published on Jan. 14, 2016, and International Application Publication WO201625131, published on Feb. 18, 2016, all of which are incorporated by reference herein.

[0083] In some aspects of the disclosure, a targeted genomic modification is introduced in a B. napus plant cell, wherein the targeted modification includes excising endogenous genomic sequence of an INDEHISCENT (BnIND) gene, ALCATRAZ (BnALC), or POLYGALACTURONASE (BnPGAZ) in a B. napus plant cell. In a further aspect, the targeted genomic modification comprises first and second double strand breaks induced by a CRISPR-associated nuclease, Cas9. Cas9 is introduced into the B. napus cell with a first and second guide RNAs as Cas9-gRNA complexes that recognizes target sequences in the genome of the B. napus cell and is able to induce DSBs in the genomic sequence. In an aspect, the DSBs flank the endogenous target gene BnIND, BnALC, or BnPGAZ, allowing for the excision of the target gene.

[0084] IV. Recombinant Constructs and Transformation of Cells

[0085] The disclosed guide polynucleotides can be introduced into a cell with the disclosed CRISPR-Cas endonucleases. Cells include, but are not limited to, human, non-human, animal, bacterial, fungal, insect, yeast, non-conventional yeast, and plant cells as well as plants and seeds produced by the methods described herein. In a preferred aspect of the disclosure, the cells are B. napus cells.

[0086] Standard recombinant DNA and molecular cloning techniques used herein are well known in the art and are described more fully in Sambrook et al., Molecular Cloning: A Laboratory Manual; Cold Spring Harbor Laboratory: Cold Spring Harbor, N.Y. (1989). Transformation methods are well known to those skilled in the art and are described infra.

[0087] Vectors and constructs include circular plasmids, and linear polynucleotides, comprising a polynucleotide of interest and optionally other components including linkers, adapters, regulatory or analysis. In some examples a recognition site and/or target site can be comprised within an intron, coding sequence, 5' UTRs, 3' UTRs, and/or regulatory regions.

[0088] In one aspect, the constructs of the disclosure comprise a promoter operably linked to a nucleotide sequence encoding a Streptococcus pyrogenes Cas9 gene and a promoter operably linked to a guide RNA of the present disclosure. The promoter is capable of driving expression of an operably linked nucleotide sequence in a prokaryotic or eukaryotic cell/organism. In some aspects, target specific guide RNAs are built as a fusion of CRISPR RNA (crRNA) fused to trans-activating CRISPR RNA (tracrRNA) of Streptococcus pyrogenes.

[0089] In accordance with the methods disclosed herein, a first guide RNA comprising SEQ ID NO:26 and a second guide RNA comprising SEQ ID NO:27 can be used to catalyze targeted deletion of endogenous genomic BnIND sequence in the B. napus plant cell; a first guide RNA comprising SEQ ID NO:31 or SEQ ID NO:35 and a second guide RNA comprising SEQ ID NO:32 can be used to catalyze targeted deletion of endogenous genomic BnALC sequence in the B. napus plant cell; ; a first guide RNA comprising SEQ ID NO:33 and a second guide RNA comprising SEQ ID NO:34 can be used to catalyze targeted deletion of endogenous genomic BnALC sequence in the B. napus plant cell; and a first guide RNA comprising SEQ ID NO:28 or 30 and a second guide RNA comprising SEQ ID NO:29 can be used to catalyze targeted deletion of endogenous genomic BnPGAZ sequence in the B. napus plant cell.

[0090] The polynucleotides, constructs and vectors disclosed herein (e.g., for expression of endonucleases or guide RNAs) can comprise a selectable marker to identify or select for or against a molecule or a cell that comprises the construct or vector. Examples of selectable markers that can be used in a construct or vector disclosed herein include DsRed and Glyphosate N-Acetyltransferase (GAT) gene variant 4621 for herbicide resistance.

IV. Plant Transformation

[0091] Any of the techniques known in the art for introduction of transgenes into plants may be used to produce a transformed plant or plant cell disclosed herein. Suitable methods for transformation of plants are believed to include virtually any method by which DNA can be introduced into a cell, such as: by electroporation as illustrated in U.S. Pat. No. 5,384,253; by microprojectile bombardment, as illustrated in U.S. Pat. Nos. 5,015,580, 5,550,318, 5,538,880, 6,160,208, 6,399,861, and 6,403,865; by Agrobacterium-mediated transformation as illustrated in U.S. Pat. Nos. 5,635,055, 5,824,877, 5,591, 616; 5,981,840, and 6,384,301; and by protoplast transformation, as set forth in U.S. Pat. No. 5,508,184, etc. These techniques can be used to transform plant cells and these cells may be developed into transgenic plants by techniques known to those of skill in the art. Techniques for transforming Brassica plants in particular are disclosed, for example, in U.S. Pat. No. 5,750,871.

[0092] After effecting delivery of exogenous DNA to recipient cells, transformed cells are identified for further culturing and plant regeneration. In order to improve the ability to identify transformants, one may desire to employ a selectable marker gene with the transformation vector used to generate the transformant. In this case, the potentially transformed cell population can be assayed by exposing the cells to a selective agent or agents, or the cells can be screened for the desired marker.

[0093] Cells that survive the exposure to the selective agent, or cells that have been scored positive in a screening assay, may be cultured in media that supports regeneration of plants. In some embodiments, any suitable plant tissue culture media (e.g., MS and N6 media) may be modified by including further substances, such as growth regulators. Tissue may be maintained on a basic media with growth regulators until sufficient tissue is available to begin plant regeneration efforts, or following repeated rounds of manual selection, until the morphology of the tissue is suitable for regeneration (e.g., at least 2 weeks), then transferred to media conducive to shoot formation. Cultures are transferred periodically until sufficient shoot formation has occurred. Once shoots are formed, they are transferred to media conducive to root formation. Once sufficient roots are formed, plants can be transferred to soil for further growth and maturity.

[0094] The excision of an endogenous gene (e.g., BnIND, BnALC, or BnPGAZ) in regenerating plants can be confirmed by one or more assays, for example, a molecular biological assay, such as Southern blotting, Northern blotting, or PCR; a biochemical assay, such as detecting the absence of a protein product by immunoassay (ELISA or Western blot) or by screening for reduced enzymatic function; plant part assays, such as leaf or root assays; and analysis of the phenotype of the whole regenerated plant. In aspects of the disclosure, KASPAR.TM. and TAQMAN.TM. assays are provided to determine the zygosity of endogenous genes BnIND, BnALC, or BnPGAZ in the regenerating plants.

[0095] Using the methods disclosed herein, BnIND, BnALC, or BnPGAZ dropout variant plants are generated. For example, a dropout variant plant comprise excised endogenous genomic sequence of at least one allele of the BnIND-A gene (SEQ ID NO:105, 106, 107), BnIND-C gene (SEQ ID No:108), or combinations thereof; at least one allele of the BnALC-A gene (SEQ ID No:120), BnALC-C gene (SEQ ID No:121), or combinations thereof; or at least one allele of BnPGAZ-A gene (SEQ ID No:112), BnPGAZ-C gene (SEQ ID No:116), or combinations thereof. In a further aspect, the modified B. napus plant comprises one, two, three or four excised alleles of the BnIND gene; one, two, three or four excised alleles of the BnALC gene; or one, two, three or four excised alleles of the BnPGAZ gene. In a preferred aspect, the modified B. napus plant comprises three or four excised alleles of the BnIND gene; three or four excised alleles of the BnALC gene; or three or four excised alleles of the BnPGAZ gene.

V. Cultivation and Use of Transgenic Plants

[0096] A plant exhibiting a targeted genomic modification according to the present invention may have one or more desirable traits. Such traits can include, for example: resistance to insects and other pests and disease causing agents; tolerances to herbicides; enhanced stability, yield, or shelf-life; environmental tolerances; pharmaceutical production; industrial product production; and nutritional enhancements. The desirable traits may be the result of the excision of endogenous genomic sequence or gene through the introduction of a CRISPR-associated nuclease and guide RNAs that flank the endogenous genomic sequence or gene. The elimination of the endogenous genomic sequence or gene results in a desirable trait which can then be introgressed to other plants or inherited by subsequent generations of the plant.

[0097] Thus, in one aspect, the desired trait can be due to the excision of endogenous genomic sequence or gene in the plant. In an additional aspect, the desirable trait can be obtained through conventional breeding, which trait may be developed by a CRISPR-Cas9 based gene editing approach by creating gene dropout mutants for one or more endogenous genes. In an aspect, the desirable trait is increased pod shatter tolerance. In an additional aspect, the one or more endogenous genes are involved in fruit dehiscence and comprise BnIND, BnALC, or BnPGAZ or combinations thereof.

[0098] Plants exhibiting endogenous genomic sequence or gene excision according to the invention may be used or cultivated in any manner, wherein transmission of the excised nucleic acid sequence to other plants is undesirable. Accordingly, modified plants that have been engineered to, inter alia, have one or more desired traits, may be transformed with nucleic acid molecules according to the invention, and cropped and cultivated by any method known to those of skill in the art.

[0099] Several methods of mechanical testing of pod shatter are described in the art including the cantilever test (Kadkol et al. 1984. Euphytica, 33, 61-71) and the pendulum method (Liu et al. 1994. Journal of Texture Studies, 25, 179-189). Additionally, the Examples provided in this disclosure describe laboratory testing and field testing methods that can be used to identify and quantify pod shatter tolerance phenotype.

[0100] Disclosed herein is a method to evaluate pod shatter tolerance in a laboratory. This method for laboratory phenotyping pod shatter tolerance includes adding closed pods to a container, adding ball bearings to the container and shaking the container with a mechanical shaker.

[0101] Also provided herein is a method to measure shatter tolerance in the field that includes growing plants in a replicated field trial; introducing a shatter inducing treatment to the plants. Shatter treatment includes the application of wind at average speeds of from 100 km/h to 200 km/h, 100 km/h to 150 km/h, or 120 km/h to 140 km/h. In particular examples, wind speed can average approximately 100 km/h, 105 km/h, 110 km/h, 115 km/h, 120 km/h, 125 km/h, 130, km/h or 135 km/h. In other examples, wind speed can average approximately 140 km/h, 145 km/h, 150 km/h, 155 km/h, 160 km/h, 165 km/h, 170 km/h, 175km/h, 180, km/h 185 km/h, 190 km/h, 195 km/h, or 200 km/h. Applied wind can be generated using a blower mounted in front of a tractor; applying the treatment 12 times at a tractor speed of --5 km/h; varying the wind angle compared to planted rows from perpendicular to oblique; and allowing natural additional shatter pressure for an additional two weeks where pod integrity is challenged due to weather related events such as moisture, rain, dryness, temperature and natural wind.

[0102] Shatter tolerance can be assessed based on the condition of pods following treatment which can be half-shattered, fully shattered, or unshattered, as defined herein. For laboratory phenotyping, "percent shattered pods" or "SHTPC" is the number of fully shattered +half shattered pods/total number of pods*100. For field phenotyping, "half shattered" pods are not counted, so "percent shattered pods" or "SHTPC" is the number of fully shattered/total number of pods*100. SHTPC is compared to the wildtype after the shatter inducing treatment.

[0103] The following are examples of specific embodiments of some aspects of the invention. The examples are offered for illustrative purposes only and are not intended to limit the scope of the invention in any way.

EXAMPLES

Example 1: Constructs and Guide RNA Selection for Genome Editing of Arabidopsis IND and ALC

[0104] The first approach to improving shatter tolerance through gene editing relies on creating dropouts of INDEHISCENT (IND) and ALCATRAZ (ALC), two genes known to be involved in pod shatter. However, all Arabidopsis and Brassica mutants have either been generated by Ethyl Methane Sulfonate (EMS) mutagenesis and consist in frameshifts occurring in the genes coding sequences, or have been generated through single guide gene editing (Rajani and Sundaresan, 2001; Liljegren et al., 2004; Braatz et al., 2017; Braatz et al., 2018; Braatz et al., 2018). It is therefore unknown that complete deletion of the IND and ALC genes would result in improved pod shatter tolerance. To test this hypothesis in Arabidopsis, plants with a dropout of the AtIND genomic region were created.

[0105] Arabidopsis INDEHISCENT (AtIND) GenBank locus ID is AT4G00120 (SEQ ID NO:36). The AtIND gene is localized on Chr4: (40000 . . . 45000).

[0106] CRISPR-Plant and a suitable guide RNA identification program was used to identify guides that were in the 40000-41804 bp and 42400-45000 bp regions of chromosome 4. (Xie et al. 2014. Molecular Plant. 7(5): 923-6). Guide RNAs and position of primers are shown in Table 3 and FIG. 1.

TABLE-US-00003 TABLE 3 Guide RNA (first SEQ ID base changed to G NO: Guide name Pair# when something else) 37 AT-IND-CR8 3 GCCCATGGATAAATGTGCCT 38 AT-IND-CR6 2 GTCCAAACTGTCTACACCGA 39 AT-IND-CR2 1 GTGAAGCAGGGTCGACCTTT 40 AT-IND-CR3 1 GGGGAATCAGTATATTAGCT 41 AT-IND-CR5 2 GGATTGACTGTCTAATTGGG 42 AT-IND-CR7 3 GCTGATTAAATTGTTCATGC

[0107] Sequences for expressing gRNAs were built into three constructs with the following gRNA combinations: AT-IND-CR2-CR3, AT-IND-CR5-CR6, and AT-IND-CR7-CR8. The guide RNA sequences were subcloned into proprietary vectors and cloned using Invitrogen Gateway technology (Thermo Fisher Scientific, Waltham, MA) in a specially designed vector that contained a GM-GY1 PRO:DS-RED:UBQ3TERM cassette for seed sorting and a NOS PRO:BAR:NOSterm cassette for herbicide selection. Constructs were transformed in GV3101 Agrobacterium.

[0108] The same approach was followed for Arabidopsis ALCATRAZ (AtALC), corresponding to GenBank locus ID AT5G67110 (SEQ ID NO:43). The AtALC gene is localized at Chr5: 26780000: :26791000. Selected AtALC guide RNAs are shown in Table 4 and placement is shown in FIG. 2. Constructs were made for each of the following gRNA combinations: AT-ALC-CR1-CR2, AT-ALC-CR3-CR6, AT-ALC-CR5-CR4, AT-ALC-CR4-CR7, and AT-ALC-CR2-CR8. These guide RNAs were expressed from constructs to induce excisions of the genomic region containing AtALC and/or its promoter and thereby create AtALC dropouts. Because the ICU2 gene runs into the 3' UTR of AtALC, several of the guide RNAs (AT-ALC-CR1 through AT-ALC-CR6) clip the ICU2 gene. Consequently, sequences encoding guides AT-ALC-CR7-CR8 were designed to target the AtALC promoter chr5:26786433-26787809. The cloning strategy described herein to create AtIND constructs was also used to create AtALC constructs.

TABLE-US-00004 TABLE 4 Guide RNA sequence SEQ ID (first base changed to NO: G when required) Guide name Pair# 44 GTCTCTGTGCCCTGGCTCCC AT-ALC-CR1 1 45 GCCAGCCTACGCTGGTGGAC AT-ALC-CR3 2 46 GAGACCGGTGTGCTTATGGA AT-ALC-CR5 3 47 GCGTTCTTCAGAGAAGCGCG AT-ALC-CR6 2 48 GGACACCAGATTCTGTCTAT AT-ALC-CR2 1 49 GTCTTCAGAGAAGCGCGTGG AT-ALC-CR4 3 50 GTGAAATCCAACGAGATAAA AT-ALC-CR7 1 51 GAACTTGCCATCTAAATAAA AT-ALC-CR8 2

[0109] This example demonstrates the selection of guide RNAs targeting the AtIND and AtALC genes, the production of constructs for transformation incorporating the gRNA pairs.

Example 2: Selection of T1 Seeds and Germination Rates

[0110] Constructs for AtIND and AtALC dropouts described in Example 1 were transformed in Arabidopsis Columbia-0 using a floral dip protocol described in Clough and Bent, 1998, The plant journal, 16(6), 735-743. Seeds of primary transformants were harvested and the presence of transgenic seeds for each construct identified by visualizing under red fluorescence filter. On average, more red seeds were observed for the AtIND-related constructs than for the AtALC-related constructs. Some DsRed positive seeds of AtALC-related constructs appeared shriveled at the base in the chalazal region.

[0111] Approximately 100 DsRed positive seeds for IND93017 (AT-IND-CR2-CR3) and ALC93022 (AT-ALC-CR5-CR4) were selected and planted in 36-pot flats. Flats were left at 4.degree. C. for 3 days and plants were grown in a growth chamber using a diurnal program with 16 hours light, 23.degree. C., 55% relative humidity (RH), 8 hours dark, 20.degree. C., 55% RH.

[0112] Germination rate of IND93017 was 76.4%, while the germination rate of ALC93022 was significantly lower at 18.5%. In addition, 15 (out of 20 total) ALC93022 plants displayed a normal phenotype; and the other 5 plants showed a clearly abnormal phenotype of stunted plants and shriveled leaves. All IND93017 plants looked phenotypically like wild-types. The AtALC phenotype is likely due to the proximity and disruption of the ICU2 gene.

[0113] This example demonstrates the successful transformation of AtIND and AtALC dropout constructs and successful germination of resultant T1 seeds.

Example 3: Genotyping of IND93017 and ALC93022 T1 Plants

[0114] Genomic DNA of T1 plants was extracted using GENEJET Plant Genomic DNA purification kit (Thermo Fisher Scientific, Waltham, MA). PCR amplification of RNA guide pairs was carried out with 50 ng template DNA, 10 .mu.M each forward and reverse primers, and 2.times. PHUSION Master Mix (New England BioLabs Inc., Ipswich, Mass.) and a profile of 30 seconds initial denaturation at 98.degree. C., followed by 35 cycles of 98.degree. C. for 10 seconds, 60.degree. C. for 20 seconds and 72.degree. C. for 30 seconds, and ending with a final extension of 72.degree. C. for 5 minutes. For full locus amplification, the same PCR mix and amplification profile were used, except primer extension was at 72.degree. C. for 3 min instead of 30 seconds. Genotyping of the IND93017 T1 plants was performed using primers IND-3,5,7_F-Pair#1(SEQ ID NO:52) and IND-2,6,8_R-Pair#1 (SEQ ID NO:53) and results are shown in Table 5.

TABLE-US-00005 TABLE 5 Description Number % Plants with data 89 100 Plants with a detectable dropout 42 47.2 (candidate het and homo) Plants with candidate homozygous dropout 16 18.0 Plant with candidate het dropout 26 29.2 Plants with WT 47 52.8

[0115] ALC93022 T1 plants with normal phenotypes were genotyped as described above using ALC-1,2_F-Pair#1 (SEQ ID NO:54) and ALC-2,4,6_R-Pair#1 (SEQ ID NO:55) primers. None of the T1 plants with normal phenotypes had a detectable dropout when genotyped.

Example 4: Genotyping and Phenotyping of IND93017 AtIND Dropouts

[0116] The progeny of 3 candidate homozygous dropout T1 plants 1, 71, and 85 were planted and genotyped. All plants corresponding to variants 1 and 85 appeared to have inherited the dropout mutation. None of the WT plants have the dropout mutation. The progeny of variant 71 had both detected dropout and WT bands and was not carried over for phenotyping.

[0117] A total of 32 plants corresponding to the progeny of TO dropout variants 1 and 85 and a set of wildtype Col-0 plants were phenotyped using a GENO/GRINDER (SPEX.RTM. SamplePrep, Metuchen, N.J.) with 10 siliques per vial and two reps per plants. Initial shaking conditions were 750 rpm for 15 seconds, then 850 rpm for 30 seconds, and then used 1500 rpm for 30 seconds. The phenotype was recorded as fully shattered pods (both valves detached from the replum and seeds dispersed), half shattered (one valve detached from the replum and half seeds dispersed) or unshattered pods (both valves attached and containing seeds).

[0118] Increasing rpm and time conditions increased the shattering of wildtype plants reaching 100% at 1500 rpm for 30 seconds. All AtIND dropout variant 1 plants were shatter tolerant compared to wildtype plants with only 3 siliques shattered out of 240 tested (1.25%) at 1500 rpm for 30 seconds. The broken mutant siliques were broken in half horizontally with both valves still attached but seeds were fully out of the siliques. The progeny of dropout variant 85 appeared to still be segregating for the mutation. Eleven plants were shatter tolerant while 7 were shatter susceptible compared to wildtype plants.

[0119] This example shows that AtIND dropout variant plants having an indehiscent phenotype were successfully created in Brassicaceae species Arabidopsis thaliana.

Example 5: Constructs and Guide RNAs for Genome Editing of Brassica napus IND Via Microspore Bombardment

[0120] The gene editing dropout approach described above for Arabidopsis was adapted and evaluated to determine if it could improve shatter tolerance in B. napus. Constructs were designed to create dropouts of IND, ALC, and polygalacturonase expressed in abscission zone (POLYGALACTURONASE; PGAZ) in proprietary Brassica napus germplasm.

[0121] Gene edits were created in Brassica napus IND-A and IND-C genomes (BnIND-A, SEQ ID NO:56 and BnIND-C, SEQ ID NO:57) in B. napus line NS1822BC. Microspore bombardment of linearized plasmids was used. One linearized plasmid vector contained the selectable marker Glyphosate N-Acetyltransferase (GAT) gene variant 4621 for herbicide resistance, a separate vector was used to express Streptococcus pyrogenes Cas 9, and a third vector was used to deliver expression of BnIND target specific guide RNAs. Guide RNAs were built as a fusion of CRISPR RNA (crRNA) BNA-IND-CR1 (SEQ ID NO:26) and BNA-IND-CR2 (SEQ ID NO:27), each was fused to trans-activating CRISPR RNA (tracrRNA) of Streptococcus pyrogenes. BNA-IND-CR1 and BNA-IND-CR2 were designed to target both BnIND-A and BnIND-C genes in NS1822BC as shown in FIG. 3. Method of transforming B. napus microspores for genome editing are described in PCT/US2019/34531, filed May 30, 2019 which is incorporated herein by reference in its entirety.

[0122] After microspore bombardment and regeneration, transgenic plants were screened for the presence of genomic dropouts through sequencing PCR amplicons. Forward primer IND_CR1-FP, TTGTCAAAAAGAGGAGAGAGAGG (SEQ ID NO:142) and reverse primer IND_CR1-RP, GAAAGATCAAGAACACAGGA (SEQ ID NO:143) flanking the IND-CR1 cut site and forward primer IND CR2-FP, CGACCAAAAATATGAAATACT (SEQ ID NO:144) and reverse primer IND_CR2-RP, GCTACGGCTAACACACATACG (SEQ ID NO:145) flanking the IND-CR2 site as shown in FIG. 3 were designed for amplification. Both site specific assays and a spanning assay (IND-CR1-FP/IND-CR2-RP) were used. Amplicon reads were differentially assigned to the A and C genomes because of single nucleotide differences in the BnIND-A and BnIND-C predicted amplicon sequences.

[0123] Transgenic plants with detected dropouts were backcrossed to wildtype NS1822BC plants. T1 plants were screened for the presence of dropouts (2,607 bp deletion for BnIND-A and 2,426 bp deletion for BnIND-C) and plants showing a deletion were selfed to create homozygous dropouts. T2 plants homozygous for BnIND-A or BnIND-C dropouts without plasmid components were identified using Southern by Sequencing (SbS) and selfed to create T3 homozygous seeds. One NS1822BC BnIND-A dropout variant (SEQ ID NO:58) and two NS1822BC BnIND-C dropout variants (SEQ ID NO:59) were created.

[0124] This example describes the construction of guide RNAs and plasmid vectors used to produce BnIND-A and BnIND-C dropout plants via microparticle bombardment; and their confirmation by molecular analysis of targeted dropouts in B. napus T1 and T2 plants.

Example 6: Development of TAQMAN.TM. Molecular Assays for NS1822BC IND-A and IND-C Dropouts

[0125] TAQMAN.TM. assays were developed for use as molecular markers to track the BnIND-A and BnIND-C1 and BnIND-C4 (BnIND-C1 and BnIND-C4 are the same dropout obtain from different events). TAQMAN.TM. markers can be used to track individual dropouts and zygosity. TAQMAN.TM. primer and probe for each loci (BnIND-A and BnIND-C) are shown in Table 6.

TABLE-US-00006 TABLE 6 SEQ ID NO: Oligo Name Sequence 81 NS1822BC_C75 IND-C FAMProbe TCTCTTTCCTTCTTCATC 82 NS1822BC_C75 IND-C VICProbe CTTGGTTCTAGAAGCATAA 83 NS1822BC_C75 IND-C FP AAAATGGAGACATGTGTTGAGTAACTA 84 NS1822BC_C75 IND-C RP TTTGGACTAATGCCCAACTGA 85 NS1822BC_C75 IND-C FP2 TGATATCATCACCAAAGTGCAA 86 NS1822BC_C75 IND-C RP2 CAAAATCATGGTTACTCAAAAAGC 87 NS1822BC_C7B IND-C FAMProbe TCTCTTTCCTTCTTCATC 88 NS1822BC_C7B IND-C VICProbe CTTGGTTCTAGAAGCATAA 89 NS1822BC_C7B IND-C FP AAAATGGAGACATGTGTTGAGTAACTA 90 NS1822BC_C7B IND-C RP TGGACTAATGCCCAACTGATT 91 NS1822BC_C7B IND-C FP2 TGATATCATCACCAAAGTGCAA 92 NS1822BC_C7B IND-C RP2 CAAAATCATGGTTACTCAAAAAGC 93 NS1822BC_C7M IND-A FAMProbe CTTATTCCAGACATTTG 94 NS1822BC_C7M IND-A VICProbe CTTGGTTCAGAAGCA 95 NS1822BC_C7M IND-A FP AAAATGGAGACATGTGTTGAGTAACTA 96 NS1822BC_C7M IND-A RP CTTCATTGTTTCATTGTTTCAGTAGC 97 NS1822BC_C7M IND-A FP2 TGGATATAACATATGAATCGGATAAGTG 98 NS1822BC_C7M IND-A RP2 TTAGAGAAAGGCTTGGTTCTCC 99 NS1822BC_C7N IND-A FAMProbe CTTATTCCAGACATTTG 100 NS1822BC_C7N IND-A VICProbe CTTGGTTCAGAAGCA 101 NS1822BC_C7N IND-A FP AAAATGGAGACATGTGTTGAGTAACTA 102 NS1822BC_C7N IND-A RP CTTCATTGTTTCATTGTTTCAGTAGC 103 NS1822BC_C7N IND-A FP2 GGATATAACATATGAATCGGATAAGTGT 104 NS1822BC_C7N IND-A RP2 TTAGAGAAAGGCTTGGTTCTCC

Example 7: Genome Editing of Brassica napus IND via Agrobacterium Transformation

[0126] BnIND-C dropout variants were created in B. napus lines G00010BC and G00555MC using an Agrobacterium transformation method based on Moloney et al. (1989) Plant Cell Reports 8: 238-242. Lines were transformed with a vector for expression of tracrRNAs containing BNA-IND-CR1 (SEQ ID NO:26) and BNA-IND-CR2 (SEQ ID NO:27) crRNAs. The presence and nature of the deletion as well as absence of transformation vector components was determined using SbS. No BnIND-A dropouts were identified in G00010BC because, as later discovered through whole genome sequencing, a region of --240-300kb is missing from chromosome 3A in this inbred. The map of the G00010BC BnIND-C locus and G00555MC BnIND-A and BnIND-C loci are shown in FIG. 4 and FIG. 5, respectively. Sequences of wildtype G00010BC BnIND-C (SEQ ID NO:67), wildtype G00555MC BnIND-A (SEQ ID NO:69), and wildtype BnIND-C (SEQ ID NO:70) are provided.

[0127] TO transformants were characterized at the molecular level for the presence of BnIND-A orBnIND-C dropouts using NextGen sequencing of PCR amplicons generated from genomic DNA of candidate dropout plants using primers having sequence provided in SEQ ID NOs:75, 76, 77, and 78, respectively. A G00010BC IND-C dropout (SEQ ID NO:68) was identified. The progeny of selected plants was grown for further analysis. A T1 transgene positive G00555MC plants with a heritable dropout was identified. The plant was found to be heterozygous for a BnIND-A dropout (SEQ ID NO:75) and wildtype for the BnIND-C locus. The progeny of this plant was used to segregate the transgene and obtain BnIND-A G00555MC transgene free homozygous dropout and wildtype segregant plants.

[0128] The transgene that was still active in the T1 G00555MC plant heterozygous for a BnIND-A dropout and wildtype for the BnIND-C locus, still expressed guide RNAs and Cas9 in the germline and provided a possibility to generate and detect an BnIND-C dropout event in further progeny. Two plants (of 512 total G00555MC T2 plants analyzed) were found to be positive for an BnIND-C dropout (SEQ ID NO:76) and wildtype for the BnIND-A locus.

[0129] These plants were backcrossed to wildtype parental lines in order to segregate T-DNA from the dropout allele. They were also crossed to transgene-free G00555MC plants homozygous dropouts for the BnIND-A to create transgene free homozygous double BnIND-A/C dropout plants, which can be used as males to create hybrids.

[0130] This example demonstrates the production of BnIND-A/C dropout plants, which were confirmed through molecular characterization.

Example 8: Discovery and Validation of a Natural Deletion Harboring INDEHISCENT Gene (IND-A) in G00010BC

[0131] An investigation of the inability to generate BnIND-A dropouts in G00010BC revealed a natural deletion in the INDEHISCENT gene on chromosome 3A (IndA).

[0132] Whole genome sequence was performed and the two main homoeologous copies of INDEHISCENT genes from chromosomes N03 (BnIND-A; BnaA03g27180D) and N13 (BnIND-C; BnaC03g32180D) were compared to five references genomes using the Basic Local Alignment Search Tool (BLAST.TM.) algorithm available from the National Center for Biotechnology Information (NCBI). Altschul et al. (1990) J. Mol. Biol. 215: 403-10). The five reference genomes were DH12075 (public spring canola), Darmor (public winter canola), and three high quality third generation proprietary spring canola lines (NS1822BC, G00010BC, and G00055MC). Orthologous matches for both genes were found in all genomes, except for BnIND-A in G00010BC. Comparative global sequence alignment of an extended genomic region surrounding BnIND-A revealed a large segmental deletion in the G00010BC genome. The deletion is from 229 -- 307 kb in length, depending on reference genome used for alignment. The physical starting and ending position of the BnIND-A deletion segment, as determined by alignment to each reference genome, is shown in Table 7.

TABLE-US-00007 TABLE 7 Genome Start Position End Position Length (bp) NS1822BC 14,710,075 14,958,191 248,116 DH12075_v1.1 14,449,859 14,690,232 240,373 Darmor_v4.1.1 13,306,888 13,535,922 229,034 G0055MC 14,915,907 15,223,431 307,524 G00010BC 14,989,780 14,989,781 (deletion breakpoint)

Example 9: Molecular Assays to Detect the BnIND-A Natural Deletion in G00010BC

[0133] The G00010BC and wildtype reference N3 sequences were used to develop molecular assays to detect the presence or absence of native BnIND-A deleted segment as well as sequences flanking the breakpoint site of the deletion.

[0134] KASPAR.TM. assay comprised of four primers was developed using an assay design algorithm available as Kraken.TM. (LGC Genomics, Hoddesdon, Hertfordshire, UK). Initially, BnIND-A gene sequence on N03 was compared to the homoeologous BnIND-C gene on N13 to identify unique polymorphisms. Potential primer sequence targets were then identified to detect the wildtype and natural deletion states of BnIND-A. To detect the natural deletion, allele-specific, fluorescently tagged forward primer and common reverse primers flanking the breakpoint were designed as shown in FIG. 6 (N03 Deletion). To detect the wildtype state, a different fluorescently tagged forward primer and reverse primer were designed to hybridize within the native deletion segment sequence as shown in FIG. 6 (N03 WT). The primer sequences shown in Table 8 were used in a four primer, co-dominant composite marker KASPAR.TM. assay to detect the presence or absence of BnIND-A deletion segment.

TABLE-US-00008 TABLE 8 SEQ ID NO: Oligo Name Description 131 IND_A_001_F001 WT Forward Primer 132 IND_A_001_R001 WT Reverse Primer 133 BP_F003 Mutant Forward Primer 134 BP_R003 Mutant Reverse Primer

[0135] The KASPAR.TM. assay mixture was composed of 12 .mu.l of 100 mM of each forward primer and 30 .mu.l of 100 mM of each reverse primer. 13.6 .mu.l of this mixture was combined with 1000 .mu.l of KASP Master Mix.TM. (LGC Genomics, Hoddesdon, Hertfordshire, UK). A Meridian.TM. (LGC Genomics) liquid handler dispensed 1.3 .mu.l of the mix onto a 1536 plate containing .about.6 ng of dried DNA. The plate was sealed with a Phusion.TM. laser sealer (LGC Genomics) and thermocycled using a Hydrocycler.TM. (LGC Genomics) under the following conditions: 95.degree. C. for 15 minutes (min), 10 cycles of 95.degree. C. for 20 seconds (sec), 61.degree. C. stepped down to 55.degree. C. for 1 min, 29 cycles of 95.degree. C. for 20 sec, and 55.degree. C. for 1 min. The excitation at wavelengths 485 (FAM) and 520 (VIC) was measured with a Pherastar.TM. plate reader (BMG Labtech, Offenburg, Germany). Values were normalized against the passive reference dye ROX (i.e., 5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester), plotted and scored on scatterplots utilizing the Kraken.TM. software (LGC Genomics).

[0136] A variation on conventional TAQMAN.TM. end-point genotyping system was developed. Conventional TAQMAN.TM. assays use a forward and reverse primer and two fluorescent labeled probes. The TAQMAN.TM. variation developed to detect the presence or absence of the BnIND-A deletion segment is a compound assay that comprises two independent amplification reactions. The first reaction amplifies and detects wildtype gene sequence using forward and reverse primers capable of hybridizing to sequences that flank the 3' breakpoint of the BnIND-A deletion segment in wildtype N03 chromosome as shown in FIG. 7 (N03 WT). Because the deletion segment is present in wild type N03 chromosome, the wildtype primers amplify sequence upstream the 3' breakpoint and the amplified sequence is detected using a wild type probe. The second assay reaction detects the presence of BnIND-A deletion using a deletion forward primer that hybridizes upstream of the 5' breakpoint and a deletion reverse primer that hybridizes downstream of the 3' breakpoint of the deletion as shown in lower portion of FIG. 7 (N03 Deletion). When the deletion segment is missing, the second assay amplifies sequence containing both the joined 5' breakpoint and 3' breakpoint. By contrast the second assay will not amplify N03 genomic sequence that includes the deletion segment because the 5' and 3' breakpoints are too far apart to be amplified effectively under assay conditions. The primers and probes for both wildtype and deletion reactions (4 primers and two probes shown in Table 9) were combined and the reactions were run simultaneously.

TABLE-US-00009 TABLE 9 SEQ ID NO: Oligo Name Description 135 N101T11-F001 WT and mutant common F Primer 136 N101T11-R001 WT R Primer 137 N101T11-001-X001 WT Probe 138 N101T10-R001 Deletion R Primer 139 N101T10-001-X001 Deletion Probe

[0137] The combination TAQMAN.TM. assay included 13.6 .mu.l of a primer probe mixture (18 .mu.M of each probe, 4 .mu.M of each primer) and 1000 .mu.l of master mix from ToughMix.TM. kit (Quanta Beverly, Mass.). A liquid handler dispensed 1.3 .mu.l of the mix onto a 1536 plate containing .about.6 ng of dried DNA. The plate was sealed with a laser sealer and thermocycled in a Hydrocycler device (LGC Genomic Limited, Middlesex, United Kingdom) under the following conditions: 94.degree. C. for 15 min, 40 cycles of 94.degree. C. for 30 secs, 60.degree. C. for 1 min. PCR products are measured using at wavelengths 485 (FAM) and 520 (VIC) by a Pherastar.TM. plate reader (BMG Labtech, Offenburg, Germany). The values are normalized against ROX and plotted and scored on scatterplots utilizing the Kraken.TM. software. The combined TAQMAN.TM. produced the results of co-dominant assay and was capable of distinguishing and displaying sample clusters that were homozygous wildtype for BnIND-A, homozygous BnIND-A deletions, and hemizygous deletions (WT/of BnIND-A deletion)

[0138] This example describes the development of diagnostic assays for the detection of and introgression of the BnIND-A natural deletion disclosed herein.

Example 10: Description and Validation of Lab Phenotyping For Pod Shatter Tolerance

[0139] A laboratory assay was developed to evaluate the shatter resistance of pods subjected to mechanical agitation at specified speeds and times. GENO/GRINDER device (SPEX.RTM.SamplePrep, Metuchen, N.J.) was used to mechanically break canola pods and thereby assess shattering tolerance or susceptibility. Different speeds (rpm) and times were tested with intact pods from inbred NS1822BC grown in controlled environment growth chamber (Conviron, Winnipeg, Canada). Fully shattered pods (both valves detached from the replum and seeds dispersed), half shattered (one valve detached from the replum and half seeds dispersed) or unshattered pods (both valves attached and containing seeds) were measured. Fifteen pods were used for each data point. Results showed a linear relationship between the mechanical speed at which pods are agitated and the number of shattered (full+half shattered) pods (r2=0.944).

[0140] In another experiment, a group of 15 pods (ranging from 3 to 6 cm in length) for three different inbred lines, NS1822BC, G00010BC, and G00055MC grown in a controlled environment were tested. Results indicated a direct relationship between speed of mechanical agitation and pod shatter for the inbreds that were tested. Pods of all three inbreds were almost fully shattered at speed equal to or greater than 1200 rpm.

[0141] At 750 rpm, pod shattering of G00010BC was found to be significantly lower than the other two inbreds. These results show that, compared to the other two inbreds evaluated under the foregoing conditions, the native BnIND-A deletion line (G00010BC) provided an improved shatter tolerance phenotype.

[0142] A second laboratory assay phenotyping experiment was conducted using 5 to 6 plants of each the foregoing three genotypes. As described above, plants were grown in a growth chamber, and pods collected at maturity were phenotyped using the GENO/GRINDER assay (15 pods at 1000rpm for 30 sec). Percentage shattered pods was recorded for each assay repetition. The results shown in FIG. 8 demonstrated statistically significant differences (t-test: two-sample assuming unequal variances, p<0.01) between indicated genotypes. Pods collected from G00010BC were found to be on average significantly more shatter tolerant than pods collected from the other two genotypes. These results provide additional evidence that the presence of the IND-A natural deletion in G00010BC contributes to higher mechanical resistance and an improved shatter tolerance phenotype as compared to the other two genotypes tested.

[0143] This example describes a laboratory assay to induce pod shattering and evaluate pod shatter tolerance. Results of two studies using the assay showed increased shatter tolerance for G00010BC relative to lines NS1822BC and G00055MC and provided evidence that the natural BnIND-A deletion in G00010BC contributes to increased shatter tolerance.

Example 11: Laboratory Phenotyping of T2 BnIND-C Dropout and Wildtype Plants

[0144] Second generation T2 G00010BC BnIND-C homozygous dropout variant and wildtype plants were grown in controlled environment growth chambers (Conviron) under standard conditions. Pods were harvested at maturity after 2 weeks without water. Pods were left to acclimate in the laboratory at 23 .degree. C. for 5 days. Fifteen pods of similar sizes were harvested for 5 individual G00010BC homozygous dropout plants and 5 segregating wild-type plants. Pods from individual plants were placed in plastic boxes of 12.times.8.5.times.6.5 cm and mechanically agitated at 1700 rpm for 30 seconds using GENO/GRINDER device. After disruption, individual pods were scored according to half shattered, fully shattered, and unshattered phenotype. Total number of shattered pods was calculated as the sum of the half shattered and fully shattered pods. The average percentage of shattered pods for homozygous BnIND-C dropout variants was 0.00%, as compared to an average of 92.00% shattered pods for wildtype plants. Thus, non-functional gene deletion of the IND gene in the C genome produced a fully controlled genetic trait that can impart shatter tolerance in B. napus.

[0145] This example demonstrates the successful development of homozygous BnIND-C dropout variant in Brassica napus that show an indehiscent phenotype.

Example 12: Laboratory Phenotyping of T3 Heterozygous and Homozygous IND-C Dropout Plants

[0146] Sixty-four T3 seeds from two T2 G00010BC plants heterozygous for the BnIND-C dropout were planted and genotyped using a dropout specific PCR assay followed by NextGen sequencing (as described in Example 5). Plants that were homozygous (9), heterozygous (10), and wildtype (8) for the BnIND-C dropout were identified and grown to maturity in a growth chamber in 16 hour light (23.degree. C.) (.about.360 .mu.E light intensity) and 8 hour dark (20.degree. C.) regimen at .about.55% humidity. At maturity, plants were allowed to dry. Pods were harvested and phenotyped using the GENO/GRINDER laboratory assay at 1100 rpm for 15 seconds. For each replication using 15 intact pods, pods were visually inspected after the assay and classified according to fully shattered, half shattered or unshattered pod phenotype.

[0147] FIG. 9 shows shatter tolerance of G00010BC plants segregating for a BnIND-C dropout calculated as the average percentage of shattered pods from replicated assays. The number of knocked out alleles (KO) are indicated for each zygosity category, where 4 KO are homozygous for the BnIND-C dropout, 3 KO are heterozygous for the BnIND-C dropout, and 2 KO do not include any BnIND-C dropout alleles. Since G00010BC background has a native --300kb deletion on chromosome 3 including BnIND-A and therefore already 2 deleted alleles (or 2 knockouts and thus 2 KO). Plants heterozygous for the BnIND-C dropout have three deleted alleles (or 3 knockouts and thus 3 KO) and homozygous plants have four missing alleles (or 4 knockouts and thus 4 KO). B napus plants with 4 KO showed a strong shatter tolerant phenotype, and plants with 3 KO showed a significant reduction in the percentage of shattered pods compared to unmodified G00010BC plants (t-test, p<0.05).

[0148] This example shows that number of knocked out IND alleles correlated with pod shatter tolerance. Double knockout plants (all A and C alleles knocked out) showed higher shatter tolerance than plants with 3 knocked out alleles (2 KO for A and 1 KO for C).

Example 13: Description and Validation of Field Phenotyping For Pod Shatter Tolerance

[0149] Plants were grown in a replicated trial in Rockwood, Ontario, Canada. Plants in the field received a shatter-inducing treatment in the form of 135 km/h wind generated by a blower mounted in front of a tractor. The treatment was applied 12 times at a tractor speed of .about.5 km/h four months after planting. Wind angle compared to planted rows was varied from perpendicular to oblique for maximum effect. The trial saw additional shatter pressure for another two weeks after this shatter inducing treatment due to weather related events such as moisture, rain, dryness, temperature and natural wind. Percent shattered pods (SHTPC) was determined using visual evaluation of plants from 5 replications. Intensity of shatter pressure was evaluated using the following reference lines: 45H33 is a moderately susceptible check line; 45CM39 and 45M35 were used as shatter resistant checks. Results showed statistically significant separation between SHTPC of the most shatter susceptible hybrid 45H33 (60% shattered pods), and shatter tolerant HARVESTMAX hybrids 45M35 and 45CM39 (.about.30% shattered pods). A statistically significant difference was also found between susceptible (wildtype G00555MC and G00182MC) and tolerant (N00644BC and NS7627MC) inbreds, both indicative of significant shatter pressure on the trial.

[0150] The foregoing provides a validated field method for phenotyping pod shatter under controlled conditions that induce pod shatter. The method successfully distinguished between shatter susceptible and shatter tolerant lines of B. napus plants.

Example 14: Lab Phenotyping of BnIND-C Dropout and Wildtype Plants Grown in the Field

[0151] Pods were collected at maturity from multiple plants grown in one of the six field replications described in Example 6 prior to the field shatter inducing treatment and phenotyped in the laboratory using the GENO/GRINDER assay.

[0152] Intact pods of similar sizes were collected from untransformed NS1822BC wildtype plants and gene edited plants having an BnIND-A and/or BnIND-C dropout at different zygosity levels. Percentage shattered pods after GENO/GRINDER assay (1000rpm, 15 sec.) are shown in FIG. 10.

[0153] NS1822BC plants with two dropout alleles (homozygous BnIND-A, homozygous BnIND-C1 or double heterozygous BnIND-A and BnIND-C1) showed statistically significant increases in shatter tolerance compared to wildtype plants. In this assay, plants with only one dropout allele (heterozygous BnIND-A or heterozygous BnIND-C1) did not show significant difference in pod mechanical resistance compared to wildtype untransformed control plants.

[0154] These results indicate that deletions of either BnIND-A (as shown by the natural BnIND-A deletion found in G00010BC) or BnIND-C can significantly contribute to the shatter tolerant phenotype of an B. napus plant (in this case inbred line NS1822BC).

[0155] Intact pods of similar sizes were collected from G00010BC plants having the native BnIND-A deletion disclosed herein and gene edited G00010BC plants that were homozygous or heterozygous for a BnIND-C dropout. The mechanical resistance of these pods was compared to that of a commercially released pod shatter tolerant (PST) line check, which is referenced herein as PST Check 1. Pods were shaken in the GENO/GRINDER at 1500 rpm for 15 sec and results are presented in FIG. 11. At this speed, only about 1% of PST Check 1 and G00010BC BnIND-C homozygous (4 KO) dropout pods shattered. Pods of G00010BC BnIND-C heterozygous plants (3K0) produced approximately 4% fewer shattered pods than wildtype controls, though this difference was not statistically significant (t-test, p<0.01) due to the GENO/GRINDER assay use of significantly higher forces than forces used in the field phenotyping assay described in Example 13. That is, because higher forces overcome the mechanical resistance in more pods of both G00010BC plants (2K0) and G00010BC plants with a heterozygous BnIND-C dropout (3K0), the GENO/GRINDER assay did not detect the significant difference in pod shatter tolerance that was observed with this material in the field phenotyping study (FIG. 13) or the lab assay study described in FIG. 9.

[0156] This example demonstrates that combining BnIND-A deletion with 2 dropout alleles improved pod shatter tolerance induced under high forces of the laboratory assay conditions. Mechanical resistance was substantially improved relative to a combination of the BnIND-A deletion with a single other dropout allele.

Example 15: Field Phenotyping of BnIND Dropout and Wildtype Plants

[0157] Plants in five of the six field replications described in Example 13 were subjected to pod shatter inducing treatment. Plant pods were scored in the field.

[0158] NS1822BC inbred plants having different number of BnIND dropout alleles were grown in the same field and scored for pod shatter phenotype. Results in FIG. 12 show that plants harboring one or two dropouts of BnIND-A and/or BnIND-C performed statistically better in the field than the wildtype control. Plants homozygous for a BnIND-A dropout (IND-A Hom) or a BnIND-C dropout (IND-C Hom) showed a statistically significant .about.65% reduction (t-test, p<0.01) in SHTPC scores compared to wildtype plants. Plants heterozygous for the BnIND-C or the BnIND-A dropout presented statistically significant reductions in SHTPC scores compared to wildtype of .about.60% and .about.40%, respectively. Double heterozygous BnIND-A and BnIND-C dropout plants (IND-A/C Het) also demonstrated significantly increased shatter tolerance.

[0159] Wildtype and inbred G00010BC plants with different BnIND-C gene-edited dropouts were grown in the same field and characterized. G00010BC plants homozygous for BnIND-A native deletion and heterozygous BnIND-C dropout have three loss of function IND alleles (3 KO). These 3KO plants had significantly reduced SHTPC scores compared to wildtype and G00010BC (2KO) following shatter inducing treatment in the field. SHTPC scores of heterozygous plants were reduced by 40% to 45% compared to wildtype controls (WT and WT untransformed) (FIG. 13). Plants homozygous for the BnIND-C dropout did not shatter confirming the strong shatter tolerance of BnIND-A and BnIND-C double homozygous knockout.

[0160] This example confirmed lab assay results and showed that deletions of BnIND-A and BnIND-C contributed to increased field-induced shatter tolerance in different B. napus lines.

Example 16: Laboratory Phenotyping of Hybrid Plants Having Different Allelic Combinations of BnIND Dropouts

[0161] G00555MC SbS green plants homozygous for the BnIND-A dropout were crossed to G00010BC plants homozygous for an BnIND-C dropout and wildtype G00010BC plants to create hybrid plants with different allelic combinations of dropout and wildtype alleles. A small amount of hybrid seed was generated to grow plants for laboratory assay pod phenotyping. Specific allelic combinations of BnIND dropout variants are shown in Table 10 (for hybrid genotypes: first letter designates the male parent allele, second letter the female parent allele; upper case letter designates wild-type allele, lower case indicates a gene-edited dropout allele, except for bold and underlined "a" which designates G00010BC BnIND-A natural deletion allele).

TABLE-US-00010 TABLE 10 G00555MC male parent G00010BC female parent Hybrid genotype IND-A HO DO (aa/CC) IND-C HO DO (aa/cc) aa/Cc IND-A HO DO (aa/CC) IND-C WT (aa/CC) aa/CC IND-A WT (AA/CC) IND-C HO DO (aa/cc) Aa/Cc IND-A WT (AA/CC) IND-C WT (aa/CC) Aa/CC

[0162] G00555MC.times.G00010BC hybrid plants and hybrid checks were grown in a growth chamber. 45H33 was used as a moderately susceptible hybrid for pod shatter check. Shatter resistant checks were HARVESTMAX Hybrids 45CM39 and 45M35. Four plants were grown for each entry except for 45CM39 for which only 3 plants were grown. Intact pods were collected from individual plants and GENO/GRINDER assays were conducted using 10- At 750 rpm, pod shattering of G00010BC was found 15 pods per assays as described in Example 11. Percentages of shattered pods were calculated. Pods from each hybrid ranged from 3 cm to 6 cm in size and hybrids showed comparable pod size distributions except for 45H33 which had a higher number of smaller pods on average. Results of the phenotyping experiment are presented in FIG. 14.

[0163] HARVESTMAX hybrids (45CM39 and 45M35) showed a .about.50% reduction in percent shattered pods compared to 45H33 in this assay. This is similar to the difference in SHPTPC observed in the field for these hybrids. Accordingly, these results indicate that the laboratory assay results are sufficiently predictive to identify and distinguish shatter tolerant plants (HARVESTMAX hybrids) from moderately shatter susceptible plants (45H33) in a manner that is consistent with their shatter tolerance performance in the field. Compare FIG. 9 and FIG. 13 for G00010BC and FIG. 10 and FIG. 12 for NS1822BC.

[0164] The results of this assay also showed that a G00055MC x G00010BC hybrid with three (3) dropout alleles in aa/Cc allelic combination produced shatter resistance comparable to the commercial HARVESTMAX hybrids, which have been bred for improved shatter tolerance using existing natural variation. This allelic combination introduced into a HARVESTMAX genetic background further contributes to shatter tolerance of these hybrids.

[0165] In order to study the contributions of unique BnIND-A and BnIND-C functional alleles in a G00555MC x G00010BC hybrid, seeds for new allelic combinations were created using crosses between G00555MC BnIND-C homozygous dropout plants and G00010BC BnIND-C homozygous dropout plants and controls (Table 11). For hybrid genotypes: first letter designates the male parent allele, second letter the female parent allele; upper case letter designates wild-type allele, lower case indicates a gene-edited dropout allele, except for bold and underlined "a" which designates G00010BC BnIND-A natural deletion allele.

[0166] The shatter tolerance of these new allelic combinations (including Aa/cc) are tested in comparison to allelic combinations tested in FIGS. 14 and 45H33 and 45M35.

TABLE-US-00011 TABLE 11 G00555MC male parent G00010BC female parent Hybrid genotype IND-C HO DO (AA/cc) IND-C HO DO (aa/cc) Aa/cc IND-C HO DO (AA/cc) WT (aa/CC) Aa/cC

[0167] The foregoing demonstrates that B. napus hybrid plants having 3 BnIND dropout alleles produced average percent shattered pods scores comparable to commercial HARVESTMAX shatter tolerant hybrids checks and that introgression of the natural BnIND-A deletion of G00010BC, in combination with the CRISPR generated dropout alleles can generate hybrids having an improved shatter tolerance phenotype that is comparable to commercial shatter tolerant hybrids.

Example 17: Constructs and Guide RNA Selection for Genome Editing of B. napus ALC in G00010BC and G00555MC

[0168] The following guides were designed for use in genome editing excision (dropout) of the genes or the promoters of Brassica napus ALCATRAZ (BnALC) in the A and C genomes of Brassica lines G00010BC and G00555MC (Table 12). Vector construction and Agrobacterium transformation was done as described herein.

TABLE-US-00012 TABLE 12 SEQ ID NO. Guide name RNA Guide Sequence Dropout target 31 BNA-ALC-CR10 GAACGTAAGCCACCAGATTA BnALC-A gene 32 BNA-ALC-CR11 GTGGAGAGGAAATCTGACTT 33 BNA-ALC-CR12 GGAAGAATTCACAGAGAGAG BnALC-C gene 34 BNA-ALC-CR15 GGACTGAACAAAACTGCTAC 35 BNA-ALC-CR14 GCAACTAACGAAGAGACAGA BnALC-C promoter combined with CR12

[0169] ALC-CR10 is specific to BnALC-A (last G of PAM is missing in BnALC-C). ALC-CR11 is specific to BnALC-A (last G of PAM is missing in BnALC-C). ALC-CR12 is specific to BnALC-C (last G of PAM is missing in ALC-A). ALC-CR15 specific to BnALC-C (this sequence doesn't exist in BnALC-A). ALC-CR14 and ALC-CR12 will specifically delete --3kb of BnALC-C promoter as shown in FIG. 15. Genomic sequences for G00010BC BnALC-A (SEQ ID NO:71) and BnALC-C (SEQ ID NO:72) and G00555MC BnALC-A (SEQ ID NO:73) and BnALC-C (SEQ ID NO:74) are provided.

[0170] BnALC-A/C homozygous and heterozygous dropout plants provide improved pod shatter tolerance.

Example 18: Constructs and Guide RNA Selection for Genome Editing of Brassica napus PGAZ Gene by Microspore Transformation

[0171] Microspore bombardment of NS1822BC was also performed with linearized plasmids to deliver expression of Streptococcus pyrogenes Cas9, and another plasmid was used to deliver B. napus POLYGALACTURONASE (BnPGAZ) target specific guide RNAs, which were constructed as fusions of CRISPR RNA (crRNA) to BNA-PGAZ-CR1 and BNA-PGAZ-CR2, respectively, each of which was fused to trans-activating CRISPR RNA (tracrRNA) of Streptococcus pyrogenes. BNA-PGAZ-CR1 (SEQ ID NO: 28) and BNA-PGAZ-CR2 (SEQ ID NO:29) target both BnPGAZ-A (SEQ ID NO:60) and BnPGAZ-C (SEQ ID NO:61) genes in NS1822BC as shown in FIG. 16.

[0172] Amplicon NextGen DNA sequencing was used to identify four plants that carry a dropout mutation for BnPGAZ-A (SEQ ID NOs:61-62) and five plants that carry a dropout mutation for BnPGAZ-C (SEQ ID NOs:63-65). BnPGAZ-A dropout variants were crossed to BnPGAZ-C dropout variants to create plants containing different allelic combinations of dropouts in both genes. Microspores of heterozygous double dropout plants were used to create homozygous double dropout plants through microspore doubling and screening of regenerated plants.

[0173] The foregoing describes the use of guide RNAs and constructs to produce BnPGAZ-A and BnPGAZ-C dropout allele combinations in B. napus cells and plants by microspore bombardment, microspore doubling and crossing.

Example 19: Constructs and Guide RNA Selection For Genome Editing of Brassica napus PGAZ Gene by Agrobacterium Transformation

[0174] BnPGAZ-A and BnPGAZ-C TO dropouts were obtained in G00010BC and G00555MC using Agrobacterium transformation with plasmid PHP92921 and crRNAs BNA-PGAZ-CR11 (SEQ ID NO:30) and BNA-PGAZ-CR2 (SEQ ID NO:29). Plants were genotyped. Amplicon NextGen sequencing was used to identify dropouts for BnPGAZ-A (SEQ ID NO:77) and BnPGAZ-C (SEQ ID NO:78) in G00010BC, as well as dropouts for PGAZ-A (SEQ ID NO:79), and BnPGAZ-C (SEQ ID NO:80) in G00555MC.

[0175] The foregoing describes the use of guide RNAs and constructs to produce BnPGAZ-A and BnPGAZ-C dropouts in B. napus cells and plants.

[0176] All patent applications, patents, and printed publications cited herein are incorporated herein by reference in the entireties, except for any definitions, subject matter disclaimers or disavowals and except to the extent that the incorporated material is inconsistent with the express disclosure herein, in which case the language in this disclosure controls.

Sequence CWU 1

1

1451182PRTBrassica napus 1Met Ser Gly Ser Lys Ala Asp Ala Ala Ile Ala Pro Ile Val Met Met1 5 10 15Glu His His His Leu Leu Met Asn Trp Asn Lys Pro Ile Asp Leu Ile 20 25 30Thr Glu Glu Asn Ser Phe Asn His Asn Pro His Phe Ile Val Asp Pro 35 40 45Pro Ser Glu Thr Leu Ser His Phe Gln Pro Pro Pro Thr Ile Phe Ser 50 55 60Asp His Gly Gly Gly Glu Glu Ala Glu Glu Glu Glu Gly Glu Glu Glu65 70 75 80Met Asp Pro Met Lys Lys Met Gln Tyr Ala Ile Ala Ala Met Gln Pro 85 90 95Val Asp Leu Asp Pro Ala Thr Val Pro Lys Pro Asn Arg Arg Asn Val 100 105 110Arg Val Ser Asp Asp Pro Gln Thr Val Val Ala Arg Arg Arg Arg Glu 115 120 125Arg Ile Ser Glu Lys Ile Arg Ile Leu Lys Arg Met Val Pro Gly Gly 130 135 140Ala Lys Met Asp Thr Ala Ser Met Leu Asp Glu Ala Ile Arg Tyr Thr145 150 155 160Lys Phe Leu Lys Arg Gln Val Arg Leu Ala Ser Ser Ala Ser His Ser 165 170 175Ala Trp Ser Ser Tyr Val 1802185PRTBrassica napus 2Met Ser Gly Ser Lys Ala Asp Ala Ala Ile Ala Pro Ile Val Met Met1 5 10 15Glu His His His Leu Leu Met Asn Trp Asn Lys Pro Ile Asp Leu Ile 20 25 30Thr Glu Glu Asn Ser Phe Asn His Asn Pro His Phe Ile Val Asp Pro 35 40 45Pro Ser Glu Thr Leu Ser His Phe Gln Pro Pro Pro Thr Ile Phe Ser 50 55 60Asp His Gly Gly Gly Glu Glu Ala Glu Glu Glu Glu Glu Glu Glu Gly65 70 75 80Glu Glu Glu Met Asp Pro Met Lys Lys Met Gln Tyr Ala Ile Ala Ala 85 90 95Met Gln Pro Val Asp Leu Asp Pro Ala Thr Val Pro Lys Pro Asn Arg 100 105 110Arg Asn Val Arg Val Ser Asp Asp Pro Gln Thr Val Val Ala Arg Arg 115 120 125Arg Arg Glu Arg Ile Ser Glu Lys Ile Arg Ile Leu Lys Arg Met Val 130 135 140Pro Gly Gly Ala Lys Met Asp Thr Ala Ser Met Leu Asp Glu Ala Ile145 150 155 160Arg Tyr Thr Lys Phe Leu Lys Arg Gln Val Arg Leu Ala Ser Ser Ala 165 170 175Ser His Ser Ala Trp Ser Ser Tyr Val 180 1853182PRTBrassica napus 3Met Ser Gly Ser Lys Ala Asp Ala Ala Ile Ala Pro Ile Val Met Met1 5 10 15Glu His His His Leu Leu Met Asn Trp Asn Lys Pro Ile Asp Leu Ile 20 25 30Thr Glu Glu Asn Ser Phe Asn His Asn Pro His Phe Ile Val Asp Pro 35 40 45Pro Ser Glu Thr Leu Ser His Phe Gln Pro Pro Pro Thr Ile Phe Ser 50 55 60Asp His Gly Gly Gly Glu Glu Ala Glu Glu Glu Glu Gly Glu Glu Glu65 70 75 80Met Asp Pro Met Lys Lys Met Gln Tyr Ala Ile Ala Ala Met Gln Pro 85 90 95Val Asp Leu Asp Pro Ala Thr Val Pro Lys Pro Asn Arg Arg Asn Val 100 105 110Arg Val Ser Asp Asp Pro Gln Thr Val Val Ala Arg Arg Arg Arg Glu 115 120 125Arg Ile Ser Glu Lys Ile Arg Ile Leu Lys Arg Met Val Pro Gly Gly 130 135 140Ala Lys Met Asp Thr Ala Ser Met Leu Asp Glu Ala Ile Arg Tyr Thr145 150 155 160Lys Phe Leu Lys Arg Gln Val Arg Leu Ala Ser Ser Ala Ser His Ser 165 170 175Ala Trp Ser Ser Tyr Val 1804178PRTBrassica napus 4Met Ser Gly Ser Lys Ala Asp Ala Ala Ala Ile Ala Pro Ile Val Met1 5 10 15Met Glu Pro His His Leu Leu Met Asn Trp Asn Lys Pro Ile Asp Leu 20 25 30Ile Thr Gln Glu Asn Ser Phe Asn His Asn Pro His Phe Met Val Asp 35 40 45Pro Pro Ser Glu Thr Leu Ser His Phe Gln Pro Pro Pro Thr Glu Glu 50 55 60Ile Asp Glu Met Lys Glu Met Gln Tyr Ala Ile Ala Ala Met Gln Pro65 70 75 80Val Asp Ile Asp Pro Ala Thr Val Pro Lys Pro Asn Arg Arg Asn Val 85 90 95Arg Val Ser Glu Asp Pro Gln Thr Val Val Ala Arg Arg Arg Arg Glu 100 105 110Arg Ile Ser Glu Lys Ile Arg Ile Leu Lys Arg Met Val Pro Gly Gly 115 120 125Ala Lys Met Asp Thr Ala Ser Met Leu Asp Glu Ala Ile Arg Tyr Thr 130 135 140Lys Phe Leu Lys Arg Gln Val Arg Leu Leu Gln Pro His Thr Gln Leu145 150 155 160Gly Ala Pro Met Ser Asp Pro Ser Arg Leu Cys Tyr Tyr His Asn Ser 165 170 175Asp Thr5178PRTBrassica napus 5Met Ser Gly Ser Lys Ala Asp Ala Ala Ala Ile Ala Pro Ile Val Met1 5 10 15Met Glu Pro His His Leu Leu Met Asn Trp Asn Lys Pro Ile Asp Leu 20 25 30Ile Thr Gln Glu Asn Ser Phe Asn His Asn Pro His Phe Met Val Asp 35 40 45Pro Pro Ser Glu Thr Leu Ser His Phe Gln Pro Pro Pro Thr Glu Glu 50 55 60Ile Asp Glu Met Lys Glu Met Gln Tyr Ala Ile Ala Ala Met Gln Pro65 70 75 80Val Asp Ile Asp Pro Ala Thr Val Pro Lys Pro Asn Arg Arg Asn Val 85 90 95Arg Val Ser Glu Asp Pro Gln Thr Val Val Ala Arg Arg Arg Arg Glu 100 105 110Arg Ile Ser Glu Lys Ile Arg Ile Leu Lys Arg Met Val Pro Gly Gly 115 120 125Ala Lys Met Asp Thr Ala Ser Met Leu Asp Glu Ala Ile Arg Tyr Thr 130 135 140Lys Phe Leu Lys Arg Gln Val Arg Leu Leu Gln Pro His Thr Gln Leu145 150 155 160Gly Ala Pro Met Ser Asp Pro Ser Arg Leu Cys Tyr Tyr His Asn Ser 165 170 175Asp Thr6178PRTBrassica napus 6Met Ser Gly Ser Lys Ala Asp Ala Ala Ala Ile Ala Pro Ile Val Met1 5 10 15Met Glu Pro His His Leu Leu Met Asn Trp Asn Lys Pro Ile Asp Leu 20 25 30Ile Thr Gln Glu Asn Ser Phe Asn His Asn Pro His Phe Met Val Asp 35 40 45Pro Pro Ser Glu Thr Leu Ser His Phe Gln Pro Pro Pro Thr Glu Glu 50 55 60Ile Asp Glu Met Lys Glu Met Gln Tyr Ala Ile Ala Ala Met Gln Pro65 70 75 80Val Asp Ile Asp Pro Ala Thr Val Pro Lys Pro Asn Arg Arg Asn Val 85 90 95Arg Val Ser Glu Asp Pro Gln Thr Val Val Ala Arg Arg Arg Arg Glu 100 105 110Arg Ile Ser Glu Lys Ile Arg Ile Leu Lys Arg Met Val Pro Gly Gly 115 120 125Ala Lys Met Asp Thr Ala Ser Met Leu Asp Glu Ala Ile Arg Tyr Thr 130 135 140Lys Phe Leu Lys Arg Gln Val Arg Leu Leu Gln Pro His Thr Gln Leu145 150 155 160Gly Ala Pro Met Ser Asp Pro Ser Arg Leu Cys Tyr Tyr His Asn Ser 165 170 175Asp Thr7178PRTBrassica napus 7Met Ser Gly Ser Lys Ala Asp Ala Ala Ala Ile Ala Pro Ile Val Met1 5 10 15Met Glu Pro His His Leu Leu Met Asn Trp Asn Lys Pro Ile Asp Leu 20 25 30Ile Thr Gln Glu Asn Ser Phe Asn His Asn Pro His Phe Met Val Asp 35 40 45Pro Pro Ser Glu Thr Leu Ser His Phe Gln Pro Pro Pro Thr Glu Glu 50 55 60Ile Asp Glu Met Lys Glu Met Gln Tyr Ala Ile Ala Ala Met Gln Pro65 70 75 80Val Asp Ile Asp Pro Ala Thr Val Pro Lys Pro Asn Arg Arg Asn Val 85 90 95Arg Val Ser Glu Asp Pro Gln Thr Val Val Ala Arg Arg Arg Arg Glu 100 105 110Arg Ile Ser Glu Lys Ile Arg Ile Leu Lys Arg Met Val Pro Gly Gly 115 120 125Ala Lys Met Asp Thr Ala Ser Met Leu Asp Glu Ala Ile Arg Tyr Thr 130 135 140Lys Phe Leu Lys Arg Gln Val Arg Leu Leu Gln Pro His Thr Gln Leu145 150 155 160Gly Ala Pro Met Ser Asp Pro Ser Arg Leu Cys Tyr Tyr His Asn Ser 165 170 175Asp Thr8433PRTBrassica napus 8Met Ala His Cys Cys Thr Val Leu Thr Gly Leu Leu Val Trp Ser Leu1 5 10 15Phe Met Leu Ser Trp Cys Glu Ala Ser Arg Asn Asn Ile Asn Arg Tyr 20 25 30Asp His Ser Tyr Gly Thr Phe Lys Ser Asn Ser Leu Ile Lys Arg Arg 35 40 45Asp Asp Ile Thr Arg Leu Lys Ser Val Ala Arg Ala Ser Leu Arg Leu 50 55 60Pro Thr Thr Val Ser Val Ser Asp Phe Gly Ala Lys Gly Asp Gly Lys65 70 75 80Thr Asp Asp Thr Gln Ala Phe Val Asn Ala Trp Lys Lys Ala Cys Ser 85 90 95Ser Ser Gly Ala Val Asn Leu Leu Val Pro Glu Gly Lys Thr Tyr Phe 100 105 110Leu Lys Ser Leu Arg Leu Asn Gly Pro Cys Lys Ser Val Leu Thr Val 115 120 125Gln Ile Leu Gly Thr Leu Ser Ala Ser Gln Gln Arg Ser Asp Tyr Glu 130 135 140Asp Leu Ser Lys Trp Ile Thr Phe Asp Gly Val Asn Ser Leu Thr Val145 150 155 160Asp Gly Gly Ala Thr Gly Thr Val Asn Gly Asn Gly Glu Thr Trp Trp 165 170 175Glu Asn Ser Cys Lys Arg Asn Glu Ala Lys Lys Cys Thr Lys Ala Pro 180 185 190Thr Ala Leu Thr Phe Tyr Asn Ser Lys Asn Leu Arg Val Asn Asn Leu 195 200 205Arg Val Lys Asp Ala Gln Gln Ile Gln Ile Ser Ile Glu Lys Cys Ser 210 215 220Asn Val Gln Val Ser Asn Val Glu Val Thr Ala Pro Ala Asp Ser Pro225 230 235 240Asn Thr Asp Gly Ile His Ile Thr Asn Thr Gln Asn Ile Gln Val Ser 245 250 255Asn Ser Ile Ile Gly Thr Gly Asp Asp Cys Ile Ser Ile Glu Ser Gly 260 265 270Ser Gln Asn Val Lys Ile Asn Asp Leu Thr Cys Gly Ser Gly His Gly 275 280 285Ile Ser Ile Gly Ser Leu Gly Asp Asp Asn Ser Lys Ala Phe Val Ser 290 295 300Gly Val Thr Val Asp Gly Ala Lys Leu Ser Gly Thr Asp Asn Gly Val305 310 315 320Arg Ile Lys Thr Tyr Gln Gly Gly Ser Gly Thr Ala Ser Asn Ile Ile 325 330 335Phe Gln Asn Ile Gln Met Glu Asn Val Lys Asn Pro Ile Ile Ile Asp 340 345 350Gln Asp Tyr Cys Asp Lys Ser Lys Cys Thr Glu Gln Lys Ser Ala Val 355 360 365Gln Ile Lys Asn Val Val Tyr Arg Asn Ile Ser Gly Thr Ser Ala Ser 370 375 380Asp Ile Ala Ile Thr Phe Asn Cys Ser Lys Asn Tyr Pro Cys Gln Gly385 390 395 400Ile Val Leu Asp Lys Val Asn Ile Lys Gly Gly Lys Ala Ser Cys Ser 405 410 415Asn Ala Asn Val Met Asp Lys Asp Gly Val Leu Pro Gln Cys Lys Ser 420 425 430Thr9433PRTBrassica napus 9Met Ala His Cys Cys Thr Val Leu Thr Gly Leu Leu Val Trp Ser Leu1 5 10 15Phe Met Leu Ser Trp Cys Glu Ala Ser Arg Asn Asn Ile Asn Arg Tyr 20 25 30Asp His Ser Tyr Gly Thr Phe Lys Ser Asn Ser Leu Ile Lys Arg Arg 35 40 45Asp Asp Ile Thr Arg Leu Lys Ser Val Ala Arg Ala Ser Leu Arg Leu 50 55 60Pro Thr Thr Val Ser Val Ser Asp Phe Gly Ala Lys Gly Asp Gly Lys65 70 75 80Thr Asp Asp Thr Gln Ala Phe Val Asn Ala Trp Lys Lys Ala Cys Ser 85 90 95Ser Ser Gly Ala Val Asn Leu Leu Val Pro Glu Gly Lys Thr Tyr Phe 100 105 110Leu Lys Ser Leu Arg Leu Asn Gly Pro Cys Lys Ser Val Leu Thr Val 115 120 125Gln Ile Leu Gly Thr Leu Ser Ala Ser Gln Gln Arg Ser Asp Tyr Glu 130 135 140Asp Leu Ser Lys Trp Ile Thr Phe Asp Gly Val Asn Ser Leu Thr Val145 150 155 160Asp Gly Gly Ala Thr Gly Thr Val Asn Gly Asn Gly Glu Thr Trp Trp 165 170 175Glu Asn Ser Cys Lys Arg Asn Glu Ala Lys Lys Cys Thr Lys Ala Pro 180 185 190Thr Ala Leu Thr Phe Tyr Asn Ser Lys Asn Leu Arg Val Asn Asn Leu 195 200 205Arg Val Lys Asp Ala Gln Gln Ile Gln Ile Ser Ile Glu Lys Cys Ser 210 215 220Asn Val Gln Val Ser Asn Val Glu Val Thr Ala Pro Ala Asp Ser Pro225 230 235 240Asn Thr Asp Gly Ile His Ile Thr Asn Thr Gln Asn Ile Gln Val Ser 245 250 255Asn Ser Ile Ile Gly Thr Gly Asp Asp Cys Ile Ser Ile Glu Ser Gly 260 265 270Ser Gln Asn Val Lys Ile Asn Asp Leu Thr Cys Gly Pro Gly His Gly 275 280 285Ile Ser Ile Gly Ser Leu Gly Asp Asp Asn Ser Lys Ala Phe Val Ser 290 295 300Gly Val Thr Val Asp Gly Ala Lys Leu Ser Gly Thr Asp Asn Gly Val305 310 315 320Arg Ile Lys Thr Tyr Gln Gly Gly Ser Gly Thr Ala Ser Asn Ile Ile 325 330 335Phe Gln Asn Ile Gln Met Glu Asn Val Lys Asn Pro Ile Ile Ile Asp 340 345 350Gln Asp Tyr Cys Asp Lys Ser Lys Cys Thr Glu Gln Lys Ser Ala Val 355 360 365Gln Ile Lys Asn Val Val Tyr Arg Asn Ile Ser Gly Thr Ser Ala Ser 370 375 380Asp Ile Ala Ile Thr Phe Asn Cys Ser Lys Asn Tyr Pro Cys Gln Gly385 390 395 400Ile Val Leu Asp Lys Val Asn Ile Lys Gly Gly Lys Ala Ser Cys Ser 405 410 415Asn Ala Asn Val Met Asp Lys Asp Gly Val Leu Pro Gln Cys Lys Ser 420 425 430Thr10433PRTBrassica napus 10Met Ala His Cys Cys Thr Val Leu Thr Gly Leu Leu Val Trp Ser Leu1 5 10 15Phe Met Leu Ser Trp Cys Glu Ala Ser Arg Asn Asn Ile Asn Arg Tyr 20 25 30Asp His Ser Tyr Gly Thr Phe Lys Ser Asn Ser Leu Ile Lys Arg Arg 35 40 45Asp Asp Ile Thr Arg Leu Lys Ser Val Ala Arg Ala Ser Leu Arg Leu 50 55 60Pro Thr Thr Val Ser Val Ser Asp Phe Gly Ala Lys Gly Asp Gly Lys65 70 75 80Thr Asp Asp Thr Gln Ala Phe Val Asn Ala Trp Lys Lys Ala Cys Ser 85 90 95Ser Ser Gly Ala Val Asn Leu Leu Val Pro Glu Gly Lys Thr Tyr Phe 100 105 110Leu Lys Ser Leu Arg Leu Asn Gly Pro Cys Lys Ser Val Leu Thr Val 115 120 125Gln Ile Leu Gly Thr Leu Ser Ala Ser Gln Gln Arg Ser Asp Tyr Glu 130 135 140Asp Leu Ser Lys Trp Ile Thr Phe Asp Gly Val Asn Ser Leu Thr Val145 150 155 160Asp Gly Gly Ala Thr Gly Thr Val Asn Gly Asn Gly Glu Thr Trp Trp 165 170 175Glu Asn Ser Cys Lys Arg Asn Glu Ala Lys Lys Cys Thr Lys Ala Pro 180 185 190Thr Ala Leu Thr Phe Tyr Asn Ser Lys Asn Leu Arg Val Asn Asn Leu 195 200 205Arg Val Lys Asp Ala Gln Gln Ile Gln Ile Ser Ile Glu Lys Cys Ser 210 215 220Asn Val Gln Val Ser Asn Val Glu Val Thr Ala Pro Ala Asp Ser Pro225 230 235 240Asn Thr Asp Gly Ile His Ile Thr Asn Thr Gln Asn Ile Gln Val Ser 245 250 255Asn Ser Ile Ile Gly Thr Gly Asp Asp Cys Ile Ser Ile Glu Ser Gly 260 265 270Ser Gln Asn Val Lys Ile Asn Asp Leu Thr Cys Gly Pro Gly His Gly 275 280 285Ile Ser Ile Gly Ser Leu Gly Asp Asp Asn Ser Lys Ala Phe Val Ser 290 295 300Gly Val Thr Val Asp Gly Ala Lys Leu Ser Gly Thr Asp Asn Gly Val305 310 315 320Arg Ile Lys Thr Tyr Gln Gly Gly Ser Gly Thr Ala Ser Asn Ile Ile

325 330 335Phe Gln Asn Ile Gln Met Glu Asn Val Lys Asn Pro Ile Ile Ile Asp 340 345 350Gln Asp Tyr Cys Asp Lys Ser Lys Cys Thr Glu Gln Lys Ser Ala Val 355 360 365Gln Ile Lys Asn Val Val Tyr Arg Asn Ile Ser Gly Thr Ser Ala Ser 370 375 380Asp Ile Ala Ile Thr Phe Asn Cys Ser Lys Asn Tyr Pro Cys Gln Gly385 390 395 400Ile Val Leu Asp Lys Val Asn Ile Lys Gly Gly Lys Ala Ser Cys Ser 405 410 415Asn Ala Asn Val Met Asp Lys Asp Gly Val Leu Pro Gln Cys Lys Ser 420 425 430Thr11433PRTBrassica napus 11Met Ala His Cys Cys Thr Val Leu Thr Gly Leu Leu Val Trp Ser Leu1 5 10 15Phe Met Leu Ser Trp Cys Glu Ala Ser Arg Asn Asn Ile Asn Arg Tyr 20 25 30Asp His Ser Tyr Gly Thr Phe Lys Ser Asn Ser Leu Ile Lys Arg Arg 35 40 45Asp Asp Ile Thr Arg Leu Lys Ser Val Ala Arg Ala Ser Leu Arg Leu 50 55 60Pro Thr Thr Val Ser Val Ser Asp Phe Gly Ala Lys Gly Asp Gly Lys65 70 75 80Thr Asp Asp Thr Gln Ala Phe Val Asn Ala Trp Lys Lys Ala Cys Ser 85 90 95Ser Ser Gly Ala Val Asn Leu Leu Val Pro Glu Gly Lys Thr Tyr Phe 100 105 110Leu Lys Ser Leu Arg Leu Asn Gly Pro Cys Lys Ser Val Leu Thr Val 115 120 125Gln Ile Leu Gly Thr Leu Ser Ala Ser Gln Gln Arg Ser Asp Tyr Glu 130 135 140Asp Leu Ser Lys Trp Ile Thr Phe Asp Gly Val Asn Ser Leu Thr Val145 150 155 160Asp Gly Gly Ala Thr Gly Thr Val Asn Gly Asn Gly Glu Thr Trp Trp 165 170 175Glu Asn Ser Cys Lys Arg Asn Glu Ala Lys Lys Cys Thr Lys Ala Pro 180 185 190Thr Ala Leu Thr Phe Tyr Asn Ser Lys Asn Leu Arg Val Asn Asn Leu 195 200 205Arg Val Lys Asp Ala Gln Gln Ile Gln Ile Ser Ile Glu Lys Cys Ser 210 215 220Asn Val Gln Val Ser Asn Val Glu Val Thr Ala Pro Ala Asp Ser Pro225 230 235 240Asn Thr Asp Gly Ile His Ile Thr Asn Thr Gln Asn Ile Gln Val Ser 245 250 255Asn Ser Ile Ile Gly Thr Gly Asp Asp Cys Ile Ser Ile Glu Ser Gly 260 265 270Ser Gln Asn Val Lys Ile Asn Asp Leu Thr Cys Gly Pro Gly His Gly 275 280 285Ile Ser Ile Gly Ser Leu Gly Asp Asp Asn Ser Lys Ala Phe Val Ser 290 295 300Gly Val Thr Val Asp Gly Ala Lys Leu Ser Gly Thr Asp Asn Gly Val305 310 315 320Arg Ile Lys Thr Tyr Gln Gly Gly Ser Gly Thr Ala Ser Asn Ile Ile 325 330 335Phe Gln Asn Ile Gln Met Glu Asn Val Lys Asn Pro Ile Ile Ile Asp 340 345 350Gln Asp Tyr Cys Asp Lys Ser Lys Cys Thr Glu Gln Lys Ser Ala Val 355 360 365Gln Ile Lys Asn Val Val Tyr Arg Asn Ile Ser Gly Thr Ser Ala Ser 370 375 380Asp Ile Ala Ile Thr Phe Asn Cys Ser Lys Asn Tyr Pro Cys Gln Gly385 390 395 400Ile Val Leu Asp Lys Val Asn Ile Lys Gly Gly Lys Ala Ser Cys Ser 405 410 415Asn Ala Asn Val Met Asp Lys Asp Gly Val Leu Pro Gln Cys Lys Ser 420 425 430Thr12433PRTBrassica napus 12Met Ala His Cys Trp Thr Val Leu Thr Gly Leu Leu Val Trp Ser Leu1 5 10 15Leu Met Leu Ser Trp Cys Glu Ala Ser Arg Asn Asn Ile Asn Arg Tyr 20 25 30Asp His Ser Tyr Gly Thr Phe Lys Ser Asn Ser Leu Ile Lys Arg Arg 35 40 45Asp Asp Ile Thr Arg Leu Lys Ser Val Ala Arg Ala Ser Leu Arg Leu 50 55 60Pro Thr Thr Val Ser Val Ser Asp Phe Gly Ala Lys Gly Asp Gly Lys65 70 75 80Thr Asp Asp Thr Gln Ala Phe Val Asn Ala Trp Lys Lys Ala Cys Ser 85 90 95Ser Ser Gly Pro Val Asn Leu Leu Val Pro Glu Gly Lys Thr Tyr Phe 100 105 110Leu Lys Ser Leu Arg Leu Asn Gly Pro Cys Lys Ser Val Leu Thr Val 115 120 125Gln Ile Leu Gly Thr Leu Ser Ala Ser Gln Gln Arg Ser Asp Tyr Lys 130 135 140Asp Leu Ser Lys Trp Ile Thr Phe Asp Gly Val Asn Ser Leu Thr Val145 150 155 160Asp Gly Gly Ala Thr Gly Thr Val Asn Gly Asn Gly Glu Thr Trp Trp 165 170 175Glu Asn Ser Cys Lys Arg Asn Glu Ala Lys Lys Cys Thr Lys Ala Pro 180 185 190Thr Ala Leu Thr Phe Tyr Asn Ser Lys Asn Leu Arg Val Asn Asn Leu 195 200 205Arg Val Lys Asp Ala Gln Gln Ile Gln Ile Ser Ile Glu Lys Cys Ser 210 215 220Asn Val Gln Val Ser Asn Val Glu Val Thr Ala Pro Ala Asp Ser Pro225 230 235 240Asn Thr Asp Gly Ile His Ile Thr Asn Thr Gln Asn Ile Gln Val Ser 245 250 255Lys Ser Ile Ile Gly Thr Gly Asp Asp Cys Ile Ser Ile Glu Ser Gly 260 265 270Ser Gln Asn Val Lys Ile Asn Asp Leu Thr Cys Gly Pro Gly His Gly 275 280 285Ile Ser Ile Gly Ser Leu Gly Asp Asp Asn Ser Lys Ala Phe Val Ser 290 295 300Gly Val Thr Val Asp Gly Ala Lys Leu Ser Gly Thr Asp Asn Gly Val305 310 315 320Arg Ile Lys Thr Tyr Gln Gly Gly Ser Gly Thr Ala Ser Asn Ile Ile 325 330 335Phe Gln Asn Ile Gln Met Glu Asn Val Lys Asn Pro Ile Ile Ile Asp 340 345 350Gln Asp Tyr Cys Asp Lys Ser Lys Cys Thr Glu Gln Lys Ser Ala Val 355 360 365Gln Ile Lys Asn Val Val Tyr Arg Asn Ile Ser Gly Thr Ser Ala Ser 370 375 380Asp Ile Ala Ile Thr Phe Asn Cys Ser Lys Asn Tyr Pro Cys Gln Gly385 390 395 400Ile Val Leu Asp Lys Val Asn Ile Lys Gly Gly Lys Ala Ser Cys Ser 405 410 415Asn Ala Asn Val Met Asp Lys Asp Gly Val Leu Pro Gln Cys Lys Ser 420 425 430Thr13433PRTBrassica napus 13Met Ala His Cys Trp Thr Val Leu Thr Gly Leu Leu Val Trp Ser Leu1 5 10 15Leu Met Leu Ser Trp Cys Glu Ala Ser Arg Asn Asn Ile Asn Arg Tyr 20 25 30Asp His Ser Tyr Gly Thr Phe Lys Ser Asn Ser Leu Ile Lys Arg Arg 35 40 45Asp Asp Ile Thr Arg Leu Lys Ser Val Ala Arg Ala Ser Leu Arg Leu 50 55 60Pro Thr Thr Val Ser Val Ser Asp Phe Gly Ala Lys Gly Asp Gly Lys65 70 75 80Thr Asp Asp Thr Gln Ala Phe Val Asn Ala Trp Lys Lys Ala Cys Ser 85 90 95Ser Ser Gly Pro Val Asn Leu Leu Val Pro Glu Gly Lys Thr Tyr Phe 100 105 110Leu Lys Ser Leu Arg Leu Asn Gly Pro Cys Lys Ser Val Leu Thr Val 115 120 125Gln Ile Leu Gly Thr Leu Ser Ala Ser Gln Gln Arg Ser Asp Tyr Lys 130 135 140Asp Leu Ser Lys Trp Ile Thr Phe Asp Gly Val Asn Ser Leu Thr Val145 150 155 160Asp Gly Gly Ala Thr Gly Thr Val Asn Gly Asn Gly Glu Thr Trp Trp 165 170 175Glu Asn Ser Cys Lys Arg Asn Glu Ala Lys Lys Cys Thr Lys Ala Pro 180 185 190Thr Ala Leu Thr Phe Tyr Asn Ser Lys Asn Leu Arg Val Asn Asn Leu 195 200 205Arg Val Lys Asp Ala Gln Gln Ile Gln Ile Ser Ile Glu Lys Cys Ser 210 215 220Asn Val Gln Val Ser Asn Val Glu Val Thr Ala Pro Ala Asp Ser Pro225 230 235 240Asn Thr Asp Gly Ile His Ile Thr Asn Thr Gln Asn Ile Gln Val Ser 245 250 255Lys Ser Ile Ile Gly Thr Gly Asp Asp Cys Ile Ser Ile Glu Ser Gly 260 265 270Ser Gln Asn Val Lys Ile Asn Asp Leu Thr Cys Gly Pro Gly His Gly 275 280 285Ile Ser Ile Gly Ser Leu Gly Asp Asp Asn Ser Lys Ala Phe Val Ser 290 295 300Gly Val Thr Val Asp Gly Ala Lys Leu Ser Gly Thr Asp Asn Gly Val305 310 315 320Arg Ile Lys Thr Tyr Gln Gly Gly Ser Gly Thr Ala Ser Asn Ile Ile 325 330 335Phe Gln Asn Ile Gln Met Glu Asn Val Lys Asn Pro Ile Ile Ile Asp 340 345 350Gln Asp Tyr Cys Asp Lys Ser Lys Cys Thr Glu Gln Lys Ser Ala Val 355 360 365Gln Ile Lys Asn Val Val Tyr Arg Asn Ile Ser Gly Thr Ser Ala Ser 370 375 380Asp Ile Ala Ile Thr Phe Asn Cys Ser Lys Asn Tyr Pro Cys Gln Gly385 390 395 400Ile Val Leu Asp Lys Val Asn Ile Lys Gly Gly Lys Ala Ser Cys Ser 405 410 415Asn Ala Asn Val Met Asp Lys Asp Gly Val Leu Pro Gln Cys Lys Ser 420 425 430Thr14433PRTBrassica napus 14Met Ala His Cys Cys Thr Val Leu Thr Gly Leu Leu Val Trp Ser Leu1 5 10 15Leu Met Leu Ser Trp Cys Glu Ala Ser Arg Asn Asn Ile Asn Arg Tyr 20 25 30Asp His Ser Tyr Gly Thr Phe Lys Ser Asn Ser Leu Ile Lys Arg Arg 35 40 45Asp Asp Ile Thr Arg Leu Lys Ser Val Ala Arg Ala Ser Leu Gln Leu 50 55 60Pro Thr Thr Val Ser Val Ser Asp Phe Gly Ala Lys Gly Asp Gly Lys65 70 75 80Thr Asp Asp Thr Gln Ala Phe Val Asn Ala Trp Lys Lys Ala Cys Ser 85 90 95Ser Ser Gly Ala Val Asn Leu Leu Val Pro Glu Gly Lys Thr Tyr Phe 100 105 110Leu Lys Ser Ile Arg Leu Asn Gly Pro Cys Lys Ser Ile Leu Thr Val 115 120 125Gln Ile Leu Gly Thr Leu Ser Ala Ser Gln Gln Arg Ser Asp Tyr Lys 130 135 140Asp Leu Ser Lys Trp Ile Thr Phe Asp Gly Val Asn Ser Leu Thr Val145 150 155 160Asp Gly Gly Ala Thr Gly Thr Val Asn Gly Asn Gly Glu Thr Trp Trp 165 170 175Glu Asn Ser Cys Lys Arg Asn Glu Ala Lys Lys Cys Thr Lys Ala Pro 180 185 190Thr Ala Leu Thr Phe Tyr Asn Ser Lys Asn Leu Arg Val Asn Asn Leu 195 200 205Arg Val Lys Asp Ala Gln Gln Ile Gln Ile Ser Ile Glu Lys Cys Ser 210 215 220Asn Val Gln Val Ser Asn Val Glu Val Thr Ala Pro Ala Asp Ser Pro225 230 235 240Asn Thr Asp Gly Ile His Ile Thr Asn Thr Gln Asn Ile Gln Val Ser 245 250 255Lys Ser Ile Ile Gly Thr Gly Asp Asp Cys Ile Ser Ile Glu Ser Gly 260 265 270Ser Gln Asn Val Lys Ile Asn Asp Leu Thr Cys Gly Pro Gly His Gly 275 280 285Ile Ser Ile Gly Ser Leu Gly Asp Asp Asn Ser Lys Ala Phe Val Ser 290 295 300Gly Val Thr Val Asp Gly Ala Lys Leu Ser Gly Thr Asp Asn Gly Val305 310 315 320Arg Ile Lys Thr Tyr Gln Gly Gly Ser Gly Thr Ala Ser Asn Ile Ile 325 330 335Phe Gln Asn Ile Gln Met Glu Asn Val Lys Asn Pro Ile Ile Ile Asp 340 345 350Gln Asp Tyr Cys Asp Lys Ser Lys Cys Thr Glu Gln Lys Ser Ala Val 355 360 365Gln Ile Lys Asn Val Val Tyr Arg Asn Ile Ser Gly Thr Ser Ala Ser 370 375 380Asp Ile Ala Ile Thr Phe Asn Cys Ser Lys Asn Tyr Pro Cys Gln Gly385 390 395 400Ile Val Val Asp Lys Val Asn Ile Lys Gly Gly Lys Ala Ser Cys Ser 405 410 415Asn Ala Asn Val Met Asp Lys Asp Gly Val Leu Pro Gln Cys Lys Ser 420 425 430Thr15433PRTBrassica napus 15Met Ala His Cys Trp Thr Val Leu Thr Gly Leu Leu Val Trp Ser Leu1 5 10 15Leu Met Leu Ser Trp Cys Glu Ala Ser Arg Asn Asn Ile Asn Arg Tyr 20 25 30Asp His Ser Tyr Gly Thr Phe Lys Ser Asn Ser Leu Ile Lys Arg Arg 35 40 45Asp Asp Ile Thr Arg Leu Lys Ser Val Ala Arg Ala Ser Leu Arg Leu 50 55 60Pro Thr Thr Val Ser Val Ser Asp Phe Gly Ala Lys Gly Asp Gly Lys65 70 75 80Thr Asp Asp Thr Gln Ala Phe Val Asn Ala Trp Lys Lys Ala Cys Ser 85 90 95Ser Ser Gly Pro Val Asn Leu Leu Val Pro Glu Gly Lys Thr Tyr Phe 100 105 110Leu Lys Ser Leu Arg Leu Asn Gly Pro Cys Lys Ser Val Leu Thr Val 115 120 125Gln Ile Leu Gly Thr Leu Ser Ala Ser Gln Gln Arg Ser Asp Tyr Lys 130 135 140Asp Leu Ser Lys Trp Ile Thr Phe Asp Gly Val Asn Ser Leu Thr Val145 150 155 160Asp Gly Gly Ala Thr Gly Thr Val Asn Gly Asn Gly Glu Thr Trp Trp 165 170 175Glu Asn Ser Cys Lys Arg Asn Glu Ala Lys Lys Cys Thr Lys Ala Pro 180 185 190Thr Ala Leu Thr Phe Tyr Asn Ser Lys Asn Leu Arg Val Asn Asn Leu 195 200 205Arg Val Lys Asp Ala Gln Gln Ile Gln Ile Ser Ile Glu Lys Cys Ser 210 215 220Asn Val Gln Val Ser Asn Val Glu Val Thr Ala Pro Ala Asp Ser Pro225 230 235 240Asn Thr Asp Gly Ile His Ile Thr Asn Thr Gln Asn Ile Gln Val Ser 245 250 255Lys Ser Ile Ile Gly Thr Gly Asp Asp Cys Ile Ser Ile Glu Ser Gly 260 265 270Ser Gln Asn Val Lys Ile Asn Asp Leu Thr Cys Gly Pro Gly His Gly 275 280 285Ile Ser Ile Gly Ser Leu Gly Asp Asp Asn Ser Lys Ala Phe Val Ser 290 295 300Gly Val Thr Val Asp Gly Ala Lys Leu Ser Gly Thr Asp Asn Gly Val305 310 315 320Arg Ile Lys Thr Tyr Gln Gly Gly Ser Gly Thr Ala Ser Asn Ile Ile 325 330 335Phe Gln Asn Ile Gln Met Glu Asn Val Lys Asn Pro Ile Ile Ile Asp 340 345 350Gln Asp Tyr Cys Asp Lys Ser Lys Cys Thr Glu Gln Lys Ser Ala Val 355 360 365Gln Ile Lys Asn Val Val Tyr Arg Asn Ile Ser Gly Thr Ser Ala Ser 370 375 380Asp Ile Ala Ile Thr Phe Asn Cys Ser Lys Asn Tyr Pro Cys Gln Gly385 390 395 400Ile Val Leu Asp Lys Val Asn Ile Lys Gly Gly Lys Ala Ser Cys Ser 405 410 415Asn Ala Asn Val Met Asp Lys Asp Gly Val Leu Pro Gln Cys Lys Ser 420 425 430Thr16218PRTBrassica napus 16Met Gly Asn Ser Asp Glu Gly Asp Arg Leu Pro Ala Pro Ser Ser Ser1 5 10 15Asp Glu Leu Ser Ser Ile Leu Arg Gln Val Leu Ser Arg Thr Pro Thr 20 25 30Ala Gln Pro Ser Phe Ser Pro Lys Lys Ile Val Ser Ser Ala Glu Met 35 40 45Phe Asn Arg Thr Phe Pro Leu Leu Ile Pro Thr Pro Gly Gly Ala Val 50 55 60Ser Tyr Ala Ala Cys Ala Ala Ala Glu Thr Gly Glu Gly Lys Cys Gly65 70 75 80Phe Glu Asn Lys Arg Asn Gly Ala Lys Gln Arg Asn Ser Leu Lys Arg 85 90 95Asn Ile Asp Ala Gln Phe His Asn Leu Ser Glu Lys Arg Arg Arg Ser 100 105 110Lys Ile Asn Glu Lys Met Lys Ala Leu Gln Lys Leu Ile Pro Asn Ser 115 120 125Asn Lys Thr Asp Lys Ala Ser Met Leu Asp Glu Ala Ile Glu Tyr Leu 130 135 140Lys Gln Leu Gln Leu Gln Phe Gln Thr Leu Ala Ala Met Asn Gly Leu145 150 155 160Gly Leu Asn Pro Leu Arg Leu Pro Pro Ile Leu Pro Pro Thr Gln Thr 165 170 175Arg Ile Thr Gly Thr Ser Glu Gln Gly Leu Asn Leu Glu Thr Leu Leu 180 185 190Gly Gly Ser His Ser Met Ala Asn His Glu Pro Pro Glu Pro

Thr Gln 195 200 205Glu Met Cys Phe Ser Thr Thr Thr Leu Leu 210 21517214PRTBrassica napus 17Met Gly Asn Ser Asp Glu Gly Asp Arg Leu Pro Ala Pro Ser Ser Ser1 5 10 15Asp Glu Leu Ser Ser Ile Leu Arg Gln Val Leu Ser Arg Ala Pro Thr 20 25 30Ala Gln Pro Ser Phe Ser Pro Lys Lys Ile Val Ser Ser Ala Glu Met 35 40 45Phe Asn Arg Thr Phe Pro Leu Val Pro Gly Gly Ala Val Ser Tyr Ala 50 55 60Ala Cys Ala Ala Ala Glu Thr Gly Glu Gly Lys Cys Gly Phe Glu Asn65 70 75 80Lys Arg Asn Gly Ala Arg Gln Arg Asn Ser Leu Lys Arg Asn Ile Asp 85 90 95Ala Gln Phe His Asn Leu Ser Glu Lys Arg Arg Arg Ser Lys Ile Asn 100 105 110Glu Lys Met Lys Ala Leu Gln Lys Leu Ile Pro Asn Ser Asn Lys Thr 115 120 125Asp Lys Ala Ser Met Leu Asp Glu Ala Ile Glu Tyr Leu Lys Gln Leu 130 135 140Gln Leu Gln Phe Gln Thr Leu Ala Ala Met Asn Gly Leu Gly Leu Asn145 150 155 160Pro Leu Arg Leu Pro Pro Ile Leu Pro Pro Thr Gln Thr Arg Ile Thr 165 170 175Gly Thr Ser Glu Gln Gly Leu Asn Leu Glu Thr Leu Leu Gly Gly Ser 180 185 190His Ser Met Ala Asn Arg Glu Pro Pro Glu Thr Gln Glu Met Cys Phe 195 200 205Pro Thr Thr Thr Leu Leu 21018214PRTBrassica napus 18Met Gly Asn Ser Asp Glu Gly Gly Arg Leu Pro Ala Pro Ser Ser Ser1 5 10 15Asp Glu Leu Ser Ser Ile Leu Arg Gln Val Leu Ser Arg Thr Pro Thr 20 25 30Ala Gln Pro Ser Phe Ser Pro Lys Lys Ile Val Ser Ser Ala Glu Met 35 40 45Phe Asn Arg Thr Phe Pro Leu Val Pro Gly Gly Ala Val Ser Tyr Ala 50 55 60Ala Cys Ala Ala Ala Glu Thr Gly Glu Gly Lys Cys Gly Phe Glu Asn65 70 75 80Lys Arg Asn Gly Ala Lys Gln Arg Asn Ser Leu Lys Arg Asn Ile Asp 85 90 95Ala Gln Phe His Asn Leu Ser Glu Lys Arg Arg Arg Ser Lys Ile Asn 100 105 110Glu Lys Met Lys Ala Leu Gln Lys Leu Ile Pro Asn Ser Asn Lys Thr 115 120 125Asp Lys Ala Ser Met Leu Asp Glu Ala Ile Glu Tyr Leu Lys Gln Leu 130 135 140Gln Leu Gln Phe Gln Thr Leu Ala Ala Met Asn Gly Leu Gly Leu Asn145 150 155 160Pro Leu Arg Leu Pro Pro Ile Leu Pro Pro Thr Gln Thr Arg Ile Thr 165 170 175Gly Thr Ser Glu Gln Gly Leu Asn Leu Glu Thr Leu Leu Gly Gly Ser 180 185 190His Ser Met Ala Asn Arg Glu Pro Pro Glu Thr Gln Glu Met Cys Phe 195 200 205Pro Thr Thr Thr Leu Leu 21019216PRTBrassica napus 19Met Gly Asn Ser Asp Glu Gly Asp Arg Leu Pro Ala Pro Ser Ser Ser1 5 10 15Asp Glu Leu Ser Ser Ile Leu Arg Gln Val Leu Ser Arg Thr Pro Thr 20 25 30Ala Gln Pro Ser Phe Ser Pro Lys Lys Ile Val Ser Ser Ala Glu Met 35 40 45Phe Asn Arg Thr Phe Pro Leu Val Pro Gly Gly Ala Val Ser Tyr Ala 50 55 60Ala Cys Ala Val Ala Glu Thr Gly Glu Gly Lys Cys Gly Phe Glu Asn65 70 75 80Lys Arg Asn Gly Ala Arg Gln Arg Asn Ser Leu Lys Arg Asn Ile Asp 85 90 95Ala Gln Phe His Thr Leu Ser Glu Lys Arg Arg Arg Ser Lys Ile Asn 100 105 110Glu Lys Met Lys Ala Leu Gln Lys Leu Ile Pro Asn Ser Asn Lys Val 115 120 125Thr Asp Lys Ala Ser Met Leu Asp Glu Ala Ile Glu Tyr Leu Lys Gln 130 135 140Leu Gln Leu Gln Phe Gln Thr Leu Ala Ala Met Asn Gly Leu Gly Leu145 150 155 160Asn Pro Leu Arg Leu Pro Pro Ile Leu Pro Pro Thr Gln Thr Gly Ile 165 170 175Thr Gly Thr Ser Glu Gln Gly Leu Asn Leu Glu Thr Leu Leu Gly Gly 180 185 190Ser His Ser Met Ala Asn Leu Glu Pro Pro Glu Pro Thr Gln Glu Met 195 200 205Cys Phe Pro Thr Thr Thr Leu Leu 210 21520216PRTBrassica napus 20Met Gly Asn Ser Asp Glu Gly Asp Arg Leu Pro Ala Pro Ser Ser Ser1 5 10 15Asp Glu Leu Ser Ser Ile Leu Arg Gln Val Leu Ser Arg Thr Pro Thr 20 25 30Ala Gln Pro Ser Phe Ser Pro Lys Lys Ile Val Ser Ser Ala Glu Met 35 40 45Phe Asn Arg Thr Phe Pro Leu Val Pro Gly Gly Ala Val Ser Tyr Ala 50 55 60Ala Cys Ala Val Ala Glu Thr Gly Glu Gly Lys Cys Gly Phe Glu Asn65 70 75 80Lys Arg Asn Gly Ala Arg Gln Arg Asn Ser Leu Lys Arg Asn Ile Asp 85 90 95Ala Gln Phe His Thr Leu Ser Glu Lys Arg Arg Arg Ser Lys Ile Asn 100 105 110Glu Lys Met Lys Ala Leu Gln Lys Leu Ile Pro Asn Ser Asn Lys Val 115 120 125Thr Asp Lys Ala Ser Met Leu Asp Glu Ala Ile Glu Tyr Leu Lys Gln 130 135 140Leu Gln Leu Gln Phe Gln Thr Leu Ala Ala Met Asn Gly Leu Gly Leu145 150 155 160Asn Pro Leu Arg Leu Pro Pro Ile Leu Pro Pro Thr Gln Thr Gly Ile 165 170 175Thr Gly Thr Ser Glu Gln Gly Leu Asn Leu Glu Thr Leu Leu Gly Gly 180 185 190Ser His Ser Met Ala Asn Leu Glu Pro Pro Glu Pro Thr Gln Glu Met 195 200 205Cys Phe Pro Thr Thr Thr Leu Leu 210 21521216PRTBrassica napus 21Met Gly Asn Ser Asp Glu Gly Asp Arg Leu Pro Ala Pro Ser Ser Ser1 5 10 15Asp Glu Leu Ser Ser Ile Leu Arg Gln Val Leu Ser Arg Thr Pro Thr 20 25 30Ala Gln Pro Ser Phe Ser Pro Lys Lys Ile Val Ser Ser Ala Glu Met 35 40 45Phe Asn Arg Thr Phe Pro Leu Val Pro Gly Gly Ala Val Ser Tyr Ala 50 55 60Ala Cys Ala Val Ala Glu Thr Gly Glu Gly Lys Cys Gly Phe Glu Asn65 70 75 80Lys Arg Asn Gly Ala Arg Gln Arg Asn Ser Leu Lys Arg Asn Ile Asp 85 90 95Ala Gln Phe His Thr Leu Ser Glu Lys Arg Arg Arg Ser Lys Ile Asn 100 105 110Glu Lys Met Lys Ala Leu Gln Lys Leu Ile Pro Asn Ser Asn Lys Val 115 120 125Thr Asp Lys Ala Ser Met Leu Asp Glu Ala Ile Glu Tyr Leu Lys Gln 130 135 140Leu Gln Leu Gln Phe Gln Thr Leu Ala Ala Met Asn Gly Leu Gly Leu145 150 155 160Asn Pro Leu Arg Leu Pro Pro Ile Leu Pro Pro Thr Gln Thr Gly Ile 165 170 175Thr Gly Thr Ser Glu Gln Gly Leu Asn Leu Glu Thr Leu Leu Gly Gly 180 185 190Ser His Ser Met Ala Asn Leu Glu Pro Pro Glu Pro Thr Gln Glu Met 195 200 205Cys Phe Pro Thr Thr Thr Leu Leu 210 21522673DNABrassica napus 22ttgtttcatg atgctgaata tttgaatgtg ttagtattgc taacattggt ttcttttgtt 60caagtatcgg tattcggtag tttaataaaa acaaatatgg aagtttcata acaaaaaaaa 120aagtctacca aaatttatgc aagttaatta tcattcaaga caacacccac tgcaatttgt 180caaaaatggt gaaactcttg caagacctta cctgtgaagt gattgtcctt tttcatgttt 240aataataata attaattaca gtattattta tgactttcag aaaactctat ctatcaaccg 300ttctttactg atttttccac ccatcactgc tctatttttt atttatggca tcgggaaaaa 360aagagaagaa aaagaaatat tatatgcaat cttttttttt ttttatgcaa tcttaccggg 420tgttaatact tataactaca tttagtgata aatctgcttt gttaaatcca gtacaaaatc 480aattattgaa tttatagact ataaagttgt gaacaaaata caaattatca ctaatgggaa 540tattcatata cagtataata acgtaagcca ccagattatg gctagagtga tttgccacgc 600gcctgcctat ttattatgaa aagcctcagt aactttgtga tgagaagaat tcacagagag 660agagaggaga gag 67323677DNABrassica napus 23ttgtttcatg atgctgaata tttgaatgtg ttagtattgc taacattggt ttcttttgtt 60caagtatcgg tattcggtag tttaataaaa acaaatatgg aagtttcata acaaaaaaaa 120aagtctacca aaatttatgc aagttaatta tcattcaaga caacacccac tgcaatttgt 180caaaaatggt gaaactcttg caagacctta cctgtgaagt gattgtcctt tttcatgttt 240aataataata attaattaca gtattattta tgactttcag aaaactctat ctatcaaccg 300ttctttactg atttttccac ccatcactgc tctatttttt atttatggca tcgggaaaaa 360aagagaagaa aaagaaatat tatatgcaat cttttttttt ttttttttat gcaatcttac 420cgggtgttaa tacttataac tacatttagt gataaatctg ctttgttaaa tccagtacaa 480aatcaattat tgaatttata gactataaag ttgtgaacaa aatacaaatt atcactaatg 540ggaatattca tatacagtat aataacgtaa gccaccagat tatggctaga gtgatttgcc 600acgcgcctgc ctatttatta tgaaaagcct cagtaactct gtgacgagaa gaattcacag 660agagagagag gagagag 677245224DNABrassica napus 24gtgtttcatt atgctgaata tttgaatgtg ttagtattgc caacattggt ttcttttgtt 60caagtatcgg tattcggtag tttaatgaat acaaatatgg aagtttcata acaaaaaaaa 120aagtctaccg aaatttatgc aagttaatta tcattcaaga caacacccac tgcaatttgt 180caaaaatggt gaaactcttg caagacctta cctgtgaagt gattgtcctt tttcatgttt 240aataataata attaattaca gtattatttg aaactctttg taaacttaaa gtacttttct 300tattgaatga aggcaacata agagttgctt aaataacaaa gcgtacagat actcccgaca 360gttttggtaa agtcttaacc acttcctata gtagaggaag acttattcaa ataacttaaa 420gcagaaaata attcaaaact accaactaaa gactctacca actaaagact cgatcttttt 480tattcattaa tccctccctt aaacttgaac tcctttataa agtttaaact tcctttaagc 540tgactcccat gtgtgctctc agcttctcaa acgcttcaag tttcaccggc tttgtcatga 600tatccgacaa ttgcttatga gttgaacaat actccattga gatgatgcct tcattcacaa 660gctctcttaa gaaatgaaac ctcacatgaa tatgcttgct tcttccatgt agaacaggat 720tccttgacaa cttgatagct gaactattat cacagaaaag aacagttcca tcaccttgat 780caaacccaat ttcagccaac acgttcctca tccatacagc ttgacaagct ccaattgctg 840cagcaacata ctctgcttcc gtggtagaga gtgtgacaat tggttgtttc tttgagctcc 900aggacacagc tcctcctcca aacatgaaag tgtagcctga tgtactcttt ctgtcatcct 960catcgcctgc ataatcactg tccacatacc ctacaagctt ttcttctcca ccacgcaagt 1020actgaatacc atagcttgca gttccttgca catacctcaa aatacgctta acagccatca 1080tgtgcccttc atttggattc tccatgtatc tactaaccac attcacagag tatatcagat 1140caggtcgagt agctgttaag tacctcaagc ttcccacgag ttgcttgtaa gcggttggat 1200caacttcttc tccagctcct gtcttggtca gtttgttccc tggaaccatt ggattcttca 1260cagagttgca gtctttcatt ccatacttct ttaaagtctc cagtgcatac ttcttttggc 1320caataaagat cccaacctcg tcttgcacaa cctccacgcc tagaaagtac ttcatctttc 1380ctagatcagt cattgagaac tcttcttgca ttgaagtttt gaagctctcc agcatgcttt 1440ccgagttact agtgtaaatg aggtcgtcta cgtacaagct caggatcaac gagttccctt 1500cttctgtttt tacgaacaat gtgtgttcgc aataacattt ctcaaagcct tcctttataa 1560agtaactctc gatcctgcta taccaggccc taggagcttg tcttaatcca tacaaagcct 1620ttttcaactt gtagaccttc tcactgtcaa cctgagactc gaacccctta ggctgctcaa 1680cgtaaacgtc ctccttaagc tcaccatgta ggaacgcgct cttcacatct agttgatata 1740cactccatct tctttgagcc gctaagccga gaatcagcct gatcgtgtcc catctggcca 1800caggtgcaaa aacttcatgg aagtctactc cataggtttg gtgaaagccc tttgccacca 1860gtctcgcttt gtacttatcc acctctcctt tttcattcaa cttggtcttg aagacccatt 1920tcactcctat caccttcaca ttggcaggta gactaaccag ctcccaagtg ttgttatcct 1980ctattgattc aatctcagtt tccattgcct ttctccattt gtcatgttgg atagcatctt 2040caaacttctc tggatcctca tctgacaaga acagcgcaac tagttcttct ccatcatctt 2100ccatgataag acccctccca tcaaccacgt aatctttcat ccagactggt cttagtttgc 2160ttcttcctgc tctgcttgtg tctgatcttt cttctggtgt agagacagtt ctttctccat 2220cagactcagt gttatctcct ccttctgtct cttcgttagt tgctggtgct gtcaccaggt 2280tctcaccctg attcctctct tcctcttctt cagcttctcc acattcgaca aatttgtctt 2340catcaatgtt atcttcccag ttccaccgcg tttcttcgtc aaacctcacg tctttgctta 2400ttatgatcct cttcccaata ggatcataaa gtctgtaagc cttcgactcc ttgcttacac 2460caaacatgac acatctcaca cttttctcat ccagcttcac acgcttctca taaggtataa 2520gtgcataagc cacacagcca aacacacgga gatgctctac agacggcttg tgcttactcc 2580acttctcact tggcgtcact tctcccaata tctttgttgg acttctgtta agtatatgga 2640cagcatactg tgttgcttct gtccaatact tgatcggcac cttcatacca aacagcatgc 2700acctgaccat attcagaatg cttctattct tcctctctgc cacgccgttc tgctgaggcg 2760tgtatgcggc tgtcagctgt cgtttgatgc cgtgctccac acaatagtcc tgaaacgcct 2820ttgaattgta ttctccacct ctgtcggttc ttagacagac gagcagctca ccactctccc 2880tctcagcggc atttttgaac tccttgaaag ttctaagtgc ctctgatttc tctgctagaa 2940agaagctcca acactttcgg ctaaactcat caatgaaatt gattatgtac ctcttgccgc 3000tttcagatgt gggtgatatt ggcccgcaga tgtcactgtg aactagctcc agtcgttgtg 3060ttgctctcca tagactcttc tttggtatct tttctctgat ctgcttcccc ttcatacaca 3120cctcacatac tgtcccgttg gtgttgatct ttggtagacc ttccaccatc gccttttctg 3180ctagtgttag caaactgttg tggttcaaat gtccgaagcg tttgtgccaa gtttcctcca 3240ttgtctccat cgtattctga acttggtttg cctccaacgc ttccttaggc tccctgacaa 3300tagcagttac tacaaacatc ctgtttgtgg tcatcagaga atgcatgatc agtctccgtt 3360gttgtttgtg ccagatctca cacgtatctc cttcaattat aatcctcaac cccttctgtt 3420gcaactgacc gacagacagt agattgttct tcaagccagg cacaaagtat actgcagtga 3480tcacttgagt agtaccattg atctccaatc ttaggttacc tcttccctca acttgcattc 3540gcctatcatc tccaagcttt acttgttgtc tgaactggct atcaaactct gaaaaccact 3600ccttggttcc acacatgtgg ttgctacacc ctgagtcaag gtaccacacg tgtctttctt 3660cagtctcaac ttgagtcatc agcaaaatat cctcctctaa ctctgcatag ttagctgcct 3720tctcccagct aggacattcg tttttgaaat gccccatctt gtggcactta aagcactcaa 3780tgttgctctt gtcaaagctt gtatttcctc tgtcttgtcc tccacgacct cctctgtaac 3840tgcctcttcc tctggtttga tatcctcctc gtccattgga gtaacctgag tagccacctc 3900gtccccttcc tccttccatc ttcaggactt gttcctctcc tgtgtgttta ctcaggttct 3960gctcatgtac gagtaatgag ctttgtaact catccaccga cagctccttg atatcctttg 4020attcctctat cgcacatacc acatatgtga acttctccac aagggttctt aggatcttct 4080caaccacctt ttcatctggc atgtcttctc cacggttccg catgtcatta gcaaccacca 4140tgactctaga gaaatagtct gcgattgagt caccttcctt catctccagg acttcaaagt 4200ttcttctcag cctctgtaat tgcgcacttt gaaccctctt gttgccttgg tactttgcct 4260tcagcgactc ccatatgtct tttgcggtgt ccttcttcac aatcgtcttc agaacagtct 4320tgtcgatgga ggcaaagaga tagttcttca ccttgaagtc tttgagcttt tgctccgcaa 4380gctcggttct ctgagcttca gacagtgtca cactactttc atgttgaatg attccggtat 4440tgatcagttc ccaccactcc ttggatctca acaaattctc cataagcatt gcccaatgct 4500catagtctcc atcaagcttt ggtatgctca acgaggactc cttttcactc atctctctta 4560ctttcggatt acctagaact cacaacagtt tttgttagag ctctgatacc acttgaaact 4620ctttgtaaac ttaaagtatt tttcttattg aatgaaggca acataagagt tgcttaaata 4680acaaagcgta cagatactcc cgacagtttt ggtaaagtct taaccacttc ctatagtaga 4740ggaagactta ttcaaataac ttaaagcaga aaataattca aaactaccaa ctaaagactc 4800taccaactaa agactcgatc ttttttattc attattattt atgactttca gaaaactcta 4860tctatcaacc gttctttact gatttttcca cccatcactg ctctattttt tatttattta 4920tggcatcggg aaaaaaagag aagaaaaaga aaaattatat gcaattttac cgggtgttaa 4980aacttataat tacatttggt gataaatctg ctttgttaaa tccagtacaa aatcaattat 5040tgaattcata gactataaag ttgtgaacca aatacaaatt atcactaatg ggaatattca 5100tatacagtat agtaacgtaa gccaccagat tatgtctaga gtgatttgcc acgcgcctgc 5160ctatttatta tgaaaagcct cagtaacttg tgatgagaag aattcacaga gagagaggag 5220agag 5224255224DNABrassica napus 25gtgtttcatt atgctgaata tttgaatgtg ttagtattgc caacattggt ttcttttgtt 60caagtatcgg tattcggtag tttaatgaat acaaatatgg aagtttcata acaaaaaaaa 120aagtctaccg aaatttatgc aagttaatta tcattcaaga caacacccac tgcaatttgt 180caaaaatggt gaaactcttg caagacctta cctgtgaagt gattgtcctt tttcatgttt 240aataataata attaattaca gtattatttg aaactctttg taaacttaaa gtacttttct 300tattgaatga aggcaacata agagttgctt aaataacaaa gcgtacagat actcccgaca 360gttttggtaa agtcttaacc acttcctata gtagaggaag acttattcaa ataacttaaa 420gcagaaaata attcaaaact accaactaaa gactctacca actaaagact cgatcttttt 480tattcattaa tccctccctt aaacttgaac tcctttataa agtttaaact tcctttaagc 540tgactcccat gtgtgctctc agcttctcaa acgcttcaag tttcaccggc tttgtcatga 600tatccgacaa ttgcttatga gttgaacaat actccattga gatgatgcct tcattcacaa 660gctctcttaa gaaatgaaac ctcacatgaa tatgcttgct tcttccatgt agaacaggat 720tccttgacaa cttgatagct gaactattat cacagaaaag aacagttcca tcaccttgat 780caaacccaat ttcagccaac acgttcctca tccatacagc ttgacaagct ccaattgctg 840cagcaacata ctctgcttcc gtggtagaga gtgtgacaat tggttgtttc tttgagctcc 900aggacacagc tcctcctcca aacatgaaag tgtagcctga tgtactcttt ctgtcatcct 960catcgcctgc ataatcactg tccacatacc ctacaagctt ttcttctcca ccacgcaagt 1020actgaatacc atagcttgca gttccttgca catacctcaa aatacgctta acagccatca 1080tgtgcccttc atttggattc tccatgtatc tactaaccac attcacagag tatatcagat 1140caggtcgagt agctgttaag tacctcaagc ttcccacgag ttgcttgtaa gcggttggat 1200caacttcttc tccagctcct gtcttggtca gtttgttccc tggaaccatt ggattcttca 1260cagagttgca gtctttcatt ccatacttct ttaaagtctc cagtgcatac ttcttttggc 1320caataaagat cccaacctcg tcttgcacaa cctccacgcc tagaaagtac ttcatctttc 1380ctagatcagt cattgagaac tcttcttgca ttgaagtttt gaagctctcc agcatgcttt 1440ccgagttact agtgtaaatg aggtcgtcta cgtacaagct caggatcaac gagttccctt 1500cttctgtttt tacgaacaat gtgtgttcgc aataacattt ctcaaagcct tcctttataa

1560agtaactctc gatcctgcta taccaggccc taggagcttg tcttaatcca tacaaagcct 1620ttttcaactt gtagaccttc tcactgtcaa cctgagactc gaacccctta ggctgctcaa 1680cgtaaacgtc ctccttaagc tcaccatgta ggaacgcgct cttcacatct agttgatata 1740cactccatct tctttgagcc gctaagccga gaatcagcct gatcgtgtcc catctggcca 1800caggtgcaaa aacttcatgg aagtctactc cataggtttg gtgaaagccc tttgccacca 1860gtctcgcttt gtacttatcc acctctcctt tttcattcaa cttggtcttg aagacccatt 1920tcactcctat caccttcaca ttggcaggta gactaaccag ctcccaagtg ttgttatcct 1980ctattgattc aatctcagtt tccattgcct ttctccattt gtcatgttgg atagcatctt 2040caaacttctc tggatcctca tctgacaaga acagcgcaac tagttcttct ccatcatctt 2100ccatgataag acccctccca tcaaccacgt aatctttcat ccagactggt cttagtttgc 2160ttcttcctgc tctgcttgtg tctgatcttt cttctggtgt agagacagtt ctttctccat 2220cagactcagt gttatctcct ccttctgtct cttcgttagt tgctggtgct gtcaccaggt 2280tctcaccctg attcctctct tcctcttctt cagcttctcc acattcgaca aatttgtctt 2340catcaatgtt atcttcccag ttccaccgcg tttcttcgtc aaacctcacg tctttgctta 2400ttatgatcct cttcccaata ggatcataaa gtctgtaagc cttcgactcc ttgcttacac 2460caaacatgac acatctcaca cttttctcat ccagcttcac acgcttctca taaggtataa 2520gtgcataagc cacacagcca aacacacgga gatgctctac agacggcttg tgcttactcc 2580acttctcact tggcgtcact tctcccaata tctttgttgg acttctgtta agtatatgga 2640cagcatactg tgttgcttct gtccaatact tgatcggcac cttcatacca aacagcatgc 2700acctgaccat attcagaatg cttctattct tcctctctgc cacgccgttc tgctgaggcg 2760tgtatgcggc tgtcagctgt cgtttgatgc cgtgctccac acaatagtcc tgaaacgcct 2820ttgaattgta ttctccacct ctgtcggttc ttagacagac gagcagctca ccactctccc 2880tctcagcggc atttttgaac tccttgaaag ttctaagtgc ctctgatttc tctgctagaa 2940agaagctcca acactttcgg ctaaactcat caatgaaatt gattatgtac ctcttgccgc 3000tttcagatgt gggtgatatt ggcccgcaga tgtcactgtg aactagctcc agtcgttgtg 3060ttgctctcca tagactcttc tttggtatct tttctctgat ctgcttcccc ttcatacaca 3120cctcacatac tgtcccgttg gtgttgatct ttggtagacc ttccaccatc gccttttctg 3180ctagtgttag caaactgttg tggttcaaat gtccgaagcg tttgtgccaa gtttcctcca 3240ttgtctccat cgtattctga acttggtttg cctccaacgc ttccttaggc tccctgacaa 3300tagcagttac tacaaacatc ctgtttgtgg tcatcagaga atgcatgatc agtctccgtt 3360gttgtttgtg ccagatctca cacgtatctc cttcaattat aatcctcaac cccttctgtt 3420gcaactgacc gacagacagt agattgttct tcaagccagg cacaaagtat actgcagtga 3480tcacttgagt agtaccattg atctccaatc ttaggttacc tcttccctca acttgcattc 3540gcctatcatc tccaagcttt acttgttgtc tgaactggct atcaaactct gaaaaccact 3600ccttggttcc acacatgtgg ttgctacacc ctgagtcaag gtaccacacg tgtctttctt 3660cagtctcaac ttgagtcatc agcaaaatat cctcctctaa ctctgcatag ttagctgcct 3720tctcccagct aggacattcg tttttgaaat gccccatctt gtggcactta aagcactcaa 3780tgttgctctt gtcaaagctt gtatttcctc tgtcttgtcc tccacgacct cctctgtaac 3840tgcctcttcc tctggtttga tatcctcctc gtccattgga gtaacctgag tagccacctc 3900gtccccttcc tccttccatc ttcaggactt gttcctctcc tgtgtgttta ctcaggttct 3960gctcatgtac gagtaatgag ctttgtaact catccaccga cagctccttg atatcctttg 4020attcctctat cgcacatacc acatatgtga acttctccac aagggttctt aggatcttct 4080caaccacctt ttcatctggc atgtcttctc cacggttccg catgtcatta gcaaccacca 4140tgactctaga gaaatagtct gcgattgagt caccttcctt catctccagg acttcaaagt 4200ttcttctcag cctctgtaat tgcgcacttt gaaccctctt gttgccttgg tactttgcct 4260tcagcgactc ccatatgtct tttgcggtgt ccttcttcac aatcgtcttc agaacagtct 4320tgtcgatgga ggcaaagaga tagttcttca ccttgaagtc tttgagcttt tgctccgcaa 4380gctcggttct ctgagcttca gacagtgtca cactactttc atgttgaatg attccggtat 4440tgatcagttc ccaccactcc ttggatctca acaaattctc cataagcatt gcccaatgct 4500catagtctcc atcaagcttt ggtatgctca acgaggactc cttttcactc atctctctta 4560ctttcggatt acctagaact cacaacagtt tttgttagag ctctgatacc acttgaaact 4620ctttgtaaac ttaaagtatt tttcttattg aatgaaggca acataagagt tgcttaaata 4680acaaagcgta cagatactcc cgacagtttt ggtaaagtct taaccacttc ctatagtaga 4740ggaagactta ttcaaataac ttaaagcaga aaataattca aaactaccaa ctaaagactc 4800taccaactaa agactcgatc ttttttattc attattattt atgactttca gaaaactcta 4860tctatcaacc gttctttact gatttttcca cccatcactg ctctattttt tatttattta 4920tggcatcggg aaaaaaagag aagaaaaaga aaaattatat gcaattttac cgggtgttaa 4980aacttataat tacatttggt gataaatctg ctttgttaaa tccagtacaa aatcaattat 5040tgaattcata gactataaag ttgtgaacca aatacaaatt atcactaatg ggaatattca 5100tatacagtat agtaacgtaa gccaccagat tatgtctaga gtgatttgcc acgcgcctgc 5160ctatttatta tgaaaagcct cagtaacttg tgatgagaag aattcacaga gagagaggag 5220agag 52242620DNAArtificial Sequencesynthesized oligonucleotide 26gagcgactta tgcttctata 202720DNAArtificial Sequencesynthesized oligonucleotide 27gagaaaggct tggttctccc 202820DNAArtificial Sequencesynthesized oligonucleotide 28gtttcttgcc gaatatatga 202920DNAArtificial Sequencesynthesized oligonucleotide 29ggctatatat tgccgcagct 203020DNAArtificial Sequencesynthesized oligonucleotide 30ggtcattctg accaattatc 203120DNAArtificial Sequencesynthesized oligonucleotide 31gaacgtaagc caccagatta 203220DNAArtificial Sequencesynthesized oligonucleotide 32gtggagagga aatctgactt 203320DNAArtificial Sequencesynthesized oligonucleotide 33ggaagaattc acagagagag 203420DNAArtificial Sequencesynthesized oligonucleotide 34ggactgaaca aaactgctac 203520DNAArtificial Sequencesynthesized oligonucleotide 35gcaactaacg aagagacaga 2036597DNAArabidopsis thaliana 36atggaaaatg gtatgtataa aaagaaagga gtgtgcgact cttgtgtctc gtccaaaagc 60agatccaacc acagccccaa aagaagcatg atggagcctc agcctcacca tctcctcatg 120gattggaaca aagctaatga tcttctcaca caagaacacg cagcttttct caatgatcct 180caccatctca tgttagatcc acctcccgaa accctaattc acttggacga agacgaagag 240tacgatgaag acatggatgc gatgaaggag atgcagtaca tgatcgccgt catgcagccc 300gtagacatcg accctgccac ggtccctaag ccgaaccgcc gtaacgtaag gataagcgac 360gatcctcaga cggtggttgc tcgtcggcgt cgggaaagga tcagcgagaa gatccgaatt 420ctcaagagga tcgtgcctgg tggtgcgaag atggacacag cttccatgct cgacgaagcc 480atacgttaca ccaagttctt gaaacggcag gtgaggattc ttcagcctca ctctcagatt 540ggagctccta tggctaaccc ctcttacctt tgttattacc acaactccca accctga 5973720DNAArtificial Sequencesynthesized oligonucleotide 37gcccatggat aaatgtgcct 203820DNAArtificial Sequencesynthesized oligonucleotide 38gtccaaactg tctacaccga 203920DNAArtificial Sequencesynthesized oligonucleotide 39gtgaagcagg gtcgaccttt 204020DNAArtificial Sequencesynthesized oligonucleotide 40ggggaatcag tatattagct 204120DNAArtificial Sequencesynthesized oligonucleotide 41ggattgactg tctaattggg 204220DNAArtificial Sequencesynthesized oligonucleotide 42gctgattaaa ttgttcatgc 2043633DNAArabidopsis thaliana 43atgggtgatt ctgacgtcgg tgatcgtctt ccccctccat cttcttccga cgaactctcg 60agctttctcc gacagattct ttcccgtact cctacagctc aaccttcttc accaccgaag 120agtactaatg tttcctccgc tgagaccttc ttcccttccg tttccggcgg agctgtttct 180tccgtcggtt atggagtctc tgaaactggc caagacaaat atgctttcga acacaagaga 240agtggagcta aacagagaaa ttcgttgaag agaaacattg atgctcaatt ccacaacttg 300tctgaaaaga agaggaggag caagatcaac gagaaaatga aagctttgca gaaactcatt 360cccaattcca acaagactga taaagcctca atgcttgatg aagctataga atatctgaag 420cagcttcaac ttcaagtcca gactttagcc gttatgaatg gtttaggctt aaaccctatg 480cgattaccac aggttccacc tccaactcat acaaggatca atgagacctt agagcaagac 540ctgaacctag agactcttct cgctgctcct cactcgctgg aaccagctaa aacaagtcaa 600ggaatgtgct tttccacagc cactctgctt tga 6334420DNAArtificial Sequencesynthesized oligonucleotide 44gtctctgtgc cctggctccc 204520DNAArtificial Sequencesynthesized oligonucleotide 45gccagcctac gctggtggac 204620DNAArtificial Sequencesynthesized oligonucleotide 46gagaccggtg tgcttatgga 204720DNAArtificial Sequencesynthesized oligonucleotide 47gcgttcttca gagaagcgcg 204820DNAArtificial Sequencesynthesized oligonucleotide 48ggacaccaga ttctgtctat 204920DNAArtificial Sequencesynthesized oligonucleotide 49gtcttcagag aagcgcgtgg 205020DNAArtificial Sequencesynthesized oligonucleotide 50gtgaaatcca acgagataaa 205120DNAArtificial Sequencesynthesized oligonucleotide 51gaacttgcca tctaaataaa 205219DNAArtificial Sequencesynthesized oligonucleotide 52cacatgcacg gatttttgg 195321DNAArtificial Sequencesynthesized oligonucleotide 53gattcatcaa actcgatgca c 215422DNAArtificial Sequencesynthesized oligonucleotide 54ttcctctcac tctccagctt ac 225519DNAArtificial Sequencesynthesized oligonucleotide 55atggtgaaac tgttgcgag 195615000DNABrassica napus 56gtagttccaa tactaaatcc tcaactctaa tcagtgaacc ctaaacttta tcccaacccc 60accccatccc ctaaaatact aaaccctaaa cactaatcag cgaacctaaa cccaaatata 120aaccataaat ccaaatataa accctaaacc caaatagaat ggaacaacta tgaacccaaa 180tatcaaaatc taaaccttaa ccagtgttta accccaattt tccataaact aaattcaaat 240tttaaaatgc cagtaaaata cattaccatt ctacatgagt catatagaat agaaatagag 300aaaatgtagt tccaatacta aatcctcaac tctaatcagt gaaccctaaa ctttatccca 360accctacccc atcccctaaa atactaaacc ctaaactcta atcagcgaac ctaaacccaa 420atataaacca taaatccaaa tataaaccct aaacccaaat agaatggaac aaaataaata 480gaatagtaca tactggtgaa attatgaaat atctatgatt gcatgaaaga agttaataat 540gactccaacc ataacccctc accttctaat tagtaaaccc taaactataa tcactaaacc 600ctaaacgcaa atatcaaaat ataaaaatgt atgcaatatt atgcaatatt aaatcatatt 660atataatatt aaactataca atataaatca tagtacggtt tttacagatt caaaacatgt 720aatgatagtt agatattaag aaaattacaa aatatatttt taaacaaaat agaacactct 780ttattaatca tcaaatggaa tataacataa ttaaatagta tagtaacaaa aaggtagaaa 840acaacaagac acatctggga agaagaactg gccgttgtta cttcatgttg ccgtgtctcc 900tcttccgtca aacaagacac catcgatttc acaatcagag aaacctcccc ttcagccacc 960caccgagaat gcaaagatgt tatactcttg caaaaatcaa ctctgataat cgtgtccgtc 1020tcattgctct ctcccaaatc tgagaatcaa acgatttaat ttctcgaaaa agaagaaagg 1080taaaaattac ggagatgatg acggagcaaa tacggtggcg gcgatgaaac aaacgatgat 1140gatgatggtt gtgctggttg tacatgtaga gatgattgaa gctatgtgac agagatttat 1200gtgttcgtcg tataaaaaaa cagaaatttg agagagagag agagagagag aggaacacga 1260agaaacaggg aagaagaaga aaaaattatg tgtggatgtg tgactttcta ctggacacaa 1320attattttta aaatataatg attattaatt tttttttctg caggttttac cggttacaac 1380agagggcaat acggcaatat tatataaaag acacatgata cagtgaaaaa atagggtttt 1440tagttataga ttgaattata atttttttgg tggttataca gcccaatttc ccttaaaatt 1500ttacatatcg ttgaaagcca gaaaaatatg tggaagtgta ataaaacaat tttctccagg 1560aagaagatat ttcagccatt cgcccatcta tgtgtatgag atcccattca aggccatatt 1620ctaagattga ctttatctaa tttactgtat tttccgtggg ctactgaatg aatacgatgc 1680ttttgttgta aaacgcaacc tattgttctc cattttgaat ttgaagtgtt acattgttga 1740taaaaaggaa gaaagtacaa agcttttcat ttagtggttg ttgcatatat atatacttat 1800ttgatgggtt tatgataagt atgaacatta ttagattatg gttaagtgat ggtttaataa 1860ctggtttagc ttccttttac taaaccggtt gtataaatac atcctcttgt acattgtaaa 1920ctttaacttg agcaatatac tttttccttt tcatcgtaac aaactttctc cattgatctt 1980gagctcaagt tctaatatgg tatcagagcc aatctgatct tcgttcttcc gcttcgttct 2040ttctggattc aagcttctgt agctgcgatt cagtctgatt tcatcttctt tttcgttttc 2100tcacttcttt ttctcgggaa aatcagctcg atcttcttcg cttaagcgca ttcttcatct 2160caatgagtct ctcgttacgg cgcctgatcg atctttttgt tgagctgtat cttcccatcc 2220tttttgcgat ttatcagtcg gtttctatga taatcgcgat tctgtttctt tcttttcttt 2280ctctttgatc ttcgatttgc gtattctctt ctcgcgcaat cgttttctgg ttttccaaat 2340tgattcttgt tttctcatga cgactcctgc atcaacgatc cctctctccg acgataatcc 2400acctgtttcg gcgtcacttc ttgatcccaa ttccaatcct ctgtatgttc acaatgccga 2460tcacgctggt atctctcttg tttctgaaaa gctagctggt ttggggaatt ttaatacttg 2520gcgtagatcg atgatcatgg cactgggagc taggaataag gcagtttttg tggatggtac 2580ttatctggaa ttatctgaaa cgcatccgga ttatgcatct tggtcacgtt gtaacaatat 2640cgtttgcaca tggattgtga atgctgtcga gaaatccatc gccaagagca taatgtacct 2700tcctacggct cgtcaaatgt ggcttgacat ccatgatcag tttaagcaga gtgatggacc 2760aagaacagcc gagataaagc aacaaatttt tgcggaggtg cagggctctc aaactgttag 2820tgaatactac actcgtttga agcaacaatg ggaggagttg aagaatcatg agagtcctta 2880tacttgctgt tgtggacgtt ttgattgttc gagcatgaag aggattgatg agggagagga 2940acaggacaag atcttgaagt ttctcacggg gttgaatgat acattcactg ctactcgggg 3000tcagatactg atgatggatc caaaaccgaa catttctaga gtgtttaatc ttgtttcaca 3060agaggagcga cagaggtcta tgaagagcac aagtgtggta gcgtttcaag tttctcaagg 3120agcatctaat gatgagatgg ttgctgcata tcactctggt tataacaaac agaagcagcg 3180ctcgatatgc tctcactgtg gcattgctgg tcacactgtt aaccgctgct acaagcttca 3240tggttatcca caaggatata gaggtgctac gggttcctcc aagttctcta ctcaggcttc 3300ttcttcatct tcttctgtga agagtcaacc agctggtggg attgatcttt tggaccatca 3360aggtgcgtgc ttggggtctg tcactacagc acaagtcagt cgatttctaa gtgtcttggg 3420ggctcatccg tcgagtctca atgacaatgt ttctcaggct tcagctagca ctattaatca 3480ttctgatgga acagtctcta caccaaaacc acaaccccac ctcgtccccc atttaccgtt 3540acctcaccct tcttcaggta ccactcatta catcaattct cattttgttt ctgctggaac 3600caataatggc cttacctttc taagtgatgc gtggatcata gatactggtg ctagttgtca 3660cgttagttcg aacttaggtt tgtttcattc aactagaacc attagtaaca catctgtcac 3720tcttcctgat ggaacccaaa ttccggtcac aacttctggt tccattactc tctctgaaca 3780tttaactctt cttgatgtct tatacattcc gcattttcaa ttcaatcttt tgagcattag 3840tgctctcact cgcaacagta ctattgctgt tttgttttct tctcattcat gtttcatttt 3900tcctaacaat ccatttctcc tccaggagca tactctggat tgcacgattg ggaagggtaa 3960cctcctgcgc aatctgtatg tcctggagtc tcctacactt tcttcacatt ccgcgcattc 4020tttgtcggtt tctacacctt cttctatgga tcttcctctt ttacaagtct ctgctgatgt 4080gtggcatcaa cgccttggtc atccttctca aaacaagatt caagctcttt caaaactttt 4140gaagatttct tcggctaaga ttgatcatag tagtttgtgt tctgtgtgtc cattagctaa 4200gcaaaagcgt ctgtcgtttc ctttgtctcc tcatttgtcc aaaactcctt tcgatcttat 4260tcatatagat atatggggtc ctttctcgac tactacacat gatggttata gatatttttt 4320aactttggtc gatgattgta ctcgtgctac ttggttatat cttttaaagg ataagagttc 4380ggtttcaaca atttttcctg agtttcttgc ttttgttcat actcaatata agactcatgt 4440taaatccatt cgttctgaca atgccccaga gcttgccttt actgatctgt tgcgtgaaaa 4500aggaattatg cattactttg catgtccata cacacctcaa cagaattctg tagttgaacg 4560aaaacaccaa catattctaa atgtcgctag agctttgctg tttcaatcaa aagtaccatt 4620gatatattgg ggagattgtg ttcaaactgc tgtgtatttg ataaatagga ctccctcacc 4680gcttttgaat gataagtctc catttgaact tctctcaaaa cagattcctt catatgatca 4740tctaaaagtg tttggttgct tatgttactc ttctacacta gctaaagata gaaataaatt 4800tactcctcgt gctgacgcat gtatttttct tggttatccg aatggctata aagggtacaa 4860agttttaaat ctcacgtctc attcgataaa tgtcactaga aatgttgttt ttcatgaaac 4920agttttccca tttcataaat ctactgattc ttctactcat gatgatttct ttgctcagga 4980tgtcttacca ttacctgttc cggattctat gtttcctgct ttctttgact tgggttctga 5040tattgattct tcaatgtttc ctgatccaac tactattagt tcttccaaca tttctaatca 5100ttcgtttgat gcgtctcttc atccttctgt ttctacttct gttccttctt cttcttcttc 5160attaccatca ggaactagaa atgaaactag gactcgtcgt caggttaagg caccagctta 5220tctagatcaa taccattgct atcttttaca aaaagtgcct atggaaccag ttcatcctac 5280tcatacgact tcatacccta tatctgcatt tctttcttat gataaatttg atgaaacaca 5340ccgaaacttc attctttcca taaccaccac caaaccaccc caaacttacc aagaggctgt 5400tctatctgaa gaatttaaga atgctatgaa gtctgaaatg acttctctag aagagacagg 5460gacttggact gtttgtgagt tgcctgcagg taaacacccg gttggttgca aatgggttaa 5520tacttacaag tataaagctg atggtactgt ggaaagaccg aagtctcgtc ttgttgccaa 5580gggctatact cagttggagg gtcttgatta tcttgacact ttttctccgg ttgcgaaact 5640tggtacactt cgtcttctct tagctgttgc agcagctaaa ggatggtcta ttcaacagct 5700ggatataagc aacgcatttc tcaatggtga cttggatgaa gagatatata tgcggcttcc 5760tcagggatat gaagaactca ctggaaagaa gtgtcctcca aactctgttt gtcggctcca 5820taagtctctt tatggcctta aacaggcttc aagacaatgg aatcacaaac tctctagtgt 5880gatcttgggt gatggatttg ttcaaacaca ctctgaccat tctctcttca tcaaacacaa 5940tgagaaggct tttctagcag ttttggtcta tgtcgatgac atattgatcg tctccaacga 6000tgactctgct tctgctacat tcatgaatgt cctcaagtcg gcattcaagt tacgtgatct 6060tggtcaagct aggtacttct taggctttga gattgcacgt aactcctctg gaatttcttt 6120gaatcaacgg aagtattgct tggagctatt ggaagaagct ggaatgctgg gttgcaagcc 6180tgtatctgtt cctatggaac ctaatctgaa gctttcggct actcctggtg acgttttacc 6240ggatgcttct gtttacagga ggctagtggg tcgcttatta tacttgactc acacaagacc 6300tgacatcact tatgctgttc ataagctaag tcagttcatg tctgcgccta cgactgatca 6360tctacaagca gcatacagag ttcttcgcta tctcaagaat gatccagctc agggtctttt 6420ctattctgca acttctgcga tcaaactcac agcttattct gatgctgatt gggcttcatg 6480tccagattct cgtcaatctg tcacaggtta ctgcatgttc ttaggtgact cgttgatatc 6540ttggcgtgca aagaaacaac ctactgtttc ccggagtagc tcagaggcag aatacagagc 6600catggctgat gctacttgtg aacttatctg gttgactgcg ttgttgattg agatgcattg 6660tgctcctgtt gctcctgcaa ccttgttttg tgacaatcag

tcagcgttat acattgcgtc 6720taacccggtc tatcatgaac gcaccaagca tgttgagatc gactgtcaca ttgttcggga 6780gcggttgcag agcggcttca tgaaggctct acacgtccgg tctgagcttc agttagctga 6840catattcact aaggttgttc aaccaggctt gttcaagagt ttagtaagca agattggaat 6900tcatagtcta tgcattccat cttgagggga gggtattaga ttatggttaa gtgatggttt 6960aataactggt ttagcttcct tttactaaac cggttatata aatacatcct cttgtacatt 7020gtaaacttta acttgagcaa tatacttttt ccttttcatc gtaacatact ttccccattg 7080atcttgagct caagttctaa taaacatatt acgtaacggg tttattttgt cgtcaacaca 7140ttatcggcaa tgtgaaaaga aggggtgagg tatctccatt tcaattcttc tctttatata 7200ttaatcgaat tatttacgta tgaaatgaac gtttatatag aaatttcgtg tggaaaacga 7260catgtacacg gcatctcaag accaattagt aatatacttt agtggtgatt acatgtttac 7320ttatccaatt gagaatttaa agcatcgaca ataccttaat gtcgattaag ccgtccccac 7380ttcatgtaat gagttatggg gggagagaga gatcccgaaa ttcgtcaaat aaaacaactt 7440agaactaaaa accgacacca agtatcataa aggaaatgtt gaagaagtca tttatcgtat 7500ccagctcaca attcctaaga ttaaatcatg accgttggaa gagcttataa gattaaactg 7560aagaaattgt gggttttaga agaaagacaa gaaagagaag aacatgatct tacattgcct 7620attttggtgt ataggagttg tcaaaaagag gagagagagg agacaattag gtcaaataaa 7680tgagcactaa aaatggagac atgtgttgag taactattac aagagcgact tatgcttcta 7740tatggcaatg atatcatcac caaagtgcaa tgcccctttt tgccctagtt tcgtaaagtc 7800tctctccttc ttcgtcctta ggaaaaaccc taaattaaat cctgtgttct tgatctttct 7860ttttgagtaa ccatgatttt gaccacacac tagttcttct atattttgtg gtctatagga 7920ttttgcttta tatgtgtttc ttgtattgct ccgtacgtgc gtatataaat ttaaatggtt 7980acaacaaggt ttattataaa taggcacaaa ttagtccatg aagttattta gcttgcacaa 8040gtataatttg ttaagtattt aaatatataa atttgttaca aaacttaatt aaatttatct 8100gattatattt tctttagtgt tcttcctttg ccaacgttga ggtagctatt attattatta 8160ttttgaacat tatgtacgta gttatcttgg ctagttatga ttcgaattct taatttggat 8220cacacttaac agtatttaaa atattcttag aactaaaata attaagagtt acctttaaat 8280tgaagtattc gtgctaaaca gaaactagaa taaacaaatg attgcatgtt aatttttttt 8340ttcgattttc ctatcagaat aaacacatga ttgcatgcaa attttgtttt tgattacgtt 8400atcttttgtt tattttagtt ttgatgctaa ttaatatttt ttattaacaa ctcacataca 8460ttctacctga ttctaggtca gataatgaca cagcgcaaca aaattaatac aaaaccttcg 8520gaaagtagaa taccgcagaa gtaacttttt tgggtacata cgaaatacag tgaaatctct 8580ataaattaat aatgttggga ctataccaaa actataattt tttattaatt tatagagatt 8640aatttatcga tatactaatt gaatcaaaaa cttaatttga gactaaaaaa ttatattatt 8700ttatagagat ttttagtgta tattaattta tagaatatta ttttataaaa aattttagtg 8760tgtattaatt tatagagtat taatttaaag aggttatact gtaatgtgaa tcttcgaaaa 8820acatgccata cataaccacg gatcatagtc gacacctcaa cgtgaagcaa atttgacaat 8880ctacatacat aaccaacaaa aagtagaata ccttgaaaat ctaaaaccca aaatatgatg 8940taaaactcaa gcttggtcca gagcataaaa aaattaaagc catcgctttg gtatcacata 9000tttaaacgtc agtttttttt ttttttttgg gggggggggg ggggggggta atataaaaat 9060ataattaaca aaaaaaaatt atgaaacaat tagcatgtaa aacactaatc ttttggttgt 9120gacaaaacgt tttcacaaat gttctataaa taaattcaag tgcattttat ctgcaaaata 9180tatactttca ctcataaaat aagagcgttt aaaacattca tacacgcact acattgacat 9240gacaaaagaa atccgcaaat acacatgatg tatgtcgaaa aaaacaaaaa atacacatga 9300tgtatatata gagaggatag tatctaggaa ataagactat attatatata taaagaaaat 9360agagaaaaga taaaaatata aattggtatg tataaaagaa aggtctatgc gtctctagtc 9420caaaaactct atatatgtct ggctcaaaag cagatgcagc catagcccca atagtcatga 9480tggagcatca tcatctcctt atgaattgga acaaacctat tgatctcatt acagaagaaa 9540actcttttaa ccacaatcct catttcatag tagatccacc ttccgaaacc ctaagccact 9600tccagccccc gccgacaatc ttctccgatc acggaggagg agaggaagca gaagaagaag 9660aagaagaaga aggagaggaa gagatggatc cgatgaagaa gatgcaatac gcgattgctg 9720ccatgcagcc cgtagacctc gatccagcca ccgttcctaa gccgaaccgc cgtaacgtaa 9780gggtaagcga cgaccctcag acggtggtgg ctcgtcggcg tagagaaagg ataagcgaga 9840agatccggat attgaagagg atggtgccag gcggtgcaaa gatggacact gcctccatgc 9900tcgacgaagc catccgctac accaagttct tgaaacggca ggtgaggcta gcttcttcag 9960cctcacactc agcttggagc tcctatgtct gacccttctt gcctttgtta ttaccacaac 10020tcggatacct aattataatt ctatcacgcg tttcatgttg atatatatag ataaatggtc 10080gaataaggat ttcgatcgaa gattgtatgt acaataaatg atgtgtgtat ttcaattaat 10140gtatgatata tatatatata tgtatgcagt atgcatttat attctattct ctataaggag 10200gcaacattgc cggattaggg ctttgatctt atgcaagttt tccgaccaaa aatatgaaat 10260acttgtttgg atataacata tgaatcggat aagtgttact agttatataa ctggaaaaca 10320aatgtctgga ataagaattc ccgggagaac caagcctttc tctaatccct aagattatag 10380ctactgaaac aatgaaacaa tgaagaatca gttgggcatt agtaaaaaaa aaagaatcag 10440ttgggttgct tataaaattt tgttataaaa tttatgtcgt atgtgtgtta gccgtagctg 10500taaatttatt tcctttgtat gttaattgta aaagtaaatt tattattccc gatgttttta 10560atgatgtaaa tttaaatcaa gaaaacatat taagttctgt taataaatgt attatttttg 10620tttactaatt tccaataatt ttttcaagaa gtacaatttt ttgtttgaaa tttctaattt 10680atcattattg aataatagaa catacctaaa aaccataaaa catgtattcc ttgaaacatt 10740ttttttaata taaaaattat cttttaggag caggtaaagt actttttttt ataaaattgt 10800tagtgtattt atgttcgtta tagatgtaga tattttgcat aataattaat aatatatgag 10860atgtgtaatg tatatatgtg agagaattgc catatttatt aagcggtttt tgtaaaattg 10920actcaaaatt taaagtcaac cacaaaacta acatatgttt ttttggacat ttcatttgcc 10980ctattcactc cacaagttca gattatttac gaaaatgtca ttaatttttt ttctttctga 11040aatggtattt ttactctctc aacctcatca tcttcaagta tttacaagat tgtcattgcc 11100atcaatacac caaccaccat gaacaaccaa tttgaataat gcaccttaaa tcgatttaca 11160cttcttcctt ctcaattctt atgaactaaa aacaacatct ctttcacttt ctctccatat 11220tcatccaaaa aatctaagat ttttattcaa aaatttttat ggttcataga accattgaag 11280cttacgattc ttggtgtgtc acttttgttt gagattatag gtgcttggag aagacttttg 11340tgtgctaaac aagttatctc aatggttgaa ctatgaaaat cagtgttttt tccagatttg 11400ttcgcccaac cgactttcgg gtaagttttc tggctgtaga cgactaacat ggaagtcttc 11460tggtcaatgc acttctcttt gattgtcttc ttctgattta cattatgtag aatgaacaag 11520agatgggagg aactaggggt aggtaaaaaa aacggaaccg aattatcgaa ccgaacagac 11580ccgaaaaaaa ccgaaccaaa tttatgaatt atccaattgg atactgaaat tgtatacccg 11640aagaattaga atcgaactcg taccgaaccg aaaaccgaat aatacccata tagaaccaga 11700tgaaaaacct ttatacacta cttatacgta tatctttata gtttatattt tatacacttg 11760ttttttttat tgatcaacgc ttgttttata ggagaacctt gtattgtttg gatattttat 11820cctcataatt taagttttgg tttctgaatg ggaaaatcat ttgaatgtta ttttggtgtt 11880ggttttaatt catttatttg caagtacatc agattaattt ggataatatg gttacaaagt 11940aaaaagaaaa aactgaactt caggaaccta aaaaaccgag aaccgatctg atctgatccg 12000aaatttttat ggttctttaa tggttaccaa tttcaagaac cgaaagaaac gagaaccgaa 12060agaaccaata accgaagaac cgagcgccca ctcctaggag gaaccaaaca atactttgat 12120ttgaagccca agataattgg aaccatgtta tattacgcag cttatctgat gtgggcttta 12180tgtagaactg ataatgggct ttaaggcagc ctatttcggt ccgtcaagaa gattcctcct 12240aacgtaaggt aggggcaatg agctgctact ctgaccaatt aattacgttt caatagtgat 12300ctctcatctg ttgtcggtgt gaaaggaatc gtcttttgaa tttatcttca tgcgattcat 12360tgttttataa ctaccacaca tgtgacataa ctaatagtct cgaatcagga catcaaaatt 12420tatttttttg tttgtggcaa tgattcaaat cgacggcata gcatactgat taaaaatata 12480tacaatacac tggttattaa aacgttatag ttgtctagat tttaataaca ggtgataata 12540gatcaagtat atagtaaatg ttttatctca cccattacat atttcatatt aaacatttca 12600gatttatttg taaatggatt caactgcctg ttacccttca ctccttttga ttaacatcgc 12660gtcgcaagtc acaaccacca tttagttggg gcgattcgta tattttggga gtatataata 12720acatacataa ctatcaaacc aactggtgtc gacctttggt gttaactaat ttgatttaac 12780tcttttttgg aataaataaa ataaaatttc agagcagctg ttcaatagta aaatgacagg 12840tgggatctag ggtttgtggt ccttacgatc gattgattac tactacttaa cttggcatta 12900tttggagtca cttttgctta ttaataatta gattttgttt acaaacgtgt ggtgatagtc 12960tgggacaagt tgacctgttg acacccaatc atttagaaca ccttttacaa atataaatat 13020tcgcacactg ttacaaacat tatcatcaaa ttattgtgga attttgcaga caaagcatat 13080gccattgtgt ttccttcgat ttggctttta tgcttattcg tggtgtcata attaattcgt 13140gaaaacgaat gattttacat actgtaagta gggctgaaag cggatcgaat agtaccatat 13200tttctactat ttgtgattta ttccatattt cagttttctt cgtaaaaata cgaatatcca 13260gtttttcgaa tatccggaat ttttttgaca tttgcgaata ttcacagata tttacagatg 13320attcatccac tttgttcaat acaagtctaa aaaaatatca aattattttt tagaaatatc 13380ttttacatga taaaaatatt aagtaaaaat taatgaaact aaatgtttta ttaaatttta 13440aaattagtaa tttttcttat aaaaatgttt ttagaaaaat atgtagtttt gtataaagat 13500ttatatattt ttaatttaat attttataag tagtttttaa aataaaattt aataaattat 13560atgttaaata attatataag attatacatt taaaactcta tatttaatcg tagatataca 13620tctatttgta caactccgat ccgtctctaa ttttttttag tatttgtggt ttggttcgtt 13680tttaacggat attgattttt aatatttgct tttttcagag acttacgaat attcatattt 13740ttttggatcg agtcgaaaca aataataaat tgaatcaaat ttaacggata aaatgttcag 13800ccctaactat ggttaactat ggatactgaa accaccactt atcaacaaaa aaaagtagtt 13860gttcgcttct ggtttagagt gaggttacaa attcaaaaca taataattat tttctcggac 13920gataaccaga tcctttttag aggctttaaa aggtaaatca tagtcatgtg tagcctatac 13980atacaaatta aattacaatg gaatatgcat ttattgtcaa tatgtataac acatatgtag 14040acgtaaggta atatatcatt ttggatttga aggaggaagc tgttgctgca tttgctgata 14100gaacgcagcc atcctgctat atgcttccat cgtcgttggc tgcatgtcaa tgcttcatta 14160gatttaaaca aaaatgaagt ataaaagttt tatataaaac aaaaagttgt ataacctgcg 14220aggagcatgc aagaaaggct gacataggat cggggataag aggagactga aatcgagggt 14280cgatggaaga ggaggcatcc catgatgtag aagtcatggg cacaaatggg ttctgcatca 14340tgttggcatg gatattagga gccgttgcag tagcagctcg gttcatagaa ctaaggtcga 14400caagacctag accgggcatc cccatgcgaa gccctaagcc catggaactg gacatgagat 14460ttagttgaag ttgttgttgt tgctgctgca tggccatggg aagcatcatg gagggcatgt 14520tcattctgct catcatgcac acttgtgctt ggagttgctt caagtactct atcacttcat 14580ccaacatgga cgctttatcc gtctagttcc aaattgtaac ttatcaaacc gttcactcct 14640tttgatgtat tatttaacca aaccaactca agggatgatc gatgaacaca tgtctacaat 14700aataatagta cactatacat tttttttttg tcaaacatag tacactatac atatgtaacg 14760tagattgtgt taattgattg tgttggttga attttgattg accgccaaaa ggtggcatcc 14820ctttcttatt atttgaggaa cattagataa aactaacatt tacttcatgt gtttattaca 14880tgtgtgtcat ttcctacgtg gcatcaccac aagctctctc acaccttata ttcaaacacc 14940ctttcttaac tagactaatt acaaaatttg ccattgctaa ttacattaaa acttcaatat 150005714640DNABrassica napus 57attattattt gtatttatat atctagggta ttaaaatctt tttacttatt ttatgaaaca 60ttttggttat ctttctcctt acaatctatt tttgtgataa aaacttgaaa atagtctact 120taggagaatt accctttaca ttatctaata aactgatctt ctagaagaga gaaacgttaa 180tggtggacga agcccccacc tcaattgaca ataaaagaaa gtggaaaaga gagaagatag 240cagagtgaaa ggtccgaaga gaggagatca tgaagagaag aagacaatgt aacctactta 300tttgccttga tgaacaatta cgagaagaaa taaacataaa atgagaagag agaggtggga 360tatacagaag agagaaagat ttggcaggaa acccgattga ccatatatca gatctagagt 420ggattggatt agataactcg agcttatttg gttaatcttt ttagtttagg gaaggttatg 480aaaaattaac cccgatctga tttttaaatt ttttatctta tattgtgccc ccggctaaca 540atatatctag atccgtccct gatcaggtcc atgagatata tcatattttt atttttctta 600tttaattaat tatatatata atatttgagg atatatttaa aattttacat atcgttgaaa 660gccagaaaaa aatgtggaag tgtaataaaa caattttctc caggaagaag atatttcagc 720cattcgccta tttatgtgta tgagatccca ttcaaggaca tactgttttc cgtgagctac 780tgaatgaata cgatgctttt gttgtaaacg caacctattg gttttttttt tccgccaagt 840tttgttttta ttaatcaaac gggccaagcc caaacggtcg aagcccaaat acactaagta 900ccaaacaaaa ggcccacaga cacaacgggc tacaacatta atggctgaag cccaagtaaa 960ctaaacggcc caaaggccca aacattgaaa ccggacagct tgacctaggc gcgcgataag 1020gagactgggg tttgaacacg tgtaagaatc tgggccatta gtatgacacg cgtcgcccgc 1080ctcgacttga tcgaaacctc ttcgcacagc cgcatgaact tgccaccgac gacctcgttt 1140cgccggagct cagagactga agagcctcca acgaaaccat agctattcta cgccgctctg 1200tcttcacgcc ggagagttcc atcgatctaa cgagatctcc tccgactatg tatcaccaca 1260aaccctagac aggcgagccg agaaccactg atatcctcct cttgtacgtc ctcgccgttg 1320tcggagagtt tcatcatcat ccgagatctc atccgcgatg gcccacgacc ccatacctga 1380ccacaagaac cacaccgcac ttatctcacc tcgatctaat caccacaacc caaaggacat 1440cgggttccaa gacggcagcg cggagctttg caagcctcca ctctccgtga ccaaaagccg 1500gcgaagacgg agctgaatga gcctcccctt cccggaagct aaagcggcga cggtggatct 1560gagaaagcct ccacctcccg cataaagacc ggcgacgatg gatctagggt agcctccatc 1620tcccggaaaa aacaccacct gaacatgcaa gccgctccat ctccaccggg agcctccaat 1680tgatcgcgtc gttactccaa aggccgagcc accgttgatg gatggttgcg gtcccagagc 1740tccgacaagg cgatcgatga cgggactgcg aagaaggagc ggagagtgac aactacaaag 1800gaagggaaag agctctggcg acagcacgga cgctcacgcg ccagccgtac gccgaaccca 1860aaagtagatc tggtttctct cttttctctc tctcgcaaaa gaaaacacgc aacctattgt 1920tctccatttt gaatttgaag tgttacatta ttaataaaaa ggaagaaagt acaaagcttt 1980tcatttagtg gttgttgcat atatagtata cttatttgat gggcttatga taagtatgaa 2040catttcacgt aacgggttta ttttgtcgtc aacacattac cggcaatgtg aaaagaaggg 2100gcgaggtatc tccatttcaa ttcttctctt tatagattaa tcgaattatt tacatatgaa 2160atgagcgttt atatagaatt ttcgtgtgga aaacgacatg tacacggcat cgaaagacca 2220attagtaata tactttagtg gtgattacat gtttacttat ccaattgaga atttaaagca 2280tcgacaatac cttaatgtcg attaagccgt ccccacttca tgtaatgagt tatgggggga 2340gagagaggtc ccgaaattcg tcaaataaaa caacttagaa ctaaaaaccg acaccaagta 2400tcataaagga aacgttgaag aagtcattta tcgtatccag ctcacaattc ctaagattaa 2460atcatgaccg ttggaagagc ttataagatt aaattgaaga aattgtgggt tttagaagaa 2520agacaagaaa gagaagaaca tgatcttaca ttgcctattt tggtgtatag gagttgtcaa 2580aaagaggaga gagaggagac aattaggtca aataaatgag cactaaaaat ggagacatgt 2640gttgagtaac tattacaaga gcgacttatg cttctatatg gcaatgatat catcaccaaa 2700gtgcaatgcc cctttttgcc ctagtttcgt aaagtctctt tccttcttca tccttaggaa 2760aaaccctaaa ttaaatcctg tgttcttgat cttgcttttt gagtaaccat gattttgacc 2820acacactagt tcttctatat tttgtggtct ataggatttt gctttatatg tgtttcttgt 2880attgcaccgt acgtgcgtat ataaatttaa atggttacaa caaggtttat tataaacagg 2940cacaaattag tccatgaggt tatttagctt gcacaagagg tatataaatg tgtgtgtgtg 3000tgttaagtat ttaaatatat aaatttgcta caaaacttaa ttaactttat ctgattatat 3060tttctttagt gttcttcatt tgccaacgtt gaggtagcta ttattattat tattattatt 3120attattatta ttattattat tattattatt attattatta ttattattat tattattatt 3180attttgaaca ttatgtacta tgtacgtagt tattttggct agttatgatt caaattctta 3240atttggataa cacttaacag tatttaaaaa aaaaatacta tttaaaatat tcttagtact 3300aaaataatta agatttaact tgaaattgaa gtattcgtgt taaacagaaa ctacaataaa 3360caaatgattg catgttaatt tttttttcga ttatcctata tatcagaata aacacatgat 3420tgcatgcaaa ttttgttttt gattatgtca tcttttgttt attttagttt tgatgctaat 3480taatattttt tttattaaca acttccatac attctacctg attctaggtc agataatgac 3540acagcgcaac aaaattatac aaaactttcg gaaagtagat taccgcggaa gtaacatttt 3600tgggtagaca tacgaagtag tttgaatctt caaaaatcat gccatacata accacggatc 3660atagtcgaca cctcaacgtg aagcaaattt gacaatctac atacataacc aacaaaaagt 3720agaataccgt gaaaacctaa acccaaaata tgatgtaaaa ctcaagcttg gtccagagca 3780taaaaaaatt aaagccatcg ctttggtatc acatatttaa acgtcagttt ttttttgggg 3840aagtaatata aaaatataat taacaagaaa atttatgaaa taattagcat gtaaaacact 3900agtcttttgg ttgtgacaaa acgttttcac aaatgttcta taaataaatt caagcacatt 3960ttatctgcaa aatatatact ttcactcata aaataagagc gtttaaaaca ttcatatacg 4020cactacattg acatgacaaa agaaatccgc aaatacaaac atatttagtt cggatatatc 4080taggaaataa gactatatta tatatataaa gaaattagaa aaaaagaaaa ttggtatgta 4140taaaagaaag gtctatgcgt ctctagtcca aaaactctat atgtctggtt caaaagcaga 4200tgcagcagcc atagccccaa tagtcatgat ggagcctcat catctcctta tgaactggaa 4260caaacctatt gatctcatta cacaagaaaa ctcttttaac cacaatcctc atttcatggt 4320agatccacct tccgaaaccc taagccactt ccagcccccg ccgacagtct tctccgatcc 4380cggaggagga gaggaagcag aagacgaaga aggagaggaa gagatagatg agatgaagga 4440gatgcaatac gcgattgctg ccatgcagcc cgtagacatc gatccagcca ccgttcctaa 4500gccgaaccgc cgtaacgtaa gggtaagcga ggacccccag acggtggtgg ctcgtcggcg 4560tagagaaagg ataagcgaga agatccggat attgaagagg atggtgccag gcggtgcaaa 4620gatggacact gcctccatgc ttgacgaagc catccgctac accaagttct tgaaacggca 4680ggtgaggctt cttcagcctc acactcagct tggggctcct atgtctgacc cttctcgcct 4740ttgttattac cacaactcgg atacctaatt ataattctat cacgcgtttc atgttgatat 4800atatagataa atggttgaat aaggatttcg atcgaagatt gtatggctat tgattacatt 4860atatattgta caataaatga tgtgtgtatt tctattaatg tatatatgat atatatctgt 4920ttgcagtatg catttatatt ctattcttta tagggaggca acatgccgga ttagggcttt 4980gatcgtatgc aagttttccg accaaaaata tgaaatactt gtttggatat aacatatgaa 5040tcggataagt gttactagtt atataactgg aaaaaaattg tttggtataa gaattcccgg 5100gagaaccaag cctttctcta atccctaaga tcatagctac tgaaataatg aaaaaaaaca 5160aaaaaaaaac aatgaagaat cagttgggca ttagtccaaa aaaaaaaaag aatcagttgg 5220attgcttata aaattttgtt ataaaattta tgtcgtatgt gtgttagccg tagctgtaaa 5280tttatttcct ttgtatgtta attgtaaaat ttaatttatt attcccgatg tttttaatga 5340tgtaagttta catcaagaaa acatattaag ttctgttaat aaatgtatta tttttgttta 5400ctaatttcca ataatttttt caagaagtac aattttttgt ttgaaatttc taatttatca 5460acatacctaa aaaccataaa catgtatttt ttgaaacatt tttttaatat aaaaattatt 5520ttttaggagc aggtgaagta tttcttttaa taaaattgtt agtgtattta cgttggttat 5580agttgtagat attttgcata ataattaata atatataaga tgtgtaatgt atatatgtga 5640gagaaatacc atatttacta agagtttttc gcaatattga ctcaaaattc aaagtcaatc 5700acaaaactaa catatgtttc tttttagaca tttcctttgc cttattcact ccacgggttc 5760agattattca cgaaaatgtc attaaatttt ttttcttttc gaaaatggca tttttactct 5820ctcaatttca tcctcttcaa gtatttacaa gatttccatt gccatcaata caccaaccac 5880aatgaacaac caatttgaag ctcttaatgc acctcaaatc gatttacact tcttcttttt 5940caattcttat gaactaaaaa caacatctct ttcaatttct ccccatattc atccaaaaaa 6000cccaagattt ttattctaaa attttaatgg ttcatagagc cattgaagct tacgattctt 6060ggtgggtcac ttttgttcga gattttaggt acttggagaa gacttttgta tgctaaacaa 6120gttatctcat tggctgaaac tatgaaatca gttttttttc cagatctgtt cgcccggacg 6180acttccggat aagtcttctg gctgtagacg acttacatcg aagtattctg gttaatgcag 6240atgttagttt tgcaattgac ttttaagatg cttactcgaa gattagtact cttctgttct 6300tgtaccttat gagcattcca atagtattat atttttcatt ctaaaatata tttcaatgat 6360attttacttt tcactctata atagagtgaa aatgagttta ctttataatc ccctatatat 6420tatttgtgaa acactacaac ttctttttgt agccacatgt catcactagg atgattttta 6480gaattattag agaaataggt tggtccatct aattatataa taagtttttt attaaattaa 6540ccataaattc attattaatg tcatttatta tttccttaaa taaagattac ggaattgcct 6600aatgtgggta aagtatatat gacaattaat gatcttgaat aataaagata tgataaaaaa 6660aatgtatctt ctatcaaatt tgtttaattt aaaactatta aaataatttt taaaaaaaca 6720caataaccat

attataaaaa tttagatttt tctgtatatt tttatatttt gaactttaaa 6780aaatgacgat aaattactaa aactgttaaa agtctcacat tcaaattttg ctatccatgt 6840tttaaaattt ttgttatgac aaaatacaaa tgattacaaa atcatataag taaaagtcta 6900atttaattaa tcattaagat ttaaaatata tatgtatata tatcattcta aattaaacta 6960taaaccatat tgaataaata aatattttag tttcaaaatt ttactttgaa taattttttt 7020tgataaaagt tttgaactaa cattgataaa tgtttttaaa ctatcaatta ccaaaattat 7080tgatcccaca atgaaaattt tgttatcagt aatttaaagt ttttgctatt aaaaatacgc 7140ataataaaac aaaacatatg agtagaaagc atcatttaaa gaaaaataaa aaaataaaat 7200agacattaat attaaaaata tactgtgtat gttaatatca tttaaattta attacatatc 7260ctatcaaaaa aaatttaaaa aaatgtttgg attaataaaa ttgatttata cgttcgcacc 7320aatataatta tatacgtaat agttactgac ttttaattat tcaatatata tttattattt 7380cataatatgt aagaacatat aatacataaa ataattttta tatataatgt ttatctcgcg 7440caaggcgcgg atcttaatct agtctatatt ataagagtta ttcttgggtt catcccctag 7500ggtgaacctc cagccaatag gatttcttta tttcaaatac gatatatttt aaaaaaataa 7560accaaatatt atcaagttat attatgtttt taaaataaaa aagtaaaaaa aaagtagcag 7620ttacagaaaa aagaattaaa aaaaaaaatt taacgtcgtc agcaaaacac taaaccctaa 7680atcataatcc ctaaacccta aatcataaat tctaaaccct tgggtaaacc taaactcttg 7740ggtaaaccta aactcttggg taaaccctaa accattgggt aaactctaaa cccttgggta 7800aaccctaaac ccttggataa atcataaact ctaaataaaa actctaaacc ctaaaactct 7860aaaccataaa tcctaaaccc taaacccttg agtgttttag tgtttagtga ttttgattta 7920gagtttaaga tttatcctag agtttaagat ttatgctagg gtttatggtt taccaaaggg 7980tttagggttt aagatttagg gtttagggat taggatttag agtttaatgt tttgctgatg 8040acgttaaaaa tatttttttt tgtaattact gttttttttt tttttacctt ttaattttat 8100aaagataata taatttgaca atattggtcg gtgaataagt catattataa tgccaacttt 8160tctctttcct aaaggagcct gcgtgtgcat tctataaagc atgggggacc cacttaatgt 8220ctggtttatt ttggtcgaat aggctatagt ttggtttggt cagatttggg ttttctacgt 8280ttggtttgat tattaagggc ctggctggtt caaccgcagc ggttgcggtt gcggctgcgg 8340gagtttgcgg atgcgggtgg ttgcggtttc taacggtttt aagagattta tacgactggt 8400tctgcggtta gaaattggtg cgtttgatac ttatgactgg ttaactacca aacgcaacag 8460cggttaaata ataaattaac aaaatttaca ttttatatca ttataaaata tcaaaaataa 8520taatattata ataaatataa aatttatatt tagaaagtta tagtttaaac tttttttaaa 8580ttatagaaaa tattttcatt ttaaagtttt ataatattaa ttaaaatata atagatatat 8640tttagtattt ttataattct aatttaaatt ttttattgaa tatttttatt tttgtattta 8700aattgtttta gaaaaaaaaa aatttatcct cccgcaaccg cccgcaaccg caaacgctag 8760ttggaaccag tttttgaatt tatgaggttt agagcgattt ggagcggttt ggagcggttt 8820gagcgattgt tgcaaaacgc caacaaccgc taccaaccgc aaaagctgcg tttgcgggtg 8880gtagcgggaa aaccagtctt acccttgtca ttagggcttc attagttgtt agcgttcttc 8940cacacttcga gtgtgattgg taacatcaga gtaaagtgga gtaaaaaaaa gtggtgaaaa 9000agaggaaaat aaatggtgga aaaatgtgag agagaaaaga atctggaata acatgagtgt 9060acctaaagct agaggaaaaa taggtgtata tttccctcat aatcaatagt gcctagagta 9120aatatgaaaa gatagtattt cattttattg gtgacttttt ggcttaactg agagtaaata 9180tatatctaga gtaatagttt actctataga taccatacaa tcataccctt catcctcgcc 9240gtgaatctgc aaacctaatg acttctcctc ccatctcttt accttccctt ccgttcaata 9300tatttctttt aattcagcaa tagttgttat tcacattaaa cccagtaagg atgtcgacct 9360cttcgttttc ttcacgtcaa cctataaata ggagaaggtt gagagtagga tggatcttca 9420gaaccactgt ctgttaatct tctttgtcac tctcactcac tctctctctc atctgcaact 9480ggtttggccc tcccttcgag ttatgtggac cttgtggttt gtctttctcc agtgagaaac 9540ttggtgagca aacttaatat tattcatctt atgcattact tattgaactg gaagtatctt 9600atacataata cccgtctcaa gaacctatca aagaggcggg aattttggag ggtcgcaatg 9660gtataaagga gacatgccgt caggtatgct taccgtggaa gtcatgttgc aacagttaac 9720aatcatgatt atgtttttaa atttaacttt taaatttgct ttatttctgt gatcacattg 9780gtgcttttat gtttgttttt aggggatagt ctaattattt tcatatatgg acccatcttt 9840cttaagtatg ctggttgctc atgtcgtgtt gcatattagg gacagcgccc taagaacccc 9900ccatgttaga taattctaat taacttaaag agcaagttaa ctcattaaag tgaagtttga 9960tgggttaagg aaaaaggaaa acatatcgag cttaggaatg tttccatatt attattagga 10020aaagatgtag cttcgccttt aggaaaagat gtagtttcgc ctttaggaaa agatgtagct 10080tcttcctata taaagagttc ttatggagag atgttctata aaaaaaaaca cattgaaagg 10140tttagttttg agagagtttc taaatctaat aagaagtgaa gttcttataa tctttgtgta 10200tatgcaactt taaattggta tcagagctcc aggtttcgaa ggtcgtttac aagaggagtt 10260gtaacttcga tcaaaacatg ggagacgatt ctcaagcacg tgataaaggt cttgggatga 10320agaaggacat acgatgtccg atgctaacat cgaccaacta caccgtatgg tcgatgcgaa 10380tgaaagtgat gcttcgttta tacgaagttt gggatacgat tgatccaggg agtaacgatg 10440caaagaagaa taatatggcg attgctctaa tctttcaatc agtcccggag gcgctaatac 10500ttcagattgg agaacatgat acatcgaaga agatttggga agctatcaaa tcccgtaacc 10560taggtgctga tcgcgtgaga gaagcaaggt tacaaacttt gatgtctgag ttcgacaaac 10620tgaagatgac agacacagac acggtggatg actacgcaga aaaactttca ggcttagcat 10680cgagagcagc cgcattagga gagataatgg aagcatcaag gctggtaaag aagtttctga 10740agggacttcc aagaacgaag ttcattcaca tcgtagcttc gttagaacaa gtgctggatc 10800taaattctac gggattcgaa gacattgttg ggagattgaa ggctttcgaa gaacgcatca 10860aagaagaaac ccaagatgaa ggtcaatcga agctcatgtt tggaaagaat gatatgcaag 10920gtagtggaag ccataacaac tcgcgaggaa gaggcatagg tagaggttat ggtagaagag 10980gaagaggaca aggaaggtct aatgtttctg atggtcataa caaaggagga gaagcttcag 11040acaagaccaa gaaggactac tctaaggtta gatgttggcg atgcgacaag atggggcact 11100tcgtgtcaca ttgtcctaca cgaccaagag aagaagctaa cctaacggaa acacaagaag 11160cggatgcatt atatatgcat gaagtagtat tcctcaacga agagagagtg tttcctaaga 11220ggtttgatga atgcgatgga aatacgagta tatggtacct tgacaatggt gcaagtaacc 11280atatgacagg caagagagag ttcttctcaa acctagatga tagcatcaaa ggcaaagtca 11340aattcggtga tggatcaaac atcaagattg ttgggaaagg ttcgatcacg ttcaccggaa 11400aaacagggga gagaagagca ctcaaagata tctactacat cccaagtctt aaacacaata 11460tcataagtct tggacaagca accgagatgg gttgtgaggt taatatgaaa gaagacttac 11520tgatgctgaa agatccccgt ggaaggttat tagtacaagt cgcaaggcaa ccaaaccgat 11580tgtacaagac gccaatggag gttgaatatc cgaagtgtct tcaaatacaa gaaactgatg 11640ttacatggac gtggcatgca cggctaggac atgtaaactt tggagtcatg aagaatatgg 11700tagacaaaga gatggtagta gggatgcctc aggtgataca cgagaaagat gtgtgcaacg 11760cctgcttagt tgggaagcaa actcggaagt ctttcctgcc taaagcaaag tatcgagcat 11820cacacgcatt ggatttggta catggtgact tgtacggtcc gatatcacca tcaacaccag 11880caaacaatag atatgtatac gtcttaattg atgattactc aagatatatg tggacgatgc 11940tgctaagaga gaagagtgaa gcgttcgatc ggttcaaaaa gttcaaggag tacgtggaga 12000atcaaacgaa gctacaactc aagaattttc gcaccgatag aggaggagag ttcacatctt 12060ctgatttcat tcgtttttgt gaagaaaacg gtgtaattag acatctcacc gcatcatata 12120cactatcaag aaacgtggac gaagaaccag gatcattcaa gcttcctcat attgatgtta 12180cagaaggaga tggtgatgag catcaacatc accaacacca cgaagagcaa aacaataatg 12240aagaagatgt gaacttgttt gtaacgggga cttcgctcag tgttatcaag caattcaaag 12300atgatatgtc aagaatattt gagatgtcag acctcgagat gcttacatac tatcttggta 12360tagaagtaac acaaggagct gatggcattc acatcaaaca agaaggatac gcgcaaggta 12420tacttgtcaa gacgaagatg gagtcatgta actatactca cgttctgatg cacacgagtt 12480tgaaagtatc aaaggcagag gaggagcctg agattgatgc aacatcgtat cgaagcacca 12540taggatgctt aagagagagt ataaatgctt cactgcttct ctatgactct ctcttaagca 12600tcttatggtg cttcgatacg atgttgcatc aatctcaggc tcctcctctg cctttgatac 12660tttcaaactc gtgtgcatca gaacgtgagt atagttacat gactccatct tcgtcttgac 12720aattatacct tgcgcgtatc cttcttgttt gatgtgaatg ccatcagctc cttgtgttac 12780ttctatacca agatagtatg taagcatctc gaggtctgac atctcaaata ttcttgacat 12840atcatctttg aattgcttga taacactgag cgaagtccct gttacaaaca agtcatcgat 12900gtatatagct atgatcagaa gctccccctt ctgtttcttt tgatataccg caggttccgt 12960ggtgcacttc gtgaactcca tctccttgag aacacggtcg agtttgatgt tccaagctct 13020tagagcaact ggtgcaaata attcgtcgaa gtctatgcct tgttgttgca cgtagccttt 13080tgcgactagc cttgctttgt atttgatcac cgttccatct acattcctct tgatcttata 13140gatacacttc aaacctatca gtttcacacc tgccggcttc ttgacgagct tccaggtctt 13200gtttttgatg atagatttga tctctgcctt cattgcatcg atccaagctt gtattactgc 13260agcttcgatg taactttctg gttcaccatc gatggtgagc aagagtctgc cactttcaac 13320ttcagcaagt aggatgtaat catcgaaccg ctttggtttt ctgatgttac gaccatatct 13380tgatgttaca tgctggtctt ggtttgcatc gttgttctct gctacttgct cttgttcacc 13440tgcgtctaca gcttcttctt cttcattatt gttttactct tcgttctgat gcaacatcgt 13500atcgaagaaa tgcgatgcac ctagtaacgg tctatggaag ttcatcggag ttcagaagat 13560caacatacct aagattcaag ttggaattaa gggggagaat gttggataat tccaattaac 13620ttaaggagca agttaactca ttaaagtgaa gtttgatggg ttaaggaaaa aggaaaacat 13680atcgagctta ggaatgtttc catattatta ttaggaaaag atgtagtttc gcctttagga 13740aaagatgtag tttcgccttt aggaaaagat gtagcttctt cctatataaa gagttcttat 13800ggagagatgt tccatcaaga aaaacacatt gaaaggttta gttttgagag agtttctaaa 13860tctaataaga agtgaagttc ttataatctt tgtgtttatg caactttaaa ttatatctct 13920tatttaagag acggttctta acttttctta gttaaagcta agaaccgtct cttagctgaa 13980gctaagaacc cagtctctta tctggggtta atcatggtcg tctaatgtct catttattag 14040tgtttttatt tttcacagtc gttaggtact ctcatcatgc tctctcctat ataattcgtt 14100aatgcatgcg cagataccaa ttatcatgct tgttgtggaa gtcccttgtg atctggtatg 14160cagtatacag tcaccaaaag atggcccaga ctgtatcacc gttgaccaag acagcaatag 14220taacactgat gtggttggat ttgaagactc tgcagcagca aactctttac cgacaaaggc 14280tggaaacttg gcaaatgcaa aatgtgtccg tcttgacatc agtttcaaaa cgccatcgca 14340tacgtgtctt caaaccacac aattggtgtt tgtttaattc ttctttcaca gcaagatatt 14400tgtttttctt acattatacc tcttcttgtc tatgtatctc tgtaaccttt tttctttctt 14460tgggcttatg tttcctaaaa tgttcgtttc tgcaggtgaa agatctgaca gcaatttcca 14520gctgccacac cacttgcttt ggtgttaaag cagtttttgg ctgatcgtac cctggatcaa 14580tcatactccg gcggattgag ctcttattgc ctggtgagcc gtgttccttt acattaagta 14640584081DNABrassica napus 58ttagctgttg cagcagctaa aggatggtct attcaacagc tggatataag caacgcattt 60ctcaatggtg acttggatga agagatatat atgcggcttc ctcagggata tgaagaactc 120actggaaaga agtgtcctcc aaactctgtt tgtcggctcc ataagtctct ttatggcctt 180aaacaggctt caagacaatg gaatcacaaa ctctctagtg tgatcttggg tgatggattt 240gttcaaacac actctgacca ttctctcttc atcaaacaca atgagaaggc ttttctagca 300gttttggtct atgtcgatga catattgatc gtctccaacg atgactctgc ttctgctaca 360ttcatgaatg tcctcaagtc ggcattcaag ttacgtgatc ttggtcaagc taggtacttc 420ttaggctttg agattgcacg taactcctct ggaatttctt tgaatcaacg gaagtattgc 480ttggagctat tggaagaagc tggaatgctg ggttgcaagc ctgtatctgt tcctatggaa 540cctaatctga agctttcggc tactcctggt gacgttttac cggatgcttc tgtttacagg 600aggctagtgg gtcgcttatt atacttgact cacacaagac ctgacatcac ttatgctgtt 660cataagctaa gtcagttcat gtctgcgcct acgactgatc atctacaagc agcatacaga 720gttcttcgct atctcaagaa tgatccagct cagggtcttt tctattctgc aacttctgcg 780atcaaactca cagcttattc tgatgctgat tgggcttcat gtccagattc tcgtcaatct 840gtcacaggtt actgcatgtt cttaggtgac tcgttgatat cttggcgtgc aaagaaacaa 900cctactgttt cccggagtag ctcagaggca gaatacagag ccatggctga tgctacttgt 960gaacttatct ggttgactgc gttgttgatt gagatgcatt gtgctcctgt tgctcctgca 1020accttgtttt gtgacaatca gtcagcgtta tacattgcgt ctaacccggt ctatcatgaa 1080cgcaccaagc atgttgagat cgactgtcac attgttcggg agcggttgca gagcggcttc 1140atgaaggctc tacacgtccg gtctgagctt cagttagctg acatattcac taaggttgtt 1200caaccaggct tgttcaagag tttagtaagc aagattggaa ttcatagtct atgcattcca 1260tcttgagggg agggtattag attatggtta agtgatggtt taataactgg tttagcttcc 1320ttttactaaa ccggttatat aaatacatcc tcttgtacat tgtaaacttt aacttgagca 1380atatactttt tccttttcat cgtaacatac tttccccatt gatcttgagc tcaagttcta 1440ataaacatat tacgtaacgg gtttattttg tcgtcaacac attatcggca atgtgaaaag 1500aaggggtgag gtatctccat ttcaattctt ctctttatat attaatcgaa ttatttacgt 1560atgaaatgaa cgtttatata gaaatttcgt gtggaaaacg acatgtacac ggcatctcaa 1620gaccaattag taatatactt tagtggtgat tacatgttta cttatccaat tgagaattta 1680aagcatcgac aataccttaa tgtcgattaa gccgtcccca cttcatgtaa tgagttatgg 1740ggggagagag agatcccgaa attcgtcaaa taaaacaact tagaactaaa aaccgacacc 1800aagtatcata aaggaaatgt tgaagaagtc atttatcgta tccagctcac aattcctaag 1860attaaatcat gaccgttgga agagcttata agattaaact gaagaaattg tgggttttag 1920aagaaagaca agaaagagaa gaacatgatc ttacattgcc tattttggtg tataggagtt 1980gtcaaaaaga ggagagagag gagacaatta ggtcaaataa atgagcacta aaaatggaga 2040catgtgttga gtaactatta caagagcgac ttatgcttct gaaccaagcc tttctctaat 2100ccctaagatt atagctactg aaacaatgaa acaatgaaga atcagttggg cattagtaaa 2160aaaaaaagaa tcagttgggt tgcttataaa attttgttat aaaatttatg tcgtatgtgt 2220gttagccgta gctgtaaatt tatttccttt gtatgttaat tgtaaaagta aatttattat 2280tcccgatgtt tttaatgatg taaatttaaa tcaagaaaac atattaagtt ctgttaataa 2340atgtattatt tttgtttact aatttccaat aattttttca agaagtacaa ttttttgttt 2400gaaatttcta atttatcatt attgaataat agaacatacc taaaaaccat aaaacatgta 2460ttccttgaaa catttttttt aatataaaaa ttatctttta ggagcaggta aagtactttt 2520ttttataaaa ttgttagtgt atttatgttc gttatagatg tagatatttt gcataataat 2580taataatata tgagatgtgt aatgtatata tgtgagagaa ttgccatatt tattaagcgg 2640tttttgtaaa attgactcaa aatttaaagt caaccacaaa actaacatat gtttttttgg 2700acatttcatt tgccctattc actccacaag ttcagattat ttacgaaaat gtcattaatt 2760ttttttcttt ctgaaatggt atttttactc tctcaacctc atcatcttca agtatttaca 2820agattgtcat tgccatcaat acaccaacca ccatgaacaa ccaatttgaa taatgcacct 2880taaatcgatt tacacttctt ccttctcaat tcttatgaac taaaaacaac atctctttca 2940ctttctctcc atattcatcc aaaaaatcta agatttttat tcaaaaattt ttatggttca 3000tagaaccatt gaagcttacg attcttggtg tgtcactttt gtttgagatt ataggtgctt 3060ggagaagact tttgtgtgct aaacaagtta tctcaatggt tgaactatga aaatcagtgt 3120tttttccaga tttgttcgcc caaccgactt tcgggtaagt tttctggctg tagacgacta 3180acatggaagt cttctggtca atgcacttct ctttgattgt cttcttctga tttacattat 3240gtagaatgaa caagagatgg gaggaactag gggtaggtaa aaaaaacgga accgaattat 3300cgaaccgaac agacccgaaa aaaaccgaac caaatttatg aattatccaa ttggatactg 3360aaattgtata cccgaagaat tagaatcgaa ctcgtaccga accgaaaacc gaataatacc 3420catatagaac cagatgaaaa acctttatac actacttata cgtatatctt tatagtttat 3480attttataca cttgtttttt ttattgatca acgcttgttt tataggagaa ccttgtattg 3540tttggatatt ttatcctcat aatttaagtt ttggtttctg aatgggaaaa tcatttgaat 3600gttattttgg tgttggtttt aattcattta tttgcaagta catcagatta atttggataa 3660tatggttaca aagtaaaaag aaaaaactga acttcaggaa cctaaaaaac cgagaaccga 3720tctgatctga tccgaaattt ttatggttct ttaatggtta ccaatttcaa gaaccgaaag 3780aaacgagaac cgaaagaacc aataaccgaa gaaccgagcg cccactccta ggaggaacca 3840aacaatactt tgatttgaag cccaagataa ttggaaccat gttatattac gcagcttatc 3900tgatgtgggc tttatgtaga actgataatg ggctttaagg cagcctattt cggtccgtca 3960agaagattcc tcctaacgta aggtaggggc aatgagctgc tactctgacc aattaattac 4020gtttcaatag tgatctctca tctgttgtcg gtgtgaaagg aatcgtcttt tgaatttatc 4080t 4081593781DNABrassica napus 59agaggagatc atgaagagaa gaagacaatg taacctactt atttgccttg atgaacaatt 60acgagaagaa ataaacataa aatgagaaga gagaggtggg atatacagaa gagagaaaga 120tttggcagga aacccgattg accatatatc agatctagag tggattggat tagataactc 180gagcttattt ggttaatctt tttagtttag ggaaggttat gaaaaattaa ccccgatctg 240atttttaaat tttttatctt atattgtgcc cccggctaac aatatatcta gatccgtccc 300tgatcaggtc catgagatat atcatatttt tatttttctt atttaattaa ttatatatat 360aatatttgag gatatattta aaattttaca tatcgttgaa agccagaaaa aaatgtggaa 420gtgtaataaa acaattttct ccaggaagaa gatatttcag ccattcgcct atttatgtgt 480atgagatccc attcaaggac atactgtttt ccgtgagcta ctgaatgaat acgatgcttt 540tgttgtaaac gcaacctatt ggtttttttt ttccgccaag ttttgttttt attaatcaaa 600cgggccaagc ccaaacggtc gaagcccaaa tacactaagt accaaacaaa aggcccacag 660acacaacggg ctacaacatt aatggctgaa gcccaagtaa actaaacggc ccaaaggccc 720aaacattgaa accggacagc ttgacctagg cgcgcgataa ggagactggg gtttgaacac 780gtgtaagaat ctgggccatt agtatgacac gcgtcgcccg cctcgacttg atcgaaacct 840cttcgcacag ccgcatgaac ttgccaccga cgacctcgtt tcgccggagc tcagagactg 900aagagcctcc aacgaaacca tagctattct acgccgctct gtcttcacgc cggagagttc 960catcgatcta acgagatctc ctccgactat gtatcaccac aaaccctaga caggcgagcc 1020gagaaccact gatatcctcc tcttgtacgt cctcgccgtt gtcggagagt ttcatcatca 1080tccgagatct catccgcgat ggcccacgac cccatacctg accacaagaa ccacaccgca 1140cttatctcac ctcgatctaa tcaccacaac ccaaaggaca tcgggttcca agacggcagc 1200gcggagcttt gcaagcctcc actctccgtg accaaaagcc ggcgaagacg gagctgaatg 1260agcctcccct tcccggaagc taaagcggcg acggtggatc tgagaaagcc tccacctccc 1320gcataaagac cggcgacgat ggatctaggg tagcctccat ctcccggaaa aaacaccacc 1380tgaacatgca agccgctcca tctccaccgg gagcctccaa ttgatcgcgt cgttactcca 1440aaggccgagc caccgttgat ggatggttgc ggtcccagag ctccgacaag gcgatcgatg 1500acgggactgc gaagaaggag cggagagtga caactacaaa ggaagggaaa gagctctggc 1560gacagcacgg acgctcacgc gccagccgta cgccgaaccc aaaagtagat ctggtttctc 1620tcttttctct ctctcgcaaa agaaaacacg caacctattg ttctccattt tgaatttgaa 1680gtgttacatt attaataaaa aggaagaaag tacaaagctt ttcatttagt ggttgttgca 1740tatatagtat acttatttga tgggcttatg ataagtatga acatttcacg taacgggttt 1800attttgtcgt caacacatta ccggcaatgt gaaaagaagg ggcgaggtat ctccatttca 1860attcttctct ttatagatta atcgaattat ttacatatga aatgagcgtt tatatagaat 1920tttcgtgtgg aaaacgacat gtacacggca tcgaaagacc aattagtaat atactttagt 1980ggtgattaca tgtttactta tccaattgag aatttaaagc atcgacaata ccttaatgtc 2040gattaagccg tccccacttc atgtaatgag ttatgggggg agagagaggt cccgaaattc 2100gtcaaataaa acaacttaga actaaaaacc gacaccaagt atcataaagg aaacgttgaa 2160gaagtcattt atcgtatcca gctcacaatt cctaagatta aatcatgacc gttggaagag 2220cttataagat taaattgaag aaattgtggg ttttagaaga aagacaagaa agagaagaac 2280atgatcttac attgcctatt ttggtgtata ggagttgtca aaaagaggag agagaggaga 2340caattaggtc aaataaatga gcactaaaaa tggagacatg tgttgagtaa ctattacaag 2400agcgacttat gcttctagaa ccaagccttt ctctaatccc taagatcata gctactgaaa 2460taatgaaaaa aaacaaaaaa aaaacaatga agaatcagtt gggcattagt ccaaaaaaaa 2520aaaagaatca gttggattgc ttataaaatt ttgttataaa atttatgtcg tatgtgtgtt 2580agccgtagct gtaaatttat ttcctttgta tgttaattgt aaaatttaat ttattattcc 2640cgatgttttt aatgatgtaa gtttacatca agaaaacata ttaagttctg ttaataaatg 2700tattattttt gtttactaat ttccaataat tttttcaaga agtacaattt tttgtttgaa 2760atttctaatt tatcaacata cctaaaaacc ataaacatgt attttttgaa acattttttt 2820aatataaaaa ttatttttta ggagcaggtg aagtatttct tttaataaaa ttgttagtgt 2880atttacgttg gttatagttg tagatatttt gcataataat taataatata taagatgtgt 2940aatgtatata

tgtgagagaa ataccatatt tactaagagt ttttcgcaat attgactcaa 3000aattcaaagt caatcacaaa actaacatat gtttcttttt agacatttcc tttgccttat 3060tcactccacg ggttcagatt attcacgaaa atgtcattaa attttttttc ttttcgaaaa 3120tggcattttt actctctcaa tttcatcctc ttcaagtatt tacaagattt ccattgccat 3180caatacacca accacaatga acaaccaatt tgaagctctt aatgcacctc aaatcgattt 3240acacttcttc tttttcaatt cttatgaact aaaaacaaca tctctttcaa tttctcccca 3300tattcatcca aaaaacccaa gatttttatt ctaaaatttt aatggttcat agagccattg 3360aagcttacga ttcttggtgg gtcacttttg ttcgagattt taggtacttg gagaagactt 3420ttgtatgcta aacaagttat ctcattggct gaaactatga aatcagtttt ttttccagat 3480ctgttcgccc ggacgacttc cggataagtc ttctggctgt agacgactta catcgaagta 3540ttctggttaa tgcagatgtt agttttgcaa ttgactttta agatgcttac tcgaagatta 3600gtactcttct gttcttgtac cttatgagca ttccaatagt attatatttt tcattctaaa 3660atatatttca atgatatttt acttttcact ctataataga gtgaaaatga gtttacttta 3720taatccccta tatattattt gtgaaacact acaacttctt tttgtagcca catgtcatca 3780c 3781606656DNABrassica napus 60aaaaatattt gatttatata ttcttattaa aaatattaat ttactgttta ttattttata 60taattttata aatcatttta tttagagttt aatttattta cgatttattg ttttaatttg 120ttaggcaaat tagtctaaca aattaaacaa aaaaaaatat aaataaatta aattttaaac 180aaaatgtttt ataaaaatat ttatatcctt ataaggatat aaactataat tttgtgttat 240ttttatattt aaactattat tatatttaat tatttttaaa taaatttatt tatatttttg 300tatattttta ttaaaactaa tattttattt aattatgaaa attgaaaacg tttttttggt 360tatttttgaa aattttgaca aggttgaaaa atcgctggaa atatttttga ggaattttag 420acaaaaaaat atcgatgata aacaattggt tggaatccgt caagaatttt tgacaaaata 480aactcgaaat ttgttttcac tgggaattag ttattttctt gtatttgcat tatctaataa 540acaatgcaac cataaacaac aaatgaaata aagttttaat tgacaaatct tttagaagag 600tgaaacgtta atggtggacg aagcccccac ctcaattgac aataaaaaga gtggaaaaga 660gggaagatgg cagagagaaa agtccaaaga gaggaaatca tgaagagaag aagacaatgt 720aacctactta tttgcctcga tgaagataga ggagaagata gaggtggaat atatatagaa 780gagagaaaga tttgggagga aacccgattg agcatatatc agatctagaa tggattggat 840tagataactc gaacttattt ggttaatctt tttagtttag ggaaggttat gaaaaattac 900ctctgatcta atttttaaat tttttatctt atatacattg tgccccccga ctaacaatat 960atctagatcc atccctgatt aggtccatga gatatatcat attttttatt tttcttattt 1020aattaattat ctataatatt tgttgatata tttaaaattt tacatatcgt tgaaagccag 1080aaaaatatgt ggaagtgtaa taaaacaatt ttctccagga agaagatatt tcagccattc 1140gcccatctat gtgtatgaga tcccattcaa ggccatattc taagattgac tttatctaat 1200ttactgtatt ttccgtgggc tactgaatga atacgatgct tttgttgtaa aacgcaacct 1260attgttctcc attttgaatt tgaagtgtta cattgttgat aaaaaggaag aaagtacaaa 1320gcttttcatt tagtggttgt tgcatatata tacttatttg atgggtttat gataagtatg 1380aacatttcac gtaacgggtt tattttgtcg tcaacacatt atcggcaatg tgaaaagaag 1440gggtgaggta tctccatttc aattcttctc tttatatatt aatcgaatta tttacgtatg 1500aaatgaacgt ttatatagaa atttcgtgtg gaaaacgaca tgtacacggc atctcaagac 1560caattagtaa tatactttag tggtgattac atgtttactt atccaattga gaatttaaag 1620catcgacaat accttaatgt cgattaagcc gtccccactt catgtaatga gttatggggg 1680gagagagaga tcccgaaatt cgtcaaataa aacaacttag aactaaaaac cgacaccaag 1740tatcataaag gaaatgttga agaagtcatt tatcgtatcc agctcacaat tcctaagatt 1800aaatcatgac cgttggaaga gcttataaga ttaaactgaa gaaattgtgg gttttagaag 1860aaagacaaga aagagaagaa catgatctta cattgcctat tttggtgtat aggagttgtc 1920aaaaagagga gagagaggag acaattaggt caaataaatg agcactaaaa atggagacat 1980gtgttgagta actattacaa gagcgactta tgcttctata tggcaatgat atcatcacca 2040aagtgcaatg cccctttttg ccctagtttc gtaaagtctc tctccttctt cgtccttagg 2100aaaaacccta aattaaatcc tgtgttcttg atctttcttt ttgagtaacc atgattttga 2160ccacacacta gttcttctat attttgtggt ctataggatt ttgctttata tgtgtttctt 2220gtattgctcc gtacgtgcgt atataaattt aaatggttac aacaaggttt attataaata 2280ggcacaaatt agtccatgaa gttatttagc ttgcacaagt ataatttgtt aagtatttaa 2340atatataaat ttgttacaaa acttaattaa atttatctga ttatattttc tttagtgttc 2400ttcctttgcc aacgttgagg tagctattat tattattatt ttgaacatta tgtacgtagt 2460tatcttggct agttatgatt cgaattctta atttggatca cacttaacag tatttaaaat 2520attcttagaa ctaaaataat taagagttac ctttaaattg aagtattcgt gctaaacaga 2580aactagaata aacaaatgat tgcatgttaa tttttttttt cgattttcct atcagaataa 2640acacatgatt gcatgcaaat attgtttttg attacgttat cttttgttta ttttagtttt 2700gatgctaatt aatatttttt attaacaact cacatacatt ctacctgatt ctaggtcaga 2760taatgacaca gcgcaacaaa attaatacaa aaccttcgga aagtagaata ccgcagaagt 2820aacttttttg ggtacatacg aaatacagtg aaatctctat aaattaataa tgttgagact 2880ataccaaaac tataaaattt ttattaattt atagagatta atttatcgat atactaattg 2940aatcaaaaac ttaatttgag attaaaaaat tatattattt tatagagatt tttagtgtat 3000attaatttat agaatattat tttataaaaa attttagtgt gtattaattt atagagtatt 3060aatttaaaga agttatactg taatgtgaat cttcgaaaaa catgccatac ataaccacgg 3120atcatagtcg acacctcaac gtgaagcaaa tttgacaatc tacatacata accaacaaaa 3180agtagaatac cttgaaaatc taaaacccaa aatatgatgt aaaactcaag cttggtccag 3240agcataaaaa aattaaagcc atcgctttgg tatcacatat ttaaacgtca gttttttttt 3300tttttttggg gggggggggg gggggtaata taaaaatata attaacaaaa acaatttatg 3360aaacaattag catgtaaaac actaatcttt tggttgtgac aaaacgtttt cacaaatgtt 3420ctataaataa attcaagtgc attttatctg caaaatatat actttcactc ataaaataag 3480agcgtttaaa acattcatac acgcactaca ttgacatgac aaaagaaatc cgcaaataca 3540catgatgtat gtcgaaaaaa acaaaaaaat acacatgatg tatatataga gaggatagta 3600tctaggaaat aagactatat tatatatata aagaaaatag agaaaagata aaaatataaa 3660ttggtatgta taaaataaag gtctatgcgt ctctagtcca aaaactctat atatgtctgg 3720ctcaaaagca gatgcagcca tagccccaat agtcatgatg gagcatcatc atctccttat 3780gaattggaac aaacctattg atctcattac agaagaaaac tcttttaacc acaatcctca 3840tttcatagta gatccacctt ccgaaaccct aagccacttc cagcccccgc cgacaatctt 3900ctccgatcac ggaggaggag aggaagcaga agaagaagaa ggagaggaag agatggatcc 3960gatgaagaag atgcaatacg cgattgctgc catgcagccc gtagacctcg atccagccac 4020cgttcctaag ccgaaccgcc gtaacgtaag ggtaagcgac gaccctcaga cggtggtggc 4080tcgtcggcgt agagaaagga taagcgagaa gatccggata ttgaagagga tggtgccagg 4140cggtgcaaag atggacactg cctccatgct cgacgaagcc atccgctaca ccaagttctt 4200gaaacggcag gtgaggctag cttcttcagc ctcacactca gcttggagct cctatgtctg 4260acccttcttg cctttgttat taattaccac aactcggata actaattata attctatcac 4320gcgtttcatg ttgatatata tagataaatg gtcgaataag gatttcgatc gaagattgta 4380tggctattga ttacattata tattgtacaa taaatgatgt gtgtatttca attaatgtat 4440gatatatata tatatgtatg cagtatgcat ttatattcta ttctctataa ggaggcaaca 4500ttgccggatt agggctttga tcttatgcaa gttttccgac caaaaatatg aaatacttgt 4560ttggatataa catatgaatc ggataagtgt tactagttat ataactggaa aacaaatgtc 4620tggaataaga attcccggga gaaccaagcc tttctctaat ccctaagatt atagctactg 4680aaacaatgaa acaatgaaga atcagttggg cattagtaaa aaaaaaagaa tcagttgggt 4740tgcttataaa attttgttat aaaatttatg tcgtatgtgt gttagccgta gctgtaaatt 4800tatttccttt gtatgttaat tgtaaaagta aatttattat tcccgatgtt tttaatgatg 4860taaatttaaa tcaagaaaac atattaagtt ctgttaataa atgtattatt tttgtttact 4920aatttccaat aattttttca agaagtacaa ttttttgttt gaaatttcta atttatcatt 4980attgaataat agaacatacc taaaaaccat aaaacatgta ttccttgaaa catttttttt 5040aatataaaaa ttatctttta ggagcaggta aagtactttt ttttataaaa ttgttagtgt 5100atttatgttc gttatagatg tagatatttt gcataataat taataatata tgagatgtgt 5160aatgtatata tgtgagagaa ttgccatatt tattaagcgg tttttgtaaa attgactcaa 5220aatttaaagt caaccacaaa actaacatat gtttttttgg acatttcatt tgccatattc 5280actccacaag ttcagattat ttacgaaaat gtcattaatt ttttttcttt ctgaaatggt 5340atttttactc tctcaacctc atcatcttca agtatttaca agattgtcat tgccatcaat 5400acaccaacca ccatgaacaa ccaatttgaa taatgcacct taaatcgatt tacacttctt 5460ccttctcaat tcttatgaac taaaaacaac atctctttca ctttctctcc atattcatcc 5520aaaaaatcta agatttttat tcaaaaaaat ttatggttca tagaaccatt gaagcttacg 5580attcttggtg tgtcactttt gtttgagatt ataggtgctt ggagaagact tttgtgtgct 5640aaacaagtta tctcaatggt tgaactatga aaatcagtgt ttttttccag atttgttcgc 5700ccaaccgact ttcgggtaag ttttctggct gtagacgact aacatggaag tcttctggtc 5760aatgcacttc tctttgattg tcttcttctg atttacatta tgtagaatga acaagagatg 5820ggaggaacta ggggtaggta aaaaaaccgg aatcgaatta tcaaaccgaa caaacccgaa 5880aaaaccgaac caaatttatg aattatccaa ttggatactg aaattgtata cccgaagaat 5940tagaaccgaa ctcgtaccga accgaaaacc gaataatacc catatagaac cagatgaaaa 6000acctttatac actacttata cgtatatctt tatagtttat attttataca cttgtttttt 6060ttattgatca acgcttgttt tataggagaa ccttgtattg tttggatatt ttatcctcat 6120aatttaagtt ttggtttctg aatgggaaaa tcatttgaat gttattttgg tgttggtttt 6180aattcattta tttgcaagta catcagatta atttggataa tatggttaca aagtaaaaag 6240aaaaaactga acttcaggaa cctaaaaaac cgagaaccga tctgatctga tccgaaattt 6300ttatggttct ttaatggtta ccaatttcaa gaaccgaaat aaacgagaac cgaaagaacc 6360aataaccgaa gaaccgagcg cccactccta ggaggaacca aacaatactt tgatttgaag 6420cccaagataa ttggaaccat gttatattac gcagcttatc tgatgtgggc tttatgtaga 6480actgataatg ggctttaagg cagcctattt cggtccgtca agaagattcc tcctaacgta 6540aggtaggggc aatgagctgc tactctgacc aattaattac gtttcaatag tgatctctca 6600tctgttgtcg gtgtgaaagg aatcgtcttt tgaatttatc ttcatgcgat tcattg 6656618882DNABrassica napus 61gtgaaatttt gtgttattca cttaaattat aatatctatg tataccaaag agaagaagat 60aacatatatt atagtatttg gtacatacta tactataaca tacttagtaa atttcacacc 120acaacaaaaa taatataaaa ggtcacattt taatatacaa cgtgccaaac ttcgtccatt 180tgtaatctta tacattttcg tcttcgcata tgatagatca taagatttgg agaaacatat 240aaaattaacc tatgaaatta agggggtcta tatcttatag tatgtatcca aagaacatat 300ataggaaata tttgaataca tcataagatt tggggaatat aaattaatat aaaataaaat 360cttccaattt taaacgtaaa cgtctcctct gtatttatat aaacttttca aaatcacggt 420gtcgctcgtg ttaagatttg gatttctaat ccaactttca gaaagtatat gtgaaagata 480aaactcaaac acaatagttc gtttagaacg ctcttaagat taatgtcaca cacacaaaat 540tgatgtcact agatttattt gttttgtcgt ttaaggggat ccatcaacta cacatggaag 600aatagaaaac actggtcaca cacaaaaaat aatataagca tgttgaattc tattcatcaa 660agaattttct tcaaaggatg aacaataacc tataattagg aaacattata acaacgagca 720ggaagttttc ccagaagtca tgccttcgtc cataccttcg ggtctttcaa gcaagcttca 780cacatgcctt atcatatgtt tgtatacgtt ttcattttgt gtacgtctat taactagcta 840gtatatgcca agattatata ttttatatag tcgacaatta cctatcctaa aagttcattt 900taatttttat tcagaaagat atgcacgtag ggccactagt gtttcccgtt gttattggaa 960attcatttcc atgtgttgaa ttgtttgttt aagtttttga acccgtaagt caaatttatt 1020ttggtttaac agggtttcat tactgttata agcttgtcaa gaaagaattt gttacaaatt 1080tcgaggctca tgcatgcatg attcttaact ttctagaaac atgaaatgtg gttttaccag 1140ctcaagcttg tgttaaaaca catttttaat ttgaccacgt gtgatattca aatatcaaca 1200attctgttca acgggagatt catacatatg ttcagatgtt ctttaatctt aagcacctaa 1260ctttgcttcc catacgcaaa tgtgtgtgcc gcgaactatg gtgaaatgtt ttctttttgg 1320agcattttta aaagaaaata taatttactt atttccgttt tgattgatat acatgcatta 1380ataaaccgac ttacgtgggt gatgggatga gcaaaattaa aatatgcatt ttagtaacca 1440attacggtct atcttgacaa actaacaaac aaaaagtcta ttatgacgtt gatttgtgac 1500ctggccatat aaatgccaaa atttcattct ttcaataagc aacgagatca ataagatgtg 1560gcagtcacgt gaacgttagt tatcgccatt tcaactgaac cttcacgaac atataaagtt 1620catttgttct ttgctaaaat tacgcaaatc tctgttgtgc acgaaattac gactattttt 1680taaatacaac tcctgttctt gtatcgtgta tctctcgcac gtaaatttga attatggggg 1740cattatcgat aaaatatgta aagaatatat tttcatcata caccacaaac aaatgttctg 1800gaaatgtcac cttttatcac atatacaaag ctgaccctga aattttggaa actacaagcc 1860aaaaaaaatg gtcaaaagaa atattatgca caaaggttgg actcgtccta actcttttta 1920caatgataat taacctttga acaactaatt aaagatacat acaatatgtt ttttttctaa 1980aaattggtcg attaaatatt catatataat ccttatcatc atttattagc cttttgtagc 2040ttacttaaaa aaccccttcc caccaaattg attagtgtag attgattatt tttcacttca 2100tacgttgatc aattttaaaa tatatatata tatatatata tatatatata tgcacgtccg 2160gcgtcccatg acttcagacg cttttctgtt tccttgattt tataggttaa aattttccaa 2220aatgctcata tttttcactc atatgtataa tgtattatta tacgcacaaa atagtaaagt 2280cataaacatt tatgatggct cttttatatg tatagagtag tgaaatttat atatatcgga 2340gttccgctta aaataaatta atattgttct tttatcgccc attttttagt tactaaacgt 2400atataattaa aaactgaaat gcaatatgta gaaatccggc ttatgatgaa ccatactaat 2460actcattgtt acaaactcac agcaacatca taattccatc actcgccaca ataattttct 2520ttagtgtatg ttctatttca cattttgacc cttcactttc ttgaatgaaa attatacaag 2580aaaatgattt gagtgtgtta aaaaaaaaat gttttgagtc attctgacca attatctggc 2640agagactata ataaaatact tccaattacc ttaaatcatt aggcgttgtt tcttgccgaa 2700tatatgatgg agcatggtct cgctaagaga caacaaaatc tttaaataaa ttaaacaagt 2760tgtttagcaa aaaataaaaa ataaattaaa caagagagag tacaaatata aaactctaaa 2820ggaagataaa tgtataaaat acaaaatata gaatagaggc cttaagtagt gtttacgtga 2880caatctcaat ttcactccat acctcgaact cattcaagct tagaaaccta acttttactg 2940ttccttttga tttgcctata aatacacgtt catccatact tctgtaattt caaataacaa 3000agcaaaacac aaagataaga atcacaaaca acaaacattc tcaacctaat cccaaaaaga 3060cgacacgcat tcctttttcc aaaaatccac ttcctacgta ccaaccacaa tggctcattg 3120ttggacagtt cttaccggtt tactagtatg gtctcttttg atgctctcat ggtgcgaagc 3180ttcaagaaac aacattaata gatatgatca ttcttatgga acgttcaaat ccaatagctt 3240aatcaagcga agagacgata tcactcgtct gaaaagtgtt gcaagagctt ctttgcggct 3300tccaaccacc gttagtgttt ctgattttgg agctaaagga gatggaaaaa ccgacgacac 3360gcaggtatat acgcatacac acatatctac atgacttata agttataaat attgtgagac 3420ggtcacgtat ataaatttac gttgtttgta ctaatgatcc atgcatgcgc atgcatatga 3480tcaggcgttc gtgaatgcat ggaagaaagc ttgttcttca agtggacctg taaatctctt 3540agttcctgaa ggaaaaactt attttctcaa gtctcttcga ttaaatggcc catgcaaatc 3600agttcttacc gttcaggtaa attacaaagt ttcttttgtt ttgcttaaat ttgggtaaat 3660tacaaagttg acaaaaaaat tacaaagttg actatttgtt aattatatac gtatactaca 3720tgtgatcacg tatatggttt tgaatatatt aaagagcggt tggtgaatgg aacgacagat 3780tttaggaacg ttatcggcat ctcaacaacg atcggattat aaagatctca gcaaatggat 3840tacgtttgac ggcgttaaca gtctaacggt tgatggcggg gccaccggaa ctgtcaacgg 3900aaacggcgaa acgtggtggg aaaactcatg caaacggaac gaagctaagg tgtgtgtctg 3960tacatgtttc atttacttaa tatatatcta ctatctacat gtcatcttga tatattgaca 4020cttttttatt gttattgttt tgaagaaatg cacaaaagcc ccaacggtac gtagaaacat 4080atttgacatt ttcatgtaat actgacgatg gctgtgatca tgattggaca aatgttttac 4140atgcatgaat atatgtattt tatatgcagg ctcttacttt ctacaactcg aagaatttga 4200gagttaataa tctgagggtg aaagatgcgc aacagatcca gatttcgatt gagaaatgct 4260ccaacgttca ggtctcaaat gttgaggtca ctgcgcctgc tgatagtcct aacactgatg 4320gtatccacat cactaacact caaaacattc aagtctccaa atccatcatt ggaacaggtt 4380agtaattaca tcctcgttat ttaaaagagt tgcgtgcata tccagaaaac taatatttaa 4440gggtttatcg ttataattta tagagattgg tcttaaaaga gttttttaat gtttgctttt 4500ctatgcaggt gatgattgta tatctattga aagtggatca caaaacgtta aaatcaacga 4560tttaacttgc ggccccggtc acggtatcag gtatcaatga attgttttca gtaaatatac 4620ctaagaaata caaccgtaat cttttacata taaatttgaa atttccttat aaaacatgag 4680cagcattggg agcttaggag atgacaattc aaaggcattt gtatcaggcg tgactgtgga 4740tggtgctaaa ctttccggta cagacaatgg agttaggatc aaaacttacc aggtgaaaat 4800cacaaactaa accttcaatt ttaactagcc tatcaagttc atcccaattt aacttttaaa 4860cccaattcaa tatagggagg gtctggaact gctagcaaca taatatttca gaacattcag 4920atggagaatg tgaagaatcc aatcataatc gaccaagatt attgcgacaa gagcaaatgc 4980accgaacaag ttcgtttgaa cctaatcaaa tcttccatta ctaatcctca tgataataaa 5040cacttttatc tctaatcatt tactacatat attttcagaa atcagcggtc caaatcaaga 5100acgtggtgta ccggaacata agcggcacaa gcgcatcaga catagcaata actttcaact 5160gtagcaagaa ctatccatgc caaggaattg tactcgacaa agtgaacatt aagggaggga 5220aagcgagttg cagcaacgct aatgtgatgg ataaagacgg tgttttgcct cagtgcaaat 5280ccacttgatt aactagacct atcaaaaact cacacagatg tataaataca tatagaccga 5340tacaaagtat acaaaattat gatatatact tgtggatata tacatggaca atgaatttaa 5400acatattatt tatatgtcaa aatagtttat gaatagacat ataaacaaca tgttcaagag 5460atttattgtc ttaatgtata tactccatac taaattgttt gtctaaaact tcatataata 5520aaaaactgtt aatcttcatg atggtgtgat ttgttgtgat aacagtatga tgtctattgt 5580agatttttat ttaaatacat aaaaattgaa agaaaaaaaa tgacatggct acttgaccaa 5640ttgattcaag ttaattgtta ctaaccaggc cgataaacta attgggcctt taaagcccat 5700taatatttgt cagtggaagc gaagtctgtt acttgggtta caaaacaaag gcctttagaa 5760aaccctaact cttatcgcag cacctgagct atatattgcc gcagctaggg ttttccctag 5820ctcacgctaa cacacaaagc cgcagccatt tccatctctc tcgcaactca acttcgaaca 5880atggctgaac gaggcggaga acgaggcgcc gagcgaggag gagaccgcgg cggtttcgga 5940cggggattcg gaggaggtcg cggcgaccga ggaggccgcg gaggtccgag aggccgaggc 6000ggcagacgcg gaggacgtcc cacagaggag gagaagtgga ccccagtgac caagctcggc 6060cgtctcgtgc aagccggaaa gatccagaag ctcgagcaga tctacctcca ctcgctcccc 6120gtcaaggagt accagatcat cgacctcctc gtcggtccaa ctttaaaaga cgaggtgatg 6180aagatcatgc cggttcagaa acagaccagg gccggtcaga ggacgaggtt caaggctttt 6240gtcgttgtcg gagacacgaa tggtcatgtt ggtttgggtg tgaagtgctc caaggaggtg 6300gcgacggcga tcagaggtgg gattatattg gcgaagctgt cggtgattcc cgtgaggaga 6360gggtactggg ggaacaagat tgggaagccg catacggttc cttgtaaggt tacggggaag 6420tgtgggtctg ttacggtgag gatggtgcct gctccgaggg gggcggggat tgtggcggct 6480agggttccta agaaggtgct tcagttcgct gggattgatg atgtgtttac ttcttccagg 6540ggatctacta agactcttgg aaacttcgta aaggtttgaa ctttattcat ttacttggtg 6600tttggagctg tgtatagatt gaaagttgat tcctttttga tcgttgcgtg tggaggtgtt 6660ttgggtaata ttgctaaatg taaaaattgc taagcttgat tagtttgaat aagctgtctg 6720tgttaaatga tgatttgttt gtgttgtaat gagctgtctg aatttcatta tttgtttgtg 6780ttattgctaa tttacaagct taaatttgat tccttttgat cgtttcgtgt gagttgtttt 6840gtgtaattta gcttgatgta gttgatggaa gtaagaattg ttaagcttta gagctgtctg 6900ttaatgttga attgtttgtg ttgtattgag ctgtctgaat tattatttgt ttgcgattta 6960tttctaaatc acaaagctct aaagtcgttt gaacatatgc atcatttggt tagtgtttat 7020tgctgaattt tcatgatttg gttgtgttta ttgctaaatt tgagattaca ttataatctt 7080aatttgtaca tatattcatc atttttgtgt ttattgattt gtttgtgttt attgatgaat 7140cacaagtttc tatgattaat cttcatgatt tggttgtgat tattgctaaa tttgagatta 7200cattataagc ttagtttgta catgtataca tcatttcact tgaattttca cagacgagct 7260ggtgatatat tttttttttt agtattttcc atgtattgct ttgtaagaac gtttaaatgt 7320tagttttctt gaatgttttt ttctttgtaa gaacaattaa atgtttgttc agtatttttg 7380aatcattcat tggttaatca tgttcaacat gtattttttg taggctacat ttgactgtct 7440ccagaagact

tatggattcc tgacaccaga gttctggaaa gagacgagct ttaaaaagtc 7500accgtaccag gagtacactg atttgctggc cgagaaggga actgcaacca aagccatcac 7560cgaggttgaa gaccaagccg cctcctaaat aaacaaacaa gcaatgtcca ccttgttttg 7620tttttggatg atcctttcat gagttttatt ttgttcgaac cttttagtat atgttgtttg 7680ctggattgct gctatcttaa gttttcatca tcttcccaaa caaagttatt tttgctctag 7740tttttttttt tttaataatc gatggttata ctctagtatt tttttacttg aatttctcta 7800attttctatc agttgcgcta tgttacacat tctaaggttg attacatagt tgatccgaaa 7860tgattaaaaa ggctggatgt tgtaataaat tgttggatat acttttagaa taatataatt 7920agggggatta aattataaat taaaattaaa aaagacaagt cttaaatgga ttgttgaact 7980gtgagaaggt gaaaaggtgg agactttgaa tagaagagaa taaatcaaaa gttgacgtat 8040cttctccgaa atttcagctg tttctaaata cgcggattct gattactctg ttctctcttg 8100cctgataaac cttcgaattg agtttttttt ttttttttca tttctttttt tggttcttgt 8160cggaatcgtg agaagctctg tctctttgtc ggaaccaatg gggactccgg aatttccaga 8220tctggggaaa cactgctccg tcgatatctg caagcagatc gatttcttgc ctttcacctg 8280cgaccgttgt ctccaggttc ctctttcctt ctctctctgt tttgaatcac atgcaaaatg 8340aatcgtcttt gttgcgaata attgtgttgt atcactgtct gaatcgcatg tcttcgatcc 8400tgggaactac tatatcatct tccttgttct tttgtcgttc gtgttcttgg agaaccaaga 8460gaaaagtccc cgtctttctc ttattaccca agaggaaaat catcttttct acatgcgacc 8520agtttgatat aggatcgagt ttgatttgat ttgttccgac attaagtttg tcacttaagt 8580tctttgtaat tacgaccttg ttctttggca tttcgcaatt ctccgcactt aattcaccag 8640agttcttcaa aattcactta actcatcatg attgccatta aagattcgat ctttttgttg 8700gtttacatta acatatttga gtttatggct ggttcttatg ttttcgctag aaatttgcat 8760ttaaagattt gttcttttta tgttaatgac ttgtgtgttc gctcttgttt acattgattt 8820gatctttttc aggtcttttg tttggatcat cggagctata tgaaacacac ttgccccaaa 8880gg 8882625668DNABrassica napus 62tggtgttaag atttgattta tggatgcaca tagttggata cattttatgt ttaaaatgtg 60tttttaatat tattattgct gtggtgtgaa attttgtgta ttcactttag ttataatatc 120tatgtatacc aaagagaaga agataacata tattatagta ttggtacata ctataatata 180acatacttaa ttagtaaatt tcacaccaca gcaaaaataa tataaaaggt cactttttaa 240tatacaacgt gccaaacttc gtccatttat aatcttatat atttttgtct tcgcatatga 300tagatcataa gatttggaga aacatataaa attaacctat gaaattaagg ggatctatat 360attatagtat gtatccaaag aacatatata tgaaatattt gaatacatca taagatttgg 420ggaatataaa ttaatataaa ataaaatcgt ccaattttaa acgtaaacgt ctccgctgta 480tttatataaa cttttcaaaa tcacggtgtc gctcgtgtta agatttggat ttctaatcca 540actttcagaa agtttatgtg aaagataata ctcaaacaca atagttcgtt tagaacgctc 600ttaagattaa tgtcacacac acaaaattga tgtcactaga tttatatgtt ttgtcgttta 660aggggatcca tcaactacac atggaagaat agaaaacact ggtcacacac aaaaaataat 720ataagcatgt tgagtcctat tcatcagaaa attttctcca aaggatgaac aataacctac 780aattaaggaa acttattata acaacgacca agaagttttc ccataagtca tgctttcgtc 840cataccttcg ggtctttcaa gcaagcttca cacatgcctt atcgtatatt tgtatacgtt 900ttcattttgt gtacgtctat taattagtat atgcctagat tatatattta tatagtcgac 960aattacctat cctaaaggtt tattttaatt tttattcaga aagatatgca cgtagggcca 1020ctagtgtttc ccgttgttat tggaaattcc atgtgttgaa ctgtttgatt aagtttttga 1080acccgtaagt caaatttatt taggtttaac agggtttcat tactgttata agcttgtcaa 1140gaaagaattt gtcacaaatt tcgagactca tgcatgcatg catgtttctt aactttcgag 1200aaacatgaag tgtggtttta ccagctgaag tttgtgttaa aacacatttt taatttgacc 1260acgtgtgata ttcaaatatc aacaattctg ttcaacggga gattcataca tatgttcaga 1320tgttctttaa tcttgagcac ctaactttgc ttcccatacg caaatgtgtg tgccgcgaac 1380tatggtgaat gtacggcatc gtttttcttt ttggagcatt tttaaaagaa aatataattt 1440agttatttcc gttttgattg atatagatgc attaataaac cgacttacgt gggtgatggg 1500atgagcaaaa ttaaaatatg cattttagta accaattacg gtccatcttg acaaactaac 1560aaacaaaaag tctattatga cgttgatttg tgaccttgcc atataaatgc caaaatttca 1620ttctttcaat aagcaacgag atcaataaga tgtggcagtc acgtgaacgt tagttatcgc 1680catttcaact gaaccttcac gaacatataa agttcatttg ttctttgcta aaattacgca 1740aatctctgtt gtgcacgaaa ttacgactat tttttaatga caactcctgt tcttgtatcg 1800tgtatctagc acgtaaattt gaattatggc ggcattatcg ataaaatatg taaagaatat 1860attttcatca tacaccacaa acaaatgttc tggaaatatc accttttatc acatatacaa 1920agctgaccct gaatttttgg aaactacaag ccaaaaaaaa atggacaaaa gaaaaattat 1980gcacaaaagt ggactcgtcc taactctttt tataatgata attaaccttt gaacaactaa 2040ttaatacata caatatgttt tttttttcta aaaattggtc gattaaatat tcatatataa 2100tcctcatcat catttattag ccttttgtag cttacttaaa aaatcacttc ccaccaaatt 2160gattattttt tcacttcata cgttgatcaa tttaaaaaaa aaataataat aatatatgca 2220cgtccggcgt cccatgactt cagacgcttt tctgtttcct tgattttata ggttagaaat 2280ttccaaaatg ctcatatttt tcactgatat gtattatacg cacaaaatag taaagtcata 2340aaaatttatg atggttcttt tatatgtata gagtagtgaa aatttatata tattgtagtt 2400ccgcttaaaa taaattaata tttttctttt atcgcccata tttaagttac taaacgtata 2460taattaaaaa ctgaaatgca atatgtagaa atccgggcat tgattgttga ttaacattga 2520tatacccatg cgactggctt atgatgaacc atacctgcta atactcattg ttacaaactc 2580gcagcaacat cataaatcca tcactcgcca caataatttt cttcagtgta tgttcaattg 2640atcacatttt gacccttcac tttcttgaat gagaaattat acaagaaagt gatttgagtg 2700tgttaaaaaa tgatttgtca ttctgaccaa ttatctggca gagagtataa taaaatactt 2760ctaattacct taaatcatta ggcgttgttt cttgccgaat atagctaggg tttccactcc 2820ctcacgctaa cacacatcac cgcagccatt tccatctctc aaccaacttc aaacaatggc 2880tgaacgaggc ggagaacgag gtgccgagcg aggaggagac cgcggcggtt tcggacgcgg 2940attcggagga ggtcgcggcg acagaggagg ccggggaggt ccgagaggcc gaggcggcag 3000acgcggagga cgtccgacag aggaggagaa gtggacccca gtgaccaagc tcggccgtct 3060cgtgcaagcc ggaaagatcc agaagctcga gcagatctac ctccactccc tccccgtcaa 3120ggagtaccag atcatcgacc tcctcgtcgg ccccaccttg aaagacgagg tgatgaagat 3180catgccggtt cagaaacaga ccagggccgg tcagaggacg aggttcaagg cctttgtcgt 3240cgtcggagac acgaacgggc acgttggttt gggtgtgaag tgctccaagg aggtggcgac 3300ggcgatcaga ggtgggatta tattggcgaa gctgtcggtt attcccgtga ggagagggta 3360ctgggggaac aagattggga agccgcatac ggttccttgt aaggttacgg ggaagtgtgg 3420gtctgttacg gtgaggatgg tgcctgctcc gaggggtgcg gggattgtgg cggctagggt 3480tcctaagaag gtgcttcagt tcgctgggat tgatgatgtg tttacttcgt cgagggggtc 3540tactaagact cttggaaact tcgtaaaggt ttgaactttc ttcatttatt tagagtttgg 3600agttgtgtat ggattgaaag ttgattcctt tttgatggtt gcgtgtggag ttgttttggg 3660taatttagct tgttgtagtt gatgtaaagt gagaattgct aagcttgatt agtttgaata 3720agctgtctgt gttaaatgat gatttgtttg tgttgtattg agctgtctga atttcattat 3780ttgtttgtgt tattgctaaa ttacaagctt aaatttgata acttttgatt ctttcgtgag 3840agtttttttt gggtaatata gcttgatgta gttgatggaa gtaaattgtt aacctttaga 3900gctgtctgtt aatggtgatt tgtttgtgtt gtattgagct gtctgaatta taatttgttt 3960gtgtttattg ctaaatcaca aagctttgaa gtcgtttgta catattcatc atttttgtgt 4020ttattgactt gtttgtgttt attgctaaat cacaagtttc tatgattaat cttcatgatt 4080tggttgtgtt tattactaaa tcacaagttt ctatgattaa tcttaatgat ttggttgtgt 4140ttattgctaa atttgagatt acattataat cttagtttat acatgtataa catcatttca 4200cttgaatttt cacagacgag ctggtgatat gtttttttag tattttccat gtattgcttt 4260gtaagaactt ttaaatgttt gttttcttcc tttgaagaac aattaaatgt ttgttcagta 4320tttttgaatc attcattggt taatcatgtg taattttatt tttgtaggct acattcgact 4380gtctccagaa gacttatgga ttcctgacac cagagttctg gaaagagacg agctttaaaa 4440agtcaccgta ccaggagtac actgatctgc tggccgagaa gggaattgca accaaagcca 4500tcaccgaggt tgaagaccaa gccgcctcct aaataaacaa acaaacaatg tcccccttgt 4560tttgtttttg gatgatcctt tcttgagttt tattttgttc gaacctttag tatatgttgt 4620ttgctggatt gctgctttct tatgttttca tcatctcccc caaacaaagt tatttttgct 4680ctagtttttt tttataatcg atggttatgc cctctagtat tttttttact tgaatttttc 4740actaattttc tatcaacttc cctatgttac actttctaag gttgattaca taggtggatt 4800gttgtgaaac aaatactaat aataaactaa tacttttaga ataatataat tagtgggatt 4860aaattataaa ttataaatta aaaagacaag tctgaaatgg attgtttaac tgtaaaaggt 4920aaagagactt tgaatagaag agaataaatc aaaagttgac gtatcttctc cgaaatttca 4980gctatttcta aatacgcgga ttctgattac tctgttctct ctcgcctgat aaaccttaga 5040attgagtttt tttttttttt atctttttgg ttctttttta gttcttgtcg gaatcgtgag 5100aagctctgtc tctctgtcgg aaccaatggg gactccggag tttccagatc tggggaaaca 5160ctgctccgtc gatatctgca agcaaatcga tttcttgcct ttcacctgcg accgttgtct 5220ccaggttcct ctctctctat ctctgttttg aatcacatgc aaaaaagaat cgtctttgtt 5280gcgaatagtt gtgtcgtatg actgtctgaa tcgcctgtct tcgatcctgg gaactactat 5340atcatcatcc ttgttctttt gtcgttcgtg ttctgggaga accaagtgaa aagtctccgt 5400ctttctctta attaccgcga ggaaaattca tcttttctac atgcgaccag tttgatttga 5460tttgttccga cattaagttt atcacttaag ttcttgtaat tacgaaccaa cgtgttgagc 5520catctgtcat ttcgagctta atcatcgaca tttcgcaatt ctccgcactt aaattcacca 5580gagttgttca aaattcactt aattcatcat gattgccatt aaagattcga tctttttgtt 5640ggtttacatt aacatatttg agtttatg 5668635717DNABrassica napus 63catatatgga tggacatagt tggacatatt taggtcctta tccaaatgat gttttaagat 60ttgatttatg gatgcacata gttggataca ttttatgttt aaaatgtgtt tttaatatta 120ttattgctgt ggtgtgaaat tttgtgtatt cactttagtt ataatatcta tgtataccaa 180agagaagaag ataacatata ttatagtatt ggtacatact ataatataac atacttaatt 240agtaaatttc acaccacagc aaaaataata taaaaggtca ctttttaata tacaacgtgc 300caaacttcgt ccatttataa tcttatatat ttttgtcttc gcatatgata gatcataaga 360tttggagaaa catataaaat taacctatga aattaagggg atctatatat tatagtatgt 420atccaaagaa catatatatg aaatatttga atacatcata agatttgggg aatataaatt 480aatataaaat aaaatcgtcc aattttaaac gtaaacgtct ccgctgtatt tatataaact 540tttcaaaatc acggtgtcgc tcgtgttaag atttggattt ctaatccaac tttcagaaag 600tttatgtgaa agataatact caaacacaat agttcgttta gaacgctctt aagattaatg 660tcacacacac aaaattgatg tcactagatt tatatgtttt gtcgtttaag gggatccatc 720aactacacat ggaagaatag aaaacactgg tcacacacaa aaaataatat aagcatgttg 780agtcctattc atcagaaaat tttctccaaa ggatgaacaa taacctacaa ttaaggaaac 840ttattataac aacgaccaag aagttttccc ataagtcatg ctttcgtcca taccttcggg 900tctttcaagc aagcttcaca catgccttat cgtatatttg tatacgtttt cattttgtgt 960acgtctatta attagtatat gcctagatta tatatttata tagtcgacaa ttacctatcc 1020taaaggttta ttttaatttt tattcagaaa gatatgcacg tagggccact agtgtttccc 1080gttgttattg gaaattccat gtgttgaact gtttgattaa gtttttgaac ccgtaagtca 1140aatttattta ggtttaacag ggtttcatta ctgttataag cttgtcaaga aagaatttgt 1200cacaaatttc gagactcatg catgcatgca tgtttcttaa ctttcgagaa acatgaagtg 1260tggttttacc agctgaagtt tgtgttaaaa cacattttta atttgaccac gtgtgatatt 1320caaatatcaa caattctgtt caacgggaga ttcatacata tgttcagatg ttctttaatc 1380ttgagcacct aactttgctt cccatacgca aatgtgtgtg ccgcgaacta tggtgaatgt 1440acggcatcgt ttttcttttt ggagcatttt taaaagaaaa tataatttag ttatttccgt 1500tttgattgat atagatgcat taataaaccg acttacgtgg gtgatgggat gagcaaaatt 1560aaaatatgca ttttagtaac caattacggt ccatcttgac aaactaacaa acaaaaagtc 1620tattatgacg ttgatttgtg accttgccat ataaatgcca aaatttcatt ctttcaataa 1680gcaacgagat caataagatg tggcagtcac gtgaacgtta gttatcgcca tttcaactga 1740accttcacga acatataaag ttcatttgtt ctttgctaaa attacgcaaa tctctgttgt 1800gcacgaaatt acgactattt tttaatgaca actcctgttc ttgtatcgtg tatctagcac 1860gtaaatttga attatggcgg cattatcgat aaaatatgta aagaatatat tttcatcata 1920caccacaaac aaatgttctg gaaatatcac cttttatcac atatacaaag ctgaccctga 1980atttttggaa actacaagcc aaaaaaaaat ggacaaaaga aaaattatgc acaaaagtgg 2040actcgtccta actcttttta taatgataat taacctttga acaactaatt aatacataca 2100atatgttttt tttttctaaa aattggtcga ttaaatattc atatataatc ctcatcatca 2160tttattagcc ttttgtagct tacttaaaaa atcacttccc accaaattga ttattttttc 2220acttcatacg ttgatcaatt taaaaaaaaa ataataataa tatatgcacg tccggcgtcc 2280catgacttca gacgcttttc tgtttccttg attttatagg ttagaaattt ccaaaatgct 2340catatttttc actgatatgt attatacgca caaaatagta aagtcataaa aatttatgat 2400ggttctttta tatgtataga gtagtgaaaa tttatatata ttgtagttcc gcttaaaata 2460aattaatatt tttcttttat cgcccatatt taagttacta aacgtatata attaaaaact 2520gaaatgcaat atgtagaaat ccgggcattg attgttgatt aacattgata tacccatgcg 2580actggcttat gatgaaccat acctgctaat actcattgtt acaaactcgc agcaacatca 2640taaatccatc actcgccaca ataattttct tcagtgtatg ttcaattgat cacattttga 2700cccttcactt tcttgaatga gaaattatac aagaaagtga tttgagtgtg ttaaaaaatg 2760atttgtcatt ctgaccaatt atctggcaga gagtataata aaatacttct aattacctta 2820aatcattagg cgttgtttct tgccgaatat aagctagggt ttccactccc tcacgctaac 2880acacatcacc gcagccattt ccatctctca accaacttca aacaatggct gaacgaggcg 2940gagaacgagg tgccgagcga ggaggagacc gcggcggttt cggacgcgga ttcggaggag 3000gtcgcggcga cagaggaggc cggggaggtc cgagaggccg aggcggcaga cgcggaggac 3060gtccgacaga ggaggagaag tggaccccag tgaccaagct cggccgtctc gtgcaagccg 3120gaaagatcca gaagctcgag cagatctacc tccactccct ccccgtcaag gagtaccaga 3180tcatcgacct cctcgtcggc cccaccttga aagacgaggt gatgaagatc atgccggttc 3240agaaacagac cagggccggt cagaggacga ggttcaaggc ctttgtcgtc gtcggagaca 3300cgaacgggca cgttggtttg ggtgtgaagt gctccaagga ggtggcgacg gcgatcagag 3360gtgggattat attggcgaag ctgtcggtta ttcccgtgag gagagggtac tgggggaaca 3420agattgggaa gccgcatacg gttccttgta aggttacggg gaagtgtggg tctgttacgg 3480tgaggatggt gcctgctccg aggggtgcgg ggattgtggc ggctagggtt cctaagaagg 3540tgcttcagtt cgctgggatt gatgatgtgt ttacttcgtc gagggggtct actaagactc 3600ttggaaactt cgtaaaggtt tgaactttct tcatttattt agagtttgga gttgtgtatg 3660gattgaaagt tgattccttt ttgatggttg cgtgtggagt tgttttgggt aatttagctt 3720gttgtagttg atgtaaagtg agaattgcta agcttgatta gtttgaataa gctgtctgtg 3780ttaaatgatg atttgtttgt gttgtattga gctgtctgaa tttcattatt tgtttgtgtt 3840attgctaaat tacaagctta aatttgataa cttttgattc tttcgtgaga gttttttttg 3900ggtaatatag cttgatgtag ttgatggaag taaattgtta acctttagag ctgtctgtta 3960atggtgattt gtttgtgttg tattgagctg tctgaattat aatttgtttg tgtttattgc 4020taaatcacaa agctttgaag tcgtttgtac atattcatca tttttgtgtt tattgacttg 4080tttgtgttta ttgctaaatc acaagtttct atgattaatc ttcatgattt ggttgtgttt 4140attactaaat cacaagtttc tatgattaat cttaatgatt tggttgtgtt tattgctaaa 4200tttgagatta cattataatc ttagtttata catgtataac atcatttcac ttgaattttc 4260acagacgagc tggtgatatg tttttttagt attttccatg tattgctttg taagaacttt 4320taaatgtttg ttttcttcct ttgaagaaca attaaatgtt tgttcagtat ttttgaatca 4380ttcattggtt aatcatgtgt aattttattt ttgtaggcta cattcgactg tctccagaag 4440acttatggat tcctgacacc agagttctgg aaagagacga gctttaaaaa gtcaccgtac 4500caggagtaca ctgatctgct ggccgagaag ggaattgcaa ccaaagccat caccgaggtt 4560gaagaccaag ccgcctccta aataaacaaa caaacaatgt cccccttgtt ttgtttttgg 4620atgatccttt cttgagtttt attttgttcg aacctttagt atatgttgtt tgctggattg 4680ctgctttctt atgttttcat catctccccc aaacaaagtt atttttgctc tagttttttt 4740ttataatcga tggttatgcc ctctagtatt ttttttactt gaatttttca ctaattttct 4800atcaacttcc ctatgttaca ctttctaagg ttgattacat aggtggattg ttgtgaaaca 4860aatactaata ataaactaat acttttagaa taatataatt agtgggatta aattataaat 4920tataaattaa aaagacaagt ctgaaatgga ttgtttaact gtaaaaggta aagagacttt 4980gaatagaaga gaataaatca aaagttgacg tatcttctcc gaaatttcag ctatttctaa 5040atacgcggat tctgattact ctgttctctc tcgcctgata aaccttagaa ttgagttttt 5100ttttttttta tctttttggt tcttttttag ttcttgtcgg aatcgtgaga agctctgtct 5160ctctgtcgga accaatgggg actccggagt ttccagatct ggggaaacac tgctccgtcg 5220atatctgcaa gcaaatcgat ttcttgcctt tcacctgcga ccgttgtctc caggttcctc 5280tctctctatc tctgttttga atcacatgca aaaaagaatc gtctttgttg cgaatagttg 5340tgtcgtatga ctgtctgaat cgcctgtctt cgatcctggg aactactata tcatcatcct 5400tgttcttttg tcgttcgtgt tctgggagaa ccaagtgaaa agtctccgtc tttctcttaa 5460ttaccgcgag gaaaattcat cttttctaca tgcgaccagt ttgatttgat ttgttccgac 5520attaagttta tcacttaagt tcttgtaatt acgaaccaac gtgttgagcc atctgtcatt 5580tcgagcttaa tcatcgacat ttcgcaattc tccgcactta aattcaccag agttgttcaa 5640aattcactta attcatcatg attgccatta aagattcgat ctttttgttg gtttacatta 5700acatatttga gtttatg 5717644463DNABrassica napus 64catatatgga tggacatagt tggacatatt taggtcctta tccaaatgct ggtgttaaga 60attgatttat ggatgtacat agttggatac attttatgtt taaaatgtga tttttatatt 120attattgctg tggtgtgaaa ttttgtgtta ttcacttaaa ttataatatc tatgtatacc 180aaagagaaga agataacata tattatagta tttggtacat actatactat aacatactta 240gtaaatttca caccacaaca aaaataatat aaaaggtcac attttaatat acaacgtgcc 300aaacttcgtc catttgtaat cttatacatt ttcgtcttcg catatgatag atcataagat 360ttggagaaac atataaaatt aacctatgaa attaaggggg tctatatctt atagtatgta 420tccaaagaac atatatagga aatatttgaa tacatcataa gatttgggga atataaatta 480atataaaata aaatcttcca attttaaacg taaacgtctc ctctgtattt atataaactt 540ttcaaaatca cggtgtcgct cgtgttaaga tttggatttc taatccaact ttcagaaagt 600atatgtgaaa gataaaactc aaacacaata gttcgtttag aacgctctta agattaatgt 660cacacacaca aaattgatgt cactagattt atttgttttg tcgtttaagg ggatccatca 720actacacatg gaagaataga aaacactggt cacacacaaa aaataatata agcatgttga 780attctattca tcaaagaatt ttcttcaaag gatgaacaat aacctataat taggaaacat 840tataacaacg agcaggaagt tttcccagaa gtcatgcctt cgtccatacc ttcgggtctt 900tcaagcaagc ttcacacatg ccttatcata tgtttgtata cgttttcatt ttgtgtacgt 960ctattaacta gctagtatat gccaagatta tatattttat atagtcgaca attacctatc 1020ctaaaagttc attttaattt ttattcagaa agatatgcac gtagggccac tagtgtttcc 1080cgttgttatt ggaaattcat ttccatgtgt tgaattgttt gtttaagttt ttgaacccgt 1140aagtcaaatt tattttggtt taacagggtt tcattactgt tataagcttg tcaagaaaga 1200atttgttaca aatttcgagg ctcatgcatg catgattctt aactttctag aaacatgaaa 1260tgtggtttta ccagctcaag cttgtgttaa aacacatttt taatttgacc acgtgtgata 1320ttcaaatatc aacaattctg ttcaacggga gattcataca tatgttcaga tgttctttaa 1380tcttaagcac ctaactttgc ttcccatacg caaatgtgtg tgccgcgaac tatggtgaaa 1440tgttttcttt ttggagcatt tttaaaagaa aatataattt acttatttcc gttttgattg 1500atatacatgc attaataaac cgacttacgt gggtgatggg atgagcaaaa ttaaaatatg 1560cattttagta accaattacg gtctatcttg acaaactaac aaacaaaaag tctattatga 1620cgttgatttg tgacctggcc atataaatgc caaaatttca ttctttcaat aagcaacgag 1680atcaataaga tgtggcagtc acgtgaacgt tagttatcgc catttcaact gaaccttcac 1740gaacatataa agttcatttg ttctttgcta aaattacgca aatctctgtt gtgcacgaaa 1800ttacgactat tttttaaata caactcctgt tcttgtatcg tgtatctctc gcacgtaaat 1860ttgaattatg ggggcattat cgataaaata tgtaaagaat atattttcat catacaccac 1920aaacaaatgt tctggaaatg tcacctttta tcacatatac aaagctgacc ctgaaatttt 1980ggaaactaca agccaaaaaa aatggtcaaa agaaatatta tgcacaaagg ttggactcgt

2040cctaactctt tttacaatga taattaacct ttgaacaact aattaaagat acatacaata 2100tgtttttttt ctaaaaattg gtcgattaaa tattcatata taatccttat catcatttat 2160tagccttttg tagcttactt aaaaaacccc ttcccaccaa attgattagt gtagattgat 2220tatttttcac ttcatacgtt gatcaatttt aaaatatata tatatatata tatatatata 2280tatatgcacg tccggcgtcc catgacttca gacgcttttc tgtttccttg attttatagg 2340ttaaaatttt ccaaaatgct catatttttc actcatatgt ataatgtatt attatacgca 2400caaaatagta aagtcataaa catttatgat ggctctttta tatgtataga gtagtgaaat 2460ttatatatat cggagttccg cttaaaataa attaatattg ttcttttatc gcccattttt 2520tagttactaa acgtatataa ttaaaaactg aaatgcaata tgtagaaatc cggcttatga 2580tgaaccatac taatactcat tgttacaaac tcacagcaac atcataattc catcactcgc 2640cacaataatt ttctttagtg tatgttctat ttcacatttt gacccttcac tttcttgaat 2700gaaaattata caagaaaatg atttgagtgt gttaaaaaaa aaatgttttg agtcattctg 2760accaattatc tggcagagac tataataaaa tacttccaat taccttaaat cattaggcgt 2820tgtttcttgc cgaatatact agggttttcc ctagctcacg ctaacacaca aagccgcagc 2880catttccatc tctctcgcaa ctcaacttcg aacaatggct gaacgaggcg gagaacgagg 2940cgccgagcga ggaggagacc gcggcggttt cggacgggga ttcggaggag gtcgcggcga 3000ccgaggaggc cgcggaggtc cgagaggccg aggcggcaga cgcggaggac gtcccacaga 3060ggaggagaag tggaccccag tgaccaagct cggccgtctc gtgcaagccg gaaagatcca 3120gaagctcgag cagatctacc tccactcgct ccccgtcaag gagtaccaga tcatcgacct 3180cctcgtcggt ccaactttaa aagacgaggt gatgaagatc atgccggttc agaaacagac 3240cagggccggt cagaggacga ggttcaaggc ttttgtcgtt gtcggagaca cgaatggtca 3300tgttggtttg ggtgtgaagt gctccaagga ggtggcgacg gcgatcagag gtgggattat 3360attggcgaag ctgtcggtga ttcccgtgag gagagggtac tgggggaaca agattgggaa 3420gccgcatacg gttccttgta aggttacggg gaagtgtggg tctgttacgg tgaggatggt 3480gcctgctccg aggggggcgg ggattgtggc ggctagggtt cctaagaagg tgcttcagtt 3540cgctgggatt gatgatgtgt ttacttcttc caggggatct actaagactc ttggaaactt 3600cgtaaaggtt tgaactttat tcatttactt ggtgtttgga gctgtgtata gattgaaagt 3660tgattccttt ttgatcgttg cgtgtggagg tgttttgggt aatattgcta aatgtaaaaa 3720ttgctaagct tgattagttt gaataagctg tctgtgttaa atgatgattt gtttgtgttg 3780taatgagctg tctgaatttc attatttgtt tgtgttattg ctaatttaca agcttaaatt 3840tgattccttt tgatcgtttc gtgtgagttg ttttgtgtaa tttagcttga tgtagttgat 3900ggaagtaaga attgttaagc tttagagctg tctgttaatg ttgaattgtt tgtgttgtat 3960tgagctgtct gaattattat ttgtttgcga tttatttcta aatcacaaag ctctaaagtc 4020gtttgaacat atgcatcatt tggttagtgt ttattgctga attttcatga tttggttgtg 4080tttattgcta aatttgagat tacattataa tcttaatttg tacatatatt catcattttt 4140gtgtttattg atttgtttgt gtttattgat gaatcacaag tttctatgat taatcttcat 4200gatttggttg tgattattgc taaatttgag attacattat aagcttagtt tgtacatgta 4260tacatcattt cacttgaatt ttcacagacg agctggtgat atattttttt ttttagtatt 4320ttccatgtat tgctttgtaa gaacgtttaa atgttagttt tcttgaatgt ttttttcttt 4380gtaagaacaa ttaaatgttt gttcagtatt tttgaatcat tcaatggtta atcatgttca 4440acatgtattt tttgtaggct aca 4463654789DNABrassica napus 65tgccttatga agatctacgt ctaactgcat catctgagcc atcctatgaa aatttatttt 60tgaccagatt tacttgcacc atgttgaaac ttctttataa acctttcttt tctagtttgc 120attaataaat cgctcttttc tctttctctg atacatatta ttcatgttgg ttatacctgt 180acacgcacgg acaatatttt ccaactttaa aacgaatcaa atcagttaat ttaataactt 240tggactagta actgtatttg aatttaaatt tctcttttca gaatattcta caaaacagtt 300ttaattccgt ttgtcagtag aatcacatat atggatggac atagttggac atatttaggt 360ccttatccaa atgctggtgt taagaattga tttatggatg tacatagttg gatacatttt 420atgtttaaaa tgtgattttt atattattat tgctgtggtg tgaaattttg tgttattcac 480ttaaattata atatctatgt ataccaaaga gaagaagata acatatatta tagtatttgg 540tacatactat actataacat acttagtaaa tttcacacca caacaaaaat aatataaaag 600gtcacatttt aatatacaac gtgccaaact tcgtccattt gtaatcttat acattttcgt 660cttcgcatat gatagatcat aagatttgga gaaacatata aaattaacct atgaaattaa 720gggggtctat atcttatagt atgtatccaa agaacatata taggaaatat ttgaatacat 780cataagattt ggggaatata aattaatata aaataaaatc ttccaatttt aaacgtaaac 840gtctcctctg tatttatata aacttttcaa aatcacggtg tcgctcgtgt taagatttgg 900atttctaatc caactttcag aaagtatatg tgaaagataa aactcaaaca caatagttcg 960tttagaacgc tcttaagatt aatgtcacac acacaaaatt gatgtcacta gatttatttg 1020ttttgtcgtt taaggggatc catcaactac acatggaaga atagaaaaca ctggtcacac 1080acaaaaaata atataagcat gttgaattct attcatcaaa gaattttctt caaaggatga 1140acaataacct ataattagga aacattataa caacgagcag gaagttttcc cagaagtcat 1200gccttcgtcc ataccttcgg gtctttcaag caagcttcac acatgcctta tcatatgttt 1260gtatacgttt tcattttgtg tacgtctatt aactagctag tatatgccaa gattatatat 1320tttatatagt cgacaattac ctatcctaaa agttcatttt aatttttatt cagaaagata 1380tgcacgtagg gccactagtg tttcccgttg ttattggaaa ttcatttcca tgtgttgaat 1440tgtttgttta agtttttgaa cccgtaagtc aaatttattt tggtttaaca gggtttcatt 1500actgttataa gcttgtcaag aaagaatttg ttacaaattt cgaggctcat gcatgcatga 1560ttcttaactt tctagaaaca tgaaatgtgg ttttaccagc tcaagcttgt gttaaaacac 1620atttttaatt tgaccacgtg tgatattcaa atatcaacaa ttctgttcaa cgggagattc 1680atacatatgt tcagatgttc tttaatctta agcacctaac tttgcttccc atacgcaaat 1740gtgtgtgccg cgaactatgg tgaaatgttt tctttttgga gcatttttaa aagaaaatat 1800aatttactta tttccgtttt gattgatata catgcattaa taaaccgact tacgtgggtg 1860atgggatgag caaaattaaa atatgcattt tagtaaccaa ttacggtcta tcttgacaaa 1920ctaacaaaca aaaagtctat tatgacgttg atttgtgacc tggccatata aatgccaaaa 1980tttcattctt tcaataagca acgagatcaa taagatgtgg cagtcacgtg aacgttagtt 2040atcgccattt caactgaacc ttcacgaaca tataaagttc atttgttctt tgctaaaatt 2100acgcaaatct ctgttgtgca cgaaattacg actatttttt aaatacaact cctgttcttg 2160tatcgtgtat ctctcgcacg taaatttgaa ttatgggggc attatcgata aaatatgtaa 2220agaatatatt ttcatcatac accacaaaca aatgttctgg aaatgtcacc ttttatcaca 2280tatacaaagc tgaccctgaa attttggaaa ctacaagcca aaaaaaatgg tcaaaagaaa 2340tattatgcac aaaggttgga ctcgtcctaa ctctttttac aatgataatt aacctttgaa 2400caactaatta aagatacata caatatgttt tttttctaaa aattggtcga ttaaatattc 2460atatataatc cttatcatca tttattagcc ttttgtagct tacttaaaaa accccttccc 2520accaaattga ttagtgtaga ttgattattt ttcacttcat acgttgatca attttaaaat 2580atatatatat atatatatat atatatatat gcacgtccgg cgtcccatga cttcagacgc 2640ttttctgttt ccttgatttt ataggttaaa attttccaaa atgctcatat ttttcactca 2700tatgtataat gtattattat acgcacaaaa tagtaaagtc ataaacattt atgatggctc 2760ttttatatgt atagagtagt gaaatttata tatatcggag ttccgcttaa aataaattaa 2820tattgttctt ttatcgccca ttttttagtt actaaacgta tataattaaa aactgaaatg 2880caatatgtag aaatccggct tatgatgaac catactaata ctcattgtta caaactcaca 2940gcaacatcat aattccatca ctcgccacaa taattttctt tagtgtatgt tctatttcac 3000attttgaccc ttcactttct tgaatgaaaa ttatacaaga aaatgatttg agtgtgttaa 3060aaaaaaaatg ttttgagtca ttctgaccaa ttatctggca gagactataa taaaatactt 3120ccaattacct taaatcatta ggcgttgttt cttgccgaat atagctaggg ttttccctag 3180ctcacgctaa cacacaaagc cgcagccatt tccatctctc tcgcaactca acttcgaaca 3240atggctgaac gaggcggaga acgaggcgcc gagcgaggag gagaccgcgg cggtttcgga 3300cggggattcg gaggaggtcg cggcgaccga ggaggccgcg gaggtccgag aggccgaggc 3360ggcagacgcg gaggacgtcc cacagaggag gagaagtgga ccccagtgac caagctcggc 3420cgtctcgtgc aagccggaaa gatccagaag ctcgagcaga tctacctcca ctcgctcccc 3480gtcaaggagt accagatcat cgacctcctc gtcggtccaa ctttaaaaga cgaggtgatg 3540aagatcatgc cggttcagaa acagaccagg gccggtcaga ggacgaggtt caaggctttt 3600gtcgttgtcg gagacacgaa tggtcatgtt ggtttgggtg tgaagtgctc caaggaggtg 3660gcgacggcga tcagaggtgg gattatattg gcgaagctgt cggtgattcc cgtgaggaga 3720gggtactggg ggaacaagat tgggaagccg catacggttc cttgtaaggt tacggggaag 3780tgtgggtctg ttacggtgag gatggtgcct gctccgaggg gggcggggat tgtggcggct 3840agggttccta agaaggtgct tcagttcgct gggattgatg atgtgtttac ttcttccagg 3900ggatctacta agactcttgg aaacttcgta aaggtttgaa ctttattcat ttacttggtg 3960tttggagctg tgtatagatt gaaagttgat tcctttttga tcgttgcgtg tggaggtgtt 4020ttgggtaata ttgctaaatg taaaaattgc taagcttgat tagtttgaat aagctgtctg 4080tgttaaatga tgatttgttt gtgttgtaat gagctgtctg aatttcatta tttgtttgtg 4140ttattgctaa tttacaagct taaatttgat tccttttgat cgtttcgtgt gagttgtttt 4200gtgtaattta gcttgatgta gttgatggaa gtaagaattg ttaagcttta gagctgtctg 4260ttaatgttga attgtttgtg ttgtattgag ctgtctgaat tattatttgt ttgcgattta 4320tttctaaatc acaaagctct aaagtcgttt gaacatatgc atcatttggt tagtgtttat 4380tgctgaattt tcatgatttg gttgtgttta ttgctaaatt tgagattaca ttataatctt 4440aatttgtaca tatattcatc atttttgtgt ttattgattt gtttgtgttt attgatgaat 4500cacaagtttc tatgattaat cttcatgatt tggttgtgat tattgctaaa tttgagatta 4560cattataagc ttagtttgta catgtataca tcatttcact tgaattttca cagacgagct 4620ggtgatatat tttttttttt agtattttcc atgtattgct ttgtaagaac gtttaaatgt 4680tagttttctt gaatgttttt ttctttgtaa gaacaattaa atgtttgttc agtatttttg 4740aatcattcaa tggttaatca tgttcaacat gtattttttg taggctaca 4789664465DNABrassica napus 66catatatgga tggacatagt tggacatatt taggtcctta tccaaatgct ggtgttaaga 60attgatttat ggatgtacat agttggatac attttatgtt taaaatgtga tttttatatt 120attattgctg tggtgtgaaa ttttgtgtta ttcacttaaa ttataatatc tatgtatacc 180aaagagaaga agataacata tattatagta tttggtacat actatactat aacatactta 240gtaaatttca caccacaaca aaaataatat aaaaggtcac attttaatat acaacgtgcc 300aaacttcgtc catttgtaat cttatacatt ttcgtcttcg catatgatag atcataagat 360ttggagaaac atataaaatt aacctatgaa attaaggggg tctatatctt atagtatgta 420tccaaagaac atatatagga aatatttgaa tacatcataa gatttgggga atataaatta 480atataaaata aaatcttcca attttaaacg taaacgtctc ctctgtattt atataaactt 540ttcaaaatca cggtgtcgct cgtgttaaga tttggatttc taatccaact ttcagaaagt 600atatgtgaaa gataaaactc aaacacaata gttcgtttag aacgctctta agattaatgt 660cacacacaca aaattgatgt cactagattt atttgttttg tcgtttaagg ggatccatca 720actacacatg gaagaataga aaacactggt cacacacaaa aaataatata agcatgttga 780attctattca tcaaagaatt ttcttcaaag gatgaacaat aacctataat taggaaacat 840tataacaacg agcaggaagt tttcccagaa gtcatgcctt cgtccatacc ttcgggtctt 900tcaagcaagc ttcacacatg ccttatcata tgtttgtata cgttttcatt ttgtgtacgt 960ctattaacta gctagtatat gccaagatta tatattttat atagtcgaca attacctatc 1020ctaaaagttc attttaattt ttattcagaa agatatgcac gtagggccac tagtgtttcc 1080cgttgttatt ggaaattcat ttccatgtgt tgaattgttt gtttaagttt ttgaacccgt 1140aagtcaaatt tattttggtt taacagggtt tcattactgt tataagcttg tcaagaaaga 1200atttgttaca aatttcgagg ctcatgcatg catgattctt aactttctag aaacatgaaa 1260tgtggtttta ccagctcaag cttgtgttaa aacacatttt taatttgacc acgtgtgata 1320ttcaaatatc aacaattctg ttcaacggga gattcataca tatgttcaga tgttctttaa 1380tcttaagcac ctaactttgc ttcccatacg caaatgtgtg tgccgcgaac tatggtgaaa 1440tgttttcttt ttggagcatt tttaaaagaa aatataattt acttatttcc gttttgattg 1500atatacatgc attaataaac cgacttacgt gggtgatggg atgagcaaaa ttaaaatatg 1560cattttagta accaattacg gtctatcttg acaaactaac aaacaaaaag tctattatga 1620cgttgatttg tgacctggcc atataaatgc caaaatttca ttctttcaat aagcaacgag 1680atcaataaga tgtggcagtc acgtgaacgt tagttatcgc catttcaact gaaccttcac 1740gaacatataa agttcatttg ttctttgcta aaattacgca aatctctgtt gtgcacgaaa 1800ttacgactat tttttaaata caactcctgt tcttgtatcg tgtatctctc gcacgtaaat 1860ttgaattatg ggggcattat cgataaaata tgtaaagaat atattttcat catacaccac 1920aaacaaatgt tctggaaatg tcacctttta tcacatatac aaagctgacc ctgaaatttt 1980ggaaactaca agccaaaaaa aatggtcaaa agaaatatta tgcacaaagg ttggactcgt 2040cctaactctt tttacaatga taattaacct ttgaacaact aattaaagat acatacaata 2100tgtttttttt ctaaaaattg gtcgattaaa tattcatata taatccttat catcatttat 2160tagccttttg tagcttactt aaaaaacccc ttcccaccaa attgattagt gtagattgat 2220tatttttcac ttcatacgtt gatcaatttt aaaatatata tatatatata tatatatata 2280tatatgcacg tccggcgtcc catgacttca gacgcttttc tgtttccttg attttatagg 2340ttaaaatttt ccaaaatgct catatttttc actcatatgt ataatgtatt attatacgca 2400caaaatagta aagtcataaa catttatgat ggctctttta tatgtataga gtagtgaaat 2460ttatatatat cggagttccg cttaaaataa attaatattg ttcttttatc gcccattttt 2520tagttactaa acgtatataa ttaaaaactg aaatgcaata tgtagaaatc cggcttatga 2580tgaaccatac taatactcat tgttacaaac tcacagcaac atcataattc catcactcgc 2640cacaataatt ttctttagtg tatgttctat ttcacatttt gacccttcac tttcttgaat 2700gaaaattata caagaaaatg atttgagtgt gttaaaaaaa aaatgttttg agtcattctg 2760accaattatc tggcagagac tataataaaa tacttccaat taccttaaat cattaggcgt 2820tgtttcttgc cgaatataag ctagggtttt ccctagctca cgctaacaca caaagccgca 2880gccatttcca tctctctcgc aactcaactt cgaacaatgg ctgaacgagg cggagaacga 2940ggcgccgagc gaggaggaga ccgcggcggt ttcggacggg gattcggagg aggtcgcggc 3000gaccgaggag gccgcggagg tccgagaggc cgaggcggca gacgcggagg acgtcccaca 3060gaggaggaga agtggacccc agtgaccaag ctcggccgtc tcgtgcaagc cggaaagatc 3120cagaagctcg agcagatcta cctccactcg ctccccgtca aggagtacca gatcatcgac 3180ctcctcgtcg gtccaacttt aaaagacgag gtgatgaaga tcatgccggt tcagaaacag 3240accagggccg gtcagaggac gaggttcaag gcttttgtcg ttgtcggaga cacgaatggt 3300catgttggtt tgggtgtgaa gtgctccaag gaggtggcga cggcgatcag aggtgggatt 3360atattggcga agctgtcggt gattcccgtg aggagagggt actgggggaa caagattggg 3420aagccgcata cggttccttg taaggttacg gggaagtgtg ggtctgttac ggtgaggatg 3480gtgcctgctc cgaggggggc ggggattgtg gcggctaggg ttcctaagaa ggtgcttcag 3540ttcgctggga ttgatgatgt gtttacttct tccaggggat ctactaagac tcttggaaac 3600ttcgtaaagg tttgaacttt attcatttac ttggtgtttg gagctgtgta tagattgaaa 3660gttgattcct ttttgatcgt tgcgtgtgga ggtgttttgg gtaatattgc taaatgtaaa 3720aattgctaag cttgattagt ttgaataagc tgtctgtgtt aaatgatgat ttgtttgtgt 3780tgtaatgagc tgtctgaatt tcattatttg tttgtgttat tgctaattta caagcttaaa 3840tttgattcct tttgatcgtt tcgtgtgagt tgttttgtgt aatttagctt gatgtagttg 3900atggaagtaa gaattgttaa gctttagagc tgtctgttaa tgttgaattg tttgtgttgt 3960attgagctgt ctgaattatt atttgtttgc gatttatttc taaatcacaa agctctaaag 4020tcgtttgaac atatgcatca tttggttagt gtttattgct gaattttcat gatttggttg 4080tgtttattgc taaatttgag attacattat aatcttaatt tgtacatata ttcatcattt 4140ttgtgtttat tgatttgttt gtgtttattg atgaatcaca agtttctatg attaatcttc 4200atgatttggt tgtgattatt gctaaatttg agattacatt ataagcttag tttgtacatg 4260tatacatcat ttcacttgaa ttttcacaga cgagctggtg atatattttt ttttttagta 4320ttttccatgt attgctttgt aagaacgttt aaatgttagt tttcttgaat gtttttttct 4380ttgtaagaac aattaaatgt ttgttcagta tttttgaatc attcaatggt taatcatgtt 4440caacatgtat tttttgtagg ctaca 4465676454DNABrassica napus 67aagccagaaa aaaatgtgga agtgtaataa aacaattttc tccaggaaga agatatttca 60gccattcgcc tatttatgtg tatgagatcc cattcaagga catactgttt tccgtgagct 120actgaatgaa tacgatgctt ttgttgtaaa cgcaacctat tggttttttt tttccgccaa 180gttttgtttt tattaatcaa acgggccaag cccaaacggt cgaagcccaa atacactaag 240taccaaacaa aaggcccaca gacacaacgg gctacaacat taatggctga agcccaagta 300aactaaacgg cccaaaggcc caaacattga aaccggacag cttgacctag gcgcgcgata 360aggagactgg ggtttgaaca cgtgtaagaa tctgggccat tagtatgaca cgcgtcgccc 420gcctcgactt gatcgaaacc tcttcgcaca gccgcatgaa cttgccaccg acgacctcgt 480ttcgccggag ctcagagact gaagagcctc caacgaaacc atagctattc tacgccgctc 540tgtcttcacg ccggagagtt ccatcgatct aacgagatct cctccgacta tgtatcacca 600caaaccctag acaggcgagc cgagaaccac tgatatcctc ctcttgtacg tcctcgccgt 660tgtcggagag tttcatcatc atccgagatc tcatccgcga tggcccacga ccccatacct 720gaccacaaga accacaccgc acttatctca cctcgatcta atcaccacaa cccaaaggac 780atcgggttcc aagacggcag cgcggagctt tgcaagcctc cactctccgt gaccaaaagc 840cggcgaagac ggagctgaat gagcctcccc ttcccggaag ctaaagcggc gacggtggat 900ctgagaaagc ctccacctcc cgcataaaga ccggcgacga tggatctagg gtagcctcca 960tctcccggaa aaaacaccac ctgaacatgc aagccgctcc atctccaccg ggagcctcca 1020attgatcgcg tcgttactcc aaaggccgag ccaccgttga tggatggttg cggtcccaga 1080gctccgacaa ggcgatcgat gacgggactg cgaagaagga gcggagagtg acaactacaa 1140aggaagggaa agagctctgg cgacagcacg gacgctcacg cgccagccgt acgccgaacc 1200caaaagtaga tctggtttct ctcttttctc tctctcgcaa aagaaaacac gcaacctatt 1260gttctccatt ttgaatttga agtgttacat tattaataaa aaggaagaaa gtacaaagct 1320tttcatttag tggttgttgc atatatagta tacttatttg atgggcttat gataagtatg 1380aacatttcac gtaacgggtt tattttgtcg tcaacacatt accggcaatg tgaaaagaag 1440gggcgaggta tctccatttc aattcttctc tttatagatt aatcgaatta tttacatatg 1500aaatgagcgt ttatatagaa ttttcgtgtg gaaaacgaca tgtacacggc atcgaaagac 1560caattagtaa tatactttag tggtgattac atgtttactt atccaattga gaatttaaag 1620catcgacaat accttaatgt cgattaagcc gtccccactt catgtaatga gttatggggg 1680gagagagagg tcccgaaatt cgtcaaataa aacaacttag aactaaaaac cgacaccaag 1740tatcataaag gaaacgttga agaagtcatt tatcgtatcc agctcacaat tcctaagatt 1800aaatcatgac cgttggaaga gcttataaga ttaaattgaa gaaattgtgg gttttagaag 1860aaagacaaga aagagaagaa catgatctta cattgcctat tttggtgtat aggagttgtc 1920aaaaagagga gagagaggag acaattaggt caaataaatg agcactaaaa atggagacat 1980gtgttgagta actattacaa gagcgactta tgcttctata tggcaatgat atcatcacca 2040aagtgcaatg cccctttttg ccctagtttc gtaaagtctc tttccttctt catccttagg 2100aaaaacccta aattaaatcc tgtgttcttg atcttgcttt ttgagtaacc atgattttga 2160ccacacacta gttcttctat attttgtggt ctataggatt ttgctttata tgtgtttctt 2220gtattgcacc gtacgtgcgt atataaattt aaatggttac aacaaggttt attataaaca 2280ggcacaaatt agtccatgag gttatttagc ttgcacaaga ggtatataaa tgtgtgtgtg 2340tgtgttaagt atttaaatat ataaatttgc tacaaaactt aattaacttt atctgattat 2400attttcttta gtgttcttca tttgccaacg ttgaggtagc tattattatt attattatta 2460ttattattat tattattatt attattatta ttattattat tattattatt attattattt 2520tgaacattat gtactatgta cgtagttatt ttggctagtt atgattcaaa ttcttaattt 2580ggataacact taacagtatt taaaaaaaaa atactattta aaatattctt agtactaaaa 2640taattaagat ttaacttgaa attgaagtat tcgtgttaaa cagaaactac aataaacaaa 2700tgattgcatg ttaatttttt tttcgattat cctatatatc agaataaaca catgattgca 2760tgcaaatttt gtttttgatt atgtcatctt ttgtttattt tagttttgat gctaattaat 2820atttttttta ttaacaactt ccatacattc tacctgattc taggtcagat aatgacacag 2880cgcaacaaaa ttatacaaaa ctttcggaaa gtagattacc gcggaagtaa catttttggg 2940tagacatacg aagtagtttg aatcttcaaa aatcatgcca tacataacca cggatcatag 3000tcgacacctc aacgtgaagc aaatttgaca atctacatac ataaccaaca aaaagtagaa 3060taccgtgaaa acctaaaccc aaaatatgat gtaaaactca agcttggtcc agagcataaa 3120aaaattaaag ccatcgcttt ggtatcacat atttaaacgt cagttttttt ttggggaagt 3180aatataaaaa tataattaac aagaaaattt atgaaataat

tagcatgtaa aacactagtc 3240ttttggttgt gacaaaacgt tttcacaaat gttctataaa taaattcaag cacattttat 3300ctgcaaaata tatactttca ctcataaaat aagagcgttt aaaacattca tatacgcact 3360acattgacat gacaaaagaa atccgcaaat acaaacatat ttagttcgga tatatctagg 3420aaataagact atattatata tataaagaaa ttagaaaaaa agaaaattgg tatgtataaa 3480agaaaggtct atgcgtctct agtccaaaaa ctctatatgt ctggttcaaa agcagatgca 3540gcagccatag ccccaatagt catgatggag cctcatcatc tccttatgaa ctggaacaaa 3600cctattgatc tcattacaca agaaaactct tttaaccaca atcctcattt catggtagat 3660ccaccttccg aaaccctaag ccacttccag cccccgccga cagtcttctc cgatcccgga 3720ggaggagagg aagcagaaga cgaagaagga gaggaagaga tagatgagat gaaggagatg 3780caatacgcga ttgctgccat gcagcccgta gacatcgatc cagccaccgt tcctaagccg 3840aaccgccgta acgtaagggt aagcgaggac ccccagacgg tggtggctcg tcggcgtaga 3900gaaaggataa gcgagaagat ccggatattg aagaggatgg tgccaggcgg tgcaaagatg 3960gacactgcct ccatgcttga cgaagccatc cgctacacca agttcttgaa acggcaggtg 4020aggcttcttc agcctcacac tcagcttggg gctcctatgt ctgacccttc tcgcctttgt 4080tattaccaca actcggatac ctaattataa ttctatcacg cgtttcatgt tgatatatat 4140agataaatgg ttgaataagg atttcgatcg aagattgtat ggctattgat tacattatat 4200attgtacaat aaatgatgtg tgtatttcta ttaatgtata tatgatatat atctgtttgc 4260agtatgcatt tatattctat tctttatagg gaggcaacat gccggattag ggctttgatc 4320gtatgcaagt tttccgacca aaaatatgaa atacttgttt ggatataaca tatgaatcgg 4380ataagtgtta ctagttatat aactggaaaa aaattgtttg gtataagaat tcccgggaga 4440accaagcctt tctctaatcc ctaagatcat agctactgaa ataatgaaaa aaaacaaaaa 4500aaaaacaatg aagaatcagt tgggcattag tccaaaaaaa aaaaagaatc agttggattg 4560cttataaaat tttgttataa aatttatgtc gtatgtgtgt tagccgtagc tgtaaattta 4620tttcctttgt atgttaattg taaaatttaa tttattattc ccgatgtttt taatgatgta 4680agtttacatc aagaaaacat attaagttct gttaataaat gtattatttt tgtttactaa 4740tttccaataa ttttttcaag aagtacaatt ttttgtttga aatttctaat ttatcaacat 4800acctaaaaac cataaacatg tattttttga aacatttttt taatataaaa attatttttt 4860aggagcaggt gaagtatttc ttttaataaa attgttagtg tatttacgtt ggttatagtt 4920gtagatattt tgcataataa ttaataatat ataagatgtg taatgtatat atgtgagaga 4980aataccatat ttactaagag tttttcgcaa tattgactca aaattcaaag tcaatcacaa 5040aactaacata tgtttctttt tagacatttc ctttgcctta ttcactccac gggttcagat 5100tattcacgaa aatgtcatta aatttttttt cttttcgaaa atggcatttt tactctctca 5160atttcatcct cttcaagtat ttacaagatt tccattgcca tcaatacacc aaccacaatg 5220aacaaccaat ttgaagctct taatgcacct caaatcgatt tacacttctt ctttttcaat 5280tcttatgaac taaaaacaac atctctttca atttctcccc atattcatcc aaaaaaccca 5340agatttttat tctaaaattt taatggttca tagagccatt gaagcttacg attcttggtg 5400ggtcactttt gttcgagatt ttaggtactt ggagaagact tttgtatgct aaacaagtta 5460tctcattggc tgaaactatg aaatcagttt tttttccaga tctgttcgcc cggacgactt 5520ccggataagt cttctggctg tagacgactt acatcgaagt attctggtta atgcagatgt 5580tagttttgca attgactttt aagatgctta ctcgaagatt agtactcttc tgttcttgta 5640ccttatgagc attccaatag tattatattt ttcattctaa aatatatttc aatgatattt 5700tacttttcac tctataatag agtgaaaatg agtttacttt ataatcccct atatattatt 5760tgtgaaacac tacaacttct ttttgtagcc acatgtcatc actaggatga tttttagaat 5820tattagagaa ataggttggt ccatctaatt atataataag ttttttatta aattaaccat 5880aaattcatta ttaatgtcat ttattatttc cttaaataaa gattacggaa ttgcctaatg 5940tgggtaaagt atatatgaca attaatgatc ttgaataata aagatatgat aaaaaaaatg 6000tatcttctat caaatttgtt taatttaaaa ctattaaaat aatttttaaa aaaacacaat 6060aaccatatta taaaaattta gatttttctg tatattttta tattttgaac tttaaaaaat 6120gacgataaat tactaaaact gttaaaagtc tcacattcaa attttgctat ccatgtttta 6180aaatttttgt tatgacaaaa tacaaatgat tacaaaatca tataagtaaa agtctaattt 6240aattaatcat taagatttaa aatatatatg tatatatatc attctaaatt aaactataaa 6300ccatattgaa taaataaata ttttagtttc aaaattttac tttgaataat tttttttgat 6360aaaagttttg aactaacatt gataaatgtt tttaaactat caattaccaa aattattgat 6420cccacaatga aaattttgtt atcagtaatt taaa 6454684772DNABrassica napus 68gaattacgaa aacaaaattt gaaaaaaaaa taataaaaaa tttcaaaaaa aaattataaa 60aagttcgaat ttgaaaacat ataatctgaa actataaaaa ttaattatta ttattattat 120ttgtatttat atatctaggg tattaaaatc tttttactta ttttatgaaa cattttggtt 180atctttctcc ttacaatcta tttttgtgat aaaaacttga aaatagtcta cttaggagaa 240ttacccttta cattatctaa taaactgatc ttctagaaga gagaaacgtt aatggtggac 300gaagccccca cctcaattga caataaaaga aagtggaaaa gagagaagat agcagagtga 360aaggtccgaa gagaggagat catgaagaga agaagacaat gtaacctact tatttgcctt 420gatgaacaat tacgagaaga aataaacata aaatgagaag agagaggtgg gatatacaga 480agagagaaag atttggcagg aaacccgatt gaccatatat cagatctaga gtggattgga 540ttagataact cgagcttatt tggttaatct ttttagttta gggaaggtta tgaaaaatta 600accccgatct gatttttaaa ttttttatct tatattgtgc ccccggctaa caatatatct 660agatccgtcc ctgatcaggt ccatgagata tatcatattt ttatttttct tatttaatta 720attatatata taatatttga ggatatattt aaaattttac atatcgttga aagccagaaa 780aaaatgtgga agtgtaataa aacaattttc tccaggaaga agatatttca gccattcgcc 840tatttatgtg tatgagatcc cattcaagga catactgttt tccgtgagct actgaatgaa 900tacgatgctt ttgttgtaaa cgcaacctat tggttttttt tttccgccaa gttttgtttt 960tattaatcaa acgggccaag cccaaacggt cgaagcccaa atacactaag taccaaacaa 1020aaggcccaca gacacaacgg gctacaacat taatggctga agcccaagta aactaaacgg 1080cccaaaggcc caaacattga aaccggacag cttgacctag gcgcgcgata aggagactgg 1140ggtttgaaca cgtgtaagaa tctgggccat tagtatgaca cgcgtcgccc gcctcgactt 1200gatcgaaacc tcttcgcaca gccgcatgaa cttgccaccg acgacctcgt ttcgccggag 1260ctcagagact gaagagcctc caacgaaacc atagctattc tacgccgctc tgtcttcacg 1320ccggagagtt ccatcgatct aacgagatct cctccgacta tgtatcacca caaaccctag 1380acaggcgagc cgagaaccac tgatatcctc ctcttgtacg tcctcgccgt tgtcggagag 1440tttcatcatc atccgagatc tcatccgcga tggcccacga ccccatacct gaccacaaga 1500accacaccgc acttatctca cctcgatcta atcaccacaa cccaaaggac atcgggttcc 1560aagacggcag cgcggagctt tgcaagcctc cactctccgt gaccaaaagc cggcgaagac 1620ggagctgaat gagcctcccc ttcccggaag ctaaagcggc gacggtggat ctgagaaagc 1680ctccacctcc cgcataaaga ccggcgacga tggatctagg gtagcctcca tctcccggaa 1740aaaacaccac ctgaacatgc aagccgctcc atctccaccg ggagcctcca attgatcgcg 1800tcgttactcc aaaggccgag ccaccgttga tggatggttg cggtcccaga gctccgacaa 1860ggcgatcgat gacgggactg cgaagaagga gcggagagtg acaactacaa aggaagggaa 1920agagctctgg cgacagcacg gacgctcacg cgccagccgt acgccgaacc caaaagtaga 1980tctggtttct ctcttttctc tctctcgcaa aagaaaacac gcaacctatt gttctccatt 2040ttgaatttga agtgttacat tattaataaa aaggaagaaa gtacaaagct tttcatttag 2100tggttgttgc atatatagta tacttatttg atgggcttat gataagtatg aacatttcac 2160gtaacgggtt tattttgtcg tcaacacatt accggcaatg tgaaaagaag gggcgaggta 2220tctccatttc aattcttctc tttatagatt aatcgaatta tttacatatg aaatgagcgt 2280ttatatagaa ttttcgtgtg gaaaacgaca tgtacacggc atcgaaagac caattagtaa 2340tatactttag tggtgattac atgtttactt atccaattga gaatttaaag catcgacaat 2400accttaatgt cgattaagcc gtccccactt catgtaatga gttatggggg gagagagagg 2460tcccgaaatt cgtcaaataa aacaacttag aactaaaaac cgacaccaag tatcataaag 2520gaaacgttga agaagtcatt tatcgtatcc agctcacaat tcctaagatt aaatcatgac 2580cgttggaaga gcttataaga ttaaattgaa gaaattgtgg gttttagaag aaagacaaga 2640aagagaagaa catgatctta cattgcctat tttggtgtat aggagttgtc aaaaagagga 2700gagagaggag acaattaggt caaataaatg agcactaaaa atggagacat gtgttgagta 2760actattacaa gagcgactta tgcttctacc aagcctttct ctaatcccta agatcatagc 2820tactgaaata atgaaaaaaa acaaaaaaaa aacaatgaag aatcagttgg gcattagtcc 2880aaaaaaaaaa aagaatcagt tggattgctt ataaaatttt gttataaaat ttatgtcgta 2940tgtgtgttag ccgtagctgt aaatttattt cctttgtatg ttaattgtaa aatttaattt 3000attattcccg atgtttttaa tgatgtaagt ttacatcaag aaaacatatt aagttctgtt 3060aataaatgta ttatttttgt ttactaattt ccaataattt tttcaagaag tacaattttt 3120tgtttgaaat ttctaattta tcaacatacc taaaaaccat aaacatgtat tttttgaaac 3180atttttttaa tataaaaatt attttttagg agcaggtgaa gtatttcttt taataaaatt 3240gttagtgtat ttacgttggt tatagttgta gatattttgc ataataatta ataatatata 3300agatgtgtaa tgtatatatg tgagagaaat accatattta ctaagagttt ttcgcaatat 3360tgactcaaaa ttcaaagtca atcacaaaac taacatatgt ttctttttag acatttcctt 3420tgccttattc actccacggg ttcagattat tcacgaaaat gtcattaaat tttttttctt 3480ttcgaaaatg gcatttttac tctctcaatt tcatcctctt caagtattta caagatttcc 3540attgccatca atacaccaac cacaatgaac aaccaatttg aagctcttaa tgcacctcaa 3600atcgatttac acttcttctt tttcaattct tatgaactaa aaacaacatc tctttcaatt 3660tctccccata ttcatccaaa aaacccaaga tttttattct aaaattttaa tggttcatag 3720agccattgaa gcttacgatt cttggtgggt cacttttgtt cgagatttta ggtacttgga 3780gaagactttt gtatgctaaa caagttatct cattggctga aactatgaaa tcagtttttt 3840ttccagatct gttcgcccgg acgacttccg gataagtctt ctggctgtag acgacttaca 3900tcgaagtatt ctggttaatg cagatgttag ttttgcaatt gacttttaag atgcttactc 3960gaagattagt actcttctgt tcttgtacct tatgagcatt ccaatagtat tatatttttc 4020attctaaaat atatttcaat gatattttac ttttcactct ataatagagt gaaaatgagt 4080ttactttata atcccctata tattatttgt gaaacactac aacttctttt tgtagccaca 4140tgtcatcact aggatgattt ttagaattat tagagaaata ggttggtcca tctaattata 4200taataagttt tttattaaat taaccataaa ttcattatta atgtcattta ttatttcctt 4260aaataaagat tacggaattg cctaatgtgg gtaaagtata tatgacaatt aatgatcttg 4320aataataaag atatgataaa aaaaatgtat cttctatcaa atttgtttaa tttaaaacta 4380ttaaaataat ttttaaaaaa acacaataac catattataa aaatttagat ttttctgtat 4440atttttatat tttgaacttt aaaaaatgac gataaattac taaaactgtt aaaagtctca 4500cattcaaatt ttgctatcca tgttttaaaa tttttgttat gacaaaatac aaatgattac 4560aaaatcatat aagtaaaagt ctaatttaat taatcattaa gatttaaaat atatatgtat 4620atatatcatt ctaaattaaa ctataaacca tattgaataa ataaatattt tagtttcaaa 4680attttacttt gaataatttt ttttgataaa agttttgaac taacattgat aaatgttttt 4740aaactatcaa ttaccaaaat tattgatccc ac 4772696656DNABrassica napus 69aaaaatattt gatttatata ttcttattaa aaatattaat ttactgttta ttattttata 60taattttata aatcatttta tttagagttt aatttattta cgatttattg ttttaatttg 120ttaggcaaat tagtctaaca aattaaacaa aaaaaaatat aaataaatta aattttaaac 180aaaatgtttt ataaaaatat ttatatcctt ataaggatat aaactataat tttgtgttat 240ttttatattt aaactattat tatatttaat tatttttaaa taaatttatt tatatttttg 300tatattttta ttaaaactaa tattttattt aattatgaaa attgaaaacg tttttttggt 360tatttttgaa aattttgaca aggttgaaaa atcgctggaa atatttttga ggaattttag 420acaaaaaaat atcgatgata aacaattggt tggaatccgt caagaatttt tgacaaaata 480aactcgaaat ttgttttcac tgggaattag ttattttctt gtatttgcat tatctaataa 540acaatgcaac cataaacaac aaatgaaata aagttttaat tgacaaatct tttagaagag 600tgaaacgtta atggtggacg aagcccccac ctcaattgac aataaaaaga gtggaaaaga 660gggaagatgg cagagagaaa agtccaaaga gaggaaatca tgaagagaag aagacaatgt 720aacctactta tttgcctcga tgaagataga ggagaagata gaggtggaat atatatagaa 780gagagaaaga tttgggagga aacccgattg agcatatatc agatctagaa tggattggat 840tagataactc gaacttattt ggttaatctt tttagtttag ggaaggttat gaaaaattac 900ctctgatcta atttttaaat tttttatctt atatacattg tgccccccga ctaacaatat 960atctagatcc atccctgatt aggtccatga gatatatcat attttttatt tttcttattt 1020aattaattat ctataatatt tgttgatata tttaaaattt tacatatcgt tgaaagccag 1080aaaaatatgt ggaagtgtaa taaaacaatt ttctccagga agaagatatt tcagccattc 1140gcccatctat gtgtatgaga tcccattcaa ggccatattc taagattgac tttatctaat 1200ttactgtatt ttccgtgggc tactgaatga atacgatgct tttgttgtaa aacgcaacct 1260attgttctcc attttgaatt tgaagtgtta cattgttgat aaaaaggaag aaagtacaaa 1320gcttttcatt tagtggttgt tgcatatata tacttatttg atgggtttat gataagtatg 1380aacatttcac gtaacgggtt tattttgtcg tcaacacatt atcggcaatg tgaaaagaag 1440gggtgaggta tctccatttc aattcttctc tttatatatt aatcgaatta tttacgtatg 1500aaatgaacgt ttatatagaa atttcgtgtg gaaaacgaca tgtacacggc atctcaagac 1560caattagtaa tatactttag tggtgattac atgtttactt atccaattga gaatttaaag 1620catcgacaat accttaatgt cgattaagcc gtccccactt catgtaatga gttatggggg 1680gagagagaga tcccgaaatt cgtcaaataa aacaacttag aactaaaaac cgacaccaag 1740tatcataaag gaaatgttga agaagtcatt tatcgtatcc agctcacaat tcctaagatt 1800aaatcatgac cgttggaaga gcttataaga ttaaactgaa gaaattgtgg gttttagaag 1860aaagacaaga aagagaagaa catgatctta cattgcctat tttggtgtat aggagttgtc 1920aaaaagagga gagagaggag acaattaggt caaataaatg agcactaaaa atggagacat 1980gtgttgagta actattacaa gagcgactta tgcttctata tggcaatgat atcatcacca 2040aagtgcaatg cccctttttg ccctagtttc gtaaagtctc tctccttctt cgtccttagg 2100aaaaacccta aattaaatcc tgtgttcttg atctttcttt ttgagtaacc atgattttga 2160ccacacacta gttcttctat attttgtggt ctataggatt ttgctttata tgtgtttctt 2220gtattgctcc gtacgtgcgt atataaattt aaatggttac aacaaggttt attataaata 2280ggcacaaatt agtccatgaa gttatttagc ttgcacaagt ataatttgtt aagtatttaa 2340atatataaat ttgttacaaa acttaattaa atttatctga ttatattttc tttagtgttc 2400ttcctttgcc aacgttgagg tagctattat tattattatt ttgaacatta tgtacgtagt 2460tatcttggct agttatgatt cgaattctta atttggatca cacttaacag tatttaaaat 2520attcttagaa ctaaaataat taagagttac ctttaaattg aagtattcgt gctaaacaga 2580aactagaata aacaaatgat tgcatgttaa tttttttttt cgattttcct atcagaataa 2640acacatgatt gcatgcaaat attgtttttg attacgttat cttttgttta ttttagtttt 2700gatgctaatt aatatttttt attaacaact cacatacatt ctacctgatt ctaggtcaga 2760taatgacaca gcgcaacaaa attaatacaa aaccttcgga aagtagaata ccgcagaagt 2820aacttttttg ggtacatacg aaatacagtg aaatctctat aaattaataa tgttgagact 2880ataccaaaac tataaaattt ttattaattt atagagatta atttatcgat atactaattg 2940aatcaaaaac ttaatttgag attaaaaaat tatattattt tatagagatt tttagtgtat 3000attaatttat agaatattat tttataaaaa attttagtgt gtattaattt atagagtatt 3060aatttaaaga agttatactg taatgtgaat cttcgaaaaa catgccatac ataaccacgg 3120atcatagtcg acacctcaac gtgaagcaaa tttgacaatc tacatacata accaacaaaa 3180agtagaatac cttgaaaatc taaaacccaa aatatgatgt aaaactcaag cttggtccag 3240agcataaaaa aattaaagcc atcgctttgg tatcacatat ttaaacgtca gttttttttt 3300tttttttggg gggggggggg gggggtaata taaaaatata attaacaaaa acaatttatg 3360aaacaattag catgtaaaac actaatcttt tggttgtgac aaaacgtttt cacaaatgtt 3420ctataaataa attcaagtgc attttatctg caaaatatat actttcactc ataaaataag 3480agcgtttaaa acattcatac acgcactaca ttgacatgac aaaagaaatc cgcaaataca 3540catgatgtat gtcgaaaaaa acaaaaaaat acacatgatg tatatataga gaggatagta 3600tctaggaaat aagactatat tatatatata aagaaaatag agaaaagata aaaatataaa 3660ttggtatgta taaaataaag gtctatgcgt ctctagtcca aaaactctat atatgtctgg 3720ctcaaaagca gatgcagcca tagccccaat agtcatgatg gagcatcatc atctccttat 3780gaattggaac aaacctattg atctcattac agaagaaaac tcttttaacc acaatcctca 3840tttcatagta gatccacctt ccgaaaccct aagccacttc cagcccccgc cgacaatctt 3900ctccgatcac ggaggaggag aggaagcaga agaagaagaa ggagaggaag agatggatcc 3960gatgaagaag atgcaatacg cgattgctgc catgcagccc gtagacctcg atccagccac 4020cgttcctaag ccgaaccgcc gtaacgtaag ggtaagcgac gaccctcaga cggtggtggc 4080tcgtcggcgt agagaaagga taagcgagaa gatccggata ttgaagagga tggtgccagg 4140cggtgcaaag atggacactg cctccatgct cgacgaagcc atccgctaca ccaagttctt 4200gaaacggcag gtgaggctag cttcttcagc ctcacactca gcttggagct cctatgtctg 4260acccttcttg cctttgttat taattaccac aactcggata actaattata attctatcac 4320gcgtttcatg ttgatatata tagataaatg gtcgaataag gatttcgatc gaagattgta 4380tggctattga ttacattata tattgtacaa taaatgatgt gtgtatttca attaatgtat 4440gatatatata tatatgtatg cagtatgcat ttatattcta ttctctataa ggaggcaaca 4500ttgccggatt agggctttga tcttatgcaa gttttccgac caaaaatatg aaatacttgt 4560ttggatataa catatgaatc ggataagtgt tactagttat ataactggaa aacaaatgtc 4620tggaataaga attcccggga gaaccaagcc tttctctaat ccctaagatt atagctactg 4680aaacaatgaa acaatgaaga atcagttggg cattagtaaa aaaaaaagaa tcagttgggt 4740tgcttataaa attttgttat aaaatttatg tcgtatgtgt gttagccgta gctgtaaatt 4800tatttccttt gtatgttaat tgtaaaagta aatttattat tcccgatgtt tttaatgatg 4860taaatttaaa tcaagaaaac atattaagtt ctgttaataa atgtattatt tttgtttact 4920aatttccaat aattttttca agaagtacaa ttttttgttt gaaatttcta atttatcatt 4980attgaataat agaacatacc taaaaaccat aaaacatgta ttccttgaaa catttttttt 5040aatataaaaa ttatctttta ggagcaggta aagtactttt ttttataaaa ttgttagtgt 5100atttatgttc gttatagatg tagatatttt gcataataat taataatata tgagatgtgt 5160aatgtatata tgtgagagaa ttgccatatt tattaagcgg tttttgtaaa attgactcaa 5220aatttaaagt caaccacaaa actaacatat gtttttttgg acatttcatt tgccatattc 5280actccacaag ttcagattat ttacgaaaat gtcattaatt ttttttcttt ctgaaatggt 5340atttttactc tctcaacctc atcatcttca agtatttaca agattgtcat tgccatcaat 5400acaccaacca ccatgaacaa ccaatttgaa taatgcacct taaatcgatt tacacttctt 5460ccttctcaat tcttatgaac taaaaacaac atctctttca ctttctctcc atattcatcc 5520aaaaaatcta agatttttat tcaaaaaaat ttatggttca tagaaccatt gaagcttacg 5580attcttggtg tgtcactttt gtttgagatt ataggtgctt ggagaagact tttgtgtgct 5640aaacaagtta tctcaatggt tgaactatga aaatcagtgt ttttttccag atttgttcgc 5700ccaaccgact ttcgggtaag ttttctggct gtagacgact aacatggaag tcttctggtc 5760aatgcacttc tctttgattg tcttcttctg atttacatta tgtagaatga acaagagatg 5820ggaggaacta ggggtaggta aaaaaaccgg aatcgaatta tcaaaccgaa caaacccgaa 5880aaaaccgaac caaatttatg aattatccaa ttggatactg aaattgtata cccgaagaat 5940tagaaccgaa ctcgtaccga accgaaaacc gaataatacc catatagaac cagatgaaaa 6000acctttatac actacttata cgtatatctt tatagtttat attttataca cttgtttttt 6060ttattgatca acgcttgttt tataggagaa ccttgtattg tttggatatt ttatcctcat 6120aatttaagtt ttggtttctg aatgggaaaa tcatttgaat gttattttgg tgttggtttt 6180aattcattta tttgcaagta catcagatta atttggataa tatggttaca aagtaaaaag 6240aaaaaactga acttcaggaa cctaaaaaac cgagaaccga tctgatctga tccgaaattt 6300ttatggttct ttaatggtta ccaatttcaa gaaccgaaat aaacgagaac cgaaagaacc 6360aataaccgaa gaaccgagcg cccactccta ggaggaacca aacaatactt tgatttgaag 6420cccaagataa ttggaaccat gttatattac gcagcttatc tgatgtgggc tttatgtaga 6480actgataatg ggctttaagg cagcctattt cggtccgtca agaagattcc tcctaacgta 6540aggtaggggc aatgagctgc tactctgacc aattaattac gtttcaatag tgatctctca 6600tctgttgtcg gtgtgaaagg aatcgtcttt tgaatttatc ttcatgcgat tcattg 6656706463DNABrassica napus 70aagccagaaa aaaatgtgga agtgtaataa aacaattttc tccaggaaga agatatttca 60gccattcgcc tatttatgtg tatgagatcc cattcaagga catactgttt tccgtgagct 120actgaatgaa tacgatgctt ttgttgtaaa cgcaacctat tggttttttt tttccgccaa 180gttttgtttt tattaatcaa acgggccaag cccaaacggt cgaagcccaa atacactaag 240taccaaacaa aaggcccaca gacacaacgg gctacaacat

taatggctga agcccaagta 300aactaaacgg cccaaaggcc caaacattga aaccggacag cttgacctag gcgcgcgata 360aggagactgg ggtttgaaca cgtgtaagaa tctgggccat tagtatgaca cgcgtcgccc 420gcctcgactt gatcgaaacc tcttcgcaca gccgcatgaa cttgccaccg acgacctcgt 480ttcgccggag ctcagagact gaagagcctc caacgaaacc atagctattc tacgccgctc 540tgtcttcacg ccggagagtt ccatcgatct aacgagatct cctccgacta tgtatcacca 600caaaccctag acaggcgagc cgagaaccac tgatatcctc ctcttgtacg tcctcgccgt 660tgtcggagag tttcatcatc atccgagatc tcatccgcga tggcccacga ccccatacct 720gaccacaaga accacaccgc acttatctca cctcgatcta atcaccacaa cccaaaggac 780atcgggttcc aagacggcag cgcggagctt tgcaagcctc cactctccgt gaccaaaagc 840cggcgaagac ggagctgaat gagcctcccc ttcccggaag ctaaagcggc gacggtggat 900ctgagaaagc ctccacctcc cgcataaaga ccggcgacga tggatctagg gtagcctcca 960tctcccggaa aaaacaccac ctgaacatgc aagccgctcc atctccaccg ggagcctcca 1020attgatcgcg tcgttactcc aaaggccgag ccaccgttga tggatggttg cggtcccaga 1080gctccgacaa ggcgatcgat gacgggactg cgaagaagga gcggagagtg acaactacaa 1140aggaagggaa agagctctgg cgacagcacg gacgctcacg cgccagccgt acgccgaacc 1200caaaagtaga tctggtttct ctcttttctc tctctcgcaa aagaaaacac gcaacctatt 1260gttctccatt ttgaatttga agtgttacat tattaataaa aaggaagaaa gtacaaagct 1320tttcatttag tggttgttgc atatatagta tacttatttg atgggcttat gataagtatg 1380aacatttcac gtaacgggtt tattttgtcg tcaacacatt accggcaatg tgaaaagaag 1440gggcgaggta tctccatttc aattcttctc tttatagatt aatcgaatta tttacatatg 1500aaatgagcgt ttatatagaa ttttcgtgtg gaaaacgaca tgtacacggc atcgaaagac 1560caattagtaa tatactttag tggtgattac atgtttactt atccaattga gaatttaaag 1620catcgacaat accttaatgt cgattaagcc gtccccactt catgtaatga gttatggggg 1680gagagagagg tcccgaaatt cgtcaaataa aacaacttag aactaaaaac cgacaccaag 1740tatcataaag gaaacgttga agaagtcatt tatcgtatcc agctcacaat tcctaagatt 1800aaatcatgac cgttggaaga gcttataaga ttaaattgaa gaaattgtgg gttttagaag 1860aaagacaaga aagagaagaa catgatctta cattgcctat tttggtgtat aggagttgtc 1920aaaaagagga gagagaggag acaattaggt caaataaatg agcactaaaa atggagacat 1980gtgttgagta actattacaa gagcgactta tgcttctata tggcaatgat atcatcacca 2040aagtgcaatg cccctttttg ccctagtttc gtaaagtctc tttccttctt catccttagg 2100aaaaacccta aattaaatcc tgtgttcttg atcttgcttt ttgagtaacc atgattttga 2160ccacacacta gttcttctat attttgtggt ctataggatt ttgctttata tgtgtttctt 2220gtattgcacc gtacgtgcgt atataaattt aaatggttac aacaaggttt attataaaca 2280ggcacaaatt agtccatgag gttatttagc ttgcacaaga ggtatataaa tgtgtgtgtg 2340tgtgttaagt atttaaatat ataaatttgc tacaaaactt aattaacttt atctgattat 2400attttcttta gtgttcttca tttgccaacg ttgaggtagc tattattatt attattatta 2460ttattattat tattattatt attattatta ttattattat tattattatt attattatta 2520ttattatttt gaacattatg tactatgtac gtagttattt tggctagtta tgattcaaat 2580tcttaatttg gataacactt aacagtattt aaaaaaaaaa tactatttaa aatattctta 2640gtactaaaat aattaagatt taacttgaaa ttgaagtatt cgtgttaaac agaaactaca 2700ataaacaaat gattgcatgt taattttttt ttcgattatc ctatatatca gaataaacac 2760atgattgcat gcaaattttg tttttgatta tgtcatcttt tgtttatttt agttttgatg 2820ctaattaata ttttttttat taacaacttc catacattct acctgattct aggtcagata 2880atgacacagc gcaacaaaat tatacaaaac tttcggaaag tagattaccg cggaagtaac 2940atttttgggt agacatacga agtagtttga atcttcaaaa atcatgccat acataaccac 3000ggatcatagt cgacacctca acgtgaagca aatttgacaa tctacataca taaccaacaa 3060aaagtagaat accgtgaaaa cctaaaccca aaatatgatg taaaactcaa gcttggtcca 3120gagcataaaa aaattaaagc catcgctttg gtatcacata tttaaacgtc agtttttttt 3180tggggaagta atataaaaat ataattaaca agaaaattta tgaaataatt agcatgtaaa 3240acactagtct tttggttgtg acaaaacgtt ttcacaaatg ttctataaat aaattcaagc 3300acattttatc tgcaaaatat atactttcac tcataaaata agagcgttta aaacattcat 3360atacgcacta cattgacatg acaaaagaaa tccgcaaata caaacatatt tagttcggat 3420atatctagga aataagacta tattatatat ataaagaaat tagaaaaaaa gaaaattggt 3480atgtataaaa gaaaggtcta tgcgtctcta gtccaaaaac tctatatgtc tggttcaaaa 3540gcagatgcag cagccatagc cccaatagtc atgatggagc ctcatcatct ccttatgaac 3600tggaacaaac ctattgatct cattacacaa gaaaactctt ttaaccacaa tcctcatttc 3660atggtagatc caccttccga aaccctaagc cacttccagc ccccgccgac agtcttctcc 3720gatcccggag gaggagagga agcagaagac gaagaaggag aggaagagat agatgagatg 3780aaggagatgc aatacgcgat tgctgccatg cagcccgtag acatcgatcc agccaccgtt 3840cctaagccga accgccgtaa cgtaagggta agcgaggacc cccagacggt ggtggctcgt 3900cggcgtagag aaaggataag cgagaagatc cggatattga agaggatggt gccaggcggt 3960gcaaagatgg acactgcctc catgcttgac gaagccatcc gctacaccaa gttcttgaaa 4020cggcaggtga ggcttcttca gcctcacact cagcttgggg ctcctatgtc tgacccttct 4080cgcctttgtt attaccacaa ctcggatacc taattataat tctatcacgc gtttcatgtt 4140gatatatata gataaatggt tgaataagga tttcgatcga agattgtatg gctattgatt 4200acattatata ttgtacaata aatgatgtgt gtatttctat taatgtatat atgatatata 4260tctgtttgca gtatgcattt atattctatt ctttataggg aggcaacatg ccggattagg 4320gctttgatcg tatgcaagtt ttccgaccaa aaatatgaaa tacttgtttg gatataacat 4380atgaatcgga taagtgttac tagttatata actggaaaaa aattgtttgg tataagaatt 4440cccgggagaa ccaagccttt ctctaatccc taagatcata gctactgaaa taatgaaaaa 4500aaacaaaaaa aaaacaatga agaatcagtt gggcattagt ccaaaaaaaa aaaagaatca 4560gttggattgc ttataaaatt ttgttataaa atttatgtcg tatgtgtgtt agccgtagct 4620gtaaatttat ttcctttgta tgttaattgt aaaatttaat ttattattcc cgatgttttt 4680aatgatgtaa gtttacatca agaaaacata ttaagttctg ttaataaatg tattattttt 4740gtttactaat ttccaataat tttttcaaga agtacaattt tttgtttgaa atttctaatt 4800tatcaacata cctaaaaacc ataaacatgt attttttgaa acattttttt aatataaaaa 4860ttatttttta ggagcaggtg aagtatttct tttaataaaa ttgttagtgt atttacgttg 4920gttatagttg tagatatttt gcataataat taataatata taagatgtgt aatgtatata 4980tgtgagagaa ataccatatt tactaagagt ttttcgcaat attgactcaa aattcaaagt 5040caatcacaaa actaacatat gtttcttttt agacatttcc tttgccttat tcactccacg 5100ggttcagatt attcacgaaa atgtcattaa attttttttc ttttcgaaaa tggcattttt 5160actctctcaa tttcatcctc ttcaagtatt tacaagattt ccattgccat caatacacca 5220accacaatga acaaccaatt tgaagctctt aatgcacctc aaatcgattt acacttcttc 5280tttttcaatt cttatgaact aaaaacaaca tctctttcaa tttctcccca tattcatcca 5340aaaaacccaa gatttttatt ctaaaatttt aatggttcat agagccattg aagcttacga 5400ttcttggtgg gtcacttttg ttcgagattt taggtacttg gagaagactt ttgtatgcta 5460aacaagttat ctcattggct gaaactatga aatcagtttt ttttccagat ctgttcgccc 5520ggacgacttc cggataagtc ttctggctgt agacgactta catcgaagta ttctggttaa 5580tgcagatgtt agttttgcaa ttgactttta agatgcttac tcgaagatta gtactcttct 5640gttcttgtac cttatgagca ttccaatagt attatatttt tcattctaaa atatatttca 5700atgatatttt acttttcact ctataataga gtgaaaatga gtttacttta taatccccta 5760tatattattt gtgaaacact acaacttctt tttgtagcca catgtcatca ctaggatgat 5820ttttagaatt attagagaaa taggttggtc catctaatta tataataagt tttttattaa 5880attaaccata aattcattat taatgtcatt tattatttcc ttaaataaag attacggaat 5940tgcctaatgt gggtaaagta tatatgacaa ttaatgatct tgaataataa agatatgata 6000aaaaaaatgt atcttctatc aaatttgttt aatttaaaac tattaaaata atttttaaaa 6060aaacacaata accatattat aaaaatttag atttttctgt atatttttat attttgaact 6120ttaaaaaatg acgataaatt actaaaactg ttaaaagtct cacattcaaa ttttgctatc 6180catgttttaa aatttttgtt atgacaaaat acaaatgatt acaaaatcat ataagtaaaa 6240gtctaattta attaatcatt aagatttaaa atatatatgt atatatatca ttctaaatta 6300aactataaac catattgaat aaataaatat tttagtttca aaattttact ttgaataatt 6360ttttttgata aaagttttga actaacattg ataaatgttt ttaaactatc aattaccaaa 6420attattgatc ccacaatgaa aattttgtta tcagtaattt aaa 6463713100DNABrassica napus 71attatatatg gaccaaaaca tgaatgattt ttcggaatca gatactccat tcagcttcga 60acagttatta tatggagatg attggcagag gtgggacact tatccgagcg ttgatgttaa 120tgccaactca atagagccta atcattcgat ggtcgagatg aaccatgatg gtatgacaat 180agatgctact ccaatttcaa tgatatatcc aatggtaata atctactttc tgcatgtttt 240ttttagagat attaaagtat ggataaataa tataagactt attgaaaatg tataggaaga 300aacattcgag cagtggttac agacactggc caatggatca tttgaaatac catcatatga 360agaactcaac ttcacgtatg aggtatggtg aaagataata tatataaagg tatattttat 420gtaacatata atcaatttat tggtaactta tttggttata ttgcatttgt acattttttt 480ctcagtggat atgcatttga atcatagttg aagaaaacaa aaaggaatat atatgctgca 540attggattgt tgatttttaa aatgatatga cgtccaatga cttatttcat tatttccata 600aatttttaca gtatttaaca tgaatagaat gaaatactca ttccatcaat tccacaaaca 660ttccaaaaaa tatacaaaaa atacattcat atgcaatttt aattatgagt gaatagaatg 720aaaatatttc ttttttaact aattccattc atcttattcc atctattttg attccactaa 780tttcatttca ttgttaccat tgacaaccat aatgtgcatg gttgtaaatt tagaactctg 840taaccatata tatatatatt caaaagttta ttagtgtatt tttttctagg aattattgct 900catgagtgag cagataggag atgtctgcat tggtgtagac gttgatatca ttgaggggaa 960tctgaaacga agaaagtatg aagatcgttc tggtcaagcg gagaagtgtg tgatatgtct 1020cgatgaactt aagtgcaatg atgaggcttc tacactggca tgtgggcatg attttcacta 1080cgagtgtatc aaaaactggc taatggtcaa gaacaagtgt cctctttgca agcaacaagc 1140tctttaattg tttcatgatg ctgaatattt gaatgtgtta gtattgctaa cattggtttc 1200ttttgttcaa gtatcggtat tcggtagttt aataaaaaca aatatggaag tttcataaca 1260aaaaaaaaag tctaccaaaa tttatgcaag ttaattatca ttcaagacaa cacccactgc 1320aatttgtcaa aaatggtgaa actcttgcaa gaccttacct gtgaagtgat tgtccttttt 1380catgtttaat aataataatt aattacagta ttatttatga ctttcagaaa actctatcta 1440tcaaccgttc tttactgatt tttccaccca tcactgctct attttttatt tatggcatcg 1500ggaaaaaaag agaagaaaaa gaaatattat atgcaatctt tttttttttt tatgcaatct 1560taccgggtgt taatacttat aactacattt agtgataaat ctgctttgtt aaatccagta 1620caaaatcaat tattgaattt atagactata aagttgtgaa caaaatacaa attatcacta 1680atgggaatat tcatatacag tataataacg taagccacca gattatggct agagtgattt 1740gccacgcgcc tgcctattta ttatgaaaag cctcagtaac tttgtgatga gaagaattca 1800cagagagaga gaggagagag atgggtaatt ccgacgaagg tgatcgtctt cctgctccat 1860cttcttcgga cgaactctcg agcattctcc ggcaggtact gtcccgtgct cccacagctc 1920aaccttcttt ctcaccgaag aaaatcgttt cctccgctga gatgttcaac cgaaccttcc 1980ccctcgttcc cggcggagcg gtttcttacg ccgcttgtgc agccgctgaa actggggaag 2040gcaaatgtgg tttcgaaaac aaggtaaact taacgatgtt agtttccgtg aaactaccct 2100cgcttttgat taaagaaaaa ataacttagt tgttgttttt gcttggtaat ggttaacaca 2160acactaaaga gaaatggagc tagacagcga aattcattga agagaaacat tgatgcacag 2220ttccacaact tgtctgaaaa ggtttcgtct ttatcctttt aagattcttg atttggttta 2280aaaaaaaact agagataata atagaaactg gatatatgca gaggaggagg agcaagatca 2340atgagaaaat gaaagctttg cagaaactga tacccaattc caacaaggtg gtaaatacca 2400agttcgcctt tttatccttc agaactcagt tgtgagaacc atttgttttg ttttttatgt 2460tttgtagaca gataaggcct caatgcttga tgaagctata gaatatctga aacagcttca 2520acttcagttt caggttcttt ttctatatgt tccttacgct atgatcatga acaactaaat 2580ttgtaaaacc aaacatctga ttaacttaat gaatgcagac gttagccgct atgaatggtt 2640taggcctaaa tcctctgcga ttaccaccaa ttctaccgcc tacgcagaca aggatcactg 2700gaacctcaga acaagggctg aaccttgaga ctctgcttgg tggttctcac tcgatggcta 2760accgtgaacc acccgaaact caggaaatgt gctttcccac aaccactctg ctttgaagac 2820aacgttcaga cagtgaagag gatccgaagt cagatttcct ctccacagaa acatgagtcg 2880aaaatgattt gtagagtcta gtatttggtt atataagtgt tatgctaata agctttagga 2940ctgaacaaaa ctgagtagct tcattataat gatgtcagaa taaatttaac tacatgatta 3000agatcaaatg gctatgtctg tgagttgcag ccatccgtac gcacaccggt ctcggatact 3060tcgagccatg gatgctgctg actgaaccgc aggtcttatc 3100723168DNABrassica napus 72atatggacca aaacatgaat gatttttcgg aatcagatac tccattcagc ttcgaacagt 60tattatatgg agatgattgg cagaggtggg acacttatcc gagcgttgat gttaatgcca 120actcaataga gcctaatcat tcgatggtcg agatgaacca tgatggtatg acaatagatg 180ctactccaat ttcaatgata tatccaatgg taataatcta ctttctgcat gtttttttta 240gagatattaa agtatggata aataatataa gacttattga aaatgtatag gaagaaacat 300tcgagcagtg gttacagaca ctggccaatg gatcatttga aataccatca tatgaagaac 360tcaacttcac gtatgaggta tggtgaaaga taatatatat aaaggtatat tttatgtaac 420atataatcaa tttattggta acttatttgg ttatattgca tttgtacatt tttttctcag 480tggatatgca tttgaatcat agttgaagaa aactaaaagg aatatatatg ctgcaattgg 540attgttgatt tttaaaatga tatgacgtcc aatgacttat ttcattattt ccataaattt 600ttacagtatt taacatgaat agaatgaaat actcattcca tcaattccac aaacattcca 660aaaaatatac aaaaaataca ttcatatgca attttaatta tgagtgaata gaatgaaaat 720atttcttttt taactaattc cattcatctt attccatcta ttttgattcc actaatttca 780tttcattgtt accattgaca accataatgt gcatggttgt aaatttagaa ctctgtaacc 840atatatatat atattcaaaa gtttattagt gtattttttt ctaggaatta ttgctcatta 900gtgagcagat aggagatgtc tgcattggtg tagacgttga tatcattgag gggaatctga 960aacgaagaaa gtatgaagat cgttctggtc aagcggagaa gtgtgtgata tgtctcgatg 1020aacttaagtg caatgatgag gcttctacac tggcatgtgg gcatgatttt cactacgagt 1080gtatcaaaaa ctggctaatg gtcaagaaca agtgtcctct ttgcaagcaa caagctcttt 1140aattgtttca tgatgctgaa tatttgaatg tgttagtatt gctaacattg gtttcttttg 1200ttcaagtatc ggtattcggt agtttaataa aaacaaatat ggaagtttca taacaaaaaa 1260aaaagtctac caaaatttat gcaagttaat tatcattcaa gacaacaccc actgcaattt 1320gtcaaaaatg gtgaaactct tgcaagacct tacctgtgaa gtgattgtcc tttttcatgt 1380ttaataataa taattaatta cagtattatt tatgactttc agaaaactct atctatcaac 1440cgttctttac tgatttttcc acccatcact gctctatttt ttatttatgg catcgggaaa 1500aaaagagaag aaaaagaaat attatatgca atcttttttt tttttttttt atgcaatctt 1560accgggtgtt aatacttata actacattta gtgataaatc tgctttgtta aatccagtac 1620aaaatcaatt attgaattta tagactataa agttgtgaac aaaatacaaa ttatcactaa 1680tgggaatatt catatacagt ataataacgt aagccaccag attatggcta gagtgatttg 1740ccacgcgcct gcctatttat tatgaaaagc ctcagtaact ctgtgacgag aagaattcac 1800agagagagag aggagagaga tgggtaattc cgacgaaggt ggtcgtcttc ctgctccatc 1860ttcttcagac gaactctcga gcattctgcg gcaggtactg tcccgtactc ccacagctca 1920accttctttc tcaccgaaga aaatcgtttc ctccgctgag atgttcaacc gaacattccc 1980cctcgttccc ggcggagcgg tttcttacgc cgcttgtgca gccgctgaaa ctggggaagg 2040caaatgtggt ttcgaaaaca aggtaaactt aacgatgtta gttgccgtga aactaccctc 2100cttttgatta aagaaaaaaa ataacttagt tgttgttttt gcttggtaat ggttaacaca 2160acactaaaga gaaatggagc taaacagcga aattcattga agagaaacat tgatgcacag 2220tttcacaact tgtctgaaaa ggtttttgtc tttatccttt ttaagattct tgatttggta 2280aaaaaaaaac tagagataat aatagaaact ggatatattg cagaggagga ggagcaagat 2340caatgagaaa atgaaagctt tgcagaaact gatacccaat tccaacaagg tggtaaatac 2400caagttcgcc tttttatcct tcagaactca gttgtgagaa ccatttgttt tgttttttat 2460gttttgtaga cagataaggc ctcaatgctt gatgaagcta tagaatatct gaaacagctt 2520caacttcagt ttcaggttct ttttctatat gttccttacg ctatgatcat gaacaactaa 2580atttgtaaaa ccaaacatct gattaactta atgaatgcag acgttagccg ctatgaatgg 2640tttaggccta aatcctctgc gattaccacc aattctaccg cctacgcaga caaggatcac 2700tggaacctca gaacaagggc tgaaccttga gactctgctt ggtggttctc actcgatggc 2760taaccgtgaa ccacccgaaa ctcaggaaat gtgctttccc acaaccactc tgctttgaag 2820acaacgttca gacagtgaag aggatccgaa gtcagatttc ctctccacag aaacatgagt 2880cgaaaatgat ttgtagagtc tagtatttgg ttatataagt gttatgctaa taagctttag 2940gactgaacaa aactgagtag cttcattata atgatgtcag aataaattta actacatgat 3000taagatcaaa tggctatgtc tgtgagttgc agccatccgt acgcacaccg gtctcggata 3060cttcgagcca tggatgctgc tgactgaacc gcaggtctta tcttcgtcaa tgctttctct 3120acttgaattc tgacaccaac atccatctgc actgattccg gcgccata 3168733168DNABrassica napus 73atatggacca aaacatgaat gatttttcgg aatcagatac tccattcagc ttcgaacagt 60tattatatgg agatgattgg cagaggtggg acacttatcc gagcgttgat gttaatgcca 120actcaataga gcctaatcat tcgatggtcg agatgaacca tgatggtatg acaatagatg 180ctactccaat ttcaatgata tatccaatgg taataatcta ctttctgcat gtttttttta 240gagatattaa agtatggata aataatataa gacttattga aaatgtatag gaagaaacat 300tcgagcagtg gttacagaca ctggccaatg gatcatttga aataccatca tatgaagaac 360tcaacttcac gtatgaggta tggtgaaaga taatatatat aaaggtatat tttatgtaac 420atataatcaa tttattggta acttatttgg ttatattgca tttgtacatt tttttctcag 480tggatatgca tttgaatcat agttgaagaa aactaaaagg aatatatatg ctgcaattgg 540attgttgatt tttaaaatga tatgacgtcc aatgacttat ttcattattt ccataaattt 600ttacagtatt taacatgaat agaatgaaat actcattcca tcaattccac aaacattcca 660aaaaatatac aaaaaataca ttcatatgca attttaatta tgagtgaata gaatgaaaat 720atttcttttt taactaattc cattcatctt attccatcta ttttgattcc actaatttca 780tttcattgtt accattgaca accataatgt gcatggttgt aaatttagaa ctctgtaacc 840atatatatat atattcaaaa gtttattagt gtattttttt ctaggaatta ttgctcatta 900gtgagcagat aggagatgtc tgcattggtg tagacgttga tatcattgag gggaatctga 960aacgaagaaa gtatgaagat cgttctggtc aagcggagaa gtgtgtgata tgtctcgatg 1020aacttaagtg caatgatgag gcttctacac tggcatgtgg gcatgatttt cactacgagt 1080gtatcaaaaa ctggctaatg gtcaagaaca agtgtcctct ttgcaagcaa caagctcttt 1140aattgtttca tgatgctgaa tatttgaatg tgttagtatt gctaacattg gtttcttttg 1200ttcaagtatc ggtattcggt agtttaataa aaacaaatat ggaagtttca taacaaaaaa 1260aaaagtctac caaaatttat gcaagttaat tatcattcaa gacaacaccc actgcaattt 1320gtcaaaaatg gtgaaactct tgcaagacct tacctgtgaa gtgattgtcc tttttcatgt 1380ttaataataa taattaatta cagtattatt tatgactttc agaaaactct atctatcaac 1440cgttctttac tgatttttcc acccatcact gctctatttt ttatttatgg catcgggaaa 1500aaaagagaag aaaaagaaat attatatgca atcttttttt tttttttttt atgcaatctt 1560accgggtgtt aatacttata actacattta gtgataaatc tgctttgtta aatccagtac 1620aaaatcaatt attgaattta tagactataa agttgtgaac aaaatacaaa ttatcactaa 1680tgggaatatt catatacagt ataataacgt aagccaccag attatggcta gagtgatttg 1740ccacgcgcct gcctatttat tatgaaaagc ctcagtaact ctgtgacgag aagaattcac 1800agagagagag aggagagaga tgggtaattc cgacgaaggt ggtcgtcttc ctgctccatc 1860ttcttcagac gaactctcga gcattctgcg gcaggtactg tcccgtactc ccacagctca 1920accttctttc tcaccgaaga aaatcgtttc ctccgctgag atgttcaacc gaacattccc 1980cctcgttccc ggcggagcgg tttcttacgc cgcttgtgca gccgctgaaa ctggggaagg 2040caaatgtggt ttcgaaaaca aggtaaactt aacgatgtta gttgccgtga aactaccctc 2100cttttgatta aagaaaaaaa ataacttagt tgttgttttt gcttggtaat ggttaacaca 2160acactaaaga gaaatggagc taaacagcga aattcattga agagaaacat tgatgcacag 2220tttcacaact tgtctgaaaa ggtttttgtc tttatccttt ttaagattct tgatttggta 2280aaaaaaaaac tagagataat aatagaaact ggatatattg cagaggagga ggagcaagat 2340caatgagaaa atgaaagctt tgcagaaact gatacccaat tccaacaagg tggtaaatac 2400caagttcgcc tttttatcct tcagaactca gttgtgagaa ccatttgttt tgttttttat 2460gttttgtaga

cagataaggc ctcaatgctt gatgaagcta tagaatatct gaaacagctt 2520caacttcagt ttcaggttct ttttctatat gttccttacg ctatgatcat gaacaactaa 2580atttgtaaaa ccaaacatct gattaactta atgaatgcag acgttagccg ctatgaatgg 2640tttaggccta aatcctctgc gattaccacc aattctaccg cctacgcaga caaggatcac 2700tggaacctca gaacaagggc tgaaccttga gactctgctt ggtggttctc actcgatggc 2760taaccgtgaa ccacccgaaa ctcaggaaat gtgctttccc acaaccactc tgctttgaag 2820acaacgttca gacagtgaag aggatccgaa gtcagatttc ctctccacag aaacatgagt 2880cgaaaatgat ttgtagagtc tagtatttgg ttatataagt gttatgctaa taagctttag 2940gactgaacaa aactgagtag cttcattata atgatgtcag aataaattta actacatgat 3000taagatcaaa tggctatgtc tgtgagttgc agccatccgt acgcacaccg gtctcggata 3060cttcgagcca tggatgctgc tgactgaacc gcaggtctta tcttcgtcaa tgctttctct 3120acttgaattc tgacaccaac atccatctgc actgattccg gcgccata 3168747697DNABrassica napus 74acatgaatga tttttcggaa tcagatactc cattcagctt ccaacagtta ttatatggag 60ataattggca gaggtgggac acttatccga gcgttgatgt taatgccaac tcaatagagc 120ctaatcattc gatggttgag atgaaccatg atggtctgac aatagagcct aatccaattt 180caatgatata tccaatggta ataatctact ttctgcatgt ttttttgaga gatattaaag 240tatggatgaa caatataaga cttattgaaa atgtatagga agaaacattc gagcagtggt 300tacagacact ggccaatgga tcatttgaaa taccatcata tgaagaactc aacttcatat 360atgaggtatg gtgaaagata atatatatac atagaaaaca atatatatat atatatatat 420aaaaaaataa aagggtatat tttatgtaac atataatcaa tttattggta acttaattgg 480ttatattgca tttgtacatt ttttcgagtg gatatgcatt tgaatcatag ttgaagaaaa 540ctaaaaggaa tatatatgct gcaattggat tgttgatttt tggaatgata tgacgtggaa 600tgacttattt cattatttcc ataaaaaatt ttcagtattt aacatgaata taataaaata 660ttcattccat caattccaca aacattccaa aaaaaaacat tcatatgcaa ttttagttat 720gaatgaataa aatgaaaata tttctttttt aactaattcc attcatctta ttccatctat 780ttttattcca ttaatttcat ttcattgtta ccattgacaa ccataatgtg catggttgta 840aatttagaac tctgtaacta tatatattca aaaatttatt agtgcatttt tttctaggaa 900ttattgctca ttagtgagca gataggagat gtctgcattg gtttagacgt tgatatcatt 960gaggggaatc tgaaaggaag aaagtatgaa gatcgttctg gtgaagcgga gaagtgtgtg 1020atatgtctcg atgaacttaa gtgcaatgat gaggcttcta cactggcatg tggacatgat 1080tttcactacg agtgtatcaa gaaatggcta atggtcaaga acaaatgtcc tctttgcaag 1140caacaagctc tttaagtgtt tcattatgct gaatatttga atgtgttagt attgccaaca 1200ttggtttctt ttgttcaagt atcggtattc ggtagtttaa tgaatacaaa tatggaagtt 1260tcataacaaa aaaaaaagtc taccgaaatt tatgcaagtt aattatcatt caagacaaca 1320cccactgcaa tttgtcaaaa atggtgaaac tcttgcaaga ccttacctgt gaagtgattg 1380tcctttttca tgtttaataa taataattaa ttacagtatt atttgaaact ctttgtaaac 1440ttaaagtact tttcttattg aatgaaggca acataagagt tgcttaaata acaaagcgta 1500cagatactcc cgacagtttt ggtaaagtct taaccacttc ctatagtaga ggaagactta 1560ttcaaataac ttaaagcaga aaataattca aaactaccaa ctaaagactc taccaactaa 1620agactcgatc ttttttattc attaatccct cccttaaact tgaactcctt tataaagttt 1680aaacttcctt taagctgact cccatgtgtg ctctcagctt ctcaaacgct tcaagtttca 1740ccggctttgt catgatatcc gacaattgct tatgagttga acaatactcc attgagatga 1800tgccttcatt cacaagctct cttaagaaat gaaacctcac atgaatatgc ttgcttcttc 1860catgtagaac aggattcctt gacaacttga tagctgaact attatcacag aaaagaacag 1920ttccatcacc ttgatcaaac ccaatttcag ccaacacgtt cctcatccat acagcttgac 1980aagctccaat tgctgcagca acatactctg cttccgtggt agagagtgtg acaattggtt 2040gtttctttga gctccaggac acagctcctc ctccaaacat gaaagtgtag cctgatgtac 2100tctttctgtc atcctcatcg cctgcataat cactgtccac ataccctaca agcttttctt 2160ctccaccacg caagtactga ataccatagc ttgcagttcc ttgcacatac ctcaaaatac 2220gcttaacagc catcatgtgc ccttcatttg gattctccat gtatctacta accacattca 2280cagagtatat cagatcaggt cgagtagctg ttaagtacct caagcttccc acgagttgct 2340tgtaagcggt tggatcaact tcttctccag ctcctgtctt ggtcagtttg ttccctggaa 2400ccattggatt cttcacagag ttgcagtctt tcattccata cttctttaaa gtctccagtg 2460catacttctt ttggccaata aagatcccaa cctcgtcttg cacaacctcc acgcctagaa 2520agtacttcat ctttcctaga tcagtcattg agaactcttc ttgcattgaa gttttgaagc 2580tctccagcat gctttccgag ttactagtgt aaatgaggtc gtctacgtac aagctcagga 2640tcaacgagtt cccttcttct gtttttacga acaatgtgtg ttcgcaataa catttctcaa 2700agccttcctt tataaagtaa ctctcgatcc tgctatacca ggccctagga gcttgtctta 2760atccatacaa agcctttttc aacttgtaga ccttctcact gtcaacctga gactcgaacc 2820ccttaggctg ctcaacgtaa acgtcctcct taagctcacc atgtaggaac gcgctcttca 2880catctagttg atatacactc catcttcttt gagccgctaa gccgagaatc agcctgatcg 2940tgtcccatct ggccacaggt gcaaaaactt catggaagtc tactccatag gtttggtgaa 3000agccctttgc caccagtctc gctttgtact tatccacctc tcctttttca ttcaacttgg 3060tcttgaagac ccatttcact cctatcacct tcacattggc aggtagacta accagctccc 3120aagtgttgtt atcctctatt gattcaatct cagtttccat tgcctttctc catttgtcat 3180gttggatagc atcttcaaac ttctctggat cctcatctga caagaacagc gcaactagtt 3240cttctccatc atcttccatg ataagacccc tcccatcaac cacgtaatct ttcatccaga 3300ctggtcttag tttgcttctt cctgctctgc ttgtgtctga tctttcttct ggtgtagaga 3360cagttctttc tccatcagac tcagtgttat ctcctccttc tgtctcttcg ttagttgctg 3420gtgctgtcac caggttctca ccctgattcc tctcttcctc ttcttcagct tctccacatt 3480cgacaaattt gtcttcatca atgttatctt cccagttcca ccgcgtttct tcgtcaaacc 3540tcacgtcttt gcttattatg atcctcttcc caataggatc ataaagtctg taagccttcg 3600actccttgct tacaccaaac atgacacatc tcacactttt ctcatccagc ttcacacgct 3660tctcataagg tataagtgca taagccacac agccaaacac acggagatgc tctacagacg 3720gcttgtgctt actccacttc tcacttggcg tcacttctcc caatatcttt gttggacttc 3780tgttaagtat atggacagca tactgtgttg cttctgtcca atacttgatc ggcaccttca 3840taccaaacag catgcacctg accatattca gaatgcttct attcttcctc tctgccacgc 3900cgttctgctg aggcgtgtat gcggctgtca gctgtcgttt gatgccgtgc tccacacaat 3960agtcctgaaa cgcctttgaa ttgtattctc cacctctgtc ggttcttaga cagacgagca 4020gctcaccact ctccctctca gcggcatttt tgaactcctt gaaagttcta agtgcctctg 4080atttctctgc tagaaagaag ctccaacact ttcggctaaa ctcatcaatg aaattgatta 4140tgtacctctt gccgctttca gatgtgggtg atattggccc gcagatgtca ctgtgaacta 4200gctccagtcg ttgtgttgct ctccatagac tcttctttgg tatcttttct ctgatctgct 4260tccccttcat acacacctca catactgtcc cgttggtgtt gatctttggt agaccttcca 4320ccatcgcctt ttctgctagt gttagcaaac tgttgtggtt caaatgtccg aagcgtttgt 4380gccaagtttc ctccattgtc tccatcgtat tctgaacttg gtttgcctcc aacgcttcct 4440taggctccct gacaatagca gttactacaa acatcctgtt tgtggtcatc agagaatgca 4500tgatcagtct ccgttgttgt ttgtgccaga tctcacacgt atctccttca attataatcc 4560tcaacccctt ctgttgcaac tgaccgacag acagtagatt gttcttcaag ccaggcacaa 4620agtatactgc agtgatcact tgagtagtac cattgatctc caatcttagg ttacctcttc 4680cctcaacttg cattcgccta tcatctccaa gctttacttg ttgtctgaac tggctatcaa 4740actctgaaaa ccactccttg gttccacaca tgtggttgct acaccctgag tcaaggtacc 4800acacgtgtct ttcttcagtc tcaacttgag tcatcagcaa aatatcctcc tctaactctg 4860catagttagc tgccttctcc cagctaggac attcgttttt gaaatgcccc atcttgtggc 4920acttaaagca ctcaatgttg ctcttgtcaa agcttgtatt tcctctgtct tgtcctccac 4980gacctcctct gtaactgcct cttcctctgg tttgatatcc tcctcgtcca ttggagtaac 5040ctgagtagcc acctcgtccc cttcctcctt ccatcttcag gacttgttcc tctcctgtgt 5100gtttactcag gttctgctca tgtacgagta atgagctttg taactcatcc accgacagct 5160ccttgatatc ctttgattcc tctatcgcac ataccacata tgtgaacttc tccacaaggg 5220ttcttaggat cttctcaacc accttttcat ctggcatgtc ttctccacgg ttccgcatgt 5280cattagcaac caccatgact ctagagaaat agtctgcgat tgagtcacct tccttcatct 5340ccaggacttc aaagtttctt ctcagcctct gtaattgcgc actttgaacc ctcttgttgc 5400cttggtactt tgccttcagc gactcccata tgtcttttgc ggtgtccttc ttcacaatcg 5460tcttcagaac agtcttgtcg atggaggcaa agagatagtt cttcaccttg aagtctttga 5520gcttttgctc cgcaagctcg gttctctgag cttcagacag tgtcacacta ctttcatgtt 5580gaatgattcc ggtattgatc agttcccacc actccttgga tctcaacaaa ttctccataa 5640gcattgccca atgctcatag tctccatcaa gctttggtat gctcaacgag gactcctttt 5700cactcatctc tcttactttc ggattaccta gaactcacaa cagtttttgt tagagctctg 5760ataccacttg aaactctttg taaacttaaa gtatttttct tattgaatga aggcaacata 5820agagttgctt aaataacaaa gcgtacagat actcccgaca gttttggtaa agtcttaacc 5880acttcctata gtagaggaag acttattcaa ataacttaaa gcagaaaata attcaaaact 5940accaactaaa gactctacca actaaagact cgatcttttt tattcattat tatttatgac 6000tttcagaaaa ctctatctat caaccgttct ttactgattt ttccacccat cactgctcta 6060ttttttattt atttatggca tcgggaaaaa aagagaagaa aaagaaaaat tatatgcaat 6120tttaccgggt gttaaaactt ataattacat ttggtgataa atctgctttg ttaaatccag 6180tacaaaatca attattgaat tcatagacta taaagttgtg aaccaaatac aaattatcac 6240taatgggaat attcatatac agtatagtaa cgtaagccac cagattatgt ctagagtgat 6300ttgccacgcg cctgcctatt tattatgaaa agcctcagta acttgtgatg agaagaattc 6360acagagagag aggagagaga tgggtaattc cgatgaaggt gatcgtcttc ctgctccatc 6420ttcttcggac gaactctcga gcattctccg gcaggtactg tcccgtactc ccacagctca 6480accttctttc tcaccgaaga aaatcgtttc ctccgctgag atgttcaacc gaaccttccc 6540cctcgttccc ggcggagcgg tttcttacgc cgcttgtgca gtcgctgaaa ctggggaagg 6600caaatgtggt ttcgaaaaca aggtaaactt aacgatgtta gttgccgtga aactaccctc 6660gcttgttgat taaagaaaaa aataacttta ttgtgttttt ggttggtaat ggttaaaaaa 6720cactaaagag aaatggagct agacagcgaa attcattgaa gagaaacatt gatgcacagt 6780tccacacctt gtctgaaaag gttttgtctt tatcctttta agattcttga tttggtttaa 6840aaaaaaaact agagataata atagaaactg gatatattgc agaggaggag gagcaagatc 6900aacgagaaaa tgaaagcttt gcagaaactg atacccaatt ccaacaaggt ggtaaatacc 6960aagttcgact ttttatcttt cagaactcag ttgtgagata catttgtttt ggtttttttt 7020tatgttttgt agacagataa ggcctcaatg cttgatgaag ctatagaata tctgaaacag 7080cttcaacttc agtttcaggt tctttttcta tatgttcctt acgctatgat cataaacaac 7140taaatttgta aacccaaaca tctgattaac ttgatgaatg cagacgttag ccgctatgaa 7200tggtttaggc ctaaatcctc tgcgattacc accaattcta ccgcctacgc agacagggat 7260cactggaacc tcagaacaag ggctgaacct tgagactctg cttggtggtt ctcactcgat 7320ggctaacctt gaaccacccg aaccaactca ggaaatgtgc tttcccacaa ccactctgct 7380ttgaagacaa cgttcagaca gtgaagagga ttcgaagtca gatttcctct ccacagaaac 7440atgagccgaa aatgatttgg ttatatttca aagtgttatg ctaataagct ttaggactga 7500acaaaactgc tactggttat atttcaaagt gttatgctaa taagctttag gactgaacaa 7560aactgagtag cttcattata atgatgtaag aaaaaaaatg tataaattta actacatgat 7620tacgatcaaa tggctatgtc tgtgagttgc agccatccgt acgcacaccg gtctcggata 7680cttcgagcca tggatgc 7697757512DNABrassica napus 75aacaaaaatg atagatggtg cactaataat taatttatag tcagtttaat aaaaagctta 60ttatatattt agatgaacca acatattttt ctaatgatta taagaatcat catggtgatg 120acctaaaaag ttgtaatgct cttcttttaa catatagggg atttcacatc tgattcttta 180ttataagctc tattcttgtt tggaaaatat aatatacata ttaaggaaat tcgaggggtt 240taattttgtt gtgatttatt agaattaatt tttatggaac tttttaacta atttattatg 300tgagaataca tatggtgttg atacgtcaac tttttttttt gtgagaaata ttattgtatt 360gacacttcaa ctttttttgt gagaaacatt ttggtgacga cacatcaaca aggctctctc 420aatgattctc aaataatata tagaagattt taaggagaaa ataggaatat aaatagtaat 480ttcataaatt tgagagcata ctattcacaa tcttatttta tttttgaaca ttgttattta 540taaactgatc cttaaatttt atgtgttttg ttattaatac aattataagt tcatataaat 600cattgtatat tccgatggaa ttaaagttta gggtttaaaa acacgtgcaa aataggttac 660acacgattcg ttaaagatcc acgagcacta caataagaca taaaaacttt atatggtata 720acatatgcta actaatcgaa tattacaata tttgtatacg gtaaataaaa aatcaactta 780caaagacgat ttcgggaatt ctcatagtat tttcaaatct attttaccag tgattttgaa 840gcagttcaaa catcataaac ctcgttaaat cattgtataa tgcatttatc gttgatatgc 900ttttctttgg atttggtaac cttaactcca aatcgttaga aatccgtcaa gaatttgtca 960agaacctgtt gagaatttat atctgattct gacaaatcgg ggtttagggt cttgtttaac 1020gaaaacaaat tcttgatggc ttcttgatgg atgtgtattt catcagaaaa ctaaattttt 1080acccataaaa caaagcacca atattttggc aacctcctaa agtgaatatt tctttacaga 1140ttcccgacaa catatcattt gctcgataat ccgttacgaa tctgaaaatt tacccataaa 1200aagaaaacta aatttttcta tacataattt agccgatttt tttcttcaat atttccattt 1260gatttattct ctcaaaatac attaaactcc tctttatcct caaaatcata atcgaaatca 1320catgttaagt tggttgcttt aggaattaga atattttgtt agattttttc aatgttaagt 1380taaataagtt gttggtttta agattgttaa ttttagaaat ttagatgttt gttttaccga 1440tttagatgtt agttttattt ttgaatgtta atttagggtt tgcatgttag ttttacgatg 1500tagttgttaa ttttaggatt tgtagtttaa aatttttttt ttacgttcgc gtaacattaa 1560aaatattttt catactgaaa acatattttt taattttaaa gttattatat tatttaaaag 1620taattctaaa catttattat atatatttac taatttatga atgtttttac aaaaaaaaat 1680taatttacaa ttgatttttt attattttat attttatatg tatttttatt ataaataatc 1740tttacaattt gtttttgtat tttacatatc ttcaaacact ttattattta aagatgattt 1800tatttaattt tattaaaaat atttgattta tatattctta ttaaaaatat taatttactg 1860tttattattt tatataattt tataaatcat tttatttaga gtttaattta tttacgattt 1920attgttttaa tttgttaggc aaattagtct aacaaattaa acaaaaaaaa atataaataa 1980attaaatttt aaacaaaatg ttttataaaa atatttatat ccttataagg atataaacta 2040taattttgtg ttatttttat atttaaacta ttattatatt taattatttt taaataaatt 2100tatttatatt tttgtatatt tttattaaaa ctaatatttt atttaattat gaaaattgaa 2160aacgtttttt tggttatttt tgaaaatttt gacaaggttg aaaaatcgct ggaaatattt 2220ttgaggaatt ttagacaaaa aaatatcgat gataaacaat tggttggaat ccgtcaagaa 2280tttttgacaa aataaactcg aaatttgttt tcactgggaa ttagttattt tcttgtattt 2340gcattatcta ataaacaatg caaccataaa caacaaatga aataaagttt taattgacaa 2400atcttttaga agagtgaaac gttaatggtg gacgaagccc ccacctcaat tgacaataaa 2460aagagtggaa aagagggaag atggcagaga gaaaagtcca aagagaggaa atcatgaaga 2520gaagaagaca atgtaaccta cttatttgcc tcgatgaaga tagaggagaa gatagaggtg 2580gaatatatat agaagagaga aagatttggg aggaaacccg attgagcata tatcagatct 2640agaatggatt ggattagata actcgaactt atttggttaa tctttttagt ttagggaagg 2700ttatgaaaaa ttacctctga tctaattttt aaatttttta tcttatatac attgtgcccc 2760ccgactaaca atatatctag atccatccct gattaggtcc atgagatata tcatattttt 2820tatttttctt atttaattaa ttatctataa tatttgttga tatatttaaa attttacata 2880tcgttgaaag ccagaaaaat atgtggaagt gtaataaaac aattttctcc aggaagaaga 2940tatttcagcc attcgcccat ctatgtgtat gagatcccat tcaaggccat attctaagat 3000tgactttatc taatttactg tattttccgt gggctactga atgaatacga tgcttttgtt 3060gtaaaacgca acctattgtt ctccattttg aatttgaagt gttacattgt tgataaaaag 3120gaagaaagta caaagctttt catttagtgg ttgttgcata tatatactta tttgatgggt 3180ttatgataag tatgaacatt tcacgtaacg ggtttatttt gtcgtcaaca cattatcggc 3240aatgtgaaaa gaaggggtga ggtatctcca tttcaattct tctctttata tattaatcga 3300attatttacg tatgaaatga acgtttatat agaaatttcg tgtggaaaac gacatgtaca 3360cggcatctca agaccaatta gtaatatact ttagtggtga ttacatgttt acttatccaa 3420ttgagaattt aaagcatcga caatacctta atgtcgatta agccgtcccc acttcatgta 3480atgagttatg gggggagaga gagatcccga aattcgtcaa ataaaacaac ttagaactaa 3540aaaccgacac caagtatcat aaaggaaatg ttgaagaagt catttatcgt atccagctca 3600caattcctaa gattaaatca tgaccgttgg aagagcttat aagattaaac tgaagaaatt 3660gtgggtttta gaagaaagac aagaaagaga agaacatgat cttacattgc ctattttggt 3720gtataggagt tgtcaaaaag aggagagaga ggagacaatt aggtcaaata aatgagcact 3780aaaaatggag acatgtgttg agtaactatt acaagagcga cttatgcttc tagaaccaag 3840cctttctcta atccctaaga ttatagctac tgaaacaatg aaacaatgaa gaatcagttg 3900ggcattagta aaaaaaaaag aatcagttgg gttgcttata aaattttgtt ataaaattta 3960tgtcgtatgt gtgttagccg tagctgtaaa tttatttcct ttgtatgtta attgtaaaag 4020taaatttatt attcccgatg tttttaatga tgtaaattta aatcaagaaa acatattaag 4080ttctgttaat aaatgtatta tttttgttta ctaatttcca ataatttttt caagaagtac 4140aattttttgt ttgaaatttc taatttatca ttattgaata atagaacata cctaaaaacc 4200ataaaacatg tattccttga aacatttttt ttaatataaa aattatcttt taggagcagg 4260taaagtactt ttttttataa aattgttagt gtatttatgt tcgttataga tgtagatatt 4320ttgcataata attaataata tatgagatgt gtaatgtata tatgtgagag aattgccata 4380tttattaagc ggtttttgta aaattgactc aaaatttaaa gtcaaccaca aaactaacat 4440atgttttttt ggacatttca tttgccatat tcactccaca agttcagatt atttacgaaa 4500atgtcattaa ttttttttct ttctgaaatg gtatttttac tctctcaacc tcatcatctt 4560caagtattta caagattgtc attgccatca atacaccaac caccatgaac aaccaatttg 4620aataatgcac cttaaatcga tttacacttc ttccttctca attcttatga actaaaaaca 4680acatctcttt cactttctct ccatattcat ccaaaaaatc taagattttt attcaaaaaa 4740atttatggtt catagaacca ttgaagctta cgattcttgg tgtgtcactt ttgtttgaga 4800ttataggtgc ttggagaaga cttttgtgtg ctaaacaagt tatctcaatg gttgaactat 4860gaaaatcagt gtttttttcc agatttgttc gcccaaccga ctttcgggta agttttctgg 4920ctgtagacga ctaacatgga agtcttctgg tcaatgcact tctctttgat tgtcttcttc 4980tgatttacat tatgtagaat gaacaagaga tgggaggaac taggggtagg taaaaaaacc 5040ggaatcgaat tatcaaaccg aacaaacccg aaaaaaccga accaaattta tgaattatcc 5100aattggatac tgaaattgta tacccgaaga attagaaccg aactcgtacc gaaccgaaaa 5160ccgaataata cccatataga accagatgaa aaacctttat acactactta tacgtatatc 5220tttatagttt atattttata cacttgtttt ttttattgat caacgcttgt tttataggag 5280aaccttgtat tgtttggata ttttatcctc ataatttaag ttttggtttc tgaatgggaa 5340aatcatttga atgttatttt ggtgttggtt ttaattcatt tatttgcaag tacatcagat 5400taatttggat aatatggtta caaagtaaaa agaaaaaact gaacttcagg aacctaaaaa 5460accgagaacc gatctgatct gatccgaaat ttttatggtt ctttaatggt taccaatttc 5520aagaaccgaa ataaacgaga accgaaagaa ccaataaccg aagaaccgag cgcccactcc 5580taggaggaac caaacaatac tttgatttga agcccaagat aattggaacc atgttatatt 5640acgcagctta tctgatgtgg gctttatgta gaactgataa tgggctttaa ggcagcctat 5700ttcggtccgt caagaagatt cctcctaacg taaggtaggg gcaatgagct gctactctga 5760ccaattaatt acgtttcaat agtgatctct catctgttgt cggtgtgaaa ggaatcgtct 5820tttgaattta tcttcatgcg attcattgtt ttataactac cacacatgtg acataactaa 5880tagtctcgaa tcaggacatc aaaatttatt tttttgtttg tggcaatgat tcaaatcgac 5940ggcatagcat actgattaaa aatatataca atacactggt tattaaaacg ttatagttgt 6000ctagatttta ataacaggtg ataatagatc aagtatatag taaatgtttt atctcaccca 6060ttacatattt catattaaac atttcagatt tatttgtaaa tggattcaac tgcctgttac 6120ccttcactcc ttttgattaa catcgcgtcg caagtcacaa ccaccattta gttggggcga 6180ttcgtatatt ttgggagtat ataataacat acataactat caaaccaact ggtgtcgacc 6240tttggtgtta actaatttga tttaactctt ttttggaata aataaaataa aatttcagag 6300cagctgttca atagtaaaat gacaggtggg atctagggtt tgtggtcctt acgatcgatt 6360gattactact acttaacttg gcattatttg gagtcacttt tgcttattaa taattagatt 6420ttgtttacaa acgtgtggtg atagtctggg acaagttgac ctgttgacac ccaatcattt 6480agaacacctt ttacaaatat aaatattcgc acactgttac aaacattatc atcaaattat 6540tgtggaattt tgcagacaaa

gcatatgcca ttgtgtttcc ttcgatttgg cttttatgct 6600tattcgtggt gtcataatta attcgtgaaa acgaatgatt ttacatactg taagtagggc 6660tgaaagcgga tcgaatagta ccatattttc tactatttgt gatttattcc atatttcagt 6720tttcttcgta aaaatacgaa tatccagttt ttcgaatatc cggaattttt ttgacatttg 6780cgaatattca cagatattta cagatgattc atccactttg ttcaatacaa atctaaaaaa 6840atatcaaatt attttttaga aatatctttt acatgataaa aatattaagt aaaaattaat 6900gaaactaaat gttttattaa attttaaaat tagtaatttt tcttataaaa atgtttttag 6960aaaaatatgc agttttgtat aaagaattat atatttttaa tttaatattt tataagtagt 7020ttttaaaata aaatttaata aattatatgt taaataatta tataagatta tacatttaaa 7080actctatatt taatcgtaga tatacatcta tttgtacaac tccgatccgt ctctaatttt 7140ttttagtatt tgtggtttgg ttcgttttta acggatattg atttttaata tttgcttttt 7200tttagagact tacgaatatt catatttttt tggatcgagt cgaaacaaat aataaattga 7260atcaatttaa cggataaaat gttcagccct aactatggtt aactatggat actgaaacca 7320ccacttatca acaaaaaaaa gtagttgttc gcttctggtt tagagtgagg ttacaaattc 7380aaaacataat aattattttc tcggacgata accagatcct ttttagaggc tttaaaatgt 7440aaatcatagt catgtgtagc ctatacatac aaattaaatt acaatggaat tttgaccaaa 7500aaaaaaaaaa aa 7512764034DNABrassica napus 76aagccagaaa aaaatgtgga agtgtaataa aacaattttc tccaggaaga agatatttca 60gccattcgcc tatttatgtg tatgagatcc cattcaagga catactgttt tccgtgagct 120actgaatgaa tacgatgctt ttgttgtaaa cgcaacctat tggttttttt tttccgccaa 180gttttgtttt tattaatcaa acgggccaag cccaaacggt cgaagcccaa atacactaag 240taccaaacaa aaggcccaca gacacaacgg gctacaacat taatggctga agcccaagta 300aactaaacgg cccaaaggcc caaacattga aaccggacag cttgacctag gcgcgcgata 360aggagactgg ggtttgaaca cgtgtaagaa tctgggccat tagtatgaca cgcgtcgccc 420gcctcgactt gatcgaaacc tcttcgcaca gccgcatgaa cttgccaccg acgacctcgt 480ttcgccggag ctcagagact gaagagcctc caacgaaacc atagctattc tacgccgctc 540tgtcttcacg ccggagagtt ccatcgatct aacgagatct cctccgacta tgtatcacca 600caaaccctag acaggcgagc cgagaaccac tgatatcctc ctcttgtacg tcctcgccgt 660tgtcggagag tttcatcatc atccgagatc tcatccgcga tggcccacga ccccatacct 720gaccacaaga accacaccgc acttatctca cctcgatcta atcaccacaa cccaaaggac 780atcgggttcc aagacggcag cgcggagctt tgcaagcctc cactctccgt gaccaaaagc 840cggcgaagac ggagctgaat gagcctcccc ttcccggaag ctaaagcggc gacggtggat 900ctgagaaagc ctccacctcc cgcataaaga ccggcgacga tggatctagg gtagcctcca 960tctcccggaa aaaacaccac ctgaacatgc aagccgctcc atctccaccg ggagcctcca 1020attgatcgcg tcgttactcc aaaggccgag ccaccgttga tggatggttg cggtcccaga 1080gctccgacaa ggcgatcgat gacgggactg cgaagaagga gcggagagtg acaactacaa 1140aggaagggaa agagctctgg cgacagcacg gacgctcacg cgccagccgt acgccgaacc 1200caaaagtaga tctggtttct ctcttttctc tctctcgcaa aagaaaacac gcaacctatt 1260gttctccatt ttgaatttga agtgttacat tattaataaa aaggaagaaa gtacaaagct 1320tttcatttag tggttgttgc atatatagta tacttatttg atgggcttat gataagtatg 1380aacatttcac gtaacgggtt tattttgtcg tcaacacatt accggcaatg tgaaaagaag 1440gggcgaggta tctccatttc aattcttctc tttatagatt aatcgaatta tttacatatg 1500aaatgagcgt ttatatagaa ttttcgtgtg gaaaacgaca tgtacacggc atcgaaagac 1560caattagtaa tatactttag tggtgattac atgtttactt atccaattga gaatttaaag 1620catcgacaat accttaatgt cgattaagcc gtccccactt catgtaatga gttatggggg 1680gagagagagg tcccgaaatt cgtcaaataa aacaacttag aactaaaaac cgacaccaag 1740tatcataaag gaaacgttga agaagtcatt tatcgtatcc agctcacaat tcctaagatt 1800aaatcatgac cgttggaaga gcttataaga ttaaattgaa gaaattgtgg gttttagaag 1860aaagacaaga aagagaagaa catgatctta cattgcctat tttggtgtat aggagttgtc 1920aaaaagagga gagagaggag acaattaggt caaataaatg agcactaaaa atggagacat 1980gtgttgagta actattacaa gagcgactta tgcttctaga accaagcctt tctctaatcc 2040ctaagatcat agctactgaa ataatgaaaa aaaacaaaaa aaaaacaatg aagaatcagt 2100tgggcattag tccaaaaaaa aaaaagaatc agttggattg cttataaaat tttgttataa 2160aatttatgtc gtatgtgtgt tagccgtagc tgtaaattta tttcctttgt atgttaattg 2220taaaatttaa tttattattc ccgatgtttt taatgatgta agtttacatc aagaaaacat 2280attaagttct gttaataaat gtattatttt tgtttactaa tttccaataa ttttttcaag 2340aagtacaatt ttttgtttga aatttctaat ttatcaacat acctaaaaac cataaacatg 2400tattttttga aacatttttt taatataaaa attatttttt aggagcaggt gaagtatttc 2460ttttaataaa attgttagtg tatttacgtt ggttatagtt gtagatattt tgcataataa 2520ttaataatat ataagatgtg taatgtatat atgtgagaga aataccatat ttactaagag 2580tttttcgcaa tattgactca aaattcaaag tcaatcacaa aactaacata tgtttctttt 2640tagacatttc ctttgcctta ttcactccac gggttcagat tattcacgaa aatgtcatta 2700aatttttttt cttttcgaaa atggcatttt tactctctca atttcatcct cttcaagtat 2760ttacaagatt tccattgcca tcaatacacc aaccacaatg aacaaccaat ttgaagctct 2820taatgcacct caaatcgatt tacacttctt ctttttcaat tcttatgaac taaaaacaac 2880atctctttca atttctcccc atattcatcc aaaaaaccca agatttttat tctaaaattt 2940taatggttca tagagccatt gaagcttacg attcttggtg ggtcactttt gttcgagatt 3000ttaggtactt ggagaagact tttgtatgct aaacaagtta tctcattggc tgaaactatg 3060aaatcagttt tttttccaga tctgttcgcc cggacgactt ccggataagt cttctggctg 3120tagacgactt acatcgaagt attctggtta atgcagatgt tagttttgca attgactttt 3180aagatgctta ctcgaagatt agtactcttc tgttcttgta ccttatgagc attccaatag 3240tattatattt ttcattctaa aatatatttc aatgatattt tacttttcac tctataatag 3300agtgaaaatg agtttacttt ataatcccct atatattatt tgtgaaacac tacaacttct 3360ttttgtagcc acatgtcatc actaggatga tttttagaat tattagagaa ataggttggt 3420ccatctaatt atataataag ttttttatta aattaaccat aaattcatta ttaatgtcat 3480ttattatttc cttaaataaa gattacggaa ttgcctaatg tgggtaaagt atatatgaca 3540attaatgatc ttgaataata aagatatgat aaaaaaaatg tatcttctat caaatttgtt 3600taatttaaaa ctattaaaat aatttttaaa aaaacacaat aaccatatta taaaaattta 3660gatttttctg tatattttta tattttgaac tttaaaaaat gacgataaat tactaaaact 3720gttaaaagtc tcacattcaa attttgctat ccatgtttta aaatttttgt tatgacaaaa 3780tacaaatgat tacaaaatca tataagtaaa agtctaattt aattaatcat taagatttaa 3840aatatatatg tatatatatc attctaaatt aaactataaa ccatattgaa taaataaata 3900ttttagtttc aaaattttac tttgaataat tttttttgat aaaagttttg aactaacatt 3960gataaatgtt tttaaactat caattaccaa aattattgat cccacaatga aaattttgtt 4020atcagtaatt taaa 4034774453DNABrassica napus 77ttaaacgtaa acgtctccgc tgtatttata taaacttttc aaaatcacgg tgtcgctcgt 60gttaagattt ggatttctaa tccaactttc agaaagttta tgtgaaagat aatactcaaa 120cacaatagtt cgtttagaac gctcttaaga ttaatgtcac acacacaaaa ttgatgtcac 180tagatttata tgttttgtcg tttaagggga tccatcaact acacatggaa gaatagaaaa 240cactggtcac acacaaaaaa taatataagc atgttgagtc ctattcatca gaaaattttc 300tccaaaggat gaacaataac ctacaattaa ggaaacttat tataacaacg accaagaagt 360tttcccataa gtcatgcttt cgtccatacc ttcgggtctt tcaagcaagc ttcacacatg 420ccttatcgta tatttgtata cgttttcatt ttgtgtacgt ctattaatta gtatatgcct 480agattatata tttatatagt cgacaattac ctatcctaaa ggtttatttt aatttttatt 540cagaaagata tgcacgtagg gccactagtg tttcccgttg ttattggaaa ttccatgtgt 600tgaactgttt gattaagttt ttgaacccgt aagtcaaatt tatttaggtt taacagggtt 660tcattactgt tataagcttg tcaagaaaga atttgtcaca aatttcgaga ctcatgcatg 720catgcatgtt tcttaacttt cgagaaacat gaagtgtggt tttaccagct gaagtttgtg 780ttaaaacaca tttttaattt gaccacgtgt gatattcaaa tatcaacaat tctgttcaac 840gggagattca tacatatgtt cagatgttct ttaatcttga gcacctaact ttgcttccca 900tacgcaaatg tgtgtgccgc gaactatggt gaatgtacgg catcgttttt ctttttggag 960catttttaaa agaaaatata atttagttat ttccgttttg attgatatag atgcattaat 1020aaaccgactt acgtgggtga tgggatgagc aaaattaaaa tatgcatttt agtaaccaat 1080tacggtccat cttgacaaac taacaaacaa aaagtctatt atgacgttga tttgtgacct 1140tgccatataa atgccaaaat ttcattcttt caataagcaa cgagatcaat aagatgtggc 1200agtcacgtga acgttagtta tcgccatttc aactgaacct tcacgaacat ataaagttca 1260tttgttcttt gctaaaatta cgcaaatctc tgttgtgcac gaaattacga ctatttttta 1320atgacaactc ctgttcttgt atcgtgtatc tagcacgtaa atttgaatta tggcggcatt 1380atcgataaaa tatgtaaaga atatattttc atcatacacc acaaacaaat gttctggaaa 1440tatcaccttt tatcacatat acaaagctga ccctgaattt ttggaaacta caagccaaaa 1500aaaaatggac aaaagaaaaa ttatgcacaa aagtggactc gtcctaactc tttttataat 1560gataattaac ctttgaacaa ctaattaata catacaatat gttttttttt tctaaaaatt 1620ggtcgattaa atattcatat ataatcctca tcatcattta ttagcctttt gtagcttact 1680taaaaaatca cttcccacca aattgattat tttttcactt catacgttga tcaatttaaa 1740aaaaaaataa taataatata tgcacgtccg gcgtcccatg acttcagacg cttttctgtt 1800tccttgattt tataggttag aaatttccaa aatgctcata tttttcactg atatgtatta 1860tacgcacaaa atagtaaagt cataaaaatt tatgatggtt cttttatatg tatagagtag 1920tgaaaattta tatatattgt agttccgctt aaaataaatt aatatttttc ttttatcgcc 1980catatttaag ttactaaacg tatataatta aaaactgaaa tgcaatatgt agaaatccgg 2040gcattgattg ttgattaaca ttgatatacc catgcgactg gcttatgatg aaccatacct 2100gctaatactc attgttacaa actcgcagca acatcataaa tccatcactc gccacaataa 2160ttttcttcag tgtatgttca attgatcaca ttttgaccct tcactttctt gaatgagaaa 2220ttatacaaga aagtgatttg agtgtgttaa aaaatgattt gtcattctga ccaattaggg 2280tttccactcc ctcacgctaa cacacatcac cgcagccatt tccatctctc aaccaacttc 2340aaacaatggc tgaacgaggc ggagaacgag gtgccgagcg aggaggagac cgcggcggtt 2400tcggacgcgg attcggagga ggtcgcggcg acagaggagg ccggggaggt ccgagaggcc 2460gaggcggcag acgcggagga cgtccgacag aggaggagaa gtggacccca gtgaccaagc 2520tcggccgtct cgtgcaagcc ggaaagatcc agaagctcga gcagatctac ctccactccc 2580tccccgtcaa ggagtaccag atcatcgacc tcctcgtcgg ccccaccttg aaagacgagg 2640tgatgaagat catgccggtt cagaaacaga ccagggccgg tcagaggacg aggttcaagg 2700cctttgtcgt cgtcggagac acgaacgggc acgttggttt gggtgtgaag tgctccaagg 2760aggtggcgac ggcgatcaga ggtgggatta tattggcgaa gctgtcggtt attcccgtga 2820ggagagggta ctgggggaac aagattggga agccgcatac ggttccttgt aaggttacgg 2880ggaagtgtgg gtctgttacg gtgaggatgg tgcctgctcc gaggggtgcg gggattgtgg 2940cggctagggt tcctaagaag gtgcttcagt tcgctgggat tgatgatgtg tttacttcgt 3000cgagggggtc tactaagact cttggaaact tcgtaaaggt ttgaactttc ttcatttatt 3060tagagtttgg agttgtgtat ggattgaaag ttgattcctt tttgatggtt gcgtgtggag 3120ttgttttggg taatttagct tgttgtagtt gatgtaaagt gagaattgct aagcttgatt 3180agtttgaata agctgtctgt gttaaatgat gatttgtttg tgttgtattg agctgtctga 3240atttcattat ttgtttgtgt tattgctaaa ttacaagctt aaatttgata acttttgatt 3300ctttcgtgag agtttttttt gggtaatata gcttgatgta gttgatggaa gtaaattgtt 3360aacctttaga gctgtctgtt aatggtgatt tgtttgtgtt gtattgagct gtctgaatta 3420taatttgttt gtgtttattg ctaaatcaca aagctttgaa gtcgtttgta catattcatc 3480atttttgtgt ttattgactt gtttgtgttt attgctaaat cacaagtttc tatgattaat 3540cttcatgatt tggttgtgtt tattactaaa tcacaagttt ctatgattaa tcttaatgat 3600ttggttgtgt ttattgctaa atttgagatt acattataat cttagtttat acatgtataa 3660catcatttca cttgaatttt cacagacgag ctggtgatat gtttttttag tattttccat 3720gtattgcttt gtaagaactt ttaaatgttt gttttcttcc tttgaagaac aattaaatgt 3780ttgttcagta tttttgaatc attcattggt taatcatgtg taattttatt tttgtaggct 3840acattcgact gtctccagaa gacttatgga ttcctgacac cagagttctg gaaagagacg 3900agctttaaaa agtcaccgta ccaggagtac actgatctgc tggccgagaa gggaattgca 3960accaaagcca tcaccgaggt tgaagaccaa gccgcctcct aaataaacaa acaaacaatg 4020tcccccttgt tttgtttttg gatgatcctt tcttgagttt tattttgttc gaacctttag 4080tatatgttgt ttgctggatt gctgctttct tatgttttca tcatctcccc caaacaaagt 4140tatttttgct ctagtttttt tttataatcg atggttatgc cctctagtat tttttttact 4200tgaatttttc actaattttc tatcaacttc cctatgttac actttctaag gttgattaca 4260taggtggatt gttgtgaaac aaatactaat aataaactaa tacttttaga ataatataat 4320tagtgggatt aaattataaa ttataaatta aaaagacaag tctgaaatgg attgtttaac 4380tgtaaaaggt aaagagactt tgaatagaag agaataaatc aaaagttgac gtatcttctc 4440cgaaatttca gct 445378188DNABrassica napus 78cattttgacc cttcactttc ttgaatgaaa aattatacaa gaaagtgatt tgagtgtgtt 60agaaaaaaaa aagatttgag tcattctgac caattagcta gggttttcgc tagctcacgc 120taacacacaa agccgcagcc atttccatct ctctcgcaac tcaacttcga acaatggctg 180aacgaggc 188794666DNABrassica napus 79gtcttcgcat atgatagatc ataagatttg gagaaacata taaaattaac ctatgaaatt 60aaggggatct atatattata gtatgtatcc aaagaacata tatatgaaat atttgaatac 120atcataagat ttggggaata taaattaata taaaataaaa tcgtccaatt ttaaacgtaa 180acgtctccgc tgtatttata taaacttttc aaaatcacgg tgtcgctcgt gttaagattt 240ggatttctaa tccaactttc agaaagttta tgtgaaagat aatactcaaa cacaatagtt 300cgtttagaac gctcttaaga ttaatgtcac acacacaaaa ttgatgtcac tagatttata 360tgttttgtcg tttaagggga tccatcaact acacatggaa gaatagaaaa cactggtcac 420acacaaaaaa taatataagc atgttgagtc ctattcatca gaaaattttc tccaaaggat 480gaacaataac ctacaattaa ggaaacttat tataacaacg accaagaagt tttcccataa 540gtcatgcttt cgtccatacc ttcgggtctt tcaagcaagc ttcacacatg ccttatcgta 600tatttgtata cgttttcatt ttgtgtacgt ctattaatta gtatatgcct agattatata 660tttatatagt cgacaattac ctatcctaaa ggtttatttt aatttttatt cagaaagata 720tgcacgtagg gccactagtg tttcccgttg ttattggaaa ttccatgtgt tgaactgttt 780gattaagttt ttgaacccgt aagtcaaatt tatttaggtt taacagggtt tcattactgt 840tataagcttg tcaagaaaga atttgtcaca aatttcgaga ctcatgcatg catgcatgtt 900tcttaacttt cgagaaacat gaagtgtggt tttaccagct gaagtttgtg ttaaaacaca 960tttttaattt gaccacgtgt gatattcaaa tatcaacaat tctgttcaac gggagattca 1020tacatatgtt cagatgttct ttaatcttga gcacctaact ttgcttccca tacgcaaatg 1080tgtgtgccgc gaactatggt gaatgtacgg catcgttttt ctttttggag catttttaaa 1140agaaaatata atttagttat ttccgttttg attgatatag atgcattaat aaaccgactt 1200acgtgggtga tgggatgagc aaaattaaaa tatgcatttt agtaaccaat tacggtccat 1260cttgacaaac taacaaacaa aaagtctatt atgacgttga tttgtgacct tgccatataa 1320atgccaaaat ttcattcttt caataagcaa cgagatcaat aagatgtggc agtcacgtga 1380acgttagtta tcgccatttc aactgaacct tcacgaacat ataaagttca tttgttcttt 1440gctaaaatta cgcaaatctc tgttgtgcac gaaattacga ctatttttta atgacaactc 1500ctgttcttgt atcgtgtatc tagcacgtaa atttgaatta tggcggcatt atcgataaaa 1560tatgtaaaga atatattttc atcatacacc acaaacaaat gttctggaaa tatcaccttt 1620tatcacatat acaaagctga ccctgaattt ttggaaacta caagccaaaa aaaaatggac 1680aaaagaaaaa ttatgcacaa aagtggactc gtcctaactc tttttataat gataattaac 1740ctttgaacaa ctaattaata catacaatat gttttttttt tctaaaaatt ggtcgattaa 1800atattcatat ataatcctca tcatcattta ttagcctttt gtagcttact taaaaaatca 1860cttcccacca aattgattat tttttcactt catacgttga tcaatttaaa aaaaaaataa 1920taataatata tgcacgtccg gcgtcccatg acttcagacg cttttctgtt tccttgattt 1980tataggttag aaatttccaa aatgctcata tttttcactg atatgtatta tacgcacaaa 2040atagtaaagt cataaaaatt tatgatggtt cttttatatg tatagagtag tgaaaattta 2100tatatattgt agttccgctt aaaataaatt aatatttttc ttttatcgcc catatttaag 2160ttactaaacg tatataatta aaaactgaaa tgcaatatgt agaaatccgg gcattgattg 2220ttgattaaca ttgatatacc catgcgactg gcttatgatg aaccatacct gctaatactc 2280attgttacaa actcgcagca acatcataaa tccatcactc gccacaataa ttttcttcag 2340tgtatgttca attgatcaca ttttgaccct tcactttctt gaatgagaaa ttatacaaga 2400aagtgatttg agtgtgttaa aaaatgattt gtcattctga ccaattagct agggtttcca 2460ctccctcacg ctaacacaca tcaccgcagc catttccatc tctcaaccaa cttcaaacaa 2520tggctgaacg aggcggagaa cgaggtgccg agcgaggagg agaccgcggc ggtttcggac 2580gcggattcgg aggaggtcgc ggcgacagag gaggccgggg aggtccgaga ggccgaggcg 2640gcagacgcgg aggacgtccg acagaggagg agaagtggac cccagtgacc aagctcggcc 2700gtctcgtgca agccggaaag atccagaagc tcgagcagat ctacctccac tccctccccg 2760tcaaggagta ccagatcatc gacctcctcg tcggccccac cttgaaagac gaggtgatga 2820agatcatgcc ggttcagaaa cagaccaggg ccggtcagag gacgaggttc aaggcctttg 2880tcgtcgtcgg agacacgaac gggcacgttg gtttgggtgt gaagtgctcc aaggaggtgg 2940cgacggcgat cagaggtggg attatattgg cgaagctgtc ggttattccc gtgaggagag 3000ggtactgggg gaacaagatt gggaagccgc atacggttcc ttgtaaggtt acggggaagt 3060gtgggtctgt tacggtgagg atggtgcctg ctccgagggg tgcggggatt gtggcggcta 3120gggttcctaa gaaggtgctt cagttcgctg ggattgatga tgtgtttact tcgtcgaggg 3180ggtctactaa gactcttgga aacttcgtaa aggtttgaac tttcttcatt tatttagagt 3240ttggagttgt gtatggattg aaagttgatt cctttttgat ggttgcgtgt ggagttgttt 3300tgggtaattt agcttgttgt agttgatgta aagtgagaat tgctaagctt gattagtttg 3360aataagctgt ctgtgttaaa tgatgatttg tttgtgttgt attgagctgt ctgaatttca 3420ttatttgttt gtgttattgc taaattacaa gcttaaattt gataactttt gattctttcg 3480tgagagtttt ttttgggtaa tatagcttga tgtagttgat ggaagtaaat tgttaacctt 3540tagagctgtc tgttaatggt gatttgtttg tgttgtattg agctgtctga attataattt 3600gtttgtgttt attgctaaat cacaaagctt tgaagtcgtt tgtacatatt catcattttt 3660gtgtttattg acttgtttgt gtttattgct aaatcacaag tttctatgat taatcttcat 3720gatttggttg tgtttattac taaatcacaa gtttctatga ttaatcttaa tgatttggtt 3780gtgtttattg ctaaatttga gattacatta taatcttagt ttatacatgt ataacatcat 3840ttcacttgaa ttttcacaga cgagctggtg atatgttttt ttagtatttt ccatgtattg 3900ctttgtaaga acttttaaat gtttgttttc ttcctttgaa gaacaattaa atgtttgttc 3960agtatttttg aatcattcat tggttaatca tgtgtaattt tatttttgta ggctacattc 4020gactgtctcc agaagactta tggattcctg acaccagagt tctggaaaga gacgagcttt 4080aaaaagtcac cgtaccagga gtacactgat ctgctggccg agaagggaat tgcaaccaaa 4140gccatcaccg aggttgaaga ccaagccgcc tcctaaataa acaaacaaac aatgtccccc 4200ttgttttgtt tttggatgat cctttcttga gttttatttt gttcgaacct ttagtatatg 4260ttgtttgctg gattgctgct ttcttatgtt ttcatcatct cccccaaaca aagttatttt 4320tgctctagtt tttttttata atcgatggtt atgccctcta gtattttttt tacttgaatt 4380tttcactaat tttctatcaa cttccctatg ttacactttc taaggttgat tacataggtg 4440gattgttgtg aaacaaatac taataataaa ctaatacttt tagaataata taattagtgg 4500gattaaatta taaattataa attaaaaaga caagtctgaa atggattgtt taactgtaaa 4560aggtaaagag actttgaata gaagagaata aatcaaaagt tgacgtatct tctccgaaat 4620ttcagctatt tctaaatacg cggattctga ttactctgtt ctctct 466680185DNABrassica napus 80cattttgacc cttcactttc ttgaatgaaa attatacaag aaaatgattt gagtgtgtta 60aaaaaaaaat gttttgagtc attctgacca atagctaggg ttttccctag ctcacgctaa 120cacacaaagc cgcagccatt tccatctctc tcgcaactca acttcgaaca atggctgaac 180gaggc 1858118DNAArtificial Sequencesynthesized oligonucleotide 81tctctttcct tcttcatc 188219DNAArtificial Sequencesynthesized oligonucleotide 82cttggttcta gaagcataa

198327DNAArtificial Sequencesynthesized oligonucleotide 83aaaatggaga catgtgttga gtaacta 278421DNAArtificial Sequencesynthesized oligonucleotide 84tttggactaa tgcccaactg a 218522DNAArtificial Sequencesynthesized oligonucleotide 85tgatatcatc accaaagtgc aa 228624DNAArtificial Sequencesynthesized oligonucleotide 86caaaatcatg gttactcaaa aagc 248718DNAArtificial Sequencesynthesized oligonucleotide 87tctctttcct tcttcatc 188819DNAArtificial Sequencesynthesized oligonucleotide 88cttggttcta gaagcataa 198927DNAArtificial Sequencesynthesized oligonucleotide 89aaaatggaga catgtgttga gtaacta 279021DNAArtificial Sequencesynthesized oligonucleotide 90tggactaatg cccaactgat t 219122DNAArtificial Sequencesynthesized oligonucleotide 91tgatatcatc accaaagtgc aa 229224DNAArtificial Sequencesynthesized oligonucleotide 92caaaatcatg gttactcaaa aagc 249317DNAArtificial Sequencesynthesized oligonucleotide 93cttattccag acatttg 179415DNAArtificial Sequencesynthesized oligonucleotide 94cttggttcag aagca 159527DNAArtificial Sequencesynthesized oligonucleotide 95aaaatggaga catgtgttga gtaacta 279626DNAArtificial Sequencesynthesized oligonucleotide 96cttcattgtt tcattgtttc agtagc 269728DNAArtificial Sequencesynthesized oligonucleotide 97tggatataac atatgaatcg gataagtg 289822DNAArtificial Sequencesynthesized oligonucleotide 98ttagagaaag gcttggttct cc 229917DNAArtificial Sequencesynthesized oligonucleotide 99cttattccag acatttg 1710015DNAArtificial Sequencesynthesized oligonucleotide 100cttggttcag aagca 1510127DNAArtificial Sequencesynthesized oligonucleotide 101aaaatggaga catgtgttga gtaacta 2710226DNAArtificial Sequencesynthesized oligonucleotide 102cttcattgtt tcattgtttc agtagc 2610328DNAArtificial Sequencesynthesized oligonucleotide 103ggatataaca tatgaatcgg ataagtgt 2810422DNAArtificial Sequencesynthesized oligonucleotide 104ttagagaaag gcttggttct cc 22105549DNABrassica napus 105atgtctggct caaaagcaga tgcagccata gccccaatag tcatgatgga gcatcatcat 60ctccttatga attggaacaa acctattgat ctcattacag aagaaaactc ttttaaccac 120aatcctcatt tcatagtaga tccaccttcc gaaaccctaa gccacttcca gcccccgccg 180acaatcttct ccgatcacgg aggaggagag gaagcagaag aagaagaagg agaggaagag 240atggatccga tgaagaagat gcaatacgcg attgctgcca tgcagcccgt agacctcgat 300ccagccaccg ttcctaagcc gaaccgccgt aacgtaaggg taagcgacga ccctcagacg 360gtggtggctc gtcggcgtag agaaaggata agcgagaaga tccggatatt gaagaggatg 420gtgccaggcg gtgcaaagat ggacactgcc tccatgctcg acgaagccat ccgctacacc 480aagttcttga aacggcaggt gaggctagct tcttcagcct cacactcagc ttggagctcc 540tatgtctga 549106558DNABrassica napus 106atgtctggct caaaagcaga tgcagccata gccccaatag tcatgatgga gcatcatcat 60ctccttatga attggaacaa acctattgat ctcattacag aagaaaactc ttttaaccac 120aatcctcatt tcatagtaga tccaccttcc gaaaccctaa gccacttcca gcccccgccg 180acaatcttct ccgatcacgg aggaggagag gaagcagaag aagaagaaga agaagaagga 240gaggaagaga tggatccgat gaagaagatg caatacgcga ttgctgccat gcagcccgta 300gacctcgatc cagccaccgt tcctaagccg aaccgccgta acgtaagggt aagcgacgac 360cctcagacgg tggtggctcg tcggcgtaga gaaaggataa gcgagaagat ccggatattg 420aagaggatgg tgccaggcgg tgcaaagatg gacactgcct ccatgctcga cgaagccatc 480cgctacacca agttcttgaa acggcaggtg aggctagctt cttcagcctc acactcagct 540tggagctcct atgtctga 558107549DNABrassica napus 107atgtctggct caaaagcaga tgcagccata gccccaatag tcatgatgga gcatcatcat 60ctccttatga attggaacaa acctattgat ctcattacag aagaaaactc ttttaaccac 120aatcctcatt tcatagtaga tccaccttcc gaaaccctaa gccacttcca gcccccgccg 180acaatcttct ccgatcacgg aggaggagag gaagcagaag aagaagaagg agaggaagag 240atggatccga tgaagaagat gcaatacgcg attgctgcca tgcagcccgt agacctcgat 300ccagccaccg ttcctaagcc gaaccgccgt aacgtaaggg taagcgacga ccctcagacg 360gtggtggctc gtcggcgtag agaaaggata agcgagaaga tccggatatt gaagaggatg 420gtgccaggcg gtgcaaagat ggacactgcc tccatgctcg acgaagccat ccgctacacc 480aagttcttga aacggcaggt gaggctagct tcttcagcct cacactcagc ttggagctcc 540tatgtctga 549108537DNABrassica napus 108atgtctggtt caaaagcaga tgcagcagcc atagccccaa tagtcatgat ggagcctcat 60catctcctta tgaactggaa caaacctatt gatctcatta cacaagaaaa ctcttttaac 120cacaatcctc atttcatggt agatccacct tccgaaaccc taagccactt ccagcccccg 180ccgacagaag agatagatga gatgaaggag atgcaatacg cgattgctgc catgcagccc 240gtagacatcg atccagccac cgttcctaag ccgaaccgcc gtaacgtaag ggtaagcgag 300gacccccaga cggtggtggc tcgtcggcgt agagaaagga taagcgagaa gatccggata 360ttgaagagga tggtgccagg cggtgcaaag atggacactg cctccatgct tgacgaagcc 420atccgctaca ccaagttctt gaaacggcag gtgaggcttc ttcagcctca cactcagctt 480ggggctccta tgtctgaccc ttctcgcctt tgttattacc acaactcgga tacctaa 537109537DNABrassica napus 109atgtctggtt caaaagcaga tgcagcagcc atagccccaa tagtcatgat ggagcctcat 60catctcctta tgaactggaa caaacctatt gatctcatta cacaagaaaa ctcttttaac 120cacaatcctc atttcatggt agatccacct tccgaaaccc taagccactt ccagcccccg 180ccgacagaag agatagatga gatgaaggag atgcaatacg cgattgctgc catgcagccc 240gtagacatcg atccagccac cgttcctaag ccgaaccgcc gtaacgtaag ggtaagcgag 300gacccccaga cggtggtggc tcgtcggcgt agagaaagga taagcgagaa gatccggata 360ttgaagagga tggtgccagg cggtgcaaag atggacactg cctccatgct tgacgaagcc 420atccgctaca ccaagttctt gaaacggcag gtgaggcttc ttcagcctca cactcagctt 480ggggctccta tgtctgaccc ttctcgcctt tgttattacc acaactcgga tacctaa 537110537DNABrassica napus 110atgtctggtt caaaagcaga tgcagcagcc atagccccaa tagtcatgat ggagcctcat 60catctcctta tgaactggaa caaacctatt gatctcatta cacaagaaaa ctcttttaac 120cacaatcctc atttcatggt agatccacct tccgaaaccc taagccactt ccagcccccg 180ccgacagaag agatagatga gatgaaggag atgcaatacg cgattgctgc catgcagccc 240gtagacatcg atccagccac cgttcctaag ccgaaccgcc gtaacgtaag ggtaagcgag 300gacccccaga cggtggtggc tcgtcggcgt agagaaagga taagcgagaa gatccggata 360ttgaagagga tggtgccagg cggtgcaaag atggacactg cctccatgct tgacgaagcc 420atccgctaca ccaagttctt gaaacggcag gtgaggcttc ttcagcctca cactcagctt 480ggggctccta tgtctgaccc ttctcgcctt tgttattacc acaactcgga tacctaa 537111537DNABrassica napus 111atgtctggtt caaaagcaga tgcagcagcc atagccccaa tagtcatgat ggagcctcat 60catctcctta tgaactggaa caaacctatt gatctcatta cacaagaaaa ctcttttaac 120cacaatcctc atttcatggt agatccacct tccgaaaccc taagccactt ccagcccccg 180ccgacagaag agatagatga gatgaaggag atgcaatacg cgattgctgc catgcagccc 240gtagacatcg atccagccac cgttcctaag ccgaaccgcc gtaacgtaag ggtaagcgag 300gacccccaga cggtggtggc tcgtcggcgt agagaaagga taagcgagaa gatccggata 360ttgaagagga tggtgccagg cggtgcaaag atggacactg cctccatgct tgacgaagcc 420atccgctaca ccaagttctt gaaacggcag gtgaggcttc ttcagcctca cactcagctt 480ggggctccta tgtctgaccc ttctcgcctt tgttattacc acaactcgga tacctaa 5371121302DNABrassica napus 112atggctcatt gttgcacagt tcttaccggt ttactagtat ggtctctttt tatgctctca 60tggtgtgaag cttcaagaaa caacattaat agatatgatc attcttatgg aacgttcaaa 120tccaatagct taatcaagcg aagagacgat ataactcgtc tgaaaagtgt tgcaagagcc 180tctttgcggc ttccaaccac cgttagtgtt tctgattttg gagctaaagg agatggaaaa 240accgacgaca cgcaggcgtt cgtgaatgca tggaagaaag cttgttcttc aagtggagct 300gtaaatctct tagttcctga aggaaaaact tattttctca agtctcttcg attaaatggc 360ccatgcaaat cagttcttac cgttcagatt ttaggaacgt tatcggcttc tcaacaacga 420tcggattatg aagatctcag caaatggatt acgtttgacg gcgttaacag tctaacggtt 480gatggcggag ccaccggaac tgtcaacgga aacggcgaaa cgtggtggga aaactcatgc 540aaacggaacg aagctaagaa atgcacaaaa gcccctacgg ctcttacttt ctacaactcg 600aagaatttaa gagtgaataa tctgagggtg aaagatgcgc aacagattca gatttcgatt 660gagaaatgct ccaacgttca ggtctcaaat gttgaggtca ctgcacctgc tgatagtcct 720aacactgatg gtatccacat cactaacact caaaacattc aagtctccaa ttccatcatt 780ggaacaggtg atgattgtat atctattgaa agtggatcac aaaacgttaa aatcaacgat 840ttaacttgcg gctcaggcca cggtatcagc attgggagct taggagatga caattcaaag 900gcatttgtat caggcgtgac tgtggatggt gctaaacttt ccggtacaga caatggagtt 960aggatcaaaa cttaccaggg agggtctgga actgctagca acattatatt tcagaacatt 1020cagatggaga atgtgaagaa tccaatcata attgaccaag attattgcga caagagcaaa 1080tgcactgaac aaaaatcagc ggtccaaata aagaacgtgg tgtaccggaa cataagcggc 1140acaagcgcat cagacatagc aataactttc aactgcagca agaactatcc atgccaagga 1200attgtactcg acaaagtgaa cattaaggga gggaaagcca gttgcagcaa tgctaatgtg 1260atggataaag acggtgtttt acctcagtgc aaatccactt ga 13021131302DNABrassica napus 113atggctcatt gttgcacagt tcttaccggt ttactagtat ggtctctttt tatgctctca 60tggtgtgaag cttcaagaaa caacattaat agatatgatc attcttatgg aacgttcaaa 120tccaatagct taatcaagcg aagagacgat atcactcgtc tgaaaagtgt tgcaagagct 180tctttgcggc ttccaaccac cgttagtgtt tctgattttg gagctaaagg agatggaaaa 240accgacgaca cgcaggcgtt cgtgaatgca tggaagaaag cttgttcttc aagtggagct 300gtaaatctct tagttcctga aggaaaaact tattttctca agtctcttcg attaaatggc 360ccatgcaaat cagttcttac cgttcagatt ttaggaacgt tatcggcttc tcaacaacga 420tcggattatg aagatctcag caaatggatt acgtttgacg gcgttaacag tctaacggtt 480gatggcggag ccaccggaac tgtcaacgga aacggcgaaa cgtggtggga aaactcatgc 540aaacggaacg aagctaagaa atgcacaaaa gcccctacgg ctcttacttt ctacaactcg 600aagaatttga gagttaataa tctgagggtg aaagatgcgc aacagattca gatttcgatt 660gagaaatgct ccaacgttca ggtctcaaat gttgaggtca ctgcacctgc tgatagtcct 720aacactgatg gtatccacat cactaacact caaaacattc aagtctccaa ttccatcatt 780ggaacaggtg atgattgtat atctattgaa agtggatcac aaaacgttaa aatcaacgat 840ttaacttgcg gcccaggcca cggtatcagc attgggagct taggagatga caattcaaag 900gcatttgtat caggcgtgac tgtggatggt gctaaacttt ccggtacaga caatggagtt 960aggatcaaaa cttaccaggg agggtctgga actgctagca acattatatt tcagaacatt 1020cagatggaga atgtgaagaa tccaatcata attgaccaag attattgcga caagagcaaa 1080tgcactgaac aaaaatcagc ggtccaaata aagaacgtgg tgtaccggaa cataagcggc 1140acaagcgcat cggacatagc aataactttc aactgcagca agaactatcc atgccaagga 1200attgtactcg acaaagtgaa cattaaggga gggaaagcca gttgcagcaa tgctaatgtg 1260atggataaag acggtgtttt acctcagtgc aaatccactt ga 13021141302DNABrassica napus 114atggctcatt gttgcacagt tcttaccggt ttactagtat ggtctctttt tatgctctca 60tggtgtgaag cttcaagaaa caacattaat agatatgatc attcttatgg aacgttcaaa 120tccaatagct taatcaagcg aagagacgat ataactcgtc tgaaaagtgt tgcaagagcc 180tctttgcggc ttccaaccac cgttagtgtt tctgattttg gagctaaagg agatggaaaa 240accgacgaca cgcaggcgtt cgtgaatgca tggaagaaag cttgttcttc aagtggagct 300gtaaatctct tagttcctga aggaaaaact tattttctca agtctcttcg attaaatggc 360ccatgcaaat cagttcttac cgttcagatt ttaggaacgt tatcggcttc tcaacaacga 420tcggattatg aagatctcag caaatggatt acgtttgacg gcgttaacag tctaacggtt 480gatggcggag ccaccggaac tgtcaacgga aacggcgaaa cgtggtggga aaactcatgc 540aaacggaacg aagctaagaa atgcacaaaa gcccctacgg ctcttacttt ctacaactcg 600aagaatttaa gagtgaataa tctgagggtg aaagatgcgc aacagattca gatttcgatt 660gagaaatgct ccaacgttca ggtctcaaat gttgaggtca ctgcacctgc tgatagtcct 720aacactgatg gtatccacat cactaacact caaaacattc aagtctccaa ttccatcatt 780ggaacaggtg atgattgtat atctattgaa agtggatcac aaaacgttaa aatcaacgat 840ttaacttgcg gcccaggcca cggtatcagc attgggagct taggagatga caattcaaag 900gcatttgtat caggcgtgac tgtggatggt gctaaacttt ccggtacaga caatggagtt 960aggatcaaaa cttaccaggg agggtctgga actgctagca acattatatt tcagaacatt 1020cagatggaga atgtgaagaa tccaatcata attgaccaag attattgcga caagagcaaa 1080tgcactgaac aaaaatcagc ggtccaaata aagaacgtgg tgtaccggaa cataagcggc 1140acaagcgcat cggacatagc aataactttc aactgcagca agaactatcc atgccaagga 1200attgtactcg acaaagtgaa cattaaggga gggaaagcca gttgcagcaa tgctaatgtg 1260atggataaag acggtgtttt acctcagtgc aaatccactt ga 13021151302DNABrassica napus 115atggctcatt gttgcacagt tcttaccggt ttactagtat ggtctctttt tatgctctca 60tggtgtgaag cttcaagaaa caacattaat agatatgatc attcttatgg aacgttcaaa 120tccaatagct taatcaagcg aagagacgat ataactcgtc tgaaaagtgt tgcaagagcc 180tctttgcggc ttccaaccac cgttagtgtt tctgattttg gagctaaagg agatggaaaa 240accgacgaca cgcaggcgtt cgtgaatgca tggaagaaag cttgttcttc aagtggagct 300gtaaatctct tagttcctga aggaaaaact tattttctca agtctcttcg attaaatggc 360ccatgcaaat cagttcttac cgttcagatt ttaggaacgt tatcggcttc tcaacaacga 420tcggattatg aagatctcag caaatggatt acgtttgacg gcgttaacag tctaacggtt 480gatggcggag ccaccggaac tgtcaacgga aacggcgaaa cgtggtggga aaactcatgc 540aaacggaacg aagctaagaa atgcacaaaa gcccctacgg ctcttacttt ctacaactcg 600aagaatttaa gagtgaataa tctgagggtg aaagatgcgc aacagattca gatttcgatt 660gagaaatgct ccaacgttca ggtctcaaat gttgaggtca ctgcacctgc tgatagtcct 720aacactgatg gtatccacat cactaacact caaaacattc aagtctccaa ttccatcatt 780ggaacaggtg atgattgtat atctattgaa agtggatcac aaaacgttaa aatcaacgat 840ttaacttgcg gcccaggcca cggtatcagc attgggagct taggagatga caattcaaag 900gcatttgtat caggcgtgac tgtggatggt gctaaacttt ccggtacaga caatggagtt 960aggatcaaaa cttaccaggg agggtctgga actgctagca acattatatt tcagaacatt 1020cagatggaga atgtgaagaa tccaatcata attgaccaag attattgcga caagagcaaa 1080tgcactgaac aaaaatcagc ggtccaaata aagaacgtgg tgtaccggaa cataagcggc 1140acaagcgcat cggacatagc aataactttc aactgcagca agaactatcc atgccaagga 1200attgtactcg acaaagtgaa cattaaggga gggaaagcca gttgcagcaa tgctaatgtg 1260atggataaag acggtgtttt acctcagtgc aaatccactt ga 13021162303DNABrassica napus 116atggctcatt gttggacagt tcttaccggt ttactagtat ggtctctttt gatgctctca 60tggtgcgaag cttcaagaaa caacattaat agatatgatc attcttatgg aacgttcaaa 120tccaatagct taatcaagcg aagagacgat atcactcgtc tgaaaagtgt tgcaagagct 180tctttgcggc ttccaaccac cgttagtgtt tctgattttg gagctaaagg agatggaaaa 240accgacgaca cgcaggcgtt cgtgaatgca tggaagaaag cttgttcttc aagtggacct 300gtaaatctct tagttcctga aggaaaaact tattttctca agtctcttcg attaaatggc 360ccatgcaaat cagttcttac cgttcagatt ttaggaacgt tatcggcatc tcaacaacga 420tcggattata aagatctcag caaatggatt acgtttgacg gcgttaacag tctaacggtt 480gatggcgggg ccaccggaac tgtcaacgga aacggcgaaa cgtggtggga aaactcatgc 540aaacggaacg aagctaagaa atgcacaaaa gccccaacgg ctcttacttt ctacaactcg 600aagaatttga gagttaataa tctgagggtg aaagatgcgc aacagatcca gatttcgatt 660gagaaatgct ccaacgttca ggtctcaaat gttgaggtca ctgcgcctgc tgatagtcct 720aacactgatg gtatccacat cactaacact caaaacattc aagtctccaa atccatcatt 780ggaacaggtg atgattgtat atctattgaa agtggatcac aaaacgttaa aatcaacgat 840ttaacttgcg gccccggtca cggtatcagc attgggagct taggagatga caattcaaag 900gcatttgtat caggcgtgac tgtggatggt gctaaacttt ccggtacaga caatggagtt 960aggatcaaaa cttaccaggg agggtctgga actgctagca aatggctcat tgttggacag 1020ttcttaccgg tttactagta tggtctcttt tgatgctctc atggtgcgaa gcttcaagaa 1080acaacattaa tagatatgat cattcttatg gaacgttcaa atccaatagc ttaatcaagc 1140gaagagacga tatcactcgt ctgaaaagtg ttgcaagagc ttctttgcgg cttccaacca 1200ccgttagtgt ttctgatttt ggagctaaag gagatggaaa aaccgacgac acgcaggcgt 1260tcgtgaatgc atggaagaaa gcttgttctt caagtggacc tgtaaatctc ttagttcctg 1320aaggaaaaac ttattttctc aagtctcttc gattaaatgg cccatgcaaa tcagttctta 1380ccgttcagat tttaggaacg ttatcggcat ctcaacaacg atcggattat aaagatctca 1440gcaaatggat tacgtttgac ggcgttaaca gtctaacggt tgatggcggg gccaccggaa 1500ctgtcaacgg aaacggcgaa acgtggtggg aaaactcatg caaacggaac gaagctaaga 1560aatgcacaaa agccccaacg gctcttactt tctacaactc gaagaatttg agagttaata 1620atctgagggt gaaagatgcg caacagatcc agatttcgat tgagaaatgc tccaacgttc 1680aggtctcaaa tgttgaggtc actgcgcctg ctgatagtcc taacactgat ggtatccaca 1740tcactaacac tcaaaacatt caagtctcca aatccatcat tggaacaggt gatgattgta 1800tatctattga aagtggatca caaaacgtta aaatcaacga tttaacttgc ggccccggtc 1860acggtatcag cattgggagc ttaggagatg acaattcaaa ggcatttgta tcaggcgtga 1920ctgtggatgg tgctaaactt tccggtacag acaatggagt taggatcaaa acttaccagg 1980gagggtctgg aactgctagc aacataatat ttcagaacat tcagatggag aatgtgaaga 2040atccaatcat aatcgaccaa gattattgcg acaagagcaa atgcaccgaa caaaaatcag 2100cggtccaaat caagaacgtg gtgtaccgga acataagcgg cacaagcgca tcagacatag 2160caataacttt caactgtagc aagaactatc catgccaagg aattgtactc gacaaagtga 2220acattaaggg agggaaagcg agttgcagca acgctaatgt gatggataaa gacggtgttt 2280tgcctcagtg caaatccact tga 23031171302DNABrassica napus 117atggctcatt gttggacagt tcttaccggt ttactagtat ggtctctttt gatgctctca 60tggtgcgaag cttcaagaaa caacattaat agatatgatc attcttatgg aacgttcaaa 120tccaatagct taatcaagcg aagagacgat atcactcgtc tgaaaagtgt tgcaagagct 180tctttgcggc ttccaaccac cgttagtgtt tctgattttg gagctaaagg agatggaaaa 240accgacgaca cgcaggcgtt cgtgaatgca tggaagaaag cttgttcttc aagtggacct 300gtaaatctct tagttcctga aggaaaaact tattttctca agtctcttcg attaaatggc 360ccatgcaaat cagttcttac cgttcagatt ttaggaacgt tatcggcatc tcaacaacga 420tcggattata aagatctcag caaatggatt

acgtttgacg gcgttaacag tctaacggtt 480gatggcgggg ccaccggaac tgtcaacgga aacggcgaaa cgtggtggga aaactcatgc 540aaacggaacg aagctaagaa atgcacaaaa gccccaacgg ctcttacttt ctacaactcg 600aagaatttga gagttaataa tctgagggtg aaagatgcgc aacagatcca gatttcgatt 660gagaaatgct ccaacgttca ggtctcaaat gttgaggtca ctgcgcctgc tgatagtcct 720aacactgatg gtatccacat cactaacact caaaacattc aagtctccaa atccatcatt 780ggaacaggtg atgattgtat atctattgaa agtggatcac aaaacgttaa aatcaacgat 840ttaacttgcg gccccggtca cggtatcagc attgggagct taggagatga caattcaaag 900gcatttgtat caggcgtgac tgtggatggt gctaaacttt ccggtacaga caatggagtt 960aggatcaaaa cttaccaggg agggtctgga actgctagca acataatatt tcagaacatt 1020cagatggaga atgtgaagaa tccaatcata atcgaccaag attattgcga caagagcaaa 1080tgcaccgaac aaaaatcagc ggtccaaatc aagaacgtgg tgtaccggaa cataagcggc 1140acaagcgcat cagacatagc aataactttc aactgtagca agaactatcc atgccaagga 1200attgtactcg acaaagtgaa cattaaggga gggaaagcga gttgcagcaa cgctaatgtg 1260atggataaag acggtgtttt gcctcagtgc aaatccactt ga 13021181302DNABrassica napus 118atggctcatt gttgcacagt tcttaccggt ttactagtat ggtctctttt gatgctctca 60tggtgcgaag cttcaagaaa caacattaat agatatgatc attcttatgg aacgttcaaa 120tccaatagct taatcaagcg aagagacgat atcactcgtc tgaaaagtgt tgcaagagcc 180tctttgcagc ttccaaccac cgttagtgtt tctgattttg gagctaaagg agatggaaaa 240accgacgaca cgcaggcgtt cgtgaatgca tggaagaaag cttgttcttc aagtggagct 300gtaaatctct tagttcctga aggaaaaact tatttcctta agtctattcg attaaatggc 360ccatgcaaat caattcttac cgttcagatt ttaggaacgc tatcggcatc tcaacaacga 420tcggattata aagatctcag caaatggatt acgtttgacg gcgttaacag tctaacggtt 480gatggcgggg ccaccggaac tgtcaacgga aacggcgaaa cgtggtggga aaactcatgc 540aaacggaacg aagctaagaa atgcacaaaa gccccaacgg ctcttacttt ctacaactca 600aagaatttga gagttaataa tctgagggtg aaagatgcgc aacagatcca gatttcgatt 660gagaaatgct ccaacgttca ggtctcaaat gttgaggtca ctgcgcctgc tgatagtcct 720aacactgatg gtatccacat cactaacact caaaacattc aagtctccaa atccatcatt 780ggaacaggtg atgattgtat atctattgaa agtggatcac aaaacgttaa aatcaacgat 840ttaacttgcg gccccggtca cggtatcagc attgggagct taggagatga caattcaaag 900gcatttgtat caggcgtgac tgtggatggt gctaaacttt ccggtacaga caatggagtt 960aggatcaaaa cttaccaggg agggtctgga actgctagca acattatatt tcagaacatt 1020cagatggaaa atgtgaagaa tccaattata atcgaccaag attattgcga caagagcaaa 1080tgcactgaac aaaaatcagc ggtccaaata aagaacgtgg tgtaccggaa cataagcggc 1140acaagcgcat cggacatagc aataactttc aactgcagca agaactatcc atgccaagga 1200attgtagtcg acaaagtgaa cattaaggga gggaaagcaa gttgcagcaa cgctaatgtg 1260atggataaag acggtgtttt gcctcagtgc aaatccactt ga 13021191806DNABrassica napus 119atggctcatt gttggacagt tcttaccggt ttactagtat ggtctctttt gatgctctca 60tggtgcgaag cttcaagaaa caacattaat agatatgatc attcttatgg aacgttcaaa 120tccaatagct taatcaagcg aagagacgat atcactcgtc tgaaaagtgt tgcaagagct 180tctttgcggc ttccaaccac cgttagtgtt tctgattttg gagctaaagg agatggaaaa 240accgacgaca cgcaggcgtt cgtgaatgca tggaagaaag cttgttcttc aagtggacct 300gtaaatctct tagttcctga aggaaaaact tattttctca agtctcttcg attaaatggc 360ccatgcaaat cagttcttac cgttcagatt ttaggaacgt tatcggcatc tcaacaacga 420tcggattata aagatctcag caaatggatt acgtttgacg gcgttaacag tctaacggtt 480gatggcgggg ccaccggaac tgtcatggct cattgttgga cagttcttac cggtttacta 540gtatggtctc ttttgatgct ctcatggtgc gaagcttcaa gaaacaacat taatagatat 600gatcattctt atggaacgtt caaatccaat agcttaatca agcgaagaga cgatatcact 660cgtctgaaaa gtgttgcaag agcttctttg cggcttccaa ccaccgttag tgtttctgat 720tttggagcta aaggagatgg aaaaaccgac gacacgcagg cgttcgtgaa tgcatggaag 780aaagcttgtt cttcaagtgg acctgtaaat ctcttagttc ctgaaggaaa aacttatttt 840ctcaagtctc ttcgattaaa tggcccatgc aaatcagttc ttaccgttca gattttagga 900acgttatcgg catctcaaca acgatcggat tataaagatc tcagcaaatg gattacgttt 960gacggcgtta acagtctaac ggttgatggc ggggccaccg gaactgtcaa cggaaacggc 1020gaaacgtggt gggaaaactc atgcaaacgg aacgaagcta agaaatgcac aaaagcccca 1080acggctctta ctttctacaa ctcgaagaat ttgagagtta ataatctgag ggtgaaagat 1140gcgcaacaga tccagatttc gattgagaaa tgctccaacg ttcaggtctc aaatgttgag 1200gtcactgcgc ctgctgatag tcctaacact gatggtatcc acatcactaa cactcaaaac 1260attcaagtct ccaaatccat cattggaaca ggtgatgatt gtatatctat tgaaagtgga 1320tcacaaaacg ttaaaatcaa cgatttaact tgcggccccg gtcacggtat cagcattggg 1380agcttaggag atgacaattc aaaggcattt gtatcaggcg tgactgtgga tggtgctaaa 1440ctttccggta cagacaatgg agttaggatc aaaacttacc agggagggtc tggaactgct 1500agcaacataa tatttcagaa cattcagatg gagaatgtga agaatccaat cataatcgac 1560caagattatt gcgacaagag caaatgcacc gaacaaaaat cagcggtcca aatcaagaac 1620gtggtgtacc ggaacataag cggcacaagc gcatcagaca tagcaataac tttcaactgt 1680agcaagaact atccatgcca aggaattgta ctcgacaaag tgaacattaa gggagggaaa 1740gcgagttgca gcaacgctaa tgtgatggat aaagacggtg ttttgcctca gtgcaaatcc 1800acttga 1806120657DNABrassica napus 120atgggtaatt ccgacgaagg tgatcgtctt cctgctccat cttcttcaga cgaactctcg 60agcattctgc ggcaggtact gtcccgtact cccacagctc aaccttcttt ctcaccgaag 120aaaatcgttt cctccgctga gatgttcaac cgaaccttcc ccctccttat cccaacaccc 180ggcggagcgg tttcttacgc cgcttgtgca gccgctgaaa ctggggaagg caaatgtggt 240ttcgaaaaca agagaaatgg agctaaacag cgaaattcat tgaagagaaa cattgatgca 300cagtttcaca acttgtctga aaagaggagg aggagcaaga tcaatgagaa aatgaaagca 360ttgcagaaac tgatacccaa ttccaacaag acagataagg cctcaatgct tgatgaagct 420atagaatatc tgaaacagct tcaacttcag tttcagacgt tagccgctat gaatggttta 480ggcctaaatc ctctgcgatt accaccaatt ctaccgccta cgcagacaag gatcactgga 540acctctgaac aagggctgaa ccttgagact ctgcttggtg gttctcactc gatggctaac 600catgaaccac ccgaaccaac tcaggaaatg tgcttttcca caaccactct gctttga 657121985DNABrassica napus 121atgggtaatt ccgatgaagg tgatcgtctt cctgctccat cttcttcgga cgaactctcg 60agcattctcc ggcaggtact gtcccgtact cccacagctc aaccttcttt ctcaccgaag 120aaaatcgttt cctccgctga gatgttcaac cgaaccttcc ccctcgttcc cggcggagcg 180gtttcttacg ccgcttgtgc agtcgctgaa actggggaag gcaaatgtgg tttcgaaaac 240aagagaaatg gagctagaca gcgaaattca ttgaagagaa acattgatgc acagttccac 300accttgtctg aaaagaggag gaggagcaag atcaatgggt aattccgatg aaggtgatcg 360tcttcctgct ccatcttctt cggacgaact ctcgagcatt ctccggcagg tactgtcccg 420tactcccaca gctcaacctt ctttctcacc gaagaaaatc gtttcctccg ctgagatgtt 480caaccgaacc ttccccctcg ttcccggcgg agcggtttct tacgccgctt gtgcagtcgc 540tgaaactggg gaaggcaaat gtggtttcga aaacaagaga aatggagcta gacagcgaaa 600ttcattgaag agaaacattg atgcacagtt ccacaccttg tctgaaaaga ggaggaggag 660caagatcaac gagaaaatga aagctttgca gaaactgata cccaattcca acaaggtgac 720agataaggcc tcaatgcttg atgaagctat agaatatctg aaacagcttc aacttcagtt 780tcagacgtta gccgctatga atggtttagg cctaaatcct ctgcgattac caccaattct 840accgcctacg cagacaggga tcactggaac ctcagaacaa gggctgaacc ttgagactct 900gcttggtggt tctcactcga tggctaacct tgaaccaccc gaaccaactc aggaaatgtg 960ctttcccaca accactctgc tttga 985122645DNABrassica napus 122atgggtaatt ccgacgaagg tgatcgtctt cctgctccat cttcttcgga cgaactctcg 60agcattctcc ggcaggtact gtcccgtgct cccacagctc aaccttcttt ctcaccgaag 120aaaatcgttt cctccgctga gatgttcaac cgaaccttcc ccctcgttcc cggcggagcg 180gtttcttacg ccgcttgtgc agccgctgaa actggggaag gcaaatgtgg tttcgaaaac 240aagagaaatg gagctagaca gcgaaattca ttgaagagaa acattgatgc acagttccac 300aacttgtctg aaaagaggag gaggagcaag atcaatgaga aaatgaaagc tttgcagaaa 360ctgataccca attccaacaa gacagataag gcctcaatgc ttgatgaagc tatagaatat 420ctgaaacagc ttcaacttca gtttcagacg ttagccgcta tgaatggttt aggcctaaat 480cctctgcgat taccaccaat tctaccgcct acgcagacaa ggatcactgg aacctcagaa 540caagggctga accttgagac tctgcttggt ggttctcact cgatggctaa ccgtgaacca 600cccgaaactc aggaaatgtg ctttcccaca accactctgc tttga 645123651DNABrassica napus 123atgggtaatt ccgatgaagg tgatcgtctt cctgctccat cttcttcgga cgaactctcg 60agcattctcc ggcaggtact gtcccgtact cccacagctc aaccttcttt ctcaccgaag 120aaaatcgttt cctccgctga gatgttcaac cgaaccttcc ccctcgttcc cggcggagcg 180gtttcttacg ccgcttgtgc agtcgctgaa actggggaag gcaaatgtgg tttcgaaaac 240aagagaaatg gagctagaca gcgaaattca ttgaagagaa acattgatgc acagttccac 300accttgtctg aaaagaggag gaggagcaag atcaacgaga aaatgaaagc tttgcagaaa 360ctgataccca attccaacaa ggtgacagat aaggcctcaa tgcttgatga agctatagaa 420tatctgaaac agcttcaact tcagtttcag acgttagccg ctatgaatgg tttaggccta 480aatcctctgc gattaccacc aattctaccg cctacgcaga cagggatcac tggaacctca 540gaacaagggc tgaaccttga gactctgctt ggtggttctc actcgatggc taaccttgaa 600ccacccgaac caactcagga aatgtgcttt cccacaacca ctctgctttg a 651124645DNABrassica napus 124atgggtaatt ccgacgaagg tggtcgtctt cctgctccat cttcttcaga cgaactctcg 60agcattctgc ggcaggtact gtcccgtact cccacagctc aaccttcttt ctcaccgaag 120aaaatcgttt cctccgctga gatgttcaac cgaacattcc ccctcgttcc cggcggagcg 180gtttcttacg ccgcttgtgc agccgctgaa actggggaag gcaaatgtgg tttcgaaaac 240aagagaaatg gagctaaaca gcgaaattca ttgaagagaa acattgatgc acagtttcac 300aacttgtctg aaaagaggag gaggagcaag atcaatgaga aaatgaaagc tttgcagaaa 360ctgataccca attccaacaa gacagataag gcctcaatgc ttgatgaagc tatagaatat 420ctgaaacagc ttcaacttca gtttcagacg ttagccgcta tgaatggttt aggcctaaat 480cctctgcgat taccaccaat tctaccgcct acgcagacaa ggatcactgg aacctcagaa 540caagggctga accttgagac tctgcttggt ggttctcact cgatggctaa ccgtgaacca 600cccgaaactc aggaaatgtg ctttcccaca accactctgc tttga 645125651DNABrassica napus 125atgggtaatt ccgatgaagg tgatcgtctt cctgctccat cttcttcgga cgaactctcg 60agcattctcc ggcaggtact gtcccgtact cccacagctc aaccttcttt ctcaccgaag 120aaaatcgttt cctccgctga gatgttcaac cgaaccttcc ccctcgttcc cggcggagcg 180gtttcttacg ccgcttgtgc agtcgctgaa actggggaag gcaaatgtgg tttcgaaaac 240aagagaaatg gagctagaca gcgaaattca ttgaagagaa acattgatgc acagttccac 300accttgtctg aaaagaggag gaggagcaag atcaacgaga aaatgaaagc tttgcagaaa 360ctgataccca attccaacaa ggtgacagat aaggcctcaa tgcttgatga agctatagaa 420tatctgaaac agcttcaact tcagtttcag acgttagccg ctatgaatgg tttaggccta 480aatcctctgc gattaccacc aattctaccg cctacgcaga cagggatcac tggaacctca 540gaacaagggc tgaaccttga gactctgctt ggtggttctc actcgatggc taaccttgaa 600ccacccgaac caactcagga aatgtgcttt cccacaacca ctctgctttg a 6511267235DNABrassica napus 126atataagcat gttgagtcct attcatcaga aaattttctc caaaggatga acaataacct 60acaattaagg aaacttatta taacaacgac caagaagttt tcccataagt catgctttcg 120tccatacctt cgggtctttc aagcaagctt cacacatgcc ttatcgtata tttgtatacg 180ttttcatttt gtgtacgtct attaattagt atatgcctag attatatatt tatatagtcg 240acaattacct atcctaaagg tttattttaa tttttattca gaaagatatg cacgtagggc 300cactagtgtt tcccgttgtt attggaaatt ccatgtgttg aactgtttga ttaagttttt 360gaacccgtaa gtcaaattta tttaggttta acagggtttc attactgtta taagcttgtc 420aagaaagaat ttgtcacaaa tttcgagact catgcatgca tgcatgtttc ttaactttcg 480agaaacatga agtgtggttt taccagctga agtttgtgtt aaaacacatt tttaatttga 540ccacgtgtga tattcaaata tcaacaattc tgttcaacgg gagattcata catatgttca 600gatgttcttt aatcttgagc acctaacttt gcttcccata cgcaaatgtg tgtgccgcga 660actatggtga atgtacggca tcgtttttct ttttggagca tttttaaaag aaaatataat 720ttagttattt ccgttttgat tgatatagat gcattaataa accgacttac gtgggtgatg 780ggatgagcaa aattaaaata tgcattttag taaccaatta cggtccatct tgacaaacta 840acaaacaaaa agtctattat gacgttgatt tgtgaccttg ccatataaat gccaaaattt 900cattctttca ataagcaacg agatcaataa gatgtggcag tcacgtgaac gttagttatc 960gccatttcaa ctgaaccttc acgaacatat aaagttcatt tgttctttgc taaaattacg 1020caaatctctg ttgtgcacga aattacgact attttttaat gacaactcct gttcttgtat 1080cgtgtatcta gcacgtaaat ttgaattatg gcggcattat cgataaaata tgtaaagaat 1140atattttcat catacaccac aaacaaatgt tctggaaata tcacctttta tcacatatac 1200aaagctgacc ctgaattttt ggaaactaca agccaaaaaa aaatggacaa aagaaaaatt 1260atgcacaaaa gtggactcgt cctaactctt tttataatga taattaacct ttgaacaact 1320aattaataca tacaatatgt tttttttttc taaaaattgg tcgattaaat attcatatat 1380aatcctcatc atcatttatt agccttttgt agcttactta aaaaatcact tcccaccaaa 1440ttgattattt tttcacttca tacgttgatc aatttaaaaa aaaaataata ataatatatg 1500cacgtccggc gtcccatgac ttcagacgct tttctgtttc cttgatttta taggttagaa 1560atttccaaaa tgctcatatt tttcactgat atgtattata cgcacaaaat agtaaagtca 1620taaaaattta tgatggttct tttatatgta tagagtagtg aaaatttata tatattgtag 1680ttccgcttaa aataaattaa tatttttctt ttatcgccca tatttaagtt actaaacgta 1740tataattaaa aactgaaatg caatatgtag aaatccgggc attgattgtt gattaacatt 1800gatataccca tgcgactggc ttatgatgaa ccatacctgc taatactcat tgttacaaac 1860tcgcagcaac atcataaatc catcactcgc cacaataatt ttcttcagtg tatgttcaat 1920tgatcacatt ttgacccttc actttcttga atgagaaatt atacaagaaa gtgatttgag 1980tgtgttaaaa aatgatttgt cattctgacc aattatctgg cagagagtat aataaaatac 2040ttctaattac cttaaatcat taggcgttgt ttcttgccga atatatgatg gagcatggtc 2100tcgctaagag acaacaaaat ctttaaataa attaaagaaa ttgtttagca aaaaaattaa 2160ataaattaaa caagagagag tacaaatata aaacactcta acggaagata aatatataaa 2220atacaaaata tagaatagag gcctcaagta gtgtttacgt gacaatctca atttcactcc 2280atacctcgaa ctcattcaag cttagaaacc taacttctac tgttcctttt gatttgccta 2340taaatacacg ttggtccata cttctctaat ttcaaatata caaagcaaaa cacagagaca 2400agaatcataa ataacaaaca ttttcaacat aatcccaaaa aacgacacgc gttccttttc 2460caaaaattca cttctctacg taccaaccac aatggctcat tgttgcacag ttcttaccgg 2520tttactagta tggtctcttt ttatgctctc atggtgtgaa gcttcaagaa acaacattaa 2580tagatatgat cattcttatg gaacgttcaa atccaatagc ttaatcaagc gaagagacga 2640tataactcgt ctgaaaagtg ttgcaagagc ctctttgcgg cttccaacca ccgttagtgt 2700ttctgatttt ggagctaaag gagatggaaa aaccgacgac acgcaggtat atacgcatat 2760acacatatct acatgactta taaatatttt gagacggtca cgcatataaa tttacgttgt 2820ttgtactaat gatccatgca tgcgcatgca tatgatcagg cgttcgtgaa tgcatggaag 2880aaagcttgtt cttcaagtgg agctgtaaat ctcttagttc ctgaaggaaa aacttatttt 2940ctcaagtctc ttcgattaaa tggcccatgc aaatcagttc ttaccgttca ggtaaattac 3000aaagtttttt tttcttaaat ttgggtaaat tacaaagttg actatttgtt aattaaatac 3060gtatactaca tgtgatcacg tatatggttt tgaatattta aatgagcggt tgatgaatgg 3120aacgacagat tttaggaacg ttatcggctt ctcaacaacg atcggattat gaagatctca 3180gcaaatggat tacgtttgac ggcgttaaca gtctaacggt tgatggcgga gccaccggaa 3240ctgtcaacgg aaacggcgaa acgtggtggg aaaactcatg caaacggaac gaagctaagg 3300tgtgtgtttg tacatgtttc atttacttaa tatatatcta ctgtctacat gtcatcttga 3360tatattaaca ctcttttatt gttattgttt tgaagaaatg cacaaaagcc cctacggtac 3420gtagaaacat atttgacatt ttcatgtaat actgacgatg gcggtgatca tgattggaca 3480aatgttttac atgcatgaat atatgtattt tatatgcagg ctcttacttt ctacaactcg 3540aagaatttaa gagtgaataa tctgagggtg aaagatgcgc aacagattca gatttcgatt 3600gagaaatgct ccaacgttca ggtctcaaat gttgaggtca ctgcacctgc tgatagtcct 3660aacactgatg gtatccacat cactaacact caaaacattc aagtctccaa ttccatcatt 3720ggaacaggtt agtagttata tcctcgttat ttaaaagagt tgcgtgcata tccagaaaac 3780taaaatttaa gagtatcgtt ataatttata attttataga gattggtctt aaaagagttt 3840ttaatggttg cttttctttg caggtgatga ttgtatatct attgaaagtg gatcacaaaa 3900cgttaaaatc aacgatttaa cttgcggccc aggccacggt atcaggtatc aatgaattgt 3960tttcagtata tgtacctaag aaattcaacc gtaatctttt aaattaacat agaaatttga 4020tatttccata taaaacatga gcagcattgg gagcttagga gatgacaatt caaaggcatt 4080tgtatcaggc gtgactgtgg atggtgctaa actttccggt acagacaatg gagttaggat 4140caaaacttac caggtgaaaa tcacaaacta gaccttcaat tttaactagc ctattgacaa 4200gttcatctca atttaacttt taaacccaat tcaatatagg gagggtctgg aactgctagc 4260aacattatat ttcagaacat tcagatggag aatgtgaaga atccaatcat aattgaccaa 4320gattattgcg acaagagcaa atgcactgaa caagttcgtt tgaatctgat caaatctccc 4380attactaatc atcatgataa taaacactat tgtctctaat catttactac atatattttc 4440agaaatcagc ggtccaaata aagaacgtgg tgtaccggaa cataagcggc acaagcgcat 4500cggacatagc aataactttc aactgcagca agaactatcc atgccaagga attgtactcg 4560acaaagtgaa cattaaggga gggaaagcca gttgcagcaa tgctaatgtg atggataaag 4620acggtgtttt acctcagtgc aaatccactt gattaataag acctataaaa aaaactcaca 4680tagatgtata aaaatacata tataccgata caaagtatac aaaattatga tatacacttg 4740tggatatata catggacaat gaatttaaac atattgtcta tatgtcaaaa atagtttatg 4800aatatacata tagacaacat ggtcaagaga tttattgttt taatgtatat atatactact 4860aaactacttg tctaaaattt catgaataaa ggattacgtt tttaagtggt tatgtatcat 4920gatgtggtta cagtatgatg tgtattgtag attttcaatt aaatacataa aaatgaaaaa 4980gatgacatgg ctggtcaatg atgttacttg accaactaat tcaagctaat tgatacacga 5040tgaacatcta acgaaacaga agcgactttc atattgttta gttgtgctca aaccaggccg 5100ataaactcat tgggcctata agcccattaa tatttatccg tgagagcgaa gtctgttact 5160tgggttacta aacaaagagg cctttagaaa accctaactc ttaccgtagc acctgagcta 5220tatattgccg cagctagggt ttccactccc tcacgctaac acacatcacc gcagccattt 5280ccatctctca accaacttca aacaatggct gaacgaggcg gagaacgagg tgccgagcga 5340ggaggagacc gcggcggttt cggacgcgga ttcggaggag gtcgcggcga cagaggaggc 5400cggggaggtc cgagaggccg aggcggcaga cgcggaggac gtccgacaga ggaggagaag 5460tggaccccag tgaccaagct cggccgtctc gtgcaagccg gaaagatcca gaagctcgag 5520cagatctacc tccactccct ccccgtcaag gagtaccaga tcatcgacct cctcgtcggc 5580cccaccttga aagacgaggt gatgaagatc atgccggttc agaaacagac cagggccggt 5640cagaggacga ggttcaaggc ctttgtcgtc gtcggagaca cgaacgggca cgttggtttg 5700ggtgtgaagt gctccaagga ggtggcgacg gcgatcagag gtgggattat attggcgaag 5760ctgtcggtta ttcccgtgag gagagggtac tgggggaaca agattgggaa gccgcatacg 5820gttccttgta aggttacggg gaagtgtggg tctgttacgg tgaggatggt gcctgctccg 5880aggggtgcgg ggattgtggc ggctagggtt cctaagaagg tgcttcagtt cgctgggatt 5940gatgatgtgt ttacttcgtc gagggggtct actaagactc ttggaaactt cgtaaaggtt 6000tgaactttct tcatttattt agagtttgga gttgtgtatg gattgaaagt tgattccttt 6060ttgatggttg cgtgtggagt tgttttgggt aatttagctt gttgtagttg atgtaaagtg 6120agaattgcta agcttgatta gtttgaataa gctgtctgtg ttaaatgatg atttgtttgt 6180gttgtattga gctgtctgaa tttcattatt tgtttgtgtt attgctaaat tacaagctta 6240aatttgataa cttttgattc tttcgtgaga gttttttttg ggtaatatag cttgatgtag 6300ttgatggaag taaattgtta acctttagag ctgtctgtta atggtgattt gtttgtgttg 6360tattgagctg tctgaattat aatttgtttg tgtttattgc taaatcacaa agctttgaag 6420tcgtttgtac

atattcatca tttttgtgtt tattgacttg tttgtgttta ttgctaaatc 6480acaagtttct atgattaatc ttcatgattt ggttgtgttt attactaaat cacaagtttc 6540tatgattaat cttaatgatt tggttgtgtt tattgctaaa tttgagatta cattataatc 6600ttagtttata catgtataac atcatttcac ttgaattttc acagacgagc tggtgatatg 6660tttttttagt attttccatg tattgctttg taagaacttt taaatgtttg ttttcttcct 6720ttgaagaaca attaaatgtt tgttcagtat ttttgaatca ttcattggtt aatcatgtgt 6780aattttattt ttgtaggcta cattcgactg tctccagaag acttatggat tcctgacacc 6840agagttctgg aaagagacga gctttaaaaa gtcaccgtac caggagtaca ctgatctgct 6900ggccgagaag ggaattgcaa ccaaagccat caccgaggtt gaagaccaag ccgcctccta 6960aataaacaaa caaacaatgt cccccttgtt ttgtttttgg atgatccttt cttgagtttt 7020attttgttcg aacctttagt atatgttgtt tgctggattg ctgctttctt atgttttcat 7080catctccccc aaacaaagtt atttttgctc tagttttttt ttataatcga tggttatgcc 7140ctctagtatt ttttttactt gaatttttca ctaattttct atcaacttcc ctatgttaca 7200ctttctaagg ttgattacat aggtggattg ttgtg 72351277172DNABrassica napus 127gtgacctggc catataaatg ccaaattttc attctttcaa taagcaacga gatcaataag 60atgtggcagt cacgtgaacg ttagttatcg ccatttcaac tgaaccttca cgaacatata 120aagttcattt gttctttgct aaaattacgc aaatctctgt tgtgcacgaa attacgacta 180ttttttaaag acaactcctg tgtcttgtat cgtgtatctc gcacataaaa tttgaattat 240gggggcatta tcgataaaat atataaagaa tatattttca tcatacacca caaacaaatg 300ttctggaaat gtcacatttt atcacagata caaagctgac cctgaatttt tggaaactac 360aagccaaaaa aaaatggtca aaagaaaaat tatgcacaaa ggtggactcg ccctaactct 420ttttataatg ataattaacc tttgaacaac taattaaaga tacatacaat atgttttttt 480tctctaaaaa ttggtcgatt aaatattcat atataatcct tatcatcatt tattagcctg 540ttgtagctta cttaaaaaat cacttcctac caatgttttt aaactcgacg cgaaccggac 600gatttaccgg gtcgctgggt cactgtatcg accgcgagcg aaccgcgggt taataaataa 660actaaaattt aaaataatat atcagctatg taaataaaaa tatagaaact aaaatttaat 720attttctaaa tgttcttata aacataaaat aatagtctgg atgtgtatat ttttatgttt 780aataacattt agaaaatact taactttaat ttttatattt ttattttcat ttgatataca 840aaatatcaaa aaatagttta gattatttat aattttgtct aacaaggtaa gagttatggc 900atctaagaaa atcaagacat aaaatttata aatatttaaa tgtttaatgt gaataataaa 960ttaaatttca aatacaaaat aaaccaaaag tgaaaaatca ttataaaaaa ttatcaataa 1020cagaaataaa ccaactccaa atcacatcaa ctagttttgg ttctgtctac cattgttcac 1080ttttattttc ttcaagtggc atagtgaaat cttcattaaa atctaaaaat cacaaatata 1140aaactgaaaa ggaaaaactt aacttttttt tgtcggtata tcacttctta attagaaatt 1200tagaatataa aacataatct aaaaacataa ttaaaaatta aaaatgaagt aaacgttatt 1260tattggttca accagtggtt caaccggtat cgggttccgg gttctattgg gtttttgcgg 1320gtttctaaat attgtgtttt tcacaaaact caaaccggat ttttttaggg ttgccgggtt 1380taccggttca accgcaggtt cgggtcgggt ttcaaaacac tgcttcccac caaattgatt 1440aatgtagatt gattattttt tcacttcata cgttgatcaa tttttaaaaa gaaataatat 1500atgcacgtcc ggcgtcccat gacttcagac gcttttctgt ttccttgatt ttataggtta 1560gaaatttcca aaatgctcat atttttcact catgtattat acgcacaaaa tagtaaattc 1620ataaaaattt atgatggttc tttatatgta tagagtagtg aaatttatat atattgtagt 1680tccgcttaaa ataaattatt atttttcttt tatcgcccat ttttaagtta ctaaacgtat 1740ataattaaaa actgaaatgc aatatgtaga aatccgggca ttgattgttg attaacattg 1800atgatgatac tcatgcgact ggcttatgat gaaccatact aatactcatt gttacaaact 1860cacagcaaca tcataaatcc atcactcgcc acaataattt tctttagtgt atgttcaatt 1920tcacattttg acccttcact ttcttgaatg aaaaattata caagaaagtg atttgagtgt 1980gttagaaaaa aaaaagattt gagtcattct gaccaattat ctgggagaga ctataataaa 2040atacttccaa ttaccttaaa tcatcaggcg ttgtttcttg cctaatatat gatggagcat 2100ggtctcgcta agagacaaca aaatctttaa ataaattaaa caagttgttt agcaaaaata 2160aaaaaaataa attaaacaag agaaagtaca aatataaaac tctaaaggaa gataaatgta 2220taaaatacaa gatatagaat agaagcgttt acgtgacaat ctcaatttca ctccatacct 2280cgaactcatt caagcttaga aacctaactt ttactgttcc ttttgatttg cctataaata 2340cacgttcgtc catacttctc taatttcaaa tatacaaagc aaaacacaga gacaagaatc 2400ataaacaaca aacattttca acataatccc aaaaagacga cacgcattcc ttcttccaaa 2460aatccacttc ctacgtacca accacaatgg ctcattgttg cacagttctt accggtttac 2520tagtatggtc tcttttgatg ctctcatggt gcgaagcttc aagaaacaac attaatagat 2580atgatcattc ttatggaacg ttcaaatcca atagcttaat caagcgaaga gacgatatca 2640ctcgtctgaa aagtgttgca agagcctctt tgcagcttcc aaccaccgtt agtgtttctg 2700attttggagc taaaggagat ggaaaaaccg acgacacgca ggtatatacg catacacaca 2760tatctacatg acttataaat attttgagac ggtcatgtat ataaatttac gttatttgta 2820ctaatgatcc atgcatgcat gcatatgatc aggcgttcgt gaatgcatgg aagaaagctt 2880gttcttcaag tggagctgta aatctcttag ttcctgaagg aaaaacttat ttccttaagt 2940ctattcgatt aaatggccca tgcaaatcaa ttcttaccgt tcaggtaaat tacaaagttg 3000acaaaaaaac aattacaaag ttgactattt gttaattata tacgtatact acatgtgatc 3060acgtatatgg ttttgaatat ataaatgagc ggttgacgaa tggaatgaca gattttagga 3120acgctatcgg catctcaaca acgatcggat tataaagatc tcagcaaatg gattacgttt 3180gacggcgtta acagtctaac ggttgatggc ggggccaccg gaactgtcaa cggaaacggc 3240gaaacgtggt gggaaaactc atgcaaacgg aacgaagcta aggtgtgtgt ctgtacatgt 3300ttcatttact taatatatat ctactattta catgtcatct tgatatattg acacttttta 3360ttgttattgt tattgttttc aagaaatgca caaaagcccc aacggtacgt agaaacatat 3420ttgacatttt catgtaatac tgacgatggc tgtgatcatg attggacaaa tgttttacat 3480gcatgaatat atgtatttta tatgcaggct cttactttct acaactcaaa gaatttgaga 3540gttaataatc tgagggtgaa agatgcgcaa cagatccaga tttcgattga gaaatgctcc 3600aacgttcagg tctcaaatgt tgaggtcact gcgcctgctg atagtcctaa cactgatggt 3660atccacatca ctaacactca aaacattcaa gtctccaaat ccatcattgg aacaggttag 3720taattacatc ctcgttattt aaaagagttg cgtgcatatc cagaaaacta atatttaagg 3780gtttatcgtt ataatttata gagattggtc ttaaaagagt tttttaatgg ttgcttttct 3840atgcaggtga tgattgtata tctattgaaa gtggatcaca aaacgttaaa atcaacgatt 3900taacttgcgg ccccggtcac ggtatcaggt atcaatgaat tgttttcagt atatttacct 3960aagaaattca accgtaatct tttacatata aatttgaaat ttccttataa aacatgagca 4020gcattgggag cttaggagat gacaattcaa aggcatttgt atcaggcgtg actgtggatg 4080gtgctaaact ttccggtaca gacaatggag ttaggatcaa aacttaccag gtgaaaatca 4140caaactaaac cttcaatttt aactagccta tcaagttcat ctcaatttaa cttttaaacc 4200caattcaata tagggagggt ctggaactgc tagcaacatt atatttcaga acattcagat 4260ggaaaatgtg aagaatccaa ttataatcga ccaagattat tgcgacaaga gcaaatgcac 4320tgaacaagtt cgtttgaatc tgatcaaatc ttccattact aagcgtcata ataatataaa 4380cttttctcta atcatttact acatattttc agaaatcagc ggtccaaata aagaacgtgg 4440tgtaccggaa cataagcggc acaagcgcat cggacatagc aataactttc aactgcagca 4500agaactatcc atgccaagga attgtagtcg acaaagtgaa cattaaggga gggaaagcaa 4560gttgcagcaa cgctaatgtg atggataaag acggtgtttt gcctcagtgc aaatccactt 4620gattaactag acctatcaaa aactcacata gatgtataaa tacatataga ccgatacaaa 4680gtatacaaaa ttatgatata tacttgtgga tatatacatg gacaatgaat ttaaacatat 4740tgtttatatg tcaaaatagt ttatgaatag acatataaac aacatgttca agagatttat 4800tgtcttaatg tatatactgc atactaaatt gtttgtctaa aacttcatat aataaaaact 4860gttaatcttc atgatggtgt gatttgttgt gataacagta tgatgtctat atttgtaaaa 4920aaaaaaacag tatgatgtct attgtagatt tttatctaaa tacataaaaa ttgaaaaaaa 4980aaaaatgaca tggctacttg accgattgat tcaagttaat tgttactaac caggccgata 5040aacaaattgg gcctttaaag cccgttaata tttatcagtg aaagcgaagt ctgttacttg 5100ggttactaaa caaaggcctt tagaaaaccc taactcttat cgcagcacct gagctatata 5160ttgccgcagc tagggttttc gctagctcac gctaacacac aaagccgcag ccatttccat 5220ctctctcgca actcaacttc gaacaatggc tgaacgaggc ggagaacgag gcgccgagcg 5280aggaggagac cgcggcggtt tcggacgggg attcggagga ggtcgcggcg acagaggagg 5340ccgcggaggt ccgagaggcc gaggcggcag acgcggagga cgtcccacag aggaggagaa 5400gtggacccca gtgaccaagc tcggccgtct cgtgcaagcc ggaaagatcc agaagctcga 5460gcagatatac ctccactccc tccccgtcaa ggagtaccag atcatcgacc tcctcgtcgg 5520tccaacttta aaagacgagg tgatgaagat catgccggtt cagaaacaga ccagggccgg 5580tcagaggacg aggttcaagg cttttgtcgt tgtaggagac acgaatggtc atgttggttt 5640gggtgtgaag tgctccaagg aggtggcgac ggcgatcagg ggtgggatta tattggcgaa 5700gctgtctgtg attcccgtga ggagagggta ctgggggaac aagattggga agccgcatac 5760ggttccttgt aaggttacgg ggaagtgtgg gtctgttacg gtgaggatgg tgcctgctcc 5820gaggggggct gggattgtgg cggctagggt tcctaagaag gtgcttcagt tcgctgggat 5880tgatgatgtg tttacttcct cgagggggtc tactaagact cttggaaact tcgtaaaggt 5940ttgaacttta ttattttact tggtgtttgg agttgtgtat ggattgaaag ttgattcctt 6000tttgatcgtt gcttgtggag gtgttttggg taatattgct aaatgtaaaa attgccaagc 6060ttgattagtt tgaataagct gtctgtgtta aatgatgatt tgtttgtgtt gtaatgagct 6120gtctgaattt cattatttgt ttgtgttatt gctaatttac aagcttaaat ttgattcctt 6180ttgatcgttt cgtgtgagtt gttttgtgta atttagcttg atgtagttga tggaagtaag 6240aattgttaag ctttagagct gtctgttaat gttgaattgt ttgtgttgta ttgagctgtc 6300tgaattataa tttgtttgcg atttattgct aaatcacaaa gctctaaagt cgtttgaaca 6360tatgcatcat ttggttagtg tttattgctg aattttcatg atttggttgt gtttattgct 6420aaatttgaga ttacattata atcttaattt gtacatatat tcatcatttt tgtgtttatt 6480gatttgtttg tgtttattga tgaatcacaa gtttctatga ttaatcttca tgatttggtt 6540gtgattattg ctaaatttga gattacatta taagcttagt ttgtacatgt atacatcatt 6600tcacttacat ttcacttggt ggtgatatat attttttttt tagtattttc catgtattgc 6660tttgtaagaa cgtttaaatg ttagttttct tgaatgtttt tttctttgta agaacaatta 6720aatgtttgtt cagtattttt gaatcattca atggttaatc atgttcaaca tgtatttttt 6780gtaggctaca tttgactgtc tccagaagac ttatggattc ctgacaccag agttctggaa 6840agagacgagc tttaaaaagt caccgtacca ggagtacact gatttgctgg ccgagaaggg 6900aactgcaacc aaagccatca ccgaggttga agaccaagcc gcctcctaaa taaacaaaca 6960agcaatgtcc accttgtttt gtttttggat gatcctttca tgagttttat tttgttcgaa 7020ccttttagta tatgttgttt gctggattgc tgctatctta agttttcatc atcttcccaa 7080acaaagttat ttttgctcta gttttttttt ttttaataat cgatggttat actctagtat 7140ttttttactt gaatttctct aattttctat ca 71721287239DNABrassica napus 128ataatataag catgttgagt cctattcatc agaaaatttt ctccaaagga tgaacaataa 60cctacaatta aggaaactta ttataacaac gaccaagaag ttttcccata agtcatgctt 120tcgtccatac cttcgggtct ttcaagcaag cttcacacat gccttatcgt atatttgtat 180acgttttcat tttgtgtacg tctattaatt agtatatgcc tagattatat atttatatag 240tcgacaatta cctatcctaa aggtttattt taatttttat tcagaaagat atgcacgtag 300ggccactagt gtttcccgtt gttattggaa attccatgtg ttgaactgtt tgattaagtt 360tttgaacccg taagtcaaat ttatttaggt ttaacagggt ttcattactg ttataagctt 420gtcaagaaag aatttgtcac aaatttcgag actcatgcat gcatgcatgt ttcttaactt 480tcgagaaaca tgaagtgtgg ttttaccagc tgaagtttgt gttaaaacac atttttaatt 540tgaccacgtg tgatattcaa atatcaacaa ttctgttcaa cgggagattc atacatatgt 600tcagatgttc tttaatcttg agcacctaac tttgcttccc atacgcaaat gtgtgtgccg 660cgaactatgg tgaatgtacg gcatcgtttt tctttttgga gcatttttaa aagaaaatat 720aatttagtta tttccgtttt gattgatata gatgcattaa taaaccgact tacgtgggtg 780atgggatgag caaaattaaa atatgcattt tagtaaccaa ttacggtcca tcttgacaaa 840ctaacaaaca aaaagtctat tatgacgttg atttgtgacc ttgccatata aatgccaaaa 900tttcattctt tcaataagca acgagatcaa taagatgtgg cagtcacgtg aacgttagtt 960atcgccattt caactgaacc ttcacgaaca tataaagttc atttgttctt tgctaaaatt 1020acgcaaatct ctgttgtgca cgaaattacg actatttttt aatgacaact cctgttcttg 1080tatcgtgtat ctagcacgta aatttgaatt atggcggcat tatcgataaa atatgtaaag 1140aatatatttt catcatacac cacaaacaaa tgttctggaa atatcacctt ttatcacata 1200tacaaagctg accctgaatt tttggaaact acaagccaaa aaaaaatgga caaaagaaaa 1260attatgcaca aaagtggact cgtcctaact ctttttataa tgataattaa cctttgaaca 1320actaattaat acatacaata tgtttttttt ttctaaaaat tggtcgatta aatattcata 1380tataatcctc atcatcattt attagccttt tgtagcttac ttaaaaaatc acttcccacc 1440aaattgatta ttttttcact tcatacgttg atcaatttaa aaaaaaaata ataataatat 1500atgcacgtcc ggcgtcccat gacttcagac gcttttctgt ttccttgatt ttataggtta 1560gaaatttcca aaatgctcat atttttcact gatatgtatt atacgcacaa aatagtaaag 1620tcataaaaat ttatgatggt tcttttatat gtatagagta gtgaaaattt atatatattg 1680tagttccgct taaaataaat taatattttt cttttatcgc ccatatttaa gttactaaac 1740gtatataatt aaaaactgaa atgcaatatg tagaaatccg ggcattgatt gttgattaac 1800attgatatac ccatgcgact ggcttatgat gaaccatacc tgctaatact cattgttaca 1860aactcgcagc aacatcataa atccatcact cgccacaata attttcttca gtgtatgttc 1920aattgatcac attttgaccc ttcactttct tgaatgagaa attatacaag aaagtgattt 1980gagtgtgtta aaaaatgatt tgtcattctg accaattatc tggcagagag tataataaaa 2040tacttctaat taccttaaat cattaggcgt tgtttcttgc cgaatatatg atggagcatg 2100gtctcgctaa gagacaacaa aatctttaaa taaattaaag aaattgttta gcaaaaaaat 2160taaataaatt aaacaagaga gagtacaaat ataaaacact ctaacggaag ataaatatat 2220aaaatacaaa atatagaata gaggcctcaa gtagtgttta cgtgacaatc tcaatttcac 2280tccatacctc gaactcattc aagcttagaa acctaacttc tactgttcct tttgatttgc 2340ctataaatac acgttggtcc atacttctct aatttcaaat atacaaagca aaacacagag 2400acaagaatca taaataacaa acattttcaa cataatccca aaaaacgaca cgcgttcctt 2460ttccaaaaat tcacttctct acgtaccaac cacaatggct cattgttgca cagttcttac 2520cggtttacta gtatggtctc tttttatgct ctcatggtgt gaagcttcaa gaaacaacat 2580taatagatat gatcattctt atggaacgtt caaatccaat agcttaatca agcgaagaga 2640cgatataact cgtctgaaaa gtgttgcaag agcctctttg cggcttccaa ccaccgttag 2700tgtttctgat tttggagcta aaggagatgg aaaaaccgac gacacgcagg tatatacgca 2760tatacacata tctacatgac ttataaatat tttgagacgg tcacgcatat aaatttacgt 2820tgtttgtact aatgatccat gcatgcgcat gcatatgatc aggcgttcgt gaatgcatgg 2880aagaaagctt gttcttcaag tggagctgta aatctcttag ttcctgaagg aaaaacttat 2940tttctcaagt ctcttcgatt aaatggccca tgcaaatcag ttcttaccgt tcaggtaaat 3000tacaaagttt ttttttctta aatttgggta aattacaaag ttgactattt gttaattaaa 3060tacgtatact acatgtgatc acgtatatgg ttttgaatat ttaaatgagc ggttgatgaa 3120tggaacgaca gattttagga acgttatcgg cttctcaaca acgatcggat tatgaagatc 3180tcagcaaatg gattacgttt gacggcgtta acagtctaac ggttgatggc ggagccaccg 3240gaactgtcaa cggaaacggc gaaacgtggt gggaaaactc atgcaaacgg aacgaagcta 3300aggtgtgtgt ttgtacatgt ttcatttact taatatatat ctactgtcta catgtcatct 3360tgatatatta acactctttt attgttattg ttttgaagaa atgcacaaaa gcccctacgg 3420tacgtagaaa catatttgac attttcatgt aatactgacg atggcggtga tcatgattgg 3480acaaatgttt tacatgcatg aatatatgta ttttatatgc aggctcttac tttctacaac 3540tcgaagaatt taagagtgaa taatctgagg gtgaaagatg cgcaacagat tcagatttcg 3600attgagaaat gctccaacgt tcaggtctca aatgttgagg tcactgcacc tgctgatagt 3660cctaacactg atggtatcca catcactaac actcaaaaca ttcaagtctc caattccatc 3720attggaacag gttagtagtt atatcctcgt tatttaaaag agttgcgtgc atatccagaa 3780aactaaaatt taagagtatc gttataattt ataattttat agagattggt cttaaaagag 3840tttttaatgg ttgcttttct ttgcaggtga tgattgtata tctattgaaa gtggatcaca 3900aaacgttaaa atcaacgatt taacttgcgg cccaggccac ggtatcaggt atcaatgaat 3960tgttttcagt atatgtacct aagaaattca accgtaatct tttaaattaa catagaaatt 4020tgatatttcc atataaaaca tgagcagcat tgggagctta ggagatgaca attcaaaggc 4080atttgtatca ggcgtgactg tggatggtgc taaactttcc ggtacagaca atggagttag 4140gatcaaaact taccaggtga aaatcacaaa ctagaccttc aattttaact agcctattga 4200caagttcatc tcaatttaac ttttaaaccc aattcaatat agggagggtc tggaactgct 4260agcaacatta tatttcagaa cattcagatg gagaatgtga agaatccaat cataattgac 4320caagattatt gcgacaagag caaatgcact gaacaagttc gtttgaatct gatcaaatct 4380cccattacta atcatcatga taataaacac tattgtctct aatcatttac tacatatatt 4440ttcagaaatc agcggtccaa ataaagaacg tggtgtaccg gaacataagc ggcacaagcg 4500catcggacat agcaataact ttcaactgca gcaagaacta tccatgccaa ggaattgtac 4560tcgacaaagt gaacattaag ggagggaaag ccagttgcag caatgctaat gtgatggata 4620aagacggtgt tttacctcag tgcaaatcca cttgattaat aagacctata aaaaaaactc 4680acatagatgt ataaaaatac atatataccg atacaaagta tacaaaatta tgatatacac 4740ttgtggatat atacatggac aatgaattta aacatattgt ctatatgtca aaaatagttt 4800atgaatatac atatagacaa catggtcaag agatttattg ttttaatgta tatatatact 4860actaaactac ttgtctaaaa tttcatgaat aaaggattac gtttttaagt ggttatgtat 4920catgatgtgg ttacagtatg atgtgtattg tagattttca attaaataca taaaaatgaa 4980aaagatgaca tggctggtca atgatgttac ttgaccaact aattcaagct aattgataca 5040cgatgaacat ctaacgaaac agaagcgact ttcatattgt ttagttgtgc tcaaaccagg 5100ccgataaact cattgggcct ataagcccat taatatttat ccgtgagagc gaagtctgtt 5160acttgggtta ctaaacaaag aggcctttag aaaaccctaa ctcttaccgt agcacctgag 5220ctatatattg ccgcagctag ggtttccact ccctcacgct aacacacatc accgcagcca 5280tttccatctc tcaaccaact tcaaacaatg gctgaacgag gcggagaacg aggtgccgag 5340cgaggaggag accgcggcgg tttcggacgc ggattcggag gaggtcgcgg cgacagagga 5400ggccggggag gtccgagagg ccgaggcggc agacgcggag gacgtccgac agaggaggag 5460aagtggaccc cagtgaccaa gctcggccgt ctcgtgcaag ccggaaagat ccagaagctc 5520gagcagatct acctccactc cctccccgtc aaggagtacc agatcatcga cctcctcgtc 5580ggccccacct tgaaagacga ggtgatgaag atcatgccgg ttcagaaaca gaccagggcc 5640ggtcagagga cgaggttcaa ggcctttgtc gtcgtcggag acacgaacgg gcacgttggt 5700ttgggtgtga agtgctccaa ggaggtggcg acggcgatca gaggtgggat tatattggcg 5760aagctgtcgg ttattcccgt gaggagaggg tactggggga acaagattgg gaagccgcat 5820acggttcctt gtaaggttac ggggaagtgt gggtctgtta cggtgaggat ggtgcctgct 5880ccgaggggtg cggggattgt ggcggctagg gttcctaaga aggtgcttca gttcgctggg 5940attgatgatg tgtttacttc gtcgaggggg tctactaaga ctcttggaaa cttcgtaaag 6000gtttgaactt tcttcattta tttagagttt ggagttgtgt atggattgaa agttgattcc 6060tttttgatgg ttgcgtgtgg agttgttttg ggtaatttag cttgttgtag ttgatgtaaa 6120gtgagaattg ctaagcttga ttagtttgaa taagctgtct gtgttaaatg atgatttgtt 6180tgtgttgtat tgagctgtct gaatttcatt atttgtttgt gttattgcta aattacaagc 6240ttaaatttga taacttttga ttctttcgtg agagtttttt ttgggtaata tagcttgatg 6300tagttgatgg aagtaaattg ttaaccttta gagctgtctg ttaatggtga tttgtttgtg 6360ttgtattgag ctgtctgaat tataatttgt ttgtgtttat tgctaaatca caaagctttg 6420aagtcgtttg tacatattca tcatttttgt gtttattgac ttgtttgtgt ttattgctaa 6480atcacaagtt tctatgatta atcttcatga tttggttgtg tttattacta aatcacaagt 6540ttctatgatt aatcttaatg atttggttgt gtttattgct aaatttgaga ttacattata 6600atcttagttt atacatgtat aacatcattt cacttgaatt ttcacagacg agctggtgat 6660atgttttttt agtattttcc atgtattgct ttgtaagaac ttttaaatgt ttgttttctt 6720cctttgaaga acaattaaat gtttgttcag tatttttgaa tcattcattg gttaatcatg 6780tgtaatttta tttttgtagg ctacattcga ctgtctccag aagacttatg gattcctgac 6840accagagttc tggaaagaga cgagctttaa aaagtcaccg taccaggagt acactgatct 6900gctggccgag aagggaattg caaccaaagc catcaccgag gttgaagacc aagccgcctc

6960ctaaataaac aaacaaacaa tgtccccctt gttttgtttt tggatgatcc tttcttgagt 7020tttattttgt tcgaaccttt agtatatgtt gtttgctgga ttgctgcttt cttatgtttt 7080catcatctcc cccaaacaaa gttatttttg ctctagtttt tttttataat cgatggttat 7140gccctctagt atttttttta cttgaatttt tcactaattt tctatcaact tccctatgtt 7200acactttcta aggttgatta cataggtgga ttgttgtga 72391297200DNABrassica napus 129ctggtcacac acaaaaaata atataagcat gttgaattct attcatcaaa gaattttctt 60caaaggatga acaataacct ataattagga aacattataa caacgagcag gaagttttcc 120cagaagtcat gccttcgtcc ataccttcgg gtctttcaag caagcttcac acatgcctta 180tcatatgttt gtatacgttt tcattttgtg tacgtctatt aactagctag tatatgccaa 240gattatatat tttatatagt cgacaattac ctatcctaaa agttcatttt aatttttatt 300cagaaagata tgcacgtagg gccactagtg tttcccgttg ttattggaaa ttcatttcca 360tgtgttgaat tgtttgttta agtttttgaa cccgtaagtc aaatttattt tggtttaaca 420gggtttcatt actgttataa gcttgtcaag aaagaatttg ttacaaattt cgaggctcat 480gcatgcatga ttcttaactt tctagaaaca tgaaatgtgg ttttaccagc tcaagcttgt 540gttaaaacac atttttaatt tgaccacgtg tgatattcaa atatcaacaa ttctgttcaa 600cgggagattc atacatatgt tcagatgttc tttaatctta agcacctaac tttgcttccc 660atacgcaaat gtgtgtgccg cgaactatgg tgaaatgttt tctttttgga gcatttttaa 720aagaaaatat aatttactta tttccgtttt gattgatata catgcattaa taaaccgact 780tacgtgggtg atgggatgag caaaattaaa atatgcattt tagtaaccaa ttacggtcta 840tcttgacaaa ctaacaaaca aaaagtctat tatgacgttg atttgtgacc tggccatata 900aatgccaaaa tttcattctt tcaataagca acgagatcaa taagatgtgg cagtcacgtg 960aacgttagtt atcgccattt caactgaacc ttcacgaaca tataaagttc atttgttctt 1020tgctaaaatt acgcaaatct ctgttgtgca cgaaattacg actatttttt aaatacaact 1080cctgttcttg tatcgtgtat ctctcgcacg taaatttgaa ttatgggggc attatcgata 1140aaatatgtaa agaatatatt ttcatcatac accacaaaca aatgttctgg aaatgtcacc 1200ttttatcaca tatacaaagc tgaccctgaa attttggaaa ctacaagcca aaaaaaatgg 1260tcaaaagaaa tattatgcac aaaggttgga ctcgtcctaa ctctttttac aatgataatt 1320aacctttgaa caactaatta aagatacata caatatgttt tttttctaaa aattggtcga 1380ttaaatattc atatataatc cttatcatca tttattagcc ttttgtagct tacttaaaaa 1440accccttccc accaaattga ttagtgtaga ttgattattt ttcacttcat acgttgatca 1500attttaaaat atatatatat atatatatat atatatatat gcacgtccgg cgtcccatga 1560cttcagacgc ttttctgttt ccttgatttt ataggttaaa attttccaaa atgctcatat 1620ttttcactca tatgtataat gtattattat acgcacaaaa tagtaaagtc ataaacattt 1680atgatggctc ttttatatgt atagagtagt gaaatttata tatatcggag ttccgcttaa 1740aataaattaa tattgttctt ttatcgccca ttttttagtt actaaacgta tataattaaa 1800aactgaaatg caatatgtag aaatccggct tatgatgaac catactaata ctcattgtta 1860caaactcaca gcaacatcat aattccatca ctcgccacaa taattttctt tagtgtatgt 1920tctatttcac attttgaccc ttcactttct tgaatgaaaa ttatacaaga aaatgatttg 1980agtgtgttaa aaaaaaaaat gttttgagtc attctgacca attatctggc agagactata 2040ataaaatact tccaattacc ttaaatcatt aggcgttgtt tcttgccgaa tatatgatgg 2100agcatggtct cgctaagaga caacaaaatc tttaaataaa ttaaacaagt tgtttagcaa 2160aaaataaaaa ataaattaaa caagagagag tacaaatata aaactctaaa ggaagataaa 2220tgtataaaat acaaaatata gaatagaggc cttaagtagt gtttacgtga caatctcaat 2280ttcactccat acctcgaact cattcaagct tagaaaccta acttttactg ttccttttga 2340tttgcctata aatacacgtt catccatact tctgtaattt caaataacaa agcaaaacac 2400aaagataaga atcacaaaca acaaacattc tcaacctaat cccaaaaaga cgacacgcat 2460tcctttttcc aaaaatccac ttcctacgta ccaaccacaa tggctcattg ttggacagtt 2520cttaccggtt tactagtatg gtctcttttg atgctctcat ggtgcgaagc ttcaagaaac 2580aacattaata gatatgatca ttcttatgga acgttcaaat ccaatagctt aatcaagcga 2640agagacgata tcactcgtct gaaaagtgtt gcaagagctt ctttgcggct tccaaccacc 2700gttagtgttt ctgattttgg agctaaagga gatggaaaaa ccgacgacac gcaggtatat 2760acgcatacac acatatctac atgacttata agttataaat attgtgagac ggtcacgtat 2820ataaatttac gttgtttgta ctaatgatcc atgcatgcgc atgcatatga tcaggcgttc 2880gtgaatgcat ggaagaaagc ttgttcttca agtggacctg taaatctctt agttcctgaa 2940ggaaaaactt attttctcaa gtctcttcga ttaaatggcc catgcaaatc agttcttacc 3000gttcaggtaa attacaaagt ttcttttgtt ttgcttaaat ttgggtaaat tacaaagttg 3060acaaaaaaat tacaaagttg actatttgtt aattatatac gtatactaca tgtgatcacg 3120tatatggttt tgaatatatt aaagagcggt tggtgaatgg aacgacagat tttaggaacg 3180ttatcggcat ctcaacaacg atcggattat aaagatctca gcaaatggat tacgtttgac 3240ggcgttaaca gtctaacggt tgatggcggg gccaccggaa ctgtcaacgg aaacggcgaa 3300acgtggtggg aaaactcatg caaacggaac gaagctaagg tgtgtgtctg tacatgtttc 3360atttacttaa tatatatcta ctatctacat gtcatcttga tatattgaca cttttttatt 3420gttattgttt tgaagaaatg cacaaaagcc ccaacggtac gtagaaacat atttgacatt 3480ttcatgtaat actgacgatg gctgtgatca tgattggaca aatgttttac atgcatgaat 3540atatgtattt tatatgcagg ctcttacttt ctacaactcg aagaatttga gagttaataa 3600tctgagggtg aaagatgcgc aacagatcca gatttcgatt gagaaatgct ccaacgttca 3660ggtctcaaat gttgaggtca ctgcgcctgc tgatagtcct aacactgatg gtatccacat 3720cactaacact caaaacattc aagtctccaa atccatcatt ggaacaggtt agtaattaca 3780tcctcgttat ttaaaagagt tgcgtgcata tccagaaaac taatatttaa gggtttatcg 3840ttataattta tagagattgg tcttaaaaga gttttttaat gtttgctttt ctatgcaggt 3900gatgattgta tatctattga aagtggatca caaaacgtta aaatcaacga tttaacttgc 3960ggccccggtc acggtatcag gtatcaatga attgttttca gtaaatatac ctaagaaata 4020caaccgtaat cttttacata taaatttgaa atttccttat aaaacatgag cagcattggg 4080agcttaggag atgacaattc aaaggcattt gtatcaggcg tgactgtgga tggtgctaaa 4140ctttccggta cagacaatgg agttaggatc aaaacttacc aggtgaaaat cacaaactaa 4200accttcaatt ttaactagcc tatcaagttc atcccaattt aacttttaaa cccaattcaa 4260tatagggagg gtctggaact gctagcaaca taatatttca gaacattcag atggagaatg 4320tgaagaatcc aatcataatc gaccaagatt attgcgacaa gagcaaatgc accgaacaag 4380ttcgtttgaa cctaatcaaa tcttccatta ctaatcctca tgataataaa cacttttatc 4440tctaatcatt tactacatat attttcagaa atcagcggtc caaatcaaga acgtggtgta 4500ccggaacata agcggcacaa gcgcatcaga catagcaata actttcaact gtagcaagaa 4560ctatccatgc caaggaattg tactcgacaa agtgaacatt aagggaggga aagcgagttg 4620cagcaacgct aatgtgatgg ataaagacgg tgttttgcct cagtgcaaat ccacttgatt 4680aactagacct atcaaaaact cacacagatg tataaataca tatagaccga tacaaagtat 4740acaaaattat gatatatact tgtggatata tacatggaca atgaatttaa acatattatt 4800tatatgtcaa aatagtttat gaatagacat ataaacaaca tgttcaagag atttattgtc 4860ttaatgtata tactccatac taaattgttt gtctaaaact tcatataata aaaaactgtt 4920aatcttcatg atggtgtgat ttgttgtgat aacagtatga tgtctattgt agatttttat 4980ttaaatacat aaaaattgaa agaaaaaaaa tgacatggct acttgaccaa ttgattcaag 5040ttaattgtta ctaaccaggc cgataaacta attgggcctt taaagcccat taatatttgt 5100cagtggaagc gaagtctgtt acttgggtta caaaacaaag gcctttagaa aaccctaact 5160cttatcgcag cacctgagct atatattgcc gcagctaggg ttttccctag ctcacgctaa 5220cacacaaagc cgcagccatt tccatctctc tcgcaactca acttcgaaca atggctgaac 5280gaggcggaga acgaggcgcc gagcgaggag gagaccgcgg cggtttcgga cggggattcg 5340gaggaggtcg cggcgaccga ggaggccgcg gaggtccgag aggccgaggc ggcagacgcg 5400gaggacgtcc cacagaggag gagaagtgga ccccagtgac caagctcggc cgtctcgtgc 5460aagccggaaa gatccagaag ctcgagcaga tctacctcca ctcgctcccc gtcaaggagt 5520accagatcat cgacctcctc gtcggtccaa ctttaaaaga cgaggtgatg aagatcatgc 5580cggttcagaa acagaccagg gccggtcaga ggacgaggtt caaggctttt gtcgttgtcg 5640gagacacgaa tggtcatgtt ggtttgggtg tgaagtgctc caaggaggtg gcgacggcga 5700tcagaggtgg gattatattg gcgaagctgt cggtgattcc cgtgaggaga gggtactggg 5760ggaacaagat tgggaagccg catacggttc cttgtaaggt tacggggaag tgtgggtctg 5820ttacggtgag gatggtgcct gctccgaggg gggcggggat tgtggcggct agggttccta 5880agaaggtgct tcagttcgct gggattgatg atgtgtttac ttcttccagg ggatctacta 5940agactcttgg aaacttcgta aaggtttgaa ctttattcat ttacttggtg tttggagctg 6000tgtatagatt gaaagttgat tcctttttga tcgttgcgtg tggaggtgtt ttgggtaata 6060ttgctaaatg taaaaattgc taagcttgat tagtttgaat aagctgtctg tgttaaatga 6120tgatttgttt gtgttgtaat gagctgtctg aatttcatta tttgtttgtg ttattgctaa 6180tttacaagct taaatttgat tccttttgat cgtttcgtgt gagttgtttt gtgtaattta 6240gcttgatgta gttgatggaa gtaagaattg ttaagcttta gagctgtctg ttaatgttga 6300attgtttgtg ttgtattgag ctgtctgaat tattatttgt ttgcgattta tttctaaatc 6360acaaagctct aaagtcgttt gaacatatgc atcatttggt tagtgtttat tgctgaattt 6420tcatgatttg gttgtgttta ttgctaaatt tgagattaca ttataatctt aatttgtaca 6480tatattcatc atttttgtgt ttattgattt gtttgtgttt attgatgaat cacaagtttc 6540tatgattaat cttcatgatt tggttgtgat tattgctaaa tttgagatta cattataagc 6600ttagtttgta catgtataca tcatttcact tgaattttca cagacgagct ggtgatatat 6660tttttttttt agtattttcc atgtattgct ttgtaagaac gtttaaatgt tagttttctt 6720gaatgttttt ttctttgtaa gaacaattaa atgtttgttc agtatttttg aatcattcat 6780tggttaatca tgttcaacat gtattttttg taggctacat ttgactgtct ccagaagact 6840tatggattcc tgacaccaga gttctggaaa gagacgagct ttaaaaagtc accgtaccag 6900gagtacactg atctgctggc cgagaaggga actgcaacca aagccatcac cgaggttgaa 6960gaccaagccg cctcctaaat aaacaaacaa gcaatgccca ccttgttttg tttttggatg 7020atcctttcat gagttttatt ttgttcgaac cttttagtat atgttgtttg ctggattgct 7080gctatcttaa gttttcatca tcttcccaaa caaagttatt tttgctctag tttttttttt 7140ttataatcga tggttatact ctagtatttt tttacttgaa tttctctaat tttccatcag 720013020001DNABrassica napus 130tgcggaggtt tttgaaattc gcaaagtcgt tccagaacat caatgttcaa tagacgaacg 60tgggggttac gaaacacaag ctacagctgc ggtaattggt gagcttctga aaaacaagtt 120tggggcgaca ggatcaggtc ccaaacctcg ggagatccgt cacatgatgc gtggtgacct 180aaacgtcaat atatcatact ggaaggcatg gcggtcgagg gacgtcgcaa tggagaacac 240gaaagactca gatgtgactg cttacgcaaa actcccagat taccttaaca agctagtgaa 300gtctaatcca ggttctctcg tcgaactctg tacagaacca cagcaaaatg gtggccaccg 360tttcaaatat ttatttgttg cattggaggc ttctatcaag ggatacgagt acatgaggaa 420agttgtggtt gtagatggaa cccacttgaa aggcaagtat ggggggtgtt taataacagc 480atccgcccaa gatggaaatt atcagatttt tccactggct tttgccattg ttgatggtga 540gaatgatctg tcatgggaat ggttcttcac tcagctttcc aaattagtac caaaccatga 600agatttggtg tttgtatctg ataggcatgc ttctattttg aaaggaatta gcaaggttag 660taacattatc caatttattt tagaaacatt tttaacgtaa tcggaaaaca aggaagatgt 720aggttatgaa tctttctggt tctcgttgtt acaattattt ttcgtataat atataacgtt 780tgcttaaaac ataatctcag gtatatccct cggccggtca ttgcatttgt attgtccacc 840tgaagagaaa catccgctca aactttaaag gacgacatct cgagtacttg gtggccaaag 900ccgcaagaag cttcagacta gaagattttt acacatcgtt caacgagatt aagagtattg 960atcctagctg tgcggagtat ctgctcgaga ttgggcttga tcattgggct agagcccaat 1020ttcctggaaa acgatataat gtgatgacaa gcaatcttgc ggagtcttgg aatggagttt 1080tgcgtgaggc gagagaacta ccggtcatac gattggtcga gttcattcgt agtaagctga 1140tgacatggtt tgcgagtcgt cgtgaggcgg caaataaaaa taaaggtaac ctatccaaaa 1200aggtgttagg tattgtaacg aaaaactttg acaactctgg tgggtatgag gtattggaga 1260ttgcagagaa ggagtttgag gtccgacaga aagaaggtgg aggtttccat gtcaaccttg 1320ccaatatgac ttgtacgtgc tttgaatttc aaatgctggc aatgccctgt tctcatgcca 1380tagctgcagc cttaaaagga aagatagcgg tagaatcgtt ggtggcacca gtgtatgggg 1440ttactcaact tcaagcagca tacgggggga gtatatatcc tgtcgaagga tacagcggaa 1500ttgcagattt ggtcgccaat gttggtggcg tccaaatctt tcccccaaat acccgacgtc 1560ctccgggaag gccaaaaaag cagagatact tttcaaaggg agaaaaagtg gtaatctaca 1620atacgtatca ttcaaacgtg ttcattcttg gtaactaata tacaacttct gcacttatgt 1680ataacagctt aaacgagggc gtcgcaggac agcttgtagc aggtgcaaaa agttgggaca 1740caacaaagca acctgcaagg aacctatata agaagaagaa ggttggtggg taaggggaat 1800ttagggtcag agggaagatg taagtgagac tggttgtagt atgggaaagc tgtatggaac 1860ggtgaaggat ttaagatggt agaataatga tgtgtgtgta tgtttgtgaa atagtcatgt 1920aaacaaaatc catcaacgga agtggtggcg gtggacgtta tatatttctt ataagaattc 1980ccctctctgt atactgtgat ttctatattt tcagcaataa actaatattg acagtaccaa 2040atatgtagcg aatgaatttc tactaaaatt ctttatggga aaggataacc ggctaacact 2100taaaataaga cttgtaacat aattgcctaa ccagatttgt aagaaatctg gaaaccaatc 2160ccaaatactc tgatcgtcac aaactctaaa ttacgggaaa aggctgtaga gaaggttaca 2220ccgccaactt gatcttagct aataccgtaa agacaacatt acgaaaaatt aatataaatc 2280aaatgttttt gacatatatt ttttgtaacg agctactaga gttaatctct aaactctaaa 2340cctttaaccc gaaaacaata aacccaaaac ctaaacaata taccgtataa ccgttgcagt 2400atcacctata ccctaaacca tagacaatac accctagact gtgatcataa atctgtaggc 2460ggctacatgt ttgcttatgc gtagacaaag atatgttaac ctgctaaatc atgtaacgct 2520aaacaataaa ctctataccg ttaaccatta accctaaaaa cataaagcct ataccataaa 2580ccctaaaccc taaaccagat acggaaaaac ttaaacccta taccctaaac aataaactct 2640ttacggtatt ccataaaccc ttaccctaaa tcatgtaacg ctaaaccata aacccttacg 2700ctgtagccgt ctacattatt tcttatccgt ttaaaaatat atgttaacct gctaaatcat 2760gtaacgctaa accataaact ctataccgtc aaccatttac cctaaaacca taaagcctat 2820accataaacc gttaaccgta aacctaaaac ccataattcg aaaacactaa accctaaaca 2880ataaacccta aaccgaatac cgtaaaccct agccaattta accattaacc ctaaaccctt 2940aacactaaac cctaaaccct aaaacataat aagaaaacac tataccctaa accctaaccc 3000agaattggaa aacactaaac cctaaaccct aaaccatata ccccaaaccc taaacccaaa 3060accttaaccc taaaccatat accccaaacc ctaaacccaa aacctaaacc ctaaacactc 3120aaccataaac actaaaccct aaaccctaaa ccctaaaccc taatccgtaa aacataatac 3180gtaaacccta aaccctaaac ctaacccata atttgaaaac actaaaccct aaacccaaac 3240cctaaaccct aaacccgaac ccacaaagct ctgccttcgt agtaactatg tagccggcta 3300ggacctaaac cgtaaaccta aaacccataa ttcgaaaaca ctaaacccta aaccctaaac 3360ccttacccaa accctaaacc gaataccgta aatcctagcc aatttaacca ttaaccctaa 3420acccttaaca gtaaacccta aaccctaaaa cataattaga aaacactaaa ccctaaacct 3480aacccagaat tggaaaacac taaaccctaa accctaaacc ccaaacccta aaccccaaaa 3540cctaaaccct aaacaataaa ccctaaaccc taaaccctaa accctaatcc atataccctc 3600aaccataatc cgtaaaccct aaaccctatc cactataccc taaaacgttt acaattatac 3660gtaaacccta aaccctatcc agcggaccct aaaacgtata cgagtaacct aaaaagctaa 3720aacctccgcc ggcatagtaa ctctgtagcc gggtccatat taacctatcc gtaaatttac 3780tccgtagccg ggaacatata aactgatccg taaacaaact ctatagccgt catcgtaact 3840atgtagcccg ctacatattt acttaaccgt aagtaaatat atgtaaacca gctaattcat 3900gtaacgctaa accatacacc ctaaacccaa atatctaaac cctaaacact aaacactaaa 3960cgctaaacac taaaccctaa accctaaacc taaaccctaa accctaaacc taaaccctaa 4020accctaaacc ctaaacacta aaccctaaac cctaaaccct aaacactaaa ccctaaacac 4080taaacactaa accctaaacc ctaaacccta aaccctaaac cctaaaccat acaccctaaa 4140cccaaatatc taaaccctaa acactaaaca ctaaacacta aaccctaaac cctaaaccat 4200ataccgtaaa ccgtaaccaa taccctaaac cctaaaactg taaaacctag ccaaattaaa 4260ccaaatctta aagcctaaac cataaaccgc aaaccttaaa ccctatccag tcaacacaaa 4320acataaagca taaacctata acccaaaaac cgccgccgtc atagtaacta tgtagcccgc 4380tacttatttt cttattcgtg aaaatatata tgttaacctg ctaaagcctg taacgctaaa 4440ccggctaatt catgtaacgc aaaaccccaa aacaattact ctttagccgt ctttataatt 4500gcttatccgt aaaaaaaata tgttaacctg gtaaattatg taacagtaat caataaaccc 4560tataccgtta accattaacc ctatacccta taccctaaaa tctaaaccct aaaccctaaa 4620ccctaaaccc tcaaccctaa accctatacc gttaaccatt aaccctatac cgtataccct 4680aaaccctata cgctaaaccc taaactctaa accctaaacc caaaaaccct ataccctaaa 4740cgctaaaccc aaatccctaa atcgtaaacc ctaaacccaa acctaaaccc taaaccatta 4800aaccgtattc cttatccagt taaccgtaat ccttatacct tcgaccatag taactctgta 4860accggctaca tattaactta tccgtaaatt tactccgtag ccggctacat attaactgtt 4920ccgtaaatta actccgtagc cgtcaaagta actctgtagc ccgctacata ttaacttaac 4980cgtaaaccct aaaccctgaa cgctaaaccc taaaccatat accatatacc atataccata 5040taccatatac catataccct aaaccataaa aactaaaccc taaaccataa accctgagtg 5100aaccccataa tagtaactcc atacgcggct atgcattttc atagggggat ttttaaatat 5160gttcacctgc taaccatgac attgttatac ccgactatat aatttttaac cgtatgcatg 5220tatatattaa gctggtaacc atctatttaa caaaattaaa catgtccaaa tattcataca 5280aacatatcga acatccatac tagcctttaa aaggcgtcat aaaagtctta aagggcaaca 5340ataattacga aaattatgca aattttacag cacaacacag cagcccctca tgttcatctg 5400ggattcaatc agcatcttcc cccgttcaat atctttctgc aatccaccaa tttcccctct 5460taagtcagct atatctccct tcaaatgggt caccatctcc tttagatcgt aaatctcctc 5520caccatacat tcatcagtcc aggaaaagag gtgagatttg tcctgcacaa attagccaaa 5580tccggatcgc cttgtttcgg aaagtacttc cattgaaata gaaaaacata aacgatctta 5640agtacctctt cagatccata cggacaacaa tggaaaagcc ttccagggtt ctttgctgtt 5700ccggaggttt taatgacagc ctcttcccca caccgacaaa atgtagggaa ccctctctct 5760cttgcccgcc gcccccgcct cgagcttact ccccctgcaa aaatgtgggt tatggtaatg 5820gatagtcaga acaaagaaac tttaaatcca ggttaattac ataccagacg tagaggacga 5880gcttgccttc cctgattgcg acattcttct tcctctatct ttctcctcct taacgatttt 5940gtaacttaac ctcttccgga tcgtcgcttt gtcggccaat aagagaggaa ccaaatacga 6000ctagaaaaag gggaacattg aatttaggtt acagtattag acgttggccc gaatattatg 6060agcccaagcc caattaatga aaataggtga tattaaataa attacttgac cggctactcg 6120attttgtaac ctccacacag tttccactta attaggtcga tcataacata tgttttcaaa 6180caaacaacaa cactggctga gttaaaacat acgggaatgt gaaaaaaata cattacaaac 6240taggaatgag ttcatatgat gccgtggtat ctctccacaa tggtgacagc aacacgttga 6300agctctgact cgacaacgtt gggggatatg cacttgcaga tttctaatcc gccaaatgcg 6360tgtgcttgta taaggagaac cgcattaaca gccgaataca aatgattctc taattgcggc 6420accagacgtg gtctatcaat cggtaaagcc ttaagatctt tactggaaat ttgtttcccg 6480gcttgtctaa gtaaataagg aaacatggct gcaattgggg caatctcttt gcacatcata 6540acatcagtgc ggatagagat gttgcagtcc agaacatgga gctgccaact ggtggcgtca 6600atgcacaaac caacccaatg tgtcttatcc aagttaaaag ggaagtagaa cctggaagcc 6660gcacctacgc tcctgcaact tgtagaaaaa ctctccaaca aagttgttgg aaatttgaaa 6720ccatccattt tcgccttgtt gaaccttcca taactcttgg ataactgaga tacgaacttg 6780gtgtcgaaga aaactgcgtg atttgttgtt atctgaggag aggcactgtt atataccgaa 6840cgtgtatgat gaataagcag atcaacaacc tagcaataac catacgttca atatagtgga 6900ccctcattag gcacgatata ggtataacaa tattataaac aaataacgga agacagacct 6960tcgccggtac ttgtgcagac ttttccacca gggccattat atccttgctt tcaattttaa 7020ctccgtgaac gatgaacgaa ctaagataaa atgtgtgaac cactaagtta ccataatccg 7080acatttcacg tgtgggaaaa tagaagtttg ttggagatac ttacaacggt gttttaagaa 7140tgtctctcag tttttcgaat ttgtactcag cttccatgtc atcaccggta cgattagccg 7200caacatgtgc ttcccatgct cttgttagaa ttttcttgtc acattcgtag tcacccacta 7260acgcttttgg aacaaccttc tggcgcttgc ttttcctgaa agggtgacaa ggcgcatccc 7320cttcttctgc tgtaagaaca tcatccttgt tcaattgtat cactagagtt tcctcattta 7380cggcatcaca cccttcgatt tgataatttc gctcttcctg actcagtccg agcgagaatg 7440taggtttagg gaacaggagg gcagggtcga gaatctgcgg ataaaacata tgaaataggt

7500tctgatcaga taataaaata tgttaaaatt aacaactcat acggtttcat ctgcggcagc 7560agaaatattg gaatcaggtt cagaaccatt caagttagcc cttccgaggg tgtgcatctc 7620cgtgttaact cccacggtat ctacctggag aacgacaaca aattcacttg aagactatcc 7680gtacggctgt aagtattctg caaaatatcc tcactacccc gctaacattg ttattagcta 7740cctaactttt gactatcaca tatttacttc cgatgtcaac tacataacct tgttaggtgg 7800cattatttac acactgctga aagaaacaca ataaaaaaca tacattggga agttgaaacc 7860cttcctcctg cgcgaaccgc aatgcgctgt cgatgatttt tgaagcatca agttcaccat 7920tagttgaagc tgggggttga ttgaggacct catttaagcc tacatgtgaa taattggact 7980gatcctctcc agtcctccga acctgcggag atgttagggg taaatataaa ttattacttt 8040cacgtacaat tatgcatata tacttacagc tatagacggt cgaacatgtt gtatggccgg 8100agcaggggaa tgattcatct gattacttat actctggaat gtctcagcgt gaaatgactt 8160taacatatct tctactttgg aaacaatggt ggtttcgaga cttgtaaagg cagcaccaaa 8220actcttaaat tctgtggcga ggaaggaaag ttctgcggaa acacggttga gctcttctga 8280tacacgactc agatctcctg aaatgcgacc tttcacagaa ctagctatgg attctgcgtc 8340cacatgatcg ccacaactcg ggtttgattt ctgctttgtt gttttcttct tctctgcttc 8400agttcttgtt tcttcacgca ttctcacaac gtccgatttt gaaacacctc ccgtaaaaaa 8460tgaatggctg aacgaaaatc cctcgtcaat taacttcagc aaattttgta cacccacatc 8520ttcttcatca tcagtaaacc caacctcaga atccccattc acttcctcgt tcccgaccga 8580aataattgaa aacacctccg cctgccgtta catttgttgt cagtcacgat tacacaaaca 8640ttttatacca gttgttattt tcctaccata ttacctttcc tgagcagtca gtctcccggg 8700catgccctgg acttatactt cgcttccctt tcctttcttc ttctgaaagg tcgtcgtcgt 8760ccgcaaagtc gccttcagaa cccgacgacc cggcttcatg aacggcttcg gtaagagcag 8820gtacagcttc aatcattaca agctgtaaag ctagcacaaa tccctttaaa gctattgtgt 8880tttgcgaaag agaaagttcg ttcctctctt tgatactgct tatcagcaac tcgaatgaaa 8940cgcgtcccca aggatatgca agaaacgcat ccaaatcttt aattttctcc gcatgctcgt 9000gtgaaatccg aggtgtatga gtggttggga gaacaacaga agcaaggagt gcaaggaacg 9060catactttat ccttgtttct tttgcagtta cacttctctt tttcagcatc cttacaacac 9120agctgacaga aacttcttcc atggttccga atagctcacc ccagtatggc ttctcgttga 9180tatccctctt tgcctttttt ctggaacgag ctggatactt acaacaattc agacctgtta 9240ctttagcaaa ttccttcaca gaaaaccgga ttggtttgcc agcaaacaag aaccaggctt 9300catgtttctt cttcaccttc aattgcctcg atataatgta tctcccaaaa cgtccagaga 9360atgttggttt ctccgcgatt tctacaagcc taccgaacgg tgatcggcgc agagcttcaa 9420cttcatcttt ttccagtgcc ccgagtatgt tacttattgc gaagggtttg tggtaagggg 9480ttactctcac cccaacgggt tcctcgccca atgcgaacat cctctccggt atcctttgcg 9540aagactccac ttctcgtcgg ttttccatct ccatcgtcgg taccgaaatc ttccgtctca 9600gatgattgcc aaaaaactta aggagagagt ttccagattt gaggcttgtt atacaaaaca 9660gattgattta agagatgagg atttcgattt tcgaatttca aaattacaca agatgctcta 9720taatctttta agatttgagt tttctgacac aagatactca agatacggaa atgatacaga 9780atatcaagga tacgcaaaat ataaaggata cgcaaatctt gaaatatttc actttatacg 9840caaatcttga aatatctcac cctaaagatt acgtaatctt tttctgcctc gatatcgata 9900agggtaggga ggtctttaac atctgctcaa cagtactaaa tagtactaat aggtatatgc 9960ctaaatacgt aatatgtcga gtatagggag cgcaatttct ctatttgttt ttggatgaat 10020gagcgagaga cagaaagcgc aacaacaatc aatacatttt tttaattaaa ttattaataa 10080atgattgggc cttttctctt atatctgagt gcaagctttt tttttgaaag atggcccatt 10140ataaatgggc tatcgtttga attaaaacgt gggccattac tcattttagt agtttactta 10200cagttaaatt ggatagacgt gtgagtgatc agatcccctc tccgattgat tgattctctc 10260ctcctccaaa caagccaagc caaggtatgc cttctctctc tctgcaatga gatcggtttt 10320tcagccggaa gtcaaaacgt atcttcgtat tgttgactac ttcttagatc tattttgctt 10380tttttttcac ttgttatgga ttgctgctag atttaacaca tgatctgctt tcatttaacg 10440gatcaagtta tgattctccg accgaacctc taaaccaaca ctttgaaaca ttaatagtcc 10500tttttagtac tttctgatta atattttacg ccaaatagtg atcagttagt tggatcacct 10560attagcttgc gtcaaaaaaa tgttcagaag ttctattttt gggttgatcc acaaacttca 10620tttatctaac aagacttata gtttgtgtag gagaaaagag agatggaatc gagcagagga 10680ggaggtcaag gtgggatcca gcagttgctt gctgctgaac aggaagctca acacattgtc 10740aatgctgcca gaaccggtaa tttctcttat ttttctactt tcttagtttc ccccacatgc 10800ttgcattctg aatctaaaca taaatagtta aatacagatg atcttgtggc cgaaaattat 10860ccaaacttgt atatttataa atgataagca cttgttgagg ataatgcata gtgtccaatt 10920tgtcttgctg gagctttgat tataaatgtt tggagtttga tttgtgatct tgtgttgcag 10980caaagatggc aaggctgaag caagccaaag aagaggctga gaaggagatt gccgaataca 11040aggctaaaac tgagcaagac tttcagagaa aacttgagga ggtaatatat attctacaaa 11100gtgatataag acaatctagt gttctttttt cttcttcttt gtaataataa tccatgtata 11160tacatacatc tgggggaaaa caaacagaca agtggagact caggtgcgaa tgtgaagagg 11220ctggagcaag agactgatgc caaaatcgaa cagctcaaga acgaagcttc caggatttcc 11280aatgatgttg tggagatgct cctcaaacac gtcaccactg tcaagaactg agtctcacac 11340tcttatttat ttctctgctt tttgtaattt ctctcttgtt gttgtattca tatcttgttt 11400gttgttgttg gatgcgaggc ttgagatgtt atgtaaccaa atacacgaaa caataatgtt 11460ttgagtcttt tgcaagaaat atttcttgat gaataaatct cttttgagaa aacatgtctt 11520tctcctaacg gtttgctact taagtttagt aaagttggat caatcgtgaa atcttttggc 11580aaacagttag tttaagtctg tctgtgtcaa agtcatgggc tcggttcggt tgttcagcct 11640aaaagccata gttaatgagc catagaggtg ttatatattg ggagttatga aaaagcccat 11700attccaccac tatttgctag ggtttctcct cgtcaccgcc ggtgacacct actttaaata 11760tgaatcatct ctctcctcca acttagactt ctctctatct ctctttcagt ttttttggtt 11820catcttcatt atttttccta tctagatgtt ttatttatgt gtgagaaagt acaatgagaa 11880tagatcagat gatgagataa taactgcaac aatatgatct tcttgatgtt cagtattttt 11940ttttttaata caatgttatc ttcgttttca aaatttgtat tggttttgat ttttgaggat 12000aagagaaaga agagatatga tgagcaacta aattggtccg tgtttcagat taaccgtgac 12060cgaaaacttt cttaaccaaa ctctgatctc tagctaacat ctatcttcct tttaaacaat 12120aatttgattt tatttattta tcttgcttgg ttcctgcggt gatgatttta tgaaatattc 12180acatgtcaga cttcagagat tcttgacgaa tcagtgagaa tgtattcata tttctgagca 12240gaagactagt gtttttccaa gcctcataga aagttttcaa ccgttgaaga aattggacaa 12300gaacaaatac gacttaatca ctttaagaac atgattattg ccgcgcagaa agaagaaaca 12360aaacttaata ttgctgagtt gtatcatcgt ggaagttata cattatctaa tacataagtg 12420cttgaagtac gagctaatca ctttgagaat gtgacaattg catttgtttg ccttttatgt 12480taaattataa tgagtcaaca aagatctaca atagtctcca aagatgatca agagcccgtt 12540tgttaagagt ctaagaccgt agcggaacaa ctaaagaata ccctatggac tgttgtggtc 12600gtttatggat ccaaaatgtt gtcctgtctt caaaatatga tggtataatt aggggttcca 12660aatatttctt gaataacaag gcacctccgt gtattacatt ttaacagggt ttaattgtta 12720cgaaactcaa actacattta atttgtttga tatcaagtat tctttctttt tgggaaaatt 12780ggaaaaatgc aaaatgtaat caggtccttc aaaacattat taggtttctt gcttaacaag 12840ctatgtgtta ttcatgatat catgtgataa acaaggtact tcaaacctta cccgaaaagt 12900gtaagtcgaa tgcatcggcc gagtggagcc tataattaag taacacagaa tgtgaacaaa 12960atttactgaa caggttttat aaaacatttc tttaagtcta gttaaacctg ctttcagaca 13020ctttcatatt acaaatctaa aagtgtataa ctaaaagacc aaaataaaat caaactttag 13080taaattttct gtaaatcttc agactgaata tatcagatta gatcatacct aacaaatcag 13140ttcttagcag aattttataa taaacaaatg tagtatgaaa gcctgatatg tcagctactg 13200tgttttgatt ataacctttt taactctttg cacaaatggt ttagaataag aaaaagctcg 13260aaagcaacca aaaagctaaa ttacattttt ctcttctgca tattctggaa tccttggatt 13320ttattctttc acagttttta ttttttattt tcttttcttg accaaaaatc acaccagaac 13380gctaataacg acagatagat ttttatgtaa acattgataa gtgacacagt ggaccatgtg 13440atacgttagt ttgttagtaa tcacatttaa taaaaagttt cccatcacta tcagctcacg 13500gacgatatta cgctatagcg tcaagtcgtg gctccatttt gactagaaat taaaaaacaa 13560taaagcatat ttttatttat tttaggtatt agtcttaacg gcacggccag tgacgattga 13620ttgtcccttt tatgtcctcg tgcttaatca gcgttcgtta gatattctta atatatttac 13680tttccaaagt ctatctatgt tttgccttct tcacaagcat tatatattgt cgtcgctatt 13740gctctgcctt gtgatcatca ttgttttact ggtaactctc tctttgtctc cttcatcttg 13800ttctctgcga gagaggagta gatatggaag tagaagaagg agcgagtgtt gatgttggtg 13860gtgggaagat aaaggttgga gtatgcgtca tggaaaagaa ggtgaaatgc ggctccgatg 13920ttatctttca tttttttaaa aaaaaattgt tactgctcga tgttttcttc tactgaccaa 13980cagactcgga tggttggttt gtagtttatt ttggagtcct cttgttttta ctatgtgttc 14040ttctcgatcg ctcaaagata gctcgttgtg gatttatagg tctcctcaag tcccatgggt 14100caaattttgg acagactgga gtcgtttggt gaatttgagg taactttgta ctcacgggtt 14160tcttctttgt tccaagattt ttatatgcta taattttctg gtttgtagta ctctatatat 14220agtttactat ttaacctgga gagaggttgc ttttgattag atggcttgtt atttagcttg 14280ggagatgctc tttttgtgtt cattttccaa gttgttgaat tagtttgcga ctagaaatgt 14340tgttgactaa tttgtctttg tatattctct ggtgtattct ttatattttt catgtcatgg 14400acaactttat ccgcagatct tacattttcg ggataaagtc atacttgaag atccaataga 14460aaggtttgtt gtactaaaga gtttactttt tcccttttcc agttatgaat gacgtcccat 14520tattagtgta ctaggagaaa tagtagttaa tctctatata tatcaagtac tttctccaat 14580atatatatat atatatatat atatatatat atatatatat atatcaagta caaaaacctg 14640acatgagaat ttgctgtttc agctggccca tttgtgattg cttgattgct ttccattcca 14700ctggatatcc tcttgaaaag gctcaggctt atgctgcttt aagaaagtga gtttttttca 14760ccttttttat ttcttgattt tggctcccat ataaaagctt tctcatgact ccggcttctg 14820aggttgacaa cttttcaaac ttgtcttgtt caggccattt ttagtgaatg aactagatcc 14880acagtatctt cttcatgatc gccgcaaggt gtacgaggta ttgcatatta ggatgcaata 14940agttggttct gtgtactttt gataattttt caaagtttcc agggctaatg agacaaagaa 15000gagctactac atatatttta tttttccttg ctactaacaa atagcttttg tgtgcatttt 15060tcagcatctt gagatgtatg gaatcccagt tcctaggtat gcttgtgtca acagaaaggt 15120accaaatgaa gaagtagatt attttgtcga ggaagaagat tttgttgagg ttaatggtga 15180acgcttctgg aagccatttg tggaaaagcc tgtcaatggt gatcttctct tttttgcctc 15240ttgctctctc tttttcatgc cagcttatta tctatttatc cataagtcta aagtagaaaa 15300ataaccatag gagtgtgttt tgtattttcc tctgatattt cgtaaccaat ttcacttgaa 15360tctgttatct catatgagtt aaatattgag gtacaatgaa tataaacaga ggtgattttt 15420caaggctata ttctcattta atgttcttag ttgttatatg atgatggcta tcctcgttat 15480atttatcttc cgacagttaa cattctttgt gcgtttgtag gagatgacca tagtatcatg 15540atatactacc ctagctcggc aggtggaggc atgaaagaat tgttccgcaa ggtatctctc 15600cagaagaatt tggctgctat ggattcaatt gttgatattc taatatattt ttcataataa 15660ttagtgtcat attcctctgg gactcgtttt cagctaatgt atctcgttcg tctccatttt 15720actttaagat tgggaaccga tcaagtgagt ttcatccaga cgtcagaaga gtaaggagag 15780agggttcctt tatatatgaa gagtttatgt ccaccggagg aactgatgtc aaggtatgtg 15840tttattttcc ttctgcatgt ttctctatta gctcatttca tcttttagac cacattaatc 15900tttctgagaa tatcgtacta ttcttggtgt caagtgtcct acatttataa ggttatcatg 15960ttaatggcct atatgtttat atgacttagc tcctatttgc tggttgtttt tttgtctggt 16020gaacattttt tcgtgaagtt ttggctttca cgagctctgt actgacaatc tggatgctgg 16080cttaaataca ggtatacaca gtgggtcctg aatacgcaca tgctgaagca agaaagtcgc 16140ctgttgttga tggtgttgtt atgaggaata ctgatggcaa ggaagtacgt cgtctaccga 16200tcatttacct taaatagaat gaaatttgac actatctcat tttcttatgg agagagggct 16260atattttagg tgcacaaaaa aaaagaagag gggctatatt ttgaatagaa tatcaaagtc 16320tattgattat gatttggtaa atgtttcatg atggtgtaac actattattt tattttttta 16380ttgaacaggt gagatatcca gtgttgctta cccctgcaga aaagcaaatg gctagacaac 16440tttgcattgc atttaggcaa actgtatgtt gtgaatccaa tccctctggc tctcgcagag 16500ttttattttc tttataatgg tactaaatta tttttatttt ttttacaaat ttcatcaggt 16560gtgtgggttt gatcttctac gatctgaggg atgttcatat gtttgtgatg taaatggatg 16620gagttttgtc aagaactctt acaagtaagc taatagcatt cttcacttta ttttaactct 16680ctaaaatgtg caccgttaaa gtattattca caaacctctc atactatact atgtaaggta 16740ttacgacgat gctgcttgtg tgctaagaaa aatgtgtttg gacgtaaagg ctcctcatct 16800gtcatcgact ctccctcaca ctttgccttg gaagttcagt aagcctgtac agcctaataa 16860aagactaacc cgtcaaggga atggacagtc agaagagttg cgttgtgtca ttgctgttat 16920tcgacagtat gtttttttta gcatataaat cacctatatc tcatctatac ttctctctgt 16980taaaattttg ttattacatg acagtggtga tcgaactcca aaacagaagg tgaaactaaa 17040tgttacagag gcgaaactgt taaacctgat gttgaagtac aatggtggaa agccaagagc 17100tgaggtgagg agatcgactt tttctgtcat ccatctttta tctgccttgt tcattttata 17160tattttgtca aattttgcag acaaaactaa aaagtgcagt ccagttgcaa gacctattag 17220atgccacaag aatgttagtt ccccgtacaa ggtacaaccc tgaactctat cttctctcca 17280aaaagcatgt ctggtctacc ttttccatgt gttgaatact cgtaccgaca atatggtctt 17340tcttttttta tatgtagacc aggtcgtgag agtgatagtg atccagaaga ccttgaacat 17400gctgagaagc ttcgccaagt taaagcagtt cttgaagagg ttctttagta ctttctcttt 17460tttttttttg aatatagtcc gtggaaagta ttacatagta tcatttaggg agttaaaact 17520cttataagta agctagcatt atgcattttt tatttaattc atgaggtcat attcttatag 17580cagaaaacct tttggtatgg cgcagggagg aaatttttct gggatataca ggaaggttca 17640gctgaagccg ctgaagtggg atggtgaagg cgaagaagaa agacctgtag aggcccttat 17700gattcttaaa tacggcggtg ttctaactca cgctggtaga aaacaggtgc ttacatactc 17760taccatttaa tttgtcattg ctatatatat atatatatgt ttcccaaata tggaaaattg 17820tttaacttat tgatgtttgc ttcatctgca tgcaggcaga agagcttggt agattcttcc 17880gatacaatat gtatccaggt aaggccgcct aatctgtttt tttttatttg ttactttttt 17940tttggagtat ttgacgatga aatggcttta ctatatgttt tttcaggtga agggactggt 18000ttgcttcgtc tccatagtac ataccgtcat gaccttaaaa tttacagctc tgacgaggga 18060cgtgttcagg tacgtacacc ttttacattg atactactca agataattaa acatatgtgt 18120tggaaaaaat aattattgaa ttggtttgca tatgttgacc agatgtctgc agctgctttt 18180gctaaaggtc tgcttgacct agaaggacag ctgacaccaa tcctggtttt tctttctttt 18240tttaaatctt gttggtgctt aatttgcaat gtcatcatgt gtattactaa taatattttt 18300atctgtcttg cttctctagg tttctttggt tagcaaggac tcttccatgt tggatggtct 18360tgatactgcc agcattgaaa tggaagcggc caaggtttgt cccatgatga atcattttct 18420gaatagtcca ccttaaatat gttaacagtt ttgatttcac tacttggact atgcaggcta 18480gattgaatga gattgtaacg tgtggcaaaa aaatgaccaa tgagcatggc tcctctgaag 18540aattcccttg gatgactgat ggagctggag ttcctcccaa tgctaatgga ctcctccgtg 18600aattggtact cacacttttc catctagtct tagtttacta ctggtgtgaa gtacatcacc 18660aacctttttt ttttagagtt ttgtttgcac aacaacgtac tattgcatag gcactgatga 18720gcacattggt aacctctgtg ggtattgctc tcatttttgt ggctccctca ttaggtgaca 18780ttgaccaaga atgtgactga acaagtaaga atacttgcaa tggatgagaa ggagaacctc 18840actgagccat atgatatgta tgatcaagca aaggctcttg gaaagacaaa cattgacagt 18900gataggattg cttctggatt accatgcggt agtgaagggt ttcttctcat gtttgctcgg 18960tggataaaac tcgcaaggga tctctataat gaaagaaaag agtaagtttc ttatcaaatt 19020ttttaaacat tatttagagg cttgtgagtg agtgagagta gcactaatag ttcagtttta 19080acttgcagcc gatttgacat cacgcagatt ccggatattt atgattcatg caagtaagtt 19140atatatgtta tggtggtttt ttggtgtatc tcgtttatga atttacttta atatgtctgt 19200taacgtcacc aggtacgacc tgttgcataa ttcccatcta ggtctacaag gattagatga 19260actcttcaaa gttgcacagg tacaaactgt attttctctt ttccagtttt cacttacaat 19320aatactttgt tatcttccaa ggcatactaa tgaacatgac ctttcttggt agatctctgt 19380attgcgttga ctgtgttagc aattatgact aaataatgtg ttttgtggtt gcagttgctt 19440gcagatggtg tgatcccaaa cgagtatgga atcaatccac aacagaagct taaaatcggt 19500tcaaaggttg acattttata ggctagctac gagagtagtt tatatatggt gtttccagtg 19560gatctctcac atctttatca aaatctattt ttccgtgaaa aaaggttgct aggcgcttaa 19620tggggaaaat cttgatagac ttgaggaata ctcgagaaga agcaatgagt gttgctgaac 19680tcaaagaaca acatgtcaca ttgtcattat ctgcctcgaa aaaacaacaa gacaaaaata 19740ttcaaccaaa gagagatgat ttgagacgac ctggcactgg tgataaggat gaagatgatg 19800acgataaaga aaccaaatac cgattagatc ctaagtaata gcttactctc tgcttgtata 19860gtttctttct tcttctcctt ttcttggttt ttgcatattc ttaagtgttt tatactaatt 19920gcttttcatt ttcccatttt aggtatgcaa atgtcaagac acctgaacgt catgtgagga 19980ctcgactcta cttcacatct g 2000113141DNAArtificial Sequencesynthesized oligonucleotide 131gaaggtcgga gtcaacggat ttgaaacggc aggtgaggct a 4113223DNAArtificial Sequencesynthesized oligonucleotide 132yccaagctga gtgtgaggct gaa 2313343DNAArtificial Sequencesynthesized oligonucleotide 133gaaggtgacc aagttcatgc tgggaggtct ttaacatctg ctc 4313424DNAArtificial Sequencesynthesized oligonucleotide 134atgtattgat tgttgttgcg cttt 2413520DNAArtificial Sequencesynthesized oligonucleotide 135tctctcgctc attcatccaa 2013623DNAArtificial Sequencesynthesized oligonucleotide 136tgattcgtat tgaaagcaac aaa 2313717DNAArtificial Sequencesynthesized oligonucleotide 137ctctaaccca aagtaaa 1713821DNAArtificial Sequencesynthesized oligonucleotide 138tcgataaggg tagggaggtc t 2113919DNAArtificial Sequencesynthesized oligonucleotide 139ggcatatacc tattagtac 191404756DNABrassica napus 140ttttaatata caacgtgcca aacttcgtcc atttataatc ttatatattt ttgtcttcgc 60atatgataga tcataagatt tggagaaaca tataaaatta acctatgaaa ttaaggggat 120ctatatatta tagtatgtat ccaaagaaca tatatatgaa atatttgaat acatcataag 180atttggggaa tataaattaa tataaaataa aatcgtccaa ttttaaacgt aaacgtctcc 240gctgtattta tataaacttt tcaaaatcac ggtgtcgctc gtgttaagat ttggatttct 300aatccaactt tcagaaagtt tatgtgaaag ataatactca aacacaatag ttcgtttaga 360acgctcttaa gattaatgtc acacacacaa aattgatgtc actagattta tatgttttgt 420cgtttaaggg gatccatcaa ctacacatgg aagaatagaa aacactggtc acacacaaaa 480aataatataa gcatgttgag tcctattcat cagaaaattt tctccaaagg atgaacaata 540acctacaatt aaggaaactt attataacaa cgaccaagaa gttttcccat aagtcatgct 600ttcgtccata ccttcgggtc tttcaagcaa gcttcacaca tgccttatcg tatatttgta 660tacgttttca ttttgtgtac gtctattaat tagtatatgc ctagattata tatttatata 720gtcgacaatt acctatccta aaggtttatt ttaattttta ttcagaaaga tatgcacgta 780gggccactag tgtttcccgt tgttattgga aattccatgt gttgaactgt ttgattaagt 840ttttgaaccc gtaagtcaaa tttatttagg tttaacaggg tttcattact gttataagct 900tgtcaagaaa gaatttgtca caaatttcga gactcatgca tgcatgcatg tttcttaact 960ttcgagaaac atgaagtgtg gttttaccag ctgaagtttg tgttaaaaca catttttaat 1020ttgaccacgt gtgatattca aatatcaaca attctgttca acgggagatt catacatatg 1080ttcagatgtt ctttaatctt gagcacctaa ctttgcttcc catacgcaaa tgtgtgtgcc 1140gcgaactatg gtgaatgtac ggcatcgttt ttctttttgg agcattttta aaagaaaata 1200taatttagtt atttccgttt tgattgatat agatgcatta ataaaccgac ttacgtgggt 1260gatgggatga gcaaaattaa aatatgcatt ttagtaacca attacggtcc atcttgacaa 1320actaacaaac aaaaagtcta ttatgacgtt gatttgtgac cttgccatat aaatgccaaa 1380atttcattct ttcaataagc aacgagatca ataagatgtg

gcagtcacgt gaacgttagt 1440tatcgccatt tcaactgaac cttcacgaac atataaagtt catttgttct ttgctaaaat 1500tacgcaaatc tctgttgtgc acgaaattac gactattttt taatgacaac tcctgttctt 1560gtatcgtgta tctagcacgt aaatttgaat tatggcggca ttatcgataa aatatgtaaa 1620gaatatattt tcatcataca ccacaaacaa atgttctgga aatatcacct tttatcacat 1680atacaaagct gaccctgaat ttttggaaac tacaagccaa aaaaaaatgg acaaaagaaa 1740aattatgcac aaaagtggac tcgtcctaac tctttttata atgataatta acctttgaac 1800aactaattaa tacatacaat atgttttttt tttctaaaaa ttggtcgatt aaatattcat 1860atataatcct catcatcatt tattagcctt ttgtagctta cttaaaaaat cacttcccac 1920caaattgatt attttttcac ttcatacgtt gatcaattta aaaaaaaaat aataataata 1980tatgcacgtc cggcgtccca tgacttcaga cgcttttctg tttccttgat tttataggtt 2040agaaatttcc aaaatgctca tatttttcac tgatatgtat tatacgcaca aaatagtaaa 2100gtcataaaaa tttatgatgg ttcttttata tgtatagagt agtgaaaatt tatatatatt 2160gtagttccgc ttaaaataaa ttaatatttt tcttttatcg cccatattta agttactaaa 2220cgtatataat taaaaactga aatgcaatat gtagaaatcc gggcattgat tgttgattaa 2280cattgatata cccatgcgac tggcttatga tgaaccatac ctgctaatac tcattgttac 2340aaactcgcag caacatcata aatccatcac tcgccacaat aattttcttc agtgtatgtt 2400caattgatca cattttgacc cttcactttc ttgaatgaga aattatacaa gaaagtgatt 2460tgagtgtgtt aaaaaatgat ttgtcattct gaccaattag ctagggtttc cactccctca 2520cgctaacaca catcaccgca gccatttcca tctctcaacc aacttcaaac aatggctgaa 2580cgaggcggag aacgaggtgc cgagcgagga ggagaccgcg gcggtttcgg acgcggattc 2640ggaggaggtc gcggcgacag aggaggccgg ggaggtccga gaggccgagg cggcagacgc 2700ggaggacgtc cgacagagga ggagaagtgg accccagtga ccaagctcgg ccgtctcgtg 2760caagccggaa agatccagaa gctcgagcag atctacctcc actccctccc cgtcaaggag 2820taccagatca tcgacctcct cgtcggcccc accttgaaag acgaggtgat gaagatcatg 2880ccggttcaga aacagaccag ggccggtcag aggacgaggt tcaaggcctt tgtcgtcgtc 2940ggagacacga acgggcacgt tggtttgggt gtgaagtgct ccaaggaggt ggcgacggcg 3000atcagaggtg ggattatatt ggcgaagctg tcggttattc ccgtgaggag agggtactgg 3060gggaacaaga ttgggaagcc gcatacggtt ccttgtaagg ttacggggaa gtgtgggtct 3120gttacggtga ggatggtgcc tgctccgagg ggtgcgggga ttgtggcggc tagggttcct 3180aagaaggtgc ttcagttcgc tgggattgat gatgtgttta cttcgtcgag ggggtctact 3240aagactcttg gaaacttcgt aaaggtttga actttcttca tttatttaga gtttggagtt 3300gtgtatggat tgaaagttga ttcctttttg atggttgcgt gtggagttgt tttgggtaat 3360ttagcttgtt gtagttgatg taaagtgaga attgctaagc ttgattagtt tgaataagct 3420gtctgtgtta aatgatgatt tgtttgtgtt gtattgagct gtctgaattt cattatttgt 3480ttgtgttatt gctaaattac aagcttaaat ttgataactt ttgattcttt cgtgagagtt 3540ttttttgggt aatatagctt gatgtagttg atggaagtaa attgttaacc tttagagctg 3600tctgttaatg gtgatttgtt tgtgttgtat tgagctgtct gaattataat ttgtttgtgt 3660ttattgctaa atcacaaagc tttgaagtcg tttgtacata ttcatcattt ttgtgtttat 3720tgacttgttt gtgtttattg ctaaatcaca agtttctatg attaatcttc atgatttggt 3780tgtgtttatt actaaatcac aagtttctat gattaatctt aatgatttgg ttgtgtttat 3840tgctaaattt gagattacat tataatctta gtttatacat gtataacatc atttcacttg 3900aattttcaca gacgagctgg tgatatgttt ttttagtatt ttccatgtat tgctttgtaa 3960gaacttttaa atgtttgttt tcttcctttg aagaacaatt aaatgtttgt tcagtatttt 4020tgaatcattc attggttaat catgtgtaat tttatttttg taggctacat tcgactgtct 4080ccagaagact tatggattcc tgacaccaga gttctggaaa gagacgagct ttaaaaagtc 4140accgtaccag gagtacactg atctgctggc cgagaaggga attgcaacca aagccatcac 4200cgaggttgaa gaccaagccg cctcctaaat aaacaaacaa acaatgtccc ccttgttttg 4260tttttggatg atcctttctt gagttttatt ttgttcgaac ctttagtata tgttgtttgc 4320tggattgctg ctttcttatg ttttcatcat ctcccccaaa caaagttatt tttgctctag 4380ttttttttta taatcgatgg ttatgccctc tagtattttt tttacttgaa tttttcacta 4440attttctatc aacttcccta tgttacactt tctaaggttg attacatagg tggattgttg 4500tgaaacaaat actaataata aactaatact tttagaataa tataattagt gggattaaat 4560tataaattat aaattaaaaa gacaagtctg aaatggattg tttaactgta aaaggtaaag 4620agactttgaa tagaagagaa taaatcaaaa gttgacgtat cttctccgaa atttcagcta 4680tttctaaata cgcggattct gattactctg ttctctctcg cctgataaac cttagaattg 4740agtttttttt tttttt 47561416519DNABrassica napus 141ttagttccaa tcaacaacga ttgaaacaga gcgagcaaag agaagaggat atcataagcc 60accaactaca ccaacggaac caagtgctga caaaccaaca ccgaaggaga aggtgtggtc 120tccggagtca aaagccgttc gcccaccaag agaaagatgc ggcaccggaa ctggggccgg 180acatgcaacg ccgacagaac cgactatgtc ggagaagaaa cccgaaagcc acagactaag 240gatcgccata aagatcccgg cgattttgat cttaaacaaa aagataaacc gagaatcaaa 300acagtaaaaa cacaaacctt cccgaccgac ggtggtaaag agaaaccaca ccgccggtcg 360gagaacccga tttcaagttt tagttttttc tagaaagagg tcagaggatc tagagagatg 420gtgatgggcc aaaaaactta tttacgttta agcctgtaat atttgataaa ttcaaccgag 480taaaagaaac acgaggtaat tttttaatgt aatatgaatc atatgatata tgtcaccagt 540atgtcgtcac attgatctca ccaacccaca ttcgtatatc cacaacatgt cgcgtgaatg 600cgatttacat ataaaataaa aaggagtcga ctgtgaccgg taactttgtg gccattccat 660aatttccata ccaactaatt taccagaaac tattttataa gtattaaaaa cgacggtcca 720gtggttttga gggagcttcg gccccggacc agggttctaa tcccgctggc cactggggca 780aaatataaca tttgggtttc cccagagtgt tcggtgacca gggcgtacta cagccgataa 840tgtaagctgc tatgaacact cgcccctagt ctagacccat ttcggtggag gccatgatac 900acaagattat ttaaaaaaaa aggatatgtt gcagaaaaaa aagtataaaa aagatatagt 960tgtacacaac caggggcgga cgcactaaca acggtacgag gtcacgtgcc ccctactgat 1020ttttattttt cttaaataac ataaattaat ttgatgaatt attgttagtt tactggagaa 1080aattgaaaat gtgcccccat caaaacttca tttttactgt ttctattgtg cccccgatta 1140gaaacgtttc tagatcctcc ctgtacacaa cacccgttcc ttgactttta tcgtccttta 1200ccgtttatac atccatatat ttatttttac caaaagattc atgtcattat ggttcgtgca 1260agtttctggc aattcgaaat agcatcattt gcagcatcat gcatgatgca tatactcaag 1320tttcatgata ttcctaataa aattgggttt ctgttgattt cagaatccat actattcata 1380attaggtgtt tccatacata ttatacatgt gggtatacgt acacgcacat cagttaattt 1440aactataact ttggactagt aactgtattt gaatatggct ttctcttttc agaatattct 1500acaaaacagt ttcaattccg tttgtcagtg gaatcccata tatggatgga catagttgga 1560catatttagg tccttatcca aatgatgttt taagatttga tttatggatg cacatagttg 1620gatacatttt atgtttaaaa tgtgttttta atattattat tgctgtggtg tgaaattttg 1680tgtattcact ttagttataa tatctatgta taccaaagag aagaagataa catatattat 1740agtattggta catactataa tataacatac ttaattagta aatttcacac cacagcaaaa 1800ataatataaa aggtcacttt ttaatataca acgtgccaaa cttcgtccat ttataatctt 1860atatattttt gtcttcgcat atgatagatc ataagatttg gagaaacata taaaattaac 1920ctatgaaatt aaggggatct atatattata gtatgtatcc aaagaacata tatatgaaat 1980atttgaatac atcataagat ttggggaata taaattaata taaaataaaa tcgtccaatt 2040ttaaacgtaa acgtctccgc tgtatttata taaacttttc aaaatcacgg tgtcgctcgt 2100gttaagattt ggatttctaa tccaactttc agaaagttta tgtgaaagat aatactcaaa 2160cacaatagtt cgtttagaac gctcttaaga ttaatgtcac acacacaaaa ttgatgtcac 2220tagatttata tgttttgtcg tttaagggga tccatcaact acacatggaa gaatagaaaa 2280cactggtcac acacaaaaaa taatataagc atgttgagtc ctattcatca gaaaattttc 2340tccaaaggat gaacaataac ctacaattaa ggaaacttat tataacaacg accaagaagt 2400tttcccataa gtcatgcttt cgtccatacc ttcgggtctt tcaagcaagc ttcacacatg 2460ccttatcgta tatttgtata cgttttcatt ttgtgtacgt ctattaatta gtatatgcct 2520agattatata tttatatagt cgacaattac ctatcctaaa ggtttatttt aatttttatt 2580cagaaagata tgcacgtagg gccactagtg tttcccgttg ttattggaaa ttccatgtgt 2640tgaactgttt gattaagttt ttgaacccgt aagtcaaatt tatttaggtt taacagggtt 2700tcattactgt tataagcttg tcaagaaaga atttgtcaca aatttcgaga ctcatgcatg 2760catgcatgtt tcttaacttt cgagaaacat gaagtgtggt tttaccagct gaagtttgtg 2820ttaaaacaca tttttaattt gaccacgtgt gatattcaaa tatcaacaat tctgttcaac 2880gggagattca tacatatgtt cagatgttct ttaatcttga gcacctaact ttgcttccca 2940tacgcaaatg tgtgtgccgc gaactatggt gaatgtacgg catcgttttt ctttttggag 3000catttttaaa agaaaatata atttagttat ttccgttttg attgatatag atgcattaat 3060aaaccgactt acgtgggtga tgggatgagc aaaattaaaa tatgcatttt agtaaccaat 3120tacggtccat cttgacaaac taacaaacaa aaagtctatt atgacgttga tttgtgacct 3180tgccatataa atgccaaaat ttcattcttt caataagcaa cgagatcaat aagatgtggc 3240agtcacgtga acgttagtta tcgccatttc aactgaacct tcacgaacat ataaagttca 3300tttgttcttt gctaaaatta cgcaaatctc tgttgtgcac gaaattacga ctatttttta 3360atgacaactc ctgttcttgt atcgtgtatc tagcacgtaa atttgaatta tggcggcatt 3420atcgataaaa tatgtaaaga atatattttc atcatacacc acaaacaaat gttctggaaa 3480tatcaccttt tatcacatat acaaagctga ccctgaattt ttggaaacta caagccaaaa 3540aaaaatggac aaaagaaaaa ttatgcacaa aagtggactc gtcctaactc tttttataat 3600gataattaac ctttgaacaa ctaattaata catacaatat gttttttttt tctaaaaatt 3660ggtcgattaa atattcatat ataatcctca tcatcattta ttagcctttt gtagcttact 3720taaaaaatca cttcccacca aattgattat tttttcactt catacgttga tcaatttaaa 3780aaaaaaataa taataatata tgcacgtccg gcgtcccatg acttcagacg cttttctgtt 3840tccttgattt tataggttag aaatttccaa aatgctcata tttttcactg atatgtatta 3900tacgcacaaa atagtaaagt cataaaaatt tatgatggtt cttttatatg tatagagtag 3960tgaaaattta tatatattgt agttccgctt aaaataaatt aatatttttc ttttatcgcc 4020catatttaag ttactaaacg tatataatta aaaactgaaa tgcaatatgt agaaatccgg 4080gcattgattg ttgattaaca ttgatatacc catgcgactg gcttatgatg aaccatacct 4140gctaatactc attgttacaa actcgcagca acatcataaa tccatcactc gccacaataa 4200ttttcttcag tgtatgttca attgatcaca ttttgaccct tcactttctt gaatgagaaa 4260ttatacaaga aagtgatttg agtgtgttaa aaaatgattt gtcattctga ccaatccctc 4320acgctaacac acatcaccgc agccatttcc atctctcaac caacttcaaa caatggctga 4380acgaggcgga gaacgaggtg ccgagcgagg aggagaccgc ggcggtttcg gacgcggatt 4440cggaggaggt cgcggcgaca gaggaggccg gggaggtccg agaggccgag gcggcagacg 4500cggaggacgt ccgacagagg aggagaagtg gaccccagtg accaagctcg gccgtctcgt 4560gcaagccgga aagatccaga agctcgagca gatctacctc cactccctcc ccgtcaagga 4620gtaccagatc atcgacctcc tcgtcggccc caccttgaaa gacgaggtga tgaagatcat 4680gccggttcag aaacagacca gggccggtca gaggacgagg ttcaaggcct ttgtcgtcgt 4740cggagacacg aacgggcacg ttggtttggg tgtgaagtgc tccaaggagg tggcgacggc 4800gatcagaggt gggattatat tggcgaagct gtcggttatt cccgtgagga gagggtactg 4860ggggaacaag attgggaagc cgcatacggt tccttgtaag gttacgggga agtgtgggtc 4920tgttacggtg aggatggtgc ctgctccgag gggtgcgggg attgtggcgg ctagggttcc 4980taagaaggtg cttcagttcg ctgggattga tgatgtgttt acttcgtcga gggggtctac 5040taagactctt ggaaacttcg taaaggtttg aactttcttc atttatttag agtttggagt 5100tgtgtatgga ttgaaagttg attccttttt gatggttgcg tgtggagttg ttttgggtaa 5160tttagcttgt tgtagttgat gtaaagtgag aattgctaag cttgattagt ttgaataagc 5220tgtctgtgtt aaatgatgat ttgtttgtgt tgtattgagc tgtctgaatt tcattatttg 5280tttgtgttat tgctaaatta caagcttaaa tttgataact tttgattctt tcgtgagagt 5340tttttttggg taatatagct tgatgtagtt gatggaagta aattgttaac ctttagagct 5400gtctgttaat ggtgatttgt ttgtgttgta ttgagctgtc tgaattataa tttgtttgtg 5460tttattgcta aatcacaaag ctttgaagtc gtttgtacat attcatcatt tttgtgttta 5520ttgacttgtt tgtgtttatt gctaaatcac aagtttctat gattaatctt catgatttgg 5580ttgtgtttat tactaaatca caagtttcta tgattaatct taatgatttg gttgtgttta 5640ttgctaaatt tgagattaca ttataatctt agtttataca tgtataacat catttcactt 5700gaattttcac agacgagctg gtgatatgtt tttttagtat tttccatgta ttgctttgta 5760agaactttta aatgtttgtt ttcttccttt gaagaacaat taaatgtttg ttcagtattt 5820ttgaatcatt cattggttaa tcatgtgtaa ttttattttt gtaggctaca ttcgactgtc 5880tccagaagac ttatggattc ctgacaccag agttctggaa agagacgagc tttaaaaagt 5940caccgtacca ggagtacact gatctgctgg ccgagaaggg aattgcaacc aaagccatca 6000ccgaggttga agaccaagcc gcctcctaaa taaacaaaca aacaatgtcc cccttgtttt 6060gtttttggat gatcctttct tgagttttat tttgttcgaa cctttagtat atgttgtttg 6120ctggattgct gctttcttat gttttcatca tctcccccaa acaaagttat ttttgctcta 6180gttttttttt ataatcgatg gttatgccct ctagtatttt ttttacttga atttttcact 6240aattttctat caacttccct atgttacact ttctaaggtt gattacatag gtggattgtt 6300gtgaaacaaa tactaataat aaactaatac ttttagaata atataattag tgggattaaa 6360ttataaatta taaattaaaa agacaagtct gaaatggatt gtttaactgt aaaaggtaaa 6420gagactttga atagaagaga ataaatcaaa agttgacgta tcttctccga aatttcagct 6480atttctaaat acgcggattc tgattactct gttctctct 651914223DNAArtificial Sequencesynthesized oligonucleotide 142ttgtcaaaaa gaggagagag agg 2314320DNAArtificial Sequencesynthesized oligonucleotide 143gaaagatcaa gaacacagga 2014421DNAArtificial Sequencesynthesized oligonucleotide 144cgaccaaaaa tatgaaatac t 2114521DNAArtificial Sequencesynthesized oligonucleotide 145gctacggcta acacacatac g 21

Claims

1. A method for introducing a genomic modification that contributes to pod shatter tolerance in B. napus plant, the method comprising introducing a targeted modification into the genome of a B. napus plant, plant cell or seed thereof wherein the targeted modification includes excising endogenous genomic sequence of an INDEHISCENT (BnIND), ALCATRAZ (BnALC), or POLYGALACTURONASE (BnPGAZ) gene in a B. napus plant cell.

2. The method of claim 1, wherein the targeted genomic modification comprises first and second double strand breaks induced by a TALE-nuclease (TALEN), a meganuclease, a zinc finger nuclease, or a CRISPR-associated nuclease.

3. The method of claim 1, wherein the method comprises introducing a CRISPR-associated nuclease and guide RNAs into a B. napus plant cell.

4. The method of claim 3, wherein the guide RNAs comprise: a. a first guide RNA comprising SEQ ID NO:26 and a second guide RNA comprising SEQ ID NO:27 that catalyze targeted deletion of endogenous genomic BnIND sequence in the plant cell; b. a first guide RNA comprising SEQ ID NO:28 and a second guide RNA comprising SEQ ID NO:29 that catalyze targeted deletion of endogenous genomic BnPGAZ sequence in the plant cell; c. a first guide RNA comprising SEQ ID NO:30 and a second guide RNA comprising SEQ ID NO:29 that catalyze targeted deletion of endogenous genomic BnPGAZ sequence in the plant cell; d. a first guide RNA comprising SEQ ID NO:31 and a second guide RNA comprising SEQ ID NO:32 that catalyze targeted deletion of endogenous genomic BnALC sequence in the plant cell; e. a first guide RNA comprising SEQ ID NO:33 and a second guide RNA comprising SEQ ID NO:34 that catalyze targeted deletion of endogenous genomic BnALC sequence in the plant cell; or f. a first guide RNA comprising SEQ ID NO:35 and a second guide RNA comprising SEQ ID NO:33 that catalyze targeted deletion of endogenous genomic BnALC sequence in the plant cell.

5. The method of claim 1, wherein the excised endogenous genomic sequence comprises: a. Nucleotides at position 7740 to position 10346 of SEQ ID NO:56; b. Nucleotides at position 2676 to position 5101 of SEQ ID NO:57; c. Nucleotides at position 2720 to position 5866 of SEQ ID NO:60; d. Nucleotides at position 2720 to position 5865 of SEQ ID NO:60; e. Nucleotides at position 2705 to position 5804 of SEQ ID NO:61; f. Nucleotides at position 2704 to position 5802 of SEQ ID NO:61; g. Nucleotides at position 2705 to position 5802 of SEQ ID NO:61; h. Nucleotides at position 2019 to position 4441 of SEQ ID NO:67; i. Nucleotides at position 2018 to position 4639 of SEQ ID NO:69; j. Nucleotides at position 2018 to position 4446 of SEQ ID NO:70; k. Nucleotides at position 1723 to position 2849 of SEQ ID NO:71;

l. Nucleotides at position 1722 to position 2851 of SEQ ID NO:73; m. Nucleotides at position 6369 to position 7511 of SEQ ID NO:72; n. Nucleotides at position 6368 to position 7510 of SEQ ID NO:74; o. Nucleotides at position 3417 to position 6368 of SEQ ID NO:72; p. Nucleotides at position 3416 to position 6367 of SEQ ID NO:74; q. Nucleotides at position 2015 to position 5235 of SEQ ID NO:126; r. Nucleotides at position 2015 to position 5231 of SEQ ID NO:126; s. Nucleotides at position 2019 to position 5167 of SEQ ID NO:127; t. Nucleotides at position 2019 to position 5235 of SEQ ID NO:128; u. Nucleotides at position 2016 to position 5249 of SEQ ID NO:128; or v. Nucleotides at position 2023 to position 5192 of SEQ ID NO:129.

6. The method of claim 1, wherein the targeted modification is introduced into a B. napus plant cell genome, and the method further includes generating a modified B. napus plant from the modified B. napus plant cell, wherein the modified B. napus plant has increased pod shatter tolerance relative to an unmodified B. napus that comprises the native BnIND, native BnALC, or native BnPGAZ gene sequence excised from the modified B. napus plant cell.

7. A modified B. napus plant, plant cell, or seed produced according to the method of claim 1.

8. A modified B. napus plant produced according to the method of claim 6.

9. A modified B. napus plant, plant cell or seed according to claim 7, wherein the plant, plant cell, or seed thereof comprises a deletion of BnIND-A genomic sequence corresponding to position 7740 to position 10346 of SEQ ID NO:56; BnIND-A genomic sequence corresponding to position 2018 to position 4639 of SEQ ID NO:69, BnIND-C genomic sequence corresponding to position 2676 to position 5101 of SEQ ID NO:57, BnIND-C genomic sequence corresponding to position 2019 to position 4441 of SEQ ID NO:67, BnIND-C genomic sequence corresponding to position 2018 to position 4446 of SEQ ID NO:70, BnALC-A genomic sequence corresponding to position 1723 to position 2849 of SEQ ID NO:71, BnALC-A genomic sequence corresponding to position 1722 to position 2851 of SEQ ID NO:73, BnALC-A genomic sequence corresponding to position 6369 to position 7511 of SEQ ID NO:72, BnALC-C genomic sequence corresponding to position 6368 to position 7510 of SEQ ID NO:74, BnALC-C genomic sequence corresponding to position 3417 to position 6368 of SEQ ID NO:72, BnALC-C genomic sequence corresponding to position 3416 to position 6367 of SEQ ID NO:74, BnPGAZ-A genomic sequence corresponding to position 2720 to position 5866 of SEQ ID NO:60, BnPGAZ-A genomic sequence corresponding to position 2720 to position 5865 of SEQ ID NO:60, BnPGAZ-A genomic sequence corresponding to position 2015 to position 5235 of SEQ ID NO:126, BnPGAZ-A genomic sequence corresponding to position 2015 to position 5231 of SEQ ID NO:126, BnPGAZ-A genomic sequence corresponding to position 2019 to position 5235 of SEQ ID NO:128, BnPGAZ-A genomic sequence corresponding to position 2016 to position 5249 of SEQ ID NO:128, BnPGAZ-C genomic sequence corresponding to position 2705 to position 5804 of SEQ ID NO:61, BnPGAZ-C genomic sequence corresponding to position 2704 to position 5802 of SEQ ID NO:61, BnPGAZ-C genomic sequence corresponding to position 2705 to position 5802 of SEQ ID NO:61, BnPGAZ-C genomic sequence corresponding to position 2019 to position 5167 of SEQ ID NO:127, or BnPGAZ-C genomic sequence corresponding to position 2023 to position 5192 of SEQ ID NO:129.

10. The modified B. napus plant of claim 8, wherein the plant comprises at least one excised allele of the BnIND-A gene (SEQ ID No:105), BnIND-C gene (SEQ ID No:108), or combinations thereof; at least one excised allele of the BnALC-A gene (SEQ ID No:120), BnALC-C gene (SEQ ID No:121), or combinations thereof; or at least one excised allele of BnPGAZ-A gene (SEQ ID No:112), BnPGAZ-C gene (SEQ ID No:116), or combinations thereof.

11. The modified B. napus plant of claim 7, wherein the plant comprises three or four excised alleles of the BnIND gene; three or four excised alleles of the BnALC gene; or three or four excised alleles of the BnPGAZ gene.

12. The modified B. napus plant of claim 7, wherein the plant has increased pod shatter tolerance relative to an unmodified B. napus that comprises the native BnIND, BnALC, or BnPGAZ genomic sequence deleted in the modified B. napus plant.

13.-15. (canceled)

16. A method of introducing a natural deletion of the BnIND-A gene into a B. napus plant comprising: a. crossing a first parent B. napus plant which is a plant comprising the targeted genome modification in accordance with claim 7 with a second B. napus plant comprising a native BnIND-A deletion to produce hybrid progeny plants; and b. selecting hybrid progeny plants that have both the targeted genome modification of the first the native BnIND-A deletion and the targeted genome modification.

17. The method of claim 16 further comprising: c. crossing the selected progeny plants with the first parent B. napus plant to produce backcross progeny plants; d. selecting for backcross progeny plants that have the natural deletion of the BnIND-A gene to produce selected backcross progeny plants; and e. repeating steps (c) and (d) to produce another generation of backcross progeny plants.

18. The method of claim 17 further comprising: f. repeating steps (c) and (d) three or more times to produce backcross progeny plants that comprise the natural deletion of the BnIND-A gene and the agronomic characteristics of the first parent plant when grown in the same environmental conditions.

19. A method for detecting the natural deletion of a BnIND-A gene in a B. napus plant, the method comprising: a. performing a first PCR assay using a first probe of SEQ ID NO:137, a first forward primer of SEQ ID NO:135, and a first reverse primer of SEQ ID NO:136 on a polynucleotide from a B. napus plant sample; b. performing a second PCR assay using a second probe of SEQ ID NO:140, a second forward primer of SEQ ID NO:135, and a second reverse primer of SEQ ID NO:139 on the polynucleotide sample; c. quantifying the first and second probe; and, d. comparing the quantified first and second probe to determine zygosity.

20. A PCR assay method for detecting the natural deletion of a BnIND-A gene in a B. napus plant, the method comprising; a. performing a first PCR assay using of first forward primer of SEQ ID NO:131, and a first reverse primer of SEQ ID NO:132 on a polynucleotide from a B. napus plant sample; b. performing a second PCR assay using a second forward primer of SEQ ID NO:133, and a second reverse primer of SEQ ID NO:134 on the polynucleotide sample; and c. quantifying the first and second resultant amplicon and comparing the quantified first and second resultant amplicon to determine zygosity.